LOCUS       DAVHIT010000001        46563 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVHIT010000001 DAVHIT010000000
VERSION     DAVHIT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.001"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..182
                     /locus_tag="VVN16_000001"
     CDS             <1..182
                     /locus_tag="VVN16_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU5571464.1"
                     /translation="LELIQGGADPYGKNPNGRYDWEIEPVLTLPVHGFCPRGTYDLGY
                     IGGGNHLCKGSAARF"
     gene            236..907
                     /locus_tag="VVN16_000002"
     CDS             236..907
                     /locus_tag="VVN16_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5571465.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(959..1156)
                     /locus_tag="VVN16_000003"
     CDS             complement(959..1156)
                     /locus_tag="VVN16_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5571466.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1149..2057
                     /locus_tag="VVN16_000004"
     CDS             1149..2057
                     /locus_tag="VVN16_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5571467.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2054..2515
                     /locus_tag="VVN16_000005"
     CDS             2054..2515
                     /locus_tag="VVN16_000005"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5571468.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2505..3392
                     /locus_tag="VVN16_000006"
     CDS             2505..3392
                     /locus_tag="VVN16_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5571469.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3395..5278
                     /gene="cbpE"
                     /locus_tag="VVN16_000007"
                     /gene_synonym="pce"
     CDS             3395..5278
                     /gene="cbpE"
                     /locus_tag="VVN16_000007"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU5571470.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIELYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5358..6488
                     /locus_tag="VVN16_000008"
     CDS             5358..6488
                     /locus_tag="VVN16_000008"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU5571471.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6498..7760
                     /locus_tag="VVN16_000009"
     CDS             6498..7760
                     /locus_tag="VVN16_000009"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU5571472.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7764..8561
                     /gene="proC"
                     /locus_tag="VVN16_000010"
     CDS             7764..8561
                     /gene="proC"
                     /locus_tag="VVN16_000010"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU5571473.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8781..9419
                     /locus_tag="VVN16_000011"
     CDS             8781..9419
                     /locus_tag="VVN16_000011"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5571474.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKSVLFDGMGLAK"
     gene            9416..10306
                     /locus_tag="VVN16_000012"
     CDS             9416..10306
                     /locus_tag="VVN16_000012"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU5571475.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10346..10663
                     /gene="yabA"
                     /locus_tag="VVN16_000013"
     CDS             10346..10663
                     /gene="yabA"
                     /locus_tag="VVN16_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU5571476.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10666..11544
                     /gene="rsmI"
                     /locus_tag="VVN16_000014"
     CDS             10666..11544
                     /gene="rsmI"
                     /locus_tag="VVN16_000014"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU5571477.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQHGMKKNQAIKEVAKYYQWNKS
                     QLYAAYHEWEENQEND"
     gene            11731..13065
                     /gene="trmFO"
                     /locus_tag="VVN16_000015"
     CDS             11731..13065
                     /gene="trmFO"
                     /locus_tag="VVN16_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU5571478.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPSLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            13149..13886
                     /locus_tag="VVN16_000016"
     CDS             13149..13886
                     /locus_tag="VVN16_000016"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5571479.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            13895..14452
                     /gene="frr"
                     /locus_tag="VVN16_000017"
     CDS             13895..14452
                     /gene="frr"
                     /locus_tag="VVN16_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5571480.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            14512..15366
                     /gene="cvfB"
                     /locus_tag="VVN16_000018"
     CDS             14512..15366
                     /gene="cvfB"
                     /locus_tag="VVN16_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU5571481.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDLTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            15375..15590
                     /locus_tag="VVN16_000019"
     CDS             15375..15590
                     /locus_tag="VVN16_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU5571482.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            15676..16644
                     /locus_tag="VVN16_000020"
     CDS             15676..16644
                     /locus_tag="VVN16_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU5571483.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEETACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQIKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(16622..16801)
                     /locus_tag="VVN16_000021"
     CDS             complement(16622..16801)
                     /locus_tag="VVN16_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571484.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            16837..17337
                     /locus_tag="VVN16_000022"
     CDS             16837..17337
                     /locus_tag="VVN16_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5571485.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNCRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            17340..17600
                     /locus_tag="VVN16_000023"
                     /pseudo
     CDS             17340..17600
                     /locus_tag="VVN16_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(17967..18293)
                     /locus_tag="VVN16_000024"
     CDS             complement(17967..18293)
                     /locus_tag="VVN16_000024"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571486.1"
                     /translation="MVDVAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(18239..18652)
                     /locus_tag="VVN16_000025"
     CDS             complement(18239..18652)
                     /locus_tag="VVN16_000025"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5571487.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(18653..19078)
                     /locus_tag="VVN16_000026"
     CDS             complement(18653..19078)
                     /locus_tag="VVN16_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU5571488.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVADRMAV"
     gene            19255..19824
                     /locus_tag="VVN16_000027"
     CDS             19255..19824
                     /locus_tag="VVN16_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5571489.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            19892..20542
                     /locus_tag="VVN16_000028"
     CDS             19892..20542
                     /locus_tag="VVN16_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU5571490.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            20526..22766
                     /locus_tag="VVN16_000029"
     CDS             20526..22766
                     /locus_tag="VVN16_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU5571491.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKTEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFMVELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            23165..23752
                     /locus_tag="VVN16_000030"
     CDS             23165..23752
                     /locus_tag="VVN16_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571492.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            23756..24940
                     /locus_tag="VVN16_000031"
     CDS             23756..24940
                     /locus_tag="VVN16_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571493.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPILFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVNLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKEFFN
                     RF"
     misc_feature    25104..25235
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            25251..25781
                     /gene="infC"
                     /locus_tag="VVN16_000032"
     CDS             25251..25781
                     /gene="infC"
                     /locus_tag="VVN16_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5571494.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            25814..26014
                     /gene="rpmI"
                     /locus_tag="VVN16_000033"
     CDS             25814..26014
                     /gene="rpmI"
                     /locus_tag="VVN16_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5571495.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            26066..26425
                     /gene="rplT"
                     /locus_tag="VVN16_000034"
     CDS             26066..26425
                     /gene="rplT"
                     /locus_tag="VVN16_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5571496.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            26483..26863
                     /locus_tag="VVN16_000035"
     CDS             26483..26863
                     /locus_tag="VVN16_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5571497.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            27113..27913
                     /locus_tag="VVN16_000036"
     CDS             27113..27913
                     /locus_tag="VVN16_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU5571498.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            27924..28862
                     /locus_tag="VVN16_000037"
     CDS             27924..28862
                     /locus_tag="VVN16_000037"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU5571499.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSTEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            28904..31069
                     /gene="lytB"
                     /locus_tag="VVN16_000038"
     CDS             28904..31069
                     /gene="lytB"
                     /locus_tag="VVN16_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU5571500.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYNSHSQAWYYFKSGGYMTANEWIWDKESWFY
                     LKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAA
                     YYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQ
                     ALDASKDFIPYYESDGHRFYHYVAQNASIPVAFHLSDMEVGKKYYSADGLHFDGFKLE
                     NPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHS
                     ALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGR
                     TFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(31130..32785)
                     /gene="pavA"
                     /locus_tag="VVN16_000039"
     CDS             complement(31130..32785)
                     /gene="pavA"
                     /locus_tag="VVN16_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU5571501.1"
                     /translation="MSFDGFFLHHIVEELRNELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            32890..33387
                     /gene="ybeY"
                     /locus_tag="VVN16_000040"
     CDS             32890..33387
                     /gene="ybeY"
                     /locus_tag="VVN16_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5571502.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            33368..33763
                     /locus_tag="VVN16_000041"
     CDS             33368..33763
                     /locus_tag="VVN16_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU5571503.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            33780..34679
                     /gene="era"
                     /locus_tag="VVN16_000042"
     CDS             33780..34679
                     /gene="era"
                     /locus_tag="VVN16_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU5571504.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            34728..35552
                     /gene="mutM"
                     /locus_tag="VVN16_000043"
     CDS             34728..35552
                     /gene="mutM"
                     /locus_tag="VVN16_000043"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU5571505.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            35552..36157
                     /locus_tag="VVN16_000044"
     CDS             35552..36157
                     /locus_tag="VVN16_000044"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5571506.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKSGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            36144..37343
                     /locus_tag="VVN16_000045"
     CDS             36144..37343
                     /locus_tag="VVN16_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU5571507.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            37343..37492
                     /gene="rpmG"
                     /locus_tag="VVN16_000046"
     CDS             37343..37492
                     /gene="rpmG"
                     /locus_tag="VVN16_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5571508.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            37532..37765
                     /gene="secG"
                     /locus_tag="VVN16_000047"
     CDS             37532..37765
                     /gene="secG"
                     /locus_tag="VVN16_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5571509.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            37867..40221
                     /gene="rnr"
                     /locus_tag="VVN16_000048"
     CDS             37867..40221
                     /gene="rnr"
                     /locus_tag="VVN16_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU5571510.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            40184..40651
                     /gene="smpB"
                     /locus_tag="VVN16_000049"
     CDS             40184..40651
                     /gene="smpB"
                     /locus_tag="VVN16_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5571511.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            40667..41527
                     /gene="tehB"
                     /locus_tag="VVN16_000050"
     CDS             40667..41527
                     /gene="tehB"
                     /locus_tag="VVN16_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU5571512.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTKPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDIPVDLYDINSASIGQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            41600..42553
                     /gene="coiA"
                     /locus_tag="VVN16_000051"
     CDS             41600..42553
                     /gene="coiA"
                     /locus_tag="VVN16_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU5571513.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            42572..44383
                     /gene="pepF"
                     /locus_tag="VVN16_000052"
     CDS             42572..44383
                     /gene="pepF"
                     /locus_tag="VVN16_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU5571514.1"
                     /translation="MVLQRNERNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVA
                     QYEGHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLY
                     SQLDQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELL
                     AGAGEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAY
                     QALYATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAA
                     VRKHLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGE
                     DYLSRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETG
                     HSMHSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLD
                     GFRGTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQY
                     EWARIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVM
                     RKAGVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            44385..45098
                     /locus_tag="VVN16_000053"
     CDS             44385..45098
                     /locus_tag="VVN16_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU5571515.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            45165..46106
                     /gene="prsA"
                     /locus_tag="VVN16_000054"
     CDS             45165..46106
                     /gene="prsA"
                     /locus_tag="VVN16_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU5571516.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(46223..46563)
                     /locus_tag="VVN16_000055"
                     /pseudo
     CDS             complement(46223..46563)
                     /locus_tag="VVN16_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        13943 a         8548 c        10653 g        13419 t
ORIGIN      
        1 aattagagct gatacaaggt ggagcagatc catatggtaa aaatcctaat ggtaggtacg
       61 attgggaaat agaaccagta ttaactctgc cggttcatgg attttgtccc agaggcacct
      121 atgatttagg atatattgga ggaggcaatc atctttgcaa aggaagtgct gcgagatttt
      181 aagtaaaatt tattagtaat atgaagaaac aagtggagaa agcagagaat ttaatatgaa
      241 aaaacgggct attcaaattt tactagcatt gtccttaatt ttttacaaat caacttggtt
      301 ttggagagtt ttcaatcacc tcgcaaagcc ctatctacca gcaagtcgtg aattttttca
      361 gattctgctt ttgatggaga gcggagttct tttcttagcg gtcatctatc tactggtttt
      421 tgcaggaaag aaaatttttc atttcaagtg gcagctgagg tacttcatct accttttact
      481 gggctacatc atttcatata tgtctgactt cctcttttcg tatttcatat ccctgtcttc
      541 aaatcagatt tctttgaatg aaacggtaga aatgatgggg agacaggagt tcccttattt
      601 cttgctcatc gtttgcttca tcgcccctat tgctgaggaa ttgatttatc gaggtgtgct
      661 tatgacaacc tttttcaaaa actcaccttg gtacggagat gttttgcttt ctgctattat
      721 tttcggttat attcatatca attttgcttt aactcctctt gcttttttca tttatgctag
      781 tggaggtctt attttagctc tattgtatcg catgactaaa aatctctact atccaatact
      841 agttcatatt ctcatcaata tcactgcctt ctgggatgtt tggttactcc tattttcagg
      901 aagttagctt actaaaaaaa tgtcggaatt ttccggcatt ttcttttttc acaaatagtc
      961 aacgtttttc tttccgatac tgaagtggtg tgtagccact tatttttttg aattgatttt
     1021 gaaaataaga ttggcgtgag aaaggcagat agtgaagata gttaagaaga ataggatgtt
     1081 cttttttcct ttttggaaaa cttctaaaat atggtataat gaaaagataa agaagttggg
     1141 ggtagaagat gaacattcaa caattacgct atgttgtggc tattgccaat agtggtactt
     1201 ttcgtgaagc tgctgaaaag atgtatgtta gtcagccgag tctgtctatt tctgttcgtg
     1261 atttggaaaa agagttgggc tttaagattt tccgtcggac cagctcaggg actttcttga
     1321 cccgtcgtgg gatggaattt tatgaaaaag cgcaagaatt ggttaaagga tttgatattt
     1381 ttcaaaatca gtatgccaat cctgaagaag aaaaagatga attttccgtt gctagccagc
     1441 actatgactt cttaccacca actattacgg ccttttcaga gcgctatcct gactataaga
     1501 acttccgtat ttttgaatca actactgttc aaatattaga tgaagtggcg caagggcata
     1561 gtgagattgg gattatctac ctcaacaatc aaaataaaaa ggggattatg caacgggttg
     1621 aaaagttagg tctggaggtc atcgaattga ttcctttcca tacccatatt tatctctgtg
     1681 agggtcatcc tttagctcag aaagaggaat tagtcatgga ggatttagcg gatttaccaa
     1741 cggttcgttt cactcaagag aaagacgagt acctttatta ttcagagaac tttgtcgata
     1801 ccagcgctag ctcacagatg tttaatgtga cagaccgtgc caccttgaat ggtattttgg
     1861 agcggacgga cgcctatgcg acaggttctg gatttttaga tagtgacagt gttaatggca
     1921 ttacagttat tcgtctcaag gataacctag ataaccgcat ggtctatgtt aaacgtgaag
     1981 aagtggagct tagtcaagct gggactctct tcgtagaagt catgcaagaa tattttgatc
     2041 aaaagaggaa atcatgaaaa aaagagcaat agtggcagtc attgtactgc ttttaattgg
     2101 gctggatcag ttggtcaaat cctatatcgt ccagcagatt ccactgggtg aagtgcgctc
     2161 ctggattccc aatttcgtta gcttgaccta cctgcaaaat cgaggtgcag ccttttctat
     2221 cttacaagat cagcagctgt tattcgctgt cattactctg gttgtcgtga taggtgccat
     2281 ttggtattta cataaacaca tggaggactc attctggatg gtcttgggtt tgactctaat
     2341 aatcgcgggt ggtcttggaa actttattga cagggtcagt cagggctttg ttgtggatat
     2401 gttccacctt gactttatca actttgcaat tttcaatgtg gcagatagct atctgacggt
     2461 tggagtgatt attttattga ttgcaatgct aaaagaggaa ataaatggaa attaaaattg
     2521 aaactggtgg tctgcgtttg gataaggctt tgtcagattt gtcagaatta tcacgtagtc
     2581 tcgcgaatga acaaattaaa tcaggccagg tcttggtcaa tggtcaagtc aagaaagcta
     2641 aatacacagt ccaagagggt gatgtcgtca cttaccatgt gccagaacca gaggtattag
     2701 agtatgtggc tgaggatctt ccgctagaaa tagtctacca agatgaggat gtggctgtcg
     2761 ttaacaaacc tcagggaatg gttgtgcacc cgagtgctgg tcataccagt ggaaccctag
     2821 taaatgccct catgtatcat attaaggact tgtcgggtat caatggggtt ctgcgtccag
     2881 ggattgttca ccgtattgat aaggatacgt caggtcttct catgattgct aaaaacgatg
     2941 atgcgcatct agcacttgcc caagaactca aggataaaaa gtctctccgc aaatattggg
     3001 cgattgttca tggaaatctg cctaatgatc gtggtgtaat tgaagcgccg attggccgga
     3061 gtgaaaaaga ccgtaagaaa caggctgtaa ctgctaaagg gaagcctgca gtgacgcgtt
     3121 ttcacgtctt ggaacgcttt ggcgattata gcttagtaga gttgcaactg gagacagggc
     3181 gcactcatca aatccgtgtc cacatggctt atatcggcca ttcagtcgct ggtgatgagg
     3241 tctatggtcc tcgcaagact ttgaaaggac atggacaatt tcttcatgcc aagactttag
     3301 gttttactca tccgagaaca ggtaagacct tggaatttaa agcagatatc ccagagattt
     3361 ttaaggaaac cttggagaga ttgagaaagt aagaatgaaa aagaaattaa ctagtttagc
     3421 acttgtaggc gcttttttag gtttgtcatg gtatgggaat gttcaggctc aagaaagttc
     3481 aggaaataaa atccacttta tcaatgttca agaaggtggc agtgatgcga ttattcttga
     3541 aagcaatgga cattttgcca tggtggatac aggagaagat tatgatttcc cagatggaag
     3601 tgattctcgc tatccatgga gagaaggaat tgaaacgtct tataagcatg ttctaacaga
     3661 ccgtgtcttt cgtcgtttga aggaattggg tgtccaaaaa cttgatttta ttttggtgac
     3721 ccatacccac agtgatcata ttggaaatgt tgatgaatta ctgtctacct atccagttga
     3781 ccgagtctat cttaaaaaat atagtgataa tcgtattact aattctgaac gtctatggga
     3841 taatctgtac ggttatgata aggttttaca gactgcttca gaaaaaggtg tttcagttat
     3901 tcaaaatatc acacaagggg atgctcattt tcagtttggg gacatggata ttgagctcta
     3961 taactatgaa aatgaaaccg attcatcggg tgaattaaag aaaatttggg atgacaattc
     4021 caattccttg attagcgtgg tgaaagtcaa tggcaagaaa atttaccttg ggggcgattt
     4081 agataatgtt catggagcag aagacaagta tggtcctctc attggaaaag ttgatttgat
     4141 gaagtttaat catcaccatg ataccaacaa atcaaatacc aaggatttca ttaaaaattt
     4201 gagtccgagt ttgattgttc aaacttcgga tagtctacct tggaaaaatg gtgttgatag
     4261 tgagtatgtt aattggctca aagaacgagg aattgagaga atcaacgcag ccagcaaaga
     4321 ctatgatgca acagtttttg atattcgaca agacggttta gtaaacattt caacttccta
     4381 caagccgatt ccaagttttc aagctggttg gcataagagt tcatatggaa actggtggta
     4441 tcaagcgcct gattctacag gagaatatgc tgtcggttgg aatgaaatta agggtgaatg
     4501 gtattacttt aaccaaacgg gtatcttatt acagaatcaa tggaaaaaat ggaacaatcg
     4561 ttggttctat ttgacagact ctggtgttgc tgctaaaaat tggaagaaaa tcgctggaat
     4621 ctggtattat tttaacaaag aaaaccagat ggaaattggt tggattcaag ataaagagca
     4681 gtggtattat ttggatgttg atggctctat gaagacaggt tggcttcaat ataaggggca
     4741 atggtattac tttgctccat caggggaaat gaaaacgggc tgggtaaaag ataaagaagc
     4801 ctggtactat atggattcta ctggtatcat gaagacaggt gagatagaag ttgctggaca
     4861 tcattactat ctagaagaat caggagctat gaagcaaggc tggcttaaaa aggcaaatga
     4921 ttggtatttc tacaagacag atggttcacg agctataggt tggattaaag acaaggataa
     4981 atggtacttc ttgaaagaaa atggtcaatt acttgtgaat ggtaagacac cagaaggcta
     5041 tactgtggat tcaagtggtg cctggttagt ggatgttccg atcgagaaat ctgctacaat
     5101 taaaactaca agtcattcag aaataaaaga atccaaagaa gtagtgaaaa aggatcttga
     5161 aaataaagaa acgagtcaac atgaaagtgt tacaaattct tcaactagtc aagatttgac
     5221 atcctcaact tcacaaagct ctgaaacgag tgtaaacaaa tcggaatcag aacagtagta
     5281 gaaaagaagg ttttagggcc ttctttttcc tatcaactct tttctatttc ctgttattca
     5341 tgttataatg gataaatatg aataatcgga gtgagactat gaaatacaaa cggattgtct
     5401 ttaaggtggg tacttcttct ctgacaaatg aggatggaag tttatcacgt agtaaggtaa
     5461 aggatattac ccagcagttg gctatgctgc acgaggctgg tcatgagttg attttggtgt
     5521 cttcaggtgc cattgcggct ggttttggag ccttaggatt taaaaagcgt ccgactaaaa
     5581 ttgctgataa acaggcttca gcagcggtag ggcagggact tttgttggaa gaatatacga
     5641 ccaatcttct cttgcgtcaa atcgtttctg cacaaatctt gctgacccaa gatgactttg
     5701 tggataagcg tcgttataaa aatgcccatc aggctttgtc ggttttgctc aaccgtgggg
     5761 caattcctat catcaatgag aatgatagtg tcgttattga tgagctcaag gttggggaca
     5821 atgacactct aagtgctcaa gtagcggcga tggtccaagc agacctttta gttttcttga
     5881 cagatgtgga cggtctctat actggaaatc ctaattcaga tccaagagcc aaacgcttgg
     5941 agagaatcga gaccatcaat cgtgagatta ttgatatggc tggtggagca ggttcgtcaa
     6001 acggaactgg gggtatgtta accaaaatca aggctgcaac tatcgcgacg gaatcaggag
     6061 ttcctgttta tatctgctca tccttgaaat cagattccat gattgaggcg gcagaggaga
     6121 ccgaggatgg ttcttacttt gttgctcaag agaaggggct tcgtacccag aaacaatggc
     6181 ttgccttcta tgctcagagt caaggttcta tttgggttga taaaggggct gcggaagctc
     6241 tctctcaaca tggaaagagt cttctcttat ctggtatcgt tgaagcagaa ggagcctttt
     6301 cttacggtga tatcgtgaca gtatttgaca aggaaagtgg aaaatcactt ggaaaaggac
     6361 gcgtgcaatt tggagcatct gctttggagg atatgttgcg ttctcaaaaa gccaagggtg
     6421 tcttgattta ccgtgacgac tggatttcca ttactcctga aatccaacta ctttttacag
     6481 aattttagag gtaaactatg gtgagtagac aagaacaatt tgaacaggta caggctgtta
     6541 aaaaatcgat taacacagct agtgaagaag tgaaaaacca agccttgcta gccatggctg
     6601 atcacttagt ggctgctact gaggaaattt tagcggctaa tgccctcgat atggcagcgg
     6661 ctaaggggaa aatctcagat gtgatgttgg atcgtcttta tttggatgca gatcgtatag
     6721 aagcgatggc aagaggaatt cgtgaagtgg ttgccttacc agatccaatc ggtgaagttt
     6781 tagaaacaag tcagcttgaa aatggtttgg ttatcacaaa aaaacgtgta gctatgggtg
     6841 tcatcggtat tatctatgaa agccgtccaa atgtgacgtc tgatgcggct gctttgactc
     6901 ttaagagtgg aaatgcggtt gttcttcgta gtggtaagga tgcctatcaa acaacccatg
     6961 ccattgtcac agccttgaag aagggcttgg agacgactac tattcatcca aatgtgattc
     7021 aactggtgga ggatactagc cgtgaaagta gttatgctat gatgaaggcc aagggctatc
     7081 tagaccttct cattcctcgt ggaggagctg gcttgatcaa tgcagtggtt gagaatgcga
     7141 ttgtacctgt tatcgagaca gggactggga ttgtccatgt ctatgtggat aaggatgcag
     7201 acgaagacaa ggcgctgtct atcatcaaca atgctaaaac cagtcgtcct tctgtttgta
     7261 atgccatgga ggttctgctg gttcatgaaa acaaggcagc aagcatcctt cctcgcttgg
     7321 atcaaatgct ggttgcagag cgtaaggaag ctggactgga accaattcaa ttccgcttgg
     7381 atagcaaagc aagccagttt gtttcaggtc aagcagctga gacccaagac tttgacaccg
     7441 agtttttaga ctatgtcctt gctgttaagg ttgtgagcag tttagaagaa gcggttgcgc
     7501 acattgaatc ccacagcacc catcattcgg atgctattgt gacggaaaat gctgaagctg
     7561 cagcatactt tacagatcaa gtggactctg cagcggtgta tgttaatgcc tcaactcgtt
     7621 tcacagatgg aggacaattt ggtcttggtt gtgaaatggg gatttctact cagaaattgc
     7681 acgcgcgtgg tccaatgggc ttgaaagagt tgaccagcta caagtatgtg gttgctggtg
     7741 atgggcagat aagggagtaa gagatgaaga ttggatttat cggtttgggg aatatgggtg
     7801 ctagcttggc aaaatctgtc ttgcagacta ggacgtcaga tgagattctc cttgccaatc
     7861 gtagtcaagc taaggtagat gctttcattg cagactttgg tggtcaggct tccagcaatg
     7921 aagaaatgtt tgcagaagca gatgtgattt ttctaggagt taagcctgct cagttttctg
     7981 aactgctttc tcaataccag accatccttg aaaaaagaga aagtcttctt ttgatttcga
     8041 tggcagctgg attgacctta gaaaaactag caagtcttat cccaagtcaa caccgaatta
     8101 ttcgtatgat gcctaatacc cctgcttcta tcgggcaagg agtgattagt tatgccttgt
     8161 ctcctaattg cagggctgag gacagtgagc tcttttgtca gcttttatcc aaggctggtc
     8221 tcttggttga attaggagaa agcttaatca atgcagcgac aggtcttgca ggctgtggac
     8281 cagcctttgt ctatctcttt attgaagcct tggcagatgc aggtgttcag acgggattat
     8341 cacgagaaac agccttgaaa atggcagccc aaactgtggt aggagctggg caattggtcc
     8401 tagaaagcca gcaacatcct ggagttttga aagatcaagt ttgtagtcca ggcggttcga
     8461 ctattgctgg cgtagcaagc ctagaagcgc atgcttttcg aggcacagtc atggaggcag
     8521 ttcatcaagc ctataaacga acacaagaac taggtaaata agaggtagct ttatctgcct
     8581 cttttatggt ggttgatact cttcgaaaat caaattcaaa ctgcgtcagc ttcaccttgc
     8641 cgtactcaag tacagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
     8701 gaaatgagaa gacaaaaaga ttgtcacaaa cccctatttt tttgatagaa tagaagtagt
     8761 aaaaaagaaa tgagttagac atgtcaaaag gatttttagt ctctcttgag ggaccagagg
     8821 gagcaggcaa gaccagtgtt ttagaggctc tgctaccaat tttagaggaa aaaggagtag
     8881 aggtgttgac gacccgtgaa cctggcggag tcttgattgg ggagaagatt cgggaagtga
     8941 ttttggatcc aagtcatact cagatggatg ctaaaacaga gctacttctc tatattgcca
     9001 gtcgcagaca gcatttggtg gaaaaagttc ttccagccct tgaagctggc aagttggtca
     9061 tcatggatcg ttttatcgat agttctgttg cctatcaggg atttggtcgt ggcttagata
     9121 ttgaagccat tgactggctc aatcagtttg cgacagatgg cctcaaaccc gatttgacac
     9181 tctattttga catcgaggtg gaagaagggc tggctcgtat tgctgctaat agtgaccgcg
     9241 aggttaatcg tttggatttg gaagggttgg acttgcataa aaaagttcgt caaggctacc
     9301 tttctcttct ggataaagag ggaaatcgca ttgtcaagat tgatgctagt ctccctttgg
     9361 agcaagttgt ggaaactacc aagtctgtct tgtttgacgg aatgggcttg gccaaatgaa
     9421 acaagatcaa ctaaaggctt ggcaaccagc tcagtttgac cgttttgtcc gtatcttaga
     9481 acaagaccag ctcaatcacg cctatctctt ttcaggtttc tttggaagct tggaaatagc
     9541 gcaattttta gctaagagcc tcttttgtac ggataaagtt ggcgtcttac catgtgagaa
     9601 atgccgaagt tgcaagctga ttgaacagga agagtttcca gatgtcacct tgattaagcc
     9661 agtcaatcag gtcatcaaga cagaacgcat tcgggaattg gtgggacagt tttctcaagc
     9721 agggattgaa agccagcaac aggtctttat catcgagcaa gcggataaaa tgcatcccaa
     9781 cgcagccaat tctctgctca aggtcatcga agaaccccag agtgaagttt atattttctt
     9841 cttgactagc gatgaggaaa agatgttacc gacaatccga agtcggaccc agatcttcca
     9901 ctttaaaaag caagaagaaa aacttatctt actcttagaa caaatgggac ttgttaagaa
     9961 aaaagcgact cttttagcta agtttagtca atcgcgagct gaagcagaaa agttggctaa
    10021 tcaggcaagt ttttggacct tggtcgatga aagtgaacgc ctgctgactt ggttagtagc
    10081 taagaaaaaa gaaagttatc tacaggttgc caaattagcc aacttggcag atgataagga
    10141 aaaacaggat caggttttac ggattcttga agttctctgt gggcaggacc tcttgcaggt
    10201 aagagtaaga gtgattctac aagatttact agaagctaga aaaatgtggc aagctaatgt
    10261 cagctttcaa aatgccatgg aatatctggt cttgaaagaa atataaactc aaaaatgaat
    10321 gataaagaaa ggaaagggct gttttatgga caaaaaagaa ttatttgacg cgctggatga
    10381 tttttcccaa caattattgg taaccttagc cgatgtggaa gccatcaaga aaaatctcaa
    10441 gagcttggta gaagaaaata cagctcttcg cttggaaaat agtaagttgc gagaacgctt
    10501 gggtgaggtg gaagcagacg ctcccgtcaa ggccaagcat gttcgcgaaa gtgtccgtcg
    10561 tatttaccgt gatggatttc acgtatgtaa tgatttttat ggacaacgtc gagagcagga
    10621 cgaagaatgt atgttctgtg atgagttgtt atacagggag taagcatgca aattcaaaaa
    10681 agttttaagg ggcagtctcc ctatggcaag ctgtatctag tggcaacgcc gattggcaat
    10741 ctagatgata tgacttttcg tgctatccag accttgaaag aagtggactg gattgctgct
    10801 gaggatacgc gcaatacagg gcttttgctc aagcattttg acatttccac caagcagatc
    10861 agttttcatg agcacaatgc caaggaaaaa attcctgatt tgattggttt cttgaaagca
    10921 gggcaaagta ttgctcaggt ctctgatgcc ggtttgccta gcatttcaga ccctggtcat
    10981 gatttggtta aggcagctat tgaggaagaa attgcagttg ttacagttcc aggtgcctct
    11041 gcaggaattt ccgccttgat tgccagtggt ttagcgccgc agccacatat cttttacggc
    11101 tttttaccga gaaaatcagg ccagcaaaag caatttttcg actcgaaaaa agactatcct
    11161 gaaactcaga ttttttatga atcgccccat cgtgtggcag atacattgga aaatatgcta
    11221 gaagtctacg gtgaccgctc ggtagtcttg gtcagggaat tgactaaaat ctatgaagaa
    11281 taccaaagag gtaccatttc tgaattgctg gaaagcatct ctgaaacgtc tctcaagggt
    11341 gaatgccttc ttattgttga aggtgccagt caggatgtgg aggaaaaaga cgaggaggac
    11401 ttgttcttag aaatccaagc ccgcatccaa catggtatga agaaaaatca agccattaag
    11461 gaagttgcga aatactacca gtggaataaa agtcagcttt atgctgccta ccacgaatgg
    11521 gaagaaaatc aagaaaatga ctaaacaggg aagtttgcct tcttcccttt ttttgttata
    11581 ctagtagaag aaaaaaatag aaagatttgt gggagtgaaa aagtcctgtg gactttttca
    11641 gcctgagcca agaaactcga aagctcgtaa gtctgattgg ctttttcaat gtgaatctta
    11701 acttcacact cccaaagagg tattagtgtc gtgtctcaat cttatatcaa tgttatcggt
    11761 gctggtttgg caggttctga agcagcttac caaatcgcag agcgtggtat tccagttaaa
    11821 ctttatgaaa tgcgtggtgt caagtcgact cctcagcata aaacagacaa ttttgctgag
    11881 ttggtttgtt ccaattcttt gcgtggggat gctttgacaa atgcagttgg tcttctcaag
    11941 gaagaaatgc gtcgcttggg ttctgttatc ttggaatctg ctgaggctac acgtgttcct
    12001 gccggtggag cccttgcggt ggatcgtgat ggtttctccc aaatggtaac cgaaaaagtg
    12061 gtcaaccacc ccttgattga agtggtgcgt gatgaaatta cagaattgcc aacagatgtt
    12121 attacagttg tggcgaccgg tcccttgact agcgatgccc tggctgaaaa gattcatgcc
    12181 cttaatgatg gcgatggctt ctatttctac gatgcagcag cgcctattat cgatgtcaac
    12241 actatcgata tgagtaaggt ctatctcaaa tctcgttatg ataagggaga agcagcctat
    12301 ctcaatgctc caatgaccaa gcaagagttt atggatttcc atgaagcctt ggtcaatgcg
    12361 gaagaagcac cgctcaattc ttttgaaaaa gaaaagtact ttgaaggatg tatgccaatc
    12421 gaagttatgg ccaaacgtgg cattaaaact atgctttatg gccctatgaa gccagtcggt
    12481 cttgagtatc cggacgacta cacaggacct cgtgatggag aatttaaaac accttatgcg
    12541 gttgtgcaac ttcgtcagga taatgcagct ggtagcctct acaatatcgt tggtttccag
    12601 acccacctca aatggggaga acaaaagcgt gtcttccaaa tgattccgag tcttgaaaat
    12661 gcggagtttg tccgttatgg tgtcatgcat cgcaattctt acatggattc accaaatctt
    12721 cttgagcaga cttaccgttc caagaaacaa ccaaatctct tctttgctgg tcagatgacg
    12781 ggggtggaag gctatgttga gtcggcggct tcgggcttag ttgcgggaat taacgcagct
    12841 cgtctcttca aggaagaaag cgaggttatt ttccccgaga cgacagcgat tggaagctta
    12901 gctcattaca ttacccatgc cgacagcaaa catttccaac caatgaatgt caattttggg
    12961 atcatcaagg agttggaagg cgagcgtatc cgtgataaga aggctcgtta tgaaaaaatt
    13021 gcagagcgtg cccttgccga cttagaggaa tttttgactg tctaattttt ttgaaagaat
    13081 tgctcatgat actataaaaa tcttagaaat tgtgataaaa taggtaggat gaaagaagga
    13141 gagtgaaaat ggcgaatccc aagtataaac gtattttaat caagttatca ggtgaagccc
    13201 ttgccggtga acgtggcgta gggattgata tccaaacagt tcaaacaatc gcaaaagaga
    13261 ttcaagaagt tcatagctta ggtatcgaaa ttgcccttgt tatcggtgga ggaaatctct
    13321 ggcgtggaga acctgcagca gaagcaggta tggaccgtgt tcaggcagat tacactggaa
    13381 tgcttgggac tgttatgaat gctcttgtga tggcagattc attgcaacaa gttggggttg
    13441 atacgcgtgt acaaacagct attgctatgc aacaagtggc agagccttat gtccgtggac
    13501 gtgcccttcg tcaccttgaa aaaggccgta tcgttatctt tggtgctgga attggttcac
    13561 catacttctc gacagataca acagcggccc ttcgtgcagc tgaaatcgaa gcagatgcca
    13621 tcctcatggc taaaaatggc gtcgatggtg tgtacaatgc cgatcctaag aaggacaaga
    13681 cagccgttaa gtttgaagaa ttgacccacc gtgatgttat caacaaaggt cttcgtatca
    13741 tggactcaac agcctcaacc ctctcaatgg acaacgacat tgacttggtt gtcttcaaca
    13801 tgaaccaatc aggcaacatc aaacgtgtcg tatttggtga aaatatcgga acaacagttt
    13861 caaataatat cgaagaaaag gaataagaaa gaatatggct aacgtaatta ttgaaaaagc
    13921 taaagagaga atgacccagt ctcaccaatc acttgctcgt gaatttggtg gtatccgtgc
    13981 tggtcgtgcc aatgcaagct tgcttgaccg tgtacatgta gaatactatg gagtcgaaac
    14041 tcctcttaac caaatcgctt caattacgat tccagaagcg cgtgttttgt tggtaacacc
    14101 atttgacaag tcttcattga aagacatcga acgtgccttg aacgcttctg atcttggtat
    14161 cacaccggct aatgacggtt ctgtgattcg cttggttatc ccagctctta cagaagaaac
    14221 tcgtcgtgac cttgctaaag aagtgaagaa ggtcggcgaa aatgctaaag tggctgtccg
    14281 caatatccgt cgcgatgcta tggacgaagc taagaaacaa gaaaaagcaa aagaaatcac
    14341 tgaagacgaa ttgaagactc ttgaaaaaga tattcaaaaa gtaacagacg atgctgttaa
    14401 acacatcgac gacatgactg ctaacaaaga gaaagaactt ttggaagtct aaaaataaac
    14461 agaaaaactc agttggcatt gctggctgag ttttattcga aagaaggaaa tatgaataca
    14521 aatcttgcaa gttttatcgt tggactgatc atcgatgaaa atgaccgttt ttactttgtg
    14581 caaaaggatg gtcaaaccta tgctcttgct aaggaagagg gccaacatac agtaggggat
    14641 acggtcaaag gttttgcata cacggatatg aagcaaaaac tccgcctgac aaccttagaa
    14701 gtgactgcca ctcaggacca atttggttgg ggacgtgtca cagaggttcg taaggacttg
    14761 ggtgtctttg tggatacagg ccttcctgac aaggaaatcg ttgtgtcact cgatattctc
    14821 cctgagctca aggaactctg gcctaagaag ggcgaccaac tctacatccg tcttgaagtg
    14881 gataagaaag accgtatctg gggcctcttg gcttatcaag aagacttcca acgtcttgct
    14941 cgtcctgctt acaacaacat gcagaaccaa aactggccag ccattgttta ccgtctcaag
    15001 ctgtcaggaa cttttgttta cctaccagaa aataatatgc ttggttttat tcatcctagc
    15061 gagcgttacg cagagccacg tttggggcaa gtattagatg cgcgcgttat tggtttccgt
    15121 gaagtggacc tcactctgaa cctctccctc aaaccacgct cctttgaaat gttggaaaac
    15181 gatgctcaga tgattttgac ttatttggaa agcaatggcg gtttcatgac cttaaatgat
    15241 aagtcatctc cagacgacat caaggcaacc tttggcattt ctaaaggtca gttcaagaaa
    15301 gctttaggtg gtcttatgaa ggctggtaaa atcaagcaag accagtttgg gacagagttg
    15361 atttagggag atttatgaga aaatcatttt acacttggct catgaccgag cgcaatccta
    15421 aaagtaacag tcccaaagct atcttggcag acctcgcttt tgaagaggca gcttttccaa
    15481 aacacacaga tgattttgat gaggtcagtc gctttttgga ggagcatgcc agtttctctt
    15541 ttaacctagg agactttgac gctatctggc aagaatactt agaacactag catttattca
    15601 ctaggtttgg gctagtaatt tctccatccc tctgctataa taaaaagaaa taaaaggatt
    15661 agagaggttc tttatttgaa ggaacattca atagacattc aactgagtca tccagatgac
    15721 ttgtttcatc tttttggttc caatgaacgc catcttcgtt tgatggaaga agagctggat
    15781 gtggtgattc atgctcgtac ggagattgtc caggttttgg gagaagagac tgcctgtgag
    15841 gaagcccgtc aggttatcca agctttgatg gtcttggtga accgtgggat gaccgttggt
    15901 acgccagatg tggtgactgc aattagcatg gtcaaaaacg atgaaattga taagtttgtc
    15961 gccctttacg aagaagaaat tatcaaggat aatactggga aaccgatccg cgttaaaacg
    16021 ttaggtcaaa aactttatgt ggacagtgtc aaacagcatg atgtgacctt tggaattggg
    16081 cctgcaggga cggggaagac ctttcttgca gttactttgg cagtgactgc ccttaaacgt
    16141 gggcaaatca agcggattat cttgacacgt ccagcggtgg aagcaggaga gagtctaggt
    16201 tttcttccgg gtgatcttaa ggagaaggta gatccttacc ttagacctgt ttatgatgcc
    16261 ttgtatcaga ttctggggaa agaccaaaca actcgactca tggagcgtga aattatcgag
    16321 attgcgcccc ttgcctatat gcgtggacgg accttggatg atgcctttgt cattctcgat
    16381 gaggcgcaaa atacgaccat catgcagatg aagatgttct tgactcgttt aggttttcat
    16441 tctaagatga ttgtcaatgg agatattagt cagattgacc tgccacgtaa tgtcaagtcc
    16501 ggtttgattg atgctcaaga gaaactcaag aacatccatc agattgactt tgttcatttt
    16561 tcagccaagg atgtggttcg ccatccagtt gtcgctcaga ttatccgagc ctatgaatat
    16621 tctactgaag ttgcacacga ctgattttga ggaagttcgc ctgcaaaaga atatactttt
    16681 gagagaggaa aatccagttg tataggctaa aggttttatc caaaggtttg agacaacgat
    16741 taggcacgat ggaaagaact tttatgtggc tgatgacgat cagtgcatct tcctgtgtca
    16801 taatcacagg gcacaagaaa gtaggaattt gaaaagatga ttgaccaact atctaagtat
    16861 tacagttgta ggatactaac tgaaaaggat attccaagta ttttatcttt atatgaaagt
    16921 aatcctctgt attttcagca ttgtccacca gagccaaatt ttgcaactgt aaaagaggac
    16981 atgctttgtc tacctgaagg taaagctaag gctgataagt tttttgttgg attttggaat
    17041 ggatctgacc ttgtggctgt tatggatttt gtctatgcat atcctgatga ggagactgtt
    17101 tttattggtt tgtttatggt tgatcaagcc tatcagagga aagggattgg tagtcatatt
    17161 gtgacagaag cactagctta ttttgctaag aactgtcgaa aggcacgttt ggcttatgtt
    17221 aagggaaatc cgcaatctca gcatttttgg gaaaagcagg gctttaaatc aattggatgc
    17281 gaggttaagc aagaactcta tacggttgtt atcgctgaac agagcctaga agattagaaa
    17341 tggcatcaag taagaactat ttggaatttg ttttggaaca attatcagga ttagatgatg
    17401 tgacttaccg ttccatgatg ggggagtata ttctttactt ccgcggcaag attattggcg
    17461 gcatttatga cgatcgcttt ttagttaaac ccgtgcaagc agtcttagat aagattgacc
    17521 aatcttcttt tgagtttcca tacaaaggtg ccaaagaaat gatttgagtg gaagaacttg
    17581 ataataagat gtttctataa gacctgattt tagctatgta taaccaactg ccaacgccca
    17641 aacctaaaaa gaaaaagcaa gggtgaacga agtaaaaaag aagtctgcta aggccctgtc
    17701 tttgcacggg taaaatttta tatataaaaa gaagctggga ctaaagagct cagcttcctt
    17761 tggtttatat aattgtcatt acaagacgaa gtggttgggc gaaactctgt tgattttatt
    17821 caatttagag tttcttatgc acaattgagt ctggaacgaa agtctccagt tgcaaagtat
    17881 acagtacaat aaaccaacga tgtaatagct gatgacacaa agcacagtgg gtaggacttg
    17941 cgaagtcacc cttttctttt caaaatttat actaaatcat tgatatcagt gtagtcacga
    18001 ttaagtcctt gagcaactgg taggctagtt aagtaacctt gataagtggt cacaccttga
    18061 cgcaagcctt catcttcaga gattgcttgt gcgaatcctt tgccagccaa agcttcgata
    18121 taaggaagag tgacattggt tagggcgatg gttgaagtgc gagcaaccgc accagggata
    18181 ttggcaacgg catagtggag aacaccgtgt ttttcataga cgggttcatc gtgcgttgtc
    18241 acacggtcag ctgtttcgat aaggccacct tggtcaacag caacgtcaac gatacagagc
    18301 ctggacgcat ttgtttgacc atctcatctg tcaccaattc cggtgctttt gcaccaggga
    18361 tgagaatggc tccaatcacc acatcagcat ctctcacact tgcttcaatg ttgaatgaat
    18421 tagatataag agtttgaatt tgacttccaa agacttcttc tagaactgag agacgcttgg
    18481 cactaatatc taaaatagtc acttgagcac caagaccaag ggcgatgcgg gcagcatgtg
    18541 taccgacgac accatcaccg atgatagtta cttttccttt tggaacacct ggtacaccac
    18601 caggtagaac accagatcca ccagcttgct tagtaaggaa gtgagctccg atttaaacag
    18661 ccatacgatc tgcaacctca ctcataggaa cgaggagcgg tagttgtcct tgattgtcac
    18721 gaacagtttc ataggcaatt cctgttgttt ttgctgctaa catagcatct gctaattctg
    18781 gagcagcggc catgtgcaag taggtgaaga gaagaagatc gtcgcgcaag taaccgtatt
    18841 cagaacttaa aggttctttt actttcacaa ccaactctgc tgcccaagct tcaccagcag
    18901 tagcgacaat ctcagctcct tgcttttgat agtcagcatc agtaaagcca gaaccgagac
    18961 cagcatttgt ttcgataagg acacgatgac cacgactaac taagctatga acacctgcag
    19021 gtgtgagggc gacacggttt tcgttatttt taatttcttt tgggattccg attaacattg
    19081 agataaccta cctttcaatt gacggtcttg ttttggttgt cacattccag ttcataaatc
    19141 aaaaatgtga cggtttcatt gtatatgaaa ccgcttcaaa aatcaagaaa aacttgtcat
    19201 ccaaattttt tatgctagac tagtgaaaat caagctttga ttggttggaa aagtatggaa
    19261 tcaatttttg taaaacttgc ccagtatccg tctatagaaa cggagcgttt attgcttaga
    19321 cctgtaactt tggatgatgc ggaagcaatg tttgactatg cctcggacaa gggtaataca
    19381 cgttacactt ttccaaccaa tcaaagcttg gaagaaacca agaataacat tgctcagttc
    19441 tatttggcta atcccttggg acgttgggga atagaactaa aaagcaatgg tcagtttatt
    19501 ggaaccattg acttgcacaa gattgattct gttcttaaga aggcagctat tggctacatt
    19561 atcaataaaa agtattggaa tcaaggatta acgacagaag ccaatcgtgc tgtgattgag
    19621 ctagcttttg agaagatagg gatgaataag ttgactgccc ttcacgataa ggacaatcct
    19681 gcgtcaggaa aggtcatgga gaaatcaggc atgcgttttt cccatgcaga accatatgct
    19741 tgtatggacc agcatgaaaa agatcgaatc gttacaagag ttcattatgt cttgaccaag
    19801 gaagactatt ttgcaaataa ataagcagtt gaaaagaaat ttttcgactg ttttttcttc
    19861 ctcttacgaa taatctaaga gaggagaaaa tatggaagca attatcgaga aaatcaaaga
    19921 gtataaaatc atcgtcatct gtactggtct gggcttgctt gtaggcggat ttttcctgct
    19981 aaagccagct ccacaaacac ctgtaaagga aacgaatttg caggctgaag ttgcagctgt
    20041 ttccaaggat ttggtatccg aaaaggaagt gaacaaggaa gagaaggaag aaccagttga
    20101 acaagatcta atcacagtag atgtcaaagg tgctgtcaaa tcgccaggga tttatgactt
    20161 gcctgtaggt agtcgagtca atgatgctgt tcagaaggct ggtggcttga cagagcaagc
    20221 agacagcaag tcgctcaatc tagctcagaa agttagtgat gaggctctgg tttacgttcc
    20281 tactaaggga gaagaagcag ttagtcaaca gactggttcg gggacagctt cttcaataag
    20341 caaggaaaag aaggtcaatc tcaacaaggc cagtctggaa gaactcaagc aggtcaaggg
    20401 actgggagga aaacgagctc aggacattat cgaccatcgt gaggcaaatg gcaagttcaa
    20461 gtcagtagac gagctcaaga aggtctctgg cattggtggc aaaacaatag aaaagcttaa
    20521 agactatgtt acagtggatt aagaatttct ctattcccct aatttacctg agttttctat
    20581 tactttggct ttattacgct attttctcag catcttatct tgctttgttg ggctttgttt
    20641 ttctgctagt ctgtctcttt atccaatttc cgtggaaatc tgctggtaaa gttctaataa
    20701 tttgcggaat ctttggattt tggtttgttt ttcaaaattg gcaacagagt caagcgagtc
    20761 aaaatctggc ggattctgtt gaaagggtac ggattttgcc tgatactatt aaggttaatg
    20821 gtgatagtct atcctttcgt ggcaagtcta acggtcgtgc tttccaagtc tattataaac
    20881 tccagtccga ggaggagaaa gaagcctttc aagctttaac cgacctgcat gagataggac
    20941 tagaagggaa gctttcggag ccagaagggc agagaaattt tggtggcttt aattaccaag
    21001 cctatctgaa gactcaggga atttaccaga ctctcaatat caaaacaatc cagtcacttc
    21061 aaaagattgg cagttgggat ataggagaaa acttgtccag tttacgtcga aaggctgtgg
    21121 tttggattaa gacgcacttt ccagacccta tgcgcaatta catgacagga ctcttgctgg
    21181 gacatctgga caccgacttt gaggagatga atgagcttta ttccagtcta ggaattatcc
    21241 acctctttgc cctatctggt atgcaggtag gttttttcat gaatggattt aagaaacttc
    21301 tcttgcgatt gggcttgacc caagaaaagt tgaaatggct gacttatccc ttttccctta
    21361 tctatgcggg actaactgga ttttcagcat cggttattcg cagtctcttg caaaagctac
    21421 tggctcaaca tggggttaag ggcttggata attttgcctt gacggtgctt gtcctcttta
    21481 ttgtcatgcc aaactttttc ttgacagcag gaggagtctt gtcctgcgct tatgctttta
    21541 tcctgaccat gaccagcaaa gaaggggagg ggctcaaggc tgttactagt gaaagtctag
    21601 tcatctcctt gggcatattg cccattctat ccttctattt tgcggaattt caaccttggt
    21661 ctatcctttt gacctttgtc ttttcctttc tatttgactt gaccttctta ccgctcttgt
    21721 ctattttatt tgtcctttcc tttctctatc cagtcattca gctgaacttt atctttgaat
    21781 ggttggaggg cattattcgc ttggtgtcac aggtgacaag tagacctctg gtctttggac
    21841 aacccaatgc atggttttta atcctattgt taatttcctt ggctttggtc tatgatttga
    21901 gaaaaaacat taaaaagcta acggtattgt gcttattgat tacagggctc tttctcctga
    21961 ccaagcatcc actggaaaat gaaatcacca tgctggatgt ggggcaagga gaaagtattt
    22021 tcctacggga tgtaactggg aaaaccattc tcatagatgt aggtggtaag gcagaatctt
    22081 ataagaaaac cgaaaaatgg caagaaaaga tgacgaccag caatgcacag agaagcttga
    22141 taccctatct taaaagtcga ggagtagcta agattgacca gctgattttg acaaatacgg
    22201 acaaggaaca tgtcggagat ttgttagagg tgaccaaggc tttccatgta ggggagattc
    22261 tagtatcaaa aggcagtttg aagcagaagc aatttatggt agaactacag gcgactcaaa
    22321 caaaggtgcg tagtatgata gtaggggaga acttgcccat ttttggaagt cagttagaag
    22381 ttctatctcc aaggaaaatg ggagatggag gacacgatga taccctagtt ctgtatggga
    22441 aattcttgga taagcaattt ctcttcacgg gaaatttgga ggagaaagga gagaaggact
    22501 tgctgaagca ctatccagac ttgaaagtaa atgttttgaa agctagccaa catggcaata
    22561 aaaaatcatc aagtccagcc tttctagaaa aactcaaacc agagcttact cttatctcag
    22621 ttggaaagag caatcgaatg aaactccccc atcaggaaac attgacacga ctggaaggta
    22681 tcaatagcaa agtttatcga actgaccagc aaggagctat acgttttaag gggttggata
    22741 gttggaaaat cgaaagtgtt cgataggaaa gataaatgtt gtagattagt gaaataaact
    22801 aaaaatttgt tgcataataa tgataaaaat ggtataatga aaacgtattc aatattgagg
    22861 atataaaatc attaaaaatc agcaaaagtt attttattag ttagtttata atctattggt
    22921 cttcttcagt ccagtgtatc tgctaagagt gttcttaaaa gtatcggtat gattggaata
    22981 tcttctataa gtctagtatg aatttccatg gatatagaca tactaacgtt ccgtcatggt
    23041 ctcgttatta cagctattct gagtatccag ttggttctgg ctggaactac ggtcgttatg
    23101 agatcataaa ctattacagc ggaggctatt aattcttaaa gagtgagaaa aaggagggct
    23161 agatatgttg cagcttactc atgtgacctt aaaaacgcga caagtcatct tgcaagatgc
    23221 ggatttcacc tttaaaaagg gtaggtttta tggtcttctt gctatcaatg gctctggaaa
    23281 gacgaccctg ttccgtgcca ttagcaattt aattcccata agtagtggaa atatcgcagc
    23341 ccctccttct ttattttatt atgagagtat tgaatggctg gatggaaact taagtgggat
    23401 ggactacctt cgtcttatca aaaacatctg gaagtcagac ctaaacttga gagatgaaat
    23461 cgcctactgg gaaatggctg actatatcag tcttcccatc cgcaagtatt ccttaggcat
    23521 gaagcaacgc ttggtgattg ctatgtattt tctcagtcag gcaaaatgct ggctcatgga
    23581 tgagattaca aatggcttag acgagtatta tcgacagaag ttttttgata ggctagcaca
    23641 aatcgataga caagaacagc tggttctttt aagttcccac tataaggaag agttggttga
    23701 tgtctgcgat agagtagtaa ccattcatca ggggcagata gaagaggttt agtttatgaa
    23761 agatgttagt ctatttttat tgaaaaaagt tttcaaaagc cgcttaaact ggattgtctt
    23821 agctttattt gtatctgtac tcggtgttac cttttattta aatagtcaga ctgcaaactc
    23881 acacagcttg gagagcaggt tggaaagtcg cattgcagcc aacgagaggg ctatcaatga
    23941 aaatgaagag aaactctccc aaatgtctga taccagctcg gaggaatacc agtttgctaa
    24001 aaataattta gacgtgcaaa aaaatctttt gacgcgaaag acagaaattc taactttact
    24061 aaaagaaggg cgctggaaag aagcctacta tctgcagtgg caagatgaag agaagaatta
    24121 tgaatttgta tcaaatgacc cgactgctag ctctggctta aaaatggggg ttgaccgcga
    24181 acggaagatt taccaagccc tgtatccctt gaacataaaa gcacatactt tggagtttcc
    24241 gacccacggg attgatcaga ttgtctggat tttagaggtt atcatcccaa gtttgtttgt
    24301 ggttgctatt atttttatgc taacacaact atttgcagaa agatatcaaa atcatctgga
    24361 cacagctcac ttatatcctg tttcaaaagt gacatttgca atatcctctc ttggagttgg
    24421 agtgggatat gtaactgtgc tgtttatcgg aatctgtggc ttttcttttc tagtgggaag
    24481 tctgataagt ggttttggac agttagatta tccctaccca atttatagct tagtgaatca
    24541 agaagtaact attgggaaaa tacaagatgt attatttcct ggcttgctct tagctttctt
    24601 agcctttatc gtcattgtgg aagttgtgta cttgattgct tactttttca agcaaaaaat
    24661 gcctatcctc tttctttcac tcattgggat tgttggctta ttgtttggta tccaaaccat
    24721 tcagcctctt caaaagattg cacatctgat tccctttact tacttgcgtt cagtggagat
    24781 tttatctgga agattaccta agcaaattga taatgtcaat ctaaattgga gcatggggat
    24841 ggtcttgctt ccttgcctga ttatcttttt gctattggga attctattta ttgaaagatg
    24901 gggaagttca cagaaaaaag aattttttaa tagattctag ctttcctata ggtagggaaa
    24961 ataagtaaaa actaacatag agagggaatc aacttgattc tctctttttg attcgaaaac
    25021 caaaccaaaa tacaaacaca aacttttcaa aaaataactt tttatcttga caagagctag
    25081 aaaacttggt atcatataaa agttgagaaa agcagaagtg agagcttctc gccttgtgac
    25141 attaagttgc ctggccctac ggatgaaaag tttcgaagaa acgctatcat aacgtgcggg
    25201 cttgtatatt tacgagtccg ctattgtttt tctctaataa aacaaaagag gtgaaaacca
    25261 tagcaaagca agacttattc atcaatgatg agattcgtgt acgtgaagtt cgcttgattg
    25321 gtcttgaggg agaacagcta ggcatcaagc cactcagtga agcgcaagct ttggctgata
    25381 acgctaatgt tgacctagta ttgattcaac cccaagccaa accgcctgtt gcaaaaatta
    25441 tggactacgg taagttcaaa tttgagtacc agaagaagca aaaagaacaa cgtaaaaaac
    25501 aaagcgttgt tactgtgaaa gaagttcgtc taagtccaac aattgacaag ggtgactttg
    25561 atacaaaact tcgcaatgca cgtaagttcc ttgaaaaagg aaataaagtt aaggtatcta
    25621 ttcgctttaa gggtcgtatg attacccata aagagattgg tgcaaaagtt ttagccgagt
    25681 ttgctgaagc aactcaagat attgccatca tcgaacaacg tgctaaaatg gatggacgtc
    25741 aaatgttcat gcaattggcg ccagcgactg acaaaaaata attgtcagaa agttaaataa
    25801 aggagaaaaa atcatgccaa aacaaaaaac acaccgcgca tcagctaaac gtttcaaacg
    25861 tacaggttct ggtggactta aacgtttccg tgcttacact tctcaccgtt tccacggaaa
    25921 aactaagaaa caacgtcgtc atcttcgtaa agcatctatg gtgcattcag gagattacaa
    25981 acgtatcaaa gcaatgctta ctcgcttgaa ataatagctt gactgtaagt aaacaattct
    26041 aggaaatatt tggaggaaat aaaacatggc acgtgttaaa ggtggcgttg tatcacgcaa
    26101 acgtcgtaaa cgtattctta aattagcaaa aggttactat ggagctaaac acatcttgtt
    26161 ccgtactgca aaagaacaag taatgaactc ttactactat gcataccgtg accgtcgtca
    26221 gaaaaaacgt gacttccgca aattgtggat tactcgtatc aatgcggcag ctcgtatgaa
    26281 cggactttca tactcacaat tgatgcatgg tttgaaattg gctgagattg aagttaaccg
    26341 taaaatgctt gctgacttgg ctgttaacga tgcagtagct ttcacagctc ttgcagatgc
    26401 agctaaagca aaacttggta aataacaact gatgagactg gaacaggatt gtccagtctt
    26461 tttaaaaata aaggagaaag atatggcttc aaaaatgcta cacacttgct tacgagtaga
    26521 aaatcttgaa aaatcaatcg cattttatca agatgctttt ggttttaaag aattgcgtcg
    26581 cagagatttt ccagaccatg ccttcacgat tgtctatcta ggtcttgagg gtgatgatta
    26641 tgagttggag ttgacttata actacgatca cggtccgtat gtggttggtg atgggtttgc
    26701 ccatatcgcc ctcagtacac ctgatcttga ggcacttcat caagaacaca gtgcaaaagg
    26761 ctatgaagtg actgagccaa atggtctacc aggaactaca ccaaactatt actttgttaa
    26821 agaccctgat ggatataagg tcgaagtcat tcgtgaaaaa taatttcgtg caagttaagt
    26881 agaatattcg ttttctactt gacttttttt agctgaaaga gtatactaat aaaaattcaa
    26941 cctttaaggg gagtccagag agactcacaa ggtgtcagat aaaagaatag tgcaattttc
    27001 tagaggagac cttttgagtg tgctctcttg tgttgtacga ttttaactga ggccttgcac
    27061 tagcaaggtc ttttctttat ctggtcccct taaaatttaa ggaggaaaag ttatgaatct
    27121 cacatgtaag aagcgtttgg gtgtcattcg gttggaaacc atgaaggtgg ttgcacaaga
    27181 ggaaatcgcg ccagcaatct ttgaattagt cctagaagga gaaatggttg aagccatgcg
    27241 agcaggccaa tttcttcatc tgcgtgtacc agacgatgcc catctcttac gtcgtcctat
    27301 ttcaatttcg tctattgaca aggcaaacaa gcagtgtcac ctcatttatc ggattgaggg
    27361 agctgggact gcaatttttt caaccttaag tcagggagac actcttgatg tgatggggcc
    27421 tcagggaaat ggttttgact tgtctgacct tgatgagcag aatcaggttc tccttgttgg
    27481 tggtgggatt ggtgttccac ccttgcttga ggtggccaag gaattgcatg aacgtggagt
    27541 gaaagtagtg acagtcctcg gttttgctaa taaggatgct gttattttga aaacggaatt
    27601 ggctcagtat ggtcaggtct ttgtaacgac agatgatggt tcttatggca tcaagggaaa
    27661 tgtttccgtt gttatcaatg atttagatag tcagtttgat gctgtttact cgtgtggggc
    27721 tccaggaatg atgaagtata tcaatcaaac ctttgatgat cacccaagag cctatttatc
    27781 tctggaatct cgtatggctt gtgggatggg agcttgctat gcctgtgttc taaaagtacc
    27841 agaaaacgag acggtcagcc aacgcgtctg tgaagatggt cctgttttcc gcacaggaac
    27901 agttgtatta taaggagaaa attatgacta caaatcgatt acaagtttct ctacctggtt
    27961 tggatttgaa aaatccgatt attccagcat caggctgttt tggctttgga caagagtatg
    28021 ccaagtacta tgatttagac cttttaggtt ctattatgat caaggcgaca acccttgaac
    28081 cacgttttgg gaatccaact ccaagagtgg cagagacgcc tgctggtatg ctcaatgcaa
    28141 ttggcttgca aaatcctggt ttagaggttg ttttggctga aaagctacct tggctggaaa
    28201 gagaatatcc aaatcttcct attattgcca atgtagctgg tttttcaaaa caagagtatg
    28261 cagctgtttc tcatgggatt tccaaggcaa ctaatgtaaa agctatcgag ctcaatattt
    28321 cttgtcccaa tgttgaccac tgtaatcatg gacttttgat tggtcaagat ccagatttgg
    28381 cttatgatgt ggtgaaagca gctgtggaag cctcagaagt gccagtttat gtcaaattaa
    28441 ccccgagtgt gaccgatatc gttactgtcg caaaagctgc agaagatgcg ggagcaagtg
    28501 gcttgaccat gatcaatact ctggttggaa tgcgctttga cctcaaaacc agaaaaccaa
    28561 tcttggccaa tggaacaggt ggaatgtcag gtccagcagt ctttccagta gccctcaaac
    28621 tcatccgcca agtagcccaa acaacagacc tgcctatcat tggaatgggg ggagtggatt
    28681 cgactgaagc tgccctagaa atgtatctag ctggggcatc tgctatcgga gttggaacag
    28741 ctaactttac caatccttat gcctgccctg acatcatcga aaatttacca aaagtcatgg
    28801 ataaatacgg tattagcagt ctggaagaac tccgtcagga agtaaaagag tctctgaggt
    28861 aaactgcaat caatctgttc ttgatttttt attagtttgt aatatgaatt taggagaatt
    28921 ttggtacaat aaaataaata agaacagagg aagaaggtta atgaagaaag taagatttat
    28981 ttttttagct ctgctatttt tcttagctag tccagagggt gcaatggcta gtgatggtac
    29041 ttggcaagga aaacagtatc tgaaagaaga tggcagtcaa gcagcgaatg agtgggtttt
    29101 tgatactcat tatcaatctt ggttctatat aaaagcagat gctaactatg ctgaaaatga
    29161 atggctaaag caaggtgacg actattttta cctcaaatct ggtggctata tggccaaatc
    29221 agaatgggta gaagacaagg gagcctttta ttatcttgac caagatggaa agatgaaaag
    29281 aaatgcttgg gtaggaactt cctatgttgg tgcaacaggt gccaaagtaa tagaagactg
    29341 ggtctatgat tctcaatacg atgcttggtt ttatatcaaa gcagatggac agcacgcaga
    29401 gaaagaatgg ctccaaatta aagggaagga ctattatttc aaatccggtg gttatctact
    29461 gacaagtcag tggattaatc aagcttatgt gaatgctagt ggtgccaaag tacagcaagg
    29521 ttggcttttt gacaaacaat accaatcttg gttttacatc aaagaaaatg gaaactatgc
    29581 tgataaagaa tggattttcg agaatggtca ctattattat ctaaaatccg gtggctacat
    29641 ggcagccaat gaatggattt gggataagga atcttggttt tatctcaaat ttgatgggaa
    29701 aatagctgaa aaagaatggg tctacgattc tcatagtcaa gcttggtact acttcaaatc
    29761 cggtggctac atggcagcca atgaatggat ttgggataag gaatcttggt tttacctcaa
    29821 gtttgatggc aaaatggctg aaaaagaatg ggtttacgat tctcatagtc aagcctggta
    29881 ctacttcaaa tccggtggtt acatgacagc caatgaatgg atttgggata aggaatcttg
    29941 gttttacctc aaatctgatg ggaaaatagc tgaaaaagaa tgggtctaca attctcatag
    30001 tcaagcttgg tactacttca aatccggtgg ttacatgaca gccaatgaat ggatttggga
    30061 taaggaatct tggttttacc tcaagtctga tggcaaaatg gctgaaaaag aatgggtcta
    30121 cgattctcat agtcaagcct ggtactactt caaatctggt ggctacatgg cgaaaaatga
    30181 gacagtagat ggttatcagc ttggaagcga tggtaaatgg cttggaggaa aagctacaaa
    30241 tgaaaatgct gcttactatc aagtagtgcc tgttacagcc aatgtttatg attcagatgg
    30301 tgaaaagctt tcctatatat cgcaaggtag tgtcgtatgg ctagataagg atagaaaaag
    30361 tgatgacaag cgcttggcta ttactatttc tggtttgtca ggctatatga aaacagaaga
    30421 tttacaagcg ctagatgcta gtaaggactt tatcccttat tatgagagtg atggccaccg
    30481 tttttatcac tatgtggctc agaatgctag tatcccagta gcttttcatc tttctgatat
    30541 ggaagtaggc aagaaatatt attcggcaga tggcctgcat tttgatggtt ttaagcttga
    30601 gaatcccttc cttttcaaag atttaacaga ggctacaaac tacagtgctg aagaattgga
    30661 taaggtattt agtttgctaa acattaacaa tagccttttg gagaacaagg gcgctacttt
    30721 taaggaagcc gaagaacatt accatatcaa tgctctttat ctccttgccc atagtgccct
    30781 agaaagtaac tggggaagaa gtaaaattgc caaagataag aataatttct ttggcattac
    30841 agcctatgat acgacccctt acctttctgc taagacattt gatgatgtgg ataagggaat
    30901 tttaggtgca accaagtgga ttaaggaaaa ttatatcgat aggggaagaa cttttcttgg
    30961 aaacaaggct tctggtatga atgtggaata tgcttcagac ccttattggg gcgaaaaaat
    31021 tgctagtgtg atgatgaaaa tcaatgagaa gctaggtggc aaggattagt actataagtg
    31081 aatatgattt gagtgaatag taagttaaaa atcctgattt caagtaaaat caggattttt
    31141 tcatggatgc aatttttttg gagtctggtg tgacgcggag ggtcttttgt cctgtgtaag
    31201 tgacaaagcc gggttttcca ccagttggtt tattgagttt tttgacttca atcatatcta
    31261 cctgcaccag attcgacagg cgcccttgag agaagtaggc agctaactct gctgcgtctg
    31321 tcttgactgc atcagatggg tcaagatttc ctgagatgac aacatggctt ccaggaatgt
    31381 ccttagcatg gaaccaaagt tcctccttgc gggccatttt aaaggtcaat tcctcatttt
    31441 gaagattgtt tcgtccgaca tagatgatgg ttttgccatc gcttgctaga tattgttcta
    31501 gttttttgcg tttctggatt ttctcccgtt gtcttctgcg gataaaacct gtttgaatca
    31561 attcttcacg gatttcagcg atttcttcca gtccagcttg gttgaggacg gtttctacac
    31621 tttccagata gagaatagtg gctttggttt cttcaatcaa atcagtcaag tatttgacag
    31681 cttctttgag tttctgatac cgtttaaaat agcgttgggc attctggttg ggagtcagag
    31741 ccttatcaag cgcaatcatg ataggttggt tggtatagta gttgtctagg ataacctggt
    31801 cttggtcgtt aggcacttgg tgtaggaagg ttgtcagcaa ttctcctttt tgacgaaatt
    31861 cttcagcgtt gtctgtcgcc agtaactctt tttcctgttt tttgagtttg tgtcggtttt
    31921 tctgaagttc attttcaaca cgacgaatca gttcactggc ctgctgtttg acgcggtcgc
    31981 gctcagcctt atccttatag taggtgtcca acaaatcaga aagatttgca aaaggctctc
    32041 ccacctgatt tgcaaaagga actggactga aggaagtctc agtcaagcat ggcttggttt
    32101 cttgattgaa aaaatttcgg aaagcggaaa gtttttcact aaccagtatc ctttccaatt
    32161 catttgccgt atcgcgtccc agaccttgaa agaggctttg aagatttttt gctgttagtt
    32221 cttgggtttg caggatttca aagagctttt catccttgat agtaaaagga ttgagagatt
    32281 ctgtacttgg cggagcgata taggtcgatc ctggaagtaa ggtgcggtag ctattttgtg
    32341 aaaaaccgac gtgtttgata acttcgagga ttttatgact gcttttatcg accagtagaa
    32401 tattactgtg tttccccata atttcaataa tcaaggtagc ctggatatgg tctccaatct
    32461 cgtttttatt ggaaactgtc atttccacaa tacggtcatt ttccacttgc tcaatcgact
    32521 caatcagggc cccctgcaaa tactttctca aaaccatgat aaaggtagaa ggttgagctg
    32581 gattttcaaa agtcgtttgg gtcagctgaa tgcgtccaaa aactggatgg gcagaaagga
    32641 gcaggcgatg gctttggcga ttgctgcgga tttgcaagac caactcttgt tcaaaaggct
    32701 gattgatttt ctggatgcga ccattcacta attcgtttcg caattcctca actatgtggt
    32761 gtaaaaaaaa tccatcaaat gacatcgttc tctccttgtg attgtattcc atagtattat
    32821 atcaaaaagg tagaataaaa tcatggaaat gtggtataat aaagccaagt aaagagaaat
    32881 gagaagcaca tgtatattga aatggtagat gaaactggtc aagtttcaaa agaaatgttg
    32941 caacaaaccc aagaaatttt ggaatttgca gcccaaaaat taggaaaaga agacaaggag
    33001 atggcagtca cttttgtgac caatgagcgt agtcatgaac ttaatctgga gtaccgtgac
    33061 accgaccgtc cgacagatgt catcagcctt gagtataaac cagaattgga aattgccttt
    33121 gacgaagagg atttgcttga aaatccagaa ttggcagaga tgatgtctga gtttgatgcc
    33181 tatattgggg aattgttcat ctctatcgat aaggctcatg agcaggccga agaatatggt
    33241 cacagctttg agcgtgagat gggcttcttg gcagtacacg gctttttaca tattaacggc
    33301 tatgatcact acactccgga agaagaagcg gagatgttcg gtttacaaga agaaattttg
    33361 acagcctatg gactcacaag acaataaacg aaaatggaaa aatcgtgacc tgatatccag
    33421 tttagaattt gccatcacag ggatttttac tgctatcaag gaagaacgaa atatgcgaaa
    33481 acatgcagtg acggctctag tggtcatcct tgcaggtttt gtttttcagg tgtcacgaat
    33541 cgaatggctc tttctcctat tgagcatttt cttggtagta gcctttgaga ttatcaactc
    33601 tgctattgaa aatatggtgg atttggccag tcactatcac ttttccatgc tggctaaaaa
    33661 tgccaaggat atggcggctg gcgcggtatt agtggtttct cttttcgcag ccttaacagg
    33721 tgcattgatt tttctcccac gaatctggga tttattattt taaacagtaa gaggaaatta
    33781 tgacttttaa atcaggcttt gtagccattt taggacgtcc caatgttggg aagtcaacct
    33841 ttttaaatca cgttatgggg caaaagattg ccatcatgag tgacaaggcg cagacaacgc
    33901 gcaataaaat catgggaatt tacacgactg ataaggagca aattgtcttt atcgacacac
    33961 cagggattca caaacctaaa acagctctcg gagatttcat ggttgagtct gcctacagta
    34021 cccttcgcga agtggacact gttcttttca tggtgcctgc tgatgaagcg cgtggtaagg
    34081 gggacgatat gattattgag cgtctcaagg ctgccaaggt tcctgtgatt ttggtggtga
    34141 ataaaatcga taaggttcat ccagaccagc tcttgtctca gattgatgac ttccgtaatc
    34201 aaatggactt taaggaaatt gttccaatct cagcccttca gggaaataac gtgtctcgtc
    34261 tagtggatat tttgagtgaa aatctggatg aaggtttcca atatttcccg tctgatcaaa
    34321 tcacagacca tccagaacgt ttcttggttt cagaaatggt tcgcgagaaa gtcttgcatc
    34381 taactcgtga agagattccg cattctgtag cagtagttgt tgactctatg aaacgagacg
    34441 aagagacaga caaggttcac atccgtgcaa ccatcatggt cgagcgcgat agccaaaaag
    34501 ggattatcat cggtaaaggt ggcgctatgc ttaagaaaat cggtagcatg gcccgtcgtg
    34561 atatcgaact catgctagga gacaaggtct tcctagaaac ctgggtcaag gtcaagaaaa
    34621 actggcgcga taaaaagcta gatttggctg actttggcta taatgaaaga gaatactaag
    34681 tagaggtagg ctcatgcctg cttcttgttt ttacagaagg aggacttatg cctgaattac
    34741 ctgaggttga aaccgtttgt cgtggcttag aaaaattgat tataggaaag aagatttcga
    34801 gtatagaaat tcgctacccc aagatgatta agacggattt ggaagagttt caaagggaat
    34861 tgcctagtca gattatcgag tcaatgggac gtcgtggaaa atatttgctt ttttatctga
    34921 cagacaaggt cttgatttcc catttgcgga tggagggcaa gtatttttac tatccagacc
    34981 aaggacctga acgcaagcat gcccatgttt tctttcattt tgaagatggt ggcacgcttg
    35041 tttatgagga tgttcgcaag tttggaacca tggaactctt ggtgcctgac cttttagacg
    35101 cctactttat ttctaaaaaa ttaggtcctg aaccaagcga acaagacttt gatttacagg
    35161 tctttcaatc tgcccttgcc aagtccaaaa agcctatcaa atcccatctc ctagaccaga
    35221 ccttggtagc tggacttggc aatatctatg tggatgaggt tctctggcga gctcaggttc
    35281 atccagctag accttcccag actttgacag cagaagaagc gactgccatt catgaccaga
    35341 ccattgctgt tttgggccag gctgttgaaa aaggtggctc caccattcgg acttatacca
    35401 atgcctttgg ggaagatgga agcatgcagg actttcatca ggtctatgat aagactggtc
    35461 aagaatgtgt acgctgtggt accatcattg agaaaattca actaggcgga cgaggcaccc
    35521 acttttgtcc aaactgtcaa aggagggact gatgagaaaa atcatcggaa tcactggggg
    35581 aattgcctct ggtaagtcaa ctgtgacaaa ttttctaaga cagcaaggct ttcaagtagt
    35641 ggatgccgac gcagtcgtcc accaactaca gaaatctggt ggtcgtctgt ttgaggctct
    35701 agtacagcac tttgggcaag aaatcattct tgaaaacgga gaactcaatc gccctctcct
    35761 agctagtctc atcttttcaa atcctgaaga gcaaaaatgg tctaagcaaa ttcaagggga
    35821 gattatccgt gaggaactgg ctactttgag agaacagttg gctcagacag aagagatttt
    35881 cttcatggat attcccctac tttttgagca ggactacagc gattggtttg ctgagacttg
    35941 gttggtctat gtggaccgag atacccaagt agaacgctta atgaaaaggg accagttgtc
    36001 caaagatgaa gctgagtctc gtctggcagc ccagtggcct ttagaaaaaa agaaagattt
    36061 ggccagccag gttcttgata ataatggcaa tcagaaccag cttcttaatc aagtgcatat
    36121 ccttcttgag ggaggtaggc aagatgacag agattaactg gaaggataat ctgcgcattg
    36181 cctggtttgg taattttctg acaggagcca gtatttcttt ggttgtacct tttatgccca
    36241 tcttcgtgga aaatctaggt gtagggagtg agcaagtcgc tttttatgca ggcttagcaa
    36301 tttctgtctc tgctatttcc gcggcgctct tttctcctat ttggggtatt cttgctgaca
    36361 aatacggccg aaaacccatg atgattcggg caggtcttgc tatgactatc actatgggag
    36421 gcttggcctt tgtcccaaat atctattggt taatctttct tcgtttacta aacggtgtat
    36481 ttgcaggttt tgttcctaat gcaacggcac tgatagccag tcaggttcca aaggagaaat
    36541 caggctctgc cttaggtact ttgtctacag gcgtagttgc aggtactcta actggtccct
    36601 ttattggtgg ctttattgca gaattatttg gcattcgtac agttttctta ctggttggta
    36661 gttttctatt tttagctgct attttgacta tttgctttat caaggaagat tttcaaccag
    36721 tagccaagga aaaggccatt ccaacaaagg aattatttac ctcggttaaa tatccctatc
    36781 ttttgctcaa tctcttttta accagttttg tcatccaatt ttcagctcaa tcgatcggcc
    36841 ctattttggc tctttatgta cgcgacttag ggcagacaga gaatcttctt tttgtctctg
    36901 gtttgattgt gtccagtatg ggcttttcca gtatgatgag tgcaggagtt atgggaaagc
    36961 taggtgacaa ggtgggcaat catcgtctct tggttgtcgc ccaattttat tcagtcatca
    37021 tctatctcct ctgtgccaat gcctctagcc cccttcaact aggactctat cgattcctct
    37081 ttggcttggg aaccggtgcc ttgattcccg gggttaatgc cctactcagc aaaatgactc
    37141 ccaaagccgg catttcgagg gtctttgcct tcaatcaggt attcttttat ctgggaggtg
    37201 ttgttggtcc catggcaggt tctgcagtag caggtcaatt tggctaccat gctgtctttt
    37261 atgcgacaag cctttgtgtt gcctttagtt gtctctttaa cctgattcaa tttcgaacat
    37321 tattaaaagt aaaggaaatc tagtgcgagt aaaaattaat ctcaaatgct cctcttgtga
    37381 cagtatcaat tacctaacca gtaaaaattc aaaaacccat ccagacaaga ttgaggtttt
    37441 aaagtattgt cccaaggaaa gaaaagtaac cctacatctt gaatctaagt agattttatg
    37501 gtaaaataat agggatttta aggagtttga tatgtataac ctattattaa ccattttatt
    37561 agtattatct gttgtgattg tgattgcaat tttcatgcaa ccaaccaaaa accaatccag
    37621 caatgtattt gatgccagtt caggtgattt gtttgaacgc agtaaagctc gcggttttga
    37681 agctgtaatg cagcgtttga cagggatttt agtctttttc tggctagcca ttgccttagc
    37741 attgacggta ttatcaagta gataagaaaa taatgggcag gactaggtct ttgcctcttt
    37801 ttatttttaa aggatgtttg agaaggtttt acagtaaaag aaaattaaaa aatctagaaa
    37861 gaaaatatga aagatagaat aaaagaatat ttacaagaca agggaaaggt gactgttaat
    37921 gatttggctc aggctttggg aaaagacagt tccaaggatt ttcgtgagtt gattaaaacc
    37981 ttgtccttaa tggaaagaaa gcaccaaatt cgttttgaag aagatggcag tctgaccttg
    38041 gaagccaaga aaaaacatga gattaccctc aaggggattt ttcatgccca taaaaatggt
    38101 tttggttttg tcagcctaga aggggaagag gacgaccttt ttgtagggaa aaatgatgtc
    38161 aactatgcta ttgatggtga taccgtcgag gtagtgatta agaaagtcgc tgaccgcaat
    38221 aagggaacag cagcagaagc caaaattatt gatatcctag aacacagttt gacaacagtt
    38281 gtcgggcaaa tcgttctgga tcaggaaaag cctaagtatg ctggctatat tcgttcaaaa
    38341 aatcagaaaa tcagtcaacc gatttatgtt aagaaaccag ccctaaagtt agaaggaaca
    38401 gaagttctca aggtctttat cgataaatac ccaagcaaga aacatgattt ctttgtcgcg
    38461 agtgttctcg atgtggtggg acactcaacg gatgtcggaa ttgatgttct tgaggtcttg
    38521 gaatcaatgg acattgtatc cgagtttcca gaagctgttg tcaaggaagc agaaagtgtg
    38581 cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg atctaagaga tgaaattacc
    38641 tttaccattg acggtgcgga tgccaaggac ttggacgatg cagtgcatat caaggctctg
    38701 aaaaatggca atctggagct tggggttcac atcgcagatg tttcctatta tgtgaccgag
    38761 ggttctgccc ttgacaagga agcccttaac cgtgcgactt ctgtttacgt gacagaccga
    38821 gtggtgccaa tgcttccaga acgactatca aatggcatct gctctctcaa tccccaagtt
    38881 gaccgcctga cccagtctgc tattatggag attgataaac atggtcgtgt ggtcaactat
    38941 accattacac aaacagttat caagaccagt tttcgtatga cctatagcga tgtcaatgat
    39001 atcctagctg gcgatgaaga aaagagaaaa gaataccata aaattgtacc aagtatcgaa
    39061 ctcatggcca agcttcatga aactttagaa aacatgcgtg tgaaacgtgg agctctcaat
    39121 tttgatacca atgaagcgaa gattttagtg gataaacaag gtaagcctgt tgatatcgtt
    39181 cttcggcagc gtggcattgc cgagcggatg attgagtctt ttatgttgat ggctaatgaa
    39241 acagttgccg aacatttcag caagttggat ttgcctttta tctaccgaat tcacgaggag
    39301 cctaaggctg aaaaggttca gaagtttatt gattatgctt cgagttttgg cttgcgcatt
    39361 tatgggactg ccagtgagat tagccaggag gcacttcaag acatcatgcg tgctgttgag
    39421 ggagaacctt atgcagatgt attgtccatg atgcttcttc gctctatgca gcaggctcgt
    39481 tattcggagc acaatcacgg ccactatgga ctagctgctg actattatac tcactttacc
    39541 agtccaattc gtcgttatcc agaccttctt gttcaccgta tgattcggga ttacggccgt
    39601 tctaaggaaa tagcagagca ttttgaacaa gtgattccag agattgcgac ccagtcttcc
    39661 aaccgtgaac gtcgtgccat agaagctgag cgtgaagtcg aagccatgaa aaaggctgag
    39721 tatatggaag aatacgtggg tgaagagtat gatgcagttg tatcaagtat tgtcaaattc
    39781 ggtctctttg tcgaattgcc aaacacagtt gaaggcttga ttcacatcac taatctacct
    39841 gaattttatc atttcaatga gcgtgatttg actcttcgtg gagaaaaatc aggtatcact
    39901 ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag cggataaaat gactggagag
    39961 attgattttt cattcgtacc tagtgagttt gatgtgattg aaaaaggctt gaaacagtct
    40021 agtcgtagtg gcagagggcg tggttcaaat cgtcgttcgg ataagaagga agacaagaga
    40081 aaatcaggac gctcaaatga taagcgtaaa cattcacaaa aagacaagaa gaaaaaagga
    40141 aagaaacctt tttacaagga agtagctaag aaaggagcca agcatggcaa agggcgaggg
    40201 aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat acaatcgtag atacgctaga
    40261 ggcagggatg gtcctgactg gaactgaaat caagagtgta cgagctgctc gaattaatct
    40321 caaggatggc tttgctcaag tgaaaaatgg agaagtttgg ctgagcaatg ttcatatcgc
    40381 accttacgaa gagggcaata tctggaacca ggaaccagaa cgtcgtcgta aactcctgct
    40441 ccataaaaag caaattcaaa aattggaaca agagaccaaa gggacaggaa tgaccttagt
    40501 tccccttaag gtctatataa aagatggcta cgctaagctt cttttaggac ttgccaaagg
    40561 gaagcatgac tatgacaaac gggagtctat caaacgtcgt gagcaaaatc gagatatcgc
    40621 gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa ttgaaaatgg aaaaattagt
    40681 tgcctataaa cgcatgcctt tgtggaataa acaaacaatg cctgaagctg ttcagcaaaa
    40741 gcacaataca aaagttggga cttgggggaa aattactgtc ttgaagggag ctctcaagtt
    40801 tattgaattg acagaagaag gggaagttct agctgaacac ctctttgaag caggggcaga
    40861 caatccaatg gcccagcctc aagcctggca ccgagtggaa gctgccacag atgatgtgga
    40921 atggtacttg gaattttatt gtaaacctga ggattatttt gctaaaaaat acaataccaa
    40981 acctgttcat tcagaggtcc tagaggccat gcagacagtg aaacaaggga aagctttgga
    41041 tttgggttgt ggtcaggggc gtaattctct ttttctagcc cagcaagatt ttgatgtgac
    41101 ggctgtagat caaaatggac tggctcttga aatcttgcaa agcattgtgg agcaggaaga
    41161 tttggacata cctgttgacc tttacgatat caattcagct agcattggac aagaatatga
    41221 ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg gaccgcattc cagctattat
    41281 tcaaaatatg caggagaaaa ccagtgttgg tggttacaac cttatcgttt gtgccatgga
    41341 cacggaggac tatccttgct cggttaactt cccattcacc tttaaagaag gagaactggc
    41401 agactattac aaggattggg aattggttaa gtacaatgaa aatccaggcc atttgcatcg
    41461 tcgcgatgag aatggcaatc gtattcaact acgctttgcg accttactag ctaagaaaat
    41521 caagtaaaca cacatgaaga ttaggaattt tcctgatctt ttttcttttt tacgaatgat
    41581 atagaaaagg agggaattca tgtttgttgc gagagatgct aggggagaat tggtaaatgt
    41641 gttagaggat aaacttgaga agcaagcata cacctgccca gcttgtggag gccagctcca
    41701 tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat aaatccttaa aagactgtga
    41761 ttttttcttt gaaaatgaaa gtccagaaca cctggccaat aaggaatccc tctatcactg
    41821 gttaaaaaaa gagacaaagg ttcaattaga gtacccgctt tcagaactta aacagattgc
    41881 ggatgtattt gtaaatggca atctagctct agaagttcag tgtagtccct tgcctcagaa
    41941 agtccttaaa gagcgaagtg agggatatcg tagtcagggt taccaagtac tgtggttgct
    42001 gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta cagcaaggtt ttctttattt
    42061 cagtcaaaac atgggctttt atgtttggga attagacaag gaaaaacaag ttttaagact
    42121 caaatacctg atttaccagg atctccgcgg taaactccat tatcaaatca aggaattttc
    42181 ctatggtcaa ggtagtttat tggaaatatt gcgtcttccc tataagagac aaaaaatatc
    42241 tcattttaca gtttctgagg acaaggacat ctgtcgctat atccggcaac aactttatta
    42301 tcaaaatctc ttttggatga aagaacaagc agaagcctat caaaagggag aaaatatcct
    42361 gacttatgga ctgaaagaat ggtatccaca aattcgacca atagtgggca aatttttcca
    42421 gattgaacaa gacttgacta gctattatca gcacttttat acctattacc aaaaaaatcc
    42481 tcaaaatgat tggcaaaagc tttatccacc agccttttat cagcaatatt tcttgaaaaa
    42541 tatggtagaa tagaaaggat ggaggaatct aatggtatta caaagaaatg aaagaaatga
    42601 aataaatgaa aaagatacat gggatctatc aacgatctac ccaactgacc aggcttggga
    42661 agaagcctta aaagatttaa cagaacaatt ggagacagta gcccagtatg aaggccatct
    42721 cttggatagt gcggataacc tactagaaat cactgaattt tctcttgaaa tggaacgcca
    42781 gatggagaag ctttacgttt atgctcatat gaagaatgac caggatacac gtgaagctaa
    42841 gtatcaagag tactatgcca aggccatgac actctacagc cagttagacc aagccttttc
    42901 attctatgat cctgaattta tggagattag cgaaaagcag tatgctgact ttttagaagc
    42961 tcaaccaaag ctgcaggttt atcaacacta ttttgacaag ctcttgcaag gcaaggatca
    43021 cgttctttca caacgtgaag aagaattatt ggctggagct ggagaaatct ttggttcagc
    43081 aagtgaaacc ttcgctatct tggacaatgc ggatattgtg ttcccttatg tcctagacga
    43141 tgatggtaaa gaagttcagc tatctcatgg gacttacaca cgtttgatgg agtctaaaaa
    43201 acgtgaggtt cgccgtggtg cctatcaagc tctttatgcg acttacgaac aattccaaca
    43261 cacctatgcc aaaaccttgc aaaccaatgt taaggtgcaa aattaccgtg ctaaagttcg
    43321 taactacaag agtgctcgtc atgcagccct cgcagcgaat tttgttccag aaagtgttta
    43381 tgacaatttg gtagcagcag ttcgcaagca tttgccactc ttacatcgct atcttgagct
    43441 tcgttcaaaa atcttgggga tttcagatct caagatgtac gatgtctaca caccgctttc
    43501 atctgttgaa tacaatttta cctaccaaga agccttgaaa aaagcagaag atgctttggc
    43561 agtcttgggt gaggattact tgagccgtgt caaacgtgcc ttcagcgagc gttggattga
    43621 tgtttacgaa aatcaaggca agcgttcagg tgcctactct ggtggttctt acgataccaa
    43681 tgcctttatg cttctcaact ggcaggacaa tctggacaat ctctttactc ttgttcatga
    43741 aacaggtcac agtatgcatt caagctatac tcgtgaaact cagccttatg tttacggaga
    43801 ttactctatc tttttggctg agattgcctc aactaccaat gaaaatatct tgacggagaa
    43861 attattggaa gaagtggaag acgacgcaac acgctttgct attctcaata acttcctaga
    43921 tggtttccgt ggaacagttt tccgccaaac tcaatttgct gagtttgaac acgccattca
    43981 ccaagcagat caaaatgggg aggtcttgac aagcgatttc ctaaataaac tctacgcaga
    44041 cttgaaccaa gagtattatg gtttgagtaa ggaagacaat cctgaaatcc aatacgagtg
    44101 ggctcgcatt ccacacttct actataacta ctatgtatat caatattcaa ctggctttgc
    44161 ggccgcctca gccttggctg aaaaaattgt ccatggtagt caagaagacc gtgaccgcta
    44221 tatcgactac ctcaaggcag gtaagtcgga ctatccactt aatgtcatga gaaaagctgg
    44281 tgttgatatg gagaaggaag actacctcaa cgatgccttt gcagtctttg aacgccgttt
    44341 aaatgagttt gaagcccttg ttgaaaaatt aggattggca taaaatggtt gaatcgtata
    44401 gtaagaatgc taaccataac atgcgtcgtc ctgtcgtcaa agaagaaatt gtagacttga
    44461 tgcgtcagcg tcaaaagcag gtcacaggtt tcttgaaaga attggaagac tttgcccgca
    44521 aggaaaatat tcctattatt ccccatgaaa cggttgctta tttccgtttt cttatggaaa
    44581 ccatgcagcc taaaaatatt ctggaaattg ggacggctat cggtttttca gctctcttga
    44641 tggctgaaca tgcgccaaat gctaagatta caactattga tcgtaatcca gaaatgattg
    44701 gttttgccaa ggaaaatttt gcccagtttg acagtcgcaa gcaaatcact ctcctagagg
    44761 gagatgcggt ggatgtctta tctacactga cagagtctta tgatttcgtc tttatggatt
    44821 ctgccaagtc taaatacatc gtctttctgc cagaaatcct caaacatttg gaagttggtg
    44881 gtgtggttgt cttggatgat atttttcaag gtggtgatgt tgccaaggat attatggaag
    44941 tccgtcgtgg tcagcgaacc atttatcgag gccttcaaaa attatttgat gcaaccttag
    45001 acaatccaga actcaccgca acattagtgc ctttaggaga tggtattctc atgcttcgta
    45061 aaaatgtagc agatgttcaa ctgtctgaaa gcgaatgatt ttcagaaaaa tttaagaaaa
    45121 aatagtaaaa tagatagagt aacacttatc tcaaaggagt agacatgaag aaaaaattat
    45181 tggcaggtgc catcacacta ttatcagtag caactttagc agcttgttcg aaagggtcag
    45241 aaggggcaga ccttatcagc atgaaagggg atgtcatcac agaacatcaa ttttatgagc
    45301 aagtgaaaaa caacccttca gcccaacaag tcttgttaaa tatgaccatc caaaaagttt
    45361 ttgaaaaaca atatggctca gagcttgatg ataaagaggt tgatgatact attgccgaag
    45421 aaaaaaaaca atatggcgaa aactaccaac gtgtcttgtc acaagcaggt atgactcttg
    45481 aaacacgtaa agctcaaatt cgtacaagta aattagttga gttggcagtt aagaaggtag
    45541 cagaagctga attgacagat gaagcctata agaaagcctt tgatgagtac actccagatg
    45601 taacggctca aatcatccgt cttaataatg aagataaggc caaagaagtt ctcgaaaaag
    45661 ccaaggcaga aggtgctgat tttgctcaat tagccaaaga taattcaact gatgaaaaaa
    45721 caaaagaaaa tggtggagaa attacctttg attctgcttc aacagaagta cctgagcaag
    45781 tcaaaaaagc cgctttcgct ttagatgtgg atggtgtttc tgatgtgatt acagcaactg
    45841 gtacacaagc ttacagtagc caatattaca ttgtaaaact cactaagaaa acagaaaaat
    45901 catctaatat tgatgactac aaagaaaaat taaaaactgt tatcttgact caaaaacaaa
    45961 atgattcaac atttgttcaa agcattatcg gaaaagaatt gcaagcagcc aatatcaagg
    46021 ttaaggacca agccttccaa aatatcttta cccaatatat cggtggtgga gattcaagct
    46081 caagcagtag tacatcaaac gaatagtcca aatcaatgag tcagggaaaa aaactcgact
    46141 tcaggaaaaa atgaagcaaa cattcccaca ataaaacgca tagtacaagg tttgtactgc
    46201 ccccaaaagt tagacaatta atttatctga aggatttagt tctgtattgc acagggctaa
    46261 gtccttttag ttttacctta attctcttat tgttctaata atcaatatag tctataatgg
    46321 cttgttccaa ttgattaagt gatttaaatg ttttctcata gccataaaac atttcggatt
    46381 ttaaaatgcc aaagaaagat tccatcctac cgttgtcttg gctgtttcct ttgcgtgaca
    46441 tagatgcttg aattccctta ctctctagga agcgatgata agaatcgtgt tggtattgcc
    46501 agccttggtc actatggaga atcgtattct cgtagtgctt ctctttgaat gcctgttcca
    46561 aca
//
LOCUS       DAVHIT010000002        45636 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVHIT010000002 DAVHIT010000000
VERSION     DAVHIT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.002"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(230..643)
                     /gene="ndk"
                     /locus_tag="VVN16_000056"
     CDS             complement(230..643)
                     /gene="ndk"
                     /locus_tag="VVN16_000056"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5571517.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENDIIQNVVHGSDSEESAKREIALWF"
     gene            complement(754..4431)
                     /gene="rpoC"
                     /locus_tag="VVN16_000057"
     CDS             complement(754..4431)
                     /gene="rpoC"
                     /locus_tag="VVN16_000057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5571518.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4464..8075)
                     /gene="rpoB"
                     /locus_tag="VVN16_000058"
     CDS             complement(4464..8075)
                     /gene="rpoB"
                     /locus_tag="VVN16_000058"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5571519.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8491..8561
                     /locus_tag="VVN16_000059"
     tRNA            8491..8561
                     /locus_tag="VVN16_000059"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8523..8525,aa:Cys,seq:gca)
     gene            complement(8759..10090)
                     /locus_tag="VVN16_000060"
     CDS             complement(8759..10090)
                     /locus_tag="VVN16_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU5571520.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVYQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELISQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(10381..11205)
                     /gene="endA"
                     /locus_tag="VVN16_000061"
     CDS             complement(10381..11205)
                     /gene="endA"
                     /locus_tag="VVN16_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU5571521.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11244..11432)
                     /locus_tag="VVN16_000062"
     CDS             complement(11244..11432)
                     /locus_tag="VVN16_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5571522.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11425..12708)
                     /gene="murA"
                     /locus_tag="VVN16_000063"
     CDS             complement(11425..12708)
                     /gene="murA"
                     /locus_tag="VVN16_000063"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5571523.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGYVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(12732..12962)
                     /locus_tag="VVN16_000064"
     CDS             complement(12732..12962)
                     /locus_tag="VVN16_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU5571524.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(13020..14057)
                     /locus_tag="VVN16_000065"
     CDS             complement(13020..14057)
                     /locus_tag="VVN16_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5571525.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(14041..14529)
                     /gene="coaD"
                     /locus_tag="VVN16_000066"
     CDS             complement(14041..14529)
                     /gene="coaD"
                     /locus_tag="VVN16_000066"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5571526.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14519..15058)
                     /gene="rsmD"
                     /locus_tag="VVN16_000067"
     CDS             complement(14519..15058)
                     /gene="rsmD"
                     /locus_tag="VVN16_000067"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU5571527.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15123..16115)
                     /locus_tag="VVN16_000068"
     CDS             complement(15123..16115)
                     /locus_tag="VVN16_000068"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU5571528.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16226..16909)
                     /locus_tag="VVN16_000069"
     CDS             complement(16226..16909)
                     /locus_tag="VVN16_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU5571529.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16965..17705)
                     /locus_tag="VVN16_000070"
     CDS             complement(16965..17705)
                     /locus_tag="VVN16_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5571530.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17739..18017
                     /locus_tag="VVN16_000071"
     CDS             17739..18017
                     /locus_tag="VVN16_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU5571531.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18095..19021
                     /gene="yidC"
                     /locus_tag="VVN16_000072"
     CDS             18095..19021
                     /gene="yidC"
                     /locus_tag="VVN16_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5571532.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19096..19890)
                     /gene="pflA"
                     /locus_tag="VVN16_000073"
     CDS             complement(19096..19890)
                     /gene="pflA"
                     /locus_tag="VVN16_000073"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU5571533.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(20016..21266)
                     /locus_tag="VVN16_000074"
     CDS             complement(20016..21266)
                     /locus_tag="VVN16_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5571534.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPEEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21333..22160)
                     /gene="purR"
                     /locus_tag="VVN16_000075"
     CDS             complement(21333..22160)
                     /gene="purR"
                     /locus_tag="VVN16_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU5571535.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22427..23368)
                     /locus_tag="VVN16_000076"
     CDS             complement(22427..23368)
                     /locus_tag="VVN16_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU5571536.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(23358..24614)
                     /locus_tag="VVN16_000077"
     CDS             complement(23358..24614)
                     /locus_tag="VVN16_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU5571537.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDNLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24616..25278)
                     /locus_tag="VVN16_000078"
     CDS             complement(24616..25278)
                     /locus_tag="VVN16_000078"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU5571538.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTAEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25241..25897)
                     /locus_tag="VVN16_000079"
     CDS             complement(25241..25897)
                     /locus_tag="VVN16_000079"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5571539.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRQEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(25908..26786)
                     /gene="rsgA"
                     /locus_tag="VVN16_000080"
     CDS             complement(25908..26786)
                     /gene="rsgA"
                     /locus_tag="VVN16_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU5571540.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(26788..27660)
                     /gene="rsmA"
                     /locus_tag="VVN16_000081"
     CDS             complement(26788..27660)
                     /gene="rsmA"
                     /locus_tag="VVN16_000081"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5571541.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(28046..28204)
                     /locus_tag="VVN16_000082"
     CDS             complement(28046..28204)
                     /locus_tag="VVN16_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571542.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEG"
     gene            complement(28197..28838)
                     /locus_tag="VVN16_000083"
     CDS             complement(28197..28838)
                     /locus_tag="VVN16_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571543.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(28840..30870)
                     /locus_tag="VVN16_000084"
     CDS             complement(28840..30870)
                     /locus_tag="VVN16_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5571544.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGLSATALLPNYREMQEMEKASDKWSQSSDRYRLSFGW
                     SSAFADEEGMRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGVEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(31138..32037)
                     /locus_tag="VVN16_000085"
     CDS             complement(31138..32037)
                     /locus_tag="VVN16_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5571545.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEELMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(32109..32669)
                     /gene="rnmV"
                     /locus_tag="VVN16_000086"
     CDS             complement(32109..32669)
                     /gene="rnmV"
                     /locus_tag="VVN16_000086"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU5571546.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(32669..33442)
                     /locus_tag="VVN16_000087"
     CDS             complement(32669..33442)
                     /locus_tag="VVN16_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5571547.1"
                     /translation="MIFDTHTHLNVEEFAGRETEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(33747..34640)
                     /locus_tag="VVN16_000088"
     CDS             complement(33747..34640)
                     /locus_tag="VVN16_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU5571548.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLETTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(34924..35058)
                     /gene="rpmH"
                     /locus_tag="VVN16_000089"
     CDS             complement(34924..35058)
                     /gene="rpmH"
                     /locus_tag="VVN16_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5571549.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(35203..36417)
                     /locus_tag="VVN16_000090"
     CDS             complement(35203..36417)
                     /locus_tag="VVN16_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5571550.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLASDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(36573..36865)
                     /locus_tag="VVN16_000091"
                     /pseudo
     CDS             complement(36573..36865)
                     /locus_tag="VVN16_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37027..37479
                     /locus_tag="VVN16_000092"
     CDS             37027..37479
                     /locus_tag="VVN16_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5571551.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(37514..38902)
                     /locus_tag="VVN16_000093"
     CDS             complement(37514..38902)
                     /locus_tag="VVN16_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5571552.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQTLDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            38968..39930
                     /locus_tag="VVN16_000094"
     CDS             38968..39930
                     /locus_tag="VVN16_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5571553.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(39959..40207)
                     /locus_tag="VVN16_000095"
     CDS             complement(39959..40207)
                     /locus_tag="VVN16_000095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571554.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDRTDEVSSKHVFEVVDRTDEVSNHTYGKAT"
     gene            complement(40271..41281)
                     /gene="ccpA"
                     /locus_tag="VVN16_000096"
     CDS             complement(40271..41281)
                     /gene="ccpA"
                     /locus_tag="VVN16_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU5571555.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(41706..42305)
                     /locus_tag="VVN16_000097"
     CDS             complement(41706..42305)
                     /locus_tag="VVN16_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5571556.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(42307..43404)
                     /locus_tag="VVN16_000098"
     CDS             complement(42307..43404)
                     /locus_tag="VVN16_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5571557.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNILIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKIDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(43404..44141)
                     /locus_tag="VVN16_000099"
     CDS             complement(43404..44141)
                     /locus_tag="VVN16_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5571558.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(44143..45027)
                     /locus_tag="VVN16_000100"
                     /pseudo
     CDS             complement(44143..45027)
                     /locus_tag="VVN16_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(45014..45208)
                     /locus_tag="VVN16_000101"
     CDS             complement(45014..45208)
                     /locus_tag="VVN16_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571559.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(45205..45411)
                     /locus_tag="VVN16_000102"
     CDS             complement(45205..45411)
                     /locus_tag="VVN16_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571560.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        13003 a        10659 c         8675 g        13299 t
ORIGIN      
        1 tattgtatcg agttaggagt ttttttggta taaccttcga cgcgcacccg catagcgggt
       61 gtttttttgt ctcgcaccta acggagcgag acaaactaat agtcacttaa tcaaaaaacg
      121 caccatatca aaaactaaaa agtttgatat catgcgtcat gtcttaaact aattgactat
      181 actttctatt caaatgagct tttaaccaat tgattgagcc aatccactct taaaaccaaa
      241 gagcaatttc tcgcttagct gactcttctg aatctgaacc atgtacaaca ttttggataa
      301 tatcattttc tccagcagct tttgcaaaat cacctcgaat agtgcctggt aaagcttctt
      361 ctggacgagt tgcacccatc atggtccgcc aagtttcgat tactttggga ccagaaatga
      421 cacccacaag aactggacct gaagtcatga attcacgaat cggtgggtaa aaactctgac
      481 caaccaagtc ctgatagtgc tggtcaatca actcttctga aacctgtgaa cgaaactcca
      541 atttttcgat tgtaaatcca cgttgttcga tgcgctttaa cacttcaccc actagccctc
      601 tttttacacc atctggtttg atgataaaga atgtttgttc catacccgtc tcctttgtca
      661 gcttctttct tttattttac cacatttcgt ggaaaaatgg agaaagtttt cagaagagag
      721 aatgagagaa ccctcgggtt ctctcattct ctcttattct actgtttctt ccacagtttc
      781 aacggcagta tccacaacta cttctgttgt ttcttcattt ccttcttcct ctactggagg
      841 attaaggtat tcttcttcgt tgacagcatg tggttcaagg ttacggtaac gggccatacc
      901 agtaccagct gggatgatct taccgatgat aacattttct ttaagtccaa ggagatggtc
      961 tttcttacca cggatagctg cgtcagtaag gacacgagtt gtttcctgga aggaagccgc
     1021 tgacaagaaa ctgtttgttt caagtgaggc tttggtaatt cccataagga ctgggcgacc
     1081 tgtcgctgga actccacctg cgataaggac atctttgttg gcatctgtaa agtcattgat
     1141 atccatgagg gtacccatga gaagatctgt atcacctgga tccatgacac ggactttacg
     1201 gatcatttga cgaaccatta cctcgatgtg tttgtcaccg atttctaccc cttggctacg
     1261 gtaaactttt tgtacttcac cgagaaggta cgtttcaact gacaagacat cacgaactgc
     1321 aaggagacgt tttggttgga tagaaccttc tgtcagagca gcaccacgcg ctacttggcc
     1381 cccaacttcg acacgcatac gagctgtaaa tggaacgaca tattcacctt cgccagtttc
     1441 acccttaaca aagactttct tggtacgagt tgatgcatct tcttcgatag cagtaacttg
     1501 tcctttaacc tctgtaataa ccgcttcccc tttaggattg cgggcttcaa agatttcttg
     1561 gacacgagga agaccctgag tgatatcggt atttgaggca accccacctg tgtggaaggt
     1621 acgcattgta agctgtgtac caggttcccc gatagattgg gcagcgattg taccaactgc
     1681 ttcaccaact tcaaccgcat caccagtcgc caagttgata ccgtaacagt gacggcagac
     1741 accgtgacga gtgttacatg taaatacaga acggatagtc acttcttcca caccagcatt
     1801 gacaatttca cgcgccttgt cttctgtaat caattcattt ggaccaataa tcactgcacc
     1861 agtttctgga tgtttaacag ttttcttagt gtaacgaccg ttgagacgct cttcgagaga
     1921 ctcgatcatc tcttttcctt ctgcgataga acggatcaag agaccacggt cagttccaca
     1981 gtcgtcctca cggatgataa cgtcttgggc aacgtcgacc aaacgacgag tcaagtaacc
     2041 tgagtcggct gtcttaaggg ccgtatcggt cataccttta cgagcaccgt gagttgagaa
     2101 gaacatttcc aataccgaca aaccttcgcg gaagtttgaa aggattggca attccatgat
     2161 acgtccattc ggagcagcca tcagaccacg cataccggca agctgtgaga agtttgagat
     2221 gttaccacgg gctccagagt ccatcatcat aacgattggg ttcttaggat cttgattagc
     2281 aatcaagcgt ttctcaagtt tttcacgggc agcacgccat tcagctgtaa cagcattgta
     2341 acgctcgtcg tctgtgatca taccacgacg gaattgtttg gtgatttgtt cgacacgttt
     2401 gtgtgattct tcaatgattt cagccttgtc atcaacgact gggatatcgg caatacccac
     2461 tgtcaatcct gcaagagttg agtggtggta accgaggttc ttcatgcggt caagtagggc
     2521 agaagtttct gtcgtacgga aacgtttgaa gatttcagcg atgatatttc cgaggttttt
     2581 cttcttgaat ggagggttga gctcaagatt gctgatagct tccttgatat ctccaccaag
     2641 tggcaagaag tatttagctg gaacaccttc tgtcaagttg gcattgtttg gttcttgcaa
     2701 gtatggtagc ccctctggca tgatatcgtt gaagagaatt ttaccaactg ttgtaagcaa
     2761 gaccttatgt ctttgctctt ctgtccaagg cttgttgagg ctgtctgttg cgataccaac
     2821 acgtgagtgg aggtgaacat aaccattgcg gtaagccata accgcttcgt cacggtcttt
     2881 gaagaccatt ccttcacctt cgcgaccagc ttcttccatg gtcaagtagt agttacccaa
     2941 aaccatgtcc tgagatggag taactaccgg tttcccatct ttcgggttca agatgtgctc
     3001 agcagctagc atgaggatac gagcttctgc ttgtgcttct tctgaaagtg gtacgtggat
     3061 ggccatttgg tccccgtcaa agtcagcatt gtaggcttca cagacaagtg ggtgcaagcg
     3121 aagagcctta ccatcaatca agactggctc gaaggcttgg atacccaaac ggtgaagggt
     3181 cggtgcgcgg ttcaaaagca ctgggtgttc tttaatcact tcttcaagga tatcccagat
     3241 acgctcatct ccgcgttcca ccaagcgttt agctgctttg acgttttgca cgatatcacg
     3301 ggcaacgatt tcacgcatga caaatggttt aaagagttca atcgccattt cacgtggcac
     3361 accacattgg tacatcttaa gagttggacc aacggcgata acggaacgtc ctgagaagtc
     3421 aacacgttta ccgagcaagt tttgacggaa gcgtccttgt ttacctttaa gcatgtggct
     3481 caatgatttc aatggacggc tacctggtcc tgtgattgga cgaccacgac gaccattgtc
     3541 aatcaaagcg tcaactgctt cttgaagcat acgcttctca ttttgaacga tgatacctgg
     3601 tgcatttaac tcaagcaaac gagccaaacg gttgttacgg ttgataacac ggcggtaaag
     3661 gtcattcaag tcagatgagg caaaacggcc accatccaac tgcaacattg gacgaagatc
     3721 tggtgggata accggaagga tgttaagaat catccattca ggtttgtttc cagacttgta
     3781 aaaggcatcc aaaacatcca aacgacggat ggctttgaca cgtttttgtc cagtagcagt
     3841 tttcaattct tctttgagtt cagcaatttc tttttcaaga tctacttgct tcaaaaggtc
     3901 ttggatggct tccgcaccca tcttagcaac aaatgaacca taaccatatt cacgcaagcg
     3961 ctctcggtat tcgcgctctg tcatgataga cttgtgctca agtggtgtat ccttaggatc
     4021 aatcaccaca taagccgcaa agtagataac ttcctcgagg gcacgagggc tcatatcaag
     4081 ggtcaagccc atacggcttg gaatcccctt gaagtaccag atgtgagata caggagcttt
     4141 caattcgata tgtcccatac gctcacgacg aactttcgta cgcgttactt caaccccaca
     4201 gcggtcacaa acaattcctc tgtaacgaat gcgtttgtac ttaccacaag cacattccca
     4261 gtcttttgta ggaccaaaga tcacttcatc aaagagtcct tcacgttctg gtttcaaggt
     4321 acggtaattg attgtttcag gttttttgac ttctccataa gaccatgaac ggactttact
     4381 tggagaagct agggtgattt gcatactttt aaaacgattt acatcaacca ctatttcttc
     4441 cctttctatt ctaagtgaac tgcttattct tgttcagcag cttcttctgt tgcttccgct
     4501 tttgttgctt tctcagcttc ttcagcttca aaggctgctt tagcctcttg ggctgctttt
     4561 tcgcgggctt tttcaaggtc atctacgtgg atgacatctt cgtccattcc ttcatccaag
     4621 tcgcgaagtt ccacttcttg gtcatcttcg tctaggacac gcatgtcaag accaagagat
     4681 tgcaattctt tgacaagaac tcggaaggat tctggaacac ctggttttgg aattggtttg
     4741 ccttttgtaa tagcttcata ggctttcaaa cgtccgttga tatcgtcaga cttgtaagtc
     4801 aagatttctt gaaggacatt tgacgcaccg taggcttcaa gagcccaaac ctccatctca
     4861 ccgaaacgtt gtccaccaaa ctgagcttta cctccgagtg gttgttgggt aacagttgaa
     4921 taaggtccga ctgaacgcgc gtgcaattta tcgtcaacca tgtggtggag tttgatcatg
     4981 tacatgactc caacagaaac acggttatca aatggttcac ctgtacgtcc atcgtaaagg
     5041 attgtcttgg catcgctatc catacctgct tctttaacag ttgaccaaag atcttcagaa
     5101 cttgctccat caaagactgg tgtcgcaatg tgaataccaa gagtacgagc tgccatacca
     5161 aggtgaagct ccataacctg accgatattc atacgtgatg gcaccccaag tgggttcaac
     5221 atgatgtcga ctggagttcc gtctggaagg taaggcatgt cttctacagg aacgatacga
     5281 gagacaaccc ctttgtttcc gtgacgtccg gccattttat ctccgacctt aatcttacgt
     5341 ttttgagcga tgtaaacacg aaccaacatg ttaacacctg attgcaactc atctccattt
     5401 acacgtgtaa agatcttaac atcacgaacg acaccatcgg caccgtgtgg tacacgaaga
     5461 gaagtatcac gcacttcacg agacttgtct ccaaagatag cgtgcaagag acgttcttca
     5521 gctgaaagat ctttctcacc cttaggtgtt actttaccta caagaatatc accttcttta
     5581 acctcagcac caatacggat aatccccatt tcgtcaaggt ctttgagggc atcttcacca
     5641 acgtttggaa tttcgcgagt gatttcttca ggcccaagct ttgtatcgcg cgtttctgat
     5701 tcgtattctt caaggtgaac agatgtgtag acatcgtcct tcaccaagcg ttcgctcatg
     5761 ataacggcat cctcgaagtt gtaaccttcc caagtcatgt aggcaacgat tgggttttgt
     5821 ccaagcgcca tttctccatt ttccatagaa ggtccgtcag cgatgaaatc gcctttttca
     5881 acgacatcac caacttttac gagagtgcgt tggttgtaag cagtacctga gtttgaacga
     5941 cggaattttt ggatgtggta aacatccaat gaaccatctt cacgacgaac ttctaccttg
     6001 tcagcatctg cgtaagtaac tttaccatca tactgagcaa tcacagccgc accagaatcg
     6061 tgggctgctt ggtattccat accagtacca acgtaaggtg cctgaggatt aatcaatggc
     6121 acagcctgac gttgcatatt ggctcccatg agggcacggt tggagtcatc gttttccaag
     6181 aaaggaatac atgctgtcgc aacggcaact acctgttttg gtgaaacgtc catgtagtca
     6241 acaatattag ctggatactc ttggttgacc ccttggtgac gtcccatgac aatcttctca
     6301 gcaaaggttc catcttcatt cagacgagag ttagcctgag ctacagtata ttcatcttct
     6361 tcatcagctg tcaaccaaac aatttcgttc gtgacaacac ctgtttcacg gtcaacctta
     6421 cggtatggtg tttgaacaaa accatatttg ttcaagtgtc cgtaagatga caagttattg
     6481 atcaaaccga tgttaggtcc ttcaggtgtc tcgattggac acatacgacc atagtgagtg
     6541 tagtgcacgt cacgtacttc atatccggca cggtcacgag tcaaaccacc aggtcctaag
     6601 gctgacaaac ggcgtttgtg agacaactca gaaagcgggt tgtgttggtc catgaactgt
     6661 gacaactgtg atgaaccaaa gaattcttta accgcagctg ttacaggacg gatattgata
     6721 atttgttgtg gtgtcaagac ttcattgtcc tgaacagaca tacgttcacg gacattacgt
     6781 tccatacgag aaagtcccaa acgtacttgg ttggcaagca attcaccaac cgcacggata
     6841 cgacgatttc caaggtggtc gatatcatct acacggccaa gtccttcagc caagttgagg
     6901 aagtagctca tctcagcaag gatatctgca ggagtcaccg tacgaacctt gtcatctggg
     6961 ttagcattac caatgatcgt tacgacgcga tctggatcag ttggagcaac aaccttgaat
     7021 ttttgaagaa caacaggctc agtcacaacg gctgcatcgt ttgggatgta gacaatcttg
     7081 ttcaagtcac catccaaatg gctttcaatg ctttcaatca cgctacgagt cataatcgta
     7141 ccagcttcta ccaagatttc tccagtttca gggtctacca atggctctgc aatggtttgg
     7201 ttgagcaaac gtgttttaac attgagtttt ttattgattt tgtaacgacc aactgctgcc
     7261 aagtcatagc gacgtgggtc aaagaagcga gccacaagca agctacgtga gctttcagcc
     7321 gtcttaggct cacctggacg aaggcgttcg taaatttctt tcaaggcttc gtctgtacga
     7381 gagtccattg gattcttgtg gatatctttt tcaacagtgt tgcgaaccaa ttcgctgtca
     7441 ccaaaaatat caaagatttc atcatcacct gagaaaccaa gagcacgaac caaggttgta
     7501 aatggaatct tacgagtacg gtcgatacga gtgtaggtga tatcttttga gtcgctttca
     7561 agttccaacc aagctccacg gttagggata acagttgaac catagcccac cttaccattt
     7621 ttgtctactt tgtcgttaaa gtaaacacct ggtgagcgga ccaactgaga aacgataata
     7681 cgttcaccac cattgatgat gaaagtaccc atttctgtca tgattgggaa atcaccaaag
     7741 aaaacttcct gggtcttgat ttcgcctgtt tctttattga tcaagcggaa ggttacaaaa
     7801 attggtgctg agtagctagc atcgtggata cgagcttctt ctagcgtgta ttttggttcc
     7861 ttgatttcat atccaacaaa ttccaactcc attgtgtctg tgaagtttga aattggcaat
     7921 acatcttcaa acacttcctt aagaccgtgg tctaggaaag ctttgaatga gtcagtttga
     7981 atttcaatca aatttggtaa gtcaagaact tctttgattc ttgaaaaact acgacgggta
     8041 cgatgtttcc cgtattgaac gtcatgtcct gccaagatga ttctcctttg taaataagtt
     8101 ccaagccttg tcaatcaggc ttttctaatc gccatatggt tgtaaacccc ttatcaccgt
     8161 gtcctcttga cgaattttca gaatctttaa gcctctgtta caaatgctca aaatcttgaa
     8221 aaaaagcaca aaaagagcag ctaaatctga ctttttcaga agatttaact gctgtgagcc
     8281 ttgtctggac aatatttcag acaaaaccta cgacaaatga ttacccatat tataccctat
     8341 ttagctagat ttttcaaggg gtttcagtag gtttttggta aattttttcc catagaaaac
     8401 ttggcatcac attcgaatca cgctatggta caaaaaactg aaaaaactat tgactgaaaa
     8461 tcattttcaa ggtataataa taaacgttaa ggcggtatag ccaagtggta aggcacggct
     8521 ctgcaaaagc ttgatcgtcg gttcaaatcc gtctaccgcc ttctataact tgatttatca
     8581 ggtttcaaat gaacagaaag cccaatttga agggcttttc ttattttccc tcgaataaat
     8641 acgtataact ttaaaaactt ttggagcgag tttgtggcag agttctttcc atggcataat
     8701 tcccttttga aatcaattga aaaaagcttc gtcacgcctg gtgacaaaag cctctagttt
     8761 attctgtttc ttcttctatt tcgacttctg tgatttggat ttttaccttg gtaacacgtc
     8821 cattcttcac tttatcattg gttaagataa actgtttgtt ttggctgatc aattcatagc
     8881 tgagtttctc agtcgttgga atggttccca ctcctgtcaa ataataacca gcgatagtat
     8941 caacgtcatc actttccagt tcaacattaa agtaattatt gaaatcattg agggtcatag
     9001 ttccttgaac aatgtaggta tcctcgccaa tctgataaac atctattgct gctttgtccg
     9061 tttcatcatc aatttcacca acaatttcct ctaagaggtc ttccaaagtc accaaaccag
     9121 ccataccacc gtattcatcc agcaagattg ccatttgtct ttgggtattt cgcagttctt
     9181 ttagcaagtc atccacaaaa atagtttcag gtacaaaaag tggatcttgt aaaattctct
     9241 tccaaacaat attgtcaaaa ccgtccacaa agcctgcctt aaggagactc ttggtgtgaa
     9301 tgattccaat tacattgtcc ttatccccat cataaaccgg gatacgagaa taattttgtt
     9361 ttaaaatact ttggataatg gcttgactat catcctgaat atccaccata aaggcatccg
     9421 ttcgaggaac cataacctct cgtgccatca gttcatcgag cgaaaagaca ccttgtagca
     9481 tctcaatctc atcagcatcc aatgtttctt cactatttgt cagcatatag gcaatttcat
     9541 cacgggtcat cttttcatcc gcatcatcga atgacatagg agtcaaatgg ctcaagaaat
     9601 tggtcgaagc agctaaaagc caaacaaaag gactgactag ttttccgatc ccaatgataa
     9661 ccggcgctgt acgaattgcc aaggcatcct ttagattaag agcgattctc ttaggatata
     9721 attccccaaa aacgatggaa atataggtca aaaatgccaa ggatagaaaa gttgccacgg
     9781 cttgtgctgt ttcgccattc ccaagccaag aggcaatcac acgtcctaga gtatcagtta
     9841 aactcgcccc tgataagatt gtaatcaggg tgattcctac ctgaatggtt gataaaaagt
     9901 ggttaggatt ttctagtacc ttcagcaggc ggatgtagcg tctgtctcct tcttccgcct
     9961 tttgttcaac tcgggcacga ttaagagaaa ccatggccat ttctgtggct gagaaaaagg
    10021 catttaatac cgtcaagata aacaataaaa caaattgtag caacaaattc tgactacttg
    10081 ggtcttccat agttcttctc ctaaaatagt atcttatcct ttattatatc acaaaattta
    10141 atccaataca tattattttt ttcttagcgt aacttccatc tctctcccaa aactggaagt
    10201 tagtctaaga cggatttcca ctgatttcac gcgtttttct catactaaaa gctaggttgt
    10261 gggaaactgg aagttagttt tagaagttgc tattttttct tagtacctgt tctagccctg
    10321 cttaaactag taaaaagaac tcctacatcc ataagtgaca taggagttta ggcttatctt
    10381 ttactgagtt acagttactt ctccagttcg gtaatccagt tgaagtccct tttgaacatt
    10441 gggaactaga acattgaatt ccaattctcc atccgaagac ttggcttcaa tctgtgaagc
    10501 tgagggaact aaatcctcgt ttgaagcgta gtaaagggtt acacggtaac ggacacgctt
    10561 gttttggtcc aaggctttac gcaccttgct ttcatagtag ttttgaccag tcgaatactc
    10621 ggcttgtgcc tgatttgccc aggctgtctg aacagcaatg tttttaggat tgcttgttga
    10681 ggcatcaaaa ccatccaaac caccgattaa ggcatagcct aacaaatgac ctctatcgac
    10741 tgcatgggta taagagccct ttagattctt gacctgatgc caacctggag gagtccaaga
    10801 agttgaacca ttcccagttt ctttacgatt cttgtactga cgagtggcat tagacaagag
    10861 ggcattagct acggttggaa cagtttcctt gcccactgtc tttgttttat tgtcagcgta
    10921 gggcttactt gaaaccttgg catctagatt tgttttatta ccattgacga taaaagcacc
    10981 tgagccattc cactccagac tcccctttat ttgactcttg actgcgtctg ttaagacact
    11041 ctctgccaat gcttgactag gagcttcagg cgcttgtttt ttctgactaa gattggtttt
    11101 gggactatta ggtgccgact gcatctgctt gatataatag ctccctgtag acaaaagcaa
    11161 taacactagc agtccgatta gtgtctgtct tgtttttttg ttcatatttc tccttatctt
    11221 tagaaaaagg ctggttctcc agcctaattt cctgtaaatt tatgaatcaa ttcctgccat
    11281 tttgctggag acaggatagc ccatggatct tgacccttgc ccaagattcc ataacctacc
    11341 attagaccga ttcctagagc cagagtacct aaaatcagta ctatgatgac taaaagtaaa
    11401 cgcttgacta cgtagcttga tttcttattc atcttcatca cttgcctcaa tccgctgaat
    11461 cttagcacct agctgagcca acttctcatg gaaaccgtag taacctctat ccaagtgaac
    11521 caatttaccg accacagttt ctccctgtgc taccaaacct gtcaaaatca aggctgcact
    11581 ggcacgaagg tcagttgaaa gaacttctgc tccctgcaaa ggctgtccac caacaatacg
    11641 agctgtatca cggataatct cagaatgcaa gcccatgcgg cgcatctctt ctaggtgttg
    11701 gaaacgattt tcgaaaactg tctccaccat ggttgattcg ccttttgcaa ctgtcatcaa
    11761 ggctgtaaat tgagcctgca tatctgttgg aaatcctggg tggggcaagg ttttcacatg
    11821 aacagctttt agattttcta gttgagaacg aacacgaatt ccttcgtctt cttcaattac
    11881 ttcaacaccc atttcaagta acttggcaat caagggacgg ttgtgctccc agacagcgtc
    11941 tcgaatcaag acataaccac cagtcatggc agcagctacc ataaaggttc ctgcttcgat
    12001 acggtcctgg actacattgt gagtcgtacc atgaagtttc tcaacaccag taatggttat
    12061 agtctctgta ccagcacctt tgaccttggc tcccatttca ttaaggagaa tggctaagtc
    12121 aacaatctca ggctcacgcg cagcattctc aatcactgtc accccatcag ccagagtcgc
    12181 tgccatcatc aagttctgcg ttgcaccaac acttggaaag tccatataga tatgagcacc
    12241 atgcaagcgt tctgcctttg cttcgatgta accagctgtc tgactaatct taacccccat
    12301 agcttccaga cctttcaaat gaagatcaat aggacggcta ccaatcgtac aaccacctgg
    12361 catggatacc ttggcatgac ccacacgggc aaggattggc cctaatacaa cgatggaggc
    12421 gcgcatctta ctgacatact tgtaaggggc ttcctcagtg atgtcgccag tagcatccac
    12481 cttgacaaga tgagcttcct catcaaagtc aaccttggca ttcaaaccac caactacctg
    12541 attcataata aagacatccg acaaaatcgg aacattctgc aagacggtct ttccttcact
    12601 tgctagaata gtcgctgcca acaagggtaa gactgcattt tttgctccct cgatcgtcac
    12661 gcttcctacc agacgattat cgccaccttg aaccacaatt ttatccatac tcgtttcctt
    12721 tactcttgat tttataataa tcctctaagc gcttcttgcc acaaatgata caggctaata
    12781 aagaaagaac tcaggatgta gcctatgaca atgctaaaaa aggctactaa taaacgaact
    12841 tttcttgtat tatcatctgt cacttttaaa accttctccc atctaacaag atcctttaaa
    12901 aggtaaaaac tcacataaat aaagagcata tgactactta gagtgaataa taattgaacc
    12961 atttgactat tataccatct tctctgtttg tgcgctagtt tggaaacttc actcaaaaat
    13021 tagggattgt ttttcaagta atcaatggca tcttgtaggg ttttaacggg cacgattttc
    13081 atatccgtct tgattgtttt agccgcttct agggctgttt ggtagttgtt tttcgcgtcc
    13141 ggatgcgcct tttgttcttc ttcgctaaca gggttatcag gagcaaagaa aatagcagca
    13201 cctgccctag ccgaagctac aaccttctta tcaatgcctc caatgtctcc cacattacca
    13261 tcgcggtcaa tggtacctgt accggcaaca atacgaccat tacgaagatc tgggtgagct
    13321 atttgagtat agatagctag actaaacatg agaccagcac ttggaccgcc aataccagct
    13381 gttgaaaagc taattgggac attgctgatt acctctgtac ggtcaatcaa gccgattcca
    13441 attccatttt tgccattttc caaggtgata atctttcctt ctgcagactt ggtttgccca
    13501 tcctcttcat aggtgacctt gacggaatcc cctaattttt gagaactgac gtaatcaatc
    13561 aagtctttgg aactatcaaa ggtctgatca ttgactgctg tgactgtatc agagatattg
    13621 agaatccctt taaaggttga attatccgtc acattcaaaa cataaactcc aaagtacttg
    13681 agttcgatat ccttaccagc tgtttttagt ccttgatact tggccatatt ttgcgatgtt
    13741 tgcatgtaga attgattgat tcgcataaat tcaacatcgg aagaaccacc tgtagtctcc
    13801 tgagcactac gaatatctgt aaaaggtgtc aaccaagcat aaatcatatg agctaaagtg
    13861 gcatgttgaa caccaaccgt aacgaattga taggcaccag cttccttatc ttctgtgtca
    13921 tttactttaa ggacttggcg aatatcttcc gaaccacctg gaacctctat ataataaggc
    13981 aagggcacta caaatgccaa gaaagtcaca atcaaggccg caatgacgta taagggccat
    14041 ctaatctttt ttttcatttc ttatttcctc caaaatactc tcgggaacat agcaggcaat
    14101 atcctgacca aacttcaaaa gctctctaac gcctgatgaa ctgatataga gatgttcagg
    14161 tcgactatgt aaataaatag tctctatatc agaagacagc tgatgattgt agtaatcaaa
    14221 actggcttca tattgcaaat ccgacgcatt cctcaacccc cgtactagga aagtagcccc
    14281 caatcttttt gcaacatcga ccaccaattc atcatgagaa gccacgactt caacattttc
    14341 cagatgtccc aaagcctttt ctagcccccg tttacgattt tcgataggaa gaaatccttg
    14401 tttgtgggga ttaaaaaaaa taccgacata gagcttatca aagagtctgc tcgcccgttc
    14461 aatgatatcc agatgcccat ttgtcatcgg atcaaatgag cctgtgaata agccaatctt
    14521 atctgacata gactgtcacc ttactaattc cataaatctt ttccttccag atacccagac
    14581 aggcaatttc ttctggaagt tcaacggcct tatccgtctc gcacacaacc ataacatctt
    14641 cagaaaaaag ctctctctca gccatttttt caatatctgc tacgatttgt tccttggcat
    14701 agggagggtc taagaaaacg aggtcaaatt ccccagatac ctgttccaat gccctttctg
    14761 catccatctt gaggagttga aattttccaa cttccttggt catctggata ttttcagcca
    14821 cgatggtctg agccttacgg tctcgctcca ccaaaacagc actggacatg ccacgcgata
    14881 ctgcttcgat agataaacca ccactacctg cataaaggtc caagactcgt cccacttcaa
    14941 agtagggacc aatcatgtta aaaatggctc ccctaacctt atccgaagta ggtcttgtcg
    15001 tcttgccttc tagtgtcttg aggggacgtc ccccatagat tcctgatacg attttcatac
    15061 tgtttattat accaaattat agacaaaaag agaaagaaaa ccgaaccttg cggttcgatt
    15121 ctctacaaaa tattttcgta agtatcgcgg acttcttgag gccaaacact tgtttgcact
    15181 tctccgatgt gtctcttgcg aagtaggaac atggccatac gagattgtcc aattcctcca
    15241 ccgattgtca atgggaatag accattcaac aaagacttgt gccattccaa ttccaagcgg
    15301 tcttcatcac ctgtaatttc cacctgacgt ctaagagttt cttcatctac acgaattccc
    15361 atagaagaca actcaaaggc tccacctaaa gactcattcc agacaagaat atcaccattt
    15421 agacccttgt agccattctc agactcgctt gtccagtcat catagtctgg tgcacgtcca
    15481 tcgtgcggtt taccatctgg caactcgcca ccgataccaa tcaaaaagac ggctccaaat
    15541 tctttacaaa tcgcattttc acgttctttc ggtgtcaagt ctgggtagcg ttctaccaat
    15601 tcttctgtat ggataaaggt gatttgtttt ggcaagatag actcgatgtc atagcgggct
    15661 tcaacagcta gctcagtcag gcgaatagcc ttataaatct tctcaactgt ttcttttaga
    15721 taaacgatgt tacgcttacc atttgggata accttctccc agtcccactg gtcaacataa
    15781 acagagtggg ttgcatccaa ggaatcctca tctggacgaa gggctttcat gtggacaaag
    15841 agaccctctc cttcaccaaa gccaaaacga gccaaggtgt ggcgtttcca tttagcaagt
    15901 gagtgcacca cttcataagt agcatcaggg atttggagaa ccttgaccga tactggattt
    15961 tccacaccag acaggttgtc ctgcattccg tcaccgacct tactcaagat aggaccttga
    16021 acttcgacaa cttctagctt atctttcaaa tactgggtaa aagtgttttt gacaaaggaa
    16081 atttcttctt gttgatggat aaaacttttc ttcatagaca actcctcaaa aatttaataa
    16141 acgtattata cacctatttc caagaaaaga aaagagaaaa tttaaaaaaa tcagtgtcac
    16201 tcaaaacact gatttcatag accttttagt cattgcgacc aaagatacgt agaatactta
    16261 ggaaaagatt gataaaatca agatagatgc tgagagccat tgacaccacc cagcctgtcg
    16321 ctacacgacc ttgtgattgt tcataggcga ggcgaatctt ttggttatcc caagcaatca
    16381 accctgaaaa aaccaaaacc atggctacgc taatcatata atcaaagaag ccactagcca
    16441 agaaaatatt gactaccata gcaatcagta aaccaatgag agctgccatc attgctcgac
    16501 caatcccact caagtctttc ttggtaaaga taccaactgc cgccatgaca aagaagagaa
    16561 gggcgctcga tacaaaggca gataaaactg tacccggagt atagaaagcc accacaaaac
    16621 taagggtaaa gccgtttaaa acggagtaaa gtaaaaatac tggaagagca gctggactat
    16681 ttcttgaagc catgctactg gcaacgaaaa ccaaagccaa ctccgcaaaa gtagcaatgg
    16741 tcaaccagag acgcccctgc atcaacaagt aaaccaactg agactgaaag accgtcaaca
    16801 taaggcctga taccaaagca gatagtccga ttcccagacc aacaaaggca taaaccttag
    16861 cgtaaaattg attgagacct gcacggtcat gtataatagt gtgattcatc cttttctcct
    16921 tttctgtgaa catctttcct tgattataac aaaaaaagtt aaaattagct taaatggaag
    16981 attaaaatac cggctgcaac tgcaacattc aaactctcgg cctgcccctt catgctaata
    17041 tggaccaact ggtctgcact ttcagccata aggggactaa ttccttgacc ctcatttccc
    17101 ataactagta caaaattttc tatagaaggc agttctctgt aatcaacaga atctttagat
    17161 agggttgttg ctagcactgg gatagctacc tttttagttt cgtcaagaag cgcttgacta
    17221 gtcatccggt aaatgggcag atggaaatga ctaccttgca tggaacgtag ggttttgaga
    17281 ctgtagatgt ctgccgactt atctgaaaca atcactcctg taaaacctgc tgcatccgca
    17341 gttcgaatga tagttcctac attaccagga tcttgcacat cttccaaaaa caagaacttg
    17401 ccctgactca aatcagcttg tcctacttct tctttttgaa ccacggcaac aattccctgt
    17461 ggagtctgag aatccgccaa atctagcaaa atatcctctg acacccagac agtttgcgaa
    17521 aaagtagcta actgatctcg ataattttct agggtaaaga ttttctcaat cgtcactcca
    17581 gcttgaacag cttcttcaaa caagtgccag ccttcaatta aataggcaga cttgcggtat
    17641 tttttttggt gtaatttctt ggcatttttt accacagaat tggcttttga ggttataata
    17701 gtcatagaaa tattataaca caatcaaagg ggtttggtat gcaaaaggtt agaatgattg
    17761 cccaaggtag ggtacaggga gtcggctttc gttggggtgt ttacagcttg gcacttgaaa
    17821 ttggtggcat cacaggtcga gtatggaata acgacgatgg cacagtggaa atcttagccc
    17881 aagcagactc atctgctatc atggcaaaat ttatccaaga aatccgaaaa ggaccgacac
    17941 ctttttcaaa agtaagctac ttagatgtca aactaagcaa ctttcctccc tactctgatt
    18001 ttaaaatcgc aaattaggtc tccagaacta ttgtatattt tgtaaaaaaa cagtagaata
    18061 gaaaggtata atttttaaag aaggaataaa aacagtgaaa tctattaaac gttttgcact
    18121 ctcggctatg ggagtggcta tgttgcttgt cttgactggc tgtgtcaatg tcgataaaac
    18181 cacaggtcag ccaacaggat ttatttggaa tacgatcgga gcgcctatgg ctgaagccat
    18241 caagtacttc gctactgata aaggtctagg ctttggtgtc gctatcatta tcgtaaccat
    18301 tatcgtacgc ttgattatct taccacttgg tatctaccaa tcatggaagg caacgcttca
    18361 ctctgaaaag atgaacgctc tcaagcatgt ccttgagcca caccaaacac gtctcaaaga
    18421 agcaactact caagaagaaa aactcgaagc ccaacaagcc ctctttgccg ctcaaaaaga
    18481 gcacggcatc agcatgtttg gtggtgtagg atgtttccct atcctccttc aaatgccttt
    18541 cttctctgct atctactttg ctgcccaaca tactgaaggg gttgctcaag caagctacct
    18601 aggcattcct ctaggttctc caagtatgat tttggtcgcc tgtgctggtg tcctttacta
    18661 tcttcaatcg ctcctttcac ttcacggagt agaagatgaa atgcaaagag aacaaatcaa
    18721 gaaaatgatt tacatgagcc cactcatgat cgtcgtcttc tccctcttct caccagctag
    18781 tgtcacactt tactgggttg tcggtggttt catgatgatt ctccaacagt ttatcgtcaa
    18841 ctatatcgtt cgtccaaaac ttcgcaaaaa agtccgtgaa gaactagcca agaacccacc
    18901 aaaagcaagt gctttctcta aaccaagtgg acgaaaagac gttacccctg aacaaccaac
    18961 tgctatcaca agcaagaaaa aacacaaaaa tcgcaacgct ggaaaacaac gttcgagata
    19021 agaagaaaaa ggcttagctg atttgctaag tctttttgca aatcaaaagc ccgatgtttc
    19081 catcaggctt cttttctatc catgtacacg tttcatataa tcttgataac tttcggtatc
    19141 catgagtttt ttagcgttct tgacgcgatc tgctgttggt ggtttgactc cttcgaggga
    19201 atatggaatt ccaagttcac gccacttgaa ctcacccatg gtgtgataag gtagaatttc
    19261 aaacttatca acatttttga gggtcttgac gaacttacca agttcaatca agtcatcatc
    19321 tctgtctgtc aatcctggaa ctagcacgtg gcgaatccag acaggttttc caatatctga
    19381 tagatactgg gcacaagcca agatattttt attggtttgg ctagtgacaa tcttgtgctg
    19441 ttcttcgttg atttccttga tatccaaaag aaccaagtca gtgacagcca tgagtttgtc
    19501 aaacttctca aggtaacgtg gtttattacg gaaaggaaga gcacaggtat ccaaggtaca
    19561 atggattcct tgttccttag ccttggtgaa gagagcaatc aggaaatcaa tctgcaagag
    19621 agcttctcct ccactgactg taatcccacc cttatttccc cagaaaccac ggtagcgcaa
    19681 ggcctctgtc aagacatcat ctaccgtccg ttcacgtgac ttattggact ccatagccca
    19741 agtgtctggg ttgtggcaat actggcaacg catgtgacag ccctgcaaaa agacaataaa
    19801 gcgaatacca ggcccatcta ctgacccaaa gctttctgtc gaatgcacca tccctgtcac
    19861 ttgtccataa tcaattgttt cttcagacat atcgttacct tccttgaaaa cgttttatag
    19921 ttttattata tcacgacttg aaggaaaaac aagatttttg cctatttttt agaaagcaat
    19981 ctgtttttca atttcatttt caatctatca tcagattatt cttcaaaatc aaagccatat
    20041 aaggttgcaa aatagtcctc ggggcgctct gcacggcgga tttgacgggc tttaccttct
    20101 tcggtataga ggatttccgc agaacgtaat ttggcattat actggtagcc cattgaaaat
    20161 ccgtgggcac ctgtatcatg aattaccagc aaatcaccga tttctgtatg aggcagttcg
    20221 cgattgactg caaatttatc attgttttca cagagtgaac caaccacatc taccgcttca
    20281 gctggtccat ctggatgggt cacgttgcta atatgatggt aagccccgta catagctgga
    20341 cgcatgaggt tgactgctga ggcatccaca cctagatagg tacggtaggt ttccttctta
    20401 tgagtcactc ttgtgactag agcaccgtga ggtgccagca taaaacgacc caattcggtg
    20461 aaaatcttga cctgaccaag acctgctgac gtaagaactt cttcatacac cttacgaact
    20521 ccctcaccaa tcaaggcgat atcgttcggc tcctggtctg gacgataatt aacaccaata
    20581 ccgccagaaa gattgataaa gtctaacgaa atgcccaact tttccttgat ttcaacagcc
    20641 agttcaaaga gctgacgagc caactctgga taatagagat gggtcacggt attggacgct
    20701 aggaaggagt gaagcccaaa agtcttggct cctttttcct tcaagatagc aaaggcttca
    20761 aagagctggt ccttggtcat gccaaactta gcctcctcag gattgtccat aatgtctgtc
    20821 cccagttcaa aaacgcctcc aggattataa cgacaagaga tgatttctgg aatgcctgct
    20881 actctctcca gatgttcaat atcttcaaag gcatccaagt taatggtcgc acccaattca
    20941 cgtgcatagg cgtattcctt gtctggcgtg ttgttggaag agaacataat ctcagaaccc
    21001 agaaagtcca gtttatggct catcaaaagc tctacataac tagagcagtc cacaccacaa
    21061 ccttcttctt ggagaatttt caaaatagct ggagttggag tagccttaac tgcaaaatat
    21121 tccttaaagc ccttgttcca cgaaaaagct tggttgacag ctcttgcctt ctcacgaatc
    21181 cccttctcat catacaagtg aaagggagtc gggaactcgg caacaatcgc ttctaactct
    21241 tctctattga taaatggtgt tttcataagc ttccttctat tctgtttgca aagaaaggct
    21301 gggacaatcg ttccaacctt tagttctatc tcttatttat cttcgtcaaa gatattgcca
    21361 acctcaacat cgatggcttg gttcttgaca tcaatattag ttaccttcaa gagtgacttg
    21421 tagtcaaact gcttttcacg ttcttcttgg gcattgtccg caaagaccgc tacacctgcc
    21481 agttctgagt cgaactcgcg caagagacta atcataccgt tgaccgttcc gccacctttc
    21541 aagaagtcat ccacaatcaa gacacggctg cctgccttaa gactacgttt tgaaaggaac
    21601 attttctcga tacggtcacc acttgaacct gaaacatagt tgacgctaac agttgaacct
    21661 tcggtaattt tcaggtcacg gcgcacaatg acaaaagaga cattgaggac attggcaact
    21721 gcatttgcaa gtggcacacc cttagttgct acggtcataa ccgcgtcaat tttttggtcc
    21781 ataaagcttt tggcaataat acgaccaata tttttcaaga tggctggtgt gctaagcaaa
    21841 tcagacagat agatataacc acctggcaag atacggtcac tttctgacaa cttggtacgc
    21901 aagtcttcaa ccatttcctt agcatcctga ctcgagatag acggtgtgaa gatgacacct
    21961 ccgccagccc cagtcactgt ctggatatga ccgatttcaa tttcctcaaa ggcgcgtttg
    22021 ataatgacga tatcttctga gatggatgat ttagcagact catacttttc agcaaaagta
    22081 ttgagactag ttagtttata aggattatta atcaaatagt tggaaatgac aaccatccga
    22141 tcacttcttc ttaatttcat ttctatttcc tattccattt ttattcaaaa aatcaaaaag
    22201 caaactagaa agctggtcgc tggtggttca aaacactgct ttgagattgt cgatagaact
    22261 gacaaaccct gtaatatacc tgcatatata catacgacaa ggcgatacta ccctagtttg
    22321 aagagatttt cgaagagtat tcatttttgt cttttactta ttataccata ttcacataaa
    22381 aaaacgaaca ttcttatcct aaaaaatgct catttttctt aaattattaa tctaaatctg
    22441 gtttatagaa ggaacgatta tccatagcga agattttatt ggacatctct cctttatcca
    22501 ccaaagccag agctgttgac atcatcatca tgcttgcatc cagattgtca atcatatgga
    22561 taatctctgc ctccataata cgtggacgga ctggacttcc atattcaagc aagccgtggt
    22621 gactgaggat gacatgacga agcaaaacga cttcttcctt ggtatcatcg atgccgagtt
    22681 ccataactgt cttggtaatt tcgctatcaa tgagagcgat atgtccaaga agattgcctc
    22741 tgaccgtgta ctcggtctgg tctggtcccg tcaactcgat gaccttagcc aagtcgtgca
    22801 acataatccc cgcatagagc agactcttat tgagctgagg ataaacttcg ctaatagcgt
    22861 ctgccaaacg taccatggtc gccgtatgat aagccaaccc cgtttcaaag gcatggtggt
    22921 tggtcttggc agctggatag gagtagaatt ccttatcata cttggtgtag agatttcgga
    22981 caatccgttg ccagacagga ttttcaattt tgaaaatcat ttgcgacatg tagtcacgaa
    23041 tttccttgac atcaactggt gacttgacct tgaaatcggc tgggtcattg ggttcaccag
    23101 cttgaggcag gcggagagta atttgattga cttgaggggt attgttataa acttctcggc
    23161 gtcctttcat gtggacaacc ttacctgcgg taaaggcctc aatgttatga ggttgggcat
    23221 cccagagctt cccatcaatc tcgccactat catcttggaa ggtaaaggct aggtagtttt
    23281 tcccagctcg agtttgcctc aggtcagctg atttgattag gtaaaagcct tcaaatagct
    23341 catctttttt catgtgacta atcttcatat tcttcctcat tttcttgaaa atggagtaga
    23401 tcaagcgcag gctcgccttc tgacaactca atgtgacgga gcgtccgctc gatagctatg
    23461 gtacgacggt ttaataattc atcaatattg ccagaggcat gttggagatg tttttgtgcc
    23521 ttgaccagaa taccaccaaa cttgccaaac tcggtcttga cactggcaag agtcttgctg
    23581 atatggtcgg cactcttttg gatattaagg gtcttgaaac caactgatag ggagttaaga
    23641 agggctgata gggtacttgg tcctgcaacg ataatctgtt cttcccgtct caaattatca
    23701 aagaagaccg gattgcggac gatttctgag tagagacctt ctgtcggaac aaacaaaact
    23761 ccaaaattgg tcgtccgagg tggtgctatg tacttgttcc taatatccct agcaaagcgc
    23821 ttgacgcttg ctaggagtga cttacgacag cgttcaatct catccttgtc acctgtctca
    23881 taggcttctt ccaagcggta ataatctgcc agtggaaact tagagtcaat tggcagatag
    23941 acgtattctt ggtcgccttg tccgggtaac ttgatggcat actccactcg ttcactagag
    24001 ttttcaaccg ttgcgtattc tcgttcgtac tgggcaggtg tcatgatgtc ttcaataatt
    24061 tgccccagtt gcaattctcc cagaatccct cgcgtcttgg ttccagagag aaccttgtta
    24121 agagctccga catcacgggc aactgtctgc atttctccaa ggccacgatt gacagactcc
    24181 agttgtttag aaactgtctc aaaggaagcc tgtaagcgtg tctgcaaggt cttttctagt
    24241 ttttcctcga ccgtctggcg catttgttcc aaacgttgct catttgattc ctgcaaggct
    24301 tggagacgtt ggtctgtctt gtctctagtt tggaggagat tatctgtcat ttcttgacgg
    24361 acttgggtca gaccttggtg caattcaatc cgcacttctt gcaaacggtc gctgacaacc
    24421 acttccaaat ctttttggtc taactggctg gcttgtctgg cttggtcaaa gcggtaatcc
    24481 aactggtctg acaaatgatc tgcctgatcc tccaagctct tacttaagtg tttctcctgc
    24541 ctatcctgcc tttgccaaat cagaaagaga ccagctaggt tggcaattaa taatagaata
    24601 agtaaacttt ccatcctacc tcctgtcctt gctatgcagt acgaccacat aaccatctgg
    24661 gcaagtcacc gacacttccc tatctatata ttcgttagaa gcgtacactt ttttaaagaa
    24721 aaaattctcc tctgtcaact catacttagc tccaagaata gtcagctggc tatcccgaac
    24781 tggcataaag gctagatagt cgtagtctga acgaggttct agctgactgg tcccttctga
    24841 acaataggca atcaaatttt gcccatcctc aatttctatt tgacgcatat agggtgccaa
    24901 cttaggattg ctaggtagaa agacattggc caacatatgg tcaatacggc cacccaaggc
    24961 accgaaaata gtgacctgag cctgaggatt ttgttcaaag atggttaaga gagccaattc
    25021 cagatctgta tcatcctttt ctggtcgggc ttggacaaaa tactgggcac gtttttgaat
    25081 cacctgtcgc tcttctgcag tcacagaatc aaaatctccg actgctagag caagaggtaa
    25141 gtcttcttcc aagacccaga gcgagcctcg atccacccca acaaaagcat caaaatctgt
    25201 ccgataatga ccgcggtttc cgcctgcaaa aactgcaacc ctagtccagt tgttttctga
    25261 gagtttgtac tcgctcattg acttctccct taaagacata ggaacctgct acaaaaacag
    25321 tcgcaccagc ttctttggct tgagcaatag tttggtcatc aatcccacca tccacttcga
    25381 tttcaaagtt taaacctttt tcctgacgaa gagcaaccaa ctcacgaacc ttatccatgg
    25441 tttctggcag aaaggcttgc ccaccaaaac ctggattaac cgtcatgact aaaacttggt
    25501 caactagatg aaggacgtgc ttgatggctt caactggtgt gccaggattg ataacgactg
    25561 aaggcttaac tccgagtgaa cgaatttttt ggagggcgcc atgaatatga ggcgttgctt
    25621 ctacatggat actgatgatg tctgcacctg cacgcgcaaa atcttccaga tgatgctcag
    25681 ggtttgacac catcaagtgg caatcgaaaa ccatcttact atgaggacga aggctctcga
    25741 ccacacctgc accaaaactg atttgcggta caaaatgact gtccatgata tcgatatggg
    25801 catattctgc cccagttgct tctagacgtt tgatttcacg ttcaaagttg gcataatctg
    25861 ctgccagaat tgacggagca atcttgtatt gagacatagg tttctcctta ttttggaatt
    25921 tttttgctga cttttttata ggtttctcta cgattttcaa tttcgctgag gaattgcagg
    25981 tagttgtcaa aacggaaggt ggcaatggtc ccctcttcta cagctggctt gacggcacaa
    26041 gacggctcat gggtatgggt acaagtacga aacttacaat ctcggctgac agtagcaatc
    26101 tctggaaagg cctgattgag gtcttcagcc cttgatactt catagtccag agatgaaaag
    26161 cctggtgtgt ctgcgatttt acccccattg agattgtaaa aactaacagc tcgagtagta
    26221 tggcgcccac gacccagact gtctgagatt tctcctgttt caagattgag atctggtgcg
    26281 attttgttga gaagagttga cttcccaaca cctgtctgcc ccataaaaac cgtaaccttg
    26341 cctgttaaca aaggcaggag ttcctcttta ctggtcacaa agtcatagcc gatgtcacca
    26401 taggtctgct ggtaaaaatc cagttctccc ctatcttcca acaaatccat tttggaaata
    26461 tagacaatgg gatggatgcc cttgtgctcc aaaagaacca agaaacgatc cagcaaattg
    26521 ctgttaaaat caggttcctt gacggacatg attactacag cttgatcgat attgacaata
    26581 ggcggacgaa ccagactgtt tttccgttcg tgaattttga ggatatagcc ttctgaattt
    26641 tcctcggcag agaaatctac ccagtcccca acataagggg tatggccttt tttacggaaa
    26701 ttcccacgcg cgcgtgtttg ataaacctgg ccatcactct ccacatagta gaaacctgcc
    26761 aaggctttaa tgatttgtcc ctgcatctta gagtccttgc cctttaagtg cgtctgctag
    26821 accggcaaat tctgccaagc tgagagcttc cccacgcaca cttggtgaca agcctgcctg
    26881 gtccaaagcc ttggtcagct tgtccttgac ctcttcagtc ttaccaaagt aacctgtcaa
    26941 gttattccac aaggtcttgc ggcgatgggt aaaactagcc ttggaaacct taaagaaaaa
    27001 gttctcatct tctactgcta cggctggctc tggacgacgc accattttca agatggctga
    27061 atccacattt ggcgctggca caaagaccgt acgaggcacg ataaaggcaa ccttggctgt
    27121 catgtaatac tgcacggcga tagacaagct accgtaagcc ttggtgttag gctgggctga
    27181 aatgcggtcc gctacttctt tctgcatcat gaccacaaac tcacaaaaag gaatgccact
    27241 ctcaatcaag tgcatgagaa taggcgtcgt gatgtagtaa ggcaaattag ccactacctt
    27301 gattggcagg tcaggatttt taaaattctg gatatgttgc gccaaatcaa ccttgagaat
    27361 atcttcgtta actacggtca cattatcaaa atcacgcaga gtatctgcca aaattggcac
    27421 caaacggtgg tcaatctcaa aagccatgac ttgggctgca cgctcagcca aaaattctgt
    27481 caaggcacca atacctggcc cgatttcgat gacattgacc tgatcatcaa tttcagccgt
    27541 atccacaatt ttttgaagga tattggtatc cgtcaagaaa ttttgcccaa aggacttttt
    27601 aaaggtaaaa ccgtgacgct ccaacactgc cttggtcacg ctataatctg caattctcat
    27661 ctattctctt ttctattatc tgttactact attgtaacac attccccttg tattttggga
    27721 atgaaaatca taccaaaaaa ctatctttcc tcatgaagaa agatagtctt ggaaatcttt
    27781 aaagagggac ttagcgcttc tctcgaaaca tacgttcata ctcttcgaaa atctcttcaa
    27841 atcacgtcag cttcaccttg ccgtactcaa gtacagcctg cggctagctt cctagtttgc
    27901 tctttgattt tcattgagta tcaaaaaaat cagcttcttg ataacttcga ttttcgggta
    27961 gggtcaaatc cacatcctcc tccacatcat ccactgtcag ttcaaccgtt tgaggtaaat
    28021 ttttcaaacg ataatgcttt tgcctttacc cttcaatact agcaaaatca agcaaatagc
    28081 catgcactcg gatttgaacc aaaaggagat gattggtcat attttccatg atgaggatat
    28141 cttctaaacc acctctggca ctaggataat cagataccct gcgaatacgg attttttcac
    28201 acatgagcaa gtttcctcat atcaatgatt tcatcagcct tattccagac taggggatta
    28261 tgggtcgcaa tgatgataat tcgattttca tctttcaaat ccaccaagag attcataacc
    28321 tcctctgaat tttcaggatc aagagctgct gttggttcat ctgccaaaat caagggtgga
    28381 tttttcaaaa tagtcttagc aagggcaact cgttgggcct ctcccccaga taaagtatag
    28441 attttttgtt ctaaatccaa ataccctaga ttaacttttt ctaaagcccc cacttgcctt
    28501 tccaaacgtt ctacttttga gattttctga ccaacaaaac ccaaatccaa attttctttg
    28561 attgattggt tttctaagag gccaaaattt tgaaagagat agcccatctg atctcgaaaa
    28621 tactcacgag tgggaatggt ttttaaatct ttcccctgat agagaaccct tcctccatct
    28681 atcttttcca gctttcccaa gatattcagc agcgtcgtct ttccgcttcc actctttccg
    28741 attaaggcat aaaccttgcc cttctccagc ttgagattca aaccagagaa aatcgcgcgg
    28801 tcattaaatt tcttttccaa tccttgaata tcaatcatct tattttcctt tcaatactgc
    28861 catgctacca gcttcttctt ttttgtcctg ccttactaaa atcaagaggg cgttaagcgt
    28921 aaacaaagct acaactagag cgctaatcaa accatctctt gtcaaaatac tagataggac
    28981 taggccaagc aagagaactc ctccttgcgc cagtaaatac ttgccatgaa gctcatagat
    29041 tgtcatacca gcaagacgtt taatcataag ctcccgtctg aactgctcaa aatagagaag
    29101 attcatggaa tcaaataaca agatagccgt agacagggtc aaaatcgtcc caataaggag
    29161 agagtaaaac tccacccgtt tgtcatttag taccttggca aaaaatagct ggctttttac
    29221 caagtaagaa accttgtgat acagaccttg ctcttttaga gctgtgatgc tgtctccata
    29281 tcgatctagt ttcaaggcac tattagccgt agttccccac aacatccctg caacaggtct
    29341 tgttccagtc gcttgcggcg ttagaactac aataatcgga tcttttaggt actggcgtgt
    29401 tgtcttgtac ccatcattat agaggaaacg ttcctgtcct gtttctgtaa aagctagcct
    29461 tacctgtggg aggtagtgtt tctgactcgt cacttctaca ctttcagatg aaaagttttg
    29521 caggtaatct gtaaacaatc cttggtagta ggcagactgc tctcgcaagc tctcaggcaa
    29581 gatcagccca aactctccct cagacaagtt ttgcatcttg tccataaact ctgaagaaac
    29641 ggtaatcttt tcctctatca gatagcgcgg tgagacatag ataacattcc ctgacggtgt
    29701 gtagtcactg agacgattgc catctaggtc cacttctact ccatctgaga aattgtcaac
    29761 attgctcata atataaaaag agtctgtatt ggctaaccgt tcttcagtaa atgtctgcca
    29821 ctcacgatta tccttacgca ttccttcttc atcggcaaat gcactagacc aaccaaagga
    29881 gagacggtaa cggtctgagg actggctcca tttatcgctt gccttctcca tctcctgcat
    29941 ttcacggtag ttggggagga gagctgtcgc actcaagccg accaccaata cagctaagag
    30001 ttgccccacc atcattaatg tcatcatacg tttgagaggg agtttccctt tcaatagatc
    30061 caccagacta ttttcctgta aaccaagtag atagacgaca cttagtaagg tagaaatccc
    30121 tgccagggtc aatccataaa gtagaagagc aatgatgacc agttgcaccg ttgccataaa
    30181 caaggcacct tgataccaga gaatccccaa tcccaaaaga ctggatacca gcactgagca
    30241 gataaactgt ctcacatctt ctaacactgg tctgagagca actccaaaca agctctcacc
    30301 agctattaag cgaatccctg cctgacgaag ggatttgatc cgataaatca gggtcagact
    30361 cataaaggtc agcagaaaaa tagctaaact cagtagcacc ataggggtgg ccgccaatag
    30421 taagactatc gaaaatggat cttctccatt cgaaacaaag ccttgatagc caagttcttt
    30481 caaggtggtc tgtaagctca ctccatccaa acttcctgac acaatcaagt aagaccctaa
    30541 taaatcactc gtttctgcac tctccttgga ggaaatggtc agaccttctg gaagctctcc
    30601 ctcaccataa gtggcatagg taaagagtgt ttctccagct tcatttggct caacgattcg
    30661 acgggcgatg agactattat gctctaccgc caagtgactc aaaacctctg tcacctcttc
    30721 gcgactagct actggttgat ttatcatcat tcctgtcaca tcaacagaag gatagttacc
    30781 aaataaaatc tcttctcgat ggtcaacgga agttatcacc atataaagcg ataccaatac
    30841 cattgaaatc aaaataaata aacgtttcat catttcctcc aaaaagttac ttggtcactt
    30901 agtgagaagg tgaccacagt cactgcgatg ttaagaagct tattaagttc tttctgctag
    30961 caacatcttc aaatacatta tatccgtttt ttcaagaact atcaaggaca tcaatgtcct
    31021 taaaatctaa ccatttcgtg tcatttttag cttatatcta aaaaacagcc ctaaagacga
    31081 actttagaac tgttttgatg cgagaacaag gacatttttg tcactgattc actatgttta
    31141 taaatatttt ttgacatcct cttgccattc ctctgtcaat ttctttacta gaaattcatc
    31201 tccaattagc tgcgcaaatg ttttagaaga ctgataaaat gattcagcta aactaaaatc
    31261 tctttcttct cttaatgctt gcttccacaa aaacataaat acaagaggtt tcttttgata
    31321 atcttgagtt ttatccataa tttcttgtaa acacgataga taatcattaa aacaatcagt
    31381 tactcctacc ttttcaagac aacacattcc tgaaataaga gtatctctaa ttataaagaa
    31441 gttttctaat ttagttacct ttttctgttc caaaagaaag agcattagct cttcgatttg
    31501 atttataaat ttggcaagat cttttctaat gagcatttga taaaaaaaca aacgaatcaa
    31561 caacatatca tttgttgaat aaacttcttt ttctatgatc tgaggcatat gttcctcaag
    31621 tattaactct gcatattcag gccttctagt tctagaagta tccaaacacg cctgcaatac
    31681 ttcacatgct aattgttctt ctttaggaag attatcaaag tatgttttat aaatctcttc
    31741 caaacacgct tccttcttat cgtactcctc ttctttacca tagattggtt cacgtaaaat
    31801 ctgatatttc aattctaaat aagcaggagg aagagctgaa aaatccggca taaggctata
    31861 aactggaact cctagccggc gagcaatata gtctaacttg ggcaaactcg gttgcgaagc
    31921 tccactttca attcgcgaca gttgacgaac tgtcagttct tgctcatctc cacaaaaatc
    31981 tggacgacta attccttttt ctatccgaat ctctttaatt ttttgaccaa ttaacatcag
    32041 acgcctcctc ttctacctgc ttcgaaaaat actgtgaaac ttaaaaaatt gtaacggcta
    32101 catctttcct actcataaga tttcatagct tcttccactt ctgccaaagt aaccccaaac
    32161 aactctaggc gtttgaggag ttgcttgccg ttagaatagc cgattcggag agtctctccg
    32221 agatattctc tacgcttacg gctgtctgcc cctgctagaa aaccaaggcg aatcaagtcg
    32281 ctacgactaa tgtcaaactg actctcatgt tcaaattgtt ctgtcacctg agctagagcc
    32341 gttttcaggt cttcatagct ggcatgctca attcccagag aacgcccctt ggtcttggac
    32401 ttgggaacag cttcatctcg cttgagaaag gcatgctgaa ctgttggaat gaccgtcatg
    32461 atcatgcgcc gaatccgttc cccattaaaa tctgggtctg taaagacaat gactccatga
    32521 cgttggtgca ggcgctgaat ccgctctata tcctgatcat tgatggcaga acctcgagtc
    32581 tcataggtct ccacatcgaa ataacgtttg agattgaccg tatcatcgcg accttcaacc
    32641 acgataactt gagaaatttt ctctttcatt acttgctgtc caatccaaaa attcgttctg
    32701 catttgcagt cgttgctacc gccagctctt ctgtcgtcat accacgcaag tcagcgataa
    32761 agtcgaccac atagcgagta taggctgttt tattttcacg accacgcttg ggtacaggtg
    32821 ctaagtaagg cgcatctgtt tccaccaaca tcttgtccaa aggtaactct ttagctgctt
    32881 cttggaggtc agttgccttc ttgaaagtca ccactcctga gaaggaaatg gtcataccaa
    32941 gatccacaaa cttctctgcc cactcaagcg tccctgaaaa tgaatgcatg ataccaccac
    33001 gaggaccaac gccctcactc ttgataatct cataggtatc ttccagcgca tcacgggtat
    33061 ggacaacaaa aggcaaatcc aagtccttag atagctgaat ctgacggcga aaaacctgct
    33121 cctgcacctc tttgggcgct gtcatccaat ggtagtctaa gccaatttca cctaaagcca
    33181 caaccttgga atgttttaac ttatccaaca agtaagcctc aacttcctct gtataagtac
    33241 cagcttctgt aggatgccaa ccaatagtcg catagagctg ctcatactca tctaccaact
    33301 ccaaggcatg ctcaatcgtc ggtttatcaa aaccaacaat attcatctgt gtcacaccca
    33361 tctcagcagc caaggcaatt tcttctgtct cacgacctgc aaattcttct acattcaagt
    33421 gtgtatgtgt atcaaaaatc atctcttcta acctcgtttt ctatcttcta ttataccaaa
    33481 aaagaggagg ggcacctaat ttttcggttt cccctcctct cttcaataga gagctattct
    33541 gctatctttt ctatccgata ttgcccatct cctattccac agttagagac agaagagatt
    33601 ggctacattg atagaatatt cctattcagc taaagtatgt gattatcttg ggttctcact
    33661 tttctcaccc atcactagga ttgatgactt ctcaaaatcc cccttataat ctaactttaa
    33721 gtagagaata gaattttcta gctgttctat tttctacgtc tagtctttct tcctagcaaa
    33781 gcaaaaccac ttgttgccac cactagacca agactagcaa gtgctgcact tgatgctgaa
    33841 cctgtgttag gtaattgttt gccagcaggt gactggtagg taacatcttg cattgttcct
    33901 gcagttgctt gcggtgcact tgttgtctta ggagcagcat taggagccac tggtctctta
    33961 ggaactgtaa ccatggcaat ggcttgcact ccaatcgttt ctgcttcctt cacatcttcc
    34021 atagttttcg cattttcaat ctctttgaga gctgcttgtt tttctgcttc cactcttgct
    34081 aaaagttctg ctttttcttt atcagaaagc ggtgcgcctt tgatttcatc ctgcttgttc
    34141 ttggctgctt tttcaatggc atttttagct gattcttttt cagtagctaa ttgcttagaa
    34201 gcttgtgatt gagctgagtt ttgtgaacct gtgtgagctg atccacccgc ctggctacca
    34261 ccgccttcat tcgcattagc atctgagctt ccagtattgc ctcccttatc tccacctggt
    34321 gtagtttcta gcttatactc tggtgtagct tcttgagtta cagattcacc tttcgcagat
    34381 tgttgaactg tatcttttcc tagaatcgtc actactgaac catctgggaa tgtgattgtt
    34441 gctgtaccat ctccagctac attgattgtc gctccgtcta atgctgaacc atttgcttgt
    34501 aaaatagcaa ccttaacttt ttcttcatct gttaaatgag ttatatcttt tacatctact
    34561 ctttgagcag gtgctttaac agttgatttt tcagcagtcg gtttagtgct atcttgtgct
    34621 tttggatctt gtgctaccaa ttgatttcct ggaattgtaa tagctgaacc atctttaaag
    34681 acgattgtcg ctgtgccatc tccagctact tcaattttat agtaaaataa aataataaca
    34741 ggacaaatca atcaggacaa tcaaatcgat ttctaacaat gttttagaaa cagaggtgta
    34801 ctattctagt ttcaatctac tgtaacatcg cttccgaatc catttgcatc tactgaactt
    34861 ttttacaaaa ataaaaatca gtagactcga gttcctactg atttttgata gaattcgttt
    34921 ggattatgca gccaaaactt tgcgtccttt acgacgacga gctgccaata cgcgacgacc
    34981 atttttagtt gacatacggt tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg
    35041 ttgataagta cgtttcacga tgaatacctc ctcatagatt ttgtattcgt ttagccggct
    35101 ataaagtgat cagttactaa acatacttta ctattctatc tgattctttc acttttgtca
    35161 atagctctag gcaaatgttt ccagcttttt cgaatgcaag ccctatctac gatactgttt
    35221 caagaaacga gtcatctttt cttggatttg ggctaactca tcaacacgag gaaggtaaac
    35281 gatacggaag tggtctggtt cctgccagtt aaagcctcga ccatgaacca agagaacctt
    35341 ttcctgcttc aagaaatcaa ggacaaactg ctcatcatca tcgatacggt acatattgcg
    35401 gtcgattttt gggaagatat agagccccgc cttgggttta acggcagaca aacctggaat
    35461 atcttgaatg gcattataga tgaaatttct ttgctcgtag attcgtccac caggaagaag
    35521 caattcatcg actgattggt gaccccccaa ggaagtttgt acgacttgtt gggccaaaac
    35581 gttagagcaa aggcgcatat tggacagcat attgagccct tcgatatagc ccttaacatg
    35641 agtcttaggg ccagacaaga ccatccaacc cacacggaaa cctgctatgc ggtgggattt
    35701 tgacagacca ttcatgctga cacagaagac atctgatgcc aagctcgcca caggcgtatg
    35761 cacatgtccg tccattacca tgcggtcata gatttcatcc gcaaagataa tcaaatcgtt
    35821 ttgacgggca atctcaataa tctccagcaa gagttcctta ggataaaggg ctccagttgg
    35881 gttatttgga ttgataagga caattgcctt ggtattggaa gtgatttttg acttgatatc
    35941 gtcaatatct gggtaccatt ccacagcttc atcacagata tagtgaacgg catttcctcc
    36001 agctaggctg acagccgccg tccatagagg atagtctggc attggcacta agacctcatc
    36061 cccattgtcc aaaagcccct gcatggacat gacaatcaac tcactgacac catttccaag
    36121 gtaaatatca tcaatatcta cgtttgggaa tttcttcagc tggcaatact gcatgatggc
    36181 cttacgggct gagaaaatcc ctttggagtt agaatagcct tcactatcac gcgcattcat
    36241 aatcaagtca tgaatgacct cgtctggcgc tgtaaagcca aattctgctg gatttcctgt
    36301 attcagacgt aaaatctttt ctccattcgc tcgcatccgc atggcttctt ccaatacagg
    36361 accacggata tcataagcaa catgctctaa cttactagac ttgttatatt ctttcatctt
    36421 gtttcctcaa ttcatcagga tagtaacatt ataccactag aaagagaatg cgacaagttt
    36481 actgaaatgt gttactagtt tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa
    36541 cgtagagata gcaggtattt tgcaaagaca catcacatac caaaagataa acctagaaaa
    36601 agcaagccta gaaaggagtc ataaagaaga ttaaacatga ggaaaagccc catatcatca
    36661 agtaatccca acgcccactt cgttttgcaa ctagaaataa gattagatgg tagagtacat
    36721 gaaagactaa aaatccaata aaacctggat aaagaagcgg taccagactc tctaattgac
    36781 agatcatgat cacttctacc aagactgaaa ctaggataat tccataatac aggaaatttg
    36841 atttttgaaa agagtttttc atcattctcc cttccttcac ttctttcctg ctatcctttc
    36901 ttttatttta tcatatattt atatagagtt taagccctaa tattcttttg aaaaatactt
    36961 tttttgcttt acttctatag tgaaaaggtt tacaattata gtaagaaaat ttcaggaggt
    37021 ttcattatgg cacaacgtta ccaaaatatc atggtcgcaa tcgatggttc taaggaagcg
    37081 gacttggctt ttgtcaaggg agttcattct gctctacgaa acgacgctaa actcaccatc
    37141 gcacatgtca ttgacacacg cgctctccaa agcgtatcca cctttgatgc tgaagtttac
    37201 gaagaactcc aagtcgacgc tgaaagtctg atgaaagagt acgaaaaacg tgctaaagat
    37261 gctggggtag cagatgttca tatcgtcatt gaaatgggaa atccaaagac cctgctagca
    37321 cgtactattc cagatgccga ggaagtggac ctcatcctcg ttggcgcaac tggtctcaac
    37381 gcctttgaac gcctcttggt cggctcttca tctgaatata tactccgcca tgctaaggtc
    37441 gatttgctgg ttgtgagaga acaagaaaaa accttataat cacaaagaaa aggagcccct
    37501 agctcctttt tgtttacgat ttatttctct ctttatggcg ttcgtaagcc ttgagctggc
    37561 gctgcagttc ctttttaata gcaggttctg gagcatattt ttcttcccaa ttatctggtt
    37621 ttaagatttt atgggtcact ggatcaaaat gagccttgcc atctggaaaa attttcccca
    37681 tattggcctg atggacaata tcaaaaatac gttctgggtc cacccccatc aagacaaaac
    37741 tgccgtaggt gaagtaaagc gtgtcaatca aggcatccac ttgccctatc aaatcttgct
    37801 gagcaggtgt cttcttggct actttatctg ctgccttatc aagggtctga tgaagttgcg
    37861 atacagcttg accaaaatct tcttcagaag gactggctgc tcgaacaaac tccaccaatt
    37921 cttctatttt aaaaccagcc ctatgggttg caccctctaa atcccaagct cgaggttctt
    37981 cttgggttcg ttcatccatc atgtggtgga aggtcttgac cttattgaaa tgatagtcac
    38041 ggctgacaaa gactttttct gaagacaaaa caccaaaatg ttccaaggcc ttgtggattc
    38101 catcttgttg attgctagtg gtaatgtgct tggcagcttc cttgacactg ctactaccat
    38161 ttcccatagc gaccgacata ccgacgccag ccagcatttc aagatcatta tctgagtcac
    38221 caaaagccat gacttggttg aggtcaaagc catattcttt cccaactcgg cgaatgcctt
    38281 ctaatttaga atttccctga ttgatgacat ccgatgcaaa aggattgcta cgtgtcaatt
    38341 tcaagtcttc aaaatcagct gccgccttct cagattcttc tggtgtcatc agcatcaaaa
    38401 cttggtagat aggctgattc atcaggtgaa gcaggtcctc ttccttttgg ggaacaacct
    38461 tgctgaccat gcgattaaaa gactgactca ccgtccgagt taaaacagag ggaacgaagc
    38521 gactaattcg ttgggaaaaa gaacccagac caaaggacat gattttagaa cccaacatgg
    38581 catccttggt ccctagagca atctccgtgc cctctttttt agcatagcta attagatggc
    38641 gcaaatgtaa cttggaaata gggctcgtga acaagactct gtctttacta aagatatact
    38701 ggccattata ggttaccgca aaatccagat ccaaatcgtc catcaattcc ttaacaaaaa
    38761 aaggtcctcg ccctgtcgct acgccaacta gtaccccttg ttctttgaca atcttaatcg
    38821 cgtccttagt ggatttcaaa acactcttgc gattgttgac caaggttcca tcgatatcaa
    38881 aaaaaacagc tttgacttcc atcctattcc aatctcccct tttgtgatac aatgattata
    38941 ccacatttca gaaagagtga gtaaatcatg cctaagaaaa tccttgtttt acatacgggt
    39001 ggaactattt ccatgcaggc cgatgcttct ggcgctgttg tgacgagttc agataatccc
    39061 atgaaccatg tgtccaaccc acttgaagga atccaagtcc acgccttgga cttttttaac
    39121 cttccaagtc cccatatcaa acccaaacat atgctggtcc tctaccagaa aattaaagag
    39181 gaagcagata actacgatgg agtggtgatc acacacggaa ccgatacttt agaggaaaca
    39241 gcctatttcc ttgataccat ggaagttccc catatgccta tcgttctaac aggagccatg
    39301 cgtagctcca atgagctcgg tagtgatggt gtttataatt acctaagtgc tttacgagtg
    39361 gccagcgatg acagggctgc tgacaaagga gttttggtcg ttatgaacga tgaaatccac
    39421 gctgccaagt atgtcaccaa aacacatacg actaatgtca acaccttcca gactccaaca
    39481 catggccccc tcggtctcat catgaaacag gaaatcctct acttcaaaac agctgaacct
    39541 cgtgttcgct ttgaccttga tcacatacaa ggtttagtcc ctatcatctc ggcttatgct
    39601 ggtatgacag atgagctgat tgatatgctg gatttagaac acttggacgg tttgattatc
    39661 caagccttcg gagctggtaa tattcccaaa gaaacggctc aaaaattaga aagccttctg
    39721 caaaaaggaa tcccagtcgc tctggtatca cgatgcttta acggtattgc cgagcctgtt
    39781 tatgcctacc aaggtggggg cgtacagttg caaaaagcag gcgttttctt tgttaaagaa
    39841 ctcaacgccc aaaaagcccg cttgaaactc ctcatcgccc tcaatgccgg actaacagga
    39901 caggctttga aagactatat ggaaggctaa tactcttcga aaatctctgc aaaccacgtc
    39961 acgtcgcctt accgtatgta tggttactga cttcgtcagt tctatctaca acctcaaaaa
    40021 catgttttga gctgacttcg tcagttctat ctacaacctc aaaaacatgt tttgagctga
    40081 cttcgtcagt tctatctgca acctcaaagc agtgctttga gctgacttcg tcagttctat
    40141 ctacaacctc aaaacagtgt gttgcgcagc ctttaaccag ccgcctagtc cgctctatgg
    40201 tattcattaa gtcaacatct cttgtttaag agcacaaaat caggaaatct tctcgattcc
    40261 ctgatttttt ctatttacgt tttcgtgttg agctacgttc tgtcaaacca tgaggtaaga
    40321 gaacttcacg ttcttccaac tcttccttat gcataatctt ggtcaacata cgcatactaa
    40381 tggcaccaag gtcataaaga ggttgggcaa tcgttgtcaa gtttggacgg gtaaagcgtg
    40441 agatttgtga atcatcacta gtaataattt caaaatcttc tggcacagaa acacccttat
    40501 cagccaaacc gttcaagact cctgctgcca actcatcacc tgtcacaact gctgcagttg
    40561 catttgatga aatcaaacgc tctgctaagg cgtaaccatc atcatagcta tatttagatt
    40621 caaataccaa accctcacta taagcgattc ctgctttttt caaggtttcc ttgtagccaa
    40681 ctaaacgaac cttaccattg atgtcatcca ctagcggacc gctaacgaaa gcaatacgct
    40741 cattttcttt agcaaggtaa ctcactgcat caattgttgc ttgcttatag tcaatattga
    40801 cacttggcaa ctggtgctca acatcgacag ttcctgcgag aacaatcgga gtacgtgaac
    40861 gcgaaaattc tgagcgaatt ttatctgtca agtgataacc catatagata atgccatcta
    40921 cctgctttga aaagagggta ttgacaacag aaacttcttt ctcgttatct tcatcgctat
    40981 tagctaggac aatattgtac ttgtacattt ctgcaatatc atcaatcccc ttagccaaac
    41041 tcgaaaaata accattggta atatttggaa tcacgacacc gacagtggtt gtctttttac
    41101 ttgcaagacc acgcgcaact gcatttggac gataatccaa gcgatcaatt acctctagca
    41161 cttttttacg ggtattctct tttacatttt tattgccatt gaccacacgg ctgaccgtcg
    41221 ccatggaaac acctgcttca cgagcgacat cataaatggt tactgtatca tctgcattca
    41281 ttccttttcc tgtcctttct atctcacaca ttcttttaca agtagaggta ctgattgaag
    41341 ctctatatct acttacaaaa gtgaagatgt gaaaatttcg ttttcatatt tctacttatt
    41401 ccattctatc actaattgta aacactttca agtgtttttt gaagattgat tgaaaaaatt
    41461 tcatagaaaa cctaggttta gctccttgct accaccttag actaaacaaa aaggaggaaa
    41521 ctaagccctc ctaaagttat agtaaaatga aataagaaca ggataaatcg atcaggacag
    41581 tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt ttcaatctac
    41641 tataggtatt gttccattca ctaccgtcaa ttttagcaca tagtcttcat gaaaatatta
    41701 tatcatcata accaaccaga ttcttttgcg atattagctg cctctgttcg attaccagca
    41761 tccaattttg aaagaatatt ggtgacatag tttcgaatag ttccgtttga gaggtaaagc
    41821 tgatctgcga tttcttggtt ggataagccc cgggcgatcc cctttaagac agcaatctct
    41881 tgttctgtca acggattggg acgggtcatc accacttcca tcaattcagg cgaatactcc
    41941 ttgcgtcctt cgaggacggt gtgcaaggtt tgcatgaggt ctgcaacgct tcgttccttt
    42001 aatacataag catccactcc agccttgacc gcacgttcaa aatacccagc acgcttgaag
    42061 gtcgtcacca caaccacctt tgtttcaagc ctttctgatc gtatccactc caagacttca
    42121 agacctgtct taacaggcat ttctacgtca aggatggcga tatctacaga ctccttttct
    42181 aatagttgga ttgcttcttg cccattcttg gcttgaagga cagactccac atctggttga
    42241 agcgtgagta attgacacat ggcatctcgc aacatacttt gatcttctgc gactaatact
    42301 ttcatcttag tttctctcct tataaggtag tcgaacttgc acttccgttg gatgtttctg
    42361 actgattaca cttacttctc ctgaaaatgg aaaaacacga tctcggattg tatggagctc
    42421 atctcctttt agaaaagcaa agccgcagcc atcatcactt actgttaaaa tgagttcctt
    42481 ctctgtccgt tctaatttta agtaagcttt agacgctttg gcatgtttga tgatattggt
    42541 caccaactca agcaaaatca tagaagccat tgactccaat tcttgagtaa ggctagcagt
    42601 atctagttgg ttatctgttt ccacctcaat tccagcaatt tctaacatct ttttcacagt
    42661 ctctagttcg gatgtcaaag ttctagactt aagattttcc acaatggttc gaacttcacg
    42721 cattgattct ttgctaattt gctgtatttc tcttaattcc ttttccactt gtggataagc
    42781 ctgcatttga aatagctgca aggctagatc tatcttgaca ctaagcatag caaaggtatg
    42841 tcccagacta tcatgcaaat cctgaccgat acgattgcgc tcattttctg ctagcaatag
    42901 atttatctgg gtattttgct tgacctgagc ttctttcaaa tcctcgacaa ggcgaattcg
    42961 aaccgagccc aaagtcatca aatcgacaaa agtaagaatt acaagcatat agactagaaa
    43021 ctcaacttcg attctctgaa aaatcaaaag tcgccccaca acaaggactt gagcaaggag
    43081 aaatgtccat acatgtaaag actttaaact acgtacgcca aaatgataaa ttaagatatt
    43141 ggaaaggaaa aagaaaaacc agatataatt cccagcaaca aaggcggtat tcccagctac
    43201 atagaccagc atgatccccc agaaaagcca agataggcgc tggttcttag ttattaaaac
    43261 acccaaatac gtcactacaa atagaatatc aaccaataaa tgccaagcag gaagctcccc
    43321 aatcactaca ggtaggatgg gaaaaagcat aaaaatcaaa ctggtccaaa acatatagtg
    43381 tatgcttttc agtctttcaa gcattaagca ttctccttat gaccttgaag gtaaatagtc
    43441 aaaccaaata aaactgctga aaaaacaagt aaataaactg tggctgatag attgatgcca
    43501 ccctcgttta agaaggtctt gagcaactcc atcaattgat aggtcgggag acacttacca
    43561 actgcttgca tccagtctgg aaataaagaa atgggcatcc agagtccgcc taaaacagcc
    43621 aatcctagat aaagaagatt gcccacaaca gacatcagct gactagttgg taagagagtc
    43681 aaggtcaagc caagtgctac aaaagcaata ctccccacaa tcagcaaaat cgcagctcca
    43741 acccatttta ctagaggcat gtctacacct cttacaaaat gtccaactgt aaaaacaacc
    43801 agaattgaga ccaaataatc aaccagcata cttgttatct ttgatagata atattctacc
    43861 atatttacag ggctatgacg caatgttttc tgccagttgt tcatcttatc ggtatgtaaa
    43921 acagctggaa atgagaagat agttgtcgac atcatggaaa aggcagtcat ggagatgagg
    43981 taatcacgca taaaattcgc gggttcacct ggtgtgtcct gatagatacc agaaaaaaat
    44041 aaatagaaag ccgtcggcat ccctaccgac aatagataat agattaattg tcgtttggtc
    44101 aataaaaatt ctatcttact aagtgctatc catcgtttca tcttagttat ctcccttctg
    44161 cgtctcttca aagattgtat tcaacaaact acgattatta acttcgattt cttgcatcct
    44221 acatcctgct tgggctaata gttcccagaa agcatctgct tctcgagtga ctacttataa
    44281 agaatcttgc tttagggtcc agttttcaac caaatttgac tgctctacga cttccttgta
    44341 agctatagga aggataaagt gcttttctat ttcctcgcta cgcatagcta gaggcgtcgt
    44401 atctcgaatc aactctccct tatttaagag caaaatgcgg tctgcagtgt gttccacttc
    44461 ctcaatataa tgggacgaat agagaatggt gactccttgc gcttttaact cctggacaat
    44521 ttcccaaaaa cgttgacgag ttgaggtatc catggacgca gtaggctcat ctaaaaaaac
    44581 aatctttggt cgcccaatta aggtcaagac gaaagaaaag agacgctttt gaccgcctga
    44641 taatttttct gccaactgtt ctttttgttg cttgacaaac tgcaatagtt gatcaatttc
    44701 ctgattgctc aaaggatttg gatagatacg ttgaaagaaa gcaatcaact ctttaacttt
    44761 taatttctga acgattacat tttcttgagg gaggtaagcc ctagtatagt ctaacttaga
    44821 actcgtcact ggtaaacctt gaatagatac ttgaccgctt gtgaccagtt tatctccaag
    44881 caaacaatct aagagtgttg tctttccggc accatttgga ccaattaagg caacacattc
    44941 accatcgttg atttcaaagg agatgttccg caaaatttcc ttgtctttta ttttcttact
    45001 caatttctca actttaacca cagtcatgag actctccttt caaccactcc attcccatag
    45061 ggaaaacgac gaaaatcata aatccaaacc ccaaagcacc acaaatgaat tggcgaagca
    45121 aggcttgatc aaaccaatca gtaaacattt ccactaacca taccaagagt gacaggccga
    45181 taaagaaata gatgatccct ctcttcattc ttcaaactcc tttttcacat ctctgactaa
    45241 tttcaaacct tctctaacaa gccaagacat gattccaaag ccagcaaaga ccttccaagg
    45301 aaaatggtag aaatcctcat ccaatcccga aaacatgaga taggtcataa ctcctgctac
    45361 tactaaactc actgcgataa tcattttatt tctcatctct tcttccccca tttcatacta
    45421 caattatagt cttttaaaat cagaggaggc agaagcttct gtcactagaa aatatgacaa
    45481 atgtcataaa aaattttgtt caaaacaagc aagatacact atacaataaa acacaatcag
    45541 aaaaatctaa ggcaacttcc tcaaaagaga tatcaaaccc aattcacacc ataatgtaaa
    45601 ctaatactta tttaaaatca aaaagagtag aaattt
//
LOCUS       DAVHIT010000003        45484 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVHIT010000003 DAVHIT010000000
VERSION     DAVHIT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.003"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(40..354)
                     /locus_tag="VVN16_000103"
     CDS             complement(40..354)
                     /locus_tag="VVN16_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU5571561.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(484..723)
                     /locus_tag="VVN16_000104"
     CDS             complement(484..723)
                     /locus_tag="VVN16_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU5571562.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAQGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(775..2184)
                     /locus_tag="VVN16_000105"
     CDS             complement(775..2184)
                     /locus_tag="VVN16_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU5571563.1"
                     /translation="MGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVAREITSGVLE
                     GKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEPNEEGLQFYD
                     NLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVVFKRYKDKVK
                     YWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVASARAVKIGHE
                     INPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYPEHIFKYWER
                     KGISVDFAAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETEDLVKNNYVK
                     ASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVTADGMVHDDYRIEYLDA
                     HIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDNGKGSYNRSP
                     KKSFGWYKEVISSNGESVE"
     gene            complement(2400..2738)
                     /locus_tag="VVN16_000106"
     CDS             complement(2400..2738)
                     /locus_tag="VVN16_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5571564.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            complement(2894..3046)
                     /locus_tag="VVN16_000107"
                     /pseudo
     CDS             complement(2894..3046)
                     /locus_tag="VVN16_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3394..3786)
                     /gene="rpsI"
                     /locus_tag="VVN16_000108"
     CDS             complement(3394..3786)
                     /gene="rpsI"
                     /locus_tag="VVN16_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5571565.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(3805..4251)
                     /gene="rplM"
                     /locus_tag="VVN16_000109"
     CDS             complement(3805..4251)
                     /gene="rplM"
                     /locus_tag="VVN16_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5571566.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            4606..4923
                     /locus_tag="VVN16_000110"
     CDS             4606..4923
                     /locus_tag="VVN16_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU5571567.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(4968..5780)
                     /gene="folK"
                     /locus_tag="VVN16_000111"
     CDS             complement(4968..5780)
                     /gene="folK"
                     /locus_tag="VVN16_000111"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU5571568.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(5823..6377)
                     /gene="folE"
                     /locus_tag="VVN16_000112"
     CDS             complement(5823..6377)
                     /gene="folE"
                     /locus_tag="VVN16_000112"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5571569.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(6358..7680)
                     /locus_tag="VVN16_000113"
     CDS             complement(6358..7680)
                     /locus_tag="VVN16_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU5571570.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMADY
                     RSLLEGGAAASLQGTTEFEIITAIAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLGAIAEQKAGIIKQGIPLVTGRIAPEALVVIDRIAEGKDASR
                     LAYGADYQVSHQKSVVTGEIFDYTSAVRQGRFQTGLLGLHQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLDQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(7682..8662)
                     /gene="folP"
                     /locus_tag="VVN16_000114"
     CDS             complement(7682..8662)
                     /gene="folP"
                     /locus_tag="VVN16_000114"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5571571.1"
                     /translation="MVKSLEGVTDMSTKVNHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGVGQAFTEEELADFEKMPIEDLMETFFERALARANQAGIAQENILLDLGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFDVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(8756..9463)
                     /locus_tag="VVN16_000115"
     CDS             complement(8756..9463)
                     /locus_tag="VVN16_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5571572.1"
                     /translation="MEEKNMWKELLNRAGWILVFLLAAIVYQIPLAVTSILNLNEVPL
                     LQSGLIVAGISIVVLALFIIGARKTQLASFNFSFFRAKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTGNQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTVVFALLHQPSNLPSLLIYGGMSIVLSWTAYKTQRLEMSILLHMIVNGVAF
                     CLLALVVIMSRTLGISV"
     gene            complement(9521..10939)
                     /locus_tag="VVN16_000116"
     CDS             complement(9521..10939)
                     /locus_tag="VVN16_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU5571573.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGVISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYIVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTVFGAALGSEGLGALISDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSVKLDKALYSDLIGTSIGA
                     IAGTSNVTTYIESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLASLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQAK
                     DVHVMIWILDALFILNYISMAL"
     gene            11129..11941
                     /locus_tag="VVN16_000117"
     CDS             11129..11941
                     /locus_tag="VVN16_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5571574.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKITEKGHQVIITTG
                     RPYRMSKDFYRELSLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVRRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFKPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKVMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLSYADEQIALTNDQDGVAKTLQDLFL"
     gene            11952..12032
                     /locus_tag="VVN16_000118"
                     /pseudo
     CDS             11952..12032
                     /locus_tag="VVN16_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            12364..13383
                     /gene="adhP"
                     /locus_tag="VVN16_000119"
     CDS             12364..13383
                     /gene="adhP"
                     /locus_tag="VVN16_000119"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU5571575.1"
                     /translation="MKAVVVNPESTGVAVEEKVLRPLETGEALVEIEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGVGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADVVINGLEVD
                     DVPGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            13617..14606
                     /gene="manX"
                     /locus_tag="VVN16_000120"
     CDS             13617..14606
                     /gene="manX"
                     /locus_tag="VVN16_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU5571576.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            14634..15437
                     /locus_tag="VVN16_000121"
     CDS             14634..15437
                     /locus_tag="VVN16_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU5571577.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            15461..16372
                     /locus_tag="VVN16_000122"
     CDS             15461..16372
                     /locus_tag="VVN16_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU5571578.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(16534..17868)
                     /gene="pepC"
                     /locus_tag="VVN16_000123"
     CDS             complement(16534..17868)
                     /gene="pepC"
                     /locus_tag="VVN16_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU5571579.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(18085..18807)
                     /locus_tag="VVN16_000124"
     CDS             complement(18085..18807)
                     /locus_tag="VVN16_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU5571580.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(19075..19305)
                     /locus_tag="VVN16_000125"
     CDS             complement(19075..19305)
                     /locus_tag="VVN16_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5571581.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(19315..20556)
                     /locus_tag="VVN16_000126"
     CDS             complement(19315..20556)
                     /locus_tag="VVN16_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU5571582.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(20578..20730)
                     /locus_tag="VVN16_000127"
     CDS             complement(20578..20730)
                     /locus_tag="VVN16_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571583.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGLF
                     SALKEK"
     gene            complement(20751..21029)
                     /locus_tag="VVN16_000128"
     CDS             complement(20751..21029)
                     /locus_tag="VVN16_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU5571584.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPTRY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(21022..21285)
                     /locus_tag="VVN16_000129"
                     /pseudo
     CDS             complement(21022..21285)
                     /locus_tag="VVN16_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(21387..25778)
                     /locus_tag="VVN16_000130"
     CDS             complement(21387..25778)
                     /locus_tag="VVN16_000130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU5571585.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLDRLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(25882..27963)
                     /gene="fusA"
                     /locus_tag="VVN16_000131"
     CDS             complement(25882..27963)
                     /gene="fusA"
                     /locus_tag="VVN16_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5571586.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(28388..28858)
                     /gene="rpsG"
                     /locus_tag="VVN16_000132"
     CDS             complement(28388..28858)
                     /gene="rpsG"
                     /locus_tag="VVN16_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5571587.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(28878..29291)
                     /gene="rpsL"
                     /locus_tag="VVN16_000133"
     CDS             complement(28878..29291)
                     /gene="rpsL"
                     /locus_tag="VVN16_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5571588.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            29708..33505
                     /gene="spuA"
                     /locus_tag="VVN16_000134"
     CDS             29708..33505
                     /gene="spuA"
                     /locus_tag="VVN16_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU5571589.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(33605..34654)
                     /locus_tag="VVN16_000135"
     CDS             complement(33605..34654)
                     /locus_tag="VVN16_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5571590.1"
                     /translation="MVELGISTFGEITELERTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(34773..36581)
                     /gene="glmS"
                     /locus_tag="VVN16_000136"
     CDS             complement(34773..36581)
                     /gene="glmS"
                     /locus_tag="VVN16_000136"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5571591.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVAPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYSMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(36773..38152)
                     /gene="bglA"
                     /locus_tag="VVN16_000137"
     CDS             complement(36773..38152)
                     /gene="bglA"
                     /locus_tag="VVN16_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU5571592.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGICLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            complement(38252..40105)
                     /locus_tag="VVN16_000138"
     CDS             complement(38252..40105)
                     /locus_tag="VVN16_000138"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5571593.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(40118..41377)
                     /gene="rseP"
                     /locus_tag="VVN16_000139"
     CDS             complement(40118..41377)
                     /gene="rseP"
                     /locus_tag="VVN16_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU5571594.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(41399..42202)
                     /locus_tag="VVN16_000140"
     CDS             complement(41399..42202)
                     /locus_tag="VVN16_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5571595.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(42211..42969)
                     /locus_tag="VVN16_000141"
     CDS             complement(42211..42969)
                     /locus_tag="VVN16_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU5571596.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(43200..43664)
                     /locus_tag="VVN16_000142"
     CDS             complement(43200..43664)
                     /locus_tag="VVN16_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5571597.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(43753..44724)
                     /gene="ruvB"
                     /locus_tag="VVN16_000143"
     CDS             complement(43753..44724)
                     /gene="ruvB"
                     /locus_tag="VVN16_000143"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5571598.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            44885..44977
                     /locus_tag="VVN16_000144"
     CDS             44885..44977
                     /locus_tag="VVN16_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU5571599.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT        13394 a        10422 c         8552 g        13116 t
ORIGIN      
        1 tatcttaact taaatattta ttgacatttc atgtgttcat caaatattct caagaatcaa
       61 attagccatt ttttcaattc ccattggaat aggaatatag gcttgaggag gtatttgtac
      121 aactggtttt cctgctttag aaccagcctc ttcaaattgc ttaaagtaca tttttgtttg
      181 aggactgaca agatacaaat caaaagctgc tgctgcgata gctttccctc cttcagtagc
      241 actaatagca tcaactacaa tatctttccc ttttcctttt agaaactctg ttgttttctg
      301 tgccataagt gatgaagaca ttcctgctgc acaaataatt aaagcttttg ccataatatt
      361 ttctcctttt cttaaatcca atcaaagctg tgctaagttg gcttatttgt tatctatttt
      421 tattataaaa taaagcgttt ccaatgacaa ttccctcatt ttcctaaatg atatggaaaa
      481 aaattattta tacttcaatt tataaaataa aattattcct gagagtagaa atgaaacact
      541 atttgctaaa atcaaaggca agtctcctat acgaatacct tgagcaagcc acaatgcaat
      601 accaataact tgcataacat acatacctag agcaatagat cctgtgtcct ttgtcttaac
      661 tacacgaaaa acttgtggta aaaatgcaaa tgttgttaaa attgctgcaa tacttccaat
      721 catatgtcac ctcaatatgc taaacaaact gagaataatc tcagtttgtt tatactattc
      781 tactgattca ccgttagatg aaataacttc cttataccag ccaaaagatt ttttcgggga
      841 acgattataa cttcccttcc cattatcatc tttatctaca taaataaagc cataacgttt
      901 ccgcatttca ccggtaccag ctgaaaccaa atcaatacat ccccatggag tataacccat
      961 taaatcaaca ccatcttcaa ctacagcctt tttcatttca cgaatatggg catctagata
     1021 ttcaattcta taatcatcat gtaccatacc atctgctgta acttgatcta tagctccaaa
     1081 accattttca acaataaaga gtggtaagtg atagtggtct gtaaaccaat ttaacgcata
     1141 acgcaaacct tctggatcaa tttgccactc ccattcagaa gccttaacat aattattttt
     1201 cactaaatct tctgtttcaa gataatcaaa ataaggatta ttttcacgat gagagtcgat
     1261 agcaaaggac atatagtaac tgaaaccaat gtaatctaca gtcccaccaa gtaaatcttc
     1321 tttatcctgg gcagcaaaat caactgaaat accttttcgt tcccaatact tgaaaatatg
     1381 ctcaggatat ttacctaaaa catgcacatc agcaaaataa taacgcttct gcatagcttt
     1441 cattgccatt aagatatcct taggattgca agtagctgga taaattggac acatcgcaat
     1501 catacaacct atttgaaaat ctggattaat ctcatgacca atttttacag ctcgtgcaga
     1561 agcaactaat tcgtaatgtg ctgcttgata cataattgct tctctattat caccttcctc
     1621 atatacaata cctgagttag taaatggtgc aaaatcttcc tgataattcg cttgattatt
     1681 gatttcattg aaagtcatcc aatatttaac cttatctttg taacgtttaa atacgacttc
     1741 tgcaaaacga gcaaagaaat caatcaattt cctatttttc caaccaccat attcggtcac
     1801 taagtgataa ggcatttcaa aatgagatag agtgatgaca ggttcaatac cattctttaa
     1861 gcattcatca aaaagattat cataaaactg taatccttct tcattcggct ctaactcatc
     1921 accttttgga aagatacgcg tccatgcaat agaggtacgg aagcacttga atcccatttc
     1981 agcaaaaagt gctatgtctt ctttataacg atgataaaaa tctatcgcct catgatttgg
     2041 ataatattta ccctctaaaa ctcccgaagt aatttcacga gctactccat gacgaccagc
     2101 agtcataaca tcagcaacac taattccctt gccaccttct tgccatccac cttcaagttg
     2161 atgagcagca acagcaccac ccataaaaat ccatctttaa aagtagtcat cttttttcct
     2221 cctgactttg atactcttat tataaacctc aaaccaaaag atgaaaacgc attctttttc
     2281 cttattgtta aggaaagaag taatttttaa tggaaataga acaatatctt cttgtattct
     2341 cgtaatgata tctttacgat tttcaatact ttcaaactac aaaaactctc acaataattc
     2401 taattccctg tgtctataaa cgacttatcg ctttctggca tcccagaatc atcttctata
     2461 taacgttcaa cttgcatctg caagtgatat ttttttctta aatctaagat tttctgcatt
     2521 gtctttgatt gatgatgttc atctaaagct tcttgattta tccactgatc aataaggaga
     2581 atagttccct ctttttcaat tggtaaaaaa tattcgtatt tcaagttacc tttttgattt
     2641 ctaatttctt caacaaggcc actatcaagc atttctcttg caaactttat tgcactatct
     2701 ccatcacctt tataatatac atgaatagtc aatgtcatct tatatccttc aaaatcatcc
     2761 ttcaatttta aaaaaacaag tttagatgag gatctaaact tgttttttat gaactaatta
     2821 tctaacgttt cgccattact ttcaatcact tctttatacc aataaaatga ttttttctta
     2881 tagcgattta tagtcaattg aaacaagagc aggacaaaag agcccatgtt ttctcaatag
     2941 gattgtactc aggtgagtag ggaggaagag gtaaaagttt atgcccaaac tcttcacaca
     3001 agagttctaa cttacccatt ctatggaatc ttgcattatc cataatatag tagattgaaa
     3061 ctagaatagt acacctctac ttctaaaaca ttgttagaat tcgatttgac tatcctcagt
     3121 ttgtcctatt cttatttcac cttactataa aaataaggta aacttttcaa aaaaacacga
     3181 aaaaagcacc cataaggtaa acttttgagt gctttgaaac gttgatataa tcgtattgat
     3241 taacgttttg aggcaactga ccttgtcagg ttgcagccac atttgtaagc gactaaagtc
     3301 gcaacaactg tgtcaattgc accaatttag taagaaagta taaaaaagaa caccccgaaa
     3361 ggtgctctgt ataagtatag taattctttc gaattaacgt ttactaaatt gtgatgcttt
     3421 acgagctttc ttaagacctg gtttcttacg ttcaacttta cgtgagtcac gtgtaagaag
     3481 tcctgcgcgt ttcaatgaat cgcggaagtc tgggtctact tgaagaaggg cacgagcgat
     3541 accatgacgg atagctcctg attgaccagc gtatccacca cctacaacgt taacgaaaac
     3601 gtcgtatgaa cctacagttg aagtaactgc gaatggttgg ttgatgacaa gacgaaggtc
     3661 agcgtgtggg atgtactctt caacatcttt tttgttaaca gtgattttac cagttcctgg
     3721 aacaaggcga acgcgtgcaa cagcgttttt acgacgtcca gtacctgcat attgtgcttg
     3781 tgacatactt tattgttcct ttccttagat aagtcctgaa atgtcaagaa cttctggttg
     3841 ttgtgcagcg tgagtgtgct cagctccaac aaatactttc aacttcatac cttgagcgcg
     3901 tccaagtgta ttgtgtggaa gcataccttt aactgatttc tcaatcaaac gtactgcatt
     3961 tttagaacga agttcacctg cagagatttg tttcaatcca cctgggtggt ttgagtgagt
     4021 gtagtagatt ttatcagttg cttttttacc agtcaattta actttttcag cattgataac
     4081 aatcacgaag tcacctgtat cagtgtgtgg tgtaaatgtt ggtttgtttt ttccgcgaag
     4141 tacgctagca actactgcag aaagacgtcc aagtggtaca tcagttgcgt caactacgta
     4201 ccatttacgt tcaacttggc ctggtttagc cataaatgtt gttttgttca tgatttctcc
     4261 tatatatagt tcgatttttg tttacggtga tgggtgttcc ttcccatcga aaagtatttg
     4321 gaaggttccg gggcctttca aatggggtaa acaataccgc ctactatcat accaaatttc
     4381 acccctaaaa gtcaatagat atgaaaaata tttttcggta acttctcaaa ataactgaaa
     4441 gttatataat caagttaatt tgggaagtta cctattaact tcggtagatt tttgggaatt
     4501 tttccctatt tctccctgta actaaaatcc ttttcaaaaa atcacctttt cttgattttt
     4561 aatcctattt gctgtatgat aaggaaaaag aaaggagaca gagatatggc ttttaccaat
     4621 acccacatgc ggtctgctag ttttggcatt gttaccagct tgcctgatga catcattgac
     4681 tctttttggt atattatcga ccatttctta aaaaatgtct ttgaattgga agaagaactc
     4741 gagtttcaat tgcttaataa ccaaggaaag attaccttcc acttttcaag tcaacacctc
     4801 cctacagcca ttgattttga ctttaaccat cctttcgacc ctcgttatcc cccaagagta
     4861 ctggttttag acatggacgg tagagaaact atcctcctcc cagaagaaaa tgacctattt
     4921 taaaaactct agccttcagt tgcaagtaac tgaaaactag agtttttcta ttttttcaaa
     4981 gcatcataca agttgcggat cggttgtttt aatatcggat ggataaaatg aggcgcaatt
     5041 tcctgtaagg actcaaggac aaaaaggcgt tccgctatgt aaggatgagg caatatgagg
     5101 tcgtctgtat aaaggatctg gtcctccaca aagagcaagt ccaaatcaat caaacgaggt
     5161 ccccaatgca cttctctcac ccgtcccagc tctgactcaa tggctaacaa ggtttctaac
     5221 aagtcttgtg ctggtagcca ggtttccacc tcaaccactt gattggcaaa gctatcctgc
     5281 tccactccac cccaaggctc cgtcgctaag acactggact ctttgagaat atggatgcca
     5341 cgagctcgca gtttgtcaat ggcttgcttc aagtttgctt gcttatctcc catattgctt
     5401 cctagggcga taaaggctcg ttgcttgcgg cgatgaatgg ttaccgagca agtatctagt
     5461 gacaaatgca ccggtgccca aggttttttc agttccaact tcatttcttg gacaagagga
     5521 taagactcaa aggtacgttc caccagttta taggctaccg tttcaatcaa atcctcactc
     5581 gtttcctgaa accaagtcgt ccactgctga cacaattctc cgtaatggac agaggctgtt
     5641 aaatccaagt ctgtagctgc cttggtcata tcataggata ggatggctga aacgacaaat
     5701 ttctgcccca attctttctc actaggaaaa agaccatgat aggcaaacat ttccaaatcc
     5761 ttaatctgca gttgatccat aatgattcct ttctagaaaa atccgcttga agcggattct
     5821 ttttatagcc ccattaaacg ataagcttgg tcgcggaggt ccttatctgt ttcaaataga
     5881 ccacgagcta ctgtcgtcaa ggttgcagtg cctggttttc taacaccacg catactcata
     5941 cacatatgtt ccgcctcaat gacaacaaag gctcctttag cacctagata gtccatcaag
     6001 gcatcggcca cttcgatatt caaacgttct tgaatttgtg gttttttcga ataaacttca
     6061 accgtacggg ctagcttaga caagcctgcc acacgaccat ctggaatgta ggcaatgtgc
     6121 gctctaccat aaaatggcaa gaagtggtgt tcacacatgg tatggaaaaa gatatccttt
     6181 tctaccacca tattatcgtc aataatttca aaggattttg acaaatgttc ctctgctgtt
     6241 tgaccaagac ctgaaaaaat ctcttgatac atacgggcta cacgagcagg tgtttcctgc
     6301 aagccctcgc gattagcgtt ctctcctaca gcctcgataa tcatttttac agctgcttca
     6361 atcttttgtg tatccattct cgttcttcct ctccatcaga taggccctca cttggctcaa
     6421 gaaatacaag gaacctgtga caatcctaac tgtttgtttc tcttcttttt tatctgtcaa
     6481 attctgatct agaaaatcat gccaatcttg gtagctgaga tttctagact tagctgcctc
     6541 tttcagcacg ctttcatccg tcgcccgact atccgcaaaa tgtgttagag taagctcggt
     6601 atctggcatg gctcccaaca agtccaacat atcctccaag gccttggttt tgatacaagt
     6661 gaagaggatt tccttatgat aatctgcaaa acgttcttgc aaggttacca acaaggcctt
     6721 gatagcatgg ggattgtggg ctccatccaa aatcatcaag ggatctcttg acacgatttc
     6781 caaacgcccc ggccaacttg tttcttccaa ggcttgacca agaaagtcat tgcttgctag
     6841 ctctcgacca tcttcttgac aaaaagtatc aagtaaagct atggccatcc cagcattctc
     6901 tatctggtgc aaaccaagca ggccagtctg gaagcgacct tgtctgacag cacttgtata
     6961 gtcaaagatt tcacctgtaa ccacactctt ttgatggctg acctgataat ctgccccgta
     7021 ggcaagtctc gacgcgtctt tcccttccgc aatgcggtca atcacaacca aagcttctgg
     7081 agcgatacga cctgttacca agggaatacc ttgtttgata ataccagctt tctgctctgc
     7141 tatggctccc aaggtgtcac ctaaaagggc tacatggtcc aatccaatag tcgtaattcc
     7201 tgtcaaaatg ggttggcaga cattggtact atctaaaaga ccacccatgc cgacttccat
     7261 gatagccaca tctacttgct ctgaagcaaa gtagtcatag gctatggctg tgataatctc
     7321 aaactcggtt gtgccctgta aactagcggc tgctcccccc tccagcaaag accggtaatc
     7381 cgccatgagg gcttctagtc tagcctctgg gatagattcc ccattgatac taatctggtc
     7441 tgtataatga atgagatagg gcgagctgaa aacgccaact ctcagcccta gtttttccag
     7501 catatttttc aaaaaagcaa tggtagaccc cttgccattg gtccctccaa tatggatgac
     7561 cttgagtttg agatggggat tgccacgcaa agctaacagt tccaccattc gttccaagcc
     7621 aaaatgcggt tggtccgtcc ggtagttagc aatccactga ttgttttcaa tttctttcat
     7681 cttatttata ttgttttaaa tctaaatttt ccgcttcatc agccagacga atagcggagg
     7741 caatttcaac tgccatcctg tgacttgcta cgtcatgcac gcgcaccact tctacaccct
     7801 gtcttgcagc aatactagtt acatgagccg aagctgtatc tcgattgcgg aaaccaagtt
     7861 ctgtctcagg attgacatca aaaccatttt cttcaagaat attgatgaca aatcgcttgc
     7921 gcgacactcc gagaaagatt ggatatcctt tctgatgtag tttatccagg tcccgtaaaa
     7981 gaagcagatt ttctttcttg gtcagaccaa agccaattcc tagatccaac aggatatttt
     8041 cttgtgcgat tccagcttga tttgctctcg ctagggctcg ttcaaagaaa gtctccatca
     8101 agtcttcgat tggcattttt tcaaagtcag ctaactcttc ctctgtaaaa gcttgaccaa
     8161 caccgaaatg aggaaatatg agcgagctag gatgctgagg tcgcgccatg actggattaa
     8221 acataatgac tactttcgca ccagccttag caaccacatg ggccattttc tcatcaccca
     8281 taagaccagt gatatcattg actagattgg caccagcagc caaagcagct tctgctacct
     8341 gactcttcca agtatcaata gagatgagga catcactttc cttgcgaatc gctttaatca
     8401 ctggaacaac acgctggatt tcctcttcta tctcaacctc aacatagcta cttcccggcc
     8461 gagtcgattc tccgccgata tctagcatac tagccccttc tgctatcaat ttacgagcct
     8521 gctggagtgc ctgctcaaga gcaaaaaatt gaccaccatc cgaaaaggaa tctggggtta
     8581 cattgataat tccgcaaata gctgtctttg catgatttac tttagttgac atatcggtca
     8641 ctccctcaag gcttttcacc atattatttc tctattttac cataaaaaga aaaagatggc
     8701 cacgattgca ttcatctttt tcccagtaga aacatgtaag caattgtcaa aaatcttaaa
     8761 cagaaatccc taatgtccga ctcatgatca ccacaagagc caacaaacag aaagcaaccc
     8821 cattcacaat catgtgaagc aagatagaca tttccaaacg ttgggtcttg taagccgtcc
     8881 aagacagaac gatcgacata cctccataaa tcaataaaga aggtaaatta cttggttgat
     8941 gcaataaagc aaacacaacc gtaccgacta caaatcccaa gttctctttt cctcggaaaa
     9001 tctttttagg aacaatccca cggcacaaga tttcctcaca aattggagca agcaaggcta
     9061 gcaagaaaaa gctagaaatc aaagaactat tttgaaccat atcgttaatc tgagactggt
     9121 tccctgttgt cgtctcattt gacagttgca acaagatgga accaagtata tttgaacaga
     9181 taataactaa ataactcaag cccaaacgtg ctagatcctt agctctaaaa aaagaaaaat
     9241 taaaacttgc taattgcgtt ttacgagctc caataataaa tagcgctaaa accacaattg
     9301 aaataccagc aactatcagt cctgactgta acagtggtac ttcatttaaa tttaaaatgg
     9361 aagtaaccgc taaaggaatc tgatatacaa ttgccgctag caaaaagacc aaaatccagc
     9421 ctgcacgatt caacaattct ttccacatat ttttctcttc catcattcat ctcctagact
     9481 ttgtattaaa aaaaggggga aatccccctg ggtaattata ttataaggcc atgctgatgt
     9541 agttaaggat aaacaaggca tccaaaatcc aaatcatgac atgaacatct ttagcttgac
     9601 ctttgacaag cttagtcaaa gtgtaagtca agaaaccaac tgcaatccct tgagtgatag
     9661 agtagctgaa tcccataaag atagatgtga agaaagcagg aaccgcttca gacatatcgt
     9721 cccaatggat atttttcaag ctagccaaca tcataatccc aacgataatc aagattggag
     9781 ctgtagcggc tgttggtacg atcgctagaa gtggactaaa gaagcttgag atcgcaaaac
     9841 agattgccac gaccaaggct gtcaaaccag tacgtccacc tgcaccgata ccagcagcag
     9901 actcaatata agtcgttacg tttgaagtac ctgcgatggc accaattgaa gtaccaatca
     9961 agtcagagta aagagccttg tccaacttaa ctgattggtg attttcacca tttgtcgcta
    10021 cgataccaac tttttcacct gtaccgatca aggtaccaat tgtatcaaaa atatctgtca
    10081 atgagaaggc aagaatagcc attagagttt caggtaagcg agctgtatct gaaatcaaag
    10141 ctcccaaacc ttctgaacca agagctgcac caaagactgt cttcaagtct tcaaaggctg
    10201 caccaacatg gttattagca aaatcgatac tagacaaatc taccaaacca actgcgatag
    10261 caagaacagt tgttgtcaag atagaaagga taattccccc tttaatccct ttaataacaa
    10321 agaagatagt aatggcaagt cctgcaagag ccaccaaaac agctggatta ttaaagctga
    10381 ccaatcctgg aaccgctgaa gagtttgctg caatcgttgc ttgggctttg tcagcccctt
    10441 ctcctacaac aatatagttt cctggatcaa tcgtgaattt caaaagtcca gcattcttaa
    10501 tccccacgta ggcaaggaag acaccgatac cagctgaaat agctgagcga agggcgtttg
    10561 gaatcgattc aatgatcatt ttacgaacat ttgtcaaggt aataatcaat gagatgaccc
    10621 cacagatgaa gaccatagct agggcttctt gccaagaata accgagccca aatacaactg
    10681 taaaggtaaa gaaggcattg agtcccatac ctggtgcttg ggcgtaaggt aagttagcgt
    10741 agaaggccat catcaaggta ccagcaactg caccgataat cgttgcgagg aatacaccct
    10801 gagcaggcat tcctgtttgc gaaagaattt gtgggtttac aaagagaata tagctcattg
    10861 caaagaaagt tgttaaacca gcgagaacct ctgtacgaac gtctgtacca ttctctttta
    10921 gtttaaataa tttgtccatt atcaatctcc ttttaatttt tacgaacaat aatagaatta
    10981 tatcatttat cattcacttt ttcaatatct ttgtttgatt tatttaagaa tttgatggaa
    11041 ttttattttt tgtttggaat ctgtttttct gcctgctttt tgttataata gtagacatct
    11101 tatctgaaaa gacactagaa aggtccatat gacaaaaaaa attattgcag ttgacctaga
    11161 tggaaccttg ctcaactcag acagtcaaat ttctgacttt accaaaaaaa ccatcaaaaa
    11221 aattactgaa aaaggccatc aggttattat tacgacaggt cgcccttacc gtatgtcaaa
    11281 agatttttac cgtgaactta gcttagacac tcctatgatt aatttcaacg gctccctcac
    11341 tcatttacca gatcaagttt gggactttga aaagtgttta actgtagaca aaaaatatct
    11401 gctagatatg gttcgacgtt cagaggacat tcaagccgat tttatcgctg gagaatatcg
    11461 taaaaaattc tacattacaa atcctaatga agaaattgcc aatcccaaac tatttggtgt
    11521 agaagctttc caacctgaag atcaattcaa gcctgaatta gtgaccaagg atcctaactg
    11581 tattctcttg cagaccagag ccagtgacaa atatgccctg gcaaaagaaa tgaacgcctt
    11641 ctaccagcat caactttcta ttaatacttg gggtggtccg ctcaatatcc ttgagtgtac
    11701 cccaaaaggt gtcaataagg ccttcgcctt agactacttg ctcaaggtaa tgaatcgtga
    11761 caaaaaggat ttgattgcct ttggagatga acacaacgat accgaaatgc tcgcttttgc
    11821 tgggaaaggt tatgcaatga aaaatgccaa cccagagcta ctctcctatg cagatgagca
    11881 aatcgccctg accaacgacc aagacggggt tgccaaaacc ctacaagact tattcttata
    11941 acctatactg atactcaatg aaaattaaag agcaaactag gaagctagcc gtaggttgct
    12001 caaagcactg ctttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa cactgttttg
    12061 aggttgcaga tagaactgac gaagtcagta acatatatat acggcaaggc gaagctgacg
    12121 tggtttgaag agattttcga agagtatgac cagcttgcaa gctggtcttt gtgttctttt
    12181 cacagtctca tcaaccagtt aatcgattgt tctatttttt gcttccaaaa ccttggaaaa
    12241 ggctttcttt tgtgatatac ttcacatata aaaacaagag agctcgtcac actcgactct
    12301 gttggtacaa aatcaatcca gtcctgtttt tcctgctctc agttaatatc aaggaggata
    12361 gctatgaaag ctgttgttgt aaatccagaa agcactggtg ttgctgttga agaaaaagta
    12421 ctccgtccac ttgaaactgg ggaagcactt gtagaaattg aatactgtgg tgtttgccac
    12481 actgacctcc acgttgctca tggtgacttt ggtcaagtac caggacgtgt tcttggccac
    12541 gaaggtgtcg gtatcgttaa agaaattgcc ccagatgtga aaagccttaa agtcggtgac
    12601 cgcgtcagcg ttgcttggtt ctttgaagga tgtggtactt gcgaatactg tacaactggt
    12661 cgcgaaaccc tttgccgtac agtaaaaaat gctggctact cagtagacgg tggtatggct
    12721 gaacagtgta tcgtaactgc agactatgct gttaaagttc ctgacggact tgatccagcc
    12781 caagcctctt ctatcacatg tgctggtgta acaacctata aagctataaa agaagcaaaa
    12841 gttgaaccag gccaatgggt tgtcctttat ggtgctggtg gacttggtaa cctcgctgtt
    12901 caatacgcta aaaaagtatt caatgctcat gttatcgcag tcgatatcaa caacgacaaa
    12961 cttgccctcg caaaagaagt aggtgctgat gtcgttatta acggcctcga agttgatgat
    13021 gtaccaggac tcatcaagga aaaaactgat ggaggagctc attcagctgt cgtaactgct
    13081 gtgtctaaag ttgccttcaa ccaggctgtt gactcagttc gtgccggtgg tcgcgtcgtc
    13141 gctgttggtc ttccttctga aatgatggaa ctcagcatcg tgaaaacagt tttagatgga
    13201 atccaagtca tcggttctct tgtaggaact cgtaaagact tggaagaagc cttccaattc
    13261 ggtgcagaag gtttagtagt cccagttgtt caaaaacgtc cagtagaaga tgctgttgcc
    13321 attttcgacg aaatggaaaa aggtcaaatc caaggacgta tggtactcga cttcacccac
    13381 taatctaata gaaacctatt ttaaaagttg gaagcttctt ccaacttttt tatattaaat
    13441 ttttaaataa gaattcaaga ttactcctca aaaactcaac atatcaacat acaaaaataa
    13501 aattttttta attttttatg ataaatagtt gcttttttta tgaaaacggt ttatacttaa
    13561 acagtcttaa agttttctta aatgaaaact aaaacaaaaa aggaggaatg acaataatga
    13621 gtatcggaat cattattgcg agccacggcg aatttgctgc gggtattcat cagtcaggat
    13681 ctatgatttt tggtgaacaa gaaaaggttc aagttgtgac ctttatgcca aatgaaggtc
    13741 ctgatgatct atacgctaag tttaataacg ctgttgctgc atttgacgca gaagatgagg
    13801 ttctagtttt ggctgacctt tggagtggtt ctccatttaa ccaagctagt cgcgtgatgg
    13861 gagaaaatcc tgagcgtaag tttgccatca tcacaggact taacttaccg atgttgattc
    13921 aagcctacac agagcgcctc atggacgctg ctgcaggtgt agaaaaagtc gctgctaata
    13981 tcattaaaga agccaaagat ggcatcaaag ctcttccaga agagctaaat ccagtcgaag
    14041 aagttgcaag cgctgcagct gctccagttg cccaagctgc tatcccagaa ggaactgtta
    14101 tcggagacgg taaattgaaa atcaatcttg cccgtcttga cacacgtcta cttcacggtc
    14161 aggttgcaac tgcttggact ccagattcaa aagcaaatcg tatcatcgtt gcttcagata
    14221 acgtggctaa agacgacctt cgtaaagaat tgattaaaca agcagctcca ggtaatgtca
    14281 aggctaacgt ggttccaatt caaaaactga ttgagatttc aaaagaccca cgttttggag
    14341 aaacacatgc ccttatcttg tttgaaacac ctcaagatgc ccttcgtgcc atcgaaggcg
    14401 gcgtgccaat caagactctt aacgttggtt ctatggctca ctcaacaggt aaaacattgg
    14461 tcaataccgt tttgtctatg gacaaagaag atgttgctac atttgaaaaa atgcgtgact
    14521 tgggtgttga atttgatgtc cgtaaagtac caaatgattc taaaaaagat ttgtttgact
    14581 tgattaacaa agccaatgtc aaataagcca ttatttatga aaggatttta aacatgtcta
    14641 ttatttctat ggttttagta gtcgttgtag ccttctttgc aggtcttgaa ggcatcctcg
    14701 accagttcca atttcaccaa ccacttgtag cctgtaccct tattgggctt gtaacaggtc
    14761 acttggaagc agggattatc ctcggtggat cgcttcaaat gattgccctt ggttggtcaa
    14821 atatcggtgc tgctatcgct cctgatgctg cacttgcttc tgtcgctgct gccattatca
    14881 tggttcttgg tggtgacttt accaagacag gtatcggtgt tgcccaagcg gttgctatcc
    14941 ctcttgccgt tgctggtcta ttcttaacta tgattgttcg tacaatttca gttggattgg
    15001 ttcacactgc tgatgctgcc gctaaaaaag gtgacttcgg cgctgtggag cgtgctcatt
    15061 tcattgcgct acttttccaa ggacttcgta tcgcgcttcc tgcagctctt ctccttatgg
    15121 taccaactga aactgtacaa agtatcctta gtgccatgcc agactggctc aaagatggta
    15181 tggctatcgg tggtggtatg gtcgttgccg ttggttacgc catggttatc aacatgatgg
    15241 caactcgtga agtatggcca ttcttcgctc ttggtttcgt tctcgctgct gtgtcagata
    15301 ttactctaat cggattcggt gctatcggtg ttgctatcgc tcttatctac cttcaccttt
    15361 ctaaaactgg tggaaatggt ggcggaggag ccgcaacttc taacgaccca atcggcgata
    15421 tcctagaaga ctactaagat aagaaaggac tgaaaacatc atgactgaaa aacttcaatt
    15481 aactaaatca gatcgtaaaa aagtttggtg gcgttcaacc ttcttacaag ggtcttggaa
    15541 ctttgaacgg atgcaaaact taggctgggc ttatacactc attccagcta tcaaaaaact
    15601 ctatactaaa aaagaagatc aaatcgctgc tcttgagcgt caccttgagt tcttcaacac
    15661 tcatccatac gtagctgctc cagtcatggg ggttactctt gcgcttgaag aagaacgtgc
    15721 taacggtgtg gaaatcgatg acgctgctat ccaaggggtt aaaatcggta tgatgggacc
    15781 tcttgctggt atcggtgacc cagtattctg gtttacagta cgcccaatcc ttggatctct
    15841 cggtgcttca cttgccctta ctggcaatat cttgggtcca ctactcttct ttgttgcatg
    15901 gaacttgatt cgtatgtcat tcttgtggta tgttcaagag attggataca aggctggatc
    15961 agaaatcact aaagatatgt ctggtggtat ccttcaagat atcactaaag gagcttctat
    16021 ccttgggatg ttcattcttg ctgtccttgt tcaacgttgg gtaaatatta aatttgcttt
    16081 cgatgtttct aaagttcaac tagatgaaaa ggcttatatc cattgggata aattgccaga
    16141 aggatctaaa ggtatccaag aagcattcgc acaagtagga caaggattgt ctcaaactcc
    16201 tgaaaaagtt actactttcc aacaaaactt ggatatgttg attcctggat tatcaggact
    16261 actccttact ttactttgca tgtacttact taagaaaaaa gtatctccaa tcactattat
    16321 ccttgccctc ttcgcagtgg gtattgtggc acatgttctt cacatcatgt aatcaagcaa
    16381 ctaaaaagga accaggttct aaaatctgat tccttttttc tatgctttta tactcaatga
    16441 aaatcaaaga gcaaactagg aagcgagccg caggctgtac ttgagtacga caaggcgaag
    16501 ctgacgtggt ttgaatttga ttttcgaaga gtattattct gccaaggctc ccattggatc
    16561 ccaaggtgca agtacgattg gttctgctcc ataggcagct tgttcttctg ctgtcagcaa
    16621 ttccttacga acaacgattt ggtatgtgta ttcgtccatc caagcgtctg atgcaacaaa
    16681 gtaaccatct gtaccgacct tgtctcccca tgagttttca accttccact tggttgattt
    16741 accattttcg tccaagtcaa cacctgtcaa gaccatggcg tgggtcatca agctttcact
    16801 atagtccaaa cgtccagcct tgtcttgagt aagtttaatg tccatgcttg attcaaagtc
    16861 ataaacatct gtcgcaagga tgccagcctt acggttgctg agctggccga catcagaacc
    16921 aaaccaaaca gtctcacctg cttgcatttg ggcaatcgcc aattctttca agcgctccat
    16981 tggaacgttg atgtagcgaa ctgcacggct accaaccaca ttccccaaca tctcaactgt
    17041 gtaagatttt ccgtaaggtt tatcagcagt tggagcattg ataacagaaa cgtagtcttc
    17101 taaaggaaga ttgacatatt tcttgtaaaa ctcttgtggt gtgattcctt tttcactttt
    17161 gtagttgtta tctttatcgc gataagcaaa gtcaaacttg cgtggtggaa gtcctaatga
    17221 catagcaaga aagttaaaga tttcttgcaa gaggtcttct ttcttagctt gaacagtcgc
    17281 ttgatctgca ccagaaacaa gcaaatcacg caagatttga gcatcttgac gaagcaattt
    17341 attaaggatc gcatttagct cacgactgct gctagatgaa acagactcag gataaactga
    17401 cttaggcacg acaccgtatt tttcaaagag ggaaacgacc atatcccatt gaccgccatc
    17461 ttgttgaggt gtttggagta agaagctaac cttgcggcta gtcaattctt ggtctgaagt
    17521 cgcaatgact tgctccaaga accagtttga tttctcatac ttatcccaga agaaagtgtg
    17581 ggcttgtgac aactcaaagt tctccaattt gtattgcgag atgagtttgt ggcggaaggt
    17641 gttgagagcc gcaaacatcc agcaacgacc agacgctttc tggttagtga ccttgtcctt
    17701 ggttaaatcc aatgagaaaa caggtgtgtt gtctacatgg ctttggcgac gttccagagc
    17761 tgcaaaaatt ccgttgtggc tggcagcatt ttcaatcgct tggtatttta catttgcttc
    17821 atagttggca aatagtttat cagtaaatga ttcttgaatc gcgttcatag attcctcctt
    17881 ttattctaca gtctattata actcttttca caaaggaagc aactaaaaaa cataaaaggc
    17941 gaggacattg tccctcacct ttttcgaatt tatactcttc gaaaatctct tcaaaccacg
    18001 tcaacgtcgc cttgccgtac tcaagtacag cctgcggcta gtttcctagt ttgctctttg
    18061 attttcattg agtattaaac taaattaaat aatattagcg cggagaattt ctaattcttc
    18121 cttggtcaag cgacgccatt cccctcgttc caggttctca tctaatacta aagttcccat
    18181 agtcaatcgt tgcaagtcca ccacttcctt gccacaatag cccaccatac gcttgatctg
    18241 atgaaacttc ccttctgcaa tggtcacacg gatttggctt tgattctttt ctgtatctat
    18301 ggatacaagc tccagtatag cgggttgaca ggtaaagtct ttgagaggaa tacccttgat
    18361 aaatgtctcc acatcttctt gggtcatgat tcccttgact tgtgccagat aagtcttgtc
    18421 cacatgacgc ttgggcgaaa gaagaacatg agccagctga ccatcattgg tcaagagcaa
    18481 aagaccatgc gtgtcaatat ccaagcgtcc tactgggaaa acttccttac tccgcgccaa
    18541 gtcatccaac aagtccagaa cggttctgtg cttgggatcc tcagtcgctg agataactcc
    18601 tttgggcttg ttcatcatgt agtagacaaa ctcttcatac tccaacactt gcccatcaaa
    18661 gcgaatctca tctatttttt catcaatctg caatttagct gatttttctt ttttaccatt
    18721 tacagtcacg cgcccagcct tgagcaagtt tttgacctca gtccgacttc ccaccgcaca
    18781 ggcaactaaa aatttatcta atctcataga actattatat catatcaaaa ggaggctagt
    18841 acaatgacca acctcctttt cgtttcatac tcttcaaaaa tctcttcaaa ccgcgtaaac
    18901 gtcgccttgc cgtatatatg ttactgactt cgtcagttct atctgcaacc tcaaaacagt
    18961 gtttttagct gacttcgtca gttctatctg caacctcaaa gcagtgcttt gagcaacctg
    19021 cggctagttt cctagtttgc tctttgattt tcattgagta tcagatttag gaaattaact
    19081 tcctcgtctc caaaaaatag ctaaaacaat catggctcct aaaatcgctg ggataatagc
    19141 tgttcctgat aaaactggcc cccaagttcc aaagagcaag tgacctagaa aggctccgat
    19201 ccaaccgaga aacatttttc caaaacatcc cattcgctct ccacgattgg tcatagcacc
    19261 tgctaaaaat cccactagga gaccaacgaa catacttcct aacatattat ctccttaatt
    19321 tgcccaattc ccattacgga aaagaggtac tcgcgttcca tcctcacgaa taccatcgat
    19381 atccatttgg ttagaaccaa tcataaagtc tacgtgaaca tctgaacggt taagccctgc
    19441 agcttcaagc tcctcttcgc tcatctccgc tccatcaaca acgctagtcg cataggctgc
    19501 accgatagcc aagtggtttg acgcattttc atcgaaaagg gtgttaaaga aggtaatgcc
    19561 tgactgagaa attggacttg gatctggtac caaggcacat tcacccaagg cacgcgcacc
    19621 cgcattttca aagacaaggt ctttcataac ctgatcaccc ttctcagcag tgatatctac
    19681 gatttgtcca tccttaaagg ttaccttaat accttcgatg atatttccgt tgtagctaag
    19741 cggttttgta gaagtgacat aaccatctgc acgacggaag tcaggcgctg tgaagacctc
    19801 ttctgtcggc atatttggca agaatccttc gccctgtgca ttgacagcac cagctgattc
    19861 ccaaacgtgg ttctttggca aaccaagtgt taaatctgtt cctggcgctg tgtagtgaag
    19921 ggctgaaaat tgctccttat taagcatatc ggccttgctc ttcaagatag ctgcatgctc
    19981 ttcccaggcc ttaacaggat ctgcttcgta gacacggcaa gttttgaaaa tttggtccca
    20041 aaggaaatca actgcttctt cgtcgctcgc agcatttggg aagactttct tagcccactc
    20101 aagtcctgct gcagctgcta cagtccagct aaccttgtta gattgagttg cgatacgcat
    20161 aggcttcatg gcaagtccca tagctttagc agaagctgaa agcttgtcag cgtccactcc
    20221 gttcaaggca cctggatcag atgaacgaac tccaagacgg ctagccttat tctccaagag
    20281 atagttcatc tcagcaatct tgtattctgg cacattgtcc aaacgctcca tcggggcatg
    20341 gagaaatttc tcacggttaa tcacatcatc tgtccactga acgatgacct catgcgcacc
    20401 caaggcataa gcttctttca cgattagatg tgccaattca cgttgctcca catcaataga
    20461 gagagccaaa gtgtgaccag gttgcacgtt aattccgttc gcaaccaaca atttcgcata
    20521 tttttctaga ttttctttaa aatttggtaa aaccatgtaa ttttctcttt tctattttta
    20581 tttttctttc aaagcagaga ataatcccgc taaagcaata gcacctgaca agagtgctgt
    20641 tgatagaaga attgtacgat cagcaagttc taattgatat tcaaatacct tcttagccgg
    20701 cttatcttcc gcaaatattc ttagtttcat gagaaccccc ttaaaataga ttaaaataaa
    20761 tcactgtgac tgcctgtcct cagcaaattt aaaatcaatt ccgacttgtc tactttataa
    20821 accaaaagcc aatctggctg ggtatggcat tcacgaactc cttgaaaatg cttggatgcc
    20881 gtcaatgaat gatcacgata tctggtagga tgttcttttt cttgaaccag aaaattcaaa
    20941 acttcttcta ataattctgc cttcaaacca cgcttcatag ccaacttaaa atctttttta
    21001 aactgtttat gatagcgaat cttaagcacg taagtcctcc atcaaatctg agactgattc
    21061 aaaagactgg ctcatattac gattttagtc taaatccgtt aacgcttgga gcaacttccg
    21121 attttcgtct agtctaacat caaaaggtaa tccctgatat tgaattgcct gacgaaggaa
    21181 aatattaata gctgttgtca tatccattcc cagattacta aacacctgtt gggcctgctc
    21241 cttaacctca ctatccagac ggatgcttat actcatcttt gacatactct caccctcttt
    21301 ccatagacta ttttaacaaa aaagaaagct aatgtaaatc tattggatat acgttagcct
    21361 cttttaatag attattaagc aattttttaa aacaactcat caaacaaact caactggtta
    21421 tcctctggca tatttccaag aatacccatc tcatccatct tttcaaccaa ggttgatgag
    21481 agtccaccac gcttgcgtag ttctgtttta gagaggaatt ctccctcttc acgcgcccgc
    21541 accagttgct tggcaacgtt ctctcccaga ccatccattg ctacaaatgg tgggataagg
    21601 gtatccccgt cgatgaggaa ctctgtcgcc tgactacagt agagatctaa tttaccaaac
    21661 ttgaaacctc gttcccacat ctcattgaca atctcaagag ttgtatagag atcgatttcc
    21721 acattagagg cttcattgtt cttccgtttt tcagagattt cttccattct gcgcttgatg
    21781 gcctccaagc ccgcacccat ggtcttgata tcaaaagcct tagcacgaat ggagaagtaa
    21841 gcacagtagt aataaatagg atggtgaacc ttgaagtaag ctacacgcaa ggccatcata
    21901 acgtaggctg ccgcatgggc cttagggaac atgtacttaa ttttcccaca ggattcgata
    21961 taccactctg gcaccttatt agccttcatg gcttcgatat agccatttct ctcctcttct
    22021 gaaatcttta gccacaaacc cttacgtacc cgttccataa tggtaaaggc catcttaggt
    22081 tccagacccg catgcatgag gtaaaccatg atgtcgtccc gacaaccgat aacagtcgat
    22141 aggtccgcta ttccttgctt aatcagatcc tgagcattcc ccaaccaaac gtcagtaccg
    22201 tgggacagac cagacagctg aagcaattcc gcaaaggttg tcggatgggt ttcgtctacc
    22261 attccacgta cgaaatttgt tccaaactct ggaatcccca acatacccgt aggcgttcca
    22321 atttgttcag gtgttacccc tagcacatca gtcccagaaa agagtgccat cacgccttcg
    22381 tcatccatag gaattttatt agggtcaata ccagacaaat cctgaagttt tcgaatcatg
    22441 gtcggatcat catgtcccag tacatcgagt ttgaggacgt tctcatcgat atcgtggaag
    22501 ttaaagtgag tggtctgcca ttcagccgtg acatcatctg ctggatactg gacaggcgta
    22561 aaatcgtaga catccatgta gttcggaata acaacgattc cccccgggtg ttggcctgtt
    22621 gtccgcttga cacccgccgc tccttgagcg aggcgttcta cttctgcatc acgataaaac
    22681 ttgccataat ctcgctcgta acccttgaca aatccatagg cagtcttggc agctactgta
    22741 ccaaccgttc ccgcacggaa ggcatattct tcaccaaaga tatcacgcac atccaagtgg
    22801 gcgctaggct gatcttctcc cgagaagttc aagtcaatat caggaacctt atccccatca
    22861 aaaccaagga aggtctcaaa cggaatatcc tgtccgtttt tactgagttt gtgaccacag
    22921 tttggacagt ccttatgggg catatcaaat cctgaaccgt acgaaccatc tgtgataaac
    22981 tcactgtact gacactgacc acagacatag tgaggagaga gaggattgac ctccgtaatc
    23041 ccaatcatgg tcgcaacgaa actagatccg acagacccac gagaaccaac caaataaccc
    23101 cgttcattag aacgttgcac cagcatctgc gatgccaaat aaatcacagc aaatccattc
    23161 cccagtatgg atgttaattc tttttcaatc cgcaaatcaa caatatctgg cagcggattc
    23221 ccataaatct caaaagcttt cttataggtc aactcagcaa ctgtttcttc agccttgtcg
    23281 atgaaaggcg tatacaagtc acccttaacg acttcaacgg gttcaaatat ttctgccaag
    23341 gcattggtgt tttcaataac cagtttacga gccagttcct ctcccaaaaa ggcaaattca
    23401 tccaacatct cattagtcgt tcgaaaatga gcctttggaa gtggcgctgg ttgggcatgt
    23461 tcaccatgac cgatagttcg gttaatcatc gcaccctgtc ccaaactacg gacaataatt
    23521 tcacgataaa tctcttcttc cggttcgata tagtgaacat ttcccgtagc cagaacaggc
    23581 ttgccaaggc gatctccaac ctctatcaaa ctcttgataa tggtctggag ttcctccata
    23641 tccttgacct gctctttggc aatcaagggc gcatagatag ccggtggcat gacctcgata
    23701 aagtcataat acttggccac ctcaacagcc gcatccacac cttgagaaac gaccacgtca
    23761 aaaacttcac cctctgaaca ggctgaacct aaaatcaagc cctctcgatg ggcatctaga
    23821 accgttctcg gaatccgtgg cactccttca aaatacttgg tattagacaa ggaaaccagc
    23881 ttaaagatat tttttagacc tacctgattc ttgacataga tggtcgcatg cttgatccga
    23941 gcttttttgt aagaatctgg actgattaga tcaatgttga gtctatctaa atcggtcaca
    24001 ccatgttttt ctgctacctc tttgataaag atgaaaagca gacgaccagt cgcttccgca
    24061 tcgtagttgg ccatgtggtg atgttccaag gccacaccaa aacgcttggt caaaggcccc
    24121 aaaccatggc gtttatactc aggatagagg tttctagcaa actccagcgt atcaataact
    24181 ggctgactaa tctttggaag atcatgacgc tcataattag cattcataaa gccaacgtca
    24241 aaggtagcat tgtgggcaac taggaccgta tccttgcaaa attcttggaa ttcttgcaaa
    24301 acttgttcta gtggtttggc atttttgaca tgatcatctg taattccagt taactctgta
    24361 gtaaaagctg acaagggatg cccaggattg ataaattcat caaattcagc aataacattc
    24421 cccttgtaca tcttagaggc cgcaacctga atcaagtcat tatagatagc tgaaagtccc
    24481 gtcgtttcca cgtcaaagac cacgtaggtt gcttctgaca agtccatctc cacttcgtta
    24541 tagacgatag ggacacggtc ctccacgata ttggcttcca ttccatagat cagctggatt
    24601 cccgctttct tagccgcctt atagccatgt ggaaaggact ggacattccc atggtccgtg
    24661 atagcaaccg ccttgtgtcc ccacttagca gctgttgcaa caatctcttc gacctctggc
    24721 aaagcatcca tagtcgacat gttagtatga gcatgaaact caacccgacg ctcaccttct
    24781 ggcatcaaat ccttccgctc atagtgaaca acttcctgca gatcctgtac gttcatagtc
    24841 aaatcgcgtg tgaagttatt catctccaca ttccctcgaa ctcggagcca agaattcttc
    24901 ttgatgaggt caaacttctg ggcctcttcc tcgtttttaa cccacttttg catagaaaaa
    24961 cttgaagtat agtccgtcat tttaaagttg attaaaacac gacctgttct agtcactttt
    25021 tgctccacat caaaaacaac cccttcaaat accagacgat tttcctctgt cgtcacttcg
    25081 atcataggag taatctccgc cttatccagc ttgggtttag ctgcagcttt tttcgcttga
    25141 aaatcaaaga ctggtttctc ttccgctgga ggaggtgcca tctgctccag ttgttccata
    25201 gcacggagcg cttcctcatt ggcagcttga acaatctgct cattttcagc atgaaaggcc
    25261 tcttcctgct cttgggtcag gacatcattc ttctcgactt gacagttaaa agttggaaaa
    25321 ccaaactttt caagttgttt ggctaaatta ggaagatgat tcttcttaaa atgttcctta
    25381 tcaatcgcct cagatccttc aataaatagc tgattaccct cagcacgaac ttgcaaattt
    25441 tgataaaggg acttaaaacc ttgactagca catggacctt cagagaaagc ctccctatag
    25501 taggactgca agagctgatt tgaaaattct tgagaccgag ccttaatttc aaaaacagct
    25561 ttattgcctg tcttagaaaa ttcttcgctc aaacctttct ttaattctaa aaagatttca
    25621 atcggtaaaa tattagaaaa tacgaaatga aactcccata ccttactaat tttatgaacc
    25681 acaactcgct caatattggc ctgtgctaaa gcaggagcct gtctcatttc agcaggcatc
    25741 cccaattgat tcatcaaaat ttcaaaacta tttgacattc attttcctca cattattctt
    25801 ctactatttt accatattta gaggtatttt ctaaagacaa aaggaagcca ctaagtgact
    25861 tccttctaga gtgaggacgg attagtcttc acctttattt ttcttaataa tttcttcttg
    25921 tactgactta ggtacatctt cgtagtggtc aaataccatc atgaatgtac cacgtccttg
    25981 agatgcagaa cgaagaactg ttgcgtaacc gaacatttca gcaagtggaa cgtaagcacg
    26041 aacgatttgg ctgttaccgt gtgcttccat accatctaca cgtccacgac gagcagttac
    26101 gtgacccata acatcaccaa ggttttcttc tggaacagtg attgttacaa gcatcattgg
    26161 ttcaaggata gctggttgtg ctgatttagc agcttcttta agggaaagtg aagccgcaat
    26221 cttgaaggca gtttcagatg agtcgacatc gtgatatgaa ccatcataaa gcttagcttt
    26281 aacgtcaacc attgggtaac ctgcaagaac accgttagcc atagattcta ccaaaccttt
    26341 ttcaaccgct gggataaatt cacgaggaac cacaccaccg acgattgcgt tttcgaattc
    26401 gaatccttta ccttcttcgt ttggagtaaa ttcaatccac acatcaccga attgaccttt
    26461 accaccagac tgacgtttga agaatccacg tgcttgggta gaagcacgga atgtttcacg
    26521 gtaagatact tgaggcgcac ctacgttcgc ttcaactttg aactcacgac gcatacgatc
    26581 aacaaggacg tcaaggtgaa gttcacccat acctgagata actgtttcac cagtttcaac
    26641 gtttgtttca acgcggaatg ttggatcttc ttcagccaat ttttgaaggg cgatacccat
    26701 cttgtcttgg tcagctttag attttggctc aaccatcaat tggataactg gttctggaac
    26761 gttgattgac tcaaggatga ttttagattt ttcatctgtc aatgagtcac cagttgtagt
    26821 atctttcaaa ccaacggcag cagcgatatc acctgagtaa acagtgtcga tttcttgacg
    26881 gctgttagcg tgcatttgaa ggatacgtcc gatacgttca cgtttacctt tagaagtatt
    26941 caatacgtat gaacctgatt gaagaacacc tgagtaaaca cggaagaatg tcaaacgacc
    27001 tacgaatggg tcagtcatga tcttgaaggc aagagctgca aatggctctt cgtcagatgc
    27061 tggacgagtt tcttcagcgt ctgtatctgg gttaatacct ttgattgctg ggatgtcaag
    27121 tggacttgga aggtagtcga taaccgcatc aagcatcaat tgaacacctt tgtttttgaa
    27181 ggctgaacca cacaatactg ggaagaattc aacgttgata gtcgctttac ggataccagc
    27241 tttcaattct tcgttagtga tttcttcacc ttcgaggtat ttcatcatca attcttcgtc
    27301 agtttcagca actgcttcaa tcaatttttc acggtattct tgagcttggt caaggtattc
    27361 agctgggatg tcttcttcaa ggatatccgt accaaggtcg ttagtataga tttcagcttt
    27421 catcttgatc aagtcaatga taccacggaa gtcatcttca gaaccgattg gcaattggat
    27481 tgggtgtgca tttgcttgaa ggcgatcgtg aagtgtgctt acagagtaaa ggaagtcagc
    27541 accgattttg tccattttgt tggcaaatac gatacgtgga actccgtact cagttgcttg
    27601 acgccaaact gtttcagttt gaggctcaac acctgattgt gagtcaagaa cggtaaccgc
    27661 accatccaat acacgaagag aacgttgtac ttcgattgtg aagtccacgt gtcctggtgt
    27721 gtcgatgatg tttacgcggt ggttgttcca ttgagctgtt gtcgcagcag atgtgatcgt
    27781 gataccacgt tcttgctctt gctccatcca gtccatttgt gacgcacctt cgtgagtttc
    27841 accgattttg tggattttac cagtgtagta aagaatacgc tcagtagttg ttgttttacc
    27901 ggcatcgacg tgagccatga taccgatatt acgagttttt tcaagtgaaa attcgcgtgc
    27961 catgaggttt gtttctccta tttatttttg atttctattc tattataaca cgattttaat
    28021 aaaaacggat aggcaggacc tacccgttct caatgttttc atgctattgt tggtttcaac
    28081 ttacgagatg gtaagctgag ttatagctaa tactaatcga tttagctaat ttgaacccgg
    28141 gctaaagtta gttagccgat gtgagctgga acgggatgct gcgtgaaaaa gataaaactc
    28201 cttgtattcg ttgaatactg cgtcagtttc ctattttcac cttgcatcct taccgtgcct
    28261 agcatcatga tttagttgag ctcaagctaa aagtctggag aaaaagatga atctcattgg
    28321 ttgcaaccgc aacctgcttc gattccctat tttctctggg actttcttaa cgctttcgca
    28381 tcctatctta ccaacggaag tgtgcgaatg cacggttagc ttcagccata cggtgagtat
    28441 cttcacgttt cttaacagct gcaccagtgt tgttagcagc atccaagatt tcttttgcaa
    28501 gacggtcttg cattgtgtgt tcaccacgaa gacgagcgat tgttaccaac caacgaagtc
    28561 caagtgttgt acgacgttct ggacgaactt caactgggac ttggtagtta gaaccaccaa
    28621 cacgacgtgc acgtacttca agtacaggca tgatgttttc catagctgtt tcaaatactt
    28681 caagtgcatc gttgccagta gcttctttga tttgctcaaa agcaccgtaa acgattgaag
    28741 cagcagtacc acgtttacca tcaagcataa cgcggttgat aagacgagta actagttgtg
    28801 aattgtatag cggatctggc aatacgtcac gttttggagc tctattttta cgactcattt
    28861 ctctttatcc cctttcctta tgcttttgga cgtttagtac cgtatttaga acggccttgt
    28921 ttacgatcgt taacacctgc agtatcaagt gcaccacgga cgatatggta acgtacccct
    28981 ggaaggtctt ttacacgtcc accgcgaaga agcaccacgc tgtgctcttg caagttgtgt
    29041 ccgatacctg ggatgtaggc agtaacttcg ataaggttgc tcaaacgtac acgagcgaat
    29101 ttacgaaggg ctgagttagg ttttttaggt gtcattgttc caacacgagt tgcaacacca
    29161 cgtttttgtg gtgaagaaac gtttgtttga acttttttat gactattgta accaacgttc
    29221 aaagctggtg atttagattt ttctactttt gatttacgcg gtttgcgaac caattggtta
    29281 attgtaggca tctacattct cctgtgtttt tttatttttg gtgatgatac acttggtgac
    29341 agatatcatc tgtgtgtact tttgcaacat ttgtcagcac gtccctgtac actcttgaga
    29401 gaccaaaagt aaaaagtacc gtctattatt gtaacaaaat tttcccttgc ttgtcaagat
    29461 atttttcttt tttattggta atgttagaga tcaatttctg aataaaaata gctgggaaca
    29521 catcatcttc ctaataatac ggcttggctt atgtgacatt tttatctccg tcatttatac
    29581 tgatatataa aatagtacag aaaacttctc aaaagtaaga atttttttca caaatcaaag
    29641 aaaacgcttg catattgttt tactacatgc tatactgata tagaaattta ttgattggag
    29701 aatcattatg agaaaaacac catctcacac tgagaaaaaa atggtctaca gcatccgttc
    29761 cctcaaaaat ggaactggtt ctgtccttat cggcgcaagc cttgtcttgc ttgccatggc
    29821 tacaccaact atctcatccg acgaaagtac accaaccact aacgaaccca acaacagaaa
    29881 tacaaccacc cttgcccaac ctcttactga tacagcagct ggctctggta agaacgaaag
    29941 tgatatttct tcacctagaa atgcaaacgc ttccctagag aaaacagaag aaaaacctgc
    30001 aacagagcca actacttcta ctagtccagt aacaactgaa actaaggcag aagagcccat
    30061 cgaagataac tacttccgta tccatgtcaa aaaacttcct gaagaaaaca aggatgctca
    30121 aggactatgg acttgggacg atgttgaaaa accatctgaa aactggccaa acggagcttt
    30181 gtccttcaag gatgccaaga aagatgacta cggctattac ctagatgtca aattaaaggg
    30241 agaacaagcc aagaaaatta gcttccttat caacaataca gctggaaaaa atctaaccgg
    30301 cgataaatct gtagaaaaac tagttccaaa aatgaacgag gcttggttag accaagatta
    30361 caaggttttc tcttacgagc cacagcctgc aggaactgtt cgcgtcaact actaccgcac
    30421 agatggcaac tatgacaaga aatctctctg gtactgggga gatgtaaaaa atccaagtag
    30481 cgctcaatgg cctgacggaa cagactttac ggctacaggc aaatatggcc gctatatcga
    30541 cattcctctt aatgaagccg caagagaatt tggattttta ttactagatg agagcaaaca
    30601 aggagacgac gtgaaaatcc gtaaagaaaa ttataagttc acagatttga aaaatcatag
    30661 ccaaattttc ctaaaagacg atgatgaatc gatttacaca aatccatact atgtccatga
    30721 tatccgtatg acaggagccc aacacgtagg cacttctagc attgaaagta gcttttcaac
    30781 acttgtcggt gctaaaaaag aagatatcct caaacactcc aacatcacta atcacctagg
    30841 aaacaaggta actattaccg atgttgcaat cgatgaagct ggtaagaaag tgacctacag
    30901 cggagatttc tctgacacaa aacatcctta tactgttagc tacaattccg accaattcac
    30961 taccaaaaca agctggcgcc tgaaagatga gacatacagc tatgatggca aactgggagc
    31021 tgacctaaaa gaagaaggaa aacaagttga tttgaccctt tggtcaccaa gtgctgataa
    31081 ggtttctgtt gttgtctacg acaagaatga ccctgacaaa gtagttggaa ctgtcgctct
    31141 cgaaaaaggg gaaagaggaa cttggaaaca aactctagac agcacaaaca aactcggaat
    31201 cacagatttc actggctact attatcaata ccaaatcgag cgtcaaggta aaactgttct
    31261 tgcactcgat ccttacgcta aatctcttgc tgcttggaat agcgacgatg ccaaaattga
    31321 cgatgcccat aaagtggcta aagccgcctt tgtagatcca gctaaactcg gacctcaaga
    31381 cttgacttat ggtaagattc acaatttcaa gactcgtgaa gacgccgtta tctacgaagc
    31441 tcatgtgcgt gacttcactt cagatcctgc cattgcaaaa gacttgacca aaccatttgg
    31501 gacttttgaa gccttcattg aaaaactaga ctatctcaaa gacttgggtg taacccatat
    31561 ccagctcctt ccagtcttgt cttactactt tgtcaatgaa ttgaaaaacc atgaacgctt
    31621 gtctgactac gcttcaagca acagcaacta caactgggga tatgaccctc aaaactactt
    31681 ctccttgact ggtatgtact caagcgatcc taagaatcca gaaaaacgaa tcgcagaatt
    31741 taaaaacctc atcaacgaaa tccacaaacg tggtatggga gctatcctag atgtcgttta
    31801 taaccacaca gccaaagtcg atatctttga agacctagag ccaaactact accactttat
    31861 ggatgccgat ggcacacctc gaactagctt tggtggtgga cgcttgggaa caacccacca
    31921 tatgaccaaa cggctcctag ttgactctat caaataccta gttgatacct acaaagtgga
    31981 tggattccgt ttcgatatga tgggagacca tgacgccgct tctatcgaag aagcttacaa
    32041 ggctgcacgc gccctcaatc caaacctcat catgcttggt gaaggttgga gaacctatgc
    32101 cggtgatgaa aacatgccta ctaaagctgc tgaccaagat tggatgaaac ataccgatac
    32161 tgtcgctgtc ttttcagatg acatccgtaa caacctcaaa tctggttatc caaacgaagg
    32221 tcaacctgcc tttatcacag gtggcaagcg tgatgtcaac accatcttta aaaatctcat
    32281 tgctcaacca actaactttg aagctgacag ccctggagat gtcatccaat acatagcagc
    32341 ccatgataac ttgaccctct ttgacatcat tgcccagtct atcaaaaaag acccaagcaa
    32401 ggctgagaac tatgctgaaa tccaccgtcg tttacgactt ggaaatctca tggtcttgac
    32461 agctcaagga actccattta tccactccgg tcaggaatat ggacgtacta aacaattccg
    32521 tgacccagcc tacaagactc cagtagcaga ggataaggtt ccaaacaaat ctcacttgtt
    32581 acgtgataag gacggcaacc catttgacta tccttacttc atccatgatt cttacgattc
    32641 tagcgacgca gtcaacaagt ttgactggac taaggctaca gatggtaaag cttatcctga
    32701 aaatgtcaag agccgtgact atatgaaagg tttgattgcc cttcgtcaat ctacagatgc
    32761 cttccgactt aagagtctcc aagatatcaa agaccgtgtc cacctcatca ctgtcccagg
    32821 ccaaaatggt gtggaaaaag aggatgtagt gattggctac caaatcactg ctccaaacgg
    32881 cgatatctac gcagtctttg tcaatgcgga tgaaaaagct cgcgaattta atttgggaac
    32941 tgcctttgca catctaagaa atgcggaagt tttggcagat gaaaaccaag caggaccagt
    33001 cggaattgcc aacccgaaag gacttgaatg gactgaaaaa ggcttgaaat tgaatgccct
    33061 tacagctact gttcttcgag tctctcaaaa tggaactagc catgagtcaa ctgcagaaga
    33121 gaaaccagac tcaacccctt ccaagcctga acatcaaaat gaagcttctc accctgcaca
    33181 tcaagaccca gctccagaag ctagacctga ttctactaaa ccagatgcca aagtagctga
    33241 tgcggaaaat aaacctagcc aagctacagc tgattcacaa gctgaacaac cagcacaaga
    33301 agcacaagca tcatctgtaa aagaagcggt tcgaaacgaa tcggtagaaa actctagcaa
    33361 ggaaaatata cctgcaaccc cagataaaca agctgaactt ccaaatacag gaatcaaaaa
    33421 cgaaaacaaa ctcctatttg caggaatcag cctccttgcg ctccttggtc tcggtttctt
    33481 actaaaaaat aaaaaagaga actaaactag ccctcctata gaaaaatccc ccaagcatta
    33541 tagctcgggg gattaatttt cgtacaatat ttgttgtcct aataaacttg attaggattt
    33601 tttattaagc ctctttcata gcaaaataag ctcgtacttt gggtgcaact tgtgttccga
    33661 agagttcaat agctctcaga acctggtcat gaggcataga accaagcggt agatgaagca
    33721 tgaagcggtc caatcctaaa tcctctatca tgcgaatcaa tttttcggcc acctgatctg
    33781 gattgccaac aaacatggcg ccatttggcc ctacctgctc caaatattgc tcataacgca
    33841 attcctgcca gtgcggacgg tctttggaaa tagcatccac cacttgctta gtcggatgga
    33901 aataatcttt caccgcctgc tcaccatctt ccgcaatcca cccccaagaa tgggctccca
    33961 ctttcaagtc tttgtcagca tgacccgctt cgcttccaat ctcacgataa gcctgaatca
    34021 actttttaaa ataacgtgga ttaccaccaa taatagcata tacaatcggt agaccagcct
    34081 gagcaatctt cactgttgat tcgacatgac cacctgtagc tatccacaag ggcaatttgt
    34141 cctgaactgg acgaggataa acttctttac cagcaatcgt ttgagtcaat cgaccttgcc
    34201 agtctaactt ggtcttttca ttgactaact gaagcaagtc taatttctca tcaaaaagag
    34261 agtcgtagtc tttcaagtca taaccaaaca gagggaaaga ttccgtgaaa gagccccttc
    34321 cagccataat ctccgctcgt ccatttgaca aagcatcgat agtggcatac tgttggaaca
    34381 aacgaatcgg gtccatgctt gacagaatgc tgactgcact ggtcaaacgg attttcttgg
    34441 tattgactgc cccagcggcc agaacaatct ctggggctga tactgcaaaa tccgcccgat
    34501 ggtgctcacc aatcccatat acatccaaac caaccttgtc agccagctca atctctgcca
    34561 ccaactggcg aatgcgttca gcatgactgt aagtttgtcc agtcctttca agctccgtta
    34621 tttccccaaa tgttgaaatt cccaattcta ccattgtgat tctccttgtc tatctctgtc
    34681 cttcaatttg aaaaattatt ctaacacgaa tcttaagtac aagcaaccaa tttgctcatt
    34741 agaaaaagcc tagataacta gactttttta gcttattcta ccgttactga tttagcaagg
    34801 ttacgtggtt tatccacatc gaggccacgg tggagggttg caaagtaagc gactaattgc
    34861 gttggtacga ccattgaaat tggtgagagg tatggatgta cggtcgtaag gacgatatcg
    34921 tcggtatctt tggctacatt ctcttctgcg atagtgagga ctttggcacc acgggctgcg
    34981 acctcttgga tatttccacg agtatggttg gcaagaactg gatctgacaa gagagccaaa
    35041 acaggcgttc cttcttcaat caaggcaatg gttccgtgct tgagttctcc tgcagcaaag
    35101 ccttcacact ggatataaga aatctctttg agtttgagac ttgcttccat ggctacgtag
    35161 taatcttgac cacgtccgat gtaaaaggcg ttacgagttg tttcaagaag ttcacgaacc
    35221 ttgacttcaa tggtttcttt ctctgaaaga gttgattcga tagactgagc tacgattgac
    35281 aattcatgaa ccagatcaaa gacttgcgct ttagcattac catttgcttc tccgactgct
    35341 tttgcaagga aggcaagggc tgcgatttgc gctgtatagg ctttagttga tgccacagca
    35401 atttcaggac ctgcgtgaag gagcatggta tagttggctt cacgtgagag ggttgaacct
    35461 ggaacatttg tcactgttaa gcttggaatt cccatttcat tagccttgac caaaacttga
    35521 cgactatccg ctgtttcacc agattggctg ataaagatga agagtggttt cttgctgaga
    35581 agtggcatac tgtagcccca ctcagatgag attccaagtt caactggtgt atctgtcaat
    35641 tcttccaaca ttttcttaga agcaaatcct gcatggtaag atgttccagc tgcaaggatg
    35701 tagatgcggt ctgcgtcttg aacagcctta atgatagcag gagcaaccac tacttgacca
    35761 gcatcatccg tgtaggcttg aatgagttta cgcataacag ttggttgctc atcaatttcc
    35821 ttaagcatgt agtaaggata agttccctta ccgatatctg acaagtcaag ttccgcagta
    35881 tagctagcac gttcacgact gttaccatca tagtcttgaa cttccacgct atcagccttg
    35941 acgattacca actcttggtc atgaatttcc atgtattggt tggtttcacg aatcatagcc
    36001 atagcatctg agcagaccat gttatagcct tctccaagac caatcaaaag tggtgattta
    36061 tttttagcta cgtagatgac ttcaggatct tgtgagtcaa ccaaggcaaa ggcataagaa
    36121 ccacggatga tgtgaagggc ttttttgaag gcttcaagaa ctgagagccc ttcttcttcc
    36181 gcaaattttc caatcaaatg aacggctatt tcagtatctg tctgcccctt gaagtggtga
    36241 cctgcaaggt attcttcctt gatttcaagg tagttctcaa tcaccccatt atgcaccaag
    36301 acaaaacgtt ctgtctcaga gcggtgtggg tgagcattgt cctcagttgg ttttccgtga
    36361 gtagcccaac gagtatgtcc gataccagtt gttccctcaa caccagctgt cttggcagac
    36421 aattctgcaa tacgaccaac agccttcacc aaatggttat cagcaccatc taggacaaaa
    36481 attcccgcag aatcatagcc acggtattca agcttttcaa gcccttgaat caaaatatca
    36541 gttgcatttg tgtttccaac aacaccaaca attccacaca tagtatatac gacacaggca
    36601 agctgtgctt tctccttaaa attggtatag tctaattcat cttttataga atcagcaaaa
    36661 acagtatata cttgtttctt tcacttgtca agagtaaaaa ttggtatagt tcaaattaag
    36721 ctcctgtaag caaaaaactc tgaccgattg ggataatcag tcagagtcct ttttaaaatc
    36781 cattattatc gcttaatttt ttgaaccagt ggcctgattt cttcagacga cgttcttgcg
    36841 tttccaagtc taattcgacc aaaccatagc gatttttata gctgttgagc catgaccagc
    36901 agtcaataaa ggtccaaatc aagtagccct tacagttggc accatcttca atggcacggt
    36961 gaagttcacg aagatgacct tttacaaagt caatacggta atcatcttga atcattccat
    37021 cttgacggaa tttttcttcc ccttcaacac ccataccatt ctcagtcaac atccattcaa
    37081 tattgccata attttccttg atattttggg cgatgtcata aatcccttgc tcataaatct
    37141 cccaaccacg gtgagaattg attttacgtc caggcatcac ataaggctcg taaaaatgtt
    37201 ctggtaagag tggactctct ggatgcttag caaatcgagg agccataaca cgcaaaggtt
    37261 gatagtagtt cacaccaagg aagtccaccg tattatcacg aatgagttcc aactcttcct
    37321 ctgtagcatc aggtaaaaga ccgtgttcat gcaagatttc taccaactcc tgtggataag
    37381 tccccaagac agatggatct aagaaagatt gggcctgaaa aagggccgca atacgagctg
    37441 ccttgacatc agcaggatgc tggctacgtg gataagccgg tgtcaagttg aggacaatcc
    37501 caatcttgga atcaggcaaa agttcatggc aagccttaac agcccggctg ctggccaatt
    37561 gtgtatgata ggctacctta acagctgcct ctgcatccac cttatgtgga taatgggcat
    37621 cataaaaata accaaattct acaggaacga tgggctcgtt aaaggtaatc cattgatcca
    37681 ctaaatctcc ataagtctca aaacaaaaac gagcatagtc ttcataggct gagactgtcg
    37741 ccttattttc ccaaccatca ccatcctctt gaagggcaaa aggtaaatca aaatgataga
    37801 gattgactaa cagacaaatt cctttagcct taatagcctc aaagacctta cgataaaaat
    37861 ccacaccttg agggttgact tttccacagc cttgtggaaa aatccgtgac cactgaatag
    37921 aagtccgaaa gactgtgtga ccagtctcta acaaaagctc aatatcccgc tcccaatttt
    37981 cataaaaagt cgatgtctta tctgaaccaa tcccattata gtaacgattt ggctccactt
    38041 ggaaccagta atcccagaga ttgtctccct taccgtcacc agctacacgt ccttctgtct
    38101 gcggtccaga agtagaggat ccccagacaa aatcctttgg aaatcttagc ataacattta
    38161 cctctttatc tactcatttc tcccattata cagaaaaaac aaggtaaaaa ctagttacat
    38221 tttttccttg tttttcttct gattatagtt tttatttctt gcttaggatt tcaagcgttt
    38281 caagcacgtt atctgcatga acctcaatgg tgtcaccagt tgccttgatc ttaacttcta
    38341 caatgccatc ggccgctttt ttaccaacag tgatacggat tggaagacca atcaagtcac
    38401 tatcgctaaa tttaacaccg acacgttcgt tacggtcatc tgtcaagact tcataaccag
    38461 ctcccatcaa gcttgcttca agtttttctg tcaaggcttg cgcttcttca tccttgacat
    38521 tgacagtaat caaatgcaca tcaaatggtg ccaattcttt agggaaattg attccccaag
    38581 cgtaacggta ttcacctttt ggcgttttgt taacaaagag gcgagcgtgt tgctccatca
    38641 ctgctgaaag aagacggctg acaccgatac cgtaacatcc catgatgatt ggcacagcac
    38701 gaccattttc atccaagaca tctgctccca tgcttgctga gtagcgagtt ccgagtttga
    38761 aaatatgacc gatctcaata ccacgcgcaa agttaaggac accttgtcca tctggggaaa
    38821 tttcaccctc acgaacttca cggatatcca catattctgc agtaaaatca cggcctgggt
    38881 tcacaccagt caagtggtag ccatcttcgt tagcaccgac aactgcattg cgaacatctt
    38941 gcaccttacg atctgcaata attttaatat tctctggcaa accaactggt ccaagtgaac
    39001 caaatcctgc ttgaacaaca ttcgccactt cttcttcgct agcaacgtca aagaaatctg
    39061 ctcccaagtg gtttttcaac ttgacttcgt tgagttggtc atttccaact agaagggctg
    39121 caacaagctc accatctgca atgtagaaga gggttttaat cgtttgttct tctggaacat
    39181 tgaggaaagc tgcaacttca tcaattgatt taacatctgg cgttgcaaca cgagtaactt
    39241 cttcttcagc gacaacacgg ttgcttggtt tgtactcgtt tgttgccatt tctaagttag
    39301 ctgcatagct agactcactt gagtaagcaa tggtatcttc accagagact atccatttga
    39361 gcaattctgc cttgatttct tcttgcactt ctgcaggaat ttcgtcaaat gaggcaactg
    39421 acttgtccaa gacaacccag cggtcaaggt ctgtacgagc agatgtaatg gccataaatt
    39481 cttggctatc cttaccaccc atggctccac cgtcaccaat aatagccttg aagtctaaac
    39541 cactacgagt gaaaatacgc tcataggctg ctttgtactc atcataaaca ctatccaaac
    39601 tatcatagtt agcgtggaaa ctataagcat ccttcatgat aaactcacgt gtacgaagaa
    39661 gtccattacg tgggcgtttt tcatcacgat acttgggctg aatttgataa aggttgagtg
    39721 gcaattgctt gtaagattta acagaatcac ggacaatagc tgtaaaggtt tcttcgtgag
    39781 ttggacctaa gataaagtct gatttttcac ggtttttcag tttgtaaagg tcttcaccat
    39841 aggtttcgta acgacctgat tcacgccaca attctgcact aagaagggct ggagccaaca
    39901 tctcaacagc accaatcttt tcgaattctt ggcgcatgat gtttttagct ttttcaatca
    39961 cacggttggc aagtggtaga taagaataaa cacctgctga aacttggcga acataaccag
    40021 cacgcaacat aagagcatgg ctgataactt gagcatcgct tggcatttcg cgaagcgttg
    40081 ggataggcat tttactttgt ttcataatat tcctcgatta tctaaaaaag agtcgcataa
    40141 tgtcattcca agtcacagca atcatcaaga caaccatgat gaccactccg gccaaggtga
    40201 cataggtttc aatttcttgt ttcaatggtt tgcggcggat ggcttctagg atattgagca
    40261 caatcttacc accatccaag gctggaatcg gaataagatt aaaaatccca atattgatgg
    40321 aaatcattgc caagaagtac aagatatttt caattccatt tttagcagca tcactacttg
    40381 ccttaaagat agcaacaggt ccacccaact tgttcaaatc cggttggaaa atcagatttt
    40441 tcagagctga gagaattcgg agagctgagt cagcagcagt tgtaaaacca cctacaaaca
    40501 tggatagaaa atctgactta acccccggtt gaacacctag aaggtaacga ccttgactat
    40561 cttcgggtgt aacagtgact tgtttgtcac tccccttttc agaaatagtc acatccaaag
    40621 tcggtgccgt cttatctttg gtttctgttt ccacagcttg gatcaagctt tcccagttgc
    40681 taacctcatg tgagccaatc ttggtaattt gtgccgtttc tggtactcct accttggcca
    40741 aggcaccttg gggcatgata tggaactgat tggtatcaac atctctgaca ccaccctgca
    40801 taaagattaa aacccaaaaa acaacgacac ctaagataaa attgttcata ggacctgcaa
    40861 aattggtaat cagtttgccc cagatagtcg cattttgata ttgaacatct aaaggtgcaa
    40921 tccgaacctc agtaccatct gcttccacaa ccgttgcatc gtgatccact gcaaatgttt
    40981 tttcttcttc cagaaccaat cctttgataa agagcttgtc ttcaaaatca aactgggtca
    41041 cctgcatagg gagggctgtt tgatccaatt ttttacctga gagattgatg cgtttaacct
    41101 taccatcatc agcaagtgtc aaactaacag gcgttcctgt cttgatttca gttgtatcat
    41161 caccccaacc ggccatgcgg acatagccac ccagaggcaa aattcgaatg gtataggccg
    41221 ttccatcctt accaatgtga gcaaaaattt taggtcccat accgatggca aattcacgta
    41281 ctaaaatccc tgatttcttg gcaaagtaga agtgcccgaa ctcgtgcacc actacaataa
    41341 tcccgaaaac cagaataaag gttaaaattc cgagcatagc gtttcctccg tcttttgatt
    41401 aaaagagtcc aaataagtgc atgattggaa atacaagcaa catactatcg aaacgatcca
    41461 aaacaccacc atgtccaggg ataaatttcc cagaatcctt aacaccaaaa tgacgtttga
    41521 tcgaactttc tagtaaatca ccaaattgtc cagcaatgct aaagaaaata gcaaagactg
    41581 acatcttgta aattccatat ggaagagcaa ctgtactgtc aactatcata aagataatgg
    41641 ttactaaaat tgctcctaaa ataccaccca aggcaccctc aagggtttta ttaggcgata
    41701 cccttggtgc taactttcgt ttcccatagt tcatcccaac aagataggca ccactgtctg
    41761 tcgcccagac gatacacaag gctaagagag ccttgtccaa acctgcaaca cgagcatcta
    41821 gtaaagcatt aaatccaaag cccacgtaga agctcatagc aagagggaaa accgcatcct
    41881 caatcgtata agacttgcta aaaacggtcg ttcctaacat gattgaaatc aaaacactat
    41941 aggcaaccac attcccatca actggcaaaa aagtcaggta attctccaag ggaatggtca
    42001 atgcaaaggt tgcaaagagg gtcaagaggc cctccatcgt catggtctct agacctctca
    42061 tcttcaaaag ttcatgcatg gctagcatgg ctatgattcc gattgctatc tgaagcaaga
    42121 ggcccccaat cattaaaatt ggtaggaaaa tagccagggc aatccctgca aacaaggttc
    42181 ttttctgtaa atcctgggtc atatttcctc ctaaactcct ccaaatcggc gatgacgacg
    42241 attataggca agaatagctt cctgcaaggc cgcttcgtca aaatcaggcc ataaggtgtc
    42301 cgtaaaataa agctcactat aggctccctg ccatggaagg aaattgctca aacgtaattc
    42361 tccactagta cggataatca agtctgggtc tcgtaagtcc ttaggcaaat gctgagtaaa
    42421 gagatagtta ccaatcaatt cctctgtgat gtcacctggg ttgattttgg catctaaaac
    42481 atcctgggaa atcaacttaa gcgcctgtgt aatctcagca cgtccaccat agttaagagc
    42541 aaaattaaga atcaatcctg tgttgttctt agtcaattcc tcagccttgg ttaaagcttc
    42601 aaaggtttgc ttaggcaggc ggtctgtctc cccaatcatt tgaatcttaa cattattcgc
    42661 atgtagttcc gggacataat tatcataaaa ctctactggc aagttcatga taaacttgac
    42721 ttcctgatct ggacgggtcc agttttccgt agaaaaagca tagaccgtaa taaccttgac
    42781 gcccagtttg ttggctgcct tggtcacggt ttgcaatgct tccatgcccg ccttatgtcc
    42841 aaaaactcgc ggttgcatac gttttttagc ccaacggcca ttgccatcca tgatgatgcc
    42901 gatatgagca ggaacctgtg tcggaacctc tacttccaca gccttatctt tcttaaaaaa
    42961 tccaaacatg atcttattcc tattcaaaaa tctatcgttt cattatacca tatttcccca
    43021 ttttcttcta tcactaagct atttattctc aggcaccatg cccatttttc aaaaaaataa
    43081 gccgcctgat tgggcgactt tatttttata gggagattat tatgaaaaag ttttaggagt
    43141 ttaagttaag gtcttcttaa cttatgaact tagtgtacac tccctagctt aaagtttcct
    43201 taagtatttt taaaaatcaa atttttccat ttctcctgcc aatttttctt ggataaacgt
    43261 gtttgataga gttccattcg gtcttcattt tctaagaaat gaggagttgg acgaacttga
    43321 aaattcaaaa tatcctccaa accataaggt gcatagagtt caaaatctga ttcttcattc
    43381 aagcgcagtc caactgccgt acaccgttct ggatacttac tcatagcatc acgagaactg
    43441 gtataggaag cagtgtgagg actgtgctga tgcatataga cctgattttt caattcccac
    43501 tggtactgag gaaaatcctc tctcagcttt ttctccagta ataaggtttc ctcataagaa
    43561 aaatctggat caaagaaaat cacatctata tctgtttcat gatcaaaagg ggatttgtct
    43621 gacaaaagat tccagatgaa atttctgaca gaacctgctg ccaactacga gtctttcaaa
    43681 ccaaggtctc ggatgatcgt cagaatggcc atcatatctg gattttctct aaaagcctct
    43741 aagatttctt gcttattttt cactgtattc ataacctaag tgctcatatg ccttagcagt
    43801 cgccacccgt ccagaccgtg tccgcatgat aaaacctttt tgaatcaagt aaggctcata
    43861 catgtcttca actgtctcac gctcttcggc gatattgaca gaaagagttc ctagaccaac
    43921 aggtcctcca ctgtacatct caatcatggt acgaaggatt ttttgatcca catagtccaa
    43981 accttcatgg tcaacatcca gcatagtcaa agccttatcg gtaataatat catcgattac
    44041 cccattcccc attatctggg caaaatcgcg cacgcgcttg aggagacgat tggcaatacg
    44101 aggggttcca cgactacgta gggccaactc agatgctgcc tcatgagtga tttccatctc
    44161 aaaaatatct gccgtccgct cgacaatttc tgtcaagtca gcatgggcat aatactccat
    44221 atgacctgta atcccaaaac gtgcccgtag tggatttgag agcataccag cccgagtcgt
    44281 cgcaccaatc aaggtaaaag gaggcaactc caaatgaaca ctgcgactgc cttcaccagc
    44341 cccaatcata atatcgatgt agaagtcctc catggcacta taaagcactt cttccactga
    44401 catgggtaag cgatgaatct cgtcaataaa gaggacatct ccaggctcta actcattcaa
    44461 aatcgctacc aaatcacccg ctttttcgat aacaggacca gacgtttgct tgagattgac
    44521 tcccagttca ttggcaatga caaaagccat ggttgttttc cccaaacctg gaggcccaaa
    44581 taagaggaca tgatccagcg cttcatctcg cattttggca gcttctataa agatttgaag
    44641 ctggtcctta accttatcct gcccaatata ttcacgtaaa tactgaggac ggagcgtgcg
    44701 ttctactaac tcctcatccc ccattatctc attatctaaa attctactca tggctctatt
    44761 atatcaaaaa accaagccac aaacaaaaaa gccacctgat tgggtgactc ctaagtttag
    44821 cacttatgtg gtataatatt ctacgacact gctacaccgc ctacgaaagg aggtgagata
    44881 gcccatgatg gaattagtac tcaaaactat catcggacca attgtggtcg gtgtcgttct
    44941 tcgtatagtc gataaatggc taaacaagga caaatagtgt caaaaaagac cccaagctta
    45001 tttggtcgtg agcttggggt cttttctagc ctatgatata gaactagtac tcaattcctt
    45061 tttattatcc catagttcac gaattttgtc aaaggtttac attttcttca accgctgtac
    45121 gacaagacgg ttaagattaa gagaacgtta gggattctat caatttcata gaaattttga
    45181 tttcgtaaac gaagagacaa tcttacatgt cacttctcat ttaatacgcc actactagac
    45241 aagcaaaatc attattacag tagttccagt tcttcaatta acagtcactt acaatcaaat
    45301 tgagtttgaa ctagctgaag cgaccacaga cctatttctt agttatattc gctaaaaaag
    45361 tccccgccaa ttccccgacc aaaatccgaa aaataccgaa aaatatcgaa aaattatttt
    45421 tagaatagtc cccaaaagcc tgaaatagag ctaaaaaact ccacctgatt gggtggagtt
    45481 aagg
//
LOCUS       DAVHIT010000004        43806 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVHIT010000004 DAVHIT010000000
VERSION     DAVHIT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.004"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(375..1601)
                     /locus_tag="VVN16_000145"
     CDS             complement(375..1601)
                     /locus_tag="VVN16_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU5571600.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     QYTQTPKTVCLNSATAALELILRVLEVGPGDEVVVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDLFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPAGSIADFTSFSFHAVKNFTTAEGGSATWKANPAID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYS
                     GLLQRRKDIVDRYDRGFAGSRIHPLAHKTETVESSRHLYITHVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMANYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTSSKK"
     gene            complement(1644..2336)
                     /locus_tag="VVN16_000146"
     CDS             complement(1644..2336)
                     /locus_tag="VVN16_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5571601.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVV
                     LVLTSPIFLIMSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     PAGITSPASIHYKDEDTIISQMTEKGLSVDQAYVEYVLPEKMRYNLAYLREFSFLGDI
                     KIMFQTVFEVLK"
     gene            complement(2520..4262)
                     /locus_tag="VVN16_000147"
     CDS             complement(2520..4262)
                     /locus_tag="VVN16_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571602.1"
                     /translation="MQNKKEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTMVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTTLVLIFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDSVDIRAYSQEELRTNIGLVLQDPFLYHGTIKSNIAMYQEISDEQVRAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMS"
     gene            complement(4252..5997)
                     /locus_tag="VVN16_000148"
     CDS             complement(4252..5997)
                     /locus_tag="VVN16_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571603.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYTIDSFAFENEETLTNVHFSLEKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGTI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLAQV
                     YQDIVAMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGTHDDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6203..6499)
                     /locus_tag="VVN16_000149"
     CDS             complement(6203..6499)
                     /locus_tag="VVN16_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU5571604.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(6652..7107)
                     /locus_tag="VVN16_000150"
                     /pseudo
     CDS             complement(6652..7107)
                     /locus_tag="VVN16_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7241..8068
                     /gene="xth"
                     /locus_tag="VVN16_000151"
     CDS             7241..8068
                     /gene="xth"
                     /locus_tag="VVN16_000151"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5571605.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8080..8331
                     /locus_tag="VVN16_000152"
     CDS             8080..8331
                     /locus_tag="VVN16_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU5571606.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLSL
                     EECQLMLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLY"
     gene            8338..8466
                     /locus_tag="VVN16_000153"
     CDS             8338..8466
                     /locus_tag="VVN16_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU5571607.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPNGYFLRFSE"
     regulatory      8849..8944
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9344..9925
                     /locus_tag="VVN16_000154"
     CDS             9344..9925
                     /locus_tag="VVN16_000154"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU5571608.1"
                     /translation="MKLLEDRILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            9925..11187
                     /locus_tag="VVN16_000155"
     CDS             9925..11187
                     /locus_tag="VVN16_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU5571609.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11238..11699)
                     /locus_tag="VVN16_000156"
     CDS             complement(11238..11699)
                     /locus_tag="VVN16_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU5571610.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(11696..12460)
                     /locus_tag="VVN16_000157"
     CDS             complement(11696..12460)
                     /locus_tag="VVN16_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU5571611.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVHRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(12641..13045)
                     /locus_tag="VVN16_000158"
     CDS             complement(12641..13045)
                     /locus_tag="VVN16_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5571612.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(13115..14596)
                     /locus_tag="VVN16_000159"
     CDS             complement(13115..14596)
                     /locus_tag="VVN16_000159"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU5571613.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(14615..15793)
                     /locus_tag="VVN16_000160"
     CDS             complement(14615..15793)
                     /locus_tag="VVN16_000160"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU5571614.1"
                     /translation="MAQHLITEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLERDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            15958..16965
                     /gene="galR"
                     /locus_tag="VVN16_000161"
     CDS             15958..16965
                     /gene="galR"
                     /locus_tag="VVN16_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU5571615.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAI
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(17011..18048)
                     /locus_tag="VVN16_000162"
     CDS             complement(17011..18048)
                     /locus_tag="VVN16_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5571616.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(18049..18402)
                     /locus_tag="VVN16_000163"
     CDS             complement(18049..18402)
                     /locus_tag="VVN16_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5571617.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(18646..19536)
                     /locus_tag="VVN16_000164"
     CDS             complement(18646..19536)
                     /locus_tag="VVN16_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5571618.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     VSLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            19669..20205
                     /locus_tag="VVN16_000165"
     CDS             19669..20205
                     /locus_tag="VVN16_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5571619.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIINLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(20372..21178)
                     /locus_tag="VVN16_000166"
     CDS             complement(20372..21178)
                     /locus_tag="VVN16_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU5571620.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(21523..23043)
                     /locus_tag="VVN16_000167"
     CDS             complement(21523..23043)
                     /locus_tag="VVN16_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU5571621.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALMAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(23036..23764)
                     /locus_tag="VVN16_000168"
     CDS             complement(23036..23764)
                     /locus_tag="VVN16_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571622.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(23779..24543)
                     /locus_tag="VVN16_000169"
     CDS             complement(23779..24543)
                     /locus_tag="VVN16_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5571623.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(24547..24990)
                     /locus_tag="VVN16_000170"
     CDS             complement(24547..24990)
                     /locus_tag="VVN16_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5571624.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(25672..25968)
                     /locus_tag="VVN16_000171"
     CDS             complement(25672..25968)
                     /locus_tag="VVN16_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU5571625.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(25990..27054)
                     /gene="pepA"
                     /locus_tag="VVN16_000172"
     CDS             complement(25990..27054)
                     /gene="pepA"
                     /locus_tag="VVN16_000172"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU5571626.1"
                     /translation="MTTLFSKIKEVTELAAVSGHETPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            27454..28155
                     /gene="galE"
                     /locus_tag="VVN16_000173"
     CDS             27454..28155
                     /gene="galE"
                     /locus_tag="VVN16_000173"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5571627.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLKENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPINPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            28577..29536
                     /locus_tag="VVN16_000174"
     CDS             28577..29536
                     /locus_tag="VVN16_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5571628.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGIVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            29526..30482
                     /locus_tag="VVN16_000175"
     CDS             29526..30482
                     /locus_tag="VVN16_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU5571629.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            30479..31231
                     /locus_tag="VVN16_000176"
     CDS             30479..31231
                     /locus_tag="VVN16_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571630.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            31327..32292
                     /locus_tag="VVN16_000177"
     CDS             31327..32292
                     /locus_tag="VVN16_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5571631.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPREVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKSLAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(32517..32759)
                     /gene="yidD"
                     /locus_tag="VVN16_000178"
     CDS             complement(32517..32759)
                     /gene="yidD"
                     /locus_tag="VVN16_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5571632.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(32759..33481)
                     /locus_tag="VVN16_000179"
     CDS             complement(32759..33481)
                     /locus_tag="VVN16_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU5571633.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLIIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(33468..34037)
                     /locus_tag="VVN16_000180"
     CDS             complement(33468..34037)
                     /locus_tag="VVN16_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU5571634.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(34056..34784)
                     /locus_tag="VVN16_000181"
     CDS             complement(34056..34784)
                     /locus_tag="VVN16_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU5571635.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLESKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(34784..35524)
                     /gene="xerD"
                     /locus_tag="VVN16_000182"
     CDS             complement(34784..35524)
                     /gene="xerD"
                     /locus_tag="VVN16_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU5571636.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISSCNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(35515..35976)
                     /locus_tag="VVN16_000183"
     CDS             complement(35515..35976)
                     /locus_tag="VVN16_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5571637.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDSEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(35973..36494)
                     /locus_tag="VVN16_000184"
     CDS             complement(35973..36494)
                     /locus_tag="VVN16_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5571638.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(36470..37441)
                     /locus_tag="VVN16_000185"
     CDS             complement(36470..37441)
                     /locus_tag="VVN16_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU5571639.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(37438..38232)
                     /gene="racE"
                     /locus_tag="VVN16_000186"
     CDS             complement(37438..38232)
                     /gene="racE"
                     /locus_tag="VVN16_000186"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5571640.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(38439..38687)
                     /locus_tag="VVN16_000187"
     CDS             complement(38439..38687)
                     /locus_tag="VVN16_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU5571641.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(38943..40568)
                     /gene="treC"
                     /locus_tag="VVN16_000188"
     CDS             complement(38943..40568)
                     /gene="treC"
                     /locus_tag="VVN16_000188"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU5571642.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(40746..42713)
                     /gene="treP"
                     /locus_tag="VVN16_000189"
     CDS             complement(40746..42713)
                     /gene="treP"
                     /locus_tag="VVN16_000189"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU5571643.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEIISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            42898..43608
                     /gene="treR"
                     /locus_tag="VVN16_000190"
     CDS             42898..43608
                     /gene="treR"
                     /locus_tag="VVN16_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU5571644.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
BASE COUNT        12796 a        10097 c         8371 g        12542 t
ORIGIN      
        1 aaaactttga acaatattct gaaaacaaaa aagaaagacg cgagtttcca caacctcatc
       61 gtggaaactc gcgtctttca aaaaataggg tgaattgaaa actagaacaa gacactacat
      121 tttctaaatg atgcctaata aaaattctaa atgctacacc aaattgctca catctcattt
      181 caaaccatac agtatattga aactataaga gtacaccttt gcttctaaaa tattgctaga
      241 aattaatttg actatcctga ttgatttgtc ctgtttttat ttcattttac tataggactt
      301 tcaattgaaa acaacagttt tacactcaat tagacttttt agaaacagaa tcactattac
      361 ttgactgtag tttgtcattt ttttgatgaa gttagcactt tttcagaaac tgttttgaaa
      421 gtctcaatga tatagtccac ttcttcatca cttaatttag tgtgaagagg aagggtaatt
      481 tcattttcaa agaaggcata ggccttagga tagttcgcca tgtcaaaacc aagattctta
      541 taggctgtca agagcggaag cggtttgtag tgtacgttac ttgcaattcc tgccttggcc
      601 aattcttgga tgataagatt gcgttcttct aagcttgctc cttctacatg ggtaatgtag
      661 aggtgacgtg aagattcgac agtttcagtc ttgtgtgcta aaggatggat gcgagaacct
      721 gcaaaaccac gatcatagcg gtccacaatg tccttacgac gttgcaacaa accagagtaa
      781 cggtccaatt gcaccaaacc aagcgaagcc atgatatcgg tcatattgca cttgtaggct
      841 ggtgtgacga tatcgtattc ccatgaaccc agttgcatct tagcaagagc atccttagtt
      901 tgcccgtgaa gggaaagaat ttggaattct ttgtacatct cttcgtcgtc aatcgctgga
      961 ttggctttcc aagtcgcact tccaccttct gctgttgtaa agttcttaac ggcatggaat
     1021 gagaaggaag taaagtcagc gatagaacca gctggttgtc ctttataagt agatcccaaa
     1081 gcatgggcac tatcagaaac aatcacgata cggttaaagg ctttttgcca cttgcttgaa
     1141 gcagtaaaga ggtcacgttt cttctccact acttggaaca aacggtcata gtcgcaaaca
     1201 atccctgcaa gttctactgg aatgattacc ttggtctttt cagtgatagc ttgctccagc
     1261 aagtcataat ccatctcaaa cgtatctgct tggatatcca ccatgacagg tgttgctcct
     1321 acgtgagtga tgacactaca tgaagctgta taggtcatag ctggaacgac gacttcatca
     1381 ccaggcccca cttccaagac gcgcaaaatc aactcaagag cggcagtcgc agagtttagg
     1441 cagacagtct tgggtgtctg tgtgtattga gacaagcgac gctccaattc ttttgtctta
     1501 ggaccggttg tgatccaacc agaacgaagg gtatccgcta cttcagcaat ttctgcttct
     1561 gtaatatcgg gtggtgaaaa tggaatattg taatttggca ttgatttgct ccttttatct
     1621 gtactcattt tctcatgact actttatttt agcacttcaa acacggtttg aaacatgatt
     1681 ttgatgtctc caaggaaact aaactctcgg agataggcta ggttatagcg catcttttca
     1741 ggtagaacgt attcgacata ggcttggtca accgacagac ctttctccgt catttgactg
     1801 atgatagtgt cctcatcctt gtagtgaatg ctggctggag aggtaatccc tgctggcaag
     1861 agcaaggtcg ccatcatttc agggctatac tgctctgtgt aacgaggcac ttcaggacgc
     1921 gtccctacaa aagacatctc gcctttaagg acattgacca actgaggcag ttcgtccaaa
     1981 cgcacacggc ggatgaaatt accaaccttg gtaatgcggc tatcgttagc agaagtcact
     2041 agacttcctt ttttatccgc atccgtcacc atggtacgga acttccaaat cttgaaccga
     2101 cggttgtact gggtcacgcg ctcttgcttg taaatcactg gccccttgct atccaacttg
     2161 atccaaatgc tcatgatgag aaagatggga gaggtcagaa ccagtacaac caaggccaaa
     2221 acccagtcca aacaacgctt gaaaatcagc gaacccttcc ttttagagac aagctggtag
     2281 taagactcaa cctcgcttga tttcatttcc acgggcaagt cttcccattt cagcatgctg
     2341 tttctccttt gtttttatta tactatttgt caacattttt cattatacca caaaatggaa
     2401 aaggcggtga aagaaatctg taatttgaag gatttccttc ttacactcaa tgaaaatcaa
     2461 agtacagctt ttaggttgca gataaagctg acatggcttg aatagatttt caaagagtat
     2521 taagacatgg ctcctgcctg caagctatac atcttgtgat aggttcctcc caaggccaaa
     2581 agttcctcat gggttccgct ctcgataatg cgccccttgt ccaaaacata gatacagttg
     2641 gcgtcttgga tggtagagag acgatgggcg atagcaatgg ttgtccgtcc ctgtctcatc
     2701 ttagccagag aagcctgaac cagactttct gtttcagagt caatattggc tgtcgcttca
     2761 tccaaaatca ggattttagg ctggctggcg actgttctgg caaaggcaag aagttgacgc
     2821 tgacctgttg aaaaactgga gccacgctcg gaaacagggg aatcataacc ctgaggaagt
     2881 tcttgaataa aggaatctgc atccacgaag gctgctgcag cccggacctg ctcatcactg
     2941 atttcttggt acatggcgat attggactta atagttccgt gatagaggaa gggatcctgc
     3001 aagaccagac cgatatttgt tctcagctct tcctgactgt aggccctaat atccacacta
     3061 tccaagagaa ctcgccctga ctgaaattca taaaagcgca tgaggacatt gataatcgaa
     3121 gatttccctg aacctgtatg acccacaaag gcaatggttt cacccttatt aacggagaag
     3181 gaaatgtcat ccagaatcgg atgttttcca tcataagaga aacatacatg ttcaaaacgg
     3241 atattgcctt ctttgacttt agcctgccca ttttcttgaa ggggctcata ggtcctctca
     3301 tcgattaagg caaagacacg gcctgcagaa accatagacg tttgcagtgt tgaaaagttt
     3361 tgcgtcacct caatcaaggg gtcaaagagg cggttgatgt actggataaa agcatacatg
     3421 gtcccggccg ttatcccgat agaaagacca cgatagccaa aataggccat caagactgca
     3481 tagcctagaa gtttcagcaa actcatagca gggcgcaaaa agagggcatc caaggctaca
     3541 gaacggttgg catagaccaa gtgctcttgg ttgatttcat caaattctgc ctgcaggcgc
     3601 ttctcctgat taaaggcctg gattattctg attccctcga tgttttcagc cagcttacta
     3661 ttgatatctg ataagagact tctggttttt tcgataatct tgacggattt tttccgatag
     3721 agattgacca aaaggaaaat caagggaaga aagatcaaga ctaaagtcgt caaacgaaaa
     3781 tccaacacca acatggtata aagggttgtc agaaagatga aaactgctga gataaagctg
     3841 gacaaaatcc ccgaaaacat atcactgatg gtctcggtat catttgtcaa acgagagacg
     3901 atagagcctg ctggcgtctt gtcaaaatag gacatgccca atttctccat attagcaaag
     3961 gcatcccgac gaatatccct aacaatactg taggacaccc gtgcaaagag aagattaccg
     4021 acatactgta ccatagtttg taggatataa aggccatagt agaccagcaa aacggtcaca
     4081 gcgagttggt tgagattact cagatactgg tcaataaagt gggaagccac aaggggaatg
     4141 acacttttga tgaccgtcgt cgctaggaga aaactgagtg ccaaaaaagt caggagtcca
     4201 taaggcttga gataagacat caagcgcttc aatacagccc attgttcttt cttattctgc
     4261 atcttcttct cccttcattt ccagttgctg agactggtag gtttgggcat accagccatc
     4321 caaagctagc aagtcatcgt gtgtacctcg ttcgatgatt tgaccatttt gcaggactaa
     4381 aatcaaatct gcatgtacaa ctgcactgag gcgatgagcc gtgataatgg ttgtcttgtc
     4441 cttacgtgtt tccttaagat tgtcgataat cgcatactct gtcttggcat ccacggccga
     4501 caaagaatca tccaaaatca agatatcagg gtctaaaatc atagcccgac tcatggccag
     4561 acgctgcttt tgacctccag aaagactgac tcctttttct ccaatcagcg tatcaaatcc
     4621 ctgaggcatg gctacaatat cttggtaaac ttgggctagc ttagttgctt cctcgactgc
     4681 tgaaaggggc aagttaggat tgccaaagcg aatattgtct aaaatcgaag tcgcaaagag
     4741 gaactgatcc tgaggaacat agcccataag actacgaaga tctgtcagac gatagtcacg
     4801 aatatcgtga ccatttagat aaatggttcc cttgtccaca tcgtattcac gcaagaggag
     4861 cttgatcaaa gatgttttcc cagagcctgt ttgcccaacc aagcccaggg tttgaccttt
     4921 ttccaaacta aagtgaacat ttgtcagtgt ttcctcattt tcaaaggcaa agctgtcaat
     4981 ggtatactcc aaacgcccat tttcaatacc atccagagga aactcagggt cttgtacagg
     5041 agattcctga gacaaaagat tttcaatccg ctggtaagaa accttccctc gctgagtagt
     5101 attaaagagg aagccgatgg ccataagagg ccagaccagc atatccaaat agctgataaa
     5161 ggtgactaga ttcccaactg taatctgccc ttcctgaacc atcaaggagc caaccaaaag
     5221 cgttaaaaca taggacgaac caacaaacac gagaaccata gggtcaaaga gactatcata
     5281 tttcatggtt tgcaggttct tttggaaggt taattcattg actgcctgaa aagacttcaa
     5341 ctcgtctgcc tgataaccga aagacttggt cactttgata cctgatacgg actcctgtac
     5401 cttgttattg agttcagaaa aagcagcttg ggattcgcca aaggccttat gagtctttct
     5461 ccctaggcga ctagtcgcat aggccatgaa aggtaggggg agaatggcaa caagagtcat
     5521 ctgccatgag atgctaaaga gcatggtcaa caaagtcacc agagccgtga tagaggcatc
     5581 caccgcagac atgacaccgc cacctgctaa acgagtcaag gcattgatat cattggttgc
     5641 gtgtgccatc agatcacccg tccgataggt ttgataaaag gctgatgaca tttttgtgaa
     5701 atgcttaaac aagcgagacc gcatgatctg tcccaagcaa taagaggtcc caaggatata
     5761 catacgccac acatagcgca aatagtacat accaaaggct gcaagtagca agtaaaatag
     5821 gctaagaagg aggtcctgct gggttaattg ccccgatgtg atggcatcaa tgacccgccc
     5881 cataaccata ggaggaatga gattgaggac ggaaaccaag accaaggcca caatcccgac
     5941 tagataacgg cgtttttcta acttgaaaaa ccaccagagt ttttgaataa tggacataaa
     6001 atccctttct gatcgcaaat ggaaacctga ggccaatact caatgaaaat caaagagcaa
     6061 actaggaaac tagccgcagg ctgctcaaag cactgctttg aggttgtaga tagaactgac
     6121 gaagtcagta acctacatac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag
     6181 agtacaagac cccaggtttt tcttattcat aagttacgac tgtaacagca cccttgtcat
     6241 attcagcaat aaagatattg gctacattgt catgcccttg tttgctgagg ttatcaagca
     6301 accactcttc gctacgacca atcgattcca agacatctac ttgaatcaca ccgtcagtca
     6361 caactggata cttaggattt tcatctccca tttgcacaac gatgagttgc ccattttgct
     6421 cttgcacagc tcgtttgact tgcttcatct ggaaaatccc ttggctacga agtttgaggg
     6481 ctacatccga tgcagacaaa ccaactgaac gacaggctta tgggtcaata ttcccatttt
     6541 tgataaggag agttggctta ccatcaatca aacgtttcac aaagtgaaca ttgttgttga
     6601 gctatttgag agtcaagacc agtttgatta aaatttcgat ataattaagt gtcatttgtt
     6661 cacatccacc agttcaattg cgtctgtctt atacaattcg actttctcta acaggtactt
     6721 gtctggctca cttccgttta gggcacgata atagcgatct cccaccttga taagtgcgcc
     6781 atctgtgttt gtggaagtat taacatagac ttctgacttg tctactccca aatctttgga
     6841 aacaacctcg atgaaatgaa gtgaagtttg aaatttattg ttagaaactt gattgttttg
     6901 ataagtcgtg atgctgacca aaagcagagc taaataaggc cagagctgaa atcatgacca
     6961 actcacgaaa ttcggttccc tttttatcat ggtatgcctt aaaagcaaaa aaactgtcac
     7021 agctaataga acaatcccaa agccaaccat gataccattt tgctgaccga tttggctgag
     7081 tacatagtca taagagtaga atttcatgta ttttcactcc ctttcataaa atcattctta
     7141 attataaacg aaagagagta atttttcaaa ctatacgttg gggaaataga tctttttttg
     7201 gtataataaa atctataatc tgaatgaaaa aggtaacttt atgaaactta tctcatggaa
     7261 tattgattcc ctaaacgctg ccctaactag tgactcagct cgtgccaaat tgtcccaaga
     7321 agtcctacaa accttggtcg ctgaaaatgc tgatatcatt gctatccaag aaaccaagct
     7381 ttctgccaaa ggacctacaa agaaacacgt ggaaatttta gaagaactct tcccaggcta
     7441 cgaaaacacg tggcgctctt cccaagagcc tgcccgtaaa ggctatgctg gaaccatgtt
     7501 cctttataag aaagaactta cacccactgt cactttccca gaaatcggtg ccccttctac
     7561 catggacttg gaaggccgca tcatcactct agaatttgat gcatttttcg tgacccaagt
     7621 ttacactcca aatgctggcg atggtcttaa acgcttggaa gaacgccaag tctgggatgt
     7681 caaatatgcg gagtatttgg ctgaactaga caaagaaaaa ccagtccttg cgaccggtga
     7741 ctacaacgta gcccacaatg aaatcgacct tgcaaatcct gctagcaacc gccgttcacc
     7801 tggatttact gacgaggaac gtgctggatt taccaacctt ttggcaactg gatttacaga
     7861 caccttccgc catgttcatg gcgatgttcc tgaacgctac acttggtggg cacaacgcag
     7921 caaaacttct aaaatcaaca atacaggctg gagaatcgac tactggctca caagtaaccg
     7981 tatcgctgac aaggtcacta aatctgatat gattgactca ggtgctcgcc aagaccatac
     8041 accgattgtc ttggaaattg acttgtaaga attttctata tggactacaa tgcggtcatt
     8101 cccgagttgc ttgtatcgaa tatcgaacag tcccggtcct tctactgtgg tttactgggt
     8161 tttaggattg aataccaacg tccagaagaa aactttctct ttctttctct tgaagagtgt
     8221 caactaatgt tagaggaggg aacaaaggat caactagctg aactgaccta tccatttggt
     8281 cgtggagtca atctctcctt tggcataaag gatgtttcaa aactctatta aagagtgatg
     8341 aaggcaaact atcctattta tcgccccttg accaaaagaa agtttcgtgt cagtgatccc
     8401 tatatctatc ctcataaatt tgcagttttg gatccaaatg gctatttttt aagatttagc
     8461 gagtagaata ataaaaattg ggaaaagtct ttaaagttag aaaaaggcat attgtcaggt
     8521 gtttaaaaac ttgatagtat gccttttatg atggaaaaat cttctaatac tcttcgaaaa
     8581 tctcttcaag ccacgtcaac gtcgccttac cgtaggtata tgatactgac tctgtcaatt
     8641 ctatccacaa cctcaaaaca gtgttttgag gttgtggata gcttcctagt ttgctctttg
     8701 attttcattt agtataaata ttagcttaca atggagtttt tgttcaccct ctcttctgtt
     8761 tgttcgtttt ttaaagcaaa acactaacaa aatagttgat ttataaagtt atttagtata
     8821 aaataaaaac attggctcaa gcctatttaa aattgaatat cgttttactt gtttatgtcg
     8881 tgaattggca cgacgtttct acaaggtgcc ggaacaccta acaataagta agtcagcagt
     8941 gagatatggc cgtttttgcc atgcctattt gggggactta cttagagatt aagtaggtct
     9001 tttttgaata tttttaatat ttcccttaat tagtgcggac gggaggtgaa attatcctgt
     9061 gaataatttc aggaatccag ggaatttcat ccctaatttt tgagccccta gctctttcat
     9121 ttctaggctt aggctaaaat agttccccga actattttac tctcaaaaat cccgtcatag
     9181 acaataacaa gatcattgtc tatgatacca ctacaacggg aaatatttga taagactatt
     9241 acgagcgaag cgagtcttat caaatatata gtattagcaa aagggatcac tatagaaaca
     9301 tttcaacaac aagtaaatta gcttttagac tttaggaggt cttatgaaat tattagaaga
     9361 tcgcatcctc aaggatgggc atatcttggg tgataacatc ctcaaggtag attccttttt
     9421 aacccaccaa gttgacttta gcttgatgcg agagattggt aaggtttttg cggaaaaatt
     9481 tgctgctgcc ggcattacca aggtcgtaac cattgaagcg tcgggtattg ccccagccgt
     9541 ttttacagct gaagccttaa acgttcccat gattttcgcc aaaaaagcta agaacatcac
     9601 catgaacgaa ggcatcttaa ctgctcaagt ctactccttt accaagcagg tgaccagcac
     9661 cgtttctatc gctggaaaat tcctctcacc agaggacaag gttttgatta tcgacgattt
     9721 ccttgctaat ggccaagctg ctaaaggctt gattcaaatc atcgaacagg ccggtgccac
     9781 agtccaagct atcggtatcg tgattgagaa atccttccaa gatggtcgtg atttgcttga
     9841 aaaagcaggc taccctgtcc tatcacttgc tcgcttggat cgttttgaaa atggtcaggt
     9901 cgtatttaag gaggcagatc tctaatgcaa actcaagaaa aacactcgca agcagccgtt
     9961 cttggcttgc agcacttact agccatgtac tcaggatcta tcctggttcc catcatgatt
    10021 gcgacagccc ttggctattc agctgagcag ttgacctacc tgatttctac agatatcttc
    10081 atgtgtgggg tggcaacctt cctccaactc caactcaaca aatactttgg gattggactc
    10141 ccagtcgttc ttggagttgc attccagtcg gtcgctccct tgattatgat tgggcaaagc
    10201 catggtagtg gcgctatgtt tggtgccctt atcgcatctg ggatttacgt ggttcttgtt
    10261 tcaggcatct tctcaaaagt agccaatctc ttcccatcta tcgtaacagg atctgttatt
    10321 accacgattg gtttaacctt gatccctgtc gctattggaa atatgggaaa taacgttcca
    10381 gagccaactg gtcaaagtct cttgcttgca gctattactg ttctgattat cctcttgatc
    10441 aacatcttta ccaaaggatt tatcaagtct atctctattt tgattggtct ggttgttgga
    10501 actgccattg ctgctactat gggcttggtg gacttctctc ctgttgcggt agctccactt
    10561 gtccatgtcc caattccact ctactttggg atgccaacct ttgaaatctc atctattgtc
    10621 atgatgtgta tcatcgcaac ggtgtctatg gttgagtcaa ctggtgttta tctagccttg
    10681 tctgatatca caaaagatcc aatcgacagc acgcgccttc gcaacggtta ccgcgcagaa
    10741 ggtttggccg tacttctcgg aggaatcttt aacaccttcc cttacaccgg attttcacaa
    10801 aacgttggtt tggttaaatt gtcaggcatc aaaaaacgcc tgccaatcta ctacgcagct
    10861 ggtttcctgg ttctccttgg actgcttcct aagtttggtg cccttgccca aatcattcca
    10921 agctccgtcc tcggcggtgc catgctggtg atgtttggtt ttgtatctat tcaagggatg
    10981 caaatcctag cccgagttga ctttgctaac aatgaacaca acttccttat cgcagctgtt
    11041 tcaatcgctg caggtgtcgg tctcaacaac agtaatctct ttgtcagcat gccgacagcc
    11101 ttccaaatgt tcttctcaaa cggaatcgtc gtagccagcc tactcgccat tgtcctcaat
    11161 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
    11221 aaaataaaaa catctaccta ttcatatatg ctagtgtcat agtcctgaaa atcatctgca
    11281 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
    11341 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatacttt
    11401 ttcagtttta gattgctttc caataattca cctattaaca ataatctatg ataggagcta
    11461 tgtagccact ctggcaattt atctcctact tcccacccat atttttcctt catcaaagct
    11521 agttgttttt cgtcaccaaa tttcatattt tcagataacc atctcacaca atcgggatct
    11581 gataaggtac agacgattaa acttaggtta tcgtaacggc tttcagtttc ataatcaggc
    11641 cagatgactt ttaagaaatc atacacctct tctgatgcaa caagttgttt cgttttcata
    11701 atttccgata ctttcctcta cctttaaatt ctattatttc tttgtctctt aatatttgaa
    11761 gctgttgcct aatcttttct ttgatatgat tgttcttaac aaagatattt tttaggtcac
    11821 tttcaaaacg atacatatct tcaagggtaa attctgaacc ctctatctta tctatacaat
    11881 ttagaatttc tattgtccaa cctcttgatg acagagagct cttccttaaa aataaacctt
    11941 gcttaaattc ttttgtaact ttttctggat ctctaacttg tccatcttgc acaagaaata
    12001 tccttccttt agaaggtact tgtgataaat caatgttaca accaatccaa cctgctcgtc
    12061 tagcagttgg tgcaagtggt tttctttgaa taatcgattt cggtgtaaca aattgcttcg
    12121 gaaggacaag aaagttattt acctcaaaat tttttgtgta agttaaaaaa aagaaattag
    12181 gattattatc tgcctgcaca cgcttcatca tcgttgcata agcaccatca ttgattgttg
    12241 atgaaaaatt tccttttttg ctctttagtt caaactcctc actacaatga ttacagtaaa
    12301 aatctgctac aggccgatta ttttcaaaat gatttaaggg attgttccca caattagggc
    12361 aataagactg acgatgaacc caatcctccg ttaagatacg tgccttttgg ctattacttt
    12421 tataggtttc tactaattct aaattaaagt gtaattccat ttaaaaattc ctcactaata
    12481 tgagaactca ctcgatataa gcaattaaaa tctactataa ctagtatacc acatatatgt
    12541 acctattaca tttgtatttc atcactctct tttatatttc cttctaacat atctcataaa
    12601 ttaaaaatga gtgagataaa tttatcccac tcacctttct ttatatactt acccgtcttt
    12661 cctcagacct ttggcctgtg acaaacatgg taaaggttgc tttacagaca tttctgccct
    12721 cttgattggt gatatccaca tccatcacac aggttgtacg accttgatgg acacattctc
    12781 ctttaatggt caatacatcg tcgagttttc ctgccttaag gtagttgata gaggattgga
    12841 gtgtcacccc atcaagtccc agcgagataa ccaccaaacc actgatttgg tcacatagtg
    12901 tgaatagata gccaccatgg gcattgccat agtagttgag cgacgagttc actactttgg
    12961 tcgtcaccac aacgtgacca tctctcattt tttcaatttc gtaattttca aaggcagata
    13021 tagcgtcaaa atgaaagtct ttcatcgttt cctcctgata tttcaaacga cactatgata
    13081 ctacttttta taggactgtg caaggagaaa gctcctagtc tagtaaaatt ccgacctgtt
    13141 ccacaaagcg cataaaggct gtctgccctt gttctgtctg cttgtagact cctgcatcct
    13201 caagtacacg cacaaagata gcacccacag agtccttgac gattgcaagg gctttttctt
    13261 tatccgttag gtctggatgt tgggatttga gttggtctgc ccactcctga tgataatcgg
    13321 caactgtaac agcttctcct acaagatagc tagcgacttg ctccacttct tctttcagac
    13381 gtggtggcaa gattgccaag cccatgacct caatcaagcc gatattttcc ttcttgatat
    13441 gttggacatc cttgtgggga tgatagatgc catcaggatg ctctgctgaa gtctgattgt
    13501 ctcgcaagac caagtccaac tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg
    13561 tgtgatgcgg tgtcccgtct gtctctgcta aaatctgcac actagaatct gaatactgac
    13621 gccattcctg gaaaatctta tcagctaagt taatcaaatc ctctttggaa tccgaagtca
    13681 agcgtaggac agacattggc cacttgataa ttccagcctt gacctgctca aaaccagcaa
    13741 atcggaaggt cttttgcaag ggagccaatt ccataggaaa tacgtgacgg cctccctgat
    13801 aatgatcatg agttagaata gagcccccca caatcggcag gtcggcatta gatccagcaa
    13861 aatatcctgg aaactgctct acgatagcca acagacgttc aaaactctga cgactaatgg
    13921 ccatgggacg atgctggcca tctaaaaaga tacaatgctc attaaagtaa gcatagggcg
    13981 aatactggaa cccccattcc tgaccaacca tttcaaaacg gataatccgg tggttgctgc
    14041 gagctgggtg gttgacccga ccatggtaac cctcattctc taaacaaagt tgacactgag
    14101 gataattact agcttgcacc aacttggcta ccgcaatctc tttcggatcc ttttcaggct
    14161 tagagagatt gatggtaatt tcaagttctc cataatcaga tggaacacga taagcgatat
    14221 ttttagcaat ggccttgagt ttgatgtagt catttttctg actgagttga taaaaatcct
    14281 ctatcgcttg ctcaggagag tggacatagg cttcccagaa gtcacgattg acctgactcg
    14341 gataaggagt caccaagtcc atcagttcag taccaaggat ttcacgcgca gtctgactat
    14401 cctcaatcat ctctaatcga acggcttcct caactagctg gtccttgagg tcaatcaatt
    14461 tatccagatt ggtctcaact tccaaaacac cttctcccac tcgtgccaaa acacggttgg
    14521 tcaggtagat tcgatccatt tcctcaaatg aactttcaga aattacatgt gttacaaatt
    14581 tatctactaa ggtcactata gagcctcctt ttgactagtc aaggacgcga gtgccacctg
    14641 caacttcagc gatatagaag cttggagcgt atccaactac ttcctcgtag tgtttgccta
    14701 cagcttcctt aaaggcctca acagtatctt tttgaaccaa ggcaatggca cagccaccaa
    14761 aaccagcccc tgtcatacga gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa
    14821 gagtatccaa ttccaaacca gttacttcat aatcacgctc cagagaaacg tgtgacgcat
    14881 tcatcaaacg accaaatgtt tccaaatcac ctgcttgaag ggctgcttga gctttaaggg
    14941 tacgttggtt ttcaagcaca gcatggcgag cacgtttcaa acgattttca tctttaatca
    15001 gatagctata ttggtcaacg gcccactcgt ccaattcacc cagggtctga atatccaagg
    15061 caacttgcaa ttcttccact gctttttcac actcagcacg acgttcattg tatttagagt
    15121 ccgccaattc acggcgtttg ttggtgttca tgataacaac gacattgtcc ttcaaatcaa
    15181 gtggcaccaa gtcgtattct aaagtattgg tatctaagta aatagcacgt tggtcagccc
    15241 ccataccaat agcaaattgg tccataatgc cagaatttac tccgataaag ttattttctg
    15301 tttgttttcc gattttaacc aaatcgagac gctctagttt taaatcaaag agatgctctg
    15361 ccacgactcc tgtcaagagc tccaaggatg ctgaagaaga caaaccagca ccatttggga
    15421 tattcccata aacataaaaa tcaaaacctt tgtcaatcac gtgcccagct tcttgcaaga
    15481 aatgaaggac accttttgga taattggtcc agttgtgctc tttttcaaac ttgaggtcag
    15541 cgagaggcac ttcgataatg cccttgtcct caaagttagc tgagtagaaa cgcaagactt
    15601 ggtcgtcacg cttacgagct gcaccgtaag ttcccaagga aatagcagca ggaaaaacgt
    15661 gcccaccgtt gtagtctgtg tgttcaccaa tcaaattaat gcggcctggt gaaaagaagg
    15721 tttggtctgc ttcttgacca aaaacagcaa gaaagtcttt gcgaaggact tcagtaataa
    15781 gatgttgtgc catatgaatt ctcctttgac tgtccgttaa gccatcttaa cgtgtaatcg
    15841 ttttctttct attgtatcca aggaatttcc caaggtcaat cctttttact aaaattttag
    15901 taaaatatca gtaaaaagag gaaaaacatg atatactaac ctaatcaagg aggatttatg
    15961 gctaccttaa aagacattgc acagctagcc tctgtctcta tcgcgaccgt atcccgcgtc
    16021 ctcaatcgcg accagagcct atctgttaca gaagaaacca gacaccgtat tttaaccgtt
    16081 gctgaagagc tgggctacac caagcacctc aagacaggcg agtcccacaa acccaagcaa
    16141 aagattgcca ttatccaatg ggtcagcgaa caaggggagc tggacgacct ctactactac
    16201 cagattcgcc taggaataga aaaaagagcc caagagttgg actatgatat cttgcgctat
    16261 tttaatgacc atccttttac cttaagcgag gaagtgattg ggattctctg catcggaaaa
    16321 tttagccgag ctcagatttc tgcctttgaa gaataccaaa aacccctggt ctttctagac
    16381 agtgatacac tttccctggg acatacctgc attattacgg acttttacac tgccataaaa
    16441 caggttgtcg attatttcct cagccaagga atggaccgta tcgggattct aacagggctt
    16501 gaggaaacaa cagaccaaga agaaatcatt gaggataagc ggctggaaaa tttcagaaac
    16561 tacagtcaag caaaaggaat ctatcatgat gaactgatct ttcaaggaaa ctttactgcc
    16621 cagtctggct atgacttgat gaaggaagcc attcagaact tgggagacca actcccacca
    16681 gcatttttcg cagccagcga tagtttagcc atcggtgccc ttcgtgcgct ccaagaagct
    16741 ggaatcagcc taccagaccg tgtcagcctc atttccttta acgacaccag cctgaccaag
    16801 caggtttatc ctcccctctc cagcatcaca gtctatactg aagaaatggg gcgagcaggt
    16861 atggatattc ttaacaagga agtcctccac ggtcggaaaa tccctagcct gaccatgctg
    16921 ggaaccagac tgacattaag agaaagtacc ctaaatcaag aataggataa cataaaaaac
    16981 gaatagagtt ctaaaactcc tattcgtttt ttattcgatt acaatcatag acttaatggt
    17041 cttacgttca tccatatctt tataggcctg gtcgatatct tccagtttat aacttgaagt
    17101 aaagacgcga cctggattga tatcaccatc aaggacggct tttagtaaaa attgcttatc
    17161 gtatgttgta gcagaagctg ccccacctgc tacagagata ttttgcataa atgtcgaacc
    17221 aagagcacga ttattatagt gtgggactcc tacaaagccc atacgccctc cattatgaag
    17281 aacacctagc gcctgttcta tagcagcctc cgtaccaaca cattcaagtg ctgcatctgc
    17341 tcctccaccg aggatttcac gcaccttggt aattccttct tgaccacgtt ctgcaacaac
    17401 agctgtcgca cctgactcca tagccatctt ttgacggtct tcatgacggc tcataaggat
    17461 aatttgtgat gctccacgca tcttagccgc gatgacagca cattgaccaa cagccccatc
    17521 accgataaca acaaccttgt cccctttttg aacatttgca acacgcgccg catgatagcc
    17581 tgtcggcatg acatctgcaa gagtcaaaag ggacttgagc atcccttctg tatagtcaga
    17641 aggttgacca gggattttaa ccagcgccca gtttgcatag tggaagcgaa tatattctgc
    17701 ctgaaaatca ccccccaaat tattgccaat atgattgtcg caagaaccgt caaatccagc
    17761 aagacaggca tcacactcac cacatccatg tgtaaaaggg acaatcacaa aatcacctgg
    17821 tttcaccgtc gtaatggctt ccccagcttc ttcaacaatc ccaatcgctt cgtgtccact
    17881 atttttgtgt ccagctttcg tttctggatt acggtacctc cataaatctg aaccacaaac
    17941 gcacgcacga accacacgaa taatcacatc atccgcttct attatttgcg gacgttcaat
    18001 actagcaagt ccaacctgac ctgcctttgt atatactgct gatttcattt aaaattttcc
    18061 ttccttataa agtttaattt tgagatttaa acgatttaaa gctgtctgta gctgagacaa
    18121 gcgctcctct aatttttgct tttcctcttc taaaatacca agcctctcct ctctcgtttc
    18181 atctcccttt tggtagagcg acatatagtc aactaaacta tctacagaga cacccgccga
    18241 acgaaaacac ttaataaatt ccagcgcttc gatatcctga tcttgaaaat cacgaatccc
    18301 agtagcagta cgagtaatcg gtggcacaag accaacccgt tcataatacc gaatcgtatc
    18361 cgctgaaatt cccaacaaat cactggcaga tttaatattc acacgctaac ctcctctcat
    18421 ttcattcact gaattcacgc tattctaact cacttatctt attatataac tttgagttga
    18481 ctccaagtca agcattttgt atctaatcga aaaattttta tcattaacaa tcatatatat
    18541 ggagtttata caatgctctt ctcttaccaa tttttgaaat atttaaataa taaagaaatt
    18601 actctattcc aaataagata gtacccaagt atttttcact tttttctaat gttggtgctc
    18661 ataactccgt tccaagtcac acacctttcg cttatgggtt tggtgagttt ctaggtcagc
    18721 atcaatttca atggtaatat tttgaaaacc acaatctttt aggaaaattc gaatagactc
    18781 tttacaagtt tccatatgtt ccatttcttt tagacaaaca tggacaatgg catttttttc
    18841 caaagcatcc atagtccaga gattaagctg attaaggctg accacattgt ccaatcgctc
    18901 caggccactc tttacttgct tgatatcaag accttctggc acagcatcca agaaaatctt
    18961 gagtgtagac caaaaacgtg gaagggcttt tgaaagaata aagaaagaaa tgacaaggga
    19021 caaaagagga tctaggatat accagtccgt aaatcgaaga acaatcgcca tcaggataac
    19081 agctacccac cctagcgtat cttccagaaa atgcagactc agaatagact cattctttgt
    19141 ctttccctta ccaaccacca gactcgctaa cagattgata gtaatcgcaa taattcctaa
    19201 ccagagaatc ccctcatcat tgactggttg cggatgcaaa atcttcgtga cattttccaa
    19261 aatgactaga acagagcctg ttacgagaat cacagctgtt accaaggctc ctagcaggct
    19321 aaaccgctta tagcccaagg tgtactgatt gtcttcttca cgattggaga ttgtttctag
    19381 aaaagctgat attccaattg caatcacatc tcccaagtca tgcacagagt cagcaagaac
    19441 agcgctagaa ccaaatactc cacctgcaat aaactcaaca atggcataag tcaaatttaa
    19501 gaaaaaagcc acccaaacag catattttgc cttcatattt ctcattcctt tgttataata
    19561 ggtttatgaa caccttgttc attatcatta tccactaaaa ctcaaagaaa ggatagaagc
    19621 aaaacgtcag ctttattcag ttctgaacaa tttgccttaa gtgtccatat gactaacatt
    19681 gaccgccgta tcagcaaaac caaaaaagcc atctatcaag cttttataca acttttgaat
    19741 gctaagggct acgaggccac tactgttcag gatatcatta atctcgcaga tgtgggaaga
    19801 tccacctttt actgtcacta tgagagtaag gagcttcttc tggaccagct ctgccgctac
    19861 ctcttccatc atctttttga aagagagcaa gccatttcaa ccgaggacta cctcgcccac
    19921 ctctttctcc attttcataa aaaccaagac catatcacca gtctgctatt ttccaaaaat
    19981 gactacttcc tccgtcaact ccataaagag ctagaacacc atgtctattc cgtgctagct
    20041 gataatttaa aagaagccca ccctaatatg cctacttctt acctccaaca cttggtcgtg
    20101 tccaacttta tcgagacatt gacctggtgg ctcaaaaaag gtcaagattt cacagaccag
    20161 gaagttgtcc aattttatct agaccttctc attcctaaaa attgaatata gagtaaagct
    20221 tcagttgtct tatttctagg ttactgagtt ttttatcttt tcaacaacaa aagaggaccc
    20281 gccgatcctc tttttcatac tataaatcct tgattatcaa ctatatctgt tttaatcaaa
    20341 atctcaaaac agcactttca aacatctttt cctagttaag taaatcagta ttctgcttag
    20401 ctgccttgct ccattgatac caaccaacta gactgttaat gagataaatt agatatttcc
    20461 cttgaatttg caggctttct ccccaccaga gatagattga aaaaacattg gtagccgccc
    20521 agaatatcca ctgttcacgg taaacagctg tcatgaggat ttgccctacc ccattggttg
    20581 catctgtgat tgaatcacga tagggacgat tggcaccaat agactgataa atgaagccaa
    20641 aggccaacca ccaaagcaca ctaatggaaa gatactttgt ccagcccttg ccgtccagtt
    20701 tacgcgcgac aaactcctgc ttttccttct taaactgtgc ctgataaatc caaactagaa
    20761 gtccaattgg ctgcatgact gtgaagtaaa gtgtcgtcag cacctcacca taaaagcctt
    20821 tctgtagggc caaaataagg taaataacag agttaatcaa gccaaaaaga taattacttg
    20881 ctcgaccttc cgatacaaag attacacaga taatccctgt caagctacaa atcatcccaa
    20941 tccagtcaac aatacgatgt tcgtaaacca actccagcca gagaggaaaa cttcctaaaa
    21001 ccagcaaata aatccactgg gcaaaactac gatgggcaaa gaggtcatcc cagatagcct
    21061 tcatagttcc tgaaaatcct aaatcagcca tagccgcaac catacgacgg taaccacctg
    21121 acatttcacc tagggttgtt ttgatatttt caattttctt ttgcaaataa gtatgcatca
    21181 tttctccttt tgtttttaaa gagccgtgtc tggatagact ttcggacgca acgctctatt
    21241 agataatgaa ctgcctatac acaagatttc taaccttagt cgacatgagc tgaaacctct
    21301 tatttgttaa gtagttcaca aaatattata cacctatttt atgaatagtc aactgtcttt
    21361 acagtaaaat tttagaaaat catgaaaatt ttctctttct ttccatttta agtgacattc
    21421 agtcattctc acatcaaaaa agcccagacg aaattgtctg agcattcttt tatctagtcg
    21481 tttaaggaag ttgagttcag tatgtttaaa gtctctgtcc catcatttct tcaacaaacc
    21541 ttgttcttgg agaaactcct tggctacttg ctttgctgac ttgccttcaa caccgacttg
    21601 gtagttgagc tggctcatct ggctttctgt aatcttacca gccaatgtat taagaactct
    21661 ttccaactct gggtgtttct tgagaagagc ttctttcatg agtggagccc cttgataagg
    21721 tgggaagagt tgcttgtcat cttccaagac ctgtaaatca taacgctcca attccgcatc
    21781 agtcgaatag gcatccgtga tttgaatatc ccctgactga attgcctgat agcgaagagc
    21841 tggctgcata gtcgccacat tgagattgag accatacatt gattgcaagc ccttatttcc
    21901 atcttcacgg tcgttaaact caagcgtaaa gcctgccttc agctgccctt ccactttttt
    21961 caaatccgaa atggtcttca agccatattc ttgagcaatc tttttcggaa cagctacagc
    22021 ataggtattt tgataagaca taggtttgag ataggctaga tgatcctgct tggcaattcc
    22081 atcacgcgcc acttgataaa cctgctctgg ctcatgactc accttgggtg atggttgaag
    22141 caaactttca gtcaccgtac cggtaaattc aggataaatg tcaatatcac cttttttcag
    22201 agcttcataa aggaaacttg tcgtcccaaa attcggttta acagtcgcag tcatgctggt
    22261 attttcttca atcagcaatt tatacatatt agccaaaatt tctggttctg ggcctatttt
    22321 cccagcaata atcaagtttt ccttctcttt ttgagccaaa agggctggac tataagacag
    22381 acccagtaat aaagccatca aggcaaaacc agagaaaatc gtccgcaatt tcgctttttc
    22441 cattactttt agtaggaagt taaaggcaat agctagcact gcagaagaaa gggccccaat
    22501 caaaatcaaa ctggcattat tacggtcaat tcccaacagg ataaaggaac ccagtccccc
    22561 tgccccaatc aaggtcgcca aggtagccgt accgataatc aagaccgctg ccgtccgaat
    22621 tccagacatg ataacaggca tggcaagtgg aatttcaaac ttcttgagac gctcccatct
    22681 ggtcatccca aaggcaatcc cagcctcttg aagactcgga tcaattccct tcagcccagt
    22741 aatagtattt tgtaaaatcg gaaaaatcgc ataaatcact agagctgtca aagccggcaa
    22801 ggtcccaatt cccatcaaag ggataaagag ccccaacaag gccagagacg ggatggtctg
    22861 gaaaatacct gcaatctgca agacccagtc ggccagcttc tcatgatagc gaagaaaaac
    22921 agccaaggga atcgcaagca aaatagctag taacaaggtc aaaagtgaca actgcaaatg
    22981 ttgagataga gctgtcaacc aatcactaaa acgatcctga aaagttgcaa ttaaattagt
    23041 catgaacact acctccaaac aagtctgcta caaagtctgt tgcaggcact tttaaaattg
    23101 tctcgggatt cgctacctgg cgaatttctc catcctgcaa gacagcaata cggtccgcca
    23161 acttcaaggc ttcatccgta tcatgggtta caaaaatcgt tgtcatccca aactctttat
    23221 gcaattcttt tgtcagaacc tgcaactgtt ttctcgaaat agcatccaag gccgaaaagg
    23281 gttcatccat gaggaaaatc ttgggctgac caatcatagc tcggacaata ccgacccgtt
    23341 gctgttctcc accagataat tcactaggta agcgatgccc atactcggct actggtaaac
    23401 caaccttagc caaaagctct tctgttttct tcgtaatttc ttccttgctc caccccttca
    23461 tttcaggaat gagagcaata ttttccgcaa ctgttagatt tggaaaaaga gcaatagcct
    23521 gtaaaacata accagtagaa agacgaagtt cacgctcatc atagtctttg atgcgcttcc
    23581 catccatata aatatttcca tcagttggtt ccaaaagacg gttaatcatc ttgagcatgg
    23641 tcgtcttacc tgacccagaa ggccctacta aaaccataaa ttccccatcc tcaatctgta
    23701 agttgacatc tctcaagaca tccttttctg tgtagcgcag tgctacattt ttgtattcaa
    23761 tcattctttg tcctcaattt aaaacttccc tcgattagtc aagtcttcta ccttaggcat
    23821 gacttcctta ttatcccaat gctccacaat tttcccgttc tctaaacgga agatatcgta
    23881 ctgggcataa gcaacgccat caatctgagt ctgaccatag ctaaccacat agtttccttg
    23941 tcctaagagt tggaaaacaa agtcaaaagt gacactatat tcagccacat agtttttata
    24001 agcagcactt ccttgtccaa tctcatgatt atgctgaatc aaatcgtctg ccacataatc
    24061 actccactgc tctagctccc cattttggaa aatttctgtc aagaaacggc gaaccagctt
    24121 tttattttct gctttcttat ccaaatcctt gatttcaaaa tctccaaaaa tttgatctag
    24181 ttgatcattt tcaggtgttc gatagtagtc aatgacatcc caatgctcaa caatacgacc
    24241 attctcatcc gcacggaaag tatccgtcgt cacccattga gcttctccac cattcagata
    24301 ttgatgaaca tgaacaaaga ccagattgcc atcctcaatg gtgcggacaa tcttaatctg
    24361 acgctctgga tgacgctcaa agaaatctgc aaagaaggct gcaaatcctt ctttcccgtc
    24421 aggaacacct gtcgaatgtt ggatataggt atcccctaca gactgggctt gagcctcagc
    24481 aactcgtccg tcttgaatgg catggatgta taggttgtga gcatttttca cttgttgtga
    24541 catattctaa acctcatttc ccttctcttt caaattcgcc aaaattcttt cttgaaaacc
    24601 ttcaaattgg tgaatttctt cctctgaaaa tcctttgtaa aagatagtat ccaatttctg
    24661 actgacacga tgccccactt ctttctggga cttgcctaac tccgttaaaa ctaaatactt
    24721 cttacgcttg tcttttccac acggactaac aattacaagc ttttgttcct ctagcttttt
    24781 tatcatagtc gtcagcgtat tattcgcaag cccagtcgca agcgcgatat ctgtcgcagt
    24841 tgcgcagcca gtttcactat tccataaaac cgctaaaatc ttgccctgtt cacccctata
    24901 aagagcctca ggatcttgac tcagtaactt ttgaaaaatc cgcccattca acaaacgaat
    24961 atgatgggct agcaaatgac tatctttcat aacacctcca atttatttcg atgtcgaaat
    25021 gaataaaata attgtaacac tcatcgttct aactgtcaac tatttcgatt tggaaataat
    25081 ttttgataat tattcacacc accatactcc ggctcaacta acttttaacg agagtttcta
    25141 aattccttcg tcctccagtc tacaaaagcc ttccatccgt actatcctat tttttatgag
    25201 gggacacatt tttcctatca gatcatttat tttaaagata gaagtaaatc ataattgctt
    25261 ccatctgttc ttttatagta tattgaagtt ggactagggc actgtatctt ctaaaacatt
    25321 gatagaaagc gatttgaatt tcccaatcaa tttgttcgtc tttatagcat ttcgaaactg
    25381 gaataggaca ccatgactgc taaaagattt ctataaattc atttgatttc cctaatcaat
    25441 ttgttcatat cttatttcat tccgctataa tttcacctta ccctatcttt ttcgtagcac
    25501 ccttcaaaca gcctatcccc taccgtttga cgattcctca cttcactcca cttccattac
    25561 agaagtttct tcactactat ggtctcggct gacttctcat gattccttgt tactactatt
    25621 tgaacgctca cgagataggt cttataaaaa atgctttgat ccacaatgga atcaaagcat
    25681 tttaaaaagt tcctcataca taagtgcaga agtcgcagtt cctctgtact tggcttcttc
    25741 tcttttgata aagcgagcca agttgagcaa ctcaggtgct ggatgtttgg gatttaggag
    25801 caattcacga ttgaccaggc ctgagagacg aactgcctgc aattgctcat ttgtagtagg
    25861 cagtttttta gtagtctcta ggagagcagc aactaaatct tcactcaaat catgtcgagc
    25921 atgattgtaa agatctttta taaggctttc taggtttggt tctgccatcc ctaccacctc
    25981 ccttatggtt taataatgtt taatcaaatc aaccgttgaa cgatccaatt tcttcaccaa
    26041 ggcttgtaag aaagcttgcg cttctaggaa gtcatccatt gcatagaggg tttggtgaga
    26101 atggatataa cgagcgcaga caccgatagt tgttgatggg acaccaccat ttttcagatg
    26161 agctgcacct gcatctgttc cgcctttacc acagtagtat tggtacttga taccagcttc
    26221 ttcagccgtt gtcaaaagga aatccttcat ccctgggaga agcaagtgac ctggatcata
    26281 gaaacgaatc aaggttccat ctccaatctt gccttgacca ccgtagacat cacctgctgg
    26341 ggagcaatca actgctagga agacttctgg gtcaaacttg gttgtagagg tatgagcgcc
    26401 acgcagacca acttcttctt ggacgttaga acccagatag agttcattgc cgagtttttg
    26461 acctgataaa gcttcagcta gctcgcttac catgaggaca ccgtagcggt tatcccaagc
    26521 ttttgagatg atattttttt cattggctgt caaaattgca gaactatctg gtacgatggt
    26581 atcaccagga cggatgccaa aactttctgc ctcagccttg tccgcaaaac caccatcaaa
    26641 aacgatatcg gcaatggctg gcatggttgg tccccccttt ccacgagtca aatgcggagg
    26701 aacagaacct gaaatcacag gaatttcatg accatcacga gtcaagagtt tgaaacgttg
    26761 gctgctaacc accatggggt tccagccacc gatttctacg acacggaagg taccatctgg
    26821 cttgatttcg ctgaccataa aaccaacttc gtccatatga gaagcgacca agacgcgcgg
    26881 tgcatccaca gcttctgaat gtttgatacc aaaaatacca cccaagccat ctgtcaccac
    26941 ttcatccaca tgcggtgtca acttttcacg aagataagca cggacaggcg tttcatgacc
    27001 tgagactgca gcaagttctg ttacttcttt aatttttgaa aataatgttg tcatttcagt
    27061 tccttctttc tttcatccat tttaccactt tttataggag aaggatagtg ggaaggtgga
    27121 tttctaagtt agtatcttag tcctgctcta tcttagaaaa ggatagtatt ctcttgcatg
    27181 tagtgcaaaa tctagtaaac attccaaaat taactcgaat atttatttcc aaacaaaaaa
    27241 caatacacca tcaaagttgt ttggattttt catgaaattt acagaaaata gttgacttcc
    27301 ctttcttctt tctttaaata tatagttggt tgagtttgga atagtacgct gtagctgcta
    27361 aaacatttct agaaattaat ttgactttcc taatagagtt gttcatatct tatttcaatt
    27421 tactatagta caaaactaga aaaggaaaaa atcatgacca aaacaattct tgttacaggt
    27481 gggactagct acattggctc ccataccgtt aaagctcttt taaatgctgg ctataaggtg
    27541 catgtcctcg ataatctctc cacaggaaat cgagcggctg tggatagtcg tgctagcttt
    27601 aaagaactgg atgtttatga tgctagcgcc ttaaaggctt acttaaaaga aaatcagatt
    27661 gatgctgtcc tccattgtgc aggtgaaatt gttgtgagcg aaagtattga aaatccaagt
    27721 aaatacttca ctgccaacgt tgctggtatg aaccaagttc tcaaagtctt atctgaagtt
    27781 ggcattcaaa aaatcatgtt ctcttcgact gcttctctct atggaaataa ctgtattgac
    27841 aagccggtga ctgaagatac cctgctcgac cctatcaacc cttatgcaga gacaaaactg
    27901 atgggcgaac gaatgattta ctggatggcc aatcgctacg actggaaata tgttattttc
    27961 cgttacttta atgttgctgg ggctgaaatg gatgcttcaa acggtctgcg tgtgaaaaat
    28021 ccaactcaca tcattccaaa tatcaacaag accgcattgg gacaaaatga tagcttaaaa
    28081 atatttggag atgactacga tacacgtgac ggttcatgta ttcgagatta catttatgtc
    28141 ttggaccttg cataggctca tgttaaagga atgaactacc tatttcaaga agacagttct
    28201 tctcaaatct ttaacttagg aactaaaaaa ggctatacta tcaaagaaat ctttaaaact
    28261 gctgaagaat tactaaatta aaaaatccca cacgagattg ttgctcgacg tgctagtgac
    28321 ccagccagtg tcctagcaga tgcttcaaaa gctaaacaat atcttgattg gacggctagc
    28381 tactccctcg aagatattat tttatcagat caaggtaaaa cattttagaa taggataaca
    28441 aaggagaaag tcttgttgct ctctccttgt tgtatttatt aaattgtcag aaattttatt
    28501 gacagattag aaaaataatg ttacatttat atccgcaggt atctttcgat accaaatcta
    28561 catgaaagga cggggtatga aactttctca ttatttaatt ggcttacttc tactcctagt
    28621 ctttctctct attagcattg ggaccagtga tttttcatgg ggaaagctat ttgatttcga
    28681 ccagcagacc tggctcctct ttcaagagtc ccgtctccca agaactatca gtattctcct
    28741 gactgcctct agtatgagta tggcaggcct tctcatgcag actattaccc aaaatcagtt
    28801 tgctgcaccg agtacagttg gaacgactga agccgccaaa ctgggaatag tgctgagcct
    28861 ttttgtcttt ccatcggcta gtctgaccca aaagatgctc ttcgcttttg tttcatccat
    28921 cgtattcacc ctcttcttcc tagcctttat gaccattttt actgtaaagg aaaggtggat
    28981 gttgcctctg attgggatca tctatagcgg gattatcggc tcagtcacag aagttatcgc
    29041 ctatcgtttc aatctggttc agagtatgac tgcctggacc cagggctcct tctccatgat
    29101 tcagacccat cagtatgagt ggctcttctt aggcctcatc atcctgataa ccgtttggaa
    29161 attatcccaa accttcacca tcatgaatct agggaaagaa accagcgaga gtttggggat
    29221 ttcctactcc ctacttgaaa aactggccct ctttctggtg gcgctaacga caagcgtcac
    29281 catgattacc gtgggtggcc taccatttct cggagttatc gttcccaatc ttgttcgcaa
    29341 gcgctatgga gataatctaa gtcaaaccaa actcatggtc gcactggttg gtgccaatct
    29401 agttctggct tgcgatatcc tatcccgagt tctgattagg ccctatgagt tgtctgtcag
    29461 tctcttgcta ggaatcatcg gtagtctcgt ctttatccta cttctctgga gagggggacg
    29521 aaaagatgca gactaaaagc aaacatacca agctcttctg gattctcatt attcttgcca
    29581 tcggagcttg tcttctctac ttttggccca tcactcactt gtcagccttt gcttggaagt
    29641 tgcgttccca aaagatcatc gtttatctct tggtagccat cgcgactggg atttcgacca
    29701 ttagttttca aaccctgacg gaaaatcgct tcctgacgcc tagtattttg ggaattgaat
    29761 ccttctacgt cctactacaa accctactac tggtttttga aagcaagttt cttcaacttg
    29821 gcaagtcccc tatcttagaa ttcctagtct tacttcttgt ccagtccctc ttctttctcg
    29881 ccttacaagg ttacttgaag acactgatga agcaagacct ggtcttcatc ctgctgatct
    29941 gtctagcgct cagaagtctc tttcgaaata tcagcacctt ccttcaagtc ctaatggatc
    30001 caaacgaata cgataaactg caaaatagtc tttttgcctc ctttcaacat ctcaacactt
    30061 ccatcctagc catcggttct ctgatcatcc tcgctttgac aatctttttc tttcgaaaag
    30121 cagtcgttct agatgtcttg cacctgcaaa gagaaacggc tcagatattg ggactcgatg
    30181 ttgaaaaaga acagaaagaa ctcctctggg gaatcgtgct tttgacctca acggccactg
    30241 ccttggtagg acctatggcc ttcttcggct ttatgctggc caacctcacc tacctgattg
    30301 tcaaagacta tcagcacaag ttactcttta tagtggccat tctggttgga tttattagct
    30361 taaccttggg gcaagccttg attgaacgag tctttgcact ggaaattcgt atcagtatga
    30421 tcattgagag tgtgggtggc ttcttattct ttatcttact atataggagg tctcgtcagt
    30481 gaaactggaa aacattgaca aatccattca aaaacaggat attttgcaag gcatttcgct
    30541 tgaagtcagt cctcaaaaac tgacagcctt tattggtcca aatggtgctg gaaaatcgac
    30601 tctcctctcc atcatgagca gactaaccaa gaaagatcag ggagttctca gtatcaaagg
    30661 acgtgaaatc gagagctgga attcgcaaga actggcccaa gaactaacca tcctaaaaca
    30721 gaaaatcaat taccaagcca aattgactgt tgaagaactg gtcagttttg gacgttttcc
    30781 ctacagccga ggtcgactta gatcagaaga ctgggaaaaa atccgagaaa ctctgaacta
    30841 tttggaactg accaacttaa aagaccgcta catcaatagc ctgtcagggg ggcaactcca
    30901 gcgcgtcttt atcgctatgg tactggccca ggatacagac tttatcttgc tggacgaacc
    30961 actcaacaat ctcgatatca agcaaagcgt cagcatgatg cagattcttc gacgactggt
    31021 ggaggaactc ggcaagacca ttatcatcgt cctccacgat atcaacatgg ccagtcagta
    31081 tgcagatgaa attgtcgcct tcaaggacgg tcaggtcttt agcaagggaa gcaccgatca
    31141 aatcatgcag gctgacctgc tcagccaact ttatgagatt cccatcacgc tagctgatat
    31201 caatgacaaa aagatctgta tctatagcta gtaacataaa agctcaagtt agagaacctt
    31261 cagtctctta atcaataaga tcaagagact ccctaaatcg ttatcacatt ttaaaaagga
    31321 gaaattatga aaacatccct taaactttat ttcactgccc tagtggccag cttcttgctc
    31381 ctacttggtg catgtagtac aaactcaagc actagtcaga cagagaccag tagctctgct
    31441 ccaagagagg taaccattaa aagttcactg gacgaggtca aactttccaa agttcctgaa
    31501 aagattgtga cctttgacct cggcgctgcg gatactattc gcgctttagg atttgaaaaa
    31561 aatatcgtcg gaatgcctac aaaaactgtt ccgacttatc taaaagacct agtgggaact
    31621 gtcaaaaatg ttggttctat gaaagaacct gatttagaag ctatcgccgc ccttgaacct
    31681 gatttgatta tcgcttcacc acgtacacaa aaattcgtag acaaattcaa agaaatcgcc
    31741 ccaaccgttc tcttccaagc aagcaaggac gactactgga cttctaccaa ggctaatatc
    31801 gaatccttag caagtgcctt cggcgaaact ggtacacaaa aagccaagga agaattggcc
    31861 aagctagaca agagcatcca agaagtcgct actaaaaatg aaagctctga caaaaaatcc
    31921 cttgcgatcc tccttaatga aggaaaaatg gcagcctttg gtgccaaatc tcgtttctct
    31981 ttcttgtacc aaaccttgaa attcaaacca actgatacaa aatttgaaga ctcacgccac
    32041 ggacaagaag tcagctttga aagtgtcaaa gaaatcaacc ctgacatcct ctttgtcatc
    32101 aaccgtaccc ttgccatcgg tggggacaac tctagcaacg acggtgtcct agaaaatgcc
    32161 cttatcgctg aaacacctgc tgctaaaaat ggtaagatta tccaactaac accagacctc
    32221 tggtatctaa gcggaggcgg acttgaatca acaaaactca tgattgaaga catacaaaaa
    32281 gctttgaaat aagccacttc aaactcaatt taatactcaa tgaaaatcaa agagcaaact
    32341 aggaaactag ccgcaggttg ctcaaaacac tgttttgagg ttgcagatga aagctgaagc
    32401 ggtttgaaga gattttcgaa gagtataaaa ttttgacatt ctaaaacgca tcctatcaag
    32461 tttactcaaa cctgatagga tgcgttttta tcattttgaa atctatttac cccacctcat
    32521 tccccttctt gatttcgttt aagggaaaag tggtctggaa cggggtcctt acctgttttc
    32581 gaccagggat gacaacgtaa aatccgagcc aagcccatca atactccctt aaaaccatgt
    32641 ttttcaatag cctgaatcat gtagttggaa caagtcagat caaagcgaca agagggtgga
    32701 aagactggtg agataaaacg ttggtaaaag cgcacaggcg ctattaaaat tcgtttcatt
    32761 atttcttagt tacagccatg gtgtgtagtt ggctgatttc ttttttatta agacgacggg
    32821 attctcctgg acggagtcct gtcaagtcta ggtgtccgaa acgagtccga gacaacttat
    32881 ctacttggag accaacagct tcaaacatct ttttaacctg atggttacgc ccttcatgga
    32941 tgatcaactg caccacagag cgatttttga ctgggtccac tttgagaatt tcataaacag
    33001 ctggcttggt tttcttacca tcaatctcaa gaccacgggt caaggggcgg agattgtcct
    33061 tattggccac acctttaaca cgcgcgacat aaaccttgtc aatctcatta cgagggtgaa
    33121 tcatctcgtc tgtaaagtcc ccatcattgg tcaaaatcaa gacacctgat gtatcccagt
    33181 ccaaacgacc cacagggtaa atacgctctt tgacattggg caagaggtcg acaaccgtct
    33241 tgcgaccctt atcatctgtc acactggaaa tcacaccgcg tggtttgtta agcagatagt
    33301 agaccttttc ttcgttatag ataggttgac cttcaacttc gaccttgtcg cctgacttga
    33361 tagtggttgc tagttcacgc accacttggc cgttaaccgt caccaagcct tgcttaatca
    33421 gctcttctgc ttttctccta ctggccacac ctgcgtgggc aatatactta ttgattctca
    33481 tcttcttcta tcctttcacc aaataattgg ctttcttggg cttgaatctc aagctcatca
    33541 atcactggta attcttctaa atggtttatc cccatgtaat ctaggaaata atccgtagtc
    33601 acatagaggt tggggcgccc caatacttcc tttttcccgt cttcctttat caggtcaaaa
    33661 gcctgcaact ttgccaaggc tccactcgag ttaactccac ggatggcatc aatttctatc
    33721 cgcgtaatcg gctgtttgta ggcaataatg gacaaggtct caagggcagc ccgagacaag
    33781 ctctggttga taggcgcctt agagtattcc ttcaaaatct ctgcaaattg aggcttggtc
    33841 accaatctat aagcaccact tgtctcaatc aaagccaaac tggaatctgg gtccttttca
    33901 tacttctggg ctaattttcc taaactttgc tggatgcctg tcggtggcag agagaggagt
    33961 tcagctaact ggcggacccg aatcccatct tcacccgcta caaacaagag cgcttctatt
    34021 tttgctaaag tactcatctt tcctctctat caagtctagc tttgaggcac ttgactttct
    34081 tccttctttt ccatgagata gatatctccg aaactctcct cttgcacgag gatcagctcc
    34141 tgggttttga ttaactctag ggttgccaaa aagagggtga tgacctcttg gacattctgg
    34201 gcttccttga acaaatcctg caagcgcaat tgatctcgtc caatcaagga ctctttcacg
    34261 ataatcatca tgtcctcaat cttatactca tcccgcaaga tagtcgtgtg attttgtgca
    34321 aactcctctt ttttcttggc taggatattt gaaaaagcca aaaagaggtc aatggtcgtc
    34381 ttgtcatgca caagctccgc atcttcgtaa atcaactctg tcggcgcttt ggaataatac
    34441 tgggcccgtt cttggtgctt ggattccaag tgctcaccca agagcttgaa cttgcgatat
    34501 tcttcgattt gagagaggag gtcctgctcc aggtcatccc ccaagtctgt cacttctgct
    34561 accttcggaa ggagtttacg actcttaatc agcatgagct gactagccat gaccatgtac
    34621 tcacccgtca cttccagacg catggcctgc agggttgaga cataggctag atactgttcg
    34681 atgacttccg taatgggcac atcgtagata tccatctggt acttagaaac cagatgcaag
    34741 agcaagtcca agggtccttc aaaatctttt aatttaatat ccattatcta tatttttcta
    34801 aggtcaggac tgtttttaat cctaattttt ttgcaatttc gtacaaatcg accttgtttt
    34861 ctatttgtct tagaataaac tgttcacgta agacttgagc tgataaggat ggaaaacctt
    34921 tctccttgac aaaagattct aactgacgaa aggcccactg acgagaatag ggtttctctc
    34981 ctctttcaaa aagataggtc tgccccatca agggttccaa ttctgaaagc aaggccgtgg
    35041 gaatggtgac aatcctctgt tgggaagcct tgctgattcg caacacctga aaatccagat
    35101 tgatgtccgc aaccttgatg gctaaaatct cactgggcaa gagccccatt tctaggatta
    35161 agagcgctag caagcggccc tctggatggt cgctttcctg ccaaaaagag tctaggtata
    35221 gaatctctgg cttttccgtc ttcttttcag cttgtttggc taattccaag cggtaaaagc
    35281 tgtccacctc tcctttttga tagagaaagt atagaaattg gttacaggac gaaatctttc
    35341 gcttctgggc gctgattttt agattggcta gctgggcttg gtaaatcttg agactggtct
    35401 cagaaatccg ctcacctacc atgtctaaaa attgctccaa atcatactta taggactgct
    35461 tggaattgac agacaagccc tgcttttcct ctaaaaaggc tgaaatcctg tctctcattg
    35521 gcatcgaatc tcatattcct tactaaagtc atgcaagagg gcattaacgg ccttgaggat
    35581 ggacttgcgc gtaataatcc cttggaaaat accctctgaa tccacaaccg gtaagaagga
    35641 ctcatctact agcttgtgca agacctccgt aatggtgaaa tcaggcgaaa caaccgctac
    35701 gtccgttttt gtcatatgaa cgatatccgt atccgccatg atttcttggc tcaagtcatg
    35761 ctccatctga taagccataa tatctctgag tccaatcgtc ccaacaaact gtttttcatc
    35821 tgtcacaacg ggaacacggg tataggtcat ctgactgagc aagagggtcg catgatccgc
    35881 attgtgggta tcaatcaaca cagctagatt tttagcaggg gtcaaaaaag tttcctcctg
    35941 ccccaacaag aaagtctcaa actccttggc aatcatcggc taaactcctt ggacaaacct
    36001 ggatacacct cgtgatctcg tgtcaaaaag tccactttga agtaactatc atcaatctcc
    36061 acacgagcat agagacattc tctgatggta cctcgtggtt gactgataga acctggattt
    36121 agaaagagga tcttgccttc catccaagca cttggcacat gcaagtgacc atagaggcag
    36181 atagcggcct cttcctcctg agcccagtag tccaactttt gaaagttgaa attgatgtca
    36241 aacaagtgac catgagtttg gataatcttg gtcgaaccaa gctcagtcac cagacgttct
    36301 gggtagccgg cgtagaagtc catgttccct ttaacaacgc ggatgccctc ccaaagtgga
    36361 gaatccggac gtagttcaga atcgccgtta tgaaaaacgg catcgacttt gcccacatag
    36421 cgatcacgga cttcttccac aatcaagcta tcgccatggg aatcgctcat tacaatgatg
    36481 gtttgctttg ccatgatgga aatacctcca aaagtttctt aacggctaag gcacggtgag
    36541 attgactatt tttttcttcc agggttaatt cagctgatga ctcacctgtc tctcctacaa
    36601 ggaagagggg atcatagcca aagccatttt cacccttagg ttcaaagtta atataacctg
    36661 accagtctgc ttcaacaact aaactttcct tatttgggct ggctacgact agggttgtgt
    36721 ggaactgagc cgagcggtcc ttgagttcaa agaccatggc caattcgtgc aagagtttgg
    36781 cattattttc acggtcagtt gctcccacac ctgcgaaacg agctgaccag acgcctggta
    36841 agccaccaag gacatcgact ttgagaccag aatcatctgc caaaaccatc ttgcccgtta
    36901 attgagaaat ggtttctgcc ttaaggcggg cattttcttc aaaggtcata cctgtttctg
    36961 ctacttcagg caggtcaggg tagtcattaa gattttccac atcgtagcct aacttatcaa
    37021 agatagctcg gaattccttg gttttacctt cgttacgagt cgcaatcaac aaggtttctc
    37081 tgaccttgct tgtctcaaag aaatcatgaa cattgacccc ttctttaggc aattctacat
    37141 acaagagttg cccattttca accaagagca gggccccatg acgttgaata acttccaagt
    37201 ttcgtcccat ttcttgatta agcatgtcta ccacaaagga gaacaaacgg taggtagaac
    37261 catagcgtaa aacagtaact gaaagcggga aaccatactc ttcatctcca aatattttgg
    37321 agaattcaaa cagaggaaaa tctgtcttat agtcgctcaa actgttaacg ccaccaaaaa
    37381 tactataaga cccaacatac cagtcctggt catccttata ttcataaatt ttatttgtca
    37441 taattctaca tgctccacat gaatctcttt ttccagccat tcttcaccaa tttgtgcaaa
    37501 actttggcta ctggctgttg tgtaaaaacg gtgatggagt ggtccagcat cgcgaccacg
    37561 attgatttca aaataattga gtaagactga gatatcccgt acgcactctg ccccactatc
    37621 gatgagctga acctttggcc ccatcacatt ttggataata gggcgaagga gtggataatg
    37681 agtacagccc aaaatcaggc tatccacctt tccaaccaag ggacgcaggg tttcatagac
    37741 caccttcttg gtaacactgg ttgacagggc acctgactca accaagggag caaacttggg
    37801 acaggccaag ctctccacct gtaagtcggg atccagatca tggattttct gacggtatat
    37861 gtctgattgt accgtcatgg gcgttccaat cactccgatt ttcccacctt gactggactt
    37921 gatggctgcc gaagctcctg gcaaaattac acccaagaca ggaatatcta gttgagcctt
    37981 gatttcttcc cagacgaccg cagtcgcagt gttacaagca atgacaatca ttttgacatc
    38041 cttggtcaag agaaagttga ccagctgcca agtatattca cgaatttgct cagcaggacg
    38101 ggggccatag ggcgcccgcg ccgaatctcc aatatagacg atttcttcat ggggaagctg
    38161 gcgcatgagc tcgcgcacaa cggtcaagcc cccgacaccc gaatccaaaa aaccaattgg
    38221 tcgattatcc atacagtctt ctttctagtc ttttttctga ttgatcgttc attatgattc
    38281 cttgtactga acgaactgac cgacagcttg atagaatgtc aactacctct ctcttaatca
    38341 taatcaaata tcaatagaat gcaaggaaaa agtctcatcc ctaaaccata accaacatag
    38401 tgagaagtct ggaactttca tcccagactt ttccttattt attttttctt tttattgtta
    38461 gcaagggctg ccttttgttg gcggatgatt tggtggtaaa cttgttgtac cttagcttcg
    38521 cttggttttt gaccatttgc actcaaaaga gtacgaactg cttcagcatt caaacgtggg
    38581 ttgtcagcaa attctttttc gatttgctta cgaaccaagt acattcctcc aagagctcct
    38641 cctagaaaag ctagcacaat caatacaatt gctaaaagta aatccatcat ttttctcctg
    38701 tttctttttg aatccctttc attataccac aaactagcca ccctgaccag tttgttctac
    38761 actttcaagg atggaataga cagcaaaaag aaccctgctt ttctgaaaaa gaggtctcct
    38821 tgctaagttt atactcaatg aaaatcaaag agcaaactag gaagctagcc acaggttgct
    38881 caaagcaccg ctttgaggtt gcagataaaa ctgacacggt ttgaagagat ttgcgaagag
    38941 tattaattta cataaatagc cagtgtttga tagggtttga gtagaatttt ctcagacact
    39001 tctgcatctt catagtttga tatcaaaatc tgtccatttt ggtagactgc tggcaagtcg
    39061 atttccactt ctttagcata aaagttattg agcactagta acttttgatc ctcaaactgg
    39121 cgttcaaaag cgtagacttg tttgctatct tcaaaggctg gtttgtaact tccttctgaa
    39181 atgattggca tttccttacg cagtcgaatc aagtcttgat agaaggtaaa aatcggaccc
    39241 tggatttcat tttctacatt gatgtcttta taggatttac cagctttcaa ccaaggagtg
    39301 cctgttgaaa atcctgcatt ttccgaagca tcccactgca tgggaatgcg tgaattatca
    39361 cgcgacttag cttgaataat ctggaaggct tcttgctgac tctttccttc ttctaagagc
    39421 atctgatagg cattaagcga ttcgacatcc acataatcag ccatagaatc atagtctggg
    39481 tcaatcatcc cgatttcctc acccatgtag atataaggtg tcccacgtga caggtgaatg
    39541 ctggccgcta gcatggtggc tccttccttg cggaagtttt gaatatcgac aaaacggttc
    39601 aaggcacgtg gttgatcgtg attgttccaa aagagggcac tccaaccgtc tttatcactc
    39661 atttccttac cccaactatg gtaaagactc ttcaactctt caaaatcaaa gggagccaag
    39721 gtccactttt gtccatcctt atagtccacc ttgaggtgat gaaaattaaa ggtcatggat
    39781 aattcctgac gatcaggcga cgaataaagg acacagtttt ccatggtggt agaagacatt
    39841 tccccaactg tcataaagct atcgtcagat ccaaaagtgg cttggttcat catacgcaaa
    39901 tagttatgaa cgatgggttt gtctgtataa gctggcttcc cttcattttc aggacagtcc
    39961 actgaaacct cgtccttacc gatcaaattg atcacatcaa atcggaaacc tttgataccc
    40021 ttgtcgcgcc agaaattaac aaccttgaaa agctccttac ggacattgga attgcgccag
    40081 ttaaggtcag cctgggtctc atcaaatagg tgaagatagt atttcccagt atccccgaaa
    40141 ggcgtccatg cagaaccacc aaacttagac tgccaatctg ttggttggtc ttggatgaag
    40201 aaaaagtctt gataatactt atcaccagct agggctttct gaaaccattc atgctctgtc
    40261 gaacaatgat taagtaccat gtccagcata aagtcaatct tgtgctcttt accgacacac
    40321 accatttcct caaaatcagc catatcacca aaaagaggat ccactgccat ataatctgaa
    40381 atatcgtaac cattatcccg ttgagggctt ggatagaatg gattgagcca gaccatatcc
    40441 acacctagtt tggctaaata gggaattttt tcgataatcc cacggaaatc cccaataccg
    40501 ttttcagtgg tgtctttgta agattttgga tagatttgat agactacttt tcctttatca
    40561 agtgtcatct gtttctcctt ttctgataaa agggaggaag cagtcttccg tccctatttg
    40621 tgctatttca attatactca atgaaaatca aagaacagac taggaagcta gccacaggtt
    40681 gctcaaaaca ctattttgag gttgcagata gagctgacgt ggtttgaaga gattttcgaa
    40741 gagtattaga ttcgtgtagc gaccatgaga gatgctccag cttggatcgt tgtcggataa
    40801 gttccgggaa tagtcgctgt ataagcatct tggttggtga tgataacagg agtttctgtc
    40861 accagacctg cagccttaat gacatccata tcaaaacgaa tcagttgctg accaactgta
    40921 acgtgatctc cttggactac aagactttca aaacctttgc catcaagacc tactgtatcc
    40981 ataccgatgt ggatgagcaa ttcaactccc tcgtcagaga caatgccgat ggcatgcttg
    41041 gtagggaaaa gaaccgtcac tgtcccatta actggagagg tcaactcacc ttggcttggt
    41101 tcaatgacca gaccttgccc catgacacct gatgcaaaaa taggatccgt cgcttgactc
    41161 aattctttca cttggccagt tagtgggctg ataatttcta ccgaagtaag ttctactggt
    41221 tcatggttca caaattctgc ttcttcttga gcaacgaatt ctgcctgcaa gttcgtatcg
    41281 ccctctgttt ttgtaaagag accagccttg cggaagaaga aagtcaagag cattggaaca
    41341 acaatcgcaa ctagcatagt tcctgcaaat ggcagcatgt attgaggttg aatagagaga
    41401 atacctggca aaccaccgat accaatagaa gccgcagtta cattaaaagt aacggataac
    41461 atgcctgcaa gggctgaacc agtcatccca gcaacaaatg gataaatata ttttacgtta
    41521 accccaaaaa gagctggttc tgtaacaccg agataggctg aaatggttgc aggaagtgaa
    41581 acctgagcct cacgctcatc atggcgatgc atgaaataat aggcaaacac ggctgagcct
    41641 tgagcaatat tagaaagagc aatcattggc cagagggcag tgccaccagc atccgcaatc
    41701 aattgtgtat caatggcatt ggtcatatgg tgcagacctg tgatgacaaa tggagcgtag
    41761 agggcaccaa aaattgcacc gaagagccat ttaactggac cagttaaacc tgccaagaca
    41821 actgatgaaa gtccttgtcc aattgtccaa ccgattggtc ccaaaacagt atgagccaaa
    41881 atcaaggctg gaatcaatga caaaaaaggt acaaaaatca tggaaatgat ttctgggata
    41941 tgtttgcgcc agaaaatttc aagataagac agactcaaac ctgcaagcaa ggctgggata
    42001 acttgggctt ggtaaccaat acgattaaca gtaaaataac caaaattcca aacccagttt
    42061 gctgcgatat cagctgctgg cgttgaagca accgcatagg cattgagcaa ctgaggcgat
    42121 accaaacaga ttccgagaac aattcccaaa atttggctgg ttcccatctt acgagaaaca
    42181 gaccaagtaa tccctactgg taagaactgg aagatagctt caccaggcaa ccagaggaag
    42241 tgattaaccc ctgcccaaaa ctgagaggat tctgtgatgg tcttgccatc caacatcgac
    42301 caatggacac cttccaagac attacggaaa ccgaggatca atccaccaac aatcaaagct
    42361 ggaataatcg gagtaaagat ttccgccaga gtggtcataa cacgttggac cacgttttga
    42421 ttactcttag ctgcagactt ggctgcttct ttagaaacac cctcaatacc tgaaacggct
    42481 gtaaaatcat tataaaagat gggcacgtca tttccaatga ttacctgaaa ttgacctgca
    42541 tttgtaaaag ttcctttaac agctggaatt gactcgatag ctttaacatt agccttctta
    42601 tcatctccta aaacaaagcg catccgtgtc gcacagtgag ttacggcagt cacattttct
    42661 ttgcctccaa ttgcctgaag cagatctttg gcttcttgtt caaattttcc catttgattc
    42721 tccttatact tgtcaaagca agtttgattt gataagtata ttgtaaacga taccaaactt
    42781 gtctgcaact tgtttggtat aaaaagggtg tttttttgta aattttctag cacttgtatg
    42841 gtaaaataga tacaggtgtt cattaaacta gactaaaaac ctatttaagc aggcaaaatg
    42901 aagaaatacc aacaattatt taagcaaatc caagaaacca ttcaaaacga gacttacgct
    42961 gtcggagatt tccttcctag cgagcacgac cttatggagc aatatcaagt gagtcgtgat
    43021 accgtccgaa aggccctgtc tctcctccaa gaggaaggat tgatcaaaaa gataagaggg
    43081 caaggttctc aagtcgtcaa agaagaaacc gtcaatttcc ctgtatccaa cctaaccagc
    43141 taccaagaac tagttaaaga acttggactg cgctctaaaa ccaacgtggt cagtctggac
    43201 aagattatta ttgataaaaa atcctcactg ataaccggtt tcccagagtt tcggatggtt
    43261 tggaaggtgg tccgccagcg tgtggtggat gatctggtat ccgttctgga tacagactat
    43321 ctggatatgg aactcatccc aaatctcact cgccaaattg ctgagcagtc tatctattct
    43381 tatatagaaa atggcctcaa actccttatt gattatgctc agaaggaaat caccattgac
    43441 cactcaagcg accgagacaa gattctcatg gacattggca aagaccctta tgtcgtttcg
    43501 attaaatcaa aagtctatct ccaagacgga cgccaatttc agtttaccga aagtcgccat
    43561 aagttagaga aatttagatt tgtagatttt gcaaaacgca agaaataaaa gactgagaca
    43621 ccagatctca gcctttttcg gctctataat atttgtagtg ggtaaccccc cctatagata
    43681 ttatggagcc tattttattg tagaaaaaaa gtcccataag atctataatg aaaagcgaca
    43741 aaacaactca ttagaaagaa tcatatggaa caattacatt ttatcacaaa attactagac
    43801 attaaa
//
LOCUS       DAVHIT010000005        42382 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVHIT010000005 DAVHIT010000000
VERSION     DAVHIT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.005"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..251)
                     /locus_tag="VVN16_000191"
     CDS             complement(<1..251)
                     /locus_tag="VVN16_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5571645.1"
                     /translation="MAKNSPQDLKNREYFSNNLNRVMKEKGIRQIDLHNALEIPKSTI
                     TGYVKGRSMPTSGNLQKLADYLGVKKSDLDPRFSDKDVY"
     gene            404..634
                     /locus_tag="VVN16_000192"
     CDS             404..634
                     /locus_tag="VVN16_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5571646.1"
                     /translation="MAQWTLRACRVNAGFTLRQVAKKVNKNFQTISKYEKDSTLIPFE
                     LLKELSELYQVKLDDIFLGDSTKKIELNQKEI"
     gene            650..1276
                     /locus_tag="VVN16_000193"
     CDS             650..1276
                     /locus_tag="VVN16_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU5571647.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIETWEAKPKHAHSTMNNLLLKAVTDMNAKQLREERGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSA"
     gene            1648..2100
                     /locus_tag="VVN16_000194"
     CDS             1648..2100
                     /locus_tag="VVN16_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571648.1"
                     /translation="MTETTYENLTRHIDRISAEMREIGEKNGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSHKIEAVSKSTTGMFFHAGIFEMDAMRQAFFKRQAMHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            2097..2297
                     /locus_tag="VVN16_000195"
     CDS             2097..2297
                     /locus_tag="VVN16_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571649.1"
                     /translation="MTLNLDNMTQAEFDEVMAEIKEKRPNLFQFIADFVDRKVSTEEV
                     DEYLKMKHEKQVEFIKNYQARA"
     gene            2299..2514
                     /locus_tag="VVN16_000196"
     CDS             2299..2514
                     /locus_tag="VVN16_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571650.1"
                     /translation="MNELDLTNTQSVIFMVVLIGLLLYLNHRDRKKSAQFERENQQRI
                     ETPSEDLSPDYGRYIQLAGVRVYGGTE"
     gene            2511..2852
                     /locus_tag="VVN16_000197"
     CDS             2511..2852
                     /locus_tag="VVN16_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5571651.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRHSVPIVGVRHGTFRGYFIPANKEELLDGAKAFYNQVQEEQKRLTVLLNADLESY
                     KETLKEVGGYV"
     gene            2845..3126
                     /locus_tag="VVN16_000198"
     CDS             2845..3126
                     /locus_tag="VVN16_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU5571652.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEARDTVKPRLTGLISAQEVM
                     DELDIKYMTLQKWEKAGLRRYQPPLEDTRKIYYKISDIWKFLGVENGKV"
     gene            3261..4127
                     /locus_tag="VVN16_000199"
     CDS             3261..4127
                     /locus_tag="VVN16_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEU5571653.1"
                     /translation="MTIYEAKGFQSNLVYPFDKIEPFQYIERFKPLVVPEGADIEEYK
                     RTQAPYCISGKVTPEKHGSYKRNNSSLIYRDLIFLDYDDIQGTSEGFIKAISSALFGY
                     SYILYPTIKHSLEKPRFRLVVKPDNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPASYQKIVEHGLDYPVPQTTAEPVKQNAASLPYTPRPSGQKSMTMRIIDT
                     LFNGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTF
                     SSIARAEYRKRG"
     gene            4180..5649
                     /locus_tag="VVN16_000200"
     CDS             4180..5649
                     /locus_tag="VVN16_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEU5571654.1"
                     /translation="MEQEHPPRTMKELENRIFKAGEEWRAEHTETKVNENTGDVTEKV
                     AMPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKTFDV
                     RIKPREWPQIKLMVRTLAKIRKPLESANLIPVQNGIINLETKELLPFSPKYVITSKIS
                     TAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAIDPNHTRNKFAIFYG
                     DGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYLKNP
                     SDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVPFNA
                     DFNGEKEKPWIKNEFLANKEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDNDYL
                     LSWVKHEYMEKGWHELDVVPVFIVTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQLTP
                     NRYSLRKKRVSPEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            6002..6169
                     /locus_tag="VVN16_000201"
     CDS             6002..6169
                     /locus_tag="VVN16_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571655.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEIDDFMATVEVLDVKFSGTPSSESF
                     NSVAIVLVLYR"
     gene            6253..6783
                     /locus_tag="VVN16_000202"
     CDS             6253..6783
                     /locus_tag="VVN16_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571656.1"
                     /translation="MTLKTFSSKAKTFTFTYEFKDLDTALVAGHALLGYMTGTYCQPV
                     ISLTYKDKGTLVAEYVEDHKLNKTFKRICDSFKDYHKQPGEAEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELELLDYADRLLSDKPIPMDSMTAFATLEMLGDESISLLQKLD
                     VEGEYKGLAGYTEHLK"
     gene            6854..7363
                     /locus_tag="VVN16_000203"
     CDS             6854..7363
                     /locus_tag="VVN16_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571657.1"
                     /translation="MKQLAIEAITKPMKLRGISKGIAELDGQRLEIDLDSLMIDFGGE
                     SFELDRIAGTKGGNRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKHTLNRSKTDCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDSFPERPKYMKQKRYYKHYQKFINYTRKGDSF
                     WLNGLSSLR"
     gene            8559..8978
                     /locus_tag="VVN16_000204"
     CDS             8559..8978
                     /locus_tag="VVN16_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU5571658.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELETLEGGIIKKPTLSHSR
                     VQTSRENKTENNLISVLKLKEDTLQRIERLTEERMKISELIDKLANPLERSVLRLFYL
                     NDLVVSEVAEEIDRSNTTVYIIRQKAIEHLTGIVNGD"
     gene            9467..12298
                     /gene="uvrA"
                     /locus_tag="VVN16_000205"
     CDS             9467..12298
                     /gene="uvrA"
                     /locus_tag="VVN16_000205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5571659.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            12291..13352
                     /locus_tag="VVN16_000206"
     CDS             12291..13352
                     /locus_tag="VVN16_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU5571660.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            13484..13882
                     /gene="spx"
                     /locus_tag="VVN16_000207"
     CDS             13484..13882
                     /gene="spx"
                     /locus_tag="VVN16_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU5571661.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            13948..14517
                     /locus_tag="VVN16_000208"
     CDS             13948..14517
                     /locus_tag="VVN16_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5571662.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGFENLLKNEPSKYISDRLANGATEQ"
     gene            14604..14870
                     /locus_tag="VVN16_000209"
     CDS             14604..14870
                     /locus_tag="VVN16_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU5571663.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            14874..15293
                     /gene="ruvX"
                     /locus_tag="VVN16_000210"
     CDS             14874..15293
                     /gene="ruvX"
                     /locus_tag="VVN16_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU5571664.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            15309..15614
                     /locus_tag="VVN16_000211"
     CDS             15309..15614
                     /locus_tag="VVN16_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU5571665.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            15922..17172
                     /locus_tag="VVN16_000212"
     CDS             15922..17172
                     /locus_tag="VVN16_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU5571666.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFIRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            17169..17711
                     /locus_tag="VVN16_000213"
     CDS             17169..17711
                     /locus_tag="VVN16_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU5571667.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            17857..19389
                     /gene="cls"
                     /locus_tag="VVN16_000214"
     CDS             17857..19389
                     /gene="cls"
                     /locus_tag="VVN16_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU5571668.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            19467..19610
                     /locus_tag="VVN16_000215"
     CDS             19467..19610
                     /locus_tag="VVN16_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571669.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            19647..21185
                     /locus_tag="VVN16_000216"
     CDS             19647..21185
                     /locus_tag="VVN16_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU5571670.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTNQGDQAGQLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVEVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            21305..23512
                     /gene="nrdD"
                     /locus_tag="VVN16_000217"
     CDS             21305..23512
                     /gene="nrdD"
                     /locus_tag="VVN16_000217"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU5571671.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            23524..23664
                     /locus_tag="VVN16_000218"
     CDS             23524..23664
                     /locus_tag="VVN16_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571672.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            23708..24214
                     /locus_tag="VVN16_000219"
     CDS             23708..24214
                     /locus_tag="VVN16_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5571673.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            24207..24797
                     /gene="nrdG"
                     /locus_tag="VVN16_000220"
     CDS             24207..24797
                     /gene="nrdG"
                     /locus_tag="VVN16_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU5571674.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGILYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            24794..25411
                     /locus_tag="VVN16_000221"
     CDS             24794..25411
                     /locus_tag="VVN16_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU5571675.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            25677..25985
                     /gene="rpsJ"
                     /locus_tag="VVN16_000222"
     CDS             25677..25985
                     /gene="rpsJ"
                     /locus_tag="VVN16_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU5571676.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            26202..26828
                     /gene="rplC"
                     /locus_tag="VVN16_000223"
     CDS             26202..26828
                     /gene="rplC"
                     /locus_tag="VVN16_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU5571677.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            26853..27476
                     /gene="rplD"
                     /locus_tag="VVN16_000224"
     CDS             26853..27476
                     /gene="rplD"
                     /locus_tag="VVN16_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU5571678.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            27476..27772
                     /locus_tag="VVN16_000225"
     CDS             27476..27772
                     /locus_tag="VVN16_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5571679.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            27790..28623
                     /gene="rplB"
                     /locus_tag="VVN16_000226"
     CDS             27790..28623
                     /gene="rplB"
                     /locus_tag="VVN16_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5571680.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            28727..29008
                     /gene="rpsS"
                     /locus_tag="VVN16_000227"
     CDS             28727..29008
                     /gene="rpsS"
                     /locus_tag="VVN16_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5571681.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            29020..29364
                     /gene="rplV"
                     /locus_tag="VVN16_000228"
     CDS             29020..29364
                     /gene="rplV"
                     /locus_tag="VVN16_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU5571682.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            29377..30030
                     /gene="rpsC"
                     /locus_tag="VVN16_000229"
     CDS             29377..30030
                     /gene="rpsC"
                     /locus_tag="VVN16_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU5571683.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            30034..30447
                     /gene="rplP"
                     /locus_tag="VVN16_000230"
     CDS             30034..30447
                     /gene="rplP"
                     /locus_tag="VVN16_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU5571684.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            30457..30663
                     /locus_tag="VVN16_000231"
     CDS             30457..30663
                     /locus_tag="VVN16_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU5571685.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            30688..30948
                     /gene="rpsQ"
                     /locus_tag="VVN16_000232"
     CDS             30688..30948
                     /gene="rpsQ"
                     /locus_tag="VVN16_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5571686.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            30974..31342
                     /gene="rplN"
                     /locus_tag="VVN16_000233"
     CDS             30974..31342
                     /gene="rplN"
                     /locus_tag="VVN16_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5571687.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            31420..31725
                     /gene="rplX"
                     /locus_tag="VVN16_000234"
     CDS             31420..31725
                     /gene="rplX"
                     /locus_tag="VVN16_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5571688.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            31749..32291
                     /gene="rplE"
                     /locus_tag="VVN16_000235"
     CDS             31749..32291
                     /gene="rplE"
                     /locus_tag="VVN16_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5571689.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            32309..32578
                     /gene="rpsN"
                     /locus_tag="VVN16_000236"
     CDS             32309..32578
                     /gene="rpsN"
                     /locus_tag="VVN16_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU5571690.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            32863..33261
                     /gene="rpsH"
                     /locus_tag="VVN16_000237"
     CDS             32863..33261
                     /gene="rpsH"
                     /locus_tag="VVN16_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5571691.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            33453..33989
                     /gene="rplF"
                     /locus_tag="VVN16_000238"
     CDS             33453..33989
                     /gene="rplF"
                     /locus_tag="VVN16_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU5571692.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            34073..34429
                     /gene="rplR"
                     /locus_tag="VVN16_000239"
     CDS             34073..34429
                     /gene="rplR"
                     /locus_tag="VVN16_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU5571693.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            34447..34941
                     /gene="rpsE"
                     /locus_tag="VVN16_000240"
     CDS             34447..34941
                     /gene="rpsE"
                     /locus_tag="VVN16_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU5571694.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            34913..35137
                     /gene="rpmD"
                     /locus_tag="VVN16_000241"
     CDS             34913..35137
                     /gene="rpmD"
                     /locus_tag="VVN16_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU5571695.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            35282..35722
                     /gene="rplO"
                     /locus_tag="VVN16_000242"
     CDS             35282..35722
                     /gene="rplO"
                     /locus_tag="VVN16_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU5571696.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            35735..37045
                     /gene="secY"
                     /locus_tag="VVN16_000243"
     CDS             35735..37045
                     /gene="secY"
                     /locus_tag="VVN16_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU5571697.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            37196..37834
                     /locus_tag="VVN16_000244"
     CDS             37196..37834
                     /locus_tag="VVN16_000244"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU5571698.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            37951..38169
                     /gene="infA"
                     /locus_tag="VVN16_000245"
     CDS             37951..38169
                     /gene="infA"
                     /locus_tag="VVN16_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5571699.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            38194..38310
                     /gene="rpmJ"
                     /locus_tag="VVN16_000246"
     CDS             38194..38310
                     /gene="rpmJ"
                     /locus_tag="VVN16_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU5571700.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            38328..38693
                     /gene="rpsM"
                     /locus_tag="VVN16_000247"
     CDS             38328..38693
                     /gene="rpsM"
                     /locus_tag="VVN16_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU5571701.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            38711..39094
                     /gene="rpsK"
                     /locus_tag="VVN16_000248"
     CDS             38711..39094
                     /gene="rpsK"
                     /locus_tag="VVN16_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5571702.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39137..40072
                     /locus_tag="VVN16_000249"
     CDS             39137..40072
                     /locus_tag="VVN16_000249"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5571703.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            40084..40470
                     /gene="rplQ"
                     /locus_tag="VVN16_000250"
     CDS             40084..40470
                     /gene="rplQ"
                     /locus_tag="VVN16_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5571704.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            40735..41001
                     /locus_tag="VVN16_000251"
     CDS             40735..41001
                     /locus_tag="VVN16_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5571705.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            41011..42348
                     /locus_tag="VVN16_000252"
     CDS             41011..42348
                     /locus_tag="VVN16_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU5571706.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        13454 a         7874 c         9470 g        11584 t
ORIGIN      
        1 ttgtaaacat ctttgtcaga aaagcgaggg tcaagatcag attttttcac tcccaaataa
       61 tcagcaagtt tttgaagatt gccagatgtt ggcattgatc taccttttac ataaccagtt
      121 attgtacttt taggaatttc taaagcatta tgcaggtcta tctgtctgat acctttttct
      181 ttcataacac ggttaagatt attagagaaa tattctctat tctttaaatc ctgagggcta
      241 ttttttgcca ttgttaaaaa ctcccttact ttaattctac aacctgatta taacacaaaa
      301 aagacaaagt acgaaaaaaa taaaattttt caacaacagc gttgacaaag tacgaaaaaa
      361 atagtaccat gattttatca atcttgaaag gagctataag aatatggcac aatggacgct
      421 aagagcgtgt cgagtaaatg caggttttac tttgcgccaa gtggctaaga aggtcaacaa
      481 gaattttcag actatctcaa agtacgaaaa agatagtacg ctaatccctt ttgagctact
      541 taaagagtta tcggaactgt atcaggtaaa actggacgat atttttttag gagatagtac
      601 gaaaaaaata gaactaaatc aaaaggaaat ctaagaaagg agcaaaccaa tggaattagt
      661 ctacatggac ggtaagaaag agccgtatac actgagcagt attgttgcgg agtgtgcaga
      721 agttaagcac agacatttga agattttgct gaataaacat cgagaggact tcgagagttt
      781 cggaaaggtg caatttaaaa tttcaccttc agaaagtggg caaaatgtac gggactatat
      841 tttgaatgag caacaagcaa ccttgctgat cacttactta cgaaatacag aacccgtaaa
      901 agagtttaag aagaacctgg tcaaagcatt ctttgaaatg cgtgaggagc tttctaagtt
      961 ccgtatgcag agggcgctag aaaagccaaa aagaaaaacc ttgcatgaca gtatagagac
     1021 atgggaggca aaacccaagc acgcgcatag caccatgaac aacctgctac taaaagcagt
     1081 aactgacatg aacgctaagc agttaaggga agaacgtggg ggctataacg gtattgatag
     1141 cttgaccagt gacgagctgg agcaatacca ggcatttgag gatatggtaa tagccatgat
     1201 cggcttgaat atgagttacc aagaaatcaa ggctatggca ttcagaaata aaaaaacacg
     1261 ccaaagaagc gcgtgagagc aacaaaaaag gcttagcaat aaccacaccg cagagccttt
     1321 cacactaaca ctaaaacgaa tttaacaaag caggcaagct attatcaaag aggttttagt
     1381 aaagatttat acctagatta tagcatattt agggcaattt gaccatacgg agagcgccaa
     1441 ctctttaaac tggtactttc tcatgctctc aatttggcga ctcagagcgt gaggctatga
     1501 attgcaagaa aaactgtata agaaacaacc attaaaaacc cccacactct cggtcggcaa
     1561 acttctgagc gtgaggatat ccagtatagt aaaaggcatt aaaagctctt agagcaatta
     1621 cacaacatag aaacaaaggt aaaaaacatg acagaaacaa catacgagaa tttaactaga
     1681 catatagaca gaattagcgc agaaatgcga gaaattggcg aaaaaaatgg gcttagtaga
     1741 ctttcattat tggcaaatca gacaaaattc atcaaagaaa atttacaccg tttactttgg
     1801 attgaacttc ctgaattgaa tgacagccat aaaatcgagg cagtttctaa aagcactacg
     1861 ggaatgtttt tccacgctgg tatttttgaa atggacgcta tgcgacaagc attctttaaa
     1921 cgccaagcta tgcacttttt tgacaacgaa gcagagcaac aggcgtatat agaacatgct
     1981 gaaaaggagt atttagaggc tactataacc ttaaaagata ttctttttaa ctctaaaaat
     2041 ggaactcaga gagtaaataa aagttgtctt atagagaagt ttgaggaggc aatgcaatga
     2101 cactaaacct agacaacatg acacaagcag agtttgatga agtaatggct gaaattaagg
     2161 aaaaacgccc caacctcttc cagtttattg ctgattttgt agatcgaaaa gtaagcactg
     2221 aggaggtaga cgaatacctg aagatgaaac atgaaaaaca agtggaattt atcaagaatt
     2281 atcaagcgag ggcataacat gaatgagtta gatttgacca atacacaatc ggtaatcttt
     2341 atggtggtat tgattggctt actgctttac ctaaaccacc gagaccgcaa aaaaagcgcc
     2401 caatttgagc gagaaaacca acagaggata gaaacaccta gcgaggattt aagccctgat
     2461 tatgggcgtt atatccagct tgcaggggta agagtttacg gaggaactga atgagtctat
     2521 cagagaatga caaacgagta ttaagactaa tcaaggtagg ggctgagaac tccataacag
     2581 gggcagaaat cagccttata actaagctgg cggaaagaac cgtacaagat attatcagcc
     2641 gtctaatcac gcgtcatagt gttcctattg ttggggttcg ccatgggaca tttagaggat
     2701 attttatccc agctaacaaa gaggagctac tggacggagc aaaggcattc tataaccaag
     2761 tacaggaaga acagaaacgc ctaactgtcc ttttgaatgc tgatttagag agttacaaag
     2821 aaaccttgaa ggaggtgggc ggatatgttt agcctaagcc gagaaagtga gcaagaccta
     2881 gcgcatggca ttctggaggt agtagaaaga tacctggaag cgcgtgatac agtcaagcca
     2941 cgactgacag gtttaatatc agcccaggaa gtcatggacg agttagatat aaaatacatg
     3001 acactccaaa agtgggaaaa agcagggcta agacggtatc agccaccgct agaagatact
     3061 aggaaaatct attataaaat atctgacatc tggaagtttt tgggggtgga aaatggcaag
     3121 gtatagtatt catccggcag ataataaagg gcattatcac aatattaaat tttacaaaga
     3181 gcgcaggcca accctagacc agctaaagga acagcaaagg cttaaaaagc tgaagaaaaa
     3241 acgcaaaaag gggcgatagt atgacaattt atgaggctaa aggctttcag agtaatcttg
     3301 tctatccatt tgataaaatt gagcctttcc agtatattga acgattcaag cccttagtag
     3361 ttcctgaagg ggcagatatt gaggaataca agcgaactca agcgccttac tgtataagtg
     3421 gcaaagtgac gccagaaaag catggcagtt acaagcgaaa caactccagt ctaatctatc
     3481 gggatttgat tttccttgat tatgacgata tacaggggac gtctgagggc tttataaaag
     3541 ccatttctag cgctttattt ggctactctt acatcttata cccgactatc aaacatagct
     3601 tagaaaagcc ccgttttcgc cttgtagtga agcctgacaa cgtgatgaat gaggcgacct
     3661 ataagcaggt agtgaaagag attgctgaca agattggact cccctttgat atggccagtt
     3721 taacatggtc acaactccaa gggctaccag tcacaacagg cgacccagca agttatcaaa
     3781 agattgtaga gcatggactt gattatccag taccacagac aacggctgaa ccagtcaaac
     3841 agaatgcggc aagccttcca tacactccaa ggccaagcgg gcagaaatct atgaccatga
     3901 ggatcattga cacgcttttc aacggatttg gagacgaggg agggcgcaac gtagcactga
     3961 cacgatttgt aggcttactc tttaataagt gggttgattg tgacctagag acagcctatg
     4021 aacttacaaa gatagccaat agcgtgacag ttgaaccatt gccgattgag gagctagacc
     4081 gcacttttag cagtatagca cgggctgaat atagaaagag aggttagaac catagaacta
     4141 gaagaattag caaacctaaa aagtgaaatc ttggaggtaa tggagcaaga acaccctcct
     4201 agaacgatga aggagcttga aaatcgtatc tttaaagctg gagaagaatg gcgggcagaa
     4261 catactgaaa ctaaggtaaa tgagaacaca ggggacgtta ccgaaaaggt tgccatgccc
     4321 caaaccttca cagtagctaa aatactgagc gagattgtca catttacttt tatcagcaag
     4381 agcaatatac ctgattatag cctactctat atctatgatt tagacgaggg tatctatacc
     4441 gctagtaatg atctattcaa tcttttgtgt aagacctttg acgttagaat taaacccaga
     4501 gagtggccac agattaagtt aatggttaga acattggcaa agatacgaaa acctttagaa
     4561 agtgctaacc tcattcccgt acagaacggc attatcaact tagaaactaa ggaactactc
     4621 ccctttagtc ctaagtatgt aattacaagt aagataagca cggcctacca cgcgcctaaa
     4681 cgagtcccaa cagatagaga gggaaaaact tttgatgatt ggttgaactc aatcgcttgt
     4741 aatgatagtg aactggtaac actgttttgg cagattatcc ttgaagctat tgaccctaac
     4801 catactcgaa ataagtttgc tatcttctac ggggacggta acaatggtaa agggacattt
     4861 cagcgcttcc ttatcaacct gataggggaa agcaatatat cagccttaaa gcctgcacag
     4921 tttgctgaaa agcataactt ggagaccttg gtaggtaagg tttgtaatat tggggatgaa
     4981 gcacctaacg aatacttaaa aaatccgtct gatttaatga gtatcactag tggcgacact
     5041 gttctagtca atccaaaagg acgcccagcc tttgaagcta ctttcaagtt attcaatatc
     5101 ttttcaggga attatatccc caatggtggc aacaagacaa aaggctggta tcgtagaatt
     5161 atgattgtcc cttttaatgc tgactttaac ggggagaagg aaaaaccttg gataaaaaat
     5221 gagtttttag caaacaagga agtcctagaa tatgccctct acaaagctat caatcaagag
     5281 ccatttactc actttatcga acctaaagca gttaaaggac tcctagaaga ataccaggaa
     5341 gataatgact acttactttc atgggttaag catgaataca tggaaaaagg ttggcatgag
     5401 ctggacgtag tacctgtttt tatcgttaca agatcactaa agcactatgc cgaagatatg
     5461 ggaataccca agcccaatgt ttacggggct ggtaaagaaa ctatcaagca cttacaacag
     5521 ttgacaccta atcggtattc tctaagaaag aaaagagttt ctccagaaga ttttgaaaaa
     5581 ctagaggcat gggacgttga caagataaaa atgcggacac ctcaacactc tatcactaaa
     5641 caagaataag tgtgacgttc tttttttgct taaacccttg ataatactga ttttcttgaa
     5701 aaaagtgtga cgttgtgtga cgttctcaaa aaagaaggtc acaccccttg aagccttgat
     5761 atatctaact ttaggggaat aggtgtgacg ttgtgacatt ctttccaact cttatatata
     5821 gagaaaaata cagtatttat atatatagga acgataaaac aaaagaacgt cacagggtca
     5881 caggggtggt gaaacaccta gagcctcaag ggtttgaacg tgtgacgttc tttgtcacaa
     5941 aaggtcacag aaaacgaaaa atattacata atttacctta aaacaaagaa atgacaacaa
     6001 catgaaagtt aaactatttg aatatactag acgatgggaa aaagaagact ttgagaaaga
     6061 gatcgatgac ttcatggcaa ctgttgaagt gctggacgtt aaattttctg gtacaccatc
     6121 tagtgaatcc tttaattcgg tggctatcgt attggtatta tacagataac aaaatggggt
     6181 agatagtacc gacccccttg caatggtgta actactagtg acacccttaa aaacagaaag
     6241 aggaaaaaca acatgacatt aaaaacattt tcatctaaag ccaaaacatt tactttcact
     6301 tacgaattta aagacctaga tacagctctg gtagcaggtc acgcgttact gggatatatg
     6361 actggaacat attgccagcc tgtaatctca ttgacctaca aggataaagg cacgctagta
     6421 gctgagtatg tggaagatca caagctaaac aagaccttta agcgtatttg tgacagtttt
     6481 aaagactacc acaaacaacc aggagaggct gaagcatttg aagaacgtta caaacgggag
     6541 cgcgtgctcc aactcaaaga gtcagaggac tttgagagtt tactgaataa ggtcacagac
     6601 tacgagctgg aactattaga ctatgctgac cgcctgcttt ctgataagcc tatccctatg
     6661 gactctatga ctgcttttgc tacgttggaa atgctgggcg atgaaagtat tagcctactc
     6721 caaaagttgg acgtagaggg cgaatataaa ggccttgctg gttatacaga gcatttgaag
     6781 tagaatacag gggagcaatc ccctttttgc tatcaagtca atagttttgg gtttttccat
     6841 atagggggaa ataatgaagc agttagcaat agaagcaatc actaaaccaa tgaagctgag
     6901 aggaatatct aagggtatag ccgagttgga tggtcaacgt ttagagattg atttggatag
     6961 tttaatgatt gattttggtg gagagtcatt tgaattagac agaatagcag gaactaaggg
     7021 aggtaatcga tatttttttc tttgccctga ttgtggtaga cggtgcaggt tgctttataa
     7081 acggtatttg tattttagtt gcggtacttg tctagatatc cacaagcaca ccctgaaccg
     7141 cagtaaaacg gattgccaat actactggga gcttgcacta aaagaagcta gaaaggtaga
     7201 accagggtgg agtcctagac gtggcggata tatgtttgat agttttcctg aaagaccaaa
     7261 atacatgaaa cagaaacgat attacaagca ttatcaaaag tttattaact acaccagaaa
     7321 aggggatagc ttttggctga atggtttgag ttctctaagg taatttgtag tagtgtgcaa
     7381 tttacaaaac aaacaaatac tacatattag acggtttgaa tgttaaaaaa gctagatata
     7441 tcaaggattt aattaagtat agcgttccaa aaaaagggtg cagtaagcgt tccaaaaaag
     7501 ggtgaggttg aatatttaaa aaaggctaag attaggctaa ggtcacatac ttcaaaatag
     7561 caaggtttta gcaaggtaca gggctttttt ctagtgatag aaccccaagg ttaccacaag
     7621 gtatagctac ttttttagaa ccatagaacg gtaaggttac ggtaaggtat cgaagttttc
     7681 caatggtaag gttttggtaa ggtagcatat ataaaaagta caaggttagt acaaggttac
     7741 atacttaaaa atactaaggt tttacaaagg tagagaggtt taccgagggt tagaaaaggt
     7801 gagattttgc ttaggtagtg gcagtaaata tgcctgacaa atggcagggt tatgtgtgtt
     7861 gaactccgag cgaacgccga gaaagtggct atatcccccc tatcatttat caagtagata
     7921 agagcaataa aaaccctcct taaccccctg agaaacgaca agcagataag agaagttttt
     7981 agccctctta tttcggatct actccttgca gaatgacaag cagataagga cgacaataag
     8041 ccttcttaac tgcttacaaa acgataggga gttaagacga atttaaaagc cccttatttt
     8101 cgtttttctc cttgtcaaac gggaaggaaa taagacagga aataacatcg cttattttga
     8161 ttttgctccc tgaaaaactg taaggagata aggagactta ttagcttcct taatttgaat
     8221 tttctcctta caaaatgaca gggaaataag aactaaaata actcccctta ttttcctacc
     8281 aaatgataag gagataagag cagtttaaaa gtctgttatt ttggatttgt tgcttacaaa
     8341 atgataagca gataagcaaa gaaataactt gccttaattg cttgcagaat gacaaggaaa
     8401 taagagagat aaaaacatcc cttaattgct tatcaaatgg tatggagaat ggacttgaac
     8461 ctagagcgaa gctagagaaa atgaaaaaaa gccagcacag ggctgactcc tttatgaatt
     8521 attccataaa actattatat cacaaaggag gccgaggcat gacaccagag caagtaaaag
     8581 aaaaactaga gggcgttaag tggataaaca aggagatcaa aggcttatat ttggaattgg
     8641 aaaccctgga aggtggcatt atcaaaaaac caacactaag ccatagcagg gtgcagacaa
     8701 gtagagagaa taagacagag aacaatctta taagtgttct gaagctaaaa gaggacactt
     8761 tacagagaat tgagcgactt actgaagaga gaatgaaaat atctgagcta atcgataaac
     8821 tggccaatcc gcttgagcgt tctgttctaa gactttttta cttgaatgat cttgtagttt
     8881 cggaggtggc tgaagaaata gatagatcaa atactacagt atacattata aggcaaaaag
     8941 ctatagaaca cttaacaggt attgtaaacg gggattgatt taaccttacc acggaattag
     9001 gagagtatgg gggagtccta agccacatat tcgcccgtat ttcgctttgt ttcgtactgg
     9061 acaactaaaa caccatggat aaactacaaa ccctcagaaa cgaatctggg ggcttttgtg
     9121 aggtcataat cttataaacc acgcgcttca tggtataata tatctatcag caaccagcaa
     9181 taaaaaagca cgtttgaccg tgctagtttc ttgcctgctg aactcgtcaa atcttagccc
     9241 ttttgtgggg cttttttgct cccctttttg cgtactttgg gggagcaagt agtaaaaagt
     9301 aatgttagta atttttatta aaaagcgcgt gatataaggg ttaggaagcc ctaaggaaac
     9361 ataaaataag cgattgttga ttatactaca aaatcggcct tttgggtaat agaatctcac
     9421 tttttttggt ataatggtaa gcaataatgg actagaaaga acaaagatgc aagataaaat
     9481 tgtcattcat ggggcgcgtg cccataattt aaaaaatatt gatgtggaga ttccgcgaga
     9541 caagttggtt gtcgtgactg gcttgtcagg ttcagggaag tccagtctgg cttttgatac
     9601 tctctatgcg gagggacaac gtcgctatgt agagagtttg tctgcctacg ctcgtcagtt
     9661 cttgggaaat atggaaaaac cagatgtaga tgccattgat ggtctcagcc cagctatttc
     9721 catcgaccag aaaacgacta gtaaaaatcc tcgctcgacg gtgggaacga cgactgaaat
     9781 caatgactat ctgcgtctcc tctacgcacg tgtggggacg ccttactgta tcaacggaca
     9841 tggagctatc aatgcttctt ctgtggagca aatcgttgat aaggttttgg agttacctga
     9901 acgccagcgc ttgcagatct tggctcctgt catccgcaag aaaaaaggcc aacataagag
     9961 tgttatcgag aaggttcaga aagacgggta tgttcgtgtt cgtgtggatg gggaagtcta
    10021 tgatgtgacc gaagtgccag agttgtctaa gagcaagcaa cacaatatcg atgtcgtggt
    10081 tgaccgtatt gttatcaagg agggcattcg tagccgtctc tttgattcca ttgaagctgc
    10141 ccttcgtatc gcagaaggtt atgtgattat cgacaccatg gacgactctg agttgttgtt
    10201 ctctgagcat tatgcctgtc cagtttgtgg atttactgtc ccagagttag agcctcgtct
    10261 cttctctttc aatgctccct ttggctcttg tagtgagtgt gacggcttgg ggattaagct
    10321 ggaggtggat actgatttgg tagtgccaga tgccagcaaa accttacatg agggagcgct
    10381 ggcaccttgg aatcctatct catccaacta ctatccaaac atgttagagc aggccatgaa
    10441 agtctttgga gtggctatgg ataagccttt tgaggacctg tcagaagaag ataagaactt
    10501 gattctctac ggctcagatg gtaaggaatt ccatttccat tatgaaaatg aatttggtgg
    10561 tgtgcgcgat atcgacattc cgtttgaggg agttatcaat aatatcaaac gtcgttacca
    10621 tgaaaccaat agcgattaca ctcgcactca gatgcgtctc tacatgaatg agctgacctg
    10681 cggaacctgt caaggctatc gtctcaatga ccaggccttg tctgtccgtg tgggcggcca
    10741 gcaagggcca catattggag aaatctcaga cctgtctatc gctgaccact tggacttggt
    10801 gagccagtta actttgtctg aaaatgaagc catcattgct cgtcccattc tcaaggaaat
    10861 caaggatcgt ttgaccttcc ttaataatgt gggtcttaac tatctaaccc tgtcacgttc
    10921 agcaggaacc ctttcaggtg gggaaagtca gcgcattcgt ttggcaaccc agattggttc
    10981 caacctatca ggtgtcctct atatcctaga cgagccgtca atcggtcttc accagaggga
    11041 caatgaccgc ctgattgcca gtctgaaaaa gatgcgtgac ttgggcaata ctcttatcgt
    11101 ggtggaacac gacgaagata ccatgcgtga agctgattat ctgattgacg ttggtcctgg
    11161 tgccggtgtt tttggtggtg agattgttgc agcaggtaca cctaagcaag tggctcgcaa
    11221 cagcaagtct atcacaggtc agtacttgtc aggcaaacgt gtcatcccag taccagaaga
    11281 gcgccgtgtc ggaaatggtc gttttattga agtgacagga gcgcgtgaga acaacttgca
    11341 aaatgtcacg gctcgctttc cactaggaaa atttatcgca gtgacaggtg tgtcgggttc
    11401 agggaaatcg actctaatca acagcattct caaaaaagcc attgcccaga agctcaaccg
    11461 taattcagac aaacctggta agtttaagac tattacaggg attgagcatg tcgaccgttt
    11521 gattgatatt gaccagagcc ctatcggacg gacgccgagg tctaacccag ctacctatac
    11581 aggagttttt gacgatatac gtgacctttt tgctcagaca aatgaagcca agattcgagg
    11641 ctacaagaag gggcgcttca gtttcaacgt caagggaggt cgctgtgaag cttgctcagg
    11701 tgacgggatt atcaagattg agatgcactt cttgccggat gtttatgtgg cttgtgaagt
    11761 ttgccacgga acccgctata acagtgaaac cctagaagtt cactacaagg aaaagaatat
    11821 ttcgcaggtc ttggatatga cggtcaacga tgcggtagaa tttttccaac acattccgaa
    11881 aattcaacgc aaactccaga ccatcaagga tgtaggtcta ggctatgtga ccttgggaca
    11941 gccagctacc accctttctg ggggagaagc ccaacgtatg aagttagcta gcgaactcca
    12001 caaacgctcg acaggaaaat ctttctacat tctggatgag ccgacgacag ggcttcatac
    12061 agaggacatt gctcgcctgc tcaaggtttt agctcgcttt gtagacgacg gcaatacagt
    12121 cctcgtcatc gagcacaatc tggatgttat caagacggca gaccatatca ttgacttggg
    12181 acctgagggc ggtgtcggtg gtggaaccat catcgtaaca ggaactccag aagaagtagc
    12241 tgccaacgaa gccagctata caggacacta tttgaaagga aagttacatc atgaataaac
    12301 gcgtacaagc atttctagct aaaatgcaag aaaaagaact agatggtatc atcatcaaca
    12361 atcttaaaaa cgtctattat ttgactggtt tttggggctc aaacggaaca gtctttatca
    12421 gccgtgaccg tcaggtctta gtgacagact ctcgctatat catcgcagct aagcaagaaa
    12481 ccagtggttt tgagattgtg gctgatcgtg atgaattggc tgtcattgca ggaattgtta
    12541 aggacatggg cttgactcgt atcggttttg aagatgagat ttcagtgtct tattaccacc
    12601 gtatgcaggc agcttttgca ggtttggact tgcttccaca aactcagttt gtggaaggtc
    12661 ttcgtatgat taaggatgag gcagagattg cagcgattcg caaggcttgt tctatctcag
    12721 accaagcttt ccgcgatgcg cttgacttta tcaaaccagg aaaaactgaa attgagattg
    12781 ccaacttcct tgatttccgc atgcgtgagt tgggagcatc tggcttatct tttgatacga
    12841 tcctagctag cggtatcaat tcttctaaac cccatgccca tccaatgcac aaaccagtgg
    12901 agttgggaga agccattacc atggacttcg gctgtctcta tgaccactat gtcagtgata
    12961 tgacccggac tatctatcta gggcatgtta gcgatgagca ggcagagatt tacaatacgg
    13021 ttctaaaagc taaccaagcc ttgattgacc aagctaaggc aggcttaggt ttccgtgact
    13081 ttgacaaaat ccctcgtgat attatcattg aggcaggtta tggtgactac tttactcacg
    13141 gcattggcca cggtattggt ctggatatcc atgaggaacc ctactttagc cagacttcta
    13201 cagaaactat taagacaggt atggccttga ccgatgaacc aggtatctat atcgaaggca
    13261 aatatggcgt tcgtatcgag gatgatatcc tgattacaga gacaggttgt gaattattga
    13321 ccctagctcc aaaagagttg atagtcattt agttgtttgt caagaaaaaa gtacgaaaat
    13381 gacgaaaaaa gtcaaaaaaa attaaaaata ggtcgcaagt cggatgtttt ttatggtata
    13441 atagactaaa ctgatagtaa catgtagcga aaggggtagg tacatgatta aaatttatac
    13501 agtctcaagt tgtactagct gtaaaaaagc aaaaacctgg ctcaatgccc accagttaag
    13561 ttataaagaa caaaaccttg gtaaagaagg aattacgaga gaagaattac tggatattct
    13621 aaccaaaaca gataacggaa tagccagcat cgtttcgtct aaaaatcgct atgctaaagc
    13681 ccttggagtg gatattgaag atttgagtgt caatgaagtt ctcaatctga ttatggaaac
    13741 accgagaatt ttaaagagcc caatccttgt agatgaaaaa cgcctgcaag ttggctacaa
    13801 ggaagacgat attcgtgcct tcctaccacg ctctgtccgt aatgtagaaa atgcagaagc
    13861 acgtttgcgt gcagctctat aaacatcaag gctgggagca cttcccagtc ttattctatt
    13921 ttaatttcaa aaagaaagaa gaaagaaatg aaaaaaatag ttcttgttag tctagctttc
    13981 ctttttgtcc tggttggttg cggacagaaa aaagaaactg gaccagctac aaaaacagaa
    14041 aaagatacgc ttcagtcggc attgccagtt attgaaaatg ctgagaagaa tacagttgta
    14101 actaagactt tggtcttgcc caagtcagat gatggtagcc agcagacaca aacaattact
    14161 tacaaagaca agactttttt gagcttaacg attcaacaaa aacgtccagt ctctgatgag
    14221 ttgaagactt atattgacca acatggagtg gaagaaactc aaaaagctct tcttgaagcg
    14281 gaggagaagg ataagtctat cattgaagct cgtaaattgg caggtttcaa acttgaaaca
    14341 aaactattga gcgcaacgga acttcaaaca acgactagtt ttgattttca agttctggat
    14401 gtcaagaagg cttcccagtt ggaacatctg aagaatattg gttttgaaaa tcttttgaaa
    14461 aatgaaccaa gcaaatatat ttcagataga ttggcaaatg gcgcgacaga acaatagaaa
    14521 atccaaataa atagactgcc tgaattgtag aaggtaagga attatgctat aatggtacta
    14581 ttctaaggaa agagggtgtt agaatgggat ttactgaaga aacagtacgt tttaaattgg
    14641 acgattccaa taaaaaagaa attagcgaaa ctttgacaga tgtttatgct tcgttgaacg
    14701 ataagggtta caacccaatt aaccaaatcg taggttacgt attgagtgga gaccctgcct
    14761 acgttcctcg ttataataat gcacgaaatc aaatccgtaa gtatgagcgt gatgaaatcg
    14821 ttgaggaatt ggttcgttac tacctcaaag gacaaggagt cgatctataa cctatgagaa
    14881 ttatgggatt ggacgtcggt tcaaaaacgg taggggtggc gattagcgat ccgcttggtt
    14941 ttacagctca agggcttgaa atcatccaga taaatgaaga acaaggccaa tttggttttg
    15001 accgcgttaa ggaattggtt gatacttaca aggtggaacg atttgtagtg ggcttgccta
    15061 aaaacatgaa caatacaagt ggaccgcgcg tggaagctag tcaagcatac ggagcaaagc
    15121 tagaagagtt ttttggttta ccagtagact atcaggatga acgcttgaca acagtggctg
    15181 ctgagcgcat gttgattgaa caagcagata tcagtcgcaa taagcgcaag aaagtcattg
    15241 ataagttagc agctcagctg attttacaaa attatttaga tagaaaattt taatataaag
    15301 gagaggctat gtcacacgat cataaccacg accacgaaga acgtgaacta atcacactag
    15361 tagatgagca aggaaatgaa accttgtttg aaatcctttt gacaattgat ggaaaagaag
    15421 aatttggtaa aaactatgtt cttctagtac cagttaacgc agaagaagac gaagacggac
    15481 aagttgaaat ccaagcttac tcattcatcg aaaacgaaga tggaacagaa ggcgaattgc
    15541 aaccaatccc agaagactca gaagacgaat ggaacatgat tgaagaagtc ttcaacagct
    15601 ttatggagga gtgatacagt ctgggagact gtatcagccc aactcccaga aatggggaag
    15661 agttggaaca tccagtggat gtttttagcc cagttccttt aaataagaga gaactggtag
    15721 gttcggggga cgtttttagc ctgacgctaa aaataaaaac cgtcagtaag tccggggggc
    15781 gtttttagcc cacgttcaca ttcatagtag ctagttatta gctaaccagg taagaggtcg
    15841 ggacgaaaat cctggccttg tttttattag taactatact ttaatgcgat aattgattgg
    15901 acttttgtca aggagatgtg tatgtttgaa gtagaagaat ggcttcatag tcggattggt
    15961 ttgaattttc gatcaggttt gggccgaata cagcaagcgg tggatttgtt aggaaatcct
    16021 gagcagtctt accctattat ccacgtaaca gggactaatg ggaaaggatc taccattgct
    16081 tttattaggg aattatttat ggggcatggc aaaaaagttg cgacctttac ctcccctcat
    16141 atcgtatcca tcaatgaccg aatttgcatt aatgggcaac ctatagcaga cgcagacttt
    16201 atccgtttgg ctgatcaggt caaggagatg gagaaaacgc ttctgcaaac tcctgaccag
    16261 ttgtcctttt ttgaattgct gaccttggtt gcttttcttt attttaggga gcaggaggtg
    16321 gatttggttt tattagaagt gggaattggt ggcttacttg acacgaccaa tgtggtaact
    16381 ggagagcttg ctgtcatcac ctccattggg cttgaccatc aagaaacctt gggtgatagt
    16441 ctagaagcaa ttgcagagca gaaagctggt attttcaagg ctggtaaaaa ggcagtgatt
    16501 gcgaaattgc ctccagaagc tagacttgtc tgtcagaaaa aagccgaatc tttagctgtt
    16561 gacctttatc aggcaggtca agatttttta atgctgaatg gtgatttttc aagctcttta
    16621 ctaaatcttt cacagctgaa cataggctta gaaggagtct atcagcagga gaatgcagcc
    16681 ttggcgttgc aaacttttct tctttttatg ggagaaggaa aggaagctgt tgatgaacag
    16741 gctgtaagaa aggccttgga acagacccat tgggctggtc gcttggagcg tattcgccca
    16801 cagatttact tggatggtgc tcataacctc cctgccttga ctcgcttggt tgagtttatc
    16861 aaagaaaaag agcaggaagg ctatcgacct caaatccttt ttggagcctt gaaacggaag
    16921 gattatcaag ggatgttagg ttacctgact gaaaaattgc ctcaggtgga actcaaggtg
    16981 accggctttg actatcaggg ggctttggac gaaagggatg tgacaggtta cgatatagtt
    17041 tcttcttacc gagaatttat cagcgatttt gaagaaagag cagatgctca ggatttgctg
    17101 ttcgttacag ggtctctcta ttttatctca gaagtacggg gctacctgct ggaccgtgag
    17161 cagataaatt gacccccttg tttgaacttg ttatactagg ggaattgctg gttagagaaa
    17221 atttctctaa attggtagca gaaaggaaat tcatcatgaa attaaaaaga ttcacacttt
    17281 ctcttgcttc tctagcaagt tttagtctct tagtagcttg ttcacaaaga gctcaacagg
    17341 ttcaacagcc tgttgctcag caggtccaac agcctgctca acagaatacc aatactgcaa
    17401 atgcaggagg taaccaaaat caagcggctc cagtacaaaa ccaacctgtt gctcaaccga
    17461 ccgatattga tgggacttat actggtcagg atgacggaga ccgtatcact ttagtggtaa
    17521 ctggaacgac tggtacatgg actgagctcg aatctgacgg ggatcagaaa gtcaaacagg
    17581 ttacatttga ttcagcaaat caacgcatga ttattggcga tgatgtcaaa atttacactg
    17641 taaacggtaa tcaaatcgtc gtagatgata tggatagaga cccatcggac caaatcgttt
    17701 taactaaata agactatgca agtagaaagg gctggatttt tcagcctttt tacttttacc
    17761 tagaaaataa tcataaatac ttgccttcta ccgaatacct gcgttatact agtatcaatt
    17821 acttgttttt agcattcaaa ctatcaagga ggggatatga aatataggaa atttcaatta
    17881 ttgatgtcca agtatggctt tagtctttcg attatgctac ttgaactttg tcttgttttt
    17941 ggtctctttc tttatttagg acgcatggct cccattttat ggattactgt cctcattcta
    18001 ctgagtatca tcacaatcat ttcgatagtc aaccgtaata cgactcctga gaataaggta
    18061 acctggttgt tagtagcctt tgtgccagta tttggtccct tgctctatct gatgtttggt
    18121 gaaaggcgat tgtccaaaaa agaaatcaaa caactgaaga agctaggctc tatgcatttc
    18181 caagaagcaa atagccagct actaaaagag aaattaaaag aaagtgacaa ggcagcttat
    18241 ggagtcatca agtccttatt gagtatggat accaatgctg acatctatga tcaaactgcc
    18301 tctacatttt ttcctaacgg agaagctatg tggaaaaaga tggtagaaga tcttaaaaag
    18361 gctgagaaat ttattttctt ggaatattac attatagaag aaggtttgat gtggaatcgc
    18421 atactagata tactagagca aaaggtagct cagggtgtag aggttaagat gctctatgat
    18481 gatatcggct gtatggctac tttaacagga gattatgcac atcgacttcg tcagctgggc
    18541 atcgaggccc ataaattcaa taaagttatt cctcgtttga cagtggctta taataacaga
    18601 gatcatagaa aaatattgat tgttgatggt cagatagcct atactggtgg ggtcaatctg
    18661 gcagatgagt acattaacca cgtcgagaga tttggttatt ggaaggatag tggaattcgc
    18721 ttagacggac tagcagtaaa agctctgaca cgcttatttt tgaccacttg gtacattaat
    18781 cgaggagaaa ttagtgattt tgatcaatat catttagaaa atcattctat cccgagtgac
    18841 ggtttaacca ttccatacgg aagtggaccc aagccaattt ttcgagcgca ggtagggaaa
    18901 aaagtttatc agagtttaat caatcaagca acagaatcgg tctatattac gacaccttat
    18961 ttgattatag attatgattt aacagagaca atcaaaaatg cagctatgag aggggtcgat
    19021 gttcgaatta tcacccctta cataccagat aagaagttca ttcagttagt cacgagagga
    19081 gcttatcccg accttctttc tgctggtgtt cggatttatg agtatagtcc aggttttatt
    19141 catagtaagc agatgttggt agacgaagat tttgcggtgg tggggacaat caatctcgac
    19201 taccggagct tggtacacca ttatgaaaat gcagtcttac tctataaaac tccttctata
    19261 agggaaatcg cccgagattt tcgaaatata tttgcagatt ctcaggaagt ctatcctcat
    19321 tctatcaaaa cgagctggta tcaaaagctt gtaaaagaaa tcgcccaact attcgcccct
    19381 atcttataag aaatctagaa cttaggatac aaacccgacc tatttttctt gtcttttacg
    19441 aatagaaaga aagaggaggg gaattcatgc taactaagga agaagtgaat gatatgattg
    19501 agttcaaaca gacacattta caccgctttc gggaaattga aacctttgtg aaactatgca
    19561 aaaaatcact caaacagcca tcgcaagctg acaatcctag gactttttga tatactaaga
    19621 cagataaatt gaacaggaga aacgatatga gtgtgtatgg tagagtagaa gaagttcata
    19681 aggagaatcg tgaacctcta gaataccaaa ttgaacaaga atcgcatcat cgtgaatcaa
    19741 gtcgtcttcc tttggtgaaa attttactat ggagtacgct tgtaacgggg ataactctag
    19801 gagtaccgct attactcgac ttaatgagtg cacaagaagt gcaggatttt tatgcaggtt
    19861 gggcgcttca tcagacaggg aagatttaca gcgactatta tggaagtcaa ggtttgcttt
    19921 attatttgct gacttacgtg agtcagggcg gatttttctt tgccatcttt gagtggttag
    19981 ccttggtagc aggcggattt ttccttttta gatcagcgga caccttgaca aatcaaggag
    20041 accaagctgg acaactggtg actatttttt acatgctagt tacaggtctt gcttttggtg
    20101 gaggctatgc gactctttta gcgcttcctt tcttattcgc agcctttagt ttagttgcgg
    20161 cttacctaag caatccaagc catgataagg gatttgtacg gattgggcta gctttggcag
    20221 gcggattttt ctttgctccc ttatcatcgc tcctgtttat tgctgtagtg agtttaggct
    20281 tgttggtctt taaccttggg catagacgct ttgcgcatgg gttttatcag tttcttgcag
    20341 tggctttagg tttttcactt gtcttttatc caactgccta ctatagtgct gcaacaggaa
    20401 gttttgggga tgcgattagt ggtattcgtt atcctattga cagtattcgc tttgatttta
    20461 cttctaaaat tttagagaat atgttttttt atggcttgtt gtcccttggt ttgggatttg
    20521 tggtttgtat ctttttaggt ctctttcaat ccaaaccctt taaactatac gtcatttcgg
    20581 ttcctgcaag tcttgtggta attttaggtt tgattttact tttcttttca caagagcctc
    20641 tgcacgcttc ttatttgatg gtcgtcttcc ctgttttcct acttttattg gtaaccaata
    20701 ttaagagtca acagaggggg cgtagtgcta gaagaagccg aagagaaacg ccagttagcc
    20761 tatggagtcg tttcttcaaa ggaaatctat atctgctagt ttttgggttt gtctatcttt
    20821 tgtctgttcc ttttttgatg aagtttgtcc tttatccagt accttatcaa gaacgtaatc
    20881 gtcttgctga tttggtaaaa gaggagacaa atacggaaga tgctatctat gcatgggatg
    20941 atactgcgac tctttatcgt aagagtgagc gcttgtcgcc atcggcgatt ttgtccccgt
    21001 tgcactatac agcaactgag gaaaatcgta ataagttact taatgacttg aaagaaaaac
    21061 aacctaaggt gattgtggta aatgacaagg tggaagtctg gtctgaagtg gaaacactct
    21121 taaaagaaaa ttaccaacaa gtaaagactg attactcaga gtttaaagtc tataaaatta
    21181 aataaccaaa tcaatatctt gtgtattttt aaaaatttta agatttttaa cacaagatat
    21241 tgatttttct ttttagagtg gtataatact ttttagaaag aacattttag aaaagagcat
    21301 gcatatgatt gcactagaag aaaaaattac aattttgcca actctcttcg tcgagaaacg
    21361 agatgggaga cgtgttgtat ttgatgtgga caagattgac aaggctctcc acaaggcagc
    21421 tgacaaggtt atggatgtga cacccctggt tgaaaaacgc ctcaatgacc tgactgagag
    21481 aattattaca gaaattcata gtcgctttcc acagggaatt aagatttacg aaattcaaaa
    21541 tatcgtagaa catgaactcc ttgaagccaa agaatatgcg ctggctgagg agtatattac
    21601 ttatcggaca cagagggatt ttgagcgctc aaaagcgacg gatatcaact ttagtattca
    21661 taaacttctc aacaaagacc agacagttgt caatgaaaat gctaataaag acagtgatgt
    21721 ctttaacact cagcgtgatt tgacagcagg gattgttggg aaatcaatcg gactgcaaat
    21781 gcttcctaag cacgtagcca atgcccacca aaagggggat atccactatc acgatttgga
    21841 ctacagtccc tataccccta tgaccaactg ctgtttgatt gattttaagg gtatgttgga
    21901 aaatggtttt aagattggaa atgcagaggt agagagtccc aagtctatcc agactgcgac
    21961 agcacagatt tctcaaatca ttgccaacgt tgcttctagc cagtacggtg gctgttcagc
    22021 tgaccgtatc gatgaaattt tggcgcctta tgcagagaag aactatcaaa aacatctcaa
    22081 agatgcagaa gagtgggtat tgcctgaaaa acgggaagat tacgcttgga agaaaacgca
    22141 aaaggacatc tacgatgcca tgcaatctct tgagtatgaa atcaatactc tcttcacttc
    22201 aaatggacaa acacctttta cttcgctagg ttttggtctg ggaaccagtc gttttgaacg
    22261 agaaattcaa aaagctattt taaacattcg catcaagggt cttggttcag aacaccgtac
    22321 ggctatcttt cctaaactta ttttcactct gaaaagaggc cttaatttag aagaaggaac
    22381 tcccaactac gacatcaagc agttggctct tgagtgtgca accaaacgga tgtatccaga
    22441 tgttttatct tacgataaga ttgttgactt gacaggatcc ttcaaggtgc ctatgggttg
    22501 ccgttctttc ctccaaggat ggaaggatga aaatggtgta gaagtcaatt caggtcgcat
    22561 gaatctgggt gttgtgacgg ttaatctgcc tcgtattgct cttgagtctg aaggtgatat
    22621 gaataagttc tgggaaatct tcaacgagcg aatgaatatc gcagaagatg ctcttgttta
    22681 ccgtgtcgaa cgcactaaag aggcgacacc agcgaatgct cctatccttt atcagtacgg
    22741 tgcttttggc catcgtctag gtaaagaaga aagtgttgac cagctcttta agaatcgtcg
    22801 tgcgaccgtt tcgctgggct atatcggctt gtatgaagta gcgacagttt tctttggtaa
    22861 cagctgggaa agtaatccag atgctaagga attcacgcta gacatcattc acgatatgaa
    22921 acgccgtgta gaagagtggt cagaccaata tggctaccat ttctctatct actcaacacc
    22981 atccgaaagt ctgacagacc gtttctgccg actagatata gacaagtttg gctctattcc
    23041 tgatatcaca gacaaggaat actacaccaa ctctttccac tacgatgttc gtaaaaatcc
    23101 aacaccgttt gaaaaattgg actttgagaa agtctatcca gaagcaggtg cgtcaggtgg
    23161 tttcatccat tattgtgagt atccagtcct tcagcaaaat ccaaaggcct tggaagctgt
    23221 ctgggattat gcttatgacc gtgtaggcta tctaggcacc aatactccga ttgaccgttg
    23281 ctacaagtgt gactttgaag gggattttga accaactgag agagggtttg cttgtccaaa
    23341 ctgtggcaat agcgacccta aaacagtaga tgtggtgaag cgaacttgtg gctacctagg
    23401 taatcctcaa gcaagaccga tggtcaacgg gcgtcacaag gaaatcgctg cgcgtgtcaa
    23461 acatatgaat ggttcaacga ttaaaatagc tgggcatcaa gtaacaaatt agaaagaaat
    23521 gaaatgggaa aatatcaact agacgataag gggcgcgcac aagtgacccg ttatcacgag
    23581 aaacactcta aaggtggagc tggtaagaaa gaacgcttgc ttagcttcag agaacaattt
    23641 ttaaacaaga acaagaaaaa ataaaagtga gagccagctc tcgcttttct catagtggga
    23701 ggtaaggatg gaattacgca gaccaagatt agcggataag aaagctgttt tagatatgat
    23761 gacagagttt gaaaagagcc aatcagccca tgatggagga ttttgggata cagagaattt
    23821 tgtgtatgaa gagtggttgg aaagcaatca ggaacaggaa atggggatta atctgcctga
    23881 aggatgggtt cctgcaattc agttagtggc tttttctgag aaaggtcaag cagttggatt
    23941 tcttaatctc cggttgcgcc tcagtaactt tctactagaa gaaggtgggc acattggcta
    24001 ctccatccgt ccatctgaaa gaggcaaggg ttatgcaaaa gagactctcc gtcagggctt
    24061 gcaagttgct aaggaaaaga acatcaagaa agctctggtg acctgtagcg tgaataatcc
    24121 tgctagcaga gcagtcattc tagcaaatgg tggaatattt gaggatgctc acaatggagt
    24181 cgagcgttat tggatagagg tagcgaatga ataatccaaa accacaagaa tggaaaagcg
    24241 aggaacttag tcaaggtcgt atcattgact acaaggcctt taactttgtt gatggcgaag
    24301 gcgtgcgtaa ctctctctat gtatcaggct gtatgtttca ctgcgaggga tgttataatg
    24361 ttgcgacttg gtcttttaat gctggcattc tctatacagc agaattagaa gagcagatta
    24421 tggcagacct tgcccaaccc tatgttcaag gcttgacttt gctgggaggg gagccttttc
    24481 ttaatactgg cattctcttg cctctagtta aacgcatccg aaaggaattg ccagacaagg
    24541 acatttggtc ctggaccggc tacacttggg aagaaatgat gttggaaact ccagataaac
    24601 tggaactctt atcattgatt gacattcttg tcgatggacg atacgatcga actaagagaa
    24661 atctcatgct ccagtttcga ggttcgtcca accaacgaat tatcgatgtg caaaaatcgc
    24721 tcaaaagtgg gcaagtagtg atttgggaca agctcaatga cggaaaagaa agctatgaac
    24781 aggtgaagag agaatgaaga aaaaagactt agtagaccaa ctagtctcag agatcgagac
    24841 ggggaaagtc aggacactgg gaatatacgg tcatggagct tcaggtaaat caacctttgc
    24901 acaggaattg taccaagctt tagattctac tacagtaaat ttgctagaga cagatcctta
    24961 tatcacctca ggacgccatc tggtagtacc caaggacgcg ccgaatcaaa aggtgacagc
    25021 cagtctgcca gtggcgcatg aactggagag tttgcagaga gatatccttg ccttgcaggc
    25081 gggtatggat gtcttgacaa ttgaagaacc ttggaaggct agtgaggtct tgtctggagc
    25141 caaaccaatt ttgattgtcg aagggatgtc tgttggcttt ctacccaagg aactctttga
    25201 aaaaaccatc tgtttctaca cggatgagga gaccgaatta aagcgacgcc ttgctagaga
    25261 tacgactgtg agaaatcgcg atgcatcctt tatattggct agccatcaga tgagacggga
    25321 gcagtatctg cgatactata aagaaactga gtctaaggcg gatatcttag tggaccaatc
    25381 agaagataaa tttgatgtca agaggactta acttatgtat agaagaaata tagaagaaaa
    25441 tccctaattt tagaaagaaa aagtaggaaa acagtttcat cccaaaaaaa cgaaaaaacc
    25501 taacaaatcc cttgcaatcg caggggattt gtgttattct attatggtgc tgtaaattac
    25561 agccttagtt ttgatgcaag aggttgcgac acgctcggtt gcattgccac gcaacaccgc
    25621 gtcggttttc ttgtggagct agcctattat cttaaataga cgaaaaggag aaaaagatgg
    25681 caaacaaaaa aatccgtatc cgtttgaaag cttacgaaca ccgtacgctt gacacagcgg
    25741 ctgcaaaaat cgtagaatca gctactcgta caggtgcaca agttgcgggt ccaatcccac
    25801 ttccaactga acgtagcctc tacacaatca ttcgtgcgac tcacaaatac aaagactctc
    25861 gcgaacaatt tgaaatgcgt acacacaaac gtttgatcga tatcgttaac ccaactcaaa
    25921 aaacagttga tgccttgatg aaattggatc ttccaagtgg tgtaaacgta gaaatcaaac
    25981 tttaatctaa aatataaaag agcagaggct ggtgtttcaa tctaattgaa cacggactaa
    26041 actcggtgtg aaaaagataa acttcctagt gtctgctaga cactgcgtca gtttcctatt
    26101 ttcactttga gtttgacgcc ctttgtatct tagacttgag cataaaaaac gctcgttaaa
    26161 aactttttga ataaaaaata tagaaaagga actattttct catgacaaaa ggaatcttag
    26221 ggaaaaaagt gggaatgact caaatcttca ctgaagctgg cgaattgatc cctgtaacag
    26281 ttattgaagc aactccaaac gttgttcttc aagttaaaac tgttgaaaca gacggataca
    26341 acgctatcca agttggtttc gatgacaaac gcgaagtatt gagcaacaaa cctgctaaag
    26401 gacatgtagc gaaagctaac acggctccta agcgcttcat tcgtgaattc aaaaacgttg
    26461 aaggcttgga agttggtgct gaaattacag ttgaaacatt cgcagctgga gacgttgttg
    26521 acgtaactgg tacttctaaa ggtaaaggtt tccaaggtgt tatcaaacgc cacggacaat
    26581 cacgtggacc aatggctcac ggttctcgtt accaccgtcg tccaggttct atgggacctg
    26641 ttgcacctaa ccgcgtattc aaaggtaaaa accttgcagg gcgtatgggt ggcgaccgcg
    26701 taacaattca aaaccttgaa gttgtacaag ttgttccaga aaagaacgtt atccttatca
    26761 aaggtaacgt accaggtgct aagaaatctc ttatcactat caaatcagca gttaaagctg
    26821 gtaaataata aagaaagggg aaatcagtca caatggcaaa cgtaacatta tttgaccaaa
    26881 ctggtaaaga agctggccaa gttgttctta gcgatgcagt atttggtatc gaaccaaatg
    26941 aatcagttgt gtttgatgta atcatcagcc aacgcgcaag ccttcgtcaa ggaacacacg
    27001 ctgttaaaaa ccgctctgca gtatcaggtg gtggacgcaa accatggcgt caaaaaggaa
    27061 ctggacgtgc tcgtcaaggt tctatccgct caccacaatg gcgtggtggt ggtgttgtct
    27121 tcggaccaac tccacgttca tacggctaca aacttccaca aaaagttcgt cgcctagctc
    27181 ttaaatcagt ttactctgaa aaagttgctg aaaacaaatt cgtagctgta gacgctcttt
    27241 catttacagc tccaaaaact gctgaatttg caaaagttct tgcagcattg agcatcgatt
    27301 ctaaagttct tgttatcctt gaagaaggaa atgaattcgc agctctttca gctcgtaacc
    27361 ttccaaacgt gaaagttgca actgctacaa ctgcaagtgt tcttgacatc gcaaatagcg
    27421 acaaacttct tgtcacacaa gcagctatct ctaaaatcga ggaggttctt gcataatgaa
    27481 tttgtatgat gttatcaaaa aacctgtcat cactgaaagc tcaatggctc aacttgaagc
    27541 agggaaatat gtatttgaag ttgacactcg tgcacacaaa cttttgatca agcaagctgt
    27601 tgaagctgct ttcgaaggtg ttaaagttgc caatgttaac acaatcaacg taaaaccaaa
    27661 agctaaacgt gttggacgtt acactggttt tactaacaaa actaaaaaag ctatcatcac
    27721 acttacagct gattctaaag caatcgagtt gtttgctgct gaagctgaat aatctaagga
    27781 ggaaatatcg tgggaattcg tgtttataaa ccaacaacaa acggtcgccg taatatgact
    27841 tctttggatt tcgctgaaat cacaacaagc actcctgaaa aatcattgct tgttgcattg
    27901 aagagcaagg ctggtcgtaa caacaacggt cgtatcacag ttcgtcacca aggtggtgga
    27961 cacaaacgtt tctaccgttt ggttgacttc aaacgtaata aagacaacgt tgaagcagtt
    28021 gttaaaacaa tcgagtacga tccaaaccgt tctgcaaaca tcgctcttgt acactacact
    28081 gacggtgtga aagcatacat catcgctcca aaaggtcttg aagtaggtca acgtatcgtt
    28141 tcaggtccag aagcagatat caaagtcgga aacgctcttc cacttgctaa catcccagtt
    28201 ggtactttga ttcacaacat cgagttgaaa ccaggtcgtg gtggtgaatt ggtacgtgct
    28261 gctggtgcat ctgctcaagt attgggttct gaaggtaaat atgttcttgt tcgtcttcaa
    28321 tcaggtgaag ttcgtatgat tcttggaact tgccgtgcta cagttggtgt tgtcggaaac
    28381 gaacaacatg gacttgtaaa ccttggtaaa gcaggacgta gccgttggaa aggtatccgc
    28441 ccaacagttc gtggttctgt aatgaaccct aacgatcacc cacacggtgg tggtgaaggt
    28501 aaagcaccag ttggtcgtaa agcaccatct actccatggg gcaaacctgc tcttggtctt
    28561 aaaactcgta acaagaaagc gaaatctgac aaacttatcg ttcgtcgtcg caacgagaaa
    28621 taatattaaa ctagtcgctt aagcaactag taaatccgcc agctcggtag cgctccatag
    28681 gagtgcaagc cgctgtggta caacatttaa aggagaaaat ataaaaatgg gacgcagtct
    28741 taaaaaagga cctttcgtcg atgagcattt gatgaaaaaa gttgaagctc aagctaacga
    28801 cgaaaagaaa aaagttatta aaacttggtc acgtcgttca acgatcttcc caagtttcat
    28861 tggttacact attgcagttt atgacggacg taaacacgta cctgtttaca tccaagaaga
    28921 catggtaggc cacaaacttg gtgaatttgc accaactcgt acttacaaag gtcacgctgc
    28981 agacgacaag aaaacacgta gaaaataagg agaacataaa tggcagaaat tacttcagct
    29041 aaagcaatgg ctcgtacagt acgtgtttca cctcgtaaat cacgtcttgt tcttgataac
    29101 atccgtggta aaagcgtagc cgatgcaatc gcaatcttga cattcactcc aaacaaagct
    29161 gctgaaatca tcttgaaagt tttgaactca gctgtagcta acgctgaaaa caactttggt
    29221 ttggataaag ctaacttggt agtatctgaa gcattcgcaa acgaaggacc aactatgaaa
    29281 cgtttccgtc cacgtgcgaa aggttcagct tcaccaatca acaaacgtac agctcacatc
    29341 actgtagctg ttgcagaaaa ataaggaggt aaaatcgtgg gtcaaaaagt acatccaatt
    29401 ggtatgcgtg tcggcatcat ccgtgattgg gatgccaaat ggtatgctga aaaagaatac
    29461 gcggattacc ttcatgaaga tcttgcaatc cgtaaattcg ttcaaaaaga acttgctgac
    29521 gcagcagttt caactattga aatcgaacgc gcagtaaaca aagttaacgt ttcacttcac
    29581 actgctaaac caggtatggt tatcggtaaa ggtggtgcta atgttgatgc actccgtgca
    29641 aaacttaaca aattgactgg aaaacaagta cacatcaaca tcatcgaaat caaacaacct
    29701 gatttggatg ctcaccttgt aggtgaagga attgctcgtc aattggagca acgtgttgct
    29761 ttccgtcgtg cacaaaaaca agcaatccaa cgtgcaatgc gtgctggagc taaaggaatc
    29821 aaaactcaag tatcaggtcg tttgaacggt gcagatatcg cccgtgctga aggatactct
    29881 gaaggaactg ttccgcttca cacacttcgt gcagatatcg attacgcttg ggaagaagca
    29941 gatactacat acggtaaact tggtgttaaa gtatggatct accgtggtga agttcttcca
    30001 gctcgtaaaa acactaaagg aggtaaataa ccaatgttag tacctaaacg tgttaaacac
    30061 cgtcgtgagt tccgtggaaa aatgcgcggt gaagcaaaag gtggaaaaga agtagcattc
    30121 ggtgaatacg gtcttcaagc tacaactagc cactggatca ctaaccgcca aatcgaagct
    30181 gctcgtatcg ccatgactcg ttacatgaaa cgtggtggta aagtttggat taaaatcttc
    30241 ccacacaaat catacactgc taaagctatc ggtgtgcgta tgggatctgg taaaggggca
    30301 cctgaaggtt gggtagcacc agttaaacgt ggtaaagtga tgttcgaaat cgctggtgta
    30361 tctgaagaga ttgcacgtga agcgcttcga cttgctagcc acaaattgcc agttaaatgt
    30421 aaattcgtaa aacgtgaagc agaataagga gaaggcatga aacttaatga agtaaaagaa
    30481 tttgttaaag aacttcgtgg tctttctcaa gaagaactcg cgaagcgcga aaacgaattg
    30541 aaaaaagaat tgtttgaact tcgtttccaa gctgctactg gtcaattgga acaaacagct
    30601 cgcttgaaag aagttaaaaa acaaatcgct cgtatcaaaa cagttcaatc tgaagcgaaa
    30661 taatagacta gggaaggaga aatttcaatg gaacgcaata atcgtaaagt tcttgttgga
    30721 cgtgttgtat ctgacaaaat ggacaagaca atcacagttg tagttgaaac aaaacgtaac
    30781 cacccagtct atggtaaacg tattaactac tctaaaaaat acaaagctca tgatgaaaac
    30841 aatgttgcca aagaaggcga tatcgtacgt atcatggaaa ctcgcccgct ttcagctaca
    30901 aaacgtttcc gtcttgtaga agttgttgaa gaagcggtca tcatctaatc aaacctgaaa
    30961 ggagaaaact gaaatgattc aaacagaaac tcgtttgaaa gtcgcagaca acagcggtgc
    31021 tcgcgaaatc ttgactatca aagttcttgg tggttcagga cgtaaatttg caaacatcgg
    31081 tgatgttatc gtggcatctg taaaacaagc tactcctggt ggtgcggtta aaaaaggtga
    31141 cgttgttaaa gcagttatcg ttcgtactaa atcaggtgct cgtcgtgctg atggttcata
    31201 catcaaattt gacgaaaacg cagcagttat catccgtgaa gacaaaactc ctcgcggaac
    31261 acgtatcttt ggcccagttg cacgtgaatt gcgtgaaggt ggcttcatga agatcgtgtc
    31321 acttgctcca gaagtacttt aatttttagg aacaaactag tcccctagct tcaagctagg
    31381 gtgcccttat gggcgtaaga aaaatcaagg agaaacctaa tgtttgtaaa aaaaggcgac
    31441 aaagttcgcg taatcgctgg taaagataag ggaacagaag ctgttgtcct tactgccctt
    31501 ccaaaagtaa acaaagttat cgttgaaggt gttaacattg ttaagaaaca ccaacgtcca
    31561 actaacgagc ttcctcaagg tggtatcatc gagaaagaag cagctatcca cgtatcaaac
    31621 gttcaagttt tggacaaaaa tggtgtagct ggtcgtgttg gatacaaatt tgtagacggt
    31681 aaaaaagttc gctacaacaa aaaatcaggc gaagtgcttg attaatcacg aaggaaagga
    31741 gaagtataat ggcaaatcgt ttaaaagaaa aatatcttaa tgaagtagtt cctgctttga
    31801 cagaacaatt caactactca tcagtgatgg ctgtgcctaa agtagataag attgttttga
    31861 acatgggtgt tggtgaagct gtatcaaacg ctaaaagcct tgaaaaagct gctgaagaat
    31921 tggcacttat ctcaggtcaa aaaccactta tcactaaagc taaaaaatca atcgccggct
    31981 tccgtcttcg tgaaggtgtt gcgatcggtg caaaagttac ccttcgtggt gaacgtatgt
    32041 acgaattctt ggataaattg gtatcagttt cacttccgcg tgtacgtgac ttccacggtg
    32101 ttccaacaaa atcatttgat ggacgcggga actacacact tggtgtgaaa gaacaattaa
    32161 tcttcccaga aattaacttc gatgacgttg acaaaactcg tggtcttgac atcgttatcg
    32221 taacaactgc taacactgac gaagagtcac gtgcattgct tacaggcctt ggaatgcctt
    32281 ttgcaaaata atataggagg taaatctaat ggctaaaaaa tcaatggtag ctagagaggc
    32341 taaacgccaa aaaattgttg accgttatgc tgaaaaacgt gctgcattaa aggcggcagg
    32401 ggactacgaa ggtttatcta aattacctcg caacgcctca ccgactcgtt tacataatcg
    32461 ttgtagagtt acggggcgcc cacattcagt ttaccgcaaa tttggtctga gtcgtatcgc
    32521 ttttcgcgaa cttgcgcata aaggtcaaat tcctggtgta acaaaagcat cttggtaatt
    32581 taagatatca agagcgtcaa aactccaagt aaaaatagga aacttgacga agaaactaaa
    32641 gtttctagga aagtttatct ttttcacaca gagtttagcc cgggttcaat tgggcttgcc
    32701 aatttgaaca cgagctacag ctttggcaaa aaagaccaat ttgctttgga gcattgcttc
    32761 tgcattaaat tgtctatttt tgctcgtgct gttacgctct ttgtatcatg tattaactag
    32821 caagtgcaac ttgcaaacta ctagtaagag gagaaaaaca aaatggttat gactgaccca
    32881 atcgcagact tcctaactcg tattcgtaat gctaaccaag ctaaacacga agtacttgaa
    32941 gtacctgcat caaacatcaa aaaagggatt gctgaaatcc ttaaacgcga aggttttgta
    33001 aaaaacgttg aaatcattga agatgacaaa caaggcgtca tccgtgtatt tcttaaatac
    33061 ggaccaaatg gtgagaaagt tatcactaac ttgaaacgtg tttctaaacc aggacttcgt
    33121 gtctacaaaa aacgtgaaga ccttccaaaa gttcttaacg gacttggaat tgccatcctt
    33181 tcaacttctg aaggtttgct tactgataaa gaagcacgcc aaaagaatgt tggtggtgag
    33241 gttatcgctt acgtttggta aaatcaagat acaaagctcg taaagaacaa agcaaaatta
    33301 ggaagttgga gaagtttgtt tacaaacaag ccaacttatc tattttgcac agttcttaga
    33361 gcgtgttcag ttcagctctt gaactaaata agtatctgaa ccccgtgaaa actggccgtt
    33421 ttggcctgac aatttaacag gagaaaataa acatgtcacg tattggtaat aaagttatcg
    33481 tgttgcctgc tggtgttgaa ctcgctaaca atgacaacgt tgtaactgta aaaggaccta
    33541 aaggagaact tactcgtgag ttctcaaaag atattgaaat ccgtgtggaa ggtactgaag
    33601 taactcttca ccgtccaaac gattcaaaag aaatgaaaac tatccacgga actactcgtg
    33661 cccttttgaa caacatggtt gttggtgtat cagaaggatt caagaaagaa cttgaaatgc
    33721 gtggggttgg ttaccgtgca cagcttcaag gatctaaact tgttttggct gttggtaaat
    33781 ctcatccaga cgaagttgaa gctccagaag gaattacttt tgaacttcca aacccaacaa
    33841 caatcgttgt tagcggaatt tcaaaagaag tagttggtca aacagctgct tacgtacgta
    33901 gccttcgttc accagaacca tataaaggta aaggtatccg ttacgttggt gaattcgttc
    33961 gccgtaaaga aggtaaaaca ggtaaataat gttgagtggt tgatcatcaa ccaccaacct
    34021 attttccaac tttgtgcata gcacacgatt taaaactaaa gaggtgaaaa ctgtgatttc
    34081 aaaaccagat aaaaacaaac tccgccaaaa acgccaccgt cgcgttcgcg gaaaactctc
    34141 tggaactgct gatcgcccac gtttgaacgt attccgttct aatacaggca tctacgctca
    34201 agtgattgat gacgtagcgg gtgtaacgct cgcaagtgct tcaactcttg ataaagaagt
    34261 ttcaaaagga actaaaactg aacaagccgt tgctgtcggt aaactcgttg cagaacgtgc
    34321 aaacgctaaa ggtatttcag aagtggtgtt cgaccgcggt ggatatctat atcacggacg
    34381 tgtgaaagct ttggctgatg cagctcgtga aaacggattg aaattctaat aggaggacac
    34441 tagaaaatgg catttaaaga caatgcagtt gaattagaag aacgcgtagt tgctgtcaac
    34501 cgtgttacaa aagttgttaa aggtggacgt cgtcttcgtt tcgcagctct tgttgttgtt
    34561 ggtgaccaca atggtcgcgt aggatttggt actggtaaag ctcaagaagt tccagaagca
    34621 atccgtaaag cagtagatga tgctaagaaa aacttgatcg aagttcctat ggttggaaca
    34681 acaatcccac acgaagttct ttcagaattc ggtggagcta aagtattgtt gaaacctgct
    34741 gtagaaggtt ctggagttgc cgctggtggt gcagttcgtg ccgttgtgga attggcaggt
    34801 gtggcagata ttacatctaa atcacttggt tctaacactc caatcaacat tgttcgtgca
    34861 actgttgaag gtttgaaaca attgaaacgc gctgaagaaa ttgctgccct tcgtggtatt
    34921 tcagtttctg atttggcata agaaagggga taaaatggct caaattaaaa ttactttgac
    34981 taagtctcca atcggacgca ttccatcaca acgtaaaact gttgtagcac ttggacttgg
    35041 caaattgaac agctctgtta ttaaagaaga taacgctgct atccgtggta tgatcacagc
    35101 agtatctcac ttagtaacag ttgaagaagt aaactaatga agttttaggg gatgtgcact
    35161 gtaccatccc ctaaaactag atatagtcat ctatgatgac atcgtatagg cgagttgatg
    35221 ggggagacaa ccttttctcc cttatcggcg ctagcatttt acaaaagagg agaaaataaa
    35281 aatgaaactt catgaattga aacctgcaga aggttctcgt aaagtacgta accgcgttgg
    35341 tcgtggtact tcatcaggta acggtaaaac atctggtcgt ggtcaaaaag gtcaaaaagc
    35401 tcgtagcggt ggcggagttc gccttggttt tgaaggtgga caaactccat tgttccgtcg
    35461 tcttccaaaa cgtggattca ctaacatcaa cgctaaagaa tacgcaattg tgaaccttga
    35521 ccaattgaac gtctttgaag atggtgctga agtaactcca gttgttctta tcgaagcagg
    35581 aattgttaaa gctgaaaagt caggtattaa aattcttggt aacggtgagt tgactaagaa
    35641 attgactgtg aaagcagcta aattctctaa atcagctgaa gaagctatca ctgctaaagg
    35701 tggttcagta gaagtcatct aagagaggtg acctatgttt tttaaattat taagagaagc
    35761 tcttaaagtc aagcaggttc gatcaaaaat tttatttaca atttttatcg ttttggtctt
    35821 tcgtatcgga actagcatta cagttcctgg tgtgaatgcc aatagcttga atgctttaag
    35881 tggattatcc ttcttaaaca tgttgagctt ggtgtcgggg aatgccctaa aaaacttttc
    35941 gatttttgcc ctaggagtta gtccctatat caccgcttct attgttgtcc aactcttgca
    36001 aatggatatt ttacccaagt ttgtagagtg gggtaaacaa ggggaagtag gtcgaagaaa
    36061 attgaatcaa gctactcgtt atattgctct agttctcgct tttgtgcaat ctatcgggat
    36121 tacagctggt tttaatacct tggctggagc tcaattgatt aaaactgctt taactccaca
    36181 agtttttctg acgattggta tcatcttaac agctggtagt atgattgtca cttggttggg
    36241 tgagcaaatt acagataagg gatacggaaa cggtgtttcc atgattatct ttgccgggat
    36301 tgtttcctca attccagaga tgattcaggg catctatgtg gactactttg tgaacgtccc
    36361 aagtagccgt atcacttcat ctatcatttt cgtaatcatt ttgattatta ctgtattgtt
    36421 gattatttac tttacaactt atgttcaaca agcagaatac aaaattccaa tccaatatac
    36481 taaggttgca caaggtgctc catctagctc ttaccttccg ttaaaggtaa atcctgctgg
    36541 agttatccct gttatctttg ccagttcgat tactgcagcg cctgcggcta ttcttcagtt
    36601 tttgagtgcc acaggtcatg attgggcttg ggtaagggta gcacaagaga tgttggcaac
    36661 tacttctcca actggtattg ccatgtatgc tttgttgatt attctcttta cattcttcta
    36721 tacgtttgta cagattaatc ctgaaaaagc agcagagagc ctacaaaaga gtggtgccta
    36781 tatccatgga gttcgtcctg gtaaaggtac agaagaatat atgtctaaac ttcttcgtcg
    36841 tcttgcaact gttggttccc tcttccttgg tgtgatttcc attttaccga ttgcagctaa
    36901 agatgtattt ggtctttctg atgttgttgc ctttggtgga acaagtctct tgatcattat
    36961 ctctacaggt atcgaaggaa tcaagcaatt ggaaggttac ctattgaaac gtaagtatgt
    37021 tggtttcatg gacagaacag aataaaagta tttactgaat cagtaaatac tgagggagtg
    37081 gaggtttaaa ctctgacatt tgtaagagtt ggatctcccc tcttctattt tgtttttaaa
    37141 tcggggtgaa aaaacttttt gcttctattt aaaaacaaaa taaggagatc aaattatgaa
    37201 tcttttgatt atgggcttac ctggtgcagg taagggaact caagcagcaa aaatcgtaga
    37261 acaattccat gttgcacata tctcaacagg tgatatgttc cgcgctgcaa tggcaaatca
    37321 aactgaaatg ggtgttcttg ctaagtcata tattgacaag ggtgaattgg ttcctgacga
    37381 agttacaaat ggaatcgtaa aagaacgcct ttcacaagat gatattaaag aaacaggatt
    37441 cttattggat ggttacccac gtacaattga acaagctcat gccttggaca aaacattggc
    37501 tgaacttggc attgaactag aaggtattat caatattgaa gttaaccctg acagcctctt
    37561 ggaacgtttg agtggccgta tcatccaccg cgtaactgga gaaactttcc acaaggtctt
    37621 taacccacca gttgactata aagaagaaga ttactaccaa cgtgaagatg ataagcctga
    37681 gacagtaaaa cgtcgtttgg atgttaatat tgctcaagga gaaccaatca ttgctcacta
    37741 ccgtgccaaa ggtttggttc atgacatcga aggtaatcaa gatatcaatg atgtcttctc
    37801 agatattgaa aaagtattga caaatttgaa ataaagcgtt tttcacactt gcaaaaatcc
    37861 gctacaaatg ttatactgaa atagtctgac ttataattgt tgtctctgtg tctagaggca
    37921 tcgaatcgaa atttatggag gtgcttttgc gtggcaaaag acgatgtgat tgaagttgaa
    37981 ggcaaagtag ttgatacaat gccgaatgca atgtttacgg ttgaacttga aaatggacat
    38041 cagattttag caacagtttc tggtaaaatt cgtaaaaact atattcgtat tttagcggga
    38101 gatcgtgtta ctgtcgaaat gagtccatat gacttgacac gtggacgtat cacttaccgc
    38161 tttaaataat cgaaaaactt ggagggataa gaaatgaaag taagaccatc ggtcaaacca
    38221 atttgcgaat actgtaaagt tattcgtcgt aatggtcgtg ttatggtaat ttgcccagca
    38281 aatccaaaac acaaacaacg tcaaggataa gatagaaagg agaaaacatg gctcgtattg
    38341 ctggagttga tattccaaat gacaaacgcg tagtaatctc attgacttat gtttatggta
    38401 tcggacttgc aacatctaag aaaattttgg ctgctgctgg aatctcagaa gatgttcgtg
    38461 tacgtgatct tacatcagat caagaagatg ctatccgtcg tgaagtggat gcaatcaaag
    38521 ttgaaggtga ccttcgtcgt gaagtaaact tgaacatcaa acgtttgatg gaaatcggtt
    38581 cataccgtgg tatccgtcac cgtcgtggac ttcctgtccg tggacaaaat actaaaaaca
    38641 acgctcgcac tcgtaaaggt aaagctgttg cgattgctgg taagaaaaaa taatatagga
    38701 ggtaaaagtc ttggctaaac caacacgtaa acgtcgtgtg aaaaagaata tcgaatctgg
    38761 tattgctcat attcacgcta catttaataa cactattgtt atgattactg atgtgcatgg
    38821 taatgcaatt gcttggtcat cagctggtgc tcttggtttc aaaggttctc gtaaatctac
    38881 accattcgct gctcaaatgg cttctgaagc tgctgctaaa tctgcacaag aacacggtct
    38941 taaatcagtt gaagttactg taaaaggtcc aggttctggt cgtgagtcag ctattcgtgc
    39001 gcttgctgcc gctggtcttg aagtaacagc aattcgtgat gtgactccag tgccacacaa
    39061 tggtgctcgt cctccaaaac gtcgccgtgt ataatcatcg cattacactg cttttcgttt
    39121 aagagggagt aactaaatga tcgagtttga aaaaccaaat ataacaaaaa ttgatgaaaa
    39181 taaagattat ggcaagtttg taatcgaacc acttgaacgt ggctacggta caactcttgg
    39241 taactctctt cgtcgtgtac ttctagcttc tctaccagga gcagctgtga catctatcaa
    39301 cattgatggt gtgttacatg agtttgacac agttccaggt gttcgtgaag acgtgatgca
    39361 aatcattctg aacattaagg gaattgcagt gaaatcgtac gttgaagacg aaaaaatcat
    39421 cgaactggat gttgaaggtc ctgctgaagt aacagctggt gacattttga cagatagcga
    39481 tattgaaatt gtaaatccag atcattatct ctttacaatc ggtgaaggtt cttctctaaa
    39541 agcgactatg actgttaaca gtggtcgtgg atatgtacct gctgacgaaa ataaaaagga
    39601 taatgcacca gttggaacac ttgctgtaga ttctatttat acaccagtta caaaagtcaa
    39661 ctatcaagtg gaacctgctc gtgtaggtag caatgatggt ttcgacaaat taacccttga
    39721 aatcttgaca aatggaacaa ttattccaga agatgcttta gggctttcag cacgtatttt
    39781 gacagaacat cttgatttgt ttacaaatct tactgagatt gctaagtcaa ctgaagtgat
    39841 gaaagaagct gatactgaat ctgacgaccg tattttagat cgtacgattg aggaactgga
    39901 cttgtctgtg cgttcataca actgtttaaa acgtgccggt atcaatactg tgcatgattt
    39961 gacagaaaaa tctgaagcag agatgatgaa agtacgaaat cttggacgca agagtttgga
    40021 agaagtgaaa ctcaaactca ttgatttggg tcttggatta aaagataaat aaaggaggaa
    40081 tacatggctt accgtaaact aggacgcact agctcacaac gtaaagcaat gcttcgcgat
    40141 ttgacaactg accttttgat caacgaatca atcgtgacaa ctgaagctcg tgctaaagaa
    40201 atccgtaaaa ctgttgaaaa aatgattact ctaggtaaac gtggtgattt gcatgcacgt
    40261 cgtcaagcag ctgctttcgt acgtaatgaa atcgcatctg aaaactatga tgaagcaact
    40321 gataagtaca cttctactac agcacttcaa aaattgttct cagaaatcgc acctcgttat
    40381 gctgaacgta acggtggata cactcgtatc cttaaaactg aaccacgtcg tggtgatgca
    40441 gcgccaatgg cgatcatcga attagtataa aatcatcaat tttgttgagt gttatgatga
    40501 tggagtcttg tgctcttagt ctagctctgg tctaccgcta ggatttcggt cctagcggga
    40561 acactcatca taagttggga tagtagacgc ttgtttacga aattgttttt ttcttaagaa
    40621 caacttcgta agcaggcgtt tttgagtatt ttcgttagaa ttatgctata ctatttgaaa
    40681 agaatcctgt ttaatgttaa ggtttcttat tttaagaaga attggagttt acttatgaaa
    40741 gccattataa ctgttgttgg taaagataaa tctggaattg ttgcaggtgt ttctggtaaa
    40801 attgcagaat taggattgaa tattgacgat atctctcaaa ctgtattgga tgaatatttt
    40861 acgatgatgg ctgttgtatc tagtgatgaa aagcaagatt ttacctatct tcgtaatgaa
    40921 tttgaagctt ttgggcaaac tttgaatgta aaaatcaata ttcagagtgc agcgattttc
    40981 gaagctatgt ataatatcta ggaggtcatc atggatatta gacaagttac tgaaaccatc
    41041 gccatgattg aggagcaaaa cttcgatatt agaaccatta ccatggggat ttctcttttg
    41101 gactgtatcg atccaaatat caatcgtgct gcggagaaaa tctatcaaaa aattacgaca
    41161 aaggcggcta atttagtagc tgttggtgat gaaattgcgg ctgagttggg aattcctatc
    41221 gttaataagc gtgtatcggt gacacctatt tctctgattg gggcagcgac agatgcgacg
    41281 gactacgtgg ttctggcaaa agcgcttgat aaggctgcga aagagattgg tgtggacttt
    41341 attggtggtt tttctgcctt agtacaaaaa ggttatcaaa agggagatga gattctcatc
    41401 aattccattc ctcgcgcttt ggctgagacg gataaggtct gctcgtcagt caatatcggc
    41461 tcaaccaagt ctggtattaa tatgacggct gtggcagata tgggacgaat tatcaaggaa
    41521 acagcaaatc tttcagatat gggagtggcc aagttggttg tattcgctaa tgctgttgag
    41581 gacaatccat ttatggcggg tgcctttcat ggtgttgggg aagcagatgt tatcatcaat
    41641 gtcggagttt ctggtcctgg tgtggtgaaa cgtgctttgg aaaaagttcg tggacagagc
    41701 tttgatgtag tagccgaaac agttaagaaa actgccttta aaatcactcg tatcggtcaa
    41761 ttggttggtc aaatggccag tgagagactg ggtgtggagt ttggtattgt ggacttgagt
    41821 ttggcaccaa cccctgcggt tggagactct gtagcacgtg tccttgagga aatggggcta
    41881 gaaacagttg gcacgcatgg aacgacggct gccttggccc tcttgaacga ccaagttaaa
    41941 aagggtggag tgatggcctg caaccaagtc ggtggtttat ctggtgcctt tatccctgtt
    42001 tctgaggatg aaggaatgat tgctgcagtg caaaatggct ctcttaattt agaaaaacta
    42061 gaagctatga cggctatctg ttctgttgga ttggatatga ttgccatccc agaagatacg
    42121 cctgctgaaa ccattgcggc tatgattgcg gatgaagcag caatcggtgt tatcaacatg
    42181 aaaacaacag ctgttcgtat cattcccaaa ggaagagaag gcgatatgat tgagtttggt
    42241 ggtctattag gaactgcacc tgttatgaag gttaatgggg cttcgtctgt cgacttcatc
    42301 tctcgcggtg gacaaatccc agcaccaatt catagtttta aaaattaaga aaataggaga
    42361 aattttaagt tctatttaag at
//
LOCUS       DAVHIT010000006        39770 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVHIT010000006 DAVHIT010000000
VERSION     DAVHIT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.006"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            150..1379
                     /gene="arcA"
                     /locus_tag="VVN16_000253"
     CDS             150..1379
                     /gene="arcA"
                     /locus_tag="VVN16_000253"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU5571707.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1437..2453
                     /gene="argF"
                     /locus_tag="VVN16_000254"
     CDS             1437..2453
                     /gene="argF"
                     /locus_tag="VVN16_000254"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5571708.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2618..3565
                     /gene="arcC"
                     /locus_tag="VVN16_000255"
     CDS             2618..3565
                     /gene="arcC"
                     /locus_tag="VVN16_000255"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU5571709.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLERVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3776..5287
                     /locus_tag="VVN16_000256"
     CDS             3776..5287
                     /locus_tag="VVN16_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU5571710.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5309..6640
                     /locus_tag="VVN16_000257"
     CDS             5309..6640
                     /locus_tag="VVN16_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5571711.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLYGSGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            6735..6862
                     /locus_tag="VVN16_000258"
                     /pseudo
     CDS             6735..6862
                     /locus_tag="VVN16_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(7045..7336)
                     /locus_tag="VVN16_000259"
                     /pseudo
     CDS             complement(7045..7336)
                     /locus_tag="VVN16_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7324..7575)
                     /locus_tag="VVN16_000260"
     CDS             complement(7324..7575)
                     /locus_tag="VVN16_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU5571712.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(7929..8813)
                     /locus_tag="VVN16_000261"
     CDS             complement(7929..8813)
                     /locus_tag="VVN16_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5571713.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(8957..10108)
                     /locus_tag="VVN16_000262"
     CDS             complement(8957..10108)
                     /locus_tag="VVN16_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU5571714.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMAINGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGYDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10254..12020)
                     /locus_tag="VVN16_000263"
     CDS             complement(10254..12020)
                     /locus_tag="VVN16_000263"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU5571715.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(12033..12761)
                     /locus_tag="VVN16_000264"
     CDS             complement(12033..12761)
                     /locus_tag="VVN16_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU5571716.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            complement(12776..15793)
                     /locus_tag="VVN16_000265"
     CDS             complement(12776..15793)
                     /locus_tag="VVN16_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEU5571717.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINNNGNLEFELHF"
     gene            complement(15804..18020)
                     /locus_tag="VVN16_000266"
     CDS             complement(15804..18020)
                     /locus_tag="VVN16_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU5571718.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLLQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            complement(18042..19913)
                     /locus_tag="VVN16_000267"
     CDS             complement(18042..19913)
                     /locus_tag="VVN16_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEU5571719.1"
                     /translation="MKIENKNVRRNFFWGEGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTAVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDSQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANACAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            complement(19919..21238)
                     /locus_tag="VVN16_000268"
     CDS             complement(19919..21238)
                     /locus_tag="VVN16_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU5571720.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNENTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            complement(21235..22074)
                     /locus_tag="VVN16_000269"
     CDS             complement(21235..22074)
                     /locus_tag="VVN16_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5571721.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            complement(22077..23006)
                     /locus_tag="VVN16_000270"
     CDS             complement(22077..23006)
                     /locus_tag="VVN16_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5571722.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIFCINILFRERKRLKGGK"
     gene            complement(23093..24385)
                     /locus_tag="VVN16_000271"
     CDS             complement(23093..24385)
                     /locus_tag="VVN16_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5571723.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            complement(24414..25817)
                     /locus_tag="VVN16_000272"
     CDS             complement(24414..25817)
                     /locus_tag="VVN16_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU5571724.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLLSQGKINEDTELK
                     DIMTNIADTQYYYPQLS"
     gene            25993..26766
                     /locus_tag="VVN16_000273"
     CDS             25993..26766
                     /locus_tag="VVN16_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5571725.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(27937..29442)
                     /gene="adcA"
                     /locus_tag="VVN16_000274"
     CDS             complement(27937..29442)
                     /gene="adcA"
                     /locus_tag="VVN16_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU5571726.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIRATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(29452..30258)
                     /locus_tag="VVN16_000275"
     CDS             complement(29452..30258)
                     /locus_tag="VVN16_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU5571727.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(30251..30955)
                     /locus_tag="VVN16_000276"
     CDS             complement(30251..30955)
                     /locus_tag="VVN16_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU5571728.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(30955..31395)
                     /gene="adcR"
                     /locus_tag="VVN16_000277"
     CDS             complement(30955..31395)
                     /gene="adcR"
                     /locus_tag="VVN16_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU5571729.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(31550..32818)
                     /gene="dltD"
                     /locus_tag="VVN16_000278"
     CDS             complement(31550..32818)
                     /gene="dltD"
                     /locus_tag="VVN16_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU5571730.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(32811..33050)
                     /gene="dltC"
                     /locus_tag="VVN16_000279"
     CDS             complement(32811..33050)
                     /gene="dltC"
                     /locus_tag="VVN16_000279"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU5571731.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(33064..34308)
                     /gene="dltB"
                     /locus_tag="VVN16_000280"
     CDS             complement(33064..34308)
                     /gene="dltB"
                     /locus_tag="VVN16_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU5571732.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(34305..35855)
                     /gene="dltA"
                     /locus_tag="VVN16_000281"
     CDS             complement(34305..35855)
                     /gene="dltA"
                     /locus_tag="VVN16_000281"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU5571733.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(35876..36007)
                     /locus_tag="VVN16_000282"
     CDS             complement(35876..36007)
                     /locus_tag="VVN16_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU5571734.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(36321..37499)
                     /locus_tag="VVN16_000283"
     CDS             complement(36321..37499)
                     /locus_tag="VVN16_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5571735.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLLQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(37955..38263)
                     /locus_tag="VVN16_000284"
     CDS             complement(37955..38263)
                     /locus_tag="VVN16_000284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571736.1"
                     /translation="MDKLFLIGKYESIVASIDERLRTRLRVIIWKQWKKKSRRLWGLL
                     KLGVPKWIADKVSGWGDHYQLVAQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS
                     "
     gene            complement(38224..38775)
                     /locus_tag="VVN16_000285"
     CDS             complement(38224..38775)
                     /locus_tag="VVN16_000285"
                     /inference="COORDINATES: protein motif:HMM:NF019984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571737.1"
                     /translation="MSLVHNIIEDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNL
                     SPLLSNIMLNELDKELEKRGLRFVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLK
                     VNMTKTKITRPRELKYLGFGFWKSSDGWKSRPHQDSVRRFKLKLKKLTHRKWSIDLTR
                     RIEQLNLSIRGWINYFSLGNMKV"
     gene            complement(38817..39191)
                     /locus_tag="VVN16_000286"
     CDS             complement(38817..39191)
                     /locus_tag="VVN16_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase"
                     /protein_id="HEU5571738.1"
                     /translation="MLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQNWRLTKELIKQRK
                     YKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEPHFSDTSYGFRPNR
                     SCEKAIMKLLEYLNDGYEWIVD"
BASE COUNT        12209 a         8279 c         6771 g        12511 t
ORIGIN      
        1 ataataacat aaaattgtgc taaatatcat aatttatatt attttccgag aaattatagc
       61 cattgacatt acctaggaat tgcgttataa catacatgta agcggtaccc aaaatggtgc
      121 caagtcaaaa tttttaagga ggaaaataca tgtcttcaca tccaattcag gtcttctcag
      181 aaattgggaa actgaaaaaa gttatgttac accgtccagg caaggagtta gaaaacttgt
      241 tgccggacta tcttgaaagg cttctttttg atgatattcc tttcttggaa gatgctcaaa
      301 aagaacatga tgcatttgcc caagctcttc gcgatgaagg aattgaggtt ctctacctag
      361 aacaactcgc tgctgaatca ttgacctctc cagaaatccg cgatcaattt atcgaggaat
      421 acttagacga agccaacatc cgtgatcgtc aaaccaaggt tgctattcgt gaattacttc
      481 acggcatcaa ggacaaccaa gaattggttg aaaaaacaat ggctgggatt caaaaagttg
      541 aattgccaga aattcctgac gaagctaaag atctaactga cttagttgaa tccgattatc
      601 catttgcaat tgacccgatg ccaaacctct atttcactcg cgacccattt gcaacaattg
      661 gaaacgccgt atcgcttaac cacatgtttg cagacactcg taaccgtgaa acactctacg
      721 gtaagtatat cttcaaatac cacccaatct atggcggaaa agtggatttg gtctacaacc
      781 gtgaagaaga tacgcgtatc gaaggtggag acgagctagt tctttctaaa gacgtccttg
      841 cagtaggtat ctctcaacgt acagacgcag cttctatcga aaaacttttg gtcaacatct
      901 tcaagaaaaa tgttggcttc aagaaagttt tggcctttga atttgctaac aaccgtaaat
      961 tcatgcactt ggatactgtc ttcactatgg tagactatga caagttcact attcacccag
     1021 aaatcgaagg cgaccttcac gtttactcag ttacttacga aaacgaaaaa cttaaaatcg
     1081 ttgaagagaa aggtgactta gctgaacttc ttgctcaaaa ccttggtgta gaaaaagttc
     1141 atttgattcg ttgcggtggt ggcaatatcg tagcagctgc gcgtgaacaa tggaacgacg
     1201 gttctaacac tttgaccatc gcacctggtg tggtagttgt ttatgaccgc aataccgtga
     1261 ccaataagat tttggaagaa tacgggcttc gcttgattaa gattcgcgga agtgaattgg
     1321 ttcggggccg tggtggacct cgttgtatgt ctatgccatt tgaacgtgaa gaagtgtaat
     1381 cgctgttcgg tattcgtcaa tagaaaatgt aaaaaataga aagaggaaat aataaaatga
     1441 caaattcagt attccaagga cgcagcttct tagcagaaaa agactttacc cgtgcagagt
     1501 tagaatacct tattggtctt tcagctcact tgaaagattt gaaaaaacgc aatattcaac
     1561 accactacct tgctggcaag aatatcgctc tcctatttga aaaaacatct actcgtactc
     1621 gtgcagcctt tacaactgcg gctatcgacc ttggtgctca cccagaatac ctcggagcaa
     1681 atgatattca gttgggtaaa aaagaatcta ctgaagatac tgctaaagta ttgggacgta
     1741 tgtttgacgg gattgaattc cgcggattca gccaacgtat ggttgaagaa ttggcagaat
     1801 tctcaggcgt tccagtatgg aacggtctaa ctgacgaatg gcacccaact caaatgctcg
     1861 ctgactactt gactgttcaa gaaaacttcg gtcgcttgga aggcttgaca ttggtatact
     1921 gtggtgatgg acgtaacaac gttgccaaca gcttgctcgt aacaggtgct atccttggtg
     1981 tcaatgttca catcttctca ccaaaagaac tcttcccaga aaaagaaatc gttgaattgg
     2041 cagaaggatt tgctaaagaa agtggcgcac atgttctcat cactgaagat gctgatgaag
     2101 cagttaaaga tgcagacgtt ctttacacag acgtttgggt atcaatgggt gaagaagaca
     2161 aattcgcaga acgtgtagct cttcttaaac cttaccaagt caatatggac ttagttaaaa
     2221 aagcaggcaa tgaaaacttg atcttcctac actgcttgcc agcattccac gatactcaca
     2281 ctgtttatgg taaagacgtt gctgaaaaat ttggtgtaga agaaatggaa gtaacagacg
     2341 aagtcttccg cagcaagtac gctcgccact tcgatcaagc agaaaaccgt atgcacacta
     2401 tcaaagctgt tatggctgct acacttggta acctttatat tcctaaagta taattttaga
     2461 taataaaccg tctaccaaca gctatggggg ctgcgactaa tagctttagt ccggtcctct
     2521 tttatataat ggtaatctat tatttcttat aaaatatgtg aaaaatcatt aaattgaaat
     2581 ctaaacgcat tctattgagt gtgataaagg agaatttatg gcaaatcgta aaattgtagt
     2641 agctttggga ggaaatgcga ttctttcttc tgacccatca gcaaaagctc aacaagaagc
     2701 tttagttgaa acagctaagc atcttgtaaa attgattaaa aatggagatg atttgattat
     2761 cactcacggt aatggacctc aagttgggaa tctcttgctc caacatttgg catcagactc
     2821 tgaaaagaac cctgccttcc cactcgactc acttgtcgct atgacagaag gtagcatcgg
     2881 tttctggttg aaaaatgctt tgcaaaatgc tctcttagat gaaggcatcg aaaaaaatgt
     2941 tgcctctgtt gtaacgcaag ttgtcgtaga taaaaatgat ccagcttttg ttaacttgag
     3001 taaaccaatc ggtcctttct attcagaaga agaagcaaaa gcagaagccg aaaagagcgg
     3061 agcgactttc aaggaagatg ctggccgtgg ctggcgtaag gtcgttgcct caccaaaacc
     3121 tgttgacatc aaagaaattg aaaccatccg tactctttta aataatggtc aagtcgtcgt
     3181 agctgcaggt ggtggcggta ttcccgtcgt caaagaaaac aatggacatt tgactggtgt
     3241 cgaagcggtt attgataaag acttcgcttc ccaacgtttg gcagaattgg ttgatgcaga
     3301 cctcttcatc attttgacag gtgtagatta tgtatttgtt aactacaaca agccaaacca
     3361 ggaaaaattg gaacgtgtga atgttgccca gctggaagaa tatatcaaac aagatcagtt
     3421 tgcaccaggt agcatgcttc caaaagtaga agcagctatc gcttttgtca atggtcgtcc
     3481 agaaggaaaa gcagttatta cttcccttga aaatctaggc gccttgattg aatctgaaag
     3541 cggaacaatt attgaaaaag gataagttgt tttactaata agatgtattc tatttctagt
     3601 atctttatat caaattagaa attattcttg aaaacatgta caatatttca aaagatatta
     3661 gttttttaga ctttaatatg gtaaaacaaa tataaataga aagcgttttc ttgaatgttt
     3721 atttaagaaa gtagttggtt ttttacactt tgttagacat caggaggaaa aacaaatgag
     3781 tgaaaaagct aaaaaagggt ttaagatgcc ttcatcttac accgtattat tgataatcat
     3841 tgctattatg gcagtgctaa cttggtttat ccctgcgggg gcctttatag aaggtattta
     3901 cgagactcag cctcaaaatc cacaagggat ttgggatgtc ctcatggcac cgattcgggc
     3961 tatgctaggt actcatccag aggaaggttc gctcattaaa gaaacgagcg cagcgattga
     4021 tgtagccttc ttcatcctta tggttggggg tttccttggc attgtcaaca aaactggtgc
     4081 tcttgacgta gggattgcct ctatcgtgaa gaagtataag ggccgcgaaa aaatgttaat
     4141 tttggtactg atgcctttgt ttgccctcgg tggtacaact tatggtatgg gggaagaaac
     4201 aatggccttc tatccactcc ttgtgccagt tatgatggcc gttggttttg atagcctgac
     4261 tggtgttgca attattttgc tcggttctca aatcggctgt ttggcatcta ctctgaatcc
     4321 atttgcgaca ggtattgctt cagcgactgc gggagttggt acaggggacg gtatcgtact
     4381 tcgtctgatc ttctgggtta ccttgactgc tcttagtact tggtttgttt accgttatgc
     4441 ggataagatt caaaaagatc cgactaagtc actggtttat agtactcgca aagaagattt
     4501 gaaacacttt aacgtagaag aatcttcatc tgtagaatct acacttagca gcaaacaaaa
     4561 atcagttctc ttcttatttg tgttgacatt catcttgatg gtattgagct tcattccatg
     4621 gacagacctt ggcgttacca tttttgatga ctttaatgct tggttgactg gtcttccagt
     4681 tattggcaat attgtcggtt catctacttc tgcactaggt acttggtact tcccagaagg
     4741 cgcaatgctc tttgccttta tgggtatcct gattggtgtt atttatggtc ttaaagaaga
     4801 taagattatc tcttccttca tgaatggtgc tgctgacttg ctcagtgttg ccttgatcgt
     4861 agcgattgct cgtggtattc aagttatcat gaacgacggt atgattaccg atacaatcct
     4921 caactggggt aaagaaggct tgagcggtct atcttcacaa gtctttatcg ttgtaactta
     4981 tatcttctat ctacctatgt cattcttgat cccatcttca tctggtcttg ccagcgcaac
     5041 tatgggtatc atggctccac ttggagaatt tgtaaatgtc cgtcctagct tgattatcac
     5101 tgcttaccaa tctgcttcag gtgtcttgaa cttgattgca ccaacatctg gtattgtgat
     5161 gggagctctt gcacttggac gtatcaacat tggtacttgg tggaaattca tgggcaaact
     5221 cgtagtcgct attattgtag tgaccatcgc ccttcttctc cttggaacct tccttccatt
     5281 cctataaaat agtgagtgag gtgattccat gaaaatagat ataacaaatc aagttaaaga
     5341 tgaatttctt atatcattaa aaaccttgat ttcctatcct tcagtactca atgaaggaga
     5401 aaatggaaca ccttttggac aagcaatcca agatgtccta gaaaaaactt tagagatttg
     5461 tcgagacata ggtttcacta cctatcttga ccctaaaggt tattacggat atgcagaaat
     5521 cggtcaggga gcagagcttc tggccattct ctgtcatttg gatgttgttc catcaggtga
     5581 tgaagcagat tggcagacac cgccatttga agcaactatc aaagacggct gggtattcgg
     5641 acgtggtgtc caagatgata aaggcccttc gctcgcagct ctctatgcag taaaaagctt
     5701 gctggaccaa ggtattcagt tcaaaaagcg cgtacgcttt atctttggta ccgatgagga
     5761 aaccctctgg cgctgcatgg cacgctacaa taccatcgaa gaacaggcca gtatgggctt
     5821 tgcacctgac tcatcttttc ctctgaccta tgctgaaaaa gggcttctac aggtcaaact
     5881 ttatggctct ggatcggatc aactagagct tgaagtagga ggcgccttta acgttgtacc
     5941 agacaaggcc aactaccaag gtcccctcta tgaacaggtt tgtaacggtc tcaaagaagc
     6001 tggttatgat taccaatcca ctgaacaaac cgtaacggtt ctcggagtgc caaagcatgc
     6061 taaggatgct agtcaaggta tcaatgctgt catccgacta gctaccattc ttgctcctct
     6121 ccaagaacac cctgctctca gttttcttgc aacacaagca ggtcaagacg gcacaggaag
     6181 acaaatcttt ggtgatatag cagatgaacc ttctggtcac ctatccttta atgtcgcagg
     6241 tctcatgatc aatcatgaac gttctgaaat ccgtattgac attcggactc ctgtcttagc
     6301 tgacaaggaa gaactagtag agttgcttac aagatgtgca caaaactacc aactccgcta
     6361 cgaagagttt gactatctag cgcctctata cgtcgcaaaa gacagtaaac tcgttagcac
     6421 actgatgcaa atctaccaag aaaagactgg cgataacagt cctgctattt catccggtgg
     6481 tgccactttt gctcgcacca tgccaaattg tgtagccttc ggcgccttat tcccaggagc
     6541 gaagcagaca gaacatcagg caaatgaatg tgccgttcta gaagatttgt accgtgctat
     6601 ggatatttat gccgaagccg tctatcgact tgcaacttaa tcaggcaact gtttctacca
     6661 aaaaaaatcg accgattaat gaactgcacc ccaaaagtta gacagaataa atctaacttt
     6721 tggggtgttt tattatgaaa ttgaattatg aagataaagt tcagatctat gaactaagaa
     6781 agcaaggaca aagcttcaaa cagctttcaa aaagatttgt gtggatgttt ctggtctaaa
     6841 gtcatctgaa tctttgagat gagctttata aatcgctttt ttcagttttt gcactggtgt
     6901 ttcgataaac tcaaactttt tagccgtggt attgcctgat tttatagtat attgaaacta
     6961 gaatagtaca cctctacttc taaaacattt ttagaaatcg atttgactgt cctgatcgat
     7021 ttgtcctgtt cttatttcat tttactatat ttgagccact tcgtctttaa cggctttatt
     7081 cataagctct tgtaattttt ctttactatc aattacttct gattttccgt tgtaatttat
     7141 tgtaataggt tttaacttac ctaatttctc gacacgctca ttaatttgat ctttttttga
     7201 aggctgcata tgtttttcct aagatttttt cgaaaatata tttatcagat agcggtttgc
     7261 cttcttcttc agcttggttt ttgtattgat ttgaaacata aggaacaaat ccttcatagt
     7321 aacctaatgc tcccataagt tcaaaagctt gttttctaat tcaaaccatt gcaactcaga
     7381 tttcagcttt tcagataaat cctgctcatc caaataatga cttgaaatta gtgctgaact
     7441 cgtttctgta tcctgtacag gctgagcacc cataccagca aaaaataaac tcgttcctag
     7501 caagaccgaa caagctccta ttgcatattt cctcaaagaa aaacgctgct ttctctcaaa
     7561 ttgaaattct ttcatcccat ctcccatcat tcattattac tgtatatttt gtatatcaga
     7621 aatagtttgt attcacaaat ctttctagtt attcccttat cattcctaat taagggagat
     7681 aacatacaat aatttttagt taaatgtata tctatgtttt ttgtttttct taataaacgc
     7741 aatacaaaaa gagcctgttg ccaagctctt tgtactcaat gaaaatcaaa tagtaaacta
     7801 ggaaactagc cacaggttgc tcaaaacacc gttttgaggt agtagataag actgacgaag
     7861 tcagtaacat ctatacggca aggcgacgct gacgtggttt gaagagattt tcgaagagta
     7921 ttagtctatt atttcttctc agcgcgaagg gctgacaaga tttgtgttcg gatatcatcc
     7981 acaccatttg gcgtatttgg taaaaagatg gtttgatttc ctttagaggc aaaggtattc
     8041 aaggtatcca aatactggtt ggtcaagagg atagacatga tttgttcttc tgtcatgcca
     8101 acattggctt ccttgagttc ggtgatagac tctgccaatc catccacaat cgccttacgt
     8161 tgttgggcaa tccccacacc atgaaggcgg tctttttctg cttcggcttc agctgcagtg
     8221 acaattttaa tcttgtcagc ttccgccaat tcttgtgctg cgacccgctt acgttgcgcc
     8281 gcattgattt cattcatgga ttgcttaact tctgcatccg gttcgacctt ggtaatcaag
     8341 gttttcacga taatgtagcc gtaagtggtc atttcttctg ctacttggtg ttgaacttca
     8401 agggcaatct catctttttt ctcaaacaat tcatccaagg ttaattttgg aacagaagag
     8461 cgaagagcat cttcgatata agatttaatc tgagattctg gacgtatgag tttatagtaa
     8521 gcatctgtca cgctctgctc gttgacacgg tactgagtcg ctacattcat cataacgaac
     8581 acattgtcct tggtcttagt ctcaaccaca atatcacttt gcaacaagcg caactgaatc
     8641 cgtgctgcaa tcgagtcaat cccaaaaggc aagcgaatat gaataccgct attggcaacc
     8701 ttttggtatt tcccaaagcg ttcaataatc gccaccgact gctgacgaac cacataaact
     8761 gtactcagtg tgactatcac caataggagc acacaaacaa tcagaaaaat catgaaaaat
     8821 attgccataa tggaacctcc acaagtattt ttctagtatt atagcacatt taaagaaggc
     8881 tgtgccgttt ttactgcgat ttttcctgaa atgtcaataa ttagaggtga attgtcctat
     8941 tgtcgtctaa tccttgctaa aataactctt tataaaaggc aatcgtttct tctaaggttg
     9001 gcataaatgg atttcctggt gcgcaggcat caatcaaggc attcttagaa aggtattcaa
     9061 agtcgaaatc tttttcttca ataccaagtt cagtcaattt cttaggaata cctactgtct
     9121 cagaaagctt ctcaatctca gcaatcgcat aatcggcaca ctcttgatct gatttacctt
     9181 ccacatgaag gcccaaggct ttggcaacat tgcggaaagc ttctggtaca cgtttagcat
     9241 tttcacgttc tataactggt agcaacatgg cacagcaaac accatgtggc aaattatata
     9301 ctgcaccgag ttggtgagcc attgagtgaa cataaccaag accagcatta ttaaagctca
     9361 taccaccaag gaagatggca ttgaccatac cttcacgcgc ttcaatatcg tatccatttt
     9421 ctacagcacg aggaagatat tccttgataa gctcaatagc accaatagac agtttcttgg
     9481 ttacatcata agcaccaggt gttaccaaag cttcaacagc gtgagtcaga gcatccatac
     9541 cagtagcagc agtcaggcct ttaggtttgg aaagcatgag ttccgggtca ttaacagaga
     9601 taagagcaag gctattcttg tcaaccatta ccatcttaac cttgcgttct tcatcagtaa
     9661 tcacatagtt aatagtgatt tctgcagaag ttcctgccgt tgtattgata gccacaactg
     9721 gcaagccttt tttagcagac ttatgaagcc cttcataatc ctgtggtttt ccaccattta
     9781 tagccatgat agaaatacaa ctagctgcat cctgtggaga acccccgcca agactgatga
     9841 taaagtcaca gccatgttct tgcaaagcag ctaccccatc tgtgacattt ttacaagtag
     9901 ggtttggctc tacatcgcta aagatgacat attcgattcc ttctgtatcc agtggtttta
     9961 aaaccttagg caaaatatca ctgccttcga tgtacttatc tgtaaccaaa agtgcctttt
    10021 tatagccaag ttccttgata taaggaccca cttcatttac aacaccttta ccaataaggt
    10081 tgactgccgg aacataaaat gtagccatat aattttcctc ctgcgcctcc gcgctatcta
    10141 tttaacatca ttatattact ttttgtattc gttttcaatt atttgtgaat tgtttaacaa
    10201 atctttttaa aaggatagga gggacgtaag ttcacctcct atataaacaa gttttattta
    10261 tgtagtggtc ccaacaactg acatgcacga tagtctgctg attctagatc ttctgttcca
    10321 aataaggacc aatttttagg tctaaagata tcttcctcag gtacattatg catattgaca
    10381 ggaattctca acatagaagc caaggtaatc aagtctgctc caatgtgtcc atatgttatg
    10441 gctccgtgat tagctcccca attattcatg acgtcataga cagacttgaa agcacctttt
    10501 cctgtcaaac gtggagcaaa ccaagtagtt ggccatcctg gatctgtacg attatctaaa
    10561 gtatggtgaa catcttcagg aagttcaagt gtataacctt ctgcaatttg tagcactgga
    10621 ccaacccctt ttagaagatt gagacgtacc attgttactg gcatatcccc cttcgtcaag
    10681 aaacgagttg agaatcctcc tccacggaag tattcgcggt ttgctggtgg gaagtctgta
    10741 ttttcaagca tagcctgcac ttcactttct tccaactccc agaatggttt cataacaggt
    10801 ttgccatctc gagtagcttg acctgtacca tccaatgtac aagaaccaga gttgattaga
    10861 tgtaagaagc cagctgcagc acgaccctct aaagtatgtc ccgttacacg tttaacagcc
    10921 tctgggctcc aataagtacg cacatcagca aagatttgtg gagtatttgt taatagataa
    10981 ttaaagagca tagacacacc atttagtgaa tcattctctg tcgcaaatac aaatggtttt
    11041 cgaataccat tccagtcaaa ctgagtattg aggaaagttt ccataaagtc cccatttggg
    11101 aaatgatctg tccactggcg ttgaccttgg aaaccagcta ctaaagcatg gtgaccaact
    11161 gcttcttcct caaaaccaag ctcagcaagt cttgggttac caaccattaa gtcacgtcca
    11221 atcatgaaca tcttaataac aaattcccat tgtctatctt tttcttcacg gcttaaaaca
    11281 aggtcttcac ggttatggtc gaatccttct tttacgtttt ctttcaccca tttgagcgca
    11341 cgttcgaact cttcagggtc gtaaatacta cggtccatac ggcgcgtgaa ctccgtcata
    11401 tctaccgatt catttcgcat tcctaggtat tcttggaaga aatccggatt tacaatagaa
    11461 ccaccaatcc ccatcgaaac actacccatt gataggtaag cagtgtctct catcaagcca
    11521 gttgcaagag ctgcacgcgc atagcgtaaa agtttttctt tgacatcttc tggaatatct
    11581 gtgtcactag cttcctgaac atctcttcca taaatcccaa aggctggaat ccctttttga
    11641 gcatgtgaag ctagtacagc tgcaagatag acagctcctg ggcgttctgt cccattaaat
    11701 ccccaaatag catgaggaat atctggagac atatccatag tttcactacc ataacaccag
    11761 catggtgtaa ctgtaattgt tgcgcaaaca tttgattttt taaacaactc atgggaagct
    11821 gcagcctctg gtacacggcc aatagtagat ggagaaatca cgcattccac aggttcccca
    11881 tctggatatt tcaatgtgct tgaaatcaaa tctgccacac ttttagccat gttcattgtt
    11941 tgcacttcaa gtgattcgcg tacaccttga cgacgaccat caatagtcgg acgaatccca
    12001 atacgtggat gttgaatcat actattttcc tcttattttt ctgtttcttt catactacca
    12061 tctccattat atttacggta gcctgggtga cgacctgtaa ccttataatt ttctcgcatt
    12121 gagaatagac gttctaaagt cggacgagca atttcttgct cctcaatgcc ctttgtagaa
    12181 agtaacattc ttccgtggaa ggttgtcttt gcgactaatt ctaaagtttc catacggtag
    12241 tatgctgtaa ttacatcgct tccgacagtc agagctccat gattttctaa tagcatgaca
    12301 tcatgatcgg gcagataagg tgtaattgct tctggcactt ccattgtaga cggaactcca
    12361 aatggcgtta taggaattgc ccctaccaca atcgcgctct caattagtga ataagtatct
    12421 aaaggaatgt gtgcaagagc aaatcctgtt gcaatcggtg gatgcgcgtg aacaactgaa
    12481 cgaacatcct cacgttcttc gtagcaccga atgtgcattt taatttcact agaaggacag
    12541 taatcacctt ctgcttctaa aatctctcct ttaagattta acttcaccag cttttctggt
    12601 gtaataaaac ttttgctgat accagtaggt gttgcaagaa tggtatcctc atctaatcga
    12661 acagatacat tcccatcgtt agcagcaacc caaccaagtt gccacatctt atgacaaaca
    12721 tcacaaatct gttcacgaat taatttttca tcttgagaca taaactcact cctttctaaa
    12781 aatgaagttc gaattcaaga tttccgttat tgttaatggt aatagtaacc atatgatttt
    12841 tctgatcaaa attgagagat ggctgatcat aggaattatt tgttcctgat aatgaatgaa
    12901 tagtaggaag tttatctata ccccttaata ctatctttgt aacccttttc tctccaaact
    12961 gagtatcttt gatataatgt tcttcaatcc acttaatggc acctttcttg gtcaattgtg
    13021 gtagtttctt tgcctgattt gcatcttgag cattagacca tagtgaatct ttatttgttc
    13081 taaaattatt taaattcact ttcaatccat tacttattct ctcaaaaata ccataggtat
    13141 gaggttcaaa ttcaacatcc atttctaaag aattaaattt taatttccca gtctgtttaa
    13201 cattctcatt taccttataa ttatagacaa accatctatt atctaatttc tgagcaaaga
    13261 tatctccttc ataattcata ggatacagct tatttaagta ttcttttcta gcctgagtag
    13321 agctaaattc tggagaagtg atttccttaa tctgaatacg gctaccagaa acagactctt
    13381 tcaatttatc tgtttttaaa acacctggaa tagctggaat aacattataa cgaccagtca
    13441 tgtataatgg agtttgttct ctatcagtat ttacgtttac aaagaactta ccatttcctt
    13501 tatttgaata gtctccatgt atgaatactt ttgtatcctc aagtaccttt tctttggatg
    13561 gtgctggatg cgctattaca tatctaaaaa attcttttat tacttcgcta aagagtaagc
    13621 tttctttatt atttacacca taggtataag ctgggtgttc aaaattataa actacacctc
    13681 cattaaggta aacccctaaa gcttcttctc ctagcataga ctctggttca gtcaaccatt
    13741 gtctatctcc ttgacttttt ccgatattac cacttgcaaa cagtttccat ttacctgtct
    13801 cataccactt ccaagtatcc atcaaaccac cccattgata agtatgattt gaaagccaaa
    13861 gtcccttcat ataactttct gtagttgaat catttccctc agcagcaggt gtatttttga
    13921 acatgaaaat aaggttcttc cagtacttat caacagattt ctgaaatgct atcttaccgt
    13981 tttttccaaa tgctttttct attgcagaac cattattttg ttcagcccat ataaaatatc
    14041 ctccattttt agctgatact tttaagtaat ctgccgcctt attttcaata tcatttgccc
    14101 aaatccaata attctcagta ctgaaaatac catgcaaatt tggatatttt tgatacattt
    14161 tatcaatcca gctggtactt aaccaatgtg ccgaagtata ataaggcata ttaccagcag
    14221 tgtaaaccgt tagaataact ggaataggtt cattttttcc agtcttagga tttacatatt
    14281 tcgctgcttc ttctaacata tgctcataaa aatctctagc agctccatct cttttaggaa
    14341 gatcatctgg atgtaactgt ataacagtat atggttttaa gtcatctggt atattctccc
    14401 acttacctgt taatgtatct cctccccaaa aagtatattt cccttggtta aactcttctc
    14461 cgttagcata taaaggcatc ataagtaacg gatgttcatt attaatttca cgacgtttat
    14521 caacttctga ccaatcatta ggattcttaa atttaccatt tgccataaac aaggctccac
    14581 ctaaaaccaa ttcgtcagcc catgtatgtt tccctgaata tgatttaagt tctattgtct
    14641 gtgtatcttt gggaatttca acatcaacca gaaatgccga tgtatcatac ttaattcctt
    14701 ttggatattt tgaatctgat gagtaaatga ctttaccatc cgcaacaact tcaaattttt
    14761 gaatatctgc gtaatctgaa tttaaatgat ttgcactcct atcaattccg acataggaaa
    14821 taaatcttgt aacattagca cccgaaacat catattttat actagacggg ttacttgcta
    14881 cagttcccaa tcccttatta aattcgactt ccttaccgtc aattagtaat tttattttat
    14941 tattagaccc gttattacca ggtgtaaacg gtttatcttt ctgaactgtt tttgatttat
    15001 cgtcatcacc atgagtagca tcaacccatt ctaaatcaga aagatatact cctccattaa
    15061 ttcctttatt aagaagatct ggagttatag tttgagtact aacagtttta ataagtttag
    15121 catcagcaaa tacaacctca tcaccccatg tatgttctcc tgcgaatgat tttaaggata
    15181 tttttttagc gttttgtgga attgtcacac taataaattg tgcctgggta ttatatctga
    15241 atccttctgg attagttaca ctactagaat agacaacctt tccatcaatt tctatttcta
    15301 ttctatccac tactgcatgg tctgatctag aactattagc actttgatca attcctatat
    15361 aagtttcaaa tttttcaaac ccttgaccgc tgatatcata gctgattact gatggagaat
    15421 ctgcgactgt tcctattcct ttatcaaaat atttaacttt atcatcagaa gtgagcaatg
    15481 aaatcttagt atgctctcct ttatttcctg ttgtaaatgg agcatctttt tgtactgttt
    15541 ttgttttatc tatatcacca tgagttgcag aagaccaatc catatctgaa agatatgtca
    15601 tctctttatc aatttcttta tctttagtaa taagtttttc taagttattc cctgttactc
    15661 tagcagtttc agtactagct ctgttattag aattactttg aactacatct gcataaacca
    15721 aattacttcc taaaaaactt gttaaaacag ccgtagctat taaaatctgt tttgctattt
    15781 ttttactttc catttttatt tcctcaatat ttacatacaa cttcttcaat aacaaataat
    15841 tttgtaagat attctggaac acaaagtgct aaattactta aacttacttt tattccagca
    15901 ttcattaatt catctccata aaagaactca tcaaatccag agacggaata gcatttatct
    15961 ttatctaaac cactaaaata aactcttgta taacctggat tcaacttact attcaattga
    16021 gcatacaata aaatcgattg gcatttatct tttgaaacaa cttgccaact agcagaatca
    16081 caactaaaag gactgtttat cctatagaaa tccccatatt gaagcaatga acgatatttt
    16141 ttataaaact gaatttgttc tctcacttgt tctttttctt ctaccgacaa tctcgtaaca
    16201 tctaattcat aaccgaaaga actaaaataa gctacatttg tcctagtgtc caaactcgtt
    16261 tgtcttccac tttgttcatt tggagaaata gaaacatggg ctgtcatcat tgatggagaa
    16321 tatccataag aagttccatg ctgaattttc aatctttcta tcgggtccgt atcatcactc
    16381 gtccacgctt gcggtgcata atacataagt cctaaatcaa aacgtccacc acctgaagaa
    16441 caagattcaa atagaactga agggaactta gttattaaac gatctaataa ctgataaaga
    16501 cctaagatat atcgatgacc aaattccatc tgctgttcag aatcaagttc aatactataa
    16561 atatctgtta tatgacgatt catatcccat ttgatataat ccagatttgt tttgattatg
    16621 attttagaaa tagaatcaaa aagataatca acaacttcct gtctcgtcaa atccaacaca
    16681 tattgatttc ttcctactga cttctctctg tctaacaaat ggatagtcca atcggcatga
    16741 ttcttgtaca aatcactatc aatagaaatc atttcaggct caaaccacaa accaaattgt
    16801 aaacctattt tgtgaatttc atctgcaaga aatccaatac cttcaggaag gcgacttcta
    16861 tctgttaccc aatcacccag agaacttttg tcatctttcc tatgaccaaa ccaaccatca
    16921 tccagtacaa ataattctat ccctaaatct ttagcagcct ttgctaaatc taaaattttt
    16981 tctgtactca agtcaaaata aaatgtttcc caactattga gtattatagg acgactagct
    17041 tgagaaaaat taggattaac taaatgatgt cgataaaaat ctgatagttg ttggctcatc
    17101 tgattcatcc cttgatcaga atagatcaca attgcttctg gtgtcacaaa actttcggca
    17161 ggatttaata aaaaacgaaa ttctactgga tttattccta ccgttactct agcggtgtca
    17221 aaatggtcaa cttcaatcat attttgaaaa tttccagaat agataaagtt aaaaccataa
    17281 acctcaccac tatgctctgt agtttccctc cttgatagca taaagaaagg attttgctga
    17341 ggacttgatg agtatctcaa gctatctatt gcttgaatac ctggtctaag cgaagttcta
    17401 tataactgac gttccctcag ccatgctcca gaaaattgaa taaagtcata ttgactatca
    17461 ggtagatcta gctgtaagct cattgctttc aaaagagtca acttattatt gctattattt
    17521 aaaaattccg tatttctaac taagacattg tattccggaa aaattgtgta atttagtcta
    17581 acagttaaat ctaatatgga atctgttaaa ataatcgtta atgtttccgc acactgacca
    17641 atatcatcaa atgttgaagg tagagagtta agacgatttt tccctttttc aagttcaaaa
    17701 ccttgatatt ttagtgtcgt tatcctacta ccattcattc cttgaacttg aaaggctgga
    17761 tgccgaaaat ctcctttccc atatgaagaa tattcatttt gtatcataga ctgttgaata
    17821 tcctcaaatt cttcagaaaa agatacttca aatcctcttt tttcataggc catcaattgg
    17881 ttataatttt ccttgacatg aatccttttc ccaaaatgta gatgttgaag ttccccattt
    17941 tctaaaattc taaaaatata actactatct ttcattgcca aatgaaaaac ggtctcgtcc
    18001 ttattaatat aaatcgtcat ctctacctca caattttttc tttacttctt cagtatcacg
    18061 atagagtacg gtgccaattc tatctcatct tcttgaatag taaaatctcc aattactctg
    18121 gtaaaattct ctatcgaaag attctctatt tttatttttt gtttaatctg agcaggattt
    18181 ctcagagaga gaatttcagt agaagtcttc tgattgtgac acttataacc ataaatttca
    18241 aatttttcag gacttcctcc tattttttta ctatatttta gtgtttgata attctcctct
    18301 atccatttga cggcacaagc attggctttc cagcgttctt catcaaacat atcaaaagaa
    18361 tagtgaaact cccaaaaagc attccctctt gttgaaataa ataatagata agcttcaaaa
    18421 tctggtattg atgcaaacat ttgatgatcc atgtaccagg tatttgcact gacagcatag
    18481 attggttcat gattataaag cgaccacata ggtaactgta tctcacgttt ttccaaaaat
    18541 tcttgatact gactatctcg gtaagtaatc atacgattga tatcattacc tgcattctct
    18601 gtaaagccta catcttgaga tatttgaatc cataaactat tgacccactg taaaaaccat
    18661 ggactaggat ttacataaga agtcaagttt aaccaacaat cttttcctcc tctctccttt
    18721 cttagatcta tcaacagttg aattaagaac tcataaaccg ctgtcatggt atacataccg
    18781 tgcggtccac tcttatcagg tttgtcaggt tgaagtaacc aaccatcaat tttccaatag
    18841 ctgatatcga attctttttg atattccaac atctttttct tcatttgatt gagatagtta
    18901 aaatcagcca cgtttacatc atttgaaatc aaatttttag atccaatatt taactctgga
    18961 tgtgcttcta accaatcact cataatgatt tctgtcccat tataaccacc tcggggacca
    19021 atccacaatc ctaggcttga accaaatcca tttacaagat gtttaatatt tctcaaacca
    19081 tttgggaatt tatgattgaa ttcccaaact gattgatagt ttgtccaacc atcatcaaca
    19141 acataagcat ctaaatgaac tccatgattt tcaaatccat ctcgaatctc agaaaaactt
    19201 tttaaaatac cttcctctgt aatatcggtc atatggtcat accaggaatt atactgttta
    19261 cggaaataac taggttgagc tattccttca acatattcaa aaaatgcctc ttgaatctct
    19321 tcttttttat aagaacatgc tcccccaata atacaagacc acaaactctt ttcttgattt
    19381 acaacagttc ccaagtaata tcttgataca tagtgtctac catctacctt gttttcagac
    19441 attggaaatt ccattcctaa gaataaagaa ttcgcataaa caggttgtcc caattctaca
    19501 taataaccgg aaaaattcgc catttcctta atacaatctt gtcttttcgg atagaaaata
    19561 ttgtcttctg tttcaaactc aaaactctct acctctacag atttaataac ctcagtagat
    19621 gattctaaaa ctaattgctt cgaaagaaca tcatccctcg attcaaagat taaacgtaaa
    19681 cgaatggaag gacaaacaaa agaaacctga attcgcgtat ctccctcttc cctaacgatt
    19741 gctgaaaaat gatttgaaga aagtcttata ccattctcca atgtaatggt aaattcttct
    19801 ttcgaaacat tttttatcat tactcctgcc cgtttgttta cgatatcagt agtataaaat
    19861 cgaccctctc cccaaaagaa attacgtctt acatttttat tttcaatttt catataaact
    19921 actctctcaa ctcaattttg attacgctat caatcaagtc tggtaatgga taggtaaaat
    19981 gtggaacttc tccaaactgt gcaaaacaaa ttcctttgta ggcattggtc gtccagcttt
    20041 ctgaaatttt cacctcactt ccatcatgaa gaaagctcat tctttttacg ttttctttac
    20101 taataccaag aagagctaaa ggacctatag gttgttcaaa tacatgagca taaaccgtat
    20161 tttcattacg agtatagtat ccccaatctg gttttggtaa attttcatct ccactacatc
    20221 cataaattgc ctcaccattt cgagacatcc atcgatgaaa attatctaat attttcttac
    20281 ttgaatcatt aattcttccc aaagcatctg ggcctacatt aagaatcata tttccgtttt
    20341 tactaacaca ttccactaac tttcttatca aagtttgact agacttatat agataatctg
    20401 taggattata agcccaattg ttattcatag ttagacaaag ttcccaagga acaggttcac
    20461 ctttaaagtt tcgaatacct tcgtgaggaa caatttgctc tggactaaca aaatctccag
    20521 cgtaagaggt aatttcatct gtaacgatac ttccgaatcc ttcacctgat gtttctaatc
    20581 tattatctac gataacgttt ggctgataat gacgaaccat gtctattaat tttgaagctc
    20641 cccatttttc acccaccatg tcttcataag aataatcaaa ccataatatg tcaatctgac
    20701 cataaccagt tacaatttct ttaacttgat tgtgtaaata ctccaaataa ctgtcaaagt
    20761 taattttctc atctcgatag gcttcatttc ctctcattgg atgatttaga tcagcatatt
    20821 tagggaaatc aggatggtgc cagtcaatca aactaaaata taagcctact tttaaacctt
    20881 cagctcgaac agcatctaca aattctttaa ctaaatctct acctgcaggt gtatttgttg
    20941 ctttataatc tgtaaatttt gaatcaaaca aacaaaaccc atcatgatgt ttagccgtta
    21001 aaaccatata cttcatccct gcagcttttg cttgtttagc ccattctcta gggttatatt
    21061 ccttaggatc aaaagctcga aaatatggtt cataatcttc aatagataat ttctgatgac
    21121 tacgaatcca ttcccctttt cctggaatag aatataaccc ccaatgaatg aacatcccaa
    21181 atcttgcttc tctaaaccac tgagttctac tattgatagt ttcaagtgta tgtttcatcc
    21241 ttttactgct cctcctccta aagcttcaat gaagtattta cttgtaaatg cgaatagaat
    21301 gattggtggt aatacgatta caacaatagc tgaaaataga ccattataat tagtattaaa
    21361 acttgtagta aattgaccta gtaatgctgg tactgttaat tcatttttat caatcaccaa
    21421 catagatgcc caataatatt cattccagtt attaataaag gcaagtactg ctgctgttaa
    21481 aattcctggt ctagcaattg gtaacatcac tttccaaaaa gtttgagcat atgtcgcacc
    21541 atcaatctct gctgcctcct ccaattcttt tggaataatt tcaaaatagt tctttataat
    21601 aaagaaactc atagcaattc cactacaaga gtagaccaga attagagcaa ctggggtatt
    21661 ataaatcccc aactcattta ctaatctata aactggaaaa gtcatagctg ttgctggaat
    21721 aaatagagta gaatacagca tagaagtcac caacttcttc cctctaaatt ccatcctagc
    21781 aataacatat gaagacatgg agatcataac aatgctaata ataactgata tagacactac
    21841 tttaaagcta ttccaaaagt aagtgaaaac atgaagtttt gtaaaaacat ttatatagcc
    21901 ttcaagagta aagtcagtcg gtaaactaat acctggattt ttcataggat ctgcttttaa
    21961 tgatgataaa aaaatccacg caatagggaa aatagaaatt aaaatggtaa ggagcatata
    22021 accatatata agaactttgc cttttttact aataccattt atatgtgtat tcataatcat
    22081 ttcccacctt tcagtctttt tctttctctg aataaaatat taatacaaaa gataatcaac
    22141 attccaccta aaatttgtat aactccaatt gtatttgcac gagcatactg agttcgtgag
    22201 ctagccactg ctaattctcg aataacaaaa ctgatacttt ttgttcctgc agcaccattt
    22261 gtcaaaaaga atacttcgtt atagagcaaa aaccctgaag ttgctgccat aattgaaaca
    22321 gttttaattg tccccttaat catcggaatc gtaatatacc actctttcct ccaaccagta
    22381 atattgtcta aaatagcagc ttcttgaact tcttctggaa tagcaaaaat ttgcccaaga
    22441 atcataatgg ttgatgctcc tgcaaagaag atataagcgc cagtcatagc tactatattt
    22501 aatcctggag tcaacagtac tgaatcttta aacgatggat taaatagatg aataatttgg
    22561 ttaacaacac catagtttgg attatagatt tgaagaaaaa tcaagcccat agcagcacta
    22621 gaaatgatac ttggtacaat ataaagattc ctagatatct tccagccttt taatttctta
    22681 gataatgtaa ttgcaacaga tacagctaaa ggaacttgaa taataacacc aattacagcc
    22741 caacgaacag aattaattag agcttctgta aaaaatgggt attctttaaa aacataccta
    22801 taattttcaa ataatttatc aaatccaaga aactcagggg tgtttaaatt tgtataatcc
    22861 catttagcaa atgaagttaa aaagattgtt actacagaat aaaaataaaa tagaaagaaa
    22921 caaatcaacg ttggcaaaag aaataaaaag ataaatcgtc ttttttgatt cttcctattt
    22981 tgtaggctca taattttttg tgccataaac aaactccttt tttcaactat ataagtaaaa
    23041 tcaagctcag ttggcagtgc caactgagct tcgttttcac tccctaaaac aattaaccaa
    23101 tcaatgattt caacacatct tgagtagatt ttactttttg atcaacatct acattttcag
    23161 cagcactttc tgtcaatgca ttgataatag ctgtgtgtac atcgccaccc caaacatcac
    23221 ttacagttgg aacaactgct tttgcattct taacttgagt gattgcttgt cctaaaattt
    23281 tagtagtagc ttctgaactc ttttctgaaa gttcttttac atttacattt tcattagaag
    23341 gatttgctcc tactttttca aaaattactt tttgtacatc atcgctagtc atatatttca
    23401 aaaactcaag agctagtttt tgtttagctt cagacatctt acttgagata gtgataccac
    23461 ctccagaaga actaattgct acacctgcag gatagattcc aggtgcaaga cttgggttct
    23521 tagacatctc accagaagcc cacacaccat taaagaatac tgcagattta ccttcttgga
    23581 aatctttgga ataagcattg gcatcgccac cagcattctt agaaccatta gcttgaattt
    23641 ctttcataac cattttcaaa gcatctgcaa attctttaga ttcaattcct tcttttgtta
    23701 atggtttatc taataattta cgtccatttt ctgtggttcc taatactgta ttaaacaagc
    23761 gaatagatgg ttctccagca ccaaatgcat acactccatc ttgttttcta atgcttgcca
    23821 ttgcctgagt gaaatcatcc caagtattcc actgatcagg tgtcttagca cccgcttttg
    23881 caaatatgtc cttgttgtac cacaaaccca ttgtaaataa ctgttcgtga actgtataaa
    23941 tttttccatc tttttggttt tgcttatagt tcaaaccaac atttttttca agcttatttg
    24001 aatcgatata tggtttcaaa tctaaaacaa gtttttgttc aatcgcagct aatgatactg
    24061 cattacctgc taggtcaatc acatcaggaa attcaccact agcaaccttg ttattcatta
    24121 tatccttcaa atcaccattt acaggagttg gtttaaattc tacaccacta tctttatgtt
    24181 cttctgcaaa tttatcatat aaatctcgca tagtcttcgc tacaggccat tcatcttcac
    24241 tatgatgata cccatgataa aattctaata cttctttacc agaagaactt ccaccgcttg
    24301 catccttaga tgttccacaa gctgctaaac caaaggtagc tactaacccg ataacagcgc
    24361 atttcaaaag actttttttg ttcatgacaa aacctccgtt tttttatttt tgtttatgat
    24421 agctgaggat aataatattg tgtatcagca atatttgtca taatatcctt taactctgta
    24481 tcctcgttta ttttaccttg actcaatagc tgcacaacaa tattccccac agctgtaccc
    24541 tcagtcgaac ctgtaagaac ctctttacca gttctatcag caatcatttg gttaaagtaa
    24601 ctggctctag cacctcctcc aatcacatat atcctcttat aaaccttatg agttagttct
    24661 tctagtaact ctatcgcttt cctatacgtt tcagctaggc tttcataaac aatcttaaat
    24721 agttgtccat ctgtccactc tctagtttca tgatgataag ctagatattc tgtcaaagtc
    24781 ttgtgcatat cagattctgt tgcaaattca gttgattcag tatcaatcag aggaagattt
    24841 tctttttctt tctccaccat tgtcctaata tcatcaaaag aataggcttt ccctcttcgt
    24901 tcaaatgaac gtcttagttc ctctatgatc cacaaccctg tacaattctt cagaaatgta
    24961 atcactccat ctttaccgac ttcatttgta aatccataac tgaaggattc ggtagtaaga
    25021 atcggtgaag taagttccac tccaaccaaa gaccaagtac ctgatgaaat aaataaacta
    25081 ccttctgtct taggtactga gacaatcgca cttgctgtat catgactaca aacattcaca
    25141 acaggaatat cgcctaaacc atactccttt tttatccttc caagaacatt tccctctgaa
    25201 acaatttcag gaagtaaaga tgaatccaat tcaaatagtt ttaagatatt ctgattccaa
    25261 ttttgactcc taggatcaaa taattgagtt gttgaagcaa tgcttttttc tgtagcaaac
    25321 ttacctgtca agagataatt aaacaaatct ggcattaaaa gaatcttatt ggtcttatag
    25381 aaagagtcag gagattcttg acgtgcctta aagagttgaa acaaggtatt tatctccata
    25441 atctgatttc ctgtctctga atacagtttt tctaattcag tcatttcaga tatttccttt
    25501 aacactccct ttgttctttc atcacgataa tgaacaggtt gtaataacag cttaccttca
    25561 ttatcaatca gtccaaaatc aactccccat gtgtcaatac cgatagataa aatcttgtaa
    25621 ctagtattag ccaagcggat actttcaaga attttagcta gtagaaagtc aatatcccaa
    25681 gataaatgcc cttttactct aataggtaga ttagaaaagc gatttatttc ttccattact
    25741 agtttatttt cagaaaggta accaacgatt gctcttccag aagtcgcacc taaatctatt
    25801 gctaaaaccg cttccatttt tcttctctct tcgtccttga ttaactttat tataatcccc
    25861 acgttttatt ttttcaatat gtgaacgctt acttgaataa tgttcaaatg ttaacccaat
    25921 attcataaaa ataattcaaa ttcctccaat ttgtgctata atatagttga aatgataaat
    25981 taggaggagc ttatgataaa agatgaacgt gtacttgaat tgattgaaat tatcaaaaag
    26041 aaaaaaagaa ttgccgtaaa agagctggca gaaatcactt tctccagcac aagtacctta
    26101 cgtcgcgatt taattttctt agaaaatcaa ggtcttatca aaagaaagca cggatacgtg
    26161 accctgtcct ctatgaacac aattgaactt tctcatcaaa tacgggaagg agaaagtact
    26221 aggcaaaaaa gactaatcgc tagtctcgct aaagacttta ttcggtctgg tatgtgtgtt
    26281 tatctggatt ccagtactac tgtctacgaa ctttgtccct atctttctga acttgataat
    26341 ttgattattt ttacaaatgg tttacatact gcacaaaccc tatctgaaac tgttaaagat
    26401 agctccaaaa tctttatcac atctggcgag gtcaaacatc aatcctgttc cgtggtcaac
    26461 tatgataagg aaaattcttt attagatcat tttaatatcg atttagcatt ttgttcagca
    26521 agaggtattg atgaccaata tgtttatgaa gcttctctca gccaagctat ttcaaaaaag
    26581 aatattattg acaaagccca tgaaaccatc ttactgattg atagttctaa attttacaag
    26641 actggatttt ttaaaattaa tcccctctcc aaatacacaa cctttatttc agacaccgtg
    26701 ccagatcaaa aattattaga cgcagtcgaa ttatttgatg gagaatgggt ttctgatatt
    26761 caatgaaaat caaataggaa gctagtctca gcttgctcaa agtacagatt tgaaaactga
    26821 tgaagtcagc tcgaaacact gttttgaggt tgtagataga actgatgaag tcagtcacat
    26881 atatacggaa agacgaagct gacatgatgt gaagagattt tcgaagagta ttcgttctgt
    26941 ctgctgactg atttacaaat attcaccaca tgacattatt ttctcagcaa ccctaaaaac
    27001 agaggctgga caaaaactca atttcaggag aaaatggagt aaaccttccc acaataaaac
    27061 gcatagtatc aagtttttgc acacctgata ttatgcgttt ttctgatttt aaagactttg
    27121 tcgcctagac cctatatttc aagaattaag tgaccttgat gatctaaatg tattgtggta
    27181 tactattttt gtcatcggtt accgattacg ttcctatggg ttcgtgagag gaggtgaaac
    27241 taatgagcct tatcatagag ctcgctctta ctattatagc ggatgttata gctggaatta
    27301 tcttgtattt cgtctgcaga tggctagata gtaagaagta gaccgagtga ctagcctata
    27361 aacacccgtt aaatcgctaa gatacgtcaa aaaagccctt aactacggcc atagttaggg
    27421 gctttggtgt tctaatgaac cttatcaatt aactacattt tagcatataa tctacaatag
    27481 ttcaagaatt aagtgacctt gatgatctaa atgtattgtg gtatactatt tttgtcatcg
    27541 gttaccgatt acgttcctat gggttcgtga gaggaggtga aactaatgag ccttatacca
    27601 gagcttgctc ttactattat agcagatgtt atagctggaa ttatcttgta tttcgtctgc
    27661 aaatggctag atggtaagaa gtagaccgaa tgactagcct ataaacaccc gttaaatcgc
    27721 taagatacgt caaaaaagcc cttaactatg gcactagtta ggggctttgg tgttctaatg
    27781 aaccttatac actaactaca ttctagcata taagcccaga tatttcaaga gttttattta
    27841 ttttttcagg ttcccttagt tctgaaaggt ctataatgaa gttagccatc tagtatcaaa
    27901 aaaccgacta gctcttatga actagtcgat ttctcatcaa tgcgccaaca tttcttgggc
    27961 gatttcttgg ccagataggt tatctgggta gtaggttggc caattatcca tttcttcaaa
    28021 tagagtttct tggcttgtac ctccaaaaaa gatatggaaa tgttctgcct taactggggc
    28081 gatattgtgg tcactaaact gaacatactt gaattgtcca gcgtcagcat ctgtggcttc
    28141 aaagaggaaa cgcacgccac gattgccttt cttgtaagtc aaaattttct taccgacata
    28201 cttgtaagtg tatttcttgc tttgtccacc ttgaacaaat tccatagtat tatcagtaat
    28261 gttaatctta gtcacatctg tctgatagcc ttttgtatag taagccttgt actcagcctg
    28321 ggtcatctta ccagtcaact tagccttgta gtcaaagact tggtcaaacg tgccgtcttc
    28381 aaggaaagga taaactgatt gccagttacc tgcatagtca ctcaaggtgc ggtccttgac
    28441 agctgcatcc tcgaagtaac cattttggac tgtcttggta tcctctgcct tttcaggttc
    28501 gattgctggg ccttcttggt ctgttgtttg tttcaaagcc ttgaggtttt tctccatcac
    28561 ggaaatgtag ttttctccag ccttggtgtc ctcttctgtc agactttcta aaggattgag
    28621 gacatcagtt ttgacacctg cttcttttga aagtgtgtta gcaagggctt gtgaggcatt
    28681 ttcttcaaaa tagatatagg cgattttatt tttcttgaca tactctgtca attctgccaa
    28741 gcgagcagct gatggctctg catctggaga aaggcctgag attgcgactt gtttgagtcc
    28801 atagtccaag gcaagatagt taaaggctgc gtgttgagtc acaaagctct tttgttttgc
    28861 ttgagacaag ccttctgcgt aagccttatc caaggcttgc aatttttcga tataggcagc
    28921 tgcattcttc tcaaaggtct cttttttatc aggataatct gctgacaagc tgtcgcggat
    28981 gtgctctact agtttaatgg cacgaactgg tgataaccaa acatgggggt caaactcatg
    29041 gtgatgacct tcttctccat ggtcatggtc tccctcttct tcctcgccac ctggcaagag
    29101 caacatatcg cctgtcgccc tgatggtttt cacttttttc ttatccaagg tatctagcaa
    29161 tttaggtacc catgtttcca tgttttcatt ttcataaacg aaggtatctg catcttggat
    29221 tttggcaact gccttggcag atggttcgta ttcatgaggt tctgtcccag caccgattag
    29281 gagttctaca ttagccgtat ctcctgcgac ttgcttggta aattcataga cagggtaaaa
    29341 ggttgtcacg atattgagtt taccatctgc ctgtttttga ttggaacaag ccactaaaaa
    29401 caaggcacat agactggcta gtaataagct aatttttttc acgttcgtct cctatttgat
    29461 aaaacgtctt actaaactga ttagtataaa gacagttaca aaaataatgg taatacttgc
    29521 acttgcaggt gtttctgcat agtaggaaat gtaaagtcct gctaccattc ccaaaaagcc
    29581 aatcgcactg gcaagcagca taaccgattt aaagtttttc cccagacgca gggcaatact
    29641 agctggcaag accataatgg tcgataccag aagagctcct gctgcaggaa tcataagggc
    29701 aatagccacc cctgtcacca tgttaaaaag aatggacatg gtacgaactg gcaagccatc
    29761 cacaaaggcc gtatcttcgt caaaagttaa gatatacata ggacgaagaa agagaaaggt
    29821 caaaatcaaa acaaccgccg caatgacaaa gagggaaatg acctgttctt cactgatagt
    29881 cacgatcgaa ccaaagagat attggtccaa actcattgaa ctcgagcttt tacccttgct
    29941 catgacaatc agagaaacag ccagacctgt tgacatgagg atagctgtcc cgatttccat
    30001 aaagctcttg taaaccgtac ggagatactc cagaaagacc gccgcaatca agacaatggc
    30061 aatagtagaa acagttggag aaatccccaa aaccagacca aaggctacac ctgaaagtga
    30121 gacgtggcta agggtatcac tcatcaaact ctgacgacgc aagatgagga aggttcccaa
    30181 taccggtgag aaaagactca tagcaataac cgccaaaaag gcgcgttgta taaagtcgta
    30241 agataataaa ctaagcatgg cccacctcct ggccattctc atgaacattg aaacaacgcc
    30301 atggcgagtc ttggttacgg actagatgaa tattgcgatc cgcataatcc ttaacttctt
    30361 cagggtcatg ggtaatcatc aaaacagcct tgccatgatg atgggcactg tgatgcatga
    30421 gttcgtaaaa ttcattttta cttcctgcat ccatccccgt tgtcggctcg tctaggataa
    30481 acacatcagg gtcagaagca aacatacgcg caatcaccgc tcgctgcttt tgtcccccag
    30541 atagagaacc caagcgtttg tctcgatgtt cccacatgcc aactgagtcc agactggcct
    30601 tgatatgctc ctcatcatga gcattcaaac gacggaacca gccttttctc ggataacgac
    30661 ccgacttgac aaattcatag accgtacttg gaaaaccagc attaaaactg gcaatttgtt
    30721 gaggaagata ggctattctc aatttcttac cttgcgtatt tgtctttgaa atagccacct
    30781 ttccaatgcg tggttgcaga attccaagac tggccttgat gagcgtcgtc ttagccgctc
    30841 cattttcccc agtcaaggta acaaattccc cactatcaac actataattg atatgttcaa
    30901 gaacaggctc cttatcataa tagaaggaca aatcctctac cgtaatatat ctcattattt
    30961 gatttctcct actaaagcag tcaaaaaccg ctgaatcact ttttgttcat ttggagtaaa
    31021 ctgagtcgcc acttgttcat aggttaaaag tgtatgctca tggtgatggt ggtgctcctc
    31081 agcgattgga cgagccaagt cagtcaactg ataaaaaatc acacgcgcat ctttagaatc
    31141 tttagatgtt tccaacatcc cttccttgac caaagactta atggccttgg taactgccgc
    31201 ctgactgaca ttgagacgac gggccaattc tgaatttgtt aaagattcct ctgacaagag
    31261 cataaggata tgctcctgag tattggtcag ggccacctcg ctagtgcaat gacctattag
    31321 gatttcatgc tgattttccg cctgcaaaat cacctcattc aaaaaagcat tgatatcctt
    31381 tgctagctgt ctcatatctg actcctttcc ttttagactt ctctttttta agagaaaaat
    31441 actattcttt gacattttgt ttaccagtta attatatcac aagcaaaaaa agagtcaaga
    31501 aaaaacgtga aaactagttt cattcttgaa ctcttctata ttatattatc tattgaaatt
    31561 ctttgacatc tccatcataa gtcgcccaat ctttgctgaa aaagcgctca ttcagatggt
    31621 aagtcggagc tggtgtggga ttggatagga aaggatcaac tgccttgtca aaagccaacc
    31681 aacccaacca accaaggtga atggtgtcct tcataaagaa aggctccccg ccgtccttag
    31741 aaaaatctgc tatattggta aaaccttgac tttctaactg gtagcgaatc ttctgcaccg
    31801 tttgttggta catatcctct cgtagaccag catagtccat ccatttttta ttaacaggtg
    31861 gaatgataaa aatcgggttt accttagatt tagaaaactg tgttaaaacc aactgcaagt
    31921 cattatactc tggcgacttg agataggtaa agcttttctg agaatccttt aatttcttca
    31981 aatccttctt gatctgctca ttatagaaat aattttccat tcccatctca ttattggaag
    32041 tatttttttc agcatctgct ttgacaacat cttctattgc ttgataagaa aactggtctg
    32101 gcaagatttt taaatactta gctacatgct tatcgtagtt aacatagcct ctaaccgaaa
    32161 actgaccaaa aaaggaggct tggcgttcat taaaacgagc caataattca atcatttcat
    32221 tgtctgctgt cgacaattct tctttacttg ccaacttctg aaccagatcc ttcatagcta
    32281 cgtttgggaa ctgttgcagt aagcgagtcg ctgcatattg actagcctga tccccagatt
    32341 gatgtttcag aaaactagtc aactggtctc cattaaaata ctgctggaag gctgctggat
    32401 catagccatt tttactgaac cactgaggtg agataacata cacaacttgt ttattctcca
    32461 gctgtggtaa catctgttgc attccaaaat attggttaag cgatgcagct cccccctgtc
    32521 ctaaaagata aggacggtag gaacgattgt atttctcagc taataccgca ggatgagcac
    32581 cgtcaaaacg aagccattca ctagagccaa agaagggaac aaaacgcaca tttggatcag
    32641 atagtgctct gactttttga cttcgctcct taaaactatc gatagtagta gccactgctg
    32701 aacgcttttc agctcctaga ttatgatgca tctcagtagg ataaaagaaa atgagcagaa
    32761 aaaccaacaa accagcgatc aagaccggtc cgaagatcat ccataagcgt ttaagcattt
    32821 tgtagctcca caataccagc tatgatttta ttagctgtat tccagtcgtc acgaccaaac
    32881 tctgttacag ggacacgaat gtcaaaacgg ttctcaatct ccacaatcaa ctcaaccgtt
    32941 cccatactat ccaagacacc tgcatcaaaa agatcttcat ccatcatgtc agaaacatct
    33001 tccataaaca actcatcaat aatttcaata acttctgatt tgatatccat attttatttc
    33061 cttttatttt ttaaaccata gattattcaa gaatccagaa aagattaaga atgacaacat
    33121 gacaacatgg aaagtgacaa ccatgccaag caactgaatc cagcgattct caggtagggc
    33181 agccttccct gcttttttcc gttccttatt gagcgttttt ttcttgcgaa cccaggcatc
    33241 attgatgacc aagcctagtc catgaaagag tccataggcg atatagtacc aggtcacacc
    33301 atgccaaaat cccataatca gcatatttac aatgtaagcc atgcttgagg ttacattacg
    33361 atttttaaag actttctttc tggttaacac catcaccatt cgcataaaga caaagtcacg
    33421 gaaccagaag gacagactca tatgccagcg attccaaaac tcctttaaat cccttgataa
    33481 aaagggcttg ttaaagttga tagggctacg gattcccatc aagtttgaga tggccaaagc
    33541 aaacatagaa taacctgcaa agtcaaagaa gagctccaga ccaaaagtat acataactgc
    33601 caaggcatag agattaaaga agccacctga ctgcaaggct aaattcttca gaggaggtag
    33661 taaggtctct cctaaaacat gagctaggat aaacttatac aaaaagcccc acatgatata
    33721 gcggacagat tcatccagca tatccatcaa ctcatctcgc tcaggaatag cctgataatt
    33781 ttcattaaat cgcttaaagc gatcgattgg accactcgag aaagttggca tgaagagaag
    33841 gaaacggagg aattcccaga gggtaaaatc cttaatcact ccatctctca gctcgatgac
    33901 aattccaacc gaacgaaagg tcaggtaaga aattcccaag aacccaagca aagactgcgt
    33961 tccattgata gctggttgca ccttgacaaa gataatcgga agtagggaca gaaaactaac
    34021 taagtagaag acccacttgc catccttgct ttttcgataa tgcttgtaga aaagcaggag
    34081 caatatttcc cagcaaaggt aaatacccaa ggcagctagt tgattggtct ttccacccac
    34141 caacatggtg acaataaaga agagacttac caacacttca taccaggcaa agcgtttctt
    34201 gaaaaagaga cctataaaga tgggcaaggt tgcagcaatc acataaacaa aatactgagg
    34261 attgccatat ggctctaaat gaggaagctg ttgaaaaaac tccatcatct cttattcacc
    34321 tcgttaatca atcctttgat gtcaatcttt ccatttggag ttagtggcaa actgtctcgg
    34381 taaaggaatt tagatggcat catataggac atcatgatgt ctgtcaggtc ttccttgatg
    34441 gccttggtaa tatcgatatc tcgctcaaac tgctcacgaa caccgtcttt taagatgaca
    34501 taagccaata gattttgtac cttgtggtcc ttgttatagc gcggtactgc gacagcagat
    34561 tcgataaagc gagacttgtt gaggttttga gagacatctt ctaactcaat gcggtaaccg
    34621 ttaaacttaa tctggaagtc catgcgtccg ccgtagagaa gcaagccctc atctgtcatg
    34681 gttcccacat cgcctgtgtg ataggctggc agatcttcaa actcaaagaa ggcttctgct
    34741 gttttttcag gattgttcat ataacctttt gaaacagctg gcccagaaac aatgatttct
    34801 ccctgctcac catttggcag tttatttcct tcctcgtcaa tgataaaggt tggagaatca
    34861 gccttggtat agccgattgg taggcgtttg agagtcgcta acatctcgtc tgtcacggca
    34921 actgctgaca gagctactgt cgcttctgtt gggccgtaag cattgatgat acgggcattt
    34981 gggaaacgct cgcgcagttt ttgagctgtt ttgaccgtca attcttcacc atcaaagtag
    35041 aaatgcgtga ttccaggcat tttctcactg ttgaagtatt cagacaacat ggccatatct
    35101 gcaaaggatg gtgttgatgt ccagatagcg attggcaatg aaaagatagc cgcaaagagt
    35161 tgcttaaagt cctgagtgat gactgaagga agagtgaaaa gcgtaccacc aagtgccaag
    35221 gtcggtgccc aatacatgac agacaagtca aaagaataag gtggctgtgc cagcatttgc
    35281 ggacgactcg gtgtcgcaaa ttccttatcc gtaatcatcc agtttgtaaa gctgaggaga
    35341 ttatcatgtg aaatctgcac tcccttaggc ttaccagtcg taccagaagt aaagataatg
    35401 tagtaattat catctccctt gactggatgc gtgatttcat agttattccc ttgggcaaag
    35461 gcttcttgaa cctgagctag atttatcatt ggtgtagaaa cctgctccaa gggaaaggct
    35521 gaaatggcaa taatcaagct tggctctgct acttctaaaa tagctgaaac tcgctccaag
    35581 gccgaatggc tatcaattgg aatgtaggca tgacctgact tagtcagcgc tacaaaggtt
    35641 gccaacattt catattcttg gccaccaaaa acaaccacag gagacttctc aggcaagcct
    35701 agttggtcaa tgactgcagc caaactatcc gaatcagcct ttaaatcgcc ataagtgtgt
    35761 tcctgcccca aaacattata gacaggatag ctaggctgtg tctgagcaaa atgctcaatg
    35821 gtttcaatca tatctgctat tggtttattt gacacaatag ggattctcct tcaagttaaa
    35881 attcattata gataaagctt ccttgaccct gaccaagata gctaaagaag taaagcagcc
    35941 ctagaaagat aagaaaatac aaggctgtcc gaccaagaaa gaggtacaat tcttttctct
    36001 gtttcatcaa gaaaaaccat tcatttctgt aatttttcgc taaaataaga gtgattctta
    36061 ctagcttatt tttctaccat tgtaccactt tatatagtat cttttcaatt gtttaccgta
    36121 tgtttccaat agatttcagc ttattttaag gattatacag tttttctatg tatattttca
    36181 aatagagtga tcctgcttca aaactccatt tcaggagaca atgaagtaaa tcttcccata
    36241 ataaaacaca caatatcaag ttttttcaac acctgatact atgcgctttt ctgattttta
    36301 aagacttttt aaccactctc tcatttaaaa taatctcgtc tgatataaat taaaatagct
    36361 tctatcatca gacaaatggc tgatagccaa aaactgatgc taataccaaa actctcagta
    36421 atatagctca ttagcaaaac aaatactgaa aatgctaatg tagaaatcac ttcaagaacg
    36481 gaatagacat taactaaatg attttcctct actgtttcct gaagaaatac actttcagga
    36541 acttctttta gttgcaataa cataccaact aaagctgaaa ataataaaaa catctgtgcg
    36601 tttggaaaat atagaatagt cagtgtcact attgccatag ctacaagagg aaaaagaata
    36661 ctttcccatt tagcagcaag gaacttttca gatagcctaa aagcaattaa gccactaatt
    36721 ataataccaa tagagtatgc tgtattagaa tatccccagt aactttccgt tttatttaat
    36781 aactccgtta caaaaacaag tataatggaa gaaacccaaa tcgtatttga aaaaatttcc
    36841 aataaatttg ctgatacaaa aagtcttaat ctaggatttc tagcaactaa cttccaacct
    36901 ttgagcaaaa tttcaagatt agtttctgac tctaacacct ccacttcagc gttaggaaga
    36961 aataacatca gaaagctaga aatgatatac aagactaaat tgatacacgt ggtaggtaac
    37021 agaccaattg ttgcaaacaa gagtccacct aatccccaac ctatcaattg aacagcttca
    37081 ccagtcattg ataaggctga attagcctta cccaaatcgg tcgcatagcg tggcacaata
    37141 gcataggaaa cgggtgctgc aaaaccatct agtatggaaa ttgcaacaac aaatagatag
    37201 gtcaccaaag gcgctacgga ttgcattacg gtaaacattc ctaccagtat cgccaataat
    37261 atagtctttc caaattgaga taaagataaa accctattta gcgctaacct tttagtaacc
    37321 aacggaacta aaagactcgc aacaaaagag gatattccta ttaagatagg aactagtgat
    37381 gtggcaatta ctgattttga aataatgtat atgttagcaa tgattgttac tctaaagaaa
    37441 atatctgcta aatttgcaaa taattgagag actaacaact tgacaaatga attagacatg
    37501 tagcgctccc cctttagtta caaactttgg aattaaattt gatttttaat attttagcat
    37561 aaaatagcag aaaacaaaag tgaggttttt tacatgaaaa aacaaaaact attgccttta
    37621 ttattcctaa ttttaggagg aattatgatt atcgttgttg gctagtacta agaagttagt
    37681 tagttagtag aataattaca tcttcaatcg aaaaagagac tggaaaaagt cttaaaaatc
    37741 agaaaaacgc tcaaatcctt tggcgctcta agcttttcta atgatagatt tagatagtca
    37801 aaaaaatatc tgaaaagttc ccaggatcgt cgtctcagca tcaaaaatac gtgaaatttg
    37861 cataattaaa ataaatttca ggggagttaa tcgagtaggg gataatctct agcccctctc
    37921 acaccaccgt acgtgccgtt cggcatacag cggttcaact aacttttaac gcatgtcgtt
    37981 caaggtaata atccaaacac gaaaccagtc cacgtttttc caggactggt tttgatatag
    38041 cacgtttaag taccgacttc tgagctacta attgataatg gtcgccccag ccagatacct
    38101 tatctgctat ccatttagga actcctaact taagcaatcc ccataatcgt ctcgatttct
    38161 tcttccattg cttccagata atcactcgta ggcgagtacg caagcgctca tctatgctgg
    38221 cgactatact ttcatatttc ccaatgagaa atagtttatc catcctcgaa tagacaaatt
    38281 cagttgctca atacgtcttg ttaggtctat actccatttc ctatgtgtta gtttcttcaa
    38341 tttaagctta aatctccgaa cactatcttg atgtggacgg cttttccaac catctgatga
    38401 tttccagaac ccaaaaccta gatatttcaa ctctcttggt ctggtaatct tagtcttggt
    38461 catgtttact ttcaaaccta gccgtttctc aataaaacga ctgactgaat acatcacacg
    38521 cttagaggct gcctcgcttc cgaccgtaat cacacaatca tctgcgtagc gcacaaatcg
    38581 aagtcccctc ttttctaatt ccttgtccaa ttcattaagc atgatattgg ataagagagg
    38641 agataaattt cctccctgtg gtgtaccaac tagtgtttta taacgttgac cattaatgat
    38701 aacacctgaa tgaagatact tacgaatcaa ggattccgta tctccgtctt cgataatgtt
    38761 atgtactaag gacatcaatc tatcttgagg aactgtatcg aaaaatttct ctaggtctaa
    38821 tccactatcc actcatagcc gtcatttaag tattctaaga gcttcatgat ggctttttca
    38881 catgacctat ttggtctgaa accataactc gtatctgaga aatggggttc acaaatgggg
    38941 ctcatgactt ggacaatggc ctgttgaatc attctatcca taactgttgg aattcctagt
    39001 tgacggatgc ctccgtctgg tttaggtatc tcaactttaa gaactggttg aggcttatat
    39061 tttctctgtt ttatcagttc cttagtcaag cgccagtttt gtctgagata attatccatc
    39121 tcttcgatag tcattccatc aatcccagct gagcctttat tggattttac ttgattgtag
    39181 gcttccagca tattttcgcg tgataatatc ttatctagca gttttgacat gtgcgtattc
    39241 ctttcttgtt ttggtgcctg agggacatct tatattggtg agccttcatc tagtcaatac
    39301 gtgacagact ccagttctat cagacctctc gttttacaga cacaagtgaa gtaggattac
    39361 ttcaatctgt cgttttctct ttctagcgac agacctcaaa cagtctctcc ccagactgtt
    39421 tgagtattca ccccttcgct ccacttccat tacggaagtt tcctcactac tatgggttcg
    39481 gctgacttct catgattcgt tgttactacg ctttcagcac tcatgagacc tcacgggata
    39541 agtcgtacat ctttcctcgt ttatacaaga tatgaggacg ttaaaaggtt acagtgaaaa
    39601 taggaaatct gacgcagagt ctttagactc tagaaagatt tatcttttca cacaaacctt
    39661 agtccgaatt caattactct cgtgatttac acatataggt tacgactacc tttttggact
    39721 ttagagtttg aagcctcctt atccgctata tacgccttac tatcacgttc
//
LOCUS       DAVHIT010000007        39208 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVHIT010000007 DAVHIT010000000
VERSION     DAVHIT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.007"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..71
                     /locus_tag="VVN16_000287"
     CDS             <1..71
                     /locus_tag="VVN16_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU5571739.1"
                     /translation="IPAMSAPVFDNIEAAIKEHGLS"
     gene            119..1594
                     /locus_tag="VVN16_000288"
     CDS             119..1594
                     /locus_tag="VVN16_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU5571740.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2034..8735
                     /locus_tag="VVN16_000289"
     CDS             2034..8735
                     /locus_tag="VVN16_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5571741.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(8983..9840)
                     /locus_tag="VVN16_000290"
                     /pseudo
     CDS             complement(8983..9840)
                     /locus_tag="VVN16_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10051..10410)
                     /locus_tag="VVN16_000291"
     CDS             complement(10051..10410)
                     /locus_tag="VVN16_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU5571742.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMVVELFLMK"
     gene            10502..11459
                     /locus_tag="VVN16_000292"
                     /pseudo
     CDS             10502..11459
                     /locus_tag="VVN16_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; frameshifted;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11443..12000
                     /locus_tag="VVN16_000293"
     CDS             11443..12000
                     /locus_tag="VVN16_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5571743.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            11993..12247
                     /locus_tag="VVN16_000294"
     CDS             11993..12247
                     /locus_tag="VVN16_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571744.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12259..14313
                     /locus_tag="VVN16_000295"
     CDS             12259..14313
                     /locus_tag="VVN16_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5571745.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHSFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14492..15352
                     /locus_tag="VVN16_000296"
     CDS             14492..15352
                     /locus_tag="VVN16_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5571746.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15563..16273
                     /gene="ccdA2"
                     /locus_tag="VVN16_000297"
     CDS             15563..16273
                     /gene="ccdA2"
                     /locus_tag="VVN16_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU5571747.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16293..16859
                     /locus_tag="VVN16_000298"
     CDS             16293..16859
                     /locus_tag="VVN16_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU5571748.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16870..17982
                     /gene="msrB"
                     /locus_tag="VVN16_000299"
     CDS             16870..17982
                     /gene="msrB"
                     /locus_tag="VVN16_000299"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5571749.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKTVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18205..18942
                     /locus_tag="VVN16_000300"
     CDS             18205..18942
                     /locus_tag="VVN16_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5571750.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEGR"
     gene            18939..20630
                     /locus_tag="VVN16_000301"
     CDS             18939..20630
                     /locus_tag="VVN16_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5571751.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            20746..21309
                     /locus_tag="VVN16_000302"
     CDS             20746..21309
                     /locus_tag="VVN16_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU5571752.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21331..27039
                     /locus_tag="VVN16_000303"
     CDS             21331..27039
                     /locus_tag="VVN16_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5571753.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVEAEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKGPEQPVQPTQAEQPRIPKDSSQPEDPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTTEV
                     VPDDTKYVDEEVVERQGSKGVQVTKTTYETVEVVETDKVLSTTTEVKTPAVPKVVKKG
                     TKPVKGTAVETREEVIPFATKEQEDDTLKRGTRQVAQEGVNGKKQITETYKTIRGEKT
                     NEAPTVEETVLQAPQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVE
                     IKSIKVALKDNTGTVIKEVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETETL
                     EDKEVQLDLKKVEIKNIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKT
                     TRLAVSSVEEVVVDGKTLYKVVAKAPDLVQRREDDTLSEEYVHYFEKQLPKVDNVYYS
                     FNELITDMQKNPTGTFKLGADLNAANTPTPSKSYVTGEFKGKLSSVDGQHYTIHNTAR
                     PLFNNIVGGTVKNINLNNVNIDMPWADQIASVANVIKGGSLIENVKVKGNVLGRNWVS
                     GFIDKIDSGGRLVNVAFIGNVTSVGDGDKFLTGIVGENWKGYVEKAYVEANIKGKRAK
                     AAGISYWSQNSGDNNAVGRDGAIKKSVVKGSIDVEKPIEVGGAVGSLNYLGYIEDTVA
                     MMKVKNGEIFYGSHDIDTDPYYTGERVNRNFIVDGVSEGKSSYTYSKQQKRIKSISQE
                     EADKKIKELGITADKFTIIDPIVNKLNNIKQKADTYKDTQDYKADRELTYRNIEKLQP
                     FYNKEWIVDQGNKVPSNSKLLTTEVLSVTGIKSGQFVTDLSDIDKIMIHYSDGAKEEL
                     NVTRQESNVQQVREYSITDLDILYTPNMVEKDRAQLMTDVKSKLSSVELESDGVRQLL
                     VKRDTKKDANANSVGRQNGYIRDLFLEESFSEVKANLDKLVKQILENEDHQLNDNELA
                     ERALLKKVEDNKAKIMMGLAYLNQYYAFKYDELSIKDIMMFKPDFYGKTASVIDRLIN
                     IGSAENNLKGDRTQDAYRGIISGATGKGSLHDFLTYNMKLFTNETDINVWFKKAIEKN
                     AYVVEQPSTNPAFANKKYRLYEGINNGQHGRMILPLLNLKNAHLFMISTYNTISFSSF
                     EKYGKDTDEKREKFKSEINKRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDN
                     HNSPNGWASRHGHIDGKPDYAPIREFFGRINKYHGYKYGYGAYAYIFAAPQPMDAVYF
                     VMTDLISDFGTSAFTHETTHVNDRMAYYGGHWHREGTDLEAFAQGMLQTPSVSNPNGE
                     YGALGLNMAYERQNDGNQWYNTNPNDLTTRAEIDNYMKGFNDTLMLLDYLEGEAVLNK
                     ANQDLNNAWFKKVDKQLRGASTKNQYDKVRDLTADEKNITLNSVDDLVDNNFMTKHGP
                     GNNVYDPTGFGTAYVTVPITAGIYGGNTSEGAPGSMSFKHNTFRMWGYYGYEKGFLNY
                     ASNMLKNESRQAGHNTLGDDFIIKKVSDNKFSTLEDWKKAYFKEVVDKAKAGFNPVTI
                     DGTTYSSYDDLKNAFAAAVEKDKATLKNGSVKSDNTVALKEKIYKKLLQQTDSFKTSI
                     FR"
     gene            27100..28821
                     /gene="pabB"
                     /locus_tag="VVN16_000304"
     CDS             27100..28821
                     /gene="pabB"
                     /locus_tag="VVN16_000304"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU5571754.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLTVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            28822..29439
                     /locus_tag="VVN16_000305"
     CDS             28822..29439
                     /locus_tag="VVN16_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5571755.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29489..30487
                     /locus_tag="VVN16_000306"
     CDS             29489..30487
                     /locus_tag="VVN16_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU5571756.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYARNAWQGN
                     YYLKSDGKMAKGEWVYDATYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKTYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            30614..31573
                     /locus_tag="VVN16_000307"
     CDS             30614..31573
                     /locus_tag="VVN16_000307"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU5571757.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            31670..32509
                     /locus_tag="VVN16_000308"
     CDS             31670..32509
                     /locus_tag="VVN16_000308"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU5571758.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32556..32726)
                     /locus_tag="VVN16_000309"
     CDS             complement(32556..32726)
                     /locus_tag="VVN16_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU5571759.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            32847..33731
                     /gene="miaA"
                     /locus_tag="VVN16_000310"
     CDS             32847..33731
                     /gene="miaA"
                     /locus_tag="VVN16_000310"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU5571760.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33724..34962
                     /gene="hflX"
                     /locus_tag="VVN16_000311"
     CDS             33724..34962
                     /gene="hflX"
                     /locus_tag="VVN16_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU5571761.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            34955..35578
                     /locus_tag="VVN16_000312"
     CDS             34955..35578
                     /locus_tag="VVN16_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU5571762.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            35593..36522
                     /locus_tag="VVN16_000313"
     CDS             35593..36522
                     /locus_tag="VVN16_000313"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU5571763.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36543..37298
                     /locus_tag="VVN16_000314"
     CDS             36543..37298
                     /locus_tag="VVN16_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5571764.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37352..38260)
                     /locus_tag="VVN16_000315"
     CDS             complement(37352..38260)
                     /locus_tag="VVN16_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5571765.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38324..38593
                     /locus_tag="VVN16_000316"
     CDS             38324..38593
                     /locus_tag="VVN16_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU5571766.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38593..38973
                     /locus_tag="VVN16_000317"
     CDS             38593..38973
                     /locus_tag="VVN16_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5571767.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39062..>39208
                     /locus_tag="VVN16_000318"
     CDS             39062..>39208
                     /locus_tag="VVN16_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571768.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYK"
BASE COUNT        12081 a         7284 c         8768 g        11075 t
ORIGIN      
        1 gtatcccagc aatgagcgct ccagtatttg acaatattga agcagctatt aaagaacatg
       61 gattaagcta attctaattt gttaacattc atataacata aaaaagggag gaactgttat
      121 ggatgcaatc tttgacctaa tcggaaaggt tttcaatccc atcttagaaa tgggtggacc
      181 tgtcatcatg ttgatcattt tgacagtatt ggctttactt tttggagtga aattctccaa
      241 agcgcttgaa ggtggtatca aacttgccat cgctcttaca ggtatcggtg ctatcatcgg
      301 tatgctaaat ggtgctttct cagcatcact tgcaaaattc gttgaaaaca ctggtatcca
      361 attgagtatt acagacgttg gttgggcacc actcgcaaca atcacttggg gttctgcttg
      421 gacactatac ttcttgctca tcatgttgat tgtcaacgta gtgatgcttg ctatgaagaa
      481 aaccgataca cttgatgtcg atatctttga tatctggcac ttgtctatca caggtctctt
      541 gattaaatgg tatgctgata acaatggtgt gagtcaaggg gtttcactct ttattgctac
      601 agcagctatc gtccttgtcg gtgtgttgaa aattatcaac tctgacttga tgaaacctac
      661 atttgatgac cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta
      721 catgatgaac ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct
      781 tgataaatat gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa
      841 attcttcatc ggtttcatcc ttggtatcgt tatcggtatt atgggaactc cacatccaat
      901 tgcaggtgtt gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg
      961 tttgactgcc ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc
     1021 cgtagaacca ctatcacaag gtattacaaa cgttgctact aaacgtcttc gaggacgtaa
     1081 attcaatatc ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc
     1141 caacgtactt gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg
     1201 tatcttgcca cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac
     1261 tcgtggtaaa ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct
     1321 ttcaggtaca cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc
     1381 agaaggtgtg agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa
     1441 acttcttggt tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc
     1501 agtagtcttc cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa
     1561 acgtaacgaa gagtacgcag caaaagcaaa ataatgcgct cctataaaat ataaactcaa
     1621 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctaaaaacac agttttgagg
     1681 ttgtagataa gactgacgaa gtcagctcaa aacatggttt tgaggttgta gataagactg
     1741 acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa gtcagctcaa
     1801 aacatggttt tgaggttgta gatgaaactg acgaagtcag ctcaaaacat ggttttgagg
     1861 ttgtagatga aactgacgaa gtcagtaatc atatctacgg caaggcgacg ctgacgaagt
     1921 ttgaattgga ttttcgcaga gtgtaaattg ttgaaactag gttgtcatac catgtatacc
     1981 acttgggctt tggtagtagc taactgcact aaatataata taaggagggt tttatgggga
     2041 aaggccattg gaatcggaaa agagtttata gcattcgtaa gtttgctgtg ggagcttgct
     2101 cagtaatgat tgggacttgt gcagttctat taggaggaaa tatagctgga gaatctgtag
     2161 tttatgcgga tgaaacactt attactcata ctgctgagaa acctaaagag gaaaaaatga
     2221 tagtagaaga aaaggctgat aaagctttgg aaactaaaaa tgtagttgaa aggacagaac
     2281 aaagtgaacc tagttcaact gaggctattg catctgagaa gaaagaagat gaagccgtaa
     2341 ctccaaaaga ggaaaaagtg tctgctaaac cggaagaaaa agctccaagg atagaatcac
     2401 aagcttcaag tcaagaaaaa ccgctcaagg aagatgctaa agctgtaaca aatgaagaaa
     2461 tgaatcaaat gattgaagac aggaaagtgg attttaatca aaattggcac tttaaactca
     2521 atgcaaattc taaggaagcc attaaacctg atgcagacgt atctacgtgg aaaaaattag
     2581 atttaccgta tgactggagt atctttaaca atttcgatca tgaatctcct gcacaaaatg
     2641 aaggtggaca actcaacggt ggggaagctt ggtatcgcaa gactttcaaa ctagatgaaa
     2701 aagacctcaa gaaaaatgtt cgccttactt ttgatggcgt ctacatggat tctcaagttt
     2761 atgtcaatgg tcagttagtg gggcattatc caaatggtta taaccagttc tcatatgata
     2821 tcaccaaata ccttcacaaa gatggtcgtg agaatgtgat tgctgtccat gcagtcaaca
     2881 aacagccaag tagccgttgg tattcaggaa gtggtatcta tcgtgatgtg actttacaag
     2941 tgacagataa ggtgcatgtt gagaaaaatg ggacaactat tttaacacca aaacttgaag
     3001 aacaacaaca tggcaaggtt gaaactcatg tgaccagcaa aatcgtcaat acggacgaca
     3061 aagaccatga acttgtagcc gaatatcaaa tcgttgaacg aggtggtcat gctgtaacag
     3121 gcttagttcg tacagcgagt cgtaccttaa aagcacatga atcaacaagc ctagatgcga
     3181 ttttagaagt tgaaagacca aaactctgga ccgttttaaa tgacaaacct gccttgtacg
     3241 aattgattac gcgtgtttac cgtgacggtc aattggttga tgctaagaag gatttgtttg
     3301 gttaccgtta ctatcactgg actccaaatg aaggtttctc tttgaatggt gaacgtatta
     3361 aattccatgg agtatccttg caccacgacc atggggcgct tggagcagaa gaaaactata
     3421 aagcagaata tcgccgtctc aaacaaatga aggagatggg agttaactct atccgtacaa
     3481 cccacaaccc tgctagtgag caaaccttgc aaatcgcagc agaactaggt ttactcgttc
     3541 aggaagaggc ctttgatacg tggtatggtg gcaagaaacc ttatgactat ggacgtttct
     3601 ttgaaaaaga tgccactcac ccagaagctc gaaaaggtga aaaatggtct gattttgacc
     3661 tacgtaccat ggtcgaaaga ggcaaaaaca accctgctat cttcatgtgg tcaattggta
     3721 atgaaatagg tgaagctaat ggtgatgccc actctttagc aactgttaaa cgtttggtca
     3781 aggttatcaa ggatgttgat aagactcgct atgttaccat gggagcagat aaattccgtt
     3841 tcggcaatgg tagcggaggg catgagaaaa ttgctgatga actcgatgct gttggattta
     3901 actattctga agataattac aaagccctta gagctaagca tccaaaatgg ttgatttacg
     3961 gttcagaaac atcatcagca acccgtacac gaggaagtta ctatcgccct gaacgtgaat
     4021 tgaaacatag caatggacct gagcgtaatt atgaacagtc agattatgga aatgatcgtg
     4081 tgggttgggg gaaaacagca accgcttcat ggacttttga ccgtgacaac gctggctatg
     4141 ctggacagtt tatctggaca ggtacggact atattggtga acctacacca tggcacaacc
     4201 aaaatcaaac tcctgttaag agctcttact ttggtatcgt agatacagcc ggcattccaa
     4261 aacatgactt ctatctctac caaagccaat gggtttctgt taagaagaaa ccgatggtac
     4321 accttcttcc tcactggaac tgggaaaaca aagaattagc atccaaagta gctgactcag
     4381 aaggtaagat tccagttcgt gcttattcga atgcttctag tgtagaattg ttcttgaatg
     4441 gaaaatctct tggtcttaag actttcaata aaaaacaaac cagcgatggg cggacttacc
     4501 aagaaggtgc aaatgctaat gaactttatc ttgaatggaa agttgcctat caaccaggta
     4561 ccttggaagc aattgctcgt gatgaatctg gcaaggaaat tgctcgagat aagattacga
     4621 ctgctggtaa gccagcggca gttcgtctta ttaaggaaga ccatgcgatt gcagcagatg
     4681 gaaaagactt gacttacatc tactatgaaa ttgttgacag ccaggggaat gtggttccaa
     4741 ctgctaataa tctggttcgc ttccaattgc atggccaagg tcaactggtc ggtgtagata
     4801 acggagaaca agccagccgt gaacgctata aggcgcaagc agatggttct tggattcgta
     4861 aagcatttaa tggtaaaggt gttgccattg tcaaatcaac tgaacaagca gggaaattca
     4921 cactgacagc ccactctgat ctcttgaaat cgaaccaagt cactgtcttt actggtaaga
     4981 aagaaggaca agagaagact gttttgggga cagaagtgcc aaaagtacag accattattg
     5041 gagaggcacc tgaaatgcct accactgttc cgtttgtata cagtgatggt agccgtgcag
     5101 aacgtcctgt aacctggtct tcagtagatg tgagcaagcc tggtattgta acggtgaaag
     5161 gtatggctga cggacgagaa gtagaagctc gtgtagaagt gattgctctt aaatcagagc
     5221 taccagttgt gaaacgtatt gctccaaata ctgacttgaa ttctgtagac aaatctgttt
     5281 cctatgtttt gactgatgga agtgtacaag agtatgaagt agatagctgg gagattgccg
     5341 aagaagataa agctaagtta gcaattccag gttctcgtat tcaagcgacc ggttatttag
     5401 aaggtcaacc aattcatgca acccttgtgg tagaagaagg caatcctgca gcacctgtag
     5461 tgccaactgt tactgttgga ggtgaagcag taacaggtct tactagtcga caaccaatgc
     5521 aatatcgtac tctatcttat ggtgcccaat tgccagaagt cacagcaagt gctgaaaatg
     5581 ctgatgtgac agttcttcaa gcaagcgcag caaacggcat gcgtgcaagc atatttgttc
     5641 agcctaaaga tggtggccct cttcaaacct atgcaattca attcctagaa gaagcaccaa
     5701 aaattgctca cttgagcttg caagtggaaa aagctgacag tctcaaagaa gaccaaactg
     5761 tcaaattgtc ggttcgagct cactatcaag atggaacgca agctgtatta ccagctgata
     5821 aagtaacctt ctctacaagt ggtgaagggg aagtcgcaat tcgtaaagga atgcttgagt
     5881 tgcataagcc aggagcagtc actctgaacg ctgaatatga gggagctaaa ggccaagttg
     5941 aactcactat ccaagccaat actgagaaga agattgcgca atccatccgt cctgtaaatg
     6001 tagtgacaga tttgcatcag gaaccaagtc ttccagcaac agtaacagtt gagtatgaca
     6061 aaggtttccc taaaactcat aaagtcactt ggcaagctat tccgaaagaa aaactagact
     6121 cctatcaaac atttgaagta ctaggtaaag ttgaaggaat tgaccttgaa gcgcgtgcaa
     6181 aagtctctgt agaaggtatc gtttcagttg aagaagtcag tgtgacaact ccaatcgcag
     6241 aagcaccaca attaccagaa agcgttcgga catatgattc aaatggtcac gtttcatcag
     6301 ctaaggttgc atgggatgcg attcgtccag agcaatacgc taaggaaggt gtctttacag
     6361 ttaatggtcg cttagaaggt acgcaattaa caactaaact tcatgttcgc gtatctgctc
     6421 aaactgagca aggtgcaaac atttctgacc aatggaccgg ttcagaattg ccacttgcct
     6481 ttgcttcaga ttcaaatcca agcgacccag tttcaaatgt taatgacaag ctcatttcct
     6541 acaataacca accagccaat cgttggacaa actggaatcg tactaatcca gaagcttcag
     6601 tcggtgtcct attcggagat tcaggtattt tgagcaaacg ttcagttgat aacttaagcg
     6661 ttggcttcca cgaagaccat ggagttggtg caccgaagtc ttatgtgatt gagtattatg
     6721 ttggtaagac tgtcccaaca gctcctaaaa accctagttt tgttggtaat gaggaccatg
     6781 tctttaatga ttctgccaac tggaaaccag ttactaatct aaaagcccct gctcaactca
     6841 aggctggaga aatgaaccac tttagctttg ataaagttga aacctatgct gttcgtattc
     6901 gcatggttaa agcagataac aagcgtggaa cgtctatcac agaggtacaa atctttgcga
     6961 aacaagttgc ggcagccaaa caaggacaaa caagaatcca agttgacggc aaagacttag
     7021 caaacttcaa ccctgatttg acagactact accttgagtc tgtagatgga aaagttacgg
     7081 cggtcacagc aagtgttagc aacaatggtc tcgctaccgt cgttccaagc gttcgtgaag
     7141 gtgagccagt tcgtgtcatc gcgaaagctg aaaatggcga catcttagga gaataccgtc
     7201 tgcacttcac taaggataag agcttacttt ctcataaacc agttgctgcg gttaaacaag
     7261 ctcgcttgct acaagtaggt caagcacttg aattgccgac taaagttcca gtttacttca
     7321 caggtaaaga cggctacgaa acaaaagacc tgacagttga atgggaagaa gttccagcag
     7381 aaaatctgac aaaagcaggt caatttaccg ttcgaggcca tgtccttggt agtgatcttg
     7441 ttgctgaggt cactgtacga gtgacagaca aactaggcga agctctttca gataacccta
     7501 actatgatga aaacagtaac caggcctttg cttcagcaac caatgatatt gacaaaaact
     7561 ctcatgaccg cgttgactat ctcaatgacg gagatcattc agaaaatcgt cgttggacaa
     7621 actggtcacc aacaccgtct tctaatccag aagtatcagc gggtgtgatc ttccgtgaaa
     7681 atggtaagat tgtagaacgg actgttgcgc aagccaaact tcacttcttt gcagatagtg
     7741 gtacggatgc accaactaaa ctcgttttag aacgctatgt cggtccagag tttgaagtgc
     7801 caacctacta ttcaaactac caagcctacg acgcagacca tccattcaac aatccagaaa
     7861 attgggaagc tgtgccttat cgtgcggata aagacattga agccggtgat gaaattaacg
     7921 ttacctttaa agctgtcaaa gccaaagcca tgagatggcg tatggagcgt aaagcagata
     7981 agagcggtgt tgcgatgatt gagatgacct tccttgcacc gagtgaattg cctcaagaaa
     8041 gcactcaatc gaagattctt gtagatggaa aagagcttgc tgatttcgct gaaaatcgtc
     8101 aagactatca aattacctat aaaggtcaac ggccaaaagt ctcagttgaa gaaaataatc
     8161 aagtagcttc aactgtggta gatagtggag aagatagcct tccagtactt gttcgcctcg
     8221 tttcagaaag tggaaaacaa gttaaggaat accgtatcca gttgactaag gaaaaaccag
     8281 tttctgagaa gacagttgct gctgtacaag aagatcttcc aaaactcgaa tttgttgaaa
     8341 aagatttggc ctacaagaca gttgagaaaa aagattcaac actgtatcta ggtgaaactc
     8401 gtgtagaaca agaaggaaaa actggtaaag aacgtatctt tacagcgatt aatcctgatg
     8461 gaagtaagga agaaaaactc cgtgaagtgg tagaagctcc gacagaccgc atcgtcttgg
     8521 ttggaaccaa accagtagct caagaagcta aaaaaccaca agtgtcagaa aaagcagata
     8581 caaaaccaat tgattcaagt gaagctagtc aaactaataa agcccagtta ccaaatacag
     8641 gtagtgcggc aagccaagca gcagtagcag caggtttagc tcttctaggt ttgagtgcag
     8701 gattagtagt tactaaaggt aaaaaagaag actagaaatt ttgatacctt ctttatcatt
     8761 aaagaccaaa gaaaaactct tgtcctaaat agaagataaa gagtctggga caaaatagtt
     8821 ctcaaccaca aaaaagctag atatttccaa ttgcggaact ctagcttttt aattttgagt
     8881 cgttctctta ttgtatagta gattgaaact agagtagtac acttctactt ctaaaacatt
     8941 gttagaaatc gatttgactg tccttattga tttgtcctgt tcttatttca tttcactatg
     9001 ttttaggagg ttattggcca taagtaccaa tcccatgtca attctcactt gacgcttgcc
     9061 tctcagatta catctcttgt aacccaaaca agcctttaac tgcccaaaga caggttccac
     9121 atcaatcttg cgttgagcga aaatctgtct accttgggga gataaaagcg cctgacattc
     9181 tttagctttc aagttttgat agcgttcgtt catatatagc ccccttttga ggggctgatt
     9241 caggttcgtc ggcatagtaa accttgattt cctgttgaaa gtctgtctgt gttttctgat
     9301 gtttggtatg gtgaaaacga taataccagc catcaggatg tgtgtagcta tcctccttgt
     9361 cattatagtg ccaattcgct aagtttttag ctgactgttt ataccctctc ttttgttcct
     9421 tatcaaacat ggcatattta atcagaaggg gagtgatttc ttcttgaaaa tagacttgta
     9481 tctgttcttg aagtttggcg gagaatttct ctgtcgtttt cttccacaca aaactatact
     9541 tgttggcatt ggattcaatc ttagttccgt caataaacaa acaatctaag gtcactaact
     9601 cttccatttt tcaatttttc gaccagagaa aatcccttgt gaatagacaa atagaagagt
     9661 agatacaagc attttagggt gataagacgg gcgatcaaag gcatgataga aggcgtagaa
     9721 gtgatgttcc tccaaggtat tcaccacttt ttcaatagta aagacgagat ggtcttgtgg
     9781 taagaaggaa ctgatttcta gtggtaaagt tgtttgattt gtgttatagt gaatatgcat
     9841 gggatagcct ttctaaatgg tttattgtgc aattctattt taccaagact atcgcaacca
     9901 tgcaaaaaag caacggcaaa tccgttgctt tttttggaga taagagacta ttgtcccaga
     9961 ctcttttctt tgtataaaat tgtatagtgt tattgtctat actattttgt agagaaagca
    10021 aaaatcccta gaaaatgctt agggattcga ctatttcatt aagaacaatt caacaaccat
    10081 ctctatatac atgataaggg ttaataaaaa tcctgtacgc cagaagaatt tgatgaattt
    10141 aggatagtaa aagctgcgtt ttttcaaaag gaagaaaaag acgagaataa tggctaggat
    10201 cgagagggct agccccagtc tagggaggaa attatgggta aaggttttag ctgtaatcag
    10261 gtaatattca aataccaaga ccggaaaagc caaatccgca aagtttcgtc ctagtttttt
    10321 taatctaaaa agtttggtta ggataatgca gacgactaag gtcagtatca ataataaaat
    10381 agatgctaat ttcattaaaa tcatacccat attgtatcat aaaaaaaggc taatggaaac
    10441 gagaaacaga ggaatttata ggaaagtcag gcttttgaga ttgtgggtga tttttgttat
    10501 aatgaaagtt ataaaatctt ataatacctt gaatgattat tatcgaaaac tctttggaga
    10561 aaagactttt aaagtcccta ttgatgcggg atttgactgt cccaatcgtg atggaactgt
    10621 ggctcatgga ggctgtactt tttgtacggt ttcaggttct ggagatacta ttgtagcccc
    10681 agatgcgcct atccgtgagc aattttataa ggaaattggc tttatgcacc gtaagtggcc
    10741 ggatgttcag aagtatctgg tttattttca aaattttacc aacacccatg aaaaggtgga
    10801 agttattcga gagcgttatg aacaggctat caatgagcca ggtgtggtag gaatcaatat
    10861 tggaacgcgc ccagactgtt taccagacga aaccatcgaa tatttggctg agttatcgga
    10921 gtgcatgcat gtgacggttg aattaggctt gcagaccact tatgaagcaa cctctgacct
    10981 gattaaccgt gtgcattctt atgaattgta ggtggaaacg gtcaagcgtt tgagaaagta
    11041 tccaaaaatt gagattgttt ccccatttga tcaatggttt gcctggtgaa acccatgaga
    11101 tgatggttga aaatgtccgc cgttgtgtca cggataacga tattcaaggg attaaactgc
    11161 atttgctcca tcttatgaca aatactcgta tgcaacgaga ttaccatgag ggacgtttac
    11221 aattgatgag tcaggacgaa tatgtcaggg tcatctgtga ccaaatggaa attattccca
    11281 aacatatcgt catccatcga attacaggag atgcgcctag ggatatgctg attgggctta
    11341 tgtggagcct caataaatgg gaagctctca acagcattga gatggagatg cgacgtcgtg
    11401 gaagtgttca aggatgcaag gctgtaaaac aggagtttga aaatgaaaag accacttgag
    11461 atggcacatg attttttggc tgaggtcgtg acaaaagagg atgtcgtagt ggatgcgact
    11521 atgggaaatg gtcatgacac gcttttttta gccaagctag ccaagcaagt ctatgccttt
    11581 gatattcaga agcaagcctt ggaaaagacc caagagcgtt tgcatcaggc tggcttgaca
    11641 aatgcccagt taatcttgca aggccatgag acactggacc agtttgtgac aaaagctaag
    11701 gcagggattt ttaatctggg ctatttgccg tcagctgata agtctgtcat cacccgaccg
    11761 cagacaacga ttgaggcatt agaaaagcta tgtggcttac ttgtcaaagg tggacgaatt
    11821 gctattatga tttactatgg tcatgaagga ggcgacctcg agagagatgc tgtcttggat
    11881 tttgtaatcc agttgaacca acaagagtac acagctgcca tttaccgaac tttaaatcaa
    11941 gtcaacaacc cgccgttttt agtgatgatt gaaaaattag agagatatag acatggataa
    12001 acaataccta cgtgaaaagc tggatgccat gcgccagaat tttgttgaat caacccacca
    12061 cgaacgagcg gtgggcgtgc tagaccaagc gcatatgagc aaaaaaatgc ttaaaatcaa
    12121 gaaaaaatta gttgctcttg aaatggaacg gtgccagaga aaaattgagc acaaagactg
    12181 ttccaagatt gaccaaaaaa tcaaagagca gaaggagata tttgaatcct gttgtaaaaa
    12241 agattaagga ggacgtgcgt ggaattactg atttacctca tcctattttt actggtcttg
    12301 attgtctcaa gtacaaccaa taagctcctg ccctttttgc ctctcccttt ggtgcagatt
    12361 cttttgggaa ttgtgattgg tctcttttta cccaatactg actttcatct taatacggag
    12421 ttgtttttgg ccctggttat cggacccttg cttttccgag aggctgaaga agcagatgtt
    12481 acggctattt taaaacactg gcgaatcatt gtttatctca tatttccagt gatttttatc
    12541 tcgaccctga gtttgggtgg cttggcccat cttctttggt tcagccttcc cttggcagct
    12601 tgcttggctg ttggggcagc ccttggtcct acggacttgg tggcctttgc ctctctttcg
    12661 gagcgtttta gctttcctaa gcgcgtgtcc aatattctta agggcgaagg actcttgaat
    12721 gatgcttctg gtttggtggc ttttcaggta gctttgacag cttggacaac tggagctttt
    12781 tctctggggc aagctagcag ttcgctcatc ttttcaatcc taggcggttt tttaattggc
    12841 tttttaacag ccatgaccaa tcgtttcctc catagcttct tgctaagtgt gagagcaacg
    12901 gatattgcta gtgagctttt attagaattg agtttgcctc tagtgacctt ctttctggca
    12961 gaagaagtcc atgtttcagg gattattgcc gtcgtagttg ctggaatttt aaaggcaagt
    13021 cgcttcaaga aaatcacgct cctcgaagcc caagtggata cggtgaccga gacggtctgg
    13081 catacagtga cctttatgct caacggttct gtctttgtga ttttagggat ggagttggaa
    13141 atgatagcag aacctatctt gaccaatcca atctataatc ctctactttt attgctatct
    13201 ctcatcgccc ttacctttgt cctctttgtc attcgtttta ttatgatcta tggctattat
    13261 gcctatagaa cccgacgcct caagaaaaag ctaaataagt atatgaagga catgtttctc
    13321 ttgacctttt caggtgttaa gggaacggtg tccattgcta cgattctctt gataccaagt
    13381 aatctagaac aggagtatcc tctcttgctt ttccttgttg caggtgtgac gcttgtcagc
    13441 tttttaacag gtctcttggt cttgcctcat ctttctgatg aagaggaaga aagcaaggat
    13501 tatctcatgc atatcgccat tttgaatgaa gtaacgctag agttggaaaa agagttggaa
    13561 gacactagaa ataaacttcc cctctatgcg gctattgaca attatcatgg acgtattgaa
    13621 aatctcattt taagccaaga aaaccaggat gatcaagaag actgggctgc tttgaaactc
    13681 ttgattctta gtattgaaag tgatggtttg gaacaggcct atgaagaggg gaacattagc
    13741 aatcgtgctt accgagttta ccaacgttat ctgaaaaata tagaacaagg aatcaatcgt
    13801 aaacttgcct caagactgac ctattatttt cttgtttcct tgaggatttt acgttttctc
    13861 cttcatgaag tttttactct cggaaagacc ttccgtagct ggaagaacaa ggagcaaagc
    13921 cgtctccgtg ctcttgatta tgaccaaatt gcagagctct atcttgccaa tacagagatg
    13981 attattgaaa gtttggaaaa cctgaaggga gtctacagac gctctttgat tagttttatg
    14041 caggagtctc gtcttcgaga aacagctatt atcagcagtg gtgcctttgt cgaacgggtt
    14101 atcaatcgtg tcaaacccaa caatatcgat gaaatgctga gaggctatta tctggagcgc
    14161 aagttgattt tcgaatacga agaaaaacga ttgattacga ctaagtatgc caagaaatta
    14221 cgacaaaatg taaataactt agagaactat tccttgaagg aagctgccaa taccctaccg
    14281 tatgatatgg tggaattggt aagaagaaat tagttaatac tcttcgaaaa tctcttcaaa
    14341 ccacgtcagc gtcgccttac cgtatgtatg tgactgactt cgtcagtctt atctacaacc
    14401 tcaaaacagt gttttgagca gcttgcggct agctttctag tttgctcttt gattttcatt
    14461 gagtataaga ttataagtga aggagtatga catgaaaaag tggtggaaag agctgataga
    14521 caaaccttta ttaaaagctt ttttgcatta ttatcaagca tcagatagtg agttgaccag
    14581 tgtcgcagta gcctactatt ggttgatttc gattttcccc ctgcttttgg tggtggtcaa
    14641 tatcctcccc tattttcaga ttcctgtggg agaatttttg ggctttatga aagacgttct
    14701 gcccccaagc ctctatgaag gtgtggaaaa aatagccaga gaagttttga cgcaaccttc
    14761 aacaggttta ttgagttttt ctgttttatc ggcgttatgg agtttttcaa aatctatgaa
    14821 tttcttgcaa aaagctttta acaaggctta tggcgtcgaa aagagtcgtg gacttatttc
    14881 ccatcagatg ctgagtctcc tagtcagttt tggcttgcag ctcctctttg cgtttgcctt
    14941 gtttttgatc ttatttggtc agatgatttt ggctttactt gctcattatt ggacaaaaga
    15001 tggtattatc tatcaggctt tacaagggtt ggcaggtcct ctgatttacg ccctcctctt
    15061 tgctatcttg gtcatgcttt attattttct tcccaatcta tctaacagga aaattagcta
    15121 taccctacca ggcagtgcct ttgttctctt agtgatttta ggtttactaa ctctgttctc
    15181 tagttatctc aattactatg tccatcattt ggtggatgtc cgaattttag gttccgttct
    15241 tcttgttgtt atgatgtttt ggtttatctt gattgctaaa attgttatcc taggcgcggt
    15301 tatcaatgcc agtatgcaga gtttgaaaga tccggtcttt aaaaaagact aactatttat
    15361 ctattataaa agcgcatagt atcaagactt tatagaacct tgatactatg cgcttttgtg
    15421 ctttgacagt tttttgtata gtttttgtcc aagatttcta aatcatctga ttaaattcct
    15481 attttcccct atcattgtcc ctgttttttc gaaaatggaa ggagtataat aaatctatca
    15541 ataaaatgat aggaggaact ttatgggtca tattttcttt tttctaagtg tctttttggc
    15601 agggattcta tccttctttt ctccttgtat cttacctttg ttaccggtct atacaggggt
    15661 gttactagat gataaggatg gtgctcaggc ttctagcggc aaattttcaa tctcagttac
    15721 tagtttatta cgaactctgg cctttatagc aggaatttcc tttatattta ttttgttggg
    15781 ctatggagct ggttttttag gcgatttgct ttatgcttct tggttccaat atcttactgg
    15841 ggcaattatt atccttcttg gtttgcacca aatggagatt ctacacttta aggggcttta
    15901 taaggaaaag aggctacaac tgcaaggaca ggggcaaaat ggtaagggct atagtcaggc
    15961 atttttattg ggcttgacct ttagttttgc ttggacgcct tgcgtggggc cggttctggg
    16021 gtctgttttg gccttggcgg cttcaggtgg ttcaggagct tggcagggag ctggtctcat
    16081 gttggtgtat acgctgggct tggcgctacc attcttgctt ctagctctga cctctagtta
    16141 tgttttgaaa catttccgaa aacttcatcc ctatctcgga atcctcaaaa aagtgggagg
    16201 ttttctcatt attgtgatgg gactcttggt tctgtttgga aatgcttcaa ttttaagtca
    16261 attatttgaa taaaatggaa aggaatatca atatgaaaaa atggcaaaca tgtgttcttg
    16321 gagcgggttc gctcctttgt ttgacggctt gttcaggcaa gtccgtgact agtgaacacc
    16381 aaacgaaaga tgaaatgaag acggagcaga cagctagtaa aacaagcgca gctaaaggga
    16441 aagaggtggc tgattttgaa ttgatgggag tagatggcaa gacctaccgt ttatctgatt
    16501 acaagggcaa gaaagtctat ctcaaattct gggcttcttg gtgttccatc tgtctggcta
    16561 gtcttccaga tacggatgag attgctaaag aagctggtga tgactatgtg gtcttgacag
    16621 tagtgtcacc aggacataag ggagagcaat ctgaagcgga ctttaagaat tggtataagg
    16681 gattggatta taaaaatctc ccagtcctag ttgacccatc aggcaaactt ttggaaactt
    16741 atggtgtccg ttcttaccca acccaagcct ttatagacaa agaaggcaag ctggtcaaaa
    16801 cacatccagg attcatggaa aaagatgcaa ttttgcaaac tttgaaggaa ttagcctagg
    16861 aggtgtctta tgaatgataa gttaaaaatc ttcttgttgc taggagtatt ttttctagcc
    16921 ataaccggtt tctatgttct attgatacga aatgcagggc agacagatgc ctcgcaaatt
    16981 gaaaaggcgg cagttagcca aggaggaaaa acagtgaaaa aaacagaaat tagtaaagac
    17041 gcagacttgc acgaaattta tctagctgga ggttgtttct ggggagtgga ggaatatttc
    17101 tcacgcgttc ccggggtgac ggatgccgtt tcaggctatg caaatggtag aggagaaaca
    17161 accaagtacg aattgattaa ccaaacaggt catgcagaaa ccgtccatgt cacctatgat
    17221 gccaagcaaa tttctctcaa ggaaatcctg cttcactatt tccgcattat caatccaacc
    17281 agcaaaaata aacaaggaaa tgatgtgggg acccagtacc gtactggtgt ttattacaca
    17341 gatgacaagg atttggaagt gattaaccaa gtctttgatg aggtggctaa gaaatacgat
    17401 caacctctag cagttgaaaa ggaaaacttg aagaattttg tggtggctga ggattaccat
    17461 caagactatc tcaagaaaaa tccaaatggc tactgccata tcaatgttaa tcaggcggcc
    17521 tatcctgtta ttgatgccag caaatatcca aaaccaagtg atgaggaatt gaaaaagacc
    17581 ctgtcacctg aggagtatgc agttacccag gaaaatcaaa cagaacgagc tttctcaaac
    17641 cgttactggg ataaatttga atccggtatc tatgtggata tagcaactgg ggaacctctc
    17701 ttttcatcaa aagacaaatt tgagtctggt tgtggctggc ctagttttac ccaacccatc
    17761 agtccagatg ttgtcaccta caaggaagat aagtcctaca atatgacgcg tatggaagtg
    17821 cggagccgag taggagattc tcaccttggg catgttttta cggatggtcc acaggacaag
    17881 ggcggcttac gttactgtat caatagcctc tctatccgct ttattcccaa agaccaaatg
    17941 gaagaaaaag gctacgctta tttactagat tatgttgatt aatactcttc gaaaatctct
    18001 tcaaaccacg tcagcgtcgc cttaccgtat gtagggttac tgacttcgtc agttttatct
    18061 acaacctcaa agctatactt tgagaaacct gtggctatct tcctagttta ctttttgatt
    18121 ttcattgagt ataagaggtc tttcctaagc agttagagga gaattttgct atactgatac
    18181 tagtaagtgg taaaggagca gatcatgacc tacacaatct taatcgtaga agatgaatat
    18241 ctggtaagac aaggtttgac taaactggtc aatgtagcag cctacgatat ggaaatcatc
    18301 ggtcaggctg aaaatggaag gcaggcttgg gaattgatcc aaaagcaggt gccagatatc
    18361 attttaactg atatcaacat gcctcatcta aatggcatcc agttggccag tctggtacga
    18421 gaaacctatc ctcaggttca tttggtcttt ttaacaggtt acgatgattt tgattatgcc
    18481 ttgtctgctg tcaaactagg tgtggacgac tacctgctca aacccttttc tcgtcaggat
    18541 attgaggaaa tgttggggaa aatcaaacaa aaactagaca aggaagagaa agaagagcag
    18601 ttacaagatt tattgactaa taggtttgaa ggaaacatgg cccagaaaat ccagtctcat
    18661 ctggctgata gccaatttag tttaaagtct ttagccagtg acttaggttt tagtccgacc
    18721 tatctaagtt ccttgattaa gaaagagttg ggcttgcctt ttcaggatta tctggtgaga
    18781 gaacgtgtta aacaagccaa gctcttgctt ttaactacag atctgaagat ttatgagatc
    18841 gcagagaagg ttggttttga agatatgaac tattttaccc aacgttttaa gcagattgca
    18901 ggtgtgacac ctcgtcagtt taagaaggga gaaggccgat gaagcgttct tctcttttag
    18961 ttagaatggt tatttccatc tttctggtct ttctcattct cctagctctg gttggaactt
    19021 tctactatca atcaagttct tcagccattg aggccaccat tgagggcaac agccaaacga
    19081 ccatcagcca gactagccac tttattcagt cttatatcaa aaaactagaa accacctcga
    19141 ctggtttgac ccagcagacg gatgttctgg cctatgctga gaatcccagt caagacaagg
    19201 tcgagggaat ccgagatttg tttttgacca tcttgaagtc agataaggac ttgaaaactg
    19261 ttgtgctggt gaccaaatct ggtcaggtca tttctacaga tgacagtgtg cagatgaaaa
    19321 cttcctctga tatgatggct gaggattggt accaaaaggc cattcatcag ggagctatgc
    19381 ctgttttgac tccagctcgt aaatcagata gtcagtgggt catttctgtc actcaagaac
    19441 ttgttgatgc aaagggagcc aatcttggtg tgcttcgttt ggatatttct tatgaaactc
    19501 tggaagccta tctcaatcaa ctccagttgg ggcagcaggg ctttgccttc attatcaatg
    19561 aaaaccatga atttgtctac catcctcaac acacagttta tagttcgtct agcaaaatgg
    19621 aggctatgaa accctacatc gagacaggtc agggttatac tcctggtcac aaatcctacg
    19681 tcagtcaaga gaagattgca ggaactgatt ggacggtact tggcgtgtca tcattggaaa
    19741 agttagacca ggttcggagt cagctcttgt ggaccttgct tggggccagt gtcacatctc
    19801 ttcttgtttg tctctgctta gtgtggttca gtcttaaacg ctggattgct cctttgaagg
    19861 atttgagaga aaccatgttg gaaattgctt ctggtgctca aaatcttcgt gccaaggaag
    19921 ttggtgccta tgaactgaga gaagtaactc gccaatttaa tgccatgttg gatcagattg
    19981 atcagttgat ggtagctatt cgtagccagg aagaaacgac ccgtcagtac caacttcaag
    20041 ccctttcgag ccagattaat ccacatttcc tctataacac tttggacacc atcatctgga
    20101 tggctgaatt tcatgatagt cagcgagtgg tgcaggtgac caagtccttg gcaacctatt
    20161 tccgcttggc gctcaatcaa ggcaaggact tgatttgtct ctctgacgaa atcaatcatg
    20221 tccgccagta tctctttatc cagaaacaac gctatggaga taagctggaa tacgaaatta
    20281 atgaaaatgt tgcctttgat aatttagtct tacccaagct ggtcctacaa ccccttgtag
    20341 aaaatgctct ttaccatggc attaaggaaa aggaaggtca gggccatatt aaactttctg
    20401 tccagaaaca ggattcggga ttggtcatcc gtattgagga tgatggcgtt ggcttccaag
    20461 atgctggtga tagtagtcaa agtcaactca aacgtggggg agttggtctt caaaatgtcg
    20521 atcaacggct caaacttcat tttggagcca attaccagat gaagattgat tctagacccc
    20581 aaaaaggaac gaaagttgaa atatatataa atagaataga aactagctaa ctcccagtca
    20641 attctgggag ttttatgcgt aattgggcaa actttctagg ttgtctatct tgctaaaaac
    20701 aagaaaaaac ggtatgatag aagagtgaca acagaggtaa cagagatgaa cgagacacag
    20761 attcaaagag aaacacgtca ggtagtggaa gatgttttag aaaagaccaa tttgaagcag
    20821 ggggcccttt ttgttttagg tctatcttct agtgaggtgc taggtggtca gattgggaaa
    20881 gaatccagtc aagagattgg tgagctgata gtggagacta tcttgggtat tctaggtagt
    20941 agaggtatcc atttagcagt tcaaggttgt gagcatgtca atcgggctct cgttgttgaa
    21001 cgtcaggtgg cagagcagtt tggtctggaa attgtcagtg tccatcctac tcttcatgca
    21061 ggaggttcgg ggcagttggc agcctttaag tttatgcagg atccagttga ggttgaattt
    21121 atcaaggctc atgctggatt ggatatcgga gacactgcaa ttggcatgca tgtcaagcat
    21181 gttcaggttc cgattcgccc tattttgaga gagattggcc atgcccatgt aacggcactg
    21241 gctagtcgtc caaaattaat cggaggtgcg cgtgcgcact atccgcaaga tgctattagg
    21301 aagtcttgag aatgaataaa ggagataaaa gtgtttaaaa aagaccgttt ttcaattcgt
    21361 aagattaagg gagttgtagg ctctgtattt cttggaagcc ttttgatggc tccttctgta
    21421 gtggacgcag ccacctatca ctatgtaaat aaagagatta tttcacaaga agctaaagat
    21481 ttaattcaga caggaaagcc tgacaggaat gaagttgtat atggtttggt gtatcaaaaa
    21541 gatcagttgc ctcaaacagg gacagaagca tctgttttga cagcttttgg tttgctgact
    21601 gttgggagct tgcttttaat ctacaagaga aagaaaattg ctagcgtctt tctagttgga
    21661 gctatgggat tggtagttct tcctagtgca ggggctgtag acccagttgc gaccctagca
    21721 ctggctagtc gagagggtgt tgttgaaatg gagggctatc gctatgttgg ttatctatca
    21781 ggtgacatcc tcaaaacgct tggcttggac actgttttag aagaaacctc agcaaaacct
    21841 ggagaggtga ctgtagtcga agttgagact cctcaatcaa caacaaatca ggagcaagct
    21901 aggacagaaa accaagtagt agaggcagag gaagctccaa aagaagaagc acctaaaaca
    21961 gaagaaagtc caaaggaaga accaaaatcg gaggtaaaac ctactgacga cacccttcct
    22021 aaagtagaag aggggaaaga agattcagca gaaccagctc cagttgaaga agtaggtgga
    22081 gaagttgagt caaaaccaga ggaaaaagta gcagttaagc cagaaagtca accatcagac
    22141 aaaccagctg aggaatcaaa agttgaacca ccagtagaac aagcaaaagg cccagaacaa
    22201 cccgtgcaac ctacacaagc tgagcaacca aggataccaa aagattcatc acaaccagaa
    22261 gatcctaaag aagatagggg agcggaagag acaccgaaac aagaagatga acagccagca
    22321 gaagccccag aaatcaaggt tgaagaacca gtagaatcaa aagaggagac taaaacagct
    22381 aaggggacgc aagaagaagg caaagaaggt caagcaccag tacaagaagt aaacccagaa
    22441 tataaagtaa caacaggtac agttgagaag tctaccgaaa gtgaattaga tttcacaaca
    22501 gaagtagttc cggatgacac aaaatatgta gatgaagaag ttgttgaacg tcaagggtct
    22561 aaaggagttc aagttacgaa aacaacttac gaaacagtgg aagttgtaga gacagacaaa
    22621 gtcctttcta caactacaga agtgaaaact cctgctgttc ctaaagtcgt taaaaaagga
    22681 acaaaaccag ttaaaggaac agcagtcgaa acaagagaag aagtcattcc ttttgctaca
    22741 aaagaacaag aagatgacac tttaaaacgc ggaacaagac aagtagctca agaaggtgta
    22801 aatggtaaga aacaaattac tgaaacctat aaaacaattc gcggagaaaa aacaaacgaa
    22861 gctccaacag tagaagaaac agttctccaa gcacctcaag atgagattat caaaaaaggc
    22921 acaaaaggtt tagaaaaacc aaccttagaa tgggttaaaa cagataaaga tgtcttgaag
    22981 aagagtgcga cagccagtta tacattgaat aaaccagatg gggtagaaat caaatccatc
    23041 aaagtagctc taaaagataa tactggaaca gttattaaag aagtaacggt agaagaaaat
    23101 aacttaaacg ctactttaga caacttgaaa tattatcaag gttatacact atccacaacc
    23161 atggtctata atcgaggcga aggcgaagaa actgaaacgt tagaagataa agaagttcag
    23221 ttagacctta aaaaagttga aatcaaaaac atcaaagaaa ctagcttgat gagtgtggat
    23281 gatgctggtg ttgaaacaga taagagctta cttactgaaa aaccgacaga cgttgctcca
    23341 ctttacctac gagtgacaac tcatgataac aaaacaacaa gactggcagt aagttcagtt
    23401 gaagaagttg ttgttgatgg aaaaacattg tacaaagtgg tagctaaagc tcctgactta
    23461 gttcaacgta gagaagatga tacactaagc gaagagtatg tacattactt tgaaaaacaa
    23521 ttaccaaaag tagataatgt gtactatagc tttaacgagt taattacaga tatgcaaaaa
    23581 aatcctactg gtacattcaa actaggtgca gatcttaatg cagcgaatac tccaactcca
    23641 agtaaatcat atgtaacagg tgaatttaaa ggtaaattat caagtgttga tggtcaacat
    23701 tatacgattc acaatactgc gcgcccatta tttaacaata ttgttggtgg taccgttaaa
    23761 aatattaatc ttaacaatgt aaatatcgat atgccttggg ctgatcaaat tgcatctgta
    23821 gcaaatgtga tcaaaggtgg tagccttatt gagaatgtta aagtcaaagg taatgtgctc
    23881 ggcagaaact gggtatctgg tttcattgat aaaattgaca gtggtggtag attagttaat
    23941 gttgcattta ttggtaatgt aacttctgtt ggagatgggg ataaattctt aacaggtatt
    24001 gttggagaaa actggaaagg atatgtcgaa aaagcatacg ttgaagctaa tattaaaggt
    24061 aagcgagcta aagctgctgg tatatcttat tggtcgcaaa atagcggaga taataatgct
    24121 gtcggtagag atggagcaat taagaaatct gtcgttaaag gttcaataga tgtagaaaaa
    24181 ccaattgaag ttggtggcgc tgttggaagt ttaaattatc ttggttatat agaagacact
    24241 gttgctatga tgaaagttaa gaacggtgaa attttctacg gttcgcatga tatcgatacc
    24301 gatccatatt acacaggaga acgagtaaat agaaatttca ttgttgacgg tgtaagtgaa
    24361 ggtaaatctt cgtatacata ctctaaacaa caaaagcgta ttaaatcaat atctcaagag
    24421 gaagcagata agaaaattaa agaattgggt atcacagcag ataaatttac aattatcgat
    24481 ccaattgtaa ataaacttaa caacatcaaa caaaaagctg atacttataa agatactcaa
    24541 gattacaaag cagaccgtga acttacttat cgtaatatcg aaaaacttca accattctac
    24601 aataaagagt ggattgtaga ccaaggtaat aaagttccat caaattctaa attattaact
    24661 acagaagtat tatctgttac aggtataaag agcggtcaat tcgtaactga tttgtcagat
    24721 attgataaaa ttatgattca ctattctgat ggagcaaagg aagaattgaa tgttactcgt
    24781 caagaatcaa acgttcaaca agtgcgtgag tacagtatca cagatttaga tatactctat
    24841 acaccaaata tggttgagaa agatcgcgct cagttgatga ctgatgtaaa gtctaagcta
    24901 tctagtgtag aattagagtc tgatggagtg cgtcaacttc tcgttaagag agatactaag
    24961 aaagatgcga atgcaaatag tgtaggtcgt caaaatggat acattagaga tttattcctt
    25021 gaagaaagtt tctctgaagt taaagcaaat cttgacaaat tggtaaaaca gatacttgaa
    25081 aatgaagatc atcaactcaa cgataacgaa ttagctgaac gtgctttact gaagaaagtt
    25141 gaagataata aagctaaaat catgatggga ctggcatact taaatcaata ttatgctttt
    25201 aaatacgatg agttatcaat taaagatatt atgatgttta aacctgattt ttatggtaag
    25261 acagcaagtg ttattgatag attgattaat attggttctg cagaaaataa tctaaaagga
    25321 gatagaacgc aagatgctta tcgtggtata atttcagggg caacaggtaa aggtagttta
    25381 catgacttct tgacctacaa tatgaaatta ttcacaaatg aaacagacat aaatgtttgg
    25441 tttaagaaag ctattgaaaa gaatgcttat gtagttgaac aaccatcaac gaaccctgca
    25501 tttgccaaca aaaaatatcg tctatacgaa ggaattaata atgggcaaca tgggcgcatg
    25561 attttacctc ttcttaactt gaaaaatgca catctattta tgatttcaac atacaacact
    25621 atctcgttta gttcatttga aaaatatggc aaagacacag atgaaaaacg tgaaaagttc
    25681 aagagtgaga tcaataaacg tgctaaagag caggttaatt acttagattt ttggtcacgt
    25741 cttgctactg ataatgtccg tgataaactt cttaagagtc aaaatgtagt tccaactcct
    25801 gtatgggata atcataatag cccgaacgga tgggctagta gacatggtca tatagatggg
    25861 aaaccggatt atgctccaat tcgtgagttc tttgggcgaa tcaataagta tcatggttac
    25921 aaatatggat atggcgcata tgcatatatt tttgcagcac ctcaaccgat ggatgcagtt
    25981 tatttcgtta tgacagattt aattagtgac tttggaacat ctgcatttac acatgaaact
    26041 actcacgtta atgatcgtat ggcttactat ggtggtcatt ggcatcgtga aggtacggat
    26101 cttgaagcat ttgctcaggg gatgttacaa acgccatcgg tatcaaatcc aaacggagaa
    26161 tatggcgcat taggtctaaa tatggcgtat gaacgtcaaa atgatggaaa tcaatggtat
    26221 aacacaaatc caaacgacct tacaactcgt gcagaaattg ataactacat gaaaggtttc
    26281 aacgatacat taatgcttct tgattacctt gaaggagaag ctgtcctcaa taaagcaaat
    26341 caagatttaa acaacgcttg gttcaaaaaa gtggataagc aacttcgtgg agctagtacg
    26401 aagaaccagt atgataaagt tcgtgattta acggcagatg agaaaaatat cactctaaat
    26461 tcagtagatg atttagttga taataatttt atgacgaaac acggacctgg aaacaatgta
    26521 tatgatccaa ctgggttcgg aacggcctac gtgactgtac ctattactgc aggtatttat
    26581 ggaggtaaca caagtgaagg tgctcctggt tctatgtcat tcaaacataa cacattcaga
    26641 atgtgggggt actacggata tgaaaaagga ttcttaaact atgcttctaa catgctgaaa
    26701 aatgagtcta gacaggctgg tcacaatact ctaggtgatg atttcatcat taagaaagtt
    26761 tctgataata aattcagtac attggaagat tggaagaaag catacttcaa agaagttgta
    26821 gataaagcta aagcaggatt taaccctgtt acaatcgacg gtactacata tagtagttac
    26881 gatgacttga agaacgcatt tgctgcagca gttgaaaaag ataaagctac tcttaaaaat
    26941 ggatcagtta aatctgacaa tactgttgca cttaaagaga aaatatacaa aaaacttctt
    27001 caacaaacag atagctttaa aacttcaatc tttagataat cgaaatctct catctgcttt
    27061 gcggatgagg gttttcttat tttatgctat acttaaggta tgcatagaaa aacagtgatt
    27121 gattttagga ctttagggga gagatatacc tttacccagc ctattaaaga gttgaaaacg
    27181 agaaatgtag aagaagtggc agatttgctg gcacaagtgg aaagctacca agagcaaggt
    27241 tattatgtgg tggggtatgt cagctacgag gctgcacctg cttttgagga gaaattaaca
    27301 gttcacaagg ttcctctact gggcgagtac ttgctttact ttactgttca cgatagggta
    27361 gagacctccc ctattcctct gacttatgag gatattgatt tgccctcaaa ttggcaggaa
    27421 gtgacgtctg cagcagacta tgaaaaggcc attgcccaga tacaccatca cttgaggcag
    27481 ggagacacct atcaggtcaa ctacaccgtc caactcaagc aaaagttaag tgccaatcct
    27541 tttgctatct acaatcgtat ggtggtagag caggaggcgg gctacaatgc ctacgtggaa
    27601 catgacgaga tggcagtgat ttccatgagc ccagagctct tttttgagca aaatgatcgc
    27661 gagttgacaa cacgaccaat gaaggggacg actcagcgtg gggtaactga ccaagaagat
    27721 cttgaacagg ccagttggtt ggaacaggat cccaaaaatc gctctgaaaa tatgatgatt
    27781 gtggacctct tgcgcaatga tatgaaccgt atttctgaag ttgggagcga gcacgtggag
    27841 cgtctgtgtc aggtagagca gtattcaact gtttggcaga tgacttcgac catcaagagt
    27901 cagttgcgag aggatgtgga ccttgttgaa atcttccgct cactctttcc ttgtggttcc
    27961 ataacgggtg caccgaaaat tgcgacaatg gagattatca aggacttgga gcctcaaccg
    28021 cgtggagtct actgtggaac gattggtctc ttgcttccaa atggacgacg gatttttaat
    28081 gtggccattc gtaccattca acttcacaaa ggtcaagcca tttatggagt tggcggaggg
    28141 attacttggg atagcacatg ggaatctgaa taccgagagg ttcatcaaaa ggcagcagtt
    28201 ctttatcgta aacaagctcg cttccaactg attacgacag ggaaaatcag tcagaagaac
    28261 ctgctgtttg aagatcaaca tctggaaaga ctgagaaaag ccagtcgtta ttttgccttt
    28321 ccttttgatg cagaagactt gggacacaag attgaggaag agtgtcagga ttgtgaagct
    28381 aatcgagatt accgcttgcg aatcagcctt agcaaatctg gagagataga agtcaatcgt
    28441 caagtattaa cccctctcag tacaagcttt tgtcaggccc aagtctgtct tcaggaagct
    28501 gctttgaatc aatcctttac ctactttaaa accactcacc gaccgcattt gagcctagga
    28561 gaacaagaga agatttacca caataagtca ggagaactgc ttgaaacctc tataggaaat
    28621 ttggttctga aaatcgctgg aaaactctac acaccgccta tccgacttgg aatcttgcca
    28681 ggaatttacc gtcagcattt gctagaaaca ggacaggtag aagagaaagt cttgaccttg
    28741 gcagatttag cccaagcaga agctatttac ggctgtaatg cagtgagagg cttgtatgaa
    28801 ctaagccttg aggagaacta gatgaaattg atatttttgc atgggttagg gcagtcagca
    28861 gagtcttgga aagaagttcg gaatctactg acagattatc cttctgaggc tatcgagtta
    28921 tttccttctg gagttagtaa ctatcaacaa gctaaagagc gagtttatca gcacctagct
    28981 caagagacag aaccttttgt tttgattgga ttgtccttag gtgctgccct gacactagaa
    29041 ctttccagtt acgatttacc aaatcttcga gctttgattc tgtcaggatg cccgcttaaa
    29101 ttagcaggga atatcctatt ttatcttcaa ttgctgattt ttaaactact tcccaaaaga
    29161 gtatttgaaa aacagggagc agacaagact cttatggtcg gagtttctga ggaattgaag
    29221 acacttgatt taacggatat agcagggact tgctcctatc caaccttact aatttgtggc
    29281 agcaaagata aaccgaatct tagttctatg aaagctctcc atagactgtt aacagattcg
    29341 cagtttcaga tcattcctga tggccctcat gtcttgaata aggcaaaacc aaaggagttt
    29401 gtagaaaaga ccagaagttt ccttgaattg ctgaaataag tttgataaaa taatattgaa
    29461 atcgatatca taattatggg agaaagaaat gaataaacgt ctatttttaa aaatgagtct
    29521 ggtgacgttg ccaattttag ccttgttttc acaaccagtt ttggcggaag aaaacatcca
    29581 tttttcgagc tgtaaggaag cttgggcgaa tggctattcg gatattcacg agggagaacc
    29641 tggttattct gccaagttag accgtgatca tgatggtgtg gcttgcgaat tgaaaaatgc
    29701 tcctaagggt gcttttaaag caaaacaagc ggctacaact caaactgata caacttcatc
    29761 cacagcaagt ggttgggtta agcaggacgg ttcttggtac tattttgatg gaaatggaaa
    29821 tctagtgaaa aatgcatggc agggaaacta ttacctgaaa gctgatggta aaatggcaca
    29881 gagtgaatgg atttatgact cttcttatca agcttggtat tacttgaaat cagatggttc
    29941 atatgctcgc aatgcatggc aaggaaacta ctatttgaaa tcagatggta aaatggctaa
    30001 aggtgaatgg gtttatgatg ccacctatca agcttggtat tacttgaaat cagatggttc
    30061 atatgctcgc aatgcatggc aaggaaacta ctatttgaaa tcagatggta aaatggctaa
    30121 aggtgaatgg gtttatgatg ccacctatca agcttggtat tatttgacat cagatggttc
    30181 ttatgcttac agtacatggc aaggaaatta ctatctaaaa tcggatggta aaatggctgt
    30241 caatgaatgg gttgatggtg gacgttatta tgttggcgct gacggagttt ggaaggaagg
    30301 tcaagcaagt acagcttctt ctagtaatga tagcaatagt gaatattctg ctgctttagg
    30361 aaaggcaaag acttataact cactattcca tatgtcaaaa aaacgcatgt atagacaatt
    30421 aacttctgat tttgataaat tttcaaatga tgcagctcaa tatgccattg atcatttaga
    30481 tgattaacag atactggtag gcgaaaaaat tccttataat tcatttacgc aaacctttgc
    30541 ataagtgaat ggattttgat atactagtac tgtaagcatt ttcaattact acacaatgaa
    30601 aaaggagaat atgatgagtc aaaagattat tgggattgac cttggcggaa cttctatcaa
    30661 atttgcaatc ttaacaacag caggagaaat ccaaggaaaa tggtcaatca agaccaacat
    30721 tttggatgag ggaagtcata tcgttgatga tatgattgag tctattcaac atcgtttgga
    30781 cttgcttgga ttggcagcag cggacttcca aggcattgga atgggatcac caggtgtggt
    30841 tgaccgtgac aaagggactg ttatcggtgc ctacaacttg aactggaaaa cccttcaacc
    30901 aatcaaacaa aagattgaaa aagctttggg cattccattt ttcatcgata atgatgccaa
    30961 cgtagcagct cttggtgagc gctggatggg tgctggtgat aaccaaccag atgttgtctt
    31021 tatgacactc ggtactggtg ttggtggtgg tattgtcgca gaaggcaaat tgcttcatgg
    31081 tgttgctggt gcagcaggtg aacttggtca tatcactgtt gattttgacc aaccaattgc
    31141 atgtacttgt ggtaaaaaag gctgtcttga gacagtcgct tctgcaacag gaattgtcaa
    31201 tttgactcgt cgctatgccg atgaatacga aggcgatgca gccttgaaac gcttgattga
    31261 taacggagaa gaagtaactg ctaagactgt ctttgatctc gcaaaagaag gagacgacct
    31321 tgctttgatt gtttaccgta acttctcacg ttacttggga atcgcttgtg ctaacattgg
    31381 ttcaatccta aacccatcaa caatcgtcat cggtggtggt gtgtcagctg cgggagaatt
    31441 ccttctacaa ggtgttcaaa aagtttacga tgaaaatagt ttcccacaag tacgcacatc
    31501 tactaaatta gctcttgcaa ctctaggaaa tgacgctgga gttatcggag cagcatcact
    31561 tgtattgcaa taagataatg agagaggaaa atgctaacct agagttagta atgttcctct
    31621 tttatttttt atgctatact agttaggaca atgaatgagg tgagagtaaa tgacaaaagc
    31681 agatacgatt tttaaagaga atattgaacg cattctaaag gaaggtgttt tctcagagca
    31741 ggctcgtcct aagtacaagg atgggactgt tgctaattcc aagtatgtaa ctggtgcctt
    31801 ttccgagtac gacttatcta aaggagaatt tcctatcaca accttgcgtc ccattgcaat
    31861 caaatccgcc atcaaagaag ttctctggat ttaccaagac cagtcaaata gcttagaagt
    31921 gcttaatgac aagtacaatg ttcactactg gaatgactgg gaagttggag acacgggaac
    31981 cattggtgag cgctatggtg ccgttgttaa gaaacacgac attatcaata agcttctcaa
    32041 acagttggaa accaatcctt ggaaccgccg caatattatt tctctctggg attaccaagc
    32101 tttcgaagaa acagatgggc tgctcccgtg cgcctttcag accatgtttg atgttcggcg
    32161 tgttgatggg gaaatctatc tggatgcgac cttgacccag cgctccaatg atatgctggt
    32221 ggcccaccac atcaacgcta tgcagtatgt ggctttgcag atgatgattg ccaaacattt
    32281 tggctggaag gttgggaagt tcttctactt catcaacaac ctccatatct atgataatca
    32341 atttgaacaa gctcaggaat tgctccgtcg ggagccgtca aactgccaac cacgcttggt
    32401 tttaaatgtt cctgatggga ctaatttctt tgatatcaaa gcagaagatt ttgagttggt
    32461 ggattatgac cctgttaagc cacagttgaa gtttgaccta gctatttaaa agaatagaaa
    32521 aagaagttga gaataatccc aacttctttt gtttcttaac gtgatacgcg gcgacgagct
    32581 gcttttttac ggttttcttc gatgaaagct gctttttgct cttctggttc gattactttc
    32641 tttttaaatg cgtatactgc acctgcaacg gcagcgacag ttcctgcgac acctgttaca
    32701 agacctttag cgaatccttt agccatgagt cttcctcctt tatattctca atcagccagc
    32761 ctcctcaaga ggtcacattt ttctgactga cctttttgtg ttataataat agtaacgaaa
    32821 aaatgggaat ttttcaagga aaaaagatga aaacaaaaat aattgtgatt gttggaccga
    32881 ctgctgttgg aaagacggct ctagctattg aagttgcaaa gcgttttaat ggcgaagtgg
    32941 ttagtggaga tagccaacaa gtctatcgag gacttgatat tgggacggct aaggctagtc
    33001 cagaagagca ggcagctgtt cttcatcatt taatcgatgt tagagagata accgagtctt
    33061 actcggcttt tgattttgtt tcagaagcta agatgactat tgaggatatt cacagccgtg
    33121 gcaagctagc cattatcgcc ggtgggactg gactttatat ccagagcttg ctagaaggtt
    33181 accacctagg tggggagact cctcatgagg agattttagc ttatcgagct agtttggagc
    33241 catattcaga tgaggaatta gcccatttgg tggagcaagc aggccttgag attccccagt
    33301 ttaatcgtcg tcgtgctatg cgtgccttag aaattgccca ttttggtcag gatttggaaa
    33361 atcaagagac cttgtatgaa ccgctgatta tctgcttgga tgatgaacgt agtcaacttt
    33421 atgagcgtat caaccaccga gtggatttga tgtttgaggc tgggcttttg gatgaggcca
    33481 agtggctgtt tgaccattcc cctaatgtac aggctgctaa aggaattggc tataaggaac
    33541 tctttcctta tttccgtgga gagcagacct ttgaggaagc tagtgagagt cttaaacagg
    33601 cgacccgtcg ttttgccaag cgtcagctga cctggttccg taatcgcatg caggtcacct
    33661 tttatcagat tggagaatct ggtgtacaag accgcatttt aagccagata gaggagtttt
    33721 tagatgattg aaacggagaa aaaagaggag cgagtcctgc tgattggtgt ggaattgcag
    33781 ggtatggaca gttttgacct ctccatggaa gaattggcta gtttagcgaa aacggcaggg
    33841 gcagtcgttg tagatagcta cagacaaaaa cgtgaaaaat atgattccaa gaccttcgtc
    33901 ggctctggta agttggaaga gattgcgctt atggtggatg cagaagaaat cactactgtc
    33961 atcgtcaaca atcgtctgac cccaaggcag aatgtcaatc tagaggaagt tctcggtgtt
    34021 aaggtcattg accgtatgca gttgattttg gatatctttg ccatgcgggc tcgaagccat
    34081 gaagggaagc tccaagtcca cctagcccaa ctcaaatacc ttttgcctcg cttggttggt
    34141 caggggatta tgctcagccg tcaggcaggg ggaattggtt cccgtggtcc tggtgaaagc
    34201 cagctggagc tgaaccgtcg tagcgttcgc aatcaaatca cggatatcga gcgccagctc
    34261 aaggtggttg agaaaaatcg ggcgactgtc agagaaaaac gtttggagtc tagcaccttt
    34321 aagattggtt tgattggtta taccaatgct gggaaatcaa ctatcatgaa catcttgacc
    34381 agtaagaccc agtatgaagc agatgagctc tttgcgactc tggatgcgac aaccaagagt
    34441 attcatctgg gaggcaatct ccaagtaact ttgacagata ccgttggctt tattcaagat
    34501 ttgccgacag agttggtgtc cagtttcaag tcaaccttgg aagaaagcaa gcatgtggac
    34561 cttctggttc atgttatcga tgctagcaat ccttaccacg aggagcatga aaaaacggtt
    34621 ctctccatca tgaaagacct ggacatggaa gatattcctc acttgacgct ttataataaa
    34681 gcggatttgg tggaggattt cacgcctacc caaacgccat ataccctcat ttctgccaag
    34741 tctgaggaca gtcgtgaaaa cttgcaagca ttattgctag ataagattaa ggaaattttt
    34801 gaagcattta ccctgcgagt gcctttttca aagtcctaca agattcatga tttagagagt
    34861 gttgcaattc tggaagaacg tgattatcag gaagacggcg aagtgattac aggctacatt
    34921 tccgagaaaa ataaatggag gttagaagaa ttttatgact gatattaaaa ctttggctct
    34981 aaagtatgga ggttatacaa gtctggacaa ggtctatctg gatcagcttc tagctggtaa
    35041 aacagagcag gagcagttaa cacttattac acctccgcca agtgttgtta atgcctactt
    35101 tgcagaactc tatcagaaaa aaagtcctca ggctgcgacg gattattttg cagaactcag
    35161 tcaggaactg aatctttaca atactgagcc aagtttcacc ctagaaaata agccttttat
    35221 tcggcttaat ctgtctggta aatcctttgg tttttgttat gagagtgacg gctttggtcg
    35281 gattttctct gaaaatgagg agaaaatctc ggatgacttg ctctttgaaa ttgcgcaaat
    35341 tttctcccat caattagtct ttgaagagtc tggtaagatt tacatgaagc ctgtcaagga
    35401 cgaggaagtt gtcagcctag aaagtctcac agctttgact gatttggaaa gattggctga
    35461 tggtcgaaaa cgtctcaaag gctacagcca agaggattta ttgcaagaag ctgctgcttt
    35521 ttctggcaaa cgctatttcc gatcggaaaa ccgcacagcc atgttatata ttgattaatt
    35581 agaaagtatc gaatggatat tcaattttta ggaacggggg ctggtcagcc ctctaaagcc
    35641 cgcaacgttt caagtctcgc cctgaaactt ttggacgaga ttaacgaagt ttggctcttt
    35701 gactgtggag aaggtacgca aaatcgcatt ctggaaacca caattcgacc acgtaaggtc
    35761 agcaaaatct ttattaccca tctgcatgga gaccacattt ttggtttgcc aggtttcctt
    35821 tctagccgtg cctttcaggc caatgaagag cagacagatt tggaaatcta cggacctcaa
    35881 ggaatcaagt catttgtctt aaccagcctt cgtgtgtcag gttctcgtct gccctaccgc
    35941 attcatttcc atgagtttga ccaagattct ctgggtaaaa ttcttgaaac cgataaattc
    36001 actgtgtatg cagaggagct ggaccacact attttctgtg ttggctatcg tgtcatgcaa
    36061 aaggatctag aagggacgct ggatgctgaa aaactcaagg ctgctggtgt tccgttcggc
    36121 ccgctttttg gtaaaatcaa aaacggccag gatcttgttt tggaagacgg aactgaaatc
    36181 aaggcagcag actatatctc agcgccacgt ccaggtaaga ttatcactat tttaggagac
    36241 actcgaaaaa cggatgccag tgtgcgtctg gctgtcaatg cagatgtcct agttcatgag
    36301 tccacttatg gcaagggtga tgaaaaaatt gctcgtaacc atggtcactc aactaatatg
    36361 caagctgcac aagtagcggt agaagcaggt gccaaacgcc tcctactcaa ccatatcagt
    36421 gcccgtttcc tctcaaaaga tataagcaaa ctcaagaagg acgctgccac aatttttgaa
    36481 aatgtccatg tggtcaaaga cttggaagaa gtggaaatct agcagtcaca gaaaggataa
    36541 gtatgcctac tattctcatt accggagcta gcggtggtct agctcaagaa atggtcaaac
    36601 tcctgcccaa tgaccaactc atcttgcttg gtagaaataa ggaaaaatta gcccaactct
    36661 acggaaatta ttcccatgca gaattgattg aaattgatat taccgacgat tcagccctag
    36721 aagctctggt aactgatctt tatctccgct atggcaagat tgatgtcttg attaacaacg
    36781 ctggttacgg gatttttgag ggatttgacc agattgctga taaagatatt caccagatgt
    36841 ttgaggtcaa tacctttgcc ctgatgaatc tgtctcgtca ccttgcggct cgtatgaagg
    36901 aaagcagcaa agggcatatc atcaacatcg tcagcatggc aggtctaata gctactggca
    36961 agtctagtct ttactcagcg accaagtttg cggctattgg tttttcaaat gctctgcgac
    37021 tcgaacttat gccctatgga gtctatgtga caacagtcaa tccaggtcca atccgaacag
    37081 gattttttga ccaagctgac ccagatggaa cttatcttaa atcggttgac cgcttcctct
    37141 tagaggcaga tgcagtggct aaaaagattg tcaagattat aggaaaaaat aaacgagaac
    37201 tcaatctccc gattttgttg aacctagccc ataagtttta tactctcttt cccaagctag
    37261 ctgataagtt ggcaggggaa acttttaatt ataagtaaaa agaaccaatg tgcaggttgt
    37321 tgctagccta catattggtt cttatcattt ctcaactatc aaactgaact tcttctagta
    37381 gatagtcgat aaagcgttcg cccatcttag ataggctggt tttctcatgc tggatataga
    37441 ccagctcaat cgggtcatca atatccagtg ggatagaaac gatattgtct ccgtttaggt
    37501 tgctgttcaa aatccctgtc gcaatggtat aaccatccaa accaatcaag agattaaaga
    37561 gggtggcacg gtcactgacc acaatggatt tcttgtggtg ttcttgagaa agaatctctt
    37621 ctgaaaagta gaaggagttg tgcgtccctt ggtcatagct gaggtaaggg aaattctcca
    37681 aatcagacag tttcaccttg tctttctttg ccagagggtt ggtcttgctg acaaagatat
    37741 gcggttgcgc tgtgaagaga tggtgggcta gtaggtgatt gtcatccagc atcttggtta
    37801 aaacatcacg gttgtaactg tttaagaaga ggaccccgac ctcactgcgg aagttcttga
    37861 cgtcgtcgat aatctcccaa gtccgagttt cacgaaggaa aagctcgtat ttctccatat
    37921 cgcttttctt gagcaaagag acaaaggcat tgaccacaaa ggcatagtgt tgagacgaaa
    37981 cgctaaagag ttcgcggtgg gcgacaggat ttttatagcg ttcctccaga agctgagttt
    38041 gctcgacaac ctgacgggca taagagagaa actccatccc atcacgggtc aaggtgattc
    38101 ccttgggatt gcggataaag atctcaatgc ccatttcatt ttccaaatct ctcacagcat
    38161 tggaaagact tggctgagtg ataaagagtt gcttggctgc ctcattcata gagccagttt
    38221 cgacgatttt gataatataa tgtaattgtt gaattctcat gtttttattg taccacactt
    38281 gccttagaat cgccaaagaa gataggaaaa taaggagcat tgtgtgagtc tcttcttgaa
    38341 aaaaacacaa aattttgcta gaatgagtct tatgaaaaca ttctatgatg tgcagcaatt
    38401 cctcaaacga tttggtatta ttgtttacat gggaaaacgc ttatatgata ttgaactgat
    38461 gaagttggaa ctatctcgga tttacgatgc agggttgatg gataaattag actatctaga
    38521 ggcagaagca gttcttcgca gagagcacaa ggtagaattg gattatattg agaaaaatgg
    38581 agaaaagaac taatggttac ttggattttg tgggcactta tactagcaat gttggcgtgg
    38641 atgggcttta actatcttcg tattcgtcgt gcggctaaaa ttgtggacaa tgaggagttt
    38701 gaagccttga ttcgtacggg tcaattgatt gatttgcgcg acccagcaga attccacaga
    38761 aaacatatcc ttggtgcacg caatattcct tcaagtcagt tgaaaactag tcttgcagcc
    38821 cttcgtaaag ataaacctgt ccttctctac gagaaccaac gtgcgcaacg agttacaaat
    38881 gcagctcttt acttgaaaaa acaaggtttt tctgagattt atatcctttc ttatggcttg
    38941 gattcttgga aagggaaagt gaagactagc taaccttatt tccaaattag tcgaatgata
    39001 ggatgaagct attgttttat agtataatta atttgtataa aatttaaaga gttcttagaa
    39061 catgaaagaa aagaagaaaa atccactttt tgtgggtatc ttgtcgatta ttcttggttt
    39121 gttatttcca attgtaggct tgattttggg tatcatagga ttggtcttgg ctatttcata
    39181 ccaaaaagag tcacaattag actataaa
//
LOCUS       DAVHIT010000008        38320 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVHIT010000008 DAVHIT010000000
VERSION     DAVHIT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.008"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            99..1124
                     /gene="trpS"
                     /locus_tag="VVN16_000319"
     CDS             99..1124
                     /gene="trpS"
                     /locus_tag="VVN16_000319"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5571769.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1276..2754
                     /gene="guaB"
                     /locus_tag="VVN16_000320"
     CDS             1276..2754
                     /gene="guaB"
                     /locus_tag="VVN16_000320"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU5571770.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2805..3902)
                     /gene="recF"
                     /locus_tag="VVN16_000321"
     CDS             complement(2805..3902)
                     /gene="recF"
                     /locus_tag="VVN16_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU5571771.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(3905..4273)
                     /gene="yaaA"
                     /locus_tag="VVN16_000322"
     CDS             complement(3905..4273)
                     /gene="yaaA"
                     /locus_tag="VVN16_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU5571772.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4432..5682
                     /locus_tag="VVN16_000323"
     CDS             4432..5682
                     /locus_tag="VVN16_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5571773.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5679..6962
                     /locus_tag="VVN16_000324"
     CDS             5679..6962
                     /locus_tag="VVN16_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5571774.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTNLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSSKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7004..7825
                     /locus_tag="VVN16_000325"
     CDS             7004..7825
                     /locus_tag="VVN16_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5571775.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEESSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7836..8381
                     /gene="pgsA"
                     /locus_tag="VVN16_000326"
     CDS             7836..8381
                     /gene="pgsA"
                     /locus_tag="VVN16_000326"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5571776.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8378..9205
                     /locus_tag="VVN16_000327"
     CDS             8378..9205
                     /locus_tag="VVN16_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU5571777.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9190..10029
                     /locus_tag="VVN16_000328"
     CDS             9190..10029
                     /locus_tag="VVN16_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU5571778.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10022..10816
                     /locus_tag="VVN16_000329"
     CDS             10022..10816
                     /locus_tag="VVN16_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU5571779.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10875..11693
                     /gene="mreC"
                     /locus_tag="VVN16_000330"
     CDS             10875..11693
                     /gene="mreC"
                     /locus_tag="VVN16_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5571780.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATMHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11693..12187
                     /gene="mreD"
                     /locus_tag="VVN16_000331"
     CDS             11693..12187
                     /gene="mreD"
                     /locus_tag="VVN16_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU5571781.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12281..13459
                     /locus_tag="VVN16_000332"
     CDS             12281..13459
                     /locus_tag="VVN16_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU5571782.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13683..14462
                     /gene="rpsB"
                     /locus_tag="VVN16_000333"
     CDS             13683..14462
                     /gene="rpsB"
                     /locus_tag="VVN16_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5571783.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14541..15581
                     /locus_tag="VVN16_000334"
     CDS             14541..15581
                     /locus_tag="VVN16_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU5571784.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15827..16150
                     /gene="tnpB"
                     /locus_tag="VVN16_000335"
     CDS             15827..16150
                     /gene="tnpB"
                     /locus_tag="VVN16_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU5571785.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGERKDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(16305..16613)
                     /locus_tag="VVN16_000336"
                     /pseudo
     CDS             complement(16305..16613)
                     /locus_tag="VVN16_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16604..17002
                     /locus_tag="VVN16_000337"
     CDS             16604..17002
                     /locus_tag="VVN16_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5571786.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17199..18119)
                     /gene="cysK"
                     /locus_tag="VVN16_000338"
     CDS             complement(17199..18119)
                     /gene="cysK"
                     /locus_tag="VVN16_000338"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU5571787.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18404..19039)
                     /locus_tag="VVN16_000339"
     CDS             complement(18404..19039)
                     /locus_tag="VVN16_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU5571788.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19095..20393
                     /locus_tag="VVN16_000340"
     CDS             19095..20393
                     /locus_tag="VVN16_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5571789.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20390..21052
                     /locus_tag="VVN16_000341"
     CDS             20390..21052
                     /locus_tag="VVN16_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU5571790.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21132..21680
                     /gene="raiA"
                     /locus_tag="VVN16_000342"
     CDS             21132..21680
                     /gene="raiA"
                     /locus_tag="VVN16_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU5571791.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21816..23789
                     /locus_tag="VVN16_000343"
     CDS             21816..23789
                     /locus_tag="VVN16_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU5571792.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGLDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23786..24238
                     /gene="rplI"
                     /locus_tag="VVN16_000344"
     CDS             23786..24238
                     /gene="rplI"
                     /locus_tag="VVN16_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5571793.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24282..25634
                     /gene="dnaB"
                     /locus_tag="VVN16_000345"
     CDS             24282..25634
                     /gene="dnaB"
                     /locus_tag="VVN16_000345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5571794.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25636..25902
                     /locus_tag="VVN16_000346"
     CDS             25636..25902
                     /locus_tag="VVN16_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571795.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGNVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            25991..27337
                     /gene="cbpD"
                     /locus_tag="VVN16_000347"
     CDS             25991..27337
                     /gene="cbpD"
                     /locus_tag="VVN16_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU5571796.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27420..27522
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27593..27883
                     /locus_tag="VVN16_000348"
     CDS             27593..27883
                     /locus_tag="VVN16_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU5571797.1"
                     /translation="MKASIALQVLPLVQGIERIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            27846..28604
                     /locus_tag="VVN16_000349"
     CDS             27846..28604
                     /locus_tag="VVN16_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5571798.1"
                     /translation="MRNLRSILRRHISLLGFIGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            28643..29650
                     /locus_tag="VVN16_000350"
     CDS             28643..29650
                     /locus_tag="VVN16_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5571799.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29650..30378
                     /locus_tag="VVN16_000351"
     CDS             29650..30378
                     /locus_tag="VVN16_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571800.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30497..30955
                     /locus_tag="VVN16_000352"
     CDS             30497..30955
                     /locus_tag="VVN16_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU5571801.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            30957..33389
                     /locus_tag="VVN16_000353"
     CDS             30957..33389
                     /locus_tag="VVN16_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5571802.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            34031..34684
                     /gene="rr06"
                     /locus_tag="VVN16_000354"
     CDS             34031..34684
                     /gene="rr06"
                     /locus_tag="VVN16_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU5571803.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34681..36012
                     /gene="hk06"
                     /locus_tag="VVN16_000355"
     CDS             34681..36012
                     /gene="hk06"
                     /locus_tag="VVN16_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU5571804.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            36095..36622
                     /locus_tag="VVN16_000356"
     CDS             36095..36622
                     /locus_tag="VVN16_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU5571805.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDSMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGRMKG"
     gene            36702..>38320
                     /gene="pspC"
                     /locus_tag="VVN16_000357"
     CDS             36702..>38320
                     /gene="pspC"
                     /locus_tag="VVN16_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEU5571806.1"
                     /translation="MFKSNYERKMCYSIRKFSIGVASVAVASLVMGSVVHATENEGTT
                     QAPTSSNRGNESQAEQRRELDLERDKVKKEVREYKEKKVKELYSKSTKSRHKKTVDIV
                     NKLQNINNEYLNKIIQSTSTYEELQKLMMESQSEVDKAVSEFEKDLSSSSSSGSSTEP
                     EASDTAKPNKPTELEKKVAEAQQKVEEAEKKAKDQKEEDHRNYPTITYKTLELEIAEF
                     DVKVKEAELELLKVKAKESRDEKKIKQAEAEVESKQAEATRLKKIKTDRKKAEEEAKL
                     KEAVEKNAATSEQGKPKRRVKRGALGEQATPDKKDYFEKDFRPAFNKNQQMVAIQESL
                     NKLDDETKTVPDGAKLTGEAGNAYNEVRDYAIKVVSENKKLLSQTAVTMDELAMQLTK
                     LNDAMSKLKEAKAKLVPEVKPQPENPEHQKPTTPAPDTKPIPQPEGKKPSVPDINQEK
                     EKAKLAVVTYMSKILDDIQKHHLQKEKHRQIVALIKELDEFKKQALSEIDNVNTKVEI
                     ENTVHKIFADMDAVVTKFKKGLTQDTPKEPD"
BASE COUNT        12049 a         6240 c         8417 g        11614 t
ORIGIN      
        1 caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt cattaaaaaa
       61 atgctataat tgaaaggact atatcgaagg agaacaaaat gactaaaccc attattttaa
      121 caggagaccg tccaacagga aaattgcata ttggacatta tgttggaagt ctcaaaaatc
      181 gagtattatt acaggaagag gataagtatg atatgtttgt gttcttggct gaccaacaag
      241 ctttgacaga tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga aatgtggctt
      301 tggattatct tgcagttgga ttggatccaa ataagtcaac tatttttatt caaagccaga
      361 ttccagagtt ggctgagttg tctatgtatt atatgaatct agtttcgtta gcacgtttgg
      421 agcgaaatcc aacagtcaag acagagattt ctcagaaagg atttggagaa agcattccga
      481 caggattctt ggtctatcca atcgctcaag cagctgatat cacagctttc aaggctaatt
      541 atgttcctgt tgggacagat cagaaaccaa tgattgagca aactcgtgaa attgttcgtt
      601 cttttaacaa tgcatataac tgtgatgtct tggtagagcc ggaaggtatt tatccagaaa
      661 atgagagagc agggcgtttg cctggtttag atggaaatgc taaaatgtct aaatcactaa
      721 ataatggtat ttatttagct gatgatgcgg atactttgcg taaaaaagta atgagtatgt
      781 atacagatcc agatcatatc cgcgttgagg atccaggtaa gattgaggga aatatggttt
      841 tccattatct agatgttttt ggtcgtccag aagatgctca agaaattgct gatatgaaag
      901 aacgttatca acgaggtggt cttggtgatg tgaagaccaa gcgttatcta cttgaaatat
      961 tagaacgtga actgggtcct attcgtgagc gccgtattga atttgctaag gatatgggag
     1021 aagtttataa tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg ggtcaaaccc
     1081 tatctgaggt aaaaggggca atgggacttc attactttaa ctaagtttat tttacaagaa
     1141 actatcattt ctatctcgaa gaaaagatag ttttttattt acccctgtaa catttgacaa
     1201 atttttatag aatggtatga tagatacaat ataaaaagag tcaagctcaa aaagaaagaa
     1261 aagaggaaac ttcgaatgtc taattgggac actaaatttt tgaaaaaagg ttttaccttt
     1321 gatgatgtat tgcttattcc agctgaaagt catgtgttgc ctaacgatgc agatttaaca
     1381 actaaattgg cagataattt gactttaaat atcccaatta ttaccgctgc catggacaca
     1441 gttacagaga gtcaaatggc cattgctatt gctcgtgcag gtggtctcgg agttatccat
     1501 aaaaacatgt caattgctca acaagcagac gaggttcgta aggtaaaacg ttctgaaaat
     1561 ggagttatta ttgatccgtt cttcttgacg cctgaacata caattgctga agcagatgag
     1621 cttatgggtc gttaccgcat cagtggtgtt ccagttgttg aaacacttga aaatcgtaaa
     1681 ttggttggta ttttgacaaa ccgagatctt cgttttattt cagattataa tcaaccaatt
     1741 tcaaaccata tgactagtga aaatcttgtt actgctcctg tgggtacgga tcttgcaacg
     1801 gctgagagta ttcttcaaga gcatcgtatt gaaaaacttc cgttggtcga tgaagaaggc
     1861 agtctttctg gtttgatcac tatcaaagat attgaaaaag ttattgagtt tccaaatgcg
     1921 gctaaagatg aatttggtcg tcttctagtt gcaggtgcag taggtgttac ttcagataca
     1981 tttgaacgtg cagaggctct ttttgaggca ggagcggatg cgattgttat tgatactgca
     2041 catggtcatt ctgcaggtgt cttgcgtaaa attgccgaga ttcgtgctca tttcccagat
     2101 cggactttga ttgctggaaa tattgctact gctgaaggtg cacgtgccct ttatgaagcg
     2161 ggtgtagacg ttgttaaggt tggtattgga ccaggttcta tctgtactac tcgtgtgatt
     2221 gctggtgttg gtgttccgca agtaacagct atctacgatg ctgcagctgt tgcgcgcgaa
     2281 tatggtaaaa cgattattgc tgacggtggg atcaagtatt ctggagatat tgtaaaagca
     2341 cttgctgcag gtggaaatgc tgttatgctt ggatctatgt ttgctggaac tgatgaagct
     2401 ccaggcgaaa ctgaaatctt ccaaggacgt aaattcaaga cttaccgtgg tatgggatca
     2461 attgctgcta tgaagaaagg ttcaagcgac cgttatttcc aaggttctgt caatgaagca
     2521 aacaagctcg ttccagaagg aattgaaggt cgtgttgctt ataaaggagc ggcagctgat
     2581 attgttttcc aaatgattgg tggtattcgc tctggtatgg gttactgtgg tgcagctaac
     2641 cttaaagaac tacacgataa tgctcaattt attgaaatgt ctggtgctgg tttgaaagaa
     2701 agccatcctc atgatgtgca aattactaat gaggcaccaa attattctat gtaaaaaaca
     2761 atgaaaagaa ctccagtgaa aacaggagtt cttttacaat gttgtcaatt tccatttaca
     2821 gaagctttac catcctgaat agtgaagata cttagatttt ctggcagatt ttgaagatgg
     2881 tctaagcttg ttgttgtgat aaaggtttgg attgattgag aaatcgtttc taataatttt
     2941 aactgtctag tgttgtcaag ttcactcatc acatcgtcaa gcagtaatat aggagattct
     3001 gtggtaatgc tttccattaa ttcgatttct gctaatttta tcgagaggac gagactacga
     3061 tgttgacctt ggcttccgaa actagcatcc atcccattta tataaaaaga aatgtcatct
     3121 cgatgaggac cgacaccagt attcttttta aataaatctc tggatctact tttttctaaa
     3181 gcaattttga aagattcgga taagttttgt ttgtcagtta tattgacaga agattgatag
     3241 gatattgaca actcttcgat ctgattagag agttcaaaat gtttcttacg cccaaatgat
     3301 tctagttttt ttatgaaatc taagcggtga ttcattacac gacatccata atcaactagc
     3361 tgatcatcta acacagaaag gaatgtttca tctatttttt gagctgattt taggtaagtg
     3421 tttctttgct ttaggatgtg gttataattg gttaagtcag ataaatagat tggcttaatt
     3481 tgcccaagtt ccatatcaat gaattttcgt cgaatcgaag gtgctccttt aattagttgt
     3541 aaatcttcag gagcaaataa gacaacattc atgtgtccta cataatctga aaggcgtgcc
     3601 tgttttaagt gattaacttt tgttacacgg cctttttgtg ttagttcgat ttctagggga
     3661 atggatccag tttttttctg aacgagacct gaaagatgaa gttgttcctc atcaaaatga
     3721 atgagatttt tatctgttcg agttcgatga ctacgcgtta aggctaaaaa atagatagcc
     3781 tctaacatgt ttgtttttcc ttgtgcatta cgtcccaaaa agacattcaa tttaggatta
     3841 aagtctattt tcgtttcttt gtaattacga aaagtcttga gagatagatg ttgtagccac
     3901 atgattatct accagggaat cgtggagctt gttggctttt aggtgatgaa gcaggtttcg
     3961 atttgtcttt tttaactccc ttattcatct ttttgacaag tttagcgatc cgttcttttt
     4021 caactttatc agcttggtat tcatcttgtt cttcagaagt aggttgtgtc aacaagatgt
     4081 caatgttcat gtcagggatg tcaactttat cgccaatacg aagttttttt ccacgacgac
     4141 tctctaattc cctattaaag taaacagaat gttcagagag aaatgattta atagctcctc
     4201 cgctatgtgt aattccaagt tctttgagta gtgcttggag ggtaataaat tcttcaaata
     4261 atttgtattc cataattcac ctcaatcttt ggtattatac catattttcc taaaaatagt
     4321 gagagagaca gcgagaaatg taagcgattt ttatttcaaa agtgttacag ggaacaagaa
     4381 aatttcaggt tttcgtgata taatagaagt ctgtatataa ggaggtaaat catggagtta
     4441 gtgcatggaa tttcaacaca ttttatccaa tcaaaaaagt ttaaaacaaa caaaattacc
     4501 gtgcgtttta ccgctccatt atcccttgat acgattgcag gtcacatgtt gagtgcaagt
     4561 atgctagaga ctgctaatca gatgtacccc acttctcaag atttgaggag acacttggcc
     4621 agtctatacg gtacagatat gtcaaccaat tgtttcagaa gagggcaaag ccacattata
     4681 gaattgacat ttacctatgt tcgtgatgag tttttaagta ggaaaaacgt gctaacctct
     4741 cagattttgg aacttgtaaa agaaactctt ttttcacccg tagtagttga taatgggttt
     4801 gatccggcct tatttgaaat tgagaaaaaa caattgctag caagtttagc agctgatatg
     4861 gatgattctt tttattttgc acataaagaa ttggataaat tgttttttca tgatgaacgt
     4921 cttcaattgg aatatagcga tttacgaaat cgtattttag ctgaaactcc acaaagttct
     4981 tattcttgtt tccaagaatt tttagccaat gatcgaatag atttcttttt cctaggtgat
     5041 tttaatgagg ttgaaattca aaatgtatta gaatcatttg gctttaaagg tcgaaaagga
     5101 gatgtgaagg ttcagtattg tcaaccttat tctaatatcc ttcaggaagg tatggttcgg
     5161 aaaaatgtgg gacaatccat tttggaatta ggttatcatt accgttctaa atatggtgat
     5221 gagcaacatt tacccatgat tgtaatgaat ggtttacttg gtggatttgc tcactctaag
     5281 ctctttacaa atgtccgtga aaatgctgga ttagcttata ccatttcaag tgagcttgat
     5341 ttatttagtg gattcttgag gatgtatgct ggtatcaatc gagaaaatcg taaccaggct
     5401 cgtaaaatga tgaataatca actgcttgat ttaaaaaaag gttattttac agagtttgag
     5461 ttaaatcaga ccaaggaaat gattcgttgg tcgttgttac tttctcaaga taatcaatct
     5521 tcattgattg aacgtgctta tcaaaatgcc ttatttggaa aatcttcagc agactttaaa
     5581 agttggattg caaagcttga acaaattgac aaagatgcta tttgtagagt agctaataat
     5641 gtgaaactac aagcgattta ctttatggaa ggaatagaat gacaaaggtt gtttttgaag
     5701 aaaaatacta tccagctgta aaagaaatgg tttatcgaac tcgtttggcc aacggattga
     5761 cagttgctct tttgcctaaa aaggaattta aagaggttta cgggagtgta actgtacagt
     5821 ttggttcgat agatacgctt gtcacagaag ttgacggaga tgtaaaacaa tatcctggag
     5881 gaattgctca ttttcttgaa cataaattat ttgagagaga agattctagt gatttgatgt
     5941 cggcttttac gaatctaggt gcagatagta atgcctttac aagctttaca aaaacaaact
     6001 atcttttttc agcaacggat tattttttag aaaatttata tttacttgat gaattggtaa
     6061 catcagcaca ctttactgaa gcttccattc tgacagagca ggatattatt cagcaagaac
     6121 gagaaatgta ccaagatgat ccagattcgt gtttattctt ttcaacttta gcgaatttgt
     6181 atcctggtac acctttagca actgatatag ttggaagtga ggagtccatt tcccaaatca
     6241 atctaactaa tttgcaagaa aattttacaa agttttacaa acctgtaaac atgtctctgt
     6301 ttttagttgg taattttgat gtggagcgag tacaggacta ttttgaaagc aaagaactga
     6361 aagattcaga ttttcaggaa gtagcaagag aaaagttgtt tttacagcct gtaaagccaa
     6421 cagatagtat gagaatggaa gtatcttctt ccaaactagc gattggagtt agaggtaagc
     6481 gagaagtttc tgaagcggat tgctatcgac atcatatttt attaaaatta ttgtttgcaa
     6541 tgatgtttgg ttggacttcg gatcgttttc aaaaatgtta tgaatcaggt aaaattgatg
     6601 catccttatc tctggaagtt gaagtaacaa gtcgctttca ttttgtcatg ttgacaatgg
     6661 atacgaaaga gccagttgct ttgtctcatc aatttaggaa ggctattcgt aattttacaa
     6721 aggatttaga tattacagag gaacatttag atattatcaa aagagagatg tttggcgaat
     6781 ttttcagtag catgaactct cttgaattta ttgcaacgca atatgatgct tttgaaaatg
     6841 gtgagacaat ttttgatttg ccgaaaattt tacaggaaat tactttagag gatgtccttg
     6901 atgctggaca tcatttaata gatgatggtg acatagttga ttttacaata ttcccatcgt
     6961 agtaacctat tataatagac actagaaaga agggatgaca agtatgagaa aaaaaacaat
     7021 tggagaggtt ttacgattag ctagaatcaa tcagggattg agtttagatg aattgcagaa
     7081 aaagacagaa atccagttag atatgttgga agcaatggaa gcagacgatt tcgatcaact
     7141 tccaagtcct ttttacacgc gttctttctt gaaaaaatat gcatgggctg ttgagttaga
     7201 tgaccaaatt gttttggatg cttatgattc tgggagtatg attacttatg aggaagtaga
     7261 tgttgatgaa gatgagttga caggtcgtag acgttcaagt aagaaaaaga agaaaaaaac
     7321 atcattttta cctttatttt attttatcct ttttgcttta tcgattttaa tttttgtgac
     7381 ttattatgtt tggaactata ttcaaactca accagaggag tcttctcttt ctaattacag
     7441 tgtggttcaa tcaacaagtt caactagctc tgttccccac tcctcaagta gtagttcttc
     7501 tagtatagaa tcagctataa gtgtatcagg cgaaggaaat catgtagaaa tcgcttataa
     7561 gacaagtaag gaaacagtta aattgcaatt ggcagtttca gatgttacaa gttgggtcag
     7621 tgtttcagaa agcgaacttg agggcggtgt aaccttatcg ccaaagaaga aaagtgcaga
     7681 agcaacagtt gcaactaaaa gtcctgtaac aattacgtta ggtgttgtaa aaggtgttga
     7741 tttgacagta gataatcaga ctgttgattt atcgaaatta acagctcaga ctggacaaat
     7801 cactgtaacc tttactaaaa attaaggaaa aacgaatgaa aaaagaacaa attcccaatc
     7861 tcttaacaat aggtcgaatt ctctttatac ctatttttat ctttatttta acgataggaa
     7921 attcgataga gagtcatata gttgcagcta ttatctttgc tgttgccagt attaccgact
     7981 atttagatgg atatttagct cgtaaatgga atgtggtcag taattttggt aaatttgcag
     8041 atcctatggc ggataagtta ctagttatgt cggcttttat tatgttgatt gagttaggta
     8101 tggctccggc ttggattgtt gcagtgatta tctgtcgtga gttagctgtg acaggtttaa
     8161 ggcttttatt ggttgaaact ggtggaacaa ttttagcagc agcaatgcct ggaaaaatta
     8221 aaacttttag tcagatgttt gctattattt tcttgctatt acattggact ttgcttggtc
     8281 aagttctact ttatgtagcc ttatttttca ctatctactc tggctatgac tatttcaagg
     8341 gtagtgccta tgtatttaaa gggacatttg gttcgaaatg aaatcaataa ttgatgtaaa
     8401 aaatctttct tttcgctata aagaaaatca gaactactac gatgtgaagg atattacgtt
     8461 tcacgtgaaa cgtggagaat ggctttcgat tgtagggcat aatggtagtg gtaaatcaac
     8521 gacggttcga ttaattgatg gcttactgga agcagaatcc ggagagattg taattgatgg
     8581 ccaacgtttg actgaggaaa atgtttggaa tatacgtcgt caaatcggta tggtttttca
     8641 aaatccagac aatcaatttg ttggagcgac tgttgaagat gatgttgctt ttggtttgga
     8701 aaatcaggga ctttctcgtc aagaaatgaa aaagagagtg gaagaagctc tggctttagt
     8761 tggcatgttg gactttaaaa agagagagcc agcgcgtcta tcaggtggcc aaaagcaacg
     8821 tgtggccatt gcaggtgttg tagccctaag accagctatt ttaatcttag atgaagcaac
     8881 gagtatgttg gatcctgagg ggcgtagaga acttattggg acagtaaaag gaattcgaaa
     8941 agactatgat atgacagtca tttctattac ccatgatttg gaagaagtcg ccatgagtga
     9001 tcgcgtattg gtcatgaaaa aaggggaaat tgaatcaact agtagtccaa gggagctttt
     9061 ctctcgaaat gatttagatc aaattggatt agacgatcct tttgctaatc aattaaaaaa
     9121 atctttgagc cagaatggct atgatttacc tgaaaattat ttgacagaaa gtgagctaga
     9181 ggataagcta tgggaattgc tctagaaaat gtgaatttta catatcaaga aggtactccc
     9241 ttagcttcag cagctttgtc ggatgtttct ttgacgattg aagatggctc ttatacagct
     9301 ttaattgggc acacaggtag tggtaaatca actattttac aactcttaaa tggtttattg
     9361 gtgccaagtc aagggagtgt gagggttttt gataccttaa tcacctcgac ttctaaaaat
     9421 aaagatattc gtcaaattag aaaacaggtt ggcttggtat ttcagtttgc tgaaaatcag
     9481 atttttgaag aaacggtttt gaaggacgtt gcttttggac cgcaaaattt tggagtttct
     9541 gaagaagatg ctgtgaagac tgcgcgtgag aaactggctc tggttggaat tgatgaatca
     9601 ctttttgatc gtagtccgtt tgagctgtca gggggacaaa tgagacgtgt tgccattgca
     9661 ggcatacttg ccatggagcc atctatatta gtcttagatg agccaacagc tggtctagat
     9721 cctctaggga gaaaagagtt gatgaccctg ttcaaaaaac tccaccagtc agggatgacc
     9781 atcgtcttgg taacgcattt gatggatgat gttgctgaat atgctaatca agtctatgta
     9841 atggaaaagg gacgtttagt aaaggggggc aaaccaagtg atgtctttca agacgttgtt
     9901 tttatggaag aagttcagtt gggagtacct aaaattacgg ccttttgtaa acgattggct
     9961 gatagaggcg tgtcatttaa acgattaccg attaagatag aggagttcaa ggagtcgcta
    10021 aatggatagt atgattttgg ggcgttatat cccaggggat tcgattgttc accgattgga
    10081 tccacgtagc aaattgcttg ctatgatgct actgattttg attgtgtttt gggctaataa
    10141 ccccttgacg aatctgattc tttttatagc gacagggata tttattgcct tgtcaggagt
    10201 atctctttca ttttttattc agggcttgaa gtctatgttt ttcttgattg ccttcacaac
    10261 tatttttcaa ctatttttca tttctaatgg gaatgtttta tttgagtttt cgtttgtgag
    10321 aatcacggat tatgctttgc aacaagctgg gattattttt tgtcgctttg tattgattat
    10381 tttcttttca actttgttaa ccttaacaac catgccttta agtttggcat cagctgtcga
    10441 agctttatta gcacctttaa agcgtgtgaa agttccagtt catgaaattg gattgatgct
    10501 gtctatgagt ttgcgttttg tcccaacctt gatggatgat acgacgcgga ttatgaatgc
    10561 acagaaagct cgtggagtgg attttggaga aggaagtatc gttcaaaaag taaaggcgat
    10621 gattcccatt ttgattcctc tttttgcgac aagtttaaaa cgtgcagatt ccttggctat
    10681 cgctatggaa gcgcgtggct atcagggtgg aaaaggcaga agtcaatacc gacaattgaa
    10741 atggactcta aaggatacgc tgaccattct tgttattctc gtacttggtt gttgtttatt
    10801 tttcttaaaa tcttagtagc tttcaggaat tgataaaaag ttactgtaac agtttttgat
    10861 ataaaggtag gaatatgaac cgttttaaaa aatcaaaata tgtcattatt gtttttgtca
    10921 ctgttctgct tgtgtcagct ctcttagcga cgacttattc aagtacaatt gtgacaaaat
    10981 taggagatgg aatctcattg gttgatagag ttgtacaaaa accttttcag tggtttgatt
    11041 ctgtcaaatc agatttggct catttgacac gaacatataa tgaaaatgaa agtttgaaga
    11101 aacagcttta ccaattagaa gttaaatcaa atgaggtgga aagtttaaag acagaaaatg
    11161 aacaactgcg ccaattgctt gatatgaagt ctaaattgca agccacaaag actttagcag
    11221 cagatgttat tatgcgttct ccggtatctt ggaagcagga gttgacctta gatgcaggta
    11281 gatcaaaagg tgcttctgag aacatgttag ctattgcaaa tggtggcttg attgggagtg
    11341 tttcaaaagt agaggagaac tctactatag tcaaccttct gacaaatacg gaaaatgctg
    11401 ataagatttc tgttaaaatt caacatggct ctactacaat ttatggaatt attattggct
    11461 atgacaagga aaatgacgtt cttaaaatta gccaattaaa tagtaatagc gatattagtg
    11521 cgggagataa ggtgactact ggtggattag gaaactttaa cgttgctgat attcctgttg
    11581 gtgaagtggt tgccacaatg catagtacag actatttgac acgagaagta actgttaaat
    11641 tgagtgcaga tactcataat gtagatgtga tagaattagt ggggaattca taatgagaca
    11701 gttgaagcga gttggagtat ttttattgct tcctttcttt gttctaattg acgcccatat
    11761 tagccagctt ctgggctcat ttttccccca tgtacatttg gctagtcatt ttctttttct
    11821 atttctctta tttgagacga tagaagtatc agagtatctc tacctagtct attgttttgt
    11881 tataggcttg gtttatgatg tttacttttt ccatctaata gggattacaa ctctcttatt
    11941 tatcttattg ggagccttcc ttcataaatt gaatagtgtt attttgttga atcgttggac
    12001 aagaatgcta gctatgattg tgctgacatt cctgtttgaa atgggtagtt atcttttggc
    12061 ttttatggta gggttgacag tagatagcat gtcgattttt atagtctata gcttggtacc
    12121 gacgatgatt ttaaattttt tatggattac tgtttttcaa tttatttttg aaaaatatta
    12181 tctataagaa cgacatataa atgtaacaaa ggcgtaatat ttattaggcc tttttttggt
    12241 atactagtat tgtctttaaa agaaggagta tctacgtaat atgaagaaaa aaatcttagc
    12301 gtcactttta ttaagtacag taatggtttc tcaagtagct gttttaacaa ctgcgcatgc
    12361 agaaacgact gatgacaaaa ttgctgctca agataataaa attagtaact taacagcaca
    12421 acaacaagaa gcccaaaaac aagttgacca aattcaggag caagtatcag ctattcaagc
    12481 tgagcagtct aacttgcaag ctgaaaatga tagattacaa gcagaatcta agaaactcga
    12541 gggtgagatt acagaacttt ctaaaaacat tgtttctcgt aaccaatcgt tggaaaaaca
    12601 agctcgtagt gctcaaacaa atggagccgt aactagctat atcaatacca ttgtaaactc
    12661 aaaatcaatt acagaagcta tttcacgtgt tgctgcaatg agtgaaatcg tatctgcaaa
    12721 caacaaaatg ttagaacaac aaaaggcaga taaaaaagct atttctgaaa aacaagtagc
    12781 aaataatgat gctatcaata ctgtaattgc taatcaacaa aaattggctg atgatgctca
    12841 agcattgact acgaaacagg cagaactaaa agctgctgaa ttaagtcttg ctgctgagaa
    12901 agcgacagct gaaggggaaa aagcaagtct attagagcaa aaagcagcag ctgaggcaga
    12961 ggctcgtgca gctgcggtag cagaagcagc ttataaagaa aaacgagcta gccaacaaca
    13021 atcagtactt gcttcagcaa acactaactt aacagctcaa gtgcaagcag tatctgaatc
    13081 tgcagcagca cctgtccgtg caaaagttcg tccaacatac agtacaaacg cttcaagtta
    13141 tccaattgga gaatgtacat ggggagtaaa aacattggca ccttgggctg gagactactg
    13201 gggtaatgga gcacagtggg ctacaagtgc agcagcagca ggtttccgta caggttcaac
    13261 acctcaagtt ggagcaattg catgttggaa tgatggtgga tatggtcacg tagcggttgt
    13321 tacagctgtt gaatcaacaa cacgtatcca agtatcagaa tcaaattatg caggtaatcg
    13381 tacaattgga aatcaccgtg gatggttcaa tccaacaaca acttctgaag gttttgttac
    13441 atatatttat gcagattaat ttacagaggg actcgaatag agccctcttt tcaggtttta
    13501 ccgtgacaat ccctattaaa aattatatca aaatagcttg aaaatattgg aaaagtatgg
    13561 tagaatgaaa attgtcgtgt gaacgataat actcattctt gatgaattgt gaagcagttg
    13621 cccttgggtc gttttgcgag ttgaagtcaa gaagaggaaa aaaacaaaaa ggagaaatac
    13681 tcatggcagt aatttcaatg aaacaacttc ttgaggctgg tgtacacttt ggtcaccaaa
    13741 ctcgtcgctg gaatcctaag atggctaagt acatctttac tgaacgtaac ggaatccacg
    13801 ttatcgactt gcaacaaact gtaaaatacg ctgaccaagc atacgacttc atgcgtgatg
    13861 cagcagctaa cgatgcagtt gtattgttcg ttggtactaa gaaacaagca gctgatgcag
    13921 ttgctgaaga agcagtacgt tcaggtcaat acttcatcaa ccaccgttgg ttgggtggaa
    13981 ctcttacaaa ctggggaaca atccaaaaac gtatcgctcg tttgaaagaa attaaacgta
    14041 tggaagaaga tggaactttc gaagttcttc ctaagaaaga agttgcactt cttaacaaac
    14101 aacgtgcgcg tcttgaaaaa ttcttgggcg gtatcgaaga tatgcctcgt atcccagatg
    14161 tgatgtacgt agttgaccca cataaagagc aaatcgctgt taaagaagct aaaaaattgg
    14221 gaatcccagt tgtagcgatg gttgacacca atactgatcc agatgatatc gatgtaatca
    14281 tcccagctaa cgatgacgct atccgtgctg ttaaattgat cacagctaaa ttggctgacg
    14341 ctattatcga aggacgtcaa ggtgaggatg cagtagcagt tgaagcagaa tttgcagctt
    14401 cagaaactca agcagattca attgaagaaa tcgttgaagt tgtagaaggt gacaacgctt
    14461 aatttataca aatagtaatt acctaggagg gcggggctta gcccggctct cctattttca
    14521 aaaaatatag gagaattaaa atggcagaaa ttacagctaa acttgtaaaa gagttgcgtg
    14581 aaaaatctgg tgccggtgtt atggacgcta aaaaagcgct tgtagaaaca gacggtgaca
    14641 tcgaaaaagc gattgaattg cttcgtgaaa aaggtatggc taaggcagct aagaaagctg
    14701 accgtgttgc tgcagaaggt ttgactggtg tttatgttaa cggtaatgtt gcagcagtta
    14761 ttgaagtaaa cgctgaaact gacttcgttg caaaaaacgc tcaattcgtt gaattggtaa
    14821 atactacagc taaagtcatt gctgaaggaa aacctgctaa caatgaagaa gctcttgctt
    14881 tgataatgcc ttcaggtgaa actcttgaag ctgcatacgt atctgcaaca gcaactatcg
    14941 gagagaaaat ctcattccgt cgctttgcat tgattgaaaa aacagacgca caacactttg
    15001 gagcatacca acataacggt ggacgtatcg gtgttatttc agttgttgaa ggtggagacg
    15061 aagcacttgc taaacaattg tcaatgcaca tcgcagcgat gaaaccaaca gttctttctt
    15121 acaaagaatt ggatgagcaa ttcgttaaag atgagttggc acaattgaat cacgttatcg
    15181 accaagacaa cgaaagccgt gcaatggtta ataaaccagc tcttccacac ttgaagtatg
    15241 gatcaaaagc tcaattaact gatgatgtta ttgctcaagc tgaagctgac atcaaagctg
    15301 aattggctgc agaaggcaaa ccagaaaaaa tctgggataa aattattcca ggtaaaatgg
    15361 atcgcttcat gcttgataac actaaagttg accaagctta cacacttctt gcacaagttt
    15421 acatcatgga tgacagcaag acagttgaag cataccttga atcagttaac gcttcggtag
    15481 ttgagtttgc tcgctttgaa gttggtgaag gtatcgagaa agctgcaaac gacttcgaag
    15541 ctgaagttgc agctacaatg gcagcagcct tgaataacta ataatagaaa ggaagcaatt
    15601 tgcttccttt tttgtatagg gcttatattt ttcctgaaat tgtttttccc ccagtctcac
    15661 tcgattagaa taattaaaac aaaaagccct ataataaggg ctaagtgcat ttaggagact
    15721 atacttcaaa ttcatagtaa gcgcgtcata acaaggtatc tatcctaatt ccccaacttc
    15781 cagtatacta gataaaaaat aattatcgga ggaaaggtat tgtactatga tgattcaact
    15841 aagtgattta ggtcaagttc acattgtttg tggcaagaca gatatgaggc agggaataga
    15901 ctcattagcc tatgtagtta aaacccactt tgaattggat tctttctccg gtcaagtctt
    15961 tctcttttgt ggtgaacgta aagaccgctt taaagccctt tactgggatg gtcaaggatt
    16021 ttggctacta tataaacgct ttgagaacgg aaaactgact tggctaagta cagaaaagga
    16081 tgtcaaagct ctcgcacctg aacaagtaga ttggcttatg aagggctttt ctatcactcc
    16141 aaaaatatag tagattgaaa ctagaatagt acacctctgc ttctaaaaca ttgttagaaa
    16201 tcgatttgat tgtcctgatc gatttgtcct gttcttattt cattttacta taaatccatc
    16261 agaaagtcgt gatttctatt gaaatgagga ctttcttttt atactcatct gctttcaaaa
    16321 agcattctag tccatctccg attaacgatg gactttatca cctccttctc cagtccttgt
    16381 ataacatctt ggagttgatt catgacatct tccaaagttt aaaaggcttt attcttaaat
    16441 ccacgtttac gaatctcttt ccacacttgt tcaatggggt tcatctctgg tgtgtatgga
    16501 ggaataaatg caaagccaat attagtcgga atctttaagg tacttgattt atgccatata
    16561 gcattgtcca taacgagtaa aagataatca tctggataag cttgtgaaat ctcctattcc
    16621 taaagcccct ttagcgcata actttggctc agcttctatt atcgctcaca ccatccatca
    16681 gaagtttaat ctgaaggtac ccaattatcg ccaagaagaa gattgggcta ggatgggttt
    16741 accaatcaca cgtaaggaaa tctctaatcg gcatatcaag acgagtcaat actatttgga
    16801 gcccctttat aacctcttgc gagagagact attgactcag cccttacttc atgcggatga
    16861 aacttcttat agggtgctag agagtgatag tcagctgact tactattgga cttttttgtc
    16921 tgggaaagct gagaaacaag ggattacgct ttaccatcat gtcctaatcg atttgttcat
    16981 atcttatttc aatccattat aaatagtgag aaatatctat cctatcttct agaatgtctt
    17041 ccaaacgtgg aaactctcgt aaacaaagag gttttagaga cctatttacc atggactaaa
    17101 gttgtacaag aaaagtgcaa ataagaaatc tccagattag gaactatccg tgagttctct
    17161 agtctggaga tttttcaata gacttcgtta ttggacggtt acaattcata aagtgctgta
    17221 gagagataac gttcaccgtt atctggtgct agggcaagga cttttttacc tgtacctaat
    17281 tttttggcaa cctcgatggc tccgtagata gctgcagctg aggaaatccc tacaaggaag
    17341 ccttcttttc caccaatttc acgtccgagt gcaagagcgt catctgatgt tacacgaacg
    17401 ataccatcat aggctttagt atcaagtgta tcaggaataa atccagctga gataccttga
    17461 attttgtgag gaccaggttt ttcaccagat agaatagcag attcatctgc ttctactgca
    17521 aaaacttgaa tgttagaatt ttctgatttg agtgcatgag aaacaccaga aatcgttcca
    17581 ccagtaccta ctccagcaac aaaggcatct aatccatctt taccgaaagc agctagtatc
    17641 tcagctcctg ttgttctttc gtgtacttct ggattagctg gattgtcaaa ttgaagagga
    17701 aggaaaccat cacgttcagc agcgatttct tgagccttag caatagcacc tttcattccc
    17761 tcgctaccag gagttaggac gagttcagca ccataagctt ggataatttt acgtcgttct
    17821 acactcatag tttcaggcat aacgatgacg actttatacc ctttagcagc acctacccat
    17881 gaaagtccaa taccggtgtt tccacttgtt gcttcaacaa tagtagaacc aagtttcaga
    17941 ataccatctt gttcagcttt ttcaatcatg ctaagggcaa tacggtcttt tacagatgaa
    18001 ccaggattaa atgcttcaag ctttatatag acgtctgcag caccttctgg cacgatgttg
    18061 ttaagtttaa caatcggtgt ttgaccgatt aattcagtaa tgttgttata aatagacata
    18121 tgaatacctc tttgtataag atatctttag tataacgcta tccataccat ttgtaaaata
    18181 tagaaatcct atcgaagcga taggatttct atatatcata gatcaaagtc attaattttc
    18241 aagtgattgc gttcagttaa ttctagtaaa taggtgtgaa ggaaaacgat atcaggtttc
    18301 gtttgaattg cttcattttc tagtatattt tggtacgcat ctttttattt tgctcgctaa
    18361 agtagttgtg agaatgtcaa cgaaacgctg tattattcat tgtttacact aagtttacag
    18421 gaacttcaac ctctcgtaaa ccttggtcag ttaaagtgac ttttccatta aaaaactcca
    18481 caagtgcagc tttaatagtt tctttttctt ctttatcaac ataaatcatc gtatcgactt
    18541 gatctgtaaa gtttgtatcc agctccatga gatcatgttc tttaaggaag ttactgtact
    18601 cttggtactg agcataagac atttgaatag caatgccagc ctgttctttt atttcaataa
    18661 taccaatttc tttgacagct aaggcgacac tgccggcgta agcacgaatt agtcctccag
    18721 cgcctagttt aataccacca aagtagcgtg tcacgaccac acagacattg gtgagattgt
    18781 gattttctag taccccaagc atgggaacac cagcagtacc actaggctca ccatcatcac
    18841 ttgtacgttt aatttcacta cgttctccaa taataaaggc agagcagtta tgtgtcgctt
    18901 tgtagtgttc ttttttgatg gcagtaatga agtcacgagc ctcttcttcg ctataaacac
    18961 gcttggcatg gcagataaag cgagattttt tgatttcttc ttggacttgg ccgtcctctt
    19021 taattgtcct aaattccatg atttaattgt actaaaaaat catctaaagt gctagttttt
    19081 acgaataaag aagtatgaaa gtaaatttag attatctcgg tcgtttattt actgagaatg
    19141 aattaacaga agaagaacgt cagttggcgg agaaacttcc agcaatgaga aaggagaagg
    19201 ggaaactttt ctgtcaacgt tgtaatagca ctattctaga agaatggtat ttgcccatcg
    19261 gtgcttacta ttgtcgagag tgcttgctga tgaagcgagt cagaagtgat caaactttat
    19321 actattttcc gcaggaggat tttccgaagc aagatgttct caaatggcgc ggccaattaa
    19381 ctccttttca agagaaggtg tcagagggac tgcttcaagc agtagacaag caaaagccaa
    19441 ccttagttca tgcggtaaca ggagctggaa agacagaaat gatttatcaa gtagtggcta
    19501 aagtgatcaa tgcgggtggt gcagtgtgtt tggctagtcc tcgcatagat gtttgtttgg
    19561 agctgtacaa gcgcctgcaa caggattttt cttgcgggat agctttgcta catggagaat
    19621 cggaacctta ttttcgaaca ccactagttg ttgcaacaac ccatcagtta ttgaagtttt
    19681 atcaagcttt tgatttgctg atagtggatg aagtagatgc ttttccttat gttgataatc
    19741 ccacgcttta ccatgctgtc aagaatagtg taaaggagaa tggattgaga atctttttaa
    19801 cagcgacttc gaccaatgag ttagataaaa aggtccgttt aggagaacta aaaagactga
    19861 atttaccgag acggtttcat ggaaatccgt tgattattcc aaaaccaatt tggttatcgg
    19921 attttaatcg ctacttagac aagaatcgtt tgtcaccaaa gttaaagtcc tatattgaga
    19981 agcagagaaa gacagcttat ccgttactca tttttgcttc agaaattaag aaaggggagc
    20041 agttagaaga aatcttacag gagcaatttc caaatgagaa aattggcttt gtatcttctg
    20101 taacagagga tcgattagag caagtacaag cttttcgaga tggagaactg acaatactta
    20161 tcagtacgac aatcttggag cgtggagtta ccttcccttg tgtggatgtt ttcgtagtag
    20221 aggccaatca tcgtttgttt accaagtcta gtttgattca gattggtgga cgagttggac
    20281 gaagcatgga tagaccgaca ggagatttgc ttttcttcca tgatgggtta aatgcttcaa
    20341 tcaagaaggc gattaaggaa attcagatga tgaataagga ggctggtcta tgaagtgctt
    20401 gttatgtggg cagactatga agactgtttt aacttttagt agtctcttac ttctgaggaa
    20461 tgatgactct tgtctttgtt cagactgtga ttctactttt gaaagaattg gggaagagaa
    20521 ctgtccaaat tgtatgaaaa cagagttgtc aacaaagtgt caagattgtc aactttggtg
    20581 taaagaagga gttgaagtca gtcatagagc gatttttact tacaatcaag ctatgaagga
    20641 ttttttcagt cggtataagt ttgatggaga cttcctgtta agaaaagttt tcgcttcatt
    20701 tttaagtgag gagttgaaaa agtacaaaga gtatcaattt gttgtaattc ccctaagtcc
    20761 tgatagatat gctaatagag gatttaatca ggttgagggc ttggtagagg cagcaggctt
    20821 tgagtatctg gatttattag agaaaagaga agagagagcc agttcttcta aaaatcgttc
    20881 agagcgcttg gggacagaac ttcctttctt tattaaaagt ggagtcacta ttcctaaaaa
    20941 aatcctactt atagatgata tctatactac aggagcaact ataaatcgtg ttaagaaact
    21001 gttggaagaa gctggtgcta aggatgtaaa aacattttcc cttgtaagat gaggaaaaaa
    21061 tttgcaaaaa tagaatgaaa gcgttataat aaaatcataa aaacaaaaat gtttagaaag
    21121 aaggtactca tatgattaaa tatagtatcc gtggtgaaaa cctagaagta acagaagcaa
    21181 ttcgtgatta tgtagtttct aaactcgaaa agatcgaaaa gtacttccaa ccagaacaag
    21241 agttggatgc ccgaattaac ttaaaagttt atcgtgaaaa aacggctaaa gtggaagtaa
    21301 cgattccgct tggatctatt actctccgcg cagaagatgt atctcaagat atgtatggtt
    21361 caattgacct tgtaactgat aaaattgaac gtcagattcg taaaaataaa acaaaaatcg
    21421 agcgtaaaaa taaaaataag gtagcaactg gtcaattatt tacagatgct ttggtggaag
    21481 attcaaatat tgtccagtct aaagttgttc gttcaaaaca aattgattta aaaccaatgg
    21541 atttggaaga agcaattcta caaatggatt tattggggca tgatttcttt atctatgtgg
    21601 atgttgaaga tcagacaacc aacgtgattt atcgtcgtga ggatggcgaa attggtttgt
    21661 tagaggttaa agaatcttaa tttcaatatg tgtaaaggta ggtttactga attgtaaact
    21721 acctttttct tataattgat agaattactg attacacttt taaaaagtcg ctttttgagg
    21781 ggtattatgg tataatgggt gccaagaggt tttgaatgaa aaaattttat gtaagtccaa
    21841 tttttcctat tctagtagga ttgattgcgt ttggagtctt atccactttc attatttttg
    21901 ttaataataa tctgttgacg gttttaattt tgtttctttt tgtaggaggc tatgtttttt
    21961 tatttaagaa actgagagtg cattatacaa ggagtgatgt agaacagata cagtatgtaa
    22021 accaccaagc ggaagaaagt ttgacagctc tattggaaca gatgcctgta ggtgttatga
    22081 aattgaattt atcttctgga gaggttgagt ggtttaatcc ctatgctgaa ttgattttga
    22141 ccaaggaaga tggtgatttt gatttagaag ctgttcaaac gattatcaag gcttcagtag
    22201 gaaatccgtc tacttatgcc aagcttggtg agaagcgtta tgctgttcat atggatgctt
    22261 cttccggtgt tttgtatttt gtagatgtat ccagggaaca agccataaca gatgaattgg
    22321 taacaagtcg accagtgatt gggattgtct ctgtggataa ttatgatgat ttggaggatg
    22381 caacttctga gtcagatatt agtcaaatca atagttttgt agctaatttt atatcagagt
    22441 tttcagaaaa atacatgatg ttttctcgtc gggtaagtat ggatcgattt tatctattta
    22501 ctgactacac ggtgcttgag ggcttgatga atgataaatt ttctgttatt gatgctttca
    22561 gagaagagtc gaaacagaga cagttgccct tgaccttaag tatgggattt tcttatggcg
    22621 atggaaatca tgatgagata gggaaagttg ctttgctcaa tttgaacttg gctgaagtac
    22681 gtggtggcga ccaggtggtt gttaaggaaa acgacgaaac gaaaaatcca gtttattttg
    22741 gtggtgggtc tgctgcttca atcaagcgta cacggactcg tacgcgcgct atgatgacag
    22801 ctatttcaga taagattcgg agtgtagatc aggtttttgt agtcggtcac aaaaatttag
    22861 acatggatgc tttgggctct gctgtaggta tgcagttgtt cgccagcaat gtgattgaaa
    22921 atagctatgc tctttatgat gaagaacaaa tgtctccaga tattgaacga gctgtttcat
    22981 tcatagaaaa agaaggagtt acgaagttgt tgtctgttaa ggatgcaatg gggatggtga
    23041 ccaatcgttc tttgttgatt cttgtagacc attcaaagac agccttaaca ttatcaaaag
    23101 aattttatga tttatttacc caaaccattg ttattgacca ccatagaagg gatcaggatt
    23161 ttccagataa tgcagtcatt acttatatcg aaagtggtgc aagtagtgcc agtgagttgg
    23221 taacggaatt gattcagttc cagaattcta agaaaaatcg tttgagtcgt atgcaagcaa
    23281 gtgtcttgat ggctggtatg atgttggata ctaaaaattt cacctcgcga gtaactagtc
    23341 ggacatttga tgttgctagc tatctcagaa cgcgcggaag tgatagtatt gctatccagg
    23401 aaatcgctgc gacagatttt gaagaatatc gtgaggtcaa tgaacttatt ttacaggggc
    23461 gtaaattagg tttagatgta ctaatagcag aggctaagga cacgaaatgc tatgatacag
    23521 ttgttattag taaggcagca gatgccatgt tagccatgtc aggtattgaa gcgagttttg
    23581 ttcttgcgaa gaatacacaa ggatttatct ctatctcagc tcgaagtcgt agtaaactga
    23641 atgtacaacg gattatggaa gagttaggcg gtggaggcca ctttaatttg gcagcagctc
    23701 aaattaaaga tgtaaccttg tcagaagcag gtgaaaaact gacagaaatt gtattaaatg
    23761 aaataaagga aaaggagaaa gaagaatgaa agtaatcttt ttagcagatg ttaaaggaaa
    23821 aggtaaaaaa ggcgaaatta aggaagtacc aacagggtat gcgcaaaact ttcttatcaa
    23881 aaagaatcta gccaaagaag cgactgctca agctgtaggt gaacttcgtg gtaaacaaaa
    23941 atcggaagaa aaagctcacg ctgagatgat tgcagaagga aaagcaatta aagcacaact
    24001 tgaagcagaa gaaactgttg tagaatttgt tgaaaaagtt ggtccagatg gtcgtacctt
    24061 tggttctatt accaataaga agattgcaga agaattgcaa aagcaatttg gaattaagat
    24121 tgataaacgt catattcaag tacaagctcc gattcgagcg gttggtttga ttgatgtgcc
    24181 agtgaaaatc tatcaagata tcacaagtgt aatcaatctt cgtgtgaaag aaggataagt
    24241 ttacaccttc ttgacaagat tgtaaaagga agggaagtct gatggcagaa gtagaagagt
    24301 tacgagtaca acctcaagat atcttagctg agcaatccgt tttaggggct atctttattg
    24361 atgagagtaa acttgttttt gtgcgagaat acattgagtc tcgggacttt tttaagtatg
    24421 cccatcgttt gattttccaa gccatggtcg atttatccga tcgtggtgat gccatagatg
    24481 caacaacggt tcgtactatc cttgataatc aaggtgattt acagaatatt ggtggcttgt
    24541 cttacttggt tgagattgtt aattctgtgc caacttctgc taatgcggag tattatgcta
    24601 agattgttgc agaaaaagca atgctacgtc gtttaattgc caagttgaca gagtctgtca
    24661 accaagctta cgaagcgtca caaccagctg atgaaattat tgctcaggca gaaaaagggt
    24721 tgattgatgt cagtgaaaat gcaaatcgaa gcgggtttaa gaacattcga gatgtgttga
    24781 atctcaactt tggaaatctg gaagctcgct cgcaacaaac gaccgatatt acaggtattg
    24841 cgacaggtta tcgtgatttg gatcatatga caacaggact tcatgaggag gagttgatta
    24901 tcttagcagc tcgtccagca gttggtaaga cagcatttgc cttgaatatc gctcagaaca
    24961 ttgggactaa gttggacaaa acggttgcta ttttttcact cgaaatgggt gcggaaagct
    25021 tggtagaccg tatgttagct gcagaaggct tggtggagtc acattctatc cgtacagggc
    25081 aattgacaga tgaggagtgg caaaaatata ctattgctca gggtaatcta gctaacgcca
    25141 gtatctatat cgatgatacg ccaggtattc ggattacaga gattcgttct cgttctcgta
    25201 aattggctca agaaactgga aatcttggtt tgattttgat agactatttg caacttatca
    25261 cgggaactgg tcgagaaaat cgtcaacaag aagtttctga aatttctcgt cagttgaaaa
    25321 tactagccaa ggaattgaag gttccagtaa tcgctctgag tcagctttct cgtggtgtag
    25381 aacaacgtca ggacaagaga ccggtcttgt ctgatattcg tgaatctggg tctattgagc
    25441 aggacgctga tatcgtagct tttctctatc gcgatgacta ctatgaacgt ggtggtgaag
    25501 aagaggaggg tatcccaaat aataaggtgg aagttattat cgagaaaaac cgtagtggag
    25561 ctcgtggaac agtggaattg attttccaaa aagaatacaa taaattttca agtatctcaa
    25621 agagggaggc ataagatgtc agatgcattt acagatgtag ccaagatgaa aaaaatcaaa
    25681 gaagaaatca aggcacatga gggacaagtc gtagaaatga ctttggagaa tggtcgtaag
    25741 cgccaaaaaa atagattggg taagctaatt gaagtttatc catctctatt tattgtggag
    25801 tttgggaatg tggaaggaga taaacaagtt aatgtttacg ttgaatcctt tacttactca
    25861 gatattctta cagaaaagaa tttgattcat tatcttgact aaagtgagaa attttctcac
    25921 tttttctttt ttctccgaat aatttaggtg aaggcaatca tcgctttata ttatttttca
    25981 aggaggaaga atgaaaattt taccgtttat agcaagaggg acaagttatt acttgaagat
    26041 gtcagttaaa aagcttgttc cttttttagt agtaggattg atgctagcag ctggtgatag
    26101 tgtctatgcc tattccggag gaaatggatc gattgcgcgt ggggatgatt atcctgctta
    26161 ttataaaaat gggagccagg agattgatca gtggcgcatg tattctcgtc agtgtacttc
    26221 ttttgtagcc tttcgtttga gtaatgtcaa tggttttgaa attccggcag cttatggaaa
    26281 tgcgaatgaa tggggacatc gtgctcgtcg ggaaggttat cgtgtagata atacaccgac
    26341 gattggttcc attacttggt ctactgcagg aacttatggt catgttgcct gggtgtcaaa
    26401 tgtaatggga gatcagattg agattgagga atataactat ggttatacag aatcctataa
    26461 taaacgagtt ataaaagcaa acacgatgac aggatttatt cattttaaag atttggatgg
    26521 tggcagtgtt gggaatagtc aatcctcagc ttcaacaggc ggaactcatt attttaagac
    26581 caagtctgct attaaaactg aacccctagt tagtgcaact gtgattgatt actattatcc
    26641 tggggagaag gttcattatg atcagatact tgaaaaagac ggctataagt ggttgagtta
    26701 tactgcctat aatggaagct atcgttatgt tcaattggag gctgtgaata aaaatcctct
    26761 aggtcattct gttctttctt caacaggtgg aactcattat tttaagacca agtctgctat
    26821 caaaactgaa cccctagtta gcggaactgt gattgattac tattatcctg gggagaaggt
    26881 tcattatgat cagatacttg aaaaagacgg ctacaagtgg ttgagttata ctgcctataa
    26941 tggaagtcgt cgctatatac agctagaggg agtgacttct tcacaaaatt atcagaatca
    27001 atcaggaaat atctctagct atggatccaa taatagttca actgtcggtt ggaagaaaat
    27061 aaatggtagt tggtatcatt tcaaatcaaa tggttctaaa tcaacaggat ggctgaaaga
    27121 cggttctagc tggtattatt tgaaattatc tggtgaaatg cagacaggat ggttaaagga
    27181 aaatggtttg tggtattatc tgggtagttc aggggcaatg aaaacaggct ggtaccaggt
    27241 ctctggtaag tggtattatt cttactcttc aggcgcctta gctgttaata cgacggtgga
    27301 tggctacaga gtaaacagtg atggagaacg agtatagaaa attggagtag gagaaatttc
    27361 ctgctctttt ctgtaagcag tttccttgac atttttctta ttttgcgcta tcatatatac
    27421 atataatata tgggagtctg tgtacagtct gagaggaagt gttaaacttc gaccgcacct
    27481 gatctgggta atgccagcgg agggaacgat acttagtcta attttgcacc ttttccatgt
    27541 atggtaaagg tttttctttt tttaaaaagg aaaacgagaa gaggaggttc ttatgaaagc
    27601 aagcattgcc ttgcaagttt tacccctagt acaggggatt gaacggatag ctgttattga
    27661 tcaggtcatt gcttatctgc aaactcaaga agtgacgatg gtagtgacac catttgaaac
    27721 ggtcttggaa ggggagtttg atgagcttat gcgcattcta aaagaagcgc tggaagtggc
    27781 agggcaggag gcagacaatg tctttgccaa tgtcaaaata aatgtaggag agattttaag
    27841 tattgatgag aaacttgaga agtatactga gacgacacat tagtctattg ggctttatcg
    27901 gagtattgtc aatctggcag ttagcaggtt ttcttaaact tctccccaag tttatcctgc
    27961 cgacacctct tgaaattctc cagccctttg ttcgtgacag agaatttctc tggcaccata
    28021 gctgggcgac cttgagagtg gctttactgg ggctgatttt gggagttttg attgcctgtc
    28081 ttatggctgt gctcatggat agtttgactt ggctcaatga cctgatttac cctatgatgg
    28141 tggtcattca gaccattccg accattgcca tagctcctat cctggtcttg tggctaggtt
    28201 atgggatttt gcccaagatt gtcttgatta tcttaacgac aacctttccc atcatcgtca
    28261 gcattttgga cggttttagg cattgcgaca aggatatgct gaccttgttt agtctgatgc
    28321 gggccaagcc ttggcaaatc ctgtggcatt ttaaaattcc agtcagcctg ccctactttt
    28381 atgctggtct gagggtcagt gtctcctacg cttttatcac aacagtggta tctgagtggt
    28441 tgggaggctt tgaggggctt ggtgtttata tgattcagtc caagaaattg tttcagtatg
    28501 ataccatgtt cgctattatt attctggtat cgattatcag ccttttgggt atgaagttgg
    28561 tcgatattag tgaaaaatat gttattaaat ggaaacgttc gtagaattag aatgtttctg
    28621 aaaaagaaaa gaggaaatca aaatgaagaa aacatggaaa gtgtttttaa cgcttgtaac
    28681 agctcttgta gctgttgtgc ttgtggcctg tggtcaagga actgcttcta aagacaacaa
    28741 agaggcagaa cttaagaagg ttgactttat cctagactgg acaccaaata ccaaccacac
    28801 agggctttat gttgccaagg aaaaaggtta tttcaaagaa gctggagtgg atgttgattt
    28861 gaaattgcca ccagaagaaa gttcttctga cttggttatc aacggaaagg caccatttgc
    28921 agtgtatttc caagactaca tggctaagaa attggaaaaa ggagcaggaa tcactgccgt
    28981 tgcagctatt gttgaacaca atacatcagg aatcatctct cgtaaatctg ataatgtaag
    29041 cagtccaaaa gacttggttg gtaagaaata tgggacatgg aatgacccaa ctgaacttgc
    29101 tatgttgaaa accttggtag aatctcaagg tggagacttt gagaaggttg aaaaagtacc
    29161 aaataacgac tcaaactcaa tcacaccgat tgccaatggc gtctttgata ctgcttggat
    29221 ttactacggt tgggatggta tccttgctaa atctcaaggt gtagatgcta acttcatgta
    29281 cttgaaagac tatgtcaagg agtttgacta ctattcacca gttatcatcg caaacaacga
    29341 ctatctgaaa gataacaaag aagaagctcg caaagtcatc caagccatca aaaaaggcta
    29401 ccaatatgcc atggaacatc cagaagaagc tgcagatatt ctcatcaaga atgcacctga
    29461 actcaaggaa aaacgtgact ttgtcatcga atctcaaaaa tacttgtcaa aagaatacgc
    29521 aagcgacaag gaaaaatggg gtcaatttga cgcagctcgc tggaatgctt tctacaaatg
    29581 ggataaagaa aatggtatcc ttaaagaaga cttgacagac aaaggcttca ccaacgaatt
    29641 tgtgaaataa tgacagaaat tagactagag cacgtcagtt atgcctatgg tcaggagagg
    29701 attttagagg atatcaacct acaggtgact tcaggcgaag tggtttccat cctaggccca
    29761 agtggtgttg gaaagaccac cctctttaat ctaatcgctg ggattttaga agttcagtca
    29821 gggagaattg tccttgatgg tgaagaaaat cccaaggggc gcgtgagtta tatgttgcaa
    29881 aaggatctgc tcttggagca caagacggtg cttggaaata tcattctgcc cctcttgatt
    29941 caaaaggtgg ataaggcaga agctatttcc cgagcggata aaattcttgc gaccttccag
    30001 ctgacagctg taagagacaa gtatcctcat gaacttagcg gtgggatgcg ccagcgtgta
    30061 gccttactcc ggacctacct ttttgggcac aagctctttc tcttagatga ggcctttagc
    30121 gccttggatg agatgacaaa gatggaactc cacgcttggt atcttgagat tcacaagcag
    30181 ttgcagctaa caaccctgat catcacgcat agtattgagg aggccctcaa tctcagcgac
    30241 cgcatctata tcttgaaaaa tcgccctggg cagattgttt cagaaattaa actagattgg
    30301 tctgaagatg aggacaagga agtccaaaag attgcctata aacgtcaaat cttggcagaa
    30361 ttaggcttag ataagtagaa aaatagggag ttggtgaaga ttatccttta ccagcgccct
    30421 ttttctttta aaaatgagaa aatttcggta taatagtcaa acaaggtcaa ggtttaaaga
    30481 gagaggtggg tttgttatga gatttaaaaa tacatcggat catattgagg cctacatcaa
    30541 ggcgatttta gatcaatctg gtatcgtgga gttgcaacgg agtcagttgg cagatacctt
    30601 tcaggttgtt cctagtcaga ttaactacgt gatcaagaca cgctttacgg aaagtagagg
    30661 ctacttggtt gaaagtaagc gtggtggcgg aggctacatt cgtataggac ggattgagtt
    30721 ttctagtcat catgaaatgc tccgggagct gctttactcg attggtgagc gagtcagtca
    30781 agaaatttat gaggatattc tccagctttt ggttgagcag gaattgatga ccaagcagga
    30841 gatgaatttg ctagaatcag tagctttgga tcgcgtttta ggagaagaag ctccagttgt
    30901 tcgagcaaac atgctacgtc aggtcataca agaggtagat agaaaaggga agtaagatga
    30961 actattcaaa agcattgaat gaatgtatcg aaagtgccta catggttgct ggacattttg
    31021 gagctcgtta tctagagtcg tggcacttgt tgattgccat gtctaatcac agttatagtg
    31081 tagcaggggc aactttaaat gattatccgt atgagatgga ccgtttagaa gaggtggctt
    31141 tggaactgac tgaaacggac tatagccagg atgaaacctt tacggaattg ccgttctccc
    31201 gtcgtttgca ggttcttttt gatgaagcag agtatgtagc gtcagtggtc catgctaagg
    31261 tactagggac agagcacgtc ctctatgcga ttttgcatga tggcaatgcc ttggcgactc
    31321 gtatcttgga gagggctggt ttttcttatg aagacaagaa agatcaggtc aagattgctg
    31381 ctcttcgtcg aaatttagaa gaacgggcag gctggactcg tgaagatctc aaggctttac
    31441 gccaacgcca tcgtacagta gctgacaagc aaaattctat ggccaatatg atgggcatgc
    31501 cgcagactcc gagtggtggt ctcgaggatt atacgcatga tttgacagag caagcgcgtt
    31561 ctggcaagtt agaaccagtc atcggtcggg acaaggaaat ctcacgtatg attcaaatct
    31621 tgagccgtaa gactaagaac aaccctgtct tggttgggga tgctggtgtc gggaaaacag
    31681 ctctggccct tggtcttgcc cagcgtattg ctagtggtga cgtgcctgcg gaaatggcta
    31741 agatgcgcgt gttagaactt gatttgatga atgtcgttgc agggacacgc ttccgtggtg
    31801 actttgaaga acgcatgaat aatatcatca aggatattga agaagatggc caagtcatcc
    31861 tctttatcga tgaactccac accatcatgg gttctggtag cgggattgat tcgactctgg
    31921 atgcggccaa tatcttgaaa ccagccttgg cgcgtggaac tttgagaacg gttggtgcca
    31981 ctactcagga agaatatcaa aaacatatcg aaaaagatgc ggcactttct cgtcgtttcg
    32041 ctaaagtgac gattgaagaa ccaagtgtgg cagatagtat gactatttta caaggtttga
    32101 aggcgactta tgagaaacat caccgtgtac aaatcacaga tgaagcggtt gaaacagcgg
    32161 ttaagatggc tcatcgttat ttaaccagtc gtcacttgcc agactctgct atcgacctct
    32221 tggacgaagc agcagcaaca gtgcaaaata aggcaaagca tgtaaaagca gacgattcag
    32281 atttgagtcc agctgacaag gccctgatgg atggcaagtg gaaacaggca gcccagctaa
    32341 tcgcaaaaga agaggaagta cctgtctaca aagacttggt gacagagtct gatattttga
    32401 ccaccttgag tcgcttgtca ggaatcccag ttcaaaaact gactcaaacg gatgctaaga
    32461 agtatttaaa tcttgaagca gaactccata aacgtgtcat cggtcaagat caagctgttt
    32521 caagcattag tcgtgccatt cgccgtaatc agtcaggaat tcgcagtcac aagcgtccga
    32581 tcggttcctt tatgttccta ggacccacag gtgtcggtaa gaccgagttg gccaaggctt
    32641 tggcagaagt tctctttgat gacgaatcag cccttatccg ctttgatatg agtgagtata
    32701 tggagaaatt cgcagccagc cgtctaaatg gagctcctcc aggctatgtg ggttacgaag
    32761 aaggtgggga gctgacagag aaggttcgca ataaacccta ttccgttctc ctctttgatg
    32821 aggtagagaa ggcccaccca gatatcttta atgttctctt gcaggttctg gatgacggtg
    32881 tcttgacaga tagcaaggga cgcaaggtcg atttttcaaa taccattatc attatgacat
    32941 cgaatctagg tgcgactgcc cttcgtgatg ataagactgt tggttttggg gctaaggata
    33001 ttcgttttga ccaggaaaat atggaaaaac gcatgtttga agaactgaaa aaagcttata
    33061 gaccggaatt catcaaccgc attgatgaga aggtggtctt ccatagccta tctagtgacc
    33121 atatgcagga agtggtgaag attatggtca agcctttagt ggcaagtttg gctgaaaaag
    33181 gcattgactt gaaattacaa gcttcagctc tgaaattgtt agcaaatcaa ggatatgacc
    33241 cagagatggg agctcgccca cttcgcagaa ccctgcaaac agaagtggag gacaagttgg
    33301 cagaacttct tcttaaggga gatttagtgg caggcaacac acttaagatt ggtgtcaaag
    33361 caggccagtt aaaatttgat attgcataaa agaataaaag tatcagcatc tgaccataag
    33421 tcacagtgga gtgaaactca atgaaaatca aagagcaaac taggcagcta gccgcaggtt
    33481 gctcaaaaca ctgttttgag gttgcagata gagctgacgt ggtttgaaga gattttcgaa
    33541 gagtatgaaa ctaaaaccta tagcttctaa acgattcgtg gttttcacca ttcaacacaa
    33601 aattcatatg tttattaccc tccgtcgtat ttgtcttaga gcatgtgtag tagaaaaaga
    33661 gcagtcttat ctgaaatttt tattctttca aaagagacct gtttcttttt tgcatgtcaa
    33721 atccgttcta gctggtattt aaaaaatcaa actaatattc aatgaaaatc aaagaacaaa
    33781 ctaggcagct agccgcaggt tgctcaaaac actgttttga ggttgtagat agagctgacg
    33841 tggtttgaag agattttcga agagtataag ctgcaagatg aatgattttc ttgtattgac
    33901 gttgttgttg acaaaaagta gcggataaat gaaatccatt ccattatcat agatgataga
    33961 ctggtaggaa attttcaaat agcatacagg aaatagatgt atggagttct ggtagtagaa
    34021 agggagagag atgaacattt tagttgcaga tgacgaggaa atgattagag aaggaattgc
    34081 agcatttctg acagaagagg gttatcatgt cattatggct aaggatggac aagaggtctt
    34141 ggaaaaattt caagatctcc ctatccatct catggtactg gatttaatga tgcctaggaa
    34201 gagtggtttt gaagtgttaa aagaaatcaa tcaaaagcac gatattcctg tcatcgtctt
    34261 gagtgctctg ggagatgaaa ctactcagtc acaggtattt gatctctatg ctgatgatca
    34321 tgtgacaaaa cctttttctt tggtactgct tgtcaagcgt attaaagcgc ttatcagacg
    34381 ttactacgtc atagaggata tttggcgata tcaggatgta acagtggatt ttacctctta
    34441 caaagcacat tataaaaatg aagaaattga tctcaaacca aaggaattac tggtactaaa
    34501 gtgtttgatt cagcataaaa atcaagtttt aagtagagag cagatattgg aagaaatttc
    34561 aaaagatgta gctgatttac cttgtgatag ggtcgttgat gtctatattc gtactcttcg
    34621 caaaaaatta gctttagatt gtatcgtgac tgtgaaaaat gttgggtata agattagctt
    34681 atgataaaaa atcctaaatt attaaccaag tcttttttaa gaagttttgc aattctaggt
    34741 ggtgttggtc tagtcattca tatagctatt tatttgacct ttccttttta ttatattcaa
    34801 ctggaggggg aaaagtttaa tgagagcgca agagtgttta cggagtattt aaagactaag
    34861 acatctgatg aaattccaag cttactccag tcttattcaa agtccttgac catatctgct
    34921 caccttaaaa gagatattgt agataagcga ctccctcttg tgcatgactt ggatattaaa
    34981 gatggaaagc tatcaaatta tatcgtgatg ttagatatgt ctgttagtac agcagatggt
    35041 aaacaggtaa ccgtgcaatt tgttcacggg gtggatgtct acaaagaagc aaagaatatt
    35101 ttgcttttgt atctcccata tacatttttg gttacaattg ctttttcctt tgttttttct
    35161 tatttttata ctaaacgctt gctcaatcct cttttttaca tttcagaagt gactagtaaa
    35221 atgcaagatt tggatgacaa tattcgtttt gatgaaagta ggaaagatga agttggtgaa
    35281 gttggaaaac agattaatgg tatgtatgag cacttgttga aggttattca tgagttggaa
    35341 agtcgtaatg agcaaattgt aaaattgcaa aatcaaaagg tttcctttgt ccgcggagca
    35401 tcacatgagt tgaaaacccc tttagccagt cttagaatta tcctagagaa tatgcagcat
    35461 aatattggag attacaaaga tcatccaaaa tatattgcaa agagtataaa taagattgac
    35521 cagatgagcc acttattgga agaagtactg gagtcttcta aattccaaga gtggacagag
    35581 tgtcgtgaga ccttgactgt taagccagtt ttagtagata ttttatcacg ttatcaagaa
    35641 ttagctcatt caataggtgt tacaattgaa aatcaattga cagatgctac cagggtcgtc
    35701 atgagtctta gggcattgga taaggttttg acaaacctga ttagtaatgc aattaaatat
    35761 tcagataaaa atgggcgcgt aatcatatcc gagcaagatg gctatctctc tatcaaaaat
    35821 acatgtgcgc ctctaagtga ccaagaacta gaacatttat ttgatatatt ctatcattct
    35881 caaatcgtga cagataagga tgaaagttcc ggtttgggtc tttacattgt gaataatatt
    35941 ttagaaagct atcaaatgga ttatagtttt ctcccttatg aacacggtat ggaatttaag
    36001 attagcttgt agacagatta gttttttatt aaagttcata tagggttaac ataagtgtgt
    36061 tattctttgt gtagataaaa gaaaggatac taatatggta ttagcgatta ttttagtaac
    36121 attctttatt cgattgattt ttttaaagcg ttcgatagag aatgagaaac gaatccttag
    36181 caatggcggg aaagaatttg gagttgagaa tacaaaacga ttaactatgg ctcatattgt
    36241 tttttatctc tcttgcttgg ttgaggcaat ggtgcacaag acaatttttg atagcatggg
    36301 catggttggt ttagtcttgc ttattttttc tatgctgatg ttaatgttgg tgattcactt
    36361 gttgggagat atttggacag tgaagcttat gcttgtcaat aatcacaaat atgtagatca
    36421 tatcttgttt aggacagtaa aacatcctaa ttacttttta aatattcttc ctgagttgat
    36481 tggcttgacc ttgttgagtc atgcttatgt gacttttgtt ttagtttttc cagtttatgc
    36541 agttattttg tatcgacgaa tagctgaaga ggaaaagcta ttacatgaag ttataatccc
    36601 aaatggaaga atgaaaggtt aaaaataaaa tacccaattt aatatacagt tcatattgga
    36661 agggtatact gaagatgaat aaaatattat aggatatcga tatgtttaaa tcaaattatg
    36721 aaagaaaaat gtgctattct attcgaaaat ttagtatagg agtagctagt gtagctgttg
    36781 ccagtcttgt tatgggaagt gtggttcatg cgacagagaa cgagggaact acccaagcac
    36841 ccacttcttc taatagggga aatgaaagtc aggcagaaca acgtagagaa ctcgatttag
    36901 aacgagataa ggtaaagaaa gaggtcaggg aatataaaga aaaaaaagtg aaagagctct
    36961 attcaaaatc aactaaaagt cgacataaga agactgtaga tatagttaac aagttgcaaa
    37021 acattaataa cgagtatttg aataaaataa ttcaatcaac ctcaacatac gaagaactac
    37081 agaaactgat gatggagagt caatcagaag tagataaagc tgtgtctgaa tttgaaaagg
    37141 acttatcttc ttcgtcaagt tcaggctctt ccacggaacc ggaagcttca gatacagcga
    37201 agccaaacaa gccgacagaa ctagaaaaaa aggtagcaga agctcagcag aaggttgaag
    37261 aagctgagaa aaaagccaag gatcaaaaag aagaagatca ccgtaactac ccaaccatta
    37321 cttacaaaac gcttgaactt gaaattgctg agttcgatgt gaaagttaaa gaagcggagc
    37381 ttgaactatt aaaagtgaaa gctaaggaat ctcgagacga gaaaaaaatt aagcaagcag
    37441 aagcggaagt tgagagtaaa caagctgagg ctacaaggtt aaaaaaaatc aagacagatc
    37501 gtaaaaaagc agaagaagaa gctaagttga aggaagctgt tgaaaagaat gcagcgactt
    37561 cagagcaagg taaaccaaag aggcgggtaa aacgaggagc tcttggagag caagcaacac
    37621 ctgataaaaa agattatttt gaaaaagact tccgtccagc tttcaataaa aaccagcaga
    37681 tggtagccat tcaagaatcc ttgaacaaac tagatgatga aacaaaaact gttccagatg
    37741 gggctaaact cacaggagaa gctggaaatg cctataatga ggtcagagat tatgcaataa
    37801 aagttgtttc tgaaaacaag aaacttctat cacagacagc agtgacaatg gatgaactgg
    37861 caatgcaatt aaccaaattg aacgatgcca tgtctaaatt gaaagaagct aaagcgaaat
    37921 tggtaccaga ggttaaaccg cagccggaaa atccagagca tcaaaaacca acaactccag
    37981 ctccggatac caaaccaatc cctcaaccag agggtaagaa accaagcgta ccagatatta
    38041 atcaggaaaa agaaaaagct aagcttgctg tagtaaccta catgagcaag attttagatg
    38101 atatacaaaa acatcatctg cagaaagaaa aacatcgtca gattgttgct cttattaagg
    38161 agcttgatga gtttaaaaag caagctcttt ctgaaattga taatgtaaat accaaagtag
    38221 aaattgaaaa tacagtccac aagatatttg cagacatgga tgcagttgtg actaaattca
    38281 aaaaaggctt aactcaggac acaccaaaag aaccagataa
//
LOCUS       DAVHIT010000009        36412 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVHIT010000009 DAVHIT010000000
VERSION     DAVHIT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.009"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(45..893)
                     /locus_tag="VVN16_000358"
     CDS             complement(45..893)
                     /locus_tag="VVN16_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5571807.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1151..1429
                     /locus_tag="VVN16_000359"
     CDS             1151..1429
                     /locus_tag="VVN16_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571808.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1431..3494
                     /locus_tag="VVN16_000360"
     CDS             1431..3494
                     /locus_tag="VVN16_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5571809.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3572..4828)
                     /locus_tag="VVN16_000361"
     CDS             complement(3572..4828)
                     /locus_tag="VVN16_000361"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5571810.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            4970..7435
                     /locus_tag="VVN16_000362"
     CDS             4970..7435
                     /locus_tag="VVN16_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU5571811.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7500..8411
                     /locus_tag="VVN16_000363"
     CDS             7500..8411
                     /locus_tag="VVN16_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5571812.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8641..9339
                     /gene="dapD"
                     /locus_tag="VVN16_000364"
     CDS             8641..9339
                     /gene="dapD"
                     /locus_tag="VVN16_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU5571813.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVKI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9407..10537
                     /locus_tag="VVN16_000365"
     CDS             9407..10537
                     /locus_tag="VVN16_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU5571814.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10549..11088
                     /locus_tag="VVN16_000366"
     CDS             10549..11088
                     /locus_tag="VVN16_000366"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU5571815.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11072..11749
                     /locus_tag="VVN16_000367"
     CDS             11072..11749
                     /locus_tag="VVN16_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5571816.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(11821..12720)
                     /gene="galU"
                     /locus_tag="VVN16_000368"
     CDS             complement(11821..12720)
                     /gene="galU"
                     /locus_tag="VVN16_000368"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU5571817.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12742..13758)
                     /locus_tag="VVN16_000369"
     CDS             complement(12742..13758)
                     /locus_tag="VVN16_000369"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU5571818.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            13970..14350
                     /locus_tag="VVN16_000370"
     CDS             13970..14350
                     /locus_tag="VVN16_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5571819.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(14524..14949)
                     /locus_tag="VVN16_000371"
     CDS             complement(14524..14949)
                     /locus_tag="VVN16_000371"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5571820.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(15017..15667)
                     /gene="phoU"
                     /locus_tag="VVN16_000372"
     CDS             complement(15017..15667)
                     /gene="phoU"
                     /locus_tag="VVN16_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5571821.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(15682..16434)
                     /gene="pstB"
                     /locus_tag="VVN16_000373"
     CDS             complement(15682..16434)
                     /gene="pstB"
                     /locus_tag="VVN16_000373"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5571822.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16436..17251)
                     /gene="pstA"
                     /locus_tag="VVN16_000374"
     CDS             complement(16436..17251)
                     /gene="pstA"
                     /locus_tag="VVN16_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5571823.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17244..18107)
                     /gene="pstC"
                     /locus_tag="VVN16_000375"
     CDS             complement(17244..18107)
                     /gene="pstC"
                     /locus_tag="VVN16_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5571824.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18225..19100)
                     /locus_tag="VVN16_000376"
     CDS             complement(18225..19100)
                     /locus_tag="VVN16_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU5571825.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19267..20598)
                     /locus_tag="VVN16_000377"
     CDS             complement(19267..20598)
                     /locus_tag="VVN16_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU5571826.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFNPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20599..21306)
                     /locus_tag="VVN16_000378"
     CDS             complement(20599..21306)
                     /locus_tag="VVN16_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5571827.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21390..21866)
                     /locus_tag="VVN16_000379"
     CDS             complement(21390..21866)
                     /locus_tag="VVN16_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5571828.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERADLLATYPEHKEMIEHLTRY"
     gene            21873..22400
                     /locus_tag="VVN16_000380"
                     /pseudo
     CDS             21873..22400
                     /locus_tag="VVN16_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(22715..24406)
                     /locus_tag="VVN16_000381"
     CDS             complement(22715..24406)
                     /locus_tag="VVN16_000381"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5571829.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            24542..24988
                     /gene="argR"
                     /locus_tag="VVN16_000382"
     CDS             24542..24988
                     /gene="argR"
                     /locus_tag="VVN16_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5571830.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            25039..27573
                     /gene="mutS"
                     /locus_tag="VVN16_000383"
     CDS             25039..27573
                     /gene="mutS"
                     /locus_tag="VVN16_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5571831.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            27720..29414
                     /gene="patA"
                     /locus_tag="VVN16_000384"
     CDS             27720..29414
                     /gene="patA"
                     /locus_tag="VVN16_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU5571832.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEVIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIESGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(29744..30007)
                     /locus_tag="VVN16_000385"
                     /pseudo
     CDS             complement(29744..30007)
                     /locus_tag="VVN16_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            30201..31967
                     /gene="patB"
                     /locus_tag="VVN16_000386"
     CDS             30201..31967
                     /gene="patB"
                     /locus_tag="VVN16_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU5571833.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSAGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            32106..32795
                     /locus_tag="VVN16_000387"
     CDS             32106..32795
                     /locus_tag="VVN16_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5571834.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVYPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            32799..32978
                     /locus_tag="VVN16_000388"
     CDS             32799..32978
                     /locus_tag="VVN16_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571835.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            32965..34314
                     /locus_tag="VVN16_000389"
     CDS             32965..34314
                     /locus_tag="VVN16_000389"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU5571836.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            34438..35898
                     /locus_tag="VVN16_000390"
     CDS             34438..35898
                     /locus_tag="VVN16_000390"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5571837.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     ESEVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            35912..36346
                     /locus_tag="VVN16_000391"
     CDS             35912..36346
                     /locus_tag="VVN16_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5571838.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        10612 a         6997 c         8019 g        10784 t
ORIGIN      
        1 aaccatacgt acggcaaggc gacgttgacg tggtttgaag agtattagaa tgctttccca
       61 accagaatct ctgcttcgat ggtaatctct gtcagttggc tccagtcaat cttgctctgg
      121 tcaacgtgaa agaggagagg agtcatactg agtagggctt ggagttgttc tgctgtgata
      181 gtcttagtca gagaggcagt ttgactagat aggatggtaa agtgttcttg gaaatgctcc
      241 ttgatatctt ggttagaata ctccttgttt gtcagctggt cctgtactct ttgacggatt
      301 tctttgaggt gattttcagt tgggataacc tttatcaaga taccgtcttt ggataaaacg
      361 cgacgaaatt ctccatagtt ggcaggtgag aagatatcaa gcagaatatc catgttagcg
      421 tcttttatag gaaggcgtgc caagtcgcca acgaaccaat tgactgccca gttgggttca
      481 cttttagcag cgatttggac tgaatctttg gagatgtcaa aggcatagaa agttttttca
      541 gagtgacttt cttgtagttt gcgagaatag aatccttcac cacaaccgat atccaaaatt
      601 gttgtggtag tttttgagct tgcaagcaag tcagatacag catctaagat agcttggtaa
      661 aagccggctt ctaggatttg ttgacggttt tgaaaatttt ccttgtcata gttagcggat
      721 tgcttgattt gaggtgctag attgacatag ccaaatttcg ccaagtcaaa agaatgacgg
      781 ttgcagcact tgaaattagt ctctaacaga gtcagatttt cttgacagat agggcaggca
      841 aaggcagtcg cagaagcaaa acgctgaagt ttgggcttga gatttgtatt catagtttaa
      901 gtgtatcaaa agaggaaaat gaaagcaact ttaggaataa gtagatggga gattcagtag
      961 aaataaaact aatactcttc gaaaatcaaa ttcaaaccac gtcaacgtcg ccttgccgta
     1021 ctcaagtaca gcctgcggct agtttcctag tttgctcttt gattttcatt gagtataatt
     1081 ctccaattta tagaatgaaa ttgcttttat atctaaattg ctatataata atgataagga
     1141 ggaaataagt atgttaaaag aagtattaac cgttgcaaaa gttgcgaaaa aatcttcatt
     1201 atttttgggt ggtgtcgcat ttggtaccct tggtttgaaa atcttagcaa gtaaggaagc
     1261 taaaaaaggt tattctaaag ctttggctaa ggcttacaag ttgaaagacg agctagatgc
     1321 atctgtttct gttgtgaagc aacatggaga cgatgtcttg caagatgcca aatatttgta
     1381 cgagcaagag aaaaaagaag agcaattaga tagccttata ggtgaataat atgtctttta
     1441 aagtgctaca tagaggatac caacatatcc gactatcatc ttctttttca cttaccttgg
     1501 atattcaaga ctatcttcgt tccttggcga gagatgaaaa ggggattgag tctatccagt
     1561 tttacatgga tcaacagcac tttactctac gcataaaaga aggcttttct gtattagata
     1621 atgcagaagc ctttttaaaa agaattgata aagggaaagt ttctgagttg atgactcttc
     1681 ccattcgtag agaagagagt gcttattcta ttgtttcagg tgcagcggtt aagcgtgtac
     1741 tttttcgtag ttttgtgccg tatcctattc gctatatatg gacttgttat caggctttgg
     1801 gttatattag agaagcctat caaacactag cgcgtaagga actaacgatg gaagtcttgg
     1861 actgttcggc tattttattg tctttgttta tgaaccaatc caagacagct agcaatatca
     1921 tgtttatgct tgatttgggg aatcatttag atcaatggtc cttgaaaaaa actgcaacag
     1981 atttagaaca gagtcttctt gcaaaagaga gcgatgtatt cctagtacag ggcgatacgg
     2041 ttgttagtat caagagttcc gatgttcaaa taggagatgt cttgatctta tctcaaggaa
     2101 atgaaattct gtttgatgga caagtagttt caggtttagg tatggtcaac gaaagttcct
     2161 tgacaggaga gagttttcca gttgaaaaaa gagagtctga tttggtttgt gcaaatacag
     2221 tattagaaac tggagagtta cgcattcgtg taacagataa tcagatgaac agccgtattt
     2281 tacagctgat taagttgatg aagaaatctg aagaaaacaa gaaaacgaaa caacgctatt
     2341 tcatcaagat ggcggataaa gtcgtcaaat ataatttctt gggggctggg ctgacttacc
     2401 tattgacagg ttctttttct aaggctattt ctttcctatt ggtcgatttc tcctgcgctt
     2461 tgaaaatctc tactcctgta gcttatttga cagttatcaa ggaagggttg aaccgtgaaa
     2521 tggtgattaa ggatggagat gttctggaga aatatctgga agttgatact ttcttgtttg
     2581 ataagacagg aacaatcaca actagttatc ctatagttga aaaggtgtta ccttttggag
     2641 actatagtga ggaagatatt ctcagaatca gtgcctgtct tgaggaacac atttatcatc
     2701 ctattgctaa tgccatcgtc aagcaagctg agatagaggg aattgaacat gaggaaatgc
     2761 atgggaaact ccaatatatc gcaagcaagg ggatcaaatc tcatatagat ggccaaccag
     2821 ttcttattgg gaattatgtc ttgatgcagg atgagcagat tcatatcagt tcagaacaaa
     2881 atgctttaat tgaagagtac aagagtcact acaatctctt attcttggct tatcaaaatg
     2941 aattgattgg aatgttctgc attcatactc ctttgagaaa agaagcaaaa acagccttgg
     3001 ataaacttaa ggcacagggg aaaaaattga ttctggcaac aggagacacc ttgattagga
     3061 cagaggaatt agtcaaagat ttgccctttg atcaggtcta tacagacttg aaacctgatg
     3121 ggaaatttga gttagtagag aaactgcaga aagcaggtca cactattttg atggttggag
     3181 atggattgaa tgactcagcg gctttaactc tatcagatat cggtgtggtg atgaatgaga
     3241 gtgcagatat ttctaagcag atgagtgata tcttattgtt agataatcgt ttggatttct
     3301 tccaagagtt ggattcgtta tcatcatctt tgcaaacact catcaagaaa aatattcaag
     3361 ataccgttgt cgtaaatagt agtttgattg gctttggctt atttaattgg ctcagtcctt
     3421 caaatctctc tatcttacat aatctaacaa ccttgcgcat tgtcctgcgt agcctgtcta
     3481 ttaaaaatag atagatgaga gttattaaca gcaaaaagga gttacctttc tcgaggttaa
     3541 ctcctttgtt tatagataaa tgttgaatag tttagtaagt caatacaaag tatttcttct
     3601 tcccacgacg gataacagtc agttcattct ctaacttatc agcgtcactc aagacatagt
     3661 caaggtcttg gatgcggtcg ccgtttacgt agatggctcc gttttggacg tcttcacggg
     3721 cttggcgttt tgagttaact ataccagatg agacgagcag ttccacgata ttgttgtgtt
     3781 cgtctgcctg tacttggtag tttggcacac cacgaagtcc ttgtttgagc tctttgacag
     3841 aaaggttttt gatgtttcct gcaaagagtt gctcagtgat gttaagtgct tctttgtagg
     3901 cttcttctcc gtgaacaagt gtaacaactt cacgagccaa gactttttga gccaagcgtt
     3961 cgtgtggcgc tgcttcaaat tgtttacgaa tatcttcaat ctcatcaagt gacaagaaag
     4021 taaagatttt caagaagcga acagcgtcag cgtccatcac gttcatccag aattggtaca
     4081 tttcgtatgg agaagtcttt tcgggattga gccagacggc atttccttct gatttaccaa
     4141 attttttacc agttgcatct gtgattagtg gaacagtgat aacgtgacca gtcttgtccg
     4201 ccttacgacg aagcaattcg gtaccagctg tcatatttcc ccactggtca gaaccaccga
     4261 tttgaagagt gacattatgg tcttggttaa ggacgaagaa gtcgtaccct tgcatgattt
     4321 ggtaagcgaa ctcagtgtaa gaaattcctg tttcgatccg ttttttaaca gattccttac
     4381 tcatcatgta gttgaccgtg aagtattttc caatgtcacg gaggaagtca atgaagctga
     4441 tgctgccaaa ccagtcgtag ttgttgacca tgacagcctt gttttcgcca ttttcaaagt
     4501 caagaaaacg agaaagttgt ccttggatag acttgaccca gccatctact gtgtcttttg
     4561 tttggagact acgttcagca tctttgaagg acggatctcc gatgagacct gtagcaccgc
     4621 caacgagcgc ataaggtttg tgacctgcta gttgcaagcg acgacttgtc aagattgcga
     4681 caaggtggcc taggtgaagg ctgtcagcag ttggatcgta gccagtataa taagaaactt
     4741 gaccttcttc tagggcttta cgcaaagctt cttcatcagt cgtttgaaat atcaaaccac
     4801 gctcttttag ctcatcaaaa atgtgcatgt gtcttttctc ctttttaaaa ttattgtttc
     4861 tacctattgt atcacaaact tgggcaatag cctagcagaa tagggaagaa agtggctatt
     4921 ttattgaaaa tttccctttt gtggtataat agataaagtg aggaaatcca tgcaaaatca
     4981 attaaatgaa ttaaaacgaa aaatgctgga atttttccag caaaaacaaa aaaataaaaa
     5041 atcagctaga cctggcaaga aaggttcaag taccaaaaaa tctaaaacct tagataagtc
     5101 agccattttc ccagctattt tactgagtat aaaagcctta tttaacttac tctttgtact
     5161 cggttttcta ggaggaatgt tgggagttgg gattgctttg gggtacggag tggccttatt
     5221 tgacaaggtt cgggtgcctc agacagaaga attggtgaat caggtcaagg acatctcttc
     5281 tatttcagag attacctatt cggacgggac ggtgattgct tccatagaga gtgatttgtt
     5341 gcgcacttct atctcatctg agcaaatttc ggaaaatctg aagaaggcta tcattgcgac
     5401 agaagatgaa cactttaaag aacataaggg tgtagtaccc aaggcggtga ttcgtgcgac
     5461 cttggggaaa tttgtaggtt tgggttcctc tagtgggggt tcaaccttga cccagcaact
     5521 aattaaacag caggtggttg gggatgcgcc gaccttggct cgtaaggcag cagagattgt
     5581 ggatgctctt gccttggaac gcgccatgaa taaagatgag attttaacga cctatctcaa
     5641 tgtggctccc tttggccgaa ataataaggg acagaatatt gcaggggctc ggcaagcagc
     5701 tgagggaatt ttcggtgtag atgccagtca gttgactgtt cctcaagcag catttttagc
     5761 aggacttcca cagagtccca ttacttactc tccttatgaa aatactgggg agttgaagag
     5821 tgatgaagac ctagaaattg gcttaagacg ggctaaggca gttctttaca gtatgtatcg
     5881 tacaggtgca ttaagcaaag acgagtattc tcagtacaag gattatgacc ttaaacagga
     5941 ctttttacca tcgggcacgg ttacaggaat ttcacgagac tatttatact ttacaacttt
     6001 ggcagaagct caagaacgta tgtatgatta tctagctcag agagacaatg tctccgctaa
     6061 ggagttgaaa aatgaggcaa ctcagaagtt ttatcgagat ttggcagcca aggaaattga
     6121 aaatggtggt tataagatta ctactaccat agatcagaaa attcattctg ccatgcaaag
     6181 tgcggttgct gattatggct atcttttaga cgatggaaca ggtcgtgtag aagtagggaa
     6241 tgtcttgatg gacaaccaaa caggtgctat tctaggcttt gtaggtggtc gtaattatca
     6301 agaaaatcaa aataatcatg cctttgatac caaacgttcg ccagcttcta ctaccaagcc
     6361 cttgctggcc tacggtattg ctattgacca gggcttgatg ggaagtgaaa cgattctatc
     6421 taactatcca acaaactttg ctaatggcaa tccgattatg tatgctaata gcaagggaac
     6481 agggatgatg accttgggag aagctctgaa ctattcatgg aatatccctg cttactggac
     6541 ctatcgtatg ctccgtgaaa atggtgttga tgtcaagggt tatatggaaa agatgggtta
     6601 cgagattcct gagtacggta ttgagagctt gccaatgggt ggtggtattg aagtcacagt
     6661 tgcccagcat accaatggct atcagacctt agctaataat ggagtttatc atcagaagca
     6721 tgtgatttca aagattgaag cagcagatgg tagagtggtg tatgagtatc aggataaacc
     6781 ggttcaagtc tattcaaaag ctactgcgac gattatgcag ggattgctac gagaagttct
     6841 atcttctcgt gtgacaacaa ccttcaagtc taacctgact tctttaaatc ctactctggc
     6901 taatgcagat tggattggga agactggtac aaccaaccaa gacgaaaata tgtggctcat
     6961 gctttcgaca cctagattaa ccctaggtgg ctggattggg catgatgata atcattcatt
     7021 gtcacgtaga gcaggttatt ctaataactc taattacatg gctcatctgg taaatgcgat
     7081 tcagcaagct tccccaagca tttgggggaa cgagcgcttt gctttagatc ctagtgtagt
     7141 gaaatcggaa gtcttgaaat caacaggtca aaaaccaggg aaggtttctg ttgaaggaaa
     7201 agaggtagag gtcacaggtt cgactgttac cagctattgg gctaataagt caggagcgcc
     7261 agcgacaagt tatcgctttg ctattggcgg aagtgatgcg gattatcaga atgcttggtc
     7321 tagtattgtg gggagtctac caactccatc cagctccagc agttcaagta gtagttctag
     7381 cgatagcagt aactcaagta ctacacgacc ttcttcttca agggcgagac gataattctc
     7441 taaatgaagt ggccaatcaa tggattgcca cttttttcct ttcgtgttac aataagggta
     7501 tgaatcagta tcagaaaaag attgttaacg gaaaaattta ttcgctccta tccggcttaa
     7561 tatggggaat ctgtggaatt ttaggagagt acttctttac tcattatcag gtgtcttcgg
     7621 gctggattac ctctatgcgt ttgacactgg cagggagtct tgtacttatt tggtctgcaa
     7681 tacaattaaa atcgcaagtg ctagatattt ggcgagacaa gaaaaattac ctgccctttt
     7741 tagcctatgc tattttgggg attttttcag ttcagtattt tttctatctc tgtgtagaat
     7801 actcaaatgc tacgacagca actattttac agtttattag ccctgtcttt atcctctttt
     7861 acaatcgctt ggtttatcaa aaacgagcgt caaaaagcgc tgttttctat gttttggttg
     7921 ccatgctggg tgtttgcttg atggcgacaa agggagatct ctctcagtta tccatgacgc
     7981 cgctagctct tataacaggt ttgctgagtg ccatgggtgt tatgtttaat gttatcttgc
     8041 cccaaccttt tgctaagcgt tatggttttg ttcctacggt tgggtggggg atgattttgg
     8101 caggtttgtt tagcaatgtc ctctcgccgg tttatcagct ttcctttact cttgatattt
     8161 ggagtatctt gatttgcctc attatcgctt tctttggaac ggcttttgct tttttcattt
     8221 ccatgaaggc tgtgtccttg gtttctcctt tggtggttgc cgttatcagt gccagtgaac
     8281 ctctctcttc tgctctcttg agtgttttgt tcttaggatt agtagtggat tggtccctcc
     8341 ttctagctat agccttgatt attttaccca tgatattttt gtctatagaa gaagcgaaag
     8401 aaagtagata aaaagtcttt tcttaattct aaaagtgtgc tatactagaa tagtcaataa
     8461 aacacgggga gatagttgtg aagagtgttt ttaggttgta tcggtagggg cttgctttta
     8521 ccgacagcac gttttatctc acatccctaa aaaatcatac ctaaaaacag acaaaaaggt
     8581 ttcaaatttg aggccttttt tggtagaata ggtatcatta taacgaacca ggaggcacct
     8641 atgactgcta caaaaatgaa cgctcaagaa attatccaat ttatcgccaa tgctgaaaag
     8701 aaaaccagtg tcaaagtaac ctttgagggg caactcgcaa ctgctgtgcc tagctctgtt
     8761 gtcaaactag gaaatgtcct attcggagac tggaaggacg tggctccgct tcttgaaggt
     8821 ttggtagaaa atcaagatta tgttgtcgag caagatgctc gtaattctgc agttcctttg
     8881 ctagataagc gtgctatcaa cgctcgtatc gagccaggtg ctattatccg tgaccaggtg
     8941 aaaattggtg acaatgctgt tatcatgatg ggagctgtta tcaatatcgg tgctgaaatc
     9001 ggtgctggaa ccatgattga catgggtgcc atccttggtg gccgtgctat cgttggaaaa
     9061 aatagccacg ttggtgcagg tgcagttttg gcaggtgtga ttgagccagc tagtgctgaa
     9121 ccagtccgtg tcggagacaa tgttcttatc ggggctaatg cagtggttat cgaaggagtc
     9181 caaatcggta gtggttcagt tgtcgcagca ggagctattg ttacccaaga tgtcccagaa
     9241 aacgtggtag tagcaggtgt tccagctcgt attatcaaag aaattgatgc ccaaactcaa
     9301 caaaaaacag cgttagagga tgcgcttcgt accttgtaat tgtaaaagta aaaaagaggc
     9361 ggaacccttt ttccagcctc tttctgctat atcggaggac agatagatgt tagatttgat
     9421 tcagactaga cgagatttac accagattcc agagattggc ttggaggagt tcaagactca
     9481 ggcttatttg ctggatgtga ttgagaaatt gactacgggc aaggattttg ttcaaattcg
     9541 tacttggcgg acagggattt tggtctacct gcagggaagt cagccggagc gaaccattgg
     9601 ttggcggaca gatattgatg gcctgcctat cgtcgaacaa acaggtctac cttttgcttc
     9661 tcaacaccaa ggtcgcatgc atgcctgtgg ccatgatttt catatgacca ttgccttagg
     9721 ctgtcttgag cgcgcccttg aggagcaacc taaaaataat ctgctctttc tatttcagcc
     9781 tgctgaagaa aatgaagctg gtggtatgct catgtatgag gctggtgctt ttggagattg
     9841 gttgccagat caattttatg gcctccatgt ccgtccagat ttgaaagtcg gccagattgc
     9901 gactaatact catacactct ttgcaggaac ttgcgaggtg aagatccgtt tcaaaggtaa
     9961 aggtggccac gcggcttttc ctcatgaagc caatgacgcc ttggtggcgg ctagttactt
    10021 tgtgacccaa gtgcagtcag ttgttagccg caatgtaaac ccaatcgagg gagcagtggt
    10081 gaccttcggc gtttttcaag ctggaaccac caacaatgtc atcacagaca cagctttttt
    10141 acatggaact attcgtgcct tgacacagga catgagtctc ttggtgcaaa agagggtaaa
    10201 aacagttgca gaagggattg cagctgcctt tgatatggaa gtcgaagtgg aactcaaaca
    10261 aggtggatac ctacctgttg agaacaatcc agccttggcg cgtgaactga tggacttctt
    10321 tgacgaaaaa gacggaatcg agttgattga tatcgaacct gctatgacag gcgaggactt
    10381 tggttatctc ctttcaaagg ttgatggcgt gatgttttgg ctaggtatcg atagtcccta
    10441 cgctcttcat caccctcaaa tgagtcctaa ggaagaagtc ttagctattg gggtggctgc
    10501 ggtgtctagt ttcctgaaaa agaaggcagc agaatagagg gcttgtctat gaaatcggaa
    10561 ttacgcaagc aagtcttgca tgaaatgaag gctttatctc aagaacaaaa acaggctata
    10621 gaccaagctt taaccgagcg aattttacaa cacccctttt atcaagaagc caaggtcatc
    10681 gcaacctacc tctctttttc tcatgagttt caaacgcggg aactgattga gcaggcgctg
    10741 aaggacggca agaaggtttt aatacccaaa acttatccca aggggcgcat ggactttgta
    10801 gtctatgatc cgcagcagtt ggtaaaaact gcctttgcct tactggagcc acagggagat
    10861 ttggaagtgg tggatgtatc tcagattgat ttgattcatg ttcctggcct ggcttttacg
    10921 acggatggat atcggattgg atatggtgga ggttattatg accgctatct ggaacatttt
    10981 tctggtcata ctttgagtac ggttcatcct tgtcaaattc aggactttat acctgaaaac
    11041 catgatattc ctgttcagga ggtattaatt gatgaaggaa atctttgata gacgttaccc
    11101 tgtgacgagt ttcttcctct tagtgacggc cttggtattt ttactaatgt tggtcactgc
    11161 aggcggaaac tttgacaggg cagatacatt atttcgattt ggagccatgt atgggccagc
    11221 tattcgcctc tttcccgagc aggtttggcg tctcttgtct gccatttttg ttcatattgg
    11281 gtgggaacat ttcattgtta atatgctttc actttattat cttggaaggc aggtagagga
    11341 gattttcggc tctaagcagt tcttttttct ctatctctta tcaggaatga tgggcaatct
    11401 ctttgttttt gtatttagtc ctaaatcctt agcagcaggc gcctctacct ctctttatgg
    11461 gctatttgcc gcgattattg ttcttcgcta tgcaactcgc aatccttata tccaacagct
    11521 agggcaatcc tatctgacac tttttgtggt taacattatt ggaagtgttc tgattccagg
    11581 aatcagccta gcaggccata tcggtggtgc agttggtggc gcatttctag cagttatctt
    11641 tccagttaga ggagaaaaac ggatgtataa taccagccag agattaggag cggtagtctt
    11701 gttcgtagga ctcgccattt tgcttttcta caagggaatg ggaatgtgac gaagttgaaa
    11761 gaaaatttgt gtaatgaaaa aagataaggc attttttgaa ccttatcttt ttattttatt
    11821 ttattccttc tcagtcaatt cttttccaag ttggatgagg taattcttca aatcatcttt
    11881 gacttgtggg tgtttgaggg cgtagtcgat ggatgttttc atgaagccaa acttgtctcc
    11941 gacatcgtaa cgagccccat tgaactcacg agcaaataca cgttgtgttt tattgagggt
    12001 gtcgattgca tctgtcagct gaatttcatt tcctgcacct ggagcttggt tttcgaggat
    12061 ttggaaaatt tcaggcgtga ggaggtagcg tccgataata gcaaggtcgc taggagcgtc
    12121 ctctggagct ggtttttcaa caaaggtttc aacactgtaa agaccatctt ttccttcgcc
    12181 ttgcggagca ataaccccgt aagcagatac ttcgtcatga gggactggca tgacagcgat
    12241 agtagacgcg tgggtacgct cgtagtcatc catgagttgt ttggtaagtg gaacagcctt
    12301 ttcgtctgtg atatccatca agtcatcacc aagcataacg acaaaaggtt catttccgac
    12361 gaaagccttg gcttgcaaaa cagcatctcc gagaccgcgt ggatgagttt ggcggataaa
    12421 atgcagacgc atgtcagttg ttttatcaac tagcttcaaa agatctgttt tccctttttc
    12481 tttgaggtta tattccaatt cgaagtttga atcaaagtgg tcctcaatag aacgttttga
    12541 tttaccagtg acaactagaa tatcttcaat acctgatttg agagcctctt ccacgataaa
    12601 ctggatagtt ggtttgtcta cgattggcaa catttctttg gcaagggcct tggttgctgg
    12661 taaaaatcga gttcctagtc cagcagcagg gatgactgcc tttctaactt ttgatgtcat
    12721 aaaaatcctt tctatagagg gttaagacca ctcattttct gctttaaatt cattgttcat
    12781 gatgtcataa atggcatctt tgatattggt tccgtgataa ataacttggt aaatagcctg
    12841 tgtaatgggc atatagactc caagttcttg ggctagttca taggctgctc gagtcgttga
    12901 aattccttcg attaccatgc ccatattagc ttctatatca gctagggatt ctcctcgtcc
    12961 gagagcatct ccagctctcc agttacgaga gtggatggaa gttcccgtta cgatcaaatc
    13021 tcccacacca gataagccgc tataggtcaa tggactggcc ccgagtgcta cccctaggcg
    13081 ggtgatttct gctaaacctc gagcgatgat ggctgcctta gcattatcac caaatccaag
    13141 accatgtaaa gctccagcac cgacagcaat aatattttta agagcaccag cagtttcaac
    13201 cccgataaca tccgtattgg tataaagtcg gaagtagtga ttactaaata gcttctgaac
    13261 gtattgagct gtttgtaaat ctttagaagc agcagttatt aaagttaggt cacgcacaat
    13321 ggtctcttct gcatgactag gccctgaaac aacgacgata tcactacgga gatgttcagg
    13381 aatttcttct tcaagaatgg ttgataatcg tttatggcta tcaggttcta atccctttga
    13441 tgcgtgcatg atgataacct tatggtccaa ggtttgtgca acttgctggg caacaagtcg
    13501 tgtcactttt gttgggacaa caaacaaaat cgcatccaca tctttcaatg tttctgctaa
    13561 gtcggtgtag gcaatgatat tttcgtctag aacgacatct ttaaagtagt gcttattagt
    13621 atggtgtgta ttaatttcat tgatttgctc gggaagattt ccccaaatac gtacctcgtg
    13681 tccattgtca tttaagactt gtgaaagggc ggttccccaa gaaccaggcc ccaagacggc
    13741 gacggtttgt ttttccatga tttcctcctt gatagagttc tttctcttat tctatcatat
    13801 tctaggggat tttgcacttg cattactggg gtatggtgga aaaatacaca aaaaccgaga
    13861 tgaagggata aactcttagt ctttagttaa agtgagaaga aatctgatag atagtttgat
    13921 tcagtatatg ctataaatag aggtaaacaa ctcaggaggt tcttgtaggt tgcgagagtt
    13981 tggcgaaaaa attaaaagat tacgtttggc taaaaaaatc agtcgttcag aattttgtgg
    14041 tgatgagtct gaattaagta tccgtcaatt aattagaatt gaaaatggag aatccagacc
    14101 aacactaaca aagttaaaat atatcgctga acgtttgggg gttgaagatt acaagttgat
    14161 gccaagttat atagagttgg ataaggaata cctagaattg aagtatttct tgatgaggac
    14221 tcctacatac gaagatgaaa ctatcgccca aaagaaagag agtgttttga taagattttt
    14281 gaagagtatt atgataggct acctgaggaa gaaagattta tcatcccaaa ttattcatat
    14341 ctggcactaa cgaactacac agttcaaaaa ttaccagaaa agctagttga aatactgtcc
    14401 ttctggtgat tttgcttaat ttctgatgtt cgagacataa ttgaggagat ttgtcttgat
    14461 tttttcctaa aaaagggcag actcctccct tggtttgatg ctcgattttt aaaataaggt
    14521 gacttattct gttaggcatg ttttcccctg ccctctagct ctatatggaa cagggagtat
    14581 cagatttatc tggcagattg tatcaaataa ggcttgaaac tgttttgaat aggcgtatag
    14641 acttgagaat ttgagaatat gtcgtctagc agtagagaca tattttccgg caatatgaag
    14701 gaagagacgt cgaaaacgct tgatagtcaa ggcctttact tcatcaccag ctagctgctt
    14761 taaaaagagg tagagattat aagccagaca gcccatcatc atacgtactt cattcttaat
    14821 catggtcgaa ctatctgtct tatccccaaa gaatcctgct ttcctttctt tgataaagtt
    14881 ttcagcattc cctcgttcac gatagaattg aaacagctcc tcactatcta cactgcttaa
    14941 attggtcaaa tgaacttttt tacaaccata aaaaagagca cttaatcgta ctcttctctt
    15001 ttaaggatta gttgaattaa ttcaaatcca ctagttctcc tgtttctagg tagactaggc
    15061 gttcacaaat gttagcaatg taatcagcga agcgttccag atgccctatg atataaaggt
    15121 attgagttcc attgggaatt gaggtttctt ggtctttcat aagtccaatg atttcctttg
    15181 ataaggcata ataatattgg tcaatctgtt catctttttg agcaatacta atagcttttg
    15241 aggcttggtg caaaggaaag gcaaccaata aatcggctag catgctgagg gataatttac
    15301 ccatttggtg taactgttct tcgtcagggg ctagttgatt ttcttttagt tgcaaaacag
    15361 ctttggcaat gcctgccata tggtctccca tacgttcaag gtctgaacaa gaagacatga
    15421 tgctaatcac aaatcgaagg tcagacactt gtggctgctg caaggccaac aaacgggcac
    15481 aggtcaattc gatagcgctt tgaccttggt tgatagcatg atccttattg ataattagct
    15541 ctgccatctc cttgtctttg gaggctaagg ccagtaaggc ttttgaagct gtttcaagga
    15601 caagttgccc tagtcctaaa aaggattgtt ctaattcatg caattctaag tcaaattgat
    15661 ttctcatagg tttttccttc cttatccgaa ccgtcctgaa atatagtctt ctgtgcgctg
    15721 atcttttgga ttggtaaaca cgtcaacggt atctccaaat tcgcaaattt ctcctgttaa
    15781 gaaaaaagca gttttatctg aaatacgtga agcttgttgc atgttatggg taacaatgat
    15841 aatcgtataa tcctttttta gttgctgaat gaggtcttca atttttaaag tggagatagg
    15901 gtctaaggct gaagtcggct catccattaa cagaatatca ggttctactg ctaaagctcg
    15961 cgcaatgcaa aggcgttgct gctgaccgcc agataaggac atggcactct ttttaagatc
    16021 atctttgact tcttcccaaa tggctgcccc ttttaaagat ttctccacta aggcatctaa
    16081 ttgttttttg tctcgaatac catgtgtcct tgggccataa gccacgttat catagataga
    16141 catggcaaag ggattaggct gttgaaaaac catccctaca cgcttacgta gctgattaag
    16201 gttgaatttg ctactataaa tatcttgctc atctaagagg acttggcctt caatatggca
    16261 agaaggaacc aaatcgttca tccggttaag ggtttttaga aaagttgatt tgccacaacc
    16321 agatgggcct atcaaggcag taatctgtct ttctggtaat tgaatcgaaa tattttttaa
    16381 ggcttgaaaa tccccgtaaa ataagtctag gtgtctgact gaaaatgttc ccatactagg
    16441 aagctccttt cacaagtttt cgagataata agcttgatag agtatttatc attaaaacag
    16501 taataatcaa aatcacaccg gtagcatagg cttcattgac atgtagcccc tcacttgaca
    16561 gcatatacat atgcagggct agagaacgac ctgaagacat gagactactt ggcgtattgg
    16621 tagaggtacc taatgtatac atgagggcag ctgtctcacc aacgatacgg ccaatagcta
    16681 gtatcactcc agctaaaata cctggcatgg caactggtag aacaattcta aaaacagtcc
    16741 gtaacttacc tgccccaagt ccataacttg cttgacgcat gctatcacta acagataaaa
    16801 gggcttcttc tgttgagcga ataatgactg gcaacaccat gatgactgag gttaagattc
    16861 ctgataacag agagtattga aaacctaaga agactacaaa gaagagcatg ccaaacagac
    16921 caaaaacaat ggaaggaatc ccagacaagg tatctgaggc caatcgcatg attttaacac
    16981 aaagggaatc tttttttgta tattccacaa gataaaaacc agcaaaaatc cctatgggca
    17041 aggctaaaag aagagcacca aagaccagaa taacggtgga aataatcgct ggcataaggg
    17101 aaatgttctc agaagtataa gtccaagaaa agagggatag acttagatga ggtaaacctt
    17161 tgatgaggat aaaaccaatg attaaaaaga gagagccaaa ggttaaagct gaaaaacaat
    17221 aaacgagaag ttttagcagg tatttactca taagatgatt ttcctttcaa gtaggcaaag
    17281 taggcattaa tcaagagaat aaggaaaaag agaactgctg aggttgcaat aagggcttcc
    17341 ctatgctgac ctgatgcgta agccatttcc agaacaatat tggttgttaa ggttctggtt
    17401 cctgaaaaga gtccacttgg aataatcggc tggttgcctg ccaccaaaat aactgccatg
    17461 gtttcaccta ctgcgcgacc gatgcctaaa ataactgctg ataaaatacc agatctcgca
    17521 gctggcaaga tgacactaaa aatactccgt tcatgactag ctcctagagc caagctacca
    17581 gaataatacg ttttgggaac tgttcggata gcagattctg acaaactgat aatggttggc
    17641 aaaatcatta ttcctaatag taacgaagcg gttaggacac tcatgccatt tcctaaaaag
    17701 cttctaatcc aaggcaccaa taattgtagg ccgaaaaaac cataaacaat agatggaatg
    17761 gccgccatca agttgatagc tgattttaag aagccataga cgggctttgg acaataataa
    17821 accataaaca ccgatgtcaa gatgcctgtt ggcaccccaa tcacaatcgc tcctaaggta
    17881 attaataagg aaccaacgat cattggtaaa ataccatagc ttgccggaat gttcgttggc
    17941 gaccaatcac tgcctaataa aaaacgggca aagccgtagt tagccatgaa aggtaagcca
    18001 ttactaaaaa taaagaaaca gattagcaaa atagctacaa cagctactgt tgcactcatg
    18061 aaaaaaattg ccctaaaaac tgcttctttg aaggcttgtt ttatcacatc ttgtcctttc
    18121 tagtgaagaa agtaagggag atacgacacc tccctacttg ccttctttat cttattgtac
    18181 gatgaaacgt ctgcatctct ttagagattt atggagcaaa cattttattt aatcttgtcc
    18241 caggtggtta atttgccact aaaaacgtct gcaagttcag ccatactgac ttggcttgcc
    18301 ttattgtcat tattgaccac aacagcaata ccgtctaaag caatagcatc atgggtgaga
    18361 ctcttacctt cttcaggagt taattcccta gaaaccatac caatatcagc ggttttctcc
    18421 ttaacagcgg taatacctgc tgaagaccca ttagaggtaa tatcaatcgt aacttctgga
    18481 ttttcttttt tataagcttc tgctaatttt tccattaaag aagatactga agtggaacct
    18541 acaacagaca acttgcctga taagtgttgg cttgtatatt ctgtggtttc agttttagct
    18601 tcaataaatt tattatctgt gaccacttgt tgaccttgtt tggagtggat aaagctgata
    18661 aaatcttgac ctagcttgga aagattagaa gaccaaacaa tgttgaaggg acgttgaaga
    18721 gggtattcac catctaaaac tgtgtctcga ctagccttga caccatcaat ctctaaagcc
    18781 ttgacagatt tcgttaaaga tcccaaggag atgtagccga tagcattagc attcccttga
    18841 actgctgaga gaacaccttc tgtactattt tgaatcacag ctgttttggc agtgttgtca
    18901 atttttttat caccgtcttt tttgagaatc cctgtgattt ctgtgaaggc accccgtgtt
    18961 ccagagccat tttctcgtga aatcacctca atcgttcctg aagctgactg tttggaagca
    19021 gctgactgat tgccacaggc aacaagccca aatcctgata agccaatggc tgcaagagta
    19081 agcatttttt tgaatttcat aataatcacc tttatctcta tgtatttttc ttgtgtaggc
    19141 ttactacatt tatagtctaa caagtctttg taaaggttta tccctgattc atgtaaagat
    19201 tgtgtaaaga atcaaaaaaa gccacttttg aaaaatggct gctcctaaaa atagctttaa
    19261 aaattattag tcctgtgcga aagattggtt aggaagaaaa atcgtgaagc aactgcctct
    19321 gccaagctga cttgtcaccg tgacttggcc acctaataat tgactgagtt ctttgacaat
    19381 ggcaaggcca agaccagtgc caccagtttg tctgcttcga cctttattaa ctcggtaaaa
    19441 acgttcaaaa atacgatcct gctctaattg actaatacca atccctgtat ctgatacaga
    19501 aatcttaatg ccttcgttca ccttttgggt cttgacctca atttttcccc cttgttcagt
    19561 gtaacggatg gcattggata aaagattgag taagatttgg gaaagtaatt gactatctga
    19621 tacgagggtg acatcatctg gcacctgcac ctttagctgt aaatccttct tcttgagctg
    19681 aggttgcaag ctttgagtca aatcctgtac aaattctgcc aaagaaaggg tcgtccattg
    19741 tataggcatt tgttgagcct tagataaggt aagaagatgc tcaacaatat gctcaagacg
    19801 caagctttct ttgtaaataa tgtctagaaa gtcatccttg agcgcttctt cttcagctga
    19861 catcccctta atggtttcag caaagccctt aatcgaagta actggtgtcc tcaattcatg
    19921 ggaggcattt gagacaaagg ctaaatttaa cttttcataa gttctaatcg ttgttaaatc
    19981 atatagcaag acgagcacag cttccacaga ttgggtgggg ttaaaaacgg gaactgctgt
    20041 cacttctaaa atcaagtcac cctcatgaaa cccacttact tcttgtttta accttgtttt
    20101 ttgatcaaag gcttggtgaa ctaaatttcg aatatccatc cgtttgaggt catcaagtga
    20161 acttatgtcg ccgtccacat cgggaaaata atgaggcaga gagcgactgg ataataacat
    20221 ctgaccttga gcggaaacta aaaacgtccc catggttagg tgcgacagaa gaacctccat
    20281 tgtttcggct aggtccttgt attgctgatc ctgttgggag actttggttt ttaggccaga
    20341 cacatactga gccaaagact ttaagtcttc ttgccctttt tctaaaaagt attcactact
    20401 ggtcaagaga ggttggtgca aggtctcaaa agcaacttcc catttccaaa ggcaaaagag
    20461 ccagtagcca ccttgtccca aagaaagggc tagaagaaag agaccgatgc ctttactgat
    20521 ccaagttaat gccatccctg caatcagaat gaggctaaca cttagattga ctagccaaaa
    20581 ttgaaggtag cgtttcatct ataactcctt gaacttataa ccataacccc gaatggttcg
    20641 aataaattga ggggctttag gattgtcttc aattttttcc ctcaacttac caatatgaac
    20701 gtccaccaaa cgtgtttcct gcccaaagtc atacccccag atacgttcca aaagacgctc
    20761 tctagtcagt gtcatgttgg gatgtttcat aagatagagc aagagttcaa attcttttgg
    20821 ggtcaaactc agtaacttat tcgccttgta gacttcatga cgctcagggt atactttcaa
    20881 ggtcccaaat agccaagaat cgtcagcgat attatctgaa tcatctcctt cttgttctcc
    20941 tttagttcgc ctgaggacag ccttgacacg cgccagcaat tctctagggc taaaaggctt
    21001 ggtcaggtag tcatcagccc ctaattccaa ggccaaaacc ttatcaaatt catcactttt
    21061 cgcagaaacc atcataattg gagttttgac gcctttggct ctcagccgct tacaaacttc
    21121 catgccatct aattgtggta acatgatatc aagcaagata aaatcaaagg gttctgtttc
    21181 tgccaaagct aaggccttcc gtccatttgt taccaattga gtagaaaagc cttccttact
    21241 taaatggtag tcaagcaatt tcagaatgtg ttcttcatca tccactaata agacttgttt
    21301 tgtcatctat tatctcctat tggtaacatt ataacacaat tatcagaaat cctaacattg
    21361 ctaaattaga ttaaatttgc ttatcaagac tagtatctgg tcaaatgctc aatcatctcc
    21421 ttgtgctctg gataggtcgc cagtagatct gccctttcaa ataattcaaa atcctcaaat
    21481 tcaaaaccag gagcaacaag acaagaaacc agagcataat ccttatcaac tgttgatccc
    21541 caaatagtac ccttaggaac acagtagtga agttgttgcc ctttggatat gtccaggcct
    21601 aaagtgactg cttcgtagtg accatctgct gtaatcatgt gaacagtaag tggggatcct
    21661 gcatgaaaat accagatttc atctgctgtc aatcggtgaa aatgtgaagg attcgtttct
    21721 tctaataaga aataaatact ggtataaagc gcccttccct taccagcaag gtttatagtg
    21781 tctgaagctt tttttgtttg tctaaaatag ccaccttcaa catggggagc taactctaga
    21841 gttcttatca agtcttcttt atccatcggg gccaatgggt tgaagtaact cttgttcaaa
    21901 gtggttttac gatttcaaga actcctctca gttctgagga cacggtaatg attgatgcga
    21961 cggaagtaaa aatcaatcgc cctaaaaaaa gaattagcga atgattctgg taaaaagaaa
    22021 tttcacgcta tgaaggctca agcgattgtc acaagtcaag ggagaattgt ttctattgcc
    22081 atgatatgaa gttgttcaaa atgagttgca gaaatatcgg acaagctggt aaaatcttgg
    22141 ctgacagtga ttatcaaggg ctcatgaaga tatatcctca agcacaaact ccacgtaaat
    22201 ccagcaaact caagccgcta acagctgaag ataaagccta taaccatgcg ctatctaagg
    22261 aaagaagcaa ggttgagaac atctttgcca aagtaaaaac gtttaaaata ttttcaacaa
    22321 cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct ggtattatca
    22381 atcatgaact aggattctag ttttgcagga agtctattat ttggttaggt gaattagtga
    22441 agtgtttagg caagtgtctc tggttacgac gtcatggcct ctaaatcgat tatatttagg
    22501 ggtcatgact agtgaagcag ttagctagtt cgcatataag cggctagcgt ctaacaatta
    22561 ggaactttag ttccaataac tttaagatta cgacgtttta ggacataaat cgatcatatt
    22621 tatgtcctaa aactagtgaa gcgcctagcc aaagtccgaa taggatttgg cgttagttac
    22681 ttagattgct ttgcaatcaa gtaactttgg cgatttacat cttctctggc gcttctactc
    22741 caagcaagcg aagggcttct ttgagaacga ctgcggttgc gtagctgagg gctagacggc
    22801 tgtcgcgttc tgggctttca tccaagatac gtgtatgtgc atagtatttg ttaaaggatt
    22861 gagccaggct aattgcaaat ttagcaatga tagaaggttc aaagttatct gccgcacggt
    22921 tgataatacg tgggaagtct tgaatgagtt taatgatttc ccagctttca gtatcattca
    22981 agctatagtt gccagctgtt tctggtttga aatcggcttt gcgtaagata gattggatac
    23041 gagcgtaggc atattgaacg taaggtccag tttcaccctc gaaggatacc atagcttcta
    23101 ggtcgaagtc gtatccattt gtacggtcag ttttgaggtc atagaattta atggctccaa
    23161 ccccaacagc atgtgctact tggtctttgt tttctagttc aggattttta gcctcgattt
    23221 ggaccttggc acggctaaca gcctctgcaa cagtaggctc tagcaagatg acattccctt
    23281 tacgagtaga gagcttcttc ccttcttttg taaccagacc aaaaggaacg tgggtaatgt
    23341 cgtcactcca atcgtagccc atctcttgca agacagcttt gagttgttta aagtgggcag
    23401 attgctcttg accaacgacg tagatagatt tagcaaattg gtattcattt ttacggtaga
    23461 gggctgcagc caagtcacgt gtgatataga gagttgcacc atcagacttc ttgatgaggg
    23521 ctggatgttc aattccatat ttctcaagat tcacaacttg ggcaccttct gattcaagaa
    23581 gtagtccttt ttcagaaaga atgtctacaa ctgcatccat cttatcattg tagaaggctt
    23641 ctccgttata gctgtcaaat tcaaccttca attcattgta aaggcggtta aattccacta
    23701 aactttcatc gcggaaccat tgccaaagag cgagagcttc ctcatctcca ttttcaagtt
    23761 tacggaacca ttcgcgcgct tcttcatcca agctagggtc attttcagct tcagcgttga
    23821 tgcggacata gagtttaagg agttcatcga ttggatgagc ttttacagct tcttcgtcgc
    23881 cccatttttt gtaggcaaca atcaacatcc caaattgttt accccagtct cccaaatggt
    23941 tgaccttgac cgtttgataa ccgatttttt ggaaaatatg tgacaagcta tctccgataa
    24001 cagttgaacg caggtggcca atagaaaatg gtttagcgat attcggacta gacatgtcga
    24061 taacaacatt ttcttgtttg ccaatatttt ggtcagcata gtgttctttt tcagtggtaa
    24121 cagcttgcaa tacttgagca gaaatggcag atttatcaag gaaaaagtta acgtaaggtc
    24181 ctgttgcgac aactttttca aaggcttggc tgttcatttt ttcagccagt tcagccgcaa
    24241 tcatttgtgg tgctttacgt tcgacttttg caagagaaaa agcagggaaa gcaatgtctc
    24301 ccatttctga gtttttaggg gtttccagta actttaaaat agcctcttgg tccaggctat
    24361 caatgatgct agataattcg ctagcaatca attcttttgt attcattaag agctcctttt
    24421 tggacttttc tactatttta tcacaatttt aaagaaagaa gaaaaaattt ttgaaatctc
    24481 ctgttttttt ggtataatat ggttataaat atagttataa atatgcacgc aagaggattt
    24541 tatgagaaaa agagatcgtc atcagttaat aaaaaaaatg attactgagg agaaattaag
    24601 tacacaaaaa gaaattcaag atcggttgga ggcgcacaat gtttgtgtga cgcagacaac
    24661 cttgtctcgt gatttgcgcg aaatcggctt gaccaaggtc aagaaaaatg atatggtgta
    24721 ttatgtacta gtaaatgaga cagaaaagat tgatttggtg gaatttttgt ctcatcattt
    24781 agaaggtgtt gcaagagcag agtttacctt ggtgcttcat accaaattgg gagaagcctc
    24841 tgttttggca aatattgtag atgtaaacaa ggatgaatgg attttaggaa cagttgctgg
    24901 tgccaatacc ttattggtta tttgtcgaga tcagcacgtt gccaaactca tggaagatcg
    24961 tttgctagat ttgatgaaag ataagtaagg tcttgggagt tgctctcaag acttattttt
    25021 gaaaaggaga gacagaaaat ggcgatagaa aagctatcac ccggcatgca acagtatgtg
    25081 gatattaaaa agcaatatcc agatgctttt ttgctctttc ggatgggtga tttttatgaa
    25141 ttattttatg aggatgcggt caatgctgcg cagattctgg aaatttcctt aacgagtcgc
    25201 aacaagaatg ccgacaatcc gatccctatg gcgggtgttc cctatcattc tgcccaacag
    25261 tatatcgatg tcttgattga gcagggttat aaggtggcta tcgcagagca gatggaagat
    25321 cctaaacaag cagttggggt tgttaaacga gaggttgttc aggtcattac gccagggaca
    25381 gtggtcgata gcagtaagcc ggacagtcag aataattttt tggtttccat agaccgcgaa
    25441 ggcaatcaat ttggcctagc ttatatggat ttggtgacgg gtgactttta tgtgacaggt
    25501 cttttggatt tcacgctggt ttgtggggaa atccgtaacc tcaaggctcg agaagtggtg
    25561 ttgggttatg acttgtctga ggaagaagaa caaatcctca gccgccagat gaatctggta
    25621 ctctcttatg aaaaagaaag ctttgaagac cttcatttat tggatttgcg attggcaacg
    25681 gtggagcaaa cggcatctag taagctgctc cagtatgttc atcggactca gatgagggaa
    25741 ttgaaccacc tcaaacctgt tatccgctac gaaattaagg atttcttgca gatggattat
    25801 gcgaccaagg ctagtctgga tttggttgag aatgctcgct caggtaagaa acaaggcagt
    25861 cttttctggc ttttggatga aaccaaaacg gctatgggga tgcgtctctt gcgttcttgg
    25921 attcatcgcc ccttgattga taaggaacga atcgtccaac gtcaagaagt agtgcaggtc
    25981 tttctcgacc atttctttga gcgtagtgac ttgacagaca gtctcaaggg tgtttatgac
    26041 attgagcgct tggctagtcg tgtttctttt ggcaaaacca atccaaagga tctcttgcag
    26101 ttggcgacta ccttgtctag tgtgccacgg attcgtgcga ttttagaagg gatggagcaa
    26161 cctactctag cctatctcat cgcacaactg gatgcaatcc ctgagttgga gagtttgatt
    26221 agcgcagcga ttgctcctga agctcctcat gtgattacag atgggggaat tatccggact
    26281 ggatttgatg agactttaga caagtatcgt tgcgttctca gagaagggac tagctggatt
    26341 gctgagattg aggctaagga gcgagaaaac tctggtatca gcacgctcaa gattgactac
    26401 aataaaaagg atggctacta ttttcatgtg accaattcgc aactgggaaa tgtgccagcc
    26461 cactttttcc gcaaggcgac gctgaaaaac tcagaacgct ttggaaccga agaattagcc
    26521 cgtatcgagg gagatatgct tgaggcgcgt gagaagtcag ccaacctcga atacgaaata
    26581 tttatgcgca ttcgtgaaga ggtcggcaag tacatccagc gtttacaagc tctagcccaa
    26641 ggaattgcga cggttgatgt cttacagagt ctggcggttg tggctgaaac ccagcatttg
    26701 attcgacctg agtttggtga cgattcacaa attgatatcc ggaaagggcg ccatgctgtc
    26761 gttgaaaagg ttatgggggc tcagacctat attccaaata cgattcagat ggcagaagat
    26821 accagtattc aactggttac agggccaaac atgagtggga agtctaccta tatgcgtcag
    26881 ttagccatga cggcggttat ggcccagctg ggttcctatg ttcctgctga aagcgcccat
    26941 ttaccgattt ttgatgcgat ttttacccgt atcggagcag cagatgactt ggtttcgggt
    27001 cagtcaacct ttatggtgga gatgatggag gccaataatg ccatttcgca tgcgaccaag
    27061 aactctctca ttctctttga tgaattggga cgtggaactg caacttatga cgggatggct
    27121 cttgctcagt ccatcatcga atatatccat gagcacatcg gagctaagac cctctttgcg
    27181 acccactacc atgagttgac tagtctggag tctagtttac aacacttggt caatgtccac
    27241 gtggcaactt tggagcagga tgggcaggtc accttccttc acaagattga accgggacca
    27301 gctgataaat cctacggtat ccatgttgcc aagattgctg gcttgccagc agacctttta
    27361 gcaagggcgg ataagatttt gactcagcta gagaatcaag gaacagagag tcctcctccc
    27421 atgagacaaa ctagtgctgt cactgaacag atttcactct ttgatagggc agaagagcat
    27481 cctatcctag cagaattagc taaactggat gtgtataata tgacacctat gcaggttatg
    27541 aatgtcttag tagagttaaa acagaaacta taaaaccaag actcactagt taatctagct
    27601 gtatcaagga gacttctttg acaattctcc acttttttgc tagaataaca tcacacaaac
    27661 agaatgaaaa ggagctgacg cattgtcgct cccttttgtc tattttttaa ggagaaagta
    27721 tgctgattca gaaaataaaa acctacaagt ggcaggccct ggcttcgctc ctgatgacag
    27781 gcttgatggt tgctagttca cttctgcaac cgcgttatct gcaggaagtc ttaggcgccc
    27841 tccttactgg gaaatatgaa gttatttata gtatcggggc ttggttgatt ggtgtggccg
    27901 tagtcggtct agttgctggt ggactcaatg ttgtcctcgc agcctatatt gcccaaggag
    27961 tttcatccga ccttcgggag gatgccttcc gtaaaattca aaccttttct tatgctgata
    28021 ttgaacaatt taatgcggga aatctagtcg ttcgaatgac aaatgatatc aaccagattc
    28081 agaacgttgt catgatgacc ttccaaattc ttttcagact tcccctcttg ttcatcggtt
    28141 cgtttatcct agcggttcaa accttacctt ctctgtggtg ggtgattgtt ctcatggtag
    28201 tcttgatttt tggtttgact gctgtcatga tgggaatgat ggggcctcgt tttgctaaat
    28261 ttcaaaccct tcttgagcgc atcaatgcca ttgccaagga aaatttacgt ggcgttcgtg
    28321 tggtcaagtc ctttgtccaa gaaaaagagc aatttgctaa gtttacagag gtctcagacg
    28381 agcttcttgg tcaaaacctt tacattggtt atgccttttc agtagtggaa ccctttatga
    28441 tgttggttgg ttacggggcg gtcttcctct ctatttggct ggtcgcggga atggttcagt
    28501 cggatccgtc tgttgttggt tccatcgctt cttttgttaa ttacctaagc cagattatct
    28561 ttaccattgt tatggttgga tttttgggaa attctgtcag ccgtgccatg atttccatgc
    28621 gtcgtattcg agaaattctt gacgcagagc cagctatgac cttcaaggat atcccagatg
    28681 aagagttggt tggaagtctt agctttgaaa atgtgacctt tacctatcca atggacaagg
    28741 aaccgatgct gaaagatgtg agctttacta ttgaatctgg tcaaatggtt ggtgtagttg
    28801 gagcgactgg tgcaggaaag tcaaccttgg ctcaattgat tccacgtctc tttgatccac
    28861 aggacggggc cattaaaatc ggtggcaagg atattcgaga agtgagtgaa ggaaccctgc
    28921 gtaaaacagt ttccatcgtt ctccaacgtg ccattctttt tagtggaacg attgcagata
    28981 acttgagaca ggggaagggg aatgctactc tatttgaaat ggagcgcgca gccaatattg
    29041 cccaggctag tgaattcatt catcgtatgg agaaaacctt tgaaagtcca gttgaagaac
    29101 ggggaaccaa tttctctggt ggacaaaaac aaaggatgtc gattgcgcgt gggattgtca
    29161 gcaatccacg tattctgatt tttgacgatt cgacctcagc cttggatgcc aaatcagagc
    29221 gcttggtgca agaagctttg aataaggact tgaaggggac gacaaccatt attattgctc
    29281 aaaaaattag ctcggttgtc catgcagaca agatcttggt tctaaatcaa ggacgattga
    29341 ttggtcaagg cacgcatgca gacttggttg ccaacaatgc cgtttaccgt gaaatctatg
    29401 aaacacagaa atgaaagaca aactataaga aaagtcaata gttttatcta aactatttct
    29461 tatttcaatt tgatgatttg gcgatgattt tagagcacgg caaaaagccc ttgaaaaagt
    29521 ccattttttc aaaggtaatc ctgtgttgat ttcagaaatt acatcacttt ttgttcgtca
    29581 aatggcagct cttttttagg atataaaaca gggttcggat aagttttttt gcaaggtgga
    29641 tgatggctac attgtaatgt tttccttatt ctaacttagt cttaagatag gccttaaaag
    29701 caggtgaaaa gcgagcgcat gctttggcag cttgtatgag tgcctaccgc agatgagggg
    29761 aaccccgttt gatcattctt ccagctaaat caatctgacc tgactgataa atagaagaat
    29821 ccagtccagc gaaagcttgt aattgagcag gattatcaaa ggcatgaata ttttgaatct
    29881 cggctaaaat gaccgcccct aaacgattcc caatcccagt aaccgtcgtg atgaccgaat
    29941 ttaactcagc catcaagtca ttgacacatg tttccgcctt gtcaatgagc ctcttgtaat
    30001 gtttgatgtt ttcattacac gagataaaac gtctatgcgt tatcaaactc attaccaatt
    30061 aaaacaaatg tggttagatc ctttcggaaa ttgtcaagcg attggaggaa atgaactaat
    30121 ccacagcggc ttattccaag tataccactt gggctttggc agtagctaac tgcgctaaat
    30181 ataatataag gaggagtaaa atgaagacag ttcaattttt ttggcattat tttaaggtct
    30241 acaagttctc atttgtagtt gtcatcctga tgattgttct ggcgactttt gcccaagccc
    30301 tctttccagt cttttctgga caagcggtga cgcagctagc caatttagtt caagcttatc
    30361 aaaatggcaa tccagaactt gtatggcaaa gcctatcagg aatcatggtc aatcttggcc
    30421 tgctggtttt ggttctattt atctctagtg taatatacat gtgtctcatg acgcgcgtga
    30481 ttgcagaatc gaccaacgag atgcgcaaag gtctctttgg taagcttgct cagttgacgg
    30541 tttctttctt tgaccgtcga caagatggcg atatcctgtc tcattttacc agtgatttgg
    30601 ataatatcct ccaagccttt aacgaaagct tgattcaggt catgagcaat attgttttat
    30661 acattggtct gattcttgtc atgttttcga gaaatgtgac gctggctctc atcaccattg
    30721 ccagcacccc attggctttc cttatgctga ttttcatcgt gaaaatggca cgtaaataca
    30781 ccaacctcca gcagaaagag gtagggaagc tcaacgccta tatggatgag agcatctcag
    30841 gccaaaaagc cgtgattgtg caaggaattc aagaggatat gatggcagga tttcttgaac
    30901 aaaatgagcg cgtgcgcaag gcaaccttta aaggaagaat gttctcagga attcttttcc
    30961 ctgtcatgaa tgggatgagc ctgattaata cagccatcgt catctttgct ggttcggctg
    31021 tacttttgaa tgataagtct attgaaacaa gtacagccct aggtttgatt gttatgtttg
    31081 cacaattttc acagcagtac taccagccta ttatccaagt tgcagcgagt tggggaagcc
    31141 ttcagttggc ctttactgga gctgaacgaa ttcaggaaat gtttgatgca gaggaggaaa
    31201 tccgacctga aaaggctcca accttcacta agttgcaaga aagtgttgaa atcagtcata
    31261 tcgatttttc atacttgcct gataaaccta ttttgaaaga tgtcagcatt tctgccccta
    31321 aaggccagat gacagcagtt gttgggccga caggttcagg aaaaacgact attatgaacc
    31381 tcatcaatcg cttttatgat gttgatgctg gtggtattta ttttgatggt aaagacattc
    31441 gtggctatga cttagatagt cttagaagca aggtgggaat tgtattgcaa gattcggtct
    31501 tgtttagcgg aacgattcga gacaatatcc gatttggtgt gccagatgct agtcaggaaa
    31561 tggttgaggt agcagcaaaa gcaacccaca ttcacgacta tatcgaaagt ttgcctgata
    31621 agtacgatac tcttattgat gatgaccaga gcatcttttc agcagggcag aagcaattga
    31681 tttcaatcgc tcgaaccctg atgacagatc cagaagttct cattctcgat gaagcaactt
    31741 caaacgtaga tacggtgaca gaaagcaaga ttcaacatgc catggaggtg gttgtagcag
    31801 gtagaactag tttcgtcatt gcccaccgct tgaaaaccat tctcaatgca gatcagatta
    31861 ttgtccttaa agatggagaa gtcattgaac gtggtaacca ccatgaactt ttgaagctag
    31921 gtggctttta ttcagaactc tatcacaatc aatttgtttt cgaataagaa agaagttgtc
    31981 ctatgtgggc agctttttct tgtccataaa aaatgtttat cacagcctta aaaaaaacat
    32041 attagacgaa agtcattttg agtgatatga taggactatc gttagcattc gaaaggagag
    32101 gcatcatggc tagaacggtt gtaggagttg ctgcaaatct atgtcccgta gacgcagaag
    32161 gcaaaatcat tcattcatct gtatcttgta gattcgcaga gatcattcgt caagtcggtg
    32221 gtctcccttt agtcattcct gttggtgatg agtcagttgt acgtgattat gtggaaatga
    32281 ttgacaaact cattttgaca ggaggccaaa atgtttatcc tcagttttat ggagagaaaa
    32341 agaccgtcga gagcgatgat tacaatctgg tccgtgacga atttgaattg gcactcttga
    32401 aggaagcgct tcgtcagaat aaaccaatta tggcaatctg tcgcggtgtc caacttgtca
    32461 atgttgcctt tggtggaacc ctcaatcaag aaatcgaagg tcactggcaa ggactacctt
    32521 tcgggacatc tcactctatt gagacagtag aaggaagcgt ggtggctaag ctatttggga
    32581 aagaaagtca ggtcaactca gtccatcgtc aaagcattaa agatttggca cctaatttcc
    32641 gtgtaactgc tattgattca agagaccaaa ctattgaagc gattgagtct atcgacgagc
    32701 accgcattat cggtttacag tggcatccag agtttctggt taatgaagaa gatggcaatt
    32761 tagaattatt tgagtattta ttgaatgaac tgtaacgatt ggaacatctg gtcgttcttt
    32821 cttttatttt ttcaaaattt ttagaatgtg gcattttacg caaacgtttg aattctgata
    32881 aaaattggag aaattcgaca aaaaaacttg aaaaaaacga aggtaagcgt tatgatagaa
    32941 aagaagaaat attggaggaa gaacatgtca catattaaat ttgattattc aaaagtttta
    33001 gacaaatttg ttgcaccaca tgaagtggaa tacatgcaat cacaagtaac agcagcagat
    33061 gaattgatcc gtaaaggaac tggtgctggt agcgacttct tgggatggtt ggaccttcct
    33121 gaaaaatacg accgcgaaga atttgaccgc atcttgaaag ctgctgagca aatcaagtca
    33181 gacagcgatg ttttggttgt gatcggtatt ggtggatctt accttggtgc aaaagcagca
    33241 atcgacttct tgaatcacca ttttgctaac ttgcaaacaa aagaagaacg caaagctcca
    33301 caaatccttt acgcaggaaa ctcaatctca tctacttacc ttgctgactt ggtagagtat
    33361 gttgcagaca aagacttctc agtaaacgta atttctaaat caggtacaac aactgaacca
    33421 gcgattgctt tccgtgtctt taaagaactc ttggttaaga aatacggtca agaagaagct
    33481 aacaaacgta tctatgcaac aactgaccgc caaaagggtg ctgttaaggt tgaagcagac
    33541 gctaacggtt ggggaacatt tgttgttcca gatgatatcg gtggacgctt ctcagtattg
    33601 acagccgttg gtttgcttcc aatcgcagca tcaggagctg acatcaaagc tcttatggaa
    33661 ggtgcgaatg cagctcgcaa agactacact tcagacaaaa tctctgaaaa cgaagcttac
    33721 caatacgcag ctgttcgtaa catcctttat cgtaaaggct atgcaactga gatcttggta
    33781 aactatgagc catcacttca atacttctca gaatggtgga aacaattggc tggtgaatca
    33841 gaaggaaaag accaaaaagg tatctaccca acttcagcca acttctcaac tgacttgcac
    33901 tcacttggtc aatttatcca agaaggaact cgtatcatgt ttgaaacagt tgtccgtgtt
    33961 gacaaacctc gtaaaaacgt gcttattcct actttggaag aagaccttga cggacttggt
    34021 taccttcaag gaaaagacgt tgactttgta aacaaaaaag caactgacgg tgttcttctt
    34081 gcccacacag atggtgatgt accaaacatg tatgtgactc ttccagagca agacgctttc
    34141 actcttggtt acactatcta cttcttcgaa ttggcaattg ccctttcagg ttacttgaat
    34201 gctatcaacc catttgacca accaggtgtt gaagcttata aacgtaacat gtttgccctt
    34261 cttggaaaac caggatttga agaattgagc aaagaactta acgcacgtct ataatagaag
    34321 aaaagagtgg tttgcccact ctttttactc tctttatcca tagaaattgg actcagccaa
    34381 gacttgtgat ataatataga aagcaaaaag gcagacgcct agataatagg agaaactatg
    34441 tcaaaagata tccgcgtacg ttacgcacca agtccaacag gactactaca catcggaaat
    34501 gctcgtacag cattgtttaa ttacttgtat gcgcgccatc atggtggaac atttctcatc
    34561 cgtatcgaag atactgaccg taaacgccat gtcgaggatg gtgaacgttc acaacttgaa
    34621 aaccttcgct ggttaggcat ggattgggat gaaagtccag aatcacatga gaattatcgc
    34681 cagtctgagc gtttggactt gtatcaaaaa tacattgacc aactattagc tgaaggaaaa
    34741 gcctataaat cttacgttac agaagaagag ttggcagctg aacgcgaacg ccaagaagta
    34801 gctggcgaaa caccacgcta catcaatgaa taccttggta tgagtgaaga agaaaaagca
    34861 gcttacatcg cagaacgtga agcagcaggg atcatcccaa ctgttcgttt ggctgtcaat
    34921 gagtcaggta tctacaagtg gcatgatatg gtcaaaggcg atatcgaatt tgaaggtggc
    34981 aatatcggtg gtgactggat tatccaaaag aaagacggtt acccaactta caactttgcc
    35041 gttgccatcg atgaccatga tatgcaaatc tctcatgtta tccgtggaga cgaccatatt
    35101 gctaacacac caaaacagct catggtttat gaagcacttg gttgggaagc tccagagttt
    35161 ggtcacatga ccttgattat caactctgag actggtaaga agttatctaa acgtgatact
    35221 aatacacttc aatttatcga agattaccga aagaaaggtt accttccaga agcagtcttt
    35281 aactttattg ctcttcttgg ttggaaccca ggtggtgaag atgagatttt ctctcgtgaa
    35341 gaactcatta aactttttga tgaaaaccgc ctcagcaagt caccagcagc ctttgatcag
    35401 aaaaaactag actggatgag caatgattat atcaagaatg cagaccttga aaccatcttt
    35461 gaaatggcaa aaccattctt agaggaagca ggccgtttga ctgacaaggc tgaaaaatta
    35521 gttgagctct ataaaccaca aatgaaatca gtagatgaga ttatcccatt gacagatctt
    35581 ttcttctcag atttcccaga attgacagaa gcagagagcg aagtcatgac gggtgaaaca
    35641 gttccaacag ttcttgaagc attcaaagca aaacttgaag cgatgacaga tgataaattt
    35701 gtgacagaaa atatcttccc acaaattaaa gcagttcaaa aagaaacagg tattaaaggg
    35761 aaaaatcttt tcatgcctat tcgtatcgca gtttcaggcg aaatgcatgg gccagaatta
    35821 ccagatacaa ttttcttgct tggacgtgaa aaatcaattc agcatatcga aaacatgcta
    35881 aaagaaatct ctaaataaag aggatacaat aatggacatc tgggaaaaga tgtacgaaga
    35941 agcacagaaa ctatacaatc cacatgaagt atcagacttt gtttatgcca atcatgttgt
    36001 agctgcagta gaagcagaag atggacaaat atttacagga ttctgtatgg agggaacctg
    36061 tggtgttttc catctctgcg cagaacgggc ggccctcttc aatatgtacc aattttcagg
    36121 acaaactaag gttaagaaag tattagcctt tcgagacaaa ccaccttatg gtggaagttc
    36181 agccatgcct tgcggtgctt gtagagaatt ccttttagag cttaatgctg aaaataaaga
    36241 tgcagaattc atgatggact ataatataag aaaaacagtt aaagtcgcag aactaatccc
    36301 ttattggtgg ggagaagaac gtgcttctaa gtttaatgag cgatagaaga gtcagtataa
    36361 ttctggcttt ttttactata agttgaaaaa ataaaaaaaa attagaaaaa gt
//
LOCUS       DAVHIT010000010        35521 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVHIT010000010 DAVHIT010000000
VERSION     DAVHIT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.010"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            30..818
                     /gene="codY"
                     /locus_tag="VVN16_000392"
     CDS             30..818
                     /gene="codY"
                     /locus_tag="VVN16_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU5571839.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            818..1393
                     /locus_tag="VVN16_000393"
     CDS             818..1393
                     /locus_tag="VVN16_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU5571840.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1475..1560
                     /locus_tag="VVN16_000394"
     tRNA            1475..1560
                     /locus_tag="VVN16_000394"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1509..1511,aa:Leu,seq:aag)
     gene            1844..2974
                     /gene="ugpC"
                     /locus_tag="VVN16_000395"
     CDS             1844..2974
                     /gene="ugpC"
                     /locus_tag="VVN16_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5571841.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3121..3267)
                     /locus_tag="VVN16_000396"
     CDS             complement(3121..3267)
                     /locus_tag="VVN16_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571842.1"
                     /translation="MEIKEKTRKLAVSCSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEY"
     gene            3475..4233
                     /locus_tag="VVN16_000397"
     CDS             3475..4233
                     /locus_tag="VVN16_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5571843.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAIDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4320..4832
                     /locus_tag="VVN16_000398"
     CDS             4320..4832
                     /locus_tag="VVN16_000398"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5571844.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5014..5958
                     /locus_tag="VVN16_000399"
     CDS             5014..5958
                     /locus_tag="VVN16_000399"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU5571845.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWINHAVYQETPFDWRKIEDDASAYGYL"
     gene            5967..6644
                     /locus_tag="VVN16_000400"
     CDS             5967..6644
                     /locus_tag="VVN16_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU5571846.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6743..7501
                     /locus_tag="VVN16_000401"
     CDS             6743..7501
                     /locus_tag="VVN16_000401"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5571847.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7539..9044
                     /gene="lytC"
                     /locus_tag="VVN16_000402"
     CDS             7539..9044
                     /gene="lytC"
                     /locus_tag="VVN16_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU5571848.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGVYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9541..10059
                     /locus_tag="VVN16_000403"
     CDS             9541..10059
                     /locus_tag="VVN16_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU5571849.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10189..10695
                     /locus_tag="VVN16_000404"
     CDS             10189..10695
                     /locus_tag="VVN16_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU5571850.1"
                     /translation="MTKKIVAIWAQDEEGVIGKKNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARMEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10695..10865
                     /locus_tag="VVN16_000405"
     CDS             10695..10865
                     /locus_tag="VVN16_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571851.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            10897..12129
                     /gene="clpX"
                     /locus_tag="VVN16_000406"
     CDS             10897..12129
                     /gene="clpX"
                     /locus_tag="VVN16_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5571852.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELKFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12138..12725
                     /locus_tag="VVN16_000407"
     CDS             12138..12725
                     /locus_tag="VVN16_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU5571853.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12736..13116
                     /locus_tag="VVN16_000408"
     CDS             12736..13116
                     /locus_tag="VVN16_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5571854.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13168..14058
                     /gene="rapZ"
                     /locus_tag="VVN16_000409"
     CDS             13168..14058
                     /gene="rapZ"
                     /locus_tag="VVN16_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU5571855.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14055..15032
                     /locus_tag="VVN16_000410"
     CDS             14055..15032
                     /locus_tag="VVN16_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU5571856.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15029..15940
                     /gene="whiA"
                     /locus_tag="VVN16_000411"
     CDS             15029..15940
                     /gene="whiA"
                     /locus_tag="VVN16_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU5571857.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(15974..16942)
                     /locus_tag="VVN16_000412"
     CDS             complement(15974..16942)
                     /locus_tag="VVN16_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5571858.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLNLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17078..17935
                     /locus_tag="VVN16_000413"
     CDS             17078..17935
                     /locus_tag="VVN16_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU5571859.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            17922..18701
                     /locus_tag="VVN16_000414"
     CDS             17922..18701
                     /locus_tag="VVN16_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU5571860.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18725..20077
                     /locus_tag="VVN16_000415"
     CDS             18725..20077
                     /locus_tag="VVN16_000415"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5571861.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20151..20525
                     /locus_tag="VVN16_000416"
     CDS             20151..20525
                     /locus_tag="VVN16_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU5571862.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20527..21375
                     /locus_tag="VVN16_000417"
     CDS             20527..21375
                     /locus_tag="VVN16_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5571863.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21652..22419
                     /locus_tag="VVN16_000418"
     CDS             21652..22419
                     /locus_tag="VVN16_000418"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU5571864.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDRKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22431..23615
                     /locus_tag="VVN16_000419"
     CDS             22431..23615
                     /locus_tag="VVN16_000419"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU5571865.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSLLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23612..25483
                     /locus_tag="VVN16_000420"
     CDS             23612..25483
                     /locus_tag="VVN16_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5571866.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25626..26810)
                     /gene="mntE"
                     /locus_tag="VVN16_000421"
     CDS             complement(25626..26810)
                     /gene="mntE"
                     /locus_tag="VVN16_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU5571867.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27114..29810
                     /locus_tag="VVN16_000422"
     CDS             27114..29810
                     /locus_tag="VVN16_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU5571868.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29823..30614
                     /gene="yghU"
                     /locus_tag="VVN16_000423"
     CDS             29823..30614
                     /gene="yghU"
                     /locus_tag="VVN16_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU5571869.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30730..31140
                     /locus_tag="VVN16_000424"
     CDS             30730..31140
                     /locus_tag="VVN16_000424"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5571870.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31152..32747
                     /locus_tag="VVN16_000425"
     CDS             31152..32747
                     /locus_tag="VVN16_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571871.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            32813..32977
                     /locus_tag="VVN16_000426"
     CDS             32813..32977
                     /locus_tag="VVN16_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU5571872.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            33056..33784
                     /gene="yaaA"
                     /locus_tag="VVN16_000427"
     CDS             33056..33784
                     /gene="yaaA"
                     /locus_tag="VVN16_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU5571873.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(33835..35319)
                     /locus_tag="VVN16_000428"
     CDS             complement(33835..35319)
                     /locus_tag="VVN16_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5571874.1"
                     /translation="MRNLLSTKVQRQLRLMENLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIINLVNHLNTLSNEIHLSNTL"
BASE COUNT        10565 a         6522 c         8117 g        10317 t
ORIGIN      
        1 aatgatagcg attaaattcg aggtgaaaaa tggcacattt attagaaaaa actagaaaaa
       61 ttacatcaat tttgaaacgc tcagaggagc agttgcagga tgagcttcct tacaacgcta
      121 ttacgcgtca gttagcggat attattcatt gcaatgcctg cattatcaat agtaagggac
      181 gtctgcttgg ctattttatg cgttataaaa caaatacaga tcgcgtagag caattcttcc
      241 aaactaagat tttcccagat gactacgttc aaggggctaa tatgatttac gaaacagaag
      301 caaacttacc tgttgagcat gatatgagta ttttccctgt tgagagtaga gatgattttc
      361 cagatggctt gacgactatt gcaccgattc atgtatcggg gattcgcctt ggttctttga
      421 ttatttggcg taatgataaa aaattcgaag atgaggactt ggttcttgtt gagattgcca
      481 gtaccgttgt tgggattcag cttcttaact tccaacgtga agaagatgag aaaaatattc
      541 gtcgtcgtac tgctgtcacc atggcggtca ataccctttc ttactccgaa ctccgtgctg
      601 tttcagcaat tttaggggaa ttaaatggaa atgaagggaa gttgactgcg tcagtgattg
      661 cagatcgtat cggaatcact cgctctgtga ttgtcaatgc tcttcgtaaa cttgagtctg
      721 cggggattat tgaaagtcgc tcacttggaa tgaagggaac ctatcttaag gtcttgattt
      781 cagatatttt tgaagaagtg aagaaaagag attactaatg acaaaggctt taatttcgat
      841 tgattataca gaagattttg ttgctgatag tgggaaacta acagcaggtg ctccagctca
      901 ggcgatttcg gatgccatca gcaaggtaac tcgattagct tttgaacgag gagattatat
      961 cttctttact attgatgctc atgaagaaaa cgattgtttc catccggaaa gtaagttatt
     1021 tcctcctcat aatctgattg ggacgagtgg acggaattta tatggagatt tggggatctt
     1081 ttatcaagag catggttcag acagtcgtgt cttttggatg gataaacgcc attactcagc
     1141 tttttcaggg actgacctag atattcgttt gagagagcgt agagtgtcta ccgttatctt
     1201 aacaggtgtc ttgacggata tctgtgtcct acatacagct atagatgcct ataatctagg
     1261 atatgacatc gagattgtta aaccagctgt tgcttccatc tggcctgaaa atcatcaatt
     1321 tgccctaggt catttcaaaa atacactcgg agctaagtta gtagatgaaa atctaaatga
     1381 actttctgag taacctggtt ggtgaaaatg aaaaaatttg aaaaaaagtg ttgacaagca
     1441 tcccaaaagt tgatatacta gtaaagtaat cgacgcgggg atggcggaat tggcagacgc
     1501 gcaggactaa ggatcctgtg accgctttag gtcgtgaggg ttcaagtccc tctctccgca
     1561 taggaaaaga gctagctaaa gctagctctt ttgtatttct ctaaaaaatt agaatccagt
     1621 caaatgtagt ggagaatcag cgaaaatatg ggaaaactga tccttgtttc tgcttttgtc
     1681 tatagaagaa taataaagat tatcttcttc aaattctccg atattctcta aagttttgtg
     1741 caagttgcac agaacttttt tatttttttg gtcatcttgc catagaaata taaagcgttt
     1801 tcatctataa tataattatc aaaagacaaa aggagttcac ctcatggtag aattgaatct
     1861 taaaaatatt tacaaaaaat atccaaacag cgaacactat tcagttgaag atttcaactt
     1921 gaacatcaaa gataaagaat ttatcgtttt cgtaggacct tcaggatgtg gtaaatcaac
     1981 tacactccgt atgattgctg gtcttgaaga cattacagaa ggtactgcat ctatcgatgg
     2041 cgtggttgtc aacgacgtag ctccaaaaga ccgtgatatc gccatggtat tccaaaacta
     2101 cgctctttac ccacacatga ctgtttatga caacatggct ttcggtttga aattgcgtaa
     2161 atacagcaaa gaagacatta acaaacgtgt tcaagaagca gctgaaatac ttggattgaa
     2221 agaattcttg gaacgtaaac cagctgacct ttcaggtggt caacgtcaac gtgttgccat
     2281 ggggcgtgcg attgtccgtg atgcgaaagt attcttgatg gacgaacctt tgtcaaactt
     2341 ggatgccaaa cttcgtgtat caatgcgtgc tgaaatcgct aaaattcacc gtcgtatcgg
     2401 agctacaacg atctatgtaa ctcacgacca aacagaagcg atgacacttg cagaccgtat
     2461 cgttattatg tcagctacta agaaccctgc tggtacaggt actatcggac gtgtagaaca
     2521 aatcggtact cctcaagaag tttacaaaaa tccagttaac aaattcgttg caggattcat
     2581 cggaagccca gctatgaact tcatcaacgt gaaattggtt ggtagcgaaa ttgtttctga
     2641 cggtttccgt ttgaaagtgc cagaaggagc attgaaagtt cttcgtgaaa aaggctacga
     2701 aggaaaagaa ttgatctttg gtatccgtcc agaagacgtg aatgcagaac ctgctttcct
     2761 tgaaacattc ccagactgtg ttgtaaaagc gactatctct gtatcagaac tgcttggttc
     2821 agaatctcac ctttactgcc aagttggtaa agacgagttt gttgcaaaag ttgatgctcg
     2881 tgactacttg caaacaggtg caacagttga gcttggattt gacttgaaca aagcacactt
     2941 cttcgatgta gaaactgaaa aaacaatcta ctaaaataaa taaaattcaa agcactacaa
     3001 gaaaagatat ctctttatca attgtagtgg agagatatca gttaatctag ggagagaaac
     3061 aaaatgcttc tctccttttt gctagggaag tcatattatg catctatatt gtgatgctct
     3121 ttaatactct tcgaaaatct cttcaaacca cgtcaacgtc gccttgccgt acgtatgatt
     3181 actgacttcg tcagttttat ctgcaacctc aaagctgtac tttgagcagc ttacggctag
     3241 ttttctagtt ttctctttga tttccattga gtattatttg tgggtaccat ctacaagtga
     3301 agctatatgc gtaaactacg tgagcaattg aattcgaact agagaggtaa taataaattt
     3361 atgctatagt tatggtgact tgtatgcttt tgattctagt ttatcaaata atagattaga
     3421 attgtcagat aatatcattt tgtgttataa tgaagaaaaa acagaggtgt tcaaatgtca
     3481 gaagcaggtc ataagttttt agcaaaattg gggaaaaaac gcttacgtcc aggtggaaag
     3541 cgtgccacag attggttaat tgcagaagga ggattttcaa aagaaaagag aatactagag
     3601 gttgcgtgta ataggggaac tacagcaatt gagttggcac agcgttttgg ttgcaagata
     3661 actgctattg atatggatgc tcaagcttta gaagtggcta aaaaatctgc tggaacggca
     3721 ggtgttgctc atttaatcag ttttgaaaga gcaaatgcaa tgaaacttcc ttatcaagat
     3781 gctagttttg atattgttat aaatgaagct atgctgacta tgcaagccga tcaagctaag
     3841 aaaaaatgtg taatggaata tctaagggta ttaaaacctg gaggtcttct cttgacacat
     3901 gatgtgcttc ttaaggaagc taaagagtct atcagacagg aattatcaca agcaattcat
     3961 gtaaatgtag gtcctttaac tcaagatggt tgggaacagg tgatgataga atcaggttat
     4021 tgtgatgtga aagcattgac tggtgaaatg acattaatga aattatcggg tatgatttat
     4081 gacgaaggtt tgctaggaac tttgaaaatt tgtgtaaatg cttgtaaaaa ggagaataga
     4141 aagcagtttt taactatgta taaaatgttt gctaagaata aacagaaatt gggctttatt
     4201 gcgatggcta gttataaatc gtcaaaacgt tagataatta ttgaagttaa cttttccttt
     4261 tttctttctt aaaaaatatg ctataataga gagtaaaaaa ctttgaaaga aagaaaaaga
     4321 tgaatttaaa agattacatt gcaacaattg aaaattatcc aaaggaaggc attaccttcc
     4381 gtgatattag tcctttgatg gctgatggaa atgcttatag ctacgctgtt cgtgaaatcg
     4441 ttcagtatgc tactgacaag aaagtcgaca tgatcgtggg acctgaagct cgtggattta
     4501 tcgtgggttg tccagttgcc tttgagttgg gaattggttt tgcgcctgtt cgtaagccag
     4561 gtaaattgcc acgcgaagtt atttctgctg actatgaaaa agagtacggt gtcgatacct
     4621 tgactatgca cgcggatgcc attaagccag gtcaacgtgt tcttattgta gatgaccttt
     4681 tggcgacagg tggaactgtt aaggcaacta tcgagatgat tgaaaaactt ggtggtgtta
     4741 tggcaggttg tgccttcctt gttgaattgg atgaattgaa cggccgtgaa aaaattggtg
     4801 actacgacta caaagttctt atgcattatt aatgaaaaca gtccctaggg ctgttttctc
     4861 tacactagga tataaaaata gactataact agttagagaa aaactataat tgaaaactat
     4921 atcttcttac agtataataa aaggactaag tgtttgagat ttgtcttcaa acatatgcaa
     4981 ttattcctga aagagtacag ttaggagagg gttatgccga ttcgaattga taaaaaattg
     5041 ccagctgttg agattttacg gacagagaat atctttgtca tggatgatca acgtgctgcc
     5101 caccaagata tccgtccttt gaagatttta attttaaatc tcatgccaca gaaaatggtc
     5161 acagagaccc agttgttgcg ccacttggct aatacacccc tacaactgga tattgatttt
     5221 ctctatatgg agagccaccg ttctaaaaca actcgttcag agcacatgga gaccttctat
     5281 aaaacttttc ctgaagtcaa ggatgagtat tttgatggga tgatcatcac gggtgctcca
     5341 gttgagcatt taccatttga ggaagtggac tattgggagg aatttagaca gatgcttgag
     5401 tggtctaaga ctcatgtcta ttcgaccctt catatctgtt ggggggctca ggctgggctt
     5461 tatctgcgct atggtgtaga aaaataccag atggacagta agctatcagg tatttatcct
     5521 caggacaccc taaaagaggg tcaccttcta tttagaggct ttgatgatag ctatgtatcc
     5581 cctcattcac ggcacacgga gatttctaag gaagaggtct taaacaagac caatctcgag
     5641 attttatcag aaggacctca ggttggggtt tctattttgg ccagtcgtga tttacgagaa
     5701 atttatagtt ttggtcattt ggagtatgac cgtgatacct tggcaaaaga gtattttcga
     5761 gatcgtgatg caggtttcga cccacatatt ccagaaaatt actttaaaga tgatgatgtt
     5821 aatcaggtac cttgtctttg ttggtcttca tctgcagccc tctttttcag taattggata
     5881 aaccatgcgg tctatcagga gacgcctttt gattggagaa agatagaaga tgatgcatct
     5941 gcatatgggt atttataaga ggaattatga catatttaga cgcttttaaa tcaggtatct
     6001 tggttttacc gagtgccctg ctcttgcatt ttaaggaact ctttccttct agcgacgatt
     6061 ttctggtttg gcaatttttc tatttgcaaa atacgacagg cttagaagaa atgtcgccaa
     6121 gccagattgc tgaaaggatt ggcaaggaaa tttcggatgt caaccagtcc atttctaatc
     6181 tgacggaaag gggactgctc cagtatcgta ctatcgaatt aaatggcgaa attgaattgc
     6241 tctttgatgc tagtttggcc ttggaacgtt tggatgacct gtttggagca gttcattcaa
     6301 gttcagacca gctgacacct caaaaccagc tcaaggattt ggtggaaacc ttccagcagg
     6361 agttgggacg attgttgacg ccttttgaga ttgaggattt gaccaagaca ctaaaggaag
     6421 atggaaccag tgctgacttg attaaggagg ctcttcgtga agctgttttg aatggaaaac
     6481 caaactggaa gtacattcag gcgattttga gaaactggcg ccatgaagga atcaagagtg
     6541 tggctcaaat tgaggccaag agagcagaaa gagaagcaag caatcctcag ttgacacagg
     6601 tatctgcaga tttcagaaat gccatggatc tctggaagga ttaatccatg caagcaggct
     6661 tgaaatccga gtaagatttg caagctgtgt ataattgtga tagaataaaa tagaaaataa
     6721 attgaaaaaa gaggtatgtg aaatgtcacg taaaccattt atcgctggta actggaaaat
     6781 gaacaaaaat ccagaagaag ctaaagcatt cgttgaagca gttgcatcaa aacttccttc
     6841 atcagatctt gttgaagcag gtatcgctgc tccagctctt gatttgacaa ctgttcttgc
     6901 tgttgcaaaa ggctcaaacc ttaaagttgc tgctcaaaac tgctactttg aaaatgcagg
     6961 tgctttcact ggtgaaacta gcccacaagt tttgaaagaa atcggtactg actacgttgt
     7021 tatcggtcac tcagaacgcc gtgactactt ccatgaaact gatgaagata tcaacaaaaa
     7081 agcaaaagca atctttgcga acggtatgct tccaatcatc tgttgtggtg aatcacttga
     7141 aacttacgaa gctggtaaag ctgctgaatt cgtaggtgct caagtatctg ctgcattggc
     7201 tggattgact gctgaacaag ttgctgcctc agttatcgct tatgagccaa tctgggctat
     7261 cggtactggt aaatcagctt cacaagacga tgcacaaaaa atgtgtaaag ttgttcgtga
     7321 cgttgtagct gctgactttg gtcaagaagt cgcagacaaa gttcgtgttc aatacggtgg
     7381 ttctgttaaa cctgaaaatg ttgcttcata catggcttgc ccagacgttg acggtgccct
     7441 tgtaggtggt gcgtcacttg aagctgaaag cttcttggct ttgcttgact ttgtaaaata
     7501 atcagtaagt agcaaaagct aggtggaaca gcattcagat gtctgttaca ttttttatag
     7561 gagaggaaag attgaaaata aaaattggat tagcaagtat ctgtttacta ggcttggcaa
     7621 ctagtcatgt cgctgcaaat gaaactgaag tagcaaaaac ttcgcaggat acaacgacag
     7681 cttcaagtag ttcagagcaa aatcagtctt ctaataaaac gcaaacgagc gcagaagtac
     7741 agactaatgc tgctgcccac tgggatgggg attattatgt aaaggatgat ggttctaaag
     7801 ctcaaagtga atggattttt gacaactact ataaggcttg gttttatatt aattcagatg
     7861 gtcgttactc gcagaatgaa tggcatggaa attactacct gaaatcaggt ggatatatgg
     7921 cccaaaacga gtggatctat gacagtaatt acaagagttg gttttatctc aagtcagatg
     7981 gggtttatgc tcatcaagaa tggcaattga ttggaaataa gtggtactac ttcaagaagt
     8041 ggggttacat ggctaaaagc caatggcaag gaagttattt cttgaatggt caaggagcta
     8101 tgatgcaaaa tgaatggctc tatgatccag cctattctgc ttatttttat ctaaaatccg
     8161 atggaactta tgctaaccaa gagtggcaaa aagtgggcgg caaatggtac tatttcaaga
     8221 agtggggcta tatggctcgg aatgagtggc aaggcaacta ctatttgact ggaagtggtg
     8281 ctatggcgac tgacgaagtg attatggatg gtgctcgcta tatctttgcg gcctctggtg
     8341 agctcaaaga aaagaaagat ttgaatgtcg gctgggttca cagagatggt aaacgctatt
     8401 tctttaataa tagagaagaa caagtgggaa ccgaacatgc taagaaaatc attgatatta
     8461 gtgagcacaa tggtcgtatc aatgattgga aaaaggttat tgatgagaac gaggtggatg
     8521 gtgtcattgt tcgtctaggt tatagcggta aagaagacaa ggaattggcg cataacatta
     8581 aggagttaaa ccgtctggga attccttatg gtgtctatct ctatacctat gctgaaaatg
     8641 agaccgatgc tgagaatgac gctaaacaga ccattgaact tataaagaaa tacaatatga
     8701 acctgtctta ccctatctat tatgatgttg agaactggga atatgtaaat aagagcaaga
     8761 gagctccaag tgatacagat acttgggtta aaatcatcaa caagtacatg gacacgatga
     8821 agcaggcggg ttatcaaaat gtgtatgtct atagctatcg tagtttatta cagacgcgtt
     8881 taaaacaccc agatatttta aaacatgtaa actgggtagc ggcctatacg aatgctttag
     8941 aatgggaaaa cccttattat tcaggagaaa aaggttggca atatacctct tctgaataca
     9001 tgaaaggaat ccaagggcgc gtagatgtca gcgtttggta ttaagcaatg atttgaaaga
     9061 gggatgtgat agtagcaccc tctttttctt tgttttatga tagttcatcc tcgagtaaat
     9121 tcaagatctt gctcggaaat gaagcttata tagtagattg aatatagaca aataccttgt
     9181 gattggtaaa acattttaga aattcattta cctttcctaa tcgacttggt ttcatcttat
     9241 ttcaatctat tatagtattg gggaatttct tcaaaccaca tcagcttggt cagttctacc
     9301 tgcgacctca aaacttgtgc tttggtcaag ctgggtttag tttcctagtt tgctgatgga
     9361 tttccattga ctataagcat ccaaacctct tttttgtctt ctaaagaatt cttaaattat
     9421 cagtctattg caacttttct catataagtt ctttgtcttg ctattggttt tccttagtag
     9481 tatactaagg tagtaatcat taagaagtgg ttacaaaaaa taatgaatga ggtaaagaaa
     9541 atggtagaat tgaaaaaaga agcagtaaaa gacgtaacat cattgacaaa agcagcgcca
     9601 gtagcattgg caaaaacaaa ggaagtcttg aaccaagctg ttgctgattt gtatgtagct
     9661 cacgttgctt tgcaccaagt gcactggtat atgcatggtc gtggtttcct tgtatggcat
     9721 ccaaaaatgg atgagtacat ggaagctctt gacggtcaat tggatgaaat cagcgaacgc
     9781 ttgattacac tcggtggaag cccattctct acattgacag agttccttca aaatagtgaa
     9841 atcgaagaag aagctggtga ataccgtaat gttgaagaaa gcttggaacg tgttcttgtt
     9901 atctaccgtt acttgtcaga acttttccaa aaaggtttgg atgtcactga tgaagaaggt
     9961 gacgatgtga caaacggtat ctttgcaggc gctaaaactg aaacagataa aacaatttgg
    10021 atgcttgcag ccgaacttgg acaagcacct ggtttgtaag aaataaggaa acatagataa
    10081 atctgtagga aatttcttac agattttttc tattttgtaa gctattccaa accagtcttt
    10141 ttttgagttt tttgataaaa tagtactatc agtgaaaagg atggaagcat gactaagaaa
    10201 atcgtagcta tttgggccca ggatgaagag ggtgtgattg gtaagaaaaa tcgtctgcct
    10261 tggcatttgc cagcagaatt gcagcacttt aaagaaacaa ctctgaatca tgctatcttg
    10321 atggggcgtg tgacctttga tgggatgggg cgtcgcttgc ttccaaaacg ggaaaccttg
    10381 attttgacgc gtaatccgga agaaaagata gatggggttg ctacttttca ggacgtccag
    10441 tctgttcttg actggtatca ggctcaagaa aagaatctct acattatcgg tgggaagcaa
    10501 atttttcagg cttttgaacc ttaccttgat gaagtgattg tcactcacat tcatgctcgg
    10561 atggaaggag atacctattt ccctgaagaa cttgacttgt ctctttttga gacagtttca
    10621 agcaaatttt acgccaaaga tgagaagaat ccttatgatt ttaccatcca ataccgcaag
    10681 agaaaggaag tctaatggaa cggagtttat ttggtctatt cacggctttt ctgtgtttca
    10741 tctgctttct ggcaggagca caagcttttc gtaaaaagcg ttacgggctt tctatcctcc
    10801 tttggttaaa tgcttttacc aatctggtca atagtattca tgctttttac atgaccttat
    10861 tttagataga atgacagtat agaatggaag gaaatcatgt ctacaaatag aaaaaatgat
    10921 atgatggttt attgctcatt ttgtggcaaa aaccaagaag aagtacaaaa aataattgct
    10981 ggcaacaatg cttttatttg taatgaatgc gtggagttag ctcaggaaat cattcgagaa
    11041 gaattggttg aggaagtctt ggcagacttg tctgaggtgc caaaaccaat tgaactcctc
    11101 catatcttga accactatgt aattggtcaa gatcgtgcca agcgtgcctt ggcagtggcg
    11161 gtttataacc actacaaacg catcaatttc cacgatacac gcgaagagtc agaagatgtg
    11221 gatttgcaga agtcaaacat tttgatgatt ggcccaactg gttcagggaa aactttcctt
    11281 gcccagacct tggctaagag cttgaatgta ccttttgcta ttgcggatgc gacagctctg
    11341 acggaggctg gttatgtggg tgaggatgtg gaaaatatcc ttctcaaact cttgcaggtt
    11401 gctgacttta acatcgaacg tgcagagcgt ggcattatct atgtggatga aattgacaag
    11461 attgccaaga agagtgagaa tgtgtctatc acacgtgatg tttctggtga aggggtgcaa
    11521 caagcccttc tcaagattat cgagggaact gttgctagcg tgccgcctca aggtggacgc
    11581 aaacatccac aacaagagat gattcaagtg gatacaaaaa atatcctctt catcgtgggt
    11641 ggtgcttttg atggtattga agaaattgtc aaacaacgtc tgggtgaaaa agtcatcgga
    11701 tttggtcaaa acaataaggc gattgacgaa aacagctcat acatgcaaga aatcatcgct
    11761 gaagacattc aaaaatttgg tattatccct gagttgattg gacgcttgcc tgtttttgcg
    11821 gctcttgagc aattgaccgt tgatgacttg gttcgcatct tgaaagagcc aagaaatgcc
    11881 ttggtgaaac aataccaaac cttgctttct tatgatgatg ttgagttgaa atttgacgac
    11941 gaagcccttc aagagattgc taataaagca atcgaacgga agacaggggc gcgtggactt
    12001 cgctccatta tcgaagaaac catgctagat gttatgtttg aggtgccgag tcaggaaaat
    12061 gtgaaattgg ttcgcatcac taaagaaact gtcgatggaa cggataaacc gatcctagaa
    12121 acagcctaga ggtgactatg gaacttaata cacacaatgc tgaaatcttg ctcagtgcag
    12181 ctaataagtc ccactatccg caggatgaac tgccagagat tgccctagca gggcgttcaa
    12241 atgttggtaa atccagcttt atcaacacta tgttgaaccg taagaatctc gcccgtacat
    12301 caggaaaacc tggtaaaacc cagctcctga acttttttaa cattgatgac aagatgcgct
    12361 ttgtggatgt gcctggttat ggctatgctc gtgtttctaa aaaggaacgt gaaaagtggg
    12421 ggtgcatgat tgaggagtac ttaacgactc gggaaaatct ccgtgcggtt gtcagtctag
    12481 ttgaccttcg tcatgacccg tcagcagatg atgtgcagat gtacgaattt ctcaagtatt
    12541 atgagattcc agtcatcatt gtggcgacca aggcggacaa gattcctcgt ggtaaatgga
    12601 acaagcatga atcagcaatc aaaaagaaat taaactttga cccgagtgac gatttcatcc
    12661 tcttttcatc tgtcagcaag gcagggatgg atgaggcttg ggatgcaatc ttagaaaaat
    12721 tgtgaggaaa agaaaatggc aaaaacaatt catacagata aggccccaaa ggctatcgga
    12781 ccctatgttc aaggaaaaat cgttggcaac cttttgtttg ctagcggtca agttccccta
    12841 tcccctgaaa ctggggaaat tgtaggagag aatatccaag aacagacaga gcaagtcttg
    12901 aaaaacatcg gtgctatttt ggcagaagca ggaacagact ttgaccatgt tgtcaaaaca
    12961 acttgcttct tgagcgatat gaacgacttt gttcctttta atgaggttta ccaaacggcc
    13021 ttcaaagagg aattcccagc tcgttcagct gtggaggtag ctcgtcttcc tcgtgatgta
    13081 aaagtcgaaa ttgaagtcat cgcagagatt ggataagcta gttgaagttt ggtgttgcca
    13141 aacttctttt gatataagga gaaaaagatg acaaagaaac aacttcactt ggtgattgtg
    13201 acagggatga gtggcgcagg gaaaactgta gccattcagt ccttcgagga tctaggttat
    13261 ttcaccattg ataatatgcc gccagctctc ttgcctaagt ttttgcagct ggttgaaatt
    13321 aaggaagata atcctaagtt ggctttggta gtggatatgc gtagccgttc tttcttttca
    13381 gagattcaag ctgttttgga tgagttggaa aatcaagatg gtttggattt caaaatcctc
    13441 tttttggatg cggctgataa ggaattggtc gctcgttaca aggaaaccag acggagtcac
    13501 ccactagcag cagacggtcg tattttagat ggaatcaagt tggaacgtga actcttggca
    13561 cctttgaaaa atatgagcca aaatgtggtg gatacgactg aactcactcc acgtgaactg
    13621 cgcaaaaccc ttgcagagca gttttcagac caagaacaag cccagtcttt ccgtatcgaa
    13681 gtcatgtctt tcggatttaa gtatggaatc ccgattgatg cggacttggt ctttgatgtc
    13741 cgtttcttgc caaatcccta ttatctacca gaactgagaa accaaacggg tgtggatgaa
    13801 cctgtttatg attatgtcat gaaccatcct gagtcagaag acttttatca acatttattg
    13861 gccttgattg agccgattct gccaagttac caaaaggaag gtaagtccgt tttgaccatt
    13921 gctatgggat gtacgggtgg acaacaccgt agtgtggcat ttgctaaacg cttggcgcag
    13981 gacttatcca agaattggtc tgttaatgaa gggcatcgcg acaaagaccg cagaaaggaa
    14041 acggtaaacc gttcatgaga aaaccaaaga taacggtgat tggtggaggg actggaattc
    14101 ccgtcattct aaaaagtctg cgggaaaaag atgtggaaat cgcagctatc gtgacggtgg
    14161 cagatgatgg tggttcttca ggtgaactcc gaaaaaatat gcaacagttg acaccgccag
    14221 gtgatcttcg taatgtcctt gtggccatgt cggatatgcc taagttttat gagaaggtct
    14281 ttcagtatcg gttctctgag gatgccggag cctttgctgg ccatccattg ggaaatctca
    14341 tcattgctgg cttgtcagaa atgcagggtt caacctataa tgccatgcag ttattgagca
    14401 aatttttcca tacaacaggg aaaatttatc cttccagtga ccatcctttg acccttcatg
    14461 cagtctttca ggatgggaca gaagtggctg gagagagtca tattgtagac catcgaggca
    14521 taattgacaa tgtctatgtg accaatgccc taaacgatga tacgcctctg gccagccgtc
    14581 gagtagtgca gaccatcctt gaaagtgaca tgattgtcct agggccaggt tccctcttta
    14641 cctctatttt gcccaatatc gtgattaagg aaattgggcg ggctcttttg gaaactaagg
    14701 cagaaattgc ctatgtctgc aatatcatga cccaacgtgg ggagacggaa cactttacag
    14761 atagcgacca cgtggaagtc ttgcatcgtc accttggtcg cccttttatc gacactgtct
    14821 tggtgaatat tgaaaaagtg cctcaggaat acatgaattc caaccgtttt gatgaatact
    14881 tagtgcaagt ggaacacgat tttgtaggtc tttgtaagca agtttcgcgc gtgatttcat
    14941 ctaacttcct tcgtctggaa aatggcggtg ccttccacga tggagatttg attgtggacg
    15001 agttgatgcg cattatacag gtgaaaaaat gagtttcaca gtagcagtaa aagaagaaat
    15061 cctgggccaa caccatctga gctggcatga attatctgcc attatcaaaa tgtctggtag
    15121 catcggtctc tcgacttcgg gcttgacttt gtcagttgtg acagaaaatg ccaaactggc
    15181 ccgtcacctc tatgagtcct ttcttcattt ctatgaaatc aaatctgaaa ttcgtcacca
    15241 ccaacggagc aatcttcgca agaatcgcgt ctataccgtt tttacagatg aaaaggtgca
    15301 ggatttgtta agtgatttgc acttggcaga ctctttcttt ggcctggaaa caggtattga
    15361 tgaggcgatt ttatcggatg aggaagcagg tcgtgcctat ctctgtggcg ctttcttggc
    15421 aaatgggagc attcgtgacc ctgagtcagg caagtaccag ttggaaatca gttctgttta
    15481 tctggaccac gcgcaaggaa ttgcctccct tctccaacag tttttactgg atgccaaggt
    15541 gcttgagcgc aagaaggggg ctgtgaccta tctccagcga gcagaagaca ttatggactt
    15601 cttgatagta atcggagcca tgcaggcacg tgatgatttt gagcgggtta agattttgcg
    15661 agaaacccgt aacgacctca atcgggctaa taatgccgag acagctaata tcgctcggac
    15721 agtttctgcc agcatgaaga ccattaacaa tatcagtaaa atcaaagata tcatgggctt
    15781 agaaaatttg ccagtagatt tgcaggaagt ggcgcaactg aggattcagc acccagacta
    15841 ctctatccag cagttagcag atagtctcag cacccctctg accaaaagtg gtgtcaatca
    15901 cagactcaga aaaatcaata agatagcgga tgaattataa agccacgaat cctatgtgac
    15961 tagtggttct tttttataaa ctggtagagt gttttggttg tactttttgt tcagggtcaa
    16021 tgtagttgat agcgttgttg acagcagttg gagcttctcc gaggcctgtc gcaatcagat
    16081 caatttttcc gtcatagtag cagcagtcac cgatagcata gatacctgct tggctggatt
    16141 cctgtttgct gttgacgata atcttgtgac ggttgaggtt gagcccccag tttttaaggt
    16201 taccgacaga agatttgaaa ccatagttga caaagaggtg gtctaggtca atggtttcag
    16261 tttcatcaga tttgactttt gtgatttcaa gtttatcaag tgtttttcca tttccaagga
    16321 gttggctagg ggcgaatggt gtcttgatgg ttacagatga ttcttgcaag gcttgaacac
    16381 tgtgttccaa ggcacggaaa ttatctctgc ggtgaacaag ggtagttggt gcgatttttt
    16441 caaaagccaa agcccaatcc acagccgagt ctcccccacc aagaatcgtc actttcttac
    16501 cagcgtattg ctgaatgtta gaaacgtggt agtggatatt ttcatagccc tcaacacctt
    16561 caagttccag cggacgtggt ttgaaggcac cgccacccat agcgatgatg actgttttag
    16621 tcaggtgact tcctttagaa gttgtgatgg caaattcttc ttcttgtttg tcaatctcaa
    16681 gaaccgtttc attgagatga ataggggtat caaatccatt tagctgttca atcaagcggt
    16741 tagtcaactc ttctccagtc aggtttggga agcctggtac gtctaggatt tccttttcag
    16801 ggtagagaat agcaggttgt ccacctagct ggggaagaga gtcgatgatt tgaaccttgg
    16861 cttggcgtag gtgggcataa aaggctgcaa aaagcccgac aggaccacca cccacaatgg
    16921 taatatcata gagttgagac atagtttctc ctttgttttt tctagtcagt ttattttatc
    16981 atatttttgt tcaatgtaaa atggactagg atagggaaaa aaatgccaga aaatggtata
    17041 ataggacgaa agtgttttga tagggaggat atcagggatg aattttcaac aattatccaa
    17101 tctgcaatat tggaccagtt tgtttgcaag tccatggacg atagctatca atctgattga
    17161 tattttgatt gttgcttata ttttatacca ttttacaaaa gctattgcag gaaccaagat
    17221 tatgattttg gtacgtggag ttttggtgtt tattttagct caaatccttg caaatatgat
    17281 tggtttgact acgatttctt ggttaatcaa tcaaattatt acttatgggg ttattgcggc
    17341 ggttgttatc ttctctccag agattcggac tggtttggaa cgtttgggaa gagcgacaga
    17401 tttcttttcc aatgccccta ttagtgctga ggaacagatg attcgtgcct ttgttaagtc
    17461 tgttgaatac atgagtcctc gtaaaatcgg ggccttggtt gctattcagc gtgtacgtac
    17521 cttgcaggag tatatttcga caggaattcc cttggatgct aagatttctg cagaacttct
    17581 cattaacatt tttattccca acactcccct acatgatggt gcggtgatta tcaaagaaga
    17641 acgtatcgct gtgacgtctg cctatctgcc cttgacaaaa aacacaggga tttccaagga
    17701 atttgggacc agacaccggg cggctatcgg tttatcagaa gtctcagatg ccttgacttt
    17761 tgtcgtatca gaggaaacgg gaggaatttc gataacctat aatggaaggt ttaagcacaa
    17821 cctaacactt gatgaatttg aaacagaatt acgtgaaatc ttacttccaa aagaggaagt
    17881 gggtcttagt tttaaagaac gattgctagg aggatggaaa catgaaaaaa aatagtttat
    17941 atatcatatc ctcactcttt tttgcttgtg tcttatttgt ctatgctacg gcgacgaatt
    18001 ttcaaaacag taccagtgct aggcaggtaa aaacggaaac ctatactaat acagtaacaa
    18061 atgtccctat tgacatacgc tataatagtg ataagtattt tattagcggt tttgcttcag
    18121 aagtatcagt ggtcttgact ggtgcaaatc gcctatcgct agctagtgaa atgcaagaaa
    18181 gtacacgtaa attcaaggtt actgctgacc taacagatgc cggtgttgga acgattgaag
    18241 ttcctttgag cattgaagat ttacccaatg ggctgaccgc tgtggcgact ccgcaaaaaa
    18301 ttacagtcaa gattggtaag aaggctcaga aggataaggt aaagattgta ccagagattg
    18361 accctagtca aattgatagt cgggtacaaa ttgaaaatgt catggtgtca gataaagaag
    18421 tgtctattac gagtgaccaa gagacattgg atagaattga taagattatc gctgtcttgc
    18481 caactagcga acgcataaca ggtaattaca gtggttcagt acctttgcag gcaatcgatc
    18541 gcaatggtgt tgtcttaccg gcagttatca ctccgtttga tacaataatg aaggtgacta
    18601 caaaaccagt agcaccaagt tcaagcacat caaattcaag tacaagcagt tcatcggaga
    18661 catcttcgtc aacgaaagca actagttcaa aaacgaatta aaaatagaaa aggattttat
    18721 aacaatgggt aaatattttg ggactgatgg agtccgtgga gaagctaacc tagaactaac
    18781 accagaatta gcctttaaac taggacgttt tggaggctat gttcttagtc aacatgaaac
    18841 ggaagcgccg aaagtctttg taggacgtga cacacgtatt tcaggggaaa tgctggaatc
    18901 ggccttggtg gcaggtctcc tttcagtagg gattcacgta tacaaacttg gtgtccttgc
    18961 aacaccagca gtagcttact tggttgaaac tgaaggagca agtgccggtg tcatgatttc
    19021 tgctagccac aacccagccc ttgataacgg aatcaagttc tttggcggtg atggcttcaa
    19081 actagatgat gaaaaagaag cagaaattga agccttgcta gatgctgagg aagacactct
    19141 tcctcgtcca agtgcagaag gcttaggaat tttggtagat tatccagaag gcttgcgtaa
    19201 gtatgaagga taccttgttt caactggaac tcctcttgat ggaatgaagg ttgccttgga
    19261 tacagctaat ggagcagctt ctaccagtgc ccgtcaaatc tttgcagacc ttggtgccca
    19321 attgacggtt atcggggaaa caccagacgg tcttaacatc aaccttaatg ttggttcaac
    19381 acatccagaa gcccttcaag aagtggtcaa agaaagtggg tcagctattg gtttggcctt
    19441 tgatggagac agtgaccgct tgattgctgt tgatgagaat ggtgacatcg tcgatggtga
    19501 caagattatg tacatcatcg gaaaatacct ttctgaaaaa ggacaattgg ctcaaaatac
    19561 aattgtgaca actgttatgt ctaaccttgg tttccacaag gccttgaatc gcgaaggtat
    19621 taacaaggca gttactgcag ttggtgaccg ctacgttgtt gaagaaatga gaaaatcagg
    19681 ctacaacctt ggtggtgaac agtctggtca cgttatcttg atggattaca ataccacagg
    19741 tgatggtcaa ttatcagcag ttcaattgac taaaattatg aaggaaactg gtaagagctt
    19801 atcagagttg gcggcagaag taacgattta tccacaaaaa ttagttaata tccgagtgga
    19861 aaacgtcatg aaggaaaagg ccatggaagt gccagctatc aaggccatca tcgagaagat
    19921 ggaagaagaa atggcgggga acggccgtat ccttgttcgt ccaagtggaa cagagcccct
    19981 cttgcgtgtt atggcagaag cgcctacaac agaagaagtg gactactatg ttgataccat
    20041 cacagatgta gttcgtgctg aaattgggat tgactaaatc ctagaaaact agatgaaaat
    20101 aaaaaattat ggtacaatag cttataatat tgtagccgat ggagaaagac atgaatctta
    20161 aacgtgaaca agaatttgtt agtcagtatc attttgatgc acgtaatttt gaatgggaaa
    20221 atgaaaatgg agctcctgaa accaaggtgg atgtgaattt ccaattgctc caacatgacc
    20281 aagaaaatca agtgacttcc ttgattgtca tcttgagctt tatgattgta tttgataagt
    20341 ttgtcattag tggaacaatc tcacaagtaa accatatcga tggccgtatt gtcaatgaac
    20401 caagtgaatt gaaccaagaa gaggtggaaa ccttggctcg tccatgtttg aacatgctca
    20461 atcgtttgac ttacgaagtg actgaaattg cattagactt accaggaatt aatttggagt
    20521 tctaatatga aattagctgt tttcacagat tcctctgctt atctcagtga agagaccttg
    20581 caaagagaag acttgtttgt cttggatatt cctgtcaata ttgatggtga ggaatatgtc
    20641 gaaggcatca atctgtctgc tgaggaattt taccaaaaaa tggctcaggc ttctgaattg
    20701 cctaagacca gtcaaccaag tattgccaag ttagatgaga tcttaacttc gctcaaagaa
    20761 caaggctata cacatgcctt ggggcttttc ctatcttctg gaatttcagg tttttaccaa
    20821 aatatccagt atatggtcga tgactatgag ggcttaacca ttgctttccc agacactttg
    20881 attacaagtg ctcccctagg catcatggtt gaaagcgtct ttaattggcg tgatcagggc
    20941 gatgattttg ccagcattca ggataagcta gccattcaaa tcagccgtac gtcagccttt
    21001 atcatggtag atgacttgga tcatttggta aaaggtggac gcctttcaaa tggggctgcc
    21061 attttgggca atttgcttag cattaagcca atcctttatt ttaacgatca aggtgtgatt
    21121 gaagtttacg aaaaagttcg tactgaaaag aaggccacca agcgcttaat tgaaattatc
    21181 aaggaaacaa cggcttcagg tcaataccgg gtcattgtca ttcatgggaa tgctcctgaa
    21241 aaggctgaag aattgcgtca gcacttgctt gattttggct tgggttcgga tgtttcactt
    21301 gctacatttg gtagtgtcat tggaacgcac ctaggagcag gaagtattgc tctgggttat
    21361 attccagtga tttagttggc gcttctaggc tagttaagat gccttgtatc ttagaaattg
    21421 aacacggact acctgcctct tgaaaaaaga tgcctgtctt gcctttcgtg gaaagtcagt
    21481 gccattccct atttttcaga ggcagctaag gtcctttgta tcttgataat tgaacacgcc
    21541 tggaaccctg tgtgaaaaag atagttcttc caagaagtag acacttcttg attggaattc
    21601 ctattttcac tttgtgttct tacggacttt gtatcttaga caggagtaga gatgagtatt
    21661 cgagtaatta ttgccggttt taagggaaag atgggccagg ctgcttgtca gatggtattg
    21721 actgatccag acttggactt ggtggcagtt ttggatcctt ttgagtctga gtcagaatgg
    21781 cagggtattc ctgttttcaa ggataaggct gatttagctg gttttgaagc ggatgtctgg
    21841 gtagatttta ctactccagc tgttgcctac gaaaatacac gttttgctct tgaaaatggc
    21901 tttgctccag tagttggaac gactggtttc acgagtgaag aaattgcaga gctaaaagaa
    21961 ttttctcgtg cccaagactt gggtggcctg attgccccta actttgcctt gggtgctgtc
    22021 ttactcatgc aatttgcgac gcaggctgcc aaatatttcc caaatgtgga gattattgag
    22081 ctccatcatg acaggaaaaa ggatgctccg agtggaacag ccattaaaac agctgagttg
    22141 atggcagagg ttcgagagtc cattcagcaa ggcgcagcag atgaggaaga gctgattgct
    22201 ggtgctcgtg gtgctgactt tgatggtatg cgcatccact cagttcgttt gccaggcttg
    22261 gtagctcatc aggaagtcat ctttggcaat cagggagaag ggctgaccct ccgtcatgac
    22321 tcctatgatc gcatctcctt catgacagga gtcaatttgg gaattaaaga agttgtcaag
    22381 cgtcatgagc ttgtctatgg attagaacac ttattatgag attaacgcaa atgccttctg
    22441 aatttcagaa ggctttacca gtattagaaa aaattaaaga agcaggcttt gaggcttatt
    22501 ttgttggggg ctctgttcga gatgcccttc tccatagccc tatccacgat gtggatattg
    22561 cgacgtcttc ttatccagaa gagaccaagc agatttttcc gcgaacagcc gatatcggaa
    22621 tcgagcatgg aaccgtcttg gtcttagatg gggatgagga gtatgaggta acaacctttc
    22681 ggacagagga tgtctatgtg gactatcgca gacccagtgc ggtttccttt gtacgatcgc
    22741 tagaagaaga cctcaaacgc cgtgatttca cagtcaacgc ctttgccttg gatgagacag
    22801 gagaaatcgt tgacttgttc catggtttag aagatttgga aaagcaagtc ttgcgagcag
    22861 ttggagtggc tagtgagcgt ttcaacgaag atgctttacg gattatgcgt ggtttccgtt
    22921 ttcaggctag tcttggtttt gcacttgagc cagaaacatt taaagctatg aagaccttga
    22981 cgccgctttt ggagaaaatt tctgtagagc gtaccttcgt cgagtttgat aaactcttgc
    23041 tggctccatt ttggagaagg ggcttggctt ccatgattga gagtcaagct tatgactatc
    23101 tccctgatat ggcatctagc caggacaagc tcaacagact gtttgatttg gagactgatt
    23161 ttacttttga atcctctgaa caagcctggg cggctttact atgggctttg gagattgaaa
    23221 atgcgcagtc acttttgaaa tcttggaaga cctcacgcca gtttgccaag caagttcagg
    23281 atttgctgat tattttggct ctgcgtgaaa atggagaatt gagcaagcga gattgttatc
    23341 gctttgacat agatttgctt ttacaggctg aaaatcttcg tcaagctcaa ggaaaagaag
    23401 tcaacccaca agccatcaca gaaaaatacc aaagcttgac tatccatgac aagaaagaga
    23461 ttcagattaa tggcggtatt ttgatcaagg aatatggcta tcagccaggc ccagacttgg
    23521 gagagatttt aacagagatt gagtttgcca ttgtcgatgg agaattggag aataatcgtg
    23581 aagccatcca tgcttacctg agggagaaaa aatgagtgat tttatcgttg aaaaactaag
    23641 taaatccgtt ggtgacaaga ccgtttttag ggatatttcc tttattatcc atgacttaga
    23701 cagaattggt ttaatcggtg tcaatgggac tggcaagacc acccttttgg acgtcctttc
    23761 tggtgtttct ggatttgatg gggatgtcag tcctttttca gctaaaaatg attaccagat
    23821 tggttacttg actcaggatc ctgattttga tgatagaaag acagttttgg atacggttct
    23881 atctagtgaa ctcaaggaaa tccagctcat tcgtgagtat gaattgatta tgctcgacta
    23941 tagtgaggac aagcaggcgc gtttggaacg tgtcatggca gagatggact ctctccaagc
    24001 ttgggaaatc gaaagtcagg tcaagaccgt tcttagcaaa ttgggcattc aagacttatc
    24061 tactcctgtt ggggaattgt caggtggtct gagaagacgg gtacagttgg cacaagtctt
    24121 acttggcaac cacgacctct tgcttttgga tgagccgacc aaccatctgg atattgcgat
    24181 tattgagtgg ctgaccctct ttttgaaaaa ttctaagaag accgtccttt ttatcactca
    24241 cgatcgttat ttcttagacg ctttgtcaac acggattttc gagttggatc gtgcaggctt
    24301 gaccgagtac cagggaaatt accaggacta tgttcgccta aaggcggaac aggatgagcg
    24361 cgacgcggct cttcttcaca aaaaagaaca actctacaaa caagaattgg cctggatgcg
    24421 cagacaaccg caggcgcgtg cgaccaagca gcaggcccgt atcaatcgat tccatgatct
    24481 gaaaaaggaa gtttcaggca gtagtgctga gacagacttg accatgaact ttgaaaccag
    24541 tcggattggg aagaaagtca tcgagtttca ggctgtttcc tttgcctatg aaaacaagcc
    24601 tattttgcaa gattttaatc tcttggtgca ggccaaagac cgtatcggaa tcgttgggga
    24661 caatggtgtt ggaaaatcaa ccctgctcaa cttgattgca ggaagtcttg agccaactaa
    24721 aggacaagtt gtgattgggg aaactgttcg catcgcctat ttctctcagc aaattgaggg
    24781 tttggatgaa agcaagagag ttatcaatta cctgcaggaa gtggcagaag aggtcaagac
    24841 cagtggcggt tctacgactt cgattgcaga gttgctggag cagttccttt tcccacgttc
    24901 gacgcatgga accttgattg agaaattgtc agggggtgag aaaaaacgtc tttatctcct
    24961 caaactgctt ttggaaaaac caaatgttct tcttttagac gagccaacca atgacctaga
    25021 tattgcaact ttgacagtct tagagaattt cttgcaaggt tttgcaggtc ccgttttaac
    25081 agtcagtcac gaccgctatt tcttggataa ggtagcgacc aagattctcg cttttgagga
    25141 tggcaagatt cgtcctttct ttggtcatta caccgactat cttgatgaaa aagcttttga
    25201 aacagatatg gccaatcaag tgcaaaaggt cgaaaaggaa aaagtggtca aggttcgaga
    25261 agacaagaaa cgcatgacct accaagaaaa gcaggagtgg gcaagtattg aaggtgatat
    25321 tgaaaccttg gaaaaacgta tcgctgctat tgaagaggaa atgcaggcta acggctctga
    25381 ctttggtaag ctggctactc tccaaaaaga attggatgag aaaaatgaag cactccttga
    25441 aaaatacgaa cgctatgagt atctcagtga atttgatagt taagactatt cgaaaatcaa
    25501 agtgcaaact atgtcggctt cacattgccg actcaagtac agcctgtgac cggcttccta
    25561 atttgcactt tgatttttat tgagtataaa agaatagaaa aatcccgtct catgaaagga
    25621 tttctctatt cttttttggt ttcttgatga aagatatttt gccaggtttc gtggcgacgc
    25681 cagatactgg tatggatgat accatctaac tcatagcaga tgagtttggt tttttgactg
    25741 atggaagtaa tttgaatgtc ctttatagca gaatttaact cttttttggt cttataagcc
    25801 tctttatcca tctgctctcc atcttggcga atatagacaa aatcatcagt caagagttct
    25861 tctagttggt ttccttggtc aatcaattgt tcacgcataa gcaatttttt atagacattg
    25921 tctaaaattt catcctcagg gataggtgct ggttcgatat ggacatcggt atcaaagacg
    25981 ccaaaacgct cctccagcat agactcgacc tgatccgcga tttcatggct ttcaaaaaca
    26041 gacaagtcag gattcatctc tagtgtaata tccaggtaga tgttgctacc gtaggtgcga
    26101 cctctttgcg atttgacctt gctgattttg ggaatttcca tgatagcctt ttggtagtcc
    26161 tcgagcaggc ggtcgtcaaa gccatctgaa agactaaagg aagactcgat gaagatatca
    26221 taggcagtct tcaagataaa gaaagtgatg atgatagcaa ccagtttatc cacaatcgga
    26281 taattgaaac tactagctag gatggcaatg gcggttccaa gtgaggtaac agcgtcagaa
    26341 agattgtcct tagcagctgc cttcagcgcc ttggagttgg atttcttact gaggcgagta
    26401 ttgtagagat agaccacaaa cataatcgct gcagaaatga ttcctagagt tgcaccaaga
    26461 ggatcaatga ccgtttcttc ccgactgaga atcttttgaa tggtatctct tagaacatcg
    26521 aaaccgacat agaacatgat gatagaagtg atcaagcttg ccaaatcttc aatcttccaa
    26581 tgaccaaaac ggtggtcacg gtctgcaggc tggcgcgcca tccgaatccc gattaagagg
    26641 gccacatttc caatgatgtc cgatacgtta ttaaaaccat cggccaccaa actggatgaa
    26701 tgaaggagat gaccagctgc taatttagct gcagacaaga tcaaataggt cgaaatactg
    26761 ataatggctc cacgctcagc taacttgaga tttgagatag attgcttcat tcaacttcct
    26821 ttctagtcac atagcattct accattatac tggttttcaa ggaaaaattc aaataaggca
    26881 gtttttactc tttgaaaact ccccaaaatt tggtataatg gtactactca agggagtagc
    26941 tggcagaaac ctgtgatagt gtcgtcattc cgaattttat gctgaaaagt atgctttccg
    27001 gccctatctt aaacagcgag acttgttatg attaacaggt ctctttttgt ttgtcgtgaa
    27061 aagatatgac agaaagagag tctgttttgc acacaatgtc aaggaggaga cacatgtcaa
    27121 aagaacaaaa acgccaagcg ttttatactc agagccctga agaggtcttg caggctgtgg
    27181 atgcgaccga gcaaggtttg tcatcaagtg aggcggaaaa gcgccttgcc gaatttgggc
    27241 acaatgaact cgaagaaggc gagaaacgat caatcttggt caaattcatc gagcaattta
    27301 aggatttgat gattatcatc ctagttgcgg cagcaatctt gtcagtcgtg acttctggtg
    27361 gggaagatat cgcagatgcc attatcatcc tagctgtggt tatcatcaac gctgcctttg
    27421 gtgtttacca agaaggaaaa gctgaagaag ctattgaagc cctcaaatcc atgtctagtc
    27481 cagttgcccg cgttcttcgt gatggacaca tggcagagat tgactctaaa gaattggtac
    27541 caggcgatat cgttgccctt gaagcaggtg acgtggtacc agcggaccta cgtttgatag
    27601 aagccaactc tcttaaaatt gaagaagcag ccttgacagg tgaatctgta ccagtcgaaa
    27661 aagacttgtc agtcgagctt gcgacagatg ctggtattgg tgaccgtgtc aacatggcct
    27721 tccaaaactc aaacgtaacc tatggtcgtg ggatgggtgt tgttgtcaat acaggtatgt
    27781 acactgaagt tggtcatatt gctggtatgc ttcaagatgc ggatgagact gatacaccac
    27841 tcaaacaaaa cttgaacaac ctttctaagg tcttgaccta tgctatcttg gtcattgccc
    27901 ttgttacttt tgtagtgggt gtcttcattc aagggaaaaa tccacttggt gagttgttga
    27961 cttctgttgc ccttgccgtt gcagccattc cagaaggact tcctgctatc gttaccatcg
    28021 ttctttctct tggtactcaa gttttggcca aacgacattc catcgttcgt aagttgccag
    28081 cagttgaaac acttggttca actgaaatca tcgcttctga taagactggt acgctgacta
    28141 tgaacaagat gacagtcgaa aaagtcttct acgatgcggt tctacatgac tcagctgatg
    28201 atattgaact aggtcttgaa atgccactac ttcgttcagt tgtcttggcc aatgatacga
    28261 aaatcgatgt ggaaggtaac ttgattggtg acccaaccga aacagccttt atccaatatg
    28321 ccttggacaa gggctatgat gtcaaaggtt tcttagagaa atatcctcgt gtagctgaat
    28381 tgccatttga ctctgaccgt aagctcatgt caacagttca cccattgcca gatggtcgtt
    28441 tccttgtagc agtcaagggt gcgccagacc aactcttaaa acgttgtctt cttcgtgata
    28501 aggctgggga tattgctccg attgatgaga aggttacaaa tctcattcat acaaacaatt
    28561 ctgaaatggc tcatcaagcc ttgcgtgtcc ttgcaggtgc ttataagatt atcgatagta
    28621 ttccagaaaa tctcacttct gaagagcttg aaaatgattt aatttttact ggtttgattg
    28681 ggatgattga ccctgaacgt cctgaagccg ctgaggctgt tcgtgtggct aaggaagcgg
    28741 gaatccgtcc aattatgatt acaggtgacc accaagacac agcggaagcc attgccaaac
    28801 gtttgggaat cattgacgca aacgatacag aaggtcacgt tttaactggt gctgaactca
    28861 atgaactgtc agatgaagaa tttgaaaaag tcgttggtca atactctgtt tatgcccgtg
    28921 tgtctccaga acacaaggtt cgtatcgtca aggcttggca aaaacaaggt aaagtcgttg
    28981 ccatgacagg tgacggtgtc aatgacgcgc cagctctgaa aacagccgat atcggtatcg
    29041 gtatgggaat cactggtaca gaggtttcta agggggcttc tgatatgatt cttgcagatg
    29101 ataactttgc gactattatc gtcgcagtgg aagaaggacg taaggtcttc tcaaacattc
    29161 aaaagactat tcagtaccta ctttctgcta atactgctga agtattaacc atcttcctat
    29221 caaccttgtt tggttgggat gtcttacagc cggttcatct tttgtggatc aacttggtaa
    29281 cggatacctt cccagctatc gctcttggtg ttgaacctgc agaacctggt gtcatgaatc
    29341 ataaaccacg tggacgcaag gcaagcttct tctcaggtgg tgttttgagt tctatcattt
    29401 atcaaggtgt actccaagca gctcttgtta tgagtgttta tggccttgcg attgcttacc
    29461 cagttcatgt gggtgacaat catgctattc atgcagatgc cctaacgatg gcctttgcaa
    29521 cccttggttt gattcagctc ttccatgcct acaatgtcaa gtctgtctac caatccatct
    29581 tgacagttgg cccattcaag tctaagacct ttaactggtc catcttggta tcctttatcc
    29641 ttctcatggc aacaatcgtt gtagaaccgc ttgaaggaat cttccacgta accaaactag
    29701 acttgtcaca atggggaatt gttatggctg gaagcttctc aatgattatc atcgtcgaaa
    29761 tcgttaagtt tattcaacgc aaactcggtt ttgacaagaa tgcgatttaa gggaggtttc
    29821 ggatgtcagc atatcaatta ccgaccgtat ggcaggatga agctagtaat caaggagctt
    29881 ttacggggct aaacagacca acagcaggtg cccgtttcga acaaaacttg ccaaaaggag
    29941 aacaagcttt tcagctttat tcactgggaa caccaaatgg tgtgaaggtt actatcttat
    30001 tggaagaatt actagaagct ggttttaagg aagcggctta cgacttgtat aagattgcta
    30061 tcatggatgg ggatcaattc ggctcagact ttgtgaagct caatccaaat tccaagattc
    30121 cagccttatt ggaccagtca ggtactgaaa acgtaagagt ctttgagtct gctcatattc
    30181 ttctttacct tgctgagaaa tttggagcct ttttaccaag taatcctgtg gaaaaggtag
    30241 aagttttgaa ttggctattc tggcaagcag gtgcagcacc ttttctaggt gggggatttg
    30301 gacatttctt caattatgct cctgaaaaat tggaatatcc tattaaccgt tttacgatgg
    30361 aagtgaaacg ccagttggat ttattggata aggaattggc tcagaaacct tatattgcag
    30421 gcaatgacta tacgattgca gatattgcta tctggtcttg gtatggacag ttagttcaag
    30481 gaaatcttta ccaaggttct gcaaaattct tggatgcctc aagttatcaa aatctagtaa
    30541 aatgggcaga aaaaattgcc aatcgtccag ctgttaagcg tggattggaa gtaacttata
    30601 cagaaattaa atagcaaaaa agtcatcttc ggatggcttt ttgctacagt tgagggaaag
    30661 ggtttgcagt tatcggcttt tgtgctataa ttgctataat atagtaaaaa gcaagaggag
    30721 tgtgaggaag tggaaaagaa aattgtgaag gatatcttat ttttatctca agtgtctcag
    30781 ccggcaagtc aggaggacct ttatcttgcc agagatttgc aggatacact cttagcaaat
    30841 cgtgatacct gtgttggtct agctgccaat atgattgggg tgcagaagcg cgtgattatc
    30901 tttaatcttg gcttggttcc cgtggtcatg tttaacccag tgcttctgtc ctttgaagga
    30961 tcttatgagg cagaagaagg ctgtttgtcc ttggtaggtg tgagatcaac taagcgttat
    31021 gaaaccataa ggcttgccta tcgtgacagc aagtggcagg aacagaccat taccttgaca
    31081 ggcttcccag ctcagatttg ccagcatgag ctggatcact tggaaggacg aatcatttag
    31141 gaggaaagca aatgaaacga atagtctttg aacttatttt tatcgcaacg acctggtata
    31201 tctttttacc gccccttaac ctgaccagct gggaatttct cttcttcctc tgtgggcatt
    31261 tgttagttgt ggcaatctta tttggctttg gcaaggggat aaaccttgtc aaaacggttc
    31321 atgtgcgcca cggtaaggcg gaagctgcct taaatcttga gggtttcaaa atcaatcggt
    31381 tagggaaaat tctgttagct tcgattggag gaattcttct cttggcagct ttggtttcct
    31441 tggtaacttc cagcatgttt caggctaaaa attatgccaa tgtagtcaca gttacggaaa
    31501 aagactttac tgaatttcct aagagtgaca ccagtaaggt tcctatccta gatagaagta
    31561 ctgctgaaaa aattggagac cgctacttgg gttccctaac cgataaggtg tcgcaatacg
    31621 tagcggcaga tacctatacc caattgacaa ttgatgggaa accttatcgg gtcacaccac
    31681 tagaatatgc agaccctatc aaatggttta acaatcaagc caagggaatc ggtgagtata
    31741 ttaaggtgga catggtaact ggaaatgcgg atttggtgga cttgaagaca ccaatcaagt
    31801 attcagactc ggagtatttt aaccgtgatg tcaaacgtca cctgcgcttg aagtacccga
    31861 ccaaaatctt taaaactcca tcttttgagg tggacgatga gggcaatcct ttctatgtag
    31921 caacggttta ccaaaagcaa tttggacttg ctgttcctcg tcctgcttca gtcattatct
    31981 tggatgctac aaatggagaa accaaggaat acagcttatc agatgttcca gaatgggtgg
    32041 acaggatcta tccagcagag gaaaccattg agcaaatcaa ctacaacggc aagtacaagg
    32101 acggtttctt gaatgccatg atttccaaga aaaacgtgac ccagactacc aatggctata
    32161 attacttgtc tatcggtaat gacatctatc tctacacagg tgtgacgtcg gctaatgcgg
    32221 atgagagtaa tcttggtttc atccttgaaa atatgcgaac aggagaaatc actaagtata
    32281 gcttggcttc tgcgacagaa gaatcagccc gtgaatcagc agaaggtgct gttcaggaga
    32341 aatcctacaa agcaaccttc ccaatcctca tcaacctcaa tgacaagcct ctctacatca
    32401 tgggcttgaa ggacaatgct ggcttggtca aagagtacgc cctggtagac gcagtcgagt
    32461 accaaaatgt tatcgttgct actacagtgg aagagatgct cagcaagtat gccaataaaa
    32521 acgaccttga aattgacaat gcaacgacag aaagcatcaa gggagtagta gcagacctca
    32581 aatcagctgt tatcaaggga gacactgtct acttctttaa agttgatggc aagatataca
    32641 aggtcaaggc ttcagtatcc gatgaccttc cttaccttga aaatggtaaa accttcgaag
    32701 gtcaagtagg aaaagacaat tatctcaaga cctttaagct acggtaaaaa taggtttttt
    32761 cagaaagtat atgttataat agagtaaatt aagactaatg gaggacaaag aaatgggttt
    32821 ttatttgatg gttgcttcaa tgctactcgg actgctcgct ttgaagatag gtttttctca
    32881 gttcaaggag aaaaaagata aattcttatc tatcttaaca agtttagctg gcacagccct
    32941 tgttctcgtg gctgtctggt taggatggcc caaataaata gaaaaacctc ggtcaaaccg
    33001 aggtttttca aatgaatttc ttggttgtgg ctaaaaaata tgctacacta tcaatatgaa
    33061 aattttaatc ccaacagcaa aagaaatgaa cacagacttg ccaagtatcg aggcaattcc
    33121 tttaaaacca gaaagtcaga ccgtgcttga tgccttggct ctctattctg ccagtcaatt
    33181 ggagagtttc tacaaggtat cagctgagaa agcggcggaa gaatttcaaa atatccaagc
    33241 tttgaaaagg caaactgctc aacactatcc agccttgaaa ctttttgatg ggcttatgta
    33301 ccgcaacatt aagagagata agctgaccga ggcggaacaa gattatcttg aaaatcatgt
    33361 cttcattacc tcggctttgt acggtgttgt tccagccttg tcacccatgg ctcctcaccg
    33421 tttggatttt ttgatgaaat taaaagtcgc tggtaagact ttgaagagcc attggaaggc
    33481 agtctatgat gaagctctga agaaggaaga agtgattttc tctctcttgt catcagagtt
    33541 tgagactgta ttttctaagg aaatcagagc aaagatggtg accttcaaat tcatggagga
    33601 tagaggcggt cagctgaaga ttcactcaac tatctccaag aaagcgcgcg gggcctttct
    33661 aacagcttta atagaaaatc aagtacaaac tgtgggggaa gcacgtcgct tgaactttgc
    33721 tggatttgtt taccgagaag atttgtcaca accacaggga ttggtttttg taaaggaagt
    33781 atagaaatca gataagagaa aagtcagcac gattatgctg acttttttga taacttataa
    33841 agtattggac aaatgaatct catttgataa ggtgttaaga tgattaacca aatttatgat
    33901 tgacagatga tttcggcaaa taaacttctt ggtaatccct ggaatataga aattagacac
    33961 gatgatatcg taatctgtct gatttaaaat ctcaggactt gtttttaatt catcccaaat
    34021 atcgaaggta aagcggttat tacagtaata ggaaagcata tcaacaaagg taagggatat
    34081 agcatggtca aagttactga taatcaaaac cttgataggt ggtcgatttt ctaacaactg
    34141 agtagagagg ttttcagcat gggttaagat ggtgtacatc aagtgttcca actgttcagg
    34201 atggtcatgc tgtccaattg cctgacggta ttgagccaat tcttgacgcg cacttcccat
    34261 aaagtctggg aagtaaactt caaatttttt aatcgtcaat gctttttgtt caaataagat
    34321 tggggtagaa aagatttcct gtctttcaaa gaaagcagtg ttgtgtaaat gccaaatcag
    34381 ctcatcgtgg ttagtaaaag taatcttata ttgttttgat aggcgttcta atatttgact
    34441 aagtaattgg taagagtatc ttgcttgact gttatcttct aaagagttga agaattcttg
    34501 aggatctagg aaaatatcat tctgaagaaa agaaataaaa atttgagcga ttgtatctgg
    34561 agtcatttca agcccaaaat gtttagagaa ataagcaagt gtctcctgga aatttggaat
    34621 gctctttaaa agcggataat attctttgta aaagtggttg gggaggtcaa tgaagtgacc
    34681 atttttaacc ctgtggatac tgatagctat catcagtttg tacattctat aaattgaaaa
    34741 tcgcattgga taattcgtga ttttatagaa gaagtcagca aactctgtta aatcttcctc
    34801 aggaaggtca ggaaaaggcc aatctaggaa ataatagcgc tcagaaaagt attgggcaaa
    34861 aaagtaacga atatcgatct catttccacg tatctcggat ggagtaaaag ataattcaat
    34921 ttgaaactga gatgaaagaa cctttgtaat atttctgccc agtcgataga ggttggcact
    34981 actaaagtaa agattttcta ttgttcgata aataggtagc ccctcattga agaacatata
    35041 ctctagtaat tgaaaagatt gagaattagc aagaaaatat tgataaatat cttctacact
    35101 agtgttaact tctaaatcta tcatgattcc gttaacagaa gattggatgt taatagatgg
    35161 aaaggcaata cgtaattcat ttaaatcact ttttaagatt cgttctgtgc atcctaattt
    35221 ttcggctagt tcatgtaatt tcatccaatt acgattctga attaggtttt ccattaagcg
    35281 taattgtctt tgaacttttg tggataaaag gtttctcata cgggtatttt ctccataatt
    35341 cattatatct ttttttgtct aagtagtata aaaaaattag agcaagaaaa ttgtcgttag
    35401 taatcggaaa aattagaata atataagtgg cttttaagtg caaaaacaga atatattaat
    35461 tgttcagaaa atattgatta atgcctcaaa tagttgtaca ttgtaaacta taaaatacta
    35521 t
//
LOCUS       DAVHIT010000011        34458 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVHIT010000011 DAVHIT010000000
VERSION     DAVHIT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.011"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(1..459)
                     /locus_tag="VVN16_000429"
                     /pseudo
     CDS             complement(1..459)
                     /locus_tag="VVN16_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            524..820
                     /locus_tag="VVN16_000430"
     CDS             524..820
                     /locus_tag="VVN16_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU5571875.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGVLKRSKKKSFYWYKDVIDSNGASIE"
     gene            941..1885
                     /gene="manA"
                     /locus_tag="VVN16_000431"
     CDS             941..1885
                     /gene="manA"
                     /locus_tag="VVN16_000431"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU5571876.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDTLLTKVPVKAG
                     DFFYVPSGTMHAIGTGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1906..3237)
                     /locus_tag="VVN16_000432"
     CDS             complement(1906..3237)
                     /locus_tag="VVN16_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU5571877.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMMP
                     LLFIVLPQLFDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILG
                     ILTFVFGIPSALSYGVMADVHIFGKIFFDAMDFLASNLLMPFGALYLSLFTGYIFKKA
                     LAMEELHLDERAWKQGLFQVWLFLLRFFVSSFQSSSLWSSLPNLCNQKGLE"
     gene            3320..4074
                     /locus_tag="VVN16_000433"
                     /pseudo
     CDS             3320..4074
                     /locus_tag="VVN16_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4094..4549)
                     /locus_tag="VVN16_000434"
                     /pseudo
     CDS             complement(4094..4549)
                     /locus_tag="VVN16_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            complement(4569..5744)
                     /locus_tag="VVN16_000435"
     CDS             complement(4569..5744)
                     /locus_tag="VVN16_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5571878.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5803..6648)
                     /locus_tag="VVN16_000436"
     CDS             complement(5803..6648)
                     /locus_tag="VVN16_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5571879.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6773..7330
                     /locus_tag="VVN16_000437"
     CDS             6773..7330
                     /locus_tag="VVN16_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5571880.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7349..7816
                     /locus_tag="VVN16_000438"
     CDS             7349..7816
                     /locus_tag="VVN16_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU5571881.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7905..8534
                     /gene="upp"
                     /locus_tag="VVN16_000439"
     CDS             7905..8534
                     /gene="upp"
                     /locus_tag="VVN16_000439"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU5571882.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLNLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8708..9298
                     /gene="clpP"
                     /locus_tag="VVN16_000440"
     CDS             8708..9298
                     /gene="clpP"
                     /locus_tag="VVN16_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU5571883.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9377..9625
                     /locus_tag="VVN16_000441"
     CDS             9377..9625
                     /locus_tag="VVN16_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU5571884.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9727..10887
                     /locus_tag="VVN16_000442"
     CDS             9727..10887
                     /locus_tag="VVN16_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5571885.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11155..12024
                     /locus_tag="VVN16_000443"
     CDS             11155..12024
                     /locus_tag="VVN16_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5571886.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12028..12984
                     /locus_tag="VVN16_000444"
     CDS             12028..12984
                     /locus_tag="VVN16_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5571887.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12984..13748
                     /locus_tag="VVN16_000445"
     CDS             12984..13748
                     /locus_tag="VVN16_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571888.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13748..14458
                     /locus_tag="VVN16_000446"
     CDS             13748..14458
                     /locus_tag="VVN16_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571889.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISNRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14872..15528
                     /locus_tag="VVN16_000447"
     CDS             14872..15528
                     /locus_tag="VVN16_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5571890.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNYQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15636..16731
                     /gene="prfB"
                     /locus_tag="VVN16_000448"
     CDS             join(15636..15707,15709..16731)
                     /gene="prfB"
                     /locus_tag="VVN16_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU5571891.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16749..17441
                     /gene="ftsE"
                     /locus_tag="VVN16_000449"
     CDS             16749..17441
                     /gene="ftsE"
                     /locus_tag="VVN16_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5571892.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17434..18360
                     /locus_tag="VVN16_000450"
     CDS             17434..18360
                     /locus_tag="VVN16_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5571893.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLRI"
     gene            18646..20826
                     /locus_tag="VVN16_000451"
     CDS             18646..20826
                     /locus_tag="VVN16_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU5571894.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20878..21693
                     /locus_tag="VVN16_000452"
     CDS             20878..21693
                     /locus_tag="VVN16_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5571895.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            21833..23176
                     /locus_tag="VVN16_000453"
     CDS             21833..23176
                     /locus_tag="VVN16_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5571896.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKIVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23553..24743
                     /locus_tag="VVN16_000454"
     CDS             23553..24743
                     /locus_tag="VVN16_000454"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5571897.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            25296..26231
                     /locus_tag="VVN16_000455"
     CDS             25296..26231
                     /locus_tag="VVN16_000455"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU5571898.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            26265..27302
                     /locus_tag="VVN16_000456"
     CDS             26265..27302
                     /locus_tag="VVN16_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5571899.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFVDEKIVILDYFMDITTAKKRFLTDDELKLF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNYSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            27475..28080
                     /locus_tag="VVN16_000457"
     CDS             27475..28080
                     /locus_tag="VVN16_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU5571900.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            28237..28767
                     /locus_tag="VVN16_000458"
     CDS             28237..28767
                     /locus_tag="VVN16_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU5571901.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            28788..29690
                     /locus_tag="VVN16_000459"
     CDS             28788..29690
                     /locus_tag="VVN16_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEU5571902.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            29690..30172
                     /locus_tag="VVN16_000460"
     CDS             29690..30172
                     /locus_tag="VVN16_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU5571903.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            30174..31715
                     /locus_tag="VVN16_000461"
     CDS             30174..31715
                     /locus_tag="VVN16_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5571904.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(31771..32574)
                     /locus_tag="VVN16_000462"
     CDS             complement(31771..32574)
                     /locus_tag="VVN16_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5571905.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(32658..32870)
                     /locus_tag="VVN16_000463"
     CDS             complement(32658..32870)
                     /locus_tag="VVN16_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571906.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(33349..33558)
                     /locus_tag="VVN16_000464"
     CDS             complement(33349..33558)
                     /locus_tag="VVN16_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571907.1"
                     /translation="MLSVNTILEKFYKKHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33728..34000
                     /gene="rpsP"
                     /locus_tag="VVN16_000465"
     CDS             33728..34000
                     /gene="rpsP"
                     /locus_tag="VVN16_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5571908.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            34020..34259
                     /locus_tag="VVN16_000466"
     CDS             34020..34259
                     /locus_tag="VVN16_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU5571909.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
BASE COUNT        10493 a         6419 c         7456 g        10090 t
ORIGIN      
        1 cgacaatggt ttgagcgtct gctttcttag gatgttctgg acgtggcgta atatttcgcc
       61 aaccatggcg cttcaacagt tgatagaagg catcacgtgt gtaggaacga cctaactcct
      121 ttttataagc ctgaaataag gcatcaattg taacaaattc tcctgcctct gtagccttca
      181 aatggcgagc aagaaaggct ttctcttcct caactgtcat atatgcatgg ttacgaccac
      241 cacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc atatttttta catttcgcca
      301 aatcgttgtt tgattacagt ctaaaagctc tataatctct ttataagatt tgcccatcag
      361 acgaaataga acgatttgaa ggcgtttatg atatttagct gtacgagagt cttttaaaag
      421 tgttttgatg gtttggattt cttctttagt caatttcata tcactattat ataatgcttt
      481 ttgattttag tttagtatta tcgtagaaaa tggactcggt actatggata caccggatga
      541 gaatggttat gtagcagatg actatcggat tacttactta gaggcccaca tcaaggccat
      601 gcgagatgcc atttaccaag acggggttga cttgcttggt tatacgactt ggggctgtat
      661 cgatccggtt tcagctggca caggggaaat gaataagcgc tatggcttta tctatgtaga
      721 tcgagataat gtaggaaacg gagttctcaa acgtagcaag aagaagtcct tctattggta
      781 caaggatgtc attgatagca atggtgcaag catcgagtag agcacatttg tttactattt
      841 ttataaaatc ttctccctgc tttataagat gtaaaaaaga gttcaaatgg aactcttttt
      901 ttgctataat gaggggagaa aaatcagaca ggagattaag atgtcagaac cattattttt
      961 acaatcagtt atgcaagaaa aaatctgggg tggtaccaag ctacgtgatg agtttggcta
     1021 cgacatccca agtgaaaaag tcggagaata ctgggctatc tcagctcatc taaatggtgt
     1081 ttctaaagtt gccaatggtc gttacgaggg aacagatctt gctactttgt atgcggaaca
     1141 ccgtgaatta tttggcaatc gtccagaacc tgtatttcca cttttgacca agatcctcga
     1201 tgccaacgac tggctcagtg tccaagttca cccagacgat gcttatggac tcgagcatga
     1261 aggcgaactc ggaaaaacag aatgctggta catcatcgct gcggatgaag gttcagagat
     1321 tatctacggt cacaatgcca agtcaaaaga agaactccgc cagcaaatcg aggacaagaa
     1381 ctgggatacc ttgcttacca aagtccctgt caaggctgga gatttcttct atgtaccaag
     1441 tggcaccatg catgctatcg gtactggcat cttgattctt gaaacccaac agtctagtga
     1501 taccacttac cgtgtctatg actttgaccg taaggatgac aaaggcaact tgcgtgaact
     1561 tcatcttgaa aaatccatcg atgtcttgaa cattggtgag ccagcaaata gccgtcctgt
     1621 aactgttaaa gcagatgatt tgcgttccac tctccttgta tctaatgatt tcttcgcagt
     1681 ttacaagtgg gaaattactg gaaaagttga ctttgaaaag acagctgact acagcttatt
     1741 gagtgtctta gctggtcaag gtcagctaac tgttgacggg aaaaattatc caattcaaaa
     1801 agggagacac tttatcctac caagtgatgt tgaagcttgg actctggaag ggcaaggttt
     1861 ggaattgatt gttagccatc cataaaaaga aagggcctga gttcactact caagtccttt
     1921 ttgattacat aaattgggca atgaagacca caatgatgat gattggaatg acgaaacgaa
     1981 gaaacgaaga aggaagagcc agacttggaa cagtccttgt ttccatgctc tttcatcgag
     2041 atggagttcc tccattgcaa gagccttttt aaagatatag cctgtaaaaa gtgaaaggta
     2101 gagagctcca aatggcatga ggagattgga agccaagaag tccatagcgt caaagaagat
     2161 cttaccaaaa atgtgaacat ccgccatgac accgtaagat agggctgaag gaatgccaaa
     2221 gacaaaggtc aaaattccta aaataacact ccatttggca cgtttgctgt tatcctggtt
     2281 ggtgatattg cccacattga tctccagcat cacgacagaa gaagtgacag tcgcaaagag
     2341 gaagaggacg tagaaaatgg ttccaaaagg catcttgtca aagagttgag gtaagacgat
     2401 aaagagcagg ggcatcatga ctgtaacccc tagtgagagg gcaaagaaag attgtcccag
     2461 agcatagagg agaccagcgc tagtcagttt tgaaaagtct ggtttgagga agtaaagaac
     2521 cccttccatg gcatttggca aactgagaga gcgaccgatg ataacaacaa agatgataaa
     2581 gagcaggggc atcatgactt tcgaagctct ttcaatccct ttttgaaccc cacgtgatac
     2641 aataaagata tttaataaga taaaggctgc ttgagctact agggcaatgg ctggatttga
     2701 aatgattgaa gtaaataact gagcataatc acccgttcca ccaagttgga acaatttccc
     2761 aaactcaata cctagataga ctagaatcca tcctccgata acactgtaaa aagataagag
     2821 gataaagagg gcaaaggcgc caatccaacc gataaagttg tacttgttat tcttgcccag
     2881 ttttccaaag gttttgatag cggaaacgcc agcactacgg ccaagggcaa actcagccag
     2941 caggagaggg aaaccgatta aaatagtgga aatgagaaag attagtaaaa agcctccacc
     3001 gccattagca gcagtcatgt agggaaactt ccaaacggaa ccaagcccga tgaccgagcc
     3061 agcagatgct agaataaagc caagtttcga cccccattgt gatttttctg acatagttaa
     3121 actctgatct cctttttcaa aataagaaaa agctacttga gttgtcaaat agccttctgt
     3181 ttgattgata gacttgacta tcctattatg ttttctaagg tctgtcaaga aaaccatttt
     3241 caagttttca cacctttctc aaaaacgctt gactctgacc taaggggagg ggttatacta
     3301 tcattgtaag gaggaaatca tgtaccatat aaaagaagct gcgcagcttt caggtgtctc
     3361 tgtcaagacc ctgccctgca tcactatgac aagataggac tcttggtccc cttaaagtcg
     3421 gaaaacggct atcgaaccta tagtcaagag gatttggaac gccttcaggt cattctttac
     3481 tacaaatatc taggcttttc tttagagaaa atagcagagc tgttaaagga agaaaggaca
     3541 gatttattgc cccatttaac taggcagttg gactatctaa ctcgcgaaag gcaacatctg
     3601 gataccttga tttccacctt gcaaaaaact attcaagaac aaaaaggaga aagaaaaatg
     3661 accattgagg aaaaattcac gggatttagc tatcaagaca atcaaaaata ccaccaagaa
     3721 gcggtagaga aatatggtca agaagtcatg ggacaagcgc ttgaacgcca aaaaggtcac
     3781 gaaaacgagg ctacggccgc ctttaaccaa gtctttcaaa ctttggcaca aaatcttcaa
     3841 gctggtttac ctgcaacagc aaccgaaaac caagagcaag cagccaagct cttgcaagcc
     3901 attcgcactt atggatttga ctgctctatt gaggtattcg gtcatatcgg taaaggttac
     3961 gtctacaacc cagagtttaa ggaaaacatt gacaagtttg gttctggaac agcccagtac
     4021 acgtcagatg ccattgcggc ttacgttcag acaaatgcag aaaaaatagg ctaggaattt
     4081 cctagcctat tttttacttc aaatcataaa gccagtcgtc accgtttttg tagtaaaaga
     4141 attcactgag atcttcttct agaaacacac gaagcatatc agacatatca tcggttgcaa
     4201 gttttagatg agaaagattt tcaaagtcct cccactaaac tttcccttcg tctgaagact
     4261 ggagttcacc agtaaagtgt tctgtcttgt aaaaaaggac gacataacga taatccttgt
     4321 cgtcatacca gtttttgata ccacagagtt ggggtttgga aatgatcaga ccagtttctt
     4381 ctttcacttc acgaatgaca gcatcgacaa aggattcgcc acgttcaaca tgaccaccag
     4441 gaaaagtaat gccagaccag tcgggactaa ctcggtcttg gaccaggacc ttatctccgt
     4501 ttttaatcat acacatgtta acaaattcga ctgcctctct tctgttcatt cttcacaacc
     4561 tttaatcttt aatcataatg cagacttccc gccacccagc cggtacagag ggcagaagtg
     4621 atgttaaagc cacccgtgtg ggcattgata tccataactt cgcctgcaaa gtggaggcca
     4681 ggtaccagct tactttcaag ggttttagga ttgatttcct tgagactgac tccacccttg
     4741 gtaacaaagg actttgcaag ggacattttt ccagttacag gaattttaag ttctttaatg
     4801 gactggacaa gttgttctcg ttccttttca gtcagctgtt tgactttttc aggatatcct
     4861 tgtacaaaaa attcggccaa gcgttctggt aacaaggttt ttaaagcgtt tttcaaggat
     4921 ttttcccgat tttcttctag aaatgtaacc aagtccttct cagaaagttg aggcaaaaca
     4981 tcgagtgaga gaacctcccc acctttgaca aagctagaca tgcgtagggc agcaggacct
     5041 gacaaaccaa agtgggtaaa gagtaaatca tgagtgatga catgcttacc ataacttagg
     5101 gtcacatcgt ccaaagaaat cccttgtaag gctttatgtg gaaaatctgt taataaagga
     5161 ctttcagcag cctcaagctc ggtgatggta tgcttaaaat ggcgagcaat ctcgtgacca
     5221 aaaccagtcg aaccagtcga aggataagac ttaccacctg ttgtgacaat gagtttctca
     5281 caagtgaagg tttgatccgc tgacttaagg acaaactggt catctacttt tttaacagaa
     5341 acgatttcta tttgagtagc aacttgacca cctagttcag tgattttctt ttccaaagct
     5401 tcgataatag tccgagactt gtcactggct ggaaagacgc gtccgtggtc ttcgacctta
     5461 agtttaacac cattttctgt aaaaaagttg atgatgtcat gattatcgaa ctgggagaaa
     5521 acactgtaaa gaaagcgtcc gtttccagga attccagcta gcaggttgtc taagctacca
     5581 ttgttggtca cattgcaacg tcccccacca gtcccagcta atttttttcc aagtttccga
     5641 tttttttcga tgaggagggt tttctgtcca taaaagctac tggaaatcgt agccatcata
     5701 ccagcaggtc ccccaccgat gacaatagta tcaaaatgtt tcatagctct attgtaccac
     5761 aaaaaaacaa gagatgatgg tcacctcttg tcaagaatgc aattaatcaa tttcatagcc
     5821 catcagcaaa ccgccctctt ctgcatagaa actgcagaga ccagaggttg gtagaatttt
     5881 aatatccgct tgtgggaagg tttcacggat tcgctctgag agctgttgac aacatttttc
     5941 gttattgcgt tgggccatga caatacggcc accagcatat ccagctttta ctaactcatc
     6001 ataggcagct tgaactgatt tctttgatcc ccttgctttt tgtagcaatt cgagagttcc
     6061 agtttcacta gcttttccga ccatacgaat gttgagaagg ccaacgaccg taccgataag
     6121 cttgctcaaa cggccgttct tcaccaagtt atcgactttg gctaggacaa agagcaactt
     6181 agttttttct tgataggcgg tgatagcttc aaccacttct tcaaaagaca agccctggtc
     6241 aatcaagtca ttcaattttt ctacgagtag gtcaacttca ccaccagcag ataaactatc
     6301 aatcacatga atcttagtgt caggatggtc ttccagataa atattctttg ctagttgagc
     6361 actattgtga ctgccagaaa gggtacctgt gatggttact aggaaaatgt ttttggcacc
     6421 ttcaaatgct cgcaaatagt catctgggct tggacaagcc gattttgaag cttctgcagt
     6481 tgcatacatg gtttccatca tttggtcaat atcgagactg gcgtcatcaa caaagacctg
     6541 atcagctact tgaatggtta aggggacact tacaaaggtt gtgttaatag ctggtgttgg
     6601 cagttgacga taatcacaac cagagtcagc aataatcttc caagtcatag aaattctcca
     6661 tctttgtcag ttatacattg acaaaggttc tgtctttttt tacaattata tcatgaaaac
     6721 ccttgaaaca aaagcatcat ccgtctgttg tgacaagtag aaagaaaatg ttatgtctga
     6781 acgtagaatc tctgaaaagt ctcttgaaaa tctcagaaaa tcaaaccaag aatccaattt
     6841 attaaccaga gaagccattg aaacagccct cttgcaactc ttggaaaaaa aggagctgac
     6901 caagattagt atttctgaat tggtcaaacg tgcaggtgtt tcgcgtgcgg ccttttatcg
     6961 caattatgac tccaaagagg aaattttaga gagcgtcttt aaacgaactg tccacaatat
     7021 tatggaacag ctgcatcatt acgatttaaa gacagacctt tatttggttt gggttcacct
     7081 tttccgggag gccagaaagg aagctagagt gattcaattg gccttggatt accatctgga
     7141 aaaaatcttt gtccaagcca tgcaggaatt tctagaaaaa taccatggga aatcaaaagg
     7201 tgtcagctct tatcttcatt ccttctggag ctcagccatc gtctctgtcc ttctaaaatg
     7261 gatcaaggat ggcatgaagg taccagctga aaagattgca gatttaggtt taccattttt
     7321 taaaaaatag agaaaaagga gaaaagagat gactgaaaaa agactggcct gggatgagta
     7381 ttttgcagcc caagctctat taattgcgaa tcgttccact tgtaaacgtg ctaaagtggg
     7441 cgcgattctg gtaaaagata ataaggttat ttccactggt tacaatggtt cggtgtcagg
     7501 gactgagcat tgtattgatc acgaatgttt ggtcattgaa ggccactgtg ttcgcaccct
     7561 tcatgctgag gtcaatgcta tccttcaagg tgctgagcgt ggtgttccca aaggctttac
     7621 agcctatgta acccattttc cttgtctcaa ctgtacaaaa caattgcttc aggtcggctg
     7681 caagcgcgtg gtttatatca accagtaccg aatggacgat tacgcccaat acctttatca
     7741 agaaaaaggg acagaattga cccatttacc acttgagaca gtacagacag ctcttaaaga
     7801 ggctgatcta atgtaaaaat tatcaaaaat aaatggttta gaaagatttt taaaccgttt
     7861 tttggtataa taagaagaat aaattgaaag aaggaattcc aaaaatggga aaaattgaag
     7921 ttattaatca cccactgatt caacacaaat tgtcaatctt gcgtcgtaca gatacttcta
     7981 caaaagcttt tcgtgagcta gtagatgaga ttgcaatgtt gatggggtat gaagtacttc
     8041 gtgatcttcc actagaagat gtggaaatcg aaacaccaat tacaaaaaca gttcaaaaac
     8101 aattggcagg taagaaattg gccatcgtcc caatcttgcg tgcaggtatc gggatggttg
     8161 atggtctctt gaacttggtt ccagctgcta aagttggcca catcggtatg taccgtgatg
     8221 aagaaacact tcaaccagtt gaatacttgg tgaaattgcc tgaggacatt gaccaacgtc
     8281 aaatttttgt agtagaccca atgttggcaa caggtggctc agcaatcttg gctgttgatt
     8341 ctcttaaaaa acgtggcgca tcaaatatca aatttgtctg ccttgtatct gctccagagg
     8401 gtgtaaaagc ccttcaagaa gctcatccag atgtagaaat ctttacagca gccttggatg
     8461 aacgtttgaa cgaacacggt tatatcgttc caggtcttgg agatgctgga gaccgcttgt
     8521 tcggtacaaa ataagatcga aaagaagaat gacttggaaa agtatttcca gtcacgaaag
     8581 gaggttgggt ttttgtttct gtctaatgaa agcagagcaa aaatttgacc ttttttgacc
     8641 aagatattat aatagtctta tcttcagtca tttgaccaac aaaattaaaa ctcaaaagga
     8701 gaaatgaatg attcctgtag ttattgaaca aacaagccgt ggagaacgtt cttacgatat
     8761 ttactcacgt cttctcaaag accgcatcat tatgctgaca ggtccggttg aagacaatat
     8821 ggctaactct gttattgccc aactgctttt cttggatgcc caagatagta caaaagatat
     8881 ttacctttat gtcaatacac caggtggttc tgtttcagct ggtttggcaa tcgtagatac
     8941 catgaacttt atcaaggcag atgtccaaac cattgttatg ggaatggctg catctatggg
     9001 aacagtcatc gcatcaagtg gagcaaaagg caaacgtttc atgcttccaa atgctgaata
     9061 catgattcac caaccaatgg gcggtacagg tggtggtacc caacaaactg atatggctat
     9121 cgctgcagaa cacttgctca aaactcgtaa taccttggaa aaaatcttgg ctgaaaattc
     9181 aggtcagtca atggaaaaag tccatgcaga tgcagaacgt gataactgga tgagcgccca
     9241 ggaaacactt gaatatggct ttattgatga aattatggcc aacaattcat tgaactaatg
     9301 atgatagaag gcaaactcga ctgggtttgc tttttttggt ataataggga gagatttctt
     9361 agaaagagga tttatcatgt ttgaaaaagt caatcgctct ggcttgatta tctatcttta
     9421 ctataatcgt gatgccaaaa aactgcagga ttatggagat attacctatc attccaagaa
     9481 acatcgttac ttacaactct atgttccaac tcaagaagtg gagcaattgg tcggacgctt
     9541 gagcaaggaa aaatttatta aaaaagttag ggtttgtcat atccaagagt tggaaacacc
     9601 ctttgtgggc aatctttatc gataggaaaa cgttatcatc gaaaaagtcc aaggaaaaca
     9661 ttgacaattt tctgataatt cggtatattc ttaacagact atttaagaaa taaggagaca
     9721 aaaaagatga agaaaaaatt tgccctatcg tttgtggcgc ttgcaagtgt agcacttctt
     9781 gcagcctgtg gagaagtgaa gtctggagca gtcaacactg ctggtaactc agtagaggaa
     9841 aagacaatta aaatcgggtt taactttgaa gaatcaggtt ctttagctgc atacggaaca
     9901 gctgaacaaa aaggtgccca attggctgtt gatgaaatca atgccgcagg tggtatcgat
     9961 ggaaaacaaa tcgaagtaat cgataaagat aataagtctg aaacagctga ggctgcttca
    10021 gttacaacta accttgtaac ccaatctaaa gtatcagcag tcgtaggacc tgcgacatct
    10081 ggtgcgactg cagctgcggt agcgaacgct acaaaagcag gtgttccatt gatctcacca
    10141 agtgcgactc aagatggatt gactaaaggt caagattacc tctttattgg aactttccaa
    10201 gatagcttcc aaggaaaaat tatctcaaac tatgtttctg aaaaattaaa tgctaagaaa
    10261 gttgttcttt acactgacaa tgccagtgac tatgctaaag ggattgccaa atctttccgc
    10321 gagtcataca agggtgaaat cgttgcagat gaaactttcg tagcaggtga cacagacttc
    10381 caagcagccc ttacaaaaat gaaagggaaa gactttgatg ctatcgttgt tcctggttac
    10441 tataatgagg ctggtaaaat tgtaaaccaa gcgcgtggca tgggaattga caaaccaatc
    10501 gttggtggtg atggattcaa cggtgaggag tttgtacaac aagcaactgc tgaaaaagca
    10561 tcaaacatct actttatctc aggcttctca actactgtag aagtttcagc taaagctaaa
    10621 gccttccttg acgcttaccg tgctaagtac aatgaagagc cttcaacatt tgcagccttg
    10681 gcttatgatt cagttcacct tgtagcaaac gcagcaaaag gtgctaaaaa ttcaggtgaa
    10741 atcaaggata accttgctaa aacaaaagat tttgaaggtg taactggtca aacaagcttc
    10801 gatgcagacc acaacacagt caaaactgct tacatgatga ccatgaacaa tggtaaagtt
    10861 gaagcagcag aagttgtaaa accataatag aaaaatgttg aaatagggaa tgagcctttg
    10921 actcactccc tgtttcgata tttaatactc ttcgaaaatc tcttcaaact gcgtcaacgt
    10981 cgccttggat tatatatgtg actgacttcg tcagtcttat ctacaacctc aaagcagtgc
    11041 tttgagcaac ctgcggctag tttcctagtt tgctctttga ttttcattga gtataagaac
    11101 ctatcaaaaa gtgagggaaa cccctcggaa ttataaatag aaagagtgaa tcttatgctc
    11161 caacaactcg taaatggttt gattctaggt agtgtttacg cgctgttagc cctaggatat
    11221 accatggttt acggaattat caagctcatc aacttcgccc atggtgatat ttatatgatg
    11281 ggagccttta tcggttattt cttgatcaat tctttccaaa tgaatttctt tgtagcgctt
    11341 attgtagcta tgctagcgac agctattctt ggtgtcgtga ttgagtttct tgcttaccga
    11401 cctttgcgcc actctactcg tattgctgtt ttgattacgg ctattggggt ttctttccta
    11461 ttggagtatg gaatggtcta tctggttggt gccaataccc gtgccttccc tcaagcgatt
    11521 caaacagttc gatatgattt gggaccaatt agcttaacaa atgtgcagtt aatgattttg
    11581 gctatttcct tgattttgat gattttgtta caagtcattg tccaaaagac taagatgggg
    11641 aaagccatgc gtgcagtatc agtagatagc gacgcggcgc aattgatggg gatcaatgta
    11701 aaccgtacga ttagctttac cttcgctttg ggttctgctc ttgcgggtgc ggctggtgtt
    11761 ctgattgctc tttattataa ctctcttgag cctttgatgg gggttactcc aggtcttaaa
    11821 tctttcgttg ccgcagtact tggtggtatc ggaattattc ctggtgcggc tcttggtggc
    11881 tttgtgattg gtctattgga aacctttgcg actgcctttg ggatgtcaga tttccgtgat
    11941 gccattgttt atggaatctt gttgttgatc ttgattgtcc gcccagctgg tatccttggt
    12001 aagaatgtga aagagaaggt gtaaacgatg aaggaaaatt taaaagttaa tattctatgg
    12061 ttactccttt tgttagctgg ctatagcttg attagtgtac tggtttcagt cggagtactt
    12121 aatctattct atgtacagat tttacaacaa attggaatta atattatttt ggctgttggt
    12181 ctcaacttaa tcgttggttt ttcaggacaa ttttcacttg gtcatgctgg tttcatggcg
    12241 attggtgcct atgcagcagc tattattggt tctaaatcac caacctacgg tgccttcttt
    12301 ggagctatgc ttgtaggggc tttgctttca ggagcagttg ccttacttgt cggcattcca
    12361 accttgcgct tgaaggggga ctatcttgcg gtagcaactc taggtgtttc tgaaattatc
    12421 cgtatcttta tcatcaatgg tggaagcctt acaaatggtg cggcaggtat cttagggatt
    12481 cctaacttta caacttggca aatggtttac ttctttgtcg tgattacaac cattgcaacc
    12541 ttgaacttct tgcgtagccc aattggtcgt tcaaccctct ctgttcgtga agatgaaatc
    12601 gctgctgagt cagttggggt taatacgact aaaattaaaa tcatcgcttt tgtctttggt
    12661 gccattactg caagtattgc tgggtcactt caggcaggat ttatcgggtc tgttgtaccg
    12721 aaagattaca ccttcatcaa ctcaatcaac gttttgatta ttgttgtatt tggtggactc
    12781 ggttccatta caggtgcgat tgtttcggct attgttctgg gaattttgaa tatgcttctc
    12841 caagatgttg ctagtgtgcg tatgattatt tacgctttgg ccttggtatt ggtaatgatt
    12901 ttcagaccag gtggactcct tggaacgtgg gaactgagcc tatcacgttt ctttaaaaaa
    12961 tctaagaagg aggaacaaaa ctaatggcat tacttgaagt aaaacagtta accaaacatt
    13021 ttggtggtct aacagctgtt ggagatgtga ctcttgaatt gaacgaaggg gaactggttg
    13081 gattaatcgg tccaaacgga gctgggaaaa ccaccctttt caaccttttg accggtgttt
    13141 atgaaccaag cgagggaaca gtaaccctag atggtcacct tttgaatggg aaatcacctt
    13201 ataagattgc ctctttggga cttggacgta ctttccaaaa tatccgtctt tttaaagatt
    13261 taacagtttt agacaatgtt ttgattgctt ttggaaacca tcacaaacag catgttttta
    13321 ctagtttctt acgcttacca gctttttaca agagtgaaaa agaattaaag gctaaagctt
    13381 tggaattgtt gaaaatcttt gatttagatg gtgatgcaga gactcttgct aaaaatcttt
    13441 cctacggaca acaacgtcgt ttggaaattg ttcgtgccct tgctacggaa cctaaaattc
    13501 tcttcttaga tgaaccagca gcaggtatga acccacagga aacagccgaa ttgactgagt
    13561 taattcgtcg tatcaaagat gaatttaaga ttacaatcat gttgattgaa cacgatatga
    13621 atctggtcat ggaagtaaca gaacgtatct acgtacttga atatggccgt ttaattgctc
    13681 aaggaactcc agacgaaatt aagaccaata aacgcgttat cgaagcttat ctaggaggtg
    13741 aagcctaatg tctatgttaa aagttgaaaa tctttctgtg cattacggta tgatccaagc
    13801 agtccgtgat gtaagctttg aagttaatga aggagaagtt gtttccctta tcggtgccaa
    13861 cggtgcaggt aagacaacta ttcttcgcac cttgtcaggt ttggttcgac caagttcagg
    13921 aaagattgaa tttttaggtc aagaaatcca aaaaatgcca gctcagaaaa ttgtggcagg
    13981 tggtctttca caagttccag aaggacgcca cgtctttcct ggcttgactg ttatggaaaa
    14041 tcttgaaatg ggagctttct taaagaaaaa tcgtgaagaa aatcaagcta acttgaagaa
    14101 ggttttctca cgctttcctc gtcttgaaga acgtaagaac caagatgcag ctactctttc
    14161 aggaggggaa caacaaatgc ttgccatggg acgcgctctt atgtcaacac caaaacttct
    14221 tcttttagat gaaccatcaa tgggacttgc cccaatcttc atccaagaga tttttgatat
    14281 cattcaagat attcagaagc aaggaacaac cgtcctcttg attgaacaaa atgccaataa
    14341 agcacttgca atctctaacc gaggatatgt actggaaaca gggaaaatcg tcctatcagg
    14401 aacaggaaaa gaactcgcct catcagaaga agtcagaaaa gcatatctag gtggctaaaa
    14461 caatccagtg gattgtttta gtcggcagat gaagattacg aagtaatcat cattatagtc
    14521 tgggagacta gtttaggttg cagataaaga ttgcgaagca atcatcaaat ccagaggatt
    14581 gttttagccg gcagatggag attacgaagt aatcatcaat atagtccggg ggaccttttt
    14641 agtcggtgga tagagattgc aaacaaatat gcatctacat tgaaagctta atttctaata
    14701 attgaaaaaa tcgaatgaaa aatttcttac cttcattcac agagctctat ttcagagctc
    14761 tttttgctag cttattcata cttttttgaa tttcgaaaaa gaaatgtaag cgtttgatag
    14821 atttacaaaa agattgtata atggggataa gaatagaaaa ggagaagtct catggcagtt
    14881 aaagatttta tgacccgcaa ggtagtttat attagtccag atacaacagt atctcatgca
    14941 gcagatttga tgagagagca aggtttgcac cgtctgcctg ttatcgaaaa tgatcaatta
    15001 gttggtttgg tgactgaggg aaccattgca caagcaagtc catctaaagc aacaagtctt
    15061 tctatctatg agatgaatta tcttctgaat aagacaaaag taaaagatgt catgattcgc
    15121 gatgttgtca ctgtctcagg ctatgctagt ctagaagatg caacttatct gatgttgaaa
    15181 aataagattg gtattctccc tgtcgtagat aactatcaag tatacggagt tattactgac
    15241 cgtgatgttt tccaagcctt tcttgaaatt gcaggttatg gcgaagaagg gattcgtgta
    15301 cgctttgtta cagaagatga agttggtgtt cttggaaaaa ttgtttcttt gattgtagaa
    15361 gaaaatttga atatctccca tacagtcaat attccgcgta aggatggtaa ggtgattatc
    15421 gaagtgcaaa tcgatgaatc aattgattta ccagccttga aagaaaaatt tgaagcaaat
    15481 ggtattcaag tggaagaaat cgctcgcact tcagcaaaag tcttgtaaga agggaagtcc
    15541 aaaggcttct tttttcatga aaaggggatt agagcaaaag atggaaagaa atgataaaat
    15601 atgctataat gaaataatat aaaaaaggag tatttatgga catttcagta attcgtcaga
    15661 aaattgacgc aaatcgtgaa aaattagctt ctttcagggg gtctctttga cctcgaaggg
    15721 ctagaggaag agattgccat cttggaaaac aagatgacag aacctgattt ttggaacgat
    15781 aatattgcgg cccaaaaaac gtcgcaagaa ttgaatgaat taaaaaacac ttacaatacc
    15841 ttccataaga tggaagagtt gcaggatgaa gtcgaaattt tattggattt tttggctgaa
    15901 gacgagtcag tgcatgatga actggtagcg cagttagccg aacttgataa gataatgacc
    15961 agctacgaga tgactctact cttgtcagaa ccttatgacc acaacaatgc catcttggaa
    16021 atccatccag gttctggtgg tactgaggcg caggactggg gtgatatgtt gcttcgtatg
    16081 tatactcgtt atggtaatgc taaaggcttt aaagtggaag tgttggatta ccaagcaggt
    16141 gatgaggctg gtattaagtc ggtaacttta tcatttgaag ggcctaatgc ctatggtctc
    16201 ctcaagtcag aaatgggtgt tcaccgctta gtgcgaatct caccatttga ctctgccaaa
    16261 cgtcgccata cctctttcac atctgtagaa gtgatgccag aattggatga tactattgaa
    16321 gtggaaatcc gtgaagatga tatcaagatg gataccttcc gttcaggtgg tgccggtgga
    16381 caaaacgtca ataaggtttc aacaggtgta cgtttaaccc acattccaac tggaattgtt
    16441 gtccaatcaa cagtggatcg tacccagtat ggaaatagag atcgtgccat gaagatgttg
    16501 caggctaagc tctatcaaat ggagcaagat aagaaggctg cggaggtaga ttctctcaaa
    16561 ggtgagaaaa aggagatcac ttggggaagc caaatccgtt cttatgtctt cacgccttat
    16621 actatggtaa aagatcaccg aactagcttt gaggttgctc aggtagataa ggttatggat
    16681 ggggacctag atggttttat cgatgcttat ctcaagtggc gaattagcta agatagaaag
    16741 gaactcacat gtcaattatt gaaatgagag atgtcgttaa aaaatacgac aacggaacga
    16801 ctgctctacg cggtgtttcg gtcagcgttc aaccggggga atttgcttac atcgtaggac
    16861 cttcaggagc agggaagtca acttttattc gttctctgta tcgtgaagta aaaatcgata
    16921 aaggaagtct atcagttgct ggttttaatc tggttaagat caaaaagaaa gatgtcccgc
    16981 ttctacgtcg tagtgttggg gttgtcttcc aagattataa attgttacca aagaaaactg
    17041 tctatgaaaa tattgcttac gctatggaag taatcgggga aaatcgccgt aatatcaaaa
    17101 gacgagtgat ggaagttttg gacttggttg gattgaagca taaggttcgt tctttcccaa
    17161 atgaactctc aggtggggag caacagcgaa ttgcgattgc gcgtgcaatt gtaaataatc
    17221 ccaaagtatt gatagctgat gagccaacag gaaatctgga tccggataat tcatgggaaa
    17281 ttatgaatct cttggaacgg attaacctac aagggacaac tattttgatg gcgactcata
    17341 atagccagat tgtaaatacc ttgcgccacc gtgtcattgc cattgaaaat ggccgtgtcg
    17401 ttcgtgacga atcaaaagga gagtatggat acgatgatta gtagattttt tcgccattta
    17461 tttgaagcct taaaaagttt gaaacgaaat ggttggatga cagtagctgc tgtcagttca
    17521 gtcatgatta ctttgacctt ggtggcaata tttgcatctg ttattttcaa tacagcgaaa
    17581 ctagctacag atattgaaaa taatgtccgt gtagtagttt atatccgaaa ggatgtggaa
    17641 gataatagtc agacaattga aaaagaaggt caaactgtta caaataatga ctaccacaag
    17701 gtatatgatt ctttgaagaa catgtctacg gttaaaagtg ttaccttttc aagtaaagaa
    17761 gaacaatatg aaaaattaac cgagataatg ggagataact ggaaaatctt tgaaggagat
    17821 gccaatcctc tctatgatgc ctatattgta gaggcaaaca ctccaaatga tgtaaaaact
    17881 atagccgaag atgctaaaaa aattgaaggt gtctctgagg ttcaagatgg cggtgccaat
    17941 acagaaagac ttttcaagtt agcttcattt atccgtgttt ggggactagg gattgctgct
    18001 ttgttaattt ttatcgcagt tttcttgatt tcaaatacca ttcgtattac cattatttcc
    18061 cgcagtcgcg aaattcaaat catgcgcttg gtcggagcta aaaacagtta tatccgtgga
    18121 ccgttcttgt tagaaggagc ctttatcggt ttattgggag ctatcgcacc atctgttttg
    18181 gtctttattg tttatcaaat tgtttaccaa tctgtcaaca aatcgttggt agggcaaaat
    18241 ctatccatga ttagtccaga tttatttagt ccgttgatga ttgccctact atttgtgatt
    18301 ggggttttca ttggttcatt gggatcagga atatccatgc gccgattctt gaggatttag
    18361 gtaaaatagc tgcttttatg aggagattgt aaaatctcct tttttgctac aagagttttt
    18421 gaaaagagat gcgtagaaga aaagagcttc caaagaagtc ccccagagaa gacttcttga
    18481 tggctaattt gaacttgaaa tttggcgcac tagaaatttt ttagaaaaaa gtgtaatttt
    18541 tttataaaag cctttacaaa aaaaattaaa gtggtataat taaatcatag aaggtgcaaa
    18601 cgttttcgta aaacgtttgc ctaaataaaa ataaaggaga tttgaatgat gaaagataca
    18661 ttcaaaaatg tcttgtcttt cgaattttgg caaaaattcg gtaaggcttt gatggtagtt
    18721 atcgcggtta tgccggctgc tggtttgatg atttcaatcg gtaagtctat cgtgatgatt
    18781 aacccaacct ttacaccact tgtcatcaca ggtggaattc ttgagcaaat cggttggggg
    18841 gttatcggta accttcacat tttgtttgcc ctagccattg gaggaagctg ggctaaagaa
    18901 cgtgctggtg gtgctttcgc cgctggtctt gccttcatct tgattaaccg tatcactggt
    18961 acaatttttg gtgtatcagg cgatatgttg aaaaatccag atgctatggt aactactttc
    19021 tttggtggtt caatcaaagt tgctgattac tttatcagtg ttcttgaagc tccagccttg
    19081 aacatggggg tattcgtagg gattatctca ggttttgtag gggcaactgc ttacaacaaa
    19141 tactacaact tccgtaaact tcctgatgca ctttcattct tcaacgggaa acgtttcgta
    19201 ccatttgtag ttattcttcg ttcagcaatc gctgcaattc tacttgctgc tttctggcca
    19261 gtagttcaaa caggtatcaa taacttcggt atctggattg ccaactcaca agaaactgct
    19321 ccaattcttg caccattctt gtatggtact ttggaacgtt tgctcttgcc atttggtctt
    19381 caccacatgt tgactatccc aatgaactac acagctcttg gtggtactta tgacatttta
    19441 actggtgcag ctaaaggtac tcaagtattc ggtcaagacc cactatggct tgcatgggta
    19501 acagaccttg taaaccttaa aggtactgat gctagtcaat accaacactt gttagataca
    19561 gtacatccag ctcgtttcaa agttggacaa atgatcggtt cattcggtat cttgatgggt
    19621 gtgattgttg ctatctaccg taatgttgat gctgacaaga aacataaata caaaggtatg
    19681 atgattgcaa cagctcttgc aacattcttg acaggggtta ctgaaccaat cgaatacatg
    19741 ttcatgttca tcgcaacacc tatgtatctt gtttactcac ttgttcaagg tgctgccttc
    19801 gctatggctg acgtcgtaaa cttacgtatg cactcattcg gttcaatcga gttcttgact
    19861 cgtacaccta ttgcaatcag tgctggtatt ggtatggata tcgttaactt cgtttgggta
    19921 actgttctct ttgctgtaat catgtacttt atcgcaaact tcatgattca aaaattcaac
    19981 tacgcaactc cagggcgcaa cggaaactac gaaactgctg aaggttcaga agaaaccagc
    20041 agcgaagtga aagttgcagc aggctctcaa gctgtaaaca ttatcaacct tcttggtgga
    20101 cgtgtaaaca tcgttgatgt tgatgcatgt atgactcgtc ttcgtgtaac tgttaaagat
    20161 gcagataaag taggaaatgc agagcaatgg aaagcagaag gagctatggg tcttgtgatg
    20221 aaaggacaag gggttcaagc tatctacggt ccaaaagctg acattttgaa atctgatatc
    20281 caagatatcc ttgattcagg tgaaatcatt cctgaaactc ttccaagcca aatgactgaa
    20341 gcacaacaaa acactgttca cttcaaagat cttactgagg aagtttactc agtagcagac
    20401 ggtcaagttg ttgctttgga acaagtaaag gatccagtat ttgctcaaaa aatgatgggt
    20461 gatggatttg cagtagaacc tgcaaatgga aacattgtat ctccagtttc aggtactgta
    20521 tcaagcatct tcccaacaaa acatgctttt ggtattgtga cggaagcagg tcttgaagta
    20581 ttggttcaca ttggtttgga cacagtaagt cttgaaggta aaccatttac agttcatgtt
    20641 gctgaaggac aaaaagttgc agcaggagat ctccttgtca cagctgactt gaatgctatc
    20701 cgtgcagcag gacgtgaaac ttcaacagta gttgtcttca caaatggtga tgcaattaaa
    20761 tcagttaaat tagaaaaaac aggttctctt gcagctaaaa cagcagttgc taaagtagaa
    20821 ttgtaatata cttgaggttg gaagctgtat tccaacctct tattttggga gaaaagaatg
    20881 aaatttttaa cactcaatac tcacagttgg atggagaaag aagcagagga aaaattccag
    20941 attttgcttg aagatattct tgaaaaggac tatgatttga tttgtttcca agaaatcaat
    21001 caggagatga cctcgtcaga ggtggaggtt aatgaccttt atcaagcttt gccagcagct
    21061 gagcctattc accaagacca ttatgttaga ctcttggttg aaaagttgtt agagcaaggg
    21121 aaaaattact actggacctg ggcttataac catatcggct atgaccgcta ccacgaaggt
    21181 gtggctatct tgtctaaaac acctattgaa gccagagaaa ttttggtttc agatgtggat
    21241 gatccaacag actatcatac tcgccgtgtt gccctagctg aaactgtagt cgatggcaag
    21301 gagctagcag ttgccagtgt tcatctctct tggtgggata aaggtttcca agaagaatgg
    21361 gcacgatttg aggctgtctt gaaaaaattg aacaagccac ttttactagc tggagatttt
    21421 aacaacccag caggtcagga aggttaccaa gctattttag ctagtccatt aggcttacaa
    21481 gacgcatttg aagttgctca agagaaaagt ggtagctata ctgttccgcc tgaaattgat
    21541 ggctggaaag ggaacactga accccttcga atcgattatg tctttactac caaagagtta
    21601 gcggtggaaa atttacatgt cgtatttgat ggtaacaaga gtccacaagt gagtgatcac
    21661 tatggcttga atgctatgtt aaactggaaa taataactga aaagaggttg gaactataaa
    21721 attccagcct tttcttacta gagaagctac tggaaatagc ctaaataagt gagactactg
    21781 taatggaata aaatatggta taattgataa gatagataga atcgaggatg ttatgtcatt
    21841 tacgaaattt caatttaaaa actatattag agaagccttg aaggagttaa aatttacaac
    21901 tccaacagag gtgcaagaca agttgattcc tattgttttg gcaggtcgtg acctagtagg
    21961 agaatcaaaa acaggttcag gtaagactca tactttcttg ttaccgattt ttcagcaatt
    22021 agatgaagct agcgatagtg tacaagcagt gattactgca ccgagtcgtg agttggctac
    22081 tcaaatttac caagtagcgc gtcagatttc agctcactca gatgtcgaag ttcgtgtggt
    22141 taattatgtg ggtggtacgg ataaggctcg ccagattgag aaattggcaa gcaatcagcc
    22201 tcatattgtt attggaacac caggccgtat ctacgacttg gttaaatctg gtgatttagc
    22261 tattcataaa gccaagacat ttgttgttga tgaagcggat atgaccttgg atatgggatt
    22321 cttggaaact gttgataaga ttgctggcag tcttccaaaa gacttgcaat tcatggtctt
    22381 ctcagcgact atcccacaaa aactgcaacc attcttgaaa aaatacttat ccaatcctgt
    22441 tatggagaaa attaagacca aaacggttat ttctgacacc attgataatt ggttgatttc
    22501 gaccaaggga catgataaga atgctcaaat ttaccagttg actcagttga tgcagccgta
    22561 tttggcaatg atttttgtta acactaaaac gcgtgctgat gaattgcatt catatctgac
    22621 tgctcaaggc ttgaaggttg caaaaatcca tggcgatatt gcccctcgtg aacgcaagcg
    22681 aatcatgaat caggtgcaaa atctggattt tgagtatatt gtcgcaacag atttagcagc
    22741 gcgtgggatt gacattgaag gtgtcagcca tgtcatcaat gatgccattc cgcaagactt
    22801 atcttttttt gttcaccgtg ttggtcgtac tggacgaaat ggcctaccag gtacagctat
    22861 taccctttat cagccaagtg atgactcgga tatccgtgag ttggagaaat tgggaatcaa
    22921 gtttagtcct aagatagtca aagatgggga atttcaagat acctatgacc gtgatcgtcg
    22981 tgccaaccgt gagaaaaaac aagataaact tgatatcgaa atgattggtt tggttaaaaa
    23041 gaaaaagaaa aaagtcaaac cgggttataa gaagaaaatt caatgggcgg ttgatgaaaa
    23101 gcgccgtaaa accaagcgtg ctgaaaatcg cgctcgcggt cgtgcagagc gtaaagctaa
    23161 acgccaaaca ttttaataga aattgttgga gtactgagct ccaacttttt tatttatgag
    23221 aacgaactat ctaaaccgaa acactacatt aaagactgca aattgcgatt aaaaatggta
    23281 taatgataaa gttatatagt cccgataaga tggtaggtat ttattacgaa gagttttcct
    23341 atcagtagtt tgcaattcgc aaagaatgac ggcttcgagg tcgctcgtct ttctacggtt
    23401 gctgccatac tgcggtcacc ctatgcgcct tactagcttc agaaataaaa aagtatcgtt
    23461 tttatttctg aagctagtaa ctcttagata atggaaattc ctatcagtac tttgtaactc
    23521 tataacaata ttttttaagg ggggacattt ttatgtcaga acgtaaatta ttcacgtctg
    23581 aatctgtatc tgaggggcat ccggataaga ttgcagacca aatttcagat gcgattttgg
    23641 atgctatttt agcaaaggat ccagaggcgc acgttgctgc tgaaacagct gtatatactg
    23701 gttctgtcca cgtttttggt gaaatttcta caaatgccta tgtggatatt aaccgtgtgg
    23761 ttcgtgatac cattgcagag attggttata ccaatacaga atatggattt tctgctgaga
    23821 cggtgggagt acacccatct ttggtggaac aatctcctga catcgctcaa ggtgttaacg
    23881 aagccttgga ggttcgtgga aatgctgatc aagatccact ggacttgatt ggagcaggtg
    23941 accaagggct catgtttgga tttgcagtag atgaaacaga agagctcatg ccattgccaa
    24001 ttgcactcag tcataaattg gttcgtcgtc tggcagaact tcgtaagtct ggagaaatta
    24061 gctatctccg tccagatgca aaatcacaag ttacagttga gtacgatgaa aatgaccgtc
    24121 cggtacgtgt agatacagtc gttatttcta ctcagcatga tccagaggcc actaatgaac
    24181 aaatccatca agatgtgatt gacaaggtca tcaaagaagt tattccatct tcttatcttg
    24241 atgataagac aaaattcttt atcaatccga caggtcgttt tgtaatcggt ggtcctcaag
    24301 gggactcagg tttgactggt cgtaagatta ttgtagatac ttatggtggc tactctcgtc
    24361 atggtggtgg tgccttctct ggtaaagatg cgactaaggt ggatcgttca gcctcttatg
    24421 cggctcgcta tattgccaag aatatcgttg cagcagacct tgctaagaag gcagaagtgc
    24481 agttggccta tgctatcggt gttgcgcaac ctgtttctgt tcgtatcgat actttcggta
    24541 caggaacagt agctgaaagt caacttgaaa aagcggctcg tcaaatcttt gaccttcgcc
    24601 ctgcagggat tatccaaatg ctggacctta agcgtccaat ttaccgtcaa acatcggctt
    24661 acggtcacat gggacgtaca gatattgatc ttccatggga acgtttggat aaggtagatg
    24721 ctttgaaaga agcagtaaaa taagatttta agaggggaac gtcctctctt ttttatagtt
    24781 tttaactgta ctgggatact gttctgaaaa tccattttga gaaagtagag atttacatgt
    24841 atagtagatt gaaactagaa tagtacacct caacttctaa aacattgtta gaaatcgatt
    24901 tgactgtcct gatcgatttc tcctgttctt gtttcatttt actatatttc tttaaaaatg
    24961 ataaaggttg agatttctcc tcgtaataga taatcatggg gatatttcaa gccaaagttt
    25021 tattcgttat cacttgacta ttgcaaggtt ttctagagca acagagtcat ggaatggact
    25081 catggttgag atttctcctt gttgcttgga cttcattcaa aagtctgtta cccaagcctt
    25141 gttcaaactt ccaatacact agctgtttcc atagcatgac ttctgtacta gactttcttt
    25201 tccgaataaa tagatagaac cacagaatct agtaaaccta gaattaaaat tatggtataa
    25261 tattagcaat aaaagaaatc tggaggatta gaatcatggt atcaacgaaa acacaaattg
    25321 ctggttttga gtttgacaat tgcttgatga atgcagcagg tgtggcttgt atgacgatag
    25381 aggagttaga agaggtcaaa aactcagcgg caggaacctt tgttactaag acagcgacct
    25441 tggacttccg tcaggggaat cctgagccac gctaccaaga tgttccactt ggttccatca
    25501 actctatggg cttgccaaat aatggcttag actattattt ggattatctt ttagatttgc
    25561 aggaaaaaga gtcgaaccga actttcttct tatctctggt cggcatgtct ccagaggaaa
    25621 cccatactat tttgaaaaaa gtccaagaga gtgattttcg tggtctgact gagctaaatc
    25681 tttcctgtcc aaatgttcca ggtaaacctc agattgccta tgattttgag acaacagacc
    25741 ggattttggc agaagtgttt gcttacttca ccaaacctct tggaattaaa ttgccacctt
    25801 attttgatat tgttcacttt gatcaagcgg cagctatttt caacaaatat ccgctcaagt
    25861 ttgtcaactg cgttaactct atcggaaacg gcctctatat agaagacgaa tctgtcgtta
    25921 ttcggcctaa gaatggtttt ggtggaattg gtggagaata catcaaaccg actgctctag
    25981 ccaatgttca cgccttttat caacgtttaa atcctcaaat ccaaattatc ggaacaggtg
    26041 gcgttctgac tggtcgagat gcctttgaac acatcctctg tggagcaagt atggtgcagg
    26101 tgggaacgac ccttcacaaa gaaggcgtca gtgcttttga ccgcattacc aatgaattga
    26161 aagcaatcat ggtggaaaaa ggctacgaga gcttagaaga tttccgtggg aaattgcgct
    26221 atattgacta aattaaattg aaaaatctga agaaaggaga gacgatgcta gccattgaag
    26281 aaagtcagaa gttgacttta tcaaatttac cgagcctgag cctatttaca gggacagatc
    26341 agggtcagtt tgaagtgatg aagagtcaag tgttgaaaca gattgggtat gattctgctg
    26401 acctcaactt tgcctacttt gatatgaaag aagtagttta caaggatgtg gaattggagt
    26461 tggtcagcct tcctttcttt gtggatgaga aaatcgtgat attagattat tttatggata
    26521 tcacgactgc taagaaacgc tttttgacag atgatgagct taagttattt gaggaatacc
    26581 ttgacaatcc ttctccaaca accaagttga taatctttgc agaaggaaag ctggatagca
    26641 aaagacggtt agtcaaatta cttaagcgtg atgccaaggt cttcgatgca gtagaagtaa
    26701 aagaacaaga attgcgccag tatttccaaa agtggagtca gaaacaaggt ctgcagttta
    26761 ccaattattc ttttgaaaat ctcctcatca agtcggggtt tcaatttagc gaaatccaga
    26821 aaaatcttct ctttttacag tcctataagg cggattctgt tatcgaggaa gaggatattg
    26881 ttaacgcaat tcccaagacc ttgcaggaca atatttttga tttaactcag tttattctaa
    26941 ctaaaaagat ggatcaggcg cgcgatttgg tgagagactt gaccttgcaa ggggaagatg
    27001 aaatcaagct gattgcagtc atgctgggac aatttcggac ttttactcag gtgaagattt
    27061 tggcggagtc tggccaaaca gaatcgcaga ttgcaagtag tttaggtagt tatctgggac
    27121 gtaacccaaa tccttatcaa atcaagtttg cattaagaga ttcgagagga ctttctttga
    27181 gctttctgaa gcaagctatt tcctatttga ttgagacaga ctatcagatt aagacaggtc
    27241 tttatgaaaa aggtttcctt tttgaaaagg cactcttaca gattgctagt caggtcaatt
    27301 gacatttgtt gaaactacaa cctgcgtcag gattatcttg tcgtgggttt cttgctgatt
    27361 tgcttatttt attagaaagt atgtcagtga tttccttatc tataagcaaa aaacttgaaa
    27421 aaagtgaatg tttgcgttat cattttcata tagaaagaaa aagaggtatt atagatggct
    27481 attatcttac cagaacttcc atatgcatac gacgctttgg aaccatacat cgatgcggaa
    27541 acaatgcatt tgcaccatga caaacaccat caaacttatg tcaacaatgc caatgcagct
    27601 ttagaaaaac accctgaaat cggtgaagac cttgaagcct tgcttgctga tgtagaatct
    27661 atcccagctg atatccgtca agcacttatc aacaatggtg gcggacactt gaatcacgct
    27721 cttttctggg aattgatgac tcccgagaaa acagctcctt cagcagaact ggcagcagca
    27781 atcgatgcaa catttggttc atttgaagaa tttcaagcag ccttcactgc agcagcaaca
    27841 actcgttttg gttcaggttg ggcatggttg gttgtcaaca aagaagggaa acttgaagtg
    27901 acttcaacag caaaccaaga cacaccaatc tcagaaggta aaaaaccaat cttgggcttg
    27961 gacgtttggg aacatgctta ctacgtgaaa taccgcaacg tgcgtcctga ctatatcaaa
    28021 gctttctttt cagtaatcaa ctggaataaa gtagatgaat tgtacgcagc tgctaaataa
    28081 tgatagttgg agggaagaat tgttcttctc ttttttaggt tataagattc tggtctgaca
    28141 aaatcgtcag acttttttca tttttatgag aaacgtgcta actgctagaa attatggtag
    28201 aataaagtat taagtaatcg tggaaaaagg atatctatgc gaaaagaaat tgcacctgaa
    28261 ttatacaact ataacaagtt tcctggtccg gagttccatt tacacgggga caaggtcgaa
    28321 acggaaggga tagctttttc cttggttgaa aatatcaagg atgcctttga tgtgacgact
    28381 tttaatcagc gtttttcaga agtcttaacc aagtttgatt atatcgtggg ggactggagc
    28441 aacgaacagc ttcgcctacg aggtttttac aaggatgacc gaacagaaga aaaacttgaa
    28501 aaaatcagtc gtttacaaga ctacctttta gagtattgta gttatggttg tgcctatttt
    28561 gtcttagaaa atgaagcccc taagcgagca tcatttgaca agaaaatgcg taagacggaa
    28621 gaagaaacac cttctagaaa aggaaagaaa ccgactcaaa ccaaacgaaa gtcgaatgca
    28681 gataagaaaa atagacgtcg tcagaaagac cagcattctc agaaagagga caagggacaa
    28741 cgtcattttg tcattcgtca gaagtgagta gagaataagg agaagaaatg aaaccctcaa
    28801 ttcatagttt ggcacatcaa accatgcagg aatgggtatt ggagcaggga gaaaagaaat
    28861 tccgtgcaga tcaaatctgg gaatggctct accgtaaacg tgtgcagtca tttgaagaaa
    28921 tgaccaacct ttccaaggat ttgattgcta agctcaatga ccagtttgtg gtcaatccct
    28981 tgaaacaacg tattgtgcaa gagtctgctg atggtacagt caaatatctc tttgaattgc
    29041 ctgacggtat gttaattgag actgtactca tgcgtcaaca ctatggttta tctgtctgtg
    29101 tgaccactca ggtcggctgt aatatcggtt gtaccttctg tgcatctggt ttgattaaga
    29161 agcagcgtga cctcaataac ggtgaaatcg tagcgcaaat catgctggtt cagaaatact
    29221 ttgctgatga aggggttcag gtcaatcttg ccgtgtctct tcacgcaccc aacaatgaac
    29281 ttcgttcaag tatcatgaag attaaccgtg cctttccgat tgaaaaactc tttgctgcta
    29341 ttgaatacta cattgagaca acaaaccgtc gtgtaacctt tgaatatatc atgcttaatg
    29401 aagtcaacga cggtgtagaa caagccttgg aattggctga attgctcaag aacatcaaga
    29461 aattgtctta tgtaaacttg attccttata acccagttag tgagcatgac caatatagcc
    29521 gtagtcccaa agagcgcgtg ttggctttct atgatacgct taagaaaaaa gggggcaact
    29581 gtgtggttcg tcaagagcat ggtacagata ttgacgcagc ttgtggacaa ttgcgcttta
    29641 acacaatgaa acgtgaccgc cagaaggcag tcgcagcggt aaatccttaa tgatgaagaa
    29701 gacttataat catattttgg tctggggagt cattttctat agcatttgca ttgtctgttt
    29761 ttgctttact cctcaagaac aatctaccgt gggagtggga actccaggta ttcagcatct
    29821 tggacgcctg gtttttcttt tgactccttt caattctctc tggaaactgg gcgaagtgag
    29881 tgacattgga caattatgtt ggattttttt acaaaatatc ctcaatgtct tcttgttttt
    29941 tcctctgatt ttccaactcc tttatctatt tccaaatttg cggaaaacaa aaaaggtcct
    30001 tctttttagt tttcttgtga gtcttggaat cgagtgtacg caattaatct tggacttttt
    30061 ctttgatttc aatcgcgtct ttgagattga tgatttgtgg accaacactt tgggtggcta
    30121 tctggcttgg ctcctttata aacgattaca taaaaacaag gtaaggaatt aaaatgagta
    30181 ttttagaagt taaaaatctg agtcacggtt ttggtgaccg tgcaattttt gaagatgtgt
    30241 ccttccgtct cctcaaggga gaacatatcg gcctggtcgg tgccaatggt gaaggaaaat
    30301 caacctttat gagtatcgtg actggtaaaa tgctgccaga tgaaggaaag gttgagtggt
    30361 ccaaatatgt gacggctggt tacttggatc agcactctgt ccttgctgaa agacagtcgg
    30421 tgcgtgatgt tctccgtacg gcttttgatg agcttttcaa agctgaagct cgtatcaatg
    30481 acctctatat gaaaatggct gaagacggcg cggatgttga tgctctcatg gaagaagtag
    30541 gagaacttca agaccgtctg gagagtcgtg atttctatac cttggatgct aagattgacg
    30601 aagtagcgcg tgctcttggt gttatggact ttggcatgga tacggatgta acttctttgt
    30661 caggtgggca aagaaccaag gtgcttttgg caaaacttct ccttgaaaag cctgatatct
    30721 tgctgttgga cgagccgacc aactacttgg atgctgagca tattgattgg ctcaagcgct
    30781 atctccaaaa ctatgagaat gcctttgttc tcatttcgca cgatattcca ttcctcaatg
    30841 acgttattaa tattgtctat catgtggaaa atcaacagct gacgcgttac tctggtgact
    30901 actaccagtt ccaagaagtt tatgctatga agaaatctca gctagaggca gcctacgaac
    30961 gccagcagaa agagattgca gacctcaagg actttgtggc tcgtaataaa gcccgtgttg
    31021 caactcgtaa tatggctatg tctcgtcaaa agaaattgga taagatggat attatcgaac
    31081 tccaaagtga gaaaccaaaa ccatcctttg atttcaaacc agctcgtaca ccagggcgct
    31141 ttatcttcca agccaagaac ttgcaaattg gttacgaccg tcctcttact aagcctttaa
    31201 atcttacctt cgaacgcaat caaaaggttg cgattattgg tgctaatggt attggaaaaa
    31261 caactctctt gaagagtctc ttgggcatta tctcgccaat cgctggggaa gtggagcgtg
    31321 gagattattt agaacttggt tattttgagc aggaagtaga aggcggtaat cgccaaactc
    31381 ctcttgaagc tgtctggaat gcctttcctg cccttaatca agcagaagtc cgtgcagccc
    31441 ttgcccgttg tggtttgaca accaaacata ttgaaagcca gattcaagta ttatcagggg
    31501 gagagcaagc caaggttcgt ttctgtctct tgatgaatcg tgaaaacaac gttttagtgc
    31561 tggacgagcc gaccaaccat ttggatgtgg atgcaaagga tgagctcaaa cgcgctctca
    31621 aagaatatag gggatctatc cttatggtct gccacgagcc agacttttat gaaggctgga
    31681 tagaccaaat atgggatttt aataatttaa cttaagaatg agagtaaaag aaatacagta
    31741 ccgaatgtgg atactgtatt tttttggttt ttaagattta aaatcgtagt ctttcaccac
    31801 ttcgatgctg tcgattgtga tagcagtagt tggcttgtct ttttcatctt tttcggcctt
    31861 agcaatctta tccacaacat ccataccgtc aatcacttga ccaaagactg ggtgtttgcc
    31921 atctagacta gggtttccac cttctttgta ggcttcgata attttctgtg gatacttgct
    31981 tgtagggagt ttagaagagg tatctgtaga gctttggttg atgaagaact ggctgccatt
    32041 ggtgtttggt tgaccagtat tagccatagc aagagcacca cggatgttat acaaataagg
    32101 agtaatctcg ttcttgaaac cagttccttt gtctttagtc ttatccttgt catgccagat
    32161 ggactgacca cctgtaccgt ccccttttgg atctccagtt tggaccataa agccatcgat
    32221 gacacggtgg aaggtaatac cgttatagta gccttctttg gcatgagtga ggaaattttc
    32281 aaccgctaga ggagcgagtt tagggaagag tttaatgcga atatcacctt ggcttgtgtg
    32341 gagaatcact tcggcttcat cttcagcaac ttccttagat agttgtggga aattggcgtt
    32401 ttcgtttgtt aatgcatcgt ttaactcctt ggcagattgg gcagctactt tggaactttc
    32461 ttcagcagca agactggaat caacataatc atcaccacgc agactgcgtt ggacgctgct
    32521 acacccagct agggctacag tagacagtaa aagaagggtt gctagttttt tcattctgat
    32581 cctctcaaaa attcatttat aaccattgta ccctaaaagg aagtggattt caaatattag
    32641 tagtttacag aactggctta gataatagtt gggatttcat catcatcttg aaaatcttca
    32701 acttcttccc cagtaaatgg tttctgaaaa gaacttagta taaaacgggc aattggttca
    32761 acgattagga aatttagagg aagagctgca ataaagttta cgaaccaggc gcttagatag
    32821 gtattgggtg tgatagcaat gccattgtaa aaaagtccat agaaggacat gaaagtgacc
    32881 agctaattct tcttctgaaa aatgctcatg aagggcttga tccaggtcgc aagatttcat
    32941 tttagaaagt ccagtaactc ccttcttctt caggatattc tttttcatgg tattatttag
    33001 atggtcaagt gagtcaaaag aagtttcgac aagagcatac cctttttcta ccagagtttt
    33061 tagatgttta ggggcatcaa taccataagt gtactcaaag tattttggaa accagatctt
    33121 aagaaagctc gtaaagctag ccagttctct aaacgctaag aactagctac aacacagcat
    33181 ttacaacact tcatggttca caccatgggg tgttttttaa tgattttaag caaaattcac
    33241 acttaattga aagttattta aattagatga aaatttattt tttgaaaaag tcggaaaaca
    33301 ttgattctaa gcgaattttg aatcatattc gaatgggagt ttttaaaatc atgtttcagt
    33361 ggtaaaagta ccaaactgga ttctccatag taaaatgata tctcctgcta gtgaatcatc
    33421 tactaataag tccatatttc gcttgggaac gggcttggga cttagaagcc aagtatccaa
    33481 ctctcgttca ggcgatatga agggtttgac ttggtgtttt ttataaaatt tttctaaaat
    33541 agtattcacg gatagcatct ttcaaacatt tatttcctat tctaacaaat ttctaaatac
    33601 tttaagaaat ttatagtctg tcaagttttt ttcttgacac atgccagaaa tctgctataa
    33661 tagaacatgt gctaaatagc tcagctattt caccgaaata aaaaattaaa gaaatgagac
    33721 tttaaaaatg gcagttaaaa tccgtttgac tcgtatgggt tctaagaaaa aacctttcta
    33781 ccgtatcaac gtagcagatt cacgttcacc acgtgacgga cgtttcatcg aaacagttgg
    33841 aacttacaac ccacttgttg ctgaaaacca agtaactttg aaagaagatc gcgttcttgc
    33901 atggttggct aatggagctc aaccttcaga cacagtacgt aacatccttt caaaagaagg
    33961 cgtattgaaa aaattccacg attctaaatt ttcaaaataa gtttaaagta ggttgacaga
    34021 tggatacgat tgaaaatctc attattgcga ttgtgaaacc cttaatttca caaccagatg
    34081 ccttaactat caagattgag gatacaccag aatttttgga atatcatttg aatcttgatc
    34141 aaagcgatgt gggtcgtgta atcggtcgta agggtcgcac tatttctgcg ataagaacga
    34201 ttgtctactc tgtcccaact gaatacaaaa aagtaagaat cgttattgac gaaaaataag
    34261 aagggcggga cggatgtctc gcttttttgt gtggaataga agatatagta aaatgaaaca
    34321 agaacaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaaa
    34381 ggtgtactat tctagtttca atatactata aaagattgaa cgtttaggca attccaagac
    34441 tacttaatcg aacattaa
//
LOCUS       DAVHIT010000012        34399 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVHIT010000012 DAVHIT010000000
VERSION     DAVHIT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.012"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            508..960
                     /locus_tag="VVN16_000467"
     CDS             508..960
                     /locus_tag="VVN16_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU5571910.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            979..2316
                     /locus_tag="VVN16_000468"
     CDS             979..2316
                     /locus_tag="VVN16_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU5571911.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2395..3279
                     /locus_tag="VVN16_000469"
     CDS             2395..3279
                     /locus_tag="VVN16_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5571912.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3279..4112
                     /locus_tag="VVN16_000470"
     CDS             3279..4112
                     /locus_tag="VVN16_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5571913.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4130..6223
                     /gene="nanB"
                     /locus_tag="VVN16_000471"
     CDS             4130..6223
                     /gene="nanB"
                     /locus_tag="VVN16_000471"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU5571914.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKADFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6236..7339
                     /locus_tag="VVN16_000472"
     CDS             6236..7339
                     /locus_tag="VVN16_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5571915.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQNGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVVHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7513..8211
                     /locus_tag="VVN16_000473"
     CDS             7513..8211
                     /locus_tag="VVN16_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU5571916.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8230..9747
                     /locus_tag="VVN16_000474"
     CDS             8230..9747
                     /locus_tag="VVN16_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5571917.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9872..11200
                     /locus_tag="VVN16_000475"
     CDS             9872..11200
                     /locus_tag="VVN16_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5571918.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11453..12319
                     /locus_tag="VVN16_000476"
     CDS             11453..12319
                     /locus_tag="VVN16_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5571919.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12335..13174
                     /locus_tag="VVN16_000477"
     CDS             12335..13174
                     /locus_tag="VVN16_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5571920.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13447..13899
                     /locus_tag="VVN16_000478"
     CDS             13447..13899
                     /locus_tag="VVN16_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU5571921.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14041..14349
                     /locus_tag="VVN16_000479"
     CDS             14041..14349
                     /locus_tag="VVN16_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571922.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14636..15280
                     /locus_tag="VVN16_000480"
     CDS             14636..15280
                     /locus_tag="VVN16_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU5571923.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15449..16366
                     /locus_tag="VVN16_000481"
     CDS             15449..16366
                     /locus_tag="VVN16_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5571924.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16384..17268
                     /locus_tag="VVN16_000482"
     CDS             16384..17268
                     /locus_tag="VVN16_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5571925.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17284..18135)
                     /locus_tag="VVN16_000483"
     CDS             complement(17284..18135)
                     /locus_tag="VVN16_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU5571926.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18335..20392
                     /gene="pbp2b"
                     /locus_tag="VVN16_000484"
     CDS             18335..20392
                     /gene="pbp2b"
                     /locus_tag="VVN16_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU5571927.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20403..20999
                     /gene="recR"
                     /locus_tag="VVN16_000485"
     CDS             20403..20999
                     /gene="recR"
                     /locus_tag="VVN16_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU5571928.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21175..22218
                     /locus_tag="VVN16_000486"
     CDS             21175..22218
                     /locus_tag="VVN16_000486"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU5571929.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRPALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22302..23675
                     /locus_tag="VVN16_000487"
     CDS             22302..23675
                     /locus_tag="VVN16_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5571930.1"
                     /translation="MKLTIHEIAQVVGVKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGNQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23662..24273
                     /locus_tag="VVN16_000488"
     CDS             23662..24273
                     /locus_tag="VVN16_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5571931.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQGSDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24364..25068
                     /locus_tag="VVN16_000489"
     CDS             24364..25068
                     /locus_tag="VVN16_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU5571932.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25287..26660
                     /gene="ftsA"
                     /locus_tag="VVN16_000490"
     CDS             25287..26660
                     /gene="ftsA"
                     /locus_tag="VVN16_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5571933.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGETYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26677..27936
                     /gene="ftsZ"
                     /locus_tag="VVN16_000491"
     CDS             26677..27936
                     /gene="ftsZ"
                     /locus_tag="VVN16_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5571934.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27941..28612
                     /locus_tag="VVN16_000492"
     CDS             27941..28612
                     /locus_tag="VVN16_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5571935.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28622..29161
                     /locus_tag="VVN16_000493"
     CDS             28622..29161
                     /locus_tag="VVN16_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU5571936.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29161..29424
                     /locus_tag="VVN16_000494"
     CDS             29161..29424
                     /locus_tag="VVN16_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5571937.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29421..30206
                     /locus_tag="VVN16_000495"
     CDS             29421..30206
                     /locus_tag="VVN16_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU5571938.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGIACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELNLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLFQDKGQTKKEKKKITVQLLLSK"
     gene            30215..31003
                     /gene="divIVA"
                     /locus_tag="VVN16_000496"
     CDS             30215..31003
                     /gene="divIVA"
                     /locus_tag="VVN16_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU5571939.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31351..34143
                     /locus_tag="VVN16_000497"
     CDS             31351..34143
                     /locus_tag="VVN16_000497"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5571940.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTSANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPTPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        10585 a         6072 c         7483 g        10259 t
ORIGIN      
        1 ttggtgaaac gagatgtaga aaggagattt agccaaagag tctattagtg ctagaataat
       61 agattagaat tattttagaa aaacgaagtg agcagcttat aaattcaagt ccccaaatag
      121 attcatacta gtatcttttg caaaaaataa agggcgactt ccttcatgaa tatcaatttc
      181 atctataagg aaggtagcta attgaactaa cttatttatt ctgtttgtcg ctagaaaaat
      241 cagacctcct tgtgaagatt gaggagatac tgaatgaaaa tcaaagaaga aactagcaag
      301 ctagtagcag attgcccaaa acaccgcttt gaggttgtag ataagactga cctatataat
      361 ccaaggtgaa gcgactgtgg tttgaagaga ttttcaaaga gtataggcta gagagtagtg
      421 tttttatgtc cttctagtag aaaatgctag acagaagaat ggggaacttg gataggaaaa
      481 atagattgag aaaggaggtt agaagagatg attattacaa aaattagccg tttaggaact
      541 tatgtgggag taaatccaca ttttgcaaca ttaatagatt ttctagaaaa aacaggacta
      601 gaaaatttaa cagaaggttc gattgctatc gatggtaatc gattgtttgg gaattgcttt
      661 acttatctag cagatggtca agcaggggct ttctttgaaa cacaccaaaa atatttggat
      721 attcatttag ttttggaaaa cgaagaagcc atggctgtta catcgccgga aaatgtaagc
      781 gttacccaag aatatgatga agagaaagat attgaattat acacagggaa agtggaacag
      841 ttggttcatt tgagagctgg cgaatgcctc atcacttttc cagaagattt acatcaaccc
      901 aaggttcgta taaatgatga acctgtgaaa aaagttgtct ttaaagttgc gatttcttaa
      961 tgtagaaaga gaagaacgat gaaaaaaatg agaaagtttt tatgtctagc tggaattgcg
     1021 ctagcggctg ttgccttggt agcttgttca ggaaaaaaag aagctacaac tagtactgaa
     1081 ccaccaacag aattatctgg tgagattaca atgtggcact cctttactca aggaccccgt
     1141 ttagaaagta ttcaaaaatc agcagatgct ttcatgcaaa agcatccaaa aacgaaaatc
     1201 aagattgaaa cattttcttg gaatgacttc tatactaaat ggactacagg tttagcaaat
     1261 ggaaatgtgc cagatatcag tacagctctt cctaaccaag taatggaaat ggtcaactca
     1321 gatgctttgg ttccgctaaa tgattctatc aagcgtattg gacaagataa atttaacgaa
     1381 actgccttaa atgaagcaaa aatcggagat gattactact ctgttcctct ttattcacat
     1441 gcacaagtca tgtgggttag aacagatttg ttaaaagaac ataatattga ggttcctaaa
     1501 acttgggatc aactctatga agcttctaaa aaattgaaag aagctggagt ttatggcttg
     1561 tctgttccgt ttggaacaaa tgacttaatg gcaacacgtt tcttgaactt ctacgtacgt
     1621 agtggtggag gaagcctctt aacaaaagat cttaaagcag acttgacaag ccaacttgct
     1681 caagatggta ttaaatactg ggttaaattg tataaagaaa tctcacctca agattctttg
     1741 aactttaatg tccttcaaca agctaccttg ttctatcaag gaaaaacagc atttgacttt
     1801 aactctggct tccatatcgg aggaattaat gccaacagtc ctcaattgat tgattcgatt
     1861 gatgcttatc ctattccaaa aatcaaagag tctgataaag accaaggaat tgaaacctca
     1921 aacattccaa tggttgtttg gaaaaattca aaacatccag aagttgctaa agcattctta
     1981 gaagcacttt ataatgaaga agactacgtt aaattccttg attcaactcc agtaggtatg
     2041 ttgccaacta ttaaggggat tagcgattct gcagcctata aagaaaatga aactcgtaag
     2101 aagtttaaac atgctgaaga agtaattact gaagctgtta aaaaaggtac tgctattggt
     2161 tatgaaaatg gaccaagtgt acaagctggt atgttgacta accaacacat tattgaacaa
     2221 atgttccaag atatcattac aaatggaaca gatcctatga aagcagcaaa agaagcagaa
     2281 aaacaattaa acgatttatt tgaggctgtt cagtagatgt aaaagactag aaaataggtg
     2341 ggctagtgag ctgaaaagct ctagcccaat cttgtaaaag aagggagaag gagaatggtt
     2401 aaagaacgta atttaactcg ctggatattt gttttgccag ctatgattat cgtaggatta
     2461 ctctttgttt atccgttttt ctcgagtatt ttttatagct ttaccaataa gcatttgatt
     2521 atgcctaatt ataaatttgt tggtttggct aactataaag ctgtgctatc agatcccaac
     2581 ttctttaatg cgttctttaa ttcaattaag tggaccgttt tctcattagt tggtcaagtt
     2641 ttagtagggt ttgtattggc tttagctctt cacagagtac gccacttcaa gaaattatat
     2701 aggaccttat tgattgttcc ttgggcattt cctacaatcg ttattgcctt ctcttggcag
     2761 tggattctaa acggggttta tggctactta cctaatctaa tcgtaaaatt aggtttaatg
     2821 gaacatacac ctgcattttt gacagatagt acatgggcat tcctatgttt ggtgtttatc
     2881 aacatttggt ttggagcacc aatgattatg gttaatgtgc tttcagcttt gcaaacagta
     2941 ccagaagaac aatttgaggc tgctaagata gatggtgctt caagttggca ggtgttcaag
     3001 tttatcgtct ttccacatat taaagtggtt gtaggacttc tagttgtttt gagaactgta
     3061 tggatcttta ataactttga cattatctac ctcattactg gtggtggacc agccaatgct
     3121 acaacgacgc ttccaatttt tgcttacaac ctgggttggg gaactaaatt gttgggtcgt
     3181 gcttcagcag ttacagtatt gctctttatc ttcttggtgg cgatttgctt tatctacttt
     3241 gctatcatca gtaagtggga aaaggagggt agaaaataat gaagaagaaa tccagtattt
     3301 atttagatat tctctcacat gtacttttag ttggtgcgac catcgttgca attttcccat
     3361 tggtatggat tatcatatct tctgtcaaag ggaaagggga attaactcag tatccaacac
     3421 gattttggcc tgaacagttt acattagatt atttcactca tgttatcaac gatttgcact
     3481 tcattgataa cattcgaaac agtttaatca ttgccttggc tacaaccctc attgcgatta
     3541 ttatttctgc tatggcagcc tatggtattg ttcgattctt tcctaaattg ggagcaatca
     3601 tgtcgagact actcgtcatt acctacattt tcccaccaat tttgttagca attccctatt
     3661 caattgccat tgctaaagtt gggttaacaa atagtttatt tggcttgatg atggtttatc
     3721 tatcttttag tgttccatat gcagtttggc tcttagttgg atttttccaa acagttccaa
     3781 ttggaattga agaagcggct agaattgatg gtgcaaataa atttgttacg ttttataagg
     3841 ttgtgctacc gattgtagca ccaggtattg tagcaacagc tatttataca tttatcaatg
     3901 cttggaatga attcctgtat gccttgattt tgattaacaa tacaggaaag atgacagtag
     3961 cagtagccct tcgttcactt aatggttcag aaatactaga ctggggagat atgatggcag
     4021 cgtctgttat tgtagttctt ccatcaatta ttttcttctc tatcatccaa aataagattg
     4081 caagtggatt atcagaagga tctgtgaagt agacgaaaga aggaaaaaaa tgaataaaag
     4141 aggtctttat tcaaaactag gaatttctgt tgtaggcatt agtcttttaa tgggagttcc
     4201 cactttgatt catgcgaatg aattaaacta tggtcaactg tccatatctc ctatttttca
     4261 aggaggttca tatcaactga acaataagag tatagatatc agctctttgt tattagataa
     4321 attgtctgga gagagtcaga cagtagtaat gaaatttaaa gcagatgaac caaactctct
     4381 tcaagctttg tttggcctat ctaatagtaa agcagacttt aaaaataatt acttttcaat
     4441 tttcatgaga gattctggtg agataggtgt agaaataaga gacgcccaaa agggaataaa
     4501 ttatttattt tccagaccag cttcattatg gggaaaacat aaaggacagg cagttgaaaa
     4561 tacactagta tttgtatctg attctaaaga taaaacatac acaatgtatg ttaatggaat
     4621 agaagtgttc tctgaaacag ttgatacatt tttgccaatt tcaaatataa atggtataga
     4681 taaggcaaca ctaggagctg ttaatcgtga aggtaaggaa cattacctcg caaaaggaag
     4741 tattgatgaa atcagtctat ttaacaaagc aattagtgat caggaagttt caaatattcc
     4801 cttgtcaaat ccatttcagt taattttcca atcaggagat tctactcaag ctaactattt
     4861 tagaataccg acactatata cattaagtag tggaagagtt ctatcaagta ttgatgcacg
     4921 ttatggtggg actcatgatt ctaaaagtaa gattaatatt gccacttctt atagtgatga
     4981 taatgggaaa acgtggagtg agccaatttt tgctatgaag tttaatgact atgaggagca
     5041 gttagtttac tggccacgag ataataaatt aaagaatagt caaattagtg gaagtgcttc
     5101 attcatagat tcatccattg ttgaagataa aaaatctggg aaaacgatat tactagctga
     5161 tgttatgcct gcgggtattg gaaataataa tgcaaataaa gccgactcag gttttaaaga
     5221 aataaatggt cattattatt taaaactaaa gaagaatgga gataacgatt tccgttatac
     5281 agttagagaa aatggtgtcg tttatgatga aacaactaat aaacctacaa attatactat
     5341 aaatgataag tatgaagttt tggagggagg aaagtcttta acagtcgaac aatattcggt
     5401 tgattttgat agtggctctt taagagaaag gcataatgga aaacaggttc ctatgaatgt
     5461 tttctacaaa gattcgttat ttaaagtgac tcctactaat tatatagcaa tgacaactag
     5521 tcagaataga ggagagagtt gggaacaatt taagttgttg cctccgttct taggagaaaa
     5581 acataatgga acttacttgt gtcctggaca aggtttagca ttaaaatcaa gtaacagatt
     5641 gatttttgca acatatacta gtggagaact aacctatctc atttctgatg atagtggtca
     5701 aacatggaag aaatcctcag cttcaattcc gtttaaaaat gcaacagcag aagcacaaat
     5761 ggttgaactg agagatggtg tgattagaac attctttaga accactacag gtaagatagc
     5821 ttatatgact agtagagatt ctggagaaac atggtcggaa gtttcgtata ttgatggaat
     5881 ccaacaaact tcatatggca cacaagtatc tgcaattaaa tactctcaat taattgatgg
     5941 aaaagaagca gtcattttga gtacaccaaa ttctagaagt ggccgtaagg gaggtcaatt
     6001 agttgtcggt ttagtcaata aagaagatga tagtattgat tggaaatacc actatgatat
     6061 tgatttgcct tcgtatggtt atgcctattc tgcgattaca gaattgccaa atcatcacat
     6121 aggtgtactg tttgaaaaat atgattcgtg gtcgagaaat gaattgcatt taagcaatgt
     6181 agttcagtat atagatttgg aaattaatga tttaacaaaa taaaggagaa aaaacatggt
     6241 taaatacggt gttgttggag cagggtattt tggagctgaa ttggctcgct atatgcaaaa
     6301 gaatgatgga gcagagatta ctcttctcta tgatccagat aatgcagagg cgattgcaga
     6361 agaattggga gcaaaagtag caagttcctt agatgagttg gtttctagcg atgaagtaga
     6421 ttgtgttatc gtcgcaactc caaataatct tcataaggaa ccggttatta aggctgcaca
     6481 gaatggtaaa aatgttttct gtgaaaaacc aattgcgctt tcttatcaag attgtcgcga
     6541 gatggtagat gcatgtaaag aaaacaatgt aacctttatg gcaggacata ttatgaattt
     6601 ctttaatggt gttcatcatg caaaagaact cattaatcaa ggagttatcg gagacgttct
     6661 atattgtcat acagctcgta atggttggga agaacaacaa ccgtcagtat catggaaaaa
     6721 aattcgtgaa aaatcaggtg gtcacttgta tcaccacatc catgaattgg attgcgttca
     6781 attccttatg gggggcatgc ctgaaactgt aaccatgaca ggtggaaatg tggtccatga
     6841 aggtgaacat ttcggtgatg aagatgatat gatttttgtc aatatggaat tttctaataa
     6901 gcgttttgcc ttgttagaat ggggttcagc ttatcgttgg ggtgaacatt atgtcttaat
     6961 ccaaggaagc aaaggtgcca tccgcttaga cttattcaac tgtaaaggaa ctcttaagct
     7021 agatgggcaa gaaagctatt tcttgattca cgaatcgcaa gaagaagatg atgatcggac
     7081 tcgtatctat catagtacag agatggatgg agcaattgct tatggtaaac caggtaaacg
     7141 tactccatta tggctatcat ctgtcattga taaagaaatg cgctatctgc atgagattat
     7201 ggaaggagct ccagtatcag aagaatttgc aaaacttttg acaggtgaag ctgccctaga
     7261 agcaattgct actgcagatg cttgtaccca gtctatgttt gaagatcgca aagtaaaatt
     7321 gtcagaaatt gtaaaataaa ttttggtatt ctcctattta taggtcgact tgctcctctg
     7381 aaagtacttt tagaggagct gtttgacttt gctagttttt gaaactgaaa tctattatac
     7441 tacaaactat tgaaagcgtt ttaattttaa ggtataataa tctcatagaa ataaagaaaa
     7501 ggaggaaaga ggatgccaca gattagcaaa gaagccttga ttgagcaaat caaagatgga
     7561 atcatcgttt cttgtcaggc tcttcctcat gaaccgcttt atacagaagc gggaggggtg
     7621 attcccttgc tggtcaaagc ggctgagcaa ggtggagcag tcggtatccg agcaaacagt
     7681 gttcgcgata tcaaggaaat taaggaagtc actaaacttc caatcattgg gattatcaaa
     7741 cgtgattatc cacctcagga acccttcatc acggctacta tgaaagaagt tgatgaattg
     7801 gcagaactgg acatcgaggt gattgctctg gattgtacca agcgtgaacg ctacgatggt
     7861 ttggaaattc aagagttcat tcgtcaggtt aaggagaaat atcctaatca gcttttgatg
     7921 gctgatacta gtatcttcga agaagggcta gcagctgtag aagcaggaat tgactttgtc
     7981 ggaacaacct tatcaggcta cacatcctac agtccaaaag tagacggtcc agattttgaa
     8041 ttgattaaga aactctgtga tgccggtgta gatgtcattg cagaaggaaa aattcataca
     8101 ccagaacaag ccaaacaaat ccttgaatat ggagtgcgag gcatcgttgt tggtggcgct
     8161 attactagac caaaagagat tacagaacgc ttcgttgcta gtcttaaata agatgtgagg
     8221 gggagtttta tgtttaaagt tttacaaaaa gttggaaaag cttttatgtt acctatagct
     8281 atacttcctg cagcaggtct acttttgggg attggtggtg cactttcaaa cccaaccacg
     8341 atagcaactt atccaatact agacaatagt atttttcaat cgatattcca agtaatgagc
     8401 tctgcaggag aggttgtatt cagtaatttg tcactacttc tctgtgtggg attatgtatt
     8461 ggcttagcga aacgagataa aggaaccgct gcgttagcag gagtaactgg ttacttagtt
     8521 atgactgcaa cgatcaaagc tttggtaaaa ctttttatgg cagaaggatc tgcaattgat
     8581 actggagtta ttggagcatt agttgtcgga atagttgccg tatatttgca caaccgatat
     8641 aacaatattc aattaccttc cgctttagga ttctttggag gttcacgctt cgttcctatt
     8701 gttacatcgt tctcttctat cttgattggc tttgtcttct ttgttatttg gccacctttc
     8761 caacaacttc ttgtttctac aggtggatat atttctcagg cgggtccaat tggaactttt
     8821 ctatatggat ttttaatgag actttctgga gcagtaggct tacatcatat aatttaccct
     8881 atgttttggt atactgaact tggtggtgtt gaaactattg caggacaaac agtggttgga
     8941 gctcaaaaaa tattttttgc tcaattagcc gatccggccc attctggatt atttacagaa
     9001 ggaacaaggt tttttgcagg tcgtttctca acaatgatgt tcggtttacc ggctgcctgt
     9061 ttagcgatgt accatagtgt tcctaaaaat cgtcgtaaaa aatacgcggg tttgtttttt
     9121 ggagttgctt taacatcttt tattaccggt attacagaac caattgaatt tatgtttcta
     9181 ttcgtcagtc cggttctata tgttgttcac gcattccttg atggtgttag cttctttatt
     9241 gcagacgtct taaatatttc aataggaaac acattttcag gaggtgtaat cgatttcaca
     9301 ttatttggaa ttttgcaggg gaacgctaag acgaattggg ttcttcagat tccaattgga
     9361 cttatttgga gtgttttgta ttatattatt tttagatggt tcattactca attcaacgtt
     9421 ctaacgccag gacgaggaga agaagtagat tctaaagaaa tttctgaatc cgcagattca
     9481 acttcaaata ctgcagatta tttaaaacag gatagcctac aaattatcag agccttgggt
     9541 ggatcaaata atatagaaga tgtagatgct tgtgtgacac gtttacgtgt agctgtaaaa
     9601 gaagttaatc aagttgataa agcactttta aaacaaattg gtgcagttga tgtcttagaa
     9661 gtgaagggtg gcattcaagc aatctatgga gcaaaagcaa tcttatataa aaatagtatt
     9721 aatgaaattt taggtgtaga tgattaagta cttactgact taataaaaaa cagaggagag
     9781 tgatggatga gtaggatgaa atgaaatcgc atacaagaaa taaagaactc attatccaag
     9841 ttggatacgc ttattacata ggagaataca aatgaaattt agaaaattag cttgtacagt
     9901 acttgcgggt gctgcggttc ttggtcttgc tgcttgtggc aattctggcg gaagtaaaga
     9961 tgctgccaaa tcaggtggtg acggtgccaa aacagaaatc acttggtggg cattcccagt
    10021 atttacccaa gaaaaaactg gtgacggtgt tggaacttat gaaaaatcaa tcatcgaagc
    10081 gtttgaaaaa gcaaacccag atataaaagt gaaattggaa accatcgact tcaagtcagg
    10141 tcctgaaaaa atcacaacag ccatcgaagc aggaacagct ccagacgtac tctttgatgc
    10201 accaggacgt atcatccaat acggtaaaaa cggtaaattg gctgagttga acgacctctt
    10261 cacagatgaa tttgttaaag atgtcaacaa tgaaaacatc gtacaagcaa gtaaagctgg
    10321 agacaaggct tatatgtatc cgattagttc tgccccattc tacatggcaa tgaacaagaa
    10381 aatgttagaa gatgctggag tagcaaacct tgtaaaagaa ggttggacaa ctgatgattt
    10441 tgaaaaagta ttgaaagcac ttaaagacaa gggttacaca ccaggttcat tgttcagttc
    10501 tggtcaaggg ggagaccaag gaacacgtgc ctttatctct aacctttata gcggttctgt
    10561 aacagatgaa aaagttagca aatatacaac tgatgatcct aaattcgtca aaggtcttga
    10621 aaaagcaact agctggatta aagacaattt gatcaataat ggttcacaat ttgacggtgg
    10681 ggcagatatc caaaactttg ccaacggtca aacatcttac acaatccttt gggcaccagc
    10741 tcaaaatggt atccaagcta aacttttaga agcaagtaag gtagaagtgg tagaagtacc
    10801 attcccatca gacgaaggta agccagctct tgaatatctt gtaaacgggt ttgcagtatt
    10861 caacaataaa gacgacaaga aagtcgctgc atctaagaaa ttcatccagt ttatcgcaga
    10921 tgacaaggag tggggaccta aagacgtagt tcgtacaggt gctttcccag tccgtacttc
    10981 atttggaaaa ctttatgaag acaaacgcat ggaaacaatc agtggctgga ctcaatacta
    11041 ctcaccatac tacaacacta ttgatggatt tgctgaaatg agaacacttt ggttcccaat
    11101 gttgcaatct gtatcaaatg gtgacgaaaa accagcggat gctttgaaag ccttcactga
    11161 aaaagcaaac gaaacaatca aaaaagctat gaaacaatag tccttagtta ttctatgaaa
    11221 aagtaatttt ttaaagaacc taagagtgta tacccccttt tccctctaca cagatagtgt
    11281 aagaaaaggg ggcttttgtt taaaatgtaa gaaactgtca cgaaattaaa atgaagttct
    11341 tacataagcg aaccataaaa aatttcattt tgattttaaa acagttcaag aaagtcaaaa
    11401 aattattcta tttgaaagag aggtgccgac tgtgaaagtc aataaaatcc gtatgcggga
    11461 aacagtgatt tcctacgctt tcctagcacc agtattattc ttctttgtca tctttgtgtt
    11521 ggctccgatg gtgatgggct tcattacaag tttctttaac tactcaatga ctaaatttga
    11581 gtttgtaggc ttggataact atatccgtat gtttaaagat cctgtcttta caaaatctct
    11641 gattaacaca gttattttgg ttattggatc tgtaccagtt gttgttctat tctcactctt
    11701 tgtagcatct cagacctatc atcaaaatgt cattgccaga tccttctacc gtttcgtctt
    11761 cttccttcct gttgtaacgg gtagtgttgc cgtgacagtt gtttggaaat ggatttatga
    11821 cccactatca gggattctaa actttgtcct taagtcaagc cacatcatca gccaaaacat
    11881 ttcttggttg ggagataaaa actgggcatt gatggcgatt atgattattc tcttgaccac
    11941 ttcagttggt cagcccatca tcctttatat cgctgccatg gggaatattg acaattcact
    12001 ggttgaagcg gcgcgtgttg atggtgcaac tgagtttcaa gttttttgga agattaaatg
    12061 gccaagcctt cttccaacaa ctctttatat tgcaatcatc acaacaatta actcattcca
    12121 gtgtttcgcc ttgattcagc ttttgacatc tggtggtcca aactactcaa caagtacctt
    12181 gatgtactac ctttacgaaa aagccttcca attgacagaa tacggctatg ccaacacaat
    12241 tggtgtcttc ttggcagtca tgattgctat cgtaagcttt gttcaattta aagtacttgg
    12301 aaacgacgta gaatactaaa gaaaggagac agctatgcaa tctacagaaa aaaaaccatt
    12361 aacagccttt actgttattt caacaatcat tttgctcttg ttgactgtgc tgttcatctt
    12421 tccattctac tggattttga caggggcatt caaatcacaa cctgatacaa ttgttattcc
    12481 tcctcagtgg ttccctaaaa tgccaaccat ggaaaacttc caacaactca tggtgcagaa
    12541 ccctgccttg caatggatgt ggaactcagt atttatctca ttggtaacca tgttcttagt
    12601 ttgtgcaacc tcatctctag caggttatgt attggctaaa aaacgtttct atggtcaacg
    12661 cattctattt gctatcttta tcgctgctat ggcgcttcca aaacaagttg tccttgtacc
    12721 attggtacgt atcgtcaact tcatgggaat ccacgatact ctctgggcag ttatcttgcc
    12781 tttgattgga tggccattcg gtgtcttcct catgaaacag ttcagtgaaa atatccctac
    12841 agagttgctt gaatcagcta aaatcgacgg ttgtggtgag attcgtacct tctggagtgt
    12901 agccttcccg attgtgaaac cagggtttgc agcccttgca atctttacct tcatcaatac
    12961 ttggaatgac tacttcatgc agttggtaat gttgacttca cgtaacaatt tgaccatctc
    13021 acttggggtt gcgaccatgc aggctgaaat ggcaaccaac tatggtttga ttatggcagg
    13081 agctgccctt gctgctgttc caatcgtcac agtcttccta gtcttccaaa aatccttcac
    13141 acagggtatt actatgggag cggtcaaagg ataatactct gcgaaaatcg aatgcaaact
    13201 acgtcagctt caccttgcca tacttaagta ttgcctgtgg ttagcttcct agtttgttct
    13261 tcaattttca ttgagtatag gaaaatcaat ctatcaaaat acagaagtat attttataga
    13321 tttagagaat atagaagtta taagtgtcta caaaatggag ggtatgcagt tactttatga
    13381 agttttgtca gacacttatc aacttaagaa tggttttagt taactatcag aaacgaagga
    13441 aagagtatga tttttgacga tttgaaaaac atcacctttt acaaagggat tcatcccaat
    13501 ttagacaagg ctatcgacta tctctaccaa catcgtaaag attcattcga attaggaaag
    13561 tatgagattg atggagataa agtctttcta gttgttcagg aaaatgtcct caatcaagtt
    13621 gagaataatc aatttgaaca ccataagaac tatgcagatt tgcatttgct ggtagaagga
    13681 catgaatatt cgagctacgg ttcacgtatc aaagacgagg cagtagcatt cgacgaagcg
    13741 agtgacattg gctttgttca ttgtcatgaa cactacccac tcttgttggg ttatcacaat
    13801 tttgcgattt tcttcccagg tgagccacat cagccaaatg gttatgcagg catggaagaa
    13861 aaggttcgaa aatatctctt taaaattttg attgattaaa aataggatga attgtttttt
    13921 tgtaaagctt tgacaatact ctaccatgaa attgatcttt gtgaggtagg gaaatgagaa
    13981 taaaatattt aaaaattggt atcttcttgt cagtctgtgt atgctacaag agctagtttc
    14041 ttggatggac agggaattac agttgatgag atggcttgga taattagggg cattgtgaat
    14101 gcattgattg gtagatacat aaaattaggt acttatgcgg ctaagtatgg tattagtatg
    14161 gcgcgttcta tcttaaatag ggcagctgca actgcggcag caagagtagg attattgact
    14221 aagatttttg gatggatttt acgaatagct gtgctcgcag ctgatgtata tggtaatttt
    14281 gccaacaata ttgctgcagc ttgggatgca catgataaaa tccctaacaa tggtcgtata
    14341 aacttttaaa atgcgagaat gaaagcactt tgtatttttt tattgaatat gttagcttgg
    14401 acagtgcttg caatgatagt ccgtggaggg ctagatggat ttaatgagca tacttggagt
    14461 actattttaa ttgcttcgct gtccgggata tatatagatt atatgtccat agataaaagt
    14521 agaaaaaagt ccaaaagaaa aaagaaaaaa tagatttgtt catggtaggg acttatgaaa
    14581 gctttactga caaaaaagaa aacagtttac aaagaaaaat gatggaggag caaacatggc
    14641 acaaaaagga gtaagcctta tcaaggcagc atttgataca gacaactttc tcatgcgttt
    14701 tagtgagaaa gtcttggaca tcgtgacagc caatcttctt tttgtcgtct cttgtttgcc
    14761 catcgtgacg attggagtgg ctaaaatcag cctctacgag accatgttcg aagttaagaa
    14821 gagcagacgg gtgcctgttt ttaaaatcta tctaagatct ttcaagcaaa atctgaaact
    14881 aggtcttcag ctgggtttaa tggagttagg aattgtgttt cttacccttt cagatctcta
    14941 tcttttctgg ggtcaaacag ctctgccctt ccaattgctg aaagccattt gtttaggtat
    15001 tctgattttt cttactatcg tgatgctggc tagttaccct atcgcggcac gttatgacct
    15061 atcttggaaa gaaattcttc aaaaaggatt gatgttggct agttttaact ttccttggtt
    15121 cttcctcatg ttagccattc ttgtcctcat tgtgatggtt ctttatctgt ccgccttcag
    15181 tctactctta ggtggctcag tcttcttact ttttgggttt ggactattgg tctttatcca
    15241 gactggattg atggagaaaa ttttcgcaaa ataccaatag gagccttatt tctgaaacta
    15301 ctttcaaacg ctccaaacgc tattctataa gcgagaaact aaaatctaag tgtctataag
    15361 atattgaaag ccttttcggt aaagtgtata ctaaagaagt aaagaaaaat ctgctttagc
    15421 agaaaataaa aataatagga gaaaatctat gtcagatttg aaaaaatacg aaggtgtcat
    15481 tccagccttc tacgcatgtt atgatgatca aggagaagta agcccagaac gtacgcgtgc
    15541 cttggttcaa tacttcattg ataaaggtgt tcaaggtctt tatgtcaatg gttcttctgg
    15601 tgaatgtatc taccaaagcg ttgaagatcg caagttgatt ttggaagaag tcatggcggt
    15661 agccaaaggt aaattgacca tcattgctca tgttgcctgc aacaatacta aagatagtat
    15721 ggaacttgct cgccatgctg aaagcttggg agtagatgct attgcaacga ttccaccaat
    15781 ttatttccgc ttgccagaat actcagttgc caaatactgg aacgatatca gttctgcagc
    15841 tccaaacaca gactacgtga tttacaacat tcctcaattg gcaggggttg ctttgactcc
    15901 aagcctttac acagaaatgt tgaaaaatcc tcgtgttatc ggtgttaaga actcttctat
    15961 gccagttcaa gatatccaaa cctttgtcag ccttggtggg gaagaccata tcgtctttaa
    16021 tggtcctgat gagcagtttc taggaggacg cctcatgggt gctagggctg gtatcggtgg
    16081 tacttatggt gctatgccag aactcttctt gaaactcaat cagttgattg cggataagga
    16141 cctagaaaca gcgcgtgaat tgcagtatgc tatcaacgca atcattggta aactcacttc
    16201 tgctcatgga aatatgtacg gtgtcattaa agaagtcttg aaaatcaatg aaggcttgaa
    16261 tattggatct gttcgttcac cattgacacc agtgactgaa gaagatcgtc cagttgtaga
    16321 agcggctgct gccttgattc gtgaaaccaa ggagcgcttc ctctaatcta aaaggaggta
    16381 tttatgacat actacgttgc aattgatatc ggtggaacca acatcaagta tggtttggtt
    16441 gatcaagagg ggcaacttct tgaatcgcat gaaatgccaa ctgaggcgca taagggtgga
    16501 cctcatatct tacaaaagac caaagatatc gtagctagtt atttagaaaa aggcccagta
    16561 gcaggtgttg ccatatcttc tgctgggatg gtggatccgg ataagggtga gattttctat
    16621 gctgggcctc aaatccctaa ctacgcaggc acccagttca aaaaggaaat cgaagaaagc
    16681 tttactattc cttgtgagat tgaaaatgat gtcaactgtg caggtcttgc tgaggcagta
    16741 tctggttcag gcaagggagc aagtgtgaca ctttgcttga ccattggaac cggtatcggt
    16801 ggttgcttga ttatggatag gaaagtcttc catggtttta gcaattcagc ctgtgaagtc
    16861 gggtatatgc atatgcagga tggagctttt caggatctcg cttctacaac agctttagtg
    16921 aaatatgtag ctgaagccca tggagaagat gttgatcagt ggaatggccg acgtatcttt
    16981 aaggaagcta ctgaaggaaa caaaatctgc atggaaggta ttgaccgtat ggttgactat
    17041 ctaggaaaag gtctggcaaa tatttgttat gtggccaatc cagaagtggt tattcttggt
    17101 ggtggtatca tggggcaaga ggctatcctc aaacctaaga tccgcacagc cttgaaagag
    17161 gcattggtac caagtttagc agaaaaaaca cgattagaat ttgcccatca ccaaaataca
    17221 gcagggatgt tgggtgcata ttatcatttt aagacaaaac aatcctagtt tggctcagcc
    17281 aaactaggat tttcttacac gtttttgtct acgatagccg ttgagttttt tattttccca
    17341 gtagctatta aagatttttt ccttgctttc gcgattgatt tccaaaaagt aggcataaat
    17401 caaatcgata aagaagagca taggaagttg agcggatatt cgttggatgt aggagggttg
    17461 gctgtgggtg gctacaagaa cagtctctgt ataggtctgg ctatctttat tgggaacact
    17521 tgtaaagagt acagtctttg cccccatctc cttggcatct aatagactat ctaaaataga
    17581 aggagttgag cctgaaagtg agaaacccag tactagacaa ttttcatcca tgatgctggt
    17641 tgtccaggca aagccgtctt ggtctgtcaa agcttcgcag accacaccta gtcgcataaa
    17701 acgtaatttc atttcacggg cgacgaggcc agaactccct gttccaaaga agtagatacg
    17761 ctcagcatct tcgattagct gggcaattcg ctctagttgg atttcgtcaa tcaagtcttg
    17821 tgtttgttcc ctcatattgc tataacttct gaggactcgt ttggtcagtg gactatgctt
    17881 ggagacttgg ttggcttgat tttctgcctg atgttggtat tggaaaataa attctcggta
    17941 gccagtaaag ccacactttt tagcaaagcg ggtcaaagca gcttgagaaa tatgtaattt
    18001 ttgggtgact tgttgagaag ataaatcatc tgtaatcgtt tcagcttgca aaaaatagcg
    18061 agcgatttct tgctctaggt ctgtcatttc ttcaaaatgt gaatcaatga tagttgcgat
    18121 atctggtttg tccataggga aagctccttt acatgggtca tactggaaga ctagatcagg
    18181 gaatagtcac acttcattat aacacataat ataaggatag ataaataaaa acgcatctct
    18241 gttttaaaaa cgaaaaaatc gaaaaagctt ctctcttttc cataattttc tactcaaatt
    18301 gtggtacaat taagagtaag attttaagtt agaaatgaga ctgatttgta tgagaaaatt
    18361 taacagccat tcgattccga ttcggcttaa tttattgttt tcaatcgtca ttttactctt
    18421 tatgaccatt attggtcgtt tgttgtatat gcaggttttg aacaaggatt tttacgaaaa
    18481 aaagctagcc tcagctagtc agaccaagat tacaagcagt tcagcccgtg gggaaattta
    18541 tgatgctagt ggaaaacctt tggtagaaaa tacgttaaag caggttgttt cctttacgcg
    18601 tagcaataaa atgacggcta cagacttaaa agaaacagct aaaaagttac tgacttatgt
    18661 gagcatcagt tctccaaatt tgacagaacg ccagctggcg gattactatt tggctgatcc
    18721 tgaaatctat aaaaaaacag tggaagctct cccaagcgag aaacgcttgg attcagatgg
    18781 caatcgtcta tccgaatcag aactgtataa caatgcggtc gatagtgtac aaacgagtca
    18841 actaaactat acagaggatg aaaagaaaga aatctatctt tttagtcagt taaatgctgt
    18901 tggaaacttt gcgacaggaa ccattgcgac agatcctcta aatgattctc aggtggctgt
    18961 tattgcctct atttcaaagg agatgcctgg cattagtatt tctacttctt gggatagaaa
    19021 ggttttggaa acttcccttt cttctatagt tgggagtgta tccagtgaaa aagctggtct
    19081 cccagcggaa gaagcagaag cctatcttaa aaaaggctat tctctaaatg atcgtgtagg
    19141 aacctcctat ttggaaaagc aatatgaaga gaccttacaa ggaaaacgct cggtaaaaga
    19201 aatccatctg gataaatatg gcaatatgga aagcgtggat acaattgagg aaggtagtaa
    19261 gggaaacaat atcaaactga ccattgattt ggccttccaa gatagcgtgg atgctttact
    19321 gaaaagttat ttcaattccg agctagaaaa tggtggagcc aagtattctg aaggtgtcta
    19381 tgcagtcgcc cttaacccaa aaacaggtgc ggttttgtct atgtcaggga ttaaacatga
    19441 cttgaaaacg ggagagttga cgcctgattc cttgggaacg gtaaccaatg tctttgttcc
    19501 aggttcggtt gtcaaggcgg cgaccatcag ctcaggttgg gaaaatggag tcttgtcagg
    19561 aaaccagacc ttgacagacc agtccattgt cttccaaggt tcagctccca tcaattcttg
    19621 gtatactcag gcttacggtt cattccctat cacagcggtc caagctctgg agtattcatc
    19681 aaatacctat atggtccaaa cagccttagg tcttatgggg caaacctatc aacccaatat
    19741 gtttgtcggc accagcaatc tagagtctgc tatggagaaa ctgcgttcaa cctttggcga
    19801 atatggcttg ggtactgcga caggaattga cctaccagat gaatctactg gatttgttcc
    19861 caaagagtat agctttgcta attacattac taatgccttt gggcagtttg ataactatac
    19921 gccgatgcag ttggctcagt atgtagcaac tattgcaaat aatggtgttc gtgtggctcc
    19981 tcgtattgtt gaaggcattt atggtaataa tgataaggga ggactgggtg acttgattca
    20041 gcaactgcaa ccgacagaga tgaataaggt caatatatcc gactccgata tgagcatctt
    20101 gcaccaaggt ttttatcagg ttgcccatgg tactagtgga ttgacaactg gacgtgcctt
    20161 ttcaaatggc gccttggtat ccattagcgg aaaaacaggt acagccgaaa gctatgtggc
    20221 agatggtcag caagcaacca ataccaatgc ggtggcctat gccccatctg ataatcccca
    20281 aatcgctgtt gcagtggtct ttcctcataa taccaatcta acaaatggtg taggaccttc
    20341 cattgcgcgt gacattatca atctgtatca aaaataccat ccaatgaact agaaaggaaa
    20401 ttatgcttta tccaacacct attgccaagt tgattgacag ttattctaag ttaccaggta
    20461 tcgggattaa gacggctacg cgtctggcct tttatacgat tgggatgtct gctgatgatg
    20521 tcaatgaatt tgcaaaaaat ctcctttctg ctaagagaga attgacatat tgttctattt
    20581 gtggacgttt gacagacgac gatccttgtt ctatctgtac tgatccgact cgtgaccaga
    20641 caacaatttt agttcttgag gatagtagag atgtggcagc catggaaaat atccaagaat
    20701 accatggact ctatcatgtc cttcatggcc tcatttctcc tatgaatggt atcagtccgg
    20761 acgatatcaa tctcaagagc cttatgactc gtcttatgga tagtgaagtt tcagaagtga
    20821 ttgtggcgac taatgctaca gcggatggtg aagcgacttc catgtatctt tcacgtttgc
    20881 tcaagccggc tggtatcaag gttacgcgtc tagcacgagg tctcgctgtg ggagcggaca
    20941 ttgagtatgc ggacgaagtg acactcttac gagccattga aaatcggaca gagttgtaag
    21001 tgtaggcaaa tttacgaact cccttcattt ataaaaaatc aaagaggctg aaaatcgttc
    21061 ctatcggcct ctttttgtat agtgtgatga gtaggatcag gttcaagttt taaaaaacca
    21121 agcaaatatg atatactaaa gagcgagtat tctagtagaa ttaggacaaa taatatgaaa
    21181 caaacgatta ttcttttata tggtggacgg agtgcggaac gcgaagtctc tgtcctttca
    21241 gctgagagtg tcatgcgtgc ggtcaattac gaccgtttca cagtcaagac tttctttatc
    21301 agtcagtcag gtgactttat caaaacacag gaatttagtc atgctccggg gcaagaagac
    21361 cgtctcatga ccaatgaaac cattgattgg gataagaaag ttgcaccaag tgctatctac
    21421 gaagaaggtg cagtggtctt tccagtcctt cacgggccaa tgggagaaga tggctctgtt
    21481 caaggattct tggaagtttt gaaaatgcct tacgttggtt gcaacatttt gtcatcaagt
    21541 cttgccatgg ataaaatcac gactaagcgt gttctggaat ctgctggtat tgcccaagtt
    21601 ccttatgtgg ctatcgttga aggcgatgat gtgactgcta aaatcgctga agtggaagaa
    21661 aaattggctt atccagtctt cactaagccg tcaaacatgg ggtctagtgt cggtatttct
    21721 aagtctgaaa accaagaaga actccgtcca gccttagaac ttgccttccg atatgatagc
    21781 cgtgtcttgg ttgagcaagg agtgaatgcc cgtgaaattg aggttggcct cttgggtaac
    21841 tacgatgtca agagcacgct accaggagaa gttgtcaagg atgttgcctt ttatgactac
    21901 gatgccaagt atattgataa caagattact atggatattc ctgccaaaat cagtgatgat
    21961 gtggtggctg tcatgcgtca aaatgcagaa acagccttcc gtgccattgg tggccttggt
    22021 ctatctcgtt gcgatttctt ctatacagat aagggagaga tttttctcaa cgagctcaat
    22081 accatgccag gtttcaccca gtggtctatg tacccactac tttgggacaa tatggggatc
    22141 agctacccag aactaatcga gcgtttggtt gaccttgcca aggaaagttt tgacaagcgc
    22201 gaagcgcatt tgatataaaa tgaaagagag ggtagaagcc agaaccatca ctgcaaggtg
    22261 actagagttc tcggacttca gcccttttta aaggagtaga aatgaaatta acaatccatg
    22321 aaattgccca agttgttgga gtcaaaaatg atatcagtat ctttgaggac acccagttag
    22381 aaaaagctga gtttgatagt cgtttgattg gaactggaga tttatttgtg ccacttaaag
    22441 gtgcgcgtga tggccatgac tttattgaaa cagcctttga aaatggtgca gcagtaacct
    22501 tgtctgagaa agaggtctca aatcatcctt acattctagt agatgatgtt ttgacagcct
    22561 ttcaatccct agcatcctac tatcttgaaa aaacgactgt tgatgtcttt gctgttacag
    22621 gttcaaatgg caagacaacg actaaggata tgttggcgca tttactgtca acaagataca
    22681 agacctacaa aacacaagga aattacaata acgagattgg ccttccttac acagttcttc
    22741 atatgcctga agggacagaa aagttggtct tggagatggg gcaggatcac ttgggagata
    22801 ttcatctctt gtctgaattg gctcgtccaa aaacagccat cgtgaccttg gttggagaag
    22861 ctcatttggc ctttttcaaa gaccgttctg agattgctaa gggaaaaatg caaattgcag
    22921 acggaatggc ttcaggttcc ttgcttttag cgccggctga ccctatcgta gaggactatt
    22981 taccaactga taaaaaggtg gttcgttttg ggcaaggggc agagctggaa attactgact
    23041 tggttgagcg caaagatagt ctaaccttca aggccaattt cttagagcaa gcccttgatt
    23101 tgccagtaac tggcaagtac aatgcgacaa atgctatgat tgcatcctat gttgccttgc
    23161 aagaaggagt ttcagaggag caaattcgtt tggccttcca agatcttgaa ttgacgcgta
    23221 accgtaccga atggaagaaa gcagccaatg gagcagatat cctgtctgat gtatacaatg
    23281 ccaatccaac tgctatgaag ttgattttag agactttctc tgccattcca gccaatgaag
    23341 gtggcaagaa aattgcggtt ttagcggaca tgaaggaact gggtaaccag tcagttcaac
    23401 tccataatca gatgattttg agcctttctc cagatgtgct tgataccgtt attttctatg
    23461 gagaagatat tgctgaatta gcccaattgg ctagtcaaat gttcccaata ggccacgttt
    23521 actacttcaa gaaaacagaa gaccaagacc aatttgaaga cctagtcaag caggtcaaag
    23581 aaagccttgg agcctatgac caaatcttgc taaaaggttc taactctatg aatctagcca
    23641 agttggtaga aagtttagaa aatgaagaca agtgattttg tcaagtattt gcaaagaatg
    23701 attgccatta cagatactgg cttaaccttt acaaaagatc cgtttgaccg tgagcgctac
    23761 gaagacttgc gagacctttt atctgaaatg ttgaatcagg gatcagacct tgattccgaa
    23821 gaagtggcag aagtcttgaa gccaacttct gcttatgcga ctccgttaat ggacgtccgt
    23881 gcttggattg ttgaggacga aaagatttgt ctggttaggg gacaaggaga ggatagttgg
    23941 gctttgccag gtggctttgg tgaggttggc tattctccaa cagaaaatat tctcaaggaa
    24001 attgaagaag aaaccggttt taaagccaaa gttgaaagac tgctagctgt ttttgatacc
    24061 aatcgtttcc aactacagag caaacagtat acaaagtttg tttttggatg taagcttctt
    24121 gatggacaat tccaagaaaa tcaagaaatt gctgaccttc aattttttgc cattgaccaa
    24181 ctgccgaact tatctgaaaa acgcattacc aaggagcaaa tagagcttct ttggcaggtt
    24241 tatcaaggtc atagggggca atatcttgac taagaagatg attatcgtat ttctaaatcc
    24301 atttttaaca actagcatgg tataataata tgcaggaaaa ttttgaatta tgaggaagac
    24361 tagatgaatt tatgggatat tttctttacg actcaggcaa ccgagccgcc caaatttgac
    24421 cttttttggt atgttagcct atttacgctc ttagccttaa ctttttatac agcccatcgc
    24481 tatcgtgaaa agaaggttta ccaacgattt ttccaaatct tgcagactgt tcagttaatc
    24541 cttctttatg gttggtactg ggttaatcat atgccactgt cagaaagcct acccttttac
    24601 cattgccgta tggctatgtt tgtggtactc ttgcttcctg gtcagtccaa atataaacaa
    24661 tactttgcat tattgggaac atttgggaca ttagcagcct ttgtttatcc agtgccagat
    24721 gcttaccctt ttccacatat taccatttta tcctttatct ttggtcattt agcactcttg
    24781 gggaactctc tagtttatct attgagacag tataatgcgc gattgctgga tgtgaaggga
    24841 atttttctca tgacctttgc cctaaatgcc ttgatttttg tggtcaattt ggtgacaggt
    24901 ggcgattacg gatttttgac aaaaccgcca ttggttgggg atcacggtct agtagctaat
    24961 tatttacttg tttcaattgt gctggtagct actatcagtt tgactaagaa aatcttagaa
    25021 ttctttttag ctcaagaagc agaaaaaatg attgcaaagg aagcttaaca cagagctttc
    25081 ttttttgctc ttagagagtt tttacaagca gcttataaaa taagaatttc tgaatagaca
    25141 aactcaaaaa atggctggga aatttaggaa aaaagcaagc acgattaaat tttttgtgtt
    25201 ataatatttt gtgaatagct atgcctatgt ttagctatgg aataatacga agtgcgaaac
    25261 ttggaagata gagaggaagc gatgtaatgg ctagagaagg cttttttaca ggtctagata
    25321 ttggaacaag ctctgtcaag gtgcttgtgg ccgagcagag aaatggtgaa ttaaatgtaa
    25381 ttggcgtgag taatgccaaa agtaaaggtg taaaggatgg aattattgtt gatattgatg
    25441 cagcagcaac tgctatcaag tcagccattt cccaagcgga agaaaaggca ggcatttcga
    25501 ttaaatcagt gaatgtcggc ttgcctggta atcttttgca ggtagaacca actcagggga
    25561 tgattccagt aacatctgat actaaggaaa ttacggatca agatgttgaa aatgttgtca
    25621 aatcagcttt gacaaagagt atgacacctg accgtgaagt cattaccttt attcctgaag
    25681 aatttattgt ggatggtttc caagggattc gtgacccacg tggcatgatg ggggttcgcc
    25741 ttgaaatgcg tggtttgctt tatacaggac ctcgtactat cttgcacaat ttgcgtaaga
    25801 cggttgagcg tgcaggtgtt caggttgaaa atgttatcat ttcaccacta gcaatggttc
    25861 agtctgtttt gaacgaaggg gaacgtgaat ttggtgctac agtgattgat atgggggcag
    25921 gtcaaacgac tgtcgctaca atccgtaatc aagaactcca gttcacacat attctccaag
    25981 aaggtggaga ttatgtaact aaagatatct ccaaggtttt gaaaacctct cgcaaattag
    26041 cggaaggctt gaaactgaat tacggggaaa cctatccgcc tcttgcaagc aaagaaacct
    26101 tccaagtaga agttattgga gaagtagaag cagtcgaagt gacggaagcc tacttgtcag
    26161 aaattatttc tgcacgaatc aagcacatcc ttgaacaaat caagcaagaa ttagatagaa
    26221 ggcgtctatt ggacctccct ggtggtattg tcttaatcgg tgggaatgcc attttaccag
    26281 gtatggttga gcttgctcag gaagtctttg gcgtccgtgt caagctttat gttccaaatc
    26341 aagttggtat ccgtaatcca gcctttgcgc atgtgattag tttatcagaa tttgcgggtc
    26401 aattaacaga agttaatctt ttggctcagg gagcgataaa aggtgagaat gacttaagtc
    26461 atcagccaat tagttttggt gggatgctgc aaaaaacagc tcagtttgta caatcaacgc
    26521 ctgttcaacc agctcctgct ccagaagtag agccggtggc gcctacagaa ccaatggcgg
    26581 atttccaaca agcttcacaa aataaaccga aattagcaga tcgtttccgt ggcttgatcg
    26641 gaagcatgtt tgacgaataa agaggaaaaa taaattatga cattttcatt tgatacagct
    26701 gctgctcaag gggcagtgat taaagtaatt ggtgtcggtg gaggtggtgg caatgccatc
    26761 aaccgtatgg tcgacgaagg tgttacaggc gtagaattta tcgcagcaaa cacagatgta
    26821 caagcattga gtagtacaaa agctgagact gttattcagt tgggacctaa attgactcgt
    26881 ggtttgggtg caggaggtca acctgaggtt ggtcgtaaag ccgctgaaga aagcgaagaa
    26941 acactgacgg aagctattag tggtgccgat atggtcttca tcactgctgg tatgggagga
    27001 ggctctggaa ctggagctgc tcctgttatt gctcgtatcg ccaaagattt aggtgcgctt
    27061 acagttggtg ttgtaacacg tccctttggt tttgaaggaa gtaagcgtgg acaatttgct
    27121 gtagaaggaa tcaatcaact tcgtgagcat gtagacactc tattgattat ctcaaacaac
    27181 aatttgcttg aaattgttga taagaaaaca ccgcttttgg aggctcttag cgaagcggat
    27241 aacgttcttc gtcaaggtgt tcaagggatt accgatttga ttaccaatcc aggattgatt
    27301 aaccttgact ttgccgatgt gaaaacggta atggcaaaca aagggaatgc tcttatgggt
    27361 attggtatcg gtagtggaga agaacgtgtg gtagaagcgg cacgtaaggc aatctattca
    27421 ccacttcttg aaacaactat tgacggtgct gaggatgtta tcgtcaacgt tactggtggt
    27481 cttgacttaa ccttgattga ggcagaagag gcttcacaaa ttgtgaacca ggcagcaggt
    27541 caaggagtga acatctggct cggtacttca attgatgaaa gtatgcgtga tgaaattcgt
    27601 gtaacagttg tcgcaacggg tgttcgtcaa gaccgcgtag aaaaggttgt ggctccacaa
    27661 gctagaactg ctactaacta ccgtgagaca gtgaaaccag ctcattcaca tggctttgat
    27721 cgtcattttg atatggcaga aacagttgaa ttgccaaaac aaaatccacg tcgtttggaa
    27781 ccaactcagg catctgcttt tggtgattgg gatcttcgcc gtgaatcgat tgttcgtaca
    27841 acagattcag tcgtttctcc agtcgagcgc tttgaagccc caatttcaca agatgaagat
    27901 gaattggata cacctccatt tttcaaaaat cgttaagtaa atgaatgtaa aagaaaatac
    27961 agaacttgtt tttcgagaag ttgcagaggc tagtctgagt gctcatcgag agagtggttc
    28021 ggtctctgtc attgcagtta ccaagtatgt agatgtaccg acagcggaag ccttgcttcc
    28081 gctaggtgtt catcatatcg gtgaaaatcg tgtagataag tttctggaaa aatatgaagc
    28141 tttaaaagat cgagatgtga cttggcattt gattggtacc ttgcaaagac gtaaggtgaa
    28201 agatatcatt caatacgttg attatttcca tgcattggac tcagtaaagc tagcagggga
    28261 aattcaaaaa agaagtgacc gagtcatcaa gtgtttcctt caagtaaata tttctaaaga
    28321 agaaagcaaa cacggttttt cgagagagga actgctggaa atcttgccag agttagccag
    28381 actagataag attgaatatg ttggtttaat gacgatggca ccttttgagg ctagcagtga
    28441 gcagttgaaa gagattttca aggcggccca agatttacaa agagaaattc aagagaaaca
    28501 aattccaaat atgcctatga ccgagttaag tatgggaatg agtcgtgatt ataaagaagc
    28561 gattcaattc ggttccactt ttgttcgtat aggtacatca ttttttaagt aggagagaac
    28621 catgtcttta aaagatagat tcgatagatt tatagattat tttacggagg atgaggattc
    28681 aagtctccct tatgaaaaaa gagatgagcc tgtgtttact tcagtaaatt cttcacagga
    28741 accggctctc ccaatgaatc aaccttcaca gtcggctggt acaaaagaga acaatatcac
    28801 cagacttcat gcaagacaac aggaattggc aaatcagagt cagcgtgcaa cggataaggt
    28861 cattatagat gttcgttatc ctagaaaata tgaggatgca acagaaattg ttgatttatt
    28921 ggcaggaaac gaaagtatct tgattgattt tcagtatatg acagaggtgc aggctcgtcg
    28981 ttgtttggac tatttggatg gagcttgtca tgttttagct ggaaatttga aaaaggtagc
    29041 ttctaccatg tatttgttga caccagtgaa cgttattgta aatgttgaag atatccgttt
    29101 accagatgaa gatcaacagg gtgagttcgg ttttgatatg aagcgaaata gagtacgata
    29161 atgatttttt taattcgtat gatttataat gcagtggata tttactccct gattttggta
    29221 gccttcgctg tcatgtcttg gtttccaggt gcctacgaat ccagtttagg tcgttggatt
    29281 gtagcgttgg tgaaaccagt gcttgctccc ttgcaacgcc tgcctttaca gatagcgggt
    29341 cttgatttat ctgtttgggt cgcgattgtt ttggttcgat ttttaggaga aaacctagtg
    29401 cgttttctgg cgatgatagg atgaataaag ggatttatca gcatttctcc atagaagatc
    29461 gtccatttct tgacaaggga atggaatgga taaagaaggt agaagatagc tatgctcctt
    29521 ttttaactcc ttttatcaat cctcatcagg agaagctatt aaagattttg gccaaaacct
    29581 atggtattgc ttgtagcagt agtggggaat tcgtctcgag tgagtatgtt cgagttttat
    29641 tatacccaga ttatttccaa ccagagtttt cagattttga aatatctctc caggaaattg
    29701 tgtattccaa taaatttgaa catttaacgc atgctaagat tttagggaca gtcatcaatc
    29761 aattagggat tgaacggaaa ctttttggag atatcctagt agatgaagaa cgggcgcaga
    29821 ttatgattaa tcagcagttt cttcttctct ttcaagatgg actaaagaaa attggtcgta
    29881 tacctgtttc gctggaggaa cgtcctttca ccgagaaaat agataagcta gaacagtatc
    29941 gagaactgaa tttatctgtg tctagttttc gattagatgt tcttttatca aatgttttga
    30001 aactatctag gaatcaagca aaccagttga ttgaaaagaa acttgtccaa gtaaattatc
    30061 atgtggtaga caaatcagat tacactgttc aagttggaga cttgattagt gtgagaaaat
    30121 ttggtcgctt gagattattt caagataagg gacaaacgaa aaaagagaag aaaaaaataa
    30181 ccgtccagtt attattaagt aagtgaggaa tagaatgcca attacatcat tagaaataaa
    30241 ggacaagacc tttggaactc gattcagagg ttttgatcca gaagaagtcg atgaattttt
    30301 agatattgtg gttcgtgatt acgaagatct tgtgcgtgcg aatcatgata aaaatttgcg
    30361 tattaagagt ttagaagagc gtttgtctta ctttgatgaa ataaaagatt cattgagcca
    30421 gtctgtattg attgcccagg atacagctga gagagtgaaa caggcggcgc atgaacgttc
    30481 aaacaatatc attcatcaag cagagcaaga tgcgcaacgc ttgttggaag aagctaaata
    30541 taaggcaaac gagattcttc gtcaagcaac tgataatgct aagaaagtcg ctgttgaaac
    30601 agaagaattg aagaacaaga gccgtgtctt ccaccaacgt ctcaaatcta caattgagag
    30661 tcagttggct attgttgaat cttcagattg ggaagatatt ctccgtccaa cagctactta
    30721 tcttcaaact agtgatgaag cctttaaaga agtggttagc gaagtacttg gagaaccgat
    30781 tccagctcca attgaagaag aaccaattga tatgacacgt cagttctccc aagcagaaat
    30841 ggaagaatta caagctcgta ttgaggtagc cgataaagaa ttgtctgaat ttgaagctca
    30901 gattaaacag gaagtggaaa ctccaactcc tgtagtgagt cctcaagttg aagaagagcc
    30961 tctgctcatc cagttggccc aatgtatgaa gaaccagaag tagctccagt gcatccgaca
    31021 ggtccaacac cagctacaga aactgttgat tcagcaccgg gatttgaagc accgcaagaa
    31081 tctgttacaa ttttataaga aatattctga gaacaatatc ttatccttat attcccagcg
    31141 agcaggagat ggtgtgagtc ctgcattccc tatcgataag attatcctct caaaaactca
    31201 agtctgaatc tagtaagatt tgacgttccc cacgttacgg gataagaggg agaaagacta
    31261 aaactttttc cgaataaagg tggtaccacg attttcgtcc tttttggaag tcgtggtttt
    31321 taatttgtta ttatttataa aggagatacc atgaaactca aagacaccct taatcttggg
    31381 aaaactgaat tcccaatgcg tgcaggcctt cctaccaaag agccagtttg gcaaaaggaa
    31441 tgggaagatg caaaacttta tcaacgtcgt caagaattga accaaggaaa acctcatttc
    31501 accttgcatg atggccctcc atacgctaac ggaaatatcc acgttggaca tgctatgaac
    31561 aagatttcaa aagatatcat tgttcgttct aagtctatgt caggatttta cgcaccattt
    31621 attcctggtt gggatactca tggtctgcca atcgagcaag tattgtcaaa acaaggtgtc
    31681 aaacgtaaag aaatggactt ggttgagtac ttgaaacttt gccgtgagta cgctctttct
    31741 caagtagata aacaacgtga agattttaaa cgtttgggtg tttctggtga ctgggaaaat
    31801 ccatatgtga ccttgactcc tgactatgaa gcagctcaaa ttcgtgtatt tggtgagatg
    31861 gctaataagg gttatatcta ccgtggtgcc aagccagttt actggtcatg gtcatctgag
    31921 tcagcccttg ctgaagcaga gattgaatac catgacttgg tttcaacttc cctttactat
    31981 gccaacaagg taaaagatgg caaaggagtt ctagatacag atacttatat cgttgtctgg
    32041 acaacgactc catttaccat cacagcttct cgtggtttga cggttggtgc agatattgat
    32101 tacgttttgg ttcaacctgc tggtgaagct cgtaagtttg tcgttgctgc tgaattattg
    32161 actagcttgt ctgagaaatt tggctgggct gatgttcaag ttttggaaac ttaccgtggc
    32221 caagaactca accacatcgt aacagaacac ccatgggata cagctgtaga agagttggta
    32281 attcttggtg accacgttac gactgactct ggtacaggta ttgtccatac agcccctggt
    32341 tttggtgagg acgattacaa tgttggtatt gctaataatc ttgaagtcgc agtgactgtt
    32401 gatgaacgtg gtatcatgat gaagaatgct ggtcctgaat ttgaaggtca attctatgaa
    32461 aaggtagttc caactgttat tgaaaaactt ggtaacctcc ttcttgccca agaagaaatc
    32521 tctcactcat atccatttga ctggcgtact aagaaaccaa tcatctggcg tgcagttcca
    32581 caatggtttg cctcagtttc taaattccgt caagaaatct tggacgaaat tgaaaaagtg
    32641 aaattccact cagaatgggg taaagtccgt ctttacaata tgatccgtga ccgtggtgac
    32701 tgggttatct ctcgtcaacg tgcttggggt gttccacttc ctatcttcta cgctgaagat
    32761 ggtacagcta tcatggtagc tgaaactatt gaacacgtag ctcaactttt tgaagaacat
    32821 ggttcaagca tttggtggga acgtgatgcc aaagacctct tgccagaagg atttactcat
    32881 ccaggttcac caaacggcga gttcaaaaaa gaaactgata tcatggacgt ttggtttgac
    32941 tcaggttcat catggaatgg agtggtggta aaccgtcctg aattgactta cccagccgac
    33001 ctttacctag aaggttctga ccaataccgt ggttggttta actcatcact tatcacatct
    33061 gttgccaacc atggcgtagc accttacaaa caaatcttgt cacaaggttt tgcccttgat
    33121 ggtaaaggtg agaagatgtc taaatctctt ggaaatacta ttgctccaag cgatgttgaa
    33181 aaacaattcg gtgctgaaat cttgcgtctc tgggtaacaa gtgttgactc aagcaatgac
    33241 gtgcgtatct ctatggatat cttgagccaa gtttctgaaa cttaccgtaa gattcgtaac
    33301 actcttcgtt tcttgattgc caatacatct gactttaacc cagctcaaga tacagtcgct
    33361 tacgatgagc ttcgttcagt tgataagtac atgacgattc gctttaacca gcttgtcaag
    33421 accattcgtg atgcctatgc agactttgaa ttcttgacga tctacaaggc cttggtgaac
    33481 tttatcaacg ttgacttgtc agccttctac cttgattttg ccaaagatgt tgtttacatt
    33541 gaaggtgcca aatcactgga acgccgtcaa atgcagactg tcttctatga cattcttgtc
    33601 aaaatcacca aactcttgac accaatcctt cctcacactg cggaagaaat ttggtcatat
    33661 cttgagtttg aagccgaaga cttcgtccaa ttgtcagaat taccagaggc tcaaacttct
    33721 gctaaccaag aagaaatctt ggatacatgg gcagccttca tggacttccg tggacaagca
    33781 caaaaagcct tggaagaagc tcgtaatgca aaagttatcg gtaaatcact tgaagcacac
    33841 ttgacagttt atccaaatga agtggtgaaa actctactcg aagcagtaaa cagcaatgta
    33901 gctcaacttt tgatcgtgtc tgagttgacc atcgcagaag aaccaactcc ggaagctgcc
    33961 cttagcttcg aagatgtagc cttcacagtt gaacgtgctg ctggtgaagt atgtgaccgt
    34021 tgccgtcgta tcgacccaac aacagcagaa cgcagctacc aggcagttat ctgtgaccac
    34081 tgtgcaagca tcgtagaaga aaattttgcg gatgcagtcg cagaaggatt tgaagagaaa
    34141 taagattgaa aagtctaggc aaaattcaat ttgagaagaa aagacaacta attttatagt
    34201 ctattaaacg cattgtatca cgtttttgaa cacctgatat gatgcgtttt tatttatttt
    34261 gaaaatttgt gaggtgtgac tttttatact caacaagaat caaagagaaa cttagcaagc
    34321 taacagtagt aagataaaat aggaatttga tattagggat aagattggta aatagtgtaa
    34381 tatttttaca acaataaat
//
LOCUS       DAVHIT010000013        34192 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVHIT010000013 DAVHIT010000000
VERSION     DAVHIT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.013"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(111..1745)
                     /locus_tag="VVN16_000498"
     CDS             complement(111..1745)
                     /locus_tag="VVN16_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571941.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTTVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENLDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1726..2475)
                     /locus_tag="VVN16_000499"
     CDS             complement(1726..2475)
                     /locus_tag="VVN16_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571942.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2654..4108
                     /locus_tag="VVN16_000500"
     CDS             2654..4108
                     /locus_tag="VVN16_000500"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU5571943.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4198..6816)
                     /gene="alaS"
                     /locus_tag="VVN16_000501"
     CDS             complement(4198..6816)
                     /gene="alaS"
                     /locus_tag="VVN16_000501"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5571944.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNHNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6838..7323)
                     /locus_tag="VVN16_000502"
     CDS             complement(6838..7323)
                     /locus_tag="VVN16_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU5571945.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7598..7837)
                     /locus_tag="VVN16_000503"
     CDS             complement(7598..7837)
                     /locus_tag="VVN16_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571946.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8098..9168)
                     /locus_tag="VVN16_000504"
     CDS             complement(8098..9168)
                     /locus_tag="VVN16_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5571947.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVAPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9165..9938)
                     /locus_tag="VVN16_000505"
     CDS             complement(9165..9938)
                     /locus_tag="VVN16_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5571948.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9935..10741)
                     /locus_tag="VVN16_000506"
     CDS             complement(9935..10741)
                     /locus_tag="VVN16_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5571949.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10722..11879)
                     /locus_tag="VVN16_000507"
     CDS             complement(10722..11879)
                     /locus_tag="VVN16_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5571950.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12039..12944)
                     /gene="murB"
                     /locus_tag="VVN16_000508"
     CDS             complement(12039..12944)
                     /gene="murB"
                     /locus_tag="VVN16_000508"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5571951.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13056..13808)
                     /locus_tag="VVN16_000509"
     CDS             complement(13056..13808)
                     /locus_tag="VVN16_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU5571952.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALEIVLVAYGTKEIFCKKS"
     gene            complement(13818..14549)
                     /gene="budA"
                     /locus_tag="VVN16_000510"
     CDS             complement(13818..14549)
                     /gene="budA"
                     /locus_tag="VVN16_000510"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU5571953.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGTGEQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     VGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14691..15962
                     /locus_tag="VVN16_000511"
     CDS             14691..15962
                     /locus_tag="VVN16_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU5571954.1"
                     /translation="MKRQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFMAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15982..16797)
                     /locus_tag="VVN16_000512"
     CDS             complement(15982..16797)
                     /locus_tag="VVN16_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5571955.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16954..17607)
                     /gene="phoU"
                     /locus_tag="VVN16_000513"
     CDS             complement(16954..17607)
                     /gene="phoU"
                     /locus_tag="VVN16_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5571956.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17619..18377)
                     /gene="pstB"
                     /locus_tag="VVN16_000514"
     CDS             complement(17619..18377)
                     /gene="pstB"
                     /locus_tag="VVN16_000514"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5571957.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18390..19193)
                     /gene="pstB"
                     /locus_tag="VVN16_000515"
     CDS             complement(18390..19193)
                     /gene="pstB"
                     /locus_tag="VVN16_000515"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5571958.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19204..20088)
                     /gene="pstA"
                     /locus_tag="VVN16_000516"
     CDS             complement(19204..20088)
                     /gene="pstA"
                     /locus_tag="VVN16_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5571959.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20078..20995)
                     /gene="pstC"
                     /locus_tag="VVN16_000517"
     CDS             complement(20078..20995)
                     /gene="pstC"
                     /locus_tag="VVN16_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5571960.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20995..21873)
                     /gene="pstS"
                     /locus_tag="VVN16_000518"
     CDS             complement(20995..21873)
                     /gene="pstS"
                     /locus_tag="VVN16_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU5571961.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22447..23751)
                     /locus_tag="VVN16_000519"
     CDS             complement(22447..23751)
                     /locus_tag="VVN16_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU5571962.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23752..24525)
                     /locus_tag="VVN16_000520"
     CDS             complement(23752..24525)
                     /locus_tag="VVN16_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5571963.1"
                     /translation="MESKFEFAKGLVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24515..24793)
                     /locus_tag="VVN16_000521"
     CDS             complement(24515..24793)
                     /locus_tag="VVN16_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU5571964.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24793..25194)
                     /locus_tag="VVN16_000522"
     CDS             complement(24793..25194)
                     /locus_tag="VVN16_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU5571965.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25404..26021)
                     /locus_tag="VVN16_000523"
     CDS             complement(25404..26021)
                     /locus_tag="VVN16_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU5571966.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETVQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(26011..26784)
                     /locus_tag="VVN16_000524"
     CDS             complement(26011..26784)
                     /locus_tag="VVN16_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU5571967.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26795..27532)
                     /locus_tag="VVN16_000525"
     CDS             complement(26795..27532)
                     /locus_tag="VVN16_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU5571968.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27537..28667)
                     /locus_tag="VVN16_000526"
     CDS             complement(27537..28667)
                     /locus_tag="VVN16_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU5571969.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28751..29140)
                     /locus_tag="VVN16_000527"
     CDS             complement(28751..29140)
                     /locus_tag="VVN16_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5571970.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29156..29539)
                     /locus_tag="VVN16_000528"
     CDS             complement(29156..29539)
                     /locus_tag="VVN16_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU5571971.1"
                     /translation="MLEVAYILVALAVIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29540..30328)
                     /locus_tag="VVN16_000529"
     CDS             complement(29540..30328)
                     /locus_tag="VVN16_000529"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU5571972.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30321..31256)
                     /locus_tag="VVN16_000530"
     CDS             complement(30321..31256)
                     /locus_tag="VVN16_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU5571973.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31386..31562)
                     /locus_tag="VVN16_000531"
     CDS             complement(31386..31562)
                     /locus_tag="VVN16_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU5571974.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31702..32409)
                     /locus_tag="VVN16_000532"
     CDS             complement(31702..32409)
                     /locus_tag="VVN16_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU5571975.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32560..33588
                     /gene="queA"
                     /locus_tag="VVN16_000533"
     CDS             32560..33588
                     /gene="queA"
                     /locus_tag="VVN16_000533"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU5571976.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
BASE COUNT        10216 a         7819 c         6537 g         9620 t
ORIGIN      
        1 acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca ttctaacaaa
       61 ctataaaagc gtttgtcgaa ttgaatttat caagcaagca accaactatc tcatcttttt
      121 tctatttctg ccaatatgcg tgacaggtaa taatgatagc caaaaatagc aagagcaagc
      181 aagacgataa gagctcctac tcccaagttg atggcaagga taggggagag agactgaacc
      241 aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa caataaaact
      301 atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca attggttgac
      361 agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc aatgatccag
      421 actacaagaa ggtaaatcat cgaaaggatg gacaagccta gatatagaga aagaccgagc
      481 aaagtcagaa ttggtaaaaa ggactgaaca gcaaagataa tccaaaattt cactttcaca
      541 taacgagcaa agtcaaaggg taaactcttc agaaaatcaa gattttccct ctccaaggac
      601 aaggcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa cactcctaca
      661 aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga atctcggatt
      721 ttggaaatca gactgatcat catgagataa ggaaggaaag cacttgtaaa aagcactgta
      781 atcacgccag tcccctgtcc caagagggtg aggtggtagc gtaaaaccat acggaaaaag
      841 ccctttttag tagttgaaat cctctccttg ctgcgacgtt cttttttgac cttctcctca
      901 ctgttaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt caggtaaagc
      961 aagaaaacag ttaaagctat ccaagcgagc agacccaata tggcttctat tgaaaaaggc
     1021 tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat gtctctaact
     1081 ttgtcaacaa tacttccaaa agtcgactgt agaaagaaga caaatattaa aggtatgaga
     1141 actcctatcc caatcatcac attcgaaaaa atagactgat actttctgaa gaccgtagtt
     1201 tgagccaaga aatgcactgc tactaccatc actagagcca cagagacaaa taataaggtc
     1261 aaggacagta gcatcaaagg caaacccagc catagagaag gagctagcct aatgtagagg
     1321 actagaaaat aagctaggat tggtacaatt ccagttagag ctggcaaaag gacagacagt
     1381 cctttagcaa ttataatctc tgattcttta aaggcatagg gcctatacga taccaaatcc
     1441 ttactctcat aaaagacatt gtaaaaggcc gttaaagaag ttgaaaaggc aatcactagt
     1501 aaaatagcaa ccatcgagct aaaataaata ggtatttcct caaaaggaaa atgaatggct
     1561 atattactaa aacagatgag catcaagaga ctggaaaaaa tgtaagaact taggactcta
     1621 gcggaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag attagccaga
     1681 ttagcttctt gagatgaata gaggatattg atatcaacta attttttaat gaccttgaga
     1741 cgcatccgca acctcctctt ttctaccagc aagactaagg tagatacttt ccaaagactg
     1801 gtctgggtgg tctttcctca agtcctctac cttaccacaa taaatcaaat gccccttttt
     1861 caaaatggca atccgatcac agacttgctc tgccacctct aggacatgag ttgaaaacaa
     1921 gactgtcttc cctttttgtg catgttcctt catcatctgt ttcaaatcaa aggcagcctg
     1981 gggatccaaa ccagtcaagg gttcatccaa gacccaaata tcgggatcag acaagagtgc
     2041 tccgatgaca aagactttct gacgcattcc gtgagaaaga gtttcaataa cctgatagcg
     2101 attttcagca aaatcaaaaa cgttcaatag cctagctaga ctagcctcca agtcagatct
     2161 actcagatca taggatgagg cgatcaattc ccaaaattca ttggccgtta agcgtaaaaa
     2221 taagtcaggc gagtctgcta cgtagccaat ctttcgttta atagccaagc gattttccga
     2281 taactcctga ccgtctacca aaatacgacc actgctgggt gaaatgatac tgactaggga
     2341 ttttatggtg gtcgattttc cagccccatt atgaccaatc aagcccataa tctctccatt
     2401 ttcaatctgc aaattgagat tactcaaggc ctctttatca ccatacaact tactaacatt
     2461 ttgaaattca atcatgggac aactcctatc tttatctgta ttatacatcg ctatactagc
     2521 actttgatac aaaaaaatca atactttatt ttaagtagtt aaaatagttt ccttctatct
     2581 tacgcaaacg tttgcgccaa gagatacttt atgatagaat aaagaacaag attgacaagt
     2641 aagaggaaac atcatgcaaa atcaaacact catgcaatac tttgaatggt atctgcccca
     2701 cgacggtcaa cactggacgc gtctggctga aaatgctcca cacctagctc atctggggat
     2761 cagtcacgtc tggatgccac cagccttcaa ggcaaccaac gaaaaagatg tcggctatgg
     2821 ggtctatgac ttatttgact taggagagtt caaccaaaaa gggactgtcc gcaccaagta
     2881 tggtttcaaa gaagactatc ttcaagccat tcaagccctt aaagcacagg gaattcaacc
     2941 tatggccgat gtagttctca accacaaggc tgctgccgat cacagggaag cctttcaggt
     3001 catcgaagtt gatcctgtag accgtacagt tgaacttgga gaacccttca ccatcaatgg
     3061 ctggactagt tttaccttcg atggtcgcca agatacctat aatggcttcc actggcattg
     3121 gtaccacttc actggtacag actacgatgc caaacgcagt aaatctggga tttatctgat
     3181 ccaaggggac aacaagggct gggctaacga ggaattggtc gataacgaaa acggaaacta
     3241 cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc aaaacatcta
     3301 tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt tggatgccgt
     3361 taagcatatt gactctttct ttatgcgcaa cttcatccgc gatatgaagg aaaaatacgg
     3421 tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca atctggacta
     3481 tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccacc agaatctctt
     3541 tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag atagcctggt
     3601 tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc aacgaggaca
     3661 agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc tcattttgtt
     3721 acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt caggccagta
     3781 tgctcaagaa gatttcaaag aaatccttga ccgcctccta gccatccgaa aagatttggc
     3841 ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg tacgttcagg
     3901 tgctgaaaat caatccccaa tcgcagtcct tatctcaaat gaccaagaaa acagcaagtc
     3961 aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg gtaaccacca
     4021 aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag ctagatccgt
     4081 aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag gtccaagcgg
     4141 atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt tacttgatta
     4201 caatttacct gctactgcat ccaacaattc ttggatctta gcttggttgc ttcctcctgc
     4261 catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta attctttgac
     4321 aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga ctttgtcacc
     4381 gatagcggca actaggacaa gaagatcaga gtagtctttt tgtttccagt tatctgcaaa
     4441 ggtacgaagg gcacctgcat cggatacaga cacttgacta gcaatgtaac gatgaccgtt
     4501 gacttcctta acatctttga agatatcgcc tgcggctgca gctgcggctt tttctttcaa
     4561 ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta ccttgtgagg
     4621 tacttccttg acttgaggtg ctttcaaggt tgctgcgata gctttaagag catcctcttg
     4681 ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacggc gagttcctga
     4741 accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt tgccaacatg
     4801 agtaccacca caaagttcaa tagagtagtc accgatagtc acgacacgaa cttccttgcc
     4861 gtatttctca ccaaagaggg ccatagctcc catttcttta gcagtgtcaa tatccgtttc
     4921 aactgtctta acttcaagag cttcccagat tttttcgttg acttgctgtt caatcgcacg
     4981 caattcttca gcagttacag cttggaagtg ggtaaagtca aagcgaagga attcaacttc
     5041 gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg cagcgtgaag
     5101 caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat caattgccaa
     5161 ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa gggcttgacc
     5221 atttggggct ttctgaacat tggtcacagt agccacaacc ttacctgact catccaagat
     5281 ttgtccgtgg tcagcgacct gtccacccat ttcagcatag aatggcgttt ccgcaaagat
     5341 aagtgaggta gttccttctg atacagcttc tacttctgca ttgtccgcca cgatagctac
     5401 caatttagaa gacaattggc tagcattgtg gttgaagaca ctttctacag tgatgttttg
     5461 aagagtttca ttttgcatac ccattgagcc acccttgaca gctgacgcac gcgcgcgttc
     5521 ttgttgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac cagcctcttc
     5581 agcaatttct tcagtcaatt caactgggaa tccgtatgta tcatagagtt taaagacatc
     5641 ttgaccagca atgacagatt gacctttttc tttcaagtct gccacaatgc cttgggcaaa
     5701 gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa cgattttctc
     5761 aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt ttccaacagt
     5821 tggaacaagt ttgtaaagga aaggctcgtt gatacccaat ttttgaccat gcatagaagc
     5881 acgacggaga agacgacgaa gaacataacc acgaccttca tttcctggaa gggcaccatc
     5941 accgatagca aatgaaagag aacgaatgtg gtctgcgata accttgaagc tcatgttgtc
     6001 gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa tcggcatgaa
     6061 gaggtccgtt tcaaagttgg tcttagcccc ttggataaca gccaccaaac gctccaaacc
     6121 agcgcccgta tcaatgttct tatgtggcaa ttccttgtat tcgctacgag gaacagcagg
     6181 gtctgcgtta aattgtgaca aaacgatgtt ccagatttca atataacggt cgttttcaat
     6241 atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc cacggtcaaa
     6301 gaagatttct gtatctggtc cagaaggtcc cgcaccgatt tcccagaagt tgtcctcaat
     6361 tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag aatctttatc
     6421 gtctggatag taggtcatgt aaagtttttc agcagggaaa tcaaaccatt cagggcttgt
     6481 caaaagctca taagcccaag tgatagcttc gtcacggaag taatccccga tagagaagtt
     6541 ccccagcatt tcaaacatgg tatggtgacg cgcagtcttc cctacgtttt cgatgtcgtt
     6601 ggtacggata gccttttggg cattggtaat acgtggattt tcagggataa tggtcccgtc
     6661 aaagtatttc ttaagggttg ctaccccaga gttgatccac aaaagagttg ggtcatttac
     6721 aggaaccaaa cttactgatg gttctactga gtgagctttg gtcgcccaga aatcaagcca
     6781 catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt tacttgttta
     6841 atgtgattgg ctttccagca tttccacata gtcaatcgcg acacagaggg aaatgactag
     6901 gtctgcataa gcgtcctcaa gaaccgttac ggtataggta gaggtcagat ggaagagttc
     6961 cttcttaatt tccgcaatca gctgatcgcg atcatccagc aatttgaaat tcaaatccca
     7021 gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc gccagaaggt
     7081 caacttctta cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa aacgaggaag
     7141 caaggtcaag atttctttac taatctcact gacttgttca ccagccgcat catagatggt
     7201 aaaggtttta ggaatcttaa aaaatgatcc ctccacctga taggcaattt ctcccctgtc
     7261 attcttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa gaaatgtttt
     7321 catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa aaaccgcggt
     7381 accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta tgattgagta
     7441 gcatgacttc ctagcttaga tggctcgcag caccgccatt tctctggact aagacaagtg
     7501 aaaatcaatt ctcaactttc ttattataac gtttttttaa gcttgcgtca actggaaatg
     7561 atctccgttg aattagacca attccctaca tctctgatta ctttttcagg atatattttt
     7621 tcttactgcc gtttttcttt ttatcccaaa ttttcatatt actaaacaca gctactagaa
     7681 tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt aggtattgtc
     7741 gatccaagcc atcctttaaa tggaatagta taacagtttg gttaacaatc ataaaggttg
     7801 gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc tttctgtaag
     7861 gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc tgtacttgag
     7921 tacggcaagg caacgctgac gtggtttgaa gagtataggc ttagtatact actaggcaag
     7981 caaataagca aataaacaaa taaacaacta gaatagaaaa agatagggct ctaaaaactg
     8041 acttctattc cttaaaaacg aaccagcttg actgattcgt cttcttacgt ttatctccta
     8101 cttccgatac attttaaact gtaggaagag gtcgctatat ttccctgtcc atttatggtc
     8161 aaatttctca taaacttcta ggtgtttcat ggtttcaaca tcgggataga aggccttatc
     8221 ttcctttgtt tcctctggga gcaattcctt cgctggtagg tttggtgttg aatagccgac
     8281 atactccgca ttttggagag cattttcagg tttcaacata aagttgataa aggcataggc
     8341 tgagttttgg tttttaactg ttttgggaat gaccatattg tcaaaccaaa gattgctggc
     8401 ctctgtcggt gccacataac gtagattttc atttttttct aacatttggc tggcttcacc
     8461 agagaaggtc acgccgattg caacattatt ctgaatcata tagcccttca tctcgtccgc
     8521 aacgatagcc ttgatatttg gagtcagttt gtagagctta tccactgtct cttccaactg
     8581 ctgcagatcc ttggagttga ggctgtagcc gagggaattg agtcctagtc ccagcacctc
     8641 acgcgcccca tcaaagagca tgatagaatt cttatactcc ggcttccaaa ggtcatccca
     8701 atgctcaggc gcttcatcta ccatggtttc gttgtagaca attcctaagg ttccccagaa
     8761 gtaagggatg gagaatttat tacctgggtc aaaggactgg ttgagaaact ctggtccgat
     8821 attttcgatt cctttaattt ttgaataatc aagcggaacc aagaggtctt cgtccttcat
     8881 cttgttaatc atgtattcac ttggaatggc aatatcgtag gtcgttccac cctgctttat
     8941 cttagtgtac atggcttcgt tggagtcaaa agtctcgtac tgaacttgaa ttcctgtttc
     9001 ttctgtaaac tgagtcaaga gttcaggatc gatatagtct ccccagttat agataaccaa
     9061 tttttgacta tctcgactat tgattttact atctaaatga gtcgcaattc cccacaagac
     9121 aaggataatc gctgcaattc ctgctaaaaa tgaatagatt tttttcatgc ttgctcctcc
     9181 ttctcacgag agataaagta ataacctaca actaggataa tactaaagag aaagactaga
     9241 gcagacaggg cattgatttc taaggaaatc cccttgcgag cacgagagta aatctcgact
     9301 gatagggttg aaaagccatt tcctgttaca aagaaggtca cggcaaaatc atccagcgaa
     9361 taggtgaagg ccatgaagta acctgcaatg atagacggag tcaggtaagg aagcatgatt
     9421 tccttaaaca tctgaaattg actagctccc aagtcatagg ccgcatgaat catgtcgcca
     9481 ttcatttcct tgagtcgagg caagaccatc aagaccacga taggaatgga gaaggccacg
     9541 tgactagata gaacggtcaa aaagccaagt gaaaacttga gttgggtaaa gagaatcaag
     9601 aagctagcac caatcataac gtcaggcgca accatgagga tattattgag tgatagaaag
     9661 gcttcttggt atttcttacg agactggtag atgtaaatgg caccaaaagt cccgataatg
     9721 gtcgctatca aggctgatag gaaggccaag aaaaatgtct gagccaaaat cagcatgagt
     9781 ctcccatctc caaacatggt ttcaaagtga gtccagctaa aacctgtaaa gctattcata
     9841 tcatcaccag cattaaaggc atagccaatc aagtaaaaaa tcggcaggta gaggaccaga
     9901 aagaccagtc ccagataaag gttggcaaat tttttcatcg ttctctcctt tccttggtca
     9961 cccacatggt gatgaacatg gtcaggatga gaatcacacc gatggttgaa cccataccat
    10021 agttgtcatt ggttagaaaa ttctgctcaa tagccgtccc caaggtgata acgcggttcc
    10081 caccaatcaa acgggtcagc atgaagagac tcaaacttgg gataaagacc gactgaaccc
    10141 cacttcgcac accgttcata gatagaggga agatgacatg acggaaggtc tcccacttag
    10201 ttgcaccaag gtcgtaacta gcattgatga gattattatc catatcgtct aagacattga
    10261 aaatcggcaa aatcataaag gggagctcga tgtagcttgc gacaaagata aaggaaaaat
    10321 cggtaaaaag caactgttgt gaaccaattc cgataaattc caagaattgg ttaatagagc
    10381 cattttgacc aaaaatcccg ataaaagcat aggctttaag gagcaaattg atccaggtag
    10441 gaaggataat cagcatgagc cagagttgac ggtgcttgag acgggtcaaa aagagggctg
    10501 ttggatagct gataagcaag gtcacaaagg tcacaatccc tgcataaagc actgagttga
    10561 aactcatttt gaggtaggtc aagttttgtg acgcaaagta agatttgtaa ttttctaaac
    10621 tgaactggcc ttcgatgttg aaaaaggatt gaccgaaaat caagaccaag ggtgccaata
    10681 caaagagcgc aatccaaagc atgtagggta ctacaaagag tttagagctt gttttcttca
    10741 tctctttcct cctcgattgc attgatcaaa cctgcttctt gctcttcgat ttctacgtac
    10801 tcctcaatac gagcatcgaa ctcttcttcg gtttcattga gacgcatgat gtggatgtct
    10861 tctggttcaa agtccagacc gatttcctca cccacgatag ccttacgggt tgagtggatc
    10921 atccattcat ttccaagttc gtcataggcg ataatttcat aatgaactcc acggaaaagc
    10981 tgggtatcga ccttaacttg gagcttgcct tcttcaggaa gggtaatgcg caagtcctct
    11041 ggacgaataa cgacctcaac aggttcattt ggcttcatcc caccatcaac cgcttcaaag
    11101 cgtttgccgt taaattcgac caagtagtcc tcaatcatgg tacctggcaa gatgtttgac
    11161 tccccgataa aggtggcaac aaagtggttg attggctcat cgtagatgtc cacaggggtt
    11221 ccagactgga caatctcgcc atcattcata acgaaaatcc agtcactcat ggcaagagct
    11281 tcttcctgat cgtgagtgac aaagacaaag gtaatgccca atcgttgttg taattcacgc
    11341 aattcgtact gcatgtctgt tctcaatttc aagtccagcg ctgataaagg ctcgtccaac
    11401 aagaccacac ggggttggtt gatgatagca cgggcgatgg ccacacgctg acgttgtcct
    11461 ccagaaagtt tgcggatgga acgtttttca taaccttcca actgaaccat cttgagaact
    11521 tccgctacac gctgctcgat ttctttctta tcaattttac gcaagcgaag tggaaaggca
    11581 acattttcaa acacattcat atgtgggaac aaggcatagg attggaagac ggtatgtacg
    11641 tcgcgcttgt tggttggaat atcattgata cgaacaccgt ctagcatgat atctcctgtc
    11701 gtcgcatcca gtaaacctgc aataatgttt aggatagttg atttccccga accagatgca
    11761 cctagaaggg tgtagaattt cccttcttcc aactcaaagt tgatgtcttt gagaaccttg
    11821 gtgttgctgt cttcaaaaac tttagagacg tttttgaatt cgataattgg ttttttcaat
    11881 tggtataaat tccttctttt tcatagatta acagatcggg gctctgtcag gtcgccacta
    11941 cctcttgcag ggagtaaaac cacctgcata catcttcgct accgataggc tttcaccccc
    12001 tttccatgac atagcagcag cttttcaaat acagcttcct acttgctttc acccaagatc
    12061 cggacttctc tttcaagcgt aatacctgag tgttccttga ctttttcgat aaccgattgg
    12121 atcaagtcct cgtagtcttt ggccgttcca tctgcgacat tgatcataaa tcctgcatgc
    12181 ttttctgata cttctacgcc accgatacga tagcctttca agccagcttc tgaaattaac
    12241 tgacctgcaa aatgcccgac tggacgctta aagaccgagc cacaagatgg gtattccaaa
    12301 ggttgcttga gttcacgtag gtgcgtcaag cggtccattt cctgcttgat aacctgatgg
    12361 gttcctgggg ctagggcaaa tttaactgac aagacaactg caccagactc ctgaatagct
    12421 gaatggcggt aaccaaaagc caagtcttta gcagacaggg tttcgatttc tccatccttg
    12481 gtcaagacct tacaagactg caatatgtga gcaatctcgc caccataggc acccgcattc
    12541 ataaagacag caccgccaac gcttcctgga ataccacaag caaactcaaa gccagtcaaa
    12601 ctatgacgga gggcaatgcg agttgtttca atcaagttag ccccagcttc tgcttcaatg
    12661 gtatagccat caacagaaac gttattgagc ttgtcacaca agatgacaaa tccacgaatc
    12721 ccaccatcac gaacgatgat attgcttgca ttgccaagaa ccatccaagg gatattttct
    12781 tggttggcaa atttcacaag gcgagccaac tcaaaacgat ttcgtggaaa gaccaaataa
    12841 tcagcctctc cacctacttt tgtataacta tagctatgca agggttcctt aaaacggata
    12901 tcaattcctt ctaagatttc aagcattttt tctcttacag acatgtcact cttcctttta
    12961 caaaattcat tccattatac catttttaga gacatttgac gaccataaaa ataccttgtt
    13021 tggattttgc ataagaaaaa gaggttcccc cctttttatg attttttaca aaagatttcc
    13081 ttggttccat aggcaaccag aacgatctcc agtgctagaa tcacttcaac caagactgga
    13141 tttgtcaacc agcctacttg gaaaagagat ggtgccagat caaagaaggc atgcaagcca
    13201 taggctgcta ggagataaat ccatttcttc tggcgaacag cttggtaaac ccaaactgtc
    13261 aaaagtaatt ggaaaccaag cgccaagatt cgctcaaaac caagcaaata aatctgccag
    13321 accgaaagtg actgaatggt ttttaacata ttttcagaca gtaattgcat aacctgtgga
    13381 ttctgagttt gaactgccga aagaacgatg taaagattga gtaaactaat aaggcctaga
    13441 aaaatcaact ccaagccacc atgccctagg ccataggcca aggcatctgc cttttccaaa
    13501 cttctctttt tctccaacca tttgaagaaa acaagacgag ccgtttcctc aaaaaatgct
    13561 gccatggcta ggccatagat aatatagata agcggatgat cttgcaagag ggcaatacta
    13621 ccgtcttttt gaggatgcaa aatcaagata tgcaccagtt tttccaaaat ctgcgaagag
    13681 acaaagaaag cgactgcacc caagcccaag acagctagat taatctggta tttctttttg
    13741 gcaaaccaaa tgcttcctat cagaaaagct agcaacagca acatggtaat aatgatatga
    13801 atggtcattt tcttctccta ttctgccttt tcaatatctt ttttcatctc gtcaacattg
    13861 aacttagcaa acaagtattg acggtcttgg actgggaaac gttggtccaa ctggtcaact
    13921 gctcccacct cgataatgcc ttccttgatg acaaaatcca tgacatgtcc accgaaggtc
    13981 aaatcatctg atatgaagtg cagatggtag cctgccacac tgaccccatg gaaaatctca
    14041 ggcgtccaga aaccaacaat agtccccaca acattgtcac gactatattc cggttgatgg
    14101 gttgcgacat cagcaaactt ggtatcgggt gttgacttag gaatcatacg cacatgcata
    14161 tgtgaaaatt ctccccgaat cttgatagag cggaaaagat tttccccatc ataataagac
    14221 tcaattcgtt cttccaattc cttgtctgtc atctcaaagc gctggcggaa aatgacctct
    14281 gcctgatgcg gtactactgc tgcataagga ataagggcat ctggtgacac ttccacaatt
    14341 tctggctgtt ctcctgttcc ttttgcctga taagccttgc catctaagac aatcaattcc
    14401 ccatcaatag aatccaaggt tcccaaacca aggtcaccat gctctagcaa ttctcccact
    14461 gtcatggtac caccataaag gccagccatc aaggctccaa gagtattgta ttgaaataat
    14521 ttcactggtt cctgcacgtt cttatccatt cactttcttg tctgttattc tataaatctt
    14581 actcctaagt ataccacatt tgcccctaga tgtgaacgag agaaacgctc tagacattgc
    14641 caagaaggaa aaaaaagggt acaatgtaac aaaatcaagg gaggtctgga atgaagagac
    14701 aaagcaagta caaagaggtc gtttcctatc tgaaaaatgg tatcgagtct ggacgatttc
    14761 cgacgggtag tcgcctgcct tctatccgtc aactgagcct tgactttcac tgcagcaagg
    14821 acaccattca acgagccctg ctggaattac ggcacgaaca atacctctat gccaagcctc
    14881 agagtggcta ctatgtatta gaacaagggc aacatcaaga cctagaaatc gaggttaccg
    14941 acgaacatgc cagtgcctat gacgatttcc gactctgtgt caatgaaacc ttgattggcc
    15001 gagaaaacta cctcttcaac tactatgaca atcaagaagg attagaagac ctaagacagt
    15061 ccattcacaa actcctcttt gagcaagctc tctactgcaa ggctaaccaa ctagtactga
    15121 cttctggaac ccaacaagcc ttgtttatcc tctctcaaat atcctttcct agacaagcca
    15181 aggaaatctt ggtggaacag ccaacctacc atcggatgaa tcgcctcttg attgcacagg
    15241 ggctggacta tcaaacgatt gaacgaggca ttgatgggat tgacttggag gagctggaag
    15301 gccacttcaa aacaggaaaa attaaatttt tctacaccat tccccgattt cactatcctc
    15361 tgggacattc ctattctgag caagacaaac gatctattct taacttagct gccaagtatg
    15421 atgtctatat cgtagaggac gattatctgg gtgatttgga ctccaagaag ggccaaacct
    15481 tccactatct tgatacagag gagcgtgtca tttatatcaa gtccttctcg accagccttt
    15541 ttcctgccct tcgtattaca gcactcattc ttccaaatgc tatcaaagaa gcatttatgg
    15601 cctacaaaaa tatcctagac tacgacagca acctcattat gcaaaaggcc ttgtcactct
    15661 atattgacag tcaattgttt gaaaaaaatc gtttggctcg cttgaccaat catgaatctt
    15721 accaaaaaca aatcgaggaa aggataacta aaacaccttg tccccttcct cattatcccc
    15781 tacacgatgg tttattgcta gacctgagac agtatcctaa aatcgccagt ctcaaacaca
    15841 gtcaactggg cttggacttc tttgaagagg cctatttaag cacctgtcct tatcaatttg
    15901 ccaaggtgtc cctagacaat ctggaaaatg ttttaaacta tttaaaagca gaattggaat
    15961 gacatccaac tctgcttttt cttattcttc aacttcagtt agacttgctg ctttttcaag
    16021 atagctttgg taagttcctt catccttaag tttttggatg accttgtcta ccacttcttt
    16081 caaatcatca ctattttttc taagtgcaac ggcattggcg tcgccgtcct tcatcttcaa
    16141 gctgacagtt gcgacagcta agccagcgtt tttagcagca taactaagtg caacaggctc
    16201 atccatatga acagcatcta tttttccagc ctgcaattca ttgactgctt cacccatatt
    16261 agttagggaa gttaattgag cttttggcaa ttgttccttg accattgatt ctggaacagt
    16321 ccctttttgg gctgcaatat tagcactttc taggctagtt aaatccttgt atttttccac
    16381 atcagcctta cgaaccaaga aactaatctt gttttcatag tatgggattg aaaaatcaaa
    16441 gacttctttt ctctcgtcag tagcactaat tcctgcaact gctaggtcag ccttaccagt
    16501 ttgaagactg gtcaaaacat tgtcaaaact catgcttaag atttccaact taaccccaag
    16561 ttcatcagcg atagcttgag ccatgtcgat gtctgcaccg actacctggt tctttccatc
    16621 aaccaatgat tgaaattcaa agggtgcata gtcaggactg gttgccacaa ctaatttccc
    16681 cttttgctta atggcctcaa cagctgactg agaaccatta gaacctgact ggcaagctac
    16741 taagaagaaa cttgcaagaa aattacataa aacaaatatc catttttttg atttcataaa
    16801 taaacaacct ttctgttaat tttgtataat tataacaata tccaagttac ttgtcaagtg
    16861 ttttttaaat ttttatctca aaaatatttt ttcgttcaaa aaaaggagcc atcagttgat
    16921 ttcaagctcc tttttataca gaattaaact attttatagt tcgacaatct tacctgtttc
    16981 aaagtagaca acccattcac agatattttt agcatagtca ccgatacgct ccaagtagga
    17041 aataacttgg aaataatcac gacccgtaac aatggcttct ggatttttct taatctcttc
    17101 agtcgcaagg tcacggatag tttcaaaata gtggttaatt tgctcatcca tggaggccac
    17161 ccggtatgcg tcgtcaacag aaccattaag ataaagatca agtgctgctt caacaacgct
    17221 tttaacttca cgtcccattt ttttaatttc ttcctctaca gctgggatgc gttcttcccc
    17281 cttcatacgg atggttgcct gggcaatggc tacagcgtga tcccccatac gctccacatc
    17341 tgatacagcc ttaaggacag tcaagactgt acgcaaatct tgagagactg gttgttggag
    17401 tgcgatcatt tcaaatgatt tcttttccag tttcacttcg tattcattta cttctgcatc
    17461 atcttctatg acctcttttg ccaggtcacg gtcatgcgtg acaaaagcac gtaccgtacg
    17521 attgatttgt gagagcactt cttgtcccat agcgtagaac tggttatgta atttctctaa
    17581 atcttcttca aattgagatc gtaacatctt tcatctcctt atccaaattt tcctgtaata
    17641 tagtcttccg tttccttgtg ttggggatca aggaacatct gcttggtatc attaaattca
    17701 atcaaatctc catctaggaa aaatcctgtc ttatcagaga tacgtgaagc ttgctgcatg
    17761 gaacgggtta ccagaagcat ggtgtacttg tcttttagac catacaaggt ttcctcaatt
    17821 ttaccagctg aaatcggatc caaagccgaa gttggctcat ccaagaggat gattttagga
    17881 ctagttgcca agacacgggc cacgcagaca cgctgctgtt gaccacctga caatccaata
    17941 gctgaatcat atagacgatc cttgacctca tcccagatag aggcaccttg caaggctttt
    18001 tctacggctt catccagaac ctgcttatcc ttaattccat tgatacgaag cccgtagaca
    18061 acattctcat agatagtcat agggaaagga ttaggttgtt ggaaaaccat tccgatttcc
    18121 ttacgtaatt caaccgtatc tgtacgcgga ctgtagatgt tgtgaccatt gtacaccacg
    18181 gatccagttg tggtcacctc tggattgaga tctcccatgc ggttgagaga cttgaggagg
    18241 gttgacttcc ctgatccaga tggaccaatc aaggctgtaa tttccttagg ttggaaagat
    18301 agggaaacac tattcaaagc cttcttttta ttataataaa cggacaggtc tgatacctgt
    18361 aaaatcgcat ctgtcatacg gtttcctttc taaccaaagt gaccagatac atagtcattg
    18421 gtggactgta gcttggcatt ttggaaaata gttgcagtct tgtcatactc aatcaaatca
    18481 cccaagtaaa agaagcctgt atagtcactt gcacgagcag cctgctgcat attatgcgtt
    18541 acaatgatga tggtaaagtt tttcttgagc tcaaacatgg tctcttctag ttgcatggtc
    18601 gcaatcggat ccaaggctga ggctggctca tccattaaga ggatatctgg cttaacagag
    18661 atggcacgag cgatacagag acgttgttgc tgaccacctg ataaggtcaa ggctgacttg
    18721 tggagatcgt ctttaacctg atcccagagg gcagcctgac gaagggaggt ttctacgatt
    18781 tcatctagga cttgcttatc cttaactcca gcacgttcat gcgcaaaggt aatattacgg
    18841 taaattgact tagcaaatgg attggggcgt tgaaaaacca ttccaatgtg tttacgcatt
    18901 tcataaacgt tgatttctgg acggttgaca tcaattccac gatagagaat ctgcccagtt
    18961 actttagcaa tatcaatagt atcattcatg cgattgagac tgcgtaagta ggtagatttc
    19021 cccgaccccg acggaccaat caaagctgta attttatttc tttcaaattg catatcaatc
    19081 cccttaatgg attcattttt accatagtaa acatggacat ccttagtaga aagggctact
    19141 ttttcttcag gaaaggtaag gatatgcttc tcatcccagt tatatgttga catggcttct
    19201 cctttaggca gcggttaatt tcttgtgtag atagcttccg aacttacgag ctccaaagtt
    19261 aaaaatcagg ataaagatca ggagcacagc ggcagaacct gctgatacaa tggttccatc
    19321 tggaatagtg ccttcactat tgactttcca gatatggaca gccaaggttt ctgcttgacg
    19381 gaagatagag atggggctag tcacactgag gatattccag ttagaccagt caagagctgg
    19441 cgccgattgc cctgctgtat agatcagagc tgcagcttcg ccaaagatac gaccagatgc
    19501 caagacgaca cccgttacaa tacctggaag cgcttctgga ataacaacat gaaccactgt
    19561 ctcccagcga gaaatcccaa gagccagacc agcctcacgt tgggtatggt gaacgtgttt
    19621 caaactatcc tctacattac gcgtcatctg aggcaaatta aagactgtca aggccaaggc
    19681 acctgaaatg attgaaaatc catactcaaa ctggactaca aagatcaagt aaccaaagag
    19741 acccaccacc actgatggta aagaggacaa gatttcaata caagtccgca caaagttggt
    19801 aacaggacct tttttagcat attcagccaa gtagatccca gctcccatag aaagaggtac
    19861 agaaataatc aaggtaatga ccaataggaa aaaggaattg taaagctgaa tgccaatccc
    19921 accacctgct tgaaaagcag aagaccttcc agtcaagaaa gaccaagaga tatggggcaa
    19981 gccccgaacc aagatataga gaatcaagga agccaagatt gtcacaatga tgctagcaat
    20041 cgtatagagg acagctgttg caagtttatc taatttctta gcgcgcataa tttttctttc
    20101 ctctttcttt cgtaatcaat ttaatcacac tgttaaaaac taaactcatc aagagcagta
    20161 ccaaggccag tgaccagaga acattattat ttacagttcc catgacagtg ttcccaattc
    20221 ccatagttaa tatagaagtt aaagttgcag ctggtgtggt caaggaagtt gggataacag
    20281 ctgagtttcc gacaaccatc tggatagcta aagcctcacc aaaggcacgc gccatcccaa
    20341 agaccactgc agtgaaaata ccagaacggg ccgccttcaa gatcacacgc cagatagtct
    20401 gccagcgagt ggctcccata gcgaaactgg cttcacgata ataacgagga accgcacgca
    20461 agctatccgt tgtcataaag gttacggtcg gcaaaatcat gacaaagagg acggaaatcc
    20521 ctgacaaaat cccaaaacca gtcccaccaa agacactgcg aacaaaggga acgacgactt
    20581 gcaagccaat aaatccgtac actactgaag gaatcccaac caggagttca atagctggtt
    20641 gcaaaatctt cgcccctttt ggtgatactt cggtcataaa aactgctgca ccaatagcaa
    20701 agggggttgc gataagggct gagagaatgg taacgataaa ggaacccaaa atcataggaa
    20761 gggcaccaaa ttctttacta gaaggattcc aagttcctcc caaaagaaag tcaaagatat
    20821 tcacaccatt gacaaagaag gtcgacaagc ctttttgcgc tacgaaaacc aaaatcatgg
    20881 ccacaaggat gactatcaaa gaaagacagg caaaggtcaa accttttcct aatttctcca
    20941 gacgagaatt ctttgatgga agcaacattt tcttagctaa ttcttcttga ttcattattg
    21001 tctcccttcc aacactgtca cagttccggc agcatctttt tcaaccttca tttccttaat
    21061 cggaatatac ttcaatcctt tgacaatccc ttcttgggtc tcatccgaga gaacaaaatt
    21121 gagaaattct gcagccaact cattgggctg ccccaatgta tacatatgct cataagacca
    21181 caagggccaa ttattgctac ttatattttc tggacttaag tcatagccat tcaacttcat
    21241 gcttttgacc gaatcatcta tataggtaag agataaataa gagatagctc ctggactttt
    21301 tgatacgatt gattttaccg ctccatttga atcctgctcc tgactttgca tggcagactg
    21361 accttccata atgacagtat caaaggtagc acgagaacca gagccggctg cccgattgat
    21421 aacagagatg ggtaagtcct taccaccaac ctctttccaa ttggttacct cacctatgaa
    21481 gatttgacga agttgctctg tcgttaggtt atcaacatca acctccttat tgacaatcag
    21541 agccaagcca gctaccgcga ccttgtggtc aacaagagca gaagcatcaa ttccgtcttt
    21601 ttcctcagca aatacatctg agtttcctat atcaactgcc ccagactgaa cctgggacaa
    21661 gcctgtacca gaacctcccc cttggacatt gaccgttttt ccaacatgga tcgtgccaaa
    21721 ttcatctgcc gctacttcaa ccaagggttg caaggcagtt gagccaacag ccgttatgga
    21781 ttctccacga tcaatccagc tagcacagcc tactaaacaa gccgtcagcc aaaaagcgat
    21841 aagagacaga gcaagctttt ttcttttttt cactgttttt ctcctcgaaa ataattatga
    21901 atactgtgaa ttttttaagt agttctttat gagttgacgc atgaattctt accaaatttc
    21961 tgcgcaattg attatttata taatataggc tatattactc tttcctaacc tccttttttc
    22021 atatgtggat aaaatctctt gtctatccct tcccccattg tcacccatta tagtcatttc
    22081 gtgtctcttt ttcccctttt taatgcaagg gaaattactc tccttagatg ataatccaaa
    22141 agctagaaag gtatctcaaa cctctctact ctcccagact agtttacaac taaaaggaaa
    22201 agattcgatt ttatgagaaa tctagtttac aagcggtaag aacgctaata actaaacttc
    22261 ttgtactctt tgaaaatctc ttcaaaccag tgttttgagc tatctatggc tagcttccta
    22321 gtttgctctt tgattttcat tgagtagtaa aactacatgt aatggcaatc aagatatcaa
    22381 gaatcatcct actaaaaaaa tccatacttt cactataaca tagaataaga tatttgacta
    22441 gcattttcat ttgaatctga ggccttttgg aaaataattt ttcaaaacat ttccagtaac
    22501 ctttgcaaag cccaagccat tgcctttaac caaaacttgg taccaaccat ttggcagact
    22561 ttctgccagc tgaacgattt ctccagccgc atacttgaca aactcttctt ggccaatttc
    22621 aaccgactgt tcgacctgac tcggtttcaa ggctaaacca agagcgaaac tgggctcaaa
    22681 gcgtttcttc ttaaaagtac ccagatgcag tccattgcga gcaatcttga gcttccctaa
    22741 atctggcaaa agttctggca agagataaag ctggtctcca aaaatctgca agatacccgg
    22801 tagattgacc ttcaaatggt tttgggcaaa ttcctgccac aaggcaactt gttcacggct
    22861 gaggttactc ttacttgcct taaatttagg agctggattg ttacccttaa actgtagatg
    22921 ggcaacaaac tgaccctctc ccttaaactg atgagggtac atccgagccg tttctggcag
    22981 gtcaatacca gctaccattc cattgatatg ctctactggc aacaagtcaa aatcatactc
    23041 ttccagcaac caattgacaa tctcttcgtt ttcctcgggt gcccaggtac aggtcgaata
    23101 aaccagacga ccaccttcag ctaacatggt cactgcatcc tccagaattt ctctttgcaa
    23161 gctagcacat tgactcggat aatctaagct ccaatagtcc atagcatcag gttgcttacg
    23221 aaacattcct tcaccagagc aaggggcatc aagaacgatt aagtcaaaat agcctttaaa
    23281 gaccttgacc aagcggtcgg cagattcatt ggtcaccacg acatttgtcg ctccaaaacg
    23341 ctccatgttt tcaaccaaaa tcttagcccg tttgcttgaa atttcattgg aaacaagtag
    23401 cccctcccct gctagatagg ctgccagttg agttgatttg ccccccggtg cagcagccaa
    23461 gtccaagacc ttcataccag gactgggttg ggctacttga gccaccattt gagcagcagg
    23521 ttcttgcgaa taaactaaac ctgtagcatg ctcaggcgat ttccctgaaa ccttcccata
    23581 gtggccccaa ggggtttgag taatggcatc agaaaaggaa agttgctctt cttttaaggg
    23641 attgacccga aaggccgaaa ccgcttcctc ctcaaaagag gcaagaaaat ctcttgcctc
    23701 atctcctagt atttctttat atttttcaac aaatccttct ggaaatttca tttaagttct
    23761 tttcctttcg taaatatagg actgaatttc ctcctgcatc tcaagaggca ccatcatgac
    23821 cggctgtctg gtttgaaaat caggagcttc accaaaaagg gtcacaaccc gatagcccag
    23881 actttcccct aaaatactag ctgcggcata atcccatggt tgcagatagg tgagataggt
    23941 caacaaatgc cctgacaaaa tcttggcaaa actaatggcc gcacttccat agacacgaac
    24001 accaagaacc gctcggctca aatcagccag cccccattca ttggtttcca gcataccact
    24061 attccctgca atgagaaaat ctccaagtgg tttagtttta aaaggagcta ggggcctatc
    24121 atttagacaa actggaaatt ccccaccacc gtggtaacaa tcccctttga ccacatcata
    24181 aatcagacca aactgtccct gaccattttc aaaataagcc atcataacag caaaatcttc
    24241 ctgctgggct acaaaattat tggtaccatc aatgggatca atgacccaaa ccttgccctc
    24301 ttgaaccgag gctcgcagac aaccttcttc agcacaaatc ttatcctcag gataacgaga
    24361 caaaatctca ccaaccaaga gttcctgaac ttctttgtcc agtctggtca ccaaatctgt
    24421 tggagaggac ttggtttcaa cacgcaagtc ttcctgcata tggtcaagaa tgtactggcc
    24481 tgctttctta acaagccctt tagcaaattc aaatttactt tccaagagaa atctttcctt
    24541 cccctttttc tttggcagcc tgaactgctc ggtaaagtga atagccacta accgtttcaa
    24601 attctcgtcc caaacgtttc tcttcgctct tgcttggcac gaccgctttg aatcccttat
    24661 aggagtccag taacttttta gcctctactt tgccttcata ggcagcttca acatcattaa
    24721 aaaaagaaag cactgaagca agttcttcag tgctccacga caaatctagt gggtaactat
    24781 actgtttgtt cattaactaa taccagctct cattcttgct tcttttagtt cttgcttacg
    24841 ataactacga gggagaaaag cacgaatctc atcttcatta aaaccgattt gcatacgctt
    24901 ggcatcaata ataattggac gacgcaaaag actaggatac tgctcaatca aatgaagcaa
    24961 ttccgatacc gaaatactct ctacatcaat attcaatttt tgaaaaattt ttgaacgagt
    25021 tgaaatgatg tcatcagtac cattttcggt caaggaaagg atgtgttgca attcttttct
    25081 tgttaaagga ctggtcataa tattgtgttc cacaaaggga accttatgtt tttctaacca
    25141 ggccttagcc ttacgacatg atgtacagct cggtgataga aatagtgtaa tcatgctttt
    25201 ctcttcttat ctatactttg ctacttctat tatacaaaaa aataaggcgc ttgactaggg
    25261 atttttagaa aaaaagccta ttttttcaag aaaaataggc tttttgcgaa cgattgacac
    25321 aattggattt ggttaattca ctcttaacga tggttttaaa cgatatatat ttttatatat
    25381 gtaaattaaa aacatctttc ctttcacttc ctacgacttt tcagatacag atagccaaag
    25441 aagttttcat agagggcaaa aaagaggagg aaggcgtgaa gaaagaaggt ctctggcaaa
    25501 atcataataa caggatcctt ggctggatca aaaagccagg tatcatctcc cacaaagaga
    25561 atttgatgga aaagagtaaa gaattggtca aaaccaatca aaactccccc aagtccaatc
    25621 atcacaggta agactactag agccaggaga ctttttcgat aaagagacaa aaagtccttt
    25681 ttcacaatcc tattgacaaa gacatagaaa cttggcagtg tcactagagc tactagctga
    25741 accaaatgaa agagattctt gaccactgcg aaatggtgca gaccagctgc tgacgaacga
    25801 aaatcaggca tctgtaagac ctgactaaaa ggattggtca gataattcat caagatatga
    25861 aaattgtatt gaacggtttc tggttttaga tagactcgat tcgttaagtt tagccactga
    25921 atctccatag gatagaaaat ccaagccaga taaatggtca gaaggattga gagggagagg
    25981 agaaagagca tagagcccca aaaggtcaat ttagttttca tcaaaatccc actccgcaag
    26041 gctagaaacc acatgtgtcg gtgcgattgg caggccagct acttcttctg ccttagtaaa
    26101 acctgtcgtc accaagagcg ttggaatgcc attgtcaatc ccagcccgga tatcagtcaa
    26161 ataattgtcc ccaaccatga ttaactcttc acgttccaaa cctaagtgct caaccgcctt
    26221 atccataatg atggcatttg gttttccgat ataaaccggc ttcactcgtg tcgctacttc
    26281 aagcagcgta atcagtgagc cagcacctgg caaaagacca cgttccgtcg ggatgttgag
    26341 gtcaggattg gttccgataa aatgggcacc cttttgaata gcaagagttg ctgtggcaaa
    26401 tttttcatag tcgacttgcc aatccagacc aactaccacg taggcagggt tttccttgtc
    26461 ttccacataa ccagccgcct tgatggcttc cttgagtcct gcttctccga cgacatagac
    26521 agtcttttca agccccaaat cattcatata gtcgatggtt gccaaagtcg ctgtgtagac
    26581 agtcgataga ggcgtatcga tattaaaatt ctgagccaac atctccttaa cactctctgg
    26641 agtgcgggtt gtattgttgg ttacaaagag atagggaatg tcccgctttt gcaattcatg
    26701 aacaaaagtc tctccagcag ggattcggtc tttcccctta taaatggttc cgtctaaatc
    26761 aattaaatag cctttatatt tcatctattt ctccctaaac cttttttatt tcttgccaag
    26821 taatgattgc ttgggcattg ataaccccat cacttgtaat ttcatgcttg ctttccagtc
    26881 cagtccgttc aacagccgat gtaatcaccc cacctggtcg aacttccttg acatacttga
    26941 ggttgatttt cttgggaata tagtgggtca aaaaatccgc tcccatgacc tcaaaaatcc
    27001 agtccaagta tttactgtta ttgacatgac cattcatatc caagtcgtaa aaacgaacat
    27061 ggtaatcctt gctgatcggc tcttccaagg attcatactt cggtccacgg ataagttttt
    27121 tatcaaaatc agactggtaa ggagccacaa tctcaggttc aacagcatgg acttttcgac
    27181 tgtcgcggtc catgagaaca aaggtcgcca tcatatggat gagctcctgc cccgcttcat
    27241 tataaatagt aaagcgacgg tagcaaaaaa gtcgattgta gctcaaggct tccgtttcga
    27301 tggtaatttc ttccgcaaaa cgaggcaaac gaaccacctc aatatcatat tctgcgataa
    27361 tccagaccag attatagtct tccaaaatgg ccttatcact aactcccagt tcaatcgatt
    27421 gcatccctga aacttgcagt gacagcaaaa tcacatctgg aagtttgata tgaccgttca
    27481 tatcagccat atcaaaagga attttcattt tcatttgata agttaagccc atgatcctac
    27541 tccaaaataa atcgttctgc tacagtatct cccaaaaaga gacctctctt tgtcatgcga
    27601 acgtggtcac cctcaatctg catgaggcct tgttgaacca aatctctgac aatttctcca
    27661 taaagtccag caaaagactg tccaaatttt tcctcaaatc gcgccatgga aaccccggat
    27721 ttcttgcgga gtcccaagaa catttcttct tccatttgct ccttttgact caggtgatct
    27781 tctgtaatac aagcattgcc ttcctcaacc gcactgagat aatgacgaat gggaccatga
    27841 tttttatagc gtactccatt gacataacca gatgcccctg caccaatacc atagtattca
    27901 gcattgtccc agtacatgag attatgacga ctttcaaagc cgggtttgga gaaattagaa
    27961 atctcataat gctcaaaacc cgctcgctcc agctctgcaa tgatgtactc aaacatctcc
    28021 gcttctagtt cctccttagg cagaggcaat ttcccacgtc gcatccggtt cataaagacc
    28081 gtatggtttt ctaaaatcaa actatacaaa ctcatgtggg gaatatccaa tccaatggct
    28141 ttagccacat tttcctttac ttgctccatg gtctgaccag gcagagcata aatcaaatca
    28201 atggagatgt tgtcaaaacc agccagtttc aggcgatcga tattttcata aatatccttc
    28261 tccaaatgac tgcgcccaat ctttttcaac atcttatcat caaaggtctg gacacctagc
    28321 gaaacacgat tgacagccga atttttcaaa acagctatct tatccgcatc caaatcgcct
    28381 ggattggctt caatggtcaa ctcttccaag acagacaaat ccaagttttt agtcaagcca
    28441 ttcagtaaca cctccagttg cggagccgac agggctgtcg gtgttccacc accgatataa
    28501 agggttgaca acttttcaat atcataagaa cgaaactctt ccagcagatg ctctaaatag
    28561 ctgtcgactg gctgattttt gatgaagacc tttgaaaaat cacaataata acaaatctgg
    28621 gtacaaaatg ggatgtgcac ataggctgac gttggttttt tctgcatagt aattattata
    28681 ccacaaagac tagattccag ataaaaatca ccatccccag atacatagtc cgtccggaga
    28741 tggtgatggt ttattcttct gttatatcaa tcacaatctc ttctgagtca tcaagagctt
    28801 cggctttttc ttgccattgt tccttgagat tatttaattg attttttgat gcttctgtcg
    28861 cttgaaaagc ataggattta gcttgagcaa gtatactgtc cacagtgatt tcacctgact
    28921 caacctgttc ttttgttttc agaacaaaat ctgtagcctg ctccttaact tctgtcagtt
    28981 tttcacagac ttgctccttg gcatactccg gatcttctct caaatcatct agaaaatctt
    29041 gagcctgact gcaaacttgt ttgcccttat cacttgttaa aaacaaggca agagctgcac
    29101 ctgaaacggt tcctaaaagg attgaggata atttacccat aaggattctc cttttttatt
    29161 ttttgaaaaa tttacttgca agacgaagag ctgacagact tgcaccagtc ttgagtgttt
    29221 ttgaaccagc tgatgaagct ttcttgctca agacacgcgc atggtcattg aggtctgaaa
    29281 cagatagaga taaatctgca acagcactga agagtggatc aatcgtagcc accttgacat
    29341 tgatatcatc tgccaagaca ttgaccttag ccaacaactc attggtgtga tgcaaggtca
    29401 catccacatc tgaagtcaag gttttaatcg tcttttctgt ttcatcgatg acacgaccaa
    29461 gcttttgtac agtaatgatc agatagacca aaaagacaat cacagctagg gcaacaagaa
    29521 tatatgcaac ttctaacatt tagttttcct cctctgtaat atagtaaggg gccttctttc
    29581 gattttgata aataacgatc attataccga gaccgataaa gacaactgac agccattggg
    29641 acactcgaag gccgaagaac atgagactat ctgttcgcat accttcgatg accatacgac
    29701 cgaaaccata ccaaatcaag taaaaggccg tgatatgacc tcgtctgaga ctcttccatt
    29761 tccgtctaaa aatcagaatc aaggcaaagc caagcagatt ccatagagac tcataaagga
    29821 aagtcggttg acggtagctc ccctcaatat acatctggtc acggataaag ccaggtagat
    29881 aatccagatt atccactgtt gcaccataag cttcttggtt aaagaaatta ccccaacgcc
    29941 ccaaactttg agcaatcata acgctaggcg ccgcaatatc tagaaaatcc caagtattga
    30001 tgagtttacg gtcagcaaag atatagagca caagagcccc agttatcaaa ccaccgtaaa
    30061 tggccaaacc accattccaa atggcaaaaa tctctcctaa attctgacta tagtaatcaa
    30121 atcggaaaat aacatagtag agacgagctc ctaaaatagc caagggaaag gctactaaga
    30181 taaaatctaa aatatcgtct ggtatgatct tctttctagg tgcttctttc atggtcaaat
    30241 aaaccgcaag aatcaagcct gtcacaatac ataaggcata ccaacgaatg gctaggggtc
    30301 ctagttgaat agcaattgga tcaagcattt tgcacctcat ttcgagctat gagacttgtc
    30361 agtcgttcgt cgaacaaacg ggtagcatca aagcccattt ccttggcacg ataattcatg
    30421 gcagctgcct caatcacaac agagatatta cgacctgttt taactggaat acgaatacga
    30481 ggaatggcta cgccagaaac ttcaagttcc tctgcattgt ttccaagacg atcaaaggtc
    30541 ttatgcgtat cgtaattttc caaatagaca gcaagctgaa cctgtgaaga atccttgaca
    30601 gcactcgcac cgtagagact cataacatcg ataataccaa ccccacgaat ttcaatcaag
    30661 tgtttcaaaa tttcagctgg ttcaccccag agagtaatct catccttggc aaagatatcg
    30721 acacggtcat cggctaccaa acggtgacca cgtttgacaa gctcaagacc tgtctcgctc
    30781 ttaccaattc cactatctcc ctgaatcaag acgcccatcc cataaatatc catcaagaca
    30841 ccgtgcacac tggtacgttc tgccaaacga gaatccagat agctagataa ctctccagat
    30901 aaacgactgg tagctgcacg gctggttaaa atagcaatct tacattctct agcagccttt
    30961 aacatctcct ctggaaccac caaaccacgg gcaacaatga ctgctggtgt ctcaggtaga
    31021 aacatttttt tcaaaacttc ataacggctg ttggaaggca tgctgatcag ataagaccac
    31081 tccttcatcc ccaaaagttg aatccgctct ggtgtatagt agtcaaaata gcctgtcatt
    31141 tcaagaccag gtcgcgtaat atccgctgta ttgatttcct tttcaagcaa ttctggttca
    31201 ccatagacaa tatctagtct aagcttttca atcacttctt ttactaaaac cgacatcatt
    31261 tcctccttca aatgaattct tctctctatt atatcaaatt gtaggaggaa gtttcagatt
    31321 tttgcaggct ttggagtatt tatttaaaag aaaaaggact agatttctct agcctttcat
    31381 ttcaattaga attttttacg tttacgagct acttctgatt tacgtttacg ttttacagaa
    31441 ggtttttcat agaattcacg tttgcgtgtt tcttgaagag taccagcttt agtaaccgca
    31501 cgtttgaaac ggcgaagtgc gtcgtcaaga gattcattct tacgtactac tgttttagac
    31561 atttttttca ctcccttcaa gttcaatatc tatttcattc tacacgtaaa aggaataatt
    31621 gtcaagcaaa aatgcagttt aattttgatt ttttcttcca tttatacaag agttgaaagc
    31681 gttcaataga acattgcttt tttatttttc gagtaagcta agcgcttcag catctgcgat
    31741 gatggttaca tcagggtgat tttggaggct actcgcaggt aggttctcag tcactgggcc
    31801 agatactgtt ccggcaatgg cttctgcttt cgactcacca taagcaaaaa gaataataga
    31861 cttggcatcc aaaatgtttt taatccccat tgaaatagct tgggttggga cgtcttcaat
    31921 cttggcaaag aagcgagcat tggcttcgat agtagactgg tcaagttcta ctagatgcgt
    31981 ttgactgtca aatggagtac caggctcatt aaagccgata tgcccattgc gaccgattcc
    32041 caagatttgg aggtcaactg gatggtcagc caaaatttgg ttgtagcgtt caacttcagc
    32101 ttcagcattg tccttaacac cacgaggcaa gaaactttct ttaaatggtt tttggttgaa
    32161 caagttttct tgcatgaagt aacgataaga ctgtggattg tccccatcaa ggcccacata
    32221 ctcatcaagg ttgacactgg ttagatttga aaaatcaagg tcactctcaa caatttcctt
    32281 gtaaaattca agtgggctac ttcctgtcgc aagtcctaga gtttgagctc cattagccaa
    32341 tttttccttc aaaatctcaa aagcaacttt tccaccttga acttggtttt caactttaat
    32401 aactttcatc ttttcatcct ccatttctgt ctatatttat tatatggtat agaccaattt
    32461 ttgtcaagtg aaaacgattt aatttctaaa aaaagagcgt ccaaacttga aacgtgttat
    32521 aatggatagg attagaactt agaaagaatg aacagatata tgaatacagc tgattttgat
    32581 ttccacttgc cagaggaatt gattgcccaa acgccccttg aaaaacgtga tgcctccaaa
    32641 ctcctcatcg tcaaccgtga gacaggagaa atgcaagata aacacttcca ctctattatt
    32701 gatatgctgg aacctggtga tgcccttgtc atgaacgaca cccgagttct ccctgcccgc
    32761 ctctatggtc aaaaagtgga gacaggaggt catgtggaac ttctcctcct taagaacact
    32821 agtggagacg agtgggaagt tctggctaaa cctgccaaac gcctcaaggt cggtactcgt
    32881 atcagctttg gtgatggccg cctcagcgct gtcgttacag aagaattgac ccacggggga
    32941 cgcattgtcc gctttgaata ccaaggaatt ttcctagaag tcttggaaag tctgggagaa
    33001 atgcctctgc caccttatat ccacgaaaaa ttagatgacc gtgaacgtta tcaaaccgtc
    33061 tacgccaagg aaagcggctc tgctgcagca ccgactgctg gtcttcactt caccaaagaa
    33121 ctgctggcag aaatccaagc taagggtgtt catctagtct atctgactct ccatgtcgga
    33181 ctcggaacct ttagacctgt ttctgtggat aatctggacg aacacgaaat gcactcagag
    33241 ttctatcaac tttctgagga agctgctgcc acccttcgct ctgtcaaaaa aaatggtggt
    33301 cgtgtcatcg ctgtcggaac cacttctatc cgcaccttgg aaactattgg ttccaagttt
    33361 gatgggcaaa tccaagcaga ttctggttgg accaatatct ttatcaaacc tgggtatgag
    33421 tggaaggtcg tggatgcctt ctcaaccaac ttccacctgc caaaatcaac tctggtcatg
    33481 ttggtttctg cctttgcagg ccgtgaatta gtcttagatg cctaccacca ttccatccaa
    33541 gaacactacc gcttcttcag ttttggtgac gccatgttta tttattgaga aagaatttct
    33601 ctaaatcttc taataccaat aaatcgctaa gatattattt caaagaacat ctacaattga
    33661 aactctagct agctgtagaa gaggcctagt acattgaaat taaaatgctt ccccctagct
    33721 tcgaaaatat tgccatagat tgcgttgact ctccaaattg attcatctat attttatttc
    33781 agcttcctat attttcttcg ctgtttgtaa atcaaaatgc aagacacatg agtagcacca
    33841 tatttgttac tcttatctgt cctctcaaga gactattatg agttatttca gaatcattca
    33901 ctactttgac cctgactctc cttagtctca aaatcaaaga cttatactct tcaaaaatct
    33961 cttcaaaccg cgtcaacgtc accttggatt atatatgtga ctgacttcgt cagttctatc
    34021 tacaacctca aagcagtact ttgagcaacc tgcgactagt tttctagttt gctctttgat
    34081 tttcattgag tattaaacaa aaagtgaaca aatctgaatt ctaatgtaca gaagactagg
    34141 cttgttcact tttttatagt cgctataaga tgaccttatc tatagctttt ta
//
LOCUS       DAVHIT010000014        33655 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVHIT010000014 DAVHIT010000000
VERSION     DAVHIT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.014"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(223..585)
                     /locus_tag="VVN16_000534"
     CDS             complement(223..585)
                     /locus_tag="VVN16_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU5571977.1"
                     /translation="MLEVGLNSLINLFTFTFDILYPIVKVGHADDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILCPYLRSIVSTVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            599..745
                     /locus_tag="VVN16_000535"
     CDS             599..745
                     /locus_tag="VVN16_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571978.1"
                     /translation="MKTKEQTRKLAVGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FEEY"
     gene            complement(1337..2026)
                     /gene="rplA"
                     /locus_tag="VVN16_000536"
     CDS             complement(1337..2026)
                     /gene="rplA"
                     /locus_tag="VVN16_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU5571979.1"
                     /translation="MAKKSKQLCAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2234..2659)
                     /gene="rplK"
                     /locus_tag="VVN16_000537"
     CDS             complement(2234..2659)
                     /gene="rplK"
                     /locus_tag="VVN16_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5571980.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKVAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2791..2928
                     /locus_tag="VVN16_000538"
     CDS             2791..2928
                     /locus_tag="VVN16_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5571981.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEIADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2968..3684)
                     /locus_tag="VVN16_000539"
     CDS             complement(2968..3684)
                     /locus_tag="VVN16_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU5571982.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3702..4163)
                     /locus_tag="VVN16_000540"
     CDS             complement(3702..4163)
                     /locus_tag="VVN16_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU5571983.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDHQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(4165..5202)
                     /locus_tag="VVN16_000541"
     CDS             complement(4165..5202)
                     /locus_tag="VVN16_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU5571984.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRVYKEEGIDLPMTTHFAFSVFEE
                     VGHGANSNIPEEVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSEAVS"
     gene            complement(5300..6625)
                     /gene="brnQ"
                     /locus_tag="VVN16_000542"
     CDS             complement(5300..6625)
                     /gene="brnQ"
                     /locus_tag="VVN16_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU5571985.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIANVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6796..6888)
                     /locus_tag="VVN16_000543"
     CDS             complement(6796..6888)
                     /locus_tag="VVN16_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5571986.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6931..7083)
                     /locus_tag="VVN16_000544"
     CDS             complement(6931..7083)
                     /locus_tag="VVN16_000544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5571987.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(7243..7821)
                     /locus_tag="VVN16_000545"
     CDS             complement(7243..7821)
                     /locus_tag="VVN16_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU5571988.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7875..9275)
                     /gene="pepV"
                     /locus_tag="VVN16_000546"
     CDS             complement(7875..9275)
                     /gene="pepV"
                     /locus_tag="VVN16_000546"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU5571989.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9288..9893)
                     /locus_tag="VVN16_000547"
     CDS             complement(9288..9893)
                     /locus_tag="VVN16_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5571990.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            10064..10864
                     /locus_tag="VVN16_000548"
     CDS             10064..10864
                     /locus_tag="VVN16_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5571991.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKEFYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11046..11879)
                     /locus_tag="VVN16_000549"
     CDS             complement(11046..11879)
                     /locus_tag="VVN16_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5571992.1"
                     /translation="MNHKIAILSDIHGNVTALEAVIADAKNQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNILFRKV
                     AYDYEAELEFAKSKGLPFIEMYEELRREDNYQRHNLELLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(11869..13713)
                     /gene="uvrC"
                     /locus_tag="VVN16_000550"
     CDS             complement(11869..13713)
                     /gene="uvrC"
                     /locus_tag="VVN16_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5571993.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNKP"
     gene            complement(13753..14454)
                     /locus_tag="VVN16_000551"
     CDS             complement(13753..14454)
                     /locus_tag="VVN16_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU5571994.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQVSARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14533..15765)
                     /gene="murN"
                     /locus_tag="VVN16_000552"
     CDS             complement(14533..15765)
                     /gene="murN"
                     /locus_tag="VVN16_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU5571995.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15770..16990)
                     /gene="murM"
                     /locus_tag="VVN16_000553"
     CDS             complement(15770..16990)
                     /gene="murM"
                     /locus_tag="VVN16_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU5571996.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17102..17881)
                     /locus_tag="VVN16_000554"
     CDS             complement(17102..17881)
                     /locus_tag="VVN16_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU5571997.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQDFSPESQNLG
                     TPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17950..19611)
                     /locus_tag="VVN16_000555"
     CDS             complement(17950..19611)
                     /locus_tag="VVN16_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5571998.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19786..22008)
                     /gene="recJ"
                     /locus_tag="VVN16_000556"
     CDS             complement(19786..22008)
                     /gene="recJ"
                     /locus_tag="VVN16_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU5571999.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIGANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22285..23043
                     /locus_tag="VVN16_000557"
     CDS             22285..23043
                     /locus_tag="VVN16_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5572000.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYTHKTVLENVT
                     LAPVKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            23054..23848
                     /locus_tag="VVN16_000558"
     CDS             23054..23848
                     /locus_tag="VVN16_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5572001.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSD
                     YGIVTKKSNTEFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23861..24538
                     /locus_tag="VVN16_000559"
     CDS             23861..24538
                     /locus_tag="VVN16_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5572002.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPGFLGAALLYFALCFPVAQFGRKMEQV
                     NKKAYSL"
     gene            24548..25207
                     /locus_tag="VVN16_000560"
     CDS             24548..25207
                     /locus_tag="VVN16_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5572003.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISNLSHMLAKRWQQAA
                     E"
     gene            25351..26541
                     /locus_tag="VVN16_000561"
     CDS             25351..26541
                     /locus_tag="VVN16_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU5572004.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26862..27743)
                     /locus_tag="VVN16_000562"
     CDS             complement(26862..27743)
                     /locus_tag="VVN16_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU5572005.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27884..29212)
                     /gene="vncS"
                     /locus_tag="VVN16_000563"
     CDS             complement(27884..29212)
                     /gene="vncS"
                     /locus_tag="VVN16_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU5572006.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVVQD"
     gene            complement(29209..29865)
                     /gene="vncR"
                     /locus_tag="VVN16_000564"
     CDS             complement(29209..29865)
                     /gene="vncR"
                     /locus_tag="VVN16_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU5572007.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCIFTVRNVGYKLERK
                     "
     gene            complement(29914..29997)
                     /gene="pep27"
                     /locus_tag="VVN16_000565"
     CDS             complement(29914..29997)
                     /gene="pep27"
                     /locus_tag="VVN16_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU5572008.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(30176..31555)
                     /gene="vex3"
                     /locus_tag="VVN16_000566"
     CDS             complement(30176..31555)
                     /gene="vex3"
                     /locus_tag="VVN16_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU5572009.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31607..32254)
                     /gene="vex2"
                     /locus_tag="VVN16_000567"
     CDS             complement(31607..32254)
                     /gene="vex2"
                     /locus_tag="VVN16_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU5572010.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32267..33544)
                     /locus_tag="VVN16_000568"
     CDS             complement(32267..33544)
                     /locus_tag="VVN16_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572011.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYDSSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9561 a         7687 c         6260 g        10147 t
ORIGIN      
        1 taaactaacc tcctcattat tagcaaggct tctactgatt ataacctaat tcttagacta
       61 cttcaagact aactgtccaa taataatcta cagataaatc atccactctt aatcaaattt
      121 tattcaatcc atctactcat tcccaaaagg ttgctgtata atgcaagaca tttttcttac
      181 tgattttact tttccttacc ttcaatttag tgagctaata aattattctt cttcaaaagg
      241 ttgagattta tctagtaact ctaaaacatt aatggtaggt agcaacattg cttcactgct
      301 atcaattgcc gaaactgttg aaacaataga acgtaaataa ggacaaagaa tagcagtacc
      361 atttacgtat agaatatcgg tgatattttc atctatattt atttcaaaat atcctattac
      421 agttacagat atttttttca tggcatcttt gtcaactaaa gaaacactaa gttttacggc
      481 tccatgacta taatcatctg catgaccaac cttaactatg ggatataata tatcgaaggt
      541 aaatgtgaac aaattgatta gggaatttaa accaacttct aacaatgtgt tatactcaat
      601 gaaaaccaaa gagcaaacta ggaaactagc cgtaggttgc tcaaaacact gttttgaggt
      661 tgtggataga actgacgaag tcagtaacca tacgcacggc aaggcgacgt tgacgtggtt
      721 tgaagagatt ttcgaagagt attagaaagc tcgatggact attccagatt cattccacta
      781 gatttcgaaa tcgagtaggg gataatctct agcccctctc acaccaccgt acgtgccgtt
      841 cggcatacgg cggttcaact aacttttaac gcatgtcgtt caaggtaata atccaaacac
      901 gaaaccagtc cacgtttttc aaggactggt tttgatatag cacgtttaag taccgacttc
      961 tgagctacta tagtagattg aaactagaat agtacatctc ttcttctaaa acattgttag
     1021 aaatcgattt ggctgccctg atcgattcgt cctactctta tttcatttta ctataattga
     1081 tagtggtcgc cccagccaga taccttatcc gctatatagg ccttattatc acgttcctgt
     1141 tcgtagagcc acgatttcgc tatcccttcc tctgacgatt cttatttcct gcgtcagact
     1201 tgggaatcgc tattgggttc accggtagca ggtgcccacc gaggacttac acctcagata
     1261 tacgacatgc ccgtcgtaca tcaaaaaaag ttgagacatg gcgtcttcaa cttttttaca
     1321 ttactttaac tactgattaa agtgagttta cgtcaacttt gataccaaca ccttgagtag
     1381 ttgtgatagt caagtttgtt acgtaagttc ctttagctgt agctggtttt gctttttgga
     1441 ttgtttcgtt gaaagcttta aagttttcaa ccaatttttc agcttcaaat gatactttac
     1501 cgatgattgc ttgaacgtta cctgcacggt cagcacggta agtgatttta ccacctttag
     1561 actcttcaac tgctttagca acatccattg ttacagtacc agttttaggg tttggcatca
     1621 agttacgtgg tccaaggaca cgtccaagac gtccaacaag agccatcata tcaggtgtag
     1681 cgataactac gtcgaagtcc aaccaaccgt cgttgatttt agcaacaagg tcatcttcac
     1741 caacaaagtc tgcaccagca gcttttgctt cttcagcttt tgcaccacgt gcgaaaacaa
     1801 gaacacgtga agttttacca gtaccgtttg gcaataccat tgctccacgg atttgttggt
     1861 cagctttttt aacgtcgatg ttcaagttgt aagcaacttc tacagttgcg tcaaattttg
     1921 caaagttagt ttcttttgca agtgctacag cttcttctac actgtatgct tttgtgctgt
     1981 cgattttctc aagagcagca caaagttgtt tgcttttttt agccattttc tattctcctt
     2041 gtaagtggtt caatcgattt tcatctccca cgtcacttct cgaaatgatg aagtcttgcg
     2101 ggttttcagt ctactatttt tctcttccta cggccttaag ctcccttgct gtacccaagt
     2161 acaagcctca gtcacttgcc tagtatgaaa agcaactaat agactgctgt gagattttct
     2221 actgtgttat tgattagtca acaacagtga atcccataga acgagcagta ccttcgatca
     2281 tacgcattgc agactctacg tttgctgcgt tcaaatctgg catcttagtt tctgcaattt
     2341 cttgtacttg tgcacgagta actgtagcaa ctttagtttt attaggtgta cctgatcctt
     2401 tttcaacacc tgcaactttt ttcaaaagaa cagcagctgg tggtgttttc gtaacgaaag
     2461 taaatgattt atcttcgtaa actgagataa caactggaat gatcatacca gcttggtcag
     2521 ctgtacgagc gttgaactct tttgtgaatc ccatgatgtt gataccagct tgaccaagag
     2581 caggtccaac cggtggagct ggtgtagctt taccagcagg gatttgcaat tttacaagtt
     2641 tttcgacttt tttagccatt tttaaatcct cctttgtggt tttggcggta attagagatt
     2701 tttacctccc acaagtatgt ttttcacata cccctccatt atacactatt tatctggaat
     2761 tgcaagagaa aagtttattt tttatactca atgaaaatca aagagcaaac taggaagcta
     2821 gccgcaggct gctcaaagta cagctttgag attgcagata aaactgacga agtcagctca
     2881 aaacactgtt ttgaggttgt gaatagaacc gacgcagtca gtaggtaagg cgacgctgac
     2941 gtggtttgaa gagattttcg aagagtatta atcgacgtag tctccgcctt caaagccata
     3001 gtactcttcc aaactgaggc cacttgcagc aatttctttg gcttcttttc caacgtagcg
     3061 gagatgccat tcttctgcca tgtagccagt ctccttttcc ttgcctttaa gatagcgaac
     3121 aacaaagcca tagtcagccg catggtccaa gagccactgg gctgcctttt cttcagtaac
     3181 taaatcccca tctgtcccaa tcaaatcaaa agcaagaccc gtctggtgtt cactgtaacc
     3241 tgggcgtgct gagtagcgat ctgctgcttc cttaccatct tgattgacat agtcttggta
     3301 gagcttagcc tgagtttcat aactcctaaa cccactgtat tggtcactga ttggatagcc
     3361 ctcagcctgc atagctgcga ttagtttgag caactctgcc ttggctgttg gattttctcc
     3421 tggattgtaa tcttttgaca aaggataacg tttattagct aggataatct catcgtattt
     3481 cccttggata ctataatagc tccctttatt gctaacttct gcctttttct gactagaagc
     3541 ttgagatttg ctacctacag ttccttccgg ttgactgctt tgctccgttt tttgagtgtt
     3601 ttcttcaact ttggtttttt cttgcgaaca agctgcaagg gtcaatgcta gtaaaccaga
     3661 taaaatgaca tatctttttt tcatttcttt cttcctctct cttaggccaa atacttctcc
     3721 aattcatgag ataagatttt aatcatctct tccagctctg gcgattgtac ttggcaatct
     3781 tctgccgcca tttcttccca gggcacccac cagactgggc cacggccatt caaaccgtga
     3841 ctcttcatat cacctgctct tcggggaaaa agatgccaat gagcgtgggc atcgccattt
     3901 cctaggagtt caatattcat cttttcagcc tcaaatgctt tggcgactgc ttcttggact
     3961 aaactcattt cctctaaaaa gcgaagtttt acagaagttt ccatatgatg gagttctgtg
     4021 acatgatctt ttgctaaaaa taaggtatag cccttaaagt attggtggtc accaataaca
     4081 acatagcctg tttctagttc ttttacaaag tagggatttt gcccagcttt aatgagttcg
     4141 attctctgac agattaagca cattctatga taccgcctcg ctcttaagat aagcatcaac
     4201 catacgctca gtcgctacta ctgaatcgat gtgggtgcgc tcataagagt gactggactc
     4261 aataccagct ccaagaagtg catgcttgac ctctgcacct gctgacatcg ctgctgaagc
     4321 gtccgatccg taaaatggat agatgtcgag cttaaatgga atatcttgct ctttcgccaa
     4381 agctactaag tgttgacgga agtcatagtg ataaggaccg gaagcatcct ttacacagat
     4441 agatactgtg tactcatccg tctgttggtc atcgcccata gctcccatat ccacagccag
     4501 atattctact acctcttctg gaatattgga gttagcacca tggcccacct cttcaaagac
     4561 tgaaaaagca aagtgtgtcg tcatcggcaa gtcaattcct tcttctttat aaactcgaag
     4621 gagatttagc aaaatcgctg cgctgacctt atcatccaaa tgacgagact tgataaaacc
     4681 tgtatcagtc acaacagttc gtggatcgaa gctgataaaa tcaccgacct caatcccaag
     4741 agcacgagtt tctttttcat tgcttacttt tacatccaaa cgcacttcca tattgtcctg
     4801 cgtgcgctct gcagttcctg catccttgta gacatggcaa gaagtttggt ggatgaggat
     4861 ggttcctgat accttttgac ctgtgctagc cacatgaacg gtacagtttt caccttcaat
     4921 catgttccaa ggaaaaccac cgatacggtc caatttaaga cgaccatctg gtttgacagc
     4981 acgaacaata gcacccagcg tatccacatg agcagtcaca tagcgatgtt gctcatcatt
     5041 ttgacctttg atggtcacat tgacaccacc cttagctgtg cgaactggct ggtaaccaaa
     5101 accttctaga gtcttgacta aataattcga aatttcacga gtaaagcccg ttggcgatgc
     5161 gatggctgtc agttctttga tatattctac tgtttgattc atttcttcac ctctttcgct
     5221 acctattata acacatttat catcggataa aaaaagaaaa tcactcctgt agacttggct
     5281 acaaaagtga tttaagtgat tattccattt caaaaacatc gctttcttgc ttgtttggta
     5341 gaaccaatga gagcaagatt ccaacaatgg ctggcaccaa ccatgggaga gatgccttgg
     5401 caaaaggaag aacgctaaca agatttgcaa gaaactcaac cttaaacgag cttcctagta
     5461 cgtttgcaat ggcaataact gtaaccacag caattgtcaa ctgcatacct ggttttgaaa
     5521 gggcaacaaa tttgttgaca atgacaatca taacgatagc aatcgtgatt gggtacaaga
     5581 taaccagtac tggaattgag tacttgataa tcgcatcaag acccaaattg gcaatagcaa
     5641 atccaatcaa ggtaaaggct gtcgcataaa ccttgtagct gatttgtggg aagcgctcat
     5701 taaagaactc agctgttgac acaatcaaac caacagtcgt tgtgaagcag gttacggtaa
     5761 ccatagctgc aaggaagagt tgagctgttg agccaaagat ttcttgagtg gcttgtgaca
     5821 agatgtaaac acctggtgtt ccacccttca tcgcttcagc tggtactggg aaatgatttc
     5881 caagaaaacc taaaccgatg taaagagcgc tgaaggcaag ggcaacaacg ataccaacaa
     5941 cccaaatagt tgaaatgtat tctttcttac ttgaaaatcc aagttgtttc aaggtttgaa
     6001 ctgcgattac gctaaaggcc actgaggcaa gggcgtccaa ggtattgtaa ccttctagga
     6061 aacctgtacc aaaggcagaa gcttgataag cagctgaagc agcttgagga cttgttccac
     6121 catatttgat agctcccaga acgaccaaga taacaatcaa aattgcaaag actggcgtta
     6181 aaatacgtcc aatgcggtct aagatttttg atggattaag cgaaatcaaa taggctgccg
     6241 caaaatacag aaccgtaaat acaatcaagc caagtccttt atttgcatcc gacaaaaggg
     6301 ggctaatccc tacttcgtaa gctgttgtag cagtacgtgg ggtagcaaag aatggaccga
     6361 ttgacaagta aagaactgag aggtaaagag tcgcaaacca aggcgctatc ttcgttgaaa
     6421 tctcgtagat atatccttta ggatttagcg ttccaataat aagggtcaag acggcgatac
     6481 caacgcctga aaagacaaaa cctgcgatgg caggaagaaa atgttctcca gatagagcac
     6541 ctagagaagg cggaaaaatc aagttccccg caccaaaaaa tattccaaac aggagcaaac
     6601 ctgttagggt acctttttta gccataaaaa tctccttcat atttataaaa tactgaccta
     6661 gtataccacg attaggagtc tgaaaaaagc atcacgaggc aaatattaaa tgttttcaat
     6721 tttcttaaaa ataagaatta tctaaaaata aaactctcct acccagtcca caccaagtag
     6781 gagaaatgca aaattttaaa gttcttcaaa ggctaacatg ccaccctgga cattgataac
     6841 attataacct tgttctaaca ggaattggca agcacgcgcc gatctcattc cagatttgca
     6901 aataatataa tgcaagcgat ctttgtccaa ttaatcataa ctatcagcca attgactaag
     6961 cggtaggttg tgggcacctt ctaaatgaag agctgcaaac tcatccactt ctctcacatc
     7021 cactagagaa agctggtcgt tttggtaaag ctggtaaaat gcgtcaaagg ctatttcttt
     7081 cattcttttt cttctaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac
     7141 cgtgcgtatg ttaccgactt cgtcagtttt atctgcaacc tcaaagctgt actttgagca
     7201 gcctacgact agcttcctag tttgctcttt gattttcatt gactataaaa tggttttaat
     7261 tctttttttc aaatctggca ctacttctgc ctcaaaccaa ggatttttgg ccatccagat
     7321 ttgatttcgt ggtgatgggt gaactagcgg aaaataggct ggcagatagt ctttatagtg
     7381 tttcaccctc tccgttacct tcccactgat tttctcctgt aaatagtagg cttgggcata
     7441 ttgcccaatc aagagggtta actgaatatc aggcaattcc tgtaagagct gcggatgcca
     7501 tttttctgca aaacctgtac gaggcggaag atcacccgac ttgccatgtc ctggaaagta
     7561 gaaatccata ggcaaaacag caaaataacc tgaattgtaa aaggtatctt catccacacc
     7621 tagccagtcc cgcaagcggt caccactttt atctttccag taaaggcctg cttcttgagt
     7681 tttaagtccc ggagcctgac cgatgatatt gatgcgagca gtttttggcg ctgcaaagag
     7741 aggctcaatg ccacgctctg tatagctggc attctgcgaa tccgccatga tagcctgttt
     7801 gattctttcg atttgagaca tctactttcc ctccaaaaag aagtccaagc ataagatctc
     7861 agacttctat cgttttattt gatcaattcg taaatagctt cggcataaat tgctgctgct
     7921 cggaagagat cgtccaaggc aataaattca ttggcttggt gcatggtatc aattgagtct
     7981 gggaacatag caccataggc aactccgcgc tctaacaagc gaccaaaggt tccaccaccg
     8041 atgacttgtt catgaccttt aaggcctgtt tgtttttcat agacattcaa caaggtttgc
     8101 acaagtggat cttccattgg cacatagtga ggggtatgac catgttctga tagactgaca
     8161 gaagcaactg gcaagttttc taggattgac ttgatttgtt ctggacttgt tccttttgga
     8221 tagcggatgt taagggcaat ggtattatca gcacttgttt catcgaagcg gaagacgccg
     8281 gcattcatag aaagagcacc catcttttca tccacatgag caatcttgag attttcaccc
     8341 tcatgatcgt tcaagagaat tttacctgcg atgtcaaggt agtctttggc tggaccagca
     8401 aagccaaact ggctgaggaa gagggctagg taagtcgcac cattgacacc tgaagcaggc
     8461 atagcaccgt gggctgattt accaatgatc gtcaccttgt atttgccagc ttcttcttgg
     8521 agttctcctc taagtttgtg ttctgcaaca aaggcatcta gtttagcttg caagtcagcc
     8581 aaatcacctg aaacgactgc tgttgctgat tctggtacca tattttcacg taaaccacct
     8641 gtaaagctgt gaagacgggc agcacctgta ttttctcctg caaagtggag gtattccgtg
     8701 atatttcctt tttcaccatt gatgattgga aattcagcat ctggtgagaa accgaaatct
     8761 ggtttggcaa gtcctacgtg ctcaaagtag tagtccatgt ctgcccagcc tgattcttcg
     8821 tctgttccaa cgatgaagcg aactttctta gaagttggaa gacccaattc tttgatgatt
     8881 ttcaaaccat agtaacaagc tgttgtagga cccttatcgt ccgaagcccc gcgcgcataa
     8941 aggcgaccat ctttgatagt tggtgtgtaa ggatctgtat cccaaccgct accagctgga
     9001 accacgtcca tatgggcaaa gattccgaga acttcttctc catcaccaaa ctcaaaatgt
     9061 cctgcatagt tatcaacatt tttagttgga taaccatcac ggtctgcgat ttcaaggaat
     9121 ttttccaagg cttttactgg accaggtcca aatggatgtt gggcatcagc cttgctatca
     9181 tcacgttctg agttgatttc caaaaggcta aacaagtcag ccaagaggtc ttctttgcgt
     9241 ttttctactt ctgctgtaaa atcaattgct atcatttttt cttcttccta tcttttttcg
     9301 atgatttcat ctactggcaa gcggtagctt ggttccaatt tgtcgtctgt gtaacccact
     9361 gtgatcaaga gttctgggcg gaaacggtct tcgatttcca aaacttcatt aacttttgat
     9421 ttgtcaaaac caagaataat gttagaacca attccttggt ctgtcaatgc aagaaccaag
     9481 ttcatggcaa ccaaacctgc attgagagct aggtagtcgc tgacttgttg ctcactgtaa
     9541 cgggcaaact cagctggcag atttttcata aaatattgaa gttgctcttc agaaaagtta
     9601 ttagcaccac caacacgggc aatcttacga gcacgtttgg ctaagtccgt atctgtaaac
     9661 aaggcaatgg ttacaggcgc tgatgatacc tgttcaaaat tggaaccata agctaacttt
     9721 gccagttcag catttttctc acgtaccacc acaaatttcc aaggctggct gttgtgggcg
     9781 cttggcgcca aggttgcaat ttcgatagcc gtacgcacat ctttgggatc aacaggctta
     9841 tcagtaaaat gcttagtcgc atgacgtttt ttatttaatt caagaaattt cataatcgat
     9901 ttccttttct aattctactg cttccattct accataattt gaaagagctt gcagggattt
     9961 ttcatgatta agtttttcta tcacagacat aaaagatata tattggttta tcaggaaagt
    10021 ttctgataaa ctagcaagta ctttacattt gaatggagac agtatgaaaa aatttagcct
    10081 attactagct atcctaccat ttttggttgc ctgtgggaat caagctacac ccaaagagac
    10141 tagcgctcaa aagacaatcg tccttgctac agctggcgac gtgccaccat ttgactacga
    10201 agacaagggc aatctgacag gcttcgatat cgaagtttta aaggcagtag atgaaaaact
    10261 cagcgactac gagattcaat tccaaagaac cgcctgggag agcatcttcc caggacttga
    10321 ttctggtcac tatcaggctg cggccaataa cttgagttac acaaaagagc gtgctgaaaa
    10381 atacctttac tcgcttccaa tttccaacaa tcccctcgtc cttgtcagca acaagaaaaa
    10441 tcctttgact tctcttgacc agatcgctgg taaaacaaca caagaggata ccggaacttc
    10501 taacgctcaa ttcatcaata actggaatca gaaacacact gataatcccg ctacaattaa
    10561 tttttctggt gaggatattg gtaaacgaat cctagacctt gctaacggag agtttgattt
    10621 cctagttttt gacaaggtat ccgttcaaaa gattatcaag gaccgtggtt tagacctctc
    10681 agtcgttgat ttaccttctg cagatagccc cagcaattat atcattttct caagcgacca
    10741 aaaagagttt aaagagcaat ttgataaagc gctcaaagaa ttctatcaag acggaaccct
    10801 tgaaaaactc agcaatacct atctaggtgg ttcttacctc ccagatcaat ctcagttaca
    10861 ataacactct tcgaaaatct cttcaaacca cgtcagcttt atctgcaacc tcaaagctgt
    10921 gctttgagca acctacggct agcttcctag tttgctcttt gattttcatt gagtataaga
    10981 tatactaaaa gcgatgggat cagccaatcg cttttttagt ttgcattggt tattttagga
    11041 aactcttaca aaaaatcaaa aaaattcttc acatcctcta catacctatg cttttctatt
    11101 aagcttgcta atagttccag attgtgcctc tgatagttat cttcacgacg tagttcttca
    11161 tacatttcga taaagggaag ccccttggat ttggcaaatt ctaactcagc ctcgtaatca
    11221 taagcgactt tacgaaatag gatatttacc aattccccat cttcaacctc tatcacggca
    11281 tactgggcac ggtgattttt taacgcctcc caattaaaat agggcatgcc aatcgaccct
    11341 ggattgatga tttgttgccc ttgactacca taacgaagca actgcttgtg aacatgacca
    11401 taaactgcca cgtccgtttc cgcatctagc agttggtcaa atttctctgt atcattctca
    11461 actagcaagt caccaccata gtttttgtca ggtaaattat gagagataga aaagcgcaat
    11521 ccgtcaattt ctttcttttc cagcaaaggc aagcttcgta gccagacaat cgttgcagga
    11581 tccattcgct ccatcaaata ctgtgtcata cgcaagagct gaacttcctg tgggtcttct
    11641 aagccatatt gcccatctaa agcctcaagg acacgatcat cccaattgcc tcgaacactt
    11701 gctgtgatag gaaggtcctt tagcagggcg actaagtcat ttgcgcctgg accaggaaga
    11761 aaaatatctc ccagaagcca gtattcactg actccttgat ttttagcatc tgcaatcact
    11821 gcttctagcg ccgtcacatt gccatgaata tctgataaaa ttgcgatttt atggtttatt
    11881 gtggtttcct tccgtttggt aatctactct ttcttctgct acttgaggca aggcttctcc
    11941 ctgcgggttc aactttcttt gcacagcctc tgcaacgact ctaggtaccc caacttcgac
    12001 aatctcatcc acactggctt ccttgatttt ggtcaaagac ttgaaatgct tcataagatt
    12061 ttgcttgcgt ttaggtccca gaccatcaat cccatccaat tgagatgaga aagaattttt
    12121 ggagcgcagt tggcggtgga aggtgatggc aaagcggtgc acctcatctt ggatgcgttg
    12181 gaggaggaaa aattcctgag aattgcgaga caagtccacc acttccagcg gatctccaaa
    12241 gagcaattca tgggtttggt gcttatcatt cttttgcaga cctgcaattg gaatatccaa
    12301 gcccagttct tcttggatga cttgcttagc aatattgact tgaccttgcc ccccatcaat
    12361 cacaatcaaa tctggaggag tcaaagcctc acgctgtact cgaccatagc gtctgcgaat
    12421 gacctctcgc atactggcat agtcgtctgg cccgactacg gtcttgattt tatacttacg
    12481 gtaatccttc ttactaggct tgccgttgac aaagaccacc atggctgaaa caggactagt
    12541 tcccatgatg ttggagttat cgaaggactc gatgcgaact ggggttggga tttgaagcaa
    12601 gcgtcctaga ttttcaatag ctccttgggt cttctcgaca gatttctcta gcaggttgaa
    12661 cttctgctct aggctaacac gggcattttt tatagctaaa ttgaccagct gctttttctc
    12721 tccacgttga ggcttaagaa tcttggaatc caccaaggcc ttgacagcct cttcgtcaat
    12781 atcctgcgga atcagtacct cattgggaac taggtgagat ttttcttgat agaattgtcc
    12841 cacataggtc aagaaatcct catctggatc attgaagtag gggaagagat tgacatcgcg
    12901 ctcgatgagc ttgccctgac gaacaaagaa aacctgcaca cacatccagc ccttatccac
    12961 atagtagcca aagacatcgc gattttggag atccttagcc atgacccgtt gcttggttcg
    13021 aagcgttcca atagcctgaa tcaggtcacg gtattccgcc gcacgttcaa actccatagt
    13081 ctgggctgcc gctgccattt tgccctttag ctcatcaatg attttgttat cctgcccttt
    13141 cagaaaatca gaaacctcct gggccataga cttgaaataa gcctcatcct tcttacagat
    13201 ggtgtgagcc atacactggc cgatatggta ataaaaacag accttagagg gcgggttggt
    13261 acacttacga aaagggaata tccgatccag caaccgcttg atttcattgg ctgcccccac
    13321 atcgggataa ggtccaaaat aaagacctcc gtcctttttg acctgacgag tgataatcaa
    13381 gcgtggatag cgctcattgg tgattttgat gaaaggatag gacttgtcat ccttgagcat
    13441 gatattgtac ttgggcttgt tttccttgat caggttgatt tctaggagaa gtgcctcaat
    13501 attagactcc gtaacaataa attcaaaatc cacaatttca gacaccagag cctctgtctt
    13561 ggtatcatga cttccacgaa aataggaccg tactcgatta cgcagatttt tagcctttcc
    13621 tacatagata atggtgccat ttttatcctt atgaatgtag caaccagggc tggtcggcaa
    13681 gagctctagt tttgatttaa tcaagttgtt catagttcca ttatagcaaa aaaagtaggg
    13741 aaagatagta ccctactcac tagtctcata tatttttcgg aggtttgcaa catcctcttc
    13801 ctggatacca taaaaggaac ctgctggttg catgacagac ttctcatctg tatggtccaa
    13861 gccaatcgca tgacctaact catgttctgc cgtatggaca aggcgttcat aggagtagcc
    13921 atagtatgga ttggacaaat aataatgatt caaccgcacc gttacggaca agaattgccc
    13981 tgttaagaga ttagtttgac tttccgcctc tcctgccaca ggagtgcctc cgtcgttcat
    14041 ctccgtagcc gtaatatccg ccttactaga ctcagtcacg agttcaaagt taaaagcacc
    14101 agtttgattc cagttctgga ttgcttctaa ataagcctct tgaaagcgtg aatccatctg
    14161 cggatccagg taaatacgag cagagacctg tggccacctt ccttcagaat gctggtgact
    14221 atctgtctgg cttaacttag gcagttgccc tgttttctcc cattggtcaa tactttgttg
    14281 accaattttt actgaccgtt ctgcttgctt tagcgctcct tgaaaatccc cgttcagata
    14341 ccagagaagt ccgaaagcaa gcacacataa gagcacaact atccaaacca gacgccaaaa
    14401 caaacgccac acaaaagaaa agaaagcccc tatcaaacga aaaagccagc gcatgtctct
    14461 ccacctttct agcaaataaa aattatttta ctaaaagaag ctaaaagaaa tctaaatact
    14521 gactttttca tcttaacgtc ctactatttt tttgagtaac tggatagctt tgtattttaa
    14581 aggcgatgga tggtaacgga aagtacctgc tttgcgtaca atatagccat taaaattctg
    14641 tttaaaacgc aaaacaccat cacttccatc aaaaatccct tgaatgccta ggaagttgta
    14701 tttaggtatt ccacgtttta tgctttccaa cataacatat ttttgaagca gggcaggggc
    14761 atagaactta ttaaactcag tgtaggaacc actaaagaga taagtcgttt cctgaggcat
    14821 ataaacaaat aaactcccag ctaaaacaat atcttcttct ccatattttt caatcaagtc
    14881 tcgcgcttct gcttttcgaa cttcaaacgt ttcaaattga ctagaatatt ctctcagttg
    14941 attttgtttt ttctcagaat gaggattttt actcaaatca agtcgcaact tgtccaagtt
    15001 ttcttctagt ttactttgtt caccttgcaa tttgctcata tagtccgaaa aattcaagct
    15061 tgctatgaga aactccgctt gttctccaaa agtatcataa aaatgctcat aatattctaa
    15121 acttttatca ctatattctc tacgttcaga ggtttctttt gttatattct taaaaatcga
    15181 tagttcttca cgttttaact ttttcaaccg aatgccaaag gtttcagcct ttttcaccaa
    15241 gggtttaccc tttttgctaa aacttttaag caaactcttt tcagttaatt cagttaaatc
    15301 tttatagtat aaccaatctg gttctccacc tgggtaacct gttgttaagc catcaaattg
    15361 ataacctaaa tcagtcaaac cttgaataat actttttttc tcagcatcta ttggattacc
    15421 ttggctatca aaagtttgat aagtttcata aggttttaca agcaactcta atacaccatt
    15481 ttgcttggca tattctttta actctgcata aaaaactgga agagcatctt gttgggtata
    15541 aatcggcccc gaattgagtt ccatatgcag accacccagc atgggcaagc tataaaccag
    15601 agctgcaact tgaatttctc cttcttgttt caaagcaaga taaacaattc gagcccctct
    15661 tttttctagc aaatccccca tctggacaga ttgcataaag gaacgagaag aaacctgatc
    15721 agaataagtc tgaaactctt ctttcgtgag tgttgttagt gccatatact tactttctat
    15781 gtttttttct taatgtttta cggaaatcaa gagcaagtct taacagagga tagagaggat
    15841 gagtgggcat tgtaaattca cccaagtatt cttcaatcgt tggattaaat ttttccttaa
    15901 aatgataaag tccaccattg agagagtttt caacaccacc taaattttgc cagaccatac
    15961 ctcgctcaaa ggcatagcga gccgtttcat accatgttaa aattggtgca ttgtaacgtt
    16021 taaaatcatc atccatacca gcatatatat tgacagaggt agtaccaaat tccaaactca
    16081 aagtagccgc taaaggaact ctcgcttgac ctacatctat atattcctgc aagaaggtca
    16141 attcctctaa caaacgttct ttttccttct tctgcgcttc tacttttgaa attcgagtcg
    16201 actcagtaaa ggtctcttcc aaggctctat ttttcgctaa ctgttcttct aactcttgcg
    16261 aacgtttaga aacatccaag gtggccaagg tgatataggc cttgtcctta aaattatcta
    16321 acaatttttt ataataggct tcattcctca aatgaatctc ttttcgcttc tcagtttttt
    16381 tcatcaactc cgaaaatgaa tctaatagtt ccagtccacc atattgaatc tcaagccctt
    16441 tgtttcgtgc tgttcgaata gcctgttttg ttgacttgga aagtttatct tcttcaaaat
    16501 tttccttgta tattttcgcc tgaatacgag gttgaatggt gtctcccatt tcctccgttt
    16561 ttcctgacca ccttactccc atttgttgca aactatcaat aatagccaga ttttcaggaa
    16621 attctgtctt ttcctgattg attaaacttt gagataggca aatacttggg tcaaaagtca
    16681 caaaaaccgc tctcttactg cgagcatagg acttaataga ctgaatggca aaattcaaga
    16741 gttctttatc cccataatcc aatataggtc ctcttgggat gtaaaacatt ttatagccta
    16801 gcggaagagt tctaatcaaa atactagctg tcgccagtaa tttttcttcc ctgtaaacac
    16861 caaacttctc atgttgccaa ttagacttaa cttcctccca agcactactt tgtaatacat
    16921 tggctaattc atgttctttg acaaactgat catattctaa tgtgggaatg ccaatttgat
    16981 aacggtacat ttcctactct ctttcctcca gtattttatt actattctac tactttcgtt
    17041 tgaaaagtct agtgaaaaca tattttattt tagttctact tcccctatgc tgaagttcaa
    17101 actaagtcag tctctcttct aatttgaaat caattggtag ggttgttaaa aaaacttcca
    17161 attgtttttc ccagtagtac cactcgtgag ttccagcgct atggctatag gtcacatcaa
    17221 aacctagttt tttgagattt ttcactgcaa gattattggc ttcgtacaag aaatcctgtt
    17281 cgccacacca cgcccaaagt ttggtctttt tatccgattt tttagccaga ctttcaagag
    17341 aatagggact agttgtccag tctttaatct ctccaaaaac acctctccag taggctggcg
    17401 ttcccagatt ttgactctca ggagaaaaat cttgaaagct caatgcacct gaaaagctag
    17461 ctgcatgaga aaaacgattt gtcgcaagag ccagtttgaa acagccgtag cctcccatag
    17521 aaagaccagc gataaaggtc ttttcacgct tgctagtcat attagggaag aagcgtttca
    17581 gaacctgtgg caattcctct gctagagccg tgtagtagtc aaaaccatac tgggtatcgg
    17641 tgtaccaacc attgctggta ttgggcataa caacgatgag attagttcct cgaagcaagc
    17701 gttctacatt ggtccgctta agccaactat tatgattgcc agacatcccg tgcaaaaggt
    17761 acaagacggg aatatcttca cactctggtt cttccactcg attggcatca gggtagagga
    17821 cattcacccc ccactccata tccaatactt gtgagtaata ctcgattttc attactgcca
    17881 tgattttctc cttctatttt tctataagaa aaagccgaaa ctcgactttc tctctgttta
    17941 ttttaacgat tattttgctt ccatgactac tggtaaaatg gctgggcgac gcttggtttg
    18001 gtcaaagaga tacttggtca aattatctcg aaccttccct ttaagatctg cccagtcaaa
    18061 gtcatctcct tgaagatact cttctaccgt ttggttaatc aattctgaac tttcacggag
    18121 aatatcgcga ctcttcttga gataaacaaa tccacgcgtg tgaacacgag ccctagccac
    18181 aattttcttc tcacgacggt tgactgtaat agccacgatg aaaattccat cctctgacaa
    18241 gaccttacgg tcacgaagaa caacatttcc aacatcacca atggcattcc catcaatcaa
    18301 gatatctcct gctgaaaccg atccagctgg aacaaagtct ccattctcgt aagccatggt
    18361 cgtccccttt ttaggaatga agatgcgttc tggcaacatc ccaactgcca tggcagcctt
    18421 agcgtgagca tccaactcac gatactcccc ttggacaggg aagaggtact taggttgcaa
    18481 aagattgatc atcagctgca aatcacgcac atttccgtgc cctgatacat gtaaactttg
    18541 ggtaatcaat ttgacaaccc cacctgcctg ataaatcata ttttccacac gcgcaacaaa
    18601 ggcttcttta gcaatagacg gagccgtagc aatatagacc aggtccccat ccttgatttc
    18661 tacataacga tggcgaccaa tcgacatctt acgaagtcca ttgataggtt cacccatacg
    18721 acctgtctca agaataatca actcatggtc ttcaaatcga gacatatctt taggcttaat
    18781 caagagaatt tcgttggcta aagacaactt cttaagacga atcgctgtgc ggacgatatt
    18841 ttcaatatca aatcctgtca agacgatacg tcgacctgtt ttatccgcag cgtcaaaaat
    18901 ctgctgaata cgagaaagat tactggaaac agctgcaacg atgatacgac cttcccagtc
    18961 agcaatagtt tgggtaattt catccctaac ttcactttca ctagccacct gaatattgct
    19021 gtctgcattg gccgaatcac tgaggagagc caggacgccg tcacgaccaa tctctgccaa
    19081 acgagcaaaa tcagttgcat aagattcact agccgtttgg tcaaatttga agtcacctgt
    19141 ataaacgatg cttccttccg atgtcttcaa gacaattccc agactctctg gaacggagta
    19201 agtcgtaggg aagaaggaaa ccactgtccc accaaaatca atctccgtat tctcatcaat
    19261 gacatggaaa tcattaaatt tcttaacggc atcatttcct ttgacaaaga gctttgccaa
    19321 ctcaatggtc aactcagacc caaatacagg aactttagcc tctgccaaga gatacggtag
    19381 agcaccaatg gcatccgcat gcccgtgggt caagaaaacc ccagcaatac ggtcgctatt
    19441 ttcaaaaagg taatccatgt ttggaatcac cacatcgacc cctaattgtt cattttcagg
    19501 atattttaac cctacattca aaacaaaaat ggactctcca atttcagcaa tgtacatatt
    19561 ttttccattc tcacgcacac caccaagtgt tgttaaactg atattactca tttttcctcc
    19621 aaaatcttag tttatcttga ttatagggca attctaccct cttattctaa aagttctgat
    19681 actttcagcc gaatcttttc ctaccattat accatttttt tgatgatttt caaaatgaag
    19741 gtctgttttc aaaaaagagt tgatttccca actctttcct aacttctact ctttttccat
    19801 caaaaaatca taaatttctt gcaccgtacc cagcgccatc atttcttggt ctttaacggt
    19861 ttgtttgaga ttttggtaaa tttgactttc tcctatctcc cgctttggcg cctctttatt
    19921 gactgtcatc acaccatctt ttatcgtcac aaagcctagt tcttcaaata cttgaatcat
    19981 cttgaccagc aagatttgtt gaatattaag atatgcagcc aaatctttca gcttgtagcg
    20041 aatatcaaac tctgggaact ggtaaatagt cttgtacaat ttggcaaact gatctctagt
    20101 cccataacct gtcagataat aagccttgtc aatatcattt ttgaaataga cagcagagaa
    20161 atgctgttcc tgaaaaatgg tcttcagctg agtaatatcc tctggaatgt tttttacgac
    20221 aacagcttca ctagccgcaa gatttggcag ttctccagga aaatccaaga ctggaacacc
    20281 ttctggcaag actgcatttt ttccacgaat gttaaaaagt tgaacacctt ccactcgcgc
    20341 atccaccatc atcaactgga gggcagtttg gccattccat tggttgacag acaatttaac
    20401 cgctaactct agattcttgg tttgagaaaa ctctgtcgcc catctgcctt gaccaaaggc
    20461 taccacttca aaactcgcct cacccttgga aattttcagc tttagatggg cattacctgc
    20521 ccccatagta cgagcacttt cgacctgaaa attcttgata taaaaaatag gtttctgatt
    20581 atccattcca aaaggagcta aacgttcaaa acttttgacc gtttccaagc taagtgcctc
    20641 caaatccaac tcttcatcta ggtttaactt attcttgcca ccagcatctg cacctttttc
    20701 acgaacataa tcttccaaaa cctgagataa atctgagagt tgctcaactt ccaacgtcat
    20761 acccgctgca cctgcatgac ctccaaaggc gatgaagagg tctcgatggg gatccagagc
    20821 ttcaaaaata tcgaccgctt ccacactacg agcactgccc ttggcacgac cgtcttctat
    20881 attaagaaca atgactgtct gtcccaattc ttccaataaa cgaccagcca cgattcctag
    20941 aaccccagga ttccagcctt ccttggccaa gacctgaacc ttcttctcag gatccaccat
    21001 ggtcttggct tcttcataga tagactgaac gatttccttg cgctcttcgt ttttctggtg
    21061 aatcataagg gcaatctcat gcgcttcctc atcatcaaat ccagtcaaca aatcaatggc
    21121 aggattggga tcatccaagc gacccaaggc attcaaacga ggagcaatct ggaaaccaac
    21181 cgtttcttct gttacttcgt tggcaccaat cccagccatg tccagcattt cttgcagacc
    21241 aatgcgctgg gtatgaccca acatttccag accatattga actaagatac gattttcatc
    21301 cgtcagactc accatatctg caatagttcc aatagcgacc aaatcaagca attccacttg
    21361 cacttcttct aacagggcac aagccaactt gaaagcaact ccacaaccag ccaaatattt
    21421 aaaaggataa tccgcatctg gatgttcagg atggacaata gcataagcat ctggcagggt
    21481 ttcaggcatg gaatgatggt ctgtgacaat gacatctact cccatagact gagccaatgc
    21541 aatagcctca tgaccagcaa ccccattgtc caccgtcaca atcaaggaaa tcccttcttg
    21601 ctcgataaag tatttataaa cactagcatt agggccatag ccatcggtaa aacgatttgg
    21661 caggtaaact cggcactcag caccaagttg ttccaaactt tccttcacaa tagaagccga
    21721 agtcatgcca tccgcatcat agtctccata aacgagaata ttttcccctt cttcaatagc
    21781 ctgacgaatc cgctccactg ccttgtccat atcatggagc agataagcat catgtaagtc
    21841 ctccaaggaa ggttctaaaa acttcttcag actttcttgg tcctgaatcc ctctctcaaa
    21901 caataaccga gccacctcag gacccagtcc agccttcttg gctatctttg taaaatccgc
    21961 atcttctacc tgcagggcaa actgccattc ataagtaggt gttatcaaaa agacatccac
    22021 tctttctaag aattctattg cttcattata acatgattta gacgttttct ctatccagat
    22081 tgctttttta taacatttta tagtatattg aaactagaat agtacacctc tacttctaaa
    22141 atattgttag aaatcgattt gactgtcccg atcgatttgt cctgttctta tttcactata
    22201 gtgaattttt tcagatatgg tttgacaaga acattctttt attgtagaat cgtgttataa
    22261 aatctaacat aaaggagatc tctcatggct ttagtagaat ttaaaaacgt cgaaaaatat
    22321 tacggagact accacgcact ccgcaacatc aatctccgtt ttgaaaaagg acaagttgtt
    22381 gtcctgcttg gaccttctgg ctctgggaag tccactctta tccgtacgat caatggttta
    22441 gaggctgttg acaaaggaag tctcctagtc aatgggcacc aagttgctgg tgccagccag
    22501 aaagatttgg tacctcttcg caaggaagtc ggcatggttt ttcaacattt taacctttat
    22561 acacacaaaa ctgtgttaga aaacgtgaca cttgcgcccg ttaaagttct aggaattgat
    22621 aaaaaagaag ctgaaaaaac cgcccaaaaa tatctggaat ttgtaaatat gtgggacaag
    22681 aaagattcct atcccgccat gctatctggt ggacaaaaac agcggatcgc catcgctcgt
    22741 ggtcttgcta tgcatccgga actcctcctc tttgatgaac caacatctgc tcttgatcct
    22801 gagactatcg gagatgttct agcagttatg cagaaactgg cgcatgatgg gatgaacatg
    22861 atcatcgtta cccacgaaat gggctttgct cgagaggttg cggaccgcat tatctttatg
    22921 gccgacggag aagtgttagt agatacgaca gatgtcgata acttttttga caatccaagc
    22981 gaacctcgtg cccaacaatt cctcagcaaa attatcaacc acgaaagtga caaagtcaaa
    23041 taaggaggcg cctatgaaaa aaaaattctt tttatcagca ttattgatta gccttttcgg
    23101 ccttgctgct gccaaaccag tccaggctga tactagtatc gcagacattc aaaaaagagg
    23161 cgaactggtt gtcggtgtca aacaagacgt tcccaatttt ggttacaaag atcccaagac
    23221 cggtacttat tctggtatcg aaaccgactt ggccaagatg gtagctgatg aactcaaggt
    23281 caagattcgc tatgtgccgg ttacagcaca aacccgcggc ccccttctag acaatgaaca
    23341 ggtcgatatg gatatcgcga cctttaccat cacggacgaa cgcaaaaaac tctacaactt
    23401 taccagtccc tactacacag acgcttctgg atttttggtc aataaatctg ccaaaatcaa
    23461 aaagattgag gacctaaacg gcaaaaccat cggagtcgcc caaggctcca tcacccaacg
    23521 cctgattact gaactgggta aaaagaaagg tctgaagttt aaattcgtcg aacttggttc
    23581 ctacccagaa ttgatcactt cccttcacgc tcatcgtatc gatgcctttt ccgttgaccg
    23641 ctctattcta tctggctaca ctagtaaacg gacagcacta ctagatgata gtttcaagcc
    23701 atctgactac ggtattgtta ccaagaaatc aaatacagag ttcaacgact atcttgataa
    23761 cttggttact aaatggagca aggatggtag tttgcagaaa ctttatgacc gttacaagct
    23821 caaaccatct agtcataccg cagattaagg aggataccct atgacagatt tatcatcttg
    23881 gacagcctat tttcaggatt ttggacaatt tttcaatggt ttcctcttta ctcttgccct
    23941 agcgattgga tcctttatcc tcgccatggt cttaggaatc ttctttggtg ccatgtcaac
    24001 cagtaaacgt ccgattttgc gaatcttagc tcgcatcttc gttgaatttt accaaaatac
    24061 tcccctcttg gtgcagtttg ttattgtttt ttatggccta cctcttatca gtgaccatac
    24121 tatcatgatt ccgatttatt ggacagctgt tctctgcgtg ggactctatc acggcgctta
    24181 tatcgctgag gttattcgtt cagggattca gtctattcct agtggtcaga tggaggccgc
    24241 cttgtcgcaa ggttttacct atatcagtgc catgcgcttg attatcttgc ctcaggcctt
    24301 ccgcatcatt ctccctccat tgaccaacca aattgttaac ctcatcaaga acacctctac
    24361 tgtagctatc atctctggag tagacttgat gtttgtgact aagtcttggt cggctctcaa
    24421 cggaaactat atcccaggct ttttaggtgc tgctcttctc tactttgccc tatgcttccc
    24481 tgttgcccag tttggtcgca agatggagca agtcaacaaa aaagcctatt cactttagga
    24541 ggttactatg gaatctattt tagaagtttt aaccccagat aacctagtct ttatctttaa
    24601 aggatttggc ttgaccctct atatttctct gattgccatc atcctctcta ctatcatcgg
    24661 taccgtgctt gccgtcatgc gaaatggaaa aaatcctatc ttacgcatta tttccagtat
    24721 ttatatcgag tttgtgcgca acgttcccaa ccttctctgg atttttacta tctttttggt
    24781 gttcaaaatg aagtccacac cagcaggtat tacagccttt actctcttta catcagcagc
    24841 cttggctgag attattcgag gcggtctcaa tgccgtagac aagggacaat acgaagcagg
    24901 aatgtcacaa ggcttcacct cagcccaaat cctctactac atcattctcc cacaagccat
    24961 ccgcaaaatg ctaccagcca tcatttctca gtttgttacc gtgattaagg ataccagtct
    25021 cctctactct gttatcgccc tacaagaact ctttggagcc agccaaattc tcatgggccg
    25081 ttatttcgaa ccagagcagg tcttcagtct ttacatcctg attgccctca tctacttcag
    25141 ctttaaccta gcaatttcta acctgtctca tatgctagca aaacgttggc aacaagctgc
    25201 agaataacaa attagctctc tagttcattg gagagttttt tgatgatagt agatatttat
    25261 agacctcaat ttgacaccat acattttcta tgataaaatg taacaaattt atttacaaca
    25321 aatcgaaatc taaatgaaat ggaattttct atgaaatcaa tcaaaggact actctttatc
    25381 atagctagtt ttatcttgac tcttttgact tggatgaaca cttctcccca attcatgatt
    25441 ccaggactag ctttaacaag cctatctctg acttttatcc tagccactcg tctcccacta
    25501 ctagaaagct ggtttcacgg tttggagaag gtctacaccg tccacaaatt cacagccttt
    25561 ctctcaatca tcctactaat ctttcataac tttagtatgg gcggtttgtg gggctctcgc
    25621 ttagctgctc agtttggcaa tcttgccatc tatatctttg ccagcatcat ccttgtcgcc
    25681 tatttaggca aatacatcca atacgaagct tggcgatgga ttcaccgcct ggtttaccta
    25741 gcctatattt taggactctt tcacatctac atgataatgg gcaatcgtct ccttacattt
    25801 aatcttctaa gttttcttgt tggtagctat gcccttttag gcttactagc tggtttttat
    25861 atcatttttc tatatcaaaa gatttccttc ccctatctag ggaaaattac ccatctcaaa
    25921 cgcttaaatc acgatactag agaaattcaa atccatctta gcagaccttt caactatcaa
    25981 tcaggacaat ttgcctttct aaagattttc caagaaggct ttgaaagtgc tccgcatccc
    26041 ttttctatct caggaggtca tggtcaaact ctttacttta ctgttaaaaa ttcaggcgac
    26101 cataccaaga atatctatga taatcttcaa gccggcagca aagtaaccct agacagagct
    26161 tacggacaca tgatcataga agaaggacga gaaaatcagg tttggattgc tggaggtatt
    26221 gggattaccc ccttcatctc ttacatccgt gaacatccta ttttagataa acaggttcac
    26281 ttctactata gcttccgtgg agatgaaaat gcagtctacc tagatttact ccgtaactat
    26341 gctcagaaaa atcctaattt tgaactccat ctaatcgaca gtacgaaaga cggctatctt
    26401 aattttgaac aaaaagaagt gcccgaacat gcaaccgtct atatgtgtgg tcctatttct
    26461 atgatgaagg cacttgccaa acagattaag aaacaaaatc caaaaacaga gcttatttac
    26521 gaaggattca agttcaaata agtaatcata ctctcctggg atttcccagg agagtttttt
    26581 atagccaaat gaaataagac cggaacgatg gaattttggc attcaaatca attgataaga
    26641 atattttata gtggagtgaa ataggatacg aacaaattga ttaggaaaat caaatgaatt
    26701 tatagaaatc ttttagcagt tatgttgtcc tattctagtt tcaaaacgct atggaagcag
    26761 cattgtgcta gtctagattc agtttactat actaaaacga gtagcttgaa atcaaaaaac
    26821 ccaccctcac aggcaggttt tatctgtatt attcagctag attatgcttt accttctgaa
    26881 ccgaatacgt cgatacgttc ttcaaccgat gcttggatag cttttacacc gtcagccaag
    26941 aatttacgtg ggtcgaagag ttttttcttg tcgtattctg cttcgtttgc ttcgtaatca
    27001 cgagcaaatt tacgagttgc gttagcgaat gcgatttggc attctgtgtt aacgttaact
    27061 ttggcaacac caagtttgat agctgcttgg atttgctcat caggaatacc tgatccaccg
    27121 tgcaatacga ttgggaatcc tggaagagct tctgtcaatt tttgcaagtg gtcaaggtca
    27181 agaccttccc agtttactgg gtaagggccg tggatgttac cgataccagc tgccaagaag
    27241 tcgataccag tttcaaccat tgctttagcg tcttcgattg gagccaattc acctttaccg
    27301 atgattccgt cttcttcacc accgatagta ccaacttcag cttctactga gataccttta
    27361 gcgtgtgctt tttcaacaac ttctttagcc aatttaaggt tttcttcaac tggaaggtgt
    27421 gaaccgtcaa acatgattga agtataacca acttcgatac actcaagtgc atcttcgtag
    27481 tgaccgtggt caaggtggat agctactggt acagtgatac ccattgattc aacaaggtta
    27541 gcgatcaagt tgcgagcaac tttgtaacca cccatgtatt tagcagcacc cattgaagtt
    27601 tggatcaaaa ctggagcttt tttagcttct gctgcgcgca agatagcttg agtccactca
    27661 aggttgtttg tgttaaatcc accaactgca taaccgttgt cacgggctgc ttggacaaat
    27721 ttttctgctg aaacgattgc cattttatca ggcctcctgt atatttttat gggtcatccc
    27781 atttacattg ttcattttat cactttttgc caaaaaaatc tagtttttcc cgcagtttcg
    27841 attgattttc ttctaactcc atctatgtaa accctttctc tccctagtct tggacgactt
    27901 ttggaaaagc tataaagaag gttaaacgat tctcctccat ctcgaaacga taagctaatt
    27961 tttcatgttc taatagactc ttaaccacaa aaagccccat accagacccc ttgaccttgc
    28021 gactggcatt gtcagaaaaa gactgggcta gtttttcttg ttcctctgag ctacagctat
    28081 tttcgataaa aagttctcct tctctttctc caattcgaac taagccacct ggaacagagt
    28141 gcttaatggc attgctgatg agattagaaa gaatcaactt cataactgat gggtttagat
    28201 aagcctgctg atgggtcaaa ctattgtcta tctggagctc tctttccttg gctagcaagg
    28261 cataatcttt gaccagattt ttcgtcatct ggaggaggtc aattgtttcc ctatcatctc
    28321 gcaattcctg cacagaagag agggaaagta tctgcagaac atggtgattg agttcatcca
    28381 caatccccaa ggcaactccc agatactggt ctctatcctt ataacgaccg atattctctc
    28441 tcatattttc gattaggatt ttcaaactag ccagcggtgt tttcaattca tgagaagctc
    28501 ctcgtaggaa ttcgaccttc atcttctcca gctggagaat ggcttcattc ttttcatgca
    28561 agtccgcaat aacagtcaag agatgctggt agaggctatt gatttgttcc ttgagattac
    28621 ctatctcatc cttagaatcc acgcgcaatc gcacttggga atccaggtcc atcatccgac
    28681 gggtcacccg cttgatttcc aaaatcggtg caacaatagt ccgagcgtag atgtaggcca
    28741 ccaagaggga aatcagaaag gaggccagca aggtataggg aagaaactgg agactgattt
    28801 gctccgcttc cttttgtaaa tccatggaag ctagaaactg gagaatcata gtaccaccgt
    28861 cttgcgtttt cacctcgcgc tcctcaataa agagagaggt tgtctggcgg tctgtgtcca
    28921 gaggaagact gtccttgact tctaacttgt cctcggtcat ctcacctttg acggtcccct
    28981 tgatatcact agtctgggaa tacaagtcta acacttgctc gatactctgc ctatctttcc
    29041 cttctaggga ctgggcaatg gctgttgcct tttgaccaat ggtttcctga cgatgactca
    29101 gataagtcga aggaaaaaga aaataaatag ctaaatgaag gcagataacc agaacactaa
    29161 atatcgagaa ggtatagata aatatctttg taaataaacc tgttcgtttc attttcgctc
    29221 caatttataa ccaacattgc gcacagtgaa gatacaatcc aagtctagct ttttccgcaa
    29281 ttccttgata taaacatcaa taacacggtc aaagggaacc tcatctgtcg ctttccagac
    29341 ggcatcgata atctgagacc gagtcaaggc ccggccttca tttttcacta gatagtccag
    29401 aatttccaac tctttggcat tgataggcac ttcttgacct gcgaggcttg cactgtagct
    29461 ttcaaagtcc accttggtat ccttgtaaga aaagattcgt cctgtatcgt agtagcgctt
    29521 gaaaatcgcg tccaccctca cttttaagag ggagagggag aaaggttttt ccagatagcc
    29581 atctgccaaa gaggcaaagg cactcatctt gtattcctca tcctgaaaag ctgtcaacat
    29641 caagacagga acctgactgg ttttacgaat ctcagctagg acttctaagc cgttaagctt
    29701 gggcatctgg atatccagta aaaccagggc cacctcatag ctagaaaatt gctccagagc
    29761 ttcctgaccg tccgctgcct caatagtttc atagccacaa tccgtcaaat aatcactgac
    29821 cccctcacgg atcatctctt catcttctac aattaaaatt ttcatacttt aactgctctc
    29881 tattttttat ttttcttaga ataaatacct actctatttt ctattatagt ctcttgctgg
    29941 ccttttgtct gtaagcaact gaccactaga taaaacgttg tgaaattcct ttctcataaa
    30001 ttccataact ttagtatatt atatttaagc actaaagtac aaagaaagca actgaaagca
    30061 atgattttca ccactgcttt cgatttattt tgaattgtta aatagctatt cctatccact
    30121 attcttgaat agaaacacaa gatgcaatct ttattccaga ctcatttttt caaatttatt
    30181 caccatccag caagagctct tttggttgtt ttctaaggag attgcttgaa gcaagcgcca
    30241 taacgagaac cactagaacc aaggcaagga caaaaatgat gataaagtct gatgtctgaa
    30301 tggaaatgtc taggctcgac aaggtcttgc taaagccatc tacttctgca ccgccaccaa
    30361 ggttagaggc ttgagccgcc ttactagcct gtttggcaac acctgaagtc acattggcaa
    30421 ggacagtgtt tccaattgca cgggcagtgt aattagctag gaagtaagca gaaactagag
    30481 cagggatagc aatcaagata gattcggtta tgaattgacc caagatactt gcctgcttga
    30541 ggccgataga gaggagaatt cccacttcct tgcgacgggc gttgatccaa aggctgagca
    30601 agagggcaag gaggagaact gagaagctca agctacccca gaagaggagg ttggccatct
    30661 tgtacatacc agagatagat tgctcaagag ctgggtagtt agaggagctc ttgacgagtg
    30721 tgtagctctt ccagttgata ccactgatgc cattcaactc tttcataaca tcatccaagt
    30781 tcttgtctgc tgttacaaag aaggttgcgt ccccataaat ggctgtgtct tctgtgtatc
    30841 cataaagttt tgcagcagtg tgaatgtctg taatagctgt gttttcgtaa agttcttgtg
    30901 agtaggttac tgctgactta ttatgaccat caaagagtcc cttgattgtc acttcaactg
    30961 tttccttggc tcctttttca ttatctgcat cgtagatatt agagtccagt ttaaccttgt
    31021 cccctacttt ccagccgtgt ttggctgcca agtccttgtg caagaggatt ttatccttgt
    31081 cgtcgttggt taagtgctct ccttcgacta gtttataaga accagagaca aacttgtctt
    31141 ctttagagga gtcattgaca cctgtaatca tcaagctact tccaaaacgt ttggcacgat
    31201 cagcagtgag attcttcttg gtttctggcg tttcaatcag gtcatatcca gtcaaatctc
    31261 cgatagcgtt gatacgtttg acataagact caatggcctt gttttcggtg atttttttga
    31321 tgtcttcacc cttgatattc ccagcaccac gaggcgttcc ttggttgacg cgacgattga
    31381 tttgcatgga gaagctattg gtgatatttt taaaggtctc ctgagaagcc ttggcagtag
    31441 ctcccttgat tgacaagccg accaaactca agctcgccat aaggagaata atcaggaaga
    31501 tgacaatcga tttgaaaaac ttccttgtaa cataggcaaa tgcgttgtgt aacatagatt
    31561 ccctttctag attttgtttt aatcattcta ttaaaataag ctcaaattat ttactagtat
    31621 tgcgcgtttc agtcagtttc ttatccttta attcaagtgt aatatctgac gcttgtgcca
    31681 cttctttact gtgagttacg acaatcacac atttacctgt tttctgggca agtgatttga
    31741 gtagttcgac aatatctcca gcagttttag gatccagatt tcctgttggc tcatcagcta
    31801 gaataactgg agcttctgag accaaactgc gagcaatggc aacacgttgc tgttgaccac
    31861 ctgataactg gagaacattc cgcttgatct ggctttcatc caaaccaagc tcaagaagtg
    31921 tatccttgct tgcctttttg ttgaccaagc ggatattttc cagcggagaa agataatcta
    31981 tcaagttata attttgaaag accagggaaa tatggtgcat gcgatggtaa gaatagccct
    32041 tcttacgaat atcctctcct tgaaaaagga tagaaccttc aacaggacta tctagaccag
    32101 caagtaggga caagagtgtg gattttcctg ctcctgactc cccaataata ctgtaaaatt
    32161 ttccgggttc aaaattataa tcgatctgat ataggactgc ttcagcagta ttcttataac
    32221 ggtaggtaac atcttgtaat tgtaataaag tcatgatttc tccttcttaa ctaatagatg
    32281 ataaaatttc tttcggtgat tttctaaata agaataggaa acaaagggct acagataagc
    32341 aactaagcag aactagaaaa acataggatt ctgcaaaaga taagatgcta gttgataaac
    32401 tgcttgcttt ggctagtgta tcttgtaagc ttgcctgatc tccacttgct agtagagttt
    32461 ggagtaggta agttgtgatt ccgtttcctg caacaaatgc tggaagcaaa gctccaagag
    32521 ataccaaaac tacctctaaa cagaattgta ggaagatcga gctcttgcct tttccaagtg
    32581 caagtaaaat ccccacttca tagacccgtt ctctcaacca gagagacaaa accagaatta
    32641 aggctccagc tcctgctatc aacatcccat aaaggaagat ggtcaggaag gtttggaaag
    32701 ttgcaactga gtctttgatt tgttcaaaag ccttgttttc cttttcgact tggtagcctt
    32761 gattttccaa ggccaagttt tctacctgct tcatgagtcc gtccatttcc ttaggatttt
    32821 ctacatagaa gcgtgctgca ctgacttgag cttcactatt gcccaaaagg gtttggctac
    32881 tgtcatagtc tgtaaagact tgattttcac tgaagtcaga agacaagcct gtgaatttct
    32941 cttgtttttt accagaaaag atgccgataa tctcaaactc tactgtttgt ccttttccag
    33001 attcagactg accagcatcc aagccaatct tgtcatgaag cgaaagaccg ttcttcttag
    33061 ccaattcttc gtggataagg attttcttgg aatccccttt ttgaaggtgt cgcccttctt
    33121 ttagattgaa agccgaactg gtaaaggtta catccttgga tgaatcctca agagccgtta
    33181 agctaaccaa gttattgtct gcagctgata aatcatcacg ctccacgctc tgctcgccag
    33241 tcactgcttc cttgtctttt agttttgcga ccgtctcaag ttcaggagag acattttcca
    33301 gccccttaat cttgcttaca gatgctaggt ctgacaactt gaatgtctga ccattctcta
    33361 tcttcttaat agaaaaagat gtattgagtg atttataaag attgctttct actgttttgt
    33421 tggacttcat cagagtcaaa caggctgaaa ttccggccaa taagaccaat aaaatcagaa
    33481 ataaaataaa acttctcagt cgctttctgc tgacataagc ccaagatctt tggattggat
    33541 tcatttgtca cctccatatt tgtaagacta ttataaaacc caaatatgaa atgtttatga
    33601 aatacgaaaa aaaaatatcg agtaggggat aatctctagc ccctctcaca ccacc
//
LOCUS       DAVHIT010000015        32656 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVHIT010000015 DAVHIT010000000
VERSION     DAVHIT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.015"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            29..2953
                     /locus_tag="VVN16_000569"
     CDS             29..2953
                     /locus_tag="VVN16_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5572012.1"
                     /translation="MTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETG
                     DVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGR
                     DGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGED
                     GKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGN
                     PSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVY
                     QEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTT
                     VFVQDGAKGETGAIGQDGQTPTITTQRGQDGQSTVVTITTPGKDPVTFTVKDGKNGKD
                     GRAPKIKVEDITSPSRIRRATDAAATPTRNGIRVTVYDDVNDNGVYDGGVDKVLNSKD
                     IYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENP
                     DGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIK
                     DGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDG
                     SHTIVIKNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTETIIKDGKSP
                     VATVRDNQDGTYTIRVENGNGTVSETTVRDGKSPTAKVVDNGDGTHTITVVNSDGTTT
                     TTTVRDGREYKSIIKDGKDGKDGVSPTVTVENNNDGTHVVTITNPDGSKTEMVIKDGK
                     DGKSPKVSVEDNGDGSHTITIINSDGTVTKTVIKDGKDGRDGRDGRDGKDGKCGCQDK
                     PVTPSNDKPVPPAPNVPTPEVLVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVPEQP
                     VVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRK
                     ED"
     gene            complement(3190..3738)
                     /locus_tag="VVN16_000570"
     CDS             complement(3190..3738)
                     /locus_tag="VVN16_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5572013.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGASALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            4059..4547
                     /locus_tag="VVN16_000571"
     CDS             4059..4547
                     /locus_tag="VVN16_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU5572014.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            4544..5731
                     /locus_tag="VVN16_000572"
     CDS             4544..5731
                     /locus_tag="VVN16_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5572015.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEVA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEESEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            5724..5948
                     /locus_tag="VVN16_000573"
     CDS             5724..5948
                     /locus_tag="VVN16_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU5572016.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            6105..7451
                     /gene="asnS"
                     /locus_tag="VVN16_000574"
     CDS             6105..7451
                     /gene="asnS"
                     /locus_tag="VVN16_000574"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU5572017.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            7604..7894
                     /locus_tag="VVN16_000575"
     CDS             7604..7894
                     /locus_tag="VVN16_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5572018.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            7906..8376
                     /gene="ssbA"
                     /locus_tag="VVN16_000576"
     CDS             7906..8376
                     /gene="ssbA"
                     /locus_tag="VVN16_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU5572019.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            8408..8647
                     /locus_tag="VVN16_000577"
     CDS             8408..8647
                     /locus_tag="VVN16_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5572020.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            8942..10342
                     /locus_tag="VVN16_000578"
     CDS             8942..10342
                     /locus_tag="VVN16_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU5572021.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIGKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            10344..10703
                     /locus_tag="VVN16_000579"
     CDS             10344..10703
                     /locus_tag="VVN16_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5572022.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHYLKKKK"
     gene            10757..11506
                     /locus_tag="VVN16_000580"
     CDS             10757..11506
                     /locus_tag="VVN16_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU5572023.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            11496..11795
                     /locus_tag="VVN16_000581"
     CDS             11496..11795
                     /locus_tag="VVN16_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5572024.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            11866..12801
                     /locus_tag="VVN16_000582"
     CDS             11866..12801
                     /locus_tag="VVN16_000582"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU5572025.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            12888..13400
                     /locus_tag="VVN16_000583"
     CDS             12888..13400
                     /locus_tag="VVN16_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5572026.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAAFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERDLHDSDF"
     gene            13425..14033
                     /locus_tag="VVN16_000584"
     CDS             13425..14033
                     /locus_tag="VVN16_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU5572027.1"
                     /translation="MFGELIRYLDQYEDVILREIKSQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            14134..14328
                     /locus_tag="VVN16_000585"
     CDS             14134..14328
                     /locus_tag="VVN16_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5572028.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(14374..15819)
                     /locus_tag="VVN16_000586"
     CDS             complement(14374..15819)
                     /locus_tag="VVN16_000586"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU5572029.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKILAFTGTKGKTTAAYFAYNILSQRYPTALLSTMNTTLDGTTFFKSSFS
                     TPENIDLFDMMAQAVKNGRSHLVMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            15925..17547
                     /locus_tag="VVN16_000587"
     CDS             15925..17547
                     /locus_tag="VVN16_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU5572030.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            17748..19709
                     /locus_tag="VVN16_000588"
     CDS             17748..19709
                     /locus_tag="VVN16_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5572031.1"
                     /translation="MKKSKSKYLTLAGFVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRATTNDIVTNLVDGLMENDQYGNYVPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGI
                     KALDDQTVQYTLARPEPYWNSKTTSTILFPVNADFLKSKGDDFGKVDPANILYSGPFL
                     MKAFVSKSVIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQDALVRNFTDGAYSYARLYP
                     NSSSFEGIKEKYKDDIIYSMQDATSYYWNFNLDRQAYKFTSKTTDIEKKSTQEAVLNK
                     NFRQAINFAYDRTSYGAQTQGEDGATKILRNLVVPPTFVSIKGKDFGEVVASKMVNNY
                     GKEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDATVDQASKKGIQE
                     VSSMKQSIEAALGTENVVIDIQQLSTEDYDNSSYLAQTAIQKDYDLYNGGWSPDYLDP
                     STYLDIFSVKNGGVLQNLGLDPGEANDKAKAVGLDTYTQMLEEANKEQDPAKRYEKYA
                     DIQAWLIDSSLAIPNVSLGGTPSLRKTVPFSAPYSLAGNKGVESYKYLKVQDKIVTTD
                     EYAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            19881..20114
                     /locus_tag="VVN16_000589"
     CDS             19881..20114
                     /locus_tag="VVN16_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU5572032.1"
                     /translation="MKLKLLRVDTKVIMWSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            20120..21562
                     /locus_tag="VVN16_000590"
     CDS             20120..21562
                     /locus_tag="VVN16_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572033.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIYSSSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIART
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            21720..22814
                     /locus_tag="VVN16_000591"
     CDS             21720..22814
                     /locus_tag="VVN16_000591"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5572034.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            22822..23988
                     /locus_tag="VVN16_000592"
     CDS             22822..23988
                     /locus_tag="VVN16_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5572035.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNCGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            24081..27179
                     /locus_tag="VVN16_000593"
     CDS             24081..27179
                     /locus_tag="VVN16_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5572036.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            27227..27844
                     /locus_tag="VVN16_000594"
     CDS             27227..27844
                     /locus_tag="VVN16_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5572037.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            27855..29189
                     /locus_tag="VVN16_000595"
     CDS             27855..29189
                     /locus_tag="VVN16_000595"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU5572038.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNWTSNVQ"
     gene            29199..29663
                     /locus_tag="VVN16_000596"
     CDS             29199..29663
                     /locus_tag="VVN16_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572039.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            29660..30154
                     /locus_tag="VVN16_000597"
     CDS             29660..30154
                     /locus_tag="VVN16_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572040.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            30235..31890
                     /gene="mltG"
                     /locus_tag="VVN16_000598"
     CDS             30235..31890
                     /gene="mltG"
                     /locus_tag="VVN16_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5572041.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADESQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATEIVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            31961..32443
                     /gene="greA"
                     /locus_tag="VVN16_000599"
     CDS             31961..32443
                     /gene="greA"
                     /locus_tag="VVN16_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5572042.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(32500..>32656)
                     /locus_tag="VVN16_000600"
     CDS             complement(32500..>32656)
                     /locus_tag="VVN16_000600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572043.1"
                     /translation="EVSSKHGFEVVDETDEVSSKHGFEVVDETDKVSNHTYGKATLTW
                     FEEIFEE"
BASE COUNT        10001 a         6091 c         7355 g         9209 t
ORIGIN      
        1 aacggttcat aatccaggaa gtccagatgt gacaactacg atccgtgatg gagctacagg
       61 acaagcaggt cgtgatggta aagatgtatt aaacggaaaa gtaaatccac aaccaaacca
      121 aggtaaaaat ggagataaat atattaatac cgaaaccggt gatgtctatg ttaaaaacaa
      181 tggaaactgg gataaagaag gcaacatcaa aggccctaaa ggggacaaag gtgcagatgg
      241 tgctaaaggc gaaaaaggag accaaggcga acgcggccta actggagcgc aaggagctaa
      301 aggtgcggat ggcgcagtag gtcgtgatgg acgtgacggt aaagacgtgt tgaacggcaa
      361 agctaaccca gaagcacatc aaggtaaaga cggcgataaa tacgttaata cagaaacagg
      421 cgacgtcttc gttaagaata acggcaactg ggataaagag ggcaacatca aaggccctaa
      481 gggtgacaaa ggtgaacgcg gagaagatgg taagactcca gaagtaactg taactccagg
      541 taaagatggc catagtactg acattacatt cactgttcca ggtaaagatc cagttacagt
      601 aaatgttaag gacggagaaa atggtctgaa cggtaaaact ccaaaagttg atttacttcg
      661 tgtccaagga aaaaacggaa atccatctca tacaattgtg acattctata cagatgaaaa
      721 caatgacggc aaatatacac caggaactga tgaacttcta ggttcagaaa tgattaaaga
      781 tggtgctaaa ggcgcggacg gacgagatgg taaatcattg cttactgtca aggatggtaa
      841 agaaactaaa gtttaccaag aagatccagc taacccagga caaccattaa atccagaaaa
      901 accacttgcg gtaattagag atggagtaga tggaaaatca cctacagtta cagctgttcg
      961 taaagatgaa gcagggcata aaggtgtaga aatcactgtt gataaccatg atggttcaca
     1021 accaactaca gtctttgttc aagatggtgc taagggagaa actggtgcaa tcggtcagga
     1081 tggacaaact cctacaatca ctactcaacg tggacaagat ggccaaagca ctgttgtaac
     1141 tatcacaaca ccaggtaaag atccagtaac cttcactgta aaagatggta agaatggtaa
     1201 agatggccgt gcaccgaaaa tcaaagtaga agatattact tcaccttcaa gaattagacg
     1261 cgctacagat gctgctgcaa ctccaacgcg taacggtatc cgtgttacag tttatgatga
     1321 tgttaatgac aatggggtat acgacggagg tgtcgataaa gtattaaata gtaaagatat
     1381 ttataacggt atagatggac gtgatggttc agctccaact attactacaa aagataatgg
     1441 agatggaaca cacactatca cagttcaaaa tcctgatggt tctgaatcaa caactgttgt
     1501 taaagatggt aaagacggta aaactgcgaa tatcactaca acagaaaacc cagatggaag
     1561 ccacacaatt acggtgacaa atccagatgg ttcaactaaa gaaactgttg ttaaaaacgg
     1621 taaagacggt aagactccta aagttgaagt aacggataac aacgatggaa ctcacacagt
     1681 taaagtgacg gatggagacg gtaatgtaac gaacgctatc atcaaagatg gtaaagatgg
     1741 taaagctgca acagcaacaa ctactgaaaa tccagatgga agccacacag taacaatcac
     1801 taacccagac ggaactaaga atgagtttgt tgttaagaat ggacgtgacg gtgttgacgg
     1861 acgtactcca accgcatctg ttcgtgataa tggagacgga agtcatacaa tcgttattaa
     1921 aaatccagaa ggtgtgacaa ctgaaaccac agttcgtgat ggtaaatcac caaaagtgac
     1981 tataactgat gaacaaaatg gaactcataa gatctctgtt ctaaatggtg acggaacaac
     2041 tactgaaaca atcattaaag atggtaaatc accagtagca acagttagag ataaccaaga
     2101 tggtacttac actattcgtg tggaaaatgg taatggtact gtttctgaaa ccacagttcg
     2161 tgacggtaaa tcaccaactg ctaaggttgt ggataacgga gatggaacac acactatcac
     2221 agttgtgaac tcagacggaa caactacaac aactacagtt cgtgatggtc gtgaatacaa
     2281 atctattatt aaagatggta aagacggcaa agatggcgtt tcaccaactg taactgttga
     2341 aaataataac gatggaactc acgttgttac aatcactaat ccagatggaa gtaagacaga
     2401 aatggtgatt aaagacggta aagatggtaa atcaccaaaa gtttctgttg aagataatgg
     2461 tgatggtagt catacaatca caatcatcaa ttctgatgga actgtgacaa aaacagttat
     2521 taaagatggc aaagatggta gagatggacg tgatggtaga gacggcaaag atggaaaatg
     2581 tggatgccaa gacaaaccag taacaccatc aaatgacaaa ccagtacctc cagcaccaaa
     2641 tgtgccgaca ccagaagtac tggttaaacc tgtgccagcg caaccaacac caaatgtacc
     2701 gacaccagaa gtgccagtac aaccaactcc agctgttcca acaccagaag taccggttcc
     2761 agaacaacca gtagtaccaa caccggctca accagcaact ccagtaaatg ctaatccagt
     2821 agcaccaact acaggtaaag aaaaccgtgg ggataaatta cctgaaactg gaagccaatc
     2881 tgattatatc tctgttcttt taggtagcgg tattctattg agcctatatg taggacgaag
     2941 aaaagaagat taatcagcca aaagcagtca ctaatttctc gaaagaagat gataagtgat
     3001 agatctcgaa tgaaagaata ttcttagaat gattttccta tgatggaaaa gactgctaaa
     3061 aaccttgagt ggtaacgctc aaggtttttt tggatggctg atgtttgtct gaaaatcttg
     3121 attgagaaat aaaaagccac cctattaggt ggctataatt gatttagact ttctaaaatt
     3181 ttctattttt tactgaatcg cttcgaccaa gtcttgagct tgtttttcaa gtgagtttag
     3241 gactgtttct tcaagaacca attttccgtc tgcccaggca gagtcattaa cacgtgcagc
     3301 agtgaaatca ccaacgcctt gtgtacggat aaatggcaag aggtctttgt agatagcgaa
     3361 aagttgatcg tgccctgcat tggctacaga tgatactgtg acaaacttgt cttgaagggc
     3421 agaagcgcca cgtgtatcag acaagtcaag ggcacgagat agccagtcaa gcaagttttt
     3481 tactgtacca gggatagaga agttgtagac tggagagaaa atccagatag catccgcaac
     3541 gagaactgct tcacgagcag cagctacagc tggatgtgtt ggaacttcca aatcttggct
     3601 gaagagagga agggctgaat aatcaaggta gctaacttcc gctttaccag caagtgcttt
     3661 ctcagcttcg agcgccattt ggtggttgaa agaaccttga cgtagtgatc cgacgataaa
     3721 taatactttt ttagacatga tgtgtctcct ttttcttcaa atttcgagaa ctctctcgtt
     3781 gttataaaat atattacaac aaaacaaaag aacttgcaag aaatttgctc agagtttatt
     3841 tgaaaattgt aaaatttcgg aaactctagt catttgtgac tgaaaaagta agaactgttt
     3901 cactttcaaa atcggttttg tttgaaggga gaaatagaat tggttgctca aagcacagct
     3961 ttgaggttgc agataaagct gacgtggttt gaagagattt tcgaagagta ttcttatatt
     4021 cacttgtaat aaagcttaat agatggtaaa atagacgagt gaaaaaaaga caaaacaaag
     4081 caaaaaataa tctactgtgg cagtatggtc tagggatgac gattttgttt gtggttatca
     4141 gtgcttcctt tctgtatatg gtttctctta gcatgaaacc ctatcaaaca gctaaaagtg
     4201 aaggagaaaa attagctcag cagtatgcag gattagagca ggccgatcag gttgatttat
     4261 acaatggctt ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa gaagcacttg
     4321 ctgttctgat tggaaaagat gatcataaga tttacgttta tcagctaaat cagggtgttt
     4381 cacaagaaaa agcagaaacg gtttctaagg aaaagggagc tggcgaaatt gacaagatta
     4441 tctttggtcg ttatcaagat aagccaatct gggaagtcaa gtcaggatct gatttttatc
     4501 tagtagattt tgaaacagga gcattggtca acaaggaggg cctatgaaat tatccaaccg
     4561 tgttttagaa atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca aagcactgaa
     4621 ggctgagggc agagatattt tgtctctaac cttgggtgag ccagatttca ctactcccaa
     4681 aaatatccaa gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt tttatacagt
     4741 aacctcaggt ctgccagagc ttaaggcggt ggtcaatagc tactttgagc gcttttacgg
     4801 ctattctgta gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt ctctctatac
     4861 cttctttatg gctgtggtca atccaggtga tgaagtcatc atcccaaccc catactgggt
     4921 tagctatgga gatcaggtca agatggcaga aggcgttccc gtctttgttt ctgctaagga
     4981 agacaatcac tttaaggtga ccgtagagca gttagaagta gctcgcactg acaagaccaa
     5041 ggttttggtg ctgaattcgc catctaatcc cacaggtatg atttacaccc gtgaggaact
     5101 cttggcaatt ggaaactggg ctgtagaaaa tgatattctc atcctagcag acgatatcta
     5161 tggccgcttg gtttataatg gtcatgagtt cacaccgatt tctagcctat cggaagcgat
     5221 tcgcaagcaa acagtggtca tcaatggtgt gtctaaaacc tatgccatga ctggttggcg
     5281 gattggttat gccgttggag aagcagacat tatcgctgcc atgtccaaga ttgcaggtca
     5341 aacaacttcg aatccgtcag cagtagccca atatgcagca gttgaggctc tatcaggtga
     5401 gcaagatact gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata ccatctatcc
     5461 cctccttgca gaggtgccag gatttgaagt ggtcaaaccg caaggggcct tctatctctt
     5521 cccaaatgtc aaaaaggcca tggagatgaa aggctacacg gatgtgacag actttacaac
     5581 tgctatctta gaagaatccg aagtggcctt ggtaacagga gctggatttg gagcaccaga
     5641 aaatgtccgc ctcagctatg cgacagacct agacacgctt aaagaagcag tcgaacgctt
     5701 gaaagcattt atgggtagtg agaatgattg atcattttga gattaaggta aaggatttac
     5761 aaatttcaga aggattttat aggagttttc tcgctccttt ggactataaa ttggctttta
     5821 agactagttc tctaattagt tttctatccc cgaacagccc tcatcctggt ggtgattttt
     5881 ggctgactca gggaacacaa gatcctgttc attttgcttt tttagcagaa aataaggaag
     5941 aagtttaggc ttgttatgag gctggcttag aggcaggtgg gcgagacaat ggggctcctg
     6001 gttatcgaag tgagcgtccg atttactatg ctgcttttat gattgacctg gatgggaaca
     6061 atatagaggt ggtttgccat aaagaataaa aaagaaaagg aaaaataatg acaaaacgtg
     6121 taacgattat tgacgtaaaa gactatgttg gtcaggaagt gacgattggc gcttgggttg
     6181 ccaataaatc aggaaaaggg aaaatcgctt tcttacaatt gcgtgatgga acagccttct
     6241 ttcaaggtgt ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa gtgggacttg
     6301 agaagtttga tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg acaggtattg
     6361 tcaaagagga cgaacgttct aaatttggct atgagttgga catcacagac atcgaagtga
     6421 tcggtgaatc tcaagactac ccaatcacac caaaagaaca cggaacagac tttttgatgg
     6481 ataaccgtca cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa atccgtaacg
     6541 ctattatcta tgcaacttat gagttctttg acaagaacgg ttttatgaag tttgacagcc
     6601 caattctttc aggaaatgcg gcagaagatt ctacagaact ctttgaaact gactacttcg
     6661 gaacgccagc ctacttgagc caatcaggtc aactttacct agaagcaggg gctatggctc
     6721 ttggtcgtgt ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa acacgccgtc
     6781 acttgactga gttctggatg atggatgctg agtactcata cttgacacat gatgagtcgc
     6841 ttgacttgca agaagcttat gtgaaagctc ttctacaagg tgttcttgac cgcgcgcctc
     6901 aagccttgga aaccttggaa cgtgatacag aactcttgaa acgctacatt gcagagccat
     6961 tcaaacgtat cacttacgat caagccattg acctcttgca agagcatgaa aatgatgaag
     7021 atgctgacta cgagcatctt gagcatggtg atgactttgg gtcaccacac gaaacttgga
     7081 tttcaaacca ctttggtgtg ccaacatttg tcatgaacta tccagcagcc atcaaggcct
     7141 tctacatgaa accagttcct ggaaatccag agcgcgtgct ttgtgcagac ttgcttgctc
     7201 cagaaggcta tggagaaatt atcggtgggt ctatgcgtga ggaagattac gatgcccttg
     7261 tcgctaagat ggatgaactt ggcatggatc gtacagaata tgaattctac cttgaccttc
     7321 gtaaatacgg tacagttcca cacggaggat ttggtatcgg tatcgaacgt atggtaacct
     7381 tcgcagcagg aacaaaacat atccgtgaag ctattccatt cccacgtatg ttgcaccgta
     7441 tcaaaccata agaacaggaa aacgcccatg tggcgttttt tcgaattcta gtgtttttac
     7501 ttgcgtaaaa aaattttttc tagtataata gtttattgtg agcgaacctc acttacccct
     7561 tgcaaagtct tggggtcatt agaccaaaag gaggaacata tcaatggcta aatacgaaat
     7621 tctttatatc attcgtccaa acattgaaga agaagctaaa aacgctttgg tagcacgttt
     7681 tgactctatt ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt gggaaaaacg
     7741 tcgtcttgca tacgaaatcc aagatttccg tgaaggactt taccacatcg ttaacgttga
     7801 agcaaatgac gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca acgctgacat
     7861 tcttcgtcac atgatcgtca aaattgacgc gtaagaaggt aaactatgat taacaatgtt
     7921 gtacttgtag ggcgtatgac acgtgacgct gagttgcgtt ataccccatc aaatgtagca
     7981 gttgcgactt ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg tgaacgtgag
     8041 gctgatttta tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc taactgggct
     8101 aaaaaaggct cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta cgataaccag
     8161 caaggacaac gtgtctacgt gacagaggtc gtggctgaga atttccaaat gttggaaagc
     8221 cgtagtgtgc gtgagggtca cacaggtgga gcttactctg caccaactgc aaactattca
     8281 gcacctacaa attcagtacc agacttttca cgtaatgaaa atccatttgg agcaacaaac
     8341 ccattggata tttcagatga tgatttacca ttctaatgga caactaaact tataaaggag
     8401 aaaaaacatg gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag ttgattacat
     8461 cgcagcaaat aaaattgaat atgttgatta caaagatact gagcttctta gtcgtttcgt
     8521 ttcagaacgt gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta aaaaccaacg
     8581 taaagtaaca acagctatca aacgcgctcg cgtaatggct ttgatgcctt tcgtaaacga
     8641 agattaaaga aaagacccgg ttgggtctct ttttaatgag cttgaaaaca agaaagcgaa
     8701 ttaagacccc aacgggtctt ttttcacaag ctttgaaatg agagagcgag ttaggctcag
     8761 tggctccctt tttacgagtt tgaaaacaag agaacacatt acaagcacga tggagctttt
     8821 tcataaatat gtctcttttc tcatccaaga aattaaaata aaaatgataa actttttgaa
     8881 aataggggat ttgcctactt tatgatataa tggaaagagt tagattgaaa gaggtagtga
     8941 gatggacgcg aaattaagat acaaggcaaa gaagatcaag attgtctttt ttgatattga
     9001 tgatacattg cggaattcaa agacagggtt tattccaact acaattccca ctgtttttaa
     9061 acagttgcgt gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca tttttggtgt
     9121 tgttccagag attcgtgatc tcaagcctga cttttttgta actttgaatg gggcttatat
     9181 cgaagataaa aaaggtcagg tcatttatca gcatcagatt ggaaagtcag atgttgagga
     9241 gtatatctct tgggctaagc aagaaggaat tgagtatggc ttggttggga gtcatgatgc
     9301 caagttgtcg actcgcaccg atatgatgag tgaagctatc aatccaattt atcccgactt
     9361 agatgtagat cccgatttcc atgaaaaaga agatatctat cagatgtgga cttttgaaga
     9421 taagggagat gacttgcact tgcctgacag tctctcagac aaacttcgca tggttcgttg
     9481 gcatcaacat tcgtctgata ttgtgcctat ttcaggctcc aaagcgacgg gggtggaaaa
     9541 ggttgtggaa caccttggat tgaaaccaga gaaggtcatg gtttttggag atggtctcaa
     9601 tgacttggaa ctctttgatt atgctggaat cagcgttgcc atgggaattt ctcatgataa
     9661 aatcaaagaa aaagcagatt atattacaaa aacattagaa gaagatggca tttttgatgc
     9721 cttagaagta tttggtatgg tagaaaaaga attgcatttt ccacaagtag acattgaaac
     9781 agtagaaggt cctcttgcga ctattaagac caatcacgga gacttacgta tcaagctttt
     9841 ccctgaacat gctcctaaaa cagtggctaa ctttgtatct ctttcaaaag atggctacta
     9901 tgatggtgtc attttccacc gtattatcaa ggactttatg atccaaggtg gagacccaac
     9961 tggaactggt atgggtggcg agtcaatcta cggcgaatct tttgaggatg aattctcaga
    10021 agagctttac aatatccgtg gtgctctttc catggcaaat gctggcccaa ataccaacgg
    10081 cagccagttc tttatcgtgc aaaaccaaca cctaccttat tctaagaaag aaattactcg
    10141 tggtggttgg ccagaaccga ttgcagaaat ctatgccaat caaggtggga cacctcacct
    10201 agaccgccgt cacacggttt ttggtcagtt agctgatgaa gcatcttacg ctgtcttgga
    10261 tgccattgct gctgttgaga caggagctat ggacaagcca gttgaagatg ttgtaattga
    10321 aactattgaa atcgaggact aagatgaaaa tcggtgataa gctaaagggg cgtattacag
    10381 ggattcagcc ctacggtgcc tttgttgagt tagagacggg tgatacgggg ctgattcaca
    10441 tttcagaaat tcggacagga tttattgaaa atattcatga aaccttgaaa gtcgatgaag
    10501 aggttcaagt tcaggtagtg gatttagatg aatttacggg gaaagccagt ctttctatcc
    10561 gcactttgga ggaagaaaag taccagtttc ctagacggag acgcttttca agcgaccgct
    10621 ttaactacgg ttttgctccc tttcgacgaa tgctaccaat ctggactgga gaagccctgc
    10681 actatttaaa aaagaagaag tagtttagag ttttatattt tttgtaatgt taatataaat
    10741 ttgctataat aggaccatgg aagaagaaca attattaaaa tcaggagagc gcattaacca
    10801 gctcttttcg acagatatca aaatcattca aaatagagag gtttttagct attcggtgga
    10861 tagtgttctc ttatcacgat ttccacgttt tcctaagaag gggttgattg tagatttctg
    10921 tgctgggaat ggagcagtgg ggctttttgc tagcactcgt actcaagcac agatattgtc
    10981 tgttgagatt caggagcgtt tggcggatat ggctgaacgc tctgtccgtt tgaatggatt
    11041 agaggagcaa atgcaggtca tctgcgatga tttgaaaaat atgcctgctc acattcaggg
    11101 gagtaaggtg gatatgattt tgtgtaatcc accctatttc aaggtggatc ctcattctaa
    11161 tctgaacgag agtgaacatt atctcttggc tcgacacgaa atcacgacta atttggaaga
    11221 aatctgtcat agtgcccaga gtattctcaa gtctaatggg cgtttggcca tggttcatcg
    11281 tcctgatcga cttctggata ttttggatac gttaaaacga cataatctag cccctaagcg
    11341 cctgcagttt gtttatccaa aaagagaaaa ggaagccaat atgcttttga ttgaggcgat
    11401 caaggatggc tcaacaagtg gctttaaggt cttacctcct ttgattgtcc acaatgatga
    11461 tggctcttat acgcccgaaa tcgaagagat ttattatgga tcataaggct tatatgtacg
    11521 ttctggagtg tcgtgatgga tcctactata taggctatac gactgatgtg agaagacgcc
    11581 tcgctatcca caatagtggg aagggagcca aatatacacg agcacgcttg ccagtcaaac
    11641 ttatctatgc tcaaggtttt gccagtaagg aagaagccat gtcggctgaa gctcttctca
    11701 agcgtaagaa gaggccacag aaggaagaat ttttatctga aaatcaagat agaaatttac
    11761 tcagttattt tgaagaatcg tggggagtcc tttgactcct cttacctttg tcaaaaagcg
    11821 aaaaaaatgc tacaataaag aaaataaaca aaatgaggta ttatcatgtc caagattcta
    11881 gtatttggtc accaaaatcc agactcagat gccatcggat catctgtagc ttttgcctac
    11941 cttgcaaaag aagcttacgg tttggatacg gaagctgttg cccttggaac tccaaatgaa
    12001 gaaacagcct ttgtcttgaa ctattttggt gtggaagcac cacgtgttat cacttctgcc
    12061 aaagcagagg gggcagagca agttatcttg actgaccaca atgaattcca acaatctgta
    12121 tcagatatcg ctgaagtaga agtttacggt gttgtagacc accaccgtgt ggctaacttt
    12181 gaaactgcaa gcccacttta catgcgtttg gagccagttg gatcagcgtc ttcaatcgtt
    12241 taccgtatgt tcaaagaaca tggtgtagct gtgcctaaag agattgcagg tttgatgctt
    12301 tcaggtttga tttcagatac ccttcttttg aaatcaccaa caacacaccc aacagataaa
    12361 atcattgctc ctgaattggc tgaattggct ggtgtgaact tggaagaata tggtttggca
    12421 atgttgaaag ctggtaccaa cttggctagc aaatctgctg aagaattgat tgacatcgat
    12481 gctaagactt ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa cacagttgac
    12541 atcgctgaag ttttggaacg ccaagcagaa attgaagctg caatgcaagc tgccaacgaa
    12601 tcaaacggct actctgactt tgtcttgatg attacagata tcgtcaactc aaactcagaa
    12661 atcttggctc ttggtgccaa tatggacaag gtcgaagcgg ctttcaactt caaacttgaa
    12721 aacaatcatg ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt acctcaatta
    12781 actgaaagct ttaatgcgta agattttggg tgtcagctca aaatcggaaa gtctagtttg
    12841 ccttatatcg aaaggagttt cggctccttt ttttctagga gtgaagtatg ttagaaaatg
    12901 gcgatttgat ttttgtgaga gatgggtcag acatgggaca ggccatccag acttccacag
    12961 gtaactatag ccatgttgcc atttatttgg atgggatgat ttatcatgct agtggacagg
    13021 ctggtgttgt ctgtcaagaa ccggcagact tctttgagtc caatcattta tacgacctct
    13081 atgtttaccc agaaatggat atccagtcgg tgaaggaaag agcttgcaga catcttggag
    13141 caccctacaa tgcttctttc tatccagatg cagctgcttt ctactgctcc cagtatatag
    13201 cagaaatcct acctattttt gaaactattc ctatgaaatt tggagatggg gaacaggaga
    13261 ttagtgattt ttggagggag tattacatag aactaggtct gcctgttcct ctgaaccaag
    13321 ctgggaccaa tcctagtcag ttggcagcat cgcctctgtt acaatgtaaa gaaagggatc
    13381 ttcatgattc agatttttaa tccatctcgt ttgacgagac agccattttt ggagaattga
    13441 tccgctatct ggaccagtat gaggatgtga ttctacggga aattaagtct cagtttccag
    13501 atgttgcagt tgataaactc atggaagagt atataaaggc aagcttgatt ctacgtgaaa
    13561 ataagcgcta ttaccttaat tttcctacgc ttgaatcact tgatagtctt gaactggatc
    13621 aagagatttt tgtcagagaa gctagtccgg tctatcaagc cttgttggag cagagttttg
    13681 agacggaatt gcgcaatcaa atcaatgcag ctattttagt tgaaaagacg gactttgcgc
    13741 gcattaaaat gaccctgtcc aattattttt acaaggtcaa acagcagtat cctttgacag
    13801 aaaaacagca ggagctctat gacattttag gagatgttaa tcctgagtat gccctcaagt
    13861 atatgacggc ttttttgttg aaatttctca aaaaagacca gcttatgcag aaatgccgtg
    13921 atatctttgt ggacagtttg gttgtcttag gctatattgt gcaaaatgaa gatagaaagt
    13981 atgagttggc tatcgatttt gataaggaga gattaacttt ctacttagcg tgatttcttg
    14041 tttctgagca cattgtttga ctttccttag tattcggtat aaactatatg taaccggtaa
    14101 cacatatcgg aataaactaa aggagacaat catatgtcac ttgaaaacaa attggaacaa
    14161 gcaacaggcg ctgtcaaaga aggttttggt aaagttactg gagacagcaa gacagaactt
    14221 gaaggagctg ttgaaaaaac agttgctaag gcaaaagacg ttgtagaaga cgcaaaaggt
    14281 gctgtagaag gtgccgttga aggtttgaaa aacgttttta ctaaataata ggaaaaaatc
    14341 aagggtttca ttttcccttg attttttcta ttcttataaa taattttctg cgacggctgt
    14401 atctcctggg taggattctt tcttgccctg gatgatttgg taacaatcgg ctcccttacc
    14461 cgcaataata actgcatcta attcgtgatt tgtgatagcc attgccgcct tgatggcttc
    14521 ttggcgatcc gcaatctttt caacaggatg attgatgtag ctactaattt catctgcaat
    14581 ggccattggg tcttcatagt tagggtcatc agcagtcaga aagacttgaa tctcagggtg
    14641 ttgattgagg aggaggccaa agtccttacg acgactttct cccttgtttc ctgttgatcc
    14701 cagaaccaga gcaatctttc cggtttgatg agtttcaacc acattgatga gttttttcag
    14761 actatcccca ttgtgggcat agtcgatgaa gaccttggct ccatttttct gagtgaggac
    14821 ttccatacga ccaggaacgc gggttgcagc gatgcctttt ttgatgtcct caagacttgc
    14881 tccgagacgg agacaagcaa gtccagcagc aactgcattt tcttggttga agttgccaat
    14941 gagttggata tcataatctc cagcgagttt acccgtagct gaaaagctaa aggctttgga
    15001 attctcgatt tggttatcaa attggctacc atagaaatca tggtcttgat cttcaacctg
    15061 ttctttcaag actgagaagt ggtccatgtc actgttaatg atgactgctc ggctattttc
    15121 catcaagaga cgcttgtggt agaaatagtc ttcaaagcta gggtgttcaa tcgggccgat
    15181 atggtcagga gtgatgttaa gaaagactcc tacatcaaag gtcagtccat agactcgatg
    15241 gactagatag gcttggctag agacttccat tacgagatgg cttcttccat tcttaacagc
    15301 ttgagccatc atgtcaaaga ggtcgatatt ttcaggtgtt gagaatgaag atttaaagaa
    15361 agttgtgcca tccaaagttg tattcatggt tgataagagg gcggttgggt aacgttgaga
    15421 taggatgttg taagcaaagt aagctgctgt tgtcttaccc ttagttcctg tgaaggcgag
    15481 aattttgagt ttctcttgtg gattaccata gaactccatg gcaatcaaac tcatggcttt
    15541 ctttatatcg ttcacaatga tgacagggat accgacttcg tagtcctttt cagctacata
    15601 ccaagctaaa ccttgtgtta tagcagaaag aaggtattct tttttaaagg cagcaccttt
    15661 tgcaaaaaaa agagtgtctt ctgttacttt tcggctgtcg tagctgatgc tatcaaaaac
    15721 aactttgctg tagttgtagt ggtaatgacc ttggtcaata atttcgcgaa aaaggccatc
    15781 tttctttaaa atatctaata cagtttcaat ttttatcata ctttctattg taaaccgaaa
    15841 gtcgcaaatt tacaagtaac aaggaaaagt ttataatgga agataaggag tttttcctag
    15901 ttatcaaaat tgaatgagga atctatgtcg cacgaaaaca atcaccagca ggcccagatg
    15961 ttacggggga ctgcttggct aacggctagt aactttatca gtcgcctact cggggctgtt
    16021 tacattatcc cttggtacat ctggatgggg gcttatgcag ctaaggcaaa tggtctcttt
    16081 accatgggtt acaatatcta tgcttggttc ttgttggttt caacagcggg gattccagtt
    16141 gcggtggcca agcaagttgc caagtataat accatgcgag aagaagagca tagctttgcc
    16201 ctgattcgga gcttcttagg ctttatgaca ggactaggcc tggtttttgc tttagtcttg
    16261 tatgtctttg ctccttggct agcagacttg tctggcgtgg gcaaagactt gatcccaatc
    16321 atgcaaagct tggcttgggg agtcttgatt ttcccgtcta tgagtgttat ccgaggattt
    16381 ttccaaggga tgaataacct caaaccttat gccatgagcc aaattgctga gcaggtcatt
    16441 cgtgttatct ggatgctcct agcaaccttt atcattatga agctcggttc aggagattat
    16501 ctagcagccg ttacccaatc aacctttgct gcctttgtcg gtatggtagc cagttttgca
    16561 gtcttgattt atttccttgc ccaagaaggt tcactcaaaa gagtctttga aacaggagat
    16621 aagattaaca gtaagcgtct cttggttgat accattaagg aagccattcc ttttatcctg
    16681 acagggtctg ccatccagct cttccagatt ttggatcagc tgacctttat caatagtatg
    16741 agctggttta ccaactacag caatgaggac ttggttgtca tgttttctta tttctcagcc
    16801 aatcctaata aaatcacgat gattttgatt tctgtagggg tttcgattgg gagtgttggt
    16861 ttgccacttt tgacggaaaa ctatgtcaag ggggacttga aagcggcttc tcgtctcgtt
    16921 caggacagtc tcaccctact ctttatgttc ttgctaccag caacggttgg agtggttatg
    16981 gtaggagaac ctctttatac ggtcttctat ggtaagccag atagtttggc tctgggctta
    17041 tttgtctttg cagttttgca gtctattatt ttaggcttgt acatggtctt gtctccaatg
    17101 cttcaggcca tgttccgcaa ccgcaaggcc gttctctatt ttatctatgg ttctattgcc
    17161 aagctagtct tgcaactacc taccatcgcc ctcttccaca gttatggtcc tttgatttca
    17221 acaaccattg ctctcatcat tcctaacgtc ttgatgtatc gggatatttg taaagtaact
    17281 ggtgtcaagc gcaaggtgat tttgaagcga accattttaa tcagtttgct gaccctagtc
    17341 atgtttctgt taataggaac catccagtgg ctgttaggat ttttcttcca accaagtgga
    17401 cgtttgtgga gcttctttta tgtagctctt gtcggtgcca tggggggtgg actttatatg
    17461 gttatgagtc tgcgtaccta tttattagat aaggtaatag gaaaagccca agcagatcgc
    17521 ctgcgagcaa aatttaagct ttcgtaaggt agcatttata aataaaagga ataagttgaa
    17581 caatctttta ggagaacttg aaacttattc cttttttagc gtcaaaatag ctaaaattat
    17641 ctttcaaaaa tagaaaatga gttttttgat tttttggaaa aaacaattga ctaaacaaac
    17701 caagagttgt ataataaaac aaatatttaa agcaggaggt tctggaaatg aaaaagtcta
    17761 agagcaaata tctaaccttg gcaggttttg tcctgggtac aggagtttta ttgagcgcgt
    17821 gtggaaattc tagcactgca tcaaaaacct ataactatgt ttattcaagt gatccatcta
    17881 gtttgaacta tctagcagaa aaccgcgcaa caaccaatga tattgtgacc aatctggtag
    17941 atggtctcat ggaaaatgac caatatggga actatgtgcc atccttggca gaagattgga
    18001 ctgtttctca ggatggtttg acctatacct acaaacttcg taaggatgcc aagtggttta
    18061 cttctgaggg agaagaatat gcacctgtaa ctgcccagga ttttgtgaca ggattgcaat
    18121 atgcagctga taaaaaatca gaagccttgt atctagtgca ggactctgtt gcaggtttgg
    18181 atgactatat cactggtaaa acaagcgact tttcaactgt cggtatcaag gctcttgatg
    18241 atcaaaccgt tcaatacacc ttggctcgac cagaacctta ctggaactca aaaacaacct
    18301 caaccattct tttcccagtc aatgcagatt tcttgaaatc aaaaggggat gattttggca
    18361 aggtagaccc tgctaatatt ttgtacagtg gacctttctt gatgaaagct tttgtttcaa
    18421 aatctgtcat cgaatacaag aaaaatccta actactggga tgctaaaaat gtctttgtag
    18481 acgatgtgaa attgacctac tatgatggta gtgaccaaga tgctcttgtt cgtaacttta
    18541 cagacggagc ctatagctat gcccgtctct atccgaatag ttcaagtttt gaagggatta
    18601 aagaaaaata caaagatgat atcatctaca gtatgcagga tgctacgtct tattactgga
    18661 attttaacct agatagacaa gcttataaat ttacttctaa gacgaccgac attgaaaaga
    18721 aatcaactca ggaagcagtt cttaataaaa acttccgtca agctattaac tttgcctatg
    18781 atcgtacctc ctatggggct caaactcagg gagaagacgg agcgactaag attttgcgta
    18841 acttggttgt tcctccaacc tttgtaagta tcaaaggaaa agattttggt gaagtagtag
    18901 cctctaagat ggtcaataat tatggtaagg aatggcaagg catcaacttt gcggatggtc
    18961 aagaccctta ctacaatcct gagaaagcca aggctaaatt tgctgaggcc aagaaagagc
    19021 tagaagctaa gggagttcaa ttccctattc atttggatgc gacagttgac caagcttcta
    19081 agaaggggat tcaagaagta agctccatga agcaatctat tgaagcagct ttaggtacgg
    19141 agaacgtcgt tattgatatc caacagttat caacagagga ctatgataac tcaagttacc
    19201 ttgctcagac tgctattcag aaagactacg acctttacaa tggtggttgg agtcctgact
    19261 acttagatcc atcaacctat cttgatatct ttagtgtcaa gaatggtggg gttctacaaa
    19321 atcttggact cgaccctggt gaagccaatg acaaggctaa ggcagttgga cttgacactt
    19381 atactcaaat gttggaagag gctaataagg agcaagaccc agccaaacgt tatgagaaat
    19441 atgctgatat tcaagcatgg ttgatagata gttctctagc gattccaaat gtttcattag
    19501 gtggaacgcc tagcttgaga aaaacagttc ctttctcagc accttattca ttggctggta
    19561 ataaaggggt tgaatcatat aaatacctca aagtacaaga taagattgtc acaacagacg
    19621 aatatgcaaa agccagagaa aaatggttga aagaaaaaga agaatccaat aaaaaagccc
    19681 aagaagaatt ggcaaaacat gtcaaataaa atctaattgt agataagttt gtgtaagaag
    19741 tctttgtgct taaaaacgca taatatcagg tatataaaat cctagatgtt atgcgttttg
    19801 ttataggaag atttattaga ttttatcctc aaattgagtt attcccgact ttatccgttg
    19861 aaatattata aggagtattt atgaaattaa aattattaag agtagatact aaggtgatta
    19921 tgtggagttt cttacttgtt ctgtctagtc tacttgcttt gttgcttccc cttatcttaa
    19981 agggtttaat agatgggagt tctattgaaa atataggttc caaagtattt caatcgtttt
    20041 tgatttttat tggtcaagcc ttgttttctt ctattggtta ctatctgttt agtcaatcgg
    20101 gtgaaaaaag atagcaaaaa tcaggaaaaa agtgatagag gggttgattt atgcagagaa
    20161 atccttcttt gataagagcc aaagtgggga gttgacttct gccattgtca atgacatgag
    20221 tgtcattcgt gagtttttaa ttacgacttt cccaaatatt attctgagtt tagttatggt
    20281 acttggttcc attgtagtct tatttagtct tgattggaat ctttctctac ttttattcat
    20341 cactcttcct tgtatgatgt ttattatctt gcccctttcc aatatcagtg aaaagtatag
    20401 tcgtcgttta caggaggaaa tcggattttt aacagggcaa ttgactgaaa agattcaaga
    20461 acatgaatta attaagacta accaagcaga gaaaagtgta caggatgttt tggataattg
    20521 tattgaaagg gtacaaaaca attcactgaa atcagatagg gttacttctt ttgagacccc
    20581 gtttgctctc ttatttatct ttgcgactat agttgtgatg ctaacctatg ggggttatcg
    20641 ggtcagcgca ggatatatat ctgtgggaac cttggtttcg tttttgattt acctctttca
    20701 attacttaat cctattagta atatagctaa ttttgtaact atttattcta gtagcaaggg
    20761 atcttcagtt gcactggaga acttgcttgc agttcctaaa gaaaaatttg agggaggaaa
    20821 atcggtatca ggacaagggt tgaattttaa ccatgtctat tttggttatg atgaaaatcg
    20881 acctgtctta aaggatatta cttgttcaat tttcaagggg caaaaaattg cttttgttgg
    20941 accatctgga tcaggaaaat caacgattgt gcgtttgtta gagcggtttt ataaaccgct
    21001 ttcaggagat attctaatgg agcaatcaag tatatatgat tttaacttaa aagaatggag
    21061 aagtaaaatc gcttgggttt cacaaaataa tgcagtctta tctggcagta ttcgtgacaa
    21121 tctttgtctc ggtttgaatc gcttagtaac tgatgatgaa ttgatgaaag tgctagactt
    21181 agtatcacta ggtgatgaga ttcgctccat gaaagaggga ctagatactg aagttggtga
    21241 acgcggacga ttcttgtcag gggggcaaag ccaaagactt caaatagcta gaacctactt
    21301 aaaagatgct gaaattctta tatttgatga agccactgct aatcttgatg cggattctga
    21361 gtatgcgatt atcagtagcc tctattctgt attaaaggag aagacggttg tgattatagc
    21421 gcatagtttg tcaacggtaa aagatgtgga ttgtattttc ttcttagagg aggggaaaat
    21481 cactggctca ggaactcata aggaactact ggaaaatcat gagcgttatg ctcgttttgt
    21541 gcaggagcaa atgatagagt gaaggtgtct tttgagattc accattttat agtctattaa
    21601 agggagcagg aaaaactccc tttttatata gtttgaaact ataactagct cttgaaaaga
    21661 agaaaatgag ttgatgaaaa taagtggtac aatagttact atagatttgg aggtattgta
    21721 tgagcaagga attacacatt aacacaattt tggcccaggc gggtattaag tcagatgagg
    21781 caacaggtgc tttggtgaca ccacttcatt tttcaacgac ctatcagcat ccagagtttg
    21841 gtcgatctac tgggtttgac tatacgcgca ctaaaaatcc aactcgtagt aaggctgagg
    21901 aagtcttggc ggctattgag tcagcagact atgccctagc gactagctca gggatgtcag
    21961 ctattgtact ggcctttagc gtctttccag taggaagtaa ggtcttggca gtgcgtgatc
    22021 tttacggtgg ttcttttcgc tggtttaacc aagtggagca ggaaggtcat ttccatttta
    22081 cctatgccaa tacagaagaa gagttgattg ccgagttaga aaaggatgtg gatgttctct
    22141 atatcgaaac cccaaccaat cccttgatgt tggaatttga tatcgaaaaa ctagcaaaat
    22201 tggctcatgc taagggtgcc aaagtggtgg tggacaatac cttctatagc cctatctacc
    22261 aacgtccgat tgaagatgga gcagatatcg ttctccattc agcaaccaag tatctagcag
    22321 gccacaatga tgtcttggct ggagtggttg tgaccaatag tttagaacta tacgagaagc
    22381 ttttttacaa tctcaataca acaggggcag tcttgtctcc atttgacagc taccagttgc
    22441 ttcgtggtct caagacctta tctcttcgta tggagcgctc aacagctaac gcccaagaag
    22501 tggttgcctt tttgaaggat tctccagcag ttaaggaagt tctctacact ggtcgtggag
    22561 gcatgatttc ctttaaagta gccgatgaaa cacgcattcc tcatattttg aacagtctca
    22621 aggtcttctc ttttgcggaa agtttgggcg gagtggaaag tcttattact tatccaacga
    22681 ctcaaactca tgctgatatt ccagcagaag tacgccattc ttatggtttg acagatgacc
    22741 tcttgcgttt gtctattggg attgaggatg ctagagattt gattgcagat ttgcgccaag
    22801 ccttagaagg ataagacaaa gatgggaaaa tatgatttta caagcctgcc caaccgttta
    22861 gggcaccata cctataaatg gaaagaaaca gaaacggata gtgaagttct accagcttgg
    22921 atagcggata tggactttgt ggtcttgcct gaaatccgcc aagccgtgca aacttacgca
    22981 gaccaactgg tttatggtta tacctatgcc agtgaagact taattaagga agttcaaaag
    23041 tgggaagcta cacaatacgg ttacaacttt gacaaagagg ctcttgtctt tatcgagggt
    23101 gtggtaccag ccatctcaac agctattcaa acctttacaa aagaaggcga ggcggtttta
    23161 attaacacgc ctgtctaccc accctttgct cgcagtgtca agttgaataa tcgtagattg
    23221 attactaatt ccttagtgga aaaggatagt ctgtttgaga ttgactttga ccaacttgaa
    23281 aaggatttgg tggaagagga ggttaaactc tatgttcttt gcaaccctca caatcctggt
    23341 ggacgtgttt gggaaaaaga agtgttggag aagattggcc aactctgcca aaaacacggt
    23401 gttttgttag tttcggatga gattcaccaa gatttgaccc tctttggtca caaacaccag
    23461 tctttcaata ccatcaatcc tgccttcaaa aattttgcta tcgtcttgag cagtgccact
    23521 aaaacattta atattgctgg aacaaaaaat tcctatgcag tcattgaaaa tcctaagttg
    23581 agactggctt tccagaaacg cctgttggcc aataatcagc atgaaatttc aggcttgggt
    23641 tatttggcga cagaagctgc ctatcgatac ggtaaagatt ggctagagga actcaagcaa
    23701 gtctttgaag accacatcaa ttatgtgatg gatctatttg gaaaagagac taaaatcaag
    23761 gtcatgaaac cgcaaggtac ctacttgatt tggcttgact tttcagctta tgacctgact
    23821 gatgaaacat tgcaagagtt gttgagaaat gaagccaagg ttatcctcaa ctgtggtttg
    23881 gattttggag aggaaggaag tctccatgcc cgcatcaata tagctatgcc caaatctctg
    23941 ttgcaagaag tctgtcagcg gattgtggct acttttgcca aacgttaaaa atccagcctt
    24001 ctaggagaaa agtcttccta gaaggctatt ttcataggcg aaaatatggt ataataaaaa
    24061 gataaggtaa aggtgaaaat atggctaaat tgattccggg gaaagttcgt atcgaaggtg
    24121 ttgcccttta tgaaactggt aaggttgata tcatcaagga aaagaacaat cggctctacg
    24181 ctcgcgttgc agaagaagaa ctgcgctata gtttagagga tgatttggtt ttttgtgcct
    24241 gtgatttttt tcaaaagagg ggttactgtg tgcatttggc agcgctagag cattttctga
    24301 aaaatgatga gcgtggtcag gaaatcttgt ggagtctgga agaaggtcat gaagaaaaag
    24361 aggccgttga aaccaaggtg accttgggtg gcaagttttt ggatcgaatt ttatctccaa
    24421 aatcagaatg cgcctatgag ttatcagctg tgggtcaggt ggaagcagga accaatcata
    24481 tcttgtggac tctgagaatc ggccaaatca atagccaaaa atactatgtc attcgggata
    24541 ttccactttt tttaaggatt gtcgagcaga gaaagtcgta tatgattggc aagatttatg
    24601 aggagtctct ttcttgggaa gcctttgatg atgccagcca agaacttttg actttcttgc
    24661 acggactaac ggaggaaggg ctggtttcag acctcttttt ccaaaaccaa ggtcggcacc
    24721 tctttttccc tctgaccttt tttgagcagg gtgtgaattt actgatggcc ttgccccatt
    24781 ttcaatttga ccatcaagtg gatagctacc aaaccctgct ttttcaggat atgcatgctg
    24841 atgccaatct atttgccttt acagtaacag aatactcgga ctattttgaa atggaaatca
    24901 gtgagagtcc tagggtcaat gtcttttatc agggagcagt acttttccat aaaggtcagg
    24961 tttatttttt aacagaccag cagatgcgtc ttctcaagga aattaaagcg ttacctgtgg
    25021 gtcaacaagg aaagaaatac ctgcaatttg atagcagtga tcgagacaaa ttggcctcct
    25081 gtctgactct ttttatccag atgggcaccg tttcagctcc agaacgtcta caaatcaaaa
    25141 cttttgcgcc ctctttttac tttgacaggg aagaagataa tcggattcgt ttggaaattc
    25201 agtttgatta tggaaataga caggtatcta gccgacaaga gctagaagaa ttaccatttt
    25261 caagtgacgc agacttagaa gaaagaattt tccaagtctg tttggctgcg ggctttgaag
    25321 ccgattttca atcttggcgt caggccttaa aagcggagtc tgtctatcat ttttttcatg
    25381 atatcattcc agtctttgaa aaactcgggc atgttgacct atcagataaa ctagaagagc
    25441 tttatagcct agcgagtcct caggtgcaga ttgcctccaa gggaggtctc ttggaaatcc
    25501 agtttgattt tcaagatatt gcccaggagg aaatcgacca agccatgcag gccttggttg
    25561 ccaatcagga tttttatatt gattcgtcta atcaagttta ctttttcgat gaagaaacca
    25621 agaaaattcg ccaaaatcta caggaactgg ggcaatttga attaaaagat gggaccttgc
    25681 aggctcggaa atccttggcc tacagtttag ctcatctctt tgaagggcga gaccgtgttt
    25741 ctttttcaca agaattccag aatctggccc aggatttgac acatccagag gactttcctc
    25801 gacaggcaac tcaggttcag gctgacttgc gagattatca ggaaaaggga attggctggt
    25861 tgcagatgct ccatcattat ggttttggtg ggattttggc tgatgatatg ggacttggga
    25921 aaacccttca gaccattgct tttttgacta gtcaagtgac aaaagaaagt cgggttttga
    25981 ttctcgctcc gtcaggtttg atttacaact gggcagatga gtttcagaaa tttgccccac
    26041 agttggatgt ggctgttgtt catggtttga aagcaagtcg tgaggagatt cttgccgaga
    26101 gccatcaaat ctatgtgact agctatgcca ccttccgtca ggacagtgag ttttatcagg
    26161 ggatggcctt tgacttcctt ttcttagatg aggctcaggt catgaaaaat gcccagacca
    26221 agattgccca gaccttgaga caatttgtgg tgccgtcggt ctttgccttg tcaggaactc
    26281 cgattgaaaa ccatctgggt gagttgtggt ctattttcca aatcgtcatg cccggacttt
    26341 tgccaagcaa gaaagaattt atgaaattgc cagcagagcg agtggctcag tttatcaagc
    26401 ctttcgtgat gcgacgcaag aaagaagaag ttctgactga attgccagac ttgattgagg
    26461 tggtttataa aaatgaactg gaagaccagc aaaaggctat ttaccttgcc cagttacaac
    26521 agatgcgaga ccatctggct caagtgtcag aacaggaatt tcagcgaagt cgtgtggaaa
    26581 ttttatctgg tttgatgcgc ttgcgtcaaa tatgtgacac tcctgccctg tttatggaag
    26641 attatcaggg agccagcggc aaactagata gtctccgaga cctactggta caggtggcag
    26701 acggcggaca ccgtgtcttg attttctctc agttcaaggg aatgttggaa aaaattgaac
    26761 aagaactgcc agacttgggc ctgacatcct ttaaaattac gggttcaacc ccagccaagg
    26821 aaagacaaga catgaccaag gcctttaacc aaggagaaag agatgccttt ctgatttccc
    26881 tcaaggctgg tggggtcggt ctgaacctga caggtgcaga tacagtgatc ttggttgacc
    26941 tttggtggaa tcctgcggtg gaagcgcaag ccattggccg tgcccatcgg atgggtcagg
    27001 aagaaacggt tgaggtctat cgcttggtga ccaagggaac cattgaagaa aaaattcagg
    27061 aactccaaga acaaaagaaa catctggtgt cacaagtatt ggatggcaca gagtcacgtg
    27121 gtagtctgac cctagcagaa attagagaaa ttttgggaat ttctgaagcc agcacttgaa
    27181 aaatcaagac aatacgctat aatgaagtgt tgaaaggaaa tagaaaatga aacaagaacg
    27241 atttccattg gtgtcagatg acgaggtcat gttgactgaa atgccagtca tgaatctcta
    27301 tgatgagtct gatctgatca gtaatatcaa gggtgagtat cgagataaaa attatttaga
    27361 atgggctcct attactgaag aaaaaccagt aaaaccgatt gaaaagcaag tcgaaaaacc
    27421 taaaaaggct cctttagggg ttaaaaaaga aggaaagagc tatgcggagg tggcgcgtga
    27481 agaagcgcgt gcggacttga aaaagaaacg ctctgctaac tacctaactc aggatttcag
    27541 ccttgcgaga cgtcattctc agcccagtct agttagacag ggcaatcaac cgacaactcc
    27601 tttccaaaag gaaaatcctg gtgaatttgt caaatatagc caaaaattga cccagtctca
    27661 ttatatcttg gcggaagaag ttcattctat ccctaccaag aatgaagaag tgtcagcacc
    27721 tgctccaaag aaaaacaatt atgattttct aaagaagagc caaatctaca ataaaaaaag
    27781 taaacaaaca gaacaagaac gtcgggttgc ccaagagttg aatctgacca gaatgacaga
    27841 ataggggaga aaacatgtca aagacatatc attttatcgg aattaaggga tcagggatga
    27901 gtgccttggc cttgatgttg caccagatgg ggcacaaggt tcagggatca gatgttgaaa
    27961 agtactactt tacccaacgc ggtcttgagc aggcaggaat taccattctt ccttttgatg
    28021 aaaagaatct agacggtgat atggaaatta tcgctggaaa tgcctttcgt ccagataaca
    28081 acgtcgaaat tgcctatgcg gaccaaaatg gtatcagcta caaacgttac catgagtttc
    28141 taggtagctt tatgcgtgac tttgttagca tgggagtagc aggagcacat ggaaaaactt
    28201 caacgacagg tatgttgtct catgtcttgt ctcacattac agataccagc ttcttgattg
    28261 gagatgggac aggtcgtggt tcggctaatg ccaaatattt tgtctttgaa tctgacgaat
    28321 atgagcgtca cttcatgcct taccacccag aatactctat tatcaccaac attgactttg
    28381 accatccaga ttatttcaca agtctcgagg atgttttcaa tgcctttaac gactatgcca
    28441 aacaaattac caagggtctt tttgtctatg gtgaagatgc tgaattgcgt aagattacgt
    28501 ctgatgcacc aatttattat tatggttttg aagctgaagg caatgacttt gtagctagtg
    28561 atcttcttcg ttcaacaact ggttcaacct tcaccgttca tttccgtgga caaaacttgg
    28621 ggcaattcca cattccaacc tttggtcgtc acaatatcat gaatgcgaca gccgttattg
    28681 gtcttcttta cacagcagga tttgatttga acttggtgcg tgagcacttg aaaacatttg
    28741 ccggtgttaa acgtcgtttc actgagaaaa ttgtcaatga tacagtgatt attgatgact
    28801 ttgcccatca tccaacagaa attattgcga ccttggatgc ggctcgtcag aaatacccaa
    28861 gcaaggaaat tgtagcagtc tttcaaccgc atacctttac aagaaccatt gccctgttgg
    28921 acgactttgc ccatgcttta aaccaagcag atgctgttta tctagcgcaa atttatggct
    28981 cggctcgtga agtagatcat ggtgacgtta aggtagaaga cctagccaat aaaatcaaca
    29041 aaaaacacca agtgattact gttgaaaatg tttctccact cctagaccat gacaatgctg
    29101 tttacgtctt tatgggagca ggagacatcc aaacctatga atactcattt gagcgtctct
    29161 tgtctaactg gacaagcaat gttcaatagg atgttcccat ggaaattcca attaagatca
    29221 ttcaggcaag caagtttgat ttgcctgaga taggggcact tcagacctcg tcttttccag
    29281 ctgaaaagca gcaactttcc catattttag aagaaagtat ccgtaagtgt gcggatacct
    29341 ttcttctagc tagggatgaa aatcaacttt taggctatat tttatcaagt ccccagtcag
    29401 acaatccgca atgtctaaaa gtacattctt tagtcatcga gtctgaccat cagagacagg
    29461 gcctgggaac acttcttctt gcagccttga aagaggtggc agttgagctg gattacaaag
    29521 ggattcgttt ggagagtcct gatgagctgc tttcctattt tgaaatgaac ggttttgttg
    29581 atgaagaaga aactttgctc tatgcaacta gccagggcta tagtatgatt tggtttaatc
    29641 ccttttatct ggaggaacaa tgaaaatcag acaagcaaga ttagaagatt tggatcggat
    29701 tgttgaactt gaatttgaaa atttttcggt cgaagaagcc attcctcctt ctgtctttga
    29761 agcacatttg agagaaattc agacctcttt tctggttgct gaaaaagaag gaagaatcat
    29821 gggttatatc gaaggaccag ttggcctgca ccgccatctg caagaccagt cttttacaga
    29881 agaaataaaa gactatagtc atgagcctgg tggttatatc tctgtgacct gtctgtctat
    29941 tgccaaggag gcacagggac tcggactggg tcagaaattg ctgacagcct tgaaagaagt
    30001 tgctcttgaa gatgagagag atggcattaa tctaacctgt catgactatc tcatcgccta
    30061 ttatgaaaaa catggatttg tcaacgaagg ccagtcccag tcaacctttg caggggaaac
    30121 atggtatgat atggtctggg aaatgaaaaa ataagttagg aaaagtatca tagattgctt
    30181 tttttggact tttaagatat aatattatgg attatcaaca aggaggaaaa acttttgagt
    30241 gaaaagtcaa gagaagaaga gaaattaagc tttaaagagc agattctgag agatttagaa
    30301 aaagtaaaag gctatgatga agttctgaaa gaagatgagg cagtagttcg cactcctgca
    30361 aatgaacctt cagctgaaga actcatggct gattccttgt caacggtaga ggagattatg
    30421 agaaaagctc ctaccgtgcc tactcaccca agtcaaggtg taccagcttc tccagcagat
    30481 gagagtcaaa gagaaactcc tggtgttcca agtcatccaa gtcaagatgt accttcttct
    30541 ccagcggaag aaagtggatc aagaccaggt ccaggtcctg ttagacctaa gaaacttgaa
    30601 agagaataca atgaaacccc aacaagggta gctgtttcct atacgacggc agagaaaaaa
    30661 gcagaacaag caggtccaga aacacctacg cctgctacag aaatagtgga tatcatcagt
    30721 gatacatcac gtcgtagccg tagagaagga gcaaaacccg ttaagcctaa gaaagagaag
    30781 aagtcacatg tgaaagcttt tgtgatttca ttccttgtat tccttgcctt gctctcagca
    30841 ggtggttact tcggttacca gtacgtgcta gattctttgt tacctatcga tgctaattct
    30901 aagaaatatg tgacggttgg aattccagaa ggttcaaacg ttcaagaaat cggtacgacg
    30961 cttgaaaaag ctggtttggt aaagcatggt ctgattttta gtttttatgc caagtataaa
    31021 aattataccg acttgaaagc aggttactac aatttgcaaa agagtatgag tacagaagac
    31081 ttactcaaag agttgcaaaa aggtggaaca gatgaaccgc aagaacctgt acttgcgact
    31141 ttgacaattc cagaaggtta taccttggat cagattgctc aagctgtggg tcaattgcaa
    31201 ggtgacttca aagagtcttt gacagcggag gctttcttgg ctaaagttca agatgagacg
    31261 tttatcagtc aagcagtagc gaaatatcct actttactgg aaagtttgcc tgtaaaagac
    31321 agcggtgcgc gttatcgttt ggaaggatac cttttcccag ctacatactc tatcaaggaa
    31381 agcacaacta ttgagagctt gattgatgag atgttagctg ctatggataa gaacctatct
    31441 ccttactata gtactatcaa atctaaaaat ttgactgtca atgagttgtt gaccattgct
    31501 tccttggtcg aaaaagaagg tgccaagaca gaagatcgta agctcattgc aggtgtattc
    31561 tacaatcgtt taaatcgtga tatgccactt caaagtaata ttgcaatctt gtatgcccaa
    31621 ggaaaactgg ggcaaaatat cagtctagct gaggatgttg cgattgatac caacattgat
    31681 tcaccttata atgtttataa aaatgtaggt ctcatgcctg gtccagtcga tagtccaagt
    31741 ctggatgcga ttgagtcaag catcaatcaa actaagagcg ataacctcta ctttgtagca
    31801 gatgtcacag aaggcaaggt ctactatgct aacaatcaag aagaccacga ccgcaatgtc
    31861 gctgaacatg tcaacagcaa attaaactaa acaaactaaa attatgtgat acgtcacata
    31921 attttcttta gaaaatatta tcagaagaaa gttgagaaaa atggcagaaa aaacatatcc
    31981 tatgaccctt gaggaaaagg aaaaacttga aaaagaatta gaagaattga aattggttcg
    32041 tcgaccagaa gtggtagaac gcattaagat tgcccgttca tacggtgacc tttcagaaaa
    32101 cagtgagtac gaagcagcta aggatgaaca agcctttgtc gaaggacaaa tctctagctt
    32161 agaaacaaaa atccgctatg ctgaaatcgt caatagcgac gcagttgccc aggacgaagt
    32221 agcgattggt aaaacagtca ccatccaaga aattggtgag gacgaagaag aagtttatat
    32281 tatcgtaggt tcagctggtg cggatgcctt tgcaggtaag gtttcaaatg aaagcccaat
    32341 tgggcaggcc ttgattggca agaaaacagg tgacacagca accattgaaa cgcctgttgg
    32401 tagctatgat gtaaaaatct tgaaggttga aaaaacagcc taaaaacaga aaaaggagtg
    32461 gggaggcgat gtgcttcact cactcctttt tccattttgc tattcttcga aaatctcttc
    32521 aaaccacgtc agcgtcgcct tgccgtatgt atggttactg actttgtcag tttcatctac
    32581 aacctcaaaa ccatgttttg agctgacttc gtcagtttca tctacaacct caaaaccatg
    32641 ttttgagctg acttcg
//
LOCUS       DAVHIT010000016        32586 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVHIT010000016 DAVHIT010000000
VERSION     DAVHIT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.016"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            128..676
                     /locus_tag="VVN16_000601"
     CDS             128..676
                     /locus_tag="VVN16_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5572044.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            786..1685
                     /locus_tag="VVN16_000602"
     CDS             786..1685
                     /locus_tag="VVN16_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU5572045.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1710..2984)
                     /gene="serS"
                     /locus_tag="VVN16_000603"
     CDS             complement(1710..2984)
                     /gene="serS"
                     /locus_tag="VVN16_000603"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU5572046.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKKIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3198..3569)
                     /locus_tag="VVN16_000604"
     CDS             complement(3198..3569)
                     /locus_tag="VVN16_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU5572047.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIARKKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3572..4936)
                     /locus_tag="VVN16_000605"
     CDS             complement(3572..4936)
                     /locus_tag="VVN16_000605"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU5572048.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5305..6090
                     /locus_tag="VVN16_000606"
     CDS             5305..6090
                     /locus_tag="VVN16_000606"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU5572049.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6184..6618
                     /locus_tag="VVN16_000607"
     CDS             6184..6618
                     /locus_tag="VVN16_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5572050.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6618..7592
                     /locus_tag="VVN16_000608"
     CDS             6618..7592
                     /locus_tag="VVN16_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU5572051.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7652..7876
                     /locus_tag="VVN16_000609"
     CDS             7652..7876
                     /locus_tag="VVN16_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5572052.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7995..8969
                     /gene="fabK"
                     /locus_tag="VVN16_000610"
     CDS             7995..8969
                     /gene="fabK"
                     /locus_tag="VVN16_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU5572053.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            8962..9882
                     /gene="fabD"
                     /locus_tag="VVN16_000611"
     CDS             8962..9882
                     /gene="fabD"
                     /locus_tag="VVN16_000611"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5572054.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9916..10647
                     /gene="fabG"
                     /locus_tag="VVN16_000612"
     CDS             9916..10647
                     /gene="fabG"
                     /locus_tag="VVN16_000612"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU5572055.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10669..11904
                     /gene="fabF"
                     /locus_tag="VVN16_000613"
     CDS             10669..11904
                     /gene="fabF"
                     /locus_tag="VVN16_000613"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5572056.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11907..12392
                     /locus_tag="VVN16_000614"
     CDS             11907..12392
                     /locus_tag="VVN16_000614"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU5572057.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12389..12811
                     /gene="fabZ"
                     /locus_tag="VVN16_000615"
     CDS             12389..12811
                     /gene="fabZ"
                     /locus_tag="VVN16_000615"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU5572058.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12823..14190
                     /gene="accC"
                     /locus_tag="VVN16_000616"
     CDS             12823..14190
                     /gene="accC"
                     /locus_tag="VVN16_000616"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5572059.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14227..15093
                     /locus_tag="VVN16_000617"
     CDS             14227..15093
                     /locus_tag="VVN16_000617"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU5572060.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15090..15857
                     /locus_tag="VVN16_000618"
     CDS             15090..15857
                     /locus_tag="VVN16_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU5572061.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            16120..16305
                     /gene="briC"
                     /locus_tag="VVN16_000619"
     CDS             16120..16305
                     /gene="briC"
                     /locus_tag="VVN16_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU5572062.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            16323..16774
                     /locus_tag="VVN16_000620"
                     /pseudo
     CDS             16323..16774
                     /locus_tag="VVN16_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16787..17023
                     /locus_tag="VVN16_000621"
     CDS             16787..17023
                     /locus_tag="VVN16_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5572063.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(17073..17495)
                     /gene="nusB"
                     /locus_tag="VVN16_000622"
     CDS             complement(17073..17495)
                     /gene="nusB"
                     /locus_tag="VVN16_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5572064.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17488..17877)
                     /locus_tag="VVN16_000623"
     CDS             complement(17488..17877)
                     /locus_tag="VVN16_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU5572065.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17899..18459)
                     /gene="efp"
                     /locus_tag="VVN16_000624"
     CDS             complement(17899..18459)
                     /gene="efp"
                     /locus_tag="VVN16_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5572066.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGITVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            18627..18812
                     /locus_tag="VVN16_000625"
     CDS             18627..18812
                     /locus_tag="VVN16_000625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572067.1"
                     /translation="MEIKEQTRKLAAGCSKHCFEVVDRTDEVSNHIYGNVTLTWSEEI
                     FEEYYISKGRSKFTPTC"
     gene            complement(18837..19880)
                     /locus_tag="VVN16_000626"
     CDS             complement(18837..19880)
                     /locus_tag="VVN16_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEU5572068.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEDFIELT
                     QDKSQIKILVEPE"
     gene            complement(20004..21446)
                     /gene="gatB"
                     /locus_tag="VVN16_000627"
     CDS             complement(20004..21446)
                     /gene="gatB"
                     /locus_tag="VVN16_000627"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU5572069.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKIEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(21446..22912)
                     /gene="gatA"
                     /locus_tag="VVN16_000628"
     CDS             complement(21446..22912)
                     /gene="gatA"
                     /locus_tag="VVN16_000628"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU5572070.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(22912..23214)
                     /gene="gatC"
                     /locus_tag="VVN16_000629"
     CDS             complement(22912..23214)
                     /gene="gatC"
                     /locus_tag="VVN16_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5572071.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            23427..24971
                     /locus_tag="VVN16_000630"
     CDS             23427..24971
                     /locus_tag="VVN16_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU5572072.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            25398..25596
                     /locus_tag="VVN16_000631"
                     /pseudo
     CDS             25398..25596
                     /locus_tag="VVN16_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            25707..25883
                     /locus_tag="VVN16_000632"
     CDS             25707..25883
                     /locus_tag="VVN16_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU5572073.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            25999..26187
                     /locus_tag="VVN16_000633"
     CDS             25999..26187
                     /locus_tag="VVN16_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5572074.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            26344..26709
                     /locus_tag="VVN16_000634"
     CDS             26344..26709
                     /locus_tag="VVN16_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU5572075.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            26712..28379
                     /locus_tag="VVN16_000635"
     CDS             26712..28379
                     /locus_tag="VVN16_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU5572076.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            28579..30279
                     /locus_tag="VVN16_000636"
     CDS             28579..30279
                     /locus_tag="VVN16_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU5572077.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            30272..30748
                     /gene="ilvN"
                     /locus_tag="VVN16_000637"
     CDS             30272..30748
                     /gene="ilvN"
                     /locus_tag="VVN16_000637"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU5572078.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            30814..31836
                     /gene="ilvC"
                     /locus_tag="VVN16_000638"
     CDS             30814..31836
                     /gene="ilvC"
                     /locus_tag="VVN16_000638"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5572079.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            31943..32179
                     /locus_tag="VVN16_000639"
     CDS             31943..32179
                     /locus_tag="VVN16_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572080.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            32417..>32586
                     /locus_tag="VVN16_000640"
     CDS             32417..>32586
                     /locus_tag="VVN16_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5572081.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLD"
BASE COUNT         9486 a         6139 c         7446 g         9515 t
ORIGIN      
        1 tgataaacta atataacaac gaaaatctta tgagagaata aagctttatc ttagataaac
       61 ttaggaatca ggattttata agattttcca ataatattag tgtcaataat aagaaaagag
      121 gtatcttatg acaacattta ctatccatac agtagagtca gcaccagcag aagtgaaaga
      181 aattcttgaa acagtagaaa aagacaacaa tggctatatt cccaacctaa tcggtctctt
      241 ggccaatgcc ccgactgttt tagaagccta ccaaattgtc tcatctatcc accgtcgcaa
      301 cagcctgaca cccgttgagc gtgaagtggt gcaaatcacg gcagccgtga ccaatggttg
      361 tgccttctgt gtcgcaggtc acacagcctt ttccatcaaa caaatccaga tgaatgatga
      421 cttgattcaa gctcttcgca atcgtactcc aattgaaaca gatcctaaat tggataccct
      481 agctaagttt accttggcag ttatcaatac caagggtcgt gtaggagatg aagccttgtc
      541 tgagttttta gaagctggct acactcaaca aaatgccttg gatgtggttt ttggtgtcag
      601 cctagcaatc ctctgtaact atgccaacaa cttagctaat acaccaatta atccagaatt
      661 gcaaccttat gcctaattca tatctgagta aaatgaagtc tgaaataagc ggacttcgtt
      721 tttttaaatc ctcatttaag cgcttttatg ttatactgaa actaacatga ttattggagg
      781 ttaggatgaa aaaactcccc ttagtatttt caggttgttt gctaggtttg gcaggagctg
      841 gaaatcttat tttagatacg ttgccggttc tatcccatct ttttagtctg actggtttgg
      901 ttttatggat ttactttcta attctgcatc tctttaattg gaaagaaacc aagcaagaat
      961 tgaccaagcc ccctcttttg tcaggaatgg caacctttcc tatggctggg atgattttat
     1021 cgacctatgt ctttcgcgta ttctcttatc ttcctttggt agcacaaggg atttggtggt
     1081 tttcatttct cttggatttg accttgattg ctggttttac catcaagttt gcttgtccag
     1141 ggcggagggt tcatgccact ccaagctgga cggttctcta tgtggggata gcagtggctg
     1201 ccttgaccta tcctctggta ggtattatcg aaattgccta tgcgaccttg agttttggtt
     1261 ttctcttgac cttctatctc tatcccctta tttatagcga tttaaagaaa catccactcc
     1321 cactagcctt gcttggacaa gaaggaatct actgtgctcc tttctctcta ctcttggctt
     1381 ctctagttcg agtaggagga accagcctgc cgacttgggt cttgattgtc atgattttgg
     1441 cttctcaatc cttctttttc tttgttttaa ctcgtctgcc caacatttta aaacaaggtt
     1501 ttcaaccagc cttctcagcc ctcaccttcc caaccattat cacagcgacc tcgctcaaga
     1561 tggctcaggg aattttgaaa cttccatttc tggattacct ggtattggct gaaaccatta
     1621 tatgcctaac tattttattc tttgtactag gtgcttatct gatttggtta cgaaaaaagg
     1681 tctagctaga aatagctaaa ccttattttt tatggtttga tgacttcagc tccacccatg
     1741 tatggacgaa gtgcttctgg gatggtcaca gaaccatctt cattttggta attttcaaga
     1801 attgcagcca ctgtacgtcc aactgcaagt ccagaaccgt tcaaggtatg aaggagtttc
     1861 accttgccat ctgcttcatc acggtaacgg atttgggcac gacgggcttg gaaatcttct
     1921 gtgtttgaac agcttgagat ttcacggtaa ttgttttgtg ctggaatcca cacttccaag
     1981 tcgtaagtct tcgcagctga gaagcccata tctccagtag agagagcaac gacacggtat
     2041 ggaaggttga gtttttgaag aatgttttca gcgttggctg tcattttttc caattcttcg
     2101 taagattctt ctggtttggc aaatttgacc atttcaacct tgtggaattg gtgcaaacgg
     2161 atcaagccac gcgtatcacg accggcagag ccagcctcag aacggaatga cggactcata
     2221 gcagtgaagt agattggaag atctttgccg tctaagattt catcacggta gtagtttgtc
     2281 agaggaactt cagcagttgg aatcaagaca aagttggtat cgctgagttc aaaagtatct
     2341 tccttaaatt ttggatactg accagtacca aacatagaat catggttgac tatgtaaggt
     2401 gtgatgactt cagtatagcc ttcttttcca tgttcatcca acataaagtt gtagatagca
     2461 cgttccaaac gagcaccgag gcctttatag aagaggaagc gagcgcctgt taccttacca
     2521 ccgcgttccc agtcaaggat accaaggtct tcaccgagat cccagtgagc tttaggttcg
     2581 aagtcaaact cgcgtggagt accccaacgg cgaacttcca cattgtcgtc ttcgtcagcc
     2641 ccaacaggaa cgctgtcagc tgggatattt ggaagagtcg ttgtaaattc tgtcaattta
     2701 gcatcgattt ctgccaattc agcatccaag gctttaacct cagcagatag attttgcatg
     2761 gcagcaatct tgtcatctgt attttccttg ttgcgcttag cttgggcaat ctcagcagaa
     2821 actgtgttac gttctgcttt gagagtttca accttgacca agatgttacg acgtttagca
     2881 tcgatttttt tcatttcatt caagacagca gcatctacac cacgtgtagc taatttttct
     2941 gcgacagctt caaaatctgt acgaatacgt ttgatatcta acataagaac tcctttatga
     3001 aaaaagcacg cctgacaaaa cgttggagtg gcagggccac ggttccatcc aacttcacag
     3061 gtgtgcactt gattgtgtat gtaattgtta ctaacggtag aatttcacct atccctccta
     3121 tctgctcgca gtacccgcag actttctgaa agaagaagat aacctactta tccgttgcta
     3181 tgattatact aaagtttcta cttttttgca aatagatttt taaatttttg gctaattgtc
     3241 tgaatcaggg tcggaagttt gacgaccttg tcattgccta gttttttgcg tgcaattttg
     3301 agaatggcac ctgagtcttt tgaagcaaag aggaattttc ctttgtctgt aaagacttcg
     3361 aagtggcggc tgattttgcg tccagtgaca ttggctccaa tctgattgat atggctccaa
     3421 ggaatctgga taaattgttc gacattgaca tctgggtaaa attccaaagc ctgatctccg
     3481 acaaggaatt tcccaacttt cccagcgata gagaggtagg aagtgcctgt cgtactgagg
     3541 agtactgttt tgttaagtga ttgggccatg cttagtcttc cttactttct ccaaaaaagg
     3601 cattgtagag ggctttaatt gctgctttct cttggtcttt attgacaaca aacataatag
     3661 aaacttcact agaaccttga gacatcatct ggatgttgat tttgttttca gatagagcgc
     3721 gtgtcgcagt agcagtcact ccgatgtggc tcttcatttt ttcaccaaca atcataatga
     3781 tagaaaggtc gtgttcgatt tctgcatgat ctactttagc cttttgaacc aactgacgca
     3841 ggatttcttc ttccttgatg ggagttagtt ggcgagaacg gagaatgata gaaagatcgt
     3901 cgatacctgt tggcatatgt tcccaaccga tgttaagttc ttccaggatt tgcagaacct
     3961 tgcggccaaa tccaacctca cggttcatga ggtatttcga catgttaatg ctgacaaagc
     4021 ctgagtcacc agcaattccc acaactggaa attcatcatt actgtgtttt agaacgatac
     4081 gagtacctgg atggtcaggg ttgttggtat tcttgataac cagaggaatt tttccacggt
     4141 aggcaggaag aagagcctcg tcatgaagga ctgagaagcc tgcataggcc aactcgcgca
     4201 tttcacggta ggtcaactca ggaatcgagt gtggttggtg gataatacca gggtgggctg
     4261 caaagatacc atcaacgtcc gtaaagtttt catagaggtc agctttgaca ccagcagcaa
     4321 tgatagaacc tgtaatatca gaacctccac gtgagaaagt acagatttga ttttccttag
     4381 tgacaccaaa gaaaccagga atgacaagga cttcattggt gtttgtcaat tcttcaatct
     4441 tgtcataact tgatggaatg atgcgagcgt gaccaggttc acttgtgacc acaatcccag
     4501 cttctctagg gtgcatatag cgtgcatcga taccattttg gttaaagtag gcagcaatca
     4561 atttggcatt gttattttca ccggctgcta ggaaagtatc gtagagaaat tcattttctt
     4621 caataggaag agtggccaag gcgtgaatac ttttagaaat tttttctagc acagctggtt
     4681 ttagtcccaa ttcactaacc atagcagcat agcggtcgat aatccagctt tggctcttgc
     4741 taatatcgtt acccgcaaca tagtcgcggt agtatttaat cagggcatcc gtaaccttag
     4801 tatcttcagc attgcgttta ccaggcgcag aaacgactac aaaacgacgc tctgaatcgc
     4861 ttttgacgat gtttaaaact ttttctaatt gactagcaga ggcaagagaa ctacctccaa
     4921 atttaacaac tttcataaga actcctaaag taagtatttt atacgattat agcagaaaga
     4981 aaggcttttt tcaatgaaga aaataagatg ctttctagcc taaagcgagc ctttcttatc
     5041 ggctgagaca ctttcagtcg attttttctt gctttctgct cctaaaaaag atatactttg
     5101 acagtgaaat aatttaacac tcaatgaaat caaagagcaa actaggaagc tagccgcaga
     5161 ctgctcaaag cactgctttg aggttgtaaa taagactgac gaagtcagct caaaatactg
     5221 ttttgaggtt gcagatggaa gctgacgcgg tttgaagaga ttttcgaaga gtataaactg
     5281 cttataaaat aaaaaggagc cctgatggaa cacattattt atcagcttga agaggacttg
     5341 gcaatcctta ccttgaaccg tcctgaggtc gcaaatggtt ttcatattcc catgtgtgag
     5401 gagattttag aagctctgac tttggcagaa gaaaatccag ctgtgcattt tatcttaatc
     5461 aatgccaatg ggaaggtctt ctcagttggg ggagatttgg tagagatgaa gcgggcagtg
     5521 gatgaggatg atattccatc attgacaaaa atcgcagagt tggtcaatac gatttcttat
     5581 aaaatcaagc aaatagccaa acctgtttta atggaagttg acggtgctgt tgcaggtgcc
     5641 gcagcgaata tggctgttgc ggcagatttc tgtttggcga cggataaggc taagtttatc
     5701 caagcctttg ttggtgttgg tttggctcca gatgcagggg gaattcatct cttgagccgc
     5761 agtatcggtg tgacgcgtgc tgctcaatta gctatgacag gagaggcttt aacggcagaa
     5821 aaagccctag aatgggggtt ggtttaccgc gtttctgaag ctgaaaaact tgaaaagacg
     5881 agagaacagc ttcttaaaaa attaagacgt gcttcaagta attcctatgc tgccattaag
     5941 aagttggttt gggagagcca atttaaagac tggcagggtt acgctacttt agaattgaac
     6001 ctacagaaat ccttagctca aacagaggat ttcaaagaag gagtgcgagc tcattcggag
     6061 agaagaagac ctaaatttac aggaaaataa aaaatacttg catcattctt tgaattttga
     6121 tataattctt tctgtcaaat gttttgattg taaaagtttt ttgaagaagg agggatatga
     6181 aaaatggact accaacgaat caatgaatat ttaacatcta tatttaacaa tgtccttgta
     6241 attgaggaag tgaacttgag aggtagtcgt tttaaggata tctccatcaa agaaatgcat
     6301 acgattgatg tcatcggtaa ggctccagat gtgactccaa gtcaagtgtc aaaagagttg
     6361 atggtaactc ttggaactgt tacgacaagt ttgaacaatt tagagcgtaa gggctacatt
     6421 gagcgagttc ggtcagaaca ggatcgtcgt gtggtgcatc tgcatttgac aaagaagggt
     6481 cgcttgattc atagactgca taaacgcttc cacaaggcca tggtagaaaa aattattgat
     6541 ggcatgagcg aggaagaaat tgctgtcatg ggtaaaggct tgactaatct ttaccaattt
     6601 ttggaggatt tgaaataatg gcttttgcaa aaataagtca ggttgctcat tatgtgccag
     6661 agcaagtggt tacaaatcac gacttggctc agattatgga taccaatgat gagtggattt
     6721 caagtcgaac gggaatacga caaaggcata tttcaagaac agaatctacc agtgatttgg
     6781 ctacagaggt tgctaagaaa ctgatggcaa aagctggaat aacaggagaa gaactggatt
     6841 ttatcatcct agctaccatt actccagatt cgatgatgcc ctctacagct gctcgtgttc
     6901 aagctaatat tggcgctaat aaagcctttg cttttgactt aaccgcggct tgcagtggat
     6961 ttgtatttgc tctttcaact gctgaaaagt ttatcgcttc tggtcgcttt caaaaaggct
     7021 tggtgattgg tagtgaaacc ctctctaagg cagtcgattg gtcggatcga tcaacagctg
     7081 tgttgtttgg agatggtgct ggtggtgtct tgttagaagc tagcgagcaa gagcatttct
     7141 tagctgagag tctcaatagc gatggaagtc gcagcgagtg tttaacttat gggcattcag
     7201 gtttgcattc tccattttca gatcaagaaa gtgcagattc gtttttgaag atggatggac
     7261 gcacagtctt tgattttgcc attcgagatg tagccaagtc tatcaagcag actattgatg
     7321 aatctcctat agaggtgaca gacttggatt atctgctact tcatcaagcc aatgaccgta
     7381 ttttggataa gatggctaga aaaattggtg ttgaccgagc caaacttcca gccaatatga
     7441 tggaatatgg caataccagt gcagccagta tcccgatttt actttcagag tgtgtagaac
     7501 aaggtctcat ccctttagat ggtagccaga ctgttcttct atcaggcttt ggtggaggct
     7561 tgacctgggg cacgctcatt cttacaattt aggtaattat gtggtgaaca cattgttata
     7621 aaaaaactat ttattttaaa ggagtcctat catggcagta tttgaaaaag tacaagaaat
     7681 tatcgttgaa gaacttggaa aagacgcatc agaagtaaca cttgaatcaa cttttgatga
     7741 tttggacgca gattcattgg acttgttcca agtaatctca gaaatcgaag atgcttttga
     7801 tatccaaatc gaagcagaaa atgacttgaa aacagttggt gacttggttg cttacgttga
     7861 agagcaagca aaataagcag aatattagta gaaggagtag ggagaaaccc actccctctt
     7921 gtttaggtaa tagtttgact gtcaaattat ggtgaaatcg aactattacg taagcaagga
     7981 aggttggagg cactatgaaa acgcgtatta cagaattatt gaagattgac tatcctattt
     8041 tccaaggagg gatggcctgg gttgctgatg gtgatttggc aggggctgtt tccaaggctg
     8101 gaggattagg aattatcggt gggggaaatg ccccgaaaga agttgtcaag gccaatattg
     8161 ataaaatcaa atcattgact gataaaccct ttggggtcaa catcatgctc ttatctccct
     8221 ttgtggaaga tatcgtggat ctcgttattg aagaaggtgt taaagttgtc acaacaggag
     8281 caggaaatcc aagcaagtat atggaacgtt tccatgaagc tgggataatc gttattcctg
     8341 ttgttcctag tgtcgcttta gctaaacgca tggaaaaaat cggtgcagac gctgttattg
     8401 cagaaggaat ggaagctggg gggcatatcg gtaaattaac aaccatgacc ttggtgcgac
     8461 aggtagccac agctatatct attcctgtta ttgctgcagg aggaattgcg gatggtgaag
     8521 gtgctgcggc tggctttatg ctaggtgcag aggctgtaca ggtggggaca cggtttgtag
     8581 ttgcaaaaga gtcgaatgcc catccaaact acaaggagaa aattttaaaa gcaagggata
     8641 ttgacactac gatttcagct cagcactttg gtcatgctgt tcgtgctatt aaaaatcagt
     8701 tgactagaga ttttgaactg gctgaaaaag atgcctttaa gcaggaagat cctgatttag
     8761 aaatctttga acaaatggga gcaggtgccc tagccaaagc agttgttcac ggtgatgtgg
     8821 atggtggctc tgtcatggca ggtcaaatcg cagggcttgt ttctaaagaa gaaacagctg
     8881 aagaaatcct aaaagatttg tattacggag ccgctaagaa aattcaagaa gaagcctctc
     8941 gctggacagg agttgtaaga aatgactaaa acagcctttt tatttgctgg tcaaggtgcc
     9001 cagtatctag ggatgggacg ggatttctat gatcagtatc cgattgttaa agaaacgatt
     9061 gatcgagcga gtcaggtgct cggttatgat ttgcgttatc tcatcgatac ggaagaggac
     9121 aaactcaatc agacccgcta tacgcaacca gccattctag cgacttcggt tgctatctac
     9181 cgtttattgc aagaaaaggg ctatcagcct gatatggttg ctggtttgtc tcttggagaa
     9241 tactctgcct tggtggcaag cggcgccttg gattttgaag atgcggttgc cttggtagct
     9301 aagcgtggag cctatatgga agaagcgact cctgctgact ctggcaagat ggtagcagtt
     9361 ctcaatacgc cagtagaggt cattgaagaa gcctgtcaaa aagcttctga acttggagtg
     9421 gttactccag ccaactataa cacacctgca caaatcgtca ttgctggaga agtggttgca
     9481 gttgatcgag cggttgaact tttgcaagaa gcaggtgcca aacgcttgat tcctcttaag
     9541 gtgtcaggtc cctttcacac cgctctcctt gagccagcta gccagaaact agctgaaact
     9601 ctagctcagg taagtttttc agattttact tgtcccctag tcggcaatac agaagctgct
     9661 gtgatgcaaa aagaggacat tgctcagctc ttgacgcgtc aggtcaagga acccgttcgt
     9721 ttctatgaaa gtattggggt catgcaagaa gcaggcataa gcaactttat cgagattgga
     9781 ccggggaaag tcttgtcagg ttttgttaaa aaaattgatc aaactgctca cttagctcat
     9841 gtggaagatc aagcgagttt agtagcactt ttagaaaaat agactaaaat aagtagaagt
     9901 tttgaaagga aaaaaatgaa actagaacat aaaaatatct ttattacagg ttcgagtcgt
     9961 ggaattggtc ttgccatcgc ccacaagttt gctcaagcag gagccaacat tgtcttaaac
    10021 agtcgtgggg caatctcaga agaattgctc gctgagtttt caaactatgg tatcaaggtg
    10081 gttcccattt caggagatgt atcagatttt gcagacgcta agcgtatgat tgatcaagct
    10141 attgcagaac tgggttcagt agatgttttg gtcaacaatg cagggattac ccaagatact
    10201 cttatgctca agatgacaga agcagatttt gaaaaagtgc tcaaggtcaa tctgactggt
    10261 gcctttaata tgacacaatc agtcttgaaa ccgatgatga aagccagaga aggtgctatc
    10321 attaatatgt ctagtgttgt tggtttgatg gggaatattg gtcaagctaa ctatgctgct
    10381 tctaaggctg gcttgattgg ctttaccaag tctgtggcac gcgaggtcgc tagtcggaat
    10441 atacgagtca atgtgattgc tccaggaatg attgagtctg atatgacagc tatcttatca
    10501 gataagatta aggaagctac actagctcag attccgatga aagaatttgg gcaggcagag
    10561 caggttgcag atttgacagt atttttagca ggccaagatt atctaactgg tcaagtgatt
    10621 gccattgatg gtggcttaag tatgtagcga aagctagagg tgaaaagaat gaaactaaat
    10681 cgagtagtgg taacaggtta tggagtaaca tctccaatcg gaaatacacc agaagaattt
    10741 tggaatagtt tagcaactgg gaaaatcggc attggtggca ttacaaaatt tgatcatagt
    10801 gactttgatg tgcataatgc ggcagaaatc caagattttc cgttcgataa atactttgta
    10861 aaaaaagata ccaaccgttt tgataactat tctttatatg ccttgtatgc agcccaagag
    10921 gctgtaaatc atgccaatct tgatgtagag gctcttaata gggatcgttt tggtgttatc
    10981 gttgcatctg gtattggtgg aatcaaggaa attgaagatc aggtacttcg ccttcatgaa
    11041 aaaggaccca aacgtgtcaa accaatgact cttccaaaag ctttaccaaa tatggcttct
    11101 gggaatgtag ccatgcgttt tggtgcaaac ggtgtttgta aatctatcaa tactgcctgc
    11161 tcttcatcaa atgatgcgat tggggatgcc ttccgctcca ttaagtttgg tttccaagat
    11221 gtgatgttgg tgggaggaac agaagcttct atcacacctt ttgccatcgc tggtttccaa
    11281 gccttaacag ctctctctac tacagaggat ccaactcgtg cttcgatccc atttgataag
    11341 gatcgcaatg ggtttgttat gggtgaaggt tcagggatgt tggttctaga aagtcttgaa
    11401 cacgctgaaa aacgtggagc tactatcctg gctgaagtgg ttggttacgg aaatacttgt
    11461 gatgcctacc acatgacttc tccacatcca gaaggtcagg gagctatcaa ggccatcaaa
    11521 ctagccttgg aagaagctga gatttctcca gagcaagtag cctatgtcaa tgctcacgga
    11581 acgtcaactc ctgccaatga aaaaggagaa agtggtgcta tcgtagctgt tcttggtaag
    11641 gaagtacctg tatcatcaac caagtctttt acaggacatt tgctgggggc tgcgggtgca
    11701 gtagaagcta tcgtcaccat cgaagctatg cgtcataact ttgtaccaat gacagctggg
    11761 acaagtgaag tatcagatta tatcgaagct aatgtcgttt atggacaagg tttggagaaa
    11821 gaaattccat acgctatttc aaatactttt ggttttggag gccacaatgc agttcttgct
    11881 ttcaaacgtt gggagaatag ataagtatga atttaaacga tattaaagac ttgatgactc
    11941 aatttgacca gtcaagtttg agagaatttt cttataaaaa tgggacggat gagttgcagt
    12001 ttagcaagaa tgaagcaaga cctgtgcctg aagttgcaac tcaagtcgct ccagcacccg
    12061 ttctagcaac accgagtcca gtagctccta catctgctcc agcagagact gtagcagaag
    12121 aagttccagc tccagctgaa gcaagtgtgg ctactgaggg aaatcttgta gagagtccac
    12181 ttgttggagt ggtttacttg gctgctggac cagataaacc tgccttcgtt acagttggtg
    12241 atagtgtcaa aaaaggtcaa acattggtaa ttatcgaagc catgaaagtc atgaatgaaa
    12301 tcccagctcc taaggatggt gtggtaacgg aaattctcgt ctctaacgaa gaaatggttg
    12361 agtttggtaa aggattggta cgtatcaaat gatcgatatt caaggaatca aagaagctct
    12421 tccccaccgt tatcctatgc ttctagtgga ccgtgtcttg gaagtgagcg aggataccat
    12481 tgttgctatc aaaaatgtga ccattaacga acctttcttt aacggccact ttcctcaata
    12541 cccagttatg ccaggtgttc tgattatgga agccttggcg caaactgctg gtgtgttgga
    12601 gttatcaaaa cctgaaaata aaggaaaact ggtcttttac gctggtatgg acaaggttaa
    12661 gttcaagaag caagttgtac caggcgacca attggttatg acagcgactt ttgtaaaacg
    12721 tcgtggcacc atagctgtgg ttgaagcaaa ggctgaagtg gatggcaagc ttgcagccag
    12781 tggtatcctt acttttgcaa ttgggaacta aggaggttct ccatgtttcg aaaaatttta
    12841 attgccaatc gtggtgaaat tgcggttcgt attatccgtg cggcacgtga attggggatt
    12901 gcgacggtag cggtttattc aactgctgat aaggaagctc ttcatacgct tttggcagat
    12961 gaagcagttt gtattggtcc tggcaaggca acagagtctt atctcaatat taatgcagtt
    13021 ctatcagctg cagtcttgac tgaggcagaa gctattcacc ctggttttgg atttctcagt
    13081 gaaaattcca aatttgcgac catgtgtgaa gaagtaggta tcaagtttat cggtccatct
    13141 ggtcatgtta tggatatgat gggggataag atcaatgcgc gtgctcagat gattaaagca
    13201 ggtgtgcctg ttataccagg ttcagatgga gaagtgcata actctgaaga agctttgatt
    13261 gttgctgaaa aaattggcta tcctgttatg ctcaaggctt cagcaggtgg aggtggtaaa
    13321 gggattcgta aggttgaaaa accagatgac ctcgtttctg cctttgaaac tgcctctagt
    13381 gaggccaagg ccaattatgg caatggtgcc atgtacatag aacgggttat ctatccagct
    13441 cggcacattg aggttcaaat cctaggtgat gagcatggac atgtgattca cttgggtgag
    13501 cgggattgtt ctcttcaaag gaataaccaa aaggttttgg aagaaagtcc ctcgattgca
    13561 atcggaaaaa cgctgcgtca tgaaataggt gctgctgctg ttcgagcggc agagtttgtt
    13621 ggctatgaga atgcaggaac cattgaattt cttcttgatg aagcaagtag caatttctat
    13681 ttcatggaga tgaatactcg tgttcaggta gaacatccag taacagagtt tgtttcaggt
    13741 gttgatatcg ttaaggaaca gatttgcatt gcggcaggtc agcctttgtc tgttaagcaa
    13801 gaagatattg tcctacgcgg tcatgccatc gagtgtcgta tcaatgcaga aaacccagcc
    13861 tttaactttg ctccaagtcc aggtaagatt actaatctct atctgccaag tggtggagtt
    13921 ggcttgcgcg tggattcagc agtttatcca ggttatacca ttccgcctta ttatgatagt
    13981 atgattgcca aaatcatagt acacggcgaa aatcgttttg acgccttgat gaaaatgcaa
    14041 cgtgccctct atgaattaga gattgaagga gtgcagacca atgcagattt ccagcttgac
    14101 ctcatttcag atcgcaatgt cattgctggg gattatgata cttccttctt gatggaaacc
    14161 ttcttaccta aatatcaaga aaaagaataa aatgacttta agagtttaaa ccgaaaaggg
    14221 gaatcaatgg ctctatttag taaaaaagat aagtatattc gaatcaatcc caatcgttcg
    14281 gttagggaaa aacctcaagc taagccagag gttccagatg aattattttc ccagtgtcca
    14341 ggctgtaagc ataccatcta tcagaaggat ctgggaagtg aacgtatctg tccgcactgt
    14401 agctatacct ttcgtatttc tgcccaagaa cgcttggctt tgacgattga tatgggaacc
    14461 ttcaaagaat tgtttacagg gattgaaagc aaggatccct tgcatttccc tggttaccaa
    14521 aagaaactgg catctatgcg tgaaaaaaca ggtctgcatg aagccgttgt gacaggaact
    14581 gctcttatta aaggtcagac tgtggctctt gggattatgg attctaactt tatcatggct
    14641 tctatgggta cggttgtagg tgaaaaaatc actcgtttgt ttgagtatgc gactgtcgaa
    14701 aaattgccag ttgtcctatt cacagcctct ggtggagccc gtatgcagga aggaatcatg
    14761 agtctcatgc agatggctaa gatctctgcg gcggttaaac gccattcaaa tgctggtctc
    14821 ttttacctga ccattttgac agatccaacg actggtggtg tgacagcttc tttcgctatg
    14881 gaaggcgata tcattctggc tgaaccacag agcttggttg gttttgctgg acgtcgtgtg
    14941 attgaaaata cggttcgtga aagcttgcct gaggatttcc aaaaggcaga attcctatta
    15001 gaacatggct ttgtggatgc tattgtcaaa agaagagact taccagatac gattgctagc
    15061 ctagtcagat tgcatggagg gagtcctaga tgaatattgc aaaaatagtc agagaagcgc
    15121 gtgagcagag tcgcttgaca accttagact ttgctacagg catttttgat gaatttatcc
    15181 aattacatgg tgaccgttct tttcgtgatg atggtgcagt tgttggtggt attggttggc
    15241 ttggagacca agctgtaaca gtggttggta tccaaaaagg caagagtttg caagacaacc
    15301 tcaaacggaa ttttggccaa ccacatccag aaggctaccg aaaggcactg cggttgatga
    15361 aacaggctga gaaatttggc cgtccagttg tgacctttat caatacagca ggtgcttatc
    15421 ctggtgtcgg agcggaagaa cgtggtcaag gggaagctat cgctcgcaat ctcatggaaa
    15481 tgagtgacct gaaagttcct attatcgcca ttattatcgg tgaaggtggt tcaggcgggg
    15541 ctctggctct agctgtcgcg gaccgtgtct ggatgctgga aaattctatc tatgccattc
    15601 tcagtccaga aggctttgct tccattttat ggaaggacgg tactcgcgcc atggaagcag
    15661 cagaactgat gaaaatcact tcgcatgaac tgttagaaat ggacgtggtg gataaggtga
    15721 tttctgaagt aggactttct agtaaagaac tgattaagag tgtcaaaaaa gaactccaaa
    15781 cggagctggc tagactttca caaaaaccgc tagaagagtt gctggaagaa cgctatcaac
    15841 gatttagaaa atactaagaa tgactaaatg agactagaaa ggtctcattt tttatggaaa
    15901 aatgcaattc aggttgaaaa attgacagtt gaggagatgg gctataaaat agacaaaaaa
    15961 gatgtatact gtagatgtaa gcttacaaaa atttatagtg tattgaatct ataacagtac
    16021 accttggctg ctaaaatatt tctataaatt aatttgactt tcctgataga gttgttcaca
    16081 tcttatttca aatcactata ttttaagaag gtacttgcaa tgacaggtac agaaacattt
    16141 acagttattt caactgagga cttggagcaa acttcaggtg gtcttgctgt ttgggaagat
    16201 ggatatagta gatggttata ttatagagaa tttgctccct atatgaggca aggggcactt
    16261 aattcttata tagatgcttg gaagtacggc ttccgaacag ggtaatcttt caaaaaagta
    16321 cgatgagaaa cacaaagaaa ctaagacaat ttggaatatt tttgcttatt attttactat
    16381 ctacctattt accacaaaca attggattgt atgtgacgat cattttaggg ttaggagctg
    16441 atgtctattc tcttatttta acagtgggat tagtgggaag tttccttttc tattaatttg
    16501 gaggttaaaa aagaaaaaga gctgggactg gtcatttgtc ttctccttat ttgcaactta
    16561 tgtggtttat cagattctcg gtaatttttg ggcacgctat gctcatttga ttaatcatag
    16621 gaatattcat gatgagtatt ttaccgtggt tctttcaaat ggacaaccaa cctttttatc
    16681 gactatctta tcttttgtcc tgcctgtaat catcggtcct gtttttgagg agactcttga
    16741 tagagggtat tttatgaaca ccttctttcc ctagtccaag tattatttgg atgttatcct
    16801 atctgctatc atctttggga ttagccattt gatactatct cacagagatc caattagttt
    16861 attatattat agtctgattg gtttcttttt tgcccttgtt tatcgttcta ccgacaatct
    16921 gagattaacg attctctgcc atagtttttt taactttctc aatcatgcaa aacctatctg
    16981 gatttttgtt tacaattata tctattatca tttttttaga tagtgggagg caaagaaaaa
    17041 agtgtttgat atctatcaaa cactttttct ttttattgtt cttctgttac aaactggctg
    17101 agcagtccat tgataaaacg ggcagatttt tgatcggaga agtcctttgc aagctcgata
    17161 gcttcattaa cagcaaccag ctgaggagtg tcaaatgaag tgatttcaaa gactcccaag
    17221 cgaaggaggt ttctctccac gagcgttaag cgttcaatgg tccaacctgc ttttaaatgc
    17281 tgagtgattt gcttatctag ttcttccttt ttagcttgaa caccagaaac gaggtctatc
    17341 aaaaaggctg gaagttgtac atccgtatct tcacgatcat gagtataggc gaaacgacaa
    17401 gcagtttcga catccgtacc gaactcaagg ctcatgagag cttgaaaagc gcatttacgg
    17461 agttggcgtc tagattctaa tagtggacta gtcattgagg aagtcctcgt caaatagatc
    17521 tttcaattct ggttttggtg ttttatctgg gacgatacct gcaacgtgaa tattgatagc
    17581 agcgagttct acatcagcca tattacggac ggcatctttg acagctttct ggatagcaac
    17641 cgctacctta ggaactttta ctccgtactc aaggtagaga tagatatctg ctgtgagttc
    17701 ttcgtccacg tttttaagat aaatgccacg gccgagtgaa agttttgaaa gggtatcaga
    17761 cactgatctg tttgaaaaag agtgaacacc ctctaccttt gcagtagcga tagcaatgat
    17821 tttttcaagt acacgtgggg cgataacgat ttcgccaagt tgttcttcaa ttcccataga
    17881 atgacctctt tctagagatt aggcacgaga aacgtaagtt ccttctgcag tgttgataac
    17941 gagtttttgt cctgcttcga tgaagtctgg aacgtttacg acaagtccag tttccatcgt
    18001 tgctggttta ccagaacctg taacagtagc acctttgata gatggttgag tttcagcaac
    18061 tgtcaactca acagtagtag gaacggtgat accgatcact tcagttccgt agaattggat
    18121 tttcacatca gagttttcaa ggatgtaaag caattcgttt tcaacattga ctacagggat
    18181 ttcgtattgg tcataagttt ctgtattcat gaagtatgct gtgtcatcca ttttgtacaa
    18241 gtattgagct gggacagtct cgataatagc ttgttcaaat ttttcctctg gacggtagct
    18301 tgtgtcaaat gtagaaccag tacggacatc acgcaatttc atacgcatga tcgtgtttcc
    18361 tttacctggt ttgtggtgac tagcttccaa aacgcgaatc aatttgccgt cagctgtttc
    18421 aaaggtcata ccagctttta atttacttgc ttcaatcatg tttttacctc tttaataaat
    18481 attattactc ttcattttac cataaaatac tctggaaata aagccaaaat agttattggg
    18541 agttggtagg agcgaaaaag aatattggct agtcaggtgg agtggttaat gttaaagagt
    18601 atccactata tttcttctca tactcaatgg aaatcaaaga gcaaactagg aagctagccg
    18661 caggctgctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaaccata
    18721 tatacggtaa tgtgacgctg acgtggtctg aagagatttt cgaagagtat tatatttcaa
    18781 aaggccgaag taagtttact ccgacctgtt agtattgtct taaaaagatt gattttttat
    18841 tctggttcta ccaaaatttt aatttgtgac ttatcttgag ttaattcgat aaagtcttct
    18901 tcgacgatat tttccaattt aattttctta gtaactaatt tttcagcaga gaagtagcct
    18961 tgttccatca attctaatac tttagggaag atatgacgat aagcaatgat tccttttagt
    19021 gtcttttctt ggattgcaaa ttcattcgga ttaatatttg cttctcgttc ccagatagag
    19081 accaccatgc actcacctgc tttatgtacg gcagctaagg cttgtcctaa aactactggc
    19141 acacccgtta cttcataaga aacatctaca ccaccgtttg ttaaacgatg aatagcttca
    19201 acagctgttt caccttcttc cggacggaca acgatagctc ctaactcttc agcttttgct
    19261 tgacgttcag gtgatagttc aacagcataa atcttagaag caccagctgc acgaagagct
    19321 tctacaatta atagaccaat cgggcctaaa ccaaagacaa cagctgtatc gccagccttc
    19381 aaagaagatt gacgaacagc atagactgca acagcagcgg gttcagtaag agcagcttgc
    19441 tcatagctca aactatctgg aatgacatgt actaagtcac cgtctaaaac acaatatttg
    19501 gcaaatccac cgtctgcagc caaaccgaca aaattaaggt ttggatccaa attataatca
    19561 ccaactaagt tatttttagc tagaattggt tccacagcaa cacgatcacc aactttaaca
    19621 cgggtaacat cgcttccgac ttctatgatt tcaccagaaa attcatgtcc cagtgttacc
    19681 ggtgcttttt gaccagaata gacgtgctca tctgttggga taaagatagg tccatctaaa
    19741 aattcatgga ggtcagtacc acaaattcct gtgaacttaa ctgcaacttt tacttggtgc
    19801 ggaagtactt caggaacttc tatttcttga atacgaacat ctttcgctgc atgccagcga
    19861 gctgccttca ttgtagccat ataaataact ccttattgtt gcgattttta gttttcttgt
    19921 ttggaaactt tacacgcaat attgcaaacg ttttctttct atttgtcaag aaaaaaccaa
    19981 ccttagggct ggttttgttc tgtctagttt tctttcaact tcgccaattc ctgtgcaagt
    20041 agtttaaggg caacttgtgg gttggcttgg cctttggttg ccttcataag gaatcctgtg
    20101 aaggccttgt ctgcattacg tttgcctgac ttgaagtcgg caacagcagc ttcgttatcc
    20161 gcaaagactt ggtggataat tggaatcaaa acagctggat ctgaaatttg aaccatacct
    20221 gctttttcca cgtattcacg cgcgccaccg ccatttttag ctagatggac aaagactttc
    20281 ttggcaatct tagatgaaat agtaccgtct tcgatgatgg caatcatttc aaccaagttt
    20341 tctggtgtca attcgatttg ttccagtgtt ttaccttcag cattcaagaa ctgagcgact
    20401 tccccttgga gccagttaga gacttgtttg gcatcaccac ctagggcaac agctttttca
    20461 aagaagtcag aagtgacttt attagcagtc aactgactag catcgtagtc tgataaacca
    20521 aggtcagata cataacgcgc acgacgttct tttggaaact ctggcaactc agtccgcatt
    20581 tcctcaatcc actcgtcaga aatttcaaag aggggtaggt ctggttctgg gaagtagcgg
    20641 tagtcagcag ccccttcctt gacacgcatg aggatggttg ctttattcgc ttcatcgtaa
    20701 cggcgtgttt cttggcggat ttgaccacct gagcgaagaa tttcagcctg gcgttggact
    20761 tcgtattcaa gacctttacg aacgtttgag aaggagttga ggttcttcaa ttcagtcttg
    20821 gtaccgaatt tctcttgacc ataaggacga agggagatgt ttgcatccac acgcatcgaa
    20881 ccttcttcca tcttaacgtc agaaatgcca gcgtactgga taacttcctt gagggctgtc
    20941 agataagcat aggcctcctc tggcgaacgc atatctgcct cagatacaat ctcaatcaag
    21001 ggaacccctt ggcggttgag gtcaacataa gagtagccat ctgtaccatg ggtgttttta
    21061 ccagcgtctt cctctaggtg ggcacgttcg ataccgattt tcttggtcgt accgtcttct
    21121 attttgactt caatccagcc attatatccg attggttcat caaactgaga aatttggtag
    21181 gctttggggt tatcaggata gaagtagttc ttgcggtcaa agtgcatctt tttgtggatg
    21241 tccatgttga gggcaagagc agccttgata ccggcatcaa caaccccttt attgagaact
    21301 ggtagaactc ctgggaaaga ccagtcaatc acgttagtgt tggcattttg gtcatttcca
    21361 aagtgggcag aagtaggtga gaagattttt gaattggtgt tgagctctac gtggacttca
    21421 agtccgatga ctgtttcaaa gttcattagt tgtcacctcc aaaaatcacg ggttgttgtt
    21481 tgtggtagtc tgttgttgct tcaaaagcag cagcagcttg gtaaatggtt tcctcagagt
    21541 acttgggacc aatcaattgg agtccgacag gtagaccttg agagaatcca gcaggaatcg
    21601 aaattccagg cagtcctgcc aagtttacag gtatggtcaa taggtcggct aagtacatgg
    21661 caactgggtc atggttgaga gaatccaagt cataggcaac acttggagca gttggaccca
    21721 aaatcaaatc gtaatccgcg aagacttttt cgaaatcttg aatgatgagg gtacggactt
    21781 gaccagcctt tttgtagtag gcatcatagt aacctgatga aagactgaaa gtacccagca
    21841 tgatacgacg ttttacctct tcaccaaaac cttggcttcg gctgtttaca tagatttcat
    21901 caaggttggt tgcatcttct gcgcgatagc cgtaacggat accgtcgaag cgttgcaagt
    21961 ttgatgaagc ttctgatgaa gcgatgatgt aataaacggc aacaccgtat ttagagtgag
    22021 gaaggctgac ttcttcgacg atagcaccca atttttcaaa gtgtttggcc gcgtttaaga
    22081 ttgtttcctt aacctctgga tcaattcctt cgcctaggta ttccttaggc aaagcgattt
    22141 tcataccctt gatgtcttgg ccgatttttg aagtaaagtc ggcgatgcgg acaggagcag
    22201 aagtagagtc tttagcatct tcgctggcaa tagcgttgag caagagggca ttttccttaa
    22261 cagtaggagc aaaaggtcca atctggtcta atgagctacc aaaggcaatg agaccgaaac
    22321 gtgaaactgt tccgtaggtt ggtttgagac caacgattcc gttgaaggca gcaggttggc
    22381 ggatggaacc accagtatca gaaccaagtg acaagcgaac ttgtcctgag gctacagctg
    22441 cggcagaacc acttgatgac ccaccaggaa ccttgctgtg gttccaagcg tttttagttg
    22501 ctccgtagtg tgaagtttca cctgaaccac ccatagcaaa ttcgtccatg ttggtctttc
    22561 caacgacaat catgcccttg gtttttgcat tggcaacagc tgtcgcatca aagattggct
    22621 catagttgta gagcattttt gaggcagcag ttgtgagaat accgtctgta gagatgttat
    22681 ccttaacagc aagtggaatt cctgaaagga cattgtcagc atcaattcca gcttcatcaa
    22741 tggctttagc ttgaacaaga gcttgctcct cagcgatggt gacaaatgaa ttgagggctt
    22801 cctcacgaga cttgatattt tcaagtgttg cttgggtcaa ttctgttgca gaaatttcct
    22861 tagagacaag gagattgtgc aactcttcaa tagttttatt gttaaaagtc attaggcatc
    22921 tcctccattg tctaggatag ctggcacctt gatatagtag ttgtcttttt caggtacgtt
    22981 tttaaacaag cgatcacggt ctattccttc ttcggccaca tcagggcgga gtacagtctt
    23041 gcggtcagcc atagtcgtag taggtgcgac accagttgtg tcaacttcgc ccagcaattc
    23101 aaccatgtca acaatcttag acaaggtggt cgcaaaggca gcagtttctt cttcagagaa
    23161 tcttaatttt gaaagattgg caacgtgtgt tacctcttct tgcgtaattt tcatcttgca
    23221 tcctttcgtg aaatgatgat ttttagtctg ttctatttta ccatattttc ctataaataa
    23281 gggaggggga agtgaaaaga aatagaaatt gaaaaaaatg ctaaaatccg atataatgga
    23341 gtgttgaaag gaatggtaaa atccaatgta aaaatcattc tcagctattg aaacaagaaa
    23401 aatagagaat caaagaaaga gaacttatga atattcaaga agaaattaag aaacgtcgta
    23461 cctttgccat tatctcccac ccggacgcgg ggaaaacaac catcactgag cagttactct
    23521 actttggggg tgagattcgt gaggctggta cggtaaaagg gaagaaaaca gggacttttg
    23581 ctaaatctga ctggatggat atcgagaagc aacgtgggat ttctgttact tcatctgtta
    23641 tgcaatttga ctacgacggc aagcgcgtga atatcttaga cacgccaggg cacgaggact
    23701 tctcagaaga tacctatcgt accttgatgg cggtggatgc tgcggtcatg gtcgtggact
    23761 ctgccaaggg gatcgaggct caaaccaaga aattgtttga ggttgtgaaa catcgtggca
    23821 ttccagtctt tacctttatg aacaagctgg accgtgacgg tcgtgagcct ttggatctct
    23881 tgcaagaatt ggaagaaatc ttgggcattg ctagctaccc tatgaactgg cctatcggga
    23941 tggggaaagc ctttgagggc ttgtatgacc tctataacca acgtttagag ctttacaaag
    24001 gggatgagcg ttttgccagt ctagaagacg gggacaaact ctttggtagc aatcctttct
    24061 acgagcaagt caaggatgac attgagcttt taaatgaagc cgggaatgag ttttcagagg
    24121 aagctattct ggctggagaa ttgacgcctg tctttttcgg ttcagccctg acaaactttg
    24181 gtgtgcagac cttccttgaa accttcctca agtttgctcc agaaccacat ggtcacaaga
    24241 aaacagacgg tgaaattgtg gatccttatg acaaggattt ctcaggcttt gtctttaaaa
    24301 tccaagccaa catggaccct cgtcaccgtg accgtattgc ctttgtccgt atcgtatctg
    24361 gcgaatttga gcgcggcatg agtgtcaatc tccctcgtac tggtaagggg gctaaactat
    24421 ctaatgttac ccagtttatg gcggagagtc gtgagaatgt gaccaatgcc gtggcaggtg
    24481 atattatcgg ggtttacgat accggtactt atcaggttgg ggataccttg acggttggaa
    24541 aaaacaagtt tgaatttgaa cccctgccaa cctttacacc tgagattttc atgaaagttt
    24601 ctgctaagaa tgttatgaag caaaaatcct tccacaaggg gattgagcaa ttggtgcaag
    24661 aaggagccgt tcagctttat aagaattacc aaacaggtga gtacatgcta ggcgctgtag
    24721 gtcaactcca gtttgaagtc tttaaacacc gcatggaagg cgagtacaat gcggaagtag
    24781 ttatgaaccc aatgggtaaa aagaccgttc gttggatcaa gcctgaggac ttggatgaac
    24841 ggatgtcgtc aagtcgcaat atcttagcca aagaccgttt tgaccaacca gtctttctct
    24901 ttgaaaatga ctttgccctc cgctggtttg cggacaagta tccagacgta gagttagagg
    24961 agaagatgtg actcagtacc aacaattgga actaaagttc caattgttgg acgctagtcg
    25021 ctatttggcg aactagctaa ctgcctcact aactacgttt ggcaaataga atcgatttgc
    25081 caaactccgt gtcgtaatct aataatcggt aaccgctatg gcggcttacc taaacgcttc
    25141 actaggaaaa atccacgaat ggtattgatt tgtggatttt tccgtcgtag cagaaagtag
    25201 gtagcctagc taactgcctt actaactacg tttggcaaat gaaatcgatt tgtaaaactc
    25261 cgtgtcgtaa tgtaaaaatt gtaaaccgta atgggacttc atctaaaatc tccactggta
    25321 tggttttacg aacgaatagg cgaaaataat ggttacgtag aaacatatga tgggaataaa
    25381 aagaaatgag ataaaaagtg aaagaagaga aaaaagcgat tgtcttaggt gcagataatg
    25441 cttatatgga taaagttgaa acaacaataa aatctctttg tgttcatcta ttataatctt
    25501 aagttttatg tttttaatga tgaccttccg cgagaatggt tccaattgat ggaaaaaaga
    25561 ctagagactt tgaatagtga gattgtgaat gtttagattg acagtagtat tctgaaaggt
    25621 tatcgtctcc cctttgaggg actttcatat gcggcttttt tagatatttt attccaaagt
    25681 atgtaagcga gtctcgtgta ctgtatttgg atttagatat tgttgtaaga aaatcgatag
    25741 atgagctgtg ggatttggac ttgacggcta tacccttagc tgcagttaga gatgattttt
    25801 atacacataa ttttaattct ggtgttctgt taatcaacaa tggcatgtgg agagcagaaa
    25861 atgtcacgca agacctgatt taagacacaa ataaaaaatc ttccaattcc cttgcaaaaa
    25921 acaagaaagt gtgataacat agtacggtat gtggtgctag cacatccgct atattagatc
    25981 taataggagg aaaacacaat ggctaaagta tgttacttta caggtcgtaa gactgtatca
    26041 ggaaacaacc gttcacacgc gatgaaccaa acaaaacgtg ccgtaaaacc aaaccttcaa
    26101 aaagttactg ttcttatcga tggtaaacct aaaaaagttt gggcttcagc tcgtgctttg
    26161 aaatcaggta aagttgaacg cgtttaataa taaaagcaaa ggagacctta gggtcttttt
    26221 tcttttattg tgggtataac atcgtttgaa aaatagagta aatatccgct gatacatcat
    26281 tctgctttta cacttgggct gaaatatgat aaaatagagt atcaactagt tgaggtaaaa
    26341 aggatgactg taaaaattaa tacaaaagat ggtcaaatcg aactaacaga tgaagtgatt
    26401 gcaaccgtcg taggtggagc agcaactgag atttttggtg tggtcggtat ggctagtaaa
    26461 aatgccctca aagataattt ccaagccctg ctaggtaagg aaaattattc taaaggtgtc
    26521 gtcgtaaagg cagccgaaga tggcagtatt gcagttgatg tatataccgt gttaagctac
    26581 ggaacaaaga ttagtgaagt gtcaaaaaac attcaagagc gtgttgcctt tagcttggaa
    26641 aaccagcttg gaattactgc tcagactgta aatgtctaca ttcaaaatat caaagttgta
    26701 ggagaataat cgtgtcaaaa attactacta gcttatttca agaaatggtg caggctgcat
    26761 caactcgctt gaataagcaa gctgaatatg tcaattcatt aaacgtcttt ccagttccag
    26821 atggagatac tgggacaaat atgggaatga ccattgaaaa tggtgctaaa gaagttgcag
    26881 acaagccagc ttctacagtt ggagaagtag cgagcattct tgccaaaggg cttttgatgg
    26941 gtgcgcgtgg gaactcagga gtgattacgt ctcagctttt ccgtggattt tcccaagcta
    27001 tcaaggataa agacgagtta acaggtcaag acttggctct ggccttccaa tcaggtgtgg
    27061 aagttgccta taaggcagtg atgaaacctg ttgaaggaac gattttaaca gtttctcgtg
    27121 gagctgctat cggtgctaag aaaaaagctg agcaaacaga tgacgctgtt gaagtcatgc
    27181 gcgcagcctt ggaaggtgct aaaacagctc tagctaaaac accagacatg cttccagtat
    27241 tgaaagaagt tggcgttgtg gactcaggtg gtcaaggatt ggtcttcatc tacgaaggtt
    27301 tcctttcagc ccttactggc gaatatattg catctgagga ctttgtagcg actcctgcca
    27361 acatgagtga gatgatcaat gtagagcatc ataagtctgt agctggtcac gtagcgactg
    27421 aggacatcac gtttggttac tgtactgaaa tcatggtagc tcttaagcaa ggtccaacct
    27481 atgctaaaga ttttgactac gatgaattcc gtaactactt ggatgagctt ggagattctc
    27541 tcctcgttgt taacgatgat gaaattgtca aagtccatgt tcatacagaa gatccaggac
    27601 ttgttatgca agaaggtctc aaatatggta gcttggtcaa ggtaaaagtt gacaatatgc
    27661 gtaaccaaca cgaagcacag gttgagaaag aagctactca agttatcaag tcggctgaag
    27721 aaaaagagta tgctttgatt gctgtggtgg ctggtaaagg tctagcagat atcttctgtt
    27781 ctcaaggcgt ggattatgtt atcgaaggcg gtcaaaccat gaacccttca acagaagact
    27841 ttatcaaggc tgttgaacag gttaatgccc gtaacatcat cttcttgcca aacaacaaga
    27901 acatcttcat ggcagctcaa tctgcggcag aagttttgga gcaaccagcg gtagtggtag
    27961 aggctcgcac tcttcctcaa ggtatgacaa gtcttcttgc ctttgatcca agcaagtcca
    28021 ttgaagaaaa ccaagagcgt atgacagctg ctcttagcga tgtcgttagc ggaagcgtca
    28081 caacagccgt gcgtgataca acgatcgatg gcttagaaat ccatgaaaac gataatttag
    28141 gtatggtgga tggaaaaatt cttgtgtcaa accctgatat gcaccaaaca ttgactgaaa
    28201 ccttgaaaca tatgttggat gaagacagtg aaatcgtaac cttctatgta ggtgaagacg
    28261 gaagcgaaga acttgccaat gaaatcgctc aagaaatcgt agaagaattc gaagacgttg
    28321 aagtcgagat tcaccaaggt caacaacctg tttacccata cctatttagt gtggaataag
    28381 attaatgaag gactgagaaa tcagttcttt tttcttttta gtaaatgaaa tcggtatctt
    28441 tttaataaaa acaaaaataa cattcataaa taaacgttaa aatagaaaat tcagaaaatt
    28501 tcttctttta tcttgaaaaa ttttgaaaaa atggtatgat agtaacaagt tatttttaag
    28561 aggaaagaaa ggggaataat ggagaaaatc agtttagaat ctcctaagac ggggtcggac
    28621 ctagttttgg aaacacttcg tgatttagga gttgatacca tctttggtta tcctggtggt
    28681 gcggttttgc ctttttatga tgcgatatat aattttaaag gcattcgcca cattctaggg
    28741 cgccatgagc aaggttgttt gcatgaagct gaaggttatg ccaaatcaac tggaaagttg
    28801 ggtgttgccg tcgtcactag tggaccagga gcaacaaatg ccattacagg gattgcggat
    28861 gccatgagcg atagcgttcc ccttttggtc tttacaggtc aggtggcgcg agcagggatt
    28921 gggaaggatg cctttcagga ggcagacatc gtgggaatta ccatgccaat cactaagtac
    28981 aattaccaag ttcgtgagac agctgatatt ccgcgtatca ttacggaagc tgtccatatc
    29041 gcaactacag gccgtccagg gccagttgta attgacctac caaaagacat atctgcttta
    29101 gaaacagact tcatttattc accagaagtg aatttaccaa gttatcagcc gactcttgag
    29161 ccgaatgata tgcaaatcaa gaaaatcttg aagcaattgt ccaaggctaa aaagccagtc
    29221 ttgttagctg gtggtggaat tagttatgct gaggctgcta cggaactaaa tgaatttgca
    29281 gaacgctatc aaattccagt ggtaaccagt cttttgggac aaggaacgat tgcaacgagt
    29341 cacccactct ttcttggaat gggaggcatg cacgggtcat tcgcagcaaa tattgctatg
    29401 acggaagcgg actttatgat tagtattggt tctcgtttcg atgaccgttt gacggggaat
    29461 cctaagactt tcgctaagaa tgctaaggtt gcccacattg atattgaccc agctgagatt
    29521 ggcaagatta tcagtgcaga cattcctgta gttggagatg ctaagaaggc cttgcaaatg
    29581 ttgctagcag aaccaacagt tcacaacaac actgaaaagt ggattgagaa agtcactaaa
    29641 gacaagaatc gtgttcgttc ttatgataag aaagagcgtg tggttcaacc gcaagcagtt
    29701 attgaacgaa ttggtgaatt gacgaatgga gatgccattg tggtaacaga cgttggtcaa
    29761 caccaaatgt ggacagctca gtattatccc taccaaaatg aacgtcagtt agtgacttca
    29821 ggtggtttgg gaacaatggg ctttggaatt ccagcagcaa tcggtgctaa aattgctaac
    29881 ccagataagg aagtagtctt gtttgttggg gatggtggtt tccaaatgac caaccaggag
    29941 ttggctattt tgaatattta caaggtgcca atcaaggtgg ttatgctgaa caatcattca
    30001 cttggaatgg ttcgccagtg gcaggaatcc ttctatgaag gcagaacatc agagtcggtc
    30061 tttgataccc ttcctgattt ccaattgatg gcgcaggctt atggtattaa aaactataag
    30121 tttgacaatc ctgagacctt ggctcaagac cttgaagtca tcactgagga tgttcctatg
    30181 ctaattgagg tagatatttc tcgtaaggaa caggtgttac caatggtacc ggctggtaag
    30241 agtaatcatg agatgttggg ggtgcagttc catgcgtaga atgttaacag caaaactaca
    30301 aaatcgttca ggagtcctca atcgctttac aggtgtccta tctcgtcgtc aggttaatat
    30361 tgaaagcatc tctgttggag caacagaaga tccgaatgta tcgcgtatca ctattattat
    30421 tgatgttgct tctcatgatg aagtggagca aatcatcaaa cagctcaatc gtcagattga
    30481 tgtgattcgc attcgagata ttacagacaa gcctcatttg gagcgcgagg tgattttggt
    30541 taagatgtca gcgccagctg agaagagagc tgagatttta gcgattattc aacctttccg
    30601 tgcaacagta gtagacgtag cgccaagctc gattaccatt cagatgacgg gaaatgcaga
    30661 aaagagcgaa gccctattgc gagtcattcg cccatacggt attcgcaata ttgctcgaac
    30721 gggtgcaact ggatttaccc gcgattaaaa atccaactta aatttattaa accagcctaa
    30781 aaggcaataa ataatagaaa agagagaaaa gctatgacag ttcaaatgga atatgaaaaa
    30841 gatgttaaag tagcagcact tgacggtaaa aaaatcgccg ttatcggtta tggttcacaa
    30901 gggcatgcgc atgctcaaaa cttgcgtgat tcaggtcgtg acgttattat cggtgtacgt
    30961 ccaggtaaat cttttgataa agcaaaagaa gatggatttg atacttacac agtagcagaa
    31021 gctactaagt tggctgatgt tattatgatc ttggcgccag acgaaattca acaagaattg
    31081 tacgaagcag aaatcgctcc aaacttggaa gctggaaacg cagttggatt tgcccatggt
    31141 ttcaacatcc actttgaatt tatcaaagtt cctgcggatg tagatgtctt catgtgtgct
    31201 cctaaaggac caggacactt ggtacgtcgt acttacgaag aaggatttgg tgttccagct
    31261 ctttatgcag tataccaaga tgcaacagga aatgctaaaa acattgctat ggactggtgt
    31321 aaaggtgttg gagcggctcg tgtaggtctt cttgaaacaa cttacaaaga agaaactgaa
    31381 gaagatttgt ttggtgaaca agctgtactt tgtggtggtt tgactgccct tatcgaagca
    31441 ggtttcgaag tcttgacaga agcaggttac gctccagaat tggcttactt tgaagttctt
    31501 cacgaaatga aattgatcgt tgacttgatc tacgaaggtg gattcaagaa aatgcgtcaa
    31561 tctatttcaa acactgctga atacggtgac tatgtatcag gtccacgtgt aatcactgaa
    31621 caagttaaag aaaatatgaa ggctgtcttg gcagacatcc aaaatggtaa atttgcaaat
    31681 gactttgtaa atgactataa agctggacgt ccaaaattga ctgcttaccg tgaacaagca
    31741 gctaaccttg aaattgaaaa agttggtgca gaattgcgta aagcaatgcc attcgttggt
    31801 aaaaacgacg atgatgcatt caaaatctat aactaattag aaatatatag cgctggagat
    31861 gattttatga aaaagattat gagaaaaatt gcatcgttat tattggttct agttgtataa
    31921 tgtaattaca ccgtcggtaa tagtgctagc agaccaaaat aaagcagatt ggtcgtatga
    31981 tgaaaatgct gtaattaaca tttatgatga tgctaatttt gaagatggta ggttgcatat
    32041 gacctttgaa caattcttca aattggcaca aatagctaga gaagaaggtc ttgaaattca
    32101 ttctccgttt gagagagctg gtgcgactaa atctgctcgt tatatagcga aatggatttt
    32161 gagaaataaa aaatattaac aaatatagtt ggtaaatcat taggacctaa atcagctgtt
    32221 agattcggag aagctttatc ctatattgaa ggtcctcttc gcagaataaa tgagacgata
    32281 gatggcggtt tatatcaaat agagcaaatt attgcatctg gattgaaaga atcgggttta
    32341 aatgactgga ctgcgaaaac tttagcttca gctattcgtg ggatattaga tgtacttatt
    32401 taggggttga aatcatatga atattaccaa tttgttttct atcaagacag gatgtgatga
    32461 aactgatagg caactgcaaa aactattttt tcagttggat ttacaattgg gagaattgac
    32521 agatcaacta agaaaattag attctaattt tgttcctcgt agtcaatttg tagacacgtt
    32581 ggattt
//
LOCUS       DAVHIT010000017        31935 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVHIT010000017 DAVHIT010000000
VERSION     DAVHIT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.017"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(137..1699)
                     /gene="guaA"
                     /locus_tag="VVN16_000641"
     CDS             complement(137..1699)
                     /gene="guaA"
                     /locus_tag="VVN16_000641"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5572082.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1841..2539
                     /locus_tag="VVN16_000642"
     CDS             1841..2539
                     /locus_tag="VVN16_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5572083.1"
                     /translation="MLPAYMKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPERFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2582..3478)
                     /locus_tag="VVN16_000643"
     CDS             complement(2582..3478)
                     /locus_tag="VVN16_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5572084.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFTIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3487..4422)
                     /locus_tag="VVN16_000644"
     CDS             complement(3487..4422)
                     /locus_tag="VVN16_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU5572085.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4419..5144)
                     /locus_tag="VVN16_000645"
     CDS             complement(4419..5144)
                     /locus_tag="VVN16_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU5572086.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5228..5863)
                     /locus_tag="VVN16_000646"
     CDS             complement(5228..5863)
                     /locus_tag="VVN16_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU5572087.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     ETLNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5865..6692)
                     /locus_tag="VVN16_000647"
     CDS             complement(5865..6692)
                     /locus_tag="VVN16_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5572088.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6714..7049)
                     /locus_tag="VVN16_000648"
     CDS             complement(6714..7049)
                     /locus_tag="VVN16_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEU5572089.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7050..7766)
                     /locus_tag="VVN16_000649"
     CDS             complement(7050..7766)
                     /locus_tag="VVN16_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU5572090.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNGIKAALLAYSYGFNGIEQYISKEDYNRYLSD
                     LNEDKMKAEIERAEKEADITIIMLQMGVEYQLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            8279..8890
                     /locus_tag="VVN16_000650"
     CDS             8279..8890
                     /locus_tag="VVN16_000650"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5572091.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            8935..9663
                     /gene="rlmB"
                     /locus_tag="VVN16_000651"
     CDS             8935..9663
                     /gene="rlmB"
                     /locus_tag="VVN16_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU5572092.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9701..10612)
                     /gene="trxB"
                     /locus_tag="VVN16_000652"
     CDS             complement(9701..10612)
                     /gene="trxB"
                     /locus_tag="VVN16_000652"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU5572093.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQVFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10678..10902)
                     /locus_tag="VVN16_000653"
     CDS             complement(10678..10902)
                     /locus_tag="VVN16_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU5572094.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(10980..11723)
                     /locus_tag="VVN16_000654"
     CDS             complement(10980..11723)
                     /locus_tag="VVN16_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5572095.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11723..12523)
                     /locus_tag="VVN16_000655"
     CDS             complement(11723..12523)
                     /locus_tag="VVN16_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5572096.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12681..13037)
                     /locus_tag="VVN16_000656"
     CDS             complement(12681..13037)
                     /locus_tag="VVN16_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU5572097.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13031..13561)
                     /locus_tag="VVN16_000657"
     CDS             complement(13031..13561)
                     /locus_tag="VVN16_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5572098.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(13561..14055)
                     /locus_tag="VVN16_000658"
     CDS             complement(13561..14055)
                     /locus_tag="VVN16_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572099.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14190..14636
                     /locus_tag="VVN16_000659"
     CDS             14190..14636
                     /locus_tag="VVN16_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU5572100.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14633..15280
                     /locus_tag="VVN16_000660"
     CDS             14633..15280
                     /locus_tag="VVN16_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU5572101.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15301..15882)
                     /gene="pdxT"
                     /locus_tag="VVN16_000661"
     CDS             complement(15301..15882)
                     /gene="pdxT"
                     /locus_tag="VVN16_000661"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU5572102.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15883..16758)
                     /gene="pdxS"
                     /locus_tag="VVN16_000662"
     CDS             complement(15883..16758)
                     /gene="pdxS"
                     /locus_tag="VVN16_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5572103.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16910..18289)
                     /locus_tag="VVN16_000663"
     CDS             complement(16910..18289)
                     /locus_tag="VVN16_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5572104.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18583..19506
                     /locus_tag="VVN16_000664"
     CDS             18583..19506
                     /locus_tag="VVN16_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU5572105.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19566..20171
                     /locus_tag="VVN16_000665"
     CDS             19566..20171
                     /locus_tag="VVN16_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5572106.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20190..21434
                     /locus_tag="VVN16_000666"
     CDS             20190..21434
                     /locus_tag="VVN16_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5572107.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGSHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21551..21808)
                     /locus_tag="VVN16_000667"
     CDS             complement(21551..21808)
                     /locus_tag="VVN16_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU5572108.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(21850..23886)
                     /locus_tag="VVN16_000668"
     CDS             complement(21850..23886)
                     /locus_tag="VVN16_000668"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU5572109.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVHIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24146..25063)
                     /gene="glyQ"
                     /locus_tag="VVN16_000669"
     CDS             complement(24146..25063)
                     /gene="glyQ"
                     /locus_tag="VVN16_000669"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU5572110.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(25256..26017)
                     /locus_tag="VVN16_000670"
     CDS             complement(25256..26017)
                     /locus_tag="VVN16_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5572111.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26290..27132)
                     /locus_tag="VVN16_000671"
     CDS             complement(26290..27132)
                     /locus_tag="VVN16_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5572112.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEKSVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27246..28637)
                     /locus_tag="VVN16_000672"
     CDS             complement(27246..28637)
                     /locus_tag="VVN16_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5572113.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(28872..29249)
                     /locus_tag="VVN16_000673"
     CDS             complement(28872..29249)
                     /locus_tag="VVN16_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU5572114.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            29464..30441
                     /locus_tag="VVN16_000674"
     CDS             29464..30441
                     /locus_tag="VVN16_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5572115.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30438..31520
                     /locus_tag="VVN16_000675"
     CDS             30438..31520
                     /locus_tag="VVN16_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5572116.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     TEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31584..>31935)
                     /locus_tag="VVN16_000676"
     CDS             complement(31584..>31935)
                     /locus_tag="VVN16_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5572117.1"
                     /translation="AGGCNTEWMNAFLEELSQAYPDDYLLLVMDNAIWHKSSTLKIPT
                     NIGFAFIPPYTPEINPVEQVWKEIRKRGFKNKAFRTLEDVMNQLQDIIQELEKEVIKS
                     IVNRRWIRMLFENR"
BASE COUNT         9458 a         7169 c         5727 g         9581 t
ORIGIN      
        1 ttttttgtcc aaatgtaaac cacttggaac tattattcaa tttattcttt ttagtaaatg
       61 ttcccgtttt aatcccagtt acagttttta atcctctgta accgttgatt ttacaaagct
      121 tttaattttt ttgcgattac tcccactcaa ctgttgcagg tggtttactt gtaatatcgt
      181 agacgatacg gttaacatga tccacttcat ttacgatacg tactgagatt ttttgaagta
      241 cttcccatgg aattttggca aaatcagcag tcataccatc gatagaagtg atagcacgga
      301 ttgcaatcgt gtagtcatac gtacgaccgt cacccataac accgactgaa cgaacgcctg
      361 tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc agctttagcg atttcttcac
      421 gaagaatagc gtctgattca cgaacggttt caagtttctc ttcagtgatt tcacccatga
      481 cacggatagc aagtcctggt cctgggaatg gttggcgcca tacgatatgg tctggcatac
      541 caagctctgt accaagagca cgaacttcat ccttgtaaag agtattgagt ggttcaatca
      601 attcaaactg catatcttct ggaagaccac ccacgttgtg gtgtgacttg atagtttgag
      661 ctgtatccgt accagactcg ataacatctg tatataaagt accttgagca aggaatttca
      721 catctttgag cttgcttgct tcgtcatcga atacatagac aaactcgtta ccgatgattt
      781 tacgtttttg ttcagggtca gaaacgccag caagtttgtc aaggaaacgt ttagcagcgt
      841 ctgctttgac gatattcaaa ccaaacttac caccgagcat gtccataact tgatcagctt
      901 cgcctttacg aagaagaccg tggtctacga agatacagat caattgatcg ccaatcgctt
      961 tttggagaag aaccccaacg acagatgagt caacaccacc tgatagacca agaaggacac
     1021 gtttatcacc gacggtttca cgaatttttt tgatctgcat gtcaatgaaa ttatccattg
     1081 accagtcacc tttagcctta caaatgttaa gggcaaagtt acgaaggata tcatttccgt
     1141 atacagaatg acgaacttct gggtggaatt ggataccgta aatgtgttta tctgggtttt
     1201 cgatggctgc gtatgggcag tcagctgatg tacctgtacg aacaaagtca gcaggaatct
     1261 cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc atcaggtgtt gattcaaaaa
     1321 gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc acgatttcca gcatcacctg
     1381 caggaacaac ttttcctcca agtttatggg tcaataactg cataccataa cagattccca
     1441 aaattggaat tccgagttcg aagatttctg ggtcaatatc aaatgaacca tcttcatata
     1501 cagaatttgg accacctgat agaataattc ctacaggatt gacttcacga acttcagcag
     1561 ctgaaatttt atggcttttt agttctgaaa aaacaccaat ctcacggata cggcgtgaaa
     1621 tcagctggtt gtactggcta ccatagtcca atacgatgat tttttctaca tcttgcaaat
     1681 cagttgaaat gttgctcatc tttttccttt ctcaaaagaa atatcttttc ccaatattct
     1741 atcacaaata agggcgaatt accaactaaa caaggcaaag tttgactttt tctagtttat
     1801 tggggtacaa tagagaaaag atagaaatga agtgaaaaat atgctacctg cttatatgaa
     1861 aatccatgat cagataaaaa aggatattga cgagtaccgt tgggctattg gtgagaggct
     1921 tccaagtgaa agagatttag ctgagcagtt tgcggtcagt cgtatgaccc tccgccaagc
     1981 cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt gtaggaagcg gcacctttgt
     2041 ttccagtact cgagtacaag aaaagatgcg agggacaacc agttttactg aaattgtcaa
     2101 atcccaaggt aaagttccct ctagccagct catttcctac aaaaaaacca ttcccaatga
     2161 gcaagaagtt gccaagctag gaatttttcc aacggacaat attatccgaa tggagcgggt
     2221 ccgctatgcc gaccaagttc ccctagttta tgaagttgct tctattcctg aaagattcat
     2281 taaggacttt aaaaaagaag aaatcaccag tcatttcttc caaaccttgc aaaaacatgg
     2341 ttatcgtatc ggcaaatctc aacagaccat ctatgctcgc cttgctaaag aaaagattgc
     2401 ccactatttg gaagttgaaa aaggacatgc tattcttgga ttgacccagg tttcctacct
     2461 agaagatggt actgcttttg aatacgtaaa aagtcaatat gtaggcgaac gttttgaatt
     2521 ttatcttgaa aataattaga ttaccacgca aaaactctct gtcacggaca aagagtcttt
     2581 tttatttttc taagaataaa tctttcaaag aaatcaaagt tacagcaaac aatatcattg
     2641 ccataccaag aaaatcaatg ggataaaatt gttcattcat tattaagaag gcaaagaaaa
     2701 ccgccgatat tggctcaatt gaagccaaca agcttgactt gaccggtcct atcagactgg
     2761 ctcctttaag gaaagctgta taggcaaaga cagtcccgat aaggataatg cctgcaaacg
     2821 caaggagaaa atcaagacta gtcgggatat cggcctgtag aacccctgta aaaggaaggg
     2881 cgaccaaacc tgctatgacc attcccacac caatgaccaa gctgctcccc cactttttaa
     2941 tcaaggctat gggtaaaatg atatacagag cataagtcaa ggcagaaaag agaccccaga
     3001 acagaccagc aggtgtcatg gataactggt ccaactgccc atgtgttgcg atcaggaagg
     3061 ttcctccgat ggtgaatatg atggaaacta tctctcccag tgtcggtgcc accctatcct
     3121 tgatacagct ataaattaaa attccgacag gacaaacata ctgaagcacc gtcgctgttc
     3181 ccgcattggt ctcctgaata gcagacagat aggcgaattg gttgagaaaa agaccaatca
     3241 gagcaaaaat aagaagagac agcaaactct ttctatcctt taaaaagacc agtattttat
     3301 cctttgcagt agcataagcc aagagcatga gaattccacc agcgattaaa agacgcaagt
     3361 tggttaagac cagagccgaa attccgtgtg ccatcaggta ttggccactc gttcctgaca
     3421 acccccaagc aataccagcc acaactgtta gtaaagtccc ttttaaacta tttgacatgt
     3481 ctctccttac tcttctttgc gaatcaagtt catcacttga ttgcggtcta atatccattg
     3541 agctctctcg tccttttcat aggtacgatt cgataggaaa atagtggctt cttgcttctg
     3601 acgattccac atgataaagg tacctgtata gcccgtatgg tctagccaat ctccttccaa
     3661 attccatgct aaagaacgtt ccttgtcatc caaaggagaa aaattttgac ttaagtctct
     3721 tgcaaaatca tctgctaaat agtgttctaa aaagatttgt aaatccttta tagtcgaaaa
     3781 taaaccagcg ctcccagcat gtctacccag gagacgagcc ttgggatcat gcactatgcc
     3841 tgcctctaca cctctaactg ttggaacagc aagctcaact ggcccaaact tggtttccgt
     3901 cattccccaa ggtttccaga cttgatcctt taaaatcaca tccaaatctt gattaaaaat
     3961 tctttccaaa ataaagccca acagcaaaaa atggacatcc gaataaagaa aggctggctg
     4021 acttcgtctg ttgagatgaa acatcgcttc ctttaattca ggggctgtta aaagatcacg
     4081 attaggaata aaaggatcaa ggtctgttgc atgagtcaag agctggcgaa tagtaatgtc
     4141 tggataatca ctctcaggta aaaaatctat taccagtcta tcaatatcta attgacctat
     4201 ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc ttgctgacac tagctaggtc
     4261 ataaactagt cctgcctcag tctgcaagcc atgctctggg tcactctggc ctaaatagaa
     4321 ctctgtccat tgattgtcct taaaatacgc aaaagaggct ccgggataaa ttcctgcctc
     4381 gatttgttct tctatttttt taataatctt ggtccacttc atacttcttc aaaccacaat
     4441 tcaacattat ttctatcttt accaaggaag aatttttcag acttaggaat aaaatattca
     4501 gtagactcaa acttctggcg aagacttgct atgtctaatt cattgaccaa gaacttgagc
     4561 atagataagt cccaggtaac cgtattgtcc acagtcaaat cctgcccctg agctggaata
     4621 aaatcaaggg atgccccaat ttcagatgat tccaagaaac tttcgatatc agttgggaga
     4681 tgtaattcca tagaaatctc aaatttactt aaagaaattg atgccaaatc tgtttgaaat
     4741 tcaggctttt ctcctacttc tactagactt gctctgtcat cttccgcatg aatcaaaatc
     4801 aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat tttgaccttt atataatcga
     4861 tgaatcgaat ctgttttaga taagattcct tcaatttcta agggattttc caccttgaca
     4921 atcaatctag ctagtttttt tcttccctct accttacgag tacgcatact gggagcttct
     4981 tctaaaacca gcttttcaag acccgtttgg tcacctagtg acagaaaggc cgattcttct
     5041 aacaaggcct tcattccaag ggtttcaata taaaatgttt catttaattt tctattatta
     5101 gcttttaaag taggaataat ccgtacaatc tgatttacat tcataaattc ctccgtcact
     5161 ttttatttta aaggatttta gaatttttta caagatatta tgctcaaatt taacattatt
     5221 tagctattta gtaaagatag tctttcaagg cttttgacaa agcctgataa ccagcaatac
     5281 tgaggtgcag tccatcagtt gtatattctt ttttgagttg gcctgcttgg tctgtcaaac
     5341 aatcaaatac tggcacaaat tccacctgca tataggcaga tgcaagctct tgataggctt
     5401 gattccaatt ctgaattttt tcattcgagc ggatatagac tgcctgctgg tactcctctc
     5461 cctcattgac aggcaaaatg gaaagcaatt taatctctgt caatggataa tcgcgagcaa
     5521 cggattgaat gatagcttcg agattattga gagtctcatt cacaggaaca tcctttccga
     5581 tatcatttgt cccaatcaga agaaaaattt tatctactgc tccaccatat agatgagcat
     5641 caaggttctc tagtaacagt cctgtctgat agccacgaat tcctcgattg acaatcatct
     5701 ttgaagttcc aaatagctcc tgtagaggat aatactcgac aatggaatcc ccaataaaaa
     5761 gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg atatttggtt tgaatttttt
     5821 cttgttcctt tagaagccaa ttttctaata actgtactgc cacttcattc tcctttttct
     5881 aaccagtttt ccaagacttg ataaactcct ccttggctgt tggctggtgc tagagcagtc
     5941 gccacagctt tggctttctc atcagcattt tccatcgcat aggcaattcc agccatttca
     6001 agcatttcaa catcattttc actatcacca aaagccatga tttcttggga tttcaagtcc
     6061 cagcgcttga gtaattcctc caagccccat gctttatgaa tcccagcttg gaggatatca
     6121 atgcaaccat agccactgga tacagctcgg acacggccat caaagagagc attgatttct
     6181 tccaaaaccg aactcaaacg ttcctcacca acaaccatgc tcatcttgag cacaccacca
     6241 aagaggtcag atgttaattc atccacaaat tgcatccgtt ggtagaattt ttcaatcatt
     6301 tctggagtca taaaactttc aagatctgta aaaatcgtac cttccttgac aaaatcaccc
     6361 ttcatccccg ttacaacaaa ctggtcctga cacgctcgac ccttgaaatg agccaaagcc
     6421 ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca attcattgtt ttcaaaaata
     6481 cgagcgccat tagcaacaac cagaaccact cgatccacca agggactcag tagttgtctc
     6541 atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa tgcccctttg atccaactga
     6601 tctaaaatct tttccaatcg tgggagatca agctgacctc tagcatccag caaggtccca
     6661 tccatatctg tcgcaattac cttaatcatc atttactttc ctctggattc aagctaatac
     6721 cacttcaatc ccacatgttc attcatttct ttataggctg tatcaattcg ttccttagtc
     6781 gcttcatcta actggtcacg atgactgcca ccctctataa aatcttccaa aatataagtt
     6841 tggtaatgat acaagggata tccttctggt gaaaaggttc cctttggtgt tcgattgacc
     6901 caagtaggat gagctttagc tgttccgata gttgtttttc catccttctt cttgatggtg
     6961 acatccatga gaacaccacg ttcagtccac ttagcattct cttcatctcc catagattca
     7021 atccgttgat tggaaatgaa gttccccatt taatagataa tgagcttctt atctccatct
     7081 ttttcaaccg tttcagatgg ttcaacaacg tgaggatgcc ctccaaagat aatatccgct
     7141 ccccaatcga tcatcttgtg ataaagagct ttttgttctt cagttggttc caattgatac
     7201 tcaacaccca tctgaagcat gataatggtg atatctgctt ccttctctgc ccgttcaatt
     7261 tcagccttca tcttatcttc gtttaaatct gaaagataac gattatagtc ttccttagaa
     7321 atatactgct caattccatt gaaaccatag gaataggcta acaatgcagc cttgatacca
     7381 ttcacttcct taatgaccag cggagcctga tcacgtggtt cgtgcgtata aactccgatt
     7441 ggagtgattc cagctttctc aataatatcg gccgttgaaa taactccctc aatttgcgaa
     7501 tccaaaatat gattatgagc taaatccagc acatgataac ctgcctcctt aatagcatcc
     7561 ataacttcaa caggagcatt aaagagaaga taacccgcta aataatgatc cttattaatg
     7621 gttccttcaa aatcaccaat agctaaatct gcttgcttga gccaaggagt cacatactca
     7681 aaattttcat gaaagtcata ggtaccgtct tcttttttag ctgaaaagaa aagtccatcg
     7741 tggtagagca aatccccatg ggccataatt ctggcagttt tttcctcttt ctactcctga
     7801 gacttttgct tagtcccctc ttgagaaata gtatctcgtt tttgactagt caagggattc
     7861 cttggaagct ttcaaacaac aagatcaagc ccattgataa accaactgct agcaagagta
     7921 tcgccacaaa tcccttattg ctccacttgc gataactcct aaaaagttta ccaagccctt
     7981 cataaaacga aaagctaaac caccctgatt tcgattttgt cttctttgta tcttcgttct
     8041 ccctactttc ttatgcaagc cttttctttt tattatatca cagataagta tttctttcac
     8101 aattgaattg aacttcccat ctattttcta taaatcctaa atgccataat gctttcaatt
     8161 cttgtcattt tgtgatatca tgtagaggaa atgaactaat ccacagtggc ttattccaag
     8221 tataccactt gggctttggc agtaactaac tgcactaaac ataatataag gagagaaaat
     8281 gtctgcaata gaacgtatta caaaagctgc tcacttaatt gatatgaacg atattatccg
     8341 tgaagggaat cctactctac gcgcgattgc tgaggaagtc actttccccc tatctgacca
     8401 ggaaatcatc ctaggcgaaa agatgatgca attccttaaa cattcccaag atcctgtcat
     8461 ggctgaaaaa atgggactcc gcggtggtgt tggactggct gctccccagt tagatatctc
     8521 aaaacgcatt atcgctgttt tggtacctaa tattgttgaa gaaggcgaaa ctccacagga
     8581 agcctacgat ttggaagcca ttatgtacaa tccaaaaatc gtctctcact ctgttcaaga
     8641 tgctgctctt ggcgaaggag aaggttgcct gtctgttgac cgtaacgtgc ctggctatgt
     8701 tgttcgccat gcccgcgtta ctgttgacta ctttgacaaa gatggagaaa aacaccgtat
     8761 caaactcaaa ggctacaact ccattgttgt tcagcatgaa attgaccaca ttaacggtat
     8821 catgttttac gatcgcatca atgaaaaaga cccatttgca gttaaagatg gtttactgat
     8881 tcttgaataa agaaaatccc gttgcaagac ggggttttgt gttataatag aggcatgaaa
     8941 acaaatgata ttgtctatgg cgtccacgcc gttaccgaag ccctccttgc aaatacagga
     9001 aacaaactct acctccaaga agatctccga ggtaagaatg ttgagaaagt caaggaacta
     9061 gctacagaaa agaaggtgtc catttcttgg acatcaaaaa aatctctctc tgagattact
     9121 gaaggtgctg ttcatcaagg ttttgttcta cgagtgtctg aatttgccta tagcgagcta
     9181 gattacatcc ttgcaaaaac acgccaagaa gaaaatccac ttctattgat tctagatggt
     9241 ctaaccgatc cccataatct aggttctatc ttgcgaacag ccgatgcgac caatgtttca
     9301 ggtgtcatca ttcccaagca ccgtgctgtc ggagtaactc ctgtcgttgc caaaacagcc
     9361 acaggtgcta ttgaacacgt tccaattgcc cgagtgacca acctcagtca aaccttagat
     9421 aaacttaagg atgaaggttt ctggaccttt ggaacggata tgaacggtac tccttgccac
     9481 aagtggaata caaaagggaa aatcgccctc atcattggaa atgaaggaaa aggtatctct
     9541 agcaacatca aaaaacaagt cgatgagatg attaccattc cgatgaatgg acatgttcaa
     9601 agccttaatg ccagtgttgc tgcggccatt ctcatgtacg aagttttccg aaatagacta
     9661 taaaaagttt ccagtcatct gattggaaac ttttttataa ttaactatgt tctgtaatga
     9721 atttataggc ttcttgacca gcgatagctc catctccaac cgctgttgtt acttggcgaa
     9781 ggtctttcaa gcgaacatct ccaactgcaa agataccgtc gactgcagtt ttcatgtggt
     9841 tatctgtcac aatccatcct gcctgatctt ggatattcaa ttctttaaca aaatcgctaa
     9901 gagggtccaa accaacatag ataaagacac caccgaagac ttgttctgtc acttgacctg
     9961 ttttcacatt ttcaaatacg actgattcta ctcggttttc acccttgatt tcccttacta
    10021 cagaatccca gataaagctg attttttcat tcgcaaaggc gcgatcttgt aaaacctttt
    10081 gggcacgaag ttggtcacga cggtgaacaa tggtaacagt cttagcaaaa cgagtcaaga
    10141 agagggcttc ttcaacagct gaatctccac caccaactac caataaatct tggtcacgga
    10201 agaaagcacc atcacacaca gcacagtaag aaacaccacg actgttcagt tcttcttctc
    10261 caggtactcc caaaggacgg tgtttagaac cagttgctac gataactgta cgtgtttcat
    10321 atgtttggtc atcagtcatc actttcttaa aatcaccatg gtcttcgaca ttttcaacat
    10381 aaccataaat gtgctccaca ccaagatttt caagtggttc aaacatcttt tcagccaatt
    10441 caggtccact aatattagcg tatcctgggt aattttcgat atcagatgta ttattcatct
    10501 gaccacctgg cagaccacct tcaatcaaag ctacttttag attgcttcga gcagcataca
    10561 aggccgcagt catccctgca ggtccagcac cgataataat agtatcgtac atatagattc
    10621 cttctttctt gttgtaacta tctttattct aactctttct tgtcaatcac gcaaggatta
    10681 tgccttgaaa acaagaatta aactcataac cgcaaaggat aatactggga caaccaagac
    10741 tccaatcata atcatatcat agagatgggc ttgacgagcc ctggctgtct tatcaactcc
    10801 cgacatggct ctcagaccaa tccaaatccc taaaaaaatc agaacaagga tggtggtcaa
    10861 gatcaaactc tcgaaatata aagaaaatag ttgcagtagc atgatttctc tcatttctat
    10921 cttttttaaa gagtaaactc agctagtcca actaactgag ttttccttta tctattatat
    10981 caaatataag tccgtttgta actagcgaag aattcttttg tccgctcttc tttaggggtg
    11041 tggataatct catccggagt tccagactcg atgattttcc ccttatctaa gaagagaatt
    11101 ttatccgcaa cttgggctac aaaggacatg tcatgactga ccaaaatcat ggtctgacct
    11161 gacttagcag catctgcaat agacttttct acttcaccga ccaattctgg gtcaagggct
    11221 gaagttggtt cgtctaagag caaaacatct ggtttcatag caagcgcacg cgctagggca
    11281 acccgttgct tctgtccacc tgataaatgg cgaggataat ggttttcacg gtccgaaagc
    11341 ccaaccttag ccaactcttc cttggcaatc ttagtcgctt cttggtcaga taatttcttg
    11401 acaacaacca agccttcttt cacattatca agtgctgttc ggcgttcaaa caaattaaac
    11461 tgttggaaaa ccatagacaa cttacggcgt agggcaagga tttcttcttg agtgatttta
    11521 gaaaaatcaa ctgaaaaacc atcaatctga atagagccac tgtcaggtgt ttcaagataa
    11581 ttgagactgc gaagaaaggt tgattttcca gctcctgaag aaccaatcaa ggctacgact
    11641 tctccttttt gaatatccaa gttcagatga tccaagacag tctgtcctga aaaggatttg
    11701 cttaaattcg aaatcttaat cattaacgaa ggtctccttt cacatctgtt tgcactgtat
    11761 caggtgcaga aatagccatt tttctctcga tgaaacgacc gaggctttca attccgatat
    11821 tgactaccca ataaacaagg gcaacggaga tgaagcgttc aaaatagcga taatcagctc
    11881 cacctagaat ctgagcttgg gcaaagactt ccacaacacc cgcactaaaa gctagagatg
    11941 ttcccttggt caaaccgatg agggaattaa tcaaggttgg agtagctacc accgctgcat
    12001 taggaataat cactcgtcga taaacttgcg ctcgggtcat acccagactg cgtgccgcct
    12061 caatctcacc aggattaact gagagaatgg ctgcacgaat ggtttcacta gcataagctg
    12121 cctcattaaa ggcaaaggcg acaatcgcaa aagctgcagc tggaatcgca ttgatattga
    12181 gaccagttcc ccattgctga ttgagggctt tcaaagccaa aggaattccg tagtaggtca
    12241 acatgagttg caccaaaatc ggtgtccctt ttaagaaact aacaaagaag gcctgcaagg
    12301 gatataaaat cttgacacga ttgatcttca caatggcaaa aagaagcgcc aaaaccaagc
    12361 caaaaagggc accgccaatt gtcaacataa ttgttgttgg aagttgttgg acaattctag
    12421 ggattccatc aaagaccgaa cgtaggctaa acagcttgcc atccggaatc aattgcatca
    12481 agttttggta ccaatctgat gctaaaatcg ttgtaacatt cataaaaaca tcctcttctg
    12541 ataatgattc attctaccat gcttctgccg tcaatgaaat tatttgctac ggtcagatac
    12601 agactttgtc tacctactag tttatcatac tttgaaacag ggaagttata tattttatct
    12661 atcaaagaga tagataaaaa ctatttcagt cccagttctt cataagattt tcgataaccg
    12721 atttgcttaa cagttccatt ttctactaaa atgggccgtt ttaacaacat accgtcactt
    12781 gctagcaact cagccgcttc ttggtttgac aaacttccta ccttatcttt tagccctaat
    12841 tcacggtatt tgataccact ggtgttgaaa aattgcttca attcaaatcc tgaggtttct
    12901 agccaattca aaatgacttc ttggctaggt gtttcttcca cgatatggac ggctttatag
    12961 tccacaccta attgatttaa ttcttgtttt gcttttttac aagttgaaca tttggggtat
    13021 tcgataaatt ctaacatgtg cttctccttc tattgtttct atctttaaaa tctactcctt
    13081 catgctccaa gagccaagct ttcttttcca ctcctgcagc ataacctgtc agacgcttgc
    13141 ctgctcccaa cacacgatga caaggtacta ggatagacca aggattgcgt cccactgctc
    13201 caccaattgc ttgagcagaa gccacttgca ggtcttgagc aatttgtcca taggtcactg
    13261 tctgaccata aggaatgccc tgtaaatagt cccaaactct cttttcaaaa ttcgttccga
    13321 ttggcgccaa gagcaagttg gataaatcct gaggcttgcc tttaaagtaa tcatctaagc
    13381 aagcaataac tgggtctaaa ataggatgac taacaacttc ttctatcgtt tcatctccta
    13441 gtcccctctc aaaatgcttc tgctcctgaa cccaaattcc atacaaataa tggtcatcag
    13501 ctacaagtga tagaatacca attggtgagg agtagaggat ttttacatac ttcttttgca
    13561 ttatttcttt aatttttgat aggccaagcg ttcgccatct actggcacct taccaacttg
    13621 tttaaaacca agtttttcaa aaatatgttg cataaccttg ttttcagcat gcgtatctga
    13681 gcgaaaatca agataatcaa aaccttcaat caagccctct aagaaggttt gagcaactcc
    13741 ttttccctgc acatctgctg ccacagcaat acggtgaaag actagatact ctgactctcc
    13801 cgcttgccag tttccctcat aaatagcttc ataggcctcc tctggactct tggtcacagc
    13861 agcataggct agcagttctc cctcttccaa ggctacgtag gcttgccctg agatgatatc
    13921 atcgataata acatctgcat ttggatagcc attttgccac tggtcactac cagcatctgc
    13981 taaacatttt ttggcatcct ccatcacctg catgatggca tctacttcat ttggaaaagc
    14041 taaacgaatt tccatctttt ttcctcattt ctgactagtt tctcccatca tagcataatt
    14101 taagtatttc tacaagcagt taaaacttga ctttgtgttt ttgttatttt ataatctagt
    14161 tatcaaaact atataaaaag gagttgaaaa tgaaaactac cttttcctac ccaaaatggg
    14221 cagaaattcc aaacattgac ctctatctgg accaggtttt gctctatgtc aatcaggtct
    14281 gcgcccctat ctctcctaat aaagacaagg gcctaacagc atctatggtc aacaattatg
    14341 tgaaaaatgg ttacctgaca aagcctgaca aaaaaaaata ccaacgccaa cagattgccc
    14401 gtttgattgc tatcacaacc ctcaagtctg tattttctat tcaagaaata gcccagacac
    14461 ttaatactct acaaactcaa gcaagttcag accaactcta cgatgctttt gtggactaca
    14521 tgaaccaagg aattgaccca gctaacccta ttatccaaac cagctgccaa accgttaaac
    14581 tctatcatca aactctagac ttaatcgatc atactcaaga ggaggtaatc caatgaacac
    14641 tagtcttaaa ctcagcaaac aactcagttt tggagaggag attgctaata gcgtgaccca
    14701 tgctgtgggt gcagtcatca tgcttatctt gctgcctatt tcatccatct atagttatga
    14761 agcacacgga tttttatcct ctatcggcgt ttccattttc gtcatcagtc tctttctcat
    14821 gttcctatca tccaccattt atcactctat ggcctatggt tcgacccaca aatatgtttt
    14881 gcgaatcatt gaccattcta tgatttacgt tgccattgcc ggctcataca cgcccgttgt
    14941 cttgaccttg atgaataact ggtttggcta tctgattatt gtcatccaat ggggaacgac
    15001 catctttggt attctctata aaatctttgc taaaaaggtc aatgagaaat ttagccttgc
    15061 tctttacctg attatgggct ggttggttct ggctatcatt cctgccatta tcagtcaaac
    15121 gacacccgtt ttctggagtc tcatggtaac tggcggactc tgttatacag ttggagctgg
    15181 attttatgcc aagaaaaaac cttatttcca catgatttgg catctcttta tcctagctgc
    15241 gtccgcactt caatacatcg ctattgttta ttacatgtaa aaaagttgag aaattcaatc
    15301 tcaacttttt tctttacaca tattgataaa gtactggtgc aagcgcacat catcagtcaa
    15361 ttctggatga aaagaactta ccaacatatt tttttcttgg gctgcaacaa tttgattgtt
    15421 cactgttgct aaaatttcta caccctcacc aacactactg ataatcggac cacggataaa
    15481 ggtcattgga atcttgccaa ctcccttaca ttctgcttcc gtgtagaaac ttcctaattg
    15541 gcgcccataa gcattacgct cgaccaccat atccatagtt cctagatgac tctctttctg
    15601 agaagtgatt tccttagcca gcaaaattaa gcccgcacag gtcccaaaca ctggtaagcc
    15661 agatagaatg gcttctcgga tgggaagtag catgttctgg tcacgtaaga gcttgcccat
    15721 ggttgtagac tcaccaccag gcaaaatcaa acccgacaag tcactctgat cttgctgaaa
    15781 atcatctaga tttctgagtt ctacactctc gacacctaat tgatctagca cttttgcatg
    15841 ttctgcaaag gccccttgca aggccaatat tccgattttc atctattttc ctcgttcagc
    15901 catgagaatt tggatttcat tttcattaat accaaccatg gcttctccta aatcttcaga
    15961 gatttgagct aggatttgag gattacggaa gttagtcaca gccttaacaa tggcactcgc
    16021 tcgtttaaca ggatctcctg acttgaaaat acctgaaccg acaaagaccc cctctgcccc
    16081 taattgcatc attaacgcag catctgctgg cgttgcaaca cctccagcag cgaaatttac
    16141 aactggcaat tttccatgtt catgaacata ttggaccaat tctacaggga cttgcaaatc
    16201 cttggcagca acataaagct cgtcctcacg taagttttga atgcggcgaa tttcctgatt
    16261 catcatacgc atatgacgaa cagcttggac gatatcccct gtccctggtt ctcctttggt
    16321 acgaatcatg gaagcacctt cagcgatacg acgcaaggct tcacccaaat ccttagcacc
    16381 acagacaaaa ggaacttgga attctttctt gtccacatgg aaacggtcat cagctggaga
    16441 tagaacttca ctctcgtcga tataatcaat ttcaatagcc tctaaaatct gagcttcaac
    16501 aaaatgcccg attctgacct tagccattac tggaatacta accgcttctt ggatttcctt
    16561 aatcatcttt gggtcgctca tgcgggaaac tcctccagct gcacgaatat cagccggaat
    16621 tcgttccaag gccatcacag ctgccgcacc agcagcttct gcgatacgag cctgttcagg
    16681 attctgcaca tccataataa caccaccctt gagcatctgt gccaagtttt tatttaattc
    16741 ataacgattt tcagtcattt gttttatcct catcatttgt attggtagct accttttcat
    16801 tttaagtcag aacagaatga agcacaagat aaaaaaacta taattgtatg gggacaaatt
    16861 agctaaaagc tatctgacct taacttaagg ccagatagct cattcatttt tatttttcag
    16921 ccgtaagggc agccattgtg atgtagttgt atggtttgtt gaagtgtggc aagaagaaga
    16981 ggtctgtcaa tgccaattta tcaattgtca catgctcttg gatagcaagt gagaacatgt
    17041 ggattcccat gctaattgca atatcatgtg aaaccatttg ggcaccaaga atttcacggc
    17101 tatctttgtc aaagacaatc ttaattgcta cttcatggtt gtcatgtttc atgaattctg
    17161 gtttttgaag atcgttaaag cctgtttcag ttgcgttgta accagcagct ttcgcttttt
    17221 caagagtcaa accagttgaa accatgtgaa gaccgtagat tgagatacca tttgaacctt
    17281 gaacaccgat tccttccaat tcatgtccac aagcattgta ggcaccaacg ataccagtgc
    17341 gaacagcatt tgaagcaaga gcgatatagc ttgtatcttt acgagcattg tcataaacag
    17401 tcgcacaatc accaacagcg taaacgcctg ggatagatgt ttcttgtttc ttgtctacaa
    17461 ggaaggcacc gttgcggaag agttcgatct taccatctgc aagggctgtg tttggacgga
    17521 aaccaactgc aaggataacc atatccacgt caaagctttc tttgtcagta atcaagcgtt
    17581 caactttacc gtcaccttcg attgctttaa cagtttgacc tagagccaag cggatgttgt
    17641 gatcttccaa gttcttcgcc atcatttgtg tgaagtcttt gtcatagtaa ccgttcaaga
    17701 cagtatcaac gatatcaaca aggacaactt cttttccaag acgttcaaag gcttcagcaa
    17761 gttcaacacc gatgtaacca ccaccaacaa cggcgatacg gtcgaggtgt tggctcttgt
    17821 cagaaagttt attgataact tcttcagcat tttggtacaa tttcacgaat tgtacgtttt
    17881 caagagttgc tttaaattcg cggtttcctt taacaatttc aacaccttcg attggtggca
    17941 agattggtgt agaacctgta gcgaaaatca atttttcgta tgattctttg tgctcttttc
    18001 cttcaacttc cgctgtaact actttgttat catagtcgat tgaaagaaca ggtgagttca
    18061 tgtaaacttt agcaccttta gcttccaatt tttctttatc agaatagaac aagccttcag
    18121 caccgtcaat ttgttcacca atccaaagag ccattccaca tcctaggaaa gagatgttag
    18181 agttttggtc aaatacaaca atttcgttct catttccaaa attatccaac atggtattga
    18241 tacatgctgt accagcgtgg ttagcaccga ctacaacgat tttactcata aaaatttcct
    18301 gccttcattc taaatttacc ttactagtat accatttacc gttagcgttt acaagggggg
    18361 tactattatt tcagcatttt ttatcaaaaa taattcccca tttatcctgt tttcaagcct
    18421 catgaatttt atgagcatat ttcttgactt tttccaaatt ttgtttgtga aaattctaac
    18481 tttatggtat tataataaca tgaaaataaa atataagggc ttcaatttag atgattattt
    18541 gatattacaa cttattttct ataagagaaa ggagttggta gcttgactct tcgttcacat
    18601 tctgaacggc taatggggac tactatcact atttcattag tggatgagca agccgatatc
    18661 tttctccaaa aatcctttga cttgctcaaa gagcttgaat accgcttcaa cgccaatagt
    18721 caagaatctg agttgatgga aatcaattat caagcaggaa tagcccccgt cacggttcat
    18781 ccagacctgt ttgagctgat ttcacttggg ttagagcata gcctagcgct ctctagccac
    18841 ctcaatatca gcattggtcc cttaattcaa acctggcgta tcggtttttc agatgccaag
    18901 gtcgcccagc ctcaagaaat tgaatcggtg ctgcctctaa tcaatcctca tggtatcgag
    18961 ttagattctt ctacttctac tgtgttttta aaacagaaag gaatgaagat cgatctaggt
    19021 tgtttagcca aaggatacag tgcggataag gttgcccaat ttcttaggaa agagggggtg
    19081 acttctgcct tgatcaatct gggagggaat atcctgacca ttggaaaaaa tcaggcaaga
    19141 ggggataacc catggcaaat cgggattcaa gacccagcca atcctagggg aaatcattta
    19201 atgaccatcc ctgttgtcaa taaatctgtc gtgacttcag gcatttatga acgtcacctg
    19261 accgtcgatg gacaagatta ccatcacatt tttgacagtc aaacaggata tcctgttgaa
    19321 acggaactag cgagtctaac aatcatctct gataaatcag tcgatggcga aatctggacg
    19381 actcgactct ttggagaaag acctgcttct atcctctggc aagtcgaaag tttggagggc
    19441 atcgaagtca tcctcatcga taaagaaggc cacctaagct gttcttcagg aattcctact
    19501 ctatagagac aactgtttca atggaatttt aaaattgtat tatgtaaaaa gagaaaggaa
    19561 atctcatgtt aaaacttatt gctattgttg gaacaaattc aaaacgttct acaaaccgtc
    19621 aattgcttca atacatgcaa aaacacttta ctgacaaagc tgaaattgaa cttgttgaaa
    19681 tcaaggccat tcctgtcttc aacaaaccag ctgacaagca agtacctgct gaaatattgg
    19741 aaattgctgc taaaatcgaa gaggcagatg gcgttattat cggtactcct gagtatgatc
    19801 actctattcc agctgttttg atgagcgctc ttgcttggtt gtcttatggt atttacccac
    19861 ttttgaacaa accaatcatg attacaggtg cttcttacgg tacgcttggt tcatctcgtg
    19921 cccaattgca acttcgtcaa atcttgaatg ctcctgaaat caaggcaaat gttcttccag
    19981 atgaattctt gctctcacac tctcttcaag catttaaccc aagtggcgac ttggttgacc
    20041 ttgatgttat caagaaattg gatgccatct ttgatgactt ccgtatcttt gtaaaaatca
    20101 cagaaaaatt acgtaatgca caagaattac ttcgcaaaga tgctgaagac tttgactggg
    20161 aaaatttgta agataggaga ccaaaaagaa tgaaatttgt tggacttgtt ggatcaaact
    20221 acgatcaatc atataaccgt aagctcttgg aatttatccg tcgctacttc aaactcaaat
    20281 ttgaattaga agttcttgaa attgacgaag ttccaatgtt taaccaagat gaaaaatggg
    20341 atgaaagttt ccaattacgt tatttatata acaaaattac tcgtgcagat ggtgttatca
    20401 ttgctactcc tgagcataac cacactatct cagcttcact caaatctgta cttgaatggc
    20461 tttcatacga agttcatcca tttgaaaaca agcctgttat gattgtgggt gcatcatact
    20521 atgaccaagg aacatcacgt gctcaagttc accttcgtaa aatccttgat gctccaggtg
    20581 ttaatgccta cacgcttcca ggtaacgaat tccttcttgg taaagctaag gaagcttttg
    20641 ataacaacgg aaatatcacc aacgaaggca ctgttaaatt ccttgaaact tgcttagata
    20701 actttgttaa atacgtagga gtcgtttcga aattgaaaaa accaaaacca atcgaatcag
    20761 aagacttgga ttgtggaaaa ccaatcgcta caaccatcac agaagttgac cctgacgatc
    20821 cagaatgggt agaaaaagtt gcagcaatca ctggtgctgt ttctggtgat acctatgtca
    20881 aattggacca cggtatccta acagttaacc aaattgatat gttcttgaaa gctatgccat
    20941 ttgaattgac atacgctgac gacaacaacc aattcctcta ctacaacaac gctcaccaag
    21001 atccagacac tatgttcgct aaacgtgtac cacctcaatc aggtagccgt atgtcgactg
    21061 ttcatggttc tcttccacca gcacgtatga agaacgtaga gtgggttatc ggaacacttc
    21121 gcaacggaaa ccaagaatac gtccgtacga tcgttccagg ttctcctgca ggtgttatca
    21181 acactcacaa ctaccaagca atgtactatc ctgatggatc atacgctggt atcaatgaaa
    21241 tcgtctttaa cttccaacca tggcttgact ggtacctaaa agagactggt caacgtttgg
    21301 tgggtggtag cggaccattt gctcctgctg ccggaagtca tggagacgcc gatgctactt
    21361 ctggcgcttc tgattcaggg gatgctggag gccacggtgg tgacgcagac gctacagccg
    21421 gtgcaagtta ctaatgatag tttttaggaa ccattatggt tcctttttta gcgaaaaaag
    21481 actagcaaac acaaactgct agtctctttg ttatcgtttc atctatcttt gttaaaagaa
    21541 agaatacata ttatgaattt ggatcatcaa gactacggcc atgtaatcct ttttcacgtt
    21601 gtacttggcg tagtttttct ggtgtaacat cgtttccttc ttcgtccaca attttgattc
    21661 cttcaatgtg gtgacgaaca gcgcggcgat aaccttcgat gtactcctca cgtagtttgg
    21721 cttgttccac tttttcttct ggtgttaagc cttctgtttt tttcttttta gcaagctcat
    21781 tgatacgagc aatttttttc ggatccattt cttctcctct gtaataagat aaagtccgtt
    21841 tatcaaattt tatttagtgt taatttggtt aaaacaagca aacttagctg ctttcttggt
    21901 tagttgtgac aagattgcca aacgattttg acggacagcc tgatcttcag ccattaccat
    21961 agtattttca aagaaagcat caatgactgg gctaagcgca aagagttgtt tcaattgctg
    22021 acttgcaggt cctgataaaa cgagtgtttc tactgcttct gccaaagctt tttcttggtc
    22081 attctcaaat agtgctgaat caaccgtagc aaccccttct gccttctcag ccaggttaaa
    22141 ggcacgagaa agtgattcaa cagatggttt aaaatcttct tccttgctta cttctacgag
    22201 agcacttgct gcttccaaca tatctgccac aacaaagttt gaacctgcaa gaactgcttc
    22261 tttgatatct tttggagtag agcccatcat cttatcaaca cgagccttga taaagtccat
    22321 aacctctgct ttattttcat aagtcaaact gtcaaatttc aatgcataaa ggctatcaat
    22381 cagctcatcc atagcaatgt gccaaccaaa ggcatccaag atacgaacca caccttgagt
    22441 tgcacgacga agggcataag ggtcattaga acctgatgga atcaatccta ctgagaagaa
    22501 actcaaaatc gtatccaatt tgtctgcaat ggctagaact gcgccgacct tgctctctgg
    22561 aagttctcct tcagctgatg taggcatgta gtgttcacga atagcagctg ccaccgctgg
    22621 agtttcacca gcaagaaggg tgtatttttc acccataatt ccttggagtt cgtcaaattc
    22681 accaaccata cctgtcaaca agtcaaactt gtaaatggct gctgcacgag caaggtcaac
    22741 tgtttcatcc actgacaaac cagctttttc tgccaaaagt acagtgattt gacccgtacg
    22801 aatcatgtgt tcacgaaggg aaccaatctt ctcatggaag gtgacattgt ttaatttttc
    22861 aacaagatct gaaatcacca atttttggtc ttcacgccag aagaattctc cgtcttccaa
    22921 gcgggctacc aagacttttt catttccttt gatgacattt ttcaaacgct ctgcgtttcc
    22981 gttacgaaca gaaatgaagt ttggcaagag ttttccatct tgatcacgaa caacaaagta
    23041 acgctggtgt tccttcatag aagtcaccaa gacttcttct ggaacttcaa ggtatttagc
    23101 atcaaaactt cccatgaagg cagttgggta ttcaaccaaa ttcaagactt cattcagcaa
    23161 atccgcatca atttcgatat gtacaccatg ttttgcctca atttccttga tttggtcaac
    23221 aatcatttgc tcacgttcac atggatctgc gattacaaac tgcttacgaa ggtcttcttc
    23281 atagctcaat gctgactgaa ttttggtttc tttccccaag aaacgatggc cacggctcac
    23341 acgacttccc ttgatatcaa ggaaatccaa gtcaaactct tgctcatcca agagaacagt
    23401 taaagtgtga acagggcgga tgtattcaaa gctatttcca gcccagtgca tgctgacagg
    23461 gaaagtcaat gacttcaaga catctacaac accaggaaca atggcttcaa ctgattggcc
    23521 aacttcttcc ttagtgacat agacatattc ttcaccctta atttcacgga attcgatatc
    23581 ttcaacagtc aaacctttcc cacggacaaa tccttgagct gctttggtga agtttccatc
    23641 actatccaag gcaattttct ttgctggtcc cttgaaatct tctgtcaaat cagactgttt
    23701 gtctgataaa ccagtcacac gaacagccaa acgacgtggt gttgagaagg tttgaatggc
    23761 ttcaaaagac aggcggtttt ccttgaggaa ggctgccatt ttttcgccta gttgtttttc
    23821 acttggtgtg acaacatagg ctggtaattc ttcaagaccg agttctacta ataagttttt
    23881 tgtcatgttt tttcctttac attttcttca atcttttttg atatgcacct taggtactgg
    23941 ggacgtcgct aactaactct gtgagtctgt atggaaatcc aatgctaaat ttatcaagat
    24001 ttccaacaat ctcatcaaag tggatttagc tgactgattt ttgaacctaa ggtttcaaaa
    24061 atccccacat aacagtacgg cggtgcatat ccctctagca agtcttcgac ttgcctctaa
    24121 cgattttaga gtacagtatt actcactatt ctgcgtcttc tgctaaaagt ttaactcgtg
    24181 tttcttcatc caaaagtggg taacctaggc gtttgcgttc tgcaacaaag gttttggcta
    24241 caacacgggc caagttacgg atacgagcga tatagcctgc acgctctgtt acagatacgg
    24301 caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt gagaacatag tcataggcag
    24361 ggtgtaccaa gccttcttct aatgcacgac cagcttcttt ttcaaactta tcaaagtttt
    24421 caagcaacat ttcttggtcc gaaatttcaa atgaatattt tgagtgctca tactcaggct
    24481 ggataaagat ttctccgtat tttacaccat cagcccactc gatatcatag acagagtcta
    24541 cttcttgaat gtaagaagcc aagcgctcca aaccataggt aacttccgca gtcacagggc
    24601 cagttgccaa tccaccgact tgttggaaat aagtgaactg agtgatttcc attccgtcaa
    24661 gccaaacttc ccaaccaaga ccagctgaac cagttgatgg gttttcccag ttgtcctcaa
    24721 caaaacgaat atcgtgctcc aaaggattga ttcccaattt ttccaaagat tcaaggtaaa
    24781 gttcttggat atttgatgga gaaggcttca tgaccacctg gaattggtgg tgttggtaga
    24841 gacggttagg gttttcccca taacgaccgt cagcaggacg acgtgatggc tctacataag
    24901 ctgcattcca tggctcaggt ccgatagcac gaaggaaagt gtaaggactc attgttcccg
    24961 cacctttttc attatcataa gcctgcataa gcatacaacc ttggtcattc caaaattgtt
    25021 gcaaagtaag gatgatttcc tggaaagtca atttcttaga cattgtttac tcctttttat
    25081 ataaattact tgcgacatga gtctgcctcg tcgatcatac gaggcaagac gagttagtac
    25141 tgcgtctagc tcaggcactc tagtgctgag cgtggggcag tccgactgta aggaggtttc
    25201 tgccactgac gcagtgaaat gtcaatttct tagacattgt ttactccttt agtttttata
    25261 tttctttttc aattaatttc tgctgaacaa accaatctaa caagggaaaa gttttttctg
    25321 tatttgccca taaatgataa gaatctaaga ccgcttgtac actacctaaa ttttctacca
    25381 acttatctac tgtcaaaaag ctgcgccagt attcataaaa aatacttgca tagttcttat
    25441 cataagtcga agaaccaaaa tcattcaatg aatgccaacc atacttcttc tgaaaaagtt
    25501 ctacgagaga ttgattacaa attttttccg cttgaaattc ctcttctgtc aagaaatact
    25561 tgcgactaat atattcaacc atcccctctt cgaaccagat ataagagtca taaccatcaa
    25621 aatcatctaa aaaatgctcc gaccaatgag ctaactcatg tcctacaatc tgtaagagga
    25681 aattttcaga taaagaatga tagtgacttt ctattgcttg agtttgctga gaacactcgt
    25741 agtccatcaa ttgatacaaa tacaactctt tccaaacagc taaatcaggc gccataacca
    25801 ttcgtctatt atttgtatag gctggaacag cgctttccct aataatctgt gtagcagcat
    25861 taaaatctga ccaaataacg gcttgcggta aatcacagac tgcaagttca ttctttaaaa
    25921 aagattgata atctttcaac ttttcagtat ttcttactac aaaatcacga aatgtagcta
    25981 gttgactatc gtcttttaca agataaaggt tgtccacggc tctcctttct ttcacacaat
    26041 agactgaata agcaaaagaa ccgcgtccga caatcctagg ttgatttaac ctaggggcgt
    26101 cagaaacgcg gttccaccct aatttattac ttcattgttt ttgaaaatac tacagaaagc
    26161 gccagctcat tattttgccc tacttggctc ccactatccc aagctcgctt gaagaacgcc
    26221 ataagacttt ctttcctgct gactattata tcaaaaatta gaataatgta caattctttt
    26281 tatgattttc tagaaatcca catcatcaac tcgtggagca cccgattgaa cagcaatcgt
    26341 ttttaagttt tctctctcct catgactcag ttcaattcca aagcaatcaa gattagcttg
    26401 aatacgagag gttgtgacag attttggaag tggtaaaaat ccttctgcca agctccaggc
    26461 caaggctatc tgagcaatcg attttccgtg atttgctgcg atttcttgca cttgcttgct
    26521 atcaaatagt tctccctgac caaaaggccc ccaagcttcc aataaaattc ccttttcacg
    26581 acagtaagct acgacttgat cttgatacac acctggcgcc aagcgaactt gattgaccgc
    26641 aggaacgata gttgcagttt caagcaaggc atccaaatga tggggaagaa aattgctaac
    26701 gccgatagca cggattttcc cttcttgata gaggtcttcc atcgctctcc aaacttccgc
    26761 attgcgagtt ttccatgcgt cattttctct gagcggtttt gggttcggcc aatgaatcaa
    26821 atacaaatcc aaataatcca agcccagttt ttctatagat ttttccaaag cttgacgagt
    26881 ttgctcatag gtttgctgac tattccaaag cttggtagtt acgaacattt cttcacgtgg
    26941 aactccgcta tctttgattg cttgaccaac acttttttca ttctgataaa tcgccgccgt
    27001 atcaatatga cgataaccag ccttcaaggc ttctaacact gcacgatagg cttcttctcc
    27061 atccttagcc ttaaaagttc caaatcccaa tactggaatt tctactccat tatttaattg
    27121 ataggtattc atatgcttct ccttctattt tattcatcaa ttatagcata aaacattttt
    27181 taagtagtac tatcttctgt aaaaaataag tctgtcaaat atctaacaga cctatttttt
    27241 cttgcttatt catcacgact atagtacagc tcatgagctt ttaggcgagt attgagcatc
    27301 tctggtatgg tcacaaaggt ataaccttga tttttcaaat actcaatgac ccttggcaag
    27361 gcattgactg tcggactgtg aatatcatgc atcaaaacga tagagccgtt agctacttga
    27421 tgctgaattt ctgtcaaaat agatgcttca tttttactct tccagtccag actatccaca
    27481 tcccacatga taaagctcaa atccaagcta ttgcgaatat catctgtaat agcaccataa
    27541 ggtggacgca tgagtttaga actagaaccc agcactttag ttagcacatc ctcagtatca
    27601 gtaatctgct ttttagcttc atcaagagag agttgcgaga gaatcggatg gctccaacta
    27661 tggtttccaa caacatgacc ttcagatttt atcctcttca ccaagtcctc attcccagaa
    27721 acatttttcc caagcacaaa gaaagtcgct ttaatatcat atttagctag ggtctctaat
    27781 acctgcgggg tcgttgctgg atttggacca tcatcaaagg ttagagcgac aactttttga
    27841 tatttcttat caaagtaaga ttggtacaag gccgcatctt tttcgagtaa gtacgaagat
    27901 tggataacat caaagaaagc agatactggc aaggctatct cttctaaatt ttcaaccaca
    27961 ggacttggat aaaggataat ctgactatcc ttgtaatcaa aattccatgc agacaagtct
    28021 tggtcagaga agttttttac aatctgctca ctctggtctt gctctatttt tttatcctct
    28081 atgaaggagg tcaactcttt tatcagctgt tccttagcct tactagcatc tgaaaacagt
    28141 tggtcaagtg taaaaggttg gccatcttct gttaagtgaa cctttcctag actggtcttt
    28201 tcagtctctt caaccttaaa agcaactaaa tcatagattt gtttggtcac attacgatta
    28261 acgactccct ttaaacctga ctcttcttgc tctgtatagt agaaaacaag attgtcctta
    28321 ctttctagct tgtccttaac atcttgattt atcaactccc taacagagga aatcactttc
    28381 tccccttgga gaggataata ggcaatcact tcggcttgcc cctgacgaaa atgctccttc
    28441 tgatttccct cactcaattg atcatctttc tcttttttga gcgattcaat cttttgctca
    28501 aaactttttt gctggtagat cttaaaagca ataaatccgc ctaataggca aatagaaatt
    28561 gctaaaatac caatcaaagc caataataca tgtctcgttt tcccgtgtct gccacgtcct
    28621 agtctacttt tattcatatt tatatcataa caaatttgag caataatttc aatgatacaa
    28681 tttggcaatc cacaattcct ctagcaagcc aaagcatctt acatcaactc caacatccgt
    28741 atctgtagag attatgtagt tttctatcta tggaatttct agtagattga agctagaata
    28801 gtagacctat acttctaaaa cattgttaga aatcgatttg attgtcctga tcgatttgtc
    28861 ctgttctcat ttcattttac tataaaacta tgaaaagcta ggtcatctga gaagtaatta
    28921 ctatcgacaa ttgcgatagc gtatactggt gcgatttgtt tgtagctctg gtgggtatca
    28981 ccttcacgtt ggcgaatttt ttctaggttt tgattaacct gactgcacag ataagcccat
    29041 aggcgattga tgaaaaaatt ctgatgatga acctgaattt cgatgataac ttggattcca
    29101 ttatctaact ccgccaagac gtctatacta gtatagaaat cttgcgctga gtagggcatg
    29161 gaaggcaaga cgtgaatgtt acttccctcc aaaatcgtca cattttttgc tggcaagtcc
    29221 agcatatcgc ggataaattg acaaatcatt agttgggctg atacctggat gtctgagaat
    29281 cctccttttc ctccttctat tatagcacat ttttaaatct aggtttgcta gattctatgc
    29341 ttctatctat ttattcggaa aagaaagcta atagcttatt tgaaattttc tcctctctgt
    29401 tggatagtcc cgtctatcta tgttataatg aaagaaggat ttaaaatcgg aaaaggagta
    29461 tttatgctaa aattaggtgt catcggaact ggcgctatca gccatcattt catagaagca
    29521 gcccatacca gtggagaata ccaactggtc gcaatctatt ctagaaaact agaaactgca
    29581 gcaacctttg cttctcgcta tcagaatatc caactattcg atcaattaga agtcttcttc
    29641 aaatcttctt ttgatttagt ctatatcgcc agtccaaact ccttacattt tgctcaggca
    29701 aaagctgcct tgtctgctgg taaacatgtt attctcgaaa agccagctgt cactcaacca
    29761 caagaatggt ttgatttgat tcaaacagct gaaaaaaata actgttttat ctttgaagca
    29821 gctcgtaatt accacgagaa agcctttact actatcaaaa actttttagc agataagcaa
    29881 gtgttaggag cagatttcaa ctatgccaaa tactcttcca agatgcctga cttgttggct
    29941 ggacaaacac caaatgtctt ttcagaccgt tttgctggtg gagccctcat ggatttgggg
    30001 atttatcccc tctatgctgc tgttcgtctt tttgaaaaag ctaatgacgc gacttaccat
    30061 gctcaacagc ttgacaatag cattgaccta aatggagatg gtatcctctt ctacccagac
    30121 tatcaagttc acatcaaggc tggaaaaaac atcacttcca atcttccttg cgagatttat
    30181 acaacagatg gaaccttgac tctcaacacg attgagcata ttcgctcagc tatttttacc
    30241 gaccaccaag gaaatcaagt ccagcttcct atccaacagg ctcctcatac gatgactgag
    30301 gaagtcgctg catttgcaca catgatccag caaccagatc tgaatctcta ccaaacttgg
    30361 ctgtatgatg caggctctgt tcatgagcta ctatatacca tgcgccagac tgctggcatt
    30421 agatttgagg cagaaaaatg aaaaccaaac taccgactga atggcaagaa ctgagtgacc
    30481 aactcggttt ccaagaattc acccccattc aaactcaact atttgagccc cttcttgctg
    30541 gagaaaacct cctaggagtg agccaaaccg gaactggtaa gaccctagct tatctcctac
    30601 caagtcttct cagactacaa aagaaaaaag cccaacaact cttgattcta gcaccaaata
    30661 cggaacttgc tggacagatt tttgatgtat gtaaaacgtg ggcagaagct atcggattga
    30721 ctgctcaact cttcctttca ggttcaagtc agaaacgaca aattgaacgt cttaaaaagg
    30781 ggccagaaat tctgattgga actcctggcc gtatctttga actcattaaa cttaaaaaaa
    30841 tcaagatgat gaatgtggaa accatcatcc tggatgaatt tgaccaatta ctagatgatt
    30901 ctcagattca ctttgtagaa aaaatcactc actacgcacc tcgtgaccac caactggtct
    30961 acatgagtgc cacgaccaag tttgatcaag aaaagattgt accgaataca cgcactattg
    31021 atctctctga tcaaaaattg gacaatatcc agcatttcta catgcaggta gaccaacgtc
    31081 atcgagtgga tatactacga aaactggctc atgtagagga tttccgtggt ctagtcttct
    31141 tcaatagcct gtcagacctt gggaatgccg aggaaaaact acagtatcgt gatatattgg
    31201 ctgtttccct cgctagtgat gttaatgtta aatttagaaa agtcatctta gaaaagttta
    31261 aagataacca actaaccctg cttcttgcaa ctgacctttt ggctcgtgga attgatatcg
    31321 atagcctaga atgtgtcgta aactttgata ttcctagaga tagcgaaacc tacacacacc
    31381 gtgctggccg tactggccgt atgggcaagg aaggatatgt tattactctc gtcactcata
    31441 cagaagaaat caaaaaactt aaaaagtttg caagtatccg agaaattgtc ctaaaaaacc
    31501 aagaactcta tatcaaataa agccccattg ctctatacac atccagtgta tggagctttt
    31561 ttacaagcaa ttcaatttta tactcatctg ttttcaaaaa gcattctaat ccatctccga
    31621 ttaacgatgg actttatcac ctccttctcc agttcttgta taatatcttg aagttgattc
    31681 atgacatctt ccaaagttcg aaaggcttta ttcttaaatc cacgtttacg aatctctttc
    31741 cacacttgtt caacggggtt tatctctggt gtgtatggag gaataaatgc aaagccaata
    31801 ttagtcggaa tttttaaggt acttgattta tgccatatag cattgtccat aacgagtaaa
    31861 agataatcat ctggataagc ttgtgaaagc tcttctaaaa aggcgttcat ccactcagta
    31921 ttacatccac cagct
//
LOCUS       DAVHIT010000018        30654 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVHIT010000018 DAVHIT010000000
VERSION     DAVHIT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.018"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            110..763
                     /locus_tag="VVN16_000677"
     CDS             110..763
                     /locus_tag="VVN16_000677"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5572118.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            773..1237
                     /locus_tag="VVN16_000678"
     CDS             773..1237
                     /locus_tag="VVN16_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU5572119.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTADLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1250..2518
                     /locus_tag="VVN16_000679"
     CDS             1250..2518
                     /locus_tag="VVN16_000679"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5572120.1"
                     /translation="MLLIKNGHVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2532..2792
                     /locus_tag="VVN16_000680"
     CDS             2532..2792
                     /locus_tag="VVN16_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572121.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2803..4164
                     /gene="pdrM"
                     /locus_tag="VVN16_000681"
     CDS             2803..4164
                     /gene="pdrM"
                     /locus_tag="VVN16_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU5572122.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4318..5286
                     /locus_tag="VVN16_000682"
     CDS             4318..5286
                     /locus_tag="VVN16_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5572123.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5302..6294
                     /locus_tag="VVN16_000683"
     CDS             5302..6294
                     /locus_tag="VVN16_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5572124.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6512..7555
                     /locus_tag="VVN16_000684"
     CDS             6512..7555
                     /locus_tag="VVN16_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU5572125.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYKVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7619..9304
                     /gene="lpdA"
                     /locus_tag="VVN16_000685"
     CDS             7619..9304
                     /gene="lpdA"
                     /locus_tag="VVN16_000685"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5572126.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9368..10357
                     /locus_tag="VVN16_000686"
     CDS             9368..10357
                     /locus_tag="VVN16_000686"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU5572127.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAKINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="VVN16_000687"
     CDS             complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="VVN16_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU5572128.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(11994..13544)
                     /locus_tag="VVN16_000688"
     CDS             complement(11994..13544)
                     /locus_tag="VVN16_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5572129.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGAICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(13560..14339)
                     /locus_tag="VVN16_000689"
     CDS             complement(13560..14339)
                     /locus_tag="VVN16_000689"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5572130.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(14326..15177)
                     /gene="ylqF"
                     /locus_tag="VVN16_000690"
     CDS             complement(14326..15177)
                     /gene="ylqF"
                     /locus_tag="VVN16_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU5572131.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LIKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(15397..15573)
                     /locus_tag="VVN16_000691"
     CDS             complement(15397..15573)
                     /locus_tag="VVN16_000691"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5572132.1"
                     /translation="MQAGIPPDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(15625..21639)
                     /locus_tag="VVN16_000692"
     CDS             complement(15625..21639)
                     /locus_tag="VVN16_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5572133.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLVELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTKLINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKKLELRNVSDIELYSQTNGTYR
                     QHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNGQSVYKITAKAEKLQQEL
                     ENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRYAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPFILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIAEVKAKQKQAGTKYSIGVYDRITSNTWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEAKFDSSNPAMKNFFGPVG
                     NKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVLDPTERFQNAADLQNYVH
                     NMFDLIYMMEYLEGQSIVNKLSVYQKIEALRKIENQYLTDPADGNDVYATNVVKNLTE
                     DEAKKLTSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAPIFSALSGEKGTPGDLMG
                     RRIAFELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGKERGLVTDELVLKKVFDG
                     KYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPRYGTKTINNVDELQKLMDEAVL
                     QDATGTRWSNYNPETDSAVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            complement(21848..23026)
                     /locus_tag="VVN16_000693"
     CDS             complement(21848..23026)
                     /locus_tag="VVN16_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572134.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(23241..26891)
                     /gene="addA"
                     /locus_tag="VVN16_000694"
     CDS             complement(23241..26891)
                     /gene="addA"
                     /locus_tag="VVN16_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU5572135.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGNLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVTFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(26888..30163)
                     /gene="rexB"
                     /locus_tag="VVN16_000695"
     CDS             complement(26888..30163)
                     /gene="rexB"
                     /locus_tag="VVN16_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU5572136.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDIDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDSFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDIERDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            complement(30363..30518)
                     /locus_tag="VVN16_000696"
                     /pseudo
     CDS             complement(30363..30518)
                     /locus_tag="VVN16_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         8560 a         6135 c         6355 g         9604 t
ORIGIN      
        1 attcaaatcg tatatagtcg atttattaaa tctttagata aattgtaaaa agtggtttcc
       61 atagccactt tttgctataa tagaggcagt aaaaatgaag gagtcagcta tggaacactc
      121 gtcttggcat gctttgatta aggcgcaatt acctgagggt tatttcggga aaatcaatca
      181 gtttatggag caggtctatt ctcaggggat tatttatcca cccaaggaaa aggtttttca
      241 ggctctcttg acaacactgc ttgaagaagt taaggtggta attctagggc aagaccccta
      301 tcacggacca ggtcaagcgc agggcttgag tttttctgta cctgactcta tcccagctcc
      361 accatccttg caaaatatct tgaaagaatt gtcagatgat atcggagtta agaaatctca
      421 tgatttgaca gcttgggctg agcaaggagt cttgcttctt aatgcttgtt tgactgttcc
      481 tgctggacag gccaatggtc atgctggtca aatctgggag ccttttactg atgctgtgat
      541 tcaggtggtc aatcatctag atagaccagt cgtttttgta ctctggggag cttatgcacg
      601 taagaagaag gccttagtta ccaatcctca tcacttgatt atcgaatcag cccatccaag
      661 tcctttgtcg gtttatagag gattttgggg ttccaagccc ttttccaagg ccaatgcatt
      721 cttaaaagag acaggacaag agccaatcga ttggcttaga taaggagaaa atatgcctca
      781 gttagcgacg atttgctaca ttgataatgg gaaagaactg ctcatgctcc atcgtaataa
      841 gaaacccaat gatgtccatg aagggaaatg gattggtgtg ggtggtaagc tagagagagg
      901 agagacgccc caggaatgcg cggcgcgtga aatccttgaa gaaacagggc tcaaagccaa
      961 gccagttcta aaaggtgtca tcacttttcc tgaatttaca gcagatttag actggtacac
     1021 ctatgttttt aaggtgacgg agtttgaggg cgacttgatt gactgcaatg aggggacgct
     1081 agaatgggtt ccctatgacg aggttttgag caagccgact tgggaaggtg accacacctt
     1141 tgttgagtgg cttttagagg ataaaccctt cttttcagcc aagtttgttt atgatgggga
     1201 taaattgttg gatacccaag ttgatttcta tgaataaagg agaaagcaga tgctactaat
     1261 caaaaatggt catgtaatgg atcccaagtc tggtttggat caagtttgtg atgtcttagt
     1321 tcaagatggg aaaattatca aaattgcgcc tgagatcacg gaagaaggag cagaaacgat
     1381 tgatgctact ggtcttgtgg ttgctcctgg tttggtcgat attcatgtgc atttccgtga
     1441 acctggtcaa acacataaag aagacattca tactggtgcc ctagcagccg ctgcaggtgg
     1501 ttttactact gtcgtcatga tggctaatac cagtccaacc atttcagacg tggagacttt
     1561 gcaagcagtt ctccagtcag ctgccaaaga gaagattaat gtcaagacag ttgcgaccat
     1621 tactaaaaac tttaatggta aaaacttgac tgactttaag gcgctcttag aagctggtgc
     1681 ggttggtttc tctgatgacg gtattccgct tgagagcagt aagattgtca aggaagccat
     1741 ggaggaagcc aaaaaactca atacctttat cagccttcat gaggaagatc caggtttgaa
     1801 cggtgttctt ggctttaatg aaaatattgc tagagaacat ttccatatct gcggtgctac
     1861 tggggtggct gagtacgcta tgatggcgcg tgatgtcatg attgcctatg caactaaagc
     1921 ccatgttcac atccagcatt tgtctaagga agaaagtgtt aaagtagtgg agtttgctca
     1981 ggggttaggt gcagaagtca cagcagaagt agcgccacag catttctcta agacagaagc
     2041 acttctttta acacaaggta gcaatgctaa gatgaatcca ccgcttcgtt tggaatcaga
     2101 ccgtcgtgcc gttatcgaag gtctcaaatc aggtgtcatc acagttattg cgactgacca
     2161 cgcgcctcat catgtagatg aaaaaaatgt tgaggatatt accaaagcgc catctggtat
     2221 gactggctta gaaacatctc tgtctctcgg cttgacctat ttagtagaag ctggtgagtt
     2281 gagcttgatg gaattacttg aaaaaatgac atacaaccca gccaagcttt acaactttga
     2341 agcaggttac ttggctgaga atggtccagc agatatcact atttttgatg ccaaggctga
     2401 ccgccttgtg gactcccatt ttgcttccaa agcagctaat tcaccattca tcggtgaaac
     2461 cttaaaaggg caggttaaat ataccatctg taagggacaa atcgtctatc aagcttgata
     2521 ggagttagaa tatgaattat tttcgaaaac gtagggagag acaagcgaaa tctaatagtg
     2581 gaatttactg tcccgcagct aatcgttcca aaattcaata tgaaactaaa gaaaaagctg
     2641 accatgcggt tcaatatgat aacggaggtt tagttagaag ctattattgt cgtacatgtg
     2701 cgtgttggca tactacttca aaatctaata aacctccgct aagtttgaaa aactatggtc
     2761 tgaaactctt tggtttagat aaatcagaat agaaagagaa gtatgtataa gacaaagtgt
     2821 ttacgagaga agttagtatt atttttaaaa attttcttcc caatcctgat ttaccaattt
     2881 gccaattatt ctgcctcttt tgttgatact gcaatgacag gtcaatacaa cactatggac
     2941 ttggctggtg tatctatggc aaccagtatc tggaatcctt tctttacatt tctaacaggg
     3001 attgtgtcag ccttggtgcc tatcattggt caccatcttg gtcgaggcaa aaaggaagaa
     3061 gttgcgtctg atttttacca atttatttat ttggccttgg gcctatctgt ggtcttgctg
     3121 gggatggtac ttttcttggc accaacaatc ttgaatcata ttgggttaga agcagcagta
     3181 gcggcagtag cggttcgcta tctttggttt ttatctatcg ggattatccc cttgttgctc
     3241 tttagcgtca ttcgttcctt gctggattcg ctgggcttga ccaaactgtc catgtacctc
     3301 atgcttttgt tactccctct caatagcgga tttaactatc tcttgattta cggtgccttt
     3361 ggtgttccag aactgggagg ggctggtgct ggtttaggaa catccttggc ctactgggtc
     3421 ttgcttggga tttctgttct ggttttattt aaacaggaga agctcaaagc cttacacctt
     3481 gagaaacgaa ttccacttaa tatggataac attaaggaag gagttcgttt aggtctgcct
     3541 attgggggaa ctgtcttcgc ggaagtggct atcttttcag tggttggctt gattatggct
     3601 aagttttcgt ccttgattat agctagtcac cagtcagcta tgaacttttc aagtcttatg
     3661 tacgcctttc ctatgagtat ctcatcggct atggctattg tcgtttccta tgaagtggga
     3721 gccaagcgat ttgatgatgc gaaaacctat attggtctag gaagatggac tgctctcatt
     3781 tttgcggcct tcaccctaac cttcctttac atttttaggg gaaatgtggc cagtctttat
     3841 ggtaacgacc caaaatttat cgatttgaca gcgcgttttt taacttatag tcttttcttc
     3901 cagttagcag atacctttgc ggcaccgctt cagggaattt tgcgggggta taaggataca
     3961 gttattcctt tttaccttgg tttgcttggt tattggggcg tagcaatccc tgtggctacg
     4021 ctatttgatt ccctaacaga ttttggagct tattcttact ggatcggctt gattattagt
     4081 ttgattgtga gtggggcgct ctaccgttgg cgtttaactg tgattatgaa gagatttgaa
     4141 tctttagcaa aatccaaacg ctaaaaagtt tgtgaaaaaa tattcttgac aagaaaatcc
     4201 cttgttttca cggggttttc ttttttattt tgtgaaattt cttttagaaa aactttgaca
     4261 gtatttgcca aaagcggtaa aatagtaagg taagaagaaa gttagaaagg caagaagatg
     4321 tcaactttag ataaaaatct tttgctagaa atgttccgta agatggaaga aatccgtcgc
     4381 atggacttaa aaattgcaca attagtaaag aaagggaaag tgccaggaat gacgcacttt
     4441 tctgttggtg aagaggcagc taacgtgggg gctatgttag ctctcaatcc agatgatctg
     4501 attacctcaa accaccgtgg acacgggcaa gctattgcta aagggattga cctcaacgga
     4561 atgatggctg aaatccttgg taaatacact ggaacctgta aagggaaagg tggatctatg
     4621 catatcgctg accttgatgc tgggaacctt ggtgccaatg gtatcgtagg tggtggtatg
     4681 gggatcgctg tcggtgcagc cctcagtcag caaatgcaaa ataccggtaa aatcgttgtc
     4741 tgcttctttg gagatggtgc gaccaatgaa ggtgttttcc acgaagcagt gaacatggct
     4801 tctatctgga acctgccagt cattttctat tgcattaaca acggttacgg tatctctgcg
     4861 gatatcaaga aaatgaccaa tatagaacat atccatcaac gtagcgccgc ttatggaatt
     4921 cctggaatgt tcatcgaaga cggtaacaat gtcatcgatg tctatgaagg atttcagaaa
     4981 gctgtagacc atgttcgcag tggcaatggt ccagtcttga ttgaaagtgt aacttatcgc
     5041 tggctcggtc actcatcatc tgaccctggt aaatatcgta cgcgtgaaga agtggaattg
     5101 tggaaacaaa aagatccaat cgaaaacctc cgcaattacc ttattgaaaa taacattgca
     5161 agtgccgaag aattggaaga aatccaagcg caagtaaagg aagcagtaga agcttctgtt
     5221 aaatttgcag aggaaagccc attcccaccg cttgaatcag cctttgaaga tatttacgca
     5281 gactaaggag aaagagataa aatggaaaca aaaacaatgt ccttccgtga caccattatc
     5341 cttgctatgt ctgaggaaat gcgtcgcgat gaaaatgtgt tcttgatggg agaagacgtc
     5401 ggtgtcttcg gaggagactt cggtacttct gttggaatgc ttgaagaatt tggtccagaa
     5461 cgtgtccgtg actgtccgat ttctgaagct gccatctctg gagcagcagc aggagcagcc
     5521 atgacaggac ttcgtccaat cgtcgatatg accttcatgg acttctctgt tattgccatg
     5581 gacaatatcg tcaaccaagc tgctaaaaca cgttacatgt ttggtggtaa aggtcaggtt
     5641 ccaatgactg ttcgttgtgc agctggtaac ggagttggct ctgccgccca gcactcacaa
     5701 tctctagagt cttggtttac tcacattcca ggacttaagg ttgtggcacc tggaacacct
     5761 gcggacatga aaggcctgct taagtcttct atccgtgata acaacccagt tattatactt
     5821 gagtacaagt cagaatttaa ccaaaaaggg gaagtgccag ttgatccaga ctacacaatt
     5881 ccacttgggg ttggtgaaat caaacgccaa ggaacggatg taacagttgt tacttatgga
     5941 aaaatgcttc gccgtgtggt tcaagctgct gaagaattag cagaagaagg aatttcagtt
     6001 gaaattgttg acccacgtac ccttgttccg cttgataagg atatcatcat aaactcagtg
     6061 aagaagactg gtaaggttgt tctggtcaac gatgcccaca aaacaagtgg ctatatcggt
     6121 gaaatttcag ctattatttc agaatcagaa gcatttgact atctagatgc accaattcgc
     6181 cgttgtgcag gagaagatgt gccgatgcct tatgcacaaa acctagaaaa tgcaatgatt
     6241 ccaacagttg aaagtatcaa agatgcaatc cgaaaaactt ataacaaaga atagagttac
     6301 ataagataag ttatgtaaat aaaataaagc tgacgagaaa ctttgtatct tttaggtgca
     6361 gagctcttcg tcattaatat cttagattag agcacgggct aaaagctcgg aaaaaagata
     6421 aatctccttg gcttcatcga agccatcgtc gatttcctat ttttcagtcg cttttgacgc
     6481 cctttgtatc ataaatagaa ttggagaggt catggctgat gacaagctaa gagcgactcc
     6541 tgcagctaga aagttagcgg atgatttagg gataaacctc tacgacgttt ctggctcagg
     6601 tgcaaacggt cgtgtccaca aagaagacgt ggaaacttat aaagacacaa acgtggttcg
     6661 catttcgcca cttgcaaaac gaattgccct cgaacataac attgcttggc aggaaatcca
     6721 aggaaccggt catcgtggta aaatcatgaa gaaggatgtt ttggccctgc ttcctgaaaa
     6781 tatcgaaaac gatagcatca aatctcctgc tcagattgaa aaagtggaag aagtccctga
     6841 taacgtaaca ccatatggtg aaatcgagcg tattccaatg acaccaatgc gtaaggttat
     6901 tgcccaacgc atggttgaat cttacttgac tgcgccaacc ttcaccctca actataaagt
     6961 tgatatgact gaaatgttgg ctcttcgtaa gaaggttctt gagccaatca tggaagcaac
     7021 tgggaagaag actactgtaa cagaccttct ttcgcttgca gttgttaaga ctcttatgaa
     7081 acacccatac atcaacgctt cattgacaga agatggcaag actattatca ctcacaacta
     7141 tgtcaatctt gccatggcag ttgggatgga taatggattg atgacacctg tcgtttacaa
     7201 tgctgagaaa atgagtcttt cagaactggt tgtagccttt aaggatgtta ttggccgtac
     7261 cttggatggt aaattggctc caagtgagtt acaaaattca acattcacaa tcagtaattt
     7321 gggaatgttt ggtgttcagt cctttggtcc gattattaac caacccaact cagctatcct
     7381 tggtgtcagt tcgacaatcg agaagccagt tgtcgtcaat ggtgaaattg tgattcgccc
     7441 aatcatgagt ttaggattaa ccattgacca ccgtgtcgta gatggtatgg ctggtgctaa
     7501 gtttatgaag gacttgaaag agttgattga aacgccaatc tcaatgttaa tttaagagca
     7561 agagtattca tttttaagat atagataaag gaaggaagac atggccttag aagtaattat
     7621 gccaaaagcc ggcgtggata tgacagaagg acaaatcgtc caatggaata aaaaagtcgg
     7681 agaatttgta aaagaaggag aaatcctttt ggaaatcatg actgataaag tcagcatgga
     7741 attggaagcc gaagaagatg ggtacttgat tgccattctc aaaggagatg gtgaaactgt
     7801 ccctgtaacg gaagttatcg gttaccttgg tgaagaaagg gaaaacatcc caacagctgg
     7861 agcagcatcg ccagaagcta gccctgtacc tgtagcaagt acctcaaacg atgatggtaa
     7921 gagcgatgat gcttttgata tcgttgtgat tggtggaggt cctgctggtt atgttgcagc
     7981 cattaaagct gcccaattcg gcggtaaggt tgcccttgtt gagaaatctg aacttggtgg
     8041 aacctgctta aaccgtggat gtattccaac caagacctac cttcataacg ctgaaattat
     8101 tgaaaatatc ggtcatgctg caaatcgtgg tatcgtgatt gaaaatccta acttcactgt
     8161 tgatatggaa aaacttttag aaactaaatc taaagttgtt aatactcttg ttggtggtgt
     8221 tgcaggactt cttcgtagct acggagttac tgttcataaa ggaattggta caatcactaa
     8281 agacaagaac gtcttggtaa atggttctga attgcttgaa accaagaaaa tcattcttgc
     8341 tggtggttca aaagtcagca agatcaacgt ccctggtatg gaatctccac ttgtcatgac
     8401 tagtgatgac attcttgaaa tgaacgaagt gccagaaagc cttgttatca tcggtggtgg
     8461 agttgtcggt atcgaactcg gtcaggcctt catgacattt ggttcaaaag tgactgttat
     8521 cgaaatgatg gaccgtatag ttccagctat ggatgtggaa gtttctaaga accttcgctt
     8581 gattcttgag cgtaaaggaa tgaccatctt gactggtact aaactgcaag aaatcattga
     8641 ggaaaatggt caacttcgta tcaaggttga aggaaaagac gatatcatcg caagcaaagc
     8701 tcttctttca attggtcgta tgccagacct tgaaggtatt ggagaggttg agtttgaatt
     8761 ggatcgtggt tgtatcaagg tcaacgaata catggaaact tcagttccag gcatttatgc
     8821 accaggtgac atcaacggta ctaagatgtt ggctcacgca gctttccgca tgggtgaagt
     8881 ttccgctgaa aatgcactta aaggaaatca tgcagttgcc aaattgaatt tgactcctgc
     8941 agccatctac actctccctg aagtagcagc agtaggtttg acagaagaac aagcccgtga
     9001 gaaatacgat gttgccatcg gtaagtttaa ctttgctgct aacggtcgtg ctattgcatc
     9061 tgacgcagct caaggtttcg taaaagttat cgctgataag aaatacggag aaatccttgg
     9121 tgtacacatt attggtcctg cagccgcaga attaataaac gaagcatcaa gcatcatcga
     9181 aatggaaatc actgttgagg aaatgctgaa gaccatccac gggcacccaa cttactctga
     9241 agtgatgtac gaagcgtttg cagatgttct aggaatggcc atccattcac ctaagaaaaa
     9301 ataaaacgaa gatagattag actggaaggt tatttccagt ttctatcata taaagtaaag
     9361 ggatatcatg aaatacatta tcaatcattc aaatgacact gcttttaata ttgccttgga
     9421 agaatacgcc tttaaacacc ttttggatga ggatcaaatc ttcctacttt ggattaacaa
     9481 gccatctatc attgttggtc gtcaccagaa cactatcgaa gaaatcaacc gtgattatgt
     9541 tcgcgaaaat ggtattgagg tagttcgccg tattagtggt ggtggagctg tttaccacga
     9601 tttaaataac ctcaactaca cgatcatctc caaagaagat gaaaataagg catttgactt
     9661 caagagcttc tcaactccgg ttatcaatac cctggctcaa cttggggtta aagctgagtt
     9721 cacaggtcgt aatgaccttg agattgatgg taagaagttc tgtggcaacg cccaagccta
     9781 tatcaacggc cgtatcatgc accacggttg cttgctcttt gacgttgatt tgtcagtcct
     9841 cgcaaatgcc cttaaggttt caaaagataa atttgaatca aaaggtgtga aatccgtccg
     9901 tgcccgtgta accaatatta tcaatgaatt accaaaaaaa atcacagtcg aaaaattccg
     9961 tgatttactc ttggaataca tgaaaaaaga gtacccagag atgactgaat acgtcttttc
    10021 agaagaagaa ttggccaaaa tcaatcacat caaggatact aagtttggaa cttgggactg
    10081 gaactatggt aaatcacctg aatttaacgt ccgtcgtgga accaaattca ctagtggtaa
    10141 ggttgaagtc tttgctaacg ttactgaatc aaaaatccaa gacatcaaga tttatggtga
    10201 cttctttggt attgaagacg ttgctgcagt agaagatgtc cttcgtgggg tgaaatacga
    10261 acgcgaagat gttcttaagg cgctaaaaac cattgatatc acacgctact tcgctggtat
    10321 tagccgtgaa gaaatcgctg aagcggtagt tggataaatc aaaaagaagt tggccaacta
    10381 accaacttct ttttttgata tttatttaca taatttaaaa tacgtaaaat tataaactat
    10441 ctagagcatt cttttgttca tcattaacaa tatgggtata gaggtcagtg acttgtgtgc
    10501 tggcatgtcc tagttggtga ctgactaaaa cttgtgattt agtcgcatca tagagcctag
    10561 ttgctagcgt atggcgcagt ttatggggtg ttacacgcac tttaaaatcc tctgagtatt
    10621 tagcaaccat tttctcaacg ctagaagcat cgatacgatt aggaacacct ctgtagagag
    10681 ttaaaaaaag ggctgtatct gttttttccg ttttatagcg ttgattccga atggccagat
    10741 aattctctaa ataaggttta gcaaaagcag cgacattgac tgagtcacgt ttgccacctt
    10801 ttcgagtaac atcaataacc atcattttta gattgagatc tcttagatct agattaacag
    10861 cttcagataa gcgaacacca gatgccaaga gaagggcaat aatggctaaa tctcgttctt
    10921 tatttttgtt gaatgatgag agagctcgat ttgaaagctg ttgtgggtgc tcttgatcga
    10981 tataagttag aaaaccttct gtttcatcac ctagaaagag tttttgcttg atattttcag
    11041 ctctggcagc aagggtttct ttctttttct tggttgaaac ttttttcatt acattacgat
    11101 agaaataagg ttccccctga tcgttttcaa cctcctcggt tagatacttg taaagactag
    11161 aaagtgctga taaggttcga ttgatagttg tctgtgaaac accctgtttt gttgtattag
    11221 cattcagcaa gggacgttca cgtagataaa ggataaagga ttccatgtct ttcttagaca
    11281 tattttccaa aactgataaa ggaatatcgg atattttatc agcgtttgaa ataccagact
    11341 ccaaaaccca gctgaaaaat cggtcatatt ccttaaggta ttcgtacagg gttgtaaaac
    11401 tgtagggtac agccagctta gattggtagt attccagaac ataccagggc atgagttgtt
    11461 ttagtttgtc gattcgttcc agtaaaatct cacgtttcat gtattttctc cataagtaag
    11521 tatactagta gtatagcata gacttgtata tttttcaaga aaattatagt ttttcagaaa
    11581 gatgttatag gagtttttca aagtgataaa aagacttaga cctacaaaca aaacagaata
    11641 aggaaaataa taaaaatctc acccactaaa cttctgttaa tactacatag ctagaaattg
    11701 atttgattgt cctgattgtt ttatcctatt tctatttcat ttcactatat aattgctatt
    11761 gttttctttt tcatgatttt aatcttactg tacagatttg atacagtaaa ctcctttcta
    11821 gctttatgct ttatcatttc taacttaatt atagtcttat ttttaataaa gttcaattat
    11881 ttatatgtaa aattcctcat cagtaaaaaa taaccgatct catttctgag aatcggtttt
    11941 ctaaataaat caagctgatc atttacataa cataaattat gtaaatgatt gtactacaac
    12001 aacaaatctt tgacttttcc aatttcactt tttggaataa ccaggtgaat catatcaccc
    12061 agatacattc tggttgagcc gttaactgtt tggctcttgc cattatggac ttgagttgtg
    12121 atgaggacgt tgtgtggtaa gttgagttca tgaacttgtt tcccagcaat tttatcagaa
    12181 actggtattt cgataagtgt aacttctcct tcgctagata cttcttctgg aagcattttt
    12241 tccagcatgg cttcatagac tggcgtacct ttgagcaaat ccatgataat ataagaaaca
    12301 agagtgacaa gaccaagtgg catgaggttg cgaatatctc ctaccatctc agttacgagg
    12361 atcatagcgg ttaagggagc ttttgatatg gctccaaaat agccactcat tcctagaatg
    12421 acaaatatag ggaattgctc ttgactgaca agtccaagat tgacacagat agcaccaatt
    12481 aaggcaccaa gcaaagaacc aagagctaaa atggggagga aaattcctcc tggcagtcca
    12541 cttccatagc taatcatact ccaaataaag cggatcaaaa agtaagctaa taaaacttgg
    12601 aaactaaaat tttgttcagt taaagaaagg acaagctgat ttccgccacc aatgatttga
    12661 ggtaagaaga ttccgactgg tatgataagg ataaaagcca agatgggata ataagctcta
    12721 tccaaatgga ttttttgacc aatcaagtca taaactcttc caacgtttaa tacagctttc
    12781 tcatagagaa agcctgaaaa tccaaggaaa attcccatga cgagatatat ccaatactga
    12841 tctagggtca tgggaggaat gttatctggc atatccaata ctggtgtcaa accgaacatg
    12901 agtagagaca caaagtttgc tacgatgctg gctgctagag ttgagaccca gaaaaagcgc
    12961 gaaaaatggt gatagacttc ttctacaaca aagagaagtg ctgcaatagg agcattaaag
    13021 gctgcggcta aacctgctgc agctccactg gcaatcaagg aacgttcctc tactggactg
    13081 gatttgagcc acttggcaat tcctttacca ccaactgctc caagttgaat actgggtccc
    13141 tctcgaccca gcatgagtcc actggcaata gcaagaatac ctagcacata ttttttccaa
    13201 agaatgcccc accagttgag ggacatgagg cctttcagtt cggcttcgac ttgaggaatt
    13261 cctgagcctt taatatcttt ttctgaccgt gttagtttgg cactgagcca acagatgagt
    13321 atataaaaca aaaccagtac aaaaagattg cgtactaagt acccttgatc ttgataaact
    13381 ccttgtatca ggtggaagcc cttttcaatt aagaaacgaa aggatccgac gataattcca
    13441 acgatgaggc cgacaatgat tcctcgacca acttgggata gtatagtgct ggatgcaaag
    13501 gcgaattctt tcttagaacg gactatttcc gactgttcct ccatggtatt tcctttcaat
    13561 taactttctt ttttacctaa aaccagtgat ttaacgggtt caaagctggt tcggtgaatt
    13621 ggggtaactc ctagttttgt gaggccttcc agatgtttag ctgtgccata tcctgcatta
    13681 gtagcgaaat catagccagg gaactgctga tcgtattcct tcatcaattc atcacgtgtt
    13741 accttggcta ctatagatgc tgctgcgata gagagggagt tggcatctcc tttgataatg
    13801 gaggtttgtg aaatgggcaa gtccagtttc atggcatcaa tcaaaaggtg ctctggttga
    13861 gggctgagct gggagattgc ttcttgcatg gctagtttgg ttgcttcata gatattgact
    13921 tggtcgatga cctgattatc tatgatacca attccaatcg acaaggcttg gtcttgaacg
    13981 gcttggaaaa tctccagatg tttcttttta ggaattttct tgctgtcgtt gagaccttta
    14041 atcttacaat ttttaggtaa aataacggcc gcagcgacta caggaccagc aagaggacca
    14101 cgaccaacct catcaatacc tgcaattaag gtcaatcctt gcttataaag ttctttttca
    14161 taagaaagca tggattccaa gcgcaaattt tcatctaatt cagcttgaat ggctctttta
    14221 cgcttgctga tttccttttg aactcctgag cgattatcct tttcaagatc taaaaaaata
    14281 gggctttcta actccttgac tgtcacaagg agttctttga tttctttaat cgttgccatc
    14341 gaggtcttcc aatgtatcta aggtatagtt accgagtttg ccatcacgga cttccttcac
    14401 gaagagactg taaaaacggt catagtcatc acggaaaccg agggcgcggg tcatatccat
    14461 aataatcaca ggcgcttctt cttcaatttt catttgtttg aagcgttcag ccagcttttc
    14521 tggataatgt tctttgaaat aattgatacc aaaaatggta acctcatcca taggaagcaa
    14581 ctggtctttg atagctccag tcaatgccaa cttaagtgca acagtttcat cctcaaactt
    14641 aggccagaga atccccggtg tatccaagat ttccagatct ttattggttt taagccattg
    14701 ttgacctttt gtgacccctg gcttgtttcc aacaacagca atctttttac cagccaaacg
    14761 gttcatcaga gttgatttac cagcgtttgg aatcccgata atcatggtac gcaaggtttc
    14821 aatctgaatc ccacgttctt tctggcgagc aatcttatca gccatgagct tcttggccgc
    14881 atctgttaca acttttacag tcacttgctc tttggagttg atagctagcg tctggattcc
    14941 ttgtgattca aaatactgac gccattcctt ggtcattgct ggatcagcca agtcggcctt
    15001 gtttaaaatc aagagttttg gtttatcacc aacaatcttg atcaacatag gattttgact
    15061 agatagaggt aagcgtgcat caactaaaat cgtcacaaaa tcaacaaatt ttaaattctc
    15121 ctgcacttgt cgacgagctt tagacatgtg accaggaaac cattgaatag tagccattga
    15181 gtttttttcc tttcgtagca aggatttaga tcccctattt tattttacta tagtttgaac
    15241 atagagagtt ttcaaatgaa tgatgtccaa atagttcttg ttttatgaca tagaaatcta
    15301 ctttatatta tatcatgcct tctttatttt gtccgatgat agccttttac cctatctttt
    15361 accctatata gaaaaaagcc atcaaatgat gacttttcag agggagcaaa aaactgttcc
    15421 cgtcatatta tgaaccaaaa gaactaaact accaacagct aataggccta aagctgtaag
    15481 cgcagaagtt gatgaacctg ttgcaggaag agtttcctgc tgataaataa gagcataact
    15541 tacttcatta tcaggaggaa ttcctgcttg aatcttggtt tgttcttcat caccttttta
    15601 aactttattt cctaatagta aacactattt tttattctca aaaattgaac ttctaaaatc
    15661 atttgtttgg tcaagatagg ctttaaagat tgctctcttc aacttatgga cggcactgtc
    15721 cgtttctgga ttataattac tccaacgtgt acctgtagca tcctgcaaaa cagcttcatc
    15781 cattagtttc tgcaattcat ccacattatt gatagtcttt gtgccatatc ttggccatgg
    15841 ttttgtcgga tctttaaagg tcacctgctt caagtttcca aactgatcca cccgttcttg
    15901 gtacatagct gtcttaaatt ctgcccaagt tttatactta ccatcaaata cctttttcaa
    15961 aacaagctca tcggtcacca agccccgttc tttaccataa agattgatag tttgcccttg
    16021 ttgtttagca tcttcttcgt attggttgga aatatatggt accataccat ctttaaatcc
    16081 tttagcagct agaagttcaa aagctatcct tcgtcccata aggtcacctg gagttccttt
    16141 ctcaccgctc aatgctgaaa agattggtgc aaagagtttt atagtaaagt agccatttct
    16201 ctcataagtg ccagccttat actcacgagc cgaaagaatg ttattgtcaa tcaaactatc
    16261 aaagctcgtc aattttttag cttcatcttc agtcaaattt ttcacaacat tagtagcata
    16321 aacatcattt ccatcagcag gatctgttag atattggttc tcgatttttc tgagagcttc
    16381 tattttctgg taaacactta gtttattaac gattgactgc ccttcgagat attccatcat
    16441 gtagataagg tcaaacatgt tatggacata gttctgaaga tctgctgcgt tttggaatct
    16501 ctctgtcgga tccaagactt gaagacggga tccctctgta ctatctgatt ttgagtgttt
    16561 caagatagaa ttgatggtaa tggttgcgtc acttggttgg tcaggggctt gcaataagcc
    16621 ttttgcgaag aactctggtc ccaagccact tcttcgacca tagccaccaa ggtaaatatc
    16681 ctgatccgaa tcatgggtca tttcatgggt ataagtaatg gctccatcct tgtccagcat
    16741 gcggtaagac atatagtaga cgccgtcccc tgtagcgtag gctccatgct ggttgtgtac
    16801 taccttattg ccaactggtc caaagaagtt cttcatagct ggattggagc tatcaaactt
    16861 agcctccgct gtagcttttc ctgatgttgt atcatcccca aacttatagg catcataaag
    16921 gagaatcgta cgatagagtt tttcgcgtga ctgttcatct aaaatacgat accaataatc
    16981 gtagtgatct cgctgacgtt tggctgtttc acgcgcattt tcttcaacaa aatcattgag
    17041 agccttgccc gctttatggt cactactgcg gtagcgatca taagctccaa atcctagact
    17101 agacatggtc gagatgacaa atacggatct ctctggcaag gtcaggagag gcaagaccat
    17161 attgcggtat ttccaagtat tactagtaat tcggtcataa acaccgatag agtacttagt
    17221 tcctgcttgc ttttgcttgg ctttgacctc tgcaatagtg gatttttctt cgacaatgta
    17281 agccttagtc tctgatttaa accagtcatt attgcttgta tttggtaaaa agacttttcg
    17341 gtaatgttcc agcgtgctaa acaaatctgt cgttccatgt tgactggcaa gactgatacc
    17401 ataagtatcg acattattct tagctagaag attgttaaag ccagatttac ccaactcaat
    17461 cagagtatct aatggtgaag cattcccctt accaaagaag tccaaatggt acagaactag
    17521 gtctttgaca ttcacctgac catagctaaa gttataccac cgttccagat aggtcaagcc
    17581 aagtagcaag gcttccttgt tgcgtttgat tttatctaca agataacctt cagtgactgg
    17641 gttagcacta gccagtccag catccgctga caagagtttt ttcaaactgt cttccagttg
    17701 ttgttttgtt ttggcgaact ggtcttctag atagagctca gtttgcttga cgtttggaga
    17761 aatacccagc gtctttctga tggcttctga atgatagtca accttttgta agtcaggtaa
    17821 gacttgcttg atgatagagg tttggtcata caggaattgg tttggcgtat agagaagtcc
    17881 agtattgccc agactatatt ctgctaattt ggcgaaatca ttctggtatt tgagatccag
    17941 cttctcagat aaatcatcct tgtagtgaag caagagtttg tttgcagtct gtttgttaga
    18001 aacaatgtct gtgatgactt ggttgtcctt catcatgact gctgacaaga gttctttttg
    18061 atataaaaga ctgttctcat tgaccaggtt tccgtatttg acgatggttg ccttgttgta
    18121 gaaaggtagc aatttttcaa tgtttttata agtcaagttg cgcttagctt gataataggc
    18181 caccttagaa aaatcactgt cttttttgcc acttgttgaa agtggctcca ctgttggtag
    18241 aatgaaagga ttgatttctg cttttttgct tgcaatttga gaagcatcta gcattgttcc
    18301 tctttcttca aaggattcct tgctgacgac ctcatccttg accaaggtga cattgtagac
    18361 tctgttggcc ttgctgctga atgtgtcctt taccttcatt tcgttatagt ggtaaccagt
    18421 gatggcattt ccgttggtta cattaacatc gctgagaaca ttggtcaaac ttccagcata
    18481 cctaacatca ccagaagttc gatcccacaa attgcctgcc actccagcga ctctaccaaa
    18541 gtgcttgaca ttgttgatat caccttcagc atagctatct tggatctgtg catctcggtc
    18601 tactaggcct gcaagtccac ccacagtctg atctgaagta tttgtgttag atgaaatggc
    18661 tactgtcgct tttgacttag taagtaaagc cttgtcacct gtcaaatgac cgaccatacc
    18721 accgatattg taggcagcag tcgtttcata agtgttgata attcttccct tgaaactgct
    18781 ctctgtgatg cttgattgct cagccttagc cagcaaacca ccgataccac gttcaccagc
    18841 cagaacacca tcgacgtgaa cttgcttaat ttttgtatta ttctgagctt catttgccag
    18901 tgaaccgata tcatctttcc ctgaaatagc aacatttttt agactcagtt tttctactgt
    18961 agcaccactc aagttttcaa acagaggttt tttcaaatta tagatagcat aattcttgcc
    19021 atctttttca ccgattaaac gaccagtaaa ggtgtccttg atataggatc tttcatcagg
    19081 accaagctcc acttcgttag cattcaggct ggccgctaaa tgataggttc cagagggatt
    19141 ttggtttata gctttgacca ggttgctaaa ggaagtaaag tttgtatttt cctctttagc
    19201 cttcttatcg aggtagaagg tgaaattgtc gacatattta ttttctagtt cttgctggag
    19261 tttctcagct ttggctgtga ttttataaac tgactgccca tttcttttct cttcagttat
    19321 tgaagctact ggtatataga catctttaaa tgctgaagat tttaccttga caaagtaagt
    19381 atccgtattc tctggaattc catccaatga aacatgttgt ctgtaggttc cattcgtctg
    19441 actatacaac tcaatatcag aaacatttct taattcgagt tttttctctg gttcttctac
    19501 ctttttatca gggtctagtt cattttcttg tttaatttct tcgtttccat ttgaattgga
    19561 tgtgtttgat tcggttgaaa catcctcagt tgaatttccg tttgatggtt ctggttctgt
    19621 ttgtccattc tctgatgttg tattacctga attttctggt tttgttgcag ttccgttttt
    19681 ttctggttga tttgattctt caactggtgg ttttgaatca ctaggtttat tggatacttc
    19741 tccagtattt tcgttagcta ttttcccaga gtttgtttgt gtttcttctg caggttgaac
    19801 tggtttttct gtttcttgat ttgaggtacc ttctactgtg ccttcatttg gatttactgg
    19861 aacttcttct acagtttttt ctgaattttc atttttagag tcattatgtt ctggtttatt
    19921 tgattctcca actgaggttg tcgaatcact aggattactg gacacttccc cagtattttc
    19981 gctagatgta tctggtgata ctttctctga attcgttgtt gattcttctg caggttgaac
    20041 tggattttct gcttcttgaa ttgaggttcc ttctgtagta ccttcatttg gatttactgg
    20101 tgtttcttct gttggtttta ctggaacttc ttcagttttt tctggacctt gttctttggt
    20161 cttctcaacc ggagtttcag gttttacttg ctcaatatta cccttatatt ctggcaatgg
    20221 ggctacctgc tctggttcac ctttatcact taccacagta tctggcgact ctggttgaac
    20281 ctcagtctca cctttgtcgg tcacaactgc ttcgggtaat gtcggttgaa cttctggttc
    20341 gcctttatca cttactacag cttcgggcaa ctcaggctga attgcgggtt caacaatagc
    20401 tccagactgt acgtccttat gttctacacc agtctcaggt tgttccttta taacttgagt
    20461 ttttttagta cctttttcga ctattcttgg actaggcgca gtcgttgaag ttgaaacaat
    20521 ttctcgcgaa acttcttcct tgtttacaga gaatattctg acgatttcaa ctttcttacc
    20581 taatttacct tcttgtttta ctcttacagt tccttcagct aaatcaggat tttcttgaat
    20641 ttcttcttga aaatctattt ttgtctctat agtttcctca cgatataaga gttcaggttt
    20701 gttcaattga cctgataaaa cttcatcctg tggatttaat gtatttaccc cagtcttttc
    20761 ctttggagaa atcttctcct ctttcttcgt ttctagattc ttatgttcgg ctaattgttc
    20821 ttgagaatct gaagattgtt tctcttcttt tcttggattg attaatttag tagagaaagg
    20881 tttttcaact acttgaactt ctgtcggctt agttgaagaa acaggtgttt gttcctgaat
    20941 agcttgtact gttgatggat ggtctacaaa atttggtgta acattataat ccaccttttg
    21001 ttgttttgta ggagtggctg ctgatttttc ttgattactt acttcaaaat cagaagtcgt
    21061 ttttccctct ttgatatatc caatataagt gtaacctgaa atctctttag gaagaggtaa
    21121 tttttctcca gaggtcaatt catagtccgt attgtaattt agcaaaagat gattttctaa
    21181 agcatggact gaaactaaga caccatttcc cattcccgca accaatacta aatgtaatac
    21241 cgttttattc ttaacctttt tcttggaaac agcaaaaatt aaaattccca gagcagctaa
    21301 gctagcacca gcaactaggg cttgcctctc attcttgctt ccagtatttg gtaattccac
    21361 cagttgattt tgagaattta acttataaac aagataataa gtttcatcat cattctccac
    21421 gtatgtcgga atatcataga caagctgctt cttttcttct gatgatagct ctgaatctgc
    21481 cacatattta tagtgaactc ccgcagtttc ttgagcatcc acagatgaac tagctaatac
    21541 agacataaaa aataaacttg aaatcgttgc agatacaagt cctactgata attttctaaa
    21601 tgaaaaacgc tcttgttttt caccaaaata cttttccatt attcctcctt gaaataaaat
    21661 ttatatatgt tacaaagacc tttattatat tagtgtatta tctattatct atagaaaagg
    21721 cagtatacct taattatact cttaatttac aaaaaagtct taaaattgag atgcgctttc
    21781 atactttgtt ttatattatt tgggggtaca ataacaccta ccatgaaatt tacacggtag
    21841 gtgttactca tatcactaat cgttctaaaa atggtttgag gcagttgagg agaattcctt
    21901 ctatccagct tccttgtgct gatgagcgat ggtcttcctg caggcttttt tttagaaaat
    21961 ctcggacttg ttctggtgcg atttcaaatt caaaggcttt cattttatag aaaaagtcga
    22021 tgagatgatc ggacaggtat tcagttgaaa agggtacttc accacttttt cgatattcta
    22081 ataagagcct agaaaatcga gctttttctt caggaagctc acgaaaatag gaattgagga
    22141 gccaagtctg cttctgtttt ctttcaattg gatcctgact ggcaattcgt tggtcttttt
    22201 ccagctcttt ttggtattgt ttggccttga tagcccgttc tgctctattt ttaccaaaaa
    22261 gaattttttc ccacttgcgt tcttcttgag tcagggtctc tgtaaagcca aagtaatctt
    22321 gataagcacg ctctgcgggt cccatggcta gaaccagatt gtctgcatat tgcttggcga
    22381 ttttatccct cttcttgcgt tctttctctg cctggatacg gagttcttgt tcgtagtcaa
    22441 ttttctcctt gcctagcttg acaaggtaga gttggtcatc cgatttccca agtaaaaagg
    22501 gtttgataca cttttcaagg acttcttcca tccgagcctt tttctttggt tccgccttgg
    22561 tccaacttcc tccctgaaag acttctagga aaagctggta gtctctctca ggcgcaaatt
    22621 gattgccacg attgggtttg aaaacacctt tttcccagag ccattttaga agtcgctcgt
    22681 caaagtcact tttattgacc ttgatttttt cctttttctg agcttttctg gttagatttt
    22741 caacctttct gagcagtttt tcttcctctt ccaattgctg gtcaagggac aatcgatgaa
    22801 aatgacgaac acagtcgcta ccaattggaa agaggcgttg gcctgtgaca ccgttaaaga
    22861 gttcataagc gtatttgatg gcatttccac agacacaatt gctacggccg ataccgttaa
    22921 aaatcaagga aacttcattc cattccttgg tagcttgttc ccaagtatcc gctttcgaag
    22981 cctgtaaaac tgcatcgtgc agggattttc taactggaag tgtcatgagg tctcctttct
    23041 aatactcaat aaaaatcaaa gagcaaacta gaaagctagc cgcaatcagc tcaaaacact
    23101 gttttgaggt tgtagataga actgacgaag tcagctcaaa acactgtttt gaggttgtgg
    23161 atagaactga cgaagtcagt aaccatatat acagcaaggc gaagctgacg tggtttgaag
    23221 agattttcaa agagtataag ttatactttt acaacttgaa cctcgtcttt accgagtaaa
    23281 atcaagtatt tttcaatatt ttcaatcgaa taggctcgtg ataaagcctc ttcgtataga
    23341 gctaactgac cacgatagcg gtctacgagt tgacttggtt catcatagcg gtctgtcttg
    23401 tagtcgaaca gaacaatttt gttttcgtaa agcagatagc catcaaggat accacggaca
    23461 acaaagtctt cctgactctt ttggtctcgt ttgagcatgg agaaaggttg ctcgcgatag
    23521 agatggtcgg tattagcaag aatttcctga ccgagtactg tgtcaaagaa agcaagaatt
    23581 ttatcaagat tgatcttgtc tctgacagct tggctagttt gaacttgttt gagtgtttct
    23641 gttaggctag caagggttag ttgctggctg aggtcaattc tctgcatgag ttcgtgagta
    23701 gcactaccaa tctcagctcc agttaccttt tctttggttg aaaaatctgg caaatcgaag
    23761 ctgattttct tgcctactga ctgaccttga ccagcaatct cgacaccttc catatccata
    23821 actggttcgt agaatttctt gatttgactt ggggtttgaa cactaggaag ttcaatagct
    23881 gcgcggtgaa gagtattata aacttccacc tccttcagca tttccagagc ttctttgatg
    23941 gtatctgact gacgattgtc tgcttgggag ctatcttgga gaggactctt ggtttccaac
    24001 tctccgatag cttctctggt caactgatct tcgccaataa aacgataact aaagttgagc
    24061 ttgtccttag taaacacttt actgatagcc caaagccaat cttggaaatt ccgtgcttgc
    24121 agtctagtat tgctatttag tttcccattt ttggctgctg ggtattgctt gacttccagc
    24181 ttttcacgag aacccttgcc gacaagatag agctttttct cagcccgcgt catagcaaca
    24241 tacagcaaac gcatctgctc agaatagctt gctagctgta attcctcttc gttctgccta
    24301 taggtcagac taggaatgga gagtttgatg gttttaggat agtggtcttc tactgcccct
    24361 gtctccatct tggcaatata tttgacacca agaccattct gacgactgag aatgacttct
    24421 gacatagagt cttgcttgtt gaaatcttga tccatattga ggataaagac gtaaggaaac
    24481 tccagccctt tactcttgtg gatggtcatg agctctactg catcttttgg cggtgcgacg
    24541 gccacgcttg ccaaatcgtg ctgggcttct aagacttggt caatcatacg aataaaacgc
    24601 gacaaacctt tgaaattgct cttttcaaat tgatcagcac gcagtgctag ggcatagaga
    24661 ttggcctgcc tagcaggacc attcggcaaa gccccaacat agtcataata aaaacggtcg
    24721 ttgtaaatct tccaaatcaa gtcatagaga gagtgggttt tggcatacaa gcgccaagaa
    24781 gctaggatat ccatgaattg ctttagtttt tcagctagag ctgtgtgaat caagcctttt
    24841 tgactacttg ccattttttg tgcattgacc agtttctcat agagattttc gtggacttta
    24901 tcctctgctt tctgaaggga caaacgtgct agctcatcct catcaaaacc aaacattgga
    24961 gacttcataa gggcaaccaa ggcatagtct tgcaggggat tgtgaatgac acgaagagtg
    25021 tctagcatga cttgcacttc tagggattgg agataattgt tttgctctcc gtcagttttg
    25081 acaggaatcc cgtactcaga cagggcgagg agaatctggt cattacgact gcggctggag
    25141 gtcagaaggg caatttcctt aaaggtaaca cctttttctt gatgaagttt cagaatctcc
    25201 ttgataacta agcgcatttc gcctgttagt ttcgtttctg tttgactctc ttcttcctca
    25261 cctgtatcgt ccttgtcgta gaggagaaat tctgccttgt tgtctggatt gggagtcagt
    25321 ttggtattgg caaaaacaag ctggtgcttg ttatcatagt tgatttcgcc gacctcttgg
    25381 tccatgagac gttcaaagac atcattggtt gctgacagca cttctgaact actacggaaa
    25441 ttttccttga ggagaatgag cttgccttct tggggatttt gcgcatagcg ttggaatttc
    25501 tcattgaaaa tctgcgggtc tgcctgacgg aaacgataga tggattgctt gatatctccc
    25561 accataaagc gattgtggcc attagacaac aattccagca tccgttcttg aatatggttg
    25621 gtatcctgat actcatcgac catgacttca tggaagcgct cctgataaga ctcacgaact
    25681 tgtgggaaat tttctaaaat ctcaatggtg taatggctga tatcagcgaa ttcaaaggca
    25741 ttttcctgtc gttttctctg acgataagcc tctacaaaat cgctcatgaa agattggaag
    25801 gttttagcta gtttccaagt gtctccatga taacgttctt gatagtcgag aatcgctatc
    25861 tggtctgata attgtcctag tttagtaaac tgggtctttc tctcttcgtt gtaggcatca
    25921 gccaggggct tcaaatcagc cttacggctg gcattagtca gagcacgacc gtttttctcc
    25981 ttagagatgg cgacaacacg cgcaagtact gcctgataag cctgactatc gaactcctga
    26041 tttagggagc caatttcatc cagaattaac tgaacatttt ctaaataggc agcctttgca
    26101 aactccttgg catcgttatc cagatggtaa cggaaaaagc tttccaaatc ccagagggct
    26161 tgtttgattt gctcagtcag tttttctttt tcactggtaa aatcagcttt ctcaaagcct
    26221 ttgaggaaag attcactcag ccacttttga ggattactgg tggattggag gaagtcatag
    26281 attttataga cttgctgccg cagaccccgt tcgtccttgc cacgcccagc aaagtttttc
    26341 agcaaatgac taaagctctc tttctgttta ccttggtaat gcgcttcaaa gacctcatga
    26401 aagacttcgt tttcgagaag aagttgctcg ctttggtttt gtaaaatacg gaaattaggt
    26461 gcaatatcaa gcagattacc atgtttgcca aggaattttt gtgtgaaaga gtccatggtt
    26521 ccgatggcag cgttgggtag gtctgccaac tggcgaccca agtgttgttt gagatcgaca
    26581 tcatctgttt cttggatttt cttgctgatt tttttctcta aacgttcttt aagttcagtt
    26641 gcagccttga cggtaaaggt tgagataaag agttgagaaa tttcgacacc acgcgccaat
    26701 tggtccagaa tgcgctctac catgacaaag gtctttccag aaccagccga tgctgagacc
    26761 aggatattct gaccagaagt gtagatagct tcgatttgtt cggcagtttt cttctgttcc
    26821 ttgctcgaat ttgcttctgc ttcttgcagt ttttgaatct cctcctcact taaaaaggga
    26881 ataagcttca tcgatttaac tcctctctta ttttttcaaa ccaagcttgc ttgagttttt
    26941 ctccgaccag acgcttgcca tcagctaggt ccaatttttc taggaaacgg gcttggccca
    27001 gatggtaatt ggcttcaaag cctgtaatag cctgatgttg ctggacgtat ggggcaatgc
    27061 ttctgccatt ttcagtataa ggattgatgg cgaaccggcc tgctaaaatc ttctcagcag
    27121 ctttcttgta aagataggca ttgtagtcca gtaggagctg aaattcctca tctgtcagtt
    27181 gattagcctt gtttttgtta taaaattcgc ctaaataact gctttctttt tccaagaaga
    27241 gcccttggta tttcatagat ttgctggctt ctaccactgc tcctgccaga ctttttaccg
    27301 ccatcagaga ttggacaggt tcagccattt ccaagtacat ggcgccgaaa aagttctgct
    27361 ccccttctct ttttagggca gcaagatagg ttggtaactg agaattgagc ccattaaaga
    27421 aatgaggaaa ctggaactga gtcagactgg atttgtagtc tactactcct atcgctccat
    27481 tagctttcaa acggtcaatc cggtccacct tgcctcgtac aaagacactg cgtccattgt
    27541 ctaattgaat aaaggcttgg tcttttccac caaaatttgc ttcttctttg atggtttcga
    27601 tggctggatt gtgtcggaga atatgtccag tcgtccgtgc aacatcaagc aaaacttcct
    27661 tggtaaactg ggcttccaaa ctttcttgat aaatagcttc aaattcgcgt tcttgactgg
    27721 tttcttgaat agcttgttct agacgttggt caaaggaatc ttcattaggc aactgtaagg
    27781 cgcgttcaaa gatacgatgc aagaaattcc cgtgactacg ggcatcagga cgcaaacgta
    27841 attcctcctg caagcctaaa acgtagcgta ggaaataact gtattcattg cgataaaact
    27901 ctgtcaaacc cgacgtagac aggtaaaact cctgtttggc aggatagaga gcttgcaagg
    27961 tgtccttggc taaggtcttg ctgcttggac tggttgggat agctggattt tccagacctt
    28021 gcagatctag ttttttacct atgacacgcg acagaacctt gacaaaagtc aaatctcgct
    28081 caatatcgct catctcaccc tgctggtgat aggcaaccag actagacaaa agactgtgat
    28141 aggaccccat atcctcctta gacagtcctt tgtgattcat cctcttctct ctccgcctaa
    28201 atccaaaatg gatcaactct tgaagatagg cagattcctt actttcactt tcgttaaaaa
    28261 ggcttggagc cgacaagaac aactgcttac gagcagaatt gaccaaggaa agcatagtgt
    28321 agcgattttt cttgagattt tcactgctgg caatcagtaa ttgaacgcct tcttcggtcg
    28381 tttggtttag gttttgcctt tcttcatctg tcagaagact ggtgttttga gaaatttttg
    28441 gtaaattgtc ctgagttagt ccaatagcat agacaaagtc agcagtcaat ggtgcaatca
    28501 aatcgtaact ctgcaccaga acagtgtcca ctgttgctgg aatggtacgg tattgggaca
    28561 aactcattcc agaatggagc aaggctagga agtcttccag actaacctgt gaaccagcaa
    28621 aaacagtcgc aaattgttct aaaacatggc agaaagcctt ccaaacttcg gcttgtcttt
    28681 cctgttctac agcttccaaa gtggttgtca aatcttgtaa ctgcttggtc acagctcctt
    28741 cttttagaaa gacactccat ttttgtagga ggttttcagc cttttgtttt cggctggcaa
    28801 agagggtttc aagaggtgct aaaattctca ggcggaggac attcaaacgc tcaagattaa
    28861 attttccatg gtgggatttg gtgaaggttt gctgaaaggc tggcaagcca ttgataccaa
    28921 gatagcggat atattgctca aaagaatcaa tatcagactg actgaggtca gtatacaaat
    28981 cagttctaag aagattaatc aaatcctcct gacgaaaacg gtaacgtttt aaagctaaaa
    29041 tagactcgac aaactgagtc aagggatgat gagccatggc ttcgcttcta ccaagataaa
    29101 aaggaatctg atactggtca aaaatggttt tgagagataa ctggtaagaa gctacatccc
    29161 ccaagagaat acgaaaatgc ttgtagctca ggtctgagtt ctcatgtaat ttctgacgaa
    29221 tgctacgggc tactagctcc aactcctcct tttgcgtcaa acaagaccag atttgtaaat
    29281 tttcacggtc tttctcatcg atatccaaag cgagttctga aaagtcataa gaagactcca
    29341 acaaacgaga ggccttgtca aaactatcca tcttctcatg agtttgagaa cagtcctgag
    29401 caggcgtttg gtatttagaa gccagatgat ggagaaattt tacgctggct tggtagagat
    29461 tgccctcgct aaaaggactg gtataggctt tcttactagc ataagccccg ataacaatct
    29521 caacaccttt gccgtgaagt aagtccacaa cccgctcttc ctcagcagaa aaacgagtaa
    29581 agccgtcaat gaccaaggtg atttgattaa aatcactact taccttgtca ttctcaatag
    29641 cctcaatcaa atgggacaac tgacttccct gggctaactg cccttgatta agataggctg
    29701 ttactttctc aaaaatcaag agtaaatccg tcctcttatc ctcatctgtt aaattctcca
    29761 agtccaaaaa actcatctga gatttggtca tctcatggta aagctcaatt aactgctgga
    29821 tcaattgagg atcctgctta atagcgccat aaacacgcaa gtccttggga tcgagttcgg
    29881 caaggcattt gtaaaaggcc aacccaagac cgatatcatc aagagtagtt ttaactggta
    29941 aatcattcaa gaccagataa cgagccattt gagcaaagcg cgtgacggta atcgaaaaag
    30001 aagcctgctg ggacaagcat tccagcacgg cgcgttcctt ttcaaaagaa agagagttgg
    30061 gggcaatgta gaagacccgc ttgccagctg caactagctc ttctgcctct cttgttagaa
    30121 tttctgtcaa agaagttcga atatcagtat aaagtaattt catctcagcc tcgttggaat
    30181 ttttcatcac cctatattat accatgatta gcctcgtaaa tctgttaaaa tatttaggcc
    30241 atcctttctt ttcttcatca tctgctaaat cttaaatact tagctttact tgtattagat
    30301 agaataagtc tggctactga aaatcacata ataaaaaagc ctcggtaaca agactttgag
    30361 ttttatgatt gtttcttagg tacggaatac acttcaatgt gttgtcccag tatcttaatg
    30421 tcgacgggta gattgtctga tttatcgcca tcaacatcgg actctaattc gatatcagaa
    30481 gaagttttaa tattacgtgc ctttatatat tcaatattct tgatagaatg attgaactat
    30541 agtaaattga aactataata gtacaccgtg tatgctaaaa tattgctaga aattaatttg
    30601 atttccctaa tcaagctgtt cgtatcttat ttcaatctac tatagtaaaa tgaa
//
LOCUS       DAVHIT010000019        30437 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVHIT010000019 DAVHIT010000000
VERSION     DAVHIT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.019"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            5..75
                     /locus_tag="VVN16_000697"
     tRNA            5..75
                     /locus_tag="VVN16_000697"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VVN16_000698"
     tRNA            110..183
                     /locus_tag="VVN16_000698"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            267..356
                     /locus_tag="VVN16_000699"
     tRNA            267..356
                     /locus_tag="VVN16_000699"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:303..305,aa:Ser,seq:tga)
     gene            367..440
                     /locus_tag="VVN16_000700"
     tRNA            367..440
                     /locus_tag="VVN16_000700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:401..403,aa:Met,seq:cat)
     gene            444..516
                     /locus_tag="VVN16_000701"
     tRNA            444..516
                     /locus_tag="VVN16_000701"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:477..479,aa:Phe,seq:gaa)
     gene            529..609
                     /locus_tag="VVN16_000702"
     tRNA            529..609
                     /locus_tag="VVN16_000702"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:563..565,aa:Tyr,seq:gta)
     gene            614..684
                     /locus_tag="VVN16_000703"
     tRNA            614..684
                     /locus_tag="VVN16_000703"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:646..648,aa:Trp,seq:cca)
     gene            699..771
                     /locus_tag="VVN16_000704"
     tRNA            699..771
                     /locus_tag="VVN16_000704"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:732..734,aa:His,seq:gtg)
     gene            782..853
                     /locus_tag="VVN16_000705"
     tRNA            782..853
                     /locus_tag="VVN16_000705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:814..816,aa:Gln,seq:ttg)
     gene            863..946
                     /locus_tag="VVN16_000706"
     tRNA            863..946
                     /locus_tag="VVN16_000706"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Leu,seq:caa)
     gene            1053..2330
                     /locus_tag="VVN16_000707"
     CDS             1053..2330
                     /locus_tag="VVN16_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU5572137.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2406..3890
                     /locus_tag="VVN16_000708"
     CDS             2406..3890
                     /locus_tag="VVN16_000708"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5572138.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFASANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDAEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQVAVEAYLGL"
     gene            3951..5231
                     /locus_tag="VVN16_000709"
     CDS             3951..5231
                     /locus_tag="VVN16_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5572139.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAIWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5235..5627
                     /locus_tag="VVN16_000710"
     CDS             5235..5627
                     /locus_tag="VVN16_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5572140.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIQQFIYTHKGNNAFTILKDL
                     GVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLDPEKTYYIGDRTLDVEFAKNSGIQ
                     SINFLESSFEGNHRIQALADIPHIFESK"
     gene            5804..6946
                     /locus_tag="VVN16_000711"
     CDS             5804..6946
                     /locus_tag="VVN16_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5572141.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVFWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPPAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7266..7691
                     /locus_tag="VVN16_000712"
     CDS             7266..7691
                     /locus_tag="VVN16_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU5572142.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7773..8078
                     /locus_tag="VVN16_000713"
     CDS             7773..8078
                     /locus_tag="VVN16_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU5572143.1"
                     /translation="MESIATQSDATSRVFNMEKEELAHPSVSSLFKNQGIYKALLGVF
                     LLYGIYFSHNLEIVTIFVLFVIGAATYGSLTADKKIILKQGGSAILALISILLFKYT"
     gene            8207..8803
                     /locus_tag="VVN16_000714"
     CDS             8207..8803
                     /locus_tag="VVN16_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU5572144.1"
                     /translation="MSPFEAIKGLPAEIHGAEVRWLEVPTVFHKSAQVLEEEMNRYQP
                     DFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPIDRPIRPDGASAYFSSLPIKA
                     MVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFSYVKAGFMHIPYMMEQVVNR
                     PTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(8831..9688)
                     /locus_tag="VVN16_000715"
     CDS             complement(8831..9688)
                     /locus_tag="VVN16_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU5572145.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9818..10960
                     /gene="tgt"
                     /locus_tag="VVN16_000716"
     CDS             9818..10960
                     /gene="tgt"
                     /locus_tag="VVN16_000716"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5572146.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11061..12878
                     /locus_tag="VVN16_000717"
     CDS             11061..12878
                     /locus_tag="VVN16_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU5572147.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13031..14182
                     /gene="nagA"
                     /locus_tag="VVN16_000718"
     CDS             13031..14182
                     /gene="nagA"
                     /locus_tag="VVN16_000718"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU5572148.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14345..15403
                     /locus_tag="VVN16_000719"
     CDS             14345..15403
                     /locus_tag="VVN16_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU5572149.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15554..15919
                     /locus_tag="VVN16_000720"
     CDS             15554..15919
                     /locus_tag="VVN16_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU5572150.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15995..16936
                     /locus_tag="VVN16_000721"
     CDS             15995..16936
                     /locus_tag="VVN16_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU5572151.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16884..17900
                     /locus_tag="VVN16_000722"
     CDS             16884..17900
                     /locus_tag="VVN16_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5572152.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVRTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17902..18228
                     /gene="comGC"
                     /locus_tag="VVN16_000723"
     CDS             17902..18228
                     /gene="comGC"
                     /locus_tag="VVN16_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU5572153.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKEDGRITEEQAKAYKEYNDKNVGA
                     NRKVYD"
     gene            18221..18625
                     /locus_tag="VVN16_000724"
     CDS             18221..18625
                     /locus_tag="VVN16_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU5572154.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQMISNGGQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18630..18890
                     /locus_tag="VVN16_000725"
     CDS             18630..18890
                     /locus_tag="VVN16_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572155.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18853..19314
                     /locus_tag="VVN16_000726"
     CDS             18853..19314
                     /locus_tag="VVN16_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU5572156.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19292..19705
                     /locus_tag="VVN16_000727"
     CDS             19292..19705
                     /locus_tag="VVN16_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU5572157.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            19842..20429
                     /locus_tag="VVN16_000728"
                     /pseudo
     CDS             19842..20429
                     /locus_tag="VVN16_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            20490..21443
                     /locus_tag="VVN16_000729"
     CDS             20490..21443
                     /locus_tag="VVN16_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5572158.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21494..22684
                     /locus_tag="VVN16_000730"
     CDS             21494..22684
                     /locus_tag="VVN16_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU5572159.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSIPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22833..23204
                     /gene="rnpA"
                     /locus_tag="VVN16_000731"
     CDS             22833..23204
                     /gene="rnpA"
                     /locus_tag="VVN16_000731"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5572160.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYQEGNGSEKETKVD"
     gene            23179..24003
                     /locus_tag="VVN16_000732"
     CDS             23179..24003
                     /locus_tag="VVN16_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5572161.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNEVTSDITADSADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24022..25008
                     /locus_tag="VVN16_000733"
     CDS             24022..25008
                     /locus_tag="VVN16_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU5572162.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25059..25682
                     /locus_tag="VVN16_000734"
     CDS             25059..25682
                     /locus_tag="VVN16_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU5572163.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKVFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25813..27270
                     /locus_tag="VVN16_000735"
     CDS             25813..27270
                     /locus_tag="VVN16_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU5572164.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27293..27574
                     /locus_tag="VVN16_000736"
     CDS             27293..27574
                     /locus_tag="VVN16_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5572165.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27594..28079
                     /locus_tag="VVN16_000737"
     CDS             27594..28079
                     /locus_tag="VVN16_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5572166.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28096..28761
                     /locus_tag="VVN16_000738"
     CDS             28096..28761
                     /locus_tag="VVN16_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU5572167.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28765..29628
                     /locus_tag="VVN16_000739"
     CDS             28765..29628
                     /locus_tag="VVN16_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU5572168.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIHETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            29630..30334
                     /locus_tag="VVN16_000740"
     CDS             29630..30334
                     /locus_tag="VVN16_000740"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU5572169.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
BASE COUNT         9070 a         5417 c         7002 g         8948 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaaac tattttggag gattacccaa gtccggctga agggaacggt
      301 cttgaaaacc gtcaggcgtg taaaagcgtg cgtgggttcg aatcccacat cctcctttta
      361 tattaacgcg ggatggagca gctcggtagc tcgtcgggct cataacccga aggtcgtagg
      421 ttcaaatcct gctcccgcaa taaggctcgg tagctcagtt ggtagagcaa tggattgaag
      481 ctccatgtgt cggcggttcg attccgtctc gcgccattta tatattttgg aagggtagcg
      541 aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg gttcgggggt tcgaatccct
      601 ccccttccat tttacgggca tagtttaaag gtagaactaa ggtctccaaa accttcagtg
      661 tgggttcaat tcctactgcc cgtgttaata gaattatggc gggtgtggtg aagtggttaa
      721 cacaccagat tgtggctctg gcatgcgtgg gttcgatccc catcactcgc ctattttata
      781 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      841 ccagctaccc cagttactat ttgccggcgt ggcggaattg gcagacgcgc tggactcaaa
      901 atccagtgtc cgcaaggacg tgccggttcg accccggccg ccggtatagt atagtgttag
      961 gaacgttgtt attcttcgtt ccttttttat attatttttg gtataattat agttattcaa
     1021 attttattta gattaagaaa gtgtagggga gtatgtcttg ttctatcgat ttattaaaac
     1081 atcggtattt gaaaaatatt aaagaaaatc ctgaattgtt tgtcggaatt gagttggagt
     1141 atcctgttgc aagtttagaa ggggatgcta cagatgttga agttataaag gatctatttc
     1201 attatttagt ttctactttg gatctcaccg tagcaaaggt agatgatttt ggcaatctga
     1261 tccagttagt agatccgata agtcaggatg ctattttatt tgaagtttcc tatacaacga
     1321 ttgagtttgc atttggtaag gctgaaacga ttcaagaggt cgaaaatcgt ttcaataatt
     1381 atatgaatgt aattcagaga aagttagctg aatcaaatca tgctattgtt ggctgtggta
     1441 tccatcccaa ctgggataaa aatgagaatt gtccagtggc ttatccacgc tatcagatgt
     1501 tgatggatta tttgaatttg agtagaaata ttattaaatc agatttacat catttccctg
     1561 aatatggtac ttttatctgt gggagccagg ttcagctgga tatttcaaaa accaactact
     1621 tacgggtgat taatgctttt actcaaattg aagcggctaa ggcttattta tttgcaaact
     1681 ctgagttttc gggtgcggat tgggatacga aaatttcaag ggatattttc tgggaagaat
     1741 ctatgcatgg tatctatcca gagaatgttg gggtcaatgc tagactcctt aatgatgaag
     1801 ctgatttttt tgactatcta aatcattctg cgatttttac tgcggaacgt gatgggcaga
     1861 cctattattt ttatcctatt caggctgggg actatttggc tacgcctgaa atccaagcat
     1921 ttgctctgaa tggggatgag gttattattt ccccccaaga gaaggatttt gaaactcatc
     1981 gtagttacca gtaccaagac ttaacgactc gaggaacagt tgagtttcgt agtgtgtgta
     2041 cacagccact tgataggact tttgcttctg cagcttttca cttgggatta ttggttaatt
     2101 tagacaagtt agaagcttac ttagaaacag cacctttctt taaagtattt ggttatgatt
     2161 acaagtcttt aaggagacaa ttttctaaga aaaatcttac agatgaggaa gaaactacga
     2221 ttattgaatt ttccaaagac ttactcctac tagctgagga gggactagtg gtgagaaata
     2281 aggaagaaat gacctattta cagcctttga gagaagaatt gagcctataa tttctcttat
     2341 aaagggagaa ttttctgaaa aatcatgata taatggacga gactatagat aaaggataga
     2401 gagtaatgac attagtttat caatcaacgc gtgatgccaa caatacagta actgccagcc
     2461 aagcaatttt gcaaggtttg gcgacggacg gcggtttgtt tacaccggtt acttatccaa
     2521 aggtagattt gaactttgac aaattgaaag atgcttctta ccaggaagtt gctaagctag
     2581 ttttgtcagc atttttagat gactttacag ttgaggagtt ggactactgt atcaacaatg
     2641 cctacgatag caaatttgat actccagcta ttgcaccatt agtgaaatta gatgggcaat
     2701 acaatttgga acttttccat ggttcaacga ttgcctttaa ggatatggcc ttgtctattt
     2761 tgccatactt tatgacgact gctgctaaga aacatggttt ggagaacaag attgttatct
     2821 tgacagcgac atctggtgac acggggaaag ctgctatggc ggggtttgcg gatgtgcctg
     2881 gtactgagat tatcgtcttt tatccaaagg atggtgtcag caagattcaa gagttacaaa
     2941 tgaccactca aactggcgac aatactcatg ttattgctat tgatggtaac tttgacgatg
     3001 cgcaaacaaa tgtgaagcac atgtttaacg acgtggctct tcgtgaaaaa ttgactacca
     3061 acaagttgca atttgcatca gctaactcta tgaacattgg tcgtctggtg ccacaaattg
     3121 tttattatgt ttatgcttac gctcaattgg tcaagactgg tgagatcgtg gctggtgata
     3181 agattaactt cacagtacca acaggaaact ttggaaatat cttggctgcc ttttatgcca
     3241 aacaaatcgg tctcccagtt ggtaaattga tctgtgcttc aaatgacaac aatgttttga
     3301 cagacttctt caagacacgt gtttacgaca agaaacgtga gtttaaggta acaaccagcc
     3361 catctatgga tatcttggta tcttcaaact tggagcgttt gattttccat cttttgggga
     3421 atgatgcggt taagacggct gaactcatga atgccttgaa tacacaagga caatatgaac
     3481 tgactgattt tgatgcagag attctggagc tctttgcagc tgaatatgcg actgaggaag
     3541 aaacagcggc agaaatcaag cgtgtttatg agttagattc ttatattgag gatccacata
     3601 cagctgttgc ctcagcagtt tatagaaaat accaagcggt tactgatgat gcgactaaga
     3661 cagtgattgc ttcaacagct agtccctaca agttcccagt agttgcagta gaagctgtaa
     3721 ctggaaaagc aggtttaaca gactttgaag ctttggctca attacatgaa atctcaggcg
     3781 ttgcagtgcc accagcagtt gatgggcttg agacagctcc tgttcgtcac aagacaacag
     3841 tggcagctgc tgacatgcaa gtagcggttg aggcttattt aggactttaa gatagaggga
     3901 gtaaactcgg ttgggaaacc aactgagttt cttttcatca ggaggagaga ttgtttaaga
     3961 aaaataaaga cattcttaat attgcattgc cagctatggg tgaaaacttt ttgcagatgc
     4021 tcatgggaat ggtggacagt tatttggttg ctcatttagg attgatagct atttcagggg
     4081 tttcggtagc tggtaatatt atcaccattt atcaggcgat tttcatcgct ctgggagctg
     4141 ctatttccag tgttatttca aaaagcatag ggcagaaaga ccagtcgaag ttggcctatc
     4201 atgtgactga ggcgttgaag attaccttac tattaagttt ccttttagga tttttgtcca
     4261 tcttcgctgg gaaagagatg ataggacttt tggggacgga gagggatgta gctgagagtg
     4321 gtggactgta tctatctttg gtaggcggat cgattgttct cttaggttta atgactagtc
     4381 tgggagcctt gattcgtgca acgcataatc cacgtctgcc tctctatgtt agttttttat
     4441 ccaatgcctt gaatattctt ttttcaagtc tagctatttt tgttctggat atggggatag
     4501 ctggtgttgc ttgggggaca attctgtctc gtttggttgg tcttgtgatt ttgtggtcac
     4561 aattaaaact gccttatggg aagccaactt ttggtttaga taaggaactg ttgaccttgg
     4621 ctttaccagc agctggagag cgacttatga tgagggctgg agatgtagtg atcattgcct
     4681 tggtcgtttc ttttgggacg gaggcagttg ctgggaatgc aatcggagaa gtcttgaccc
     4741 agtttaacta tatgcctgcc tttggcgtcg ctacggcaac ggtcatgctg ttggcccgag
     4801 cagttggaga ggatgattgg aaaagagtcg ctagtttgag taaacaaacc ttttggcttt
     4861 ctctgttcct catgttgccc ctgtccttta gtatatatgt cttgggtgta ccattaactc
     4921 atctctatac gactgattct ctagcggtgg aggctagtgt tctagtgaca ctgttttcac
     4981 tacttggaac ccctatgacg acaggaacag tcatctatac ggcaatctgg cagggattag
     5041 gaaatgcacg cctccctttt tatgcgacaa gtataggcat gtggtgtatc cgcattggaa
     5101 caggatatct gctggggatt gtgcttggtt ggggcttgcc tggtatttgg gcagggtctc
     5161 tcttggataa tggttttcgc tggttatttc tacgctatcg ttaccagcgc tatatgagct
     5221 tgaaaggata ggaaatgcaa aaaacagctt ttatttggga tttagacggg actttattgg
     5281 actcttacga agcgatttta tcaggaattc agcagtttat atatactcat aaggggaaca
     5341 acgcttttac cattctcaag gacttggggg tggaatccta ttttacagag attttaacca
     5401 gtcagagtgg ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt
     5461 atcagctgga tcctgagaag acctattata taggggaccg gactttagat gtggaatttg
     5521 ccaagaatag cggtattcaa agcatcaact ttttagaatc ttcttttgaa ggcaatcaca
     5581 ggattcaagc actagcagat attcctcata tttttgagag taagtaacga gaagattgtg
     5641 tcagttttgt gacagaaacc taacaaacta tttcaagtaa tcgagtttgt tacaaggaat
     5701 agacagttct gttaaatagg cccgagaggg ctttttttct acattttttg tgttatcata
     5761 gacaggtact catttgaaag gaatttgaaa ggaatttgaa agaatgaaga aaagaatgtt
     5821 attagcgtca acagtggcct tgtcatttgc cccagtattg gcaactcaag cagaagaagt
     5881 tttttggact gcacgtagtg ttgagcaaat ccaaaacgat ttgactaaaa cagacaacaa
     5941 aacaagttat acagtacagt atggtgatac tttgagcacc attgcagaag ccttgggtgt
     6001 agatgtcaca gtgcttgcga atctgaacaa aatcactaat atggacttga ttttcccaga
     6061 aactgttttg acaacgactg tcaatgaagc agaagaagta acagaagttg aaatccaaac
     6121 acctcaagca gactctagtg aagaagtgac aactgcgaca gcagatttga ccactaatca
     6181 agtgaccgtt gatgatcaaa ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagc
     6241 tccaaaagaa gtagcatcaa gttcagaagt tacaaagaca gtgattgctt ctgaagaagt
     6301 ggcaccatct acgggcactt ctgtcccaga ggagcaaacg gccgaaacaa ctcgcccagt
     6361 tgaagaagca actcctcagg aaacgactcc agctgagaag caggaaacac aagcaagccc
     6421 tccagctgca tcagcagtgg aagcaactac aacaagttca gaagcaaaag aagtagcatc
     6481 atcaaatgga gctacagcag cagtttctac ttatcaacca gaagaaacga aaataatttc
     6541 aacaacttac gaggcaccag ctgcgcccga ttatgctgga cttgcagtag caaaatctga
     6601 aaatgcaggt cttcaaccac aaacagctgc ctttaaagaa gaaattgcta acttgtttgg
     6661 cattacatcc tttagtggtt atcgtccagg agacagtgga gatcacggaa aaggtttggc
     6721 tatcgatttt atggtaccag aacgttcaga attaggggat aagattgcgg aatatgctat
     6781 tcaaaatatg gccagccgtg gcattagtta catcatctgg aaacaacgtt tctatgctcc
     6841 attcgatagc aaatatgggc cagctaacac ttggaaccca atgccagacc gtggtagtgt
     6901 gacagaaaat cactatgatc acgttcacgt ttcaatgaat ggataaaccc gacttgataa
     6961 catcattttg acgaatgaga tctagctttc gtgatggaaa gcgattctcg ttcgtttttt
     7021 ctttgtcata ctcttcgaaa atctcttcaa accacgtcag ttttatctga aacctcaaag
     7081 ctgtgctttg agcaacctgc gactagcttt ctagtttact ctttgatttt cattgagtat
     7141 caatttgaat ggaaaatgga aagttattat cttgtaataa tccaagcaac attcttgcaa
     7201 tctattttac tttatatcac aattgattgg tcaactattg ataagtcaat ggataggagg
     7261 aagaaatgat agagattcaa gatttactgt atcaactccg cttgtctgag caagcgagta
     7321 cgcaattgtt tgaaaaaagg cttgggatta gtttgacacg gtatcagatt ttactgtttt
     7381 tgctggagca ttctccttgt aaccaaatgg cggttcagga gcgtttgaaa attgatcagg
     7441 ctgctttgac acggcatttc aagattttgg aaacggaagg tttggtggag cgtcatcgta
     7501 atcctgaaaa tcagcgggaa gtgttggtag aggctgcgaa gtatgccaag gagcagttag
     7561 tggtgaatcc ccctctgcaa catatcaagg ttaaggaaga gatggaaagt atcttaacag
     7621 agtttgagag aacagaactc agccgtttat taaataaatt ggttttgggt attgaaaata
     7681 tagaaattta aggagaaata gatgtcaatt attacaatta ttttaacaac gatcgttgct
     7741 ttggagcatt tttacatttt tacatttttt atttggaaag tattgccacg caatcagatg
     7801 cgactagtcg tgtatttaat atggaaaagg aagaattggc tcatccgtca gtaagttcat
     7861 tgttcaaaaa tcaaggaatt tataaggctc tgctaggagt ctttctcttg tatggcattt
     7921 atttctcaca taatttagaa attgtgacta tttttgtctt atttgtgatt ggtgctgcga
     7981 cttacggctc tttaacagcg gataaaaaaa ttattttgaa acaaggtgga tcagctattt
     8041 tggccttgat tagtatttta ctctttaaat acacttgaag gtcgattcta atctcgctaa
     8101 tcctttttaa tccagaataa gggaaatatg ttatacttgt ttttaagaaa aaagtctcat
     8161 tgaattggtt ttgaggagtt agaaatgaaa gtattagtga caggttttga gcccttttga
     8221 ggccattaaa ggtttaccag ctgaaatcca tggtgctgag gtccgttggc tagaggtgcc
     8281 gacagttttt cacaaatctg ctcaagtatt ggaagaagag atgaatcgtt atcaacctga
     8341 ctttgtcctt tgtattgggc aagctggtgg aagaactagt ttgacacctg aacgagtggc
     8401 cattaatcaa gacgatgcac gtacttctga taacgaagat aatcaaccga ttgaccgtcc
     8461 cattcgccca gatggtgctt cggcctactt tagtagtttg ccgattaaag cgatggttca
     8521 agctataaaa aaagagggct taccggcctc tgtttccaat acggcaggga cttttgtctg
     8581 cagccatttg atgtatcagg ctctctattt ggtagaaaag aaattttcat atgttaaggc
     8641 aggttttatg catattcctt atatgatgga acaggtggtg aacagaccga ctactccagc
     8701 tatgagttta gtggatattc ggcgagggat agaagcagca atcggcgcta tgatagaaca
     8761 tggagatcag gaactcaagt tggtaggcgg agaaactcat tgatagaaaa aagcttgagg
     8821 ggaaaaacct tcaagctttt ggacgttttc gagccaatac tgctcggtaa aacataattt
     8881 tagtgcattg gatataaggt aggagtgaaa aactagcaat gccaaaggta atccaattga
     8941 ggaagtacca aggaagaagc tgtaaatcta ggacaaagtg ctggaacttg tagcccttca
     9001 taaaggaacg gctagttttt aggattcgtc ttggtgggac ctgtcctagg tctagactat
     9061 aacagagaag aaattccacc tgtgaatagg cataatactg tggaatatag aggatatttc
     9121 ctacaatgat caagatgaga cttgcaagaa agtagagtcc aaagaccatg aggaaacgct
     9181 cggtttcaac tgatgagaga tctagatttg gaaactcagg atgtagggtg acgaattttt
     9241 tggctaaaaa gctactataa aagaggaggt aaatcccaag taaattaggg atactccata
     9301 aaaagagata gaaacgtttg agaagtaggg tcaaaaaggt ttgagaaaag cgctcctcat
     9361 caaagagagc taggctgttt tttacagatg gctccgtttt agaatctttc atgagtgtca
     9421 gtgttgcata gacggaactg gtcaaaagaa tagtcccgat aaaggagact agtagaggaa
     9481 agaggtaggc ttgaagtatt tggccaagta tgctgaaaaa tggctgttct aaaacagtcc
     9541 cgtggatccg agataaggga ttaagaaaac tagataagat gaccagcata ctgggaagga
     9601 tatagaggag aaagagacgg ggggtgtcag cctgaaaatg ttttgactcc tgacgaattg
     9661 tttttaaatc aatttttgga tagttcattc tcttattata ccatagttct tatacatagt
     9721 tcgtgacagt tcctactttt tttgataaaa tcatacagtg tgtccttggg cacactgtat
     9781 gaactgggac tgtctttccc agcttcggag gtaaaaaatg tcagattcac caatcaaata
     9841 tcgtttgatt aagaaagaaa aacacacagg agctcgtcta ggagaaatca tcactcccca
     9901 cggtaccttt ccgacaccta tgtttatgcc agttgggaca caagccactg tcaaaactca
     9961 gtcacctgaa gaattgaagg agatgggttc gggaattatc ctatcaaaca cctatcatct
    10021 ctggcttcgc cctggagatg aactcattgc acgcgctggt ggtctccaca agttcatgaa
    10081 ttgggaccag cctatcttga cagatagtgg tggttttcag gtttattctt tagcagatag
    10141 ccgtaatatc acagaagaag gagtaacctt taaaaatcat ctaaatggtt ctaagatgtt
    10201 cctatcccca gaaaaagcca tctctattca gaataatctg ggttcagaca tcatgatgtc
    10261 ctttgatgaa tgtcctcagt tttatcaacc ttatgactac gttaagaaat cgatcgagcg
    10321 taccagccgt tgggctgagc gtggtttgaa ggctcaccgt cgtccacatg accaaggttt
    10381 gtttggaatt gtgcaaggtg caggatttga agaccttcgc cgccaatcag ctcatgatct
    10441 tgtcagcatg gatttctcag gctactctat cggtggtttg gcagtgggag aaacccatga
    10501 agagatgaat gcggtcttgg actttacaac tcaactgcta cctgaaaata aacctcgtta
    10561 tctgatgggt gtgggagcgc cagatagctt gatcgatggg gtcattcgtg gggtggatat
    10621 gtttgactgt gtcttaccga ctcgaattgc tcgtaacggg acttgtatga ccagtcaagg
    10681 acgtttggtt gtgaaaaatg cccagtttgc tgaggacttt acgccactgg atcctgagtg
    10741 tgattgttac acatgtaata actatacacg cgcttacctt cgtcacctgc tcaaggctga
    10801 tgaaaccttt ggtatccgct tgactagcta ccacaatctt tacttcttgc ttaacttgat
    10861 gaagcaagtg cgacaagcca tcatggatga caatctcttg gaattccgtg agtattttgt
    10921 ggaaaaatat ggctataata agtcaggacg taatttctaa aatggaattg atataagcta
    10981 aaaatcctaa gttttctctt aggatttttc ttcttttttt gatagaataa agtgtacaat
    11041 gaaaggaaga ataaactcgt atgcgcatta aatggttttc cttgattagg attataggtt
    11101 tacttttggt actcttgtat cacttctttc agacgatctt tcctggagga tttttcgggg
    11161 tagatgtctt tttcacattt tcaggtttcc tgattacagc tctactcatt gaagaatttt
    11221 ctaagaacaa tgagattgat ttgataggat tttttagaag acgcttttat cggattgtgc
    11281 cacctgtggt tttgatggtc ttggtgacca tgccttttac tttcttggtt cgccaagact
    11341 atgttgctgg aattggtggc cagattgcgg gcgtcttagg ctttatgacc aacttctatg
    11401 aactcctaac aggtgggagt tatgaatctc agttcattcc tcatttgttt gttcataatt
    11461 ggagtttggc agttgaggtt cactactata ttctttgggg attggcagtt tggttcttat
    11521 ccaaacaagc taaatcaaat ggtcagttga aggggatggt ctttctctta tctgctgttg
    11581 ccttcttgat cagtttcttc tccatgttta ttggtagttt tctagtgacc tcttattcct
    11641 ctgtttattt ctccagttta actcatgttt atccattctt tttgggaagt atgttagcaa
    11701 ctattgtagg tgttcgtcag acgacttccc tcgtcaagca gttggataaa atctgggatt
    11761 tacgaaagac tttggtagtt tttggaggag gctttggttt cctagttctt ttgactttct
    11821 ttgtcaaatt cacttatctt tttgcctatc ttatcggctt cttgcttgcc agtcttgcag
    11881 ctcttgccat gattctggcg gcgcgtgtct tacatgaaaa gacacatcat atacaggagc
    11941 cgaagattat cagcttttta gcggatacta gctatgcggt ttatcttttc cattggcctt
    12001 tctatatcat tttctcacag ttgacatcaa atcttcttgc tgtattactg actctgattt
    12061 gttcttatgg ctttgccagt ctgtcatttt atgtattgga accttggatt gcaggcaaga
    12121 acacacctat tttacaaact cttcgtcccc tgccttatat tcacgcaatt cttgcagcag
    12181 gtacaggaat cttgaccttc attgtctgca tggtgacctt gttggcacca caagtgggag
    12241 cgtttgagac agacttgact gtcaatggct tgaagcaagc tgcaacaaat attggccaga
    12301 ccaaggtgat ggcagaacgg gcagatgcaa acagtttggg aattgctgat ggcactatgt
    12361 taattggtga ctcagtggct ttaagggcaa atacagcact acagacagct cttcctggag
    12421 cacagattaa cgcgcaggtc agcgtaacaa ccaagaccgc aaatgaaatc atgctaaata
    12481 atagccagaa taaattttta cctaagacgg tggtcattgc gactggggta aataatcctg
    12541 agaattacaa ggatgactgg gacagtatcg tgaaaaatct tcctaaggga caccatatga
    12601 ttttggtgac tccttatgag ggagataaga caaaagagac ctatgccatc gttgagaagg
    12661 ctgctgccta tatgagagaa ttggcagaga agacacctta cattacgata gcagattgga
    12721 atcaagttgc gaaagagcat ccagaaattt gggctggaac agaccaggtt catttcggga
    12781 gtgagagtag cactatcgaa gcaggagcaa aattgtatgc agatacgatt gccacagctt
    12841 tgcagacagc tcaagacaag ccggttaaat caaaataact tgtattagaa aagagaagag
    12901 ttggagaaat ccagctcttt taaaatatct ccaaaaaata ggtctatacc atttacaaat
    12961 aaaaaagaaa ggtttataat gtaattgaca taataaattg tagaatcaat cttttaagga
    13021 ggttaacatt atgcctaact atattaaagc ggatcagttt ttctacccac acggagttcg
    13081 tcgaggtggt tacttggaac ttgtggacgg caagtttgga aaacatgtag agcagattcc
    13141 tgaaggggct gaggtgattg actatacagg ttatagcatt gccccaggtc ttgtggatac
    13201 tcatattcat ggatatgcag gtgtagatgt gatggacaac aacattgaag gtacattgca
    13261 tactatgagt gaaggacttc ttagtaccgg tgttaccagt ttcttaccca caactttaac
    13321 agccacttat gagcaattgc ttgcagtcac tgaaaatctt ggaaaccatt ataaagaagc
    13381 aacaggtgct aagattcgtg ggatttatta tgaaggtcca tatttcacag aaacttttaa
    13441 gggggcacaa aatccaactt atatgagaga cccgggtgtt gaggagtttc attcttggca
    13501 aaaagcggca aatggcttgc ttaataaaat tgcccttgca ccagaacgtg atggggtgga
    13561 agactttgtt cgtacagtta cgggcgaagg tgtgacggtt gctcttggac attcaaacgc
    13621 gacttttgat gaagccaaaa aagcagtcga tgctggagcg agtgtttggg tgcatgccta
    13681 caatggaatg cgtgggttga ctcaccgtga attgggtatg gttggagcca tgtaccaatt
    13741 gccacatacc tatgcagagt tgatctgtga tggtcaccac gtagatccaa aggcttgcga
    13801 aattcttatc aaacaaaaag gaacagaaaa catcgctctt atcacagact gtatgacagc
    13861 tgggggattg gaagacggag attatatgtt gggagaattc ccagttgtcg ttgcaaatgg
    13921 aactgcacgc ctcaaatcga caggtaactt ggcaggttct atcctcaaac tcaaagatgg
    13981 tttgaagaat gtggtcgaat ggggaattgc gaatccgcat gaagcagtca tgatggccag
    14041 cttcaaccca gctaaatccg ttcacatcga tgatgtctgt ggccaaatcc gtgaaggcta
    14101 cgacgctgac ttcatcgtat tagataaaga tttggaattg gtagcaacct acctagatgg
    14161 cgtgaaacgt tatcaagcat aagagagaag gcagaagtta gaaactagct tctgcttttt
    14221 tatacagggt actttactgg taaataaaaa gttaaaaaaa tcacaaaaaa agcttgaaga
    14281 aacaaagaaa aaggcttaca attgaactat aaatattaca aataaaaaaa cggaggagtg
    14341 ctttatgaaa gcctatactt atgttaaacc aggacttgct tcttttgttg atgtagacaa
    14401 accagttatt cgcaagccaa cagacgctat tgtgcgtatt gtaaaaacca ctatttgtgg
    14461 aacagacctc catattatca aaggggatgt tcctacttgc caaagtggta ccattcttgg
    14521 ccacgaaggg attgggattg ttgaagaagt tggggaagga gtttccaact tcaaaaaagg
    14581 tgacaaggtc ttgatttctt gcgtctgtgc ctgtggtaaa tgctactact gtaaaaaagg
    14641 aatttatgcc cactgtgaag acgaaggggg ctggattttc ggtcacttga ttgatggtat
    14701 gcaggctgaa tatctacgtg tccctcatgc agataatact ctttaccata ctccagaaga
    14761 cttgtcagat gaagctttgg ttatgctgtc agacattctg cctactggat atgaaattgg
    14821 tgtcttaaaa gggaaagtag aacctggttg cagcgtagcc attattggtt caggtccagt
    14881 tggattggct gctcttttaa cagcccaatt ctattcacca gctaaattga ttatggtaga
    14941 cctagacgat aaccgcttgg aaactgccct atcattcggt gcgactcata aggttaattc
    15001 ttcagaccct gaaaaagcca ttaaagaaat ttatgatttg acagatggtc gtggtgtgga
    15061 tgtcgctatc gaagctgttg gtattcctgc aacatttgat ttctgtcaaa agattatcgg
    15121 tgtagacgga acggttgcca actgtggtgt gcatggtaaa ccagttgaat tcgatttaga
    15181 taaactttgg attcgcaaca tcaatgtaac aactggtttg gtatctacaa atacgactcc
    15241 acaattgttg aaagcacttg aaagtcataa gattgaacca gaaaaattgg taactcacta
    15301 tttcaaactc agtgaaattg aaaaagccta cgaagtcttc agtaaggcag cagaccacca
    15361 tgccattaag gtcattatcg aaaacgatat ctcagaagcc taagtagtaa aaatattttt
    15421 gtacataagt aaatagaaat tcagtcatcc atcagatggc tggatttttt atcaaaaaat
    15481 taagaaatga gcatatttct ttccttgtct ggcggaattg gttataatat acggtacaaa
    15541 ggaatgaatg aatatgtatc gtgttataga aatgtacgga gattttgaac cgtggtggtt
    15601 cttagaaggt tgggaagaag atattgtagc aagtagaaaa tttgaccagt attatgatgc
    15661 tctcaaatac tacaaaactt gctggtttag attggaacaa gaatcccctc tttataaaag
    15721 tagaagcgac ttgatgacca ttttttggga cccggaagac caacgctggt gtgatgaatg
    15781 tgatgagtat ttacaacaat accattcttt ggctcttttg caggatgagc aggttatccc
    15841 agacgaaaaa ctacgctcag gctatgaaaa acaaaccagt caggaaagga atcgttcttg
    15901 ccgtatgaaa ttaaaataga gaaaagtaac ttttttggag ttgctttttt tatttttcta
    15961 acgctttgcg aatagtatag gtgaggaggt aagtatggtt caagaaattg cacaagaaat
    16021 cattcgttcg gctcggaaaa aaggggcgca agacatttat tttgtcccta agttagatgc
    16081 ctatgagctt catatgaggg taggagacga gcgctgtaaa attggtagct atgattttga
    16141 aaagtttgca gccgttatca gtcactttaa gtttgtggcg ggtatgaatg tgggagaaaa
    16201 aagacgtagt caactgggtt cctgtgatta tgcctatgac cagaagatag cgtctctacg
    16261 tttatctact gtaggcgatt atcgggggca tgagagtttg gttatccgtt tgttgcacga
    16321 tgaggagcag gacttgcatt tttggtttca ggatattgaa gaattaggca agcagtacag
    16381 gcaacgggga ctctatcttt ttgctggtcc ggttgggagt ggtaagacga ccttgatgca
    16441 tgaattgtcc aagtcactct ttaaaggaca gcaagttatg tccatcgaag atcctgtcga
    16501 aatcaagcag gacgacatgc ttcagttgca gttgaacgaa gcaatcggcc taacctatga
    16561 aaatctaatc aaactttcct tgcgtcatcg accagatctc ttgattatcg gagaaattcg
    16621 tgacagcgag acggcgcgtg cagtggtcag agctagtttg acaggtgcga cagtcttttc
    16681 aaccattcac gccaaaagta tccgaggtgt ttatgagcgt ctgctggagt tgggtgtgag
    16741 tgaagaagaa ttggcagttg ttctgcaagg agtctgctac cagagattaa tcgggggagg
    16801 aggaatcgtt gactttgcaa gcagagatta tcaagaacac caagcagcca agtggaatga
    16861 gcaaattgac cagcttctta aagatggaca tatcacaagt cttcaggctg agacggaaaa
    16921 aattagctac agctaagcaa aaaaatatca tcaccctatt taacaatctc ttttctagcg
    16981 gttttcatct ggtggagacc atctcctttt tagataggag tgctttgttg gacaagcagt
    17041 gtgtgaccca gatgcgtgtg ggcttgtctc aggggaaatc attctcagaa atgatggaaa
    17101 gtttgggatg ttcaagtgct attgtcactc agttatccct agctgaagtc catggcaatc
    17161 tccacctgag tttgggaaag atagaagagt atctagacaa tctggccaag gtcaagaaaa
    17221 aattaattga agtagcgacc tatcctttga ttttgctgtt ttttcttctc ttaattatgc
    17281 tggggctacg gaattacctg ctcccacaac tggatagtag caatattgcc acccgaatta
    17341 tcggtaatct gccacaaatt tttctaggca tggtagggct tgtttccgtg cttgcccttt
    17401 tagcactaac tttttataaa agaagttcta agatgagtgt cttttctatc ttagcacgcc
    17461 ttccctttat tggaatcttt gtgcggacct atttgacagc ctattatgca cgtgaatggg
    17521 ggaatatgat ttcacaggga atggagctga cgcagatttt tcaaatgatg caggaacaag
    17581 gttcccagct ctttaaagaa atcggtcaag atctggctca aaccctgaaa aatggccgtg
    17641 aattttctca gacgatagga acctatccgt tctttaggaa ggaattgagt ctcatcatag
    17701 agtatgggga agttaagtcc aagctgggta gtgagttgga aatctatgct gaaaaaactt
    17761 gggaagcctt ttttacccga gtcaaccgca ccatgaattt ggtgcagcca ctggttttta
    17821 tctttgtggc actgattatc gttttacttt atgcggcaat gctcatgccc atgtatcaaa
    17881 atatggaggt aaatttttaa aatgaaaaaa atgatgacat tcttgaaaaa agctaaggtt
    17941 aaagctttta cattggtgga aatgttggtg gtcttgctga ttatcagcgt gcttttcttg
    18001 ctctttgtac ctaatctgac caagcaaaaa gaagcagtca atgacaaagg aaaagcagct
    18061 gttgttaagg tggtggaaag ccaggcagaa ctttatagct tggaaaagaa tgaagatgct
    18121 agcctaagca agttaaaaga agatggacgc atcacggaag aacaggctaa agcttataaa
    18181 gaatacaatg ataaaaatgt aggagcaaat cgtaaagtct atgattaagg cctttaccat
    18241 gctggaaagt ctcttagttt tgggacttgt gagtatcctt gccttgggct tgtccggctc
    18301 tgtccagtcc acttttgcag cggtagagga acagattttc tttatggagt ttgaagaact
    18361 ctatcgggaa acccaaaaac gcagtgtagc tagtcaacaa aagactagtc tgaacttgga
    18421 tgggcagatg attagcaatg gcggtcaaaa gttgacagtt cctaaaggaa ttcaggcacc
    18481 atcaggccaa agtattacat ttgaccgagc tgggggcaat tcgtccctgg ctaaggttga
    18541 atttcagacc agtaaaggag cgattcgcta tcaattatat ctaggaaatg gaaaaattaa
    18601 acgcattaag gaaacaaaaa attagggcag tgattttact ggaagcagta gtcgctctag
    18661 ctatctttgc cagcattgcg accctccttt tgggacaaat tcaaaaaaat aggcaagagg
    18721 aagcaaaaat cttgcaaaag gaagaagtct tgagggtagc taagatggcc ttgcagacgg
    18781 ggcaaaatca ggtaagcatc aacggagttg agattcaggt attttctagt gaaaagggat
    18841 tggaggtcta ccatggttca gaacagttgt tggcaatcaa agagccataa ggtcaaggct
    18901 tttaccttgt tagaatccct gcttgccctc attgtcatca gtgggggatt actccttttt
    18961 caagctatga gtcagctcct catttcagaa gttcgctacc agcaacaaag cgagcaaaag
    19021 gagtggctct tgtttgtgga ccaacttgag gcagaattag accgttcgca gttcgaaaaa
    19081 gtagaaagca atcgcctata catgaagcaa gatggcaagg acatcgccat cggtaagtca
    19141 aagtcagacg attttcgtaa aaccgatgct agtggacggg ggtatcagcc tatggtttat
    19201 ggcctcaaat cagctcagat tacagaggac aatcaactgg ttcattttcg tttccagttt
    19261 caaaaaggct tagaaaggga gttcatctat cgtgtggaaa aagaagaaag ttaaggcagg
    19321 tgttctcctc tacgcagtca ccatagcagc catctttagt cttttgttgc aattttattt
    19381 gaaccgacaa atcgcccact atcaagacta tgctttaaat aaagaaaaat tggttgcttt
    19441 tgccatggct aaacgaacca aggataaagc cgagcaagaa agtggggaac aggtctttaa
    19501 tctaggtcag gtaagctatc aaaacaagaa aacaagcttg gtgacgactg ttcgtacgtc
    19561 taagagccaa tatgagtttc tgtttccttc agtcaaaatc aaagaagaga aaagagataa
    19621 aaaggaagag gtagcgaccg attcaagcga aaaagtggag aagaaaaaat cagaagagaa
    19681 gcctgaaaag aaagagaatt cctagtcaat tcaactataa tgcgttgaat ccagaatagt
    19741 ccactgtagt ttctagaaaa ttgctggaaa tggatgttaa gctccaattc atttgtttat
    19801 atcttatttc agtttactat actttgtgct aaactaaaga tatgaaacat gattttaacc
    19861 acaaagcaga aactttcgac tcccctaaaa atatcttcct cgcaaacttg gtatgtcaag
    19921 cagtcgagaa acagattgat attttatcag acaaagtaat tttagatttc ggtggtggca
    19981 cgggtctatt agccttgccc ctagccaagc aggctaagtc agttactctt gtagacattt
    20041 ctgagaaaat gttggagtaa gctcgtttga aagcggagca gcaagcaatc aagaatatcc
    20101 agtttttgga gcaagattta ccgaaaaatc ccttggagaa agagtttgat tgccttgctg
    20161 ttagtcgggt tcttcatcat atgcctgatt tggatgcgga tctctcactg tttcatcaac
    20221 atttgaagga agatgggaaa ctcatcattg ctgattttac caagacagaa gctaatcatc
    20281 atggatttga tttagctgaa ctggaaaaca agctaattga gcatggtttt tcatctgtgc
    20341 atagtcagat tctctatagc gctgaagacc tgtttcaagg gaatcactca gaattctttt
    20401 taacagtatc ccaaaaatca ctcgcctagt cagggagtga tttttctata aggatggaaa
    20461 aaagaaggga aatttgataa gataggaata tggattttga aaaaattgaa caagcttata
    20521 tctatttact agagaatgtc caagtcatcc aaagtgattt ggcgaccaac ttttatgacg
    20581 ccttggtgga gcaaaatagc atctatctgg atggtgaaac tgagctaaac caggtcaaag
    20641 acaacaatca ggcccttaag cgtttagcac tacgcaaaga agaatggctc aagacctacc
    20701 agtttctctt gatgaaggct gggcaaacag aacccttgca ggccaatcac cagtttacgc
    20761 cagatgctat tgccttactt ttggtattta ttgtggaaga gttgtttaca gaggaggaaa
    20821 ttactatcct cgaaatgggt tctgggatgg gaattctagg cgctactttc ttgacctcgc
    20881 ttgataaaaa ggtggattac ttgggaatgg aagtggatga tttgctgatt gatctggcag
    20941 ctagcatggc agatgtaatt ggtttgcagg ctggctttgt ccaaggagat gccgttcgcc
    21001 cacaaatgct caaagaaagc gatgtggtca tcagtgactt gcctgttggc tattatcctg
    21061 atgatgccgt tgcgtcgcgc catcaagttg cttctagcca agaacatact tacgctcatc
    21121 acttgctcat ggaacaaggg tttaagtacc ttaagtcaga tggatatgct atttttctag
    21181 ctccgagtga tttgttgacc agtcctcaaa gtgatttgtt gaaagtctgg ttgaaagagg
    21241 aggcaagtct ggttgctatg attagtttac ctgaaaatct ctttgctaat gctaaacaat
    21301 ctaagactat ttttatccta cagaagaaaa gtgaaatagc agtggagcct tttgtttatc
    21361 cacttgctag cttgcaagat gcaagtgttt taatgaaatt taaagaaaat tttcaaaaat
    21421 ggactcaagg tactgaaata taaaatagat tttgttataa tagttgaaaa cgcttaaaaa
    21481 ggggtatcat gttatgacaa aaacaattgc aatcaatgca ggaagttcaa gtttgaaatg
    21541 gcaattatac ttaatgccag aagaaaaagt attggcgaaa ggtttgattg aacgtatcgg
    21601 tttgaaagat tcaatttcaa ctgtaaaatt tgacggccgt tctgaacaac aaattttgga
    21661 tattgaaaat catatacaag ccgttaaaat tttattggat gacttgattc gtttcgatat
    21721 tatcaaggct tatgacgaga ttacaggtgt tggacatcgt gttgttgctg gtggagaata
    21781 tttcaaagaa tcaacagttg ttgagggaga tgttttagaa aaagttgaag agttgagttt
    21841 gttggctcct ctacacaacc cggccaatgc agcaggtgtt cgtgccttca aggaattgtt
    21901 gccagacatt accagtgtag ttgtttttga tacttccttc cacacaagta taccagagaa
    21961 agcttatcgc taccctctac caacaaaata ttacacagaa aacaaggttc gtaaatacgg
    22021 tgctcatggt acaagtcacc agtttgtagc aggagaagct gcaaaactct tgggacgtcc
    22081 attagaagac ttgaagttaa ttacctgtca tattggtaac ggaggctcaa ttacagctgt
    22141 gaaagccggc aaatctgtag acacttctat ggggttcact cctcttggtg gtattatgat
    22201 gggaacgcgt acaggggata ttgatccagc tatcattcct tatttaatgc aatatacaga
    22261 ggattttaac acaccagaag atatcagtcg tgttcttaac cgtgaatcag gtcttttggg
    22321 agtttctgct aattctagcg atatgcgcga tatagaagca gctgtagcag aagggaatca
    22381 cgaggctagc ttggcttatg aaatgtatgt tgaccgtatc caaaaacata tcggtcagta
    22441 ccttgcagtg ctaaatggag cagatgccat tgttttcaca gcaggtgtcg gtgaaaatgc
    22501 agagagtttc cgtcgtgatg taatctcagg gatttcgtgg tttggttgtg atgttgatga
    22561 tgaaaagaat gtctttggcg ttacaggaga catctcaaca gaggcagcta aaatccgtgt
    22621 cttggttatt ccaacagatg aagaattagt cattgcccgt gacgttgaac gcttgaaaaa
    22681 ataagtgaaa ctaaaaaaat attcaataca aggagttggg aaagttattt ttccagcttc
    22741 tttttctgat gaaattgtcc aaaaccttgc tatgattggc ttttttgaaa aatatggtat
    22801 aatagtagta atttaataga tggagttgag ttttgaagaa aaactttcgt gtaaaaagag
    22861 agaaagattt taaggcgatt ttcaaggagg ggacaagttt tgctaatcgc aaatttgtgg
    22921 tctaccaatt agaaaaccag aaaaaccatt ttcgagtagg tctatcagtt agcaaaaaac
    22981 tggggaatgc cgtcactaga aatcaaatta agcgacggat tcggcatatt atccagaatg
    23041 caaaagggag tctggtagaa gatgtcgact ttgttgtcat tgctcgaaaa ggagtcgaaa
    23101 ccttgggata cgcagagatg gagaaaaatc tactccacgt attaaaatta tcaaagattt
    23161 accaggaagg aaatgggagt gaaaaagaaa ctaaagttga ctagtttgct aggactgtct
    23221 ctgttaatca tgacagcctg tgcaactaat gaggtaacta gcgatattac agccgattcg
    23281 gctgattttt ggagtaaatt ggtttacttc tttgcggaaa tcattcgctt cttatcgttt
    23341 gatattagta tcggagtggg gattattctc tttacggtct tgattcgtac agtcctcttg
    23401 ccagtctttc aggtgcaaat ggtggcttct aggaaaatgc aggaagctca gccacgcatt
    23461 aaggcgcttc gagaacaata tccaggtcga gatatggaaa gcagaaccaa actagagcag
    23521 gaaatgcgta aagtatttaa agaaatgggt gtcagacagt cagactctct ttggccgatt
    23581 ttgattcaga tgccggttat tttggccctg ttccaagccc tatcaagagt tgacttttta
    23641 aagacaggtc atttcttatg gattaacctt ggtagtgtgg atacaaccct tgttcttccg
    23701 attttagcag cagtattcac ctttttaagt acttggttgt ccaacaaagc tttgtctgag
    23761 cgaaatggcg ctacgactgc gatgatgtat gggattccag tcttgatttt tatctttgca
    23821 gtttatgcgc caggtggagt cgccctatac tggacagtgt ctaatgctta tcaagtcttg
    23881 caaacctatt tcttgaataa tccattcaag attatcgcag agcgcgaggc cgtagtacag
    23941 gcacaaaaag atttggaaaa tagaaaaaga aaagccaaga aaaaggctca gaaaacgaaa
    24001 taaataagga ggaatctggt aatggtagta tttacaggtt caactgttga agaagcaatc
    24061 cagaaaggat tgaaagaatt agatattcca agaatgaagg ctcatatcaa agtcatttct
    24121 agggagaaaa aaggctttct tggtctattt ggtaaaaaac cagcccaagt ggatattgaa
    24181 gcgattagtg aaacgactgt tgtcaaagca aatcaacagg tagtaaaagg cgttccgaaa
    24241 aaaatcaatg atttgaacga gcctgtgaag acggttagtg aagaaaccgt tgaccttggt
    24301 catgtggtta atgctattaa aaaaatagag gaagaaggtc aaggtatttc tgatgaagtc
    24361 aaggctgaaa tcttaaaaca tgaaagacat gccagcacta tcttagaaga aactggtcac
    24421 attgagattt taaatgaact tcaaatcgag gaagcgatga gggaagaagc aggcgctgat
    24481 gaccttgaaa ctgagcaaga tcaaactgaa aatcaagact tgaaagagat gggcttgaag
    24541 gtcgagcaaa gttatgatat tgcccaggtg gctacggatg tgactgccta tgttcaagcg
    24601 attgtggatg acatggatgt tgaagctaca ctttcaaatg attataaccg tcgtagcatc
    24661 aatctacaaa ttgacaccaa cgaaccaggt cgtattatcg gctaccatgg taaagtcttg
    24721 aaggccttgc aactgttggc tcaaaattat ctttacaacc gctattccaa aaccttctac
    24781 gttacaatca atgtcaatga ttatgtcgaa caccgtgcag aagtcttgca gacctatgcg
    24841 caaaaattgg cgaatcgtgt tttggaagaa ggtcgcagtc ataaaacaga tccaatgtca
    24901 aatagcgaac gcaagattat ccatcgtatt atttcacgta tggatggcgt gactagttac
    24961 tctgaaggtg atgagccaaa tcgctatgtt gttgtagata cagaataagt aaaatcaggt
    25021 ttatcctgat tttttgctag ttagaggagg ttaaactgat gttgaataag ataagagact
    25081 atttagactt tgctggtttg cagtaccgta atcctgataa agcgggagca gagcgagaga
    25141 agatgctggc attccgccac aaaggacaag aggcccgaaa ggtttttaca gaactggcca
    25201 aagcctttca agcaagccat ccagaatggc aactccaaca gactagtcag tggatgaatc
    25261 aggcccagcg tttgagacca catttttggg tttatctaca gagagacgga caagtgacag
    25321 aacctatgat ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag
    25381 tcagtttcat cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag
    25441 acattccaac cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt
    25501 gggaggcgaa tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc
    25561 gaaaagtttt agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg
    25621 tagaaggctt attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat
    25681 aagataattt gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa
    25741 ttcttggtct ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa
    25801 aggagaacaa ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg
    25861 cagaatcccg catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa
    25921 cctgcccatg acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac
    25981 gtgggtgctg gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa
    26041 ttccaaattg gtgcagcggt tatcgaccct tactttggac ttgctgcagc aaacaacaaa
    26101 attgtagcag agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt
    26161 ggaataaata tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt
    26221 actggtcaca tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta
    26281 ccacaattgc gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg
    26341 gcagttagtt caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt
    26401 gcgattggtc accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt
    26461 ggtaagaaag aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac
    26521 gatacagttg ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc
    26581 ttgggtccag acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct
    26641 gctaaacaag atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg
    26701 ttcgttttga tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt
    26761 atttcaaaca aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga
    26821 tttggttctc caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt
    26881 acaattgttt tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca
    26941 gtgttctttg acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg
    27001 gctgttatcc tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg
    27061 gcccttctcg atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca
    27121 tggcttggat ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt
    27181 ctcttcttgc ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat
    27241 tacaacggtg aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa
    27301 agtattagca gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga
    27361 aaatgctctc cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga
    27421 agctaaaggt ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga
    27481 attggaaggg cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga
    27541 aatcaccgaa aaactcagtc aagcactaca gtaaaaggtt ggagggggca gatatgaatt
    27601 taaaacaagc tttaattgac aatgactcga tccgactagg tttagaggct aacgattgga
    27661 aagaagcagt caaggtagca gtagatccct tgattgaaag tggggcaatt ttgccagagt
    27721 attacgatgc tatcattgaa tcgactgaag agtatgggcc ttactatatc ttgatgccag
    27781 gtatggctat gccccacgct agacctgaag ctggtgtgca aagtgatgcc ttttcattga
    27841 ttaccttaca aaatcctgtt gtattttcag atgggaaaga ggtatctgtt ttgttggcac
    27901 tagcagcaac aagctcaaaa attcacacaa gtgtagccat tccacaaatt attgccctgt
    27961 ttgaattaga agattctatt gcacgtttac aggcttgcca gactaaagaa gatgtcttgg
    28021 ctatgattga agaatctaag gatagccctt atctcgaagg attggatttg gaaagttaga
    28081 aagaggaata aagaaatgac aaaaagaata cctaatttac aagttgcatt agaccattca
    28141 gacttgcaag gagcgattaa agcagctgtt tctgttggtc aggaagtaga tattatcgaa
    28201 gctggaactg tttgcttgct tcaagttgga agtgaactgg ctgaagtctt gcgtagcctt
    28261 ttcccagata agattattgt ggcagacaca aaatgtgctg atgctggtgg aacagttgct
    28321 aaaaataatg cggttcgtgg agcagactgg atgacttgta tctgttgtgc aaccatccct
    28381 actatggaag cagctctaaa ggctatcaag actgaacgag gagaacgagg cgaaatccag
    28441 atcgagcttt atggcgattg gacttttgaa caagctcagc tttggctaga tgcaggtatc
    28501 tcacaagcta tttatcacca atctcgtgat gctcttcttg ctggtgaaac ttggggtgaa
    28561 aaagacctta ataaggttaa aaaactcatt gacatgggct tccgtgtatc tgtaacaggt
    28621 ggtctagatg tagatactct caaactcttt gaaggtgttg atgtctttac ctttatcgca
    28681 ggtcgtggaa ttacagaggc tgcggatcca gcaggagcag cgcgtgcctt caaggatgaa
    28741 atcaaacgaa tttgggggta aatcatggta cgtccaattg gaatttatga aaaggcaacc
    28801 ccaacacact ttacttggct agaacgttta aattttgcca aggagttagg ctttgatttt
    28861 gtcgagatgt ctattgacga acgtgacgag cgtttagcaa gacttgactg gagtaaggaa
    28921 gaacgcttgg aagttgtcaa agcaatccat gaaactggtg ttcgtattcc ttctatctgt
    28981 ttttcaggcc atcgtcgcta cccattgggt tcaaaagatc cagttctaga ggaaaaatct
    29041 ctagaactca tgaaaaaatg tatcgaatta gctcaagact tgggagttcg tacgattcaa
    29101 ttagctggtt acgatgttta ctatgaggaa aagtcacccc agacacgcca acgttttatc
    29161 aaaaatttga gaaaagcctg tgactgggct gaagaagctc aggtggtact tgctattgaa
    29221 attatggatg atcctttcat caatagcatc gaaaaatatt tggctataga aaaagagatt
    29281 gactctccct tcctctttgt atatccagat attggtaatg tgtctgcatg gcataatgat
    29341 atctatagtg agttttatct tggtcatcat gccatcgcag ctctccatct caaggatact
    29401 tatgcagtga cagaaagttc aaagggccag ttccgagatg tacctttcgg gcaaggttgt
    29461 gtcaaatggg aagaagcttt cgatatttta aaggaaacca attataatgg acctttccta
    29521 atcgaaatgt ggtctgaaaa ttgtgaaaca gtagaagaaa cacgcgcagc cgttcaagag
    29581 gcgcaagctt ttctctatcc actcattaag aaagcaggtt tgatgtaaga tgaatcaagt
    29641 aatcaatgct atgcgtaaac gagtctgtga tgccaatcaa tcattgccaa aacatggact
    29701 tgtcaaattt acctggggga atgtatctga agttaatcgc gaactcggtg tcattgttat
    29761 caaaccatca ggcgtggatt atgacgaatt gacacctgaa aacatggtag tgactgatct
    29821 agatggtaag atcctagaag gggatttaag accatcttcc gacctcccaa ctcatgtgca
    29881 attatataag gcttggtcag aaattggtag tgtggttcac acccattcga cagaagctgt
    29941 tggttgggct caggcaggtc gtgatattcc tttctacgga acaacccatg cagattattt
    30001 ctacggttca atcccttgcg cccgtagttt gaccaaggac gaagtagaag tggcctatga
    30061 aaaagatact ggcctggtta tcgtagaaga gtttgaacat cgcggactta acccggttga
    30121 agtaccagga attgttgtac gcaatcacgg tccattcacc tggggcaaaa atccagagaa
    30181 tgctgtttat cactctgtcg tactagagga agtatcaaag atgaatcgct ttacagaaca
    30241 aatcaatcca agagttggac ctgctcccca gtacatacta gaaaaacact accaacgtaa
    30301 acatggacca aatgcttatt atggtcaaaa gtaagaacga tgaaggagga gaaaaagata
    30361 aatttagctc ctctttttac atttgatttt tattgagagt aaagttggaa ttgaagtaat
    30421 tttaaaagat tttttag
//
LOCUS       DAVHIT010000020        29335 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVHIT010000020 DAVHIT010000000
VERSION     DAVHIT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.020"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            8..2038
                     /locus_tag="VVN16_000741"
     CDS             8..2038
                     /locus_tag="VVN16_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU5572170.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            2042..2326
                     /locus_tag="VVN16_000742"
     CDS             2042..2326
                     /locus_tag="VVN16_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5572171.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            2339..3685
                     /locus_tag="VVN16_000743"
     CDS             2339..3685
                     /locus_tag="VVN16_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU5572172.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            3689..4546
                     /locus_tag="VVN16_000744"
     CDS             3689..4546
                     /locus_tag="VVN16_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5572173.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            4543..5475
                     /locus_tag="VVN16_000745"
     CDS             4543..5475
                     /locus_tag="VVN16_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU5572174.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSSSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            5708..7411
                     /gene="ilvD"
                     /locus_tag="VVN16_000746"
     CDS             5708..7411
                     /gene="ilvD"
                     /locus_tag="VVN16_000746"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5572175.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(7527..7607)
                     /locus_tag="VVN16_000747"
                     /pseudo
     CDS             complement(7527..7607)
                     /locus_tag="VVN16_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7665..8006)
                     /locus_tag="VVN16_000748"
     CDS             complement(7665..8006)
                     /locus_tag="VVN16_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5572176.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            8130..8981
                     /gene="rgg"
                     /locus_tag="VVN16_000749"
     CDS             8130..8981
                     /gene="rgg"
                     /locus_tag="VVN16_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU5572177.1"
                     /translation="MEQIGKVFRQLRESRNISLRQVTGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            9172..10419
                     /locus_tag="VVN16_000750"
     CDS             9172..10419
                     /locus_tag="VVN16_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU5572178.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            10497..11786
                     /locus_tag="VVN16_000751"
     CDS             10497..11786
                     /locus_tag="VVN16_000751"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5572179.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLKPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            11777..11857
                     /locus_tag="VVN16_000752"
                     /pseudo
     CDS             11777..11857
                     /locus_tag="VVN16_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            12079..12393
                     /locus_tag="VVN16_000753"
     CDS             12079..12393
                     /locus_tag="VVN16_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572180.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            12403..12609
                     /locus_tag="VVN16_000754"
     CDS             12403..12609
                     /locus_tag="VVN16_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5572181.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            12609..12806
                     /locus_tag="VVN16_000755"
     CDS             12609..12806
                     /locus_tag="VVN16_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572182.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            12809..13543
                     /locus_tag="VVN16_000756"
     CDS             12809..13543
                     /locus_tag="VVN16_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU5572183.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            13555..14196
                     /locus_tag="VVN16_000757"
     CDS             13555..14196
                     /locus_tag="VVN16_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5572184.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLVLGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            14214..14444
                     /locus_tag="VVN16_000758"
     CDS             14214..14444
                     /locus_tag="VVN16_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572185.1"
                     /translation="MKRVILVAVIQAVVLFFIIGGLAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            14480..14560
                     /locus_tag="VVN16_000759"
                     /pseudo
     CDS             14480..14560
                     /locus_tag="VVN16_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14796..16559
                     /gene="aspS"
                     /locus_tag="VVN16_000760"
     CDS             14796..16559
                     /gene="aspS"
                     /locus_tag="VVN16_000760"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5572186.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            16537..17478
                     /locus_tag="VVN16_000761"
     CDS             16537..17478
                     /locus_tag="VVN16_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU5572187.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(17520..17702)
                     /locus_tag="VVN16_000762"
     CDS             complement(17520..17702)
                     /locus_tag="VVN16_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572188.1"
                     /translation="MRFNVGRFTSFSTEIESFPKIFLYSMKIKVQTRKLAAGCSKHCF
                     EVADRTDEVSNIYTAR"
     gene            complement(17769..18755)
                     /locus_tag="VVN16_000763"
     CDS             complement(17769..18755)
                     /locus_tag="VVN16_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5572189.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(18765..19565)
                     /locus_tag="VVN16_000764"
     CDS             complement(18765..19565)
                     /locus_tag="VVN16_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU5572190.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQTPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(19818..20660)
                     /locus_tag="VVN16_000765"
     CDS             complement(19818..20660)
                     /locus_tag="VVN16_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5572191.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(20662..21954)
                     /locus_tag="VVN16_000766"
     CDS             complement(20662..21954)
                     /locus_tag="VVN16_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5572192.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(22065..23336)
                     /locus_tag="VVN16_000767"
     CDS             complement(22065..23336)
                     /locus_tag="VVN16_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5572193.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGTKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            23886..25403
                     /gene="malQ"
                     /locus_tag="VVN16_000768"
     CDS             23886..25403
                     /gene="malQ"
                     /locus_tag="VVN16_000768"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU5572194.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            25429..27687
                     /gene="glgP"
                     /locus_tag="VVN16_000769"
     CDS             25429..27687
                     /gene="glgP"
                     /locus_tag="VVN16_000769"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU5572195.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(28732..29304)
                     /locus_tag="VVN16_000770"
     CDS             complement(28732..29304)
                     /locus_tag="VVN16_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572196.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT         8684 a         5258 c         6301 g         9092 t
ORIGIN      
        1 aagagaaatg tttgattata gagcactagt tattttgatg actttgatgg atcattgtga
       61 gctatcatta tatgaattat ctgttaaggt gagcttacct ataaaggaag tcaaagaagg
      121 aatagattat ttagtgcctt atctagctaa taaagggata gtgctggata aaaaacaagg
      181 tcgctatagt ttatcaaatc gtacgaagca gtctttgaca gatattatta agtcggatga
      241 attggtttta ccaaagtcga ctcgcttagc tttaatctat ctttacactt tttgccgatt
      301 agattttata tcgaataatc attaccaaga ctttttgaaa gtaagtaaga atacaacctt
      361 atctgatatt caatcattaa gaaagattat gttagataat gatttggagc tagggtacag
      421 tagagcaaaa ggttatactt tacacggttc agagtggaat aagcaccgct tagcttttca
      481 aatggttagt gagttgctgg aatcctccat agggatttgg ggtttggatt atgttttatc
      541 cagttggggg tattcattaa cttatgattt gattgatcag gtagttaaag attattatga
      601 gaagctacag ctagtaccta ttgttaatca attaaaagtt taccttttcg gtttagtatt
      661 cattctgtgt aggtatcaaa gggatgttga aagagtatgt ctttcagaga cattagtatc
      721 tcctgttatt caagatataa ccactatttt attggataca gtggttgatt tgggaattat
      781 agatacggtg ttttctgagg atgattatcg ctatatcacc gttttattat caagttgctt
      841 tgaaggtgaa gtagatgttg ctccggttta ctttaatcaa ttaacagagg ctattataag
      901 tagaatggaa gatatttctt tgttacattt taaacaaagg gaaggattga gagaaaatct
      961 tcgccgccac cttataccgg cttactatag attaaagttc ggcctaccta gttcaaatga
     1021 atatgtattg catgttaaag aacattatcc tgatttattt gaattagtca aagattcttt
     1081 gatgcccttg atggacgcta ttgacaatcc catacctgat agtgaaacag catattttgt
     1141 tatccatttc ggaggttatt taaagaaagc agatactttg cctcaaaaat ggtataaagc
     1201 agcaatcatt tgtcctaatg gtgttagttc ttcattgatg ttaaaagaga atctattagc
     1261 attatttcct caaattgagt ttataggaac ctcaaagatt gatgatttac aggtgaaagc
     1321 tagtagtgac tatgatatgg ttttttctac cataaaggtg gagacagaaa agccaaatta
     1381 tctagtttcg gttatgatga ctgaagagca agcaatacaa ctagtagaac tagtgttaaa
     1441 agattttccg aatttagagt atggagattt tgagattgaa caaatcctga atattgtcaa
     1501 aagatatgga attattacac aagaattgga attaagattg gcgttaaaga attatcttta
     1561 ccaaaaaaat gatagaaagg aaattgtacc agtgctagaa caacttatta ccaaagaaac
     1621 ctatcaggtt agttcgcaaa aattaggatg gaaggaggcg attcgtttag cagctaaacc
     1681 gcttttggat caagataaga taactgagaa ctaccctgag gcaatgattc aaaaagtaga
     1741 agagtttgga ccttttatta atttagggaa gggagtagca attcctcacg ctcggccaga
     1801 tgaaggtgtg aatgaaatag gaatgtcaat gttagttttg gaagaaccga tttatttatt
     1861 ggataatcca gaacaagagg taagattgtt gatttgtatt gcagccattg ataatgagag
     1921 tcatttaaag gctttgtcac atttaacaac aattttaaga gataaaaatc atgttcaaac
     1981 tttaatatca tcaaaaaact atgatgacat taaaatgata attaaacagg aggattagat
     2041 aatgttaaaa attggtacag cttgtggttc aggattaggt tcaagtttta tggtacagat
     2101 gaatattgaa tctgtattga gtgatttgaa tgtttcggat gtagaagttg aacattatga
     2161 tttaggtgga gcagatccaa atgcagctga tatttggatt gttggtcgtg atctagctga
     2221 ttcagctagt catcttggag atgttcgtat cttaaatagt attattgata tggatgaatt
     2281 acgagaatta attactaaac tttgtgaaga aaaaggactt atataggagg ctagcgtcat
     2341 gatgaagttc atattggata ttgttagtac accagctatt ttagtagctt taattgcaat
     2401 tttaggatta gttcttcaga agaagaaatt acctgatatt attaaaggtg gaattaagac
     2461 ctttgttggt ttcttagttg tatctggtgg tgcaggaatt gtacaaaatt ctttaaatcc
     2521 atttggtacc atgtttgagc atgcttttca tttatctggc gttgtgccga ataatgaagc
     2581 aattgtagct gtagctttaa caacatatgg ctcagctact gcaatgatta tgtttgcagg
     2641 catggtgttc aatatcttaa tcgctcgttt tactcgattt aaatatatct ttttaacagg
     2701 gcaccacact ctatatatgg cgtgtatgat tgcggtcatt ttatcagttg ctggctttac
     2761 tagcttgcct cttatcttac taggaggatt agcactcggt attattatga gtatttcccc
     2821 agcatttgtg caaaaatata tggttcaatt aactggaaat gacaaggtgg ctttaggtca
     2881 tttcagttct ttgggatatt ggttgagtgg ttttactggt agccttatcg gtgacaaatc
     2941 aaaatcaaca gaggacatta aatttccaaa gagtttagct tttttacgtg atagtactgt
     3001 tagtattact ttatccatgg cagttattta cattattgta gctatcttcg cagggtcaga
     3061 atatatagaa aaagaaatca gtagtggtac aagtggtcta gtttatgctt tacaattagc
     3121 aggtcaattt gcagcagggg tatttgttat tttagcaggt gttcgcctta ttttgggcga
     3181 aattgttcca gcctttaaag gtatttcaga gcgtcttgta cctaattcaa aacctgcttt
     3241 ggattgtccg attgtttata cttatgcacc caatgcagtt ctaattggat ttatctctag
     3301 ttttattggt ggtttagtaa gtatggcaat tatgattgct tcaggaacgg ttgttatctt
     3361 accaggtgtt gtgcctcatt tcttctgtgg agcgactgca ggtgtcattg ggaatgcatc
     3421 tggtggtgtt cgtggagcca ctattggagc atttttacaa ggtattttaa tcagttttct
     3481 tccagtcttt ttaatgccag ttttgggagg acttggtttc caaggatcaa ctttctcaga
     3541 tgcagatttt ggtctatcag gaattatttt aggaatgtta aatcaatttg gctcacaagc
     3601 aggcattgtg attggtcttg ttcttattct agcagttatg tttggagtat cctttattaa
     3661 aaagccatct gcaaaggagg aataagggat gattttaagt aaaaatagag aagatgggtt
     3721 aagaaaattt gcgactaaca tccgattaaa tactcttaga acattgaatc atcttggatt
     3781 cggacattac ggaggaagtc tgtctatcgt agaagtttta gcagtgcttt atggtgaaat
     3841 aatgccaatg actccagaaa tatttgcagc acgagataga gattatttca tattatcaaa
     3901 aggtcatgga ggaccagctc tatacagtac actctatttg aatggtttct ttgacaaaga
     3961 attcctatat tctttaaata caaatggaac caaattaccg tctcatcctg atagaaatct
     4021 aacgccaggc atagatatga caacgggctc tttaggacaa ggaattagtg ttgcaactgg
     4081 acttgcatat ggtcagagaa taagaaagag tcccttttat acttatgcta ttgttggaga
     4141 tggtgagtta aatgaaggac aatgttggga ggctatacag tttgcttctc atcaacagtt
     4201 atccaactta attgtatttg ttgatgataa caaaaaacaa ttagatggtt ttacaaagga
     4261 tatttgtaat ccaggtgatt tcgtagaaaa attttcagca tttggatttg aatccattag
     4321 ggtcaagggt tcagatatta gagaaattta tgaagggatt gtccaattaa aacagtcaaa
     4381 taattcatca cctaagtgca ttgtattaga tactattaaa ggtcaagggg ttcaagagct
     4441 ggaagaaatg aaatccaatc atcatcttcg ccctactgta gaggagaaac aaatgttaac
     4501 ttcagttgta gaaagattaa gtcaggaatt ggaggaaaca gaatgatgag aagtacgaaa
     4561 gaattacggc atgtatatag agacttcctt ttagaggcta atcaaagttc ttctgatata
     4621 gtagtcttag aagccgattt gtcaagttcg atggctactc ataatcttga aaaggacttt
     4681 ggagaccgtt atgtgaatgt tgggattatg gaagcagaaa tggtcggcct tgcagcaggc
     4741 ttgtctattc aggggtttag accttatctt catacatttg gtcctttttc ttcacgaagg
     4801 gtatttgatc aattatttat ttctcttgga tacgcacaat tggatgccac tgtgattgga
     4861 tcagatgcag gagtaactgc agaaatgaat ggtgggacac atatgccatt tgaagaaatt
     4921 ggattgttac gtttaattcc taaatcgatc atttttgaag caactgatga tatccaattt
     4981 cgtgaaatat tgaaccagac attagactta aaaggactaa aatatattcg aacaattaga
     5041 aaagctccag ttcctgtgta tcaaggtgga gaagattttt ctaaaggcta cattgagtta
     5101 agacatggtg aagatcttgt aatcgttgct tctggtataa tggttgctcc aagtattcga
     5161 gttgcagatg aactgtctaa attaggttat tcagtaggtg tgatagattt atttagaatc
     5221 aaaccgatac cagaacagat aaaaacaatg ttaagtggaa aaactatatt tactgtagaa
     5281 aaccacaatc agataggtgg aattggcagt tctttatgtg aattattctc tgatgatgga
     5341 ataacaaaaa ttcatcggat gggtgttcaa gaaagatttg gtcaagtagg gaaaatggat
     5401 tatcttctta atgagtatgg tttgagtgaa tcgaatataa aagagaaggt tctagaaatt
     5461 tataatgcaa gatagatgtt aaatacactt atctgaaaga atttctgtaa aaattacatt
     5521 acgttattta aaaaaagcgg attaaaaagc atattctggt catcagaaag tgcactaatc
     5581 cgctttgttg tttttaattt taaatatttc acatgcgtat agaattttct gaaaattcaa
     5641 gaattttatt ctgttcattg cctttaaatt gtgctataat agtaaaaact gaaatgggag
     5701 ggataagatg actgaattag ataaacgtca ccgcagtagc atttatgaca gcatggttaa
     5761 atcacctaac cgtgctatgc ttcgtgcgac tggtatgaca gataaggact ttgaaacatc
     5821 gattgtagga gtgatttcga cttgggcgga aaatacacca tgtaacattc acttgcatga
     5881 tttcgggaaa ctggctaaag aaggtgtcaa atctgcaggc gcttggcctg tacagtttgg
     5941 aaccattacc gtagcagacg ggatcgctat gggaacgcct ggtatgcgtt tctctctaac
     6001 atctcgtgac atcatcgcgg actccatcga ggcggctatg agtggtcaca acgtcgatgc
     6061 cttcgtcgct atcggtggct gtgacaagaa catgcctgga tctatgattg ctattgctaa
     6121 tatggatatc ccagctattt tcgcctatgg tggaactatt gcaccgggaa atcttgatgg
     6181 taaagatatc gacttggttt ctgtctttga aggtatcgga aaatggaacc acggtgacat
     6241 gacagctgag gacgtgaaac gtcttgaatg taatgcctgc cctggccctg gtggttgtgg
     6301 tggtatgtat actgctaata ccatggcaac tgctatcgaa gttctaggga tgagtttgcc
     6361 agggtcatcc tctcacccag ctgaatcagc tgataagaaa gaagatatcg aagcagcagg
     6421 acgtgctgtt gttaagatgt tggaacttgg tctcaaacca tcagatatct tgactcgtga
     6481 agcctttgaa gatgctatta ctgtaacgat ggctctcggt ggttctacaa acgccactct
     6541 tcacttgctc gccattgccc atgccgcaaa tgttgacttg tcacttgagg acttcaatac
     6601 gattcaagaa cgtgtgcctc acttggccga cttgaaacca tctggtcagt atgtcttcca
     6661 agacctctac gaagtcggtg gtgtccctgc ggttatgaag tacttgttgg caaatggttt
     6721 ccttcacgga gatcgcatca catgtactgg taagactgta gctgaaaact tggctgactt
     6781 tgcagacttg actccaggcc aaaaagttat catgccactt gaaaatccaa aacgtgcgga
     6841 tggtccgctt atcatcttga acgggaacct tgctcctgac ggtgcagttg ccaaggtatc
     6901 aggtgttaaa gtgcgtcgtc acgttgggcc agctaaggtc tttgactcag aagaagatgc
     6961 gattcaggcc gttctgacag atgaaatcgt tgatggcgat gtagtcgttg ttcgttttgt
     7021 tggacctaaa ggtggtcctg gtatgcctga gatgctgtca ctttcttcaa tgattgttgg
     7081 taaaggtcag ggagataagg tggccctctt gacggacgga cgtttctctg gtggtactta
     7141 tggtcttgtt gttggacata tcgctcctga agctcaggat ggtggaccaa ttgcctatct
     7201 ccgtaccggc gatatcgtta cggttgacca agataccaaa gaaatttcca tggccgtatc
     7261 cgaagaagaa cttgaaaaac gcaaggcaga aacaaccttg ccaccacttt acagccgtgg
     7321 tgtcctcggt aaatatgccc acatcgtatc atctgcttca cgcggagccg tgacagactt
     7381 ctggaatatg gacaagtcag gtaaaaaata aactcatact cgaaaatctc ttcaaatcac
     7441 gtcaacgtcg ccttgccgta ggtatggtta ctgacttcgt cagttctatc cacaacctca
     7501 aaacagtgtt ttgagctgac ttcgtcagtt ctatccataa cttcaaaaca gtgttttgag
     7561 caacctgcgg ctagttttct agtttgccct ttgattttca ttgagtatca aaaagcaagc
     7621 cattttcggc ttgctttttt catctttaaa agtgattggc ctgcttaacg aggtggtagt
     7681 ccaagggcaa tacggccata gcgactgatt cgtgtgattt tccaagcagg cgaccaggta
     7741 acttcaacct tgatatcctt gataccctcg atttgtttca gacctgccac gatttcaata
     7801 ggcaggcttt cggcgcaatc acaggcagta tcggtgaagg tcatgacaat cttgcagagc
     7861 cccgtttcat ccagattgat ttcataaatc agacctagat tgtaaacatc caattccaca
     7921 tctgtatcaa aaactttctc tagtttttcg atgatttggt cttgcaaggc caaagcacgg
     7981 tcattgattt tgatatcgtc tctcatagca gtccctccac gtgataaata tcctctattt
     8041 tctcataatt ctgacaattt ggcaagtttt tgatgaattt tctgaaaaat atgataaaat
     8101 gaagaaaata cggaatcaag gggaagtcta tggagcagat tggaaaagtc tttagacaat
     8161 tacgagagtc aagaaatatc tcactgagac aagtaaccgg aggacaattt tcgccgtcta
     8221 tgttgtcccg ttttgaaaca ggtcagagtg agctttcggt ggaaaagttt ctatttgctc
     8281 tggaaaatat atctgccagt gtggaggaga tcctctttct ggcgagaggt ttccagtatg
     8341 atacagattc tgagttgaga aaagaaatta cagatgtctt ggaaccaaag aatatagcac
     8401 ctcttgagga cttatatcgc agggagtatc aaaagcatgc ccattctcac aacaaacaga
     8461 aacatattct aaatgccatt attatcaagt cttatatgaa gagcatagat gaaagggtag
     8521 agttaacagc agaggaagga aaagtccttc atgattattt gttttctacc gagatctggg
     8581 gaatctatga actcaattta ttttcagtca gttctccatt tttatctgtt tctcttttta
     8641 ctagatatgt acgagaaatg gtacgtaaat ctgattttct aatggaaatg tctggcaatc
     8701 gaaacctttt tcatactata ctattgaatg gctttttagc tagtattgag tgtgaagaat
     8761 ttaccaatgc ctattatttt aaacgtgtta tcgaagagca tttctacaag gaaaatgaga
     8821 cctatttccg aattgtttat ctgtgggcag aaggtctcct tgatagcaag caaggtagag
     8881 tcaaggaagg ccagaaaaag atggaggatg ctgtccgtat ttttgagatg cttggctgta
     8941 ataaatctgc cgaatactat agaaatacga ccgaatgttg aatatctgca aaccaagaaa
     9001 aaatttgaag cgatagaact gttgccctct ctcaggtcat tacaaaagtt ctatcgtttt
     9061 tttgctcatt ttgtttgttg catatattca aaagaatcgt ttcgcaaatt cataagtgct
     9121 atactaaagc tattcttgat gtagaactta gaacaaggag ggagattatc tatgaaacta
     9181 ttgtttagaa atccagctta tagaattttg actttatcac gcttcttcaa tgcttttggt
     9241 gcttcgattt tcaacctggt ctttatcgtc tatgcatcga ccttgtcaca agcatctttt
     9301 gctgttgcta tggcgaatat tgtcatgatt cttccgacct tctttacagt ttttgcaggg
     9361 attcgggcag attacacgag ggacaaggtc aaatggatgg tctatagcgg tttgtttcag
     9421 gcggttctat tttttctagc agccctagtt gttcagcaag ctagtctctt tgccttttct
     9481 agcctgtgtt tgatcaatgt cattagtgat atcatcagtg attttgcagg tggcctgcgt
     9541 atgcctctta ttaaggaaaa agtagctgaa gatgatttga tggaggctta ttctttttca
     9601 cagttcatca cctatatttc agctattggt ggtcaagctt tcggagtctg gctcttagct
     9661 ctatcggtca acaatttttc cctcgttgcg ggaatcaatg cctgcttttt cctagtatca
     9721 gccactatcc tctttttagg aaaaagcaaa ttgagcctgt caatgtcatc tgctgatggt
     9781 gaaaacttaa aaaatgagaa gctttctatc aaagaccagt tcctaacaat ttaccgaaat
     9841 ttacgtctcg tttttcttaa aagtggacag aaaaactttg gttttatgct ctttgctgtc
     9901 ttgcttatta attccttggg tggcgctttg ggtggaattt ataatatctt ctttttgagc
     9961 cattctctct tgaatttttc ttatacagag gcactattta tcaatcaatt ctgtgtttta
    10021 gtagcaatca tcatcagtag ccttacgggc aatgattatt ttgggaagca gtccttgcct
    10081 agattgatga tgtgggcgac cgtaggactt agtctagttg gtttggctaa tttattcaat
    10141 caagtcgtgc ttggtttgct atttctcttc tttaccctgt atgtgtctgg taaagttcaa
    10201 ccaaagatta gtgccatgct catgaaaaat ctagctccag aggttctagc tcgtactagt
    10261 aattttttag gtctattgtt taccttatcc atacctgtgg gaacagcttg tttttcacta
    10321 gtagccgtat ggaatataca gttgacttgg atgctatttg ttggtctttc cttgctagct
    10381 atttttttga caattcttaa tctcaaaaat gatatctaaa tcctaatttt tttcttactg
    10441 ttttaatccc tttatttgtg gtaaaataag actattaagt ttaaagagga ttccctatga
    10501 aattacaaaa accaaaagga acgcaggata ttttacctgc tgaatctgct aagtggcagt
    10561 acgttgaggg ctttgcccgt gagattttca agcgctataa ctatgcagaa gtgcgcacgc
    10621 ctatttttga gcattacgag gttatcagtc gctctgtcgg agatacaacg gatatcgtaa
    10681 ccaaggaaat gtacgatttc tatgacaagg gtgaccgcca tattaccctc cgtccagaag
    10741 gaactgcgcc cgttgtccgt tcctatgtgg aaaataaact cttcgcccca gaagtgcaaa
    10801 agccaagcaa gttctactac atgggaccta tgttccgtta tgagcgtcca caggcagggc
    10861 gcttgcgcca attccaccag attggtgttg agtgttttgg ctctagcaat ccagctaccg
    10921 atgtggaaac aatcgttatg gcagcccatt ttttgaagga aatcggtatt caaggtgtca
    10981 aattgcacct caacactctt ggaaatcctg agagccgtgc agcctaccgc caagccttga
    11041 ttgactattt gaaaccgctc aaggagacct tgtctaagga tagccaacgt cgcttggagg
    11101 aaaatcctct tcgtgtcttg gactctaagg aaaaagaaga caaggtggca gtagagaatg
    11161 cgccgtctat cttggacttt cttgatgaag aaagccaaac tcattttgat gctgtgcgtc
    11221 agatgttgga aaatcttgga gtagattaca tcatcgatac caatatggtg cgtggtctgg
    11281 actactacaa ccacaccatt ttcgagttta tcacagagat tgagggcaat gacttgacag
    11341 tctgtgcggg tggtcgctac gatggtttgg ttgcttactt tggaggccct gaaactgctg
    11401 gatttggttt tgggcttggt gtagagcgcc tgcttctcat ccttgaaaaa caaggcgtgg
    11461 ccctccctat cgaaaacgcc ctagatgtct atatcgcagt cttgggtgat ggagcaaatg
    11521 tcaaagccct agaactagtc caagctcttc gccaacaagg tttcaaagca gagcgtgatt
    11581 acctcaaccg taagctcaaa gctcagttca agtcagcaga tgtctttgcg gctaagaccc
    11641 tcatcaccct aggagagagc gaagtcgaaa gcggacaagt gacggtcaag aacaaccaaa
    11701 cccgagaaga agtgcaagtg tcacttgaga caatcagcca aaacttctca gaaatctttg
    11761 aaaaactagg attttatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
    11821 ttgctcaaaa cactgttttg aggttgtaga tgaaactgac gaagtcagta accataccta
    11881 cggcaaggtg aagctgacgt ggtttgaaga tattttagaa gagtattaat gatagataaa
    11941 gtggtcagga acttactcct gacctttttc ttttgcaaaa tgacaaaaat cataaacttt
    12001 ttgacaacta tacttgtcaa aaagaaaaag atgtgttaca atgtaagcaa gttaagagaa
    12061 aagaagaaag gagctatgat gtgggtacta gggtttatac tatttctgat tttcttttat
    12121 tctaatgatt ctaaaaaaat caagaaacta gagaagaaaa tcaaaagact tgagcgaaaa
    12181 gagaaaggaa atgcagaaat gtcgagatta ttacaagaaa tgattggaaa ggaaccaatt
    12241 ataacgggag tgtatattgg gccagataac tgggaagttg tggatgttga tgaggaatgg
    12301 gtaaagctac gacgtgtaga taatacggga aaagaaaaat tcaagttgca acgtattgag
    12361 gatatccaaa ccgttgaatt tgacggagag taggtgtgta acatgcaact aaaaaatcgt
    12421 ctaaaagagc ttcgagctcg cgatggtctc aatcaaaccg acctagccaa actggcaggg
    12481 gtttccagac agaccattag cctactagaa cgggatgagt acaccccatc cattatcatt
    12541 gccctgaaaa tatctcaaat tttcaacgaa acagtcgaat cggtatttcg tttggaggag
    12601 gacgagtgat gaacaagtat aaagtgattt attatgtagt ggtcatagct ttattagtca
    12661 gcgtatttct actgattggg atggacctaa gctggtttaa tccctatcaa agcgaccaat
    12721 ttgtttgggt ctactttgct ctcatcccag taattgaatg gattgaaaag aaatccaaaa
    12781 atctagcaag tgaaaaagga gaatgaatat gaaaaagaaa cgtagattgt tatttttgat
    12841 gtctattgtc ttgggggggt tcttgggcat gtttgtaggg atgtttaagg cacgtgtcga
    12901 atcccacgaa attattttag atgtaaaagc cttgatgcca tggatatcag ctatttgttt
    12961 actgataggt ttcattagta tgtttttgac tttcaatttc ttaaagaaaa gtagaaaatt
    13021 tcattccttg tatcaagagg aaatggatga cgatctgaat gaaacctatt atgtgcaaat
    13081 gtatcggaat cttgagtttg gaaccattgc ttttaatatt acaggtgtag cgattccatt
    13141 ggctattttt atttcattaa gtgaggtgat tatattgcat acaaaccctc aaacattttt
    13201 cctttctttc ttactctttg tggtattctt agtcgctcaa aaatctcttt ttaaaaccat
    13261 tgcgattgtt cgtcagtttg atttggaatt tttcgctaca ccaaaggatg tcttgaacta
    13321 tataaattct tatgatgaag gggagcgtca ggctaatttg gaacagagtt ttcgaatttt
    13381 attccaatta caccaatatg tcttaccagc cttatatatt tttcttatta tcatttcttt
    13441 cttgacagga gagattcagt tactagcttt cttgcttgta ggagccatcc atgtttatat
    13501 caatgtgatg cagttaccta tggtaaaacg ttatttcaaa taaaggagtt atctatgaaa
    13561 ctacttaaaa accttggctg gtttcttcta gcccttctat cctttttctt tatctatggc
    13621 tttattcagg ggctcgcgac tgcgtcgctt gttttaggcg cttcacccta tgctgttact
    13681 ctactctatg tggtcttggc tggagtatat gtgtacggca tttacaaatg gtatcagaaa
    13741 gctcctgttt atattgagaa gagcggcttt aaccgattta tttggcttcc tgccttggtt
    13801 tggttcttat ctctggtcgt tcaattcttc ttgccagatg atccttcagt aaatcagcaa
    13861 atagcgacag acttgacctt gtctcaacca cttttctcat tctttgctgt ggttattttt
    13921 gctcctttga cggaagagat tgtttttaga gggatgttag cacgctatct ctttcctaag
    13981 caggacaata gtaaacgaac cctgattttt cttctggtat ctagtcttct atttgccttg
    14041 attcattttc caggtgatgt gcaacaattt tttgtctatt ttagccttgg ttttagtttg
    14101 ggtttggctt acattagcag aaaaggtctg gtctacagta tttctcttca cgctttgaat
    14161 aatttagtcg gctttttgat gattctcatg ctataataga gtcaggaggt cacatgaaac
    14221 gagtaatttt agtagcagtg attcaggcag tcgttctatt tttcatcatt ggagggctag
    14281 cttatgcctt caaaggcgat ttcttctata actatctagc agttgtcttt gctcctattg
    14341 caggtgtact gcgttttggg acggcttaca taacggaaat tgtcttgcct cgaaaggcag
    14401 ccgaaatcgc tgaaaagcgt aaagcaggca aaaattcaaa ataaaagagt ccggtgaagt
    14461 tcatcggatt tttgtattat actcaatgaa aatcaaagag caaactagga agctaaccgc
    14521 aggttgctca aaacactgtt ttgaggttgt ggatgaaact gacgaagtca gctcaaaaca
    14581 tggttttgag gttgtagatg aaactgacgg agtcagtaac catacctacg gtaaggcgac
    14641 gctgacgtgg tttgaagaga ttttcgaaga gtattagcgc taatttttag ggctttcatc
    14701 tgacttttaa agactggcaa acttttctga tgattttcat gaagagcctg cgcttttatg
    14761 gtaaaatagt aacagaataa aagaggagag aaataatgaa acgtagtatg tatgctggtc
    14821 gtgttcgtga ggaacacatc ggacaagaaa taaccttgaa aggatgggtt ggccgtcgtc
    14881 gtgaccttgg tggtttgatc tttatcgatc ttcgtgaccg tgaaggaatc atgcagttgg
    14941 ttatcaaccc tgaaaaagta tctgcagagg ttatggcaac agctgaaagc cttcgtagcg
    15001 aatttgttat tgaggtgact ggtcaggtcg ctgcgcgtga gcaagccaat gataagttgc
    15061 caactggtgc ggttgagtta aacgtgacag ctcttattgt gcttaacaca gctaagacaa
    15121 caccatttga gattaaggat ggcattgagg caaatgacga tacacgtttg cgttaccgtt
    15181 accttgacct tcgtcgtcca gaaatgttgg aaaatcttaa acttcgtgcc aaggtgaccc
    15241 actctatccg caactacttg gatgagttgg agtttatcga cgtggagaca ccattccttt
    15301 ctaagtcaac gcctgaaggg gcgcgtgatt atttagtgcc gtctcgtgtt aataaggggc
    15361 atttttacgc tcttcctcaa agtccacaaa tcacgaaaca gctcttgatg aatgctggtt
    15421 ttgaccgtta ctaccaaatc gttaaatgtt tccgtgacga agacttgcgt ggagaccgcc
    15481 agcctgaatt tactcaggtc gacttggaaa cgtccttcct tactgagcaa gaaatccaag
    15541 atattacaga aggcttgatc gcgcgcgtga tgaaggaaac aaaaggcatc gaagtaacgc
    15601 taccgttccc tcgtgtgaaa tacgatgatg ctatggctct ttacggttct gacaagccag
    15661 atactcgttt tgacatgttg cttcaggact tgacagaagt ggtcaaaggt gtagacttta
    15721 aagtcttttc agaagcacct gctgtaaaag cgattgtggt caaaggagct gcggacaact
    15781 attcacgtaa agacatcgac aagatgacgg aagtagccaa acagtatggt gccaaaggtc
    15841 ttgcttgggt taaggtggtt gatggagaat taaacggacc agttgccaag ttcttgactg
    15901 gtatccaaga agaattgaca acagcgcttg ctcttgaaga taaggacttg gttctctttg
    15961 tggcggatac gcttgaagtg gctaatgcaa cactgggtgc ccttcgtgga cgtattgcta
    16021 aagagcttgg cttgattgat aatgataagt tcaacttcct ttgggtggtt gactggccga
    16081 tgtttgaatg gtctgaagaa gaaggccgct acatgagcgc ccaccatcct ttcacccttc
    16141 cacaggaaga gactgctcac gaattagaag gtgatttggc taaggttcgt gccattgctt
    16201 acgatatcgt cttgaacggt tatgagcttg gtggtggtag ccttcgtatc aaccaaaaag
    16261 accttcaaga acgcatgttc aaggctcttg gtttctcagc tgaagaagcc aatgaccagt
    16321 ttggtttcct tcttgaagcc atggactatg gtttcccacc acacggtggt ttggctatcg
    16381 ggcttgaccg ttttgtcatg ttgcttgctg gagaagaaaa tatccgtgaa gtcattgcct
    16441 ttcctaagaa caacaaggca actgacccaa tgacacaagc tccatcaaca gtcgctctca
    16501 aacaactaga ggaactcagc ttacaagtag aagaagatga aacaagcaaa acgaattaag
    16561 cggtggcgct attatctgcg ccgctttgct tatcagataa aaattttacg tgtcttacaa
    16621 agtatctctc gagaaaagta tgatgagaag atttcggcct ctctggtcta tggtttttta
    16681 tcagcagtag cagttaattt ctttttccaa ccagggcatg tgtattcgag tggtgcaaca
    16741 ggtctggcac agattatctc tgccttgagt aatcactggt ttggttttca tattccgatt
    16801 tcgctaagct tctacgccat taacttccct ttgatggtct tagcttggta tcagattggc
    16861 cataagttca ccgtctttac ctttatcacg gtatctatga gttccttctt tatccagttt
    16921 gtccctgtgg caaccttgac ggaggatccc attatcaatt ccctttttgg gggtgttgtt
    16981 atgggtttgg ggattggttt tgctcttcga aacaatatct ccagtggtgg gacggatatc
    17041 gtcagcctga ctattcgcaa gaaaacgggt aagaatgtcg gtagtatttc tttcttggta
    17101 aatggaacta tcatgctgat agcaggtttg acctttggtt ggaaatacgc tctttattct
    17161 atgattacca tctttgtctc tagccgtgtg acagacgcag tctttactaa gcaaaagcgt
    17221 atgcaggcca tgattgtgac aaatcatcca gagaaggtaa ttgaaaaaat ccataaaaaa
    17281 ttgcaccgcg gagcaaccat gatccacgat gcagaaggaa cctataatca cgagagaaag
    17341 gcagttttaa tcactgtcat tacacgtgca gagtttaatg aatttaaaca gattatgaca
    17401 caagtggatc caagctcctt tgtctctgtc tcggaaaatg ttcatattct aggaagattt
    17461 gttgagatag ataattagtg attaatactc ttcgaaaatc tcttcaaacc acgtcagctt
    17521 caccttgccg tatatatgtt actgacttcg tcagttctat ctgcaacctc aaaacagtgt
    17581 tttgagcaac ctgcggctag cttcctagtt tgcactttga ttttcattga gtataaaaag
    17641 atcttgggaa aagactcaat ttcagtagaa aaggaagtaa atcttcccac attaaaacgc
    17701 atagtatcaa gttttttcaa tacctgatac tatgcgtttt tgcttttaac gactttttgc
    17761 ccagtctctt atttttcgat aattttgtgg gcgatcaatt gacggtaaca aatttgttta
    17821 ttgtttttat tatcattaat aatctggaga atagtttcaa gggaaacacg accaagctct
    17881 aaactattga tatcgacata ggctgccaag ttgagtttgg gattaaccga gtcaaagctg
    17941 agaacaggga catccagctg gtgtttggca atatagttac aaacaccttc agctaggagg
    18001 ctatcggttg tgatgatagc atcaatttgt ggatcgtgct tgaataatcg cttgctaaat
    18061 ttatagccct tttcttccag aaactcgtcg gcaaagtaga tgcgattgtt gtcagtggta
    18121 agtttgtaat gtttaagcgc ctgttcatag cctgttaaac ggtctttggt cacgaagagc
    18181 tttttacttc ctccgataaa ggcaatgcgt ttgcagcctt ttttgatgaa atattcagtc
    18241 gcatcaaaac cagcttgaac attgtcgttg tcgacaagtg ggatgaaagg agatagagat
    18301 ttacctaaga taaggaaggg gaactgttct tctgcgacga gttttacgag agggtcttct
    18361 tcttgggcat agagaaagat tagcccatct acacgcttgc cgtagaccat ttgtgaaata
    18421 gcgttgagac gctccttctc atctttccct gttgctatct gaatggcata gtggttttca
    18481 gatgcgactt gagagatgcc acgtagaacc gatggaaaga aaggattctg gtagaaggcg
    18541 tctgagtcat caggaagaac taatccgata acctgagtat agctgcttac caagctacga
    18601 gcgttgaggt ttgggtggta gttgagttcc ttcatagctt tgcgaacgcg tttttttgtt
    18661 tcgtcgctaa tggttgattt attttgaata acacgggtta cggttgaagg cgaaacacca
    18721 gcagccttgg ccacgtcttt aatcgtaacg ggcataaaaa tctcctattt atggtaattt
    18781 ggatcacgga aatgtgtttt ataaaagata gtgaccagat acagaacaaa aagaatattt
    18841 tgtacagtaa tcagggttgt catattttgg ccaaataatc ccaaaataag tgtaatcaga
    18901 gtcggcaatc ctaaacagtt caagataaag tgatagcact ctttaaaggt tctaaatgaa
    18961 aagaggcgtg atttcttagt gatataaagg agaagactag ctcctagaga gacgataaag
    19021 aaattcaaac caaagaggaa gctcgcaccg agaactagga agaggctgat atagacacga
    19081 ttttgttggt accagtcttt agaaattgct tgggtcaagc tgtctttgct tttgaaactc
    19141 tcagtctgaa tggctcggta agagatgcgg gtcagctcct tgctttcctt gctgatgact
    19201 agctcatttg tatcgaaatg cagttgcaag tccttaggta attccttgat ttgacttgga
    19261 ccaatcacaa cagaaggggt ttgactagct gttccagtat aagttaatgt gccatcgaca
    19321 attgtagcat gttcagagag atcctggaca acctcatttg tcagaggttc atagacatta
    19381 tcgataaaag tttctagcgg ataggtctcc tgggagctgt tttggatagc aatgggtacc
    19441 atagacaagc tgataaggaa gatactggta aagagaagtt gaaaccagtt gagctcgaaa
    19501 cgtttggaca ggggcttacg aaacccccaa atacttgaaa aataggaaaa tggatatgga
    19561 agcataggta tctttctaaa aggtgttttc tatatgagta ttattatata gagaaatgcg
    19621 ctttatttca agtctaggag aaaaattgct tgctacaagg atattattta cagtgacaat
    19681 tgctaataat caatgaaaat caaagtgcaa actagccaca ggttgctcaa agcacaactt
    19741 tgaggttgga gataaagctg acgtggtttg aagagatttt cgaagagtat aaaatgaaaa
    19801 agggtggcgg ggataaatta tcccttgtcg ccaccacttg taagtcctga aacaaagttc
    19861 ttttgtagga agaagaagag aatacagatt ggaagggcga tgaggatagc acctgctgag
    19921 aagtaggcaa tcttcaagtt tttcgcattg ttaacgaagg tttggagacc tacggcaaca
    19981 gtaaagtatt ctttctcacg aagcaagaaa ctagagagga tgtagtcccc gaaaggtccc
    20041 atgaaggccc agagagcttg tacggcaacc attgggcgaa caagtggaag aacaatttgc
    20101 cagaagcggc ggaagtgtcc tgcaccgtct agttttgcag attcgtctaa agacattggc
    20161 actgtatcga agtagccttt catgagccaa gcattcatag ggataccacc accaacgtag
    20221 aggaagatga ggaaccagtt gtggttaagg gcgttcaaca taagcgccat aacgaagaag
    20281 gctgtcaaag cggccatagt tggcaccatt tggatgatca agaagaagac caaactttgt
    20341 ttacgagcca agaagttgta acggctgtaa gcataaccag caagtacgat gatacttgtt
    20401 tgaacagcca tggtaattaa ggcgataatc aaagtgttga ggtaccaagt accgtacaag
    20461 gtttcagtga agaggccttt aaagttatca aaattgaggt cgatattagt atctagttta
    20521 aaggctgaga cgttacctgc tttaaaggct gacataatgg taatcaacag tggatagata
    20581 attacaattg atagaccaat caggtaaagg taagtaaggc tttgagtcag tctacgtttg
    20641 agtttaattg agttattcat cttagacgtc ctccatatca aatgcgtgta gtttcttgaa
    20701 tgcgatcata gagattgaga tgacaatgat agagataatc aaggtaacag ctgccgccat
    20761 tgagtattga ggagatgtac ctgttgtcaa acggtagatc catgagatca agatatcggt
    20821 tgaaccagct ccacctccga cactaccagg tcctccacca ttgaagaggt acatgataga
    20881 gaagttgtta aagttgaagg tgtattggct aatcaaagta ggtgccgcaa cagccaaaat
    20941 cattgggaaa gtgatgttgc ggaatttttg ccaagcgttg gcaccgtcaa tataagctgc
    21001 ttcgtaaagg tcgttaggaa tagattgcaa gatacccaaa gtcaaaacgt agatgtatgg
    21061 gaatccgagc caaccttgca tcataatcaa ggcaatctta gtccaagttg ggtctgtttt
    21121 ccaaggaata agagctccat caaggaaagg aaggaattta gccaagattg gcaatacttg
    21181 agtgttgata gcaccgacac tatcgttaaa catgtttgag aatgtcaaga tagtgatgaa
    21241 ggctgggaca gcccaaggaa gaaggaaaat aacaccaaag atacgttttc ctttgataaa
    21301 tggttggttg gcaatgatag ctgtgaagat accaattacg atttgtaaag tagaagctgc
    21361 caaagcccaa atgatagtcc aagaaagaac agaaccaaag gcagaacgga aggtactcaa
    21421 gctccaaatg tttgtaaagt tggtcaaacc aacccagtcc aacaacttgt ttggtggcaa
    21481 gtgttggaag tcgtagttgg taaaggcgat catcaaggtt acgataactg ggaagataat
    21541 cgcgaaggtc atggcaacat aagatggaat gatcaagagg taagggaagc cattttcata
    21601 aatccctttg atcatgtctt tgagtgtgcg tggaactgga attccattgt taatgcgttt
    21661 tgcaatcgta tgtgcatctt tgatatttga gaaataaaag agtacataaa cgattacaaa
    21721 gattagatgg aaggcaccac gaatcagcat aaagagggaa ttatcacgac ctggtttgtc
    21781 accaagagtg atgaggttgc tcaattcagg ggttgcaagt gctaggaagt aaaggacaaa
    21841 tacgatggtt acaccaagga agataaaacc tttggctttt tgtttattgt aaatctgtcc
    21901 taacccagga atgatagaca gcagggctgc tttactaggt tgttgctttt ccatattaac
    21961 tcctttcata ggatactact tttttataag aaagctaaat tatttgtgtc tctattgata
    22021 aattcaaagg gggatttgat ttccaccccc cttgaacaaa ttttttattc accaaatttt
    22081 tgtttgattg tttctttgat caatgttaca gcatcgttag cagctgtttt agcatctttt
    22141 tgaccactta cagcatcaaa gagcatattt ttcgctggat cccaaactgc agacatttga
    22201 gagatgtttg gcagtggttg agtgttcttg aactgtttga taacagctgt tgtcaactca
    22261 tcgtttttac cttcagcgta tgaacgagcc tcagtattag ctgggatttc gttagtctta
    22321 tcatataata ctttttgttg ttcagttgca acaaggaagt ctacaaattt ttgagaagct
    22381 tcaaggttct taacggcttg aggaatgacc caagctttac caccaccgaa tgcagcatat
    22441 tcttttccat ttggaagagt tgggatagtt gcaactccgt agtttacttt agcatcttta
    22501 aaggcttgag ctttccaagg tccgtcgatg atagcagctg ttttaccttc ttggaattga
    22561 gtttggatta agtttccagc accttctgta tcttgcatac ctttaggcca tttttcgtac
    22621 caagatttag cgtaattgat acctacgata gaaccgtcgt ttgcaagacc gatgtcttta
    22681 gcgtctttac cgttttggcc aaagacgtaa gcaccgttac cggcaagaag tccatatgta
    22741 tagtagaagt ttgtccagtc agctaggaag gcagtagttt taccatcttc accagcgaat
    22801 gcgtatttgc tatctttagc aaggttttcc aagtcagcaa atgtttttgg agcatctttc
    22861 accaagtctt tgttgtagta cataacaagt gactcgataa cggcaggagc accgtaaact
    22921 ttaccattag cagctgttac aagagattta gttgtgtcgt ctgttttagt accatcgctc
    22981 aatttcactt ctgaaagttg tccgtcagaa ccaaggctac ctacacggtc gtatggagcc
    23041 atcataacat cagggacatt accagattgg ttgtcaagag aaagtttatc aagacctcct
    23101 agagcatcac cagttttaag agtgactttt actccagctt ctttttcata agctttagca
    23161 acctcttcaa tatagctctt atatccctcg tctacatata cagtgagttc tttgacttca
    23221 gatgaaccag aatcagcagg cttatcagca gttttgcttc cgcaagctac caaaagcaag
    23281 ctagcaagtg taacagttcc aagcaccgca gtgctcttca taaatttaga tgacatagtg
    23341 tattcctccc aaagaatagc aagttttatt gataaggaaa cgcaaacgtt ttcctttatg
    23401 agcttagtat agcacaaata gaaaacggtt gcaagtattt tttgtaaaaa ttttaaaaaa
    23461 tttttaagct tgatttgata gcataatttt gcattttaat agaagaaaag tatgaaatag
    23521 ataagaatac agtattttta ataaaaatat atggaatctg tctaaagagg gaaatcagga
    23581 ggggctacct ccctggttct aatccctatc tattttctat agacgtttgt gctttgttgc
    23641 atataaattc aagctcccta aggaggtttt gtactttgta gactgtctcg tgagagtcaa
    23701 accttaggat tagatagttt taagttatct ttgctaaaat gagttagtct ccttaaaaat
    23761 aatcaaaaaa gttttttaaa aacgcttgca attatgcttg aaaaggagta tacttataag
    23821 taacgcaaac gtttgcgtct gaaaaatacg caacgttcca ttattttaac acacgaggtg
    23881 ctattatgaa aaaacgtcaa agtggtgtgt tgatgcacat ctcttctctt ccaggagctt
    23941 acggaatcgg atcatttggt caaagtgctt acgacttcgt tgatttcttg gtccgtacaa
    24001 aacaacgtta ctggcaaatc cttccattag gagcaactag ttacggggat tctccttacc
    24061 aatctttctc agccttcgca ggaaacactc attttatcga tttagatatc ttggtggagc
    24121 aaggtttgtt ggaagcaagt gaccttgaag gagttgactt tggtagcgat gcgtctgaag
    24181 ttgactatgc taaaatctac tatgcacgtc gtcctctttt agaaaaagcg gtgaaacgtt
    24241 tctttgaagt cggagatgtt aaagattttg agaaatttgc tcaagacaac caatcatggc
    24301 ttgagctctt tgctgagtat atggctatca aagagtattt tgacaatctt gcttggactg
    24361 aatggccaga tgcagatgct cgtgctcgta aagcttcagc acttgaaagc tatcgtgagc
    24421 aattggcaga caagttggtt taccaccgtg tgactcaata cttcttcttc caacaatggt
    24481 tgaaattgaa agcttacgct aacgacaacc acatcgaaat cgttggggac atgccaatct
    24541 acgtagcgga agattcaagt gatatgtggg caaatccaca tctcttcaaa acagatgtca
    24601 atggtaaggc tacttgtatc gcaggatgcc caccagatga gttttctgta actggtcagc
    24661 tttggggtaa cccaatctat gactgggaag caatggacaa agacggctac aaatggtgga
    24721 ttgaacgctt gcgtgaaagc ttcaaaatct acgatatcgt tcgtatcgac cacttccgtg
    24781 gcttcgaatc ttattgggaa atccctgctg gttccgatac agcagcacct ggtgagtggg
    24841 tgaaaggtcc aggctacaag ctttttgcag ccgttaagga agaacttggt gagctaaaca
    24901 tcatcgcaga agaccttggc ttcatgacag atgaagtgat cgaattgcgt gaacgtactg
    24961 gcttcccagg aatgaagatt cttcaatttg ccttcaaccc agaagacgaa agcattgata
    25021 gcccacactt ggcacctgct aactcagtta tgtacacagg aacacacgat aacaatacgg
    25081 ttcttggttg gtaccgtaat gagattgatg atgcgactcg tgagtacatg gctcgttaca
    25141 cgaaccgtaa agaatacgaa acagtggtac acgctatgct tcgtacagta ttttcatcag
    25201 ttagctttat ggcaattgca actatgcaag atttactaga attggatgag gcagctcgta
    25261 tgaacttccc atctaccctt ggtggaaact ggtcttggcg tatgactgaa gatcaattga
    25321 caccagctgt cgaggaaggt ttgcttgact tgacaacaat ttatcgccga attaatgaaa
    25381 atttggtaga tttaaagaaa taagacaata atcaggagac aactaaacat gttatcacta
    25441 caagaatttg tacaaaatcg ttacaataaa accattgcag aatgtagcaa tgaagagctt
    25501 taccttgctc ttcttaacta cagcaagctt gcaagcagcc aaaaaccagt caacactggt
    25561 aagaaaaaag tttactacat ctcagctgag ttcttgattg gtaaactctt gtcaaacaac
    25621 ttgattaacc ttggtcttta cgatgatgtt aaaaaagaac ttgcagctgc aggtaaagac
    25681 ttgatcgaag ttgaagaagt tgaattggaa ccatctcttg gtaatggtgg tttgggacgt
    25741 ttggctgcct gctttatcga ctcaattgct actcttggtt tgaatggtga cggtgttggt
    25801 cttaactacc actttggtct tttccaacaa gttcttaaaa acaaccaaca agaaacaatt
    25861 ccaaatgcat ggttgacaga gcaaaactgg ttggttcgct caagccgtag ctaccaagta
    25921 ccatttgcag actttacttt gacatcaact ctttacgata ttgatgttac tggttatgaa
    25981 acagcgacta aaaaccgctt gcgtttgttt gacttggatt cagttgattc ttctattatt
    26041 aaagatggta tcaactttga caagacagat atcgctcgca acttgactct cttcctttac
    26101 ccagatgata gtgaccgtca aggtgaattg ctccgtatct tccaacaata cttcatggtt
    26161 tcaaacggtg cgcaattgat catcgacgaa gcaatcgaaa aaggaagcaa cttgcatgac
    26221 cttgctgact acgcagttgt ccaaatcaac gatactcacc catcaatggt gattcctgaa
    26281 ttgattcgtc ttttaactgc acgtggtatc gagcttgacg aagcaatctc aattgttcgt
    26341 agcatgactg cctacactaa ccacacaatc cttgctgaag cgcttgaaaa atggcctctt
    26401 gaattcttgc aagaagtggt tcctcacttg gtaccaatca tcgaagaatt ggaccgtcgt
    26461 gtgaaggcag agtacaaaga tccagctgtt caaatcatcg atgagagcgg acgtgttcac
    26521 atggctcaca tggatatcca ctacggatac agtgttaacg gggttgcagc actccatact
    26581 gaaatcttga aaaattctga gttgaaagcc ttctacgacc tttacccaga aaagttcaac
    26641 aacaaaacaa acggtatcac tttccgtcgt tggcttatgc atgctaaccc aagattatct
    26701 cactacttgg atgagattct tggagatggt tggcaccatg aagcagatga gcttgaaaaa
    26761 cttttgtctt atgaagacaa agcagctgtc aaagaaaaat tggaaagcat caaggctcac
    26821 aacaaacgta aattggctcg tcacttgaaa gaacaccaag gtgtggaaat caatccaaat
    26881 tctatctttg atatccaaat caaacgtctt cacgagtaca aacgccaaca aatgaacgct
    26941 ttgtacgtga tccacaaata ccttgacatc aaagctggta acatccctgc tcgtccaatc
    27001 acaatcttct ttggtggtaa agcagctcca gcctacacaa tcgctcaaga cattatccat
    27061 ttaatccttt gcatgtcaga agttattgct aacgatccag cagtagctcc acacttgcaa
    27121 gtagttatgg ttgaaaacta caacgttact gcagcaagtt tccttatccc agcatgtgat
    27181 atctcagaac aaatctcact tgcttctaaa gaagcttcag gtactggtaa catgaaattc
    27241 atgttgaacg gagctttgac tcttggtact atggacggag ctaacgtgga aatcgctgag
    27301 ttggttggtg acgaaaacat ctacatcttt ggtgaagatt cagaaactgt tatcgacctt
    27361 tacgcaaaag cagcatataa atcaagcgaa ttctacgctc gtgaagctat caaaccattg
    27421 gttgacttca tcgttagtga tgcagttctt gcagctggaa acaaagagcg cttggaacgt
    27481 ctttacaatg aattgatcaa caaagactgg ttcatgactc ttcttgattt ggaagactac
    27541 atcaaagtca aagagcaaat gcttgctgac tacgaagacc gtgacgcatg gttggataaa
    27601 gtcatcgtta acatttctaa agcaggattc ttctcatctg accgtacaat cgctcagtat
    27661 aatgaagaca tctggcactt gaactaatac tcttcgaaaa tctcttcaaa ccacgtcagc
    27721 tttatctgca acctcaaagc ggtgctttga gcaacctgcg gctagcttcc tagtttgctc
    27781 tttgattttc attgagtata agatacaaat ttatactaat acattttgta aaaaagtgag
    27841 tttcgattga aattcgcttt tttaaatgct gtggatttgg gtcaatcttg tctaaaaata
    27901 gggaaatcct agatacagtg aaggctttaa atgctggttt ttactgtctt cagccttata
    27961 ttttttcgta gttggttacc tcatatctct tgtattcgct tacataaagt attataatat
    28021 aattgtagga aagaaggtgt ttttatgata tacacactta aattggtgtt gtttattacc
    28081 tttcttgtaa taagcttgtt acctgataag atttttggaa aaaataaaaa aatttggaaa
    28141 atagtttttg caatattgac ggcagtggca gcattgtcat ttatgtacta agttatttta
    28201 agaatgtagg gaaataaacc ctacattctt tttagttttt tctgttttct aaattctatt
    28261 tatccaagcg attcaacatt tcttgcttct tcgcttcaag ttctgcacgc ttttcttcga
    28321 tttcggcatg ttttttctcg agttcagaac aacttgcacc attgctaaat tcttttcgcc
    28381 atcaggagat agggtgagtc gacatgtcta ttactcaccc aaagcagtcc tacaaagcag
    28441 gaattttctg ttactttttt ggaaatagta acgtttatac agctttgaca cttcgtatca
    28501 aagcgccaaa cacactccga gggatttaca gaaagcagaa aaggaatgat ctggtataag
    28561 atcattcctt ttctctcttt ttctttaagt aattatatac aatgtacgac gaagtcgtca
    28621 ttgcaatgct gatccaccac ctaaagggaa ctttaaacaa cattgataag ataaagaata
    28681 taaacaacga aaatacgtta tacccaatta attttattgt atatctcatg attaaaagtt
    28741 aatccttccg ttgttaggaa tggcatcatt tttatcccat aattgtgcta aataagtccc
    28801 cggtgataat aaattcatag cgaattctaa agcaacatca tttacaaacc aactacctag
    28861 atatctagaa attgctgaac gaatagcact ttttgctgca tgttttcctt ttactttaat
    28921 tagatttgca aggcctgcag tagttcctcc taatgctaaa gctattgcag tatctaatag
    28981 agcacccatt tgattaactg taataccttg ccaagctgct ctaaatggag agtatgtagg
    29041 tgggattgta taatcgcctt gtaattgtcg gttaattact tctttgatcc attgttgtga
    29101 gacgtctgga tgaaaagatt ggatttcgtt tgcaagtgta ttgatttgtt cttctgttag
    29161 agaagtgaca ggttgaagtt ccatatttgt ttcaatttgt gatacttgtt cagaagcgta
    29221 tacagctgaa acacttggaa tcgctgatac aattaacaca attgacgtca aaaaaaccga
    29281 aataaatttc attaatttgt tcatgagctt ttctcctttt tatttgtatc tgctt
//
LOCUS       DAVHIT010000021        28500 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVHIT010000021 DAVHIT010000000
VERSION     DAVHIT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.021"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            219..665
                     /locus_tag="VVN16_000771"
     CDS             219..665
                     /locus_tag="VVN16_000771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572197.1"
                     /translation="MADRTDEVSSKHRFEVADRTDEVSSKHRFEVADRTDEVSSKHCF
                     EVADRTDEVSSKHCFEVVDRTDEVSSKHCFEVADRTDEVSSKHCFEVADRTDEVSSKH
                     CFEVVDRTDEVSSKHCFEVVDRTDEVSNHTYGKAKLTWFKEIFEKY"
     gene            complement(987..1968)
                     /locus_tag="VVN16_000772"
                     /pseudo
     CDS             complement(987..1968)
                     /locus_tag="VVN16_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
     gene            complement(2284..4299)
                     /gene="recG"
                     /locus_tag="VVN16_000773"
     CDS             complement(2284..4299)
                     /gene="recG"
                     /locus_tag="VVN16_000773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU5572198.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKTEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(4318..5421)
                     /locus_tag="VVN16_000774"
     CDS             complement(4318..5421)
                     /locus_tag="VVN16_000774"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5572199.1"
                     /translation="MKASPHRPTKVLIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEVTATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(5411..5779)
                     /locus_tag="VVN16_000775"
     CDS             complement(5411..5779)
                     /locus_tag="VVN16_000775"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5572200.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5818..6849)
                     /locus_tag="VVN16_000776"
     CDS             complement(5818..6849)
                     /locus_tag="VVN16_000776"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5572201.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6851..7882)
                     /locus_tag="VVN16_000777"
     CDS             complement(6851..7882)
                     /locus_tag="VVN16_000777"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5572202.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7963..10476)
                     /gene="secA"
                     /locus_tag="VVN16_000778"
     CDS             complement(7963..10476)
                     /gene="secA"
                     /locus_tag="VVN16_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5572203.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10622..10909)
                     /locus_tag="VVN16_000779"
     CDS             complement(10622..10909)
                     /locus_tag="VVN16_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572204.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLVFGILSILMAFLSKKVSLFVFGLICCLSLWINLFILFALLPIFGN"
     gene            complement(10937..11569)
                     /locus_tag="VVN16_000780"
     CDS             complement(10937..11569)
                     /locus_tag="VVN16_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5572205.1"
                     /translation="MKLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(11573..12214)
                     /locus_tag="VVN16_000781"
     CDS             complement(11573..12214)
                     /locus_tag="VVN16_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572206.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(12207..12998)
                     /locus_tag="VVN16_000782"
     CDS             complement(12207..12998)
                     /locus_tag="VVN16_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572207.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(13183..13518)
                     /locus_tag="VVN16_000783"
     CDS             complement(13183..13518)
                     /locus_tag="VVN16_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572208.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     SLFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(13658..13993)
                     /locus_tag="VVN16_000784"
     CDS             complement(13658..13993)
                     /locus_tag="VVN16_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572209.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(14081..15391)
                     /gene="der"
                     /locus_tag="VVN16_000785"
     CDS             complement(14081..15391)
                     /gene="der"
                     /locus_tag="VVN16_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5572210.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFIIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(15405..16118)
                     /locus_tag="VVN16_000786"
     CDS             complement(15405..16118)
                     /locus_tag="VVN16_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU5572211.1"
                     /translation="MTEIIKLMKAHTSVRRFKEQVLPQEDLTEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(16115..17011)
                     /gene="dnaI"
                     /locus_tag="VVN16_000787"
     CDS             complement(16115..17011)
                     /gene="dnaI"
                     /locus_tag="VVN16_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU5572212.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIATEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(17012..18181)
                     /locus_tag="VVN16_000788"
     CDS             complement(17012..18181)
                     /locus_tag="VVN16_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU5572213.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHTVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(18182..18655)
                     /gene="nrdR"
                     /locus_tag="VVN16_000789"
     CDS             complement(18182..18655)
                     /gene="nrdR"
                     /locus_tag="VVN16_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU5572214.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18795..19160
                     /locus_tag="VVN16_000790"
     CDS             18795..19160
                     /locus_tag="VVN16_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5572215.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            19165..19860
                     /locus_tag="VVN16_000791"
     CDS             19165..19860
                     /locus_tag="VVN16_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572216.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19877..20644
                     /locus_tag="VVN16_000792"
     CDS             19877..20644
                     /locus_tag="VVN16_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572217.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(21056..22255)
                     /locus_tag="VVN16_000793"
     CDS             complement(21056..22255)
                     /locus_tag="VVN16_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572218.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(22252..23145)
                     /locus_tag="VVN16_000794"
     CDS             complement(22252..23145)
                     /locus_tag="VVN16_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572219.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            23262..23342
                     /locus_tag="VVN16_000795"
                     /pseudo
     CDS             23262..23342
                     /locus_tag="VVN16_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            23852..24040
                     /locus_tag="VVN16_000796"
                     /pseudo
     CDS             23852..24040
                     /locus_tag="VVN16_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            24455..25072
                     /locus_tag="VVN16_000797"
     CDS             24455..25072
                     /locus_tag="VVN16_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU5572220.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(25392..26279)
                     /locus_tag="VVN16_000798"
     CDS             complement(25392..26279)
                     /locus_tag="VVN16_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5572221.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVLQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(26428..28311)
                     /locus_tag="VVN16_000799"
     CDS             complement(26428..28311)
                     /locus_tag="VVN16_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU5572222.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSIPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
BASE COUNT         8421 a         6318 c         5365 g         8396 t
ORIGIN      
        1 gtagatttta gtttcaaaaa attcttttat ataataatgt ccttgaaagt agtttcagct
       61 gaacatttat agtccataaa aagaaaacag aaactcatct gtttttatac tcaatgaaaa
      121 tcaaagagca aactaagaag ctagccgcat gatgctcaaa acactgtttt gaggctgtag
      181 ataagactga caaagtcagc tcaaaacacc gttttgaggt ggcagataga actgacgaag
      241 tcagctcaaa acaccgtttt gaggtggcag atagaactga cgaagtcagc tcaaaacacc
      301 gttttgaggt ggcagataga actgacgaag tcagctcaaa acactgtttt gaggtggcag
      361 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
      421 tcagctcaaa acactgtttt gaggtggcag atagaactga cgaagtcagc tcaaaacact
      481 gttttgaggt ggcagataga actgacgaag tcagctcaaa acactgtttt gaggttgtgg
      541 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
      601 tcagtaacca tacctacggc aaggcgaagc tgacgtggtt taaagagatt tttgaaaagt
      661 attaacacct acagacaagc ctgacgatag caagaactac cctactcgat aggtatcggc
      721 ttttgctttc tgaaaaaaat tattttaagc aatttgacaa atctagcaac aaaaaattct
      781 ataaatataa tagattgaaa ctagaatagt acacattttc ttctaaaaca ttgttagaaa
      841 tcgatttgac tctcctgatc gttttgtcct attcttgttt cattttacta tatttccatg
      901 ataaaacgca tagtatcaag tttttttaat cccctgatac tatgcgtgtt tgtaattttt
      961 aagattttgt gcttagagtc gactccttat tttagatatt taaaaggaat ctcacttcca
     1021 cagagccagt tgtagacttg gtcattgaca aatacattca tggcttcgtg accatactca
     1081 ggcatgatgc gataggtttt atcgcaggtc agacgattat aaatcgcaaa ctgggtaatg
     1141 ggatagcaaa catcgtcgtc caagcccgta atcatcttaa cctcaccttg gatacgatgg
     1201 gcaagatttt tgacatcgat ataggcaagg gtcgccatga tttcctcctc tgtttcatgg
     1261 aaggggtcgt gaaacttgaa ataacggaaa agttcgtcgt aagcctcgct agtattacca
     1321 atctcaatca cccgtctgaa gtctgacaag aagggataaa tggcaactgt tttctgaatt
     1381 cgaggattga gcgctgctgc aactagagct agagcccctc ctttgtgagg caccatagct
     1441 agaaagacgc ttctcatcaa cctgagacag actagcaaca atttcgacca actggtaaat
     1501 atccagataa acatccttat aaaagaggtg gtcccgacct tccatagcac cacggataat
     1561 atgccccttc acggtatttc ctaaaggaga acgcaagccg tcttgtgagt aacctgactg
     1621 gccccgcaca tccatggaaa caacaccgta accagctacg gtgaagccca gcatgtcggc
     1681 ccagtcccag ccacgtccca tataaccatg aaaatggaag attagtggaa ccttctcctc
     1741 actctttgga agaacaatgc gtgcatagac ctttccttcc ttgcttcctt caaatgttaa
     1801 ctcatagcac ttgacttgag gaatgtggaa atctctttcc tccaagtggt aggatggaag
     1861 cgtggaaaca tttttcactt ccccatccca gaaagcatca aagtcttccg gaacctcatc
     1921 ccttcctcta taggtcttaa tttcttctag taaagctgga tttttcatag catgctcctt
     1981 ttattttgta atcgttacca caactgtagc atatctatgt aatcaatgca agaaagaagg
     2041 ataaatagaa agtgaattta gattctttac ttttaggatg atgggctgaa agtagtttta
     2101 gaaacgaaaa agagcatttc gctctctctt ctgtttatac tagttttttg cttccttctt
     2161 ttggaaagtg gtttggtaat ttactttaat cgttactgtc atatgagagt cctcgtttct
     2221 ttttgatgac tttagtataa gagccaaatc taaaaactag cttaagtttt ccttagagaa
     2281 agcttaatcc agatgttctt tcttttccag atgaagggca atcatgcgcc actctggatc
     2341 ttcttgccaa gcttctatag aactaatgta gctagcaacc tttcttgctt cttctaaaat
     2401 cggaaaatct tcgataatat cagccacttg gaactctgga agtcctgact gtctggttcc
     2461 aaaaatctca ccagaaccac gcattttcaa atcttcctcc gcaaggacaa atccattggt
     2521 cgtttctgtc atgatacgca tgcggtcttt cccagaatcc gtcttgggat tggcaacgag
     2581 aacagcgtag gactgcttgt ccccccgacc gacacgacct ctaagctggt gaagttgact
     2641 gagaccgaag cgatcggcat ccatgataat catgacagtc gcattgggaa cgttgacccc
     2701 aacctcaata accgtcgtcg aaaccagaat atccgtcttt ctctccttga aatcctgcat
     2761 gatctggtct ttttcgtcac tcttcatcct accatgtaga agagccacct ctgtcttgcc
     2821 tgcaaaatga gtcgtcaact cctctgataa ggcaatggca tttttcaaat ctagagcttc
     2881 tgattcttca atcaaaggag agatgacata gacttgggaa cctttttgaa tttccccctc
     2941 taaccaagtc aagacctgag gtagttgctc atgtttgatc cagcgcgtca caataggctt
     3001 ccgacctgct ggcatctggt cgataatgga aacatccata tctccaaagg ctgtgatggc
     3061 aagcgtccgt ggaatgggag tcgccgtcat catgaggaca tctggattgt cgcctttttc
     3121 ccgtaaaata cgcctttgcc ctacaccaaa acggtgctgc tcatcgataa taatcaaacc
     3181 aagacgagca tactccaccc catcttgtat cagagcgtga gttcctataa tcaaatcagc
     3241 ctcacccttg gcaatggtct ccaagacttc tctcttttct gcagctttca aggaacctgt
     3301 caagagagcc agtttcaaat tgggaaaaag gttctgtaaa ctctcaaagt gttgctctgc
     3361 gaggatttct gttggtacca ttagggcagc ctgataacct gctgtcactg ccgcaaacat
     3421 ggccaagcca gcgactaccg tttttccgct ccccacatct ccttgtagga gacgattcat
     3481 gtggtggtcg gacttcatat cagttaaaat ttcctgcaaa ctcttttcct gagcttgggt
     3541 cagggcaaaa ggaagacttg ctttaactgc tgtcactttt tcctgagacc aatccagaac
     3601 cagaccactt ccctgaactc tattttcaga cttgagcgtc tgcagctgca tttggaaata
     3661 aaagagttcc tcaaacttga tacggcgaag agcctgcttg tattctgcca aatcctttgg
     3721 aaaatgcata gctcggactg cctgacaacg ggacatgagt ttgtatttgt ctagtaaaga
     3781 ctggggcaga ttttcttcta tcaagaggtc cagtccctga tcaaaagccg tcttgatgac
     3841 cttgaccaga ctggcctgac tgattccctg agccagacga tagacaggct ggaggtcatc
     3901 ttctacctga gccagaacct tcatcccagt cagactagcc ttagcgcggt cccattttcc
     3961 aaagacagca agggttgctc ccaactctat tttatcagcc agatagggct ggttaaagaa
     4021 attcaccgca aaaacgacct ctccctgctt gagactaaaa cgcaggcgat tgcgcttgaa
     4081 accataatac tggacactag caggagtcac tacctgacca gaaagaactg ccttctcacc
     4141 gtcttccagc tccagcacct gcttggtttt gaagtcttca taacggaaag gaaagtagag
     4201 caagagatct tgcaagtttt caattcctag tttggcgtat ttttctgctg actttggtcc
     4261 cacaccaggc aagacatgca agggttgatg tagattcatg ctccactcct ttcttttcta
     4321 ataatattct ctcggaatac ggtcgctgag gaggcaaacc acctcatagt taatggttac
     4381 gcggtaggtc gctacctgag ttgcagtgac ttccttatcc ccattggagc caatcaaggt
     4441 taccttggtt cctagcggat aaagcttagg caatcgaata gtgatttggt ccatcgaaac
     4501 cctgccgaca attgggcaag cttggccatc taccaagaca gagaaatttt gcatgtctct
     4561 tgtccatcca tctgcatacc cgattggcac ggtcgcgatg acttgctcgc tatccgcttg
     4621 ataagttgct ccatagccca tgcaagctcc agctggaact gtcttgacat gaaccagagc
     4681 agactccaag gtcaaggccg gtatcaaatc ataaggcaaa tccaagaccg ctccacttgg
     4741 attgaggcca tacatggcat ctcccatacg aaccgcattg aaaatagtct ctacatgcca
     4801 aagagtcgtt gcagaattgc tagcatgaac cagctctgga acttccttca tactagctaa
     4861 aatagtttta aaccgttcta actgggcatt aaaatagtca tctgattcct catcagcagt
     4921 agcaaagtgg gtaaagattc cttcaacacg aacaccgtgt tgttgtagca aatcttgagc
     4981 ctgctcaacc tcactggcct ctctaaaacc aatccgtccc atccctgaat caatcttgag
     5041 gtggactgtc aatccagtta ggtccacttc cttatctaag agtgcttgaa tccactccag
     5101 tccagccact gtcaaggtga agtcatattc tttagctaga gcaacagctt cgatttcaga
     5161 aactcctaaa atgaggattg gcttgctgag tccagcttgt ctgagttcaa tggcttcatc
     5221 gatattggaa acgcaaaagc catcaacatc atcttgaatt gccttggcaa cggcaacagc
     5281 tccatggcca taagcattgg ccttgaccac agcccacttg agcgttcctt gagggatatg
     5341 agcccccatt tgctgaatat tttgtcgaat agctcccaga tgaatcagaa ccttggttgg
     5401 tctatgtgga ctagctttca tgattttcct ccaaaatgac actggctgtc acaaactgat
     5461 cggtgtggct gatagacagc caaatctttc ctgaaaatgg tgcctgacta aaataaggcg
     5521 ccccacgttc attgttcaag acttccaaat cctgaaaacc gagcttgcta atgcccgttc
     5581 ccatagcctt ggaaaaggcc tccttagccg accagcgacc agctaaatat tctatttgcc
     5641 tgcgtccttt gagactggtg aagcgctcca tttcctgagc ggtcagtaca cgcttagcaa
     5701 atccttcatg tcgtgtaact gcgctttcta tcgaagccaa ttcttcgatg tcaattccgt
     5761 gtccaactat cattctcatc aaaaggagtt gagattcccc aactccatcc ttttcactta
     5821 ttttgtcaag gtaacataaa tctcttctac caaggcctct gtattttccc aacctaggca
     5881 agggtcagta atagagcaac caaagatctc tggttggttt tgacgaccat ctgctaggta
     5941 agattcaatc ataaatcctc gaaccgtctt tttaattttc tcattccaat cacgattctg
     6001 caaggtctgg cgaacaattc gaatctgctc catatattgc ttgcctgagt tatcatggtt
     6061 ggtgtcaatg aggataaaag gattttcaag tcccatggtt tcatagcgtt caatggcttg
     6121 gagtaggttt tcatagtagt aattcggcat ataattgcca tactcgttga ctgctccacg
     6181 gaggataaca tgggccaaag gattacctga tgtctcaact tcctgcccat gataaaggaa
     6241 ggtttgcttg ttttgagcag catagatggc gttaaacata acacccaaat ttcctgaggt
     6301 tggatttttc atccctactg gtgcatcaat cccagaagcc acaaagcggt gctcttggtc
     6361 ttccacagaa cgagctccaa cggcatggta gctgaccaag tcatccacca agatcagatt
     6421 tgacggataa agcatctcat ctgccgttgt caaaccagtc tctgtaatca cgcggtagtg
     6481 caactggcgc acagcctgca agccattaat caggcttgga gccttagaag tatctggctg
     6541 gtgaactaat cctttatagc cgtctccatt ggtacgaggc ttagcagtat acacgcgcat
     6601 gaccatgaaa atcttgtccg ctaccttctt ttgcaaggcg gataaacggc gagcatattc
     6661 caagaccgcc tcttcattat cagaagagca aggaccaatc accaagagaa tacggtcatc
     6721 ttcccctgaa ataatatctg ccaattccct gtcacggctt tccttgagtc tcaaggcttc
     6781 cgcagacaat tgggtttcag ccttaatgac ttccatatcg atttcttgac ctttttcaat
     6841 aaatgccatc ttattctcct aacgttttgt aaatttctct gacaagagct tctgtgttat
     6901 cccaacccag gcaagggtct gtgatagact taccaaatac ttctggctca ttttgtcgac
     6961 catcttccag ataagactca atcataaaac cacgaacgaa ctgcttaatt ttttcattcc
     7021 aagcacggtt aatcaaggtc tggcggacaa ttcggatctg ttcaatatac tgcttaccag
     7081 aattgtcatg attggtatca atgatgataa aaggattttc caagcccatt ttctcatact
     7141 gggcaatggt atcaattaaa ttgtcataat agtagttggg aatatttttt ccatactcat
     7201 taagagcacc acgaagaata gcgtgtgaaa gcgggttccc agttgtttct acttcttttc
     7261 ctaagaaaag gaaactttgt ttgttttgag cagcataaat cccattaaac atgacattga
     7321 gatttccaga ggttggattt ttaaaaccag tagaaaatcc tgccccactt gccacaaagc
     7381 ggtgttgctg gtcttcaact gaacgggcac caactgccat gtaagaaatc aaatcatcta
     7441 caagcggaag gttttcagga taaagcattt catcagcagt tgtcatccct gtttctgtga
     7501 tgacacgata gtgaagatgg cgaacggctt tgattccatt gataagacta ggcgcttctg
     7561 tcgcgttagg ctggtgaatc aagcccttat agccatctcc gttggtacgg ggtttggcag
     7621 tataaacacg cataaccata aagatacgat ctgccacttc ttcttgtagg actgccaaac
     7681 gcttagcgta ttcaaggaca gcttcttcgt tgtcagatga gcatggcccg attaccaaga
     7741 gaattcgctg gtcttctcca cgtataatgg cttctagctc ttgatctctc tgtgatttcc
     7801 tctccaaagc ctgaccttct aattttgaca aggcacgaac ttcttctata tttattttag
     7861 agctttttgc tgtaaatacc ataactcatc tccttataaa gcaaacggat acccagtaaa
     7921 aatttacttt tcacaagata tccgcctcta aactatctca ttttattgtc ttttaccgtg
     7981 acagttttta aatttcttac cagaaccaca tgggcaaagt tcattgcgtc caatctggct
     8041 caaatccaaa tcttctggca tacttgcttg gtgagcagcg atattgcgag tcgctgttgt
     8101 actgatatga cgttctgcct gtggtctttc ttgttcatga atttgtgctt tcatcatcaa
     8161 gcgtgtcaca tcaaactcaa tcgaaccaat catatcatta aacatacgga aaccttctgc
     8221 ctgatactca acaacagggt tgttctgagc atagccacga agtccaaccg cgttacgcaa
     8281 ttgatcaagg gcatcgatat gatctgtcca cttgttatcc accactcgta gaatcaaaac
     8341 tttttggaat tctttaactg cttcttcatc gcgtagtttt gaaacctgac tatcgtaaac
     8401 cttcaaggca cgttggaaaa gctcttcctt gatggcctta tcagacaagc ctgacaagtc
     8461 ttccatcgta atagaatctt caggaagcaa gttgtactta gcaaagttca aaattgcctc
     8521 tagtttttca tcttgtttgg cacgcgcatg accatcaacg acacgttcaa tcgtgcgttt
     8581 gatcatagac tgaatttcag gtgccaagtc acgatctgca gtgatgacat cgtaacgttg
     8641 agcatagata atctcacgtt gttcacgcat gacatcatcg tattgaagga cttgtttacg
     8701 ggtatcgtag ttatttcctt cgacacgttt ctgagctgct tcaacctgac gcgtcaacat
     8761 gcgagactca atcgcctctt cagacatgtt caagcgttca aagattccct tcaagcgttc
     8821 agaaccaaaa cgtttcatca aatcatcttc aagagatagg tagaattgtg actcacctgg
     8881 atctccttga cgacctgaac gtccacgaag ctggttatcg atacgacgac tttcatgacg
     8941 ttctgtacca ataacacaaa gtcctccaag ttcacgaaca ccttcaccaa gcttgatgtc
     9001 ggtaccacga cccgccatgt tggttgcgat ggtaacggca ccacgttgac cagcattcat
     9061 gatgatttgg gcttctctat agtggttttt ggcattcaag acttcgtgag gaacaccagc
     9121 tgcaaccaat ttcttagaaa tgtagtcact agtttcaacc gctactgtac caaccaagac
     9181 aggttgaccc ttttggtaac gagccttaac gtcttcgaca accgctttaa acttagattc
     9241 gatacttgca taaagaaggt ctgagtggtc aatacgttga acaggacggt ttgttgggat
     9301 tggaataaca cgaatgttgt agatttcacg aaattcttct tcctcagtct tacctgtacc
     9361 cgtcatacca gacaattttt tgtacatacg gaaaaggttt tggtacgtga ttgaggcaga
     9421 tgtcttggtt tcatcctgga ttggcacacc ttctttggct tcaatagctt ggtgcaatcc
     9481 atcagaataa cgacgacctt ccatggtacg acctgtaaat tggtcgacaa tcaagatttc
     9541 ttgctcttcg ctcaccacat agtcaatatc gagaagcatg atgtagttgg cacgaagggc
     9601 gttatcgata aagtgagtca aagccacgtt ttcgatgtca tagaggtttt caagtttgaa
     9661 gtagctttca gccctgtcaa tccctgaatc agacaaacca atagtcttag actgcacatc
     9721 gatgatgtag tcatctttgt tcaaagattt tacatagtgg tctgccatgt gatacaactg
     9781 actggtttca accgcattgg cacctgatac aatcaaaggt gtacgagcct cgtcaatcaa
     9841 gatagagtca acctcatcga ccaaggcata gttaagcgga cgttgtacca tgttttcggc
     9901 gcgaacgacc atgttgtcac gaaggtagtc aaatccgatt tctgagttag ttgagtaagt
     9961 aatatcacac tcataggctt cttttttctc cattggagat ttggtagcca agttaatccc
    10021 tactgacaaa ccaagccaag agtacaattc acccatctca gtcgcgtcac gttctgacag
    10081 gtattcatta accgtaacta cgtgaacccc tttacctgag agggcattga ggtataccgg
    10141 catggtcgca gtcaaggttt tcccttcccc tgtacgcatc tctggcacgt caccatggtg
    10201 aagaacaatc ccccccatga cctgaacctt ataagggaag agacctagga cacgtttggc
    10261 accttcacgg acaaccgcaa atgcttcgta aagcaatgaa tccagtgatt ctccattttg
    10321 ataacgttcc ttaaattcaa ctgtttttgc ttttagttgg tcgtcagtca aagcagccat
    10381 ttggtcttcg tatttgaaaa ccttgtcagc catcttttcc agacgacgga tttctccttt
    10441 atcattttcg ataattgttt ttaaaatatt agccatgttt ttccttactt taaattccga
    10501 atattttaga atgttctttt aattttagca caattactga ttattttcaa ggaagaatcc
    10561 ccattttgaa aaggaaaaag aggctagttt ccaagctaac ctctctgact tttatgatgc
    10621 tttaattgcc aaaaatcggc aaaagagcaa ataggataaa tagatttatc caaagagaaa
    10681 gacagcaaat aagtccaaaa acgaagagac tgactttctt ggataagaag gccatcaaaa
    10741 tggacagaat gccaaaaacc agcaagattt tctgtatgat gaagatgtca atccttaccc
    10801 ccagaatcgc tctatcccaa gggataaaga agaaagacag ccagatcagc agaactaaac
    10861 caatataaac cttggaatag cgtgtaataa atgattttag atgtgccata agccatctcc
    10921 taggattgta tatcctttac atttgtatat gtccattttc gacaactaat acatctgtac
    10981 aaaggtctga aatatcttcc gatatgtgcg acgtcaggat aaccagacca ttttcttttt
    11041 tcaggtaaga taaaatgacc tgtttggtta acctcacact cttctcatcc aaagcattca
    11101 taggttcatc gagaaagagt atagaaggag aggaaataaa ggcttgaatc aaggccattt
    11161 tttgctttgt tcccaaggat aaatgccggt attcaacgtc ttcaaattcc tgtaaatcat
    11221 agtattgaat ccaataggca attctttttt ccgtaacttt agatgaaaaa taccttaaca
    11281 gttccaaatt ttctctcagg gataaatgag ataaaaactc gactttttca attaaaattc
    11341 ctgcatcctg aatataatga ttttttatcc cgtaaacttt accatcttga agaacttttc
    11401 ctttgtcaag cttaatataa ccagcaagta cctttaaaag aaccgtcttg ccagatccat
    11461 tatcaccttt aagtccataa atttttcctg attgaaacac caaattcaac tgatttaaaa
    11521 taggtctttt cccataaatt ttctgtccat ttataatttc aagtttcatt gtttatcctt
    11581 tcttaaattt ctcttccaag cgactaacaa tcaacataac taataagctt aagagtagta
    11641 aatacactgt tacaatatga tccatcatcc attcttgtcc cacaaatgct aaaaaataga
    11701 gaaaaaagga aaaaccagcg cccgcactaa tcagtgctat cagataagac agtagtaagt
    11761 acaacatcat taactgagca agtaaaaaga aaagtgcgac tgttttgaaa tgaattccta
    11821 gcggaagtaa accaaggaac cagacaagta aatcttgagt aaaaaattta gaaatgacaa
    11881 ccaatcgttt ccttgtataa tcgaaaaagg agcttgattg atagcgaatc aagtgatggt
    11941 aatggtctgc caaatcactt tcaagtaaaa tgacagagag ccatcctacc atgaaaagcg
    12001 aaaggtttaa caagagtttg gggatttcta aattcgcttc agaatagtca ttataggaaa
    12061 agattgaaat caattctctc ttgaaaaaga taacagcatc ctgcttttga accgctgttg
    12121 acaatagata gtttatcaaa atcatcatca gaactatacg aagtagtaaa acgatctttc
    12181 tctttagcca aatattgtta aaattcttaa acattatctt catatttttc aagcaccata
    12241 taagggacaa tataaaggat ataaggagta aagagtttca tcaaacttac atccccatag
    12301 ctagcttgca tcaaactcgt cataggaacc atatagtaag gcaaggcact atagagcagc
    12361 atagaaccaa gccaaagaaa catcaaatag gtgattgccg aacgagtcac attccccaca
    12421 taatcaacta tttgtaaaaa atagattgca ttgatgaaaa taccgattag taggacacaa
    12481 gtaaagaaca aatagctttt tatctctcca tctaggagtc tttgtaaacc acttccatca
    12541 gaaaatagag aattccatcc aagaggagaa aaagtcccaa agaaataggt tataattgca
    12601 attatcagca cagtcactaa atatatcaaa caagggatac ttgcaacttg caaagtcaac
    12661 tttcttttta acccttgata actcacttct cttccaatac tcaatcgtaa aactttattt
    12721 ttcagctcaa tatattgaaa acctagcaaa acaatgatga ttggaataat aaatgattga
    12781 taaaaaccta gattgaactg gagaatacga aaatctaatg ctgattttaa taagtcattt
    12841 ttaattgctt gtccatcgaa tttagctaga tcgggcgcat gaatatcaaa aaagaaataa
    12901 gtcatcaaaa catcacgata aaccaaagca agaaaaagca aagtaagcaa gacataaaca
    12961 aatttatgcc ttgcaaaagt taagagagga atcttcataa gccacctcct acaaatagaa
    13021 agtaatataa tactcaatga aaatcaaaga gcaaactagg aaactagccg caagttgctc
    13081 aaagcactgc tttgaggttg tagataagac tgacgaagtc agttacatat ataatccaag
    13141 gcgacgttga cgtggtttga agagattttc gaagagtata aatcaatgcc ttccgccttt
    13201 agacttccct agttcccgtc tcaagtcaag catttttttg aaacagatat agtctaacca
    13261 gtcactagca aaagcgataa gtagtaataa tagccaaata ccccataact tgatatctgt
    13321 caaatttctc aagacgatat tgaaaaacag aactgaaaaa actgtccaag caaggctaaa
    13381 aagagaatag agggtgatgt aaatccagta aaaatagtaa aaaattggga aaaatttact
    13441 ttctctatta tccttgtcaa tccagctgaa aaagtaaaaa cagggtaata agattatcat
    13501 tttaaacaag tgttccatca caagcctcct tttttaaatt gttttcttta gtttcgttct
    13561 attaaatgtt attaccaata taaaccttgg aatagcgtgt aataaatgat tttagaggta
    13621 ccataagcca cctcctacaa atagaaaccg atataaatca atgccttcca cccttagact
    13681 tccctagttc ctgtctcaag cgaaacattt ctttgaaaca ggaataagtt aaccaattca
    13741 taccaatagc tagcagaata aaaagaaacc aaatgcccca taacttgata tctgtcacat
    13801 ttctcaagac ggtattgaaa aacagaactg aaacaactgt ccaagcaagg ctaaaaagag
    13861 aatagaaggg gatgtaaaac cagtaaaaat aataaaaaat tggaaaaaac ttactatttc
    13921 tgttggcctt ttcaatccag ttatcaaaat aaaagtacgg tgctaaaagt aagaatttaa
    13981 acaaatgttc catcaccgac atcccccttc ttttgatagc gttttctatt attttattat
    14041 atcaaaaaaa tccggaactg tcattccaga ttctactttt ttatttgcgt tttcttgcga
    14101 tgagatgaat cggtgttccc tcaaaaacaa aggccttgcg gatttgattt tccaagaaac
    14161 gcaggtaaga aaagtgcatg agttcttctt cattgacaaa gatgataaag gttggtggtt
    14221 tggttgccac ttgggtcgca tagaaaatct tgagacgttt tcctttgtct gtcggtgttg
    14281 ggttgatggc aatggcatcc atgatgacat cgttcaagac agctgatgga atacgtgtat
    14341 tttgactttc gctgatttgc ttaatcatct caggaagttt gtggagacgt tgcttggtta
    14401 aagctgatac aaagataatc ggtgcgtaag gcaggtattg gaactgctca cggatatctt
    14461 cttcccagtt tttcatagtg tggttatctt tttcaagcgt atcccacttg ttgaccacga
    14521 taatcatccc tttaccagct tcatgggcaa atcctgcgat acgcttgtcg tactcacgaa
    14581 tgccttcttc cgcattgatg accatcaaga ccacatctga acggtcaata gcacgcatgg
    14641 cacgcataac agagtatttc tcagtatttt cataaacctt accagactta cgcataccag
    14701 ccgtatcaat catggtaaac tcttgaccat ctgtatctgt aaagtgggta tcaatggcat
    14761 cacgagttgt tccagcaaca ggactagcaa taacacggtc ttctcccaag atagcattga
    14821 tcaagcttga ttttccaacg ttaggacgac caatcaagct aaacttaatg acatctggat
    14881 tttcttcctc atattcattt ggaagatttt ctacgatcgc atctagcaca tcccctgtac
    14941 cgattccatg gacagatgag ataggcaatg gttcacccaa accgagagca tagaaatcat
    15001 agatatcatt tctcatctca gggttgtcca ccttgttgac tgcgaggata actggtttgt
    15061 gggtcttata aagcttacga gctacgtatt cgtctgcatc agtaattcct tccttaccag
    15121 acacgacaaa aacgataaca tctgcttctt ccatggcaat ttctgcctgg tgcttgattt
    15181 gttccatgaa aggagcatcg acatcatcaa ttcctcctgt atcaatcatg ctaaaagaac
    15241 gattgagcca ctcacccgtt gcataaatac ggtcacgtgt cactccttcg acatcttcta
    15301 caatggagat tcgctcacca gcgatccgat taaatagggt tgatttccca acattgggac
    15361 gtcctacaat ggcaatagtt ggtagggcca taatttctca ctttctacaa taatttcttc
    15421 tgttcaagat tttttctagt tgagcttggt tcagcttgac caaactgttc tgctaggcgc
    15481 tgactccagc ttgtggtcgc acgcgcccca gcatagtcag cctgaacacg gtcataagct
    15541 tggattgcct cagttgactg ttcttggtat tcttcctcaa agacaacatt ctctagtggc
    15601 agtctcggtt tcatatcatg atgttgattt ggcacaccca gtgccatccc aaagacagaa
    15661 taggtgtagt caggtaggtt aaagagctct gccacttctt cagacttgta tcgaaccaaa
    15721 ccgataatca caccaccata gcccaagctt tcagctgcca acaaggcgtt ttgtccagca
    15781 agagctgcat cgaccgaact aatcaagaga ccttccacac cttggggttg gaaggtgtcg
    15841 gtatgaagtc gggctccctt ttctgctcgg ttcaaatctc cgacaaagag aaggaaaaca
    15901 gcagactggc gaatggcttc ttgaggtacc aattcataca aggcatcttt cttctcttgg
    15961 cttcgtacca caatcacaga gtaggattgg aaattcttcc aagatgatgc catctgggct
    16021 gctgtcagga tttcagtcaa atcttcctga ggaaggactt gctccttaaa tctgcgcact
    16081 gaagtatgag ccttcatcaa tttaatgatt tctgtcatcg acggtttact ccttctaaac
    16141 gagtctcctc agccaaataa cggatgcgtt ccatgacccg tctggcttcc caggtttcgt
    16201 catttccatt tttcccttta gcgaaatgct tctccaaatc ttcaaagttg aagttagagg
    16261 tgaaaaaggt cggtaaattt tcctgcatcc gatattggag aatgacctgc aggatttcat
    16321 cacgcaccca agcggtggat tgctcggcac caatatcatc taaaatcagg acttcagaaa
    16381 gcttaatctc atccactaag gtcttaacat tgccatcacc gatagcattt ttgacatcaa
    16441 tgacaaagct aggatagtgg aggagagtgg aggatacacc acgtttttct gataaatcat
    16501 gagctagggc agccaccatg aaacttttac ccacaccaaa gtctccatat aagtaaagac
    16561 cttttcgaat agctggatat tgctccacga aggctagtag cttttcaaaa actggtaagc
    16621 gccccaaatc atccaagtca acttgagcca aactagcttt cttgagactg gctggtagat
    16681 tgattaactt gagacggttc ttaatagccg cttctttttc agtcgcgatt agctcaggag
    16741 tttcttcata tgaaacatct gcatagccat gattcttaac caaaatcggc ttgtagcctt
    16801 tggcaatata atccgtatcc ccacggagaa acttgtcacg ctcggtgatg tactgattaa
    16861 acttggagat actgcgattt aattcctctg gagtcaagga ttcttgctgg ataaaagccg
    16921 caacatcagg gtccttcatg attttctgga ccaaatcttg ataataaaaa cggctgggtt
    16981 gacgtttgag tacgtctccg acactttcca tctaatctcc tcctttttct aatcgagcta
    17041 atagttcttg cttcttacgt tctagttcca gacgagtttc ctcgctggtt tcattcttat
    17101 attcaggatt actccattta ggaacattgg tttttactgg ggcagtctga ttctgttttt
    17161 gtgtttttgc tttctgtcct cgctcacgga tgcgcaagac tgccttttct gccgaatgaa
    17221 tcttttgata ggcatagtca ttggctacct tcatggcata tttctcattg atatttgccg
    17281 aatcgacctt attaaaggtc aataaaagaa tgatattgat gacttcgtcc agcaagccca
    17341 agccagccat ctgttgcaag agttctcttt ctgtttgggt aatggttccc ttgcgtgttt
    17401 gcttgatttc tgccaagaac tgcagggcag ttttactttt ggcttctttg ataatggttg
    17461 tttccttaag actaaagtca gaggaaactg gtttttgagc aattttttca cgcatgcgtt
    17521 tagttgaaat aacctgagaa acagctgttg acttggccaa ttgataggtt tcaaaccaag
    17581 tccatttctt ctcctcggca atagcaaaga ggtttaagac atcggactgc tcatccgcaa
    17641 aacgaagtcc atctcgagcc atcagctggc gaaaatgttc caagtcaaaa tcattggcca
    17701 ctttcttctt gagaccaagg tcttcttgac tgcctagttc tgccaattct ggaaagactt
    17761 gattgagtga gacaggtatt tcttcaccat cagcactttc aactttcaaa tcctccacag
    17821 cagagtcgcc aatctttttc tctaagagtc tgcgataaac agtatgctcc aagaagtctt
    17881 gactagatag aggagcatgg agggctagct gataaacatc ccccttttga tagagggtca
    17941 agagattaaa agcagataag attttcaatg attttatcag tctatccatc ccaaagttga
    18001 gatgattgag gatacttgaa aaaagatatt cctttctacc attatcccaa aaactgattg
    18061 tataaagata aaggctcagt gcctcctgac cgataatcgg gaggtagcac tgtaccagag
    18121 atgaggtatc ttgcgacacc cgattattct ttagataaga aaaacggtca attggcttca
    18181 tttatctttc ctttttcttt ttagaggact gggtgatttg ttggagcaag ctctctaact
    18241 cactgacatc cttaaaacta cgatagacac tagcaaaacg tacataggta atctcgtcca
    18301 attcagccaa ctcctccatg acgagtgaac caatgtcctc actttgaatt tcattttcat
    18361 ttcgaccacg gagtttctgt tcgatacgat tgactaccat gttgatttca tcacttgaca
    18421 caggacgttt ctgggctgag cggataatcc cattaaagat tttatctctg gagaattgtt
    18481 cccgtgtgcc atctttttta acaaccacta aggttctttc ttctactcgt tcgtaggttg
    18541 taaaacggtg ttggcattcg tcgcactcac gtcttctacg aatggtgttc ccttcttctg
    18601 cttggcgact atcgataaca cttgacttgg tagccccaca ttttggacaa cgcatccttt
    18661 ccctccttat cgttttcttt tcattatacc attttttaag cgattcccaa aacaattctt
    18721 ctttttgctt gacaagtttt ttgttttgtt gtattattta attaagacaa caaggtaaaa
    18781 gaaaggagac taagatgtcc tggacatttg acaacaaaaa acccatctat ttacagatta
    18841 tggagaaaat caagcttcag attgtttccc atacactgga acccaatcaa caacttccaa
    18901 ccgtgaggga gctagctagc gaggctggtg tcaatcccaa taccatccaa agagccttat
    18961 cagaccttga acgagaagga tttgtctaca gcaagcgaac aactggacga tttgtgacta
    19021 aggataagga gctaatcgcc cagtcacgca aacaattatc agaagaagaa ttggaacact
    19081 tcgtttcctc catgacccat tttggctatg aaaaagaaga actaccaggc gtagtcagtg
    19141 attatattaa aggagtttaa gcctatgtca ttactagcat ttgaaaatgt atccaaatca
    19201 tatggagcaa caccagccct tgaaaatgtt tctcttgaca ttccagctgg aaaaattgtc
    19261 ggccttcttg ggccaaacgg ctcaggaaaa acaaccctga ttaaactaat taatggcctc
    19321 ttacaaccag atcaaggacg tgtcctcatc aacgacatgg acccaagccc agcaaccaag
    19381 gccgttgtag cttatttgcc tgatacgacc tatctcaatg agcaaatgaa ggtcaaagaa
    19441 gccctaacct acttcaagac cttctataaa gatttcaatc ttgaacgcgc tcatcatcta
    19501 cttgcagacc tgggcattga tgaaaatagt cgtctcaaga aactatcaaa aggaaacaaa
    19561 gaaaaggttc aactgatttt ggttatgagc cgtgatgctc gtctctatgt tttggatgaa
    19621 cccattggtg gggtggatcc agcagcccgt gcttatatcc tcaataccat tatcaacaac
    19681 tactcaccaa cttctaccgt tttgatttct acccacttga tttctgatat cgagccaatc
    19741 ttggatgaaa ttgtcttcct aaaagacgga aaagtcgtcc gtcaaggaaa tgtagatgat
    19801 attcgctacg agtcaggtga atccattgac caactcttcc gtcaggaatt taaggcctaa
    19861 gcaaaggaga ttatttatgt tttggaattt agttcactac gaatttaaaa atgttaacaa
    19921 gtggtattta gccctctacg cagccgtgct agtcctttct gccctcatcg gaatacagac
    19981 acaaggcttt aaaaatctac cttaccaaga aagtcaggct actatgctac tttttctagc
    20041 tacagtcttt ggtggcttga tgcttacact tgcgatttca accattttct tgattattaa
    20101 acgcttcaaa ggtagtgtct acgaccgaca aggctatctg actttgacct tgccagtttc
    20161 tgaacaccat atcatcacag ccaaactaat cggtgccttt atctggtcat tgattagcac
    20221 cgctgtattg gctctaagtg ctgttattat tctggcttta acagctccag aatggattcc
    20281 tctttcttat gtgattacat ttgtagaaac acatctccct cagatctttc ttacaggtat
    20341 atccttccta ctaaatacca tttcaggaat cctctgcatc tacctggcta tttccattgg
    20401 acagcttttc aatgaatacc gtacagcact cgctgttgca gtctacattg gtatccaaat
    20461 cgtcattgga tttattgaac ttttcttcaa tcttagttct aatttctatg tcaattcact
    20521 ggtaggactc aatgaccatt tctatatggg agcaggtata gccattgttg aagaactcat
    20581 attcatagct atcttttatc tcggaaccta ctacatcttg agaaataagg ttaatttgct
    20641 ttaaataatt tttacctaga tatgtaacat actcatagaa caaaagagac caggcaaaaa
    20701 gtctttaaaa ttagaaaacg catagtatca agtgttgaat atgaactgcc cccaaaagtt
    20761 agacagaaaa aatctaactt ttggggggca gttcaaaaaa tccttgatat tatgcgtttt
    20821 ttgtgagctg acttatttcc tttcactata tcgcaaaatg aaataagaac ggaacgatgg
    20881 gattttggaa ttcaaatcaa tttgtaagaa tgttttagaa gtaatattat cctattccag
    20941 attcagttca ctatacaatt gagttttcaa gcaacttgtt tacataatgt gtacataatt
    21001 aggttcgtga ttccaccctt ttcaccttta aaaacctcgc tttcgcaagg ctcttctatt
    21061 tataagataa ggcacgttta aaggttttcc aaatccctaa atcatccgtt tgaagaacga
    21121 gactagcata catgcgtccg ataaatcctg ttgctaccac cgcaaaaatc actgtaatag
    21181 caagtgaaat ccatgcttct gctccccccg catagtcatt aatcgttcga aacggcataa
    21241 agaaggtcga aataaaggga atataagaac caatcttcaa gaggagattg tcaccagctg
    21301 cacctagagc tgtcactcca aaaaaaccac ccataatcaa aatcatcaaa ggcgacaagg
    21361 ctttccctga gtcctcagga cgagaaacca tagatcctag gaaggctgcc aagactacgt
    21421 acatgaaaag actgatcaaa ataaagagca aggtattcag tgagatagca tctcccaagt
    21481 gaaccaaaat accagactga gccaagaatg gcaaatcttt aaagagcaaa acggcagcca
    21541 gaccacctac aacatagatc ccaatatgcg ttaaaatcac tagaaacaga gccatcatcc
    21601 gcgcatagaa atagtgactt gcccttatgc tagaaaaaac gacttccata attttggtgc
    21661 ctttttcact ggcaacttcc tgagctgtta cacccgcata ggtaatcaga atcatataaa
    21721 gaaagaatcc taaggcacct gctgcaattg tttgaataaa ctttttattt tccttggctt
    21781 catcaatctt ttctgtgaat tgaattgtct gcgctaagcg tttttcctgc tcttgagaca
    21841 aggaagcagt tgaacgatta agctgatttt gcagttcatt gagtgtacct gtaacctcaa
    21901 atttaattcc attttcaagc gatgtttcgc catgataaac tgcctttaga acactatctt
    21961 cttgatcaat ggtcaaataa ccttttaatt tttcttcttt aattgcttct ttggcacttg
    22021 cttcgtcttt atagtcgaag ttaacaccat ttacattctt cagtccttct gctacagatg
    22081 gcactgttgt cactactgcc actttattat ttttagccat agaagaacct tggatatgcc
    22141 caattcctac agagattcct aaaaagagga acggcgaaat caccataaag aagaaactcc
    22201 atgacttgac atgtcgaaga taggtttcct tgattacaac ccacatattt ctcatacttc
    22261 cactcctgat tctagtttaa agatttcatc gatagttggc gcttgttggt caaatgttgc
    22321 gatatattga ccttgagtca agattgggaa gagttccctt ccagcgctct catcctccaa
    22381 aatcaatttc caactgcctt gtttggtcaa gctcacctgt ttgacatgag gaagattttc
    22441 caattcttcc ttgcttcgtt cacttgaaac aaagagacgc gttttcccgt attgattgcg
    22501 gacatcctga actggtccgt gcaagaccac acggccatct cggatcatca gaatatcgtc
    22561 acaaagttcc tcaacattgg tcatgacatg gtcagaaaag ataatggttg ctccgcgctc
    22621 tttttcctga aaaatgactt gtttgagcaa ttctgtatta actgggtcca atccactaaa
    22681 aggctcatcc aagataatca ggtctggttc atgaatcaga gtaataatga gctgaatctt
    22741 ctgctgattt ccttttgaca gactcttgat tttatctgtc agctttcctt tcacttccaa
    22801 cctcttcatc cattgaggga gtttttcttt gacttctttg gcatccatgc cttttagagt
    22861 cgccaagtag cgaacttgtt caagaactgt caatttaggc atgagactgc gttcttcagg
    22921 cagataacca atccgagcat aggtctcctg acgaatatcc tgaccatcca gaccgatttc
    22981 tccctgatat tctaagaatt tcaaaatact atggaaaatc gttgtttttc cagcaccatt
    23041 ttttccgact agtcccaaaa tacgacctgg tcgcgcttga aagtcaatac caaacaaaac
    23101 ttgcttggat ccaaaacttt tctctagact tcttacttct agcatctttc acctccgaaa
    23161 tttcttgcac tcattatact cctttttgat agcctttaca atgttttttg tccattttta
    23221 gaagactatt gctgtgtaaa atatggcctg gagcactttt atactcaatg aaaatcaaag
    23281 agcaaactag gaagctagcc gtagactgct caaagtacag ctttgaggtt gcagataaaa
    23341 ctgacgaagt cagctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta
    23401 actatatcta cggcaaggcg aagctgacgt ggtttgaaga gattttcgaa gagtattagt
    23461 gataaatcca ttatacagca gcaaacttaa tttatacctt ccgctcctca actgtctatt
    23521 tttaatcctg aattgttatt tgagtaactc ctttttcctc gtaaagtttt cttcctctaa
    23581 aacttctgga aaaaggctaa tagtttcaga caacattttt ataagaaaca agttcatctg
    23641 tcatttcaag aaggagtaat cctttatcta ctaatggacg gaacagaatt caaccgcttg
    23701 tccgatatgt tttctaagga ttatatagta aaatgaaata agaacaggac aaattgatca
    23761 ggacagtcaa attgatttct aacaatgttt tagaagtaga tgtatactat tctagtttca
    23821 atctgctata tctattatgc acacccctat aggatctaat gaaaatcaca acaggctcat
    23881 tcatagatgg ttacctcagg aaactaagaa aacgactacc aaggaagtcg cattcatcga
    23941 aaagtggatt aacaactatc ctaaaaaatg cttgaactac aagtccccca gagaagactt
    24001 ctggatgact aacttgaact tgaaatttag caataattaa ttcaccatct aactatattt
    24061 agtaattatt tcagaactga ttaatattaa aattaactaa caattcaaag gattcatact
    24121 agccataaat tacgtccatc agagagagac tcttactact tttagatttt agtctttcta
    24181 gcttcagaat acatctaaac tttagagaaa atgactattc gaaagcgcga atgcctcaaa
    24241 attatctcag ataagctatt cgaaacttag aatgctttta aatttatgga attgcgatta
    24301 ttcgaaacct agaatgcata taacctttag ttgacagacc tattctaagt ctcgaagggc
    24361 tatttacttt ctattcctta tcaaaaaaga ctcattcccc ctttctcctc caaaatatgg
    24421 tatagtagaa atatactatc tatgaggagt ttacatgtca caggataaac aaatgaaagc
    24481 tgtttctccc cttctgcagc gagttatcaa tatctcatcg attgtcggtg gggttgggag
    24541 tttgattttc tgtatttggg cttatcaggc tgggatttta caatccaagg aaaccctctc
    24601 tgcctttatc cagcaggcag gcatctgggg tccacctctc tttatctttt tacagatttt
    24661 acagactgtc gtccctatca ttccaggggc cttgacctcg gtggctgggg tctttatcta
    24721 cgggcacatc atcgggacta tctacaacta tatcggcatc gtgattggct gtgccattat
    24781 cttttatctg gtgcgcctat acggagctgc ctttgtccag tctgtcgtca gcaagcgcac
    24841 ctacgacaag tacatcggct ggctagataa gggcaatcgt tttgaccgct tctttatttt
    24901 tatgatgatt tggcccatta gcccagctga ctttctctgt atgctggctg ccctgaccaa
    24961 gatgagtttc aagcgctaca tgactatcat cattctgacc aaacccttta ccctcgtggt
    25021 ttatacctac ggtctgacct atattattga ctttttctgg caaatgcttt gacacgtaaa
    25081 aaatccgttt ggtttcccaa gtggattttt aaagcgtaga ttaactatag cttgatacta
    25141 aatatacttt ggtatggaaa tcatgcatat ttttcgatag tgaggcgagg acttacctag
    25201 ccttcccgcc gtgatagaaa cacctgaaat ctaatggttt caggtattcg gaaactttga
    25261 gcctagtgtc tcaaagttta ggtatggaat ttcgaagaaa gtcgctaccg tccgtaatca
    25321 cttaaggaaa ggctcaaaaa tattgttttc aaccacaaaa tccgtttggt ttcccaagcg
    25381 gattttgtgc tttattttga aacttctttt gcaagaacaa agttcccaag tgtggcagaa
    25441 ccatttcctg cgactgctgg cgtcacgata tagtcacgca catctggtac tggtaggtaa
    25501 ccattaagaa gagatgtaaa tttctcacgg acacggtcca gcatatgttg ttgagccatg
    25561 acccctccac caaagacaat cacgtctggg cggaaagtca ctgtcgcatt aaccgcagct
    25621 tgagcgatat agtaggcttg aacatcccaa acagggttgt tgagttcaat attttcccca
    25681 cgtacacctg tacgagcttc caaacttgga ccagctgcat aaccttctag acatccctta
    25741 tggaaaggac aaacaccctt aaactctttt tcaatatcca ttgggtgtct agcaacataa
    25801 taatgaccca tttcagggtg acccacacca ccgataaact caccacgttg gaggacacct
    25861 gcaccgatac ctgtaccgat tgtgtagtaa accaagtttt cgatacgacc accagcattg
    25921 ttacgggcaa ccacttcacc gtaagcagag ctgtttacgt ctgttgtgaa gtacattggc
    25981 acgtttaggg cgcgacgaag ggcaccaagc aagtccacat ttgcccagtt tggttttgga
    26041 gtcgtcgtga taaagccata agtttttgag tttttgtcaa tatcaatcgg cccaaatgaa
    26101 ccaactgcaa gaccagcaag gttatcgaat tttgagaaga actcaatggt tttatcgatt
    26161 gtttcgattg gagttgttgt tggaaattgt gttttttcta cgacgttaaa gttttcatca
    26221 ccgacagcac agacaaactt tgtaccgccc gcttccaagc ttccatataa ttttgtcatg
    26281 ataaacctct tgtttttatt ttctttatta tagcatactt cgaaagtcta aatgtctcta
    26341 ttttttagat tttcctctgt aaatcttact atctaataaa aacgaacaaa catgtcattt
    26401 gttcgttttc acattagaga ggattgatta gattttcact tcgatcacag catccccctt
    26461 cgcaactgaa cctgttgcga ctggagctac tgaagcgtag tcagctgtat ttgtaacgat
    26521 aaccattgtt gtatcatcaa gtccagctgc agcgattttg tttgagtcaa atgttccaag
    26581 aacatcgcca gctttcacct tattaccttg agcaactttt gcttcaaaac cgtcaccgtt
    26641 catagataca gtgtcaatac caacgtgaat caaaacttca gcaccatttc ttgttttcaa
    26701 accaaaagcg tgccctgttg gaaaggcaat tgaaacttca gcatcagctg gtgcatagac
    26761 cacgccttgg cttggtttca cagcgatacc ttgtcccata gctccacttg agaagactgg
    26821 gtcattgaca tcagcaagag cgacaacatc accgacgata ggagttacaa gtgtttcatt
    26881 ttgaagagct gctggcgcaa cttcttcttt ttcttcagcc acttcagctt gttttgcagc
    26941 tgcagttgcg tctacttcat cttcgtaacc aaacatgtaa gtaagagcaa aaccaagggc
    27001 aaatgataca gctaccataa gaaggtattg tggaagttgt ccgttaccaa cataaagcat
    27061 tgtaccaggg atgatggtga taccattacc agtaccagca agtccaagga tagaagccaa
    27121 tccaccaccg attgcaccag caatcaatga aaggaagaat ggtttacgga agcgcaagtt
    27181 caccccgaag atagcaggct ctgtaatacc taggaaggca gaaagagcag ccgggaaagc
    27241 aagtgttttc agttttggat tttttgtttt aacaccaacc gcaacagtag cagcaccttg
    27301 agctgtcata gcagctgtga tgatagcgtt gaatgggtta gcatggtcag cagcaagtaa
    27361 ttgcacttca agcaagttga agatgtggtg cacacctgac acgacgatca attggtgaac
    27421 cccaccaatc aagaaaccac caagaccaaa tggaatgcta agaatcgctt ttgtagcaat
    27481 aaggatgtag ttttcaacaa cgtggaaaac tggtccaatg acaaagagtc caaggataga
    27541 catgaccaaa agtgtcacga atggtgttac caagaggtca atgacatctg gaacaacctt
    27601 gcggacagct ttttcaaatt tagctccgac aaccccgatg atgaaggctg gaagaacgga
    27661 accttgcaaa ccaacaacag ggatgaaacc aaagaagttc atcgctgtta cttcaccacc
    27721 ttgagcaact gcccaagcgt ttggaagtga gccagagaca agcatcatac caagaacgat
    27781 accaacggca ggatttccac caaatacacg gaaggttgac cacacaacca aacctggcaa
    27841 gatgatgaag gctgtatctg tcaagatttg tgtgtaagtt gcaaagtcac ctggaagtgg
    27901 catttcaaga gcgttgaaaa gaccacgcac acccatgaag agacctgtcg ctacgataac
    27961 tgggatgatt ggaacgaaaa catcaccgaa agtacggata gcacgttgga accagttccc
    28021 ttgtttagca acttctgctt tcatgtcatc cttagatgat gttggtaagc caagtacaac
    28081 aacttcatcg tacattttgt taactgtacc tgtaccaaag ataatttggt attgccctga
    28141 gttaaagaaa gcaccttgaa ctttttccaa gttctcaatc acttctttat tgattttctc
    28201 ttcatctttg accatgacac gtagacgagt cgcacagtgg gcaacactat tgacattttc
    28261 acgtccgccc aaggcatcga tgactttttt tgcaatttcc tgattgttca tttgcaaaaa
    28321 tctccttata taacattttg ttcttgtttg aaagcgattt tattcgccct tacgactatt
    28381 attttatcat gttttaaaaa tatgtcaagc gttttgcaga aaaatattct atccacttag
    28441 tgggcaagct ttagattgat tgtttttgac tgtttcatca ggaattcctt gaaaaataaa
//
LOCUS       DAVHIT010000022        27529 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVHIT010000022 DAVHIT010000000
VERSION     DAVHIT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.022"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            469..1632
                     /locus_tag="VVN16_000800"
     CDS             469..1632
                     /locus_tag="VVN16_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU5572223.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1629..2306
                     /locus_tag="VVN16_000801"
     CDS             1629..2306
                     /locus_tag="VVN16_000801"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU5572224.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2296..3150
                     /locus_tag="VVN16_000802"
     CDS             2296..3150
                     /locus_tag="VVN16_000802"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU5572225.1"
                     /translation="MKLDGYTRLTAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3169..4236
                     /locus_tag="VVN16_000803"
     CDS             3169..4236
                     /locus_tag="VVN16_000803"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU5572226.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4246..5412
                     /gene="aroC"
                     /locus_tag="VVN16_000804"
     CDS             4246..5412
                     /gene="aroC"
                     /locus_tag="VVN16_000804"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5572227.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5422..6525
                     /locus_tag="VVN16_000805"
     CDS             5422..6525
                     /locus_tag="VVN16_000805"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU5572228.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6536..6874
                     /locus_tag="VVN16_000806"
     CDS             6536..6874
                     /locus_tag="VVN16_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU5572229.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6966..8249
                     /gene="aroA"
                     /locus_tag="VVN16_000807"
     CDS             6966..8249
                     /gene="aroA"
                     /locus_tag="VVN16_000807"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5572230.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSASFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8242..8718
                     /locus_tag="VVN16_000808"
     CDS             8242..8718
                     /locus_tag="VVN16_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU5572231.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8715..9563
                     /gene="pheA"
                     /locus_tag="VVN16_000809"
     CDS             8715..9563
                     /gene="pheA"
                     /locus_tag="VVN16_000809"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU5572232.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSVEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGTYPIYPISD
                     HGKERR"
     gene            9560..10834
                     /gene="psr"
                     /locus_tag="VVN16_000810"
     CDS             9560..10834
                     /gene="psr"
                     /locus_tag="VVN16_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU5572233.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11113..11958
                     /locus_tag="VVN16_000811"
     CDS             11113..11958
                     /locus_tag="VVN16_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU5572234.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDTVIADMDKSYTEYK
                     GEYGG"
     gene            11948..13051
                     /locus_tag="VVN16_000812"
     CDS             11948..13051
                     /locus_tag="VVN16_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5572235.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13053..14039
                     /locus_tag="VVN16_000813"
     CDS             13053..14039
                     /locus_tag="VVN16_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5572236.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14055..15488
                     /locus_tag="VVN16_000814"
     CDS             14055..15488
                     /locus_tag="VVN16_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU5572237.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15517..16551
                     /locus_tag="VVN16_000815"
     CDS             15517..16551
                     /locus_tag="VVN16_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU5572238.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16751..17488
                     /gene="mecA"
                     /locus_tag="VVN16_000816"
     CDS             16751..17488
                     /gene="mecA"
                     /locus_tag="VVN16_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU5572239.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17639..18925
                     /locus_tag="VVN16_000817"
     CDS             17639..18925
                     /locus_tag="VVN16_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU5572240.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18927..19796
                     /locus_tag="VVN16_000818"
     CDS             18927..19796
                     /locus_tag="VVN16_000818"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU5572241.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19872..19946
                     /locus_tag="VVN16_000819"
                     /pseudo
     CDS             19872..19946
                     /locus_tag="VVN16_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20091..21029
                     /gene="msrB"
                     /locus_tag="VVN16_000820"
     CDS             20091..21029
                     /gene="msrB"
                     /locus_tag="VVN16_000820"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5572242.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21087..22811
                     /locus_tag="VVN16_000821"
     CDS             21087..22811
                     /locus_tag="VVN16_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572243.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22813..24561
                     /locus_tag="VVN16_000822"
     CDS             22813..24561
                     /locus_tag="VVN16_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572244.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24863..26122
                     /locus_tag="VVN16_000823"
     CDS             24863..26122
                     /locus_tag="VVN16_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU5572245.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26198..26333
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26372..26872
                     /locus_tag="VVN16_000824"
     CDS             26372..26872
                     /locus_tag="VVN16_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU5572246.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26948..27316
                     /gene="rplL"
                     /locus_tag="VVN16_000825"
     CDS             26948..27316
                     /gene="rplL"
                     /locus_tag="VVN16_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU5572247.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
BASE COUNT         8140 a         5130 c         6201 g         8058 t
ORIGIN      
        1 gtatttttga tagaacatat atttagagtt cgaaatagaa tgatgcaaat aggtgaaatt
       61 ttaattattg ttgcttctat aatattattt attgatagta tattgagttt gtaacaaaag
      121 gcttctctat aaaatcaaat tttccagata tctattgaag cagatttatt tcttcatctt
      181 tcttctacta cctttgtcac taaatttgac agacacatat tgctgataca acctctctac
      241 ctataatctc acagttaacc tgaggctagt ttcatagttt gctatttgat tttcgttgat
      301 aataaaaaat gctcctccaa gacggaagag cattttttat tgtgattctt acacatttac
      361 ttcctagttt tgcaggaagt ctagtatacc attttttaaa agaagagtaa aaaagcaagg
      421 gtagtttctt ttttgagaat tgtctaaaag tttgatataa tggtagttat gaatagaata
      481 agagtcagca aaagggttga aaagaagctt gctaaggggc tagttttact agaagccagt
      541 gatcttgaga atgtcaatct taaggatcag gaagtagagg tgcagggtca ggaaggaaac
      601 tttcttggga ctgcctacct ttctcagcaa aacaagggct tgggctggtt tgtcagcaaa
      661 gacaaggtgg ccttcaatca agctttcttt gaaacgttgt ttagaaaagc caaagaaaag
      721 agaagcgcct actatcaaga tgatttaaca actacctatc gtctctttaa tcaagaggga
      781 gatggctttg ggggtctgac agtggacctt tatggcgact acgccgtctt ttcttggtat
      841 aactcttatg tttatcagat tcgtcagact atatcagaag cctttagaca ggttttccct
      901 gaggttttag gggcttatga gaaaattcgc tttaagggtt tggactatga atctgcccat
      961 gtttatggtc aagaagcacc tgactttttc actgttttag aaaatggtgt cctgtatcaa
     1021 gtctttatga atgatggctt gatgacagga attttcctag accagcatga ggttcgcggt
     1081 agtttagttg acggcttggc tatgggtaaa tccttgctca atatgttttc ctacacagcg
     1141 gctttttcag tagctgcggc catgggagga gctagccaga caacttctgt tgatctagcc
     1201 aaacgttcac gagaattgtc tcaagcgcat tttcaggcaa atgggctcag cacagacgag
     1261 catcgtttta tagtcatgga tgtctttgag tatttcaaat atgccaaacg caaaaacttg
     1321 acctacgatg tgattgtcct agatccgcct agctttgctc ggaataaaaa acaaactttc
     1381 tctgtggcca aggattatca caagttgatt tcccagagtc ttgagatttt aaatccggga
     1441 gggattatca ttgccagtac caatgctgcc aatgtttccc gtcagaaatt tacagaacaa
     1501 attgataaag gctttgcagg aagaagttac cagattttaa acaaatatgg tcttccagca
     1561 gattttgcct ataataaaaa agatgaaagt agtaattacc tcaaggtgat tagtatgaag
     1621 gttagtaaat gaaattaatc gtttcagtaa tgccaagaag tttagaggag gctcaggctc
     1681 tggatgccac gaggtacctg gatgccgaca tcattgaatg gcgtgccgac tatctgccta
     1741 aagaagcgat tttgcaggta gctccagcca tttttgaaaa attcgcaggc cgtgagttgg
     1801 ttttcacgct acgaactcgc tccgaaggtg gagaaatcga cctttctcca gaagagtata
     1861 tccatctaat caaggaagtt gcgcaactct atcaaccaga ctatattgat tttgagtact
     1921 atagctacaa ggatgttttt gaggaaatgc tggacttccc aaatcttgtt ttgagttacc
     1981 ataatttcca agaaacacct gagaacatga tggaaatctt gtcagagttg acgatcctaa
     2041 atccaaaact tgttaaggtt gcggtgatgg ctcacacgga gcaggatgtc ttagacttga
     2101 tgaactatac acgaggcttt aaaaccctca atcctgaaca ggaatatgtg accatttcta
     2161 tgggtaaggt gggcaaggtc tctcgtatca ctgcggatgt gactggttcg agttggtctt
     2221 ttgccagtct ggatgaggtt agtgcccctg ggcagatttc tctagctagc atgaaaaaaa
     2281 tcagggagat tttggatgaa gcttgatggc tatacacgtt taactgcagt tgttgccaat
     2341 cctattaagc attctatttc tcccttcatc cacaatagag cctttgaggc gacagctacc
     2401 aacggtgctt atgtggcttg ggagattgaa gcgagtgact tggcagaaac agtggccaat
     2461 attcgtcgct accagatgtt tggcatcaat ctgtccatgc cctataagga gcaggtgatt
     2521 ccttatttgg ataagctgag cgatgaagcg cgcttgattg gtgcggttaa tacggttgtc
     2581 aatgagaatg gcaatttaat tggatataat acagatggca agggattttt taagtgcttg
     2641 ccttctttta caatttcagg taaaaagatg accctgctgg gtgcaggtgg tgcggctaaa
     2701 tcaatcttgg cacaggctat tttggatggt gtcagtcaga tttcggtctt tgttcgttcc
     2761 gtttctatgg aaaaaacaag accttaccta gacaagttac aggagcagac aggctttaaa
     2821 gtggatttgt gtgctttaga atatgtttct gaactgcaag caaggattgc cgagtcggat
     2881 ttgctagtta atgccaccag tgtgggcatg gatggccaat cctctcctgt tcctgaaaac
     2941 atagtcttac cagaaactct cttagttgca gatatcatat accaaccctt tgaaacaccg
     3001 tttttgaaat gggctagaag tcagggcaat ccagcagtca atggtctggg aatgttactc
     3061 tatcaagctg cagaagcttt tcaactgtgg acaggcaagg aaatgccgac agaagagatt
     3121 tggcagtctt taacagaaaa ataccaataa agaaagggag actctcctat gaaaatcaga
     3181 atcgatattc ctcatcatcc ttatgatatt cagattgaaa aaggttgtat ggcccaggct
     3241 ggtcagtggt tgcgagaact ctggcaaccg caaaaggtag tcattgtgac agataaccat
     3301 gtagcctctc tctatgcaga gaaggtcaag ctcagcctag aagatgctgg ttttcaggta
     3361 gctgtttttg atttcttgga aggtgaagaa agaaagaatt taactactgt tcagaaagtc
     3421 tatgaatttc tagtcaagca aggtctgact cgtagcgatg gaatcgtggc tcttggtggt
     3481 ggcgttgttg gggacctggc tggctttgta gcctctacct atatgcgggg tattcacttt
     3541 gttcagattc cgactagttt gacagctcag gttgattctt ctatcggtgg aaagacaggc
     3601 gttaacacac catttgctaa aaatatggtg gggacctttg ctcaaccaga tggggttctg
     3661 attgatccac ttgttcttga aaccctcgga aaaagagagt tgattgaagg gatgggtgaa
     3721 gttatcaagt atggcttgat tgaggatcca gaactgtggg ctctcttgac ggggctgaat
     3781 ggttctgttg agagcatttt ggaacatgca gagaccttga ttgaacattc ttgtcaggtg
     3841 aagcgcaaga tggtggttga agatgagttg gacaatggta ttcgtcttta cctcaatttt
     3901 ggccacacta ttggccatgc catcgaagcg actgccggtt atggcaaggt catgcatgga
     3961 gaggctgttg ccatgggaat ggtacagatt tccaagattg ctgaggaaaa aggcctcatg
     4021 ccagctggca ttacccaatc tatcacagag atgtgtcaga aattcggctt gcctgttgac
     4081 tatgaaaatt gggaagttga caagctttat caggctctta ctcatgacaa gaaagcgcgt
     4141 ggtaacacct tgaaattggt cttggtgcca gagcttggtt cagcgaccat tcatccagtt
     4201 tctctggaag agatgaaaga ctacttggta aaataaggag agtgtatgag atatttaact
     4261 gcaggagaat cacacggccc ccgtctaaca gctattattg agggaattcc agctggactt
     4321 ccattgacag ctgaggatat caatgaggac cttagacgcc gtcagggtgg ctacggtcgt
     4381 ggtggtcgta tgaagattga gaatgaccag gttgtcttta cttcgggcgt tcgccacggg
     4441 aagacgacag gggcgcctat tactatggat gtcatcaata aggaccacca gaaatggctg
     4501 gacatcatgt ctgcggagga cattgaagac cgtcttaaaa gcaagcggaa aattactcat
     4561 cctcgcccag gtcatgccga tttggttggg gggattaagt accgttttga tgatttgcga
     4621 aattctttgg agcgttcatc agctcgtgaa accaccatgc gggtggcagt tggtgcagta
     4681 gccaaacgcc tcttggctga gctggatatg gagattgcca accatgtcgt ggtctttggt
     4741 ggcaaggaaa tcgatgttcc tgaaaatctg acagtcgctg aaattaagca aagagctgcc
     4801 cagtctgaag tttctattgt caaccaagaa cgagaacagg aaatcaagga ctatattgac
     4861 caaatcaaac gtgatggtga taccatcggt ggggttgtgg agacagtcgt cggaggcgtt
     4921 ccagttggtc ttggttccta tgtccaatgg gatagaaaat tggatgcaag attggctcaa
     4981 gctgttgtct ctatcaatgc ctttaaaggg gtggaatttg gtcttggctt tgaggctggt
     5041 tatcgtaaag gcagccaagt tatggatgaa attctctggt ctaaagaaga cggttatact
     5101 cgccgtacca ataatctagg tggttttgaa ggtggtatga ctaatgggca acccatcgtt
     5161 gttcgtgggg tcatgaaacc cattcctact ctttataaac ctcttatgag tgtggatatc
     5221 gaaacccacg aaccttacaa ggcaaccgtg gagagaagtg atccgactgc tcttccagct
     5281 gcaggaatgg tcatggaagc agttgtagca acggttctgg cgcaagaaat cctcgaaaaa
     5341 ttctcatcag ataatcttga ggaactaaaa gaagcggtag ccaaacaccg agactataca
     5401 aagaactatt aaggagttcc tatggcaaaa acaatctata tcgcaggtct tgggttgatt
     5461 ggagcctcta tggcacttgg tatcaaacgc gatcatccag attatgaaat tttaggttat
     5521 aatcgtagtc aagcttcgag agatatcgcc ttgaaagaag gtatgattga ccgtgcaacg
     5581 gatgattttg ctagttttgc tcctttggca gatgtcatta tcctcagctt gccaatcaaa
     5641 caaactattg ctttcattaa ggagttggcc aatttggatt tgcgagaagg cgttattatt
     5701 tcagatgctg gttcgaccaa gtcaaccatt gtggatgcgg cggagcagta tttggctggc
     5761 aagtctgttc gctttgtcgg ggcccatccc atggctggta gtcacaagac aggggctgct
     5821 tcggcagatg tcaatctttt tgaaaatgcc tattatatct ttacaccttc aagcctgaca
     5881 agtcaggaca cgcttaagga aatgaaggat ctgctttcag gtcttcatgc tcgttttatc
     5941 gagattgatg ccaaggagca tgatcgtgtc acttctcaga ttagccattt tcctcatatt
     6001 ttggcttcta gtctcatgga gcagactgcg gtctatgctc aagagcatga gatggcaagg
     6061 cgctttgcgg caggtggttt tcgagatatg acccgaattg cggaaagcga gccaggaatg
     6121 tggacctcca ttctcttgtc caatagcgag atcattctgg atagaattga ggatttcaag
     6181 gaacgtttgg aagcgattgg tcaggccatt agtaagggag atgaagagca aatttggaac
     6241 ttttttaacc aagcgcgtga acaacgccag accatggaaa tccataagcg tggtggtgtg
     6301 gatagctctt acgacctcta tgttgacgtt cctgatgaag aagatgtcat cctgcggatt
     6361 ttggaactgc tacgtggaac ttccttggtt aatatccaca tcaacgagga aaatcgtgag
     6421 gatattcacg gaatcctaca aatttcattt aaaaatgctc aagacttgga aagagctgag
     6481 catctcataa cagaaaatac cgactataca gtcgtcatca aataaggaga agaacatgtc
     6541 aaatatttac gatagtgcaa acgaactcag tcgcggtcta cgcggattac cagaatacaa
     6601 ggctgttaaa gcagctaaag atgcgattgc agcagatgct gaggcaagca aaatctttac
     6661 agattatctt gcctttcaag aggaaattca aaaactagca cagacaggtc aaatgccaga
     6721 tgcgtccttc caagctaaga tggaaggctt tggtaaacag attcaaggaa atagcctctt
     6781 gtcagaattc tttaccaagc aacaacaatt ggccatttac ctttctgaca ttgaaaaaat
     6841 tgtttttgaa ccagtttcag aattgctgaa ataagaaaag aactatccta aagtcaggaa
     6901 tttttaagga atttctggct ttttatgata aaataggtaa aagtattgca agtatgaggt
     6961 ccagtatgaa actaaaaaca aacattcgcc atttacatgg tagtatccgc gtcccaggtg
     7021 acaagtctat cagccaccgt tccattatct ttggaagttt ggctgagggt gagaccaagg
     7081 tttatgatat tctgcgaggt gaagacgttc tttcgaccat gcaggttttt cgtgaccttg
     7141 gtgttgaaat tgaggataaa gatggggtta ttaccgttca aggtgtaggc atggctggct
     7201 taaaagcgcc gcaaaatgcc cttaatatgg gaaattctgg cacctcgatt cgcctgattt
     7261 caggtgtcct tgctggtgca gatttcgaag tagagatgtt tggagatgat agtctttcca
     7321 aacgtcctat ggaccgtgtg acccttccac tgaaaaaaat gggcgtcagc atttcagggc
     7381 aaactgaacg agacttgcct ccccttcgct taaaagggac gaaaaaccta agacctattc
     7441 attatgagtt gccaattgcc tctgcccaag tcaagtcagc cttgatgttt gcagccttac
     7501 aagctaaggg ggagtcagtt attatcgaaa aagagtgcac ccgtaatcat actgaagata
     7561 tgttgcaaca atttggtggc catttaagtg tggatggcaa gaaaatcaca gttcaaggac
     7621 ctcaaaaact gactggacaa aaggtggtcg taccaggaga tatttccagt gcatcctttt
     7681 ggttagtcgc aggtttgatt gtcccaaatt ctcgtttagt gctgcagaat gtggggatca
     7741 acgaaactcg caccggtatt attgatgtca ttcgtgccat gggtggaaaa ttggaaataa
     7801 ctgaaatcga tccagtcgct aaatctgcta ccttgattgt tgagtcttct gacttgaaag
     7861 gaacagagat tggtggcgct ttgattccac gattgattga tgaattgccc attattgccc
     7921 tgcttgcgac tcaagcccaa ggtgtaacag ttatcaagga tgctgaggag ctcaaggtca
     7981 aggaaacaga ccgtattcag gttgtggcag acgccttaaa tagtatggga gcagatatta
     8041 ctcctacggc agatgggatg attatcaaag ggaaatcagc tcttcacggt gctagagtca
     8101 atacgtttgg tgaccaccgt atcggcatga tgacagctat cgcagccctc ttggttgcag
     8161 atggagaggt ggagcttgac cgtgcagaag ccatcaatac cagctatcct agtttctttg
     8221 atgatttgga gagcttgatt catggctaag gtgttattag ggtttatggg ggctggaaaa
     8281 tcgactattg caagaggctt ggaccctaat taccttgata tggatgctct gattgagaag
     8341 cgcctaggta tgtccattgc gaattttttc gctgaaaagg gagaagcagc ctttcgtcag
     8401 gtagaatcag aagtcctagc tgatttacta caaacagacc aagtcgtgtc aactggagga
     8461 ggagtggtta tttctcagag aaatcgtgac ttactcaaga ctaatacaga taacatctac
     8521 ctgaaagcag attttgaaac cctctaccaa cgtatcgcag ctgataagga caatcagcga
     8581 ccgctttttc taaataatag caaggaagaa ctagtagcta tttttcaaga aagacaggct
     8641 tggtatgagg aagtggctag tcgggttttg gatgtgacca agctaagccc agaggaaatt
     8701 atagaggaac taagatgaaa attgcttatc taggtcccaa gggatcattt tcacaccacg
     8761 ttgtgcagat agcttttcct catgaggaat tgcaggcttt tgccaacatt acagatgtca
     8821 tcaaggctta tgagcaagga ttagtggact attctgtggt gccagttgaa aattctattg
     8881 agggtagtgt tcatgaaacc ttggactatc tttttcatca ggctcacatc caagcagtag
     8941 cagaaatcgt tcagcctatt catcagcagt tgatggtggt tccaggccat actaagattg
     9001 aaaagatttt ttcacatcca caggccttgg ctcaaggaaa gaaattcatc gatgaacaat
     9061 atccagaggc tcaaatcgag gtaacagcta gtacagctta tgcggcccgt tttatttccg
     9121 aacatccaga tcagcccttt gcagcaattg cacctagaag ttctgttgaa gaatatggat
     9181 tggaactgat tgccgaggat attcaggaaa tggaagccaa tttcacacgt ttctgggttc
     9241 taggagctga aaagcctagt attcccttgc aagcacaaac tgaaaagatg agtttggcct
     9301 tgacattacc tgacaacctt ccaggtgcac tttataaggc cctgtcgacc tttgcttggc
     9361 gaggaattga cttgacaaaa attgaaagtc gtccactcaa gacagcactg ggtgaatact
     9421 ttttcattat cgatgtggat tataccgata aggacttggt ccactttgcc caaaaagaat
     9481 tagaagcgat tggaatccag tataaaattc tgggtaccta tcctatttat ccaatatcag
     9541 accatggaaa ggagagaaga tgagtaaaga aaatccctta agtcatcatg agcagttgcg
     9601 ttatgattat ttgctaaaaa atattcacta tctcaatgag agagaaaaaa atgagtttgc
     9661 ctatttgcaa gaaaagctaa ctcttgctag gggaaatagt agctctagct tggaacaaga
     9721 aagagaagag caggttgact taccaagcta tgcgaaccgg agtcgctcac aatccaaatc
     9781 acaagcactc tctttccctc caaaaaagaa aagacggaag ctccgtctta aacgaatttt
     9841 tatggtgatt ttttcacttt tggtctgtgt ggctttggcc atggtattca tgtttttgcg
     9901 tggttaccaa gatgctagcg caaagaaaac tgctgatgcc cgggcagctc aagtagaagt
     9961 ctttaatggt caggacacta gagatggagt taatatttta atcatgggta ctgatggtcg
    10021 aatcggccag aacagtgttg agacacgaac tgactctatt atggtattaa atgtcggggg
    10081 ctcagataag aaaatgaagc tagtcagttt catgcgtgat aatttggtct atatagatgg
    10141 ttatagtcaa gtgattaacg gtagaaaaca gacagataac aagttaaacg tagcctacga
    10201 gttaggagaa caagaggggc aaaaaggggc agaaatggtt cgccaagtct tgaaagataa
    10261 ttttgacttg gacattaagt actacgcctt ggttgatttc caggcctttg ccacagcgat
    10321 tgacacgctt ttccctgatg gggtgacgat tgatgctcaa ttttcaacat tgaatgggcg
    10381 tcccctaaca gaagctacag tcggagatga tttatacgct accgagactg agtctccaac
    10441 ccaaaccatt aaagtcggaa aacagcagat gaatggctcg accctgctca attatgctcg
    10501 tttccgtgat gatgatgagg cggattacgg ccgtacaaaa agacagcaac aagttttaac
    10561 agcaatttta gagcaaatta aagatcccac taaacttttc actggttctg aagctcttgg
    10621 aaaggttttt gctatgacct caaccaacgt accctatact ttcctcctaa caaacggttt
    10681 atctgttttg gatggagcaa aaaatggtat tgaaaaattg acgattccag aactgggtga
    10741 ctgggtagat gcctatgatg tttatggagg cttgggcttg ctggttgatc aaaacaaata
    10801 tcaaaccaag ctcgctcaaa tgggcttgag ataagataat actcaatgaa aatcaaagtg
    10861 caaactagga agctagccgc aggctgctaa aaacacagtt ttgaggttat agataagact
    10921 gacgaagtca gttacctata cctacggcaa ggtgaagctg acgtgctttg aagagatttt
    10981 cgaagagtat aagacagaaa aatcaaagtt tgatagctat ttggatagca gtcaggcttt
    11041 gattttttac agtctgcaat agattttcac cccttgtttg tggtataatg aaacgatacg
    11101 atagaaagaa taatgaacaa tatgactgat ttaaaagcaa ttcaggctcg tagtctggag
    11161 atggctgaat attttgtggc cttttgtaaa gaacatgatt tgctctgtta tctctgtggc
    11221 ggaggcgcta ttggtgccct tcgaaacaag ggatttattc cttgggacga cgacctagac
    11281 ttttttatgc ctcgtaaaga ttatgagaaa ttagcagaat tatggcctcg ttatgcagat
    11341 gaacgttatt tcttgtcaaa gagtcacaag gattttgttg atcgtaatct ttttattacc
    11401 attcgtgaca aggaaaccac ctgtatcaag ccttatcagc aggatttgga tttgccacat
    11461 ggtctggcct tggatgtttt gcctttggat tattatccga aaaatccagc tgagcggaaa
    11521 aaacaggttc gttgggcctt gatttattca ctcttttgtg cgcaaactat tccagaaaag
    11581 catggtgctc tcatgaaatg gggaagtcgc attttactgg gtttgactcc aaaatctctc
    11641 tgttatcgca tctggaaaaa agctgagaaa gaaatgacta agtatgattt ggctgattgt
    11701 gatggcatta cagaattatg ctcaggtcct ggctacatga gaaacaagta cccaatcaca
    11761 tcttttgaag acaatctttt cttgccattt gaaggaacag agatgcctat tccaatcggc
    11821 tatgatgtct atctcagaac tgcttttggg gattatatga cgcctccacc agcagacaag
    11881 caggtaccgc atcatgatac tgtcattgct gatatggata agtcttatac agaatacaag
    11941 ggagaatatg gtggctaaga aaaaaatctt attttttatg tggtcttttt ctcttggagg
    12001 tggtgcagag aagattctat caaccattgt ttcaaatctg gatccagaaa agtatgatat
    12061 tgatattctt gaaatggagc actttgacaa gggatatgaa tctgttccaa agcatgtacg
    12121 cattttaaaa tcccttcaag attatcgcca aaccagatgg ttacgagctt ttttgtggag
    12181 aatgagaatt tattttccaa gactggctcg tcgtttgctt gtaaaggatg attatgatgt
    12241 tgaagtttct tttaccatta tgaatccacc actgttgttc tctaaaagaa gagaagtcaa
    12301 gaagatatct tggattcatg gaagtattga agaacttctt aaggatagct ctaaaagaga
    12361 atcacataga agccagttgg atgctgcgaa tacaattgta gggatttcaa aaaagaccag
    12421 caattctatc aaggaagttt atccagatta tgcttctaaa ttacagacaa tctacaatgg
    12481 atatgatttt cagactattc tagaaaaatc tcaagagaag atcgatatcg agattgctcc
    12541 tcaaagtatc tgtactatcg gacggattga ggaaaataag ggttctgacc gtgtagtgga
    12601 agtgatacga ttattacacc aagagggaaa aaactatcat ctctatttta tcggggctgg
    12661 tgatatggaa gaggaactga aaaaacgagt caaagagtat gagattgagg actatgtaca
    12721 tttccttggt tatcaaaaaa atccttatca gtatttatct cagacgaaag ttctcttgtc
    12781 tatgtctaaa caagaaggct ttcctggagt gtatgtggag gccttgagtc tgggactccc
    12841 ttttatctct acggacgttg gaggggctga ggaattatcc caagaaggac gatttggaca
    12901 aatcattgag agcaatcaag aggcagctca ggcgattact aattacatga cttctgcctc
    12961 aaactttgat gtcgatgagg ctagccaatt cattcaacaa tttacaatta caaaacaaat
    13021 cgaacaagta gaaaaactat tagaggagta gcatggaaac tgcattaatt agtgtgattg
    13081 tgccagtcta taatgtggcg cagtacctag aaaaatcgat agcttccatt cagaagcaga
    13141 cctatcaaaa tctggaaatt attcttgttg atgatggtgc aacagatgaa agtggtcgct
    13201 tgtgtgattc aatcgctgaa caagatgaca gggtgtcagt gcttcataaa aagaacgaag
    13261 gattgtcgca agcacgaaat gatgggatga agcaggctca cggggattat ctgattttta
    13321 ttgactcaga tgattatatc catccagaaa tgattcagag cttatatgag caattagttc
    13381 aagaagatgc ggatgtttcg agctgtggtg tcatgaatgt ctatgctaat gatgaaagcc
    13441 cacagtcagc caatcaggat gactattttg tctgtgattc tcaaacattt ctaaaggaat
    13501 acctcatagg tgaaaaaata cctgggacga tttgcaataa gctaatcaag agacagattg
    13561 caactgccct atcctttcct aaggggttga tttacgaaga tgcctattac cattttgatt
    13621 taatcaagtt ggccaagaag tatgtggtta atactaaacc ctattattac tatttccata
    13681 gaggggatag tattacgacc aaaccctatg cagagaagga tttagcctat attgatatct
    13741 accaaaagtt ttataatgaa gttgtgaaaa actatcctga cttgaaagag gtcgcttttt
    13801 tcagattggc ctatgcccac ttctttattc tggataagat gttgctagat gatcagtata
    13861 aacagtttga agcctattct cagattcatc gttttttaaa aggccatgcc tttgctattt
    13921 ctaggaatcc aattttccgt aaggggagaa gaattagtgc tttggcccta ttcataaata
    13981 tttccttata tcgattctta ttactgaaaa atattgaaaa atctaaaaaa ttacattaga
    14041 acgggggtga ccaagtgatt gatgggaaac gattattatt tagtttgacc atagtcagtt
    14101 atgccttgac gctagtaagt ggaattgtgt atctgtttaa taataataat gttagcttac
    14161 tttctacttt attgttcttg ttggttagta gcttaattgc ttgttggaat gatatcaagt
    14221 attacttaat ccattttatt ttctatttaa ccatttttgt atttctggta tcaagaccga
    14281 ccattgatta ttttagggat ggtgctttgg atacctatca tccaatagcc tatcgttttg
    14341 cctttatagt tgtcatggtt tcgattctgg gcttgaccac aggaggcatt ctggctcgtt
    14401 acttcatagc taggaagaaa ataaaagtag caaatatagg aaattctcta aaagaggttt
    14461 atatcaagcg gttacgcttt gtatcactag gagtttttct tctaacctat cctttctatt
    14521 tcattcggtt atttgaacgg ctcttgtatc gtttgcagac ttcctactat gcctactatg
    14581 caaattttga aagtaaactg ccttatttta cctacatttt gtctaccttt acggtctatg
    14641 caatgtgtat gtatctggca accaagccaa agaaattgca ggccacagca gtgcttgtct
    14701 cctttattgc agctaatact attcatttgg caattgggac acgaaatccc tttattttaa
    14761 gtattttatt tgcttttgtt tattacttta tgcgggagca aactgaaaaa ggaaaatgga
    14821 ttgggtttaa agaaaagtta gcgatttttg taggttctcc tattctcatg ttagcgatgg
    14881 gagtactcaa ttatgtacgg gataatgtcc aagtttctca tacaggtttc tgggatatct
    14941 tacttgactt tatctataaa caagggacta gttttggtgt tctggctcga ggttttctat
    15001 ttaacagtag cctcccttac cgagatttcc gtaattttac ttttggtcct gttcttgatt
    15061 attttgcaag ggggagtttg ggagccattt tcggaggaaa agcctttgaa catacaacca
    15121 atagtgtgga actagctatt gatagtaata gttatgccca caatctatcc tatcttgtct
    15181 tgaacaagga atacttgaaa gggcatggta tcggaagtag ttatatcatg gagttgtata
    15241 ccgactatgg tatgattgga gtctttctgc ttagtttctt actcggcgta ttatttatag
    15301 ccatgctgca agtagcctat cgctcaagga caatcctatt tgctttatcc ctactcatct
    15361 tgaataatct attctttatg ccaagaagca gcttttcaga aagtttcttc aatttattta
    15421 caatgcaatt ctggggaatt gttcttgtga ttatatttgt agcaaaaatg cttacaaagg
    15481 aaaactagta tctactaaac aaaggagaaa aaaatcatgt ttgaacatta ttcagtagct
    15541 gatttgtttg caaatcttta taaaaagcga aaagcaaata ttttagctct catcgcttta
    15601 tttgctctca ttgctgtacc atttacaatt aaagcagtta ggaataaaaa cactgtcaaa
    15661 gatacaacaa gttattcaac ttatcttatc tataaaatca ctcctccaaa agagtcggcc
    15721 aaaacgattt tgaatcatca aattggtggt tatagtgatt tttatgggaa attgattgat
    15781 ggtaatttga atggagctta tcttttcaat gatgtagaac ccagtgagtt gaaaaaaatt
    15841 gccagtgaat tagacacgac agaaacaacc ttgaaaaatt caacgaatga ctattggtgg
    15901 aaaaaattga ccgtctacta tatgattgac gatgcagggg ttggtgtgaa aattttgaca
    15961 tcaagtaaag atgccaatga cttgttagag aaaaaaattg atgggttgat tgagaaattt
    16021 aaacatgcct atgcaaatgt gaaaattgaa aaactggaaa ccatcaactc taaagaattg
    16081 aacgcaaatg gtgaaacagc gcttggctta aatgtgaaaa atctgattct tcgtttagtt
    16141 gttattggag tggtttgtgt gattttggtt gtgatgggaa atgtgttagt ttatctcttt
    16201 aatccaacaa tcaatagagt aggtgatttt tctcagtatc aaattgattt tgtaacagag
    16261 attacaacaa ttgctaacct agcagatgtt ttgtcataca aaaatactgg acaggaattg
    16321 accatcgtta gctcaaataa agctatccta gataaattga aacagagtca agaagcttta
    16381 aaaggaatgc attttgtaga tttacaggat gtatcatctc ttttggaaag agatacagtc
    16441 cttcttgttg aagagtacgg agtgactcgt tataagaaat ttgagcaaag tcttcaaatt
    16501 ctcagaaact taaatcgttc tatccttggt gtagcaacct ttaaattata agtattctga
    16561 tgtataaggt cttcaaaatc ggctaagagc tgattttgaa ggctttttct atctgtctgc
    16621 agaaattttc ttttggagtc tcctatagaa agaccctagg gaaaatctat ccgtatagag
    16681 gagccttctt ttgataaaat cgtaaaaatc tagtataata gatagaactg aaagtatgag
    16741 gttactagca atgaaaatga aacaaattag tgatacaact ttaaaaatca cgatgtcttt
    16801 agaggatttg atggatcgtg gaatggagat tgctgacttt ctcgttcctc aagaaaaaac
    16861 agaagagttc ttttatgcta tcttggatga actagagatg cctgatagct ttctggatac
    16921 aggcatgttg agcttccgtg tgactccaaa acctgataag gtagatgtct ttgtgaccaa
    16981 gtcaaagatt gaccaaaatc tagattttga agacttatcg gatttgccag atatggaaga
    17041 attggctcaa atgtctccag atgaatttat caaaaccctg gaaaaaagca tcgcagacaa
    17101 aaccaaggat gatatcgaag ccattcaatc tcttgagcaa gttgaagcca aggaagaaga
    17161 gcaagagcag gctgaacaag aagctgagag taagaaagaa ccttacatct actacatcct
    17221 ttcttttgct aagttggctg acttggtagt ttttgccaag acagtgactt ttgaaatgga
    17281 aacttctgaa ctctacaaaa tgaacgagcg ctattatttg accattttag tggatattga
    17341 aaatcatcca agcccatatc cagcttggct gttggcccgt atgcgcgagt ttgcagacga
    17401 tagtgatatc agtcgctcag tcttacaaga gtatggtcaa gtcttgatga gtcacgatgc
    17461 agtgctcaat ctgcaaaaaa tcggctaatc ctttctggaa agctattttc agattttaag
    17521 aagagcgaat cgtctgattc gttttttctt tttatactga aatagtgatt tactataata
    17581 ggaattttca caaaattctg ttataatggc tatattagaa aatttcgagg agataaagat
    17641 gacagttaaa attgctttac taggatttgg taccgttgca agtggtgtgc ctttcctcct
    17701 aaaggaaaat ggaggaaaaa tcaatcaatc agcacattca gatatcaaag ttgctaaggt
    17761 attggtcaag gatgaagatg aaaaaaatcg cttgcttgca gcagggaatg actttaactt
    17821 tgtaaccaat gtggatgata ttttatcaga ccaggatatt actatcgtag tggaattgat
    17881 ggggcgtatt gagcctgcta aaacctttat cactcgtgcc ttggaagctg gaaaacacgt
    17941 tgttactgct aacaaggacc ttttagctgt ccatggcgca gaattgctag aaatcgctca
    18001 agctaacaag gtagcacttt actacgaagc agcagttgct ggtgggattc caattcttcg
    18061 tactttagca aattccttgg cttctgataa aattacgcgc gtgcttggag tagtcaacgg
    18121 aacttccaac ttcatggtga ccaagatggt ggaagaaggc tggtcttacg atgatgctct
    18181 tgcggaagca caacgtctag gatttgcaga aagcgatccg acgaatgacg tagatgggat
    18241 tgatgcagcc tacaagatgg ttattttgag ccaatttgcc tttggcatga agattgcctt
    18301 tgatgatgta gcccacaagg gaatccgcaa tatcacacca gaagacgtag ctgtagctca
    18361 agagtttggt tacgtagtga aattggttgg ttctattgag gaaacttctt caggtattgc
    18421 tgcagaagtg actccaacct tcctacctaa agcgcaccca cttgctagtg tgaatggcgt
    18481 aatgaacgct gtctttgtag aatctatcgg tattggtgag tctatgtact acggaccagg
    18541 tgcgggtcaa aaaccaactg caacaagtgt tgtagctgat attgtccgta tcgttcgtcg
    18601 tttgaatgat ggtaccattg gcaaagactt caacgaatat agccgtgact tggtcttggc
    18661 aaatcctgaa gatgtcaaag caaactacta tttctcaatc ttggctccag actcaaaagg
    18721 tcaggtcttg aagttggctg aaatcttcaa tgctcaagat atttccttta agcaaatcct
    18781 tcaagatggc aaagagggtg acaaggcgcg tgtcgttatc atcacacaca agattaataa
    18841 agcccagctt gaaaatgtct cagctgaatt gaagaaggtt tcagaattcg acctcttgaa
    18901 taccttcaag gtgctaggag aataagatga agattattgt acctgcaacc agtgccaata
    18961 tcgggccagg ttttgactcg gtcggtgtag ctgtaaccaa gtatcttcaa attgaggtct
    19021 gcgaagaacg agatgagtgg ctgattgaac accagattgg caaatggatt ccacatgatg
    19081 agcgtaatct cttgctcaaa atcgctttgc aaattgtacc agacttgcaa ccaagacgct
    19141 tgaaaatgac cagtgatgtc cctttggcgc gcggtttggg ttcttccagc tcggttatcg
    19201 ttgctgggat tgaactagcc aaccaactgg gtcaactcaa cttatcagac catgaaaaat
    19261 tgcagttagc gaccaagatt gaagggcatc ctgacaatgt ggctccagcc atttatggta
    19321 atctcgttat tgcaagttct gttgaagggc aagtctctgc tatcgtagca gactttccag
    19381 agtgtgattt tctagcttac attccaaact atgaattacg tactcgcgac agccgtagtg
    19441 tcttgcctaa aaaattgtct tataaggaag ctgttgctgc aagttctatc gccaatgtag
    19501 cggttgctgc cttgttggca ggagacatgg tgaccgctgg gcaagcaatc gagggagacc
    19561 tcttccatga gcgttatcgt caggacttgg taagagaatt tgcgatgatt aagcaagtga
    19621 ccaaagaaaa tggggcctat gcaacctacc tttctggtgc tgggccgaca gttatggttc
    19681 tggcttctca tgacaagatg ccaacaatta aggcagaatt ggaaaagcaa cctttcaaag
    19741 gaaaactgca tgacttgaga gttgataccc aaggtgtccg tgtagaagca aaataaagaa
    19801 tagaagatag gatggggaaa ctcttaacca gagggtttca tatccttttt gtgaaaagaa
    19861 gtttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc tgctcaaaac
    19921 agtgttttga ggttgcagat agaactgacg aagtcagctc aagacactgt tttgaggttg
    19981 cagatagaac tgacgaagtc agtaacatac ctacggtaag gtgacgctaa cgtggtttga
    20041 agagattttc gaagagtatt agttaaaaac gtgataaagg agaaataaag atggcagaaa
    20101 tttatctagc aggtggttgt ttttggggcc tagaggaata tttttcacgc atttctggag
    20161 tgctagaaac cagtgttggc tacgctaatg gtcaagtcga aacgaccaat taccagttga
    20221 tcaaggaaac agaccatgca gaaacggtcc aagtgattta cgatgagaag gaagtgtcac
    20281 tcagagagat tttactttat tatttccgag ttatcgatcc tctatctatc aatcaacaag
    20341 ggaatgaccg tggccgccaa tatcgaactg ggatttatta tcaggatgaa gcagatttgc
    20401 cagctatcta cacagtggtg caggagcagg aacgcatgct gggtcgaaag attgcagtag
    20461 aagtggagca attacgccac tacattctgg ctgaagacta ccaccaagac tatctcagga
    20521 agaatccttc aggttactgt catatcgatg tgaccgatgc tgataagcca ttgattgatg
    20581 cagcaaacta tgaaaagcct agtcaagagg tgttgaaggc cagtctatct gaagagtctt
    20641 atcgtgtcac acaagaagct gctacagagg ctccatttac caatgcctat gaccaaacct
    20701 ttgaagaggg gatttatgta gatattacga caggtgagcc actctttttt gccaaggata
    20761 agtttgcttc aggttgtggt tggccaagtt ttagccgtcc gatttccaaa gagttgattc
    20821 attattacaa ggatctgagc catggaatgg agcgaattga agttcgttct cgttcaggca
    20881 gtgctcactt gggtcatgtt ttcacagatg gaccgcggga gttaggcggc ctccgttact
    20941 gtatcaattc tgcttcttta cgctttgtgg ccaaggatga gatggaaaaa gcaggatatg
    21001 gctatctatt gccttactta aacaaataaa acagagagtg gggcttccca ctttcttcat
    21061 ttctagaata tgaatagaag ggatttatga aacacctatt atcttacttc aaaccctaca
    21121 tcaaggaatc aattttagcc cccttgttca agctgttaga agctgttttt gagctcttgg
    21181 ttcccatggt gattgctggg attgttgacc aatctttacc tcagggagat caaggtcatc
    21241 tctggatgca gattggcctg ctccttatct ttgcagtaat tggcgtttta gtggccttga
    21301 tagctcaatt ttactcagca aaggcagcag taggttttgc taaggaattg acaaacgatc
    21361 tttatcgtca tattctttcc ttgcccaagg acagcagaga ccgtctgaca acttctagtt
    21421 tggttactcg cttgacttcg gatacctacc agattcagac tggtatcaat caattcctgc
    21481 gtctcttttt acgagcgccc attatcgttt ttggtgccat ttttatggct tatcgaatct
    21541 cagctgagtt gactttctgg ttcttagtca tggttgccat tttgaccatt gtcattgtag
    21601 ggttatctcg attggtcaat cctctctaca gtagtctcag aaagaaaacg gaccaactgg
    21661 ttcaggaaac gcgccagcaa ttgcaaggga tgcgggttat tcgtgctttt ggtcaagaaa
    21721 aacgagagtt acagattttt caaaccctta accaagttta tgctagatta caagaaaaga
    21781 caggtttctg gtctagttta ttaacacctc tgacctatct gattgtcaat ggaactcttc
    21841 tcgttattat ctggcaaggc tatatttcaa ttcaaggagg agtgctcagt caaggtgctc
    21901 tcattgctct tatcaattac ctcttacaga ttttggtgga attggtcaag ctagccatgt
    21961 tgataaattc tctcaaccag tcctatatct cagccaagcg aatcgaggag gtctttgcgg
    22021 aatctcccga aaacatccat tcagaattag aacaaaagca agttaccagt ggtcgggttt
    22081 tacaagtcca agaattgact tttacctatc ctgatgcggc ccagccttct ctgagataca
    22141 tttcctttga tatgactcaa ggacaaattc taggtatcat cgggggaacg ggttctggta
    22201 agtcaagctt ggtgcaactc ttacttggac tttatccaat agataagggg agcattgacc
    22261 tttatcgaga tggatctagt cctcgtaatt tagagcagtg gcggtcttgg attgcttatg
    22321 ttccccaaaa ggtcgaactt tttaagggaa ctattcgttc caacttgact ttaggtttaa
    22381 atcaagaagt gactgatcaa gaactctggc aagccttgga aattgctcaa gctaaggatt
    22441 ttgtcagtga taaggaagga ctcttggatg ctctagttga agcaggaggg cgaaatttct
    22501 caggtggaca aaaacaaagg ctgtctatcg cacgagcagt cttgcgccag gctccgtttc
    22561 tcatcctaga tgatgcgacc tcagcactgg acaccattac cgagtccaag ctcttgaaag
    22621 ctattagaga aaattttcca aacatgagct taattttgat ctctcaacga acctcgactt
    22681 tacagatggc tgaccagatt ctcctcttgg aaaaaggtga gttgctagct gttggcaagc
    22741 acgatgactt gatgaaatcc agtcaagtct atcgtgaaat caatgcatcc caacatggaa
    22801 aggaggacta gaatgaaacg acaaactgta aaccagacgc tcaaacgttt agccgtagat
    22861 ttagcaaacc atcccttcct ccttttccta gcctttctag gaactattgc ccaagttggc
    22921 ttatcaattt atctacctat tctgattggg caggtcattg accaagtcct agtggctggt
    22981 tcatcaccag ttttttggca gatttttctc cagatgctct tggtggtaat aggaaatact
    23041 ctagtacaat gggccaatcc tctcctctat aatcgtctaa tcttctctta taccagagat
    23101 ttacgagagc gaatcatcca taaactccat cggttaccga ttgcttttgc ggatcggcaa
    23161 ggcaatggag agatggtcag tcgtgtaacc acggacatcg aacagttggc agctggcttg
    23221 accatgattt ttaaccaatt tttcattggt gttttgatga ttttggttag tattctagcc
    23281 atgctccaaa ttcatctcct catgactctc ttagtcttgc tgttgacgcc actgtccatg
    23341 gtgatttcac gctttattgc caagagatcc tatcatctct tccagaagca aacagagacg
    23401 aggggaattc agactcagtt gattgaagaa tcgcttagcc agcagactat aatccagtcc
    23461 ttcaatgctc aaacagaatt tatccaaaga ttgcgtgagg ctcatgacaa ctactcaggc
    23521 tattctcagt cagccatctt ttattcttca acggtcaatc cttcgactcg ctttgtaaat
    23581 gcactcattt atgccctttt agctggagta ggagcttatc gtatcatgat gggttcaacc
    23641 ttgaccgtcg gtcgtttagt gacttttttg aactatgttc agcaatacac caagcccttt
    23701 aatgatattt cttcagtgct agctgagttg caaagtgctc tggcttgcgt agagcgtatc
    23761 tatggagtct tagatagccc tgaagtggct gaaacaggta aggaagtctt gaccagtgac
    23821 caagttaagg gagctatttc ctttaaacat gtctcttttg gctaccatcc tgaaaaaatt
    23881 ttgattaagg acttgtctat cgatattcca gctggtagta agatagccat cgttggtccg
    23941 acaggtgctg gaaaatcaac tcttatcaat ctccttatgc gtttttatcc cattagctcg
    24001 ggagatatct tgctggatgg gcaatccatt tatgattata cacgagcatc attgagacag
    24061 cagtttggca tggtgcttca agaaacctgg ctcacacaag ggaccattca tgataatatt
    24121 gcctttggca atcctgaagc cagtcgagag caagtaattg ctgctgccaa agcagctaat
    24181 gcagactttt tcatccaaca gttgccacag ggatacgata ccaagttgga aaatgctgga
    24241 gaatctctct ctgtcggcca agctcagctc ttgaccatag cccgagtctt tctggctatt
    24301 ccaaagattc ttatcttaga cgaggcaact tcttccattg atacacggac agaagtgctg
    24361 gtacaggatg cctttgcaaa actcatgaag ggccgcacaa gtttcatcat tgctcaccgt
    24421 ttgtcaacca ttcaggatgc ggatttaatt cttgtcttag tagatggtga tattgttgaa
    24481 tatggtaacc atcaagaact catggataga aagggtaagt attaccaaat gcaaaaagct
    24541 gcggctttta gttctgaata agccattctc ttttgaaagt ttatggacga aaaaagttgc
    24601 cttcgagtga cttttttgtt acaatagcta gaaaaattgt ttactgtaat actcaatgaa
    24661 aatcaaagag caaactagga agctagccgt aggttgctca aaacactgtt ttgaggttgc
    24721 agatagaact gacgaagtca gctcaaaaaa ctgttttgag gttgcagata gaactgacga
    24781 agtcagtaac cacacctacg gcaaagtgaa tttgaagtgg tttgaagaga gtacaacttg
    24841 tcttttagaa aaggagccta taatgaaagt ctttcagcat gtaaatatcg tgacttgtga
    24901 tcaagatttc catgtttatc ttgatggaat cttagcagtc aaggattctc aaatcgtcta
    24961 tgtcggtcaa gataagccag cgtttttaga gcaagctgag cagattatag actatcaggg
    25021 agcttggatt atgcctggtt tggtcaattg tcacacccat tctgcaatga caggcctgag
    25081 agggatccga gatgacagca atctccatga atggctcaat gactatatct ggccagcaga
    25141 atctgagttt actcccgaca tgactaccaa tgcggtcaaa gaagccctaa cagagatgct
    25201 ccagtcagga acaacaacct ttaacgatat gtataatccc aatggtgtgg atatccagca
    25261 aatttatcag gtggtgaaaa cttccaagat gcgttgttat ttttctccga ctctcttttc
    25321 ttcagagaca gaaacaactg ctgagactat aagcagaact cgatccatca tagacgaaat
    25381 cttaaaatat aaaaatccaa atttcaaggt tatggtagca cctcattctc cgtatagctg
    25441 cagtagagac ttgctggaag cgagtttgga aatggcaaaa gagctaaata ttccgctcca
    25501 tgtccatgtg gcggagacca aggaggagtc aggaattatc ctcaaacgat acggcaaacg
    25561 cccccttgct tttcttgaag aactgggtta tttagatcat ccgtccgtct ttgctcacgg
    25621 ggtcgaatta aacgagagag aaattgaacg cttggcatct tctcaagtgg ctatcgccca
    25681 caatcctatc agtaacctca aactggcatc aggaattgct ccaattatcc agctccaaaa
    25741 agcgggagta gcagtcggaa ttgcgactga ctcggttgct tccaataaca atctagatat
    25801 gtttgaggaa ggaaggactg cagctcttct tcagaagatg aaaagtgggg atgccagcca
    25861 gtttccaatc gaaacagctc tcaaggtact gacaatcgaa ggggctaagg tccttggaat
    25921 ggaaaatcag ataggaagtc tggaagtcgg caagcaagca gattttctgg tcattcaacc
    25981 acaagggaaa attcatctcc aacctcagga aaatatgctg tctcacctgg tttatgcagt
    26041 taaatctagt gatgtagatg atgtttatat cgccggagaa caggttgtta agcaaggtca
    26101 agtcctgaca gtagaacttt aaaagaaaaa ttacgaaaaa ttttaaaaaa agtttgcaaa
    26161 aatcttgcat tctttttttg actatgctat acttatatac ggtttaaaaa aactgcctaa
    26221 gacagtaggg gagctcgact cataaatatc ctaccgagga caaaacgtat catgtaaaaa
    26281 gaagcgtatt gtactttcgt gtctaggttt gggcgcgttt ttctttttga aaaattcccc
    26341 aagcaaaata attacggagg tgaacacact aatgagtgaa gcaattattg ctaaaaaagc
    26401 ggaactagtt gacgtagtag ctgaaaaaat gaaagctgct gcatctatcg tcgttgtaga
    26461 cgctcgtggt ttgacagttg agcaagatac agttcttcgt cgtgagcttc gtggaagcga
    26521 agttgagtat aaagttatta aaaactcaat cttgcgtcgt gcagctgaaa aagctggtct
    26581 tgaagatctt gcatctgtat ttgttggacc atctgcagta gcattttcta atgaagatgt
    26641 tatcgcacca gcgaaaatct tgaacgactt ttctaaaaac gctgaagcac ttgaaattaa
    26701 aggtggtgca atcgaaggcg ctgtcgcatc taaagaagag attcttgcac ttgcaactct
    26761 tccaaaccgc gaaggacttc tttctatgct cctttctgta cttcaagcgc cagtgcgcaa
    26821 cgttgctctt gcagtcaaag cggttgcaga aagcaaagaa gacgcggctt aatcttaagc
    26881 tacatagcgt agcctagcta cgaaaaaaac tattataaaa tttaaaactt atttggagga
    26941 aataacaatg gcattgaaca ttgaaaacat tattgctgaa attaaagaag cttcaatcct
    27001 tgaattgaac gaccttgtaa aagctatcga agaagaattt ggtgtaactg cagctgctcc
    27061 tgtagctgtt gctgcagctg atgctgctga tgctggtgct gctaaagatt cattcgacgt
    27121 tgaattgaca tctgcaggcg acaaaaaagt tggtgttatc aaagttgtac gtgaaatcac
    27181 tggtcttggt cttaaagaag ctaaagaact tgttgacggt gcaccagcac ttgttaaaga
    27241 aggcgttgca actgcagaag ctgaagaaat caaagctaaa ttggaagaag ctggagcttc
    27301 agttactctt aaataataga gtatcgcaat ttgattgcgg aatccctatt taccagtctt
    27361 tgagagcgat aagcgattga tagaaactga taaattgatt tggtttcctg ccaaaaattc
    27421 tttggcagga tttttgtttt aataaacttt cgggggtaga cactagattg tctacccctt
    27481 tttttgtgtt tcggaggtaa ctatgaaaag aataattcca gtttatata
//
LOCUS       DAVHIT010000023        27087 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVHIT010000023 DAVHIT010000000
VERSION     DAVHIT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.023"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            165..2798
                     /gene="polA"
                     /locus_tag="VVN16_000826"
     CDS             165..2798
                     /gene="polA"
                     /locus_tag="VVN16_000826"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5572248.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEIKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2883..3320
                     /locus_tag="VVN16_000827"
     CDS             2883..3320
                     /locus_tag="VVN16_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5572249.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(3630..4640)
                     /locus_tag="VVN16_000828"
     CDS             complement(3630..4640)
                     /locus_tag="VVN16_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU5572250.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLTTLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4789..5958
                     /locus_tag="VVN16_000829"
     CDS             4789..5958
                     /locus_tag="VVN16_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5572251.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5955..6725
                     /gene="recO"
                     /locus_tag="VVN16_000830"
     CDS             5955..6725
                     /gene="recO"
                     /locus_tag="VVN16_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5572252.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDASLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6722..7714
                     /gene="plsX"
                     /locus_tag="VVN16_000831"
     CDS             6722..7714
                     /gene="plsX"
                     /locus_tag="VVN16_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU5572253.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTIDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7720..7953
                     /locus_tag="VVN16_000832"
     CDS             7720..7953
                     /locus_tag="VVN16_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5572254.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            7999..8250
                     /locus_tag="VVN16_000833"
                     /pseudo
     CDS             7999..8250
                     /locus_tag="VVN16_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8657..10810
                     /locus_tag="VVN16_000834"
     CDS             8657..10810
                     /locus_tag="VVN16_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU5572255.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTIVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGEMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            10823..12172
                     /locus_tag="VVN16_000835"
     CDS             10823..12172
                     /locus_tag="VVN16_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU5572256.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            12342..13049
                     /locus_tag="VVN16_000836"
     CDS             12342..13049
                     /locus_tag="VVN16_000836"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5572257.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRHINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            13106..16831
                     /locus_tag="VVN16_000837"
     CDS             13106..16831
                     /locus_tag="VVN16_000837"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU5572258.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYAAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            16924..18366
                     /locus_tag="VVN16_000838"
     CDS             16924..18366
                     /locus_tag="VVN16_000838"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5572259.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            18403..19425
                     /locus_tag="VVN16_000839"
     CDS             18403..19425
                     /locus_tag="VVN16_000839"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5572260.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            19422..19967
                     /gene="purN"
                     /locus_tag="VVN16_000840"
     CDS             19422..19967
                     /gene="purN"
                     /locus_tag="VVN16_000840"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU5572261.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            20051..20560
                     /locus_tag="VVN16_000841"
     CDS             20051..20560
                     /locus_tag="VVN16_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU5572262.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWVNILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            20585..22132
                     /gene="purH"
                     /locus_tag="VVN16_000842"
     CDS             20585..22132
                     /gene="purH"
                     /locus_tag="VVN16_000842"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU5572263.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFSDNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            22254..23516
                     /gene="purD"
                     /locus_tag="VVN16_000843"
     CDS             22254..23516
                     /gene="purD"
                     /locus_tag="VVN16_000843"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5572264.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVLHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVIYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNITDILDGKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            23919..24407
                     /gene="purE"
                     /locus_tag="VVN16_000844"
     CDS             23919..24407
                     /gene="purE"
                     /locus_tag="VVN16_000844"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU5572265.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            24394..25485
                     /gene="purK"
                     /locus_tag="VVN16_000845"
     CDS             24394..25485
                     /gene="purK"
                     /locus_tag="VVN16_000845"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU5572266.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSQDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATADDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            25495..25722
                     /locus_tag="VVN16_000846"
     CDS             25495..25722
                     /locus_tag="VVN16_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU5572267.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            25785..27083
                     /gene="purB"
                     /locus_tag="VVN16_000847"
     CDS             25785..27083
                     /gene="purB"
                     /locus_tag="VVN16_000847"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5572268.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
BASE COUNT         7592 a         5317 c         6708 g         7470 t
ORIGIN      
        1 caaggtgtac tattatagat tcaatatact atattatagc acatttttaa atctaggttt
       61 actaaattct ctggctctat ctatttattc ggaaaaagtt tgaaaaatac ttgctctagc
      121 tcttcccaat ggtatttttt gattctttcc tttataatgg gtgtatggat aagaaaaaat
      181 tattattgat tgatgggtct tctgtagctt ttcgggcgtt ttttgcgctg tatcagcagt
      241 tggaccgttt taagaatgcg gctggtttgc ataccaatgc gatttatggt tttcagttga
      301 tgttgagtca tttgttggag cgggttgagc cgagtcatat tttggtggct tttgatgcgg
      361 gaaagacgac cttccggaca gagatgtatg cggactataa gggtggtcgg gccaagactc
      421 ctgatgagtt tcgtgagcaa tttcctttca ttcgtgagtt gctggatcat atggggattc
      481 gtcactatga gctggctcag tatgaggcgg atgacatcat tgggacgctg gataagctag
      541 cagagcagga tggttttgat attaccattg tcagtgggga caaggatttg attcagctga
      601 cggatgagca tacggtggtt gaaatttcca agaaaggtgt ggctgagttt gaggccttta
      661 cgccagatta cctcatggaa gaaatgggcc tcacaccagc tcagtttatc gatctcaagg
      721 cgctcatggg tgataagtcg gataatatcc ctggggtgac caaagtcggt gaaaagacgg
      781 gtattaagct cttgctggag catggttcgc ttgaggggat ttatgaaaat attgatggaa
      841 tgaagacttc taagatgaag gaaaatctca tcaatgacaa ggaacaggcc tttttgtcta
      901 aaacactagc gaccattgat accaaggcac cgattgcgat tggtttagag gacttggtct
      961 atagtggtcc agatgttgaa aatcttggga aattttacga tgagatggga ttcaaacagc
     1021 tcaagcaggc tttaaatgtg tcgtcagctg atgtggctga gggtttggat tttactattg
     1081 ttgaccaaat cagtcaagat atgctgagtg aagagtctat cttccacttt gagctttttg
     1141 gtgagaatta ccatacggat aatttggttg gatttgcctg gtcttgtggg gataagctct
     1201 atgccacaga caagcttgag ctgttgcaag acccgatttt caaggatttc ttagaaaaaa
     1261 catctctgag agtttatgac tttaagaagg ttaaagttct tttgcaacgt tttggtgtgg
     1321 atttgcaggc gcctgctttt gacatccgtt tggctaaata cctcctttcg actgtggagg
     1381 acaatgaaat tgcgaccatc gctagtcttt atggtcagac ttacttggtt gatgatgaaa
     1441 ctttctacgg taagggtgtt aaaaaggcca ttcctgaacg tgagaaattc ttggaacact
     1501 tagcttgtaa acttgctgtt ttggtagaaa cagagcctat tttacttgaa aaactcagcg
     1561 aaaatgggca attagagctt ctttatgata tggagcaacc tctggctttt gtccttgcca
     1621 agatggaaat tgctgggatt atggtcaaga aagagacctt gcttgagatg caggctgaaa
     1681 atgagcttgt cattgaaaaa ctgactcaag agatttacga gctggctggt gaggagttta
     1741 atgtcaactc gcctaagcag ttgggcgtgc ttctctttga gaaattggga cttcctctag
     1801 aatacactaa gaaaaccaag acaggttatt cgacagcagt ggatgtgttg gagcgtctcg
     1861 ctcctattgc tccgattgtt aagaaaatcc tggattaccg tcaaattgct aagattcaat
     1921 ctacttatgt aattggcttg caggactgga ttttggctga tggaaagatt catactcgct
     1981 atatgcagga tttgacccag accgggcgtt tgtctagtgt ggatccaaac ttgcaaaata
     2041 ttcctgcccg attggaacaa gggcgcttga ttcggaaggc ttttgtgcca gagtgggagg
     2101 atagtgtgct actcagctct gactattcac agattgaatt gcgcgttttg gcgcatattt
     2161 ctaaggatga gcacttgatt aaggccttcc aagagggggc agatatccat acttcgacag
     2221 ccatgcgggt ctttggcatt gagcgtcctg ataatgtgac tgcaaatgac cgtcgcaatg
     2281 ccaaggcagt taactttgga gtggtttatg ggatttcaga ctttggcttg tctaataatt
     2341 tgggaattag tcgtaaggaa gccaaagcct acattgatac ctactttgaa cgttttccag
     2401 gtattaaaaa ctacatggat gaagtggtgc gggaggcgcg tgataagggc tatgtagaga
     2461 ccctctttaa gcgtcgccgt gagttgccag atatcaattc gcgcaacttc aatattcgtg
     2521 gttttgcgga gcgaactgct atcaactcac ctatccaggg ttcggcagca gatattctca
     2581 agattgccat gattcagctg gataaagcct tggttgcagg tggttatcag actaagatgc
     2641 tgttacaagt gcacgatgaa atcgtccttg aagtgcctaa atctgaattg gtagagataa
     2701 aaaaattggt gaaacaaacc atggaagaag ccattcaact cagtgttcct cttatcgcag
     2761 atgagaatga aggggcaacc tggtacgagg ctaaataaaa agggggctag tcctcctttt
     2821 ttgtagtaga attctgcaaa ccttttcaaa atgtgctata ctgatgaaaa aggaggattt
     2881 ctatgagtca agaatttatc aatccaagtg atggcgtgat tcgtcagtat ctcgcaacga
     2941 gtaaaaccct tgctgtggtg ggtttgtctg accgtgaaga aacaaccagc aatcgagtaa
     3001 caaaggaaat gcaggctcgg ggctataaaa tcatcccagt caatcccaag gcggcaggtg
     3061 gcgaaatctt gggtgaaaag gcttatgcta gcctcgctga aattcctttt cctgtagata
     3121 ttgtcaatgt ttatcgtcgc agtgaatttc tgcccgatgt ggcgcgtgat tttctcaaag
     3181 ctgatgccaa gattttttgg gcacagctag gacttgaaag tttagaagcg aaagagattt
     3241 tgcgtgatgg tggatgtgat gatatcgtga tgaatcgctg tatcaagaga gaacatactc
     3301 gcttgattga ggaagcataa gaaaaaggta gctgatgggc taccttttgt gttatactca
     3361 atgaaaatca aagagcaaac tagccgcggg ctgctcaaaa caccgttttg aggttgtaga
     3421 tagaactgac gaagtcagct caaaacaccg ttttgaggtt gcagatagaa ctgacgaagt
     3481 cagctcaaaa caccgttttg aggttgcaga tagaactgac gaagtcagct caaaacaccg
     3541 ttttgaggtt gcagatagaa ctgacgaagt cagtaacata tatacggcaa ggcgacgttg
     3601 acgtggtttg aagagatttt cgaagagtat tagaaaatgc cgataagggt ctgcatacca
     3661 agagtggtga ggatgatggc aatccagcag atggctccaa gaagaatgga ttttccactg
     3721 gatttgacca tagcgaccag attggttttg agaccgatag cactcatggc catgacaata
     3781 aggaatttag agagttcttt gagaggggta aagaaactac tggacacacc tagagaggtg
     3841 agtagtgtag tgaggagcga tgcaaggata aagtaaagga taaaaagtgg gaagactttt
     3901 ttcagttgta ggctttgctt gttttcttgt tggcgacttt gccagtagga tagaaagagc
     3961 gtgataggga taatggccaa agtacgtgtg agtttaacaa tggttgcaga ctcgagggta
     4021 ttgctttggt aaagactgtc ccaagcgctg gcggcagccg ttacagagga agtgtcgttg
     4081 accgcagtcc ctgcaaagag ggcgaagcct tcattggata gatgaagcca ggtgccgagg
     4141 gttggaaaga tgagcgcagc caagacattg aagaaaaaga taacggagat ggcttgggct
     4201 acttcctttt ccttagcatg gataacaggc gctgtcgctg caatggcaga acccccacag
     4261 atagaagaac ctactccaat caaggtagtc agttttgtat ccagggcaaa gaagcgctgg
     4321 aagaggtagg caacaatcaa ggctattgaa atggtggaaa ggatgacagg gagtgaagat
     4381 tgcccaactg cgaagacttg agagatattg agaccaaaac caagtaagat aacggcatac
     4441 tggagcaatt tcttggaact aaaggtcaat ccagcatcca gttgtttata agacgagaga
     4501 aagggatgta ggagcatgcc cataaaaatc gcaaaaacgg gcgcgccaat cacagggaag
     4561 aatcctccta agtaccaaga tacgatagaa atgagaaggc agaccaagat gcctgctcca
     4621 ttttttgata gaaatgacat aaaaacctcc aaatagaatc tgttaccatt atagacctgt
     4681 aaacaggaaa agtaaaatag aaagtggaaa gctattctct aatgtatttt tgcggtcggg
     4741 aggcttttgt agtataatag agatacgttt tgaaagtagg aggtatctat ggacttaact
     4801 aagcgcttta ataaacagtt agataaaatt caagtttcgt tgattcgtca gtttgaccag
     4861 gctatttcgg agattcctgg ggtcttgcgt ttgaccttgg gggaacctga ttttacaacg
     4921 ccagaccatg tcaaggaggc ggccaagcga gcgattgatc agaaccaatc ctactataca
     4981 gggatgagtg gtctgctgac tctacgtcag gcagccagtg actttgttaa ggaaaagtac
     5041 caactggact atgctcctga aaatgaaatc ttggttacaa ttggggcgac agaggcttta
     5101 tctgcgactt tgacggctat tttggaagag ggagacaagg tacttttgcc agctcctgct
     5161 tatccaggct atgaaccgat tgttaactta gttggggcag aaattgttga gattgatacg
     5221 actgaaaatg gttttgtctt gactcctgag atgttggaga aggccatttt ggagcagggt
     5281 gataagctca aggcggttat tctcaactat ccagccaatc cgacaggaat tacctacagt
     5341 cgagagcagt tagaggcctt ggcagctgtt ttacgcaagt acgaagtttt tgttgtctgt
     5401 gatgaggttt actcagaatt gacctacaca ggcgaagccc atgtgtctct aggaacgatg
     5461 ttgagagacc aggctattat tatcaatggt ttgtctaaat cgcatgccat gacaggttgg
     5521 cgtttggggc tgattttcgc tccggcgacc ttcacagccc agttaatcaa gagtcaccag
     5581 tacttggtca ctgccgcaaa taccatggcg caacatgctg cggtagaagc cttgacggct
     5641 ggtaaaaacg atgcggagcc catgaagaag gaatatatcc agcgtcggga ctatatcatc
     5701 gaaaaaatga ctgctcttgg ttttgagatt atcaaaccag acggtgcctt ctatattttt
     5761 gctaaaattc cagcgggcta caatcaagac tcctttgctt ttctgaagga ttttgctcag
     5821 aagaaggccg ttgcctttat ccctggtgca gcctttggac gttacgggga aggctacgtc
     5881 cgcctatctt atgcagccag catggagact atcaaagaag ccatgaaacg acttgaggag
     5941 tacatgagag aagcatgatt cagtctatca cgagtcaagg cttggtgctt tacaatcgca
     6001 attttcgtga ggatgacaag ctcgtcaaaa tttttacaga gcaggttggc aaacgcatgt
     6061 tttttgtcaa acacgctggt cagtctaagc tggcgcctgt tattcagccc ttggtgctgg
     6121 cacgatttct cttgcgaatc aatgatgacg gactcagtta catcgaagac tatcatgagg
     6181 tcatgacttt tcccaagatt aatagtgacc tctttgtcat ggcctatgcg acctatgtgg
     6241 cagctcttgc agatgctagt ttacaggaca atcagcagga tgcttccttg tttgcttttt
     6301 tgcaaaagac tttggagttg atggaagcag gcttggatta tcaggttttg accaatattt
     6361 ttgaaattca aattttgact cgatttggaa tcagcctcaa ttttaatgag tgtgtcttct
     6421 gccatcgggt tggtcaggct tttgactttt ctttcaaata tggagcctgc ctctgtccag
     6481 agcattatca tgaggatgag agacgttgcc atctcaatcc caatatcccc tatctgctca
     6541 atcaatttca agctattgat tttgagactt tggagaccat ttcgctcaag cctggaatca
     6601 agcaagagct acgccaattt atggatcaac tatatgaaga gtacgttggg attcacctaa
     6661 aatcaaagaa atttattgat tccctagcag actggggaca attactaaaa gaggaaaaga
     6721 aatgaaaaaa atcgcagtag atgccatggg gggcgattac gcacctcagg ccatcgttga
     6781 gggtgtcaat caagccctat ctgacttttc agatatcgag gttcaactct acggagatga
     6841 agctaaaatc aagcaatatc tgacagcgac agagcgcgtc agcattatcc atacggatga
     6901 gaagattgat tcggatgatg aacctacgag agctattcgg aataagaaaa atgccagtat
     6961 ggtattggca gccaaggctg tcaaagatgg tgaagcagac gctgtccttt cggctgggaa
     7021 tacaggtgct ttgttggctg ctggattctt catcgtgggt cgtatcaaga atatcgaccg
     7081 tcctggactt atgtcaacat tgccgactat agatgggaaa ggttttgaca tgcttgacct
     7141 cggtgctaat gcagaaaata cagcccagca cctccatcaa tacgctgtcc taggttcttt
     7201 ctatgccaag aatgttcgtg gcattgcgca accacgtgtt ggtttgctca acaacggaac
     7261 agagagtagc aagggcgacc cgcttcgtaa ggaaacttat gaattactgg cggctgatga
     7321 aagtttgaac tttatcggaa acgtggaagc gcgtgatttg atgaatggcg ttgcagatgt
     7381 tgttgtggca gatggtttca cgggaaacgc tgtgctcaaa tccatcgaag ggacagctat
     7441 gggaatcatg ggcttgctca agacagctat tacaggtggt ggtcttcgag cgaaactagg
     7501 tgccctcctt ctcaaggaca gcctcagagg tttgaaaaaa cagctcaatt attcagatgt
     7561 tggtggagcg gtcttgtttg gtgttaaggc acctgttgtc aagactcatg gctcaagcga
     7621 tgccaaggct gtttatagta cgattcgcca gattcgtacc atgctagaaa cagacgtagt
     7681 tgcccagact gcgcgtgaat tttcaggaga ataaaagaga tgacagaaaa agaaattttt
     7741 gaccgtattg tgaccattat ccaagagcga cagggagagg actttgtcgt gacagaatcc
     7801 ttgagtctga aagacgattt ggatgcggat tctgttgact tgatggagtt tatcttgact
     7861 ctggaagatg aatttagtat cgaaatcagc gatgaagaaa ttgaccaact ccaaaacgta
     7921 ggagatgtgg ttaaaatcat tcaaggaaaa tagcaatcgg agttccaagt caacggaagt
     7981 agatggtttt tagaaatgag aaatatcgga caagctggta aaatcttggc tgacagtggt
     8041 tatcaagggc tcatgaagat atatcctcaa gcacaaactc cgaggaaatc aagtaaactt
     8101 aagccattaa ctcttgaaga caaagcctgt aaccatgcac tatctaagga gagcagcaag
     8161 gttgagaata tttttgctaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat
     8221 catcgtaaac gcttcggatt tagaaattga agtaataaat aggatgtcac aaatgttgct
     8281 atcaatgatt tatttgttcc aagcttgcct agggtgtcag taaaaaatca ttttcctttc
     8341 aatagcatat ttttagtggg caggactctt gttctgccta tttttttatc caaaaagtgc
     8401 agttgggagg gagataggcg catttgggaa ggaagtccag tttttgttta gtgattgggg
     8461 taagatagtt gttatcagat gagtttatac tcttcgaaaa tcaaattcaa accacgtcaa
     8521 cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc tatctacaac ctcaaaacag
     8581 tgttttgagc agcctgcggc tagtttccta gtttgctctt tgattttcat tgagtattag
     8641 ggaaaaggag atgaatatga aatttgggaa acgtcactat cgtccgcaag tggatcagat
     8701 ggactgcggt gtagcttcat tagccatggt ttttggctac tatggtagtt attatttttt
     8761 ggctcacttg cgagaattgg ctaagacgac catggatggg acgacggctt tgggcttggt
     8821 caaggtggca gaggagattg gttttgagac gcgagccatt aaggcggata tgacgctttt
     8881 tgacttgccg gatttgactt ttccttttgt tgcccatgtg cttaaggaag ggaaattgct
     8941 ccactactat gtggtgactg ggcaggataa ggatagcatt catattgccg atccagatcc
     9001 cggggtgaag ttgactaaac tgccacgtga gcgttttgag gaagaatgga caggagtgac
     9061 tctttttatg gcacctagtc cagactataa gcctcataag gaacaaaaaa atggtctgct
     9121 ctcttttatc cctatattag tgaagcagcg tggcttgatt gccaatatcg ttttggcaac
     9181 actcttggta accgtgatta acattgtggg ttcttattat ctgcagtcta tcattgatac
     9241 ctatgtgcca gatcagatgc gttcgacact agggattatt tctattgggc tagtcatcgt
     9301 ctacatcctc cagcaaatct tgtcttacgc tcaggagtat ctcttgcttg ttttggggca
     9361 acgcttgtcg attgacgtga ttttgtccta tatcaagcat gtttttcacc tccctatgtc
     9421 cttctttgcg acacgcagga caggggagat cgtgtctcgt tttacagatg ctaacagtat
     9481 catcgatgcg ctggcttcga ccatcctttc gattttccta gatgtgtcaa cgattgtcat
     9541 tatttccctt gttctatttt cacaaaatac caatctcttt ttcatgactt tattggcgct
     9601 tcctatctac acagtgatta tctttgcctt tatgaagccg tttgaaaaga tgaatcggga
     9661 taccatggaa gccaatgcgg ttctgtcttc ttctatcatt gaggacatca acggtattga
     9721 gactatcaag tccttgacca gtgaaagtca gcgttaccaa aaaattgaca aggaatttgt
     9781 ggattatctg aagaaatcct ttacctatag tcgagcagag agtcagcaaa aggctctgaa
     9841 aaaggttgcc catctcttgc ttaatgtcgg cattctctgg atgggggctg ttctggtcat
     9901 ggatggcaag atgagtttgg ggcagttgat tacctataat accttgctgg tttactttac
     9961 caatcctttg gaaaatatca tcaatctgca aaccaagctt cagacagcgc aggttgccaa
    10021 taaccgtcta aatgaagtgt atctagtagc ttctgagttt gaggagaaga aaacagttga
    10081 ggatttgagc ttgatgaagg gagagatgac cttcaagcag gttcattaca agtatggcta
    10141 tggtcgagac gtcttgtcgg atatcaattt aaccgttccc caagggtcta aggtggcttt
    10201 tgtggggatt tcagggtcag gtaagacgac tttggccaag atgatggtta atttttacga
    10261 cccaagtcaa ggggagatta gtctgggtgg tgtcaatctc aatcagattg ataaaaaagc
    10321 cctgcgccag tacatcaact atctgcctca acagccctat gtctttaacg gaacgatttt
    10381 ggagaatctt cttttgggag ccaaggaggg gacgacacag gaagatatct tacgggcggt
    10441 cgaattggca gagattcgag aggatatcga gcgcatgcca ctgaattatc agacagaatt
    10501 gacttcggat ggggcaggga tttcaggtgg tcaacgtcag agaatcgctt tggcgcgtgc
    10561 tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg actagcagtt tggatatttt
    10621 gacagagaag cggattgtcg ataatctcat ggctttggac aagaccttga ttttcattgc
    10681 tcaccgcttg actattgctg agcggacaga gaaggtggtt gtcttggatc agggcaagat
    10741 tgtcgaagaa ggaaagcatg ctgatttgct tgcacaggga ggcttttacg cccatttggt
    10801 caatagctag aaagaggaga ggatgaaacc agaattttta gaaagtgcgg agttttataa
    10861 tcgtcgttac cataattttt ccagtagtgt gattgtaccc atggcccttc tgctcgtgtt
    10921 tttacttggc tttgcaactg ttgcagagaa ggagatgagt ttgtccacta gagctactgt
    10981 cgaacccagt cgtatccttg caaatatcca gtcaactagc aacaatcgta ttcttgtcaa
    11041 tcatttggaa gaaaataagc tggttaagaa gggggatctt ttggttcaat accaagaagg
    11101 ggcagagggt gtccaagcgg agtcctatgc cagtcagttg gacatgctaa aggatcaaaa
    11161 aaagcaattg gagtatctgc aaaagagcct gcaagaaggg gagaaccact ttccagagga
    11221 ggataagttt ggctaccaag ccacctttcg cgactacatc agtcaagcag gcagtcttag
    11281 ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag aatgcagcag ctagccaaac
    11341 ccaagccgaa atcggcaacc tcatcagtca aacagaggct aaaattcgcg attaccagac
    11401 agctaagtca gctattgaaa caggtgcttc cttggccggt cagaatctag cctactctct
    11461 ttaccagtcc tacaagtctc agggcgaaga aaatccccaa actaaggttc aggcagttgc
    11521 acaggttgaa gcacagattt ctcagttaga atctagtctt gctacttacc gtgtccagta
    11581 tgcaggttca ggtacccagc aagcctatgc gtcagggtta agcagtcaat tggaatccct
    11641 taaatcccaa cacttggcaa aggttggtca ggaattgacc cttctagccc agaaaatctt
    11701 ggaggcagag tcaggtaaga aggtacaggg aaatctttta gacaagggga aaattacggc
    11761 gagtgaggat ggggtgcttc atcttaatcc tgagaccagt gattctagca tggttgcaga
    11821 aggtgcccta ctagcccaac tttatccatc tttggaaaga gaaggtaaag ccaaactcac
    11881 agcttatcta agttcaaaag atgtagcaag aatcaaggtc ggtgattctg ttcgctatac
    11941 tacgactcat gatgccggga atcaactttt cctagattct actattacaa gtattgatgc
    12001 gacagctact aagactgaga aagggaattt ctttaaaatc gaggcggaga ctaatctaac
    12061 ttcggagcag gctgaaaaac ttaggtacgg ggtggaaggc cgcttgcaga tgattacggg
    12121 caagaaaagt tacctacgtt attatttgga tcaatttttg aacaaagagt aatgttcgtg
    12181 tttttagagt taaataattt ttaaactgtg agaaagattc ttcttgcagt tttttcttta
    12241 caatttttga aaaacatctg ctatttattc ggttaaattc ttgtgttttt tggttttttg
    12301 tggtaaaatg tgctcaagta atacgaaagg cgaactttaa aatgtcaaaa caattgatct
    12361 attcgggaaa agctaaagac atctatacaa ctgaggatga aaatcttatt atttcaactt
    12421 acaaggacca ggcgactgct ttcaacggtg tcaagaagga gcagattgca ggtaagggag
    12481 tcttgaataa tcagatttca tcttttattt ttgagaaatt aaatgcggct ggtgtggcga
    12541 ctcactttgt ggagaaactt tcagacacgg aacaactcaa taaaaaggtt aagattattc
    12601 ctttggaagt cgtgctccgc aactatactg ctggttcctt ttcaaaacgt tttggtgtgg
    12661 atgagggaat cgccttggag actccgattg tcgaatttta ctacaaaaat gatgatttgg
    12721 atgatccatt tatcaatgat gagcatgtga aattcctaca gattgcgggt gaccagcaga
    12781 ttgcctactt gaaggaagaa acgcgtcata tcaatgaact attgaaagtc tggtttgctg
    12841 agattgggct taaattgatt gactttaagc tagagttcgg ttttgacaag gatggcaaga
    12901 ttatcttggc agacgaattt tcaccagata actgccgctt gtgggacgct gatggcaacc
    12961 acatggataa ggatgttttc cgtagaggat tgggagaact aaccgacgtt tatgagattg
    13021 tttgggaaaa gttgcagggt ttaaaataac aacctcaagg ctgtttggga atattgcaag
    13081 agctgaaata aaggaataag aattgatgga taaacgtatt tttgttgaaa aaaaggctga
    13141 ttttcaggtc aagtcagaga gtttggttag agagctccag cacaacttgg gactgtcaag
    13201 cttgaaaagt attcgtattg tgcaagtata tgatgtattt gacttggttg aggacttgtt
    13261 tgcacctgca gagaagcaca ttttctctga gcaggtaacc gaccatgttt tagatgaagt
    13321 atctgtgcag gcggatcttg ctaactatgc tttctttgcc attgaaagtc tgccagggca
    13381 gtttgaccag cgtgcagctt cgtcacagga agccttgctt ttgttgggaa gttcgagtga
    13441 cgtgacagtc aacacagccc aactttactt ggtgaataaa gatattgatg cgactgagtt
    13501 ggaagctgtc aaaaactacc tgctcaatcc agttgattct cgtttcaagg atatcacgac
    13561 agggattgcc aagcaggagt tttcagagtc agacaagacc attcccaaat tgactttctt
    13621 tgaaagctat gcagcagaag actttgctcg ctacaaggcc gaacaaggga tggccatgga
    13681 agtggatgat ttgctcttta tccaagacta ctttaagtca atcgggcgcg tgccaactga
    13741 gactgaactc aaggttttgg acacttactg gtctgaccac tgccgtcata cgacttttga
    13801 gacagagttg aaacacatcg acttttcagc ttctaaattt caaaagcaat tgcagtcaac
    13861 ctatgacaag tatattgcca tgcgcgagga attaggtcgg tctgaaaaac cacaaacctt
    13921 gatggatatg gcgactattt tcggtcgtta tgagcgtgct aatggacgat tggatgatat
    13981 ggaagtctct gacgaaatca atgcctgctc agttgaaatt gaagtggacg ttgatggtgt
    14041 caaggaacct tggctcctca tgtttaaaaa cgaaacccac aaccatccaa cagaaattga
    14101 gccatttggt ggagcggcta cctgtattgg tggagctatt cgtgatccgt tgtcaggccg
    14161 ttcctatgtt taccaagcca tgcgtatttc aggtgctggt gatattacag caccgatttc
    14221 ggaaactcgc gctgggaaat tgccacaaca agtcatttct aaaacagcaa ctcatggtta
    14281 ttcttcatat ggtaaccaga ttgggcttgc cacaacctac gttcgtgaat acttccaccc
    14341 aggctttgta gctaaacgta tggaacttgg tgccgttgtt ggtgcggctc ccaagggcaa
    14401 tgttgtccgt gaaaaacctg aagcaggtga tgtgatcatc cttctcggag gtaaaacagg
    14461 tcgtgatggt gtcggtggtg cgacgggctc ttctaaggtt caaacagttg agtctgtaga
    14521 gactgctggt gctgaggttc aaaaaggaaa tgccatcgaa gaacgcaaga ttcagcgcct
    14581 cttccgtaat ggcaatgtca ctcgtctgat caagaagtcc aatgactttg gggcaggcgg
    14641 tgtctgtgtg gctatcggtg aattggcaga cggtcttgaa atcgacctca acaaggtgcc
    14701 tcttaaatac cagggcttga atggtacaga aattgccatc tctgaatcac aagaacggat
    14761 ggcggtcgtg gttcgtcctg aagatgtgga taccttcgtt gccgaatgta acaaagaaaa
    14821 tattgatgct gttgtggtgg cgacagtaac tgaaaaacca aatcttgtca tgcactggaa
    14881 tggtgagaca atcgttgact tggagcgtcg tttccttgac accaatggtg tgcgcgtggt
    14941 tgtcgatgcc aaagttgtgg acaaggatgt caaactccca gaagagcgtc aaacatctgc
    15001 tgaaacactg gaatcagata cccttacggt tctatctgac ctcaaccatg caagtcaaaa
    15061 aggattacag actatctttg actgctctgt tggacgctca acggttaatc acccacttgg
    15121 tggtcgttac caactcacac caactgaggc atctgtgcag aaattgccag ttcaacacgg
    15181 tgtgactcat actgcgtcgg tcattgctca aggtttcaac ccatatgtag ctgaatggtc
    15241 tccataccac ggtgctgctt atgcggttat cgaagcaact gctcgtttgg tggctgctgg
    15301 tgccaactgg tctaaggctc gtttctctta ccaagaatat ttcgagcgga tggacaagca
    15361 agcagagcgt ttcggtcagc cagtagctgc tcttctaggt tctattgaag cacaaattca
    15421 gcttggcttg ccatctatcg gtggtaagga ctccatgtct ggtacctttg aagaattgac
    15481 cgttccgcca accttggttg cctttggggt gacgacggca gatagccgta aggtgctctc
    15541 tccagaattt aaagctgttg gggaaaatat ctactacatc ccaggtcaag ccctctctgc
    15601 agagattgat tttgacttga ttaagaaaaa ttttgctcag tttgaagcaa gccaagctga
    15661 ccataaagtg acgtctgcat cagctgtcaa atacggtggt gtagttgaaa gtttggctct
    15721 tgctaccttt ggaaactata ttggtgcaga ggtgaccttg cctgaactta aaacagcttt
    15781 gacagctcaa ttaggcggct ttgtcttcac atctcctgaa gaaattgctg gagtagagaa
    15841 gattggacaa acgaaagcag actttacact gactgtcaac ggtgtgaagc tagatggaca
    15901 caagcttgac agtgcatttc aagggacatt ggaagaagtt tacccaacag aatttaccca
    15961 agcgaaagaa ctagaagaag taccagctgt ggcatcagat gttgtgatta aagccaaaga
    16021 aaaggttgaa aaacctgtgg tttacatccc agtctttcca ggaaccaact cagaatatga
    16081 ttcagctaag gccttcgaaa aagaaggtgc agaggtcaat ttggtgccat tcgtgacctt
    16141 gaatgaagaa gctattgtca agtcagttga aactatggtt gacaatatcg acaagactaa
    16201 tattctcttc tttgccggtg gattctcggc tgcggatgaa ccagatggtt cagctaagtt
    16261 tatcgtcaat atcctgctta atgaaaaagt gcgtgtggct attgatagct ttatcgcccg
    16321 tggtggcttg attatcggta tttgtaatgg attccaagcc ttagtcaaat cgggtctcct
    16381 accatacgga aactttgaag ctgctaacaa tactagccca accctcttct acaatgatgc
    16441 caaccaacac gtggccaaga tggtggaaac tcgcattgcc aataccaact caccatggtt
    16501 ggctggtgtg caagtgggcg atatccacgc tattcctgtt tcgcacggtg aagggaagtt
    16561 tgtcgtgacg gctgaggaat ttgcagagct ccgtgacaat ggacaaattt tcagccaata
    16621 cgttgacttt aacggtaaac caagtatgga ttctaagtac aatccgaatg gttctgtcca
    16681 tgccatcgaa ggaattacca gcaagaatgg tcaaatcatc ggtaagatgg gccactcaga
    16741 acgttatgag gatggtcttt tccaaaatat cccaggcaat aaagaccaac acctgttcgc
    16801 atcagcggtt aaacatttca ctggaaaata agacttacag attttctaat agatagtatc
    16861 agtaatgtaa aagtcatgta aatctagctc ttgatgatta caaatgaaaa ttaggtataa
    16921 aaaatgacat acgaagtaaa atctcttaat gaagaatgtg gtgttttcgg tatttgggga
    16981 catccagatg ctgctaagtt gacctatttt ggactccaca gtcttcaaca ccgtggtcag
    17041 gagggggcag gaatcctctc caatgatcaa ggacaactga agcgccatcg tgacatgggg
    17101 cttttatcag aagttttcag aaatccagcc aatttggata aattgacagg agctggtgcg
    17161 attgggcatg tgcgttatgc gactgctggc gaagcttctg ttgataacat ccagcccttc
    17221 ctcttccgtt ttcacgatat gcagtttggc ttggctcata atggaaatct gaccaatgca
    17281 gcctctctca agaaagaact ggaacaaaga ggagcaattt tcagcgcgac ttcggactct
    17341 gaaatcttgg ctcacctcat tcgtcgtagt cataatccta gcctgatggg caaaatcaag
    17401 gaagcgctca gccttgtcaa aggtggtttt gcctatatct tgctgtttga ggacaagttg
    17461 attgcggctc ttgaccctaa tggctttcgt ccgctttcta tcgggaaaat ggccaacgga
    17521 gcggtggttg tttcctctga aacctgtgct tttgaggtca ttggtgccga gtggattcgt
    17581 gatttgaagc caggtgagat tgtgatcatt gatgacgagg gcattcagta tgacagctat
    17641 acagatgata ctcagttggc gatttgttct atggagtata tctactttgc tcgccctgat
    17701 tctaatatcc acggtgtcaa tgtccatacg gcacgtaaga gaatgggagc acaattggcg
    17761 cgagaattta agcatgaggc agatattgta gttggtgtgc ccaattcttc cctaagcgcg
    17821 gctatgggat ttgcggaaga atcaggctta ccaaatgaaa tgggtctgat caaaaaccaa
    17881 tacacccagc gaacttttat ccaaccgact caagaattgc gggagcaagg agtgcggatg
    17941 aaactgtctg ctgtttcggg tgttgtcaaa ggcaaacgtg tggtcatggt ggatgattcc
    18001 attgtacgtg gaacaacctc tcgtcgtatc gttcagctct tgaaagaagc gggtgcgact
    18061 gaggttcacg ttgccattgg aagtcctgca ctagcgtatc catgtttcta cgggattgat
    18121 atccagaccc gtcaggagct gattgcagcc aatcatacgg tcgaagaaac tcgccaaatc
    18181 attggtgcgg acagtctgac ttatctttca attgatggct tgattgagtc gattggtatc
    18241 gaaacagatg cgccgaacgg tggtctctgt gtcgcttact ttgacggtga ctacccaacg
    18301 cctctttatg actacgaaga agactatcgt agaagtttgg aagaaaagac cagtttttac
    18361 aagtaggcga cagattctcc attaaagaaa aggaaaaaac aaatgacaaa taaaaatgca
    18421 tatgcccagt cgggtgtgga tgttgaagcg ggttatgaag ttgttgaacg gattaaaaag
    18481 cacgtggccc gtacggagcg tgcaggtgtc atgggagctc ttggtggctt tggtggtatg
    18541 tttgaccttt ccaagactgg ggttaaagaa cccgtcttga tttcagggac tgacggtgtc
    18601 ggaaccaagc tcatgttggc tatcaagtac gacaagcacg ataccatcgg gcaggactgt
    18661 gtggccatgt gtgtcaacga catcattgct gcaggtgcgg aacccctcta ttttctcgac
    18721 tacgtagcga cagggaagaa tgaaccagct aagctagaac aagtggttgc tggtgtggca
    18781 gaaggttgtg tgcaggctgg tgctgccctc atcggtgggg aaacggctga aatgccgggc
    18841 atgtacggcg aagacgacta tgacttggct ggttttgcgg tcggtgtggc tgaaaaatct
    18901 caaatcattg acggttcaaa ggtggtagag ggagatgttc ttctcggact tgcttcaagt
    18961 gggattcact caaatggtta ctctttggtt cgtcgtgtct ttgcggatta cacaggtgag
    19021 gaagtcctac cagaattgga aggcaagaaa cttaaggaag ttctacttga gccgactcgt
    19081 atctatgtca aggctgtctt gccgctcatc aaagaagagt tggtcaacgg cattgcccac
    19141 atcacaggtg gtggctttat cgaaaatgtc cctcgtatgt ttgcagatga cctagctgct
    19201 gaaattgatg aaagcaaagt tccagtgctt ccaattttca aagcccttga aaaatacggt
    19261 cagattaaac acgaagaaat gtttgaaatc ttcaatatgg gtgtgggact catgttggcg
    19321 gtcagccctg aaaatgtaga gcgtgtaaaa gaattgttgg atgaagcagt ctatgaaatt
    19381 ggtcgcatcg tcaagaaaga aaacgaaagt gtcattatca aatgaaaaaa atagcggttt
    19441 ttgcctctgg taatggctca aattttcagg tgattgccga agaatttcca gtggagtttg
    19501 tcttttcaga ccatcgtgat gcctatgtgc ttgagcgtgc aaagcagctc ggcgttctgt
    19561 cctatgcttt tgaactcaag gagtttgaga gcaaggcaga ctacgaagca gcccttgtcg
    19621 aactcttgga agaacaccag attgacttgg tttgcctagc aggctacatg aaaatcgttg
    19681 gaccaacctt attgtcggct tatgaaggtc ggattgtcaa cattcatcca gcctacttgc
    19741 cagaatttcc aggagctcat gggattgagg atgcttggaa tgctggcgtg ggtcagtctg
    19801 gtgtgaccat tcactgggtg gattcgggtg tggatacagg ccaggtcatc aaacaggttc
    19861 gtgtgccacg actagctgat gataccattg acagatttga agctcgcatc catgaagcag
    19921 agtacaggtt gtatccggaa gtagtgaagg ctctatttac agattgactt tttgatgatt
    19981 catatgatat ctttgatttt aaattggagt cagtgtttgt tgaagacggc ttcaaacgga
    20041 ggtatttgta atgttagaat ctaaaaaaac aactcgatat gtattttatg tctatctgat
    20101 gttattaact tggggaatct tatttaagtt tgaaacaaat cctgaattta tagcattttt
    20161 cttagctcca aggtatatca attggattcc attttcagaa ccactaatag tcgatggaaa
    20221 aattgttttt gctgaaatgt tatttaatct gatttccttt attccattag gtgtttgttt
    20281 ccctttgata aaaactaatt tatctagttt aagaatagtc gggacaggtt tcttgattag
    20341 tttattgttt gagtgcttac agtatatttt agcaataggt ataacagata taacggattt
    20401 gactttaaat acgctaggtg tctgtgtagg cttactgatt tatcaaattt ttataagagt
    20461 gttcaaatca cagactagaa aatgggtcaa tatcttaggt atgcttagcc ttggttttgc
    20521 ttatcttgtt ttactgttac tgcatttaat tggtgtttaa ctaatgatta aaaaggagaa
    20581 tataatgact aaacgcgtct taatcagcgt ctcagacaaa gcgggcattg ttgaatttgc
    20641 ccaagaactc aaaaaacttg gttgggagat tatctcaaca ggtggaacta aggttgccct
    20701 tgataatgct ggggtggata ccattgctat cgatgatgtg actggtttcc cagaaatgat
    20761 ggacggtcgt gtgaagaccc tccacccaaa tatccacgga gggcttctcg ctcgtcgtga
    20821 cttggatagc cacttggaag cggctaagga caacaagatt gagctcattg accttgtggt
    20881 ggtcaacctt tacccattta aggaaactat ccttaaacca gatgtgactt atgctgatgc
    20941 agttgaaaat atcgatattg gtgggccgtc tatgcttcgt tcagcagcga aaaatcatgc
    21001 cagtgttaca gttgtggtag atcctgctga ctacgctgtg gttttggacg aattggcagc
    21061 aaacggcgaa acctcttatg aaactcgcca acgtttagca gccaaagtat ttcgtcacac
    21121 agcggcttat gacgccttga ttgcagaata cttcacagct caagtgggtg aaagcaagcc
    21181 tgaaaaactc actttgactt atgacctcaa gcaaccaatg cgttacggtg agaatcctca
    21241 acaagacgcg gacttttacc agaaagcttt gcctacagat tactctattg cttcagccaa
    21301 acagctcaac gggaaagaat tgtcatttaa taatatccgt gatgcagatg ctgctatccg
    21361 tatcatccgt gacttcaaag atagtccaac cgttgtggct ctcaaacaca tgaatccatg
    21421 tggaattggt caagctgatg acatcgagac tgcttgggac tacgcttatg agtctgaccc
    21481 agtatctatc tttggtggga ttgtcgtcct caaccgtgag gtggatgctg cgacagctga
    21541 gaagatgcac ggcgttttcc tcgaaatcat cattgcacca agctatacgg atgaagcgct
    21601 agccattttg atcaataaaa agaaaaactt gcgtatcctt gccttgccat ttaatgctca
    21661 agaggctagc gaagtggaag cagaatacac aggtgtagtc ggtggacttc tcgtgcaaaa
    21721 tcaagacgtg gtcaaggaaa gcccagctga ctggcaagtg gtgaccaaac gtcagccaac
    21781 cgagacagaa gcgactgctc ttgagttcgc ttggaaggct atcaagtacg tcaaatcaaa
    21841 tggtattatc gtgaccaacg accacatgac acttggtgtt ggtccaggtc aaaccaaccg
    21901 tgtggcttct gttcgccttg ccattgacca agccaaagat cgtctgaacg gggcggtcct
    21961 tgcttcagat gccttcttcc cattttcgga taacgtggaa gaaatcgcca aagcaggaat
    22021 taaggccatc atccagcccg gtggctctgt ccgtgaccaa gaatccatcg aagcagcgaa
    22081 taaatacggc ttgactatgg tctttacagg tgtgagacat tttagacatt aagaagataa
    22141 aagggaagaa aacagtttct ttcctttttt ggcttaaaat gctaactgaa acaagattaa
    22201 aacgaacttt tttgatataa tgttggtaaa taattcgcaa aagaggttga ggaatgaaac
    22261 tgcttgttgt cggttctggt ggtcgtgagc atgcgattgc taaaaagtta cttgaatcaa
    22321 aagacgtgga aaaagtcttt gtagctcctg ggaatgatgg gatgactctg gatggtttgg
    22381 aattggtaaa tatctctatt tccgaacatt ctaaattgat tgacttcgca aagaccaatg
    22441 atgttgcttg gacctttatc ggtccagatg atgcccttgc tgctggtatc gtggatgatt
    22501 ttaaccaagc tgggcttaag gcctttggtc cgactagggc tgcagcggag ctggagtggt
    22561 ccaaggattt tgccaaggaa atcatggtca aatatggtgt ttcgacagca gcctatggca
    22621 cattttcaga tttcgaggaa gcaaaggcct atatcgaaaa gcatggtgcg cctatcgtag
    22681 tcaaggcgga tggcttggcg cttgggaagg gtgtcgtcgt tgctgagacg gttgagcaag
    22741 cggtcgaagc cgctcatgag atgcttttgg acaataaatt tggtgactca ggtgcgcgcg
    22801 tggttattga ggaattcctt gaaggagagg aattttcact ctttgccttt gtcaatggtg
    22861 ataagttcta catcatgcca acggctcagg accacaaacg tgcctacgat ggcgacaaag
    22921 ggcctaacac gggtggtatg ggtgcctatg cgccagttct acacttacca cagagtgtag
    22981 ttgatacagc ggttgacacc attgtcaagc cagttctaga agggatgatt aaagaaggtc
    23041 gcccttatct aggtgtcatt tacgcaggac ttatcttgac agcagacggg ccgaaggtca
    23101 tcgagttcaa ctctcgattt ggagatcctg aaactcagat tatattgcct cgtttgacat
    23161 ctgattttgc gcaaaatatc acggatattc tggatggcaa ggagccaaat atcatgtgga
    23221 cggacaaggg tgtgactctg ggtgtggttg tcgcatccaa gggctacccg ctagactatg
    23281 aaaggggcgt tgagttgcca gccaagacag aaggcgatgt catcacctac tatgcagggg
    23341 ctaagtttgc ggaaaatagc agagcactgc tctcaaacgg cggacgagtt tatatgctcg
    23401 ttaccacagc agataccgtc aaagaagccc aagccagcat ctaccaagaa ctatcccaac
    23461 aaaaaataga aggactcttc taccgaacag atatcggaag caaggcaatt aagtaaagat
    23521 ataagaataa cgcgccgtag tcgccaaaca cgataatggt cgtcgtggtg aaaagaccag
    23581 aacagtgaat gttctggtca gggggaaact tggagaccgt aggctcaaag tttaggaatg
    23641 aaaccgaagg tttgcttccg cctctatcac ctaagaccat tatcaaaaag aaaaataaaa
    23701 attcacaaaa tacgttaatg atcgtatggt ttgcgagcgt tagcgagcta atatagaaca
    23761 atcaccgccg ttgtgaaaga acgattggat gataatccaa tcgttcaggg aaattggaag
    23821 accttgggtt tccaatttag gcatgagaca cctttggtgg ctgctgccgt ccctcacaag
    23881 ctaaggtgat tgttgaaaaa gaggaaaaag gagaagaaat gaaaccagta atttccatca
    23941 tcatgggctc aaaatccgac tgggcaacca tgcaaaaaac agcagaagtc ctagaccgct
    24001 tcggtgtagc ctacgaaaag aaagttgttt ccgcacaccg cacaccagac ctcatgttca
    24061 aacatgcaga agaagcccgt agtcgtggca tcaagatcat catcgcaggt gctggtggcg
    24121 cagcgcattt gccaggcatg gtggctgcca aaacaaccct tccagtcatt ggtgtgccag
    24181 tcaagtctcg tgctcttagt ggagtggatt cactctattc tatcgttcag atgccgggtg
    24241 gggtgcctgt tgcgaccatg gctatcggtg aagctggagc gactaacgca gctctctttg
    24301 ccctccgtct cctctctgta gaagataagt ccattgcgga tgcacttgcc aactttgctg
    24361 aagaacaagg aaaaatcgca gaggagtcgt caaatgagct catctaaaac aatcggaatt
    24421 atcggtggcg gtcaactggg tcagatgatg gccatttctg ctatctacat gggccacaag
    24481 gttatcgcgc tggatcctgc ggcggattgc ccggtctctc gtgtggcgga aatcattgtg
    24541 gcaccttata acgatgtaga cgccctccgt cagttggcag accgttgcga tgtcctcact
    24601 tacgagtttg aaaatgtcga cgctgacggt ttggatgccg ttatcaagga tggacaactc
    24661 cctcaaggga cagacctgct ccgcatttcg caaaatcgta tttttgaaaa ggactttttg
    24721 tcaaacaagg ctcaagtcac cgttgcacct tacaaggttg tgacctcaag ccaagatttg
    24781 gcagatatcg acttgtcgaa aaactatgtt ctcaagactg cgactggtgg ctacgatggt
    24841 catggacaaa aggttattcg ttcagaagca gacttggaag cagcctatgc gctagcagac
    24901 tcagcagact gcgtcttgga agaatttgtc aactttgacc ttgagatttc tgtcatcgtg
    24961 tcaggaaatg gcaaggaggt gacgtttttc ccagttcagg aaaatatcca ccgcaacaat
    25021 atcctgtcta agaccatcgt accagcccgc atttctgaaa gtctagtaga caaggctaaa
    25081 gctatggcag tgcgaatcgc agaacaactc aacttgtctg gaactctctg tgtggaaatg
    25141 tttgcgacag ctgatgacgt tattgtcaat gaaatcgccc cacgaccaca taactctggg
    25201 cactattcta ttgaagcctg tgatttctct cagtttgaca cccatattct cggtgttctc
    25261 ggagcaccat taccagtcat caaactccat gcgccagccg ttatgctcaa tgtcctaggt
    25321 caacacgtcg aggctgctga aaagtatgtc gcagaaaatc caagcgccca cctccacatg
    25381 tatggtaaaa tagaagcaaa gcataatcgt aagatgggac atgtgacctt gtttagtgat
    25441 gtaccggata gtgtggatga gcttggggaa gggattgatt tttaggataa gtctatgata
    25501 caaattatcg ttaatacatt tattgaaaag gataagactg gagcagttgt tgaagtgttg
    25561 tatgccagtg ctgaccaaga taaggtacaa gctaaatatg aagaactagc tgcacaatac
    25621 cccgaaaatt atttagctat ctatgatgta ccgctggata cggatttgaa tacactagat
    25681 cattacccgt ctgtgtttat tggaaaagag gagtttgagt agaaatcttg gtttacctag
    25741 atagcttatt cccaacagct taagaagaaa ggaaaaatta acacatgatc aaccgttact
    25801 ctcgccctga gatggcgaat atttggagtg aagaaaataa ataccgtgct tggcttgagg
    25861 tggaaatcct ctctgacgag gcatgggctg agttggggga aatccctaag gaagatgtgg
    25921 ctttgattcg caagaaggcg gactttgaca tcgaccgtat tttggaaatt gagcaggaga
    25981 cgcgccacga tgtggtggct ttcacgcgtg cggtttctga gactcttggt gaagagcgca
    26041 agtgggttca ctatgggtta acttctactg acgtggtgga tactgcttat ggttacctct
    26101 acaagcaggc caacgacatc atccgtcgtg accttgaaaa cttcactaat atcatcgctg
    26161 acaaggccaa ggagcacaag ttcaccatca tgatggggcg tactcatggt gtgcacgctg
    26221 agccgacaac ctttggtctt aaattagcaa cttggtacag cgaaatgaaa cgcaatatcg
    26281 agcgcttcga gcatgcggct gctggtgtag aagctggtaa gatttctggt gcggttggga
    26341 actttgccaa tatcccacca tttgtagagg agtatgtctg cgataaactt ggcatccgtg
    26401 cccaagaaat ctctacacaa gtccttcctc gtgaccttca cgctgagtac tttgcggttc
    26461 ttgccagcat tgcgacttca atcgaacgta tggcgactga gattcgtggt ctacaaaaat
    26521 ctgagcaacg cgaagtagaa gagttctttg ctaaagggca aaaagggtct tcagcaatgc
    26581 ctcacaaacg caacccaatc ggttctgaaa atatgactgg tctggcgcgt gtcattcgtg
    26641 gtcacatgat tacggcttat gaaaacgtcg ctctctggca cgaacgcgat atttctcact
    26701 catcagctga gcgtatcatc acaccagata cgaccatttt gattgactac atgctcaacc
    26761 gttttggaaa tatcgtcaag aacttgacag tcttcccaga aaatatgatc cgaaacatga
    26821 actcgacttt tggtcttatc tttagccaac gggctatgtt gacattgatt gaaaaaggca
    26881 tgacccgtga gcaagcctat gacttggtgc aaccaaaaac agcctactct tgggacaacc
    26941 aagtagactt taaaccactt cttgaggcag attcagaagt aacatcacgt ctcactcaag
    27001 aagaaatcga tgaaatcttc aacccagttt attacaccaa acgagtggat gatatctttg
    27061 aacgtcttgg actaggtgat taattaa
//
LOCUS       DAVHIT010000024        24002 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVHIT010000024 DAVHIT010000000
VERSION     DAVHIT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.024"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..919)
                     /locus_tag="VVN16_000848"
     CDS             complement(<1..919)
                     /locus_tag="VVN16_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5572269.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKVLLEGCSQRSVSLDYALPNQGMLSFWLAQYKKN
                     GYTIVEKTRERVPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKSIK
                     LARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRV
                     QGLIKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPA
                     STQKLYLSPVLDGFNSEIIAFNLSTSPNLE"
     gene            complement(1292..3262)
                     /gene="tkt"
                     /locus_tag="VVN16_000849"
     CDS             complement(1292..3262)
                     /gene="tkt"
                     /locus_tag="VVN16_000849"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5572270.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKFP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            complement(3299..3994)
                     /locus_tag="VVN16_000850"
     CDS             complement(3299..3994)
                     /locus_tag="VVN16_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5572271.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            complement(4005..5090)
                     /locus_tag="VVN16_000851"
     CDS             complement(4005..5090)
                     /locus_tag="VVN16_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEU5572272.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            complement(5116..5427)
                     /locus_tag="VVN16_000852"
     CDS             complement(5116..5427)
                     /locus_tag="VVN16_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU5572273.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            complement(5455..5904)
                     /locus_tag="VVN16_000853"
     CDS             complement(5455..5904)
                     /locus_tag="VVN16_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5572274.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            complement(5882..6310)
                     /locus_tag="VVN16_000854"
     CDS             complement(5882..6310)
                     /locus_tag="VVN16_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5572275.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            complement(6303..7766)
                     /locus_tag="VVN16_000855"
     CDS             complement(6303..7766)
                     /locus_tag="VVN16_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU5572276.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            complement(8295..10631)
                     /locus_tag="VVN16_000856"
     CDS             complement(8295..10631)
                     /locus_tag="VVN16_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5572277.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            10764..11513
                     /locus_tag="VVN16_000857"
     CDS             10764..11513
                     /locus_tag="VVN16_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5572278.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(11806..12420)
                     /locus_tag="VVN16_000858"
     CDS             complement(11806..12420)
                     /locus_tag="VVN16_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU5572279.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(13236..13505)
                     /gene="rpsO"
                     /locus_tag="VVN16_000859"
     CDS             complement(13236..13505)
                     /gene="rpsO"
                     /locus_tag="VVN16_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU5572280.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(13646..14275)
                     /locus_tag="VVN16_000860"
     CDS             complement(13646..14275)
                     /locus_tag="VVN16_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5572281.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     FYIDETKTTRLLDTSDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKECISLLEK"
     gene            14513..14713
                     /locus_tag="VVN16_000861"
     CDS             14513..14713
                     /locus_tag="VVN16_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572282.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            15143..15283
                     /locus_tag="VVN16_000862"
     CDS             15143..15283
                     /locus_tag="VVN16_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU5572283.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            15290..15466
                     /locus_tag="VVN16_000863"
     CDS             15290..15466
                     /locus_tag="VVN16_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU5572284.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            15524..17467
                     /gene="thrS"
                     /locus_tag="VVN16_000864"
     CDS             15524..17467
                     /gene="thrS"
                     /locus_tag="VVN16_000864"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5572285.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(17516..18490)
                     /locus_tag="VVN16_000865"
     CDS             complement(17516..18490)
                     /locus_tag="VVN16_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU5572286.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(18483..19160)
                     /locus_tag="VVN16_000866"
     CDS             complement(18483..19160)
                     /locus_tag="VVN16_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572287.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            19316..20389
                     /locus_tag="VVN16_000867"
     CDS             19316..20389
                     /locus_tag="VVN16_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU5572288.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(20480..20782)
                     /locus_tag="VVN16_000868"
     CDS             complement(20480..20782)
                     /locus_tag="VVN16_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572289.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPNYLSMLFDFY"
     gene            complement(20850..21287)
                     /locus_tag="VVN16_000869"
     CDS             complement(20850..21287)
                     /locus_tag="VVN16_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5572290.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(21363..22205)
                     /locus_tag="VVN16_000870"
     CDS             complement(21363..22205)
                     /locus_tag="VVN16_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5572291.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHIGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            22317..22967
                     /locus_tag="VVN16_000871"
     CDS             22317..22967
                     /locus_tag="VVN16_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU5572292.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            22948..23139
                     /locus_tag="VVN16_000872"
     CDS             22948..23139
                     /locus_tag="VVN16_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572293.1"
                     /translation="MSRKSTNFSSPLPTLKVLFSGFYFYHFILLSFGGRIFEKNGRVF
                     EKKKGSLTYEKITKNFCYI"
     gene            23165..23811
                     /locus_tag="VVN16_000873"
                     /pseudo
     CDS             23165..23811
                     /locus_tag="VVN16_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            23942..>24002
                     /locus_tag="VVN16_000874"
     CDS             23942..>24002
                     /locus_tag="VVN16_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HEU5572294.1"
                     /translation="MKKLYRIHFIAIAVIDLLLF"
BASE COUNT         7335 a         5339 c         4318 g         7010 t
ORIGIN      
        1 gttctaagtt gggcgaagtt gaaagattaa aagcaataat ttcgctgtta aagccatcta
       61 aaactggtga taagtaaagc ttttgagtac ttgctggaat ggcaaattct gtcacatctg
      121 tgtagcactt ttccattgtt ttagagcctt caaattggcc ttgaatgaga ttctctgcct
      181 tcttgccaac gtctccttta tgagaagaat attttcgttt ctgtcgcatt ttagcttgta
      241 aattgagtac ttttatcaag ccttgaactc ttttatgatt taccagataa gcacgatttc
      301 ttagttctaa atgaacccgg cgataagcat aatttccttt gtgttcgata aaaatggatt
      361 gaatttcagc tttaagctct tggtccttat ctgttttgtc tagctgtttc aagtgatagt
      421 agtaggtcca acgagctagt ttaatggatt ttagaagaag aactaacgaa aactcagtca
      481 ttaattcttg aacaatttct gtctttcttc tttctctttt tcctccttca atcggagttc
      541 tcttaacttt tttaggatgg cattctccgc tctcaggtac tctctctctt gttttctcaa
      601 caatagtata cccgtttttc ttgtattgtg ccagccaaaa tgaaagcatt ccttggtttg
      661 ggagggcata atcaagacta acacttcttt gtgaacaacc ttccagtaag actttatcca
      721 tcatttcttg tttcaattta gaagaatagt gacgattctt ccccttttta acgatttcta
      781 tgccataacg gtcaattaat ttcaccatgt actttagacc agaaacatcc acaccaaatc
      841 tttttgaaag ctgtttgaag ctttgtcctt gctttcttag ttcatagatc tgaactttat
      901 cttcataact caatttcata ataaaacacc ccaaaagtta gatttattct gtctaacttt
      961 tggggtgcag ttcatcattt caacatccta tactatgcgt tttttaattt tagagacctt
     1021 ctctctagct ttcaaaaaac ttacaatatc ctaagtcaat ctgtggatgc tgtgtttatt
     1081 gattaaagaa actatctaga tcagtagcaa agtgattagc gtacttcgca accgtttcag
     1141 agtatactct attctgtata ctatctacta taactttcgt agataaatta ttacattctc
     1201 attatagttt ttaaataacc aatcatctga ttagtgtcta tcactacatc tgctagtctg
     1261 acaacagctg tatcttcatt gtaattggtt tctataggga cttaacttga gcaacgatat
     1321 tctctaccgt aaatccataa ttatcaatca cagtctgagc tggggcagac gcaccgaaga
     1381 tgtcaatacc gatgaccgcg ccatccaaac caacatactt gtaccaactt tgggtcgctg
     1441 ccatttcaat ggccacacga cgacgagtct tagatggtaa aatgtcttcc ttgtaggtag
     1501 catcttgagc atcaaatagt tcggttgagg gcatagatac cacacgtact ttaccacctt
     1561 gtaaaaccaa ttccttagca gctttgatag ctagattgac ctcagatcct gtagcaatga
     1621 taatagtatc aaatcccggg gtatcataca cgacgtaggc tcctttagcg accttaccaa
     1681 agtctgtccc ttcttcaact accaagtttt gacgggttaa gacaatgaca gttggagtgg
     1741 tggtactggt caaggcatga tgccaagccg cttgagtttc acgggcatcc gctggacgga
     1801 taacagtcaa gtttggcatt gagcgtaaac ctgccaaatg ttcaactggt tcatgagttg
     1861 gaccatcttc accaacggca attgaatcat gggtaaagac ataagttaca ggcaactcct
     1921 gaatggctga tagccgaata gcagctttga cgtagtcaga gaaaacaaag aaggttccgc
     1981 cataaactcg taaaccacca tgaagagcca ttccattgag gattgttccc atggcaaatt
     2041 cacgtacccc aaactgaata ttgcggttta atggattata tttatcttgt aagccatctg
     2101 ccttgatgta ggtcatgttg gagtgagcta agtctgccga tccacctaag aaggttggta
     2161 aaactgctgc tgctgtatta atagcatctt gggacgaatt acgagttgct tgagagaagc
     2221 cattctcata gacagggaag tctttttcag taatggttac agggaatttt ccagctacaa
     2281 tagcgtcaat ctcactagca acttcgggat aagcaacctt gtaatcagac accaaactag
     2341 cccaagcatc gtatgcctcc tgaccacgat ccgctacatt tgtcttgaaa tcagaatata
     2401 cttcctctgg tacttcaaat ggatcgtaat cccatcccaa aaacttacga gttgctcctg
     2461 tttcttctgc tcctagtggt gcaccatgaa cagcatttgt accactttta ttgggtgagc
     2521 cgtaaccaat taccgttttc acttcaatca aactcggctt accagaaaac ttggccgtct
     2581 caattgctgt agaaattgct gctaaatctg ttccatcttc taccagaact gtatgccaac
     2641 cataagcatc gtaacgggcg cgaacatttt cagagaaagt atctttggtc tcaccatcca
     2701 agcagatgtc gttggagtcg tagaggacga taagcttatc taaagcttga tgacctgcat
     2761 aagaagccgc ctccgcagac actccttcca tgaagtcacc gtctccagca ataacataag
     2821 tataatggtc aaaaataggg aaaccatctt tgttgtactt agcagctaaa aaacgctctg
     2881 cttgggcgaa accaacggca gtagaaattc cctgaccaag cggaccagat gtcgcatcca
     2941 caccagacgt atgcgtcact tcaggatgac caggtgtctt agatccccat tgccggaaat
     3001 ttttaatctc gtccatggat acatcgttat accctgttaa atgcaagaga gcatacagta
     3061 gcattgatcc atgacccgca gacaagataa agcgatctcg gttaatccag tttggctgct
     3121 caggtgtaat tctaaggtgc tttgtaaata ggctataagc cattggcgca gcacccatga
     3181 cgattcccgg gtgaccagaa ttagatttgt taatagcatc tacgccaaga aaacgaatgg
     3241 cgttaactga ttgtagtgac atatattcct cctaactaat aaatttcttc tcaaaccatt
     3301 atttgattgg cattgttttt ccggtcattt cttcgtaatc tctagaacag atatcccagg
     3361 ctttggcaat atcgtcatcc aaaccaaata atccactacg acctataaca taaatatctg
     3421 gtcctgccac atcaatttgt ttgaaagtct tacgactcga agaaccatcc atctcaatga
     3481 tgtagtgata accattctga actctaagct gacggagttc ttggatttta tacaaggtag
     3541 actccaaaaa gcgttgtcct gcaaaacctg gatctacagt cataatagtt actttgtcaa
     3601 gtaaatcaat gtagggaaag attgtagaaa caggtgtttc aggattaagg acaacaccag
     3661 cctttagacc tgcatcatga attttatcaa tcaaacgaaa agcaagacca ttcagaactt
     3721 cagcatgaat acaaatatac tcacattgta aatcgagaac ttgatctacc caaaaggttg
     3781 ggtctgtgac catcagatga actgataaag gtgtgtcact aattttttga acttcttgaa
     3841 tgaaccaagg agacaaggta atattgggaa caaaatggcc atccataata tcgatatgat
     3901 aagatgctac tttatcattc aaaaaagtaa tctgctcttt gaatttgtcc aaatccatgg
     3961 tcatcaaaga tggtgaaaat tcaattcttg acatcgttac cctcttacaa aatttcaata
     4021 tctgacaaat caatctcttc ttcaacagtc ataactggtt ctgtatgttt tattggtttt
     4081 ttcaaaatcg cgtagacaag tcctgtgact acaatgttgg ctaacaaagc acaaatacca
     4141 gctactggac gagtcatggt tggtaacata agcactccac caaatggcac ggcagaatca
     4201 gcacccattg tcaaagaaac agcaccacca acagcaccac cgatacctgt tgcaatgaga
     4261 cctggaacca agttattcat aacaatcgga attacacctt caacaatatt gacaatcccc
     4321 ataggaacag ccgatttcaa tgtttcgatt tcctcttgag tatagatatt ttttttgagt
     4381 aatttcgcga taaaataggc caatccaaat ccaactggtg tagcagtatt caccaattgc
     4441 aaagcagtca atggttcttt cacaccttca gcctgtaaag tcaacacaaa cgcatagact
     4501 gttttattaa gtgggccacc aaagtcaaca gcactgagaa ctccaataac tgcccccatc
     4561 aaaccatttg aagcacttcc caagctttgt aataagctcg tcaaccagtt ggtaaaggct
     4621 gcgataggcg ctccaataat ataaatcata atcaaactac ttaccaaaga ggctacaaaa
     4681 ggaataatca aggttggcat taaaccttta atccagtttg gtactttgac ctttttaata
     4741 atcgcttgaa ccaagaaacc agctatataa cctcccaaga taccaccgat aaaccctgaa
     4801 ccaacagaat tggcaattag accaacaaca aaacctggtg caatccctgg tttaccagca
     4861 atcgagtaag acaaacctgt agcaataaca actggcaaga gaccaagggc tttaccaccc
     4921 atagttgcta aagcatccca gatagtgaat tttcctgcta caagagcgtc aggaacacca
     4981 ccccccattg ctaaaccaat ggcaactaag aatcctgcac cacaaacaat tggaatcata
     5041 taggaaatgg ctgtcaataa gtgacctttt aagtttaagt gtttcaacat atattttctc
     5101 cttaaatatt ttcagttatt tcgtaacaat ctcaacagct ttagcaatca gtttattggg
     5161 agatttgact gccacttctg ttggaacctt gataatcttt ttaccctcaa agcgttccat
     5221 accagaaatc ttaacatcaa ctgctaaaat aactacatcc gctgcatcaa tctgctcttg
     5281 actcaattca ttttctaccc ctattgtccc ctgagtctca acatgaatca catgtccagc
     5341 tacctttgcg gcattctcta atttttcctg tgcaatataa gtgtgggcaa ttcccacagt
     5401 acaagctgca acaccaacaa ttttcatacg gataccctcc aaaatttttt cttattaaca
     5461 aaaagctgca atcacatcat cagatgtctg agcccgaact aatttggcaa caacttcgtc
     5521 attaccaagt tttcgagcaa agagtgataa ggtcttcaaa tgctccctag cagcttctgt
     5581 atcatcacca actgcaaaga gtacaattac tttgacccct ttcccatcaa tggtctccca
     5641 aggaatctca ttgtgattta tagctatgac tacccccgcc ttctccacag cagaactctt
     5701 gctatgggga atagcaatat aattcccaat accggtctgt ccttctgcct ctctctgata
     5761 aagaccttcg ataaattggt ctctatcaga cacataaccc gtctcaacca atagttgagc
     5821 taatgcctca aaaacctctt ctttgctctg catctgtaaa tccgtctgga tcagactcac
     5881 attaagaata tctttgattt ccatatatta tctcccgtaa ttcttctttt gttaactgtt
     5941 ttaattgatt tatgaatgat tcatctgcta gtcttctcat caatgtttta atacatgact
     6001 tgtcctgtga tactgcaatg gccaaaccga taataaggtc aacacactgg atatccttcg
     6061 accattctct gataggtgat tttaatctag taatcactaa gacatgatgt tgaaagtttc
     6121 cttcacaatg tggtagaaga acacctttag caacctctat acttccctgt ctctcacggt
     6181 aatatagaag ctcttctatt ttttctgtat cttcagaaac aagaaggctg atttgatttg
     6241 ctaattcttt gtaggcttct tgacgatttt gaacagatat atccataagg acaagcgaaa
     6301 gattattcat agtttatctc ctgaattttt gcttgaagac gttgtttatc accctcggtt
     6361 agaaaaacac taactaggac aaacgggaca cttgctggtt cctgcaaagc taccgtcgtc
     6421 acaatgaaat ctaaatctgg atatagattt atcagctcat ctaattgatg ataagcaact
     6481 acatcaataa tatccaattc agtaaattgc ttttctaatc gtgctcgtaa aagctctgaa
     6541 gttccgacac ctgatgtaca catcactact gtttttagag gacgtgctcg cttttcttga
     6601 aaccgtgcaa aatataagac tagaaaacca atctcgtcca gactcaagga cgcaaaacca
     6661 aatactagac tcacttcttt agaaatagtt gttaactctt taaccagatt aggataggtt
     6721 aaaagaattt gtgataaaag actattctta atctgtactc tattatctaa tctcctcagt
     6781 aagggactga tatgactcgc caagtcaaca aagtcaggat ctgtcgtttc aatctctcta
     6841 ttgtccatac acatacggct aaaatagtaa tgagtgacat ctaaaactcg ctgagaaaaa
     6901 ggaagcttcc cggaagaaaa tggtttttgc aatctcgaag atacaacgta ttgataaaga
     6961 tagtcaatct caactgcatc aaccttcatc ttaaaatatt gttcaatttc ttgaataatt
     7021 ttttgacaga cactgtaaat tttctcatca acaatagtag gttttgaagg tgctacaaca
     7081 tgaatactag tactacgacg attcctactg ataaaaatat acaggtgaga gaaaatattt
     7141 atattatatg gatagggaat gtttaccccc aattctctct caagtgtaat cagtaatccc
     7201 aaacaaaagt gagcaagttc tccgtcaaga ggctgaacct tattctgtgt aatttgctct
     7261 aaatccaact gattaaacat tggcagtaga tttagaatgg ctgaacgaat ttgagcctca
     7321 tccccatcaa taaaaagctt tcgttgtctc atttttaaat ctaacccata aattgctaga
     7381 ctctcttgta atatctgacg atcttttagt actactgact cgcttacgta gaattcctct
     7441 cctaaatcat aaatagaatg tggcttagga gcagtcaaca agagacgttc tagtaatttt
     7501 tcacgcctta cttcaggatc aaaagcctct gtaaaattcc catcaacgtc cacgagggaa
     7561 cttcttggat ttaatttgaa gcctctgcct ttttcagata aaatgaatgc ttctaccgga
     7621 caatctttgt tgattacctt gacaaggcga taaaccgttt tatcagagca atttaatttt
     7681 tctcccaata ctttagcaga tatataatcc ttactatctc gcaaaaggag caacaacgtt
     7741 tccatttgtt cttgttttct agacacgata cacctccttt atgattccat tatatcgctt
     7801 tcaaaaaata atatacttcg cattttgtcc tttgcaatgg acatttttgt ttttatcaaa
     7861 aatatgatat caaatagaac agaacctcac tatgccaaaa tcattttacc ttttcttcat
     7921 aacatctatt ttagttgaaa tatttaagtt gcttttcgta tttgataaga aataaaccaa
     7981 acgattttct aataaataac actatgttcc atttaacaat agatagcatc ttcattttca
     8041 aactaccaac ctatccgacc atttgttcta gcaatagaag agagttaaaa ttacttcaat
     8101 ctgtcgcttt ctctttctta catcaaactt caaatagtct aactacagat atttgaatct
     8161 attacctcaa tccaagcagg cttgagaatc actcttgggt tcaccgatag ataatagaca
     8221 tgtaggcctt gcacatcaga tctacgatag atccatcgta taaaaaactg caatccacag
     8281 attgcagtct ttctttattt tttcgcttgg taacgactgg tcaggatgaa aatcacggta
     8341 aagaccaaca tcatgacacc ataaacaggt aatgtttgtc ctgttaaggt tgaaatttca
     8401 agcaattttt ggattcttgg gaagagagct gtggctagga agcctcctac tgaccagaca
     8461 atcaagagga cacgccatag ggtgaatggc atgcaggctc taaatacgga taagaaacca
     8521 attgacccca agagataata gaggagagtt gagatttcta actcagacca accttggcta
     8581 ctcccaaata ttttcacaaa caggacgctg aagacgacca tgagagcgct tggtagggca
     8641 cgaagcatgg attttctgag gaaatttggc tcaacaggtt tgatatttcg ctcaaaagtc
     8701 agaacgaatg gtgggaaacc ttccacaaac tggtcaatca tggtaatctg gattggaatg
     8761 aaggggaaaa tcaagatcca ttcagaccga cctagtaaag cactggcgat acagataact
     8821 gcgagcaaga atgagtagat ggtctttatc aagaaaatcg gggcgatgtg ggcaatgtta
     8881 ttgaccacgc gacgaccctc gaagagaatc tcaggaacat cattaaagtc tgagttcaag
     8941 agaaccagat tggcaatctg acgggttgct ggatctccct ccgccatcac gatagaacaa
     9001 tccgcctcac gaagggccaa gatatcatta accccgtccc ctgtcatagc cgttgtatga
     9061 cccgcttttt tcaacgtttg gatgatgagt ttcttttgat gaggggaaac acgtccgaaa
     9121 atagctgtct cctccgccat ggccatcaat tcctcatcgg tgatttttga gcaatctaca
     9181 tagctgtgat agtccgcaaa accagccttc tgggcaatgc tggacaccgt aactggattg
     9241 tcaccagaga taatcttgag tcccacctct tgagaacgga gatagtccag cgtctctgct
     9301 gctccctctc gaatggggtc caagatttcc agcaaggcta gagcctgaat atcagatggt
     9361 ttctgtggtt tgtgatggtc taatttctcc tgactgagag ctaagaccaa gacacgtgaa
     9421 cctctctcca aggcctctct ggcttctggg acctcagaat ccagcaacat ctcaggtgcc
     9481 cctaagaaaa ctgtccccaa gccttctaac tccatagccc cccacttgcg gtcgctcgag
     9541 aagggaagat tggaaatcat aggataagca acatctccca caaaacgctg gcgaatggct
     9601 tgggcagttg gattcttatc ctcactatgg gccatgtagc tggtcaagat gctggcaata
     9661 gcctcttcac catacgtttc cgtcaacgga agaacagcct ccacctgcat ctttccttgg
     9721 gtgatggtac ccgtcttgtc cagacagagc atatccacgc gcgccaaggt ctcaacagag
     9781 tacatctcct gcaccaagac ctttttcaag cccaacttaa tcactgcagt caagagcgaa
     9841 gtaatggtca aaagggcaat tcccttaggc aacattccca aaagagctgt cgacgagttt
     9901 acaacggatg acttgagagg caggcctttt aaaagcaagg cttccagcaa gagagccaga
     9961 ccaaagggaa tgataatctt cccagtaaaa ccagccaact tgtccagcga tttcatgata
    10021 cgggagttga tgggtttaac ggtcttggct tccagcataa gtttggcagc atagttgtct
    10081 gcaccgacat ggtgaacttg agataaaact gacccactgg ctaggaaact tcctgacaaa
    10141 agtaagccgt caacttcctt ttgcaccaaa tcactttctc ccgttaacat ggcttcattg
    10201 acttccgcaa agccttccaa aaccaaggca tcactaggaa tctgctctcc tgcagacaaa
    10261 cgaatgacat ctcctagcac taattcttca ggattaagag caacttcctg accatcacgg
    10321 atggttttga ccttttcctt ggtcatgaga ttgagcttgt ccaccatgtg tttggctcgt
    10381 agctcggtca caatcccaga aaaagcgtta aagcagataa cagcaaagaa gaccagattg
    10441 ctccaagcct gcacaaaggc aagggccaaa gcaatggcaa agttcaaagc gttaaaaagg
    10501 gtaaagacat ttcgtttaac gatttgccaa gtgctggtac tggctgatgc ggtaaagtca
    10561 ttgaccaaac cctcagcctg tctttccttg acttctcttt gggttaatcc cataatctta
    10621 tttttatcca taaattctat tatatcattt tttttaacag aattctaatt tcatcccaaa
    10681 tatgcatcta gtcaagggaa aagtttgcca gccctccttt gataaggtat aataataaca
    10741 agaaaacaat cgaaggagat cgcatgtttt atacttattt gcgtggatta gttgtattgc
    10801 tcttatggtc catcaatggc aatgctcact atcataatac tgataaaatt cctaatcaag
    10861 atgaaaatta tattttagtt gcgcctcacc gtacctggtg ggatcctgtt tatatggcct
    10921 ttgcgaccaa gccaaaacag ttcatcttta tggcaaaaaa agaactcttt accaaccgta
    10981 tctttggttg gtggattcgt atgtgtggcg cctttcccat cgaccgtgaa aatcccagcg
    11041 cctcagccat caaatatcct atcaacgttc tcaaaaaaag tgaccgctct ctcatcatgt
    11101 ttccaagtgg tagccgccac tcaaacgatg tcaagggggg cgcagcactg attgccaaaa
    11161 tggccaaggt ccgtatcatg ccggttacct acaccggtcc catgactttg aagggcttga
    11221 ttagccgtga acgtgtcgat atgaactttg gaaatccaat cgatatctca gatatcaaga
    11281 aaatgaatga tgaaggcatt gaaacagtcg ccaatcgtat tcaaacagaa ttccaacgtc
    11341 tggacgaaga aacgaaacaa tggcacaatg ataaaaaacc aaatccactc tggtggttta
    11401 tccgcatccc tgccctcatc cttgctatta tcctcgctat cctaaccatc atctttagct
    11461 ttatcgcaag cttcatctgg aacccagata agaaaagaga agaacttgca tagaagaaat
    11521 gaaccttggc caaacagcta aggttttcat ttatatatca aattgaaact agaatagtac
    11581 acctctactt cttaaaacat ttttagaaat cgatttgact gtcctgatcg atttgtcctg
    11641 tttttgtttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
    11701 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccctct gaaaatcaga
    11761 gagctgattt gagctcgggc taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
    11821 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
    11881 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aggataacac
    11941 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
    12001 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
    12061 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
    12121 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
    12181 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
    12241 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
    12301 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
    12361 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
    12421 aaaaaaaacc tcctgtatta gtcagtaata aatcagcaga ttttaggaga gcacagacac
    12481 attaatttag ctatcgctag tgggtaatgt cgaacgtagg aaagcatttt actcctcctc
    12541 aaaacgtagt tacaaagtaa tttctaactg gaattcagtg actacttcca tgtaaagtat
    12601 aacatactat actttacttt gtaaagagtg ggctcttcta gggggcttgc atacagttac
    12661 tcggcttgaa aagccgagta actgcgcacc tttatggtgt aattagctga taccatttga
    12721 ggtttttgta gtctccaaaa ttgttaccga taaaacctca ataaaaaagc cctctgaagt
    12781 cagagagctg attttagccc gggctaaaat cctaatgaaa aagatgaaac tccttgtgct
    12841 catcgaacac tgtgtcattt ccctattttc atacggattt ttgacgccct tagcatcatg
    12901 attcttgcta gataaatcgt ttatagacta ggcgacgagg cgaagatcgt acgaaacttt
    12961 cagtgaaaca aaaaaaatct ccccgaaggg agaaaagcta gtttatttta ccttacagtg
    13021 ctaggaaccg taaccgaaga ttatacttga gtatagcgag gtaaggtgac aacgcaaaaa
    13081 agctacccaa tttagagtag ctttattatc aagatatgct caattgaaca cggcctaagc
    13141 acttggcaaa aaagataaaa tctcctagaa actgaagttt cttcgtcaat tttcctattt
    13201 ttgctttgtg cttttgacag cctttgtatc ttgaattagc gacgaagtcc tagagagttg
    13261 atcaactcac ggtaacggtt aacgtcgttt ttacgcaagt atgcaagcaa gttacgacgg
    13321 cgaccgattt ttttcatcaa tccacggtaa gtagcgtggt cttttttgtg ttgtttgatg
    13381 tgttcattaa ggtggttgat ttcccaagta aggacagcaa cttgaacctc tactgaacct
    13441 gtatcacctt cgtgacgtgc atattgtgca atgatttcat tttttttctc ttttgagatt
    13501 gccatgatgt ttctcctttt tatttggctt catccgagtg acaggttggc atgcctgtaa
    13561 ccaagaagaa gttatttgtc tttacgacaa tccctatttt accaagattt aaattttctg
    13621 tcaaacatta atactctcat agttattatt tttcaagtaa agagatacat tcttttatat
    13681 gatgaccttt ttcaagttca tctaccaatc caatagcata atctgcataa ctaatctggc
    13741 ttattccttt ttcattagta gtaaaaattt ctcctgctag aatgtagttt cctgtttttt
    13801 caccatcggg aataaaatct acagcaggac tgacaaaagt ccagttgaga ttattttttg
    13861 tacgaagaag atccaattca tccgcttgag cttttgctag tggcttaaat tcttcaggaa
    13921 aatcagacgt atctaaaagt cgtgtcgttt ttgtttcatc tatataaaag cttcctgatc
    13981 cccctacaac tagaaaacgt gtctgtgttc cagcaagaag ctgattaaat agttcgattg
    14041 atttgctgtg gagcggtagc gtatctggtg tataagcacc aaacgctgaa ataacagcat
    14101 caaatccagt aagatcatct tttgtcaact caaataaatc ttttttaata atagactcag
    14161 cttgactttt gttttcagaa cgaacaatag ccgttacttc atgtcctcgt ttgactgctt
    14221 cttcaacaat tgccttcccc gcttgtccat ttgctgcaat aactgctagt ttcatttttt
    14281 atacctctct tgttgtaatt attttagtta caacaattgt gacactctta ataatcaatg
    14341 tcaatagtct tgcttaatta ttatcaaaat atttctacca agaaaactaa ctatgattct
    14401 agtgaaaaga aaaatcttct ttgtcaacaa atttactttc ttgttttaaa catgctataa
    14461 taatcatagc aagagatcta agttgtctgt tttttaaaac gaggtgatta tcatgcgtag
    14521 attctattcc catctcccct actatctggt catattattc ttttattggc cactttatga
    14581 gttgttctta ctagttgttt ctgaccccct tacactcaag gaactctata taaacaatct
    14641 tctcttcttt acacctctgg taatcttgat tgtatcgtta ctctatagct accgtttccg
    14701 tttctcactt tgatggttag ttggtaacgg actgctcttt tactttacta tcataacctt
    14761 tggtgagttt atactaattt acttgctaat ctatgaaaca gttgctctgg tcggcatgga
    14821 ttctggtatt agcatcaagc atattctaca aaaaatgaaa aacaaaaact ttcacaaaat
    14881 ccttgaaaaa tctcacaatc atgctataat aatccataga gacaagtcac ttagtccctt
    14941 tctactagag agtgcgtggt tgctggaaac gcataggaag tctaaactga tactactctt
    15001 gagtttttta tgaaaacata aaacggtggc cacgttagag ccgatcagag gtgtccctct
    15061 cttttgaggt acataaatga aggtggaacc acgttgcgac gtcctttcga ggatgtcgca
    15121 tttttttatt aggatactaa ttatggagtt gcaagaatta gtggagcgca gttgggcaat
    15181 ccgacaagct tatcacgaac tggaagttaa gcatcatgat ttcaagtgga cggtagaaga
    15241 agacctcttg gctttatcta atgatattgg aaatttccaa tgactggtga tgacaaagca
    15301 aggacgctac tatgatgaaa caccctacac actggaacaa aaactttcag aaaatatctg
    15361 gtggctatta gaactttctc aacgtttgga tatagacatt ctgacggaaa tggaaaactt
    15421 cctctctgat aaagaaaagc aattgaacgt taggactcgg aagtagtctg ctgataaaaa
    15481 atcaatgctt agaaactatg aaataataaa aaaggagaac atcatgatta acattacttt
    15541 cccagatggc gctgttcgtg aattcgaatc tggcgtaaca acttttgaaa ttgcccaatc
    15601 tatcagcaat tccctagcta aaaaagcctt ggctggtaaa ttcaacggca aactcatcga
    15661 cactactcgc gctatcactg aagatggaag catcgaaatt gtgacacctg atcacgaaga
    15721 tgcccttcca atcttgcgtc actcagcagc tcacttgttc gcccaagcag ctcgtcgtct
    15781 tttcccagac attcacttgg gagttggtcc agccatcgaa gatggtttct actacgatac
    15841 tgacaacaca gctggtcaaa tctctaacga agaccttcct cgtatcgaag aagaaatgca
    15901 aaaaatcgtc aaagaaaact tcccatctat tcgtgaagaa gtgactaaag acgaggcacg
    15961 tgaaatcttc aaaaatgacc cttacaagtt ggaattgatt gaagaacact cagaagacga
    16021 aggcggtttg actatctatc gtcagggtga atatgtagac ctctgccgtg gacctcacgt
    16081 tccatcaaca ggtcgtatcc aaatcttcca ccttctccat gtagctggtg cgtactggcg
    16141 tggaaacagc gacaacgcta tgatgcaacg tatctacggt acagcttggt ttgacaagaa
    16201 agacttgaaa aactaccttc aaatgcgtga agaagctaag gaacgtgacc accgtaaact
    16261 tggtaaagag cttgacctct ttatgatttc acaagaagtg ggacaaggtt tgccattctg
    16321 gttgccaaat ggtgcgacta tccgtcgtga attggaacgc tacatcgtaa acaaagagtt
    16381 ggcttctggc taccaacacg tctacactcc accacttgct tctgttgagc tttacaagac
    16441 ttctggtcac tgggatcatt accaagaaga catgttccca accatggaca tgggtgacgg
    16501 ggaagaattt gtccttcgtc caatgaactg tccgcaccac atccaagttt ttaaacacca
    16561 tgttcactct taccgtgaat tgccaatccg tatcgctgaa atcggtatga tgcaccgtta
    16621 cgaaaaatct ggtgccctca ctggccttca acgtgtacgt gaaatgtcac tcaacgacgg
    16681 tcacctattc gttactccag aacaaatcca agaagaattc caacgtgccc ttcagttgat
    16741 tatcgatgtt tatgaagact tcaacttgac tgactaccgc ttccgcctct ctcttcgtga
    16801 ccctcaagat actcataagt actttgataa cgatgagatg tgggaaaatg cccaaaccat
    16861 gcttcgtgca gctcttgatg aaatgggcgt ggactacttt gaagccgaag gtgaagcagc
    16921 cttctacgga ccaaaattgg atatccagat taaaactgcc cttggaaaag aagaaaccct
    16981 ttctactatc caacttgatt tcttgttgcc agaacgcttc gacctcaaat acatcggagc
    17041 tgatggcgaa gatcaccgtc cagtcatgat ccaccgtggg gttatctcaa ctatggaacg
    17101 cttcacagct atcttgattg agaactacaa gggagccttc ccaacatggc tggcaccaca
    17161 ccaagtaacc ctcatcccag tatctaacga aaaacacgtg gactacgctt gggaagtggc
    17221 caagaaactc cgtgaccgcg gtgtccgtgc agacgtagat gagcgcaatg aaaaaatgca
    17281 gttcaagatc cgtgcttcac aaaccagcaa gattccttac caattaattg ttggagacaa
    17341 agaaatggaa gacgaaacag tcaacgttcg tcgctacggc caaaaagaaa cacaaactgt
    17401 ctcagttgat aattttgttc aagctatcct agctgatatc gccaacaaat cacgcgttga
    17461 gaaataagag tctagcataa aagcctccaa tctggaggct ttttctcatc tatttttact
    17521 caaggactaa gttcacttga gcaaactgaa tccgcactgt cgttcctttt ccgacctcag
    17581 actcgatacg aatctggtgc cccagttctt cagaaatttt cttagataga taaaggccaa
    17641 gtccagagga ctgctgggtc aaacggccat tgtatcctga aaagccacgt tcaaatactc
    17701 ggaggacatc actgtttttt atcccgattc ccgtatcttt gatacaaagc tcttggtcat
    17761 ccatataaat ctccagacca ccttccttgg tgtacttgag actgtttgag atgatttgct
    17821 caataaccac tagcagccac tttttatccg tcacgatttc tttatcaagg tcatgtagat
    17881 tgacatttaa gcctttttga ataaagaaaa gagcatattt acgaattatt tccttgacca
    17941 agtcctcaat ttgaacctgc tttaagagca aatcatcatg gaaactttct aaacgcaggt
    18001 actgtaaaac taggttggta taggagtcga ttttgaaaat ttcctgttct agctgctgct
    18061 tcagttggcg gtcgaccact tctgcaacta agagttgact ggctgcaatg ggggtcttta
    18121 tctgatggac ccacaaggta tagtaatcca gcaaatccgt cagttttctt tctgcttttg
    18181 acctctgctg atagagttcc atctcacgcg cttctaattt ttctgctaaa gctatttcca
    18241 aaggagactt ggcttccctc tctccataga gaagttcctg gcgatagacc tgcgtttcca
    18301 ccaatatgtc ccaagtgaaa aataatatgg ttacaaagca acacaagaag aaaaagtaga
    18361 ggaagtaaat tcctagactg gcaaataaaa actgaaagag taagacaaga aatgccaaag
    18421 aaagcagata gataaaaaga cgactacggg agcgcagata ggctagaaaa aattgtttcc
    18481 aatcaagcat gcttcaatcc gtaccctatt cctttcttgg tctcgataaa tcctaccaat
    18541 ccctgctcct ccaacttttt acgcaaacga gccacattga cagagagggt attatcatca
    18601 atgaaaaagt cactgttcca aagttcccgc atcaggtcgt cacgtgctac gatgttgcct
    18661 gcatgctcaa ataacacgcg taaaatctgg aattcattct tggtcaaatt caagacttgc
    18721 ccttgataat gtaaatccat ggatttggta ttgaggataa caccagcata ttccagcaaa
    18781 ctctcatcac gcccaaactc ataggaacga cgcaacaagc cctgaacctt agctaaaaga
    18841 acctgctggt caaaaggctt ggtcacaaag tcatccgccc ccatattgat tgccatgaca
    18901 atatccatag cctggtctct cgaagaaaga aacatgatag gtaccttgga aatcttgcgg
    18961 atttcctgac accagtgata accattaaac aagggcaaac caatatccat gaggaccaga
    19021 tgaggttccg actgaacaaa tagactcaaa acttccataa agtcttctac caggaccact
    19081 tcaaatcccc attcagagag cattttccca atctgttgac gaatgacctg atcatcttct
    19141 attaataaaa tcttgtgcat gcgcttctcc ttttctatta ttataacaga tttttccatg
    19201 ctagatggtc tgaaactgaa tttgaaatag cctgttttta gccagtacaa acaggctatg
    19261 ctactagcta atttgaggga aatttgctaa gataaataaa aagaaaggag ctcttatggc
    19321 caatattttt gactatctga aagatgtcgc atatgattct tattacgacc ttcccttgaa
    19381 tgagttagac attctaacct taatagaaat cacctacctc tcctttgata atctggtctc
    19441 cacacttcct caacgtcttt tagatctagc acctcaggtt ccaagagagc ccaccatgct
    19501 tactagcaaa aatcgccttc aattattaga tgaattggct caacacaagc gcttcaaaaa
    19561 ttgcaaactc tcccatttta tcaacgacat cgaccctgaa ctgcaaaagc aatttgcggc
    19621 tatgacttat cgtgtcagcc tcgataccta tctgattgtc tttcgtggga cagatgacag
    19681 tatcattggc tggaaggaag atttccacct gacctatatg aaggaaattc ctgctcaaaa
    19741 gcacgccctt cgctatttaa agaacttttt tgctcatcat cctaagcaaa aggttattct
    19801 ggccgggcat tccaagggag gaaatctcgc tatctatgct gctagccaaa ttgagcaaag
    19861 tttgcaaaat cagatcacag cagtttatac atttgatgca cctggtctcc atcaagaatt
    19921 gacacagact gcgggttatc aaaggataat ggatagaagc aagatattca ttccacaagg
    19981 ttccattatc ggtatgatgc tggaaattcc tgctcaccaa atcatcgttc agagtactgc
    20041 cctgggtggc atcgcccagc acgatacctt tagttggcag attgaggaca agcacttcgt
    20101 ccaactggat aagaccaaca gtgatagcca gcaagtagac acaaccttta aagaatgggt
    20161 ggccacagtc cctgacgaag aacttcagct ctacttcgac ctcttctttg gcactattct
    20221 tgaggctggt attagctcta tcaatgactt ggcttcctta aaggcgcttg aatacattca
    20281 tcatctcttt gtccaagctc aatccctcac tccagaagaa agagaaacct tgggtcgcct
    20341 tacccagtta ttgattgata ctcgttacca ggcatggaaa aatagataat actcttgaaa
    20401 attaaatgta tacaaaacaa aagacctaga atacatactt tcatgtgcat tctaagtctt
    20461 tttaaataga atctaatagt caataaaaat caaatagcat tgaaagataa ttgggcttgg
    20521 aacgtccctc tcgcttcaac aaaatgaccc cattatagat taaaaagatg ccacttagaa
    20581 aaagcaaaaa aggaagtaag acaaaggcaa atacataaaa agctaactga acattctcgt
    20641 atccattttt ataaaaaagg taggatagat aaaaataact tgaaatgagg gataataaaa
    20701 ataatactgg attccacaaa cttctattat ccttccaaaa tgacactata aaggctaata
    20761 caattcctat aacgagatac atttcttact cctttaatag ctacatttta tcataattat
    20821 ccaaagaaaa aagagggcat ttatccctct taatccttca tctgactctc tgcatcggcc
    20881 acgacttttt ctagactggt ttgaccaagt tctgcctcca tagtcaactg aattctctcc
    20941 aatttttgat ccaaaacatc atgaatatga gctcctacag ggcaatttgg attcggattg
    21001 tcatggaaac tgaagagttg acctgtctta ccaagacatt cgaccgcctg ataaacatct
    21061 aaaagactaa tatccttaag gtccttgaca atctctgttc cgcccgttcc acgcgctact
    21121 gaaatcagct ctgccttctt caactgggac aagatctttc tgataatgac aggattgacc
    21181 ccgacactag cagccagaaa atcactggtc accttgcttt ccttcccctc gagggcaatg
    21241 attatcagca tatgagtcgc aatggtaaat ctacttggaa tttgcatcct cttctccttt
    21301 ttacgaggct accctgcctc tactcttctt tttctattat tatacccttt ttagttgtaa
    21361 tgtcaatcgt taccactttt caaccagtcg tctaactccc gatcgcagcc ctctttctga
    21421 gccaattctc tcaaaaattc ctgatgatga gtatggtgga tcccattgac cagactttca
    21481 tagtaaacct caaaataagg aagtctcagg tctttagcca gctgcaattc agctgccaca
    21541 tcgtagtcta cccgtcggaa gtccatatct accaggccct tgtcatcaaa ctccaaaatc
    21601 atatactggg cccgcaagtc cttccgtagc tgagcgtcca aaaagaaagg ttggccaatc
    21661 gaacccggat tgacaatcaa ttgcccacca gtcccgtaac gaagcaactg ctggtgaata
    21721 tgtccataaa cagcaatatc acagggagga tgagtcacca agcggtcaaa ctcctcttgt
    21781 ttgccaatat gaatcaactc tcgcccccag ttcttatcag gcagatgatg gctaattccc
    21841 accgtcaaat ccccaaactg acgatgaatt tgaagaggtt gattgtggag cacttcaatt
    21901 tcttctaggg aaatttcctc taaaacatac tggcactggc gcaagagata gcgttgactg
    21961 gggcgagtac tatccaattc cttacggaca ccatgccaaa gactgtcttc ccagtttccc
    22021 aaaactctag ccgtaatcgg tagttgatcc aacaagtcca aaatccttct acgccctgtc
    22081 cctggcatga gaatatctcc caaaagccag tattcatcca ctcctatctg ccgagcatct
    22141 gccaaaacag cctccaaggc ggtggtattt ccatgaatat ctgaaagaag agctattttc
    22201 gtcatatcca tctcctcgtt ttttctcttg caataagtat aacataaaaa gtcacagcta
    22261 gagaaatcta gctttttttg atatactaga taaagatatt agacaagagg aaacgaatga
    22321 ccccaaacaa agaagactat ctaaaatgta tttatgaaat tggcatagac ctgcataaga
    22381 ttaccaacaa ggaaattgcg gctcgcatgc aagtctctcc ccctgccgta actgaaatga
    22441 tcaaacgaat gaaaagtgaa aatctcatcc taaaggacaa ggaatgtggc tatctactga
    22501 ctgacctcgg tctcaaactg gtctctgagc tctatcgtaa gcaccgcttg attgaagttt
    22561 ttctagttca tcatttagac tatacaagtg accagattca cgaggaagct gaggtcttgg
    22621 aacacactgt ctctgacctg ttcgtggaaa gactagataa actgctaggt ttccctaaaa
    22681 cctgccccca cggaggaact attcctgcca agggagaact cctcgttgaa atcaataacc
    22741 tcccactagc tgatatcaag gaagctggcg cctaccgcct gactcgggtg cacgatagtt
    22801 ttgacattct ccattatctg gacaagcact cacttcacat cggtgaccag ctccaagtca
    22861 agcagtttga tggcttcagc aataccttca ctatcctcag taacgacgag gatttacaag
    22921 tgaatatgga cattgcaaaa caactctatg tcgagaaaat caactaattt ctcaagtccc
    22981 ctaccaaccc tgaaagtttt attttcaggg ttttatttct atcattttat tttactttca
    23041 tttggtggta gaatatttga aaaaaatggg agagtatttg aaaaaaagaa aggtagttta
    23101 acctatgaaa aaatcactaa aaatttttgc tacatctaaa tgatttgacc tcttcggggt
    23161 tgctttggtc gttgggattg cgattgcatc tggttatctc aactcacgcc tcgataaatt
    23221 tgtagactgg ggactatgga cagctcttgt accctttgga ttgatttccg taaccaacgt
    23281 tgggatttcc atgttgtcca ctcgtttcac ggggaaatta agcaaatggg gaaattactt
    23341 tggtattgtt aataccattt tgtccggtgc tattgattat atccttggaa ataaggcggc
    23401 catcattacc tatcccgtca ccttactcat ttataccttt gctataaaga aatggaaagc
    23461 ttcgcaagaa ggcagaccca accaaatgag ccaaaaacag gtaaaattgg cggccatcat
    23521 catttccatc atcgccttcc tctttgcctt tgtgaccaac tatatcggat atgggggcaa
    23581 gatgaatctc cttgcctacg taacaactat tgcctttgca ctgtcgctca ttgccaatgc
    23641 tttgaatgca ttggcaatga gcgacagtgg ggcttttggt tgatttacaa tttcgttcag
    23701 ctgacaaaag ctggtattca aggaaacttc gccaatatcg gtaaatacat cttttatatc
    23761 ctcaatgcaa tcggagcttt atttgtctgg aatgatgaag aagttagata atagaaggaa
    23821 actcaaataa agaatactag ccaacttaag agaatctccc ctctgctatt tttatcattt
    23881 tagttgtaac tcccattctt ttagagtaga atgaagttag ataaaagagg gaggtcttgt
    23941 catgaaaaaa ctctatagaa tccattttat agcaattgca gttattgatt tgctactatt
    24001 tg
//
LOCUS       DAVHIT010000025        23832 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVHIT010000025 DAVHIT010000000
VERSION     DAVHIT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.025"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(98..2356)
                     /locus_tag="VVN16_000875"
     CDS             complement(98..2356)
                     /locus_tag="VVN16_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5572295.1"
                     /translation="MLCQNCKINDSTIHLYTNIDGKQKQIDLCQNCYKIIKTDPKNGL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VVN16_000876"
     CDS             complement(2585..2815)
                     /locus_tag="VVN16_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU5572296.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VVN16_000877"
     CDS             3005..3688
                     /locus_tag="VVN16_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5572297.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VVN16_000878"
     CDS             3688..4422
                     /locus_tag="VVN16_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5572298.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VVN16_000879"
     CDS             4576..5433
                     /locus_tag="VVN16_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU5572299.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="VVN16_000880"
     CDS             5575..6447
                     /locus_tag="VVN16_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5572300.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENILALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERARKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="VVN16_000881"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="VVN16_000881"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU5572301.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="VVN16_000882"
     CDS             7426..8637
                     /locus_tag="VVN16_000882"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU5572302.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="VVN16_000883"
     CDS             8639..9181
                     /locus_tag="VVN16_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU5572303.1"
                     /translation="MTCYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLGTFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9197..10006
                     /locus_tag="VVN16_000884"
     CDS             9197..10006
                     /locus_tag="VVN16_000884"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5572304.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10482..10877
                     /locus_tag="VVN16_000885"
     CDS             10482..10877
                     /locus_tag="VVN16_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU5572305.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10887..11462
                     /locus_tag="VVN16_000886"
     CDS             10887..11462
                     /locus_tag="VVN16_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU5572306.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11487..11999
                     /locus_tag="VVN16_000887"
     CDS             11487..11999
                     /locus_tag="VVN16_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU5572307.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12657..13367
                     /gene="deoD"
                     /locus_tag="VVN16_000888"
     CDS             12657..13367
                     /gene="deoD"
                     /locus_tag="VVN16_000888"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5572308.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13390..13914)
                     /locus_tag="VVN16_000889"
     CDS             complement(13390..13914)
                     /locus_tag="VVN16_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU5572309.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13967..14203)
                     /locus_tag="VVN16_000890"
     CDS             complement(13967..14203)
                     /locus_tag="VVN16_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU5572310.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(14271..15191)
                     /locus_tag="VVN16_000891"
     CDS             complement(14271..15191)
                     /locus_tag="VVN16_000891"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU5572311.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15381..15784
                     /locus_tag="VVN16_000892"
                     /pseudo
     CDS             15381..15784
                     /locus_tag="VVN16_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16000..16581
                     /locus_tag="VVN16_000893"
     CDS             16000..16581
                     /locus_tag="VVN16_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5572312.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16578..17855
                     /locus_tag="VVN16_000894"
     CDS             16578..17855
                     /locus_tag="VVN16_000894"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU5572313.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17873..18535
                     /gene="deoC"
                     /locus_tag="VVN16_000895"
     CDS             17873..18535
                     /gene="deoC"
                     /locus_tag="VVN16_000895"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5572314.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18522..18911
                     /locus_tag="VVN16_000896"
     CDS             18522..18911
                     /locus_tag="VVN16_000896"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5572315.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19002..20054
                     /locus_tag="VVN16_000897"
     CDS             19002..20054
                     /locus_tag="VVN16_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU5572316.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20198..21733
                     /locus_tag="VVN16_000898"
     CDS             20198..21733
                     /locus_tag="VVN16_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572317.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21726..22784
                     /locus_tag="VVN16_000899"
     CDS             21726..22784
                     /locus_tag="VVN16_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572318.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22787..23743
                     /locus_tag="VVN16_000900"
     CDS             22787..23743
                     /locus_tag="VVN16_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572319.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6938 a         4305 c         5414 g         7175 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttatca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata tggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggggcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggcccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatcggtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt catccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cacgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggcc attcttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccatcg atattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc cagcttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg aggccaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ctaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcaat caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccgc ttttatttgc
     3601 agcattttat tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggaatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag gttatctttg agggagtcga tattacggac
     3901 aagaagaatg acttgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc aacaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcaaatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaagaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggctggttc ccgtagcgaa gtagtgcgag ttccagagac cattactcaa
     4801 gaggaattgt tagacctgat tgctaaatat aatcaggatc cagcttggca tgggattttg
     4861 gtccagttgc cattaccaaa acatatcgat gaagaggcgg ttttattagc cattgaccca
     4921 gaaaaggatg tggatggttt ccatccccta aacatgggac gtctttggtc tggacatcca
     4981 gtcatgattc cctcgacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgac
     5041 ttggaaggta aaaatgcggt cgtaatcggt cgttcaaata tcgttggaaa acctatggct
     5101 cagcttcttt tggctaaaaa tgcgacagta accttggccc actcacgaac tcataatctt
     5161 gccaaggtgg ctgctaaagc agatattctt gtagtcgcaa tcggccgcgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggtt gtcattgacg ttgggatgaa tcgagatgaa
     5281 aatgggaagc tctgtggaga tgttgattat gaggcagttg caccacttgc tagccatatc
     5341 actccagtcc ctggaggtgt cggtcctatg accattacca tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaaac attcgattaa tagagtgtat
     5461 tttctatagc tatatctaaa atggtagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttattgta taataaaagt gagaggaaaa aaggatgaaa
     5581 gtgattaatc aaaccttatt agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc tggctctgca cagccattta cctgagccta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagttg tcttgctggg gcctggttta
     5881 cgagacgatg cttctggaga aaatctagta aaacaggtct ttgttaattt aagccaaaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagag
     6421 agggcaagaa aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 tttgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg
     6961 atgtgacggt tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggatcatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtaat cgttgctgga cgggatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacatat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaagcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcatatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataatat
     8641 gacgtgctat gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat
     8701 ggcggagctt cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa
     8761 cagtggcaat attttcttta cttcgataga ttccaaagcc caattggttg aaaagctagg
     8821 gactttcttt gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca gaagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt ggatcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caaggtcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcaga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 tagggcctac acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat
     9721 caagcttgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggctggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aaggggtagt gtctctgtct tttcaggatt gactgcctat ccgtattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtatgt
    10141 ctgatctgat agccagcatt gtgaaagaca agattctagg atactagcat tagctttcta
    10201 gccaagcaga ctagtatgat aaggagagat gagaatgaat tgactttctg aatttctcag
    10261 tcttatcata tatagcacaa tgagatttcg cttgagtctg cttgtaaata aacgaaaaga
    10321 aagataataa ataatgaaaa ttggtcaacg aattatgcgc tttggcataa aaaattaagt
    10381 atcggagttg tatctgttgt agtcggcttt gatttctagc tccagctgga atttcagcca
    10441 atgaagtaaa gcaagatgta acatctgaag tggtaatagg tgtgctagat tctaaggagg
    10501 aattgaaaga gtcagaaaat gatgctccaa aactagaaac tcctcttaga gaggagccaa
    10561 gactagctcc tcaaacgctt ccggaagcaa gtgaagttct tgaaaacaaa agggaagagt
    10621 caaaagtaga gataacagag ccagctcaag cggatgatat ccgcaaggtt gttggggaat
    10681 tagccaagga tataagtatt actaagttgt atatgacagg tcattctctt ggaggctacc
    10741 tagctcagat tgcagcggtt gaagattacc aaaaatatcc tgatttttat aaccatgtat
    10801 tgaggaaagt gacaactttc agtgctccta aagtcattac ttccagaact gtttgggatg
    10861 ctaagaatgg tttctgagat gttggtttgg aaagtcgtaa attagctgtt agtggaaaaa
    10921 ttaagcatta tgtggttgat aatgacaatg ttgtgactcc cttgattcat aataatcgtg
    10981 atattgttac atttacaggt aattcacgct ttaaacaccg ttctcgtggc tattttgaaa
    11041 gtccaatgaa tgatattcct aactttaata ttggtaaaca agctaccttg gataaacatg
    11101 gttatcgtga tccgaaattg gataaagtgc gattctttaa gaaacaggct ctacctcaat
    11161 cttctagtca accaagcgct gaaccaatgg aaaatattgc cttaggaaaa caggttactc
    11221 aaagttcgac agctttcgga ggagatgcta gaagagctgt ggatggcaaa gtcgatggta
    11281 actatggtca caattctgtc actcatacaa acttccaatc taagccttgg tggcaagtag
    11341 atttggctaa agaagaaacc attcgccaaa tcaatattta caaccgaaca gacactgccc
    11401 aggatagatt ggcaaacttt gatgtcattc ttttagacag ttctggtaaa gaaattgagt
    11461 gaaaacgtat aatatctcct aaagatgtgt cagcacaaat tacgattaac cataaaaaag
    11521 cgcgctatgt tcggattgag ctagaaggct ataatgccct cagtcttgca gaagttgaag
    11581 ttttccgctt tatagctacg aatgctgaaa cggcgacaca agtttctaag ccagttcaac
    11641 caatcagtca gactcctgtg aaagataaaa cattgacaat tcaacacagt ggagcttaca
    11701 ttgcccgcta ctccataact tgggaagaag ttccagtaga taaagatgga aaccaagttg
    11761 ttcgtagtca ttcttgggaa ggaaacggtt gcaaccagac tgcaggtttt gtcctcaacc
    11821 tcccaatcaa agaaaatatg agaaatctgc gagttaagat tgagaaaaag acgggcctac
    11881 tatggaatag atggcaaaca atctatgaaa acagaccaat tttagctcaa ccccaccgta
    11941 aaattaccca ttggggtacg acattgaatt ccaaggtgag tgacgatgat gtcttgtaat
    12001 ctgatggtag aatgacagtt agtttgtcta gtttataaga aagtactacc tgagcttgaa
    12061 tagaactcag gtagctctct atgaaagaac aaaattaata ctcaatgaaa atcaaagagc
    12121 aaactaggaa actagccgca ggttgctcaa agcactgctt tgaggttgta gataagactg
    12181 acgaagtcag tcacatatat aatccaaggc gacgttgacg tggtttgaag agattttcga
    12241 agagtataaa cagaaaggta gagcgcgtgt tctaatttga acacgagtag aaaacttttc
    12301 taaaaacaaa aacgaaagga tgggtaaact gtattcgctg aactgaatac gggcgactct
    12361 cctctaaatc aaaattaaga aagaaaggaa ttgcccccac cctaaaagca gtgggaaaaa
    12421 gatagttgat ctagcgagca tcgctcactg cgcccaactc ctattttccc ttcgcttttt
    12481 gatgggtttg gtatctttct caatataaaa tataaaataa agaaaggtag agcgtgtgtt
    12541 ttgatttgaa cacgagcgga aaactcggaa aatagataat ctgactgaaa aatcaggatt
    12601 tctcgtcagg ttcctaattt tcagtcgttt tcttctcgct ctttgtatca taaattatgt
    12661 ctatccatat tgctgctcag cagggtgaaa ttgctgataa aattcttctt cctggggatc
    12721 ctcttcgtgc taagtttatt gcggagaatt tccttgatga tgctgtttgt tttaacgaag
    12781 tgcgtaacat gtttggttac actggtactt acaagggtca ctgtgtatct gtcatgggaa
    12841 ctgggatggg aatgccatct atttcgattt atgcgcgtga gttaatcgta gactacggtg
    12901 tgaagaaatt gattcgtgtg ggaactgcag gttctttgaa tgaagaggtt catgttcgtg
    12961 aattagtttt ggcgcaggcg gctgcaacca actcaaacat cgttcgtaat gactggccac
    13021 agtacgattt tccacaaatt gctagctttg atttgcttga taaagcctac catatcgcca
    13081 aaaaacttgg tatgactact cacgttggga acgttttgtc atctgatgtc ttttactcaa
    13141 attactttga aaagaatatc gagcttggta aatggggagt caaggctgtg gaaatggaag
    13201 cagcagctct ttactatctt gctgcccaat accatgttga tgcgctagct atcatgacca
    13261 tctctgatag cttggtcaat ccagacgaag acacaactgc agaagaacgt caaaatacct
    13321 tcactgatat gatgaaggtt ggtttggaaa ccttgattgc agaataatta tagccaaaaa
    13381 ggaagccctt tatgaattgt aggacttcct tttcttatcc agaaattgat ctagctctct
    13441 ctgatttcga agaatagtga ctttatgtga atattcttgg caaagttttt ggtaattttc
    13501 tttttgagtt ttgctacgcc catcccaaag aatccatctg ataaactccc agtcaaagcg
    13561 ttcagggcaa tctgccgcca tactttctct gacttttcca cggtatttaa gataacgctt
    13621 aaaggctcta aagagacagg tcaatggcaa aaaattgaga aagatgattt ggtcagcttc
    13681 ttgcattcgt tcttggtagt agcaccaaga ataattacca tcgatgaccc aagctttatg
    13741 cttggtgaga aagtttttta tctcggttaa catccattcg cagtcactgt cttgccaacc
    13801 aggttgaaat tggagtgtgt ccatgtgcag ttttggaatg gagtagtagt tagataactt
    13861 ttctgctaga gttgacttac cagaaccaga atatccgata attgtgattt tcattttcta
    13921 ccttttccta tttggagaca aaaaaacagc ctctatggac tgtttcttat ttagcaagtt
    13981 tagttgaaag acgagcttta tcgcggcttg ctttgttttt gtgaatcaaa cctttagttt
    14041 ctgctttatc gatagctgag ctagcagcac ggaaaagttc ttcagatggg tttgcttcga
    14101 aagcttttat agcagtacgc atagctgatt tttgagctga gttcttttcg ttttgtttaa
    14161 cgttcaattc agcgcgtttg atagctgatt taatgtttgc caatgttctt acctccatat
    14221 ttactaacta taccattata tatgaaaact tacgttttga caagggaaaa ttactttttt
    14281 aagtaaattt catcgatttc atggttcttg cttttatgaa gaatcacttc tgcacgattt
    14341 ctggttggtt caatataatt ttgcagattt gtgagattga tactgatcca gacctgatgg
    14401 gcaaaggact ccacttcccc aatcggcatc tgagtaaaac gataatagta gctatcaggg
    14461 tcgttttggg ctaggctcag cattttcaag aaacggtcca gataccaact ttcaatatca
    14521 tccactccag catctacata gatggaaaag tcaaagaagt cagtgatata gagacgatcg
    14581 ttttgtggat tttgaaagac attaattccc tcaacaatta caaaatcagc agctttgaca
    14641 ctttgtttct tttcgggtac gatgtcgtaa acttcatgag aatagacagg aatatctaca
    14701 tcttgtccat ttttgatgcg gtccaagaag ttgagaagag cttccatatc atagctttca
    14761 ggaaatcctt tacgatttaa aatcccctgc tcaatcaagg tttgattggg atatagaaaa
    14821 ccatcagttg taaccaactc aaccgtagca tctgtaaacg tacgggacag taggatttga
    14881 agtaggcgac tggttgtgga ttttccaacg gcaacactcc cagaaacccc aataataaaa
    14941 ggttgagatt tactttcacg ttggaggaaa attccttttg aaaaggctaa atcttcctta
    15001 gttcgcttgt aaatctgaat cagatgagcc aaggggagat agatatctgt aacgtcttgg
    15061 agactgattt ggtcattaaa actcttgata gattccaatt cttcttctgt caaaggaggt
    15121 gttgtctttc gatgtaaaga ttgccaagtc tggcggctga ttttttcaaa atgtaaaaat
    15181 tcgttggtca tttgttttcc cctagatatt ttgtctatca taccataaaa agctaaaaaa
    15241 ataaagcggt ttcttgaatg tggtaagtca atggtatata gtataagtga tcaatttatt
    15301 gatgaaatag gagatagatt ctactgattt ataagcttat aaagggggct ttttagttca
    15361 caacttatag gaggtgtact atgaaaatct taaaacgtta catattggaa ctctgtttta
    15421 ttttaagttt tgctttacct tttataaaag gaaccaatgc agataatggt agatgctttg
    15481 tggaaaccta ttacggtttt acttttttga tggaacatgc tattgtaaca gctgtcttta
    15541 tctgttcgtt cttaattgct ttcttactaa aaaacgatgg acgaaatgga ttgctgcggg
    15601 tagttattgc tttttagtcc tacggattgc gacagagggt tatttcttcc acatgtcact
    15661 agaagatttg atacgacttt ggacaagttt ggaaatcctg acacaaacgt atcagttggg
    15721 cttttatcta aacattttgt taggaatttt gttgataata aagtatttta aggttaagca
    15781 atagaaataa taatctgcct gattatagat gataaacttt ttgtttttat ctggactgtg
    15841 atatttatag ttctaaatgg tttattattt ataagtgttg aaagagattg aagaaaatgg
    15901 cttcgtgact tgctttcttc ggtctttttc gttttattat caatttgtaa acgatttaca
    15961 atttgagtca aattatggta aaatagtgtc atgagtaaaa tgtactatgc agaaaatcct
    16021 gacgctgctc acgacattca tgagttgaga gtggacttgt tgggagaaaa aatgactttt
    16081 ttgacagatg cgggtgtttt tagcaagaaa atggttgact ttggaagtca actcttgctc
    16141 aagtgtctgg atgtcaacca aggagagaca gtcctagatg taggctgtgg ctatggtcca
    16201 ttaggtttgt ccttggtcaa ggtttacgga gttcaggcga ccatggttga tattaacact
    16261 cgtgccctgg atttagctca gagaaatgct gaaaaaaata atgcaaaagc gacgatattt
    16321 caatctaata tttatgcaag agttcaaggg cgatttgacc atgtcatttc caatcctcct
    16381 atccgtgcgg gcaagcaagt cgttcatgaa atcatcgaga aaagtaaaga tttcttggaa
    16441 attggtggag atttgaccat cgtcatccag aaaaaacaag gagctccaag tgccaagtct
    16501 aagatggaag atgtgtttgg caattgtgaa atccttaaaa aagacaaggg atactatatc
    16561 cttagaagtg tgaaagaatg agagcagttg atttaatcca aaaaaaacga gacggtcaag
    16621 aactgacttc aagtgaaatt gaatggctga tagaaggcta tgtatcagga actgttcctg
    16681 attatcagat gtctgccttt gctatggctg tttattttaa aggaatgacg actcgagaaa
    16741 tctctgattt aacgatgaat atggttaaga ctgggcaaga gtttgacctg tcagccattg
    16801 atggtgtgaa agttgacaag cattctactg gtggtgtcgg cgataaggta accttgattt
    16861 tagctcctct tgttgctagc ttcggtgtgc ctgtagcaaa aatgagtggt cgtggtcttg
    16921 gccatacagg cggaacaatt gataaattgg agtccattaa gggctatcaa gtagaacgta
    16981 gtcaagagga ttttattcgt caggttcagg atattggagt atctgtcatt gggcaatcag
    17041 accagctggt caaagcagat aagcttctct atgcccttcg tgatgtgacc gcaactgtcg
    17101 acacaattcc tttgattgcg agttcggtga tgagcaagaa aattgcggca ggggcggatg
    17161 ctattttgct agacgtgact gtcggtgagg gtgccttcat gaagacggtt gatgaggcgc
    17221 gtgagttggc tcaaactatg gtagatcttg gtaaggttgt tggacggaag acagtagcag
    17281 tgattacgga tatgagccag cctttgggac gagcgattgg aaatcgtctg gaaatccttg
    17341 aagcattgga gattttgcaa ggtcaaggac gtcaggatat tacccacttt atttgtgaat
    17401 tggctcaaat tatgcttggt ttggcaaatg ttaacaaaac tgttgaagaa gttcgccaac
    17461 atcttgaaaa tggtcaagca ctggctaagt ttgaggaaat ggtgcaagcc caaggtggag
    17521 acttggaaga cctctatcgt cctgtaaatg tagctcatgt ggtggaaatc cctgctcagg
    17581 aaacgggtgt catttcagct cttccagcta tggactttgg tctttatgcc atgaggctgg
    17641 gtgctggtcg tgcagttaag tctgatgtct tggactatga aacaggaatt gtttttgaaa
    17701 agaagattgg agactccgtt caaaagggtg aaattgttgc aaaagtatac acaaatgaaa
    17761 aaattccacc tcaactagtt acagatttcc aaaaatgtgt taaaataagt gatgaagtga
    17821 aaaaaatccg tgaaattgta gaaatcatct cctaaatgga gggaaaaccg aaatgaaatt
    17881 aaataaatat atagatcata cgcttttaaa acaagatgca aagaaaaaac aaattgatag
    17941 tttgttgtct gaggctagag agtatgactt tgccagtgtt tgcgttaatc cgacctgggt
    18001 tgaacatgct aaaaaaggac ttgaaggcac agatgttaag gtttgcacag tagtaggttt
    18061 ccctttggga gcaacaactt cagccgtgaa agcatttgag acaaaagaag ctatccaaaa
    18121 tggtgcagat gagattgata tggtgatcaa tgttggagct ctcaaatcag gtaatttagc
    18181 cttggttgag tcagatattc gcgcagtagt ggaagcaagt ggtgataagt tagtgaaagt
    18241 cattattgaa gcttgccttc tgacagacca agaaaaaatt gttgtttgcc aattggccca
    18301 aaaagctggg gctgactttg ttaaaacatc tactggcttt tcaactggtg gtgctacgat
    18361 agcagatgtt agattaatgc gtgaaacagt tggatctgat atgggagtca aggctgctgg
    18421 tggagctcgt tcttatgcag atgctcttgc ctttgtcgaa gcaggtgcga cccgtatcgg
    18481 aacgtcagct ggggtagcta ttttaaaagg agaattggca gatggcgact actgagttga
    18541 ttgaactggc aattgaaacc agcaaacatg cctatgtccc ctattctcac tttcctatcg
    18601 gagcagtttt agtagccaaa gacggaagtg tttacacggg agtgaatatc gaaaatgcta
    18661 gctatccttt gaccaattgc ggtgagagaa cagctgtttt taaggctata tctgaaggcc
    18721 aaagagaatt ttcagaattg attgtctatg gtcaaactga aaaaccgatt tcaccatgtg
    18781 gtgcttgtcg ccaagtaatg gtcgaatttt ttgaacaaga tttaaaagtg accttagtcg
    18841 caaaagataa atcgacggtc gagatgacgg tcggggagtt acttccatac tcttttacag
    18901 acttaaatta gtctgagtcg ctctttgagt ggcaagggtc tttgtgacca atcaatccat
    18961 acttgcaaca tcgttgcaca tcttatttag gaggttcagt aatgaacaag aaacaatggc
    19021 taggtcttgg cctagttgca gtggcagcag ttggacttgc tgcatgtggt aaccgctctt
    19081 ctcgtaacgc agcttcatct tctgatgtga agacaaaagc agcaatcgtc actgatactg
    19141 gtggtgttga tgacaaatca ttcaaccaat cagcttggga aggtttgcaa gactggggta
    19201 aagaacacaa tctttcaaaa gataacggtt tcacttactt ccaatcaaca agtgaagctg
    19261 actacgctaa caacttgcaa caagcggctg gaagttacaa cctaatcttt ggtgttggtt
    19321 ttgcccttca caatgcagtt gaagaagcag ctaaagaaca cactgacttg aactatgtct
    19381 tgattgatga tgtgattaaa gaccaaaaaa atgttgctag cgtaactttc gctgataatg
    19441 agtcaggtta ccttgcaggt gtggctgcag caaaaacaac taagacaaaa caagttggtt
    19501 ttgtaggtgg tatcgaatct gaagttatct ctcgttttga agcaggattc aaggctggtg
    19561 ttgcgtcagt agacccatct atcaaagtcc aagttgacta cgctggttca tttggtgatg
    19621 cggctaaagg taaaacaatt gcagccgcac aatacgcagc cggtgcagat attgtttacc
    19681 aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc aaaatctctc aacgaaagcc
    19741 gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgatcg tgaccaagaa gcagaaggta
    19801 aatacacttc taaagatggc aaagaatcaa actttgttct tgtatctact ttgaaacaag
    19861 ttggtacaac tgtaaaagat atttctaaca aggcagaaaa aggagaattc cctggcggtc
    19921 aagtgatcgt ttactcattg aaggataaag gggttgactt ggcagtaaca aacctttcag
    19981 aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat ccttgatgga agcgtaaaag
    20041 ttcctgaaaa ataatggatg gaagtcattt cttaggaagc ggccaagggc cgctttttta
    20101 agagttgaga caaagtcatt ttgtctcagt aaagcttgct actagacaaa ctagcaagtt
    20161 ttattgaaat aaaataagac tctgaaagga agagcacatg gcacacgaaa atgtcattga
    20221 gatgcgtgat attaccaagg tgtttggtgg atttgttgcc aacgacaaaa tcaacttgca
    20281 cctacgaaaa ggtgaaattc atgcactttt aggagaaaat ggggctggta agtccacgct
    20341 aatgaacatg ttagcaggcc ttcttgaacc aactagtggt gaaatcgcgg tcaacggtca
    20401 agttgtcaat ctcgactccc catctaaagc agctagcttg ggaatcggga tggttcacca
    20461 gcactttatg ttggttgaag ccttcacagt ggctgaaaac atcattttag gtagtgaatt
    20521 gactaaaaat ggtgtgctag atatcgctgg agctagcaaa gaaatcaagg ctctttctga
    20581 acgttatggc ttagctgttg acccttctgc caaggtagca gatatctcag ttggagccca
    20641 acaacgtgta gaaattttaa aaacacttta tcggggggct gatatcctta tctttgacga
    20701 accaacggct gttttgactc catcagaaat tgatgagttg atggctatta tgaaaaatct
    20761 tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa ttggatgaaa ttcgagcagt
    20821 ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt gaaaccgttg aaattgcagg
    20881 ggctaccaat gctgatttgg cggaaatgat ggtaggacgt tctgtttcct ttaaaacaga
    20941 gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc aaagatttgg tggtcaatga
    21001 aaaccgtggt gttccagctg ttaaaaatct gtccttggat gttcgtgctg gagagattgt
    21061 tggtattgcg gggattgatg gaaatggtca gtctgaactg attcaagcca ttacaggtct
    21121 tcgcaaggtt gaatctggta gcattgagct aaaaggagat tcaattgtag gcttgcaccc
    21181 acgtcagatt acagaactaa gtgttgggca cgttccagaa gaccgtcacc gtgatggctt
    21241 gattttggaa atgatgatat ctgaaaatat tgcccttcaa acctactata aagaaccaca
    21301 tagtaaaaat ggaattttga attattcaaa tattacttct tatgctaaaa agctgatgga
    21361 agagtttgat gttcgcgctg ccagtgaatt agttcctgca gctgcactct caggaggaaa
    21421 tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat cctgatctcc ttatcgttag
    21481 ccagccaact cgtgggttgg atgtcggtgc cattgagtat atccacaaac gcttgattga
    21541 agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt gaattggatg agattttaaa
    21601 cgtctcagac cgtattgccg ttatccacga tggtaagatt caaggtattg tatcaccaga
    21661 aacaaccaat aaacaagaac ttggtgtctt gatggctggt ggaaacttgg gaaaggagaa
    21721 gagtgatgtc taaaaaatta caacaaattt cggttccctt gatttctgta ttcctaggaa
    21781 ttttactcgg agccattgtc atgtggatct tcggttatga tgctatttgg ggctacgaag
    21841 aattgttcta tacagccttt ggcagtctgc gtgggattgg agaaatcttc cgtgctatgg
    21901 gtcctctggt cttgattggt cttggttttg ccgttgccag tcgagctggt ttctttaacg
    21961 tcggacttcc tggtcaggct ttggcaggtt ggattctcag tggttggttt gccctgtcgc
    22021 atccagatat gccccgtccc ttgatgattc tagcaaccat cgtgattgcc ttgattgctg
    22081 gtgggattgt cggagcgatt ccaggtattc ttagggccta tctagggacg tcagaggtta
    22141 ttgtaaccat catgatgaac tacattgtct tgtatgtagg gaatgccttt atccatgctt
    22201 tccctaaaga cttcatgcaa agtacagatt cgaccattcg tgttggggct aatgcaacct
    22261 atcagacacc ttggttggct gagttgactg gtaactcacg gatgaatatt ggtattttct
    22321 ttgccatcat tgccgttgca gttatttggt tcatgctcaa gaaaacaact cttggttttg
    22381 aaatccgtgc agttggtctt aatccacatg cttcagaata tgctggtatt tctgccaagc
    22441 ggactattat cctatctatg attatttcag gtgccttggc aggtcttggt ggagctgttg
    22501 aaggtttggg aaccttccag aacgtctatg ttcaaggttc gtcattagct atcggattta
    22561 acggaatggc ggttagtttg cttgcggcca actcaccaat tggtatactt tttgcagcct
    22621 tcctatttgg cgttctccaa gttggggctc ctggtatgaa tgcggcgcag gtaccatctg
    22681 agcttgtcag cattgtaaca gcgtctatta tcttctttgt cagtgttcat taccttatcg
    22741 aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa gtaaagatgt ctattataac
    22801 cttgctcccc ctcttggtgt cttctatgct gatttactca gcacctctca tctttacaag
    22861 tatcggtggt gttttctctg aacgtggtgg tgtggtaaac gttggccttg aaggaattat
    22921 ggttatgggt gccttttctg gagttgtctt taaccttgaa tttgcagaac aatttggagc
    22981 agcaactcca tggctatcct tgcttgtagc aggattggtt ggtagtgttt tctctatcat
    23041 ccacgcagca gcgacggttc atttccgtgc agaccatgtt gtcagcggta cggtattgaa
    23101 cttgatggcg cctgccttgg ctgttttctt agttaaagtt ctttataaca aaggacaaac
    23161 cgacaaccta agtcaaactt ttggacgctt tgatttccca gtcttggcaa atatcccagt
    23221 gattggtgat atcttcttca agtcaactag tctacttggt tatctggcga ttgccttctc
    23281 attccttgct tggtttattc tctttaagac tcaatttggt cttcgtctcc gctctgtcgg
    23341 tgaacatcct caagcagcgg ataccttggg aatcaacgtc tacaagatga gatatttagg
    23401 gattattatt tcaggttttc taggtggaat tggcggagcg atttatgcgc aatcaatctc
    23461 agttaacttc tcagtgacaa ctattgttgg acctggattt atcgcccttg ctgcgatgat
    23521 ctttgggaaa tggaatccaa tcggtgctat gctttctagt ctcttctttg gactttcaca
    23581 aagtttggct gttatcggtt ctcaattgcc gttcctacaa ggagtgccag cggtttatct
    23641 tcaaattgca ccttatgttt tgacaattct tgtcttagca gccttctttg gaaaagcagt
    23701 cgcaccaaaa gcagatggta tcaactacat caaatcaaaa taagcataca aaaaaacgtc
    23761 agtccaatga ctgaactgcc ccccaaaagt tagacagaaa aaatctaact tttggggtgt
    23821 ttttattatg aa
//
LOCUS       DAVHIT010000026        23154 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVHIT010000026 DAVHIT010000000
VERSION     DAVHIT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.026"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(1338..2561)
                     /locus_tag="VVN16_000901"
     CDS             complement(1338..2561)
                     /locus_tag="VVN16_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU5572320.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            2729..4051
                     /locus_tag="VVN16_000902"
     CDS             2729..4051
                     /locus_tag="VVN16_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5572321.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            4053..5939
                     /locus_tag="VVN16_000903"
     CDS             4053..5939
                     /locus_tag="VVN16_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU5572322.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRRDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRNYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            6107..6451
                     /locus_tag="VVN16_000904"
     CDS             6107..6451
                     /locus_tag="VVN16_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5572323.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            6461..7873
                     /gene="lacG"
                     /locus_tag="VVN16_000905"
     CDS             6461..7873
                     /gene="lacG"
                     /locus_tag="VVN16_000905"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU5572324.1"
                     /translation="MENLQFKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            7943..9622
                     /locus_tag="VVN16_000906"
     CDS             7943..9622
                     /locus_tag="VVN16_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5572325.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QPISFVLLSLMLVVDILIYLPFCRAYDRQLLMKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(9748..11187)
                     /locus_tag="VVN16_000907"
     CDS             complement(9748..11187)
                     /locus_tag="VVN16_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU5572326.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLITLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(11191..12540)
                     /gene="trkA"
                     /locus_tag="VVN16_000908"
     CDS             complement(11191..12540)
                     /gene="trkA"
                     /locus_tag="VVN16_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU5572327.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIEREHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12729..13577
                     /locus_tag="VVN16_000909"
     CDS             12729..13577
                     /locus_tag="VVN16_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU5572328.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            13592..14140
                     /locus_tag="VVN16_000910"
     CDS             13592..14140
                     /locus_tag="VVN16_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU5572329.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            14147..14359
                     /locus_tag="VVN16_000911"
     CDS             14147..14359
                     /locus_tag="VVN16_000911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572330.1"
                     /translation="MKIKEKTRKQVAGCSKHGFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            14461..16143
                     /locus_tag="VVN16_000912"
     CDS             14461..16143
                     /locus_tag="VVN16_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572331.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQIQLQTELVKETPALQSLFILEEVSFSYNDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVNEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            16128..16958
                     /locus_tag="VVN16_000913"
     CDS             16128..16958
                     /locus_tag="VVN16_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU5572332.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            17151..17654
                     /locus_tag="VVN16_000914"
     CDS             17151..17654
                     /locus_tag="VVN16_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU5572333.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      17708..17942
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            18107..18670
                     /locus_tag="VVN16_000915"
     CDS             18107..18670
                     /locus_tag="VVN16_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5572334.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVHAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIQGVIFSVSFWLLYVLLKPTLKHYER"
     gene            18684..19310
                     /locus_tag="VVN16_000916"
     CDS             18684..19310
                     /locus_tag="VVN16_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5572335.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            19337..19726
                     /locus_tag="VVN16_000917"
     CDS             19337..19726
                     /locus_tag="VVN16_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU5572336.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRGGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            19731..20180
                     /locus_tag="VVN16_000918"
     CDS             19731..20180
                     /locus_tag="VVN16_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572337.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            20308..20895
                     /locus_tag="VVN16_000919"
     CDS             20308..20895
                     /locus_tag="VVN16_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU5572338.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            21285..22892
                     /locus_tag="VVN16_000920"
     CDS             21285..22892
                     /locus_tag="VVN16_000920"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5572339.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6916 a         3879 c         5042 g         7317 t
ORIGIN      
        1 aatcttcttg ttgctttaaa tcacaatcga aaaattctgt ttttcatctt agaaaagaat
       61 gtttagaaat gtaaaatgaa ccttttttct tatccgaaaa ccttactaaa gtatataatt
      121 taggaagtca atcctgtatg ataatgtttg tactatcgtc gagtttttgc ttgattgcct
      181 aacgagataa attttagaag aattttcggt gtgatttgac aagcttctga ctggaaggat
      241 agttacacta gtattgttta ttaaaatgag cagatagaga aaatttggaa tctatttgct
      301 tattatcaat cgttttttta gcttatttgt cttagattgg tatagaactg atctatggat
      361 taatcctctt gtatcttatc ctgttcctaa atatgttggt agaaaaatga agcagttgtt
      421 acgagtgtct gtattggaaa aaattttgtc tatagaattc tttaaatgat atgaaggcta
      481 ggaatgccaa agcactgaca tatttcgttt gaatctcatc cgctctcttt acttgtattt
      541 gtagaaatgc tatcagtaat atattcgcga acactatgat ttacaacagt gtggtcgtac
      601 atctttgtaa ctttattata caacaaccat ttacaggtgt aaagttgagc agtgtcttta
      661 gtaaatttag ggatttgttt tgtcatagtt ttgatacctg tattcgttgt ttcatgagca
      721 tttgacgttt cttttcatgt aggcatttca acaattagaa tgatattttt ccgaggatga
      781 acttatatat tgtgactttt tagagttatg atgcctcttg atttgactat cgctatcaat
      841 ttcatccact tttacagttt catggtttta tgaaccgaaa catctaggta caggttttag
      901 catcaattga ttgtttttga gccatatgaa gttgaaacgt ggagatgctt ttcttgtgat
      961 aacgccctat tcaaagagga ataaccgttt ttggagagag ttcagtacta tgtggcgcta
     1021 aatactatat gtaaaacata tattaaacaa gatttaacta aggagaattg taaaaacaaa
     1081 aatgagaatt tcatataaag tttattctag gagtatgtat cttgaataaa aattgttatt
     1141 taagttctgg gggttatgat gaatgttttt ataataataa aatgcagcta gaagaaaagt
     1201 tattttaata taaatcactc aacatagcga gaaggcggtt tcctatatgg aagtcgtctt
     1261 gttttcatat tgtctaaata cgagatatct actacaggtt atagttgatt taaacaggtc
     1321 tatagatttc taatatatta aggtgaaata atatcttgta atacattgct atgttctatg
     1381 aaaaaagcga cgatagctaa tgcacaagct cctatacttc cacgatgttt gttgtaaggt
     1441 aaaatttcaa tttctatatt acgagtaaag ggaacgaagt ggagctggtc ttggatttta
     1501 tccctgactt catgagtgaa aggttgataa ttaagcaatt gactgttgat atagatttta
     1561 tcagcaggat tgatgaggag gagatttgca atagaagtgg ctaataaatt gactccttta
     1621 tcaatttgtt gacgtaaagc ggagtcgcct aaattataag ccgttaaaag ggtgtctaaa
     1681 tgaatgtctt tttcagtctt tacaaggctt tttagtacag ttagttggga atttttaaat
     1741 aataattggg cgtgtttgat taaccaagca tccgaaatat atgtttgtaa acaacctttt
     1801 tttccgcatt cacaatattg cccattcaat tcgacaatgg tgtgtccaat ttctccgata
     1861 taaggatttt tagaggctcc tattttttct tttgtgaaaa aggaagtgta aatacctaat
     1921 ccagcgtgta ggaaaataaa gttatcgggg gtattgtgtg gattaaaaag gtattgtcct
     1981 atagccatac aatcgatatt attttttaca ataaaaggaa aattgaatct tttaattaca
     2041 tttaataaat taaaagattc ccatatgggg ttgtttgtaa tgatacttcc ggagtctttg
     2101 ttataatgtc caggtattga taagccaaat ccaagtaatt ttgtttccaa agggacgtac
     2161 tctatagctg tttttatcat attggtaatg gtggaattga tttctgaaat aggataatct
     2221 tcattcaaaa tttctgtctc gaaaagagtt ttcccttgta agtcacatag actgagaaag
     2281 atacctagga cgtttatttc aatacctcca acaacatacg gaatattatc tgtgatagaa
     2341 atagtttttc tacgtcttcc agatccggaa aactcgctaa attcatcacc agtttcgtat
     2401 atgagggact gttcgcttag ctcttttatg atgttagtcg ttgttgctgg agtaagtcct
     2461 gtttcattaa caatatctgt acgggatgtt gctggatttc gatatagaaa ctccagtata
     2521 gtggctctat tttgtaaaaa ggctgattgt tttgtaatca tttgtttcct cctttggttt
     2581 attttatatt aaacataaga aaaaatcaat taaaattatt attctataca aagaaaaaaa
     2641 tattgtaaaa acaacaaaaa atatattgac aacgctttct gttagtggta tatttaattt
     2701 ataaaaaata aaaaaaggag ttccggtgat gaacaatatt ttagcgtttt tagaaacaaa
     2761 agtcgctccg tttggtgaaa aagttggcaa ccaacggcat ttgaaagcta ttcgtgaagg
     2821 atttatgatg gcaatgcctt tgattttagt cggatcttta tttcttattc taatcagttg
     2881 gcctcaagag gattttacaa attggctgaa tagtgttgga ttgctaagta tcttgacaac
     2941 tatgaatcag tcaacagtag cgattatctc cttggtcgct tgtttcggta ttgcctacag
     3001 gttgtcggaa ggatatggta cagatggtcc gtcggcaggg atcatagcct tatccagttt
     3061 tgtattgatg gcacctcgtt tttcgagtat ggtttatgat aaaaatgggg agcaggtcaa
     3121 gcagttattt ggcggcgcaa taccattttc tagcctgaat gcatcttctt tgtttatggc
     3181 gattactatt ggattggtta cagcagagat ttatcgtatg tttatccagc gcggaattac
     3241 gataaaaatg ccaagtggtg tcccagatgt ggtaagtaaa tcattttcag ctcttttacc
     3301 tggctttact acttttgttt tgtgggcttt ggtcttaaaa ggtcttgaag cggcaggagt
     3361 tgcaggaggt ctcaacggac tcctaggtgc aattgttgga acaccgctta agttaattgc
     3421 aggaacgctt ccaggtatga ttctatgtgt tattgtaaac tcattctttt ggttctgtgg
     3481 agttaatggg ggacaagttt taaatgcttt tgtagaccca gtttggttac aatttactac
     3541 agaaaaccaa gaagctgtgg ctgcaggaca aacactccaa cacattatta cattaccgtt
     3601 taaagattta tttgtattta ttggtggcgg tggagcgact attggtcttg cgatttgtct
     3661 cttcctattt agtaagagtc gtgcgaataa aacattaggt aagctagcta ttataccgtc
     3721 tatttttaat atcaatacag ctattctatt tacgtttcca acagttttaa atccgattat
     3781 gctgattccg tttattgcta ctcctacaat caatgccttg attacctatg tatcaatggc
     3841 tgtaggatta gtaccctata caacaggtgt aatccttccg tggacaatgc caccgattat
     3901 aggaggcttc cttgcaacag gggctagttg gcgaggagct ctattacaag ttgttttgat
     3961 tttggtttct gtagcaattt attatccatt cttcaaaatt gcagataaac gcaatcttga
     4021 aaaagaaaaa gctactgttg gagggaaata agatggttat cagagtattt gatcagcaga
     4081 aaaatactta ttctagcttt gccttagagg aattaagtta ctatataaat cgggtcttta
     4141 agactgacat agaacttgtc gaggagaagg aagcggatat ttttgtagga ttagtcaata
     4201 aagaggatag aagagaccat gttcttatct cattagacaa gggtaagggg agaattgagt
     4261 ctaatacaat tgtaggttta cttattggaa tttaccgaat gtttcatgaa tttggggttg
     4321 tgtatactag accagggcgc ggacatgact ttgttccaga gttacgattt gaagattttt
     4381 tagataaaca gctatctata gatgaaacag ccagttacta tcatagggga gtatgtatag
     4441 agggagcgga ttcatttgaa aatatactag atttcattga ttggctacct aagattggga
     4501 tgaacagttt tttcatccag tttgaaaatc cctactcttt tttgaaacgt tggtatgaac
     4561 atgaatttaa tccatatcta aataaagaac aattttcaaa tgaattagta caagaattga
     4621 gtgataggtt ggataaagaa ttgcaaaaaa gaggtcttat tcatcatcgt gttggtcatg
     4681 gatggacagg tgaagtttta ggttactctt caaaatttgg ctgggaatca ggtcttagta
     4741 tttcagagga gaagaaaccc tatgtcgctg aaataaacgg gaaacgagaa ttgtttaata
     4801 cggctccgat tttaaccagc ctggattttt caaatccaga tgtagctgat aagatggtag
     4861 aaattatcaa agattatgcc aagaaaagac ctgatgttaa ctacttacat gtatggttgt
     4921 cggatgctcg taataatatt tgtgaatgcg aaaactgtag acaagaattg gtttcggatc
     4981 agtatattcg tattctcaat caattggata gggctttaac gagtgaggga ttagatacaa
     5041 agatttgttt tctgctttac catgagttgt tatgggcgcc tcagaaagaa aaattagata
     5101 atcctgaacg ctttaccatg atgtttgcac cgattacaag aacatttgaa atgagttatg
     5161 cagatgtaga ttttgacaat tccataccta cgcctaaacc ttatctgcgt aataaaatta
     5221 tacttccgaa ttctcttgag gaaaatttat cttatctttt tgagtggcaa aaagcattta
     5281 aaggagatag tttcgtatat gactatcctt tagggcgtgc tcattatggc gatttaggct
     5341 atatgaaaat tagtcaaact atttacagag atgtatctta tctttccaac ctacatttga
     5401 acgggtacat ttcgtgtcaa gaattacgtg ccggattccc tcataatttt cctaattatg
     5461 tcatggggca aatgctctgg aagaagacaa gaaattatga agaattgatt gaagaatact
     5521 tttctgcttt gtatggggaa aattggcagt ctgttgttga atatttagaa aaattatcca
     5581 tttattcctc ttgtgattat tttaatgcaa ttggcagccg tcaaagtgat gttttagcga
     5641 atcattatta tatagcttac aatctagctg ataatttttt accaattatt gaggaaaata
     5701 tttctaagtt attaaatagt caaaaggatg aatggaaaca gctcagttat catcgtgaat
     5761 atgttgttaa gatggcgaag gctttatatc ttcaagcaac tggaaaaaca aggcaagctc
     5821 aagatgaatg gagaaatgtg ttgaattata tccgtgggca cgaattgcta tttcaatcta
     5881 atttggatgt ttatcgtgta attgaagtag caaaaaatta cgctggtttc cacttataaa
     5941 tcataagtat agaaaatgaa ctaaggtatt cagagaagat tgaccctaaa tattatgaaa
     6001 tttaaggatt tttaagatat ttagggtcga ctttctattt gtatcgtagc gaagtcattt
     6061 taataatgat gtgtaaaaga tggatcaaga ttgaggagga agaaagatga aatcaaaaga
     6121 agaaataaat atgcttggtt ttacaattgt cgcttacgca ggagatgcaa ggtcagattt
     6181 gatggatgct ttggcgtttg cgagagatgg atattttgaa caggcaagag aattggttga
     6241 gtctgcaaac gactcaatag tgtctgccca tcgagaacag actaatttat tagcggagga
     6301 ggcatatgga gataattttg aagtgagctt tattatgatt catggtcaag atactttgat
     6361 gacaacgatg ctattgtatg atcaggtaaa gttttttatt gatgaatatg aacgaattcg
     6421 aaagattgaa gaacatattg gtttgcaatg aggattagtc atggaaaatt tacagtttaa
     6481 agccttaccg aaggagtttt tattaggagc tgctaccgct gcttatcaag tagagggtgc
     6541 aactagggta gatggcaaag gaataaatat gtgggatgtt tatttgcaag aaaatagtcc
     6601 gttcttacca gatccagcta gtgattttta ttatcgttac gaagaggata tagctttggc
     6661 ggcagaacat ggtttgcagg ctttgcgttt atctatttct tgggttcgta tatttcctga
     6721 tatagatgga gatgctaatg tattagctgt tcattattac catagagttt ttcagtcttg
     6781 cttaaaacat aatgtgattc cgtttgtttc tttacatcat tttgattcgc ctcagaaaat
     6841 gttagaaaca ggggattggt tgaatagaga gaatattgat cgtttcatac gatatgctcg
     6901 cttttgtttc caagaattta cagaagtcaa gcattggttt acaatcaatg aactgatgtc
     6961 tcttgctgca ggtcaatata taggaggtca gtttcctcca aatcatcatt ttcaattatc
     7021 tgaagcaatt caagcgaatc ataatatgtt gttggcgcat gctcttgcag tcctcgaatt
     7081 tcatcaatta gggattgagg gaaaggtagg ttgtattcat gctttaaagc caggctatcc
     7141 tattgatggg caaaaagaaa atattttggc agctaaacgg tatgatgttt ataataataa
     7201 atttctatta gatggaactt ttttgggcta ctacagtgag gacacgcttt ttcacttgaa
     7261 tcaaatattg gaagctaata attctagctt tattattgaa gatggtgatt tagaaattat
     7321 gaagagagct gcagctctta atacgatgtt tgggatgaat tattatcgtt cagaatttat
     7381 tcgtgaatac aaaggtgaaa atagacaaga atttaattca acaggaataa aaggacagtc
     7441 ttcttttaaa ttaaatgctc taggtgaatt tgtaaaaaaa cctggtattc cgacaacaga
     7501 ttgggattgg aatatttatc ctcaagggtt atttgatatg ttgcttcgta tcaaagaaga
     7561 atatcctcaa catccggtca tttatttaac tgaaaatggt acagccctta aagaagttaa
     7621 gccagagggc gagaatgata ttattgatga cagtaaaaga atccgttata ttgagcaaca
     7681 tttacacaaa gttttagagg ctcgagatag gggagtcaat attcaaggct attttatatg
     7741 gtctttgcaa gatcaatttt cttgggcgaa tggctacaat aagcgatatg gtcttttctt
     7801 tgttgattat gaaacacaga agagatatat taagaaaagt gctctttggg taaaaggatt
     7861 aaaacggaat taaggttagc gatttgactg atgtttaata tattttaact atgagcttaa
     7921 attttttaca ggaggagttt ttatggataa gttagtcgct gccattgaaa agcaacaagg
     7981 gaaatttgaa aaaatttcta ctaataatta tatgatggct attaaagatg gatttattgc
     8041 tactatgcct ttaattatgt tttcaagctt tttgatgatt attattatga ttcctaaaaa
     8101 tttcggagta gagttaccga gtccagctat tgcctggatg aggaaagtgt atatgctaac
     8161 catgggagtt ttgggtgtta ttgtttcagg gacagttgca aagtcattgg ctggaaatgt
     8221 taatagaaaa atgcctcctg gaaaggtaat aaatgatact tctgcaatgt tggcggccat
     8281 atgtggctat cttgtattaa ctgtaacgct tgtagttgat gagaaaacgg gatctacaag
     8341 tttgtcgaca aactatttag gatctcaagg attgataact tcgtttgtca gtgcctttat
     8401 tactgtaaat gtttaccgat tctgtattaa gcgagacatt actattcatt tacctaagga
     8461 agttcctggg gctatatcac aagcttttag agatattttt cctttttctt ttgttttact
     8521 tattagtggt ttgttagata ttgtatctcg gtttagttta gatgttcctt ttgcccaagt
     8581 atttcaacaa ctattgactc ctatttttaa gggggcagaa tcatatcctg ctatgatgtt
     8641 gatttggttt atgtgcgctt tgctttggtt tgttggaatt catggaccat ctattgtctt
     8701 acctgctgtt acagctttac aactgagcaa tatggaagag aatgctcaac ttcttgcaaa
     8761 tgggcagttt ccttatcatt ctttaacgcc taatttcgga aattatatcg ctgctatcgg
     8821 aggaacgggg gctacctttg ttgtaccatt tattttgatt ttctttatgc ggtctaaaca
     8881 attaaaatcg gtaggtaaag ctacaattac tcctgtttta tttgcggtga atgaacctct
     8941 tctatttggt atgccagtta ttttgaatcc ctatcttttt gtaccttttt tgatgactcc
     9001 accagtaaat gtatttctag gaaaggtatt tattgatttc tttggaatga atggatttta
     9061 tatccagtta ccttgggctt ttcctggtcc cttgggattg ttaattggaa cgaattttca
     9121 acctatctcc tttgtgcttt tatctttgat gttagttgtc gacatattga tttatttgcc
     9181 attctgtaga gcgtatgata gacagttact gatgaaagaa gatattgcaa gctcaaatga
     9241 tattatttta gaggaggata caagtgaaat aatgcctggt gagatagatg taataaaaag
     9301 taaggagttg aaagtactgg ttctttgtgc agggtctgga acaagtgcgc aattagccaa
     9361 tgcaattaac gagggcgctc aattagcaga agttagagtg attgcgaatt caggagcgta
     9421 cggagctcat tatgatatta tgggtgttta tgatttaatt attctggccc cacaagttcg
     9481 gagttattat agagagatga aggtggatgc agaaagatta ggtattcaga tagttgctac
     9541 cagaggaatg gaatatattc atttaacaaa gagtccaagt aaagccttac aatttgtatt
     9601 ggagcattac caagctgtgt agtaagtttt tccatctttt atttgagtaa agattttgct
     9661 tacagatagg cttggattta aaaacgttcc ccctttttta atataagaat ccctctttca
     9721 caattgtaaa aagagggatt ttgtatttta tctcttagac caagttctct tcataaagag
     9781 aagtaggatt gggtaaatct ccaagcggcc tgcaatcatt gcaaaggaga ggagaatttt
     9841 tgagatagga ctaaagattg agaaactaga agtggttcct agaataggcc cgatattatt
     9901 gaaacagcta aagacagcgc tggtcacgat cagaaaatca ttgctatcta ggctgacaat
     9961 aaagataagg gatagcaaaa tcatagcata gatgacaaag tacttgagaa ttttatgctg
    10021 ggtatcctta tcaatcaccg ttttattcac atggagagtc aaaacacggt ggggagatag
    10081 aatggacaaa atttgatttt tggcaatttt tgaaaggatg aggcctcgaa taatcttgag
    10141 tccacctgca gttgatccag cagaaccacc gattgtcatg aggaagagga ggataaactg
    10201 ggagaagagg ggccagttgg taatatctcc atatccaaaa ccagttgttg taatgatgtt
    10261 ggaaacctgg aagaaggcca tttcaaagct tttggaaaat cctgggtaga ggtagagagt
    10321 attgagggta atcaagcctg tagaaaccag cacgatgacc aagtaagccc gaagctcttc
    10381 atctccaaag aaggccttaa cccgacggag cataaggtag tagtagagat tgaaatttac
    10441 cccaaaaacc aaaactccta tactaaccag ataggtaatc agtgagctac catagtgggc
    10501 aattccatcg ttatagacgg taaatcctcc agttcccgct gtccccatag caatgacaaa
    10561 actatcgtag agaggcatac cggctagata atagatgatg acaaagaggg aaaagagggc
    10621 tatgtaaagg agatagagga tctgggcagt gttttttaat ttggatacaa ccttaccaaa
    10681 gacaggacca ggaacctcag ccttcattac ttctaggtga ctatttttgg cattgtccat
    10741 aatagcaagt gcaaaaacaa gcactcccat ccctccaatc aagtgggtaa aacttcgcca
    10801 gaagaggagg gaacggctga gaaccgaaat gtcgttcaaa atacttgctc cagtagttgt
    10861 aaacccagaa ctaatttcaa aaaaggcatc aataacgcta gggatttgcc cagaaaagac
    10921 aaaggggaga ccaccaaaga aagaccaaag gatccaacag agggcaacga tcaagactcc
    10981 ctccttggca taaatccgtt gattttttgg cttctgtaaa ctccctgaac cgcctaacaa
    11041 tacgagaatc cctatggtcg aaaagagggc tgtaaagact tggctcgatt cacggtaata
    11101 gacagcgata gccacaggaa ccaagaggag aacagcttca atcaaaagta actttgaaag
    11161 gaggtaacga atcatacttt tattcatttc ttacctcgca atcaagtcat aaatcttggt
    11221 gatgtttggc aacaaggttg ttactaggag cttgtctcca acttccaaca tatcctcccc
    11281 agttgggaaa atagtcttgc cctttcgaat aatggctgca ataagaaccc cttttttcaa
    11341 tttcagttga gaaagaggtt tggcagtcat tttattggct tccttgatat ggaattgcag
    11401 ggtttcgatt tggccattgg ctagatggtg catggcttga aggtctgaat actgggcatt
    11461 aactcgacca cgaataaagt gcataatcgt atctacagcg atgcttttag gtgtgatgat
    11521 acttgaaaaa tcaggcgcat tgataatctc gaggagactg gtacgattga ccttagtaat
    11581 atttttctgt acacctaccc tgtcaaggaa catagatgta atcagatttt cctcatcgac
    11641 acctgttaga gtcgcaacgg catcatagtg ttgagcactt tcttccagca ggatatcttt
    11701 tgcggttcca tctccttgaa cgatgtagag atttgggaat ttctcgctaa agaagctggc
    11761 gatttcagga ttgatttcaa tgacttttgt atcgatacga ctatctttga gaataccaag
    11821 tagataatag gcaattctac ctgccccaac gatgagaagg ctcttcacgg cgcgtgattt
    11881 aaaataatta tggaagagta tcatatcgac acggttacca gtgacaaaga ttctatcttt
    11941 atcctgtaca gtcatgtcac cgcttggaat gataatttga tgctccctct ctatcgcaca
    12001 gacaatgaca ttaccaaatt ttttacgaaa atcagaaatg ggcatttggc aaagaccgct
    12061 ggtggacttg acgacaaatt ccatgaggct aacgcgtcca ccagcaaagc gttcgacaga
    12121 cagggcgttg gggaagtcaa tgatattcgc gatagcgcgg gcagccaaga gctcaggatt
    12181 aacgataaga gaaaaaccga gaatattctt ttccttgaaa taagagttag aatattcagg
    12241 gttccgcacc cgaacgatag tttctttagc tcccattttc ttggctagaa ctgctgcaat
    12301 catgttgact tcatcgtgct cagtcagggc gataaagata tcacaatctt ggacgctggc
    12361 ttgctcaaga atggcaaaat cggccccgtt accaaggata ccaatgatat caaagcgact
    12421 gacaatatga ttgagaacag cttcgtcttg ctcaatcagc aaaacatcat gcttttctgc
    12481 aaccaaggag cgacagaggg caaaaccaac ttttccccct ccgacaagga taattttcat
    12541 aataaaacct actttttcat gatgtaacta tcataccctt tttcaagaaa aaatgcacct
    12601 actagctaat aaccagagtt tttagtagga aatttgctat aagttaaaac tataccctaa
    12661 ccaattgaaa tagctattag cgactttctc tgaaatatgg tatgataaag gatatacaag
    12721 gagataaaat gaataataat ttactggtat tacaatcaga ctttggtctg gttgatggtg
    12781 cggtatcggc tatgattgga gtggctttag aagagtctcc aaccttaaaa atccatcact
    12841 tgacgcacga tatcacgcct tataatattt ttgaggggag ctatcgtctc tttcagacgg
    12901 tggattactg gcctgaggga acgacgtttg tatcggttgt cgatccaggt gtcggttcga
    12961 aacgtaagag tgtagttgcc aagactgcaa aaaatcaata cattgtcacg ccagataatg
    13021 ggacgctttc ctttatcaag aaacacgttg gcattgtagc cattcgtgag atttctgagg
    13081 tggccaatag gcgtcaaaac acagagcatt cttatacctt ccacggtcgt gatgtctatg
    13141 cctatactgg tgctaaactg gccagtggtc acattacttt tgaggaagta gggccagagc
    13201 tcagtgtgga acagattgta gagcttccag tcgtagcgac catcatagaa gatcatctgg
    13261 tgaagggagc cattgatatt ctggatgtgc gtttcggttc gctttggacc tctatcacac
    13321 gggaagaatt ttacaagctg gaaccagaat ttggtgatcg ttttgaagtg accatctatc
    13381 atgctgatat gctggtctat caaaatcagg ttgtctatgg caaatcattt gcagatgtga
    13441 gaattgggca acccatcctt tacatcaact ctctctatcg tttaggtttg gctatcaacc
    13501 aaggttcctt tgccaaggcc tataatgtag gtgtcggttc atcttggacc attgaaataa
    13561 agaaaataga aggataaaat aggagaatag tatggaaatc aaatttacaa ttaaacaagt
    13621 tgttgctgtc ggaattggcg ctgccctctt tgtcgtcatc gggatgatca acattccaac
    13681 ccctgttcca aatacaagca tccagcttca gtatgcggta caagcgctac tttctattat
    13741 ttttggaccg attatcggtt tgcttgtcgg gttgattggt catgcaatca aggactctct
    13801 tgttggttat ggtctatggt ggacttggat tatcgctagc ggactctttg gcttagttgt
    13861 gggacttttt agaaagtacg ttcgcgtgat caatagtgtt tttgactgga aagatattct
    13921 tatttttaat ctcattcaac tacttgcaaa tgcccttgtt tggggtgtct tggcaccact
    13981 tggagatgtt gtgatttatc aagaagcggc agaaaaagta tttgcacaag ggattgttgc
    14041 gggaattgcc aatggtgtat ctgtagctat tgcaggaact cttctcttac ttgcctatgc
    14101 aggaacccaa actcgtgcag gaagtttgaa aaaggactaa tactcaatga aaatcaaaga
    14161 gaaaactagg aagcaagtcg caggttgctc aaaacatggt tttgaggttg tagatgaaac
    14221 tgacgaagtc agtaaccata cctacggcaa ggcgaagctg acgtggtttg aagagatttt
    14281 cgaagagtat aaaatgatgg ggaaggctgg gcaactagtc tttttcgatg tttatagatt
    14341 agttaggcaa gtaagctagg ataggaatct ttttggcgct aagattttaa gaggagagac
    14401 tgcaagaagg gcttgattta tgataaaata gaagtctaag aagcgaatga agagagtaag
    14461 atgaaagaag ctataattga gtggaaggat ttctctttcc ggtatgaaac acaacaagaa
    14521 ccgaccttgc aagggataga cttgaccatt tacaagggag agaaagtctt aattgttgga
    14581 ccatctgggt caggtaaatc taccttgggt cagtgtttga atgggattat tcccaatatt
    14641 tacaagggtc agacatatgg agaatttttg ataaagggtc aagtagcctt tgatatgagc
    14701 atctatgata agtctcatct ggttagcaca gttttacagg atacagatgg gcagtttatt
    14761 ggcttgtctg tggcagaaga tttggcgttt gctctggaaa atgatgtgac agccctagat
    14821 gagatgaaag gtcgtgttta taaatgggct gaaaagctgg accttcttcc tttactggat
    14881 cagcgtcctc aggatttgtc aggtggacaa aagcagcgag tcagtctggc tggtgtcttg
    14941 attgatgaaa gtccgattct cttgtttgat gagccactcg ccaatctaga tcccaagtca
    15001 ggtcaggata ttatcgaatt gattgaccag attcataagg aagaggggac gacgactctg
    15061 attatcgagc accgtttgga ggacgttctg catcgccctg tggatcggat tgtcttgata
    15121 aacgatggtc gtatcctttt taatgggagc cctgaccagt tgcttgcgac tgatttattg
    15181 actcaaaatg gaattcgaga acccctttat ctaacgactc tccgtcaatt aggtgtggac
    15241 ttagtcaagg aagaacaatt agcagatctg gataacttgt ctatctcaaa agggcaaatc
    15301 cagttacaga cggaactggt aaaagaaacc ccagcattgc agtcactctt tatactagag
    15361 gaagtatctt tttcttataa tgatagaccg attttaaaat ccctacattt agatattaaa
    15421 aagggtgaaa agattgctat tgtcggaaaa aatggagcag ggaaatcaac tctagccaag
    15481 gctataagta gctttattca gacggaagga cgctatcttt gggaaaaaca ggatataaaa
    15541 ggcgattctg ttgcagagcg ggcggaacga gtaggctatg tgctacaaaa tcctaatcaa
    15601 atgatttcaa ccaatatgat ttttgatgag gtggctctag ggctccgttt gcgaggtgtg
    15661 aatgagaagg aaattgaaac gagagtctat gaaaccttga aaatctgtgg actttatgaa
    15721 ttccgtaatt ggcctatttc tgccctgtca tttggtcaga aaaaacgtgt caccattgct
    15781 tcaattttgg tcttaggagc tgaaattatt ctcctagatg aaccgactgc aggtcaagat
    15841 cagaagaact atactgagat tatggaattt ctcgaagagt tacatcaaaa agggcatacc
    15901 attgtcatga ttacccatga tatgcaattg atgctggatt attcagaccg ggtccttgtc
    15961 atggtggatg gagaattgat tgccgatact gttccagcca gtctgttgag cgatcctgag
    16021 ctgttagtaa aagccaatct aaaagaaacc tccatcttta acttggctaa gaaactagat
    16081 gtggatccac tggatttaac ggcattttac aaagaaagga gagagggatg caagctaaat
    16141 taatcggtta ccagcataga gatactgtga ttcatcgctt gtcaggagct gggaaacttc
    16201 tctttttcat tctggtatca ttggcggcca tgattagcta tgataccaga atgctcttgc
    16261 tgattgccat cttttcagtc tttctcctct atttatcaga aatccgcttt aaagatgtct
    16321 cctttgtagc cgtttttgcg acggtatttg ccgttttaaa cgttttgatg gtctatctct
    16381 tttctcccga gtatggggtt ggactttatg gagagagaag tgtgatttgg cagggaatcg
    16441 gtgtctacac tctaaccagc caggagctct tttatctgct aaatttggcc attaagtatc
    16501 tttgcaccat tcctctggct attatctttt tgatgacaac ccatcctagt cagtttgctt
    16561 ccagtttaaa tcaaattggt gtgccctata agattgctta ttctgtcagc ctgaccttgc
    16621 gctatattcc agatttgcag gaagaattct ttactatcaa gatgtctcag gaggcgcgtg
    16681 ggatggaatt atccaagaaa gcttctctta tgcaacgaat caaaggcaat ctgctcatta
    16741 ttacgccctt gatttttagc tcgctagaac gcattgatac cattgcgact gccatggagc
    16801 ttcgccgctt tgggaaagag aaaaaacgca catggtatag ttatcaggcc ttgaaaaaag
    16861 gagactatat taccttgctc ttggcaacct tgtttttagt agttagttta ctacttatct
    16921 tgcagaatca gggacgattt tacaaccctt ggaaatagct tgaaaaaatt gaaaaaatca
    16981 agtcatttct attgacaatg attctgaaag tgttatacta agaaagtagt ttcgctgatt
    17041 tacttcaaac ctgttgtgag gtaagttaac gatgccttaa ccacgctgtt tgctgagctt
    17101 gactccgggc agtgtggcta tttttttgca atggtgaaag gaagcaagtc atgacaaatc
    17161 acattgtatt atttgaacct caaattccac aaaatacggg caatatcgcg cgtacttgcg
    17221 ctgcgaccaa ttctcccctc cacatcatca agcctatggg ctttcctatt gatgaccgca
    17281 agatgaagcg ggctggattg gattactggg ataagctaga gatttatttt tacgagagtt
    17341 tggaagattt catatctcag atgaagggaa aactctatct gatttcgaaa ttcgcggaaa
    17401 aagtctattc tgaggtggat ttatcgactg acgaggacca ttattttctc tttggacgtg
    17461 aagacaaggg cttacctgaa gattttatgc gagaacatcc tgagaaggct ctccgtattc
    17521 ccatgaatga tgaacatgtc cgcagtctca atgtgtctaa taccgtctgc atgattgtct
    17581 atgaggctct tcgccagcag aactttgcag gtcttgagct cgtccatacc tatgaagtgg
    17641 ataaattgaa ataaaaaatg aaaatgaaca aaatgcttgc gcttgcaagc gttttttgtt
    17701 atgataaaag agtcttcagg gcagggtgtg attcccgacc ggcggtgact ttaactagga
    17761 aatgatcttt tccttttata ctttgttgac aagctttgcc taaccagaag ttatgcctac
    17821 agcttgtcgc ctagtctaaa agaaaaatct ctatttcttt ctctttaaag aagtccgcga
    17881 gcgcaagctg atgtggtgag attccacaac cgacagtata gtctggatgg gagaagacga
    17941 aagaatagct ttgtctgttt tgatagattt atagccggat tgtaaccact tgctttgttt
    18001 ttctttaata gagtaagagg gaacttttgg gatataaaaa gtgagaatag atagaggaat
    18061 cctttccaac ttcttctgat tttatagaaa attggaggaa cctgttatga caaacacacg
    18121 tcgactttcg accattgcaa ttctatcagc catctcattt gtgctgatgt actttgattt
    18181 tccgctttta ccagcggcat ctttcctcaa gatcgaattt agtatcttgc cagtccttgt
    18241 gggtctggtg gtcatggatt tgcctgctgc tctaggagtg ctcttgcttc gctcactctt
    18301 gaagttgctt cttaacagcc aaggagtgaa tacttacatt ggtttgccga tgaatatcgt
    18361 agctttggga gtttttgtca tcgtatttgc tttgatttgg aaaaaggaac ggacaaccct
    18421 tcgtttccta ctaggctctc tagctggaac tattggttta accttggcca tgttggttct
    18481 caactatgtt catgctgttc ctttgtacgc taagtttgct aactttgata ttggaaaaat
    18541 tttgggactt tccaactacc taatgaccat ggtattacct tttaacttga ttcaaggtgt
    18601 tatcttttcc gtttcattct ggttgttgta tgttctcttg aaaccaacct taaaacatta
    18661 tgagagataa acaaacattt ttaatgaagg gcagttttgc ccttttactt ttcgttattc
    18721 ttggctacat ggtcaaattt taccctgaaa cgctggtcgg ttttgaccaa tcgattcaga
    18781 ctgccattcg aggagactta ccagattatt tgactattct ttttcgatcc ctcacacgtc
    18841 tgattgatat tccagtgatt atcacttggg ttgtcattat agcttttgtc ttttatcgta
    18901 agcgatggaa gatagaaagt ttcttcatgc tgggaaatct ggctttggca ggtcttttaa
    18961 tcgtgacctt taaaaatatc taccagcgcc cacgaccagc tattttacat ctggtggagg
    19021 agaagggatt ttccttccca agtgggcatt ctctagctgt aacgctgatg gtagggactt
    19081 tgattgttat tcttagtcag cggattaaag atcaggtctg gagaaaaatc gtacaaatcg
    19141 tccttggcct ctacctagtc agtgtgctgg tatcaagagt ctatctggga gttcattacc
    19201 catcagacgt ccttgccagt ctctgtgtgg gcttgggagt cctgtttatc gagtttccct
    19261 tttatgacaa gctccgcttc caatggcgat ttaaaggcaa acagaagtga gtatcgaatt
    19321 cccttgagag aaagaaatga aagtcaatat agcagatctt catccgactc aactatattt
    19381 atcagaaaag aagttgcaag atattcagat gctttaccag tcggcagaaa caatccaagt
    19441 cgatccaatc agtattcttg cctttggaga ttgtttgctg attacagatg ggcatcacag
    19501 ggcttatcag gctttattgg caggtcggga tacgatttct gctgagtggg atagaggtgg
    19561 tggtgatgaa ctatatcatc tctatgcgca agcttgcgag gaaagaaaga tttactctgt
    19621 ttttgattta gaagatcgta tcctagctca agatgggtat gaagcaaaat ggtataactg
    19681 gtgtgatggt tttaatcaag cagcaactct tttgttgaaa aggtagtgca ttgtgttttg
    19741 ttccttatta taaggtcaat tattgtgagg aagcttttgc ttggtatcag gatgtgaact
    19801 tggtttacct cgtagatggg gtgaagcttc cttacagtca agcgaccttg gaagccatgt
    19861 attcctattt ggatcggcat ggtgagcttt tttggattga agtcaaggag aagggagaat
    19921 ggtttccaat tggggatatt acactatctc aggataatct ccccattgtg attgggaatt
    19981 ccgcttacca acatcgagga cttggaaaaa agattctaag tgctttgatt gaattggctc
    20041 gagtaaaagg atggaaagaa ttgagagtca aggaaatcta cacctacaat catgcttcta
    20101 ggaggtgttt caagtcgctt ggatttgtgg aaaatggagc aacagaaaaa ggaaggagtt
    20161 ttatattgga attagtctaa tcctgccact ttctccccct taacctttga ctattccgca
    20221 aaattatcgt aaaataaaga gtaaatgata aaatgaggtc agagtctgtt cgctctggcg
    20281 atagtagtat aaatgaggag aaacgctttg gaattagaag tatttgctgg gcaagaaaaa
    20341 agtgaactat ctatgattga ggtagcgcgt gctatcttgg aacttcgtgg tcgcgatcat
    20401 gagatgcatt ttagcgatct tgtaaacgaa attcaaaact accttggaac atcaaacagc
    20461 gatatccgcg aagctttgcc tttgttctac acagagttga actttgacgg tagcttcatc
    20521 tcacttgggg acaacaaatg gggtcttcgt tcatggtatg gtgtggacga aatcgacgaa
    20581 gaaatcatcg ctcttgaaga aaatgacgac gatgaagtag caccaaaagc taagaaaaaa
    20641 cgtgtcaatg cctttatgga tggtgattca gatgccattg actacaatgc agatgatcca
    20701 gaagacgaag atgcatacga agcagatcca gctctttcat acgatgatga aaatccagat
    20761 gatgaaaaaa atgaagtgga agcttatgat gcagaaatca acgaaatcgc tccagatgac
    20821 ttgggagaag atgtggatct caacgaagac gacgacgagt tttcagatga cgatgctgaa
    20881 accagcgagg aataagagtt agctattgac aattatccta tttttaggta tcatattgtt
    20941 tgggcacctc ttttagaggt cggggctccc tagttcttag ggagctattt ttgttttttc
    21001 aagaagttat cttcttgtat tttatactca atgaaaatca aagagcaagc taggaaacta
    21061 gccgtaggct gctcaaaaca ctattttgag gttgtagata agactgacaa agtcaggaac
    21121 acatatctac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag agtattagct
    21181 gtgaatctgg tgcagtcgtc ccagattatt cttattagta gggtcttgtt ttctatatcc
    21241 cctcgtagtt aacaagacct tgagcatttt agaaagagga atctatgtct acgaaatata
    21301 tttttgtaac tggtggtgtg gtatcgtcta ttgggaaagg gattgtggca gcgagtctag
    21361 gccgtctctt gaaaaatcgt ggtctcaaag taaccattca aaagtttgac ccttatatca
    21421 atattgatcc gggaaccatg agtccttacc agcacgggga agtttttgtg acagatgacg
    21481 gagctgagac agatttggac ttgggtcact atgaacgttt catcgatatc aatctcaaca
    21541 aatattccaa cgtgacaact gggaaaattt acagtgaagt tcttcgtaaa gaacgccgtg
    21601 gagaatacct tggggcaact gttcaagtca ttcctcatat cacagatgct ttgaaagaaa
    21661 aaatcaagcg tgccgctcta acgaccgact ctgatgtcat tatcacagag gttggtggaa
    21721 cagtaggaga tatcgagtcc ttgccattcc tagaggctct tcgtcagatg aaggcagatg
    21781 tgggtgcgga taatgtcatg tatatccata caaccttgct tccttacctc aaggctgctg
    21841 gtgaaatgaa aaccaaacca acccaacact ctgtcaaaga attgcgtggc ttgggaatcc
    21901 aaccaaatat gttggttatt cgtacagaag agccagctgg tcaaggaatt aaaaataaac
    21961 tggcccagtt ctgtgatgtg gcaccagaag ccgttatcga atcgttggat gttgaacacc
    22021 tttaccaaat tccactgaac ttgcaggcac aagggatgga ccaaattgtt tgtgatcatt
    22081 tgaaattaga cgcaccagca gcggatatga cagaatggtc agccatggtg gacaaggtca
    22141 tgaacctcaa gaaacaagtt aagatttccc ttgttggtaa gtatgtggag ttgcaagatg
    22201 cctatatctc agtggtcgaa gccttgaaac actctggcta tgtcaatgat gtagaagtta
    22261 aaatcaattg ggtcaatgcc aatgatgtga cagcagagaa tgtagcagaa ctcttgtctg
    22321 atgcggacgg gatcatcgta ccaggtggtt ttggtcaacg tggtacagaa gggaaaatcc
    22381 aagccatccg ctatgcgcgt gaaaatgatg ttccaatgtt gggagtctgc ttgggaatgc
    22441 agttgacatg tatcgagttt gctcgtcacg ttttaggtct tgaaggtgcc aattctgcag
    22501 agcttgcacc agaaacaaaa taccctatca ttgatatcat gcgtgatcag attgatattg
    22561 aggatatggg tggaaccctt cgtttgggac tttatccgtc taagttgaaa cgtggctcta
    22621 aggctgctgc tgcttatcac aatcaagaag tggtgcaacg ccgtcaccgt caccgttatg
    22681 agtttaataa tgccttccgt gagcagtttg aggcagcagg ttttgtcttt tcaggagttt
    22741 ctccagacaa tcgtttggta gaaatcgtgg aaattcctga aaataaattc tttgtagctt
    22801 gtcagtatca ccctgaactg tcaagccgtc caaaccgacc agaagaactc tacactgcct
    22861 ttgttactgc agcggttgag aacagcaatt agcaaaatca gaacctttga gaaaaatctc
    22921 agaggttttt tgcatacaat gatattgcag tatatctgag gtaggagtcc tctgtatgta
    22981 cctgctaccg ttgaaattaa tagcgactcc ctcttgccct gtgctagtga atggatttat
    23041 cagtatattg aaatgaaata aaatctgaac aaattaattc ggaaagccaa atcaatttct
    23101 agcaaagttt taggaactgg attgtatagt gaattgaaat aagatatgaa catc
//
LOCUS       DAVHIT010000027        23097 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVHIT010000027 DAVHIT010000000
VERSION     DAVHIT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.027"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(101..250)
                     /locus_tag="VVN16_000921"
                     /pseudo
     CDS             complement(101..250)
                     /locus_tag="VVN16_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(404..2035)
                     /gene="rlmD"
                     /locus_tag="VVN16_000922"
     CDS             complement(404..2035)
                     /gene="rlmD"
                     /locus_tag="VVN16_000922"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU5572340.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2109..3083)
                     /locus_tag="VVN16_000923"
     CDS             complement(2109..3083)
                     /locus_tag="VVN16_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU5572341.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3157..3771)
                     /gene="pmp23"
                     /locus_tag="VVN16_000924"
     CDS             complement(3157..3771)
                     /gene="pmp23"
                     /locus_tag="VVN16_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU5572342.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3771..4745)
                     /locus_tag="VVN16_000925"
     CDS             complement(3771..4745)
                     /locus_tag="VVN16_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU5572343.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4753..6009)
                     /locus_tag="VVN16_000926"
     CDS             complement(4753..6009)
                     /locus_tag="VVN16_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5572344.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(6071..6502)
                     /locus_tag="VVN16_000927"
     CDS             complement(6071..6502)
                     /locus_tag="VVN16_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572345.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6504..7106)
                     /locus_tag="VVN16_000928"
     CDS             complement(6504..7106)
                     /locus_tag="VVN16_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU5572346.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7090..7929)
                     /gene="prmC"
                     /locus_tag="VVN16_000929"
     CDS             complement(7090..7929)
                     /gene="prmC"
                     /locus_tag="VVN16_000929"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5572347.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7929..9008)
                     /gene="prfA"
                     /locus_tag="VVN16_000930"
     CDS             complement(7929..9008)
                     /gene="prfA"
                     /locus_tag="VVN16_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU5572348.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMLEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9005..9595)
                     /locus_tag="VVN16_000931"
     CDS             complement(9005..9595)
                     /locus_tag="VVN16_000931"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU5572349.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9719..9901
                     /locus_tag="VVN16_000932"
     CDS             9719..9901
                     /locus_tag="VVN16_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU5572350.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10037..11410
                     /gene="mnmE"
                     /locus_tag="VVN16_000933"
     CDS             10037..11410
                     /gene="mnmE"
                     /locus_tag="VVN16_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU5572351.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11457..12392)
                     /gene="dapA"
                     /locus_tag="VVN16_000934"
     CDS             complement(11457..12392)
                     /gene="dapA"
                     /locus_tag="VVN16_000934"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU5572352.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     YPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     IPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12448..13524)
                     /locus_tag="VVN16_000935"
     CDS             complement(12448..13524)
                     /locus_tag="VVN16_000935"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU5572353.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(13867..13939)
                     /locus_tag="VVN16_000936"
     tRNA            complement(13867..13939)
                     /locus_tag="VVN16_000936"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13904..13906),aa:Thr,seq:ggt)
     gene            complement(14039..14548)
                     /locus_tag="VVN16_000937"
     CDS             complement(14039..14548)
                     /locus_tag="VVN16_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU5572354.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14546..14642)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14671..15102
                     /locus_tag="VVN16_000938"
     CDS             14671..15102
                     /locus_tag="VVN16_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU5572355.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15184..15561
                     /gene="mscL"
                     /locus_tag="VVN16_000939"
     CDS             15184..15561
                     /gene="mscL"
                     /locus_tag="VVN16_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU5572356.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKTAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15603..16697)
                     /gene="hemH"
                     /locus_tag="VVN16_000940"
     CDS             complement(15603..16697)
                     /gene="hemH"
                     /locus_tag="VVN16_000940"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU5572357.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESQQIRFLVIKDWYQQQSLLDFWTDEIRKILRNEVGEE
                     SFKVIFSAHSVPIFALDYGDPYIDQIFDNSRLIAEQLGLMTDQYTNTWQSESDIGIPW
                     IKPDVLEYLREQKQHPEHYIFVPISFISEHIEVLFDNDVECYDLCQELGVTYHRPPMP
                     NTDSRLIDALVATVRANEDKEFKAFLPEEETFDELAPSATTKDIMKETDDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(16775..17561)
                     /gene="srtB"
                     /locus_tag="VVN16_000941"
                     /pseudo
     CDS             complement(16775..17561)
                     /gene="srtB"
                     /locus_tag="VVN16_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            complement(17676..18674)
                     /gene="srtG1"
                     /locus_tag="VVN16_000942"
     CDS             complement(17676..18674)
                     /gene="srtG1"
                     /locus_tag="VVN16_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HEU5572358.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(18692..19888)
                     /gene="pitB"
                     /locus_tag="VVN16_000943"
     CDS             complement(18692..19888)
                     /gene="pitB"
                     /locus_tag="VVN16_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HEU5572359.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(19933..20484)
                     /gene="sipA"
                     /locus_tag="VVN16_000944"
     CDS             complement(19933..20484)
                     /gene="sipA"
                     /locus_tag="VVN16_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HEU5572360.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLATL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFSMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(20484..23081)
                     /gene="pitA"
                     /locus_tag="VVN16_000945"
                     /pseudo
     CDS             complement(20484..23081)
                     /gene="pitA"
                     /locus_tag="VVN16_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516426.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus tip adhesin PitA"
BASE COUNT         6581 a         4883 c         4386 g         7247 t
ORIGIN      
        1 accaactatt tattttttga gttttaaact ttggtagaag tcttgaactc cttatattta
       61 cataactatt tgcctctata actattatga cacgaaacca acaagcaagt tcaagcacct
      121 ccatatcttt attcaaatga ggactaagat attcacaaac tttatcataa acctctttat
      181 cttttttcat aaaaggggca tacaacttag caaatttatc ctaaaatttt ttgttgcctt
      241 tatcaatcat ttcaacactc ctttaaatat aaaaatgatc ctatcaatct ttatattgag
      301 cctattttct aattgatacc ttgcaagaaa ggaataatag ctgttcctat accaagtagg
      361 aatgtaataa atgcagaaag tcatatactg tcattctttt ctattaaatc attttgaagt
      421 gtctcaaagc atccatgatg gcttcttctt tttcaggtgt acactgtgga ataatttgtt
      481 tatccttttt agacttgttg taatgttctc gtaattctat tccacatttc ttttttatct
      541 gtgcaatata taatgtcgaa acttttaatt caaatttatt ccaaacatat tctttgattt
      601 gagcatatgt tgctttgctc tccgcacttg tcaaattcat ctcatccagc tcaatttcaa
      661 cacttatgtg cttatcgaca tcgagtttgg acaaaagtgc taccgtctcg acgtgatgcg
      721 tttgaggaaa tagatccacc ggctggactt tcttcaattc atatcccaat tcttggtaga
      781 gtttgatatc acgcgccatg gttgcgacat tgcaggagat ataggcgatg cgatcggctc
      841 ctgtttgggc gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga ggatcaacca
      901 agataacggt tggttgaatc ccatctttaa gccaattctt catagcattt tcagctgtgt
      961 cacagacata gtgggcgttt gaaatattgt tcagctgagc atttttttta ctattctcaa
     1021 ccgcttctgg aatcagttca acaccgtaga cttctttgac atgcttggcg actgataaac
     1081 caatggttcc aataccagaa taagcatcaa taaccacgtc atcttttttt aactctgcaa
     1141 agtcaatggc tgtttgatag agtttctccg ccatttcagt attgacttgg taaaaggctg
     1201 ggccagcgat ttggaagtca tttcccaaca tctggtccgt aatatagtct tgaccataaa
     1261 gagtgcgcca ctccttacca aaaatcgcat tggtattctg gtcgttgata ttttgcatga
     1321 cagacacaat ctctgggaac tgcttgataa cttgttcaat caattggtca acacgaaaaa
     1381 cttttggacg agttgtcacc aaaacgacca tgatttgtcc tgaatagtga ccacgacgca
     1441 ccacaagatt ccgaatcaat ccagactgtt ccttttcgtc ataaggtttt aaatcaaaac
     1501 gacggagcag gtctcgaaga gctactacga cttggtcaat gacaggatcc tggataaaga
     1561 aatcttcaag gggcatgagg tcgtgcgaat tcttacggaa aaatcctgtt tccaagacac
     1621 cattcactcg acgaacgggc acctgcgcct tattgcgata cttgactgga tgttccatac
     1681 caagcgtttc agcaacttct acatctgcaa ttccagcaat cttgtagaga ctgtccttga
     1741 cttgcttggt tttaaacttg agctgttctg gataagaaag gtgtcctaaa tccgcgattc
     1801 ctgaacgcag gtaagccaaa tctagatctt gattacggtg tggtgactgg acaaggtatt
     1861 tttcaacttt tccaaagcca atctttttat tgaccttgag gacacgcatg agaatttttt
     1921 cactcggtaa agcattctct acaaaaaaga ccaaaccatc taccttggca actcctgccc
     1981 cttcatgggt caagtcaaca atttcaactt ctacaatatc atttttcttt aacattcttt
     2041 tctctttcta ttggccgaca aaaaagacgg caacgttacg gttaccatcc attataccat
     2101 gaaatttctt aagaaccaaa actcttgaag aagttgacaa gggcttgcca gagggagctt
     2161 aagaagttac caccgtcctc tagcgcctta tccgcatcaa agttaaggtt gatattttta
     2221 aaactgtcgc cagcttgtga tacgatgctt tgtttaaggt catttagggt tttagtgaaa
     2281 tctgcattgc tgaggatatc actctttgag agattcaagg caaaattgat gatgatattg
     2341 atctggtttc ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc ttcaacaatc
     2401 ttgcggatat cttcttctgt cagatttccc ttgctttctt tagctttggc gagtcctgac
     2461 ttgatatcag ctagggcaac gtttaattta ttagcatcat agcctgattt gtccttgttt
     2521 tcagcattga tatctgacaa agcttttagc tcttcttgag ccaaatcttt attagcttgt
     2581 ggcaccttgg ctccattagc ctctagcgaa tagtaaatcc ctgctaaagc actttctcct
     2641 gtaactggaa taggggctgc tacagtgatt ttggcatgtt ccatacccag cgttactgct
     2701 gcgtttcggt acatatcctg agtcacctta gtgatatttt ctggtgtttc aatcttgacc
     2761 tcaagtggcg atttgtcacc tagcttttga atcttggctg atgaatacaa ctgtaagcta
     2821 gagtcattgg ccacattcat gattttagaa taaacatcag gtgtcatggt cttgagttct
     2881 ttggtatctg ttgaggcatt gtagcccagt tttttaagag tttgattttt ttggtcttca
     2941 gatagggagg aacctaggac atattcaggt tggacatagg tttcatcgat aactttttga
     3001 acatctgttg ctgcatggac gctattcata gctgttactg cccacaagac cgcagcacta
     3061 gtcagaaaga gtttctttct catagggaat ttcctccttt acttctttat agtaatatat
     3121 ctatcttaaa gaaaacttat aacaaaaaca cctggtctag ccagatgttg aaaagagagt
     3181 gaaacatttg atgatgtaaa ggttaagtcg tacctgtcta gaataataat agtttcctcc
     3241 atttacatag agttcagcac cgtgaaaaat ggaaatgggg tgaatataac tataagtctt
     3301 tccagtcgta ttaccaagca agggggcaac agtctcacga gagtactgtt tggctagagc
     3361 caaggtattt tccttgccat tttgggcgat aaaatcgata taggcaggtc caaaattata
     3421 ggcttgaaca gctgtccaga tatctacacc cttcttctgc gccagataga gattgcctgt
     3481 cagagtttga atgccttgcc gaatgctaga ggcattatca ttgatggtgt tggtggaacc
     3541 acttgcagac tcactagact gcataacatc gccttctttt ccttttgttt cagtataaat
     3601 catagcaagc acaagctctt cgtttgctgg ggtgtctttt tcactcaata tttctcgcac
     3661 catgggttga taggtcatga cttgtttgac atcttgatga acgcggtaag ctttatagcc
     3721 agcaaaaagg aagactgcta gtacaagcac tcttcgaatt cgtttaaaca ttatttactt
     3781 tggatatcct cgatattttt gattaagata gagtaggttc cattttcgtt ttggataaac
     3841 tcaacagact cggcgtcttg atagacgtta ttgggaacga tgagctcaat tccatttgat
     3901 aaggagagtt tttggttttc aaatttcttt aattggcgac tggcatcaat ttcatcaaat
     3961 tgaacaggtt ctggtacggc ttctttgact tggtcaataa agctcaaacg agccgtcaga
     4021 ttgttgtcaa aaaggtcatt agccaatttc tcaggtgaca attcattgct ttcttctagg
     4081 ttgttgaaaa tagctgattt gaccttggat tgaaattgaa aatcatctgt gttaaaagat
     4141 tcagcaattc tctgggctgt tttttccagt tccttgatag attttttagg agaaatctta
     4201 ggagcgacag caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt gtacttgatt
     4261 cgtttttcaa tcaggtgata cttgctactc tgaagattga ccaccaaggc ctcatcagct
     4321 cctgttccaa atccaggcag gttattctga gttagcttga ttggattatc aacttctcct
     4381 ccgaggtggg tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg ttctacacct
     4441 tctttagaaa attgaacaaa aatcaagtca ttggtcttga gattttcaga aatgctaaac
     4501 tcctctttcc agagattagc cagcgttact gatgtctcca acaaatcgtc tgtaatatga
     4561 ttgaagaagg gattttcttc ttcgaaaatc ccagtcttgg cttcatctga atacacatgt
     4621 tcaatttttt tacgtaggta ttcttcgatt tttggagtaa tattgagaaa cttatctgct
     4681 aagaacagct cggtatcatc cggactgaac tggtgaataa tggctttctt aatataaata
     4741 tccataaaag ttttagtcct cgtataatgg gaaggcatct gtcaattctt tgactgcact
     4801 tctcacttct tctaatacag cctcattttc tgaattctta agggttttaa tgatgagttc
     4861 agccactttg cgactttctt cttcaccaaa tccacgtgca gtaatagctg ctgctccgat
     4921 acgaatccca cttgtcttga atggcgacaa ggtttcgtaa gggattgaat ttttatttag
     4981 ggtaatattg acttcatcca gcaagttttg agcaactttt ccgttttcta caaccttagt
     5041 cacatcaaca aggaagagat ggttttcagt tccacctgaa ataatacgga aatcagggtc
     5101 ttgcaagaag acatctgcca tagccttgct gttcttaatt acattggcag catattcctt
     5161 gaaggctgga tccaaaactt ctttgaagga aactgcctta gccgccacaa catgctctaa
     5221 aggaccgccc tgaatacctg ggaaaatagc tgaattgatt tttttagcaa gttcttcgtc
     5281 attggtcaaa atcaaaccac cacgaggtcc acgaagggtt ttgtgggtcg ttgttgttgt
     5341 gatatgagcg tatggaactg ggcttggatg aaggccagcc gcaaccaaac cagcgatatg
     5401 ggccatgtcc accatgagct tcgcaccgac agcatctgcg atttcacgga attttgaaaa
     5461 atcgataatt tgagaatatg ctgaagcacc agctacaatc agttttggtt ttacttcttg
     5521 ggcttgtttc aagatagcat caaagtctaa gagttccgtt tcaggatcaa cactataaga
     5581 aacaaagttg taggtttgac cagagaagct aacaggagcc ccatgagtca aatgaccacc
     5641 tgatgccaaa tccattccca taaccgtatc acctggctca atcaaggaca tgtaagccgc
     5701 acagttagct tggcttcctg aatgtggttg gacattggca aatttagcac cgaaaatttc
     5761 ttttgcgcgt tcaatagcaa gagtctctac aacgtctact acatcagttc caccataata
     5821 acggcgtcct gggtaaccct cggcatattt atttgtcaag atagaccctt gagctgccat
     5881 aacagccttg gaaactacgt tttccgaagc aattaactcg atattatttt gttggcgttc
     5941 ttcttctttg gcaatagcat tccagagatc agcatcatat gctttaaaat catctttgtc
     6001 aaaaatcata ggtcttctcc tttattgtgt gactagtcca ttagtttgat tttacaataa
     6061 gaaaatcaaa ctaaaagatg cgaataaacc gtttctgcat tttatcacaa gtatagccaa
     6121 ctttttcata aaatgcatga gcacccagac gatgattggc agaatttaag cggataaacc
     6181 cataaccaca tctttttgct tcttcttcca acccttgtag taaactttta ccaatacctt
     6241 gaccttgcgc ttgaggtgaa actgctaaag ctaagatatt aaatcctgct ttggaataga
     6301 gtgattcgta aacttcagcg tggacatatc caagtaagac atgattagct gcatcctcat
     6361 agccaagtag gaaatgatgg gaatcctgag acagtctagc tagttggcta gccgtttcct
     6421 ctggactaaa agtataaccc aaagcctctt ggttgatgtc acatatcgct ttcacatctg
     6481 tttcttgcaa atctcttagc atttcaagcc tcctcaaaag aaatctctgg caaccgagca
     6541 agaatatctt ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc tccagacaaa
     6601 tccacaatag ttgaatcctg tccagttaga aaagcatcgt cttccagacc cagaacctct
     6661 tggtcaaaat ccttcagaat ttgttcaaag gttacaccac ttgcctgacc tgagatattg
     6721 gcagacggcc caatcaaggg acctgtctct cgaattaaat ccagtgtgat agggtgactg
     6781 ggcatccgaa atccaatagt tgcaaggtca gaatttaccc aatagggaac tcggtcattg
     6841 gcttcgagaa taatggtcaa gggacctggc aaaaaggtct ctacaagttt ttgtagataa
     6901 gttggctgat tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt gagattgagt
     6961 gccttatctc taggacgacg tttgagttgg taaacatggt caactgcttt ttcatctaag
     7021 gccttggcaa aaagaccata aacagtctct gtaggtagaa cgacagctcc accattttcc
     7081 aactcttgtc taatcctgtc catcatcaac cacaaccatc ctattttgac caaattggtc
     7141 tttgagtgtt cgaacttttt tttcaggaag atgttttcta aaaagttcgg gaacactttg
     7201 accttgcttg tatccaattt caaggtaaat cttaccacca tccttgagat agtcttttgc
     7261 atcttccgca attctacagt aaatagctag gccatcctcg tctgcaaaga gagctagatg
     7321 aggctccgaa tgcaaaacat tcaagcctac ctctgactca tcttcacgag agatataggg
     7381 tggattggat acaattatat catatttttc agaaatttct gcaaaacaat cagatttttt
     7441 aaaaaatata ttaagatttt gatcgctagc attctctgat gcaagctcta aggcctcttg
     7501 agaaacatct gctgctgtca ctgaccaatc tggtctattt tttgctagag cgagagcaat
     7561 agctccacta cctgttccaa tatccagaac tgagagattc gtctcaagat tttcagttag
     7621 aataagctcc actaactcct ctgtttctgg acgaggaatc aaaacccgtt catccacctt
     7681 taaatgcatt ccataaaaat ctgcctgtcc aatgatgtac tgagctggct tgtgagctgc
     7741 tagttgctgg taaatatctt ctacaaattg tttttcttcc tctgttgtca cctcctgctg
     7801 gagggcaaaa ataaagtctg taaaagatag atttttcaga ctacgataga caaaagagag
     7861 gctttccgct tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa ataattgagc
     7921 taatttcatt atttgtttaa ttcttctagt ttttgtgttt ggtcataaag caccaaggca
     7981 tccacaactt cgtccaattt accagacaaa atcgtatcta gtttttggag ggtcaagccg
     8041 atacggtggt ctgtgacacg gttttgtggg aagttataag ttcggatccg ttctgaacgg
     8101 tcaccagtac cgattgtcga cttacgctca gcgtcttgtt catcctgagc aatctgagca
     8161 aagtggtcag cgacgcgtgc acggataatc ttcatagcct tctcgcggtt cttctgctgg
     8221 gtacgttctt cctgcatctc aaccttgata ttggttggca agtgaacgat acgaacggca
     8281 gtcgcaacct tattgacgtt ctgtccacca gcaccagagg cgtgatagat gtcgacacga
     8341 aggtcttttg gatcaatgtc gtattcaacc tcttcaactt ctagcataac aagaactgtc
     8401 gctgtcgaag tatgaacacg gccttggctt tctgtcacag gaacacgttg cacacggtgg
     8461 gcacctgatt catacttaag cttagagtat acagactgac ctgaaaccat agcaaccact
     8521 tctttaaaac caccgacacc attcatagag gcttccatga cttcaaagcg ccaaccttgg
     8581 gcttccgcat acttttggta catagttagc aaatctccag cgaaaagtgc cgcttcgtct
     8641 ccaccagctg ctccacggat ttcaaggatg atattcttgt catcgtttgg atcctttgga
     8701 aggagcaaaa ttttcagttt ttcttcatat tcttcttttt cagccttggc atctttgagt
     8761 tcttgcttgg ccatttcttc caagtccgca tctccgcctg attccttaat catctcttcg
     8821 gcatcgacga tattttgaag gacttgttta tactcacggt aggctattac ggtgtcacga
     8881 ttggaagctt cttcttttga aagctccata aaacgcttgg tgtctgaaac gacatcaggg
     8941 tcactcagca attctcctag ttcttcataa cggtcttcta cagcttgtag ttgatcatag
     9001 atgttcattt ttcttcattc tccttattga tttcaggggc aaaataatgt ttacggcaaa
     9061 ccgagatata ggtttcatta ccaccaatct ggatctgttc tccatcgtaa acgggcagtc
     9121 catcctgtgt tcgcaacacc atggtcgcct ttttcttgca atactgacat atggtcttga
     9181 tttcgtcaat cttgtctgct aaaagcaaga gatatttgga accttcgaac aattcattgc
     9241 gaaagtcatt tttcaagcca aaagccatga cgggtatgtc taactcgtcc acaacacgag
     9301 ctaggtcgta aacatggtgg cgtttgagaa actgggcttc atcgaccaaa acacagtaag
     9361 gtttttctgg taggtctcgg atatagccaa agatatccgt tgtttcctca atcgcaaggg
     9421 cagggcgttt catgccaatt cgactcgaca catagccaac accgtcacgc gtatccagag
     9481 ccgaagtcat aatgacaaca ccttttcctt gctcctcgta gttataggcc actttgagaa
     9541 tctcaatcgt tttaccagag ttcatggtcc cataacgata atacaactgt gccatgtttc
     9601 ttgcttcacg tccatttcta aatttttgct acattctagt atatcataat tttcttaagc
     9661 tttaaacggc aaaatgtggt aaaatagaag aaatcaaaaa ctagtggagg aagctattat
     9721 gccatttgta cgcatcgatt tatttgaagg acgcacgctc gagcaaaaga aagctcttgc
     9781 taaggaagta acggaagcgg ttgttcgcaa cactggagcc cctcaatctg ctgttcatgt
     9841 catcatcaac gacatgccag aaggaactta tttcccacag ggggaaatgc gcaccaaata
     9901 agctagctta agcagaattg cttaggcttt ttcaatctcc aagtagcacc cattgaagaa
     9961 atagtctaaa tttgttacaa tttgaaaaga gacttggaaa attccaagaa aagagcaatt
    10021 aattaaagga aatattatga ttacacgtga atttgatacc atcgctgcta tctctactcc
    10081 actaggtgaa ggagctattg gtattgtccg cctgagcgga acagacagtt ttgctattgc
    10141 gcaaaagatt tttaaaggaa aagacttgaa caaggttgcc agccacactc tcaactacgg
    10201 tcacattatt gatcctctga ctggtaaagt catggacgag gttatggttg gggctatgaa
    10261 gtctccaaag accttcactc gtgaggatat tatcgagatt aacacccacg gtggtattgc
    10321 ggtgaccaat gagattctcc agctagctat ccgtgaagga gctcggttgg cagaacctgg
    10381 tgaatttacc aaacgtgcct ttttaaacgg tcgcgtagat ttgacacagg ccgaggcggt
    10441 gatggatatc atccgagcca agacagacaa ggccatgaac atcgcggtca aacaacttga
    10501 tggttccctt tctgatctta tcaataatat ccgtcaagaa atccttaaca cacttgccca
    10561 agttgaggtc aatatcgact atcctgagta tgacgatgtt gaggaagcca ctactgctgt
    10621 tgtccgagag aagacaatgg agtttgagca attactaacc aagctcctta ggacagcacg
    10681 tcgtggtaaa atccttcgtg aaggaatttc aacggctatc attggacgtc ccaacgttgg
    10741 gaaatcaagc cttctcaaca acctcttgcg tgaggacaag gctatcgtaa cagatatcgc
    10801 tgggacaaca cgagatgtca tcgaagagta cgtcaacatc aatggtgtac ctctcaaatt
    10861 gattgataca gccggtattc gtgaaacaga tgacatagtg gaacaaatcg gtgttgagcg
    10921 ttcgaaaaaa gccctcaagg aagccgactt ggttctacta gtgctaaatg ccagtgaacc
    10981 actgactgcg caagacagac aacttcttga aattagccaa gataccaatc gcattattct
    11041 acttaataaa accgacctgc cagaaacgat tgaaacttcg aaactacctg aagacgttat
    11101 ccgtatttca gtccttaaaa accaaaacat cgataagatt gaagagcgaa tcaacaacct
    11161 cttctttgaa aatgctggtt tggttgagca agatgctacc tacttgtcaa acgcccgtca
    11221 catttccttg attgagaagg ccgttgaaag cctacaagct gttaaccaag gtcttgaact
    11281 agggatgcca gttgacttgc ttcaagttga cttgacccgt acttgggaaa ttctaggaga
    11341 aatcactgga gatgctgctc cagatgaact catcacccaa ctctttagcc aattctgttt
    11401 aggaaaataa gaaaaatcca tgatccttca ttcggtcatg gattttattg tctttattag
    11461 taatctggtc ttaagacccc tgttacagtt gccttagttg cttcgtagtc gccatctacg
    11521 acaaccttga taatgcgttt ggcatcttct tctggtgctg gaataagagg tagacgagtg
    11581 ggtccagctt caaatcccat atagttaaga actgccttaa ctggagcagg acttggataa
    11641 gagaagagag cattaacctt aggaatgaat ttacgctgaa ttgctgcggc tttcttcata
    11701 tcgctttctg caatggcagt aaacatctcg tgcatttcat ccccatttgt atgagaggca
    11761 acagaaataa ccccatccgc cccaaggttc atggcatgga aagcatctcc atcctcacct
    11821 gtataaatca agaactcttc aggcttgtgc tcaatcaagt aagccatatt agccaagcta
    11881 gtacattctt tgacaccgat aatatttgga tagtcagcca agcgaagcat ggtttctgga
    11941 gtcaattcga caactacacg ccctggaatg ttatagataa taattggtag gtcagaagca
    12001 tctgcaatag ccttaaagtg ctgatacatc ccttcttgag aaggtttgtt gtagtaagga
    12061 acaatagcaa gcccagctgc gaaaccacca aattccgcta cttctttgac aaactcaata
    12121 gagtcacgcg tatcattagt acctacaccc gcaatcaaag gaacgcgtcc attgacaacc
    12181 ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg tcaaagttgg actctcagca
    12241 gtcgttcctg cgagaagaat tccatccgta tgatgggcca ataaatgctc aatcaaggct
    12301 ggaatagcat caaagttaat ggaaccatcc tcatggaagg gggtaataaa ggctgtaatg
    12361 attttacatt cttttaaatc ttgataagac acgaaaacac ctctctattt caaagaagga
    12421 gttcatctga actcctaaac gatatgacta ttttaattca aatttcaatt cggctgttgg
    12481 acgaaccaat ccacgttcat gaagagtttc agcaatctga actgagttcc aagcagcacc
    12541 tttgagaagg ttatctgaaa caacccacat gtgaattcct ttttctgcat ccaagtcttt
    12601 acggatacga ccaacaaagg tatcacgcga accaactgca ttgatggctt gaggatagat
    12661 ttgatgagct acatcatctt caagaacagc acctgggaag gctgcgatag ctgcttttac
    12721 ttcttcgatt ggagccactt cttttgtttc gatataaaca gactcagagt gagctgacaa
    12781 gactggaata cgcacacatg ttgcagatac tgcaatgcta tcatcttcca taattttctt
    12841 agtttccttg gtcatcttca tctcttcgta agtgtaatca ttgtcagtga agacatcgat
    12901 ttgtggaaga gcattgaagg cgataggata atgtttcttg tcaccgcctg aaggtaagat
    12961 ttccgcatgc aaatcacgtg gtttcactcc atcattcaag acctcacgaa gttcacgttg
    13021 tgtctcaaga attgctccca taccagcacc tgaaacggct tgataagttg aaacaatgat
    13081 acggtccaag ccccattttt ggcgaaccgg ctcaagagcc accatcattt ggattgttga
    13141 acagttaggg caggcaatga ttccgttgtg ggcatcaagt gcatgagcat tgacctctgg
    13201 aacaaccaaa ggaacatctg gattttgacg gaaataagat gtattatcca ctactaccac
    13261 gccagctttt actgcgtatg gtgcatactt agctgatgtc gaaccacctg ctgaaaagag
    13321 agcaatatca actccttcaa aagctgtttc agtcgtttct tcaatcgtaa tatcttgatc
    13381 tttaaatttc aatgacttac ctgctgaacg tgcagaagca aggtaacgga ttttatcgat
    13441 tggaagtgtt gattcttcca acatttttat catctgagca ccgacagcac ctgtcgcgcc
    13501 gactacagca actgtatatc ccataaataa cctctttcgg aattttctaa aaatttctat
    13561 aatagaagta ttatactact ttttccatga attgcaaagc tttttcatca ttttttggaa
    13621 aataaaaatc cccaatcgct agactaggga ttaagaggca tcttcatgtg atgagcaggt
    13681 tcacacaact catcaaggtc cgctcctgcg ttatgacctc cttatgctca atagtagtcc
    13741 gaagactacc tatcgactca tcgcatattc tatattacta gagatacttg attttgtcaa
    13801 taagaaattt tttcgatcga gtataacaaa aaactctcgt attcaagagt ttccctatac
    13861 atatcatgct aattgccggg attgaaccgg cgacctcatc cttaccatgg atgcgctctg
    13921 ccaactgagc taaatcagct tacttaagaa gtatactata ttcagaaaaa cttgtcaagt
    13981 ttccttagaa attttccata ggaaaaagcc aggtaagaac tacctagctt atcttcttct
    14041 atttacttaa atcaattcga cgaccaattt taccaataat aatcgctcca ataatcaatg
    14101 aagcaaattc accgattcct gttgtaaacc aagtaaggaa gaatggtaat tctgctacaa
    14161 tatttaactc tgcagcgata gtaaacatgg aaattgaaaa gaggattgaa aagaagaaat
    14221 gatcttttcg aatcaatccg ttaaagaggt aatccttgct atattttgca aaaagccaaa
    14281 cacctaaact gaggaatact aaggttgaac caccaccaac aaagacatct agcagtccga
    14341 agctaaagaa attagcaatc atacaaccga tggtaactcc gatgatgtac ttaggattgt
    14401 aaaaagccat aaagttcatc atttctgaaa tacgaaactg ataagcacca tagctgatgg
    14461 catttagggg cggtgtgaca gtcaaaacga cgtagatagc agcgacgatt gcaatatctg
    14521 caacatcacg aatagttaat tttttcatct tttctccttt ggcggttatc cgcgtgtaat
    14581 atgcttggtg aaagaagcta agcaccaagg gttgattcat tcaaccttac tagtatagca
    14641 caatagcctg ctttatgcta tacttaaagc atgaaaaaac ctattcaaaa attttttaac
    14701 aatgaaatct tagcctatct cttttttggc cttgcgacaa ctctagtttc gattctctca
    14761 cgactagtca tttatcaact aagtcatcag gaactccttg ctacggcctt ggcaaatatt
    14821 atcggtatct tatttgcctt tatcacaaat gatacgattg tatttaagca aaaaaagaaa
    14881 aatagactta tccgtttagt gaaattttct cttgctcgcc tttctacttt tctgttagac
    14941 ttgtttttaa cttttctctt tgtaacccaa tttcctcaca ttatagggca attcgtaact
    15001 gaaaacattg ataagataaa tgctattgaa acaataattg cacaaatatt cattattatc
    15061 atcaattata tcttgagcaa ggtctatatt tttagaaaat aatgctccat ttatgagcct
    15121 tttaattgcc ttctataatg atttaggata caatgaaaca cgaaacattg aaaggaaaac
    15181 agtatgttaa aaaatctaaa atcgttcttg cttcgaggaa atgttattga ccttgctgtc
    15241 ggtgttgtca ttgcctctgc ttttggtgct atcgttactt cacttgtaaa cgacattatc
    15301 actcctctta ttttaaatcc cgctttgaaa actgctaaag tcgaacgtat cgctcaactt
    15361 tcttggcatg gagtcggcta tggtaacttt ttaagtgcta ttatcaattt tatctttgtg
    15421 ggtaccgccc tcttctttat tatcaagggc attgaaaaag cacagaaact gactggcata
    15481 aaggaagaaa aaactgccga aaaaaaacca accgaattgg aagtccttca agaaataaaa
    15541 gctctccttg agaaaaaata atcgattaaa aggatctagc gcaaagctaa atcctttttt
    15601 ctttactctt tgggtaactt gcctgctttt tcaagcattt tcttgatcag ataaggcatc
    15661 ttaacatttt cacgaccttt tttctcaatg agttttttga caaattctgg catctgaagg
    15721 tcgtctgtct ctttcatgat gtccttggta gttgccgaag gcgctaactc atcaaaggtt
    15781 tcttcttctg ggagaaaagc tttaaattct ttgtcttcgt tggctcttac agtagcaact
    15841 aaagcgtcaa ttaaacggct atccgtattg ggcatcggtg gacgatggta agtgacacct
    15901 aattcttgac acaaatcata acattccaca tcattatcga acaagacttc gatgtgctca
    15961 ctgataaagc taatcggaac aaaaatataa tgttctggat gttgcttttg ttctcgtaaa
    16021 tattctaaga catctggctt gatccaaggg attccaatat cgctttcgct ctgccaagta
    16081 ttggtatact ggtctgtcat taggcctagt tgttcagcaa tgagcctgct attatcgaaa
    16141 atctgatcga tataaggatc tccatagtcc aaggcaaaaa tgggaacgct atgggctgaa
    16201 aagattacct tgaaggattc ttctcccacc tcatttcgta aaatttttct aatctcatct
    16261 gtccagaagt ccagcaaaga ctgttgttga taccagtcct taataactaa aaatcggatc
    16321 tgctggcttt ccagaaactt ttcatacccc atgactgagt aaaaagaata atgaggttcc
    16381 aaaatcaagc agatacattc ttcaacacca tcagtttcca tctgcttgat cacgtctgga
    16441 ataaagggac gagaaaattt gttggcaaag tagataccgt actcctcccc taaacgttct
    16501 ttgactaaat tgacctcctc acgtgtaatc cgctgcaaag gcgtcccacc gatacgaata
    16561 tagttgtcat agagagtctg gatttcgtgg tcttggggtc taactccacg acgaatgttt
    16621 gtaaagaatt ctgctactcc ttcaaaacta atctcctctg gagatccaaa ggtcatcatt
    16681 aatatggctt ttttcatgtt atcatcctta tgatattatt ttctctatcc attctatcat
    16741 gaaagaaaaa aatagtaaac gtttcctacg tctactattt cttttgctct tttgtttttt
    16801 tatctcgtct tataattagg atgataagga caagaagtgg gaattccgtg atcgaaatac
    16861 cgtgttccta ctgcttcccc actttcatcc tgtgctactt tcaccggtgc atccgttatt
    16921 ttagctaaaa ggatatgtcg tccattagtt atgttaagga aacaggtgct taataaaaca
    16981 atcttgtccg ttgaggtcaa ggaaacatct cgcttgtact tggctttact tagcagatat
    17041 tgatagtaag cctggtgttc ctcatccttg gaactcaagg ttcgataaat ctccgtgtca
    17101 taggcatcca cttctaaaat cccaaaaatt tcaagaccac gttctcgacc attgtagtag
    17161 atagaaccgt agcgatgctg gtcgaagaat tccttatcag caaatttctt aatctcaccg
    17221 aacatagccc ctgaagccat agagtgtccg tagatgatcg tattaaaatc agtaaagttg
    17281 ggattaaagc gatagtcgag aaaaattgat cccgttatcg tataatcgcc aaaggcattg
    17341 cggtcaaggt atttgacatt atcctgactt tgtagaagcg gatagtctac cttggtccca
    17401 tcaaggctaa tccaaccgca aacatctttg ttcaagtgct gtagggtttc aaaacctgtg
    17461 ctcgaagcat ccttttcaat aggattatag tgatcatagt cacttgcttc tactactctc
    17521 tgccaactaa aaagagctac aaaaacacag aagaatacca gaaatacgtt tttaaatcgc
    17581 gctcttttga accgtcatca gcactccttt ctatgcgaga tttcttaaaa ggaaaggagg
    17641 cgggacaaaa ccgcctcttt tctattcacc cttagtcagt aaaagggtct gcttcttgca
    17701 ttttttttac tctctcttct acgacgtagg atcaagtaga ggacaaggag taagaaaatc
    17761 aagagcaaga acaatacaaa caagataagg taccaaatcc atagtgggat tgatgagagg
    17821 aaacgtccaa gagacgagtc atcaaagaca ctgtatggaa atggttttga tttttttgta
    17881 tggaaagtat ttttagggac agtgtctgta atctttgcga ctacgatatg acgaccattg
    17941 gtcacatcga gaaaacaggt actgagtagg atgatacgat cactcggtga gagtgagata
    18001 tcccgcttgt gcatggcgac tgagagcagg tggtctagat aggcctgctg gcggtcctca
    18061 ccctgtattc ctggatcata gatgttaaag tcataggcat caacctccaa catctcaaag
    18121 atctcgagcc ctttttccac accattgtag tatatactac cgtaacgatg ctggtcaaaa
    18181 aattcctgat cagcaaactt agccacatca ccaaacatga ccccattttc tacgtggtgc
    18241 ccgtagataa tggtattaaa gtcttcgaac ttaggattat ttcgtgcatc gagaaagata
    18301 gcgcctgtcg ctgcaaactc acctttggag tccttgttga gatacttttc attgtccttg
    18361 gcttggacta agggatagtc gatattggtg ccgtagacat tgatccaacc gaggacttcg
    18421 ggattgagtt cttggagttt gctgaagcct gaaaaactgg ccagctcatc ctgttgcgtc
    18481 gtgacaggtc gataagcttc gtactcactt gacgaagcta aggagtagac ttggttggaa
    18541 tcccaaaggg cgtagaaacc aaagagaaag atcaaagctg caaaaatgag cagcaagtga
    18601 ttgatcaccc catccacagc aagaacgaga cgcctagaca gactcttttt tggtttctca
    18661 cgcttggttt tcatcatctg cccccagccg tttaggcttc tctacgtttt ttagacaaga
    18721 caacaaatcc agccaaacca agaccaatca tcaaaatgaa tggaaggtta tcaatcaaga
    18781 gaccagtagg agtaacgtcg tcgattttgt tagtaacttt gttgtcgttt ttcttttcac
    18841 ctatgaggat atctgttaat gtaaaatcta catttttttg tccttgagat gtttgttctg
    18901 caccatttgt agtataaatt gatgaatctg tataacctgc tactccagtt tcagttactg
    18961 tatatttaga acctgctggt agttgttcaa ctgtaaatgt ttcaccatct ttaagagtaa
    19021 aattgtactc aactgtatct gctggggtca ctactgccaa cacctctgac ttagatccat
    19081 gttttactat aatattcgta acaggggttt ctgctgtttt atttgtactt ggaagttgaa
    19141 tcttgatatg gaattggaaa tctcttgttt tatcccctga agctccatca acttttttag
    19201 taattgttaa tgctttcgca ttggggtcta cttgactcgg atcatcagga ttaggctcct
    19261 tacttgcgtc tttggtatat gtgttatcaa ataaattata aacgccttgt tcacttggtt
    19321 ctactttagg ggcgttagat ttattatttt ccttaaagta tactgatgaa atatagacac
    19381 caccatttac tttattctta acaataacgt gcatttcata tgtacgtttg tcgtatctca
    19441 tggcatcaat tgttttacca tttttggtaa tcgcctgcca tccagttttt gcttccgcta
    19501 catcataaac gtattcacca gcatggccat aagcaactcc cgaaaaaatg tcttctgttg
    19561 ctttttgaat cttatcagca gttgggtgat catttttatt gtattctaca tttctatctg
    19621 gaattgcaac tccggtttga tatggcgcac cgttagaaga ttgtcctgtt ttttctgtaa
    19681 acttaaatgt aaatgtcgct gttggtgttg ttattccctc tgcaatattc aacgttttat
    19741 taatcttcac aacagcatta ttttcaccat ttgctttggt tattgctgaa ttatcatccg
    19801 caaaagtcgg gacacctctg aatacggaca aagcggttat agctgcaatg cttgcagtga
    19861 atagcttttt aaagaatgtt tttttcatga ttatttcttt tgttttttta ttttcttttt
    19921 tcattatgat catcaaaagc ctcgtcttct aattactgtt acaacttttc cttgagtatc
    19981 ctgtataggg atggttccaa aagcacgact gtctacagcc gttgttcgat tgtccccgag
    20041 gacaaaaagt tgtcctgctg ggactttcat cgagaaggtt gccccttcct tatagagcag
    20101 cgtttctttg tagatatctt gctcttgttg aggagaaccg ttgatgatga gaccattttc
    20161 gtttatatcg attgtacttc cttcggttgc tatgacacgc gccactcttt ccttaccttt
    20221 ataactatag actagcaaat caccaatcga atagcgttta tccaacctat aatagacgac
    20281 caagtcgcca tcttttaagg cgggcttcat gccatcgtca ttgtatctta ataatccaaa
    20341 gacgaaaaag taaagggtgg ctagtacaaa tccaaccatc agaagtttac tgactaagta
    20401 aaggatatct ccccagacac tcggcggaga cttatcccga ttgacttgtg ttactggttt
    20461 cttatgtttc tttttaagca gcatcacttc ctcctattca ccacaaggtg accaagtaag
    20521 atgataaaga agatccctcc tagaggaaga acgactccaa gcacgagagt gctactcaag
    20581 aagtcaattc ccgtttcagg tacgctgttt ttattattcg tgactgtcgc agacttattt
    20641 gcatcaagag ttccttcttt tttatcatag gacacctgat acccacgaga cgaacttgct
    20701 tcttcttcga tactataacg agcatttaga ggaaggtcga gaatcttgat ggttttatct
    20761 ttctttagat caactgtcgc cttaccattg gtgaattgta gcgttgtttt ttgattattt
    20821 actattgcac tatacgatcc attcaatggt ttattttgcg catcctttac gttaatggta
    20881 atcttaaagg attgcagaag attagcaaaa gcacctgtaa ctttttttgt gactgaaagt
    20941 gtcggaagtc tgtggttggt gacgattgtt tgaacatcct ctgacaaagt cccctcttgt
    21001 ttttcatagg tgacatgata tcccgttcta gagttctcta cttcttccac cttatagtgg
    21061 gtatcacgag gcagataact gagggcagtt tcctgactat ttttgagaga aatctgtgct
    21121 ttcccgttag tcactgttgt attcccaaac ttgccgttaa tcggcttgcc atccttatct
    21181 gaaagggtaa cattaattgt gaaagattta ttaggtgatt gttcaccttc taggattttc
    21241 tttacggtga gacttggaag ctgtcggtaa ataactttga aggatggttt ctctttcgta
    21301 cctgtctttt gaacttcgac tgtgtatccc ggagcgtctg gtgctttcag ttcgtagtca
    21361 tatcccttag ctacctttgc aaaactagta ttttcagtaa aactattcgt ttgaactgga
    21421 ctagtgacct ttcgatagga atcctttata cttccatctt tcttttggtt taagtttaat
    21481 tcgacactag gtggacgatt tgctgatgga tttgattttc catccacacc tttccactca
    21541 atctctacag gaaccgtcaa tggatctgac ggcttgaaag tgggcttctc agaatagtct
    21601 tttattttgg tacgtgaggt cccaatacca taggaatagg caacttttgc ccccttgttg
    21661 gagtagagtt tagtagcatc ggcttcttcg gcatcatcac tagtaagtgt cttatcaccc
    21721 gcaatcgcat caaatgcctc ttggctagag gtgacagtaa acttgagaac gtacttggcg
    21781 tcatcctcca aagtgtaact cggattaaac ttggctgtta cttccactaa tccctgttcg
    21841 ttcttcttag tttcaatcgt aacttgattg tcattcagtt cttgctcgtt accatccttg
    21901 attttgacga cacgaaggtg tgaagcatca ccaggtaaca gctgcacgta cttggacaag
    21961 acatctgata tagttacatg gtgtacgcct agaggtagaa ttttatctgt gatttctttg
    22021 aatttttgtt gcaagtcatt ttcattattg gcagaaaata tttcgttggg gattccagaa
    22081 ttataaccac tgatatatcc tttaagagat gtgatactat cgtttgaacc tatatatctg
    22141 aatttgattg aataaaagcc atctattttt ggagctatta aactagatat attcagcatt
    22201 cctctctcta aaccagaggt aaaccaatat ggagtatcaa tcatacgtcc cacatttgga
    22261 ttagaataat agttatatat tgttctccca ctattagact cgtaatacat attagcttcg
    22321 ccatctgaca gcagaataac tacttttttt gccccagccc ttgcactttg caacaatctt
    22381 tgagcttcag ttaacccagc atttatattt gtcccagtgc caatgcccgc gtccatacca
    22441 taagaatgac tagagtcagc aattgttaac gaactaacaa tattttttga gctattagaa
    22501 tccgtgctcc aagatactaa agttttagca tcatcatatg aagagatatt tgaataccaa
    22561 ccagctcaat ttggccaagt tccatattga taactcagat aatattcatt ataataacgg
    22621 ccattatact gattatcaat ctttccggca aaaccaacta tagacagacg gttttgggaa
    22681 ttagaaagaa ttgtatcaat gagcccctga cgaccattca cacctttcag tgtatgtttt
    22741 agtgcttcaa tcctactaat gtatcgtcta ctagagtaag accacacatc tcgctcttcc
    22801 atactccctg aaaaatcggc aaccaagact acatccaagg gttctgactg ggtctccgtc
    22861 tgggtctccg ttcccagttt agatgtgata tccagtgaca actcatactg gtcttcttgc
    22921 cctgatatcg gagtaatcgt tttgtgcaga gttgtctgag gttcagttgt tgaatctgca
    22981 cgtaaagaat taattggtgc acacaaaaaa agaatagcga gaaagataac taaactactg
    23041 gtaaaatatt gaatcttctt aaaatcatat tgaacgttca tgctatctcc cttgcta
//
LOCUS       DAVHIT010000028        21738 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVHIT010000028 DAVHIT010000000
VERSION     DAVHIT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.028"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            203..2185
                     /locus_tag="VVN16_000946"
     CDS             203..2185
                     /locus_tag="VVN16_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5572361.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMIPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2486..7789
                     /locus_tag="VVN16_000947"
     CDS             2486..7789
                     /locus_tag="VVN16_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU5572362.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGSVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPKGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDTDNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDSLKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDINFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(7956..10115)
                     /locus_tag="VVN16_000948"
     CDS             complement(7956..10115)
                     /locus_tag="VVN16_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU5572363.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10112..10708)
                     /gene="recU"
                     /locus_tag="VVN16_000949"
     CDS             complement(10112..10708)
                     /gene="recU"
                     /locus_tag="VVN16_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU5572364.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10774..11301
                     /locus_tag="VVN16_000950"
     CDS             10774..11301
                     /locus_tag="VVN16_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU5572365.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11371..11700
                     /gene="gpsB"
                     /locus_tag="VVN16_000951"
     CDS             11371..11700
                     /gene="gpsB"
                     /locus_tag="VVN16_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU5572366.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11721..12101
                     /gene="rnpB"
                     /locus_tag="VVN16_000952"
     ncRNA           11721..12101
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VVN16_000952"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12186..13343
                     /locus_tag="VVN16_000953"
     CDS             12186..13343
                     /locus_tag="VVN16_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU5572367.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13356..14750
                     /gene="mapZ"
                     /locus_tag="VVN16_000954"
     CDS             13356..14750
                     /gene="mapZ"
                     /locus_tag="VVN16_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU5572368.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     PASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14826..16250
                     /gene="gndA"
                     /locus_tag="VVN16_000955"
     CDS             14826..16250
                     /gene="gndA"
                     /locus_tag="VVN16_000955"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU5572369.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16262..16951
                     /locus_tag="VVN16_000956"
     CDS             16262..16951
                     /locus_tag="VVN16_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572370.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17050..18072
                     /gene="cbpC"
                     /locus_tag="VVN16_000957"
     CDS             17050..18072
                     /gene="cbpC"
                     /locus_tag="VVN16_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU5572371.1"
                     /translation="MKLLKKMMPIALAVFFFGLLATSTVFADTMGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIHGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDAENGDMKIGWTKVNGKWYYLNSSGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            18091..19089
                     /gene="cbpJ"
                     /locus_tag="VVN16_000958"
     CDS             18091..19089
                     /gene="cbpJ"
                     /locus_tag="VVN16_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU5572372.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMVTGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(19207..20439)
                     /locus_tag="VVN16_000959"
     CDS             complement(19207..20439)
                     /locus_tag="VVN16_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5572373.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20598..21068)
                     /locus_tag="VVN16_000960"
     CDS             complement(20598..21068)
                     /locus_tag="VVN16_000960"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572374.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            complement(20968..21306)
                     /locus_tag="VVN16_000961"
     CDS             complement(20968..21306)
                     /locus_tag="VVN16_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEU5572375.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
BASE COUNT         6797 a         3865 c         4881 g         6195 t
ORIGIN      
        1 agtaaaactg actaccagtt attatttaga aatagtatta aaaattcctt gactatgtga
       61 tatagttgag ggatttttaa atgatattca tattttttgc aaagatgttg tttgaaaaat
      121 aattttcaaa aattctgaaa attctgttga catctttctg aaaagagtct ataatggaga
      181 gaaagtttta aaggagaaaa tgatgaaaag ttcaaaacta tttgcccttg cgggcgtgac
      241 attattggcg gcgactactt tagctgcatg ctctggatca ggttcaagca ctaaaggtga
      301 gaagacattc tcatacattt atgagacaga ccctgataac ctcaactatt tgacaactgc
      361 taaggctgcg acagcaaata ttaccagtaa cgtggttgat ggtttgctag aaaatgatcg
      421 ctacgggaac tttgtgccgt ctatggctga ggattggtct gtatccaagg atggattgac
      481 ttacacttat actatccgta aggatgcaaa atggtatact tctgaaggtg aagaatacgc
      541 ggcagtcaaa gctcaagact ttgtaacagg actaaaatat gctgctgata aaaaatcaga
      601 tgctctttac cttgttcaag aatcaatcaa agggttggat gcctatgtaa aaggggaaat
      661 caaagatttc tcacaagtag gaattaaggc tctggatgat cagacagttc agtacacttt
      721 gaacaaacct gaaagttttt ggaactcaaa aacaaccatg ggtgtgcttg cgccagttaa
      781 tgaagagttt ttgaactcaa aaggggatga ttttgccaaa gctacggatc caagtagtct
      841 cttgtataat ggaccttatt tgttgaaatc cattgtgacc aaatcttctg ttgaatttgc
      901 gaaaaatccg aactactggg ataaggacaa tgtgcatatt gacaaagtta aattgtcatt
      961 ctgggatggt caagatacca gcaaacctgc agaaaacttt aaagatggta gccttacagc
     1021 agctcgtctc tatccaacaa gtgcaagttt cgcagagctt gagaagagta tgaaggacaa
     1081 tattgtctat actcaacaag actctattac gtatctagtt ggtacaaata ttgaccgtca
     1141 gtcctataaa tacacatcta agaccagcga cgaacaaaag gcatcgacta aaaaggctct
     1201 cttaaacaag gatttccgtc aggctattgc ctttggtttt gatcgtacag cctatgcctc
     1261 tcagttgaat ggacaaactg gagcaagcaa aatcttacgt aatatctttg ttccaccaac
     1321 atttgttcaa gcagatggta aaaactttgg cgatatggtc aaagagaaat tggttactta
     1381 tggggatgaa tggaaggatg ttaatcttgc agattctcag gatggtcttt acaatccaga
     1441 aaaagccaag gctgaatttg ctaaagctaa atcagcctta caagcagaag gtgtgacatt
     1501 cccaattcat ttggatatgc cagttgacca aacagcaact acaaaagttc agcgcgtcca
     1561 atctatgaaa caatccttgg aagcaacttt aggagcggat aatgtagtca ttgatattca
     1621 acaactacaa aaagacgaag taaacaatat tacatatttt gctgaaaatg ctgctggcga
     1681 agactgggat ttatcagata atgtcggttg gggtccagac tttgccgatc catcaaccta
     1741 ccttgatatc atcaaaccat ctgtaggaga aagtactaaa acatatttag ggtttgactc
     1801 aggggaagat aatgtagctg ctaaaaaagt aggtctatat gactacgaaa aactggttac
     1861 tgaggctggt gatgagacta cagatgttgc taaacgctat gataaatacg ctgcagccca
     1921 agcttggttg acagatagtg ctttgattat tccaactaca tctcgtacag ggcgcccaat
     1981 cttgtctaag atgataccat ttacaatacc atttgcattg tcaggaaata aaggtacaag
     2041 tgaaccagtc ttgtataaat acttggaact tcaagacaag gcagtcactg tagatgaata
     2101 ccaaaaagct caggaaaaat ggatgaaaga aaaagaagag tctaataaaa aggctcaaga
     2161 agatctcgca aaacatgtga aataactgtt gcaaaatata agaaaggatt tagtatttct
     2221 cttgaatgct gaatcctttt ttacatttgt aaagaaagat tctaaatgta ctgaccccca
     2281 aaagttggag cctctttttg tcagaataga gaaaattttt gttaatttta cttgtttcct
     2341 attgctttct cagctattat ttgttatatt aaaagtataa ttatttttta tttatcaggg
     2401 ttaagcattg cactttcaga ggaaggagta ttttttaaaa aggaaatgta aacgcttgct
     2461 aaaaaatgaa aggatttaga agtttatgaa taaaggatta tttgaaaaac gttgtaaata
     2521 tagtattcgg aaattttcat taggtgttgc ttctgttatg attggagctg cattctttgg
     2581 gacaagtccg gttcttgcag atagcgtgca gtctggttcc acggcgaact taccagctga
     2641 tttagctact gctcttgcaa cagcaaaaga gaatgatgga cgtgattttg aagcgcctaa
     2701 ggtgggagaa gaccaaggtt ctccagaagt tacagatgga cctaagacag aagaagaact
     2761 attagcactt gaaaaagaaa aaccggctga agaaaaacca aaagaggata aacctgcagc
     2821 tgctaaacct gaaacaccta agacggtaac ccctgaatgg caaacggtag agaaaaaaga
     2881 acaaaaggga acagtcacta tccgagaaga aaaaggtgtc cgctacaacc aactatcctc
     2941 aactgctcaa aatgataacg caggtaaacc agccctgttt gaaaagaagg gcttgaccgt
     3001 tgatgccaat ggaaatgcaa ctgttgattt aaccttcaaa gatgattctg aaaagggcaa
     3061 atcacgcttt ggtgtcttcc tgaaatttaa agataccaat aataatgttt ttgtcggtta
     3121 tgacaaggat ggctggttct gggagtataa atctccaaca actagcactt ggtatagagg
     3181 tagtcgtgtt gctgctcctg aaacaggatc aacaaaccgt ctctctatca ctctcaagtc
     3241 agacggtcag ctaaatgcca gcaataacga tgtcaatctc tttgacacag tgactctacc
     3301 agctgcggtc aatgaccatc ttaaaaatga gaagaagatc cttctcaagg cgggctctta
     3361 tggcaatgac cgtacggttg tcagcgttaa aacagacaat caagaaggcg taaaagcgga
     3421 tgatactcct gcccagaaag aaacaggttc ggttgttgac gatagcaagg tgacttatga
     3481 tacgatccag tctaaggtac ttaaggcagt aattgaccaa gccttccctc gtgtcaagga
     3541 atacaccttg aatggacata ctttgccagg acaggtgcaa cagttcaacc aagtctttat
     3601 caataaccac cgaatcaccc ctgaagtcac ttataagaaa atcaatgaga caacagcaga
     3661 gtacttgatg aagattcgcg atgatgctca cttaatcaat gcggaaatga cagtacgctt
     3721 gcaagttgtg gacaatcaat tgcactttga tgtgaccaag attgtcaacc acaatcaagt
     3781 cactccaggt caaaagattg atgacgaaag aaaactactt tcttctatta gtttcctcgg
     3841 caatgcttta gtctctgttt ctagtgatca aactggtgct aagtttgatg gggcaaccat
     3901 gtcaaacaat acgcatgtca gcggagatga tcatatcgat gtaaccaatc caatgaagga
     3961 tttggctaag ggttacatgt atggatttgt ttctacagat aagcttgctg ctggtgtttg
     4021 gagtaactct caaaacagct atggtggtgg ttcgaatgac tggactcgtt tgacagctta
     4081 taaagaaaca gtcgggaatg ccaactatgt aggaatccac agctctgaat ggcaatggga
     4141 aaaagcttat aagggcattg ttttcccaga atacacgaag gaacttccaa gggctaaggt
     4201 tgttatcact gaagatgcca atgcagacaa gaaagtcgat tggcaggatg gtgccattgc
     4261 ttatcgtagc attatgaaca accctaaagg ttgggaaaaa gttaaggata tcacagctta
     4321 ccgtatcgcg atgaactttg gttctcaagc acaaaaccca ttccttatga ccttggatgg
     4381 tatcaagaaa atcaatctcc acacagatgg tcttgggcaa ggtgttctcc ttaaaggata
     4441 tggtagtgaa ggtcacgact ctggtcactt gaactatgct gatattggta agcgtatcgg
     4501 tggtgtcgaa gacttcaaga ccctaattga gaaggctaag aaatatggag ctcatctagg
     4561 tatccacgtt aacgcttcag aaacttatcc tgagtctaaa tacttcaatg aaaaaattct
     4621 ccgtaagaat ccagatggaa gctatagcta tggttggaac tggctagatc aaggtatcaa
     4681 cattgatgct gcctatgacc tagctcatgg tcgtttggca cgttgggaag atttgaagaa
     4741 aaaacttggt gacggtctcg actttatcta tgtggacgtt tggggtaatg gtcaatcagg
     4801 tgataacggt gcctgggcta cccacgttct tgctaaagaa attaacaaac aaggctggcg
     4861 ctttgcgatc gagtggggcc atggtggtga gtacgactct accttccatc actgggcagc
     4921 tgacttgacc tacggtggct acaccaataa aggtatcaac agtgccatca cccgctttat
     4981 ccgtaaccac caaaaagatg cttgggttgg ggactacaga agttatggtg gtgcagccaa
     5041 ctatccactg ctaggtggct acagcatgaa agactttgaa ggctggcaag gaagaagtga
     5101 ctacaatggc tacgtgacta acctctttgc tcatgacgtc atgactaagt acttccaaca
     5161 cttcactgta agtaaatggg aaaatggtac accggtgact atgaccgata acggtagcac
     5221 ctataaatgg actccagaaa tgcgagtgga attggtagat actgacaata ataaagtagt
     5281 tgtaactcgt aagtcaaatg atgtcaatag cccacaatat cgcgaacgta cagtaactct
     5341 caacggacgt gtcatccaag atggttcagc ttacttgact ccttggaact gggatgcaaa
     5401 tggtaagaaa ctttctactg agaaggaaaa gatgtactac ttcaatacgc aggccggtgc
     5461 aacaacttgg acccttccaa gcgattgggc aaagagcaag gtttaccttt acaagctaac
     5521 tgaccaaggt aagacagaag agcaagaact aactgtaaaa gatggtaaaa ttaccctaga
     5581 tcttctagca aatcaaccat acgttctcta tcgttcgaaa caaaccaatc ctgaaatgtc
     5641 atggagcgaa ggcatgcaca tctatgacca aggatttaat agtggaacct tgaaacattg
     5701 gaccatttca ggcgatgctt ctaaggcaga aattgtcaag tctcaagggg caaacgatat
     5761 gcttcgtatt caaggaaaca aagaaaaagt tagtctcact cagaaattaa ctggcttgaa
     5821 accaaatact aagtatgccg tttatgtcgg tgtcgataac cgtagtaatg ccaaggcaag
     5881 tatcactgtg aatactggtg aaaaagaagt gactacttat accaataagt ctctcgccct
     5941 caactatgtt aaggcctacg cccacaatac acgtcgtgac aatgctacag ttaacgatac
     6001 aagttacttc caaaacatgt acgccttctt tacaacagga tcggacgtct caaatgttac
     6061 tctgacattg agtcgtgaag ctggtgatca agcaacttac tttgatgaaa ttcgtacctt
     6121 tgaaaacaat tcaagcatgt atggagacaa gcatgataca ggtaaaggca ccttcaagca
     6181 agactttgaa aatgttgctc agggtatctt cccatttgta gtgggtggtg tcgaaggtgt
     6241 cgaagacaac cgtactcact tgtctgaaaa acacgatcca tatacacaac gtggttggaa
     6301 tggtaagaaa gtcgatgatg ttatcgaagg aaattggtca ctcaagacaa atggactagt
     6361 gagccgtcgt aacttggttt accaaactat tccgcaaaac ttccgttttg aagcaggtaa
     6421 gacctaccgt gtaacctttg aatacgaagc aggttcagac aatacctatg cttttgtagt
     6481 cggtaaggga gaattccagt caggtcgtcg tggtactcaa gcaagcaact tggaaatgca
     6541 tgaattgcca aatacttgga cagattctaa gaaagccaag aaggcaacct tcctcgtgac
     6601 aggtgcagaa acaggggata cttgggtagg tatctactca actggaaatg caagtaatac
     6661 tcgtggtgat tctggtggaa atgccaactt ccgtggttat aacgacttca tgatggataa
     6721 tcttcaaatc gaagaaatta ccctaacagg taagatgttg acagaaaatg ctctgaagaa
     6781 ctacttgcca acggttgcca tgactaacta caccaaagag tctatggatt ctttgaaaga
     6841 ggcggtcttt aatctcagtc aggccgatga tgatatcagt gtggaagaag cgcgtgcaga
     6901 gattgccaag attgaagcct tgaagaatgc tttggttcag aagaaaacgg ctttggtagc
     6961 agatgacttt gcaagtctta cagctcctgc tcaggctcaa gaaggtcttg caaatgcctt
     7021 tgatggaaac ttatctagtt tatggcatac atcatggggc ggaggagatg taggcaagcc
     7081 tgcaaccatg gtcttgaaag aagcaactga aatcactgga cttcgttatg ttccacgtgg
     7141 atcaggttca aatggtaact tgcgtgatgt gaaacttgtt gtgacagatg agtctggcaa
     7201 ggagcatacc tttactgcaa ctgattggcc agataacaat aagccaaaag acattaattt
     7261 tggtaagaca attaaggcta agaaaattgt ccttacaggt actaagactt acggagatgg
     7321 tggcgataaa taccaatctg cagcggaact tatctttact cgtccacagg tagcagaaac
     7381 acctcttgac ttgtcaggct atgaagcagc tttggctaag gctcagaaat taacagacaa
     7441 agacaatcaa gaggaagtag ctagcgttca ggcaagcatg aaatatgcga cggataacca
     7501 tctcttgacg gaaagaatgg tggaatactt tgcagattat ctcaaccaat taaaagattc
     7561 tgctacgaaa ccagatgctc caactgtaga gaaacctgag tttaaactta gctctttagc
     7621 ttccgagcaa ggtaagacgc cagattataa gcaagaaata gctagaccag aaacacctga
     7681 acaaatcttg ccagcaacag gtgagagtca atctgacaca gccctcttcc tagcaggtgt
     7741 tagcctagcc ctatctgctc tctttgtagt aaaaacgaag aaagactagt atttagtaaa
     7801 acctcttaac aagattacgg aagcagtctc tatcttttcc aatgaggttt atagtacaga
     7861 aaaagcctga gaagatgtct tctcaggctt ttgttaagca cataaataca atagtgctat
     7921 gacaaaatca cccagaaaaa tctgggtgat aaatgttatg gttgtgctgg ttgaggattc
     7981 tgattttgtt gatcaggggt tgtatttgat tgttgcgtat tattgttagg attggtagtc
     8041 gtactattat ttgtgcttgg agtggttgag ctagactgtg aagttgaact atctgatgat
     8101 gagcttgaac tttcagttga tgggggttgt tgtggagcag gtgagttcca cgtagaacga
     8161 gcaccatttt taaatacgaa ttctccattt ctgtagagcc cctctggtat attccaatcc
     8221 tctggattgc ttccttcaga caggtaggtc atcatagagc ggtaaacttt ggcagcgacc
     8281 gtaaggccat tgcctacaag tggtgtcaga cggttagaat agcctgtcca tacagccatt
     8341 gaatatttac gcgtatagcc agcaaatagt tcatcaggtg ctacaaattg agaggtcttg
     8401 atgtggtttt caatttcctc gtctgtatag ttagaggttc ctgttttacc agcctgaggg
     8461 agccaagcaa gataggcatt tcgtccagtt ccataagtca agactgtttt catcatgtcg
     8521 gtcatcatat aggctgtcgt ttctttcatg gcacgagttc cgacattaga gaactctttt
     8581 tcactcccat cactaaagac gactttatgg atatacattg gtttatagta agttccacca
     8641 tttgcaaagg cagcgtaagc agcagccatc ttttcactac ttgctccata ttttttgtct
     8701 gattcggttg tgttacttga aatggcattt gagtagtgaa tacttgggta gtcgattccg
     8761 agaccattta ggaaagtctt ggcgcggttg agtccgacct tgtttagagt ttccacggct
     8821 gggacgtttc gcgattgttg cagggcgtat tgcaaggtga tgttgccaaa gtagccccta
     8881 tcccagttat aaacaggagt atttgtccca gggtaattat agggctcatc gtgaacgata
     8941 gtagcagttg aatcgtagac accgtactcc aaggcaggag catagtctgt gatcggtttc
     9001 atagttgatc cccagtcgcg gtttgtttct actgcttggt taattccgaa ggaaacatta
     9061 cttgactgat ggcgtgctcc tagctgggca atgactttac cgttagaaac atcaacaatg
     9121 gtagaagcga cttgcaattc atcgtctgga taggcaacgt attcgtctgt attgtaaata
     9181 tcccacagat gtttttgagc ttcttggtct acatttgtgt agacatccat cccagttgtg
     9241 agcaggttat agcctgtttc ttcttcaact tgattgatga cttccttgag gtaattatcc
     9301 atgtaagcag ggtaattact tgctgatttg agactttgta gtccatcagt aattggtgta
     9361 ttgactgctt tctcatactg ttcagcagag atgtagcctt gatttttcat ttcagataag
     9421 accaagtttc ggcggtcttg ggctgcttct ggatgtgaat aggggtcata ttggtttggt
     9481 gcctgaggca ttccagccag caaggctaac tgaggtaaac ttaaattatt gaggtcttta
     9541 ccatagtagt tttgagctgc tgtctgcatt ccatagttcc cattagacat gtagacctta
     9601 tttatatagt aggtcaagat ttcttgcttg gttgcttttt gttctaactg aatcgctaac
     9661 caagcttcct gagccttacg agaaatagtc tggtcggaag tcgaagttga aaagtaagtc
     9721 aacttaatta actgttgggt gagagttgat ccaccttgga gggaattgct ttgcagattg
     9781 cgcaagaaag ctcccaggat acggatggta tcaatccccc tgtggtcgaa gaagcgatgg
     9841 tcttcgatag aaacgattgc cttaaccaaa tctgtgggaa tatcattagc ttgggcattg
     9901 acgcggcgtt cagaacccaa gtcagcaatg agttgatttt tattgtcgta gattttacta
     9961 gaagttgttg caactagttt actctcggat aggctaggag ccttgctaac gtagtagaaa
    10021 aaaactcctc cgcctaagac aatggctgcg ataaccaagc ttaagaagct aatgctcaga
    10081 tacttgatta ggcgcagaat cgttggtttg ttcatcttgt tttaccacct aataaatgtt
    10141 ctttgataac attgagataa ggaatttgag ggaaggcacc agccttgatt tcatatccat
    10201 attctcgaat atattcaagt ggcattgatt tttgtccctt atcttgatga tagaagcgaa
    10261 tcaaatcgaa tgccggcaat aagtaggttt cttgctgaga agaaaagtga agaaggacaa
    10321 agcagattcc ttgttgggca aggacttgtt ccatatgctg aatctgatgt ggatgaaaat
    10381 ttttcatcgg aatcgcacgt ttttgttttg tttccttgac ttcaaagtcg atgtaatatc
    10441 cattataaac gccagaatag tccgtcgttg aagcttgtcg aaaataggct tcaacaatct
    10501 tggcacgact tcgttgtgga tagtccactt gtacgatttg aataggagtt ggtttcttat
    10561 gtataacagc caagccctga gacaaatagt agtcgttggt agcattgatc atcttttcaa
    10621 aagacattcc tcgatttgcg aaatttttgg gttgagaaag agatgtttgt cttttttgtg
    10681 atgaaacttt atgtggatag ttgaccataa ttctccttat tggtacaata acatcacttt
    10741 attataccat aatttgcaca gaaagggtta aaaatggcta cagctttggt tttaggctat
    10801 tctgcttttg atttgggttt attttctgat aaggatcctc gtcttaaatt gattaaaaaa
    10861 gcgatacgta aagatttaga ggctatggca gcagatgggg tgtcttggct tgtgtttaca
    10921 gggagtttgg gttttgaata ttgggtttta gaggttgctc aggagatgaa aaccgaatac
    10981 ggtttccagt tggccaccat ttttgctttt gaaacccatg gggaaaattg gaatgaaggc
    11041 aatcagatga aactgagtcg ctttaagcag gtagactttg tcaaatatgc ttatcctcgc
    11101 tatgaacaca aggggcagtt aagagattat cagcagtttt tgctggaaaa cacgactagt
    11161 tcctatcttt tttatgatga agaaaatgaa acaaaactag cttattttta ccaaaagatg
    11221 aaaaatcaag aggactattt tataaagaga ttaacatttg atcaattaaa tgaacttgct
    11281 gaaaattttt ccgaaaattg aagctttgac cttgattttt gtttgccttt ttttatataa
    11341 taatactagc aagcgagaat ggagagagac atggcaagta ttattttttc agcgaaagat
    11401 atttttgaac aagagtttgg acgtgaagtc cgtggctata ataaagtaga agttgacgag
    11461 tttttagacg atgtcatcaa ggactatgaa acctatgctg ccttggtcaa gtcacttcgt
    11521 caggaaattg cggatttgaa ggaagaatta actcgtaaac cgaaaccttc accagttcaa
    11581 gcagaacccc ttgaagcggc aattacaagt tctatgacga attttgatat tttgaaacgc
    11641 ctgaatagat tggaaaaaga agtttttggt aaacaaattt tagataactc agatttttaa
    11701 gtagttattt gagatgtgca atttttggat aatcgcgtga ggagaattgc ttctcatgag
    11761 gaaagtccat gctagcacag gctgtgatgc ctgtagtgtt tgtgctaggc gaaaccataa
    11821 gcctagggac gagaaatcgt tacggcagtt gaaatggcta agtccttgga taagccagag
    11881 taggcttgaa agtgccacag tgacggagtc tttctggaaa cagagagagt ggaacgcggt
    11941 aaacccctca agctagcaac ccaaattttg gtcggggcat ggagtacgcg gaaacgaacg
    12001 tagtattctg actgctatca gctagagctg ttagtggtag acagatgatt atcgaaggaa
    12061 gtggtcctag tcacttctgg aacaaaacat ggcttataga aaattgcata taggttgggg
    12121 ctgagaaatt ttctcaacct cattttttaa agtggacata tagaaaggtc ttgcaagact
    12181 gtaacatgaa aaaagaattt aatttaattg caactgtggc agcagggctt gaggctgtcg
    12241 ttggtcgtga agtgcgagag ttgggctacg attgtcaggt tgaaaatgga cgtgttcgtt
    12301 ttcaaggaga cgtgagagct attatcgaaa ccaacctttg gcttcgggca gcagatcgta
    12361 tcaaaattat cgtaggaacg ttcccagcta agacttttga agagctattt cagggagttt
    12421 tcgctttaga ttgggaaaat tatttaccac ttggagctcg gttcccgatt tcaaaagcta
    12481 aatgtgttaa gtccaaactt cacaatgagc ccagtgttca ggctatttct aagaaagctg
    12541 ttgtcaagaa attgcagaaa cactatgctc gcccagaagg ggttcctttg atggagaatg
    12601 gtccagagtt taagattgag gtctctattc tcaaagatgt ggcaactgtc atgattgata
    12661 cgaccgggtc tagcctcttt aaacgtggtt atcgtaccga aaaaggtggc gctcctatca
    12721 aggaaaatat ggcagcagcc attttacaac tttctaactg gtatccagac aagcctttga
    12781 ttgatccgac ctgtggttcg gggactttct gtattgaggc agttatgatt gctagaaaga
    12841 tggcgccagg tcttcgtcgc tcttttgcat ttgaggaatg gaactggatc agcgatcgct
    12901 tgattcaaga agtgcgcaca gaagcggcta aaaaagtaga acgtaagctt gagttggata
    12961 tcatgggctg tgatattgat gctcgcatgg tggaaattgc taaggccaat gctcaggcag
    13021 ctggtgttgc aggagacatt acttttaagc agatgcgcgt gcaggattta cgttccgata
    13081 aaatcaatgg agtaatcatt tccaatccgc cttatggtga acgtttgtca gatgatgcag
    13141 gggtgaccaa gctctatgct gagatggggc aagtatttgc accgctgaaa acttggagca
    13201 aatttatcct gactagtgat gaagcttttg aaagcaagta tggtagccaa gcagataaga
    13261 agcgtaagtt atacaacgga accttgaaag tggatctata tcaatatttt ggtcagcgtg
    13321 tcaaacggca agaggtaaaa tagaaaggga tactcatgag taaaaaaaga cgaaatcgtc
    13381 aaaaaaaaga aggtcaagaa ccgcaatttg attttgatga agcaaaagag ctaacagttg
    13441 gtcaagctat tcgtaaaaat gaagaagtgg aagcaggagt cttgcctgag gattccattt
    13501 tggacaagta tgttaagcaa cacagagatg aaattgaggc ggataagttt gcgactcgtc
    13561 aatacaaaaa agaggagttc gttgaaactc agagtctgga tgatttaatt caagagatgc
    13621 gtgaggctgt agagaagtca gaagcttctt cggaggaagt tccatcttct gaagacatct
    13681 tactaccctt gcctctggac gatgaggagc aaggcttgga tcctctattg ctagatgatg
    13741 aaaatccaac agaaatgact gaagaagtgg aagaggagca aaacctttct cgtctggatc
    13801 aagaggactc agaaaagaaa agcaaaaaag gctttatttt gaccgttttg gcgcttgtat
    13861 cagtaattat ttgtgtcagt gcttattatg tctaccgtca agtggctcgt tcgactaagg
    13921 aaattgaaac ttctcaatca actacagcca atcaatcgga tgtggatgat tttaatacac
    13981 tttatgacgc cttttacaca gatagcaata aaacggcttt gaaaaatagc cagtttgata
    14041 aactgagtca actcaagacc ttacttgata agctggaagg tagtcgtgaa catacgcttg
    14101 ccaaatctaa atatgatagt ctagtaacgc aaatcaaggc cattcaagat gtcaatgcac
    14161 aatttgaaaa accagctatt gtggatggtg tgttggatac caatgccaaa gccaaatcgg
    14221 atgctaaatt tacagatatt aaaactggaa atacggagct tgataaagtg ctagataagg
    14281 ctatcagtct tggcaagagc cagcaaacaa gtacttctag ctcaagttca agtcaaacta
    14341 gcagctcaag ctctagtcca gcaagttcaa atacgactag tgagccaaaa ccaagtagtt
    14401 caaatgagac tagaagtagt cgcagtgaag tcaatatggg tctctcgagt gcaggggttg
    14461 ctgttcaaag aagtgccagt cgtgttgcct ataatcagtc tgctattgat gatagtaata
    14521 actctgcctg ggattttgcg gatggtgtct tggaacaaat tctagcgact tcacgttcac
    14581 gtggctatat cactggagac caatatatcc ttgaacgtgt caatatcgtt aacggcaatg
    14641 gttattacaa cctctataag ccagatggaa cctatctctt tacccttaac tgtaagacag
    14701 gatattttgt cggaaatggc gctggtcatg cggatgactt ggattactaa gcagtcgtta
    14761 caaaattctt tcttttcaaa agtaaaaatg ataaaataaa acaaatgaaa caagaggagt
    14821 gtcaaatgac aaaagctaac tttggtgttg taggtatggc cgtaatgggt cgtaaccttg
    14881 cccttaatat tgaatctcgt ggttacacag ttgctatcta caaccgtagt aaagaaaaaa
    14941 cggaagatgt gattgcttgc catcctgaaa agaactttgt accaagctat gacgttgaaa
    15001 gttttgtaaa ctcaatcgaa aaacctcgtc gtatcatgct gatggttcaa gctggacctg
    15061 gtacagatgc tactatccaa gcccttcttc cgcaccttga caagggtgat atcttgattg
    15121 acggtggaaa tactttctac aaagatacca tccgtcgtaa tgaagaattg gcaaactcag
    15181 gtatcaactt tattggtact ggagtttctg gtggtgaaaa aggtgccctt gaaggtcctt
    15241 ctatcatgcc tggtggacaa aaagaggcct acgaattggt tgcggatgtt cttgaagaaa
    15301 tctcagctaa agcaccagaa gatggcaaac catgtgtgac ttacatcggt cctgatggag
    15361 ctggtcacta tgtgaaaatg gttcacaatg gtattgagta cggtgatatg caattgatcg
    15421 cagaaagcta tgacttgatg caacacttgc taggtctttc tgcagaggat atggctgaaa
    15481 tctttactga gtggaacaag ggtgaattgg atagctactt gatcgaaatc acagctgata
    15541 tcttgagccg taaagacgat gaaggccaag atggaccaat cgtagactac atccttgatg
    15601 ctgcaggtaa caagggaact ggtaaatgga ctagccaatc atctcttgac cttggtgtac
    15661 cattgtcact gattactgag tcagtgtttg cacgctacat ttcaacttac aaagaagaac
    15721 gtgtacatgc tagcaaggtg cttccaaaac cagctgcctt caactttgaa ggagacaagg
    15781 ctgaattgat tgaaaagatc cgtcaagccc tttacttctc aaaaatcatt tcatacgcac
    15841 aaggatttgc tcaattgcgt gtagcctcta aagaaaacaa ctggaacttg ccatttgcag
    15901 atatcgcatc tatctggcgt gatggctgta tcatccgttc tcgtttcttg caaaagatta
    15961 cagatgctta caaccgcgat gcagatcttg ccaaccttct tttggacgag tacttcttgg
    16021 atgttactgc taagtatcaa caagcagtgc gtgatatcgt agctcttgcg gttcaagctg
    16081 gtgtgccagt gccaactttc tcagcagcta ttacttactt tgatagctac cgttcagctg
    16141 accttccggc taacttgatc caagcacaac gtgactactt tggtgctcac acttaccaac
    16201 gtaaagacaa agaaggaacc ttccactact cttggtatga cgaaaaataa gtaggtcagc
    16261 catggggaaa cggattttat tacttgagaa agaacgaaat ctagctcatt ttttaagttt
    16321 ggaactccag aaagagcagt atcgggttga tctggtagag gaggggcaaa aagccctttc
    16381 catggctctt cagacagact atgatttgat tttattgaac gttaatctgg gagatatgat
    16441 ggctcaggat tttgcagaaa aattgagtcg aactaaacct gcctcagtca tcatgatttt
    16501 agatcattgg gaagacttgc aagaagagct ggaagttgtt cagcgttttg cagtttcata
    16561 catctataag ccagtcttta tcgaaaatct ggtagcgcgt atttcggcga tcttccgagg
    16621 tcgggacttc attgatcaac actgcagtct gatgaaagtt ccaaggacct accgcaatct
    16681 taggatagat gttgaacatc acacggttta tcgtggtgaa gagatgattg ctctgacccg
    16741 ccgtgagtat gaccttttgg cgacacttat gggaagtaag aaagtattga ctcgtgagca
    16801 attgttggaa agtgtttgga agtatgaaag tgcgaccgag acaaatatcg tagatgtcta
    16861 tatccgctat ctacggagca agcttgatgt taaaggccaa aaaagctaca ttaaaaccgt
    16921 gcgtggtgtt ggttacacca tgcaagaata gaaaagcagt tgcagttgtg taactgcttt
    16981 tttgaggagt ttttatatat tgacatacaa tctagttttt actacaatcg ttttaggagg
    17041 atatataaaa tgaagctttt gaaaaaaatg atgccaatcg cattagcagt ctttttcttt
    17101 ggtttgctag cgacaagtac agtatttgca gataccatgg gtggccgatt tgttgataag
    17161 gataatagaa aatattatgt aaaagatgat cataaagcaa tctattggca taaaatagac
    17221 ggtaaaactt actattttgg tgatattgga gagatggttg tcggttggca atacttagaa
    17281 attcatggaa caggttatcg tgatgattta tttgataatc gtccagtctt cgaaattggc
    17341 cttcaggaga agtggtacta ttttggacaa gatggtgctt tgctagaaca aacagataaa
    17401 caagtactag aggcaaaaac gtctgaaaat acaggaaaag tatacggtga acaatatcct
    17461 ctatctgctg aaaagagaac ttattatttt gataataatt atgctgtaaa gacaggctgg
    17521 atttatgaag acggcaattg gtattattta aataagctag gaaattttgg cgatgattct
    17581 tacaatccac taccaattgg tgaagttgct aagggttgga ctcaagattt tcatgttact
    17641 attgacattg atagaagcaa acctgctcca tggtactacc tagatgcttc aggtaagatg
    17701 cttacagatt ggcaaaaagt aaacggaaaa tggtattatt ttggctcctc tggttctatg
    17761 gcaacaggtt ggaaatatgt acgaggcaaa tggtattact tagataataa aaatggtgat
    17821 atgaaaacag gatggcaata ccttggtaac aagtggtact acctccgttc atcaggagct
    17881 atggtaactg gctggtatca agatggttca acttggtact atttagatgc tgaaaatggt
    17941 gatatgaaaa taggttggac aaaagtaaat ggaaaatggt attatctcaa ttcctctgga
    18001 gcaatggtta caggtagtca aactatcgat ggtaaagttt ataacttcgc ttcatctggt
    18061 gagtggattt aatgttggag gatatataaa atgaagcttt tgaaaaaaac tatgcaagct
    18121 ggactaacag taattttctt tggtttgcta gctacgaata cagtatttgc agataactct
    18181 gaaggatggc agtttgtcca agaaaacggt agaacctatt acaaaaaggg ggacctcaaa
    18241 gaaacctact ggcgagtgat tgatggtaag tactattatt ttgattctct atctggagag
    18301 atggttgtcg gctggcaata tatcccgttt ccatctaaag gtagtacaat tggtccttac
    18361 ccaaatggta tcagattaga aggttttcca aagtcagagt ggtactactt cgataaaaat
    18421 ggagtgctac aagagtttgt tggttggaaa acattagaga ttaaaactaa agacagtgtt
    18481 ggaagaaagt acggggaaaa acgtgaagat tcagaagata aagaagagaa acgttattat
    18541 acgaactatt actttaatca aaatcattct ttagagacag gttggcttta tgatcagtct
    18601 aactggtatt atctagctaa gacggaaatt aatggagaaa actaccttgg tggtgaaaga
    18661 cgtgcggggt ggataaacga tgattcgact tggtactatt tagatccaac aactggtatt
    18721 atgcaaacag gttggcaata tctaggtaat aagtggtact acctccgttc ctcaggagca
    18781 atggccactg gctggtatca ggaaggtacc acttggtatt atttagacca gccaaatggc
    18841 gatatgaaaa caggatggca aaaccttggg aacaaatggt actatctccg ttcgtcaggc
    18901 gccatggtga ctggttggta tcaagatggt tcaacttggt actacctaaa tgcaggtaat
    18961 ggagacatga agacaggttg gttccaggtc aatggcagat ggtactacgc ttatagctca
    19021 ggtgccttgg cagtgaatac gaccgtagat ggctattctg tcaactataa tggcgaatgg
    19081 gttcggtaat gacagggcgc ttataattat attagaacac tcgcttaatc aaagaagtga
    19141 ctttgttgta cttgacctga cgattgggga gattaaaagc atgtgaaatc tacacatgct
    19201 ttttcgttag ttatgagtgt tgaacttgac taaccattga caaaagataa gagcaattaa
    19261 aataagatac aaaaatatca atgctaactg caccgaaacg cccgatgcta tggatagggc
    19321 taatagggag agcagggacg gggcgagaat gtccactgtg ctcagagcag agcccaccgt
    19381 ccccatcttg tcactgggga tatgggcaaa gacagctgct tgtaggcgag ggctgagaat
    19441 gccgataatc gtagaactga tgaaactggt catgaaaatt acgatatagg cttgacaaag
    19501 gatactagtt attagagtca atagggaaat ctcacagaaa accaagaggt gcgtgaaacg
    19561 gatattttta aatagattac tgctggtcat attgccaagg atagctccac aagacatgat
    19621 gatggagagc agggcgagga gttgcccaat gctcagttgt gctagtgtct gttggatgaa
    19681 gcggagggca atcagccgag gggacacatc caagatggca atcatactgg tcgtcagaaa
    19741 cagtagtatg acggtatctt ttaacctcag gagcacagcc agcgactgtt taagatgttg
    19801 aaaatatttc ttaaaactca gtgctgtatg tgacatttca attctttttt caacctcata
    19861 caaggtatgt cgaataacat acaggcccaa aaaggcaatg acaaaagtca gagtgttgat
    19921 aagggaaata gtttgaatac ttataacatt gataaggaat gcgccagcca gattggcgac
    19981 aatacggaca accctcatca gagactgcct gaaccccatg gcatcatgca ggtcgtcctt
    20041 aattacactg atgtagaggg cgttcatcat gtaggcagac aggtagctca aggtgtcaga
    20101 gatgaggttg atgatgacaa tcataatgac tgaaactaat gaaaagggcg acaagacgag
    20161 aaatactgtc aaaatagcgt agagcaggat tttgattttg gcaatccaga gtgcgtgttt
    20221 gactctattt tcttgaaaat ctcctagaat cccgagaaag agttgcgata gtaggggaac
    20281 catctctgaa atggaaatca gtaaaatcgc tagcggagcg aaattgtact gagccacata
    20341 attgataatg gcgaggtaga aaatactgtc gccgaatgat gaaaaacagc tgtttagcag
    20401 caactgtcta tatatgttgt ttgtccaaaa taatttcata catttctcct tatgtctgca
    20461 catcgaatgg gaatttatta tttaatagac accaatatac cccctgcttc ctattccgaa
    20521 gtcaaacaaa tctgctttac cactatctcc ttgaaacatc agtaaaccgt gatcatctcg
    20581 ttcagaacat gctaaaatta cctgttggat tttttcatca gtaatttgtt ttgattgtag
    20641 acagtggttt gtagtaagct agttattccg tggcaatatc ctgtcatcat ttttagatga
    20701 tggttaaaaa caaattcttg atatttacta ataatatctt tgtttccgtc acaatcgtac
    20761 atacaaagat ataagattat tccggaaatt ccttcgcacc aagaaagttg taaatttcct
    20821 aaattttcag tattagaagt aactttttct aataattttt ttaattcagc atggaatgta
    20881 tggagatcat tatagaacat agaaggttct aagactttag aataggcaaa taaagcatat
    20941 gcaattccac aatatccatg agcaaagcta tagtctaaaa tgctttcttt ggctgtatct
    21001 ctttgtagga aataatgata aatttcccca tatactattg tggatgaaag ttttaagagt
    21061 attgtcattg gtaaattgat agtattttat taatgataat aggacaccag attttcccaa
    21121 tgcaaagtct atttcctcta gagtatcata attctctatt aatttcttag cggtgacgtt
    21181 tgctaattgt agatagtatt gatttttact gatagaaaat aaatgtagga gtaaccatag
    21241 atagcctgca tcaccaaata gcagtgagta ttgatattca tatgtatgtg tcgttctaat
    21301 tgtcactgcc ttcttgatga tagagagtat tttagaatcc agttctgggg ggtacaattt
    21361 atttagagga aacagtagtc cagctattcc gtgttggaat gataaattac ttacgaaatt
    21421 tctaaattct gttgaagaag tgatccttcc ttcagaattt acagttttat ctagtaaaaa
    21481 ttgtagaaca ctattacatt ctgttttgta atcatattta tgttcctcta tttttttaat
    21541 tttgactaaa ttatttgttt gacttaaatt tagtagacat tgtaaccaaa atagttgctc
    21601 atctgtgagc tgaccttgag tatgtgatag ttctagtaat tgaataggat cctttaggta
    21661 gattcaatca atctataaca caacaacata ataacacttt taggataact tatcaaatat
    21721 tatagtaaaa tgaaataa
//
LOCUS       DAVHIT010000029        21658 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVHIT010000029 DAVHIT010000000
VERSION     DAVHIT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.029"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            138..2351
                     /locus_tag="VVN16_000962"
     CDS             138..2351
                     /locus_tag="VVN16_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU5572376.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYSRVAKRLGLNVELEEPAIFDFFNPSFRESYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPMYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLAFKSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTI
                     PVFAKNGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMIEDQNGQRYKTRLSIDW
                     EMGMVELTLQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTARFEWKDNKTTSLNDE
                     VFRLLKPASLPYELKDRLLNQFINVKNSHELMNILHHQDKELRGRLLEMIFTNEN"
     gene            complement(2573..3049)
                     /locus_tag="VVN16_000963"
     CDS             complement(2573..3049)
                     /locus_tag="VVN16_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU5572377.1"
                     /translation="MTTLYDFSVLNQDNQEISLDAYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFKILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQKLL"
     gene            complement(3046..3624)
                     /locus_tag="VVN16_000964"
     CDS             complement(3046..3624)
                     /locus_tag="VVN16_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU5572378.1"
                     /translation="MKKEDFTTRLLRNLFHIQGPFDECRQEVIYKACARSMIQIFYSS
                     FILFLFYILFGSFIEIVRNVMPYIYFGLILFMSIKAQKAVQELHLEKDDKSEIILKTY
                     SKAQIKFRSWVVFIGIQIGLFTLLIFHKVFVQQMSFSDFGKLLIQFDKSGPFLMYGLI
                     IGSIFGTLTYGFLSLQEEKTPKNTKQKEKSKQ"
     gene            complement(3637..3978)
                     /locus_tag="VVN16_000965"
     CDS             complement(3637..3978)
                     /locus_tag="VVN16_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572379.1"
                     /translation="MKSLVTLLTYHLLFSSLLIFIIISGNLPIREIFLLLVFIPALNK
                     GLTYLKIDSPKTRILNLALCFMILSLPQLMLVSGNWKYYLLLTIASISSITYLYYLYQ
                     LVKEVNQKPLI"
     gene            complement(3989..4531)
                     /locus_tag="VVN16_000966"
     CDS             complement(3989..4531)
                     /locus_tag="VVN16_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU5572380.1"
                     /translation="MKKETFTEKLIKRTYGISDPLDEYKRREADRIGNQVFIFLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAASYVTYQMKKTGITTIEPDMLSKKE
                     SKQLRYPGLKAGLFFGLWIFFITPLLDILIGEGQDYFQSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKAKKDQDED"
     gene            complement(4547..4741)
                     /locus_tag="VVN16_000967"
     CDS             complement(4547..4741)
                     /locus_tag="VVN16_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5572381.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            4907..5857
                     /gene="rsmH"
                     /locus_tag="VVN16_000968"
     CDS             4907..5857
                     /gene="rsmH"
                     /locus_tag="VVN16_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5572382.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAINNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            5869..6186
                     /gene="ftsL"
                     /locus_tag="VVN16_000969"
     CDS             5869..6186
                     /gene="ftsL"
                     /locus_tag="VVN16_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU5572383.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            6190..8442
                     /gene="pbpX"
                     /locus_tag="VVN16_000970"
     CDS             6190..8442
                     /gene="pbpX"
                     /locus_tag="VVN16_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU5572384.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSIFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            8444..9424
                     /locus_tag="VVN16_000971"
     CDS             8444..9424
                     /locus_tag="VVN16_000971"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5572385.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            10033..12138
                     /locus_tag="VVN16_000972"
     CDS             10033..12138
                     /locus_tag="VVN16_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5572386.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQH
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            12590..12925
                     /locus_tag="VVN16_000973"
                     /pseudo
     CDS             12590..12925
                     /locus_tag="VVN16_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(12992..13474)
                     /locus_tag="VVN16_000974"
     CDS             complement(12992..13474)
                     /locus_tag="VVN16_000974"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU5572387.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(13569..15053)
                     /locus_tag="VVN16_000975"
     CDS             complement(13569..15053)
                     /locus_tag="VVN16_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU5572388.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            15206..16813
                     /locus_tag="VVN16_000976"
     CDS             15206..16813
                     /locus_tag="VVN16_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU5572389.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            16972..17145
                     /locus_tag="VVN16_000977"
     CDS             16972..17145
                     /locus_tag="VVN16_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU5572390.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNTVN"
     gene            complement(17138..17663)
                     /locus_tag="VVN16_000978"
                     /pseudo
     CDS             complement(17138..17663)
                     /locus_tag="VVN16_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            17885..19330
                     /gene="cps4A"
                     /locus_tag="VVN16_000979"
     CDS             17885..19330
                     /gene="cps4A"
                     /locus_tag="VVN16_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU5572391.1"
                     /translation="MSRRFKKSRSQRVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLATYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAVIQDVMEGR"
     gene            19332..20063
                     /locus_tag="VVN16_000980"
     CDS             19332..20063
                     /locus_tag="VVN16_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEU5572392.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKVKELFVDNPRKIIMDQLI"
     gene            20072..20764
                     /gene="cpsC"
                     /locus_tag="VVN16_000981"
     CDS             20072..20764
                     /gene="cpsC"
                     /locus_tag="VVN16_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU5572393.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSMTRIYVVNRNQGEKSGLTNQDLQAGSSLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKDLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRSTLIGFLAGVIGTSVIVLILELLDTRVKRPKDIEDTLHMT
                     LLGIVPNLNKLK"
     gene            20774..21463
                     /gene="cpsD"
                     /locus_tag="VVN16_000982"
     CDS             20774..21463
                     /gene="cpsD"
                     /locus_tag="VVN16_000982"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEU5572394.1"
                     /translation="MPTLEIAQKKLEFVKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PEEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGTVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GFYGNYGKKII"
BASE COUNT         6620 a         3764 c         4825 g         6449 t
ORIGIN      
        1 ttttgtccac tacatttttt caattgacta ttatttgtca tagatataac agatagaaca
       61 ttgcattcag aataatgaaa aacgcatcaa aaatggtgcg ttctgttttt atgctaccat
      121 aaaaggagga aaacaaattg aaaattttta agggagagtt ttatcgaatc tctgtattaa
      181 cagacaagct agtaaggtta gaatactctc aaactggaag ttttgaggat agaacgacac
      241 aacttatcta taatagagat tttggccaag tttcgttaga ttatatcgag acatcaaacg
      301 tactagatat tatgacggac tattttcatc tgcactttaa taaaggagaa tttaacgccg
      361 aaaatttatt tatagaatta aaaggaaatt ttgccgtata tggtagtcgc tggtattttg
      421 gtgaatctat tgaaacgtta aaaggaacag ctcggactct ggataaggca gatggagcaa
      481 tctcgttaga agatggaatt attagccgaa atggtatagc cttattggat gattctcaag
      541 gatttatttg ggatgaacaa tctggttata ttgagagaga aaatcaaatt gacctgtatt
      601 tctttgccta tgggcatgat tatagaggag caatcagaga tttttaccat ttgactggtt
      661 caacaccctt gttgccaaga tatgctttag gcaattggtg gagtagatat tggccttata
      721 cgtcggatga atacttggat ttaatagaca gatttgaaac agagaaaatt ccattatcta
      781 tcggtgtgtt agatatggat tggcatataa ctgacattcc agctcgcttt ggaagtggct
      841 ggacaggata tagttggaat agaaacttaa taccaaatcc agaacagtta ttgcaacaac
      901 ttcatgatag aaagctaaaa ctctccttaa atgtccatcc tgctgatggt atacgggctt
      961 atgaagaagc ttattctcga gtcgccaaac gattagggtt aaatgtagaa ttagaagaac
     1021 ctgctatttt tgattttttt aatccctctt ttagggaatc ctactttaaa gatgttcatt
     1081 atgagctaga aaagcaggga gtagattttt ggtggattga ctggcaacaa gggactcaag
     1141 gtatgttgga tccactgtgg cttttaaacc attatcacta tcaggatagt tgtaaaaatg
     1201 cagaaggtgg cttaatttta tcaaggtatg caggtcctgg tagtcaccgc taccctgttg
     1261 gtttttcagg agataccatt attagttgga attccttaag atttcaacct tattttacag
     1321 cgacagcatc aaatatcggt tatagttggt ggagccatga tatcggtggg cacatgctgg
     1381 gggattatga cgaagagcta caaactagat ggctacagtt tggggttttt agtccgatta
     1441 ctcgcttaca tagttctaga agtcccttta atagtaaaga accttggttt ttttcagaaa
     1501 caacatctaa gattatgaag aaataccttc gtttgagaca tcagatgatt ccctatctat
     1561 acactatgaa tgtacagaca cacgaggaag gtgccccatt aatcagcccg atgtattatt
     1621 tctacccaga gaatgatgag agttataatg ttccaaatca atactttttt ggaacagaac
     1681 tgatggtggc tcccattgta gaaaagatgg acttggcatt caaatctgca aaagtggatg
     1741 tatggttccc tgaaggtgaa tggtatgact tcttttcaga gaaaaaatat acaggtggtg
     1801 tgaagttgag tgtttataga gacatttcga ctatacctgt gtttgcaaaa aatggtgcaa
     1861 tcattccctt ggttggttct gagatagata tgggtgttga tttacctgaa gttgtagatt
     1921 ggtatgtatt cccaggcaaa caacattctt ttgaaatgat tgaagatcaa aatggtcaaa
     1981 gatataaaac aagattatca atcgactggg aaatggggat ggtagagcta acattacaag
     2041 gagattctag tatcgttcca agcaatagaa aacatagaat tcattttaaa ggaacgaatg
     2101 tgtctataat tgaattgcca aataagaatg atacagctag atttgaatgg aaagataata
     2161 aaacgacatc tctaaatgat gaagttttta gactactaaa gccggcttct cttccatatg
     2221 aattaaaaga tagattgtta aatcaattta tcaatgtcaa aaattctcat gagttaatga
     2281 atatcttgca tcatcaggat aaggaattga gagggcgttt gttggaaatg atatttacta
     2341 atgaaaacta attgagataa ttagaaagtc atttgtttag tgtatatgaa tattctgtat
     2401 ggtattcgtt atcactggtc acattgaaaa tgtgaaataa gtagctacaa catagacttt
     2461 acaacacttc atgtttcata tcatggggtg tttttctatt taagtcaaag cccatcaaaa
     2521 aatcccacta ttcattaggc taaaggaaac ctaatcatag tgagattgtg aattatagta
     2581 gtttttgtat ggcctcttca atttgttttg ggtctgtttt tgaagagaag cgttcaaaga
     2641 cctgcccatc acgaccgatg agaaacttag cgaaattcca ttcgattcgt tttcctagtg
     2701 ggccggattt ctggtctttt aaccagacat agagagggtc tgcttcctta ccgttgacct
     2761 taatcttggc aaaacgtggg aaggtggttt gaaaatgtag gctacagaag gcgttgattt
     2821 cctctgcgct gccgggtgct tgtcccataa actgattgca agggaaatcc aatattttga
     2881 agccctgatc ttgatagcgt tcatagagtt cttgaagtcc ttggtactgg ggcgttaaac
     2941 cacatccagt agcagtgttg acaatcaaga gtaccttacc acgataggca tctagtgaga
     3001 tttcttgatt gtcttggttc aagactgaaa aatcgtatag tgtagtcatt gcttgctttt
     3061 ctccttctgt ttggtatttt taggagtttt ttcctcctgt agggatagaa agccgtaggt
     3121 cagggttcca aaaatgctac cgataatcag gccatacatt aggaaaggac cacttttatc
     3181 aaattgtatg agcaactttc caaaatctga aaaggacatc tgctgaacga agactttatg
     3241 aaaaatgagt agggtaaaga ggccaatctg aataccgata aacacaaccc agcttcgaaa
     3301 tttgatttgg gctttgctgt aggttttgag gatgatttct gacttgtcat ccttttcaag
     3361 atgaagctct tggacggctt tttgagcttt tatactcatg aacaaaatga gtccaaaata
     3421 gatgtagggc ataacatttc gaacaatttc tataaagctt ccgaacaaaa tatagaatag
     3481 gaagagaatg aaggaagagt aaaagatttg gatcatggaa cgggcgcaag ccttgtagat
     3541 aacctcttga cggcattcat caaaagggcc ttggatgtga aagagatttc gaagtaggcg
     3601 agtggtgaag tcttcttttt tcatgctgat aacctcctaa atgagcggtt tttgattgac
     3661 ttctttaacg agttgataga gataataaag ataagtgata gaagaaatgc tagcgatagt
     3721 cagcagtaaa taatatttcc aattgcctga gacgagcatc agttgtggca aagatagaat
     3781 cataaagcat agtgctaagt tgagtatgcg cgttttgggg gagtcaatct ttagataagt
     3841 cagtcctttg ttgagggctg gaataaagac tagaaggagg aatatctctc tgattggtaa
     3901 gttccctgaa atgatgatga agatgagcag agaactgaac aaaagatgat aggtaagtaa
     3961 agttactagt gatttcataa ggcacctcct aatcctcatc ttgatctttc ttggcttttg
     4021 caatgcgacg ggagatgagg aactgtatgc ttgccccgaa gaagatagaa ccgagaatgc
     4081 ttgatacacc atttcttata gtgagaagag actgaaaata gtcctgacct tcacctatga
     4141 gtatatcgag aagaggagtt ataaaaaata tccatagacc aaagaacaaa cctgctttca
     4201 gacctgggta gcgaagttgc ttactttctt tcttactcag catatctggt tcaatagttg
     4261 taatccctgt ttttttcatt tggtaggtga catagctagc agcgatgagg gcaatcacta
     4321 aaatcagagg aggatagatt agagccactt cttgaggata tttataggcc agaaggagtg
     4381 gaataagatt tccgaaaatc atcagataaa agaggaagat aaagacttga ttgccgatac
     4441 gatcagcctc gcgtcgtttg tattcgtcaa gtggatcaga aataccgtat gtgcgtttga
     4501 tgagtttttc agtaaaggtt tcttttttca tgagttggct ccttttttaa aaatcttcct
     4561 cccaaaagag actgttgagg tcagtttgga ggctacgggc gagattgaga cagagttcca
     4621 gggttggatt gtacttgtcg ttttcaatca tattgatggt ctgtctcgag acaccgatat
     4681 ccttggcgag ttcgagctgg gaaataccca attccttgcg aaattctttc acacgattca
     4741 tctggtctcc tttctgattt gtgtcgtata tatttgacta tattatagtc ttttaaatat
     4801 gaagtgtcaa gtatttttga catatttttt gaagaaatag tagtctcctt gtccgatttg
     4861 tctgactagt gcaagctgtt cggatttgtg gtaaaataga taagatatga caaaagaatt
     4921 tcatcatgta acggtcttac tccacgaaac gattgatatg ctcgacgtaa agcctgacgg
     4981 tatctacgtt gatgcgactt taggtggagc gggccatagc gaatatttat taagtaaatt
     5041 aagtgaaaaa ggtcatctct atgcctttga ccaggaccag aatgccatta acaatgcgca
     5101 aaaacgcttg gctccctata ttgagaaggg gatggtgacc tttatcaagg ataacttccg
     5161 tcatttacag gcacgtttgc gcgaagctgg tgttcaggaa attgatggaa tttgttatga
     5221 cttgggagtg tctagtcctc aattggacca gcgtgagcgt ggtttttctt ataaaaagga
     5281 tgcgccactg gacatgcgga tgaatcagga tgctagtctg acagcctatg aagtggttaa
     5341 tcattatgac tatcatgact tggttcgtat tttcttcaaa tacggtgagg ataaattctc
     5401 taaacagatt gcgcgtaaga ttgagcaagc gcgtgaagtg aagccgattg agacaacgac
     5461 tgagttagca gagattatca agttggtcaa acctgccaag gaactcaaga agaagggtca
     5521 tcctgctaag cagattttcc aggctattcg aattgaagtc aatgatgaac tgggggcggc
     5581 agatgagtcc atccagcagg ctatggatat gttggctctg gatggtagaa tttcagtgat
     5641 tacctttcat tccttagaag accgcttgac caagcaattg ttcaaggaag cttcaacagt
     5701 tgaagttcca aaaggcttgc ctttcatccc agatgatctc aagcccaaga tggaattggt
     5761 gtcccgtaag ccaatcttgc caagtgcgga agagttagaa gccaataacc gctcgcactc
     5821 agccaagttg cgcgtggcca gaaaaattca caagtaagag ggaaaaagat ggcagaaaaa
     5881 atggaaaaaa caggtcaaat actacagatg caacttaaac ggttttcgcg tgtggaaaaa
     5941 gctttttact tttccattgc tgtaaccact cttattgtag ccattagtat tatttttatg
     6001 cagaccaagc tcttgcaagt gcagaatgat ttgacaaaaa tcaatgcgca gatagaggaa
     6061 aagaagaccg aattggacga tgccaagcaa gaggtcaatg aactattacg tgcagaacgt
     6121 ttgaaagaaa ttgccaattc acacgatttg caattaaaca atgaaaatat tagaatagcg
     6181 gagtaagata tgaagtggac aaaaagagta atccgttatg cgaccaaaaa tcggaaatcg
     6241 ccggctgaaa acagacgcag agttggaaaa agtctgagtt tattatctat ctttgttttt
     6301 gccatttttt tagtcaattt tgcggtcatt attgggacag gcactcgctt tggaacagat
     6361 ttagcgaagg aagctaagaa ggttcatcaa accacccgta cagttcctgc caaacgtggg
     6421 actatttatg accgaaatgg agtcccgatt gctgaggatg caacctccta taatgtctat
     6481 gcggtcattg atgagaacta taagtcagca acgggtaaga ttctttacgt agaaaaaaca
     6541 caatttaaca aggttgcaga ggtctttcat aagtatctgg acatggaaga atcctatgta
     6601 agagagcaac tctcgcaacc taatctcaag caagtttcct ttggagcaaa gggaaatggg
     6661 attacctatg ccaatatgat gtctatcaaa aaagaattgg aagctgcaga ggtcaagggg
     6721 attgatttta caaccagtcc caatcgtagt tacccaaacg gacaatttgc ttctagtttt
     6781 atcggtctag ctcagctcca tgaaaatgaa gatggaagca agagcttgct gggaacctct
     6841 ggaatggaga gttccttgaa cagtattctt gcagggacag acggcattat tacctatgaa
     6901 aaggatcgtc tgggtaatat tgtacccgga acagaacaag tttcccaacg aacgatggac
     6961 ggtaaggatg tttatacaac catttccagc cccctccagt cctttatgga aacccagatg
     7021 gatgcttttc aagagaaggt aaaaggaaag tacatgacag cgactttggt cagtgctaaa
     7081 acaggggaaa ttctggcaac aacgcaacga ccgacctttg atgcagatac aaaagaaggc
     7141 attacagagg actttgtttg gcgtgatatc ctttaccaaa gtaactatga gccaggttcc
     7201 actatgaaag tgatgatgtt ggctgctgct attgataata atacctttcc aggaggagaa
     7261 gtctttaata gtagtgagtt aaaaattgca gatgccacga ttcgagattg ggacgttaat
     7321 gaaggattga ctggtggcag aatgatgact ttttctcaag gttttgcaca ctcaagtaac
     7381 gttgggatga ctctccttga gcaaaagatg ggagatgcta cctggcttga ttatcttaat
     7441 cgttttaaat ttggagttcc gacccgtttc ggtttgacgg atgagtatgc tggtcagctt
     7501 cctgcggata atattgtcaa cattgcgcaa agctcatttg gacaagggat ttcagtgacc
     7561 cagacgcaaa tgattcgtgc ctttacagct attgctaatg acggtgtcat gctggagcct
     7621 aaatttatta gtgccattta tgatccaaat gatcaaactg ctcggaaatc tcaaaaagaa
     7681 attgtgggaa atcctgtttc taaagatgca gctagtctaa ctcggactaa catggttttg
     7741 gtagggacgg atccggttta tggaaccatg tataaccaca gcacaggcaa gccaactgta
     7801 actgttcctg ggcaaaatgt agccctcaag tctggtacgg ctcagattgc tgacgagaaa
     7861 aatggtggtt atctagtcgg gttaaccgac tatattttct cggctgtatc gatgagtccg
     7921 gctgaaaatc ctgattttat cttgtatgtg acggtccaac aacctgaaca ttattcaggt
     7981 attcagttgg gagaatttgc caatcctatc ttggagcggg cttcagctat gaaagactct
     8041 ctcaatcttc aaacaacagc taaggcttta gagcaagtaa gtcaacaaag tccttatcct
     8101 atgcctagtg tcaaggatat ttcacctggt gatttagcag aagaattgcg tcgcaatctt
     8161 gtacaaccca tcgttgtggg aacaggaacg aagattaaaa acagttctgc tgaagaaggg
     8221 aagaatcttg ccccgaacca gcaagtcctt atcttatctg ataaagcaga ggaggttcca
     8281 gatatgtatg gttggacaaa ggagactgct gagacccttg ctaagtggct caatatagaa
     8341 cttgaatttc aaggttcggg ctctactgtg cagaagcaag atgttcgtgc taacacagct
     8401 atcaaggaca ttaaaaaaat tacattaact ttaggagact aatatgttta tttccatcag
     8461 tgctggaatt gtgacatttt tactaacttt agtagaaatt ccggccttta tccaatttta
     8521 tagaaaggcg caaattacag gccagcagat gcatgaggat gtcaaacagc atcaggcaaa
     8581 agctgggact cctacaatgg gaggtttggt tttcttgatt acttctgttt tggttgcttt
     8641 ctttttcgcc ctatttagta gccaattcag caataatgtg ggaatgattt tgttcatctt
     8701 ggtcttgtat ggcttggtcg gatttttaga tgactttctc aaggtctttc gtaaaatcaa
     8761 tgaggggctt aatcctaagc aaaaattagc tcttcagctt ctaggtggag ttatcttcta
     8821 tcttttctat gagcgcggtg gcgatatcct gtctgtcttt ggttatccag ttcatttggg
     8881 atttttctat attttcttcg ctcttttctg gctagtcggt ttttcaaacg cagtaaactt
     8941 gacagacggt gttgacggtt tagctagtat ttccgttgtg attagtttgt ctgcctatgg
     9001 agttattgcc tatgtgcaag gtcagatgga tattcttcta gtgattcttg ccatgattgg
     9061 tggtttgctc ggtttcttca tctttaacca taagcctgcc aaggtcttta tgggtgatgt
     9121 gggaagtttg gccctaggtg ggatgctggc agctatctct atggctctcc accaagaatg
     9181 gactctcttg attatcggaa ttgtgtatgt ttttgaaaca acttctgtta tgatgcaagt
     9241 cagttatttc aaactgacag gtggtaaacg tattttccgt atgacgcctg tacatcacca
     9301 ttttgagctt gggggattgt ctggtaaagg aaatccttgg agcgagtgga aggttgactt
     9361 cttcttttgg ggagtgggac ttctagcaag tctcctgacc ctagcaattt tatatttgat
     9421 gtaagaatgg caccctgatg tttcagggtg tttttgtgtt taaatacaca atgaaaatca
     9481 aagaacaaac tagaaagcta actttaggct actcaaaata tagtagattg aaactaaaat
     9541 agtacacatc tacttctaaa atattgttag aaatcgattt gactgtcctg attgatttgt
     9601 cctgttctta tttcatttta ctatacagtt ttgagatggt tgatagaact gacgaagtca
     9661 gctcaaaaca cggttttgag gttgtagata aggagaagct gatgtggttt gaagaggttt
     9721 tcgaagagtg taaaaaaata ttggtcagtt aaagtcaaaa ctcttgacct tttctgacca
     9781 atgtagtata ttatgaacgt aaggtaaatg aaagccttac tagaacactt atttaaagta
     9841 aaatagaaag aggtgggtct atgtttcatc tagaaatctt cagaagtaaa gatagtctac
     9901 tcctgcttga aaaagaaaaa ccagaaatag tacgtagggt agcgatctag ctagtctaaa
     9961 ttttttaaaa caatggtcaa agatagtcaa tatcagtaat cataactaag taaacaaaaa
    10021 gaggtaaaga atatgaataa caactttaat aattttaata acatggatga tttatttaac
    10081 caattgatgg gtggtatgcg aggatacagt tctgaaaatc gccgttactt aattaatgga
    10141 cgcgaagtca cacctgagga atttgctcac tatcgtgcga ctggtcaatt accaggaaat
    10201 gcagaaactg atgtgcaaat gccacaacag gcatcaggta tgaaacaaga cggtgtcctt
    10261 gcaaaactag gtcgaaactt aacagcagaa gcgcgtgagg gcaagttgga tcctgttatc
    10321 ggacgaaaca aggaaattca agaaacatct gaaatcctct cacgccgcac caagaacaat
    10381 cctgttttgg tcggagatgc aggtgttggt aagacagcag ttgtcgaagg tctagcgcaa
    10441 gccattgtga acggagatgt tcctgctgct atcaagaaca aggaaattat ttctattgat
    10501 atctcaggtc ttgaggctgg tactcaatac cgtggtagct ttgaagaaaa tgtccaaaac
    10561 ttagtcaatg aagtgaaaga agcagggaat attatcctct tctttgatga aattcaccaa
    10621 attcttggtg ctggtagcac tggtggagac agtggttcta aaggacttgc ggatattctc
    10681 aagccagctc tctctcgtgg agaattgaca gtgattgggg caacaactca agacgaatac
    10741 cgtaacacca tcttgaagaa tgctgctctt gctcgtcgtt tcaacgaagt gaaggtcaat
    10801 gctccttcgg cagagaatac ttttaaaatt cttcaaggaa ttcgtgacct ctatcaacac
    10861 caccacaatg tcatcttgcc agacgaagtc ttgaaagcag cggtggatta ttctgttcaa
    10921 tacattcctc aacgtagctt gccagataag gctattgacc ttgtcgatgt aacggctgct
    10981 cacttggcgg ctcaacatcc agtaacagat gtgcatgctg ttgaacgaga aatcgaaacg
    11041 gaaaaagaca agcaagaaaa agcagttgaa gcagaagatt ttgaagcagc tctaaactat
    11101 aaaacacgca ttgcagaatt ggaaaagaaa atcgaaaacc acacagaaga tatgaaagtg
    11161 actgcaagtg tcaacgatgt ggctgaatct gtggaacgaa tgacaggtat cccagtatcg
    11221 caaatgggag cttcagatat cgaacgtttg aaagatatgg ctcatcgctt gcaagacaag
    11281 gtgattggtc aagataaggc cgtagaagct gtagctcgtg ctatccgtcg taaccgtgct
    11341 ggttttgatg aaggaaatcg cccaatcggc agcttcctct ttgtagggtc tactggggtt
    11401 ggtaagacgg agcttgctaa gcaattggca ctcgatatgt ttggaaccca ggatgcgatt
    11461 atccgtttgg atatgtctga atacagtgac cgcacagctg tttctaagct aattggtaca
    11521 acagcaggct atgtgggtta tgatgacaat agcaatacct taacagaacg tgttcgtcgc
    11581 aatccatact ctatcattct cttggatgaa attgaaaagg ctgatcctca agttattacc
    11641 cttctcctcc aagttctaga tgatggtcgt ttgacagatg gtcaaggaaa tacagtaaac
    11701 ttcaagaata ctgtcgttat tgcgacctca aatgctggat ttggctatga agccaatttg
    11761 acagaagatg cggataaacc agaattgatg gaccgtttga aacccttctt ccgtccagaa
    11821 ttcctcaacc gctttaatgc agtcatcgag ttctcacact tgactaagga agacctttct
    11881 aagattgtag atttgatgtt ggctgaagtt aaccaaacct tggctaagaa agacattgac
    11941 ttggtagtca gtcaagcggc taaagattat atcacagaag aaggttacga cgaagtcatg
    12001 ggggttcgtc ctctccgtcg cgtggttgaa caagaaattc gtgataaggt gacagacttc
    12061 cacttggatc atttagatgc taaacatctg gaagcagata tggaagatgg cgttttggtt
    12121 attcgtgaga aagcctaaga cagagttttg aggataaaaa agaaggagcc agctgaaaaa
    12181 aactggttcc ttttgaggta cgacgggcat gtcgtacagt aggttgaaac tagaatagta
    12241 cacatctact tctaaaatat tgttagaaat cgatttgact gtcctgattg atttgtcctg
    12301 ttcttatttc attttactat atatctgagg tgtaagtcct cggtgggtac ctgctaccgg
    12361 tgctcccaat agtgattccc aagcctgact atcgtgaggt agcggattaa aacggtctgg
    12421 ggatagaccg ttttaagtct gacgctggaa ataagaatcg tcagaggaag ggatagcgaa
    12481 atcgtggctc tacgaatagg aacgtgataa taaggcgtat atagcggata aggggcctca
    12541 aactctaaag ttcaaaaagg tagtcgtagc ctatatgcgt aaatcacgag agcaactgaa
    12601 tttgtctgtt cgaggatgga taaactattt ctcattggga aatatgaaaa gtatagtcac
    12661 tagcatagat gagcgcttgc atactcgcct acgagtgatt atctggaagc aatggaagaa
    12721 aaaatcgaga cgattatggg gattgcttaa gttaggggtt cctaaatgga tagcagataa
    12781 ggtatctggc tggtgcgacc actatcaatt agtagctcag aagtcggtac ttaaacgtgc
    12841 tatatcaaaa ccagtcctgg aaaaacgtgg actggtttcg tgtttggatt attaccttga
    12901 acgacatgcg ttaaaagtta gttgaaccgc cgtatgccga atggtacgta cggtggtgtg
    12961 agaggggcta gagattatcc cctactcgat tttaaatcac atgacgttca aaggcatcat
    13021 ctgaaatccc ttgttccaag atgagttttg cccattcttt agcagagaag aggctgtggt
    13081 ccttgtagtt tccgcaagat tcgatggttg tccctgggac atcttcccaa gtagtagttt
    13141 cagcgatttc cttgagcgaa tccttgataa cagctgcgat tttagcactg gtgtgacgtc
    13201 cccacataat catgtggaag cctgtgcggc aaccaaatgg tgaacagtca atcatgccgt
    13261 caatgcgggt acggatgagt ttggctaaga ggtgctcgat agtgtgaagg ccggcagtag
    13321 ggatagagtc ttcgtttggt tgcaccaagc gaatatcata attggagatg atgtctcctt
    13381 ttggtcctgt ttcttcccca atcaagcgaa catagggtgc tttgacaatg gtgtggtcaa
    13441 gttcaaaact ttcgacaata acttcttttg acatggtaaa tcctttcagt tttcttctct
    13501 cattatatca taaaggttgc tcctgagaca gagagaaaac ctctccgagg ctggagaggt
    13561 tgaaatcttt acttacgata taagcggtcg tattggtagt atgggtcaaa ggttacgttg
    13621 atacccagtt tacgaaggac attcttgtct tcatcagtca agatgatggt tgagtgggct
    13681 tcgcttcctt tgaggttgcc gagttcttcc atagcgcggg cagcatcagg attttctgta
    13741 gctgtgatag caagtgcaat caggatttca tttgaatgaa ggcgtggatt gcggctaccg
    13801 agatgatcga ttttaagacc ttggattggc ttaacaactt caggctcgat tagttttact
    13861 tctttagcga tgtcagctga ttttttgatg gcgttgatca aggcagcggc tgtaggacca
    13921 aagagttctg agttcttacc agtgacgatt tccccatttg gcaattcaaa ggctagggct
    13981 ggtccaccag tttcttctgc tttttggcgc gcaacgacag caaccttacg gtctgcaggt
    14041 gtgataccga ggtcgttcat gagcaactca attttcttga cggcagcttc gccaactttt
    14101 tcagctttga agtcaagaac tgtttgatag taacggcgga tgatttcttg tttagaagct
    14161 tcgacagcgg cctcgtcatc tgtaatagcg aaaccaacca tgttgacacc catatctgtc
    14221 ggtgaagcgt atggtgattt tccgagaata cgttccaaca tgcgtttgag cactgggaag
    14281 atttcgatat cacggttgta gttgacagtg gtttctccat aggtttgaag atggaagggg
    14341 tcaatcatgt tgacatcatc aaggtcagct gtggcagctt cataagccaa gttaactgga
    14401 tgatgaaggg gaagattcca aacagggaag gtttcaaatt tagcgtagcc agatttgatg
    14461 ccattgattt ggtcgtggta catattggac atacacgttg ccaattttcc agaaccaggt
    14521 ccaggagcgg ttacgacaat caagttgcga ctggttttga tgtagtcgtt tttgcccatg
    14581 ccttctgggg aaatgatgtg atccatatcc gtcggatatc ctttgattgg ataatgaaga
    14641 taagaatcaa ttccgttttt ctcaagttgg ttgaggaagg catctgcagc gggttggcca
    14701 gcgtattgtg taatgacaac ggaaccaaca aaaatcccta attcattgaa tttgtcaatc
    14761 aaacgaagaa cttcttggtc ataagaaatg cctaagtcgc cacgtgcttt ggaatgttca
    14821 atgttgctag cattaatggc aatcacaacc tcaacctgct ctttcaattc ttgcaagagc
    14881 ttgattttgt tgtcaggttc ataaccagga aggacacgag cagcgtggaa atcttctaac
    14941 attttaccgc caaactctaa gtagagcttg ccgtcaaatt ggttaatgcg ctctaagatg
    15001 tggtcgcgtt gtagatttaa atattgttca gaactaaaag cttgtttttt cattttttta
    15061 cctctggact ctattataat aaaaaattgg aagttaggaa actacggagc taaaaaagaa
    15121 attaaaaata ttaagcaaac gcttgcacaa aattttaaaa agtgctatca tagactatag
    15181 attatgaaaa taatgaggta aacagatgca agaaaaatgg tggcataatg ccgtagtcta
    15241 tcaagtctat ccaaagagtt ttatggatag taatggagat ggagttggtg atttgccagg
    15301 tattaccagt aagttggact atctagctaa gctaggaatc acagcgattt ggctttctcc
    15361 cgtttatgac agccctatgg atgataatgg ctacgatatt gctgattatc aagcgattgc
    15421 ggctattttt ggaaccatgg aggacatgga tcagctgatt gcagaagcta agaagcgtga
    15481 tatccgtatc atcatggact tggtggtcaa tcatacctcg gatgagcatg cctggtttgt
    15541 agaggcctgt gaaaatccta atagccctga gcgagactac tatatctggc gggatgagcc
    15601 taacgattta gattctatct ttagtgggtc tgcttgggaa tacgatgaaa agtcaggtca
    15661 atactatctc cactttttca gcaagaaaca gccggatctc aactgggaaa atgaaaaact
    15721 tcgccagaaa atttatgaga tgatgaactt ctggattgat aagggtattg gtggtttccg
    15781 tatggatgtt attgacatga ttggcaaaat tcctgacgag aaggtagtca ataatggtcc
    15841 tatgctccat ccctatctca aggaaatgaa tcaggcgacc tttggagata aggatctctt
    15901 gacagtaggg gagacttggg gagcaacgcc agagattgcc aagctctatt ctgatccaaa
    15961 ggggcaagaa ttgtctatgg tcttccagtt tgaacatatc ggtcttcagt atcaggaagg
    16021 tcagcctaaa tggcactatc aaaaagagct gaatatcgct aagttaaaag aaatcttcaa
    16081 caaatggcag acagagttag gagttgagga tggctggaat tccctcttct ggaacaacca
    16141 tgacctccct cgtattgtct caatctgggg aaatgaccaa gaataccgcg aaaaatctgc
    16201 caaagccttt gcaatcttgc ttcatcttat gagaggaact ccttatatct accaaggtga
    16261 ggagattggg atgaccaact atccgtttga aacactggat caagtagaag atattgaatc
    16321 tctcaactat gcgcgtgagg ctcttgaaaa aggtgttccg attgaagaaa tcatggacag
    16381 tatccgtgtt attggacgtg acaatgcccg tacccctatg caatgggacg agagcaaaaa
    16441 tgctggtttc tcaacaggtc aaccttggtt ggcggttaat ccaaattacg agatgatcaa
    16501 tgtccaagaa gcgctggcaa atccagattc tattttctat acctatcaaa aactggtcca
    16561 aattcgcaag gagaatagct ggctaattcg agctgacttt gaattgcttg atacggctga
    16621 taaggtcttt gcttatatac gtaaggatgg cgaccgtcgc ttcctagttg tggctaactt
    16681 gtccaatgaa gagcaagact tgacagtaga aggaaaagtc aaatctgtct tgattgaaaa
    16741 caccctagct caagaagtct ttgaaaaaca aatcttagtt ccatgggatg ctttctgtgt
    16801 ggaattacta taaatatttt ttgcagaaaa atttaaaatt gaaatcatat aaaaacaagg
    16861 gaggactgta taaaagacag aaatcctttg ttttttataa ccaaggttta taaactttca
    16921 ttctcgaaat tcaattaact ttacaaattc ccactattaa ggagaaagaa gatgaacata
    16981 aagaagcgtg tccttagtgc aggtctgact tttgcatctg ctttgctttt agctgcttgc
    17041 ggccaatcag gttcagatac aaaaacttac tcatcaacct ttagtggaaa tccaactaca
    17101 tttaactatc tattagacta ttacgctgat aatacagtca attgaaacaa gaacaagaca
    17161 aaagagcctc gtaaaaagta ttgcaacttg gtaatacctt tttgaggtgc tttttgatat
    17221 gagcccatgt tttctcaata ggattgtact caggcgagta gggaggaaga ggtaaaagtt
    17281 tatgcccaaa ctcttcacac aagagttcta gcttccccat tctatggaat cttgcattat
    17341 ccataataat aaccgatggt gtggttaatg ttggtaagag aaacttctga aaccaagctt
    17401 caaaaaagtc gctcgtcatc atctcttcgt aagtcattgg agcgattaac tcaccattca
    17461 tttgttagac ctgcaaccaa agaaatcctc tgatatcttt ttccagatac tttacctctt
    17521 attaactgac cttttaatga gcgaccatat tctcgatgaa aataagtatc gaatcctgtt
    17581 tcatcaatct aaacaggtgc taggtgcttt aaactattaa aattcttaag aaataaggct
    17641 actttttctg ggtcttgttc ataattagat tgaaactaga atagtacacc tctgcttcta
    17701 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcatgttct tatttcattt
    17761 tactatattt ttgtttcgcg ggaagtctac taagatactt aaagatgcag atagtaaaaa
    17821 aaatgtagac attaccgtaa aaaagtgata taatcgtatg atgttcaagg tataggtgtt
    17881 aatcatgagt agacgtttta aaaaatcacg ttcacagaga gtgaagcgaa gtgttaatat
    17941 cgttttgctg actatttatt tattgttagt ttgtttttta ttgttcttaa tctttaagta
    18001 caatatcctt gcttttagat atcttaacct agtggtaact gcgttagtcc tactagttgc
    18061 cttggtaggg ctactcttga ttatctataa aaaagctgaa aagtttacta tttttctgtt
    18121 ggtgttctct atccttgtca gctctgtgtc gctctttgca gtacagcagt ttgttggact
    18181 gaccaatcgt ttaaatgcga cttctaatta ctcagaatat tcaatcagtg tcgctgtttt
    18241 agcagatagt gatatcgaaa atgttacgca actgacgagt gtgacagcac cgactgggac
    18301 tgataatgaa aatattcaaa aactactagc tgatattaag tcaagtcaga ataccgattt
    18361 gacggtcaac cagagttcgt cttacttggc aacttacaag agtttgattg caggggagac
    18421 taaggccatt gtcctaaata gtgtctttga aaacatcatc gagtcagagt atccagacta
    18481 cgcatcgaag ataaaaaaga tttataccaa gggattcact aaaaaagtag aagctcctaa
    18541 gacgtctaag aatcagtctt tcaatatcta tgttagtgga attgacacct atggtcctat
    18601 tagttcggtg tcgcgatcag atgtcaatat cctgatgact gtcaatcgag ataccaagaa
    18661 aatcctcttg accacaacgc cacgtgatgc ctatgtacca atcgcagatg gtggaaataa
    18721 tcaaaaagat aaattaaccc atgcaggcat ttatggagtt gattcgtcca ttcacacctt
    18781 agaaaatctc tatggagtgg atatcaatta ctatgtgcga ttgaacttca cttctttctt
    18841 gaaaatgatt gacttattgg gaggggtaga tgttcataat gatcaagagt tttcagctct
    18901 acatgggaag ttccatttcc cagtagggaa tgtccatcta gactctgagc aggctctagg
    18961 ttttgtacgt gaacgctact cactagccga tggagaccgt gaccgtggtc gcaaccaaca
    19021 aaaggtcatt gtagcaatta ttcagaagtt aacttctaca gaggttttga aaaactatag
    19081 tagtattctt caaggattgc aggattctct tcaaacaaat atgccgattg agactatgat
    19141 agatttagtg aatactcagt tggaaagtgg ggggaattat aaagtaaatt ctcaagattt
    19201 aaaagggaca ggtcggatgg atcttccttc ttatgcaatg ccagacagta acctctatgt
    19261 gatggaaata gatgatagta gtttagctgt agttaaagca gttatacagg atgtgatgga
    19321 gggtagatga aatgatagac atccattcgc atatcgtttt tgatgtagat gacggtccca
    19381 agtcaagaga ggaaagcaag gctctcttgg cagaatccta caggcagggg gtgcgaacca
    19441 ttgtctctac ctctcaccgt cgcaagggca tgtttgaaac tccggaagag aagatagcag
    19501 aaaactttct tcaggttcgg gaaattgcaa aagaagtggc agatgattta gtcattgctt
    19561 atggcgcaga gatatactat actctggatg ctctagaaaa gctagaaaaa aaagaaattc
    19621 ctacccttaa tgatagtcgt tatgccttga ttgagtttag catgcatact tcctatcgtc
    19681 agattcatac gggattgagc aatattttga tgttgggaat cacgccagta attgctcata
    19741 ttgaacgtta tgatgcttta gagaataacg aaaaacgtgt tcgtgaactg attgatatgg
    19801 ggtgctatac tcagataaat agttatcatg tttcaaaacc taagttcttt ggtgaaaaat
    19861 ataaattcat gaaaaagaga gctcggtatt ttttggaacg tgatttagtt catgtagttg
    19921 caagtgacat gcacaattta gacagtagac ctccatatat gcaacaggca tatgatatca
    19981 ttgctaagaa atatggagcg aaaaaagtaa aagaactttt tgtagataat cccagaaaaa
    20041 ttataatgga tcaattaatt taggagaaaa tatgaaggaa caaaacactt tggaaatcga
    20101 tgtattgcaa ctattcagag ctttatggaa aagaaagttg gtcattttat tagtggcaat
    20161 tataacttct tcagttgctt ttgcctacag tacttttgtt atcaaacctg agtttactag
    20221 tatgactcgg atttatgtag ttaaccgtaa tcagggagag aagtctggtt taaccaatca
    20281 agacttgcag gcaggatcat ccttggttaa agactatcgt gaaattatcc tatcgcagga
    20341 tgttttggag gaagttgttt ctgatttgaa actagatttg acgccaaaag atttggctaa
    20401 taaaattaaa gtaacagtac cagttgatac ccgtattgtc tctgtttcag ttagtgatcg
    20461 agttcctgaa gaggcaagcc gtatcgctaa ctctttgaga gaagtagctg ctcaaaaaat
    20521 tatcagtatt actcgtgttt ctgatgtgac aacactggag gaggcaagac cggcgacatc
    20581 accgtcttcg ccaaatatta aacgcagtac actaattggt tttttggcag gagtgattgg
    20641 aactagtgtt atagttctta ttcttgaact tttggacact cgtgtgaaac gtccgaaaga
    20701 tatcgaagat acactgcata tgacactttt gggaattgta ccaaacttga ataagttgaa
    20761 ataggagaga ggaatgccga cattagaaat agcacaaaaa aaactggagt tcgttaagaa
    20821 ggcagaagaa tattacaatg ccttgtgtac aaatatacag ttgagcggag ataaactaaa
    20881 agtaatttcc gttacttctg ttaaccctga ggaaggaaaa acaactactt ccgtaaatat
    20941 agcaaggtcg tttgcgcgtg caggctataa aactcttttg atcgatggcg atactcgaaa
    21001 ttcagttatg tcaggatttt ttaaatctcg tgaaaaaatt acagggctaa cggaattttt
    21061 atctgggaca gctgatttat ctcacggttt atgtgataca aatattgaaa atttatttgt
    21121 agttcaatcg ggcactgtat caccaaaccc tacagccttg ttacaaagta aaaattttaa
    21181 tgatatgatt gaaacattgc gtaaatattt tgattatatc attgttgata cagcacctat
    21241 tggaattgtt attgatgcgg caattatcac tcaaaagtgt gatgcgtcca tcttggtaac
    21301 agcaacaggt gaggtgaata aacgtgatgt ccaaaaagcg aaacaacaat tagaacaaac
    21361 agggaaactg ttcctaggag ttgttttgaa taaattagat atctcggttg ataagtatgg
    21421 agtttacggt ttctatggaa attatggtaa aaaaataatt taggaaagat tctatggatg
    21481 aaaaaggatt gaaaattttt ttggcagtat tacagagtat tattgtcatt ttattggttt
    21541 attttcttag ctttgttaga gagacagaac ttgaacgttc ttcgatggtt atactatacc
    21601 ttctccactt ttttgtattc tattttagtt cctatggtaa caattttttt aaaagagg
//
LOCUS       DAVHIT010000030        21395 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVHIT010000030 DAVHIT010000000
VERSION     DAVHIT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.030"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            228..654
                     /locus_tag="VVN16_000983"
                     /pseudo
     CDS             228..654
                     /locus_tag="VVN16_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            725..883
                     /locus_tag="VVN16_000984"
     CDS             725..883
                     /locus_tag="VVN16_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU5572395.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(1251..2978)
                     /gene="ezrA"
                     /locus_tag="VVN16_000985"
     CDS             complement(1251..2978)
                     /gene="ezrA"
                     /locus_tag="VVN16_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU5572396.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(3060..5006)
                     /gene="gyrB"
                     /locus_tag="VVN16_000986"
     CDS             complement(3060..5006)
                     /gene="gyrB"
                     /locus_tag="VVN16_000986"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU5572397.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(5021..5584)
                     /locus_tag="VVN16_000987"
     CDS             complement(5021..5584)
                     /locus_tag="VVN16_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5572398.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(5669..6223)
                     /locus_tag="VVN16_000988"
     CDS             complement(5669..6223)
                     /locus_tag="VVN16_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU5572399.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(6234..7457)
                     /locus_tag="VVN16_000989"
     CDS             complement(6234..7457)
                     /locus_tag="VVN16_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5572400.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(7494..9944)
                     /locus_tag="VVN16_000990"
     CDS             complement(7494..9944)
                     /locus_tag="VVN16_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU5572401.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQKNDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(10144..11073)
                     /locus_tag="VVN16_000991"
     CDS             complement(10144..11073)
                     /locus_tag="VVN16_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU5572402.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            11213..11500
                     /locus_tag="VVN16_000992"
     CDS             11213..11500
                     /locus_tag="VVN16_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU5572403.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFSAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(11534..12868)
                     /gene="ciaH"
                     /locus_tag="VVN16_000993"
     CDS             complement(11534..12868)
                     /gene="ciaH"
                     /locus_tag="VVN16_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU5572404.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12858..13532)
                     /gene="ciaR"
                     /locus_tag="VVN16_000994"
     CDS             complement(12858..13532)
                     /gene="ciaR"
                     /locus_tag="VVN16_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU5572405.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(13641..16187)
                     /locus_tag="VVN16_000995"
     CDS             complement(13641..16187)
                     /locus_tag="VVN16_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5572406.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(16310..16750)
                     /locus_tag="VVN16_000996"
     CDS             complement(16310..16750)
                     /locus_tag="VVN16_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU5572407.1"
                     /translation="MTPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(16725..17594)
                     /locus_tag="VVN16_000997"
     CDS             complement(16725..17594)
                     /locus_tag="VVN16_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU5572408.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(17572..18072)
                     /locus_tag="VVN16_000998"
     CDS             complement(17572..18072)
                     /locus_tag="VVN16_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5572409.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(18083..18781)
                     /locus_tag="VVN16_000999"
     CDS             complement(18083..18781)
                     /locus_tag="VVN16_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU5572410.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18774..19040)
                     /locus_tag="VVN16_001000"
     CDS             complement(18774..19040)
                     /locus_tag="VVN16_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU5572411.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19106..20032)
                     /locus_tag="VVN16_001001"
     CDS             complement(19106..20032)
                     /locus_tag="VVN16_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU5572412.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20107..20922)
                     /locus_tag="VVN16_001002"
     CDS             complement(20107..20922)
                     /locus_tag="VVN16_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572413.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20926..21246)
                     /locus_tag="VVN16_001003"
     CDS             complement(20926..21246)
                     /locus_tag="VVN16_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5572414.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6452 a         4650 c         3849 g         6444 t
ORIGIN      
        1 ttgaacttac aatcgaaaaa tggttaagta aaagtcaaca gttactatag tttgcaaatt
       61 atttacagaa agaacaaaaa ttcctaggag attattctgg ctatgtgcat tgtgatatgt
      121 tgcggcagta acttaggact ttagtcctct agttctgcct atgcgatagc agtccaaggt
      181 ttaggagcaa ggcgacgcta agcttggtaa actacgaacc gctagaagct tatcgtcaac
      241 tggaagaagc tgcacttgtt ggatgttggg cgcatgtgag aaggaagttt tttgaagcgc
      301 cccccaagca agcggataaa tcatcgttag gagctaaagg tttagcttat tgtgatcagt
      361 tatttgcctt ggaaagagac tgggaggaca ttgtcagctg atgaacgact acagaaacgt
      421 caagaagagc tccaacccct aatggaagac ttctttgctt ggtgccgtcg tcagtcagtt
      481 ttagcaggtt caaaactagg aagggcaatt gaatacagcc tcaagtataa agaaaccttt
      541 aagactattt tgaaagacgg acatctggtc ctttccaata atctagctga atgcgccatt
      601 aaatcattgg ttatgggacg gagtaaaaga gtccagtgga ctcttttagc ctaagctcag
      661 tttaaaaaag cgagggtggt tgttttctca aagttttgaa ggagctaaag caagagctat
      721 tattatgagc ttattggaaa cagctaaacg tcatcaacta aatagtgaga aatatctatc
      781 ctatcttcta gaatgtcttc caaacgagga aactctcgta aacaaagagg ttttagaggc
      841 ctatttaccg tggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg
      901 aactatccgt gagttctcta gtctggagat ttttcaatag acttcgttat tggacagtta
      961 ccaagttact caaagtacag atttgaagct gcagataaga ctgacgaaat cactttaata
     1021 ctaaatgaaa atcaaaaagc aaactaagag cctcaggctg tacttgagta cggtaaggcg
     1081 acgctgacgt gatttgaaga gattttcgaa gagtataaaa cactgttttg aggtggtgga
     1141 taaatcggct gggcaaaaac tcaatctcag gagaaaatga agtaaatctc cccacaacta
     1201 tagaaaaaga tttgattctg ctcctcacac aataaaatct ttttctttta ttaaaaacga
     1261 atcgtttcac gtgttttctc atatgaggta acaaagcgat tggttacacc aggctctgcc
     1321 acttccaatg cttgagaaat cttgtcaaat gaagcgtgat aatcaaattc tttttcaaaa
     1381 atatctaaag cttcgttaaa tgcttcttga atgcgttcat caaatgagcg atagcggtta
     1441 gaatattgca agagttgctc tgtcaaagtt gcatattgta caatattata agtttccgtt
     1501 tctaaagctt ccatatcatt cgttgcaatt tcaagaactc gggtaacaga ttcaatgtta
     1561 atcatttttt gttctaactc aaccattaaa tcctcggtat tattgcttgc cgtaaagaat
     1621 aacttcaaga aagtttgtgg aatacctggc agattgcgtt tttccatgta tcgcttgata
     1681 gtatggagac gattgacata aacattggcc ttttgacgtg cattaatatc atctttctca
     1741 atttgtgtca ggcgctcact aactgaaatt tgctcatctt caatatcttt tagttgagtt
     1801 tgtaaatcct caagattttc ttcaagaact gaataagctt gggttggttc ttcttgattt
     1861 gaagttacct caacaatagc tgcctcaaaa ctctctaatt ctgtctgaat acgacgaaca
     1921 tggcttgcag ctgtctcagg aagtaaatag gtcttgttca aacgtgcaat atcttctccc
     1981 aataaagtat tattctcttt catatgttga aggtaagttg gaagagttgc aagtagattt
     2041 tccactactt tctgagcagc aatttctcga gtaaaaatat catacaaggc attgatttcc
     2101 tcttgtgcct gtccattctc atattcggca ttatccaatt ccaactgacg aatattctct
     2161 tggtttttct tgaatgcttc ataaagcaag tggaaacgcg cttcaatatc cgtttcaaca
     2221 aaatgataat tagcatcaat tagtttacga taaccggctt ccaaatcctg taattgatct
     2281 ggcaattctg tagaaagcgt cgtaaccaag gctggaacac gatccacaat atgacttaag
     2341 gccaaaatgt gattttctgt attatccaaa atcactgcgg cttccacagg gtcacccgat
     2401 gaattcaagg ttacaaattg tgaaaattca gattggatat tttctaattg tttttcaatt
     2461 tcatccaagg cttgaccata ctgttctgaa ttttcagcaa ctctatgctg aagttcctca
     2521 aataaatcca aagcatgaag aacacgacca ctatttttag attcttgctt ctctaagtct
     2581 gccaaagcat tgcgaattgc cgcaatatct tcttcaatca aagtaatttg actctcaatt
     2641 tggtcaattt gatgactggc cttgagaaaa cgaaatgaat ggttatagcc ttctgcttca
     2701 aagagattat tttcaatatc ggcaaaagag ttgagagata aatcgaccca tttttgattc
     2761 cattcacgga aagccacttg actttgtcca atcaagtgca tattttttac agcttctact
     2821 tcatcattta ctggaagatt gtatagttct tcttttcttt cttctagcgc ctctaatctc
     2881 ccctcgtttc gcttacgtag aaagattgcc actacataag ccagaactaa aatgactgca
     2941 attgcaacca ttaaataaat tagttgtcca ttagacatat caaactcctt ttttacttga
     3001 aacaatcgta acgattatat catatttttt agaccaaggg aactacttct ccctattttt
     3061 tagacatcaa gtgtactata gacagcattt tcttcgataa actcacgacg aggctctact
     3121 cgatccccca tcaacatatc aaagatttta tctgcttctg cagcatcatc tacagaaact
     3181 ctagccatca agcgatgttc gggatccatg gttgtttccc acagctgatg atcgtccatt
     3241 tcacctagcc ccttataacg ctgaatagtc ggtttggtac gaccttcact ataacgggct
     3301 aaagcttctt ggagtttgat ttcttgatct gcacccggct ggatatattc tttaatctcg
     3361 cttccaacct tgacaccata gattggtggt tgggcaatat aaacataacc agcttctagg
     3421 attggtttca tataacgata aatcaaggtt aaaagaaggg tacgaatatg ggctccatcg
     3481 acatcggcat cggtcatcaa aacgagtttt tggtaacggg ctttcgaaac atcaaattct
     3541 gcgccaaatc ctgttcccat ggctgtgaaa agactacgaa tttcttcgtt agctagaatc
     3601 ttatccatac ttgctttttc aacgttcaaa atcttaccgc gaattggaag gatagcctga
     3661 aactcacggt tacgaccaga tttggctgat ccaccagctg agtctccttc gacgatgaag
     3721 agttctgttt cagcagggtt attagaagaa cagtctgcta gtttccctgg aaggttggaa
     3781 atttccaaac cagatttttt acgtgtgact tcacgcgcac gcttggcagc cacacgagcc
     3841 ttggcagcta aaatcccttt ttccacgata cgtttggcaa tctgtggatt ttccatgagg
     3901 aaatcggaga aggcttcact gaagaggcga ttggtaatct tgaccacttc gctatttccc
     3961 aatttggtct tggtttgtcc ttcaaactgt ggatttgggt gtttaactga gataactgca
     4021 gttaagcctt cgcgaacatc ttcccctgtt aaattatctt cattgtcttt cagtaactta
     4081 ttcttacgag cataatcatt gataacacga gtcagtgccg tacggaaacc ttgttcatgc
     4141 gttccacctt catgggtatg aatattattg gcgaaactca tgacattttc atggtaacct
     4201 gttgtgtact gcatggctac ctcaactgtg atatcatcca tctcaccgtc tgtatagatt
     4261 ggtgtatcaa agattacatc cttgttctcg ttgatatatt caacgtaact agcaatccca
     4321 ccttcataat gataatgctt ggtttgttcc aaaccttggc gcttatcagt gatagaaatt
     4381 tgaagaccgc gatttagaaa ggccaactct tgaatccgtt tatttaattt atcaaaatca
     4441 aaggttgttg tttcagtaaa gatttttggg tccggtatga agtgaaccgt tgttccagtt
     4501 ctatctgtat ccccaaccac ctcaaggtct gcaacaacat gaccacgacg gtattcttgg
     4561 taatgaatct taccgttttt atggacatga acgtctaatt gagtggaaag ggcattaact
     4621 actgacgacc ccaccccgtg aagaccacct gaaaccttgt atccaccacc gccgaacttt
     4681 cctccagcgt gaaggactgt aaagacggtc tcaacagcag gacggcctgt tttttcctga
     4741 atatcgactg ggataccacg cccatcatcc acaacagtaa tcgaatcatc tggctcaata
     4801 aaaacttgaa tatggctggc aaatcctgcc aaggcctcgt caattgagtt atcaacaatt
     4861 tcccagacta gatggtgaag accttctttt gaggttgatc caatgtacat ccctggacgc
     4921 atacgaacag cctctaagcc ctctaaaact tgaatttgac tggcatcata atcctgtgcc
     4981 tgcagatttt tgatttcttc tgtcatctta ttcctttttc ttatatgtct aatacttgtc
     5041 ttaaattcac gatactggta aacaagtggg tatcaagtcc tgcagcttga cctgcttcga
     5101 tatcaatcgg ccgatcacca atgacaagac cagagctaat ctgatacttt tctcttaaat
     5161 aaagcatgga ttcgggattt ggctttctct taaagcctga gctagaagtc accacttctg
     5221 taaaataagc tgctatagag gttttttcta aaatttccaa aacctgatca tttcgatgag
     5281 agaccaaaaa atgacggcca ccttgatttg aaatgtcttc caataggtca gaaactcctt
     5341 caaataaaat cgggtgttca agctctctgg cttcattttc cttgtacttt tctaaaaaat
     5401 tctctaaatt gggagcgaat gtctcaatcg caaaaggagt agaaaccttt aaagcttgat
     5461 agacactgtc atggtcttgt gtgataccat acagtgccaa tgtttcaaca aatgcagctg
     5521 ttgaagtttc ataattatcc agtaaagttc cacctaaatc ccagatgtaa tcgtgatatt
     5581 tcataccctt cattatagca tttttttatg aaaaaagcga tgatttccct gagttttcca
     5641 cataaaaaac gagagaataa ctcccgtttt actgattttt accaaagaca tcttgataga
     5701 gcattcctgt tcgtaaactc tctgcgtccc ctcctagttg ctccaccaac tcgtaggcaa
     5761 aggcaagggc tgttgaagga ccccgactgg ttgtcaactg accatctacc actactgttt
     5821 ccttgacgta gtgaccatca aggatttgct cttgaacgcc gtcataacaa gtgtatcgct
     5881 tatttttcaa tatctctgct tgattgaggg caattggtgc cgcacaaatg gctgctagtt
     5941 tcttcccttc ttgctcgaag ctttgcaatt cttgaatcaa ggtctgatta tcacgtaaat
     6001 gtgcagaacc aggcatacct ccaggaagaa caatcatatc atagtctgat aaatctccat
     6061 caaagacatg atctgctctt acttggattg catgcgaacc cgttacttgc tcttcaaaac
     6121 caaccatatc acatgtgata ttggctcgac gcaagacatc tacaactgtc aaggcttcaa
     6181 tttcttcaaa gccctgagct aacataactg ctacttttac catgattttc tccttattta
     6241 atttgtttta atacaacctt tttccgtttg aatgggactt ttccgctctt ttcttcagct
     6301 agattagtct ggtaactcat cgataaaagc aaaccaacac caatcagatt actgataata
     6361 gctgatcccc cttgcgaaat gaaaggcaag ggaatccccg tcaaaggaag tagtccagtc
     6421 acagcaccga tattctcaaa gatgtggaag agcaacatca taatcaaacc tgtggaaata
     6481 taagtgtaga actggttatt tgatttaaga gtaatcttca acatacggta aatcaacatg
     6541 agatagaggg caataaccag gacagagcca ataaagccaa aatcttctgc aataaccgta
     6601 aaaatcatat ctgactctcg aactgggata agcagattcg aagcattaaa tccctgacca
     6661 aataagccac cactcccaat ggcaatctgc ccttgagcct gctggtaagt cgttgtttgg
     6721 gcaaactcaa agggattgag ccaagccaaa atccgattga tttggtaggt cggcattcca
     6781 atctggtgaa gaaaagctcg tccgtccttg ctaataaaga tagctaagaa accagcaact
     6841 cctgttacag cagtcacaaa tactgggata ataattttcc aagaaacccc tgataataaa
     6901 acgattcctg agaaaatggc tacaaaaacc aaagccgtcc ccaagtcact ttgaagtgct
     6961 aaaagaacta ggactggaat ggtaaagaga atcatccaga aaattaacaa aaagtccagc
     7021 ggaaccgtgc gtctccattc cttatgtttc tttgtaaatt ggacaatgac acgagccaac
     7081 atgaggatat aggatatctt cataaattct gacggttgaa atagggtaat tccatttatt
     7141 gatacccagt ttttggcacc cgttgatgca actaagcttg gattataaaa tacaatcggc
     7201 aagatcataa gtcccaagcc taaaatatat agaaaggggg tcaccttcca aagaaattct
     7261 gtattaaaga gcatgaccac aaaaccaatc acaagcccca aggcaatcca ggcgacctgc
     7321 tgccctaaaa tgggcagaat attattggga taatcatgac taacggctat atagatagcc
     7381 accacaccga tgaccagtag aaaaaatact ggcaagagca aactataatc gactctagag
     7441 tcgagagaac gtttcatata tactaacctt atactttcat acaatactat ttatcaaagt
     7501 tcattaaaaa atctatcaat agcctcgtca acttcggatc gagagatggt tttaacagtt
     7561 gcttcttctg ctagagatgc tactatttgt ttgccgtatc gttttccgat gattctccta
     7621 tccaaaataa gagttaagga acgttggtat tcacgtctca tacttcttcc caaagcctgt
     7681 tttaaacgaa taatggccat tggcaattga taatcataaa aggcattttt cccttcttga
     7741 ttcagttctt gattaatctt tttcgtcaag ggttcttgag gattttggaa aggaagcctc
     7801 ggtacaactt gaatcacaaa aggatggctt gaaaaatcaa ctccctccca gaaacttgct
     7861 gcaccaagca agatttgttg ttcacctttt tcaaagcgtt tctttagctg atgaacatcc
     7921 ccatttttat actgggccaa gtggctaact gtaagtaaat ccgatactgc tagaagcatg
     7981 tctttagcgg taaagagaac caaaatcggt tgctggaaag cttgaatttc cactagtaaa
     8041 gcagctacct ctctggcata gacttctaag gaggtttctg ttaccagagg gaaatctttg
     8101 accataacca cttcttgttc ctgttttccc cgagattcaa tcttgacaaa tttggcttca
     8161 ggatagccta aaaggtctgc caaagaaacc ctctgactaa tctcaagagt agccgatact
     8221 cccaagactt ggcaactctc aggaagtaaa gaggacagaa gagtacggca tgattttgta
     8281 gaagaaatct gaattttctt ttgactcgtt tcagttactt caagccagta gtcaccttca
     8341 gctgtaaaat agcgaaccag ctcatcaaag ccttctactt ctaattctga aaaatactga
     8401 tggagattgt ccagagaatc taagatattt ttcctagatt taccagactg aaattgttct
     8461 atcaagtaga gacactcaaa gcgaatactt tctagtatcc gttgttgaac cctgttttct
     8521 tctcctacta aagccttatc aattaaatcg ataatagatt gtatatcgta ggtctcttga
     8581 agcagatttt ctagagctaa caaaatcttt tggacttcat caataatcag taaacggtca
     8641 ctgacaaatt caggattatc ttcaagtctg gtcacgagat aggcatgatt agtcactaaa
     8701 agcttgcaag tctctgccct ttcttgacta cgtttccaaa aatcttccgt cacaaataag
     8761 ctctgggatg ataaattccc atcatgacga aggtctgcta gaaaatgttg gtaacggtag
     8821 agttgcccga tttcatccaa atctcctgtc tctgtctcag taagccagac caagacttgc
     8881 attttaaagc gtctaaataa gcgattttca tcatttttct gcaaggaatg ataaaaggca
     8941 tccaacttca gataattttg tggtcccttt aagctatgaa tatctgtatg gaacacttcc
     9001 ttgaggcgtt taccttcttc ttccatgatt tgattttgaa gaatctttgt cggaacacta
     9061 agaacaattt gtcgctcttt ggattgagat aaagcgggta agagatagcc ataggttttc
     9121 ccaatccctg tcggcgcttg aatcagagag acaggttcat ctttcaatag caagccaacc
     9181 tctttagcaa aactttcttg ttcctccctc acttcaaggt tcaacagaga aatattttta
     9241 gaaaagtctt gagatagttt tcgtggctcc agagaagctg ccgttttctt aaaatacaga
     9301 ccttgaactt ggaccaagtc tggagaactc aggatagatt ggttgcgata agtttcctca
     9361 ataaccaggt aggactcata taggagagcg tcagccattt ccagcaagcg ttccaagaga
     9421 cctttaggaa gctgggtcat cttttcccgt aaaaaaagaa gtaattctgc tgtagcttgg
     9481 gcatctgaaa gggctgtgtg tgcgtgttta agaggaattc ctaattctcg acacaaaatc
     9541 ggcaagctat atttttccag ttcagggaaa aagacctggg ccaattcgac cgtatcaaca
     9601 cgagggtttc ttagctcata gccttcaaaa aataaatttt ccgccaagag attagcatca
     9661 aactgaacat tatgggctac aaaaatccca tcctccacca agtcaaatat ttttctggca
     9721 acttgcgaaa aatcaggtgc ttgcgccaga cgttggtctg tcaatcctgt cagttctttg
     9781 atatgagcat ccaagggttc atgtggattg acatccgtcg tatagtgatc gacgatttct
     9841 ccgtcctcaa tcacgacaat tcccacttgg ataattttag ccttactacc tgtgctagtt
     9901 gcctctaaat ccaccacaac atagcgatta ccaatcgttt tcattataaa aaattatacc
     9961 aaaaaaaggg ccatttggca cctgcaaaca taggataatt ttcaaactca agaacactac
    10021 ttttcttaaa aatcccatcg aatagagcat tctagcctgc tgatttttgt aaaatagtga
    10081 agaaatacga aaaattgaga gaagaaatgt ttgcatcttc cttctctcaa cgaatcatgt
    10141 attttatcta accatgtcag gatcgtagtc ataaaatcct gtatcaagga aacggttttt
    10201 agggtgaagt ttacgaactt cttcatccag caagaaggct tgatcatggc taaagttgcc
    10261 ctcttgaatc aagttacgcg cttggataaa gagttttgca atctcaacaa agttctgacc
    10321 aggagtgtag agcccttcta gtttccagcg agtaaagcca tgctccacca attctgttaa
    10381 tttgatcatc aaatcaaggt cattgttggc aaagatatgg gtcccatgat tatcttcaaa
    10441 aatggaatag tggctctctg gatcacttgg ctcagccaag aagaggtcac gtttatgcgt
    10501 cttttcatca tcgatatgtg taaagttata gtagttttgc aagagtggac gtttagaatg
    10561 atggatgacg ctagcaccgt aaaccaaaac ttcagcagga atttccaaaa tctctggcat
    10621 tttgaaaagt tcagctgatg gaatttcacg cgccaaaaca gcctcagatg cgccagcctt
    10681 ttgtccccag aagttaatct gacgactgct agttaccatg gttgaagcat cgtagatggt
    10741 cttaaatgaa taaccatcgc ggttaactac gtaaaagacg cctgcatccc caatcgtaat
    10801 atagtctgtc ttgatttctt ccaagaagtc taagaaaggc ttgatacggt ccatcatatc
    10861 ttggtgcatg agagcattga ccgcaacgat caattcctta ccagcatcat gaaccaactt
    10921 agcgatttca cgtaattggt cataactaaa ggtcgttggc agacgaaggc caaaatcttt
    10981 ctcaccgaca tagatacggt ctacgccagc ttcgagtagt tgttcaactt gttcaatact
    11041 ttcagcagtt gctgtaatga caatcttttc cataagaaaa attataccac atttctccaa
    11101 aatcatccat gctttgaaaa tgaaaaggga ctttattctt tttgtaacct ttctgtaata
    11161 attctctgcc gaaaaaatga taaaataggt atgtactgtt aaggagagaa ttatgcccgt
    11221 aagaaaatta caatcctatg aggtagacta tcaagaagaa ttaaaccagc agcttcctca
    11281 ttaccaagct tatacacctg aagcgcaatc tgatgccaat ctcaaggaaa tcctattttt
    11341 tattaatatc gctgtttttt gtatctgtat tgctatcttt agttttatct ttttagcatt
    11401 aaaattatca actgctcttg ccttttccgc agcaatcgga ttcagcttac ttgttttaaa
    11461 agttcaacgg tctattatca aacgaaaacg tagaagataa atccaaaata tcgccccagt
    11521 tggagcgata tttttatttt ttctttttag atggtgtctg aatggcaatc ttcacttcaa
    11581 agattgttcc cttgggttta ttatctttga cagtaacagt tccttttaga gcatctacaa
    11641 tttgcttggc tagggataat cctaaaccaa aaccaccttt ttgccgggtt ctagctttgt
    11701 ctactcgata aaaacggtca aaaattttct ttttatcttc tgtcgaaata ccgattccat
    11761 tatcagaaac aagtaaataa agattgcgat cggtcgccga gataagaaaa tcaatttcac
    11821 catcctcctc agtatacttg acggcattat cgaaaagaat ggtcatcagt tgtttcagaa
    11881 gaagctgatc tgtgacaatt gttcgatgga tacgattttc aaaacggaag acacgattat
    11941 tttccgaagc aatcatctcg tagtttgtga aagttgtatt aaaaaagcta gttggaactt
    12001 ctgcaagctc cggcttaatc ccatcatctc tccgagctaa gttcagcaag cttgtcgtta
    12061 aaaaacgcat atttcggact tcttccaaac tcgatgcaat gctttcgctc acatccataa
    12121 tggtagcttc tggcttacga aaaagggtct ctaagcgatt ttgcaaaact gcgagtggag
    12181 ttcgtaactc atgactggca ttttccacaa aagactgttg cttctgcata ctctcaagca
    12241 ggggcctaac actgacccta gctagataga gactggcaag taaagacaaa atccagaaac
    12301 tagccatcac gaccacaatc aattgctcat gcttttgact agcctgctcc aactgactgg
    12361 tattaatcaa gacagcagca tacttgatat tggttgaaac cgaactaata ttggtctcca
    12421 tcaaaatcac acgatagatt tcttcctgcc catagctatt aaaaacctga atctggtaga
    12481 tatgtcctag ttctttcttt tctaacttaa tcttatccaa gcccaaaaat cgatttccag
    12541 aaagaagttg agtaaagtct ttatcaaaga gaatgacttc cgtattggaa ctgacattag
    12601 gttttatttc tactttacta gcgtccgccc tagcattttc taaatcttta atctcttctg
    12661 ttgccctatt tatagccagc tgaataactg cttgaggatt ttcactcagt ccatgaagct
    12721 tatcgtccac cgaagtataa agactcgaat gcatgacttg taaaataatc agagtcattg
    12781 tagaaaaaat cagggtgaag acaccgaagt tgcggataaa ataactaaag tcatccgcat
    12841 accatgtttt tttaagttta ctgaacatct tttaaaagat acccaacact acgcaaggtt
    12901 tgcaaattct ctgcaaaagt ggttcccttt aatttcttac ggacttttga aacatagact
    12961 tcgacaaccg aaatcgttgt atcactatca aatccccata gacggtcaaa aatctgcgtc
    13021 ttaggcaaaa tcacattttg attttgaagg aaataaacta gtaaatcgaa ctctttcccc
    13081 agcaattcga caggagtatc ttcaacttta acggtattgg ttgataaatt aaccacgata
    13141 ttcccataag tcaaggtgtt ttcattaaac ttccctgaac gtttgagaag ggcctgaatc
    13201 cgcattttaa gttcttctag gtagaaaggt ttggtcagat aatcatccgc tcccagttca
    13261 aatccatgtc ccttgtcatc caaactttcc ttggcagtca taatcagaac tggtgtcgta
    13321 attccctttt cacgcaattc ttttaagact tggaaaccat ttttttctgg caacatcaaa
    13381 tccagcaaaa tcaagtcata gacaccactc tcagcttcgt agagaccttc ttctccatca
    13441 aatacctgca taacatccgc aaaatcgtct aaaaagtcaa atactgaatt tgacagacct
    13501 aggtcatcct caaccaataa gatttttatc atgagaaact cctccttatt aaaactatta
    13561 taccaaattt gccttaaaaa aaactcaact ctctgcattt tacatgagat agctgagttt
    13621 tctttttatt ttaggcttat ttatgcattt ccgtattgaa gaacaactgc ttcgactgca
    13681 gctttttcac ggctaatcaa gtcaacacgc gctgcaattt ccttgattcc cataccgatg
    13741 ttacggctaa gagcaaggtc agaaagttgc ggttcaaaga actccttgta ttccgccaag
    13801 cgttgctgag tcttaaatac atgagcagga aggataacaa agctatcaaa gctcatatct
    13861 cctccaaggg ctgccttaat ccaagcccag ttttcacgcg cccaagacca agctgttttc
    13921 tgagttgctt gatgagctag gaattggtaa taccaagcag acaagtcctg tggtttgacc
    13981 acaaatttgt ccttccaaga agtaatcaag ttttggatat tatccgcatc tgtactgtat
    14041 gcaagagctg ctgccaactg gcgtttaaag acagcatctg ttgcgtgagt ataagtatca
    14101 agataaagtg ctaacaagtc tttagtctca tgatgtttca tctcattaat cagaacttgt
    14161 gaacgaatag ctgctgggag tcctgcaaga ttctccttgt gtgttgcgaa gatttggcta
    14221 gcgacttgac tagcttctgc atcatttgag cgaatcatca tcgaaacagc cagctgacga
    14281 accaattcat cctcatctga ttctccgtct ttagcttcaa aaccaagacg gtcatagtta
    14341 tgacgagcca atttagcaac cagtcctttg aaggctgttt cagcatccgt tccttcatca
    14401 ataaagcgct caagggctga aatcacttga gaaacagctg aaaccaccag ataagactct
    14461 tccttagcaa gtttatcaag aactggaagc aagtctgcat aagaaatgtg ccctgcctca
    14521 gccaacaaac gacgttcttg aacaatttgc agtttgcttg tgttatcaag tgtctctagc
    14581 tcagcaagaa cagcttctaa caagtctcct tgatagtcgg taatatagtg ggcagtattt
    14641 tcagtgttga gacgaagagc tccttcattt tcagcaagaa gagctgcgta gccagggatt
    14701 tcgatacttt cagtttcgag tgtatcaggc aagcctttcc agttgctatt gaggggcacc
    14761 acccagagac ggttcttgtc ttcgttctca ccgatgaaga attgtttttg tgaaatcttc
    14821 aagacatcat tttcaacttt gacagtaaga actgggtaac caggctgttc caaccaagaa
    14881 tccatgaagg ctgcgacatc acgtcctgac gcttgaccaa gggcatccca aaggtcacta
    14941 ccaatggtat tgctgtattg gtgtttttca aagtaggcgt gcaaaccttt agcaaaatca
    15001 gcatctccta gccaacggcg aagcatgtgc atgagacggc ttcctttggc atagacgata
    15061 gcgccgtcaa agagtgtatt gatttcatct ggatgtttaa cttcgacgtg gacagactga
    15121 acgccatcag tagcgtcacg ttcaagagca agaggtactc cacctgtttg gaaatcttca
    15181 aagatattcc agcttggttc gatggtatcc acacagacgt attccatcat attagcgaaa
    15241 ctttcattga gccaaaggtc atcccaccat ttcatagtca cgaggttccc aaaccattgg
    15301 tgagccaatt catgggccac aacaagggca acttgttgac ggctagcaaa tgtagagttc
    15361 tcatcgacaa ccaagtaaac ttcacggtag gtcacaagac cccagttttc catagcacca
    15421 gctgagaagt caggaagagc gatgtggaga gattgaggaa ttgggtactt aactccatag
    15481 taatcttcgt aaaactcgat agagcgaaca gcgatatcca gtgagaaatc aagatttgaa
    15541 agtggatgtg ctttggttga gtagacacct accagggtac catttttagt tttagcggtc
    15601 accccttgca aatcaccagc aacaaaggcc aacaagtaag aagacatgcg aggtgttgtc
    15661 tcaaacttcc agatacctgt ttccttacgg ttttcaacat cgatttctgg catgtttgac
    15721 aaggccaatt caccttctgc ttggtcaaag cgaagagaga ggtcaaaagt tgctttggct
    15781 tcaggctcat ccacacatgg gaaagcttcg cgcgcaaaat ggctctcgaa ctgagtagac
    15841 aagacctcct tcttgactcc atcaactgta taataagaag ggtaaatccc tgtcatgttg
    15901 tctgtaattt taccagaaaa ggcaagaacc aattcaactt gaccagcctc agccaattcg
    15961 atatgaaggg cttcattgtc atggtcaact gtaaatggac gagcttgacc tgcaacttct
    16021 acagaggtga tttccaagtc tttttggtgg agggagatgc ggtcactctg tgcttgacca
    16081 gtgatggtca ctttcccaga aaaagtcttg gtctcacgac tcaaatctaa aaataaatca
    16141 taatgttcag gaacaaattg cttaataaaa tgttcaactg cttgcatagt tttctcctat
    16201 tctaagttta agagctgctg ctcttcttca aacaaactta ttatatcata ttttgcttga
    16261 gaaaaaagga ttggacagcg atttccaaaa ctttcttcct tctaatagtc tacagtgggt
    16321 agaccttgcg aaattcttct aaaacaacct tgctttcagg tgtaaaagtc agccctactt
    16381 cacccaaaca gtctgtgaaa aagtcctcat gaacctggcg ccaataggca agtgatttgt
    16441 ctccctcacc ttccttaaag gcatgttgcg cagaaacttg attgaagagt tcaacagaaa
    16501 cctttgtaat ttcgacaatg cagacagact gattttgact atctaaaatg acatcgaagg
    16561 tccctacttg gggaagtggt tcgtcttcta gtacatagag gtcataggct gatgctgttg
    16621 ctgtcttttc tcctttaaac accaaatccg ctaaaaggtc tggttcaact ccaaaagccc
    16681 aagcatcgat ttcatctccg atcaaaggat tgatttgttt gtatttattc cacatttctt
    16741 gcggtgtcat gggtgctcct ttgtaatttt ttactttctt cttttatgtg tttaagatga
    16801 tctggatggt caatctctaa atcaaaaatc tctggaatag aactgtagtg gataatgcac
    16861 ttgataccca actgattcat tttttgtatg aaagaagtat tcagatagcc tgctacagca
    16921 aaattaatct tgttctttct tgctttatcc tgcatatctc ttagcatatc taacattatt
    16981 ggactttcca tatcatgcca ttgactgttt ctcatagtcg caaaaacaaa ggaagtcaaa
    17041 tcattcattc caactacaat ctttgaaatg cccgtttcca gtatactaga taagtcaaaa
    17101 tacgctgacg gtaattcaat catcgttccg actttcccag taaagccata ctgacgcaat
    17161 actgtaatag cttgttttaa ttggtcggca tcattgacaa aaggaaaaat aagagacaga
    17221 ttgggattgg tttgataaac ttctgtaacg acatgtgctt cagcctgaaa ttcatccaaa
    17281 cacgccagta aacgcctggt tcctctatag ccaaacaagg gatgcccttc gtcaaaaaac
    17341 tctctagtcc ccgctaaaca attggcttct gtattcgtta attcagaaaa acgataccaa
    17401 acttccttat ctaagtaaaa ggagcaaata gtatcaagat aatcttttac aaattgctga
    17461 caacttgcta atatgatgtt ttgattaagc tctctcaaca aatattcccc acgaatcatg
    17521 ccgacgtggt gaaatagttg aggataaatt ttttcaagaa ttttttcgcc actaagggca
    17581 agttgatttc tcatcattca ccttccaatt catgtaagaa gtcttgtcca gttctggaaa
    17641 tcctaataat tcagacttaa ccttcaagac taatggcgat gcattttctt ctgtaatctc
    17701 ttgaatatcc atccaaatat atccaagtga atcattcgca ccatcagaca cagcttccga
    17761 agtcgtaact tgaggtgcac tctcattcat ttcaacatca tacaaggcca tgacatggtg
    17821 aaccataaaa tttgttaact cttctctgac gaaaacatcg tagattcgag gattagagta
    17881 gcttctaaca gtaaatcccg tctcttccat aacttctcta gtcagcgttt ccgtcagtcc
    17941 ttcaccaagt tgctgactgc ctccaggtag atcataccga tgttgataag ggcctctcgt
    18001 tttttcaatg cagagtaact tcccattttc taagcaaaca gcgtagactc caaagtgttt
    18061 tttgatttcc atccaactcc tcctacttca aagaccagcc accatctatt gtcaagattt
    18121 gtccttgcat ggcgctcgct tttccacttg ctaaaaaaag actaagctct gctatttcct
    18181 ctggctcaat ccagcgcttg attggggttt cactagccac ccagtcagcc aaaccacctg
    18241 gttcaaaatc cgcagcggtc atagctgtct tgactgctcc tggagcgata ccaaagacct
    18301 gaatcccagc ttcagcatag tctagagcca actgcttggt gaagccagcc aaggcatgct
    18361 tggatgaagt ataggcgtga ccacctccac ctgctaggct agaagcaatg gaacacatat
    18421 tgatgatgat tcccttttta ttttccagca tttgtgtcaa ataataccga gtcaactcta
    18481 ctggaataat gtagttgatt tcaaaaatct cttgaatgtc ctgcgccgtt tgttccaaca
    18541 gtggtttgta atcatccaaa actccagcag tattacacaa aacatccacc tgagggcacc
    18601 agtcaaaaat aggttccaag tccaaggtca aatctctctg taaaaagtga aaatcaccct
    18661 ctaagagtgg cttttcacct tggtcaactc cataaacttg atagcccttc tctaaaaaga
    18721 ggcgagcttg agccaatccg atccctgaac tcactcctgt aatgagtaca cgtttagtca
    18781 tgcacttcta cccaatccgt tgccaaaaca tcacaaactg tcgggctcca catggaaaaa
    18841 ccttctcctt cgccagaaac gttgattagg aaataaggtg tcatttcaag tgcaagccca
    18901 ttttgctcga tggtatcaaa gagttggaca tagttttccg cacctcccca accagttcgt
    18961 acatattttc tcttagcctt taacccaggc aggatctctt caaatgtcat gtttttctcc
    19021 tttaattcta cattcttcat ttaattatag caaaaaaccg ctttatacgg ctttttgaat
    19081 gtgagttatt caaacctgct actacttacg gcaaattatt ccctgcagca agataaattt
    19141 cataccattc ttttcttgtt aagctaaagt ttgccgctcg gctaacttct ctcaagtgct
    19201 taggatttgt tgtacctacg actgcctgca tttttgctgg ataacgcaat atccaagaaa
    19261 tggcaatagt tgaagaggtt actccatatt taatagctaa acgatcaagt acttgattta
    19321 aagcttgaaa tttctcattt ccaacaaaat ttcctttaaa atacccgaat tgtaagacag
    19381 accatgcttg aatgaccaca tcgtgtaatt ggcaatattc aaaaatgctg ccatctcgca
    19441 tagctgcttg actatcttcc atattaacat gaaaagctga ttcaaatcct ggagtaaaag
    19501 ccgcactcaa ttgtagctga ttaacagcta acggctgctt gacatctttt ttaagcaact
    19561 ccatcatcat aggattttga ttagaaactc caaaatctcg aactttacct tgtttataaa
    19621 ggagatcaaa ggcttctgct acttggtcag attccatcaa agcatctggt cgatgaagga
    19681 gcaagctatc tagatgatca atcttcaatc tttgcaaaat accgtctact gattttataa
    19741 tatagtcctt agaaaaatca aaataggtaa attcttcaat gcgaatgcca catttggact
    19801 gaatccacat cttttctctt aaatctggac gattttttag gacaagacct aacagttctt
    19861 cacaacgacc acgaccataa atatcagcca agtcgaaggc attgattcca acagaaagtg
    19921 ctgtttctac aagctcttca acttctttta cagatttatc ttttattctc atcattccga
    19981 gaacaatttc tgataattct ttgtcatctt gaccaagagt tatgtatctc atcaaatttt
    20041 tctcctttaa tttctaacat tcttcccttc attataacaa aaaaccgctt tgcaacgact
    20101 ttttgactat acttcactcc attttatctt cttaaaccca cggaacaaga caaagattcc
    20161 aataaagagg acagctaaag gaataacttt tgtaaggaaa acatttgaaa ttcccatcca
    20221 ctcataataa cggagcagag aacccaccac aagatgggca ataatcatac tgacaaatgg
    20281 acgaaagacc gcttctttcc aattccaaat accgataact agcgaaatcg taaagataga
    20341 caaactatcc cagggagccg gaatataaaa ggctccttct tgtatgaagc ttgccattcc
    20401 tacatatcct aaaacaacta gaagaactat agtcccaaca acaatgtaag tgccaatttt
    20461 cattttagga gaatcttgga ctaaacttct tcgtaaaatt gtggccacaa gtccaaatcc
    20521 aatcagaaaa ataagaagtt gccctaaaaa tgtgagcaaa ttgactgtta agagaggacc
    20581 tttagaaaaa tcacttagta gttgataata acgtaatacc gccaggacaa gaattggcgt
    20641 caaaagggac tctttgatag aactgcgagg tgctcccttg agaatctctt tcattatttt
    20701 tttaggattc ttacctagat aatcctctgc actcatgcca tctcgttctg cttctgagaa
    20761 atctagcatc atcaaataga tctgctctct gagatagtct tcatcataga gaaatccagc
    20821 aagattaaaa ctttcccaca actcctcaaa atacttttga ttctcctcag aaaactcatg
    20881 tagcaaagcg cttgtttctt cgtaatactt cattttcttc atggtttaac ccccattctt
    20941 aatcccttct actttttgac tcaaatcgtc ccattgttgc caaaagactg agacacgctc
    21001 ttctccttct ttcattaatg aaaaatactt ccgatctgga ccatctggcg acgggcgcat
    21061 gtcgcctctt atccattgat ttttttctaa cttttgcaac aaaggataaa tagttcctgg
    21121 aacgatagta tcaaatccag cctctcgcaa agtctgaacc aactcataac cataccgctc
    21181 tttttgacca atcatatcca agacacaacc ttcaagaaca ccttttaata gctgagtttc
    21241 tttcatcact tctcccttct aatctatttt gtaataccta ctagtaactt cacctatagt
    21301 atattacttc tacactagtt tgtaaagcat aatagttaat actcttcgaa aatctcttca
    21361 aaccacgtca gcgtcgccct accgtatgta tggtt
//
LOCUS       DAVHIT010000031        20711 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVHIT010000031 DAVHIT010000000
VERSION     DAVHIT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.031"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            149..463
                     /locus_tag="VVN16_001004"
     CDS             149..463
                     /locus_tag="VVN16_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5572415.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            479..865
                     /locus_tag="VVN16_001005"
     CDS             479..865
                     /locus_tag="VVN16_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5572416.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            894..2279
                     /locus_tag="VVN16_001006"
     CDS             894..2279
                     /locus_tag="VVN16_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5572417.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2282..2434
                     /locus_tag="VVN16_001007"
     CDS             2282..2434
                     /locus_tag="VVN16_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5572418.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2457..3661
                     /locus_tag="VVN16_001008"
                     /pseudo
     CDS             2457..3661
                     /locus_tag="VVN16_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            3704..3925
                     /locus_tag="VVN16_001009"
     CDS             3704..3925
                     /locus_tag="VVN16_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572419.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4042..4539
                     /locus_tag="VVN16_001010"
     CDS             4042..4539
                     /locus_tag="VVN16_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU5572420.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4514..5020
                     /locus_tag="VVN16_001011"
     CDS             4514..5020
                     /locus_tag="VVN16_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5572421.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5004..7451
                     /locus_tag="VVN16_001012"
     CDS             5004..7451
                     /locus_tag="VVN16_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5572422.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7454..9631
                     /locus_tag="VVN16_001013"
     CDS             7454..9631
                     /locus_tag="VVN16_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5572423.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDRVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9628..10629
                     /locus_tag="VVN16_001014"
     CDS             9628..10629
                     /locus_tag="VVN16_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU5572424.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10626..11558
                     /locus_tag="VVN16_001015"
     CDS             10626..11558
                     /locus_tag="VVN16_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5572425.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            11810..13729
                     /gene="tet(M)"
                     /locus_tag="VVN16_001016"
     CDS             11810..13729
                     /gene="tet(M)"
                     /locus_tag="VVN16_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU5572426.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEILDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            13848..14015
                     /locus_tag="VVN16_001017"
     CDS             13848..14015
                     /locus_tag="VVN16_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU5572427.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14075..14428)
                     /locus_tag="VVN16_001018"
     CDS             complement(14075..14428)
                     /locus_tag="VVN16_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5572428.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            14933..15355
                     /locus_tag="VVN16_001019"
     CDS             14933..15355
                     /locus_tag="VVN16_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5572429.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15352..15582
                     /locus_tag="VVN16_001020"
     CDS             15352..15582
                     /locus_tag="VVN16_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5572430.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16043..16246
                     /locus_tag="VVN16_001021"
     CDS             16043..16246
                     /locus_tag="VVN16_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU5572431.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            16328..17545
                     /locus_tag="VVN16_001022"
     CDS             16328..17545
                     /locus_tag="VVN16_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5572432.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(17722..18309)
                     /locus_tag="VVN16_001023"
     CDS             complement(17722..18309)
                     /locus_tag="VVN16_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5572433.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKLLCIGELTILQILYCASLIS
                     FLWMYGQRKQVVKVNMKTRMKWLGIGFVSLLIISLCFSLIHAQGTTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGIFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            complement(18312..18545)
                     /locus_tag="VVN16_001024"
     CDS             complement(18312..18545)
                     /locus_tag="VVN16_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5572434.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            complement(18558..18938)
                     /locus_tag="VVN16_001025"
     CDS             complement(18558..18938)
                     /locus_tag="VVN16_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU5572435.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQKYGIEVSCHSISEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELCIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSS"
     gene            complement(18931..19380)
                     /locus_tag="VVN16_001026"
     CDS             complement(18931..19380)
                     /locus_tag="VVN16_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572436.1"
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQLFLMPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDNQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKSINFSNSLDSLIVSEYQMEPNLWEVSHD"
     gene            complement(19364..>20711)
                     /gene="dcm"
                     /locus_tag="VVN16_001027"
     CDS             complement(19364..>20711)
                     /gene="dcm"
                     /locus_tag="VVN16_001027"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU5572437.1"
                     /translation="LDLFAGIGGFRMGMESVGHECIGFCEIDKFARESYKSIFQTEGE
                     IEFHDIRNVSDDEFKKLRGKVDIICGGFPCQAFSIAGRRLGFEDTRGTLFFEIARAAK
                     QIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRERVF
                     IIGHSRKKGTRLLFPFRREGQATNSEILKTLGNLNPSKSGMSGKVYYSEGLAPTLVRG
                     KGEGFKIAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFKETG
                     RVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPS
                     SQYRRGRVGKGIANTLTTSGQMGVVGSYEGEDKQVYHVAGVLIDGQFYRLRIRRITPK
                     ECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDESIK"
BASE COUNT         6713 a         3824 c         4164 g         6010 t
ORIGIN      
        1 caaaaaatga ggtgtctatt tttttacccg attttgaaag gaagtgaact tatgaaaaca
       61 aaaaatcaag aatcaaaagg tcgttcccca ctctttaaga ccatcaaaca ttcattcagc
      121 caataaaaaa gaaaggatag gtaaaaatat ggaacttaaa tttgtgattc ccaacatgga
      181 aaaaacattc ggcaatttag aatttgctgg cgaggataaa gtcgttcagc gaagaatcaa
      241 cggacggcta actgtcttat caagaagcta taatctctat tctgatgttc aaagagcaga
      301 tgatattgtg gtggtgcttc ctgctgaagc tggcgaaaaa catttcggct ttgaggaacg
      361 tgtgaagtta gtcaatccac gtattaccgc agagggctac aaaatcggca ctcgtggttt
      421 tacaaattac cttttacatg ctgacgacat gataaaagaa taaagaaaga gaggaaaaat
      481 gatgagatta gcaaatggca ttgtattaga taaagacacg acttttggag aattgaaatt
      541 ctctgctcta cgtcgtgaag tgagaatcca aaatgaagac gggtcggttt cagatgaaat
      601 caaggaacgt acctatgact taaaatccaa aggacaagga cgcatgattc aagtaagtat
      661 tcctgccagc gtgcctttga aagagtttga ttataacgca cgggtggaac ttatcaatcc
      721 cattgcggac accgttgcta ctgccaccta tcaaggagca gatgttgact ggtatatcaa
      781 ggcagacgat attgtgctga caaaggattc tagttcattc aaagctcaac cacaagcaaa
      841 gaaagaaccg acacaagaca aatagtcgct aggtagaaag gagacttttt cgcatgaaac
      901 agcgtggtaa aaggattcgc ccatctggta aagatttagt ctttcatttt acgatagcgt
      961 cactcctgcc tgttttcctg ctggttgtcg gactgtttca tgtgaagaca atccagcaga
     1021 tcaactggca ggattttaac ctatcacaag cagataagat tgacattccc tatttaatta
     1081 tcagtttcag tgtcgcaatt cttatctgct tgctggtagc gtttgtattc aaacgggttc
     1141 gctatgatac ggttaaacaa ctttaccacc gtcaaaaact ggcaaagatg atacttgaaa
     1201 acaagtggta tgaatctgaa caggtcaaaa cagagggttt ctttaaagat agtgctggtc
     1261 gtacaaagga aaagataacc tacttcccta aaatgtatta tcgacttaaa aatggcttga
     1321 tacagatacg ggtggaaatc acgctgggaa aatatcaaga ccaactctta cacttggaaa
     1381 agaaattaga gagtggcttg tactgtgagc tgacggataa agagttaaag gattcctatg
     1441 tggaatatac tttgctctat gacaccatag ccagtcgtat ttctattgat gaagtagaag
     1501 ctaaagatgg taaacttcgc ttaatgaaaa acgtatggtg ggaatatgat aagctccctc
     1561 atatgttgat tgctggtggt acaggtggcg gtaaaactta ctttatactg acactgattg
     1621 aagccttgct tcatacagat tcaaaactgt atattcttga cccgaaaaat gctgatcttg
     1681 cggacttagg ttctgtgatg gcaaatgtct actatagaaa agaagacttg ctttcttgca
     1741 ttgaaacatt ctatgaagaa atgatgaaac gtagtgagga aatgaagcag atgaagaact
     1801 ataagactgg caaaaattat gcttacttag gtctcccggc acacttctta atctttgatg
     1861 aatacgtcgc tttcatggaa atgctgggaa caaaagaaaa caccgcagtt atgaataagc
     1921 tgaaacagat tgtcatgtta ggtcgtcaag ctggcttctt tctaatactg gcttgtcaac
     1981 gtccagacgc aaaatattta ggcgacggaa tccgtgatca gtttaatttc agagtggctt
     2041 taggtcgtat gtctgaaatg ggctatggca tgatgtttgg cagtgacgta caaaaggatt
     2101 tcttcttaaa gcgaatcaaa ggtcgtggct atgttgatgt aggaacaagt gtcatatcag
     2161 agttttatac tccccttgta ccaaaaggat atgatttctt ggaggaaatt aaaaagttat
     2221 ccaacagcag acagtccacg caggcgacgt gcgaagcgga agtcgcaggt gtggactgat
     2281 cttgctggct ggtgtggcaa tagccacgcc agcacttaac cccccgtatc taacaggggg
     2341 gtacaaatcg acaggaaaca gtcaaaaaaa cattagaaaa tcctttggtt acaagggatt
     2401 tacaaaattt cagcgtatgt caaatgggct ttaaaagttg acatacgcct ttttgattgg
     2461 agggattttt actgaatgaa caaacttggt tacagcattt aaaagaaaaa cgcttggctt
     2521 atggactatc tcaaaaccgt ttagctgttg cgactggtat tacaaggcag tatctaagcg
     2581 atattgaaac aggaaaagtc aagccatcag aggatttaca gcagtccctt tgggaagctc
     2641 tggaacgctt caatcccgac gctccccttg aaatgctgtt tgattatgta agaattcgct
     2701 ttccgacaac agacgtacag caggtggtcg aaaacatctt acaactgaaa ctgtcctatt
     2761 ttcttcatga ggactatggt ttctattctt attcagagca ttatgcttta ggcgacatat
     2821 tcgtcctttg ctcccatgaa ctggacaaag gagttctggt ggaattgaaa ggtcgtgggt
     2881 gcagacaatt tgaaagctat cttctggcac aacaaagaag ctggtatgag ttctttatgg
     2941 acgttttggt ggctggcggt gtgatgaaac gccttgacct tgccattaac gataagacag
     3001 ggattttaaa tatccctgta ctcactgaaa agtgccaaca ggaagaatgt atctccgtct
     3061 tccgcagttt taaaagctat cgcagtggcg aactggtacg caaagaggaa aaggaatgta
     3121 tgggaaacac cctctatatc ggttcattac aaagtgaagt ttatttctgt atctatgaaa
     3181 aggactacga gcagtacaag aaaaatgata ttcccattga agacgcagaa gtaaaaaacc
     3241 gttttgagat tcgattgaaa aatgagcgtg ctattatgca gtccgtgatt tactcgtcta
     3301 tgacaatcca gagcataccg cctttaaaat tatcaatcgg tatatccgtt ttgtagataa
     3361 agacgattcc aaacctcgtt ctgattggaa actgaatgaa gaatgggctt ggtttattgg
     3421 gaacaatcgt gaacgattaa aactaaccac aaaaccagag ccttactcct tccaaaggac
     3481 gctgaactgg ctatctcatc aagttgcccc gaccttaaag gttgcgatta aacttgatga
     3541 aatcaaccag acgcaggttg taaaagacat tctcgaccat gcgaaactga cagaccgaca
     3601 caagcagatt ttgaagcaac agtcagtaaa agaacaggac gtgataacaa caaaaaaata
     3661 actcaaatac aaattcattg aatatagaga ggagaacatt tttatgaatt ttggacaaaa
     3721 cctttataac tggtttctat caaacgctca atcactggtg cttttagcaa tcgttgtgat
     3781 tggcttgtat cttggcttca agcgtgagtt tagcaaactg attggctttt taattattgc
     3841 gattattgcg gttggcttag tcttcaacgc tgctggagta aaagacattt tactagagct
     3901 attcaatcgc attattggtg cttaaataaa accgttcttt tgtggaatat aagtggtttt
     3961 cttatgttcc gcaaaggaat ggtacaccaa acgaagtgcg gtagggattt ttgaatctct
     4021 acaaagaaag gacgtgaata tatggacgat atgcaagtct atattgcgaa tttaggcaaa
     4081 tacaatgagg gcgaattggt cggtgcgtgg tttacctttc ccattgactt tgaggaagtc
     4141 aaagagaaaa tcggcttgaa tgatgaatat gaggaatacg ccattcatga ctacgagtta
     4201 ccctttacgg ttgacgaata cacttccatt ggcgaactca atcgactatg ggaaatggta
     4261 tcggaattac ccgaagaatt acaatcggag ctatctgctc tgctcactca tttttcaagc
     4321 attgaagaac taagcgaaca tcaagaggat attatcattc attccgattg tgatgatatg
     4381 tatgacgtgg cacgctacta cattgaagaa acgggtgctt taggcgaagt accagctagt
     4441 cttcaaaact atattgatta tcaagcctat ggtcgggatt tagacctttc aggaacgttt
     4501 atctcaacca atcatgggat ttttgaaatc gtctattaaa tctgtcggta cattactact
     4561 ggcagatttt ctattttacg gggtggctca atcagctacc cctatttttt atgaaaggat
     4621 tgattacatg aagaaaatac gaagctatac cagtatctgg tctgtggaaa aggtactgta
     4681 ttctatcaat gattttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt
     4741 cgtgtcactc tttgcagtga tgatacttgg caacttgccc cctctttcca tgatagaggg
     4801 agcatttctc aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac
     4861 ttttgatggt aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacc
     4921 aaagctgacc tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat
     4981 tacagcagtt aggagtgaat tttatggcat atccaattaa atacattgaa aacaatctcg
     5041 tctggaataa agacggggaa tgttatgctt actatgagct tgttccttac aattactcat
     5101 ttctaagtcc agaacagaaa atacaagtgc atgattcttt cagacagctt atcgcacaaa
     5161 atcgtgatgg caaaattcat gctttacaaa tcagtacaga atccagcata cgttctgcac
     5221 aagagcgttc caaaaatgaa gtcactggca agctcaaagc ggttgcctat gacaaaatcg
     5281 accaacagac agacgcttta atatccatga ttggcgaaaa tcaagtgaac taccgtttct
     5341 ttatcggctt taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg
     5401 aagcaaaaaa tgctttgtct gattttgtct atgatgtgaa ccataagctg atgggcgatt
     5461 ttgttagtat gagtaatgat gaaatcctgc gttttcagaa gatggaaaag ctcttagaaa
     5521 ataaaatctc tcgtcgtttc aaaatccgca ggttagataa ggacgacttc ggctatctga
     5581 ttgaacacct ttacggacag acaggcactg cctatgaaga gtatgagtac catctatcaa
     5641 agaaaaagct ggataatgaa acgctgatta aatactatga cttgattaag cctactcgct
     5701 gtttggtgga agaaaaacag cgatatttga aaatccagca ggaagatgaa accgtctatg
     5761 tagcttactt taccattaac agcattgtcg gagaactgga cttcccgtcc tctgaaatct
     5821 tctactacca gcaacagcaa tttacattcc cgattgatac gtcaatgaat gtggaaattg
     5881 tagcgaatcg taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg
     5941 ataaccacgc ttggcaaagt gataatgaaa ccagctccaa tgtggcggaa gctctggaaa
     6001 gtgtgaatga gctggaaacc aatttagacc aaagcaagga atctatgtac aagctgtctt
     6061 atgtggtaag ggtatcagca aatgatcttg acgaactcaa acgtcgttgt aatgaagtga
     6121 aagattttta tgacgattta agcgtaaaac tggtacgacc atttggggat atgctcggct
     6181 tacatgaaga atttttacct gccagcaagc gttatatgaa tgattatatt caatacgtga
     6241 cctctgattt cctcgctggt ttaggttttg gtgctactca aatgctgggg gaaaatgagg
     6301 ggatttatgt tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg
     6361 ccagtcaagg ggttaagggt tcagtaacca atgcgttagc gtcggctttt gttggttcgc
     6421 tgggtggtgg taaatccttt gcgaataacc ttatcgtcta ttatgcggtg ctttatgggg
     6481 cacaagcagt gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag
     6541 agatttccca tgaaatcaat atcgtcactc tgacttctga tgagaaaaac aaaggcttac
     6601 ttgaccctta tgtgattatg aaaaatccca aagattctga atcactggct attgatattc
     6661 tgacattcct tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag
     6721 ccattcgtgc agtaaccaat agtgaagtac gagggttgat gaaagtgatt gaggaattac
     6781 gggttgagaa tacgccacta agtaccagta tagccgacca tatcgaaagt tttacagact
     6841 atgactttgc acatttatta ttcagtaatg gttatgtgga gcagtctatc agcttagaaa
     6901 aacaactgaa cattatacag gttgcggact tggtacttcc cgacaaggaa acttcctttg
     6961 aggaatatac cactatggag cttttatccg ttgctatgct gattgtcatt agtacctttg
     7021 ctttagactt tatccataca gaccgaagca ttttcaagat tgtagattta gacgaagcat
     7081 ggagcttttt acaggtagca caaggaaaaa cactatctat gaagctggtt cgggctggtc
     7141 gtgctatgaa cgctggggta tatttcgtga cccaaaatac agacgacctc ttagatgaaa
     7201 aactgaaaaa taacctcggc ttaaaatttg catttcgttc cactgacctt aacgagatta
     7261 aaaagacctt agcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc
     7321 gtgatttgga aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac
     7381 agttccaccc tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa
     7441 aagaggtgta aatgtgaaac catcaatagt aaacagaata aaatcaaact ggacgctgaa
     7501 acgtctaggt aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt
     7561 agccatgctg ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc
     7621 aaatgaatac agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag
     7681 ctggggctgg cttccgtgga actggtcgga cgggattgga aaacaggtca tgtatggact
     7741 atatgccatt accaatttta tttggacaat cagtttgtat gtttccaatg cgacaggtta
     7801 cttagtacag gaagcctatt ccttagactt catttccgct acagcagatt ccattggtaa
     7861 gaatatgcag accttagctg gtgtgagtgc aaacggattt tcaacagagg gtttctatgt
     7921 tggattcctc ttactcttga ttttggttct tggggtttat gttgcctata cgggactgat
     7981 aaagagagaa accacaaagg caattcatgc cattatgaat tttgtgctgg tgtttatcct
     8041 atcggcttcc tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc
     8101 agacatcagt aatgccagtt tatcacttgg cacgaagatt gtcatgcccc attccgatag
     8161 tcaaggcaag gacagagtgg acttaatcag agatagcctg ttttccatac aggttcagca
     8221 accgtggcta ctgcttcaat acaacagttc agacattgaa agtatcggta ttgaccgtgt
     8281 ggaaagcctg ctctccacca gcccagattc caacaatggc gaagacagag aaaaaattgt
     8341 tgcggaagaa attgaagaca gaagcaatac caatctaacc attacaaaga ccattaaccg
     8401 tttaggtaca gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct
     8461 attaacagga atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc
     8521 tgtgagcttt attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac
     8581 aaagctcttt aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt
     8641 tagtatttca accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt
     8701 tctacagatt gtgacctttg caggaatcta cttcaagctg ggcgatttaa tgagtatgtt
     8761 ttctctacag agtaacgatt ctcaaagtgt gggaagtcgt gtgatgagaa aacctcgtat
     8821 gcttatgcac gctcacatgc accgtctaca gcggaaactt ggacgttcca tgactactct
     8881 aggggctggg tctgccattg ttacaggtaa aaaaggacag tcgggttcgg ggagttctgc
     8941 aaggacacaa gcagatcact cccgaccaga cggaaaggaa aaatcaacac ttggaaaacg
     9001 tatcggtcaa accatcggta cagtagctga taccaaagac agaatggtag acactgctag
     9061 tggtttgaaa gaacaggtta aagatttgcc gaccaatgca agatatgcag tatatcaagg
     9121 aaaatccaaa gtaaaagaga atgtccgtga tttaaccagt agtatttctc aaaccaaagc
     9181 ggacagagcc agtggacgca aggaacagca ggaacaaagg cgaaaaacca ttgcgaagcg
     9241 tcgctctgaa atggaacagg tcaaacagaa aaaacagcct gcttcttctg ttcatgaaag
     9301 accgactaca agacaagaac aatatcatga tgaacagacc tcaaaacagt ctaatattca
     9361 gacttcatat aaggaatctc aacaagccaa acaagagcgt ccagcagtta agtccgattt
     9421 ttcaagtcca aaagtggaac gccaaggcaa taccgttcaa gaaaaaaccg ttcaaaagcc
     9481 agcaacttca accactacag cagatagaac ttcacaacgt ccaatcacaa aagaacgtcc
     9541 gtctactgtt caaagagtac cactacaaaa tacaagaagt agaccaccaa tcaaaaccgc
     9601 caccattaag aaagtcggta agaaaccatg aagttgaaaa ctttagtgat tggtggttct
     9661 ggattattct tgatggtctt ctcactgctt ctgtttgttg ccattttatt ttcagatgaa
     9721 caggacagcg gaatttccaa tattcattat ggaggtgtga atgtttccgc agaagtgctg
     9781 gctcataagc ctatggtaga aaaatatgcc aaagaatatg gcgttgaaga atatgtcaac
     9841 atacttcttg cgattataca ggtggaatcg ggcggtactg cggaagatgt tatgcagtcc
     9901 tcggaatccc tcggtcttcc acctaattca ttgagtacag aagaatccat taagcaaggt
     9961 gtgaagtatt tcagtgaatt attagccagt agcgaaaggc tcagtgtaga tttagaatcg
    10021 gttatccagt cctacaatta tggtggtggt ttcttagggt atgtggctaa tcgtggaaat
    10081 aaatatacct ttgaactggc tcaaagtttc tcaaaagagt attcaggtgg cgaaaaagtg
    10141 tcttacccca atcccatagc catacctatc aatgggggct ggcgatacaa ctatggcaat
    10201 atgttttatg tgcaacttgt aacgcagtat cttgtcacaa cagagtttga tgatgatacg
    10261 gtacaagcca tcatggacga agcactgaaa tatgagggct ggcgatacgt ttacggtgga
    10321 gcttccccga ctacttcttt tgattgtagc ggactgacac aatggacgta tggaaaagct
    10381 ggaattaact taccacgaac cgcacaacag caatatgatg tgacccagca tatcccacta
    10441 tcggaagcac aagctggcga tttggttttc tttcattcta cctataacgc tggctcttat
    10501 attactcatg ttgggatata ccttggcaat aaccgtatgt ttcatgcagg cgacccaatc
    10561 ggttatgccg acttaacaag cccctactgg caacagcatt tagtgggagc aggacgaatc
    10621 aaacaatgag aaaggaagat ttaatgatga aatttagaaa aaatcagaat aaagaaaaac
    10681 agataccaaa ggaaaagaaa cctcgtgtct ataaggtcaa tcctcataaa aaggttgtga
    10741 ttgccttgtg ggtactttta gggcttagtt tcagctttgc gatattcaag cactttacag
    10801 ctatagatac tcatactatt cacgaaacaa ctatcataga aaaggaatac gttgatactc
    10861 atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg gagcaatccg
    10921 ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat gaacttcaag
    10981 ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta agaggatttc
    11041 agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac agtgtagacc
    11101 agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata gtgagtgtct
    11161 atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattact aacataccta
    11221 agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat tccattacaa
    11281 ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca gcgacagcca
    11341 gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa gagtacatct
    11401 ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg gtatcgctga
    11461 cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt gatttggtac
    11521 ttgaaaagaa cgggagtaat tggaagattg taaaataaca aatattggta catgattaca
    11581 gatactttgt aatcatgtac tctttttgat aaaaaattgg agattccttt acaaatatgc
    11641 tcttacgtgc tattatttaa gtgactattt aaaaggagtt aataaatatg cggcaaggta
    11701 ttcttaaata aactgtcaat ttgatagcgg gaacaaataa ttagatgtcc ttttttagga
    11761 gggcttagtt ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat
    11821 taatattgga gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt
    11881 atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga
    11941 taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca
    12001 gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga
    12061 agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg
    12121 cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat
    12181 cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat
    12241 taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat
    12301 gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga
    12361 ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga
    12421 aagcataaga tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa
    12481 tatagggatt gataacctta tagaagttat tactaataaa ttttattcat caacacatcg
    12541 aggtccgtct gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg
    12601 tcttgcatat atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc
    12661 agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa
    12721 gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa
    12781 tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc
    12841 tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc
    12901 ccttttggaa atcttagata gtgatccgct tctacgatat tacgtggatt ctacgacaca
    12961 tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca
    13021 agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc
    13081 gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc
    13141 cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt
    13201 ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg
    13261 ctgtgaacaa ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg
    13321 cttatactat agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt
    13381 ggaacaagtc ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat
    13441 ttatgcgcca caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa
    13501 catcgtagac actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg
    13561 gtgtattcaa gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt
    13621 aacagagtta aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc
    13681 aaatagtcgg atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg
    13741 ttgttatata aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga
    13801 attaaagtgt aaaggaggag atagttatta taaactacaa gtggatattg tgtcctgtat
    13861 gtggaaataa aacacgatta aagataaggg aagatactga attaaaaaaa ttccccctct
    13921 attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga
    13981 ttacagagcc agacgcaaag acgcagagcc gataaaatga gattaataca atctcatttt
    14041 atcggctctt tccgttatgt atggattctt ttaattagtc ttcgatgttt cttgcttcgt
    14101 tgataccgct ggctaaagat tccattaagg atagttcttt gtctgtaaag ctatccatgt
    14161 atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt
    14221 catcaacgga aacatgaagt aacgatacaa ggtcataaag aacttgtatg ctggggtgtt
    14281 gccctttatt ttcaatatta gttaagtacc gtgggtcaat ttcaatcaat gctcccactt
    14341 gttcacgagt taaacctcgt ttcaatcgag cttctttaat ggctaaacca aaggctctaa
    14401 aatcatattt atcttctttt ttacgcatag tagaccacct ctatacattt tattgttcct
    14461 actgaattaa aaacaggtat agaaaaacgt gttatatggt ttataggttt atatttaata
    14521 aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt
    14581 aaaatattgt atattacttc caaatggcgg tttgttggag gtcaacgtcg ccatgaagta
    14641 catcatatac aataaatttc cttacattgg gttcttgtca aaaaaagtcg tctatctgca
    14701 atagataagt acgtccacca atgtggtttt ataaatcata tagatagaat aacagaagca
    14761 tgtaaacaga gaaataaatc tgtttatatg cttttttggc tattcagaac ttttttacaa
    14821 agtttattta tcagtaatgc aacaaatccc cctttcacat tgggactaag agtgaaagga
    14881 gataaacgag caaggctcac ttcctttcct agacagaaag ggggtgagaa acatgaaacc
    14941 atcttctttt cagaccacaa tagaaaatca gtttgactat atctgtaaac gtgctatgga
    15001 agacgagcga aagaattata tgctttatct ttcaaggatt gcaaagcgtg aggtgtcctt
    15061 ttcggatgtt ggcgattatc ttgttagcca gtttgcgaca acagataact attcaactga
    15121 ctttcagatt tttacactca atgggttatc agtaggcgtt gaaaatgatt tgttgagtga
    15181 agcattacgt gagttgccag acaagaaacg tgaaattcta ctgctgtttt actttatgga
    15241 catgagcgat tcagaaattg cagacctgtt gaaattgaac cgttctactg tctatcggca
    15301 tagaaccagt ggactagcct taattaaaaa gtttatggag gaatttgaag aatgaaaaca
    15361 caatatccta tgattccctt tcctctcatt gtaaaggcaa cagatggcga taccgaagcg
    15421 attaaccaga ttctacatca ttacagaggg tacataacga agcgttccct acgacttatg
    15481 aaagatgaat atggcaatca aagtatggtc gttgatgaag tcttacgtgg aagaatggaa
    15541 accagactga ttacaaagat tttgtcattt gaaattaagt aatatcctct ctcctttcgt
    15601 ggaagcgtgc taaaccattc cacgcttccc gaacagggag gtttgttatt ccaccaaagc
    15661 atattgagct ttcaatgtgt tttgataggc taacgagcca ttgttctttg aaaactgaat
    15721 aaaagtaatc gaatacgttt cgataagaaa agagccaacg gaactaaccg ccatgaccta
    15781 tcttataaag atagcgagcg attcatgtta gtgatccgag aagcaatctt tagcaggatt
    15841 gcctgcaacg acattcttat cgtgataatg atactcccat acagtcaata gtccgagcgt
    15901 gataaaaccg tcgcaggcaa tgagtatggc tacatgagaa ccatgcaggg gtggaactcc
    15961 cgtgagcttt gctaaagctg ttcgattgct ggtaaaacaa cttttatgaa atccaaataa
    16021 gtgatttgga aaggaggatt ttatgaagca gactgacatt cctatttggg aacgttatac
    16081 cctaaccatt gaagaagcgt caaaatattt tcgtattggc gaaaacaagc tacgacgctt
    16141 ggcagaggaa aataaaaatg caaattggct gattatgaat ggcaatcgta ttcagattaa
    16201 acgaaaacaa tttgaaaaaa ttatagatac attggacgca atctagcgtc gccaaagggt
    16261 cttgtatatg ataaaatagt attaagtcgt atcaaggctc tttccataaa ggaaaggagc
    16321 aaatgccatg tcagaaaaaa gacgtgacaa taaaggtcga atcttaaaga ctggagagag
    16381 ccaacgaaaa gacggaagat acttatacaa atatatagat tcatttggag aaccgcaatt
    16441 tgtttactcg tggaaacttg tggctacaga ccgagtacca gcaggaaagc gtgattgtat
    16501 ctcacttaga gagaaaatcg cagagttaca gaaagacatt catgatggta ttgatgttgt
    16561 aggaaagaaa atgacactct gccagcttta cgcaaaacag aacgctcaaa gaccaaaggt
    16621 tagaaaaaac actgaaactg gacgcaaata tcttatggat attttgaaga aagacaagtt
    16681 aggtgtaaga agtattgaca gtattaagcc atcagacgct aaagaatggg ctattagaat
    16741 gagtgaaaat ggttatgctt atcaaaccat caataactac aaacgttctt taaaggcttc
    16801 attctatatt gctatacaag atgattgtgt tcggaagaat ccatttgact ttcaactgaa
    16861 agcagttctt gatgatgata ctgtccctaa gaccgtacta acagaagaac aggaagaaaa
    16921 actgttagcc tttgcaaaag ctgataaaac ctacagcaaa aattatgatg aaattctgat
    16981 actcttaaaa acaggtcttc gtatttcaga gtttggtggt ttgacacttc cagatttaga
    17041 ttttgagaat cgtcttgtca atatagacca tcagctattg agagatactg aaattgggta
    17101 ctacattgaa acaccaaaga ccaaaagtgg cgaacgtcaa gttcctatgg ttgaagaagc
    17161 ctatcaagca tttaagcgag tgttagcgaa tcgaaagaat gataagcgtg ttgagattga
    17221 tggatatagt gatttcctct ttcttaatag aaagaactat ccaaaagtgg caagtgatta
    17281 caacggcatg atgaaaggtc ttgttaagaa atacaataag tataacgagg ataaattgcc
    17341 acacatcact ccacatagtt tgcgacatac attctgtacc aactatgcaa atgcaggaat
    17401 gaatccaaag gcattacagt acattatggg acatgctaat atagccatga cgctgaacta
    17461 ttacgcacat gcaacattcg attctgcaat ggcagaaatg aaacgcttga ataaagagaa
    17521 gcaacaggag cgtcttgttg cttagtagta caaatgaatt tactacttat ttaccacttc
    17581 tgacagctaa gacatgagga aatatgcaaa gaaacgtgaa gtatcttcct acagtaaaaa
    17641 tactcgaaag cacatagaat aaggctttac gagcatttaa gaaaatataa aaagataatt
    17701 agaaatttat actttgtttg tctaaaaaag cagcaaacta taaactagta ggttccacac
    17761 caaatgtaga cccatacttc cccataagtc cgatttatag cgcaccatcc ctaaaaagat
    17821 cccaagtgaa acatataaac accaagctag aatggttcct ggatgatgta ctaaggcaaa
    17881 taaaacactt gtcaaagcaa ctcgaatatc taattttctg accaggttcc ataaaatttc
    17941 tcgatacaga aattcttcaa ccatactcgc attgattaag aacaataaaa atgaaaacca
    18001 aggaacctga tgttgaagtc caattaagtt tgcttgattc gtggttcctt gagcatgaat
    18061 cagactaaaa cataggctta taatcagtag gctaacgaat ccaataccaa gccatttcat
    18121 cctagttttc atattgacct tgaccacttg ttttcgttgg ccatacatcc ataaaaaaga
    18181 aatgagcgac gcacaataga gaatctgtag tatggttaac tcaccaatac aaagcaattt
    18241 cagtaagtat agggatacca ataagacatt tacttgttgg aatatataaa ctggaattat
    18301 tcttttcata gttacctccg aaataaatct tcataatcta aatctaatac ctgcacaatc
    18361 ctttctaccc atggactttg aggcattcgt tgttccatct tgtagtggcg tattttttga
    18421 tacaaacgat tcaattcact tggatagtga aactctcccg caaacatttt tctggttaac
    18481 tcaatccagc taatatttct ttcagccaaa ataatggaca agttctccca aaatcgttca
    18541 gccatattgc ttctccttta actagataaa taatgtgttt gtgccatgta aatcaattgt
    18601 ttcgtatctc ttggcaatag agctctagcc tcttccaaat tcagatttgg ataaactctc
    18661 ttatttgaaa ccgcaagagg aagcctgatg catagttcag gattttttaa tatcatttca
    18721 atgaaatccg ttaatcttag attgtcacgg ttcttaaatc gtaataaatt gggagataga
    18781 aactcaaaac aatctgaaga atagctcatc atctcaatta atttatcctt tgtcatttca
    18841 gaaatcgaat gacaagacac ctcgatgcca tacttttgaa agaaatccaa aagaagttga
    18901 tttctttggc tatttttact tagatagaga tcaatcatgg gagacctccc aaagattcgg
    18961 ttccatttga tattctgaga cgattaagga atctaatgaa tttgagaagt taatcgattt
    19021 cttgtcttca tcataagctt ttacagttac ttgggttgta agtattcctt cttttccctc
    19081 ggctcgatag ccttgtccat ataaaacaaa aacaagattc tgattatcat ctacaaaggc
    19141 atcaacccca ttctttatgt cttgactttc aaggaattcc ataacgtttt gaagatagga
    19201 ctcgtaaaat agtgggtagt tgtgtttttt atggtaatca tctaaaaatg ttacttcaaa
    19261 ctcacatgga taattgggca tcaaaaataa ttgttcatcc agctgtttga tttctgcatc
    19321 atgtaattct gtttctaatt catcacaatc tagtattgat tctttattta atgctttcat
    19381 ctttttcctc tatttctttt aatttctttg cgatcgcggc aatcacagga acggttacac
    19441 tattaccagc ttgtttatag agctgactat tactagagac ttttctcgca gcttcaaaag
    19501 cccaatcagg aaagccctgc aagcgaaaac actctttagg agtgattcgt cgtatcctca
    19561 gacggtaaaa ttgtccatct atcaagacac cagctacatg ataaacttgt ttatcttctc
    19621 cttcatagct acccactact cccatttgac cactagttgt taacgtattc gctatacctt
    19681 ttccaactct accacgccga tactgagaac ttggtctttc caaattgatt gaatctccaa
    19741 tctctgcttg agcgtatcct tttttagttg cttccctgac ttttaaaaat tggattggtt
    19801 ctggaatcag tattttggga attttatctc ctccttgcat cgtagtcagt gttggagata
    19861 agccctcact tccatagacg cgaccggtct ccttaaagct agtgggtaaa tctccaacaa
    19921 cgacaatgcc atgacgatcc tgagtattta aagtaaacat cggctcttga ttttccttaa
    19981 agcgtctacc attttgtctc ttgtctaatc tgtctggtgt catacaagga atcgcaatct
    20041 taaatccttc tcctttccca cgaactaagg ttggcgcaag accttctgaa taataaactt
    20101 taccgctcat tccacttttt gatggattca aatttcctaa tgtttttaaa atctcagagt
    20161 tagttgcttg accttctcgt ctgaaaggaa ataagagtct ggtacctttc tttctagaat
    20221 gtccgataat aaacaccctc tctctgtttt ggggaacgcc aaaatcctta ctgttaagca
    20281 cctgccactc aacatcaaac cccaactcat caagtgtggt aaggattgtg gtgaacgtcc
    20341 gtcccttatc gtgattgagt aggcctttaa cattttcaag aaaaagaaaa cgtggttgga
    20401 tttgtttggc cgcccgagca atttcaaaga acaaagttcc tctagtatct tcaaatccca
    20461 atcgtcttcc tgcgattgaa aatgcttgac aagggaatcc cccacagatg atatcgactt
    20521 tccctctaag ttttttaaat tcatcatctg aaacatttcg tatgtcatga aattctattt
    20581 ctccttccgt ttgaaaaatg gatttataag attctctagc aaatttatca atctcacaaa
    20641 atccaataca ctcgtgtccg acactttcca ttcccatcct aaagcctcct atcccagcaa
    20701 ataaatctaa a
//
LOCUS       DAVHIT010000032        20038 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVHIT010000032 DAVHIT010000000
VERSION     DAVHIT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.032"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            78..3179
                     /locus_tag="VVN16_001028"
     CDS             78..3179
                     /locus_tag="VVN16_001028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5572438.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRVSFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3262..4269
                     /gene="pfkA"
                     /locus_tag="VVN16_001029"
     CDS             3262..4269
                     /gene="pfkA"
                     /locus_tag="VVN16_001029"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU5572439.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEDGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4328..5833
                     /gene="pyk"
                     /locus_tag="VVN16_001030"
     CDS             4328..5833
                     /gene="pyk"
                     /locus_tag="VVN16_001030"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5572440.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAIEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6413..6638)
                     /locus_tag="VVN16_001031"
                     /pseudo
     CDS             complement(6413..6638)
                     /locus_tag="VVN16_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7486..8358
                     /locus_tag="VVN16_001032"
     CDS             7486..8358
                     /locus_tag="VVN16_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU5572441.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8504..8848)
                     /locus_tag="VVN16_001033"
                     /pseudo
     CDS             complement(8504..8848)
                     /locus_tag="VVN16_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            8991..9303
                     /locus_tag="VVN16_001034"
                     /pseudo
     CDS             8991..9303
                     /locus_tag="VVN16_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9307..10023
                     /locus_tag="VVN16_001035"
     CDS             9307..10023
                     /locus_tag="VVN16_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5572442.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10153..10968)
                     /locus_tag="VVN16_001036"
     CDS             complement(10153..10968)
                     /locus_tag="VVN16_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU5572443.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNNRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(10965..11870)
                     /locus_tag="VVN16_001037"
     CDS             complement(10965..11870)
                     /locus_tag="VVN16_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU5572444.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            12282..14411
                     /locus_tag="VVN16_001038"
     CDS             12282..14411
                     /locus_tag="VVN16_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU5572445.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14398..14847
                     /locus_tag="VVN16_001039"
     CDS             14398..14847
                     /locus_tag="VVN16_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU5572446.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            14906..15178
                     /locus_tag="VVN16_001040"
     CDS             14906..15178
                     /locus_tag="VVN16_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5572447.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15532..15723)
                     /locus_tag="VVN16_001041"
     CDS             complement(15532..15723)
                     /locus_tag="VVN16_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572448.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16151..16909
                     /locus_tag="VVN16_001042"
     CDS             16151..16909
                     /locus_tag="VVN16_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572449.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            16911..18899
                     /locus_tag="VVN16_001043"
     CDS             16911..18899
                     /locus_tag="VVN16_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572450.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(18942..19520)
                     /locus_tag="VVN16_001044"
     CDS             complement(18942..19520)
                     /locus_tag="VVN16_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5572451.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGGEYVSVSTPKDTEEAA
                     VSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLLTNKIFLKNPLKALVEEKYGIEY
                     EEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRIPATVLIVGVALLLTGYTSARL
                     GKAPTKTAMIRNLAIGLLTMGVTFLLGQLFSI"
     gene            complement(19516..20038)
                     /locus_tag="VVN16_001045"
                     /pseudo
     CDS             complement(19516..20038)
                     /locus_tag="VVN16_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         6126 a         3632 c         4244 g         6036 t
ORIGIN      
        1 tgaaattttt ctcaagagaa gccatttata ggtgaaaatg gtataatata gtgagaagga
       61 tagaggagaa gtgtaaattg atcgcacaac tagatacaaa aacagtctat agttttatgg
      121 aaagcgtcat ttcgatcgaa aagtatgtga gagcagctaa agaatacggc tacactcatt
      181 tggctatgat ggatattgac aatctttatg gcgctttcga ctttctagag attacaaaaa
      241 aatacggcat tcatcctttg ctagggcttg aaatgacagt gtttgtagat gatcaggaag
      301 tgaatttgcg ctttttagct ctatctagtg tgggctatca gcagttgatg aagctttcga
      361 cagccaagat gcagggggag aaaacttggt cagtcctgtc ccagtacctg gaggatatcg
      421 cggtcattgt gccttatttt gatagagttg agtcgttaga actaggctgt gattactata
      481 taggggttta tccagaaaca ctagcaagcg aatttcatca tcctatctta cctctttatc
      541 gggtcaacgc ttttgaaagc agggatagag aagttcttca agttttaaca gcgattaaag
      601 aaaatctacc gctcagagaa gttcccttgc gttcgagaca agatgtcttt atatcagcaa
      661 gttctttaga gaaactattc caagagcgtt ttccgcaagc tttggacaat ttagaaaagc
      721 ttatttcagg catttcttac gacttggata ctagtctgaa actgcctcgt tttaatccag
      781 ctagaccagc agtagaggag ttgagagagc gtgctgaact ggggcttgtt cagaaggggt
      841 tgactagtaa agaatatcaa gatagactag accaagaatt gtctgttatt catgatatgg
      901 gctttgatga ttatttcttg gttgtttggg atttgttgcg ttttggacga tcgaatggct
      961 attatatggg aatgggaagg ggttctgcag taggcagttt ggtttcttat gccttagaca
     1021 tcacggggat tgacccagta gagaaaaatc tgatttttga acgctttctt aatcgtgaac
     1081 gctataccat gcctgatatt gatattgata tcccagatat ttatcgtcca gattttatca
     1141 gatatgttgg taataaatat ggtagtaaac atgcggcaca aatcgttact ttttcaacct
     1201 ttggagccaa gcaagctctt cgagatgtct tgaaacgctt tggtgtgcca gagtatgaat
     1261 tatctgcaat tactaagaaa atcagttttc gtgacaatct taagtcggcc tatgagggca
     1321 atctccagtt tcgtcagcaa atcaatagta agttagaata ccaaaaagct tttgagattg
     1381 cttgcaagat agagggctat ccaagacaaa cctctgtcca tgcggctggt gttgtaatta
     1441 gtgaccaaga tttaaccaac tatattcctc taaagtatgg tgatgaaatt ccactgactc
     1501 agtatgatgc tcatggagtt gaggctagcg gacttttgaa gatggacttt ctgggactac
     1561 gaaatttgac ctttgtccag aagatgcaag agttgcttgc tgaaatagaa ggtattcacc
     1621 ttaaaattga agaaatagat ttggaagaca aagaaacgtt agatttattt gcctctggaa
     1681 atacaaaagg tatctttcaa tttgagcaac caggtgccat tcgtctgctt aagcgtgtgc
     1741 aaccagtctg ttttgaagat gtcgtcgcga ctacttctct aaatcgaccg ggtgctagtg
     1801 actatatcaa taattttgtg gcaagaaagc atgggcagga agaagtgact gttctggatc
     1861 cagtactgga ggatattttg gctccaacct acggcataat gctctatcag gagcaggtta
     1921 tgcaggttgc ccagcgattt gccggattta gtcttgggaa agccgatatt ttgcgtcggg
     1981 ctatggggaa aaaggatgcc tctgccatgc atgagatgag ggtttccttt attcaaggtt
     2041 cattagaagc tggtcatact gtggaaaaag cagagcaggt ctttgatgtt atggagaagt
     2101 ttgcaggtta tggttttaac aggtcacacg cctatgccta ctcagccttg gccttccagt
     2161 tggcttattt taaaacacat tatccagcca ttttttatca ggtcatgtta aattcttcca
     2221 acagtgatta cttaatagat gcacttgaag caggttttga agtagcctct ctatccatca
     2281 acaccattcc ctatcacgat aaaattgcca acaaggccat ctatctaggt ttgaaatcca
     2341 ttaaaggagt cagtaatgat ttagctctct ggattattga aaatagacct tattctaaca
     2401 ttgaagattt tatagctaaa ttacctgaga attatctgaa acttcctctg ctagaacctt
     2461 tggtaaaagt tggtcttttc gattcatttg aaaaaaatcg tcaaaaagta tttaataatt
     2521 tagctaatct atttgaattt gtgaaagagt tgggaagttt gtttggagat gctatttata
     2581 gttggcagga atcggaagat tggacggaac aagaaaaatt ttatatggaa caagagcttt
     2641 tagggatagg tgtcagcaaa catccactac aagctattgc aagtaaggct atttacccga
     2701 ttaccccaat cggaaatttg tcagaaaata gctatgctat tatcttggtt gaagttcaga
     2761 aaataaaagt gattcgtacc aaaaagggtg aaaatatggc cttcttacag gcagatgata
     2821 gtaagaaaaa attggatgtc actctctttt cagacttata tcgtcaggtt ggacaggaaa
     2881 taaaagaggg agccttctac tatgtaaaag gaaaaataca atcacgtgat ggccgtctgc
     2941 aaatgattgc acaagaaata agagaagcag ttgctgaacg cttttggata caggtgaaaa
     3001 atcatgaatc ggatcaagaa atttcacgca ttttagaaca atttaaaggc ccaatcccag
     3061 tcatcatccg gtatgaagag gaacagaaaa ccatcgtttc tccccatcat tttgtagcta
     3121 aatccaatga attagaggag aaattgaatg aaatcgttat gaaaacgatt tatcgctaaa
     3181 aatacggaaa atagaagaat tttaaaatca aatgtgatat aatcaagaag aatgttaaaa
     3241 gaaaaaggag cataaaccaa tatgaaacgt attgctgttt tgactagtgg cggagacgca
     3301 cctggtatga acgctgccat ccgtgcagtt gttcgtcaag caatttcaga aggaatggaa
     3361 gtgtttggta tctatgacgg atatgctggt atggttgccg gtgaaattca tcccctagat
     3421 gcagcttcag taggggacat catttctcgt ggtggtactt ttcttcactc agctcgttat
     3481 ccagagttcg ctcaacttga agggcaactt aaagggattg agcaattgaa aaaacacgga
     3541 attgaaggtg tagttgttat cggtggtgac ggatcttacc acggcgctat gcgtttgact
     3601 gaacatggct tcccagctat tggtcttcca ggtacaatcg ataacgatat cgttggtact
     3661 gactttacaa tcggttttga cacagcggtt actactgcca tggacgctat cgataagatt
     3721 cgtgatacat catcaagtca ccgtcgtact tttgtaatcg aagttatggg acgtaacgct
     3781 ggtgatatcg ctctttgggc tggtattgca actggtgctg atgaaatcat catccctgaa
     3841 gcaggcttca agatggaaga tatcgtagca agcatcaaag ctggttatga atgtggtaaa
     3901 aaacacaata tcatcgtctt agctgaaggt gtgatgtcag cggctgaatt tggtcaaaaa
     3961 cttaaagaag atggagatac aagcgacctt cgtgtaacag aacttggaca tattcaacgt
     4021 ggtggttctc caactgcgcg tgaccgtgtt ttggcgtcac gtatgggtgc acatgctgtt
     4081 aaacttctta aagaaggtat cggtggtgtt gcggttggta ttcgtaacga aaaaatggtt
     4141 gaaaatccaa ttcttggtac tgcagaagaa ggggcattgt ttagccttac tgcagaaggt
     4201 aagattgtgg ttaacaaccc gcacaaagcc gatattgagc tatctagctt gaataagagc
     4261 ttgtcataat ttacaattta gttaataaga ccaaaaaggt catatatata aaggagtcac
     4321 aaaaatcatg aacaaacgtg taaagatcgt tgcaactttg ggtcctgcgg tagaaatccg
     4381 tggtggtaaa aaattcggtg aggatggata ctggggtgaa aaacttgatg ttgaagcttc
     4441 agctaaaaac attgctaaat tgattgaagc tggtgctaac acattccgat tcaacttctc
     4501 acacggcgac caccaagaac aaggtgagcg tatggcaact gttaaacttg cggaaaaaat
     4561 tgcaggtaaa aaagttggtt tccttcttga tacaaaagga cctgaaatcc gtacagaatt
     4621 gttcgaaggt gaagctaaag aatattcata caaaactggt gaaaaaattc gtgttgcaac
     4681 taaacaagga atcaaatcaa ctcgtgaagt gattgcgttg aacgttgctg gtgctcttga
     4741 tatctatgat gatgttgaag ttggtcgtca agttttggtt gacgatggta aacttggtct
     4801 tcgtgtggtt gctaaagatg atgcaactcg tgaatttgaa gttgaagttg aaaacgatgg
     4861 tatcatcgct aaacaaaaag gtgtgaacat ccctaacact aaaattcctt tcccagctct
     4921 tgctgaacgc gataacgacg atatccgttt cggtcttgaa caaggtatca acttcatcgc
     4981 aatttcattc gtacgtactg caaaagatgt gaacgaagtt cgtgcaatct gtgaagaaac
     5041 tggaaacgga catgttcaat tgttcgctaa aatcgaaaac caacaaggta tcgataactt
     5101 agacgaaatc atcgaagcag ctgatggtat tatgattgct cgtggtgata tgggtatcga
     5161 agtaccgttc gaaatggttc cagtttatca aaaaatgatt atcaagaaag tcaatgctgc
     5221 aggtaaagtt gttatcactg caacaaacat gcttgaaaca atgactgaaa aaccacgtgc
     5281 aactcgttca gaagtatcag acgtattcaa cgctgttatc gacggaactg acgctacaat
     5341 gctgtcaggc gagtctgcaa acggtaaata cccactcgag tcagtaacta caatggctac
     5401 aatcgacaag aacgctcaag ctcttcttaa tgaatacgga cgtcttgatt cagattcatt
     5461 tgagcgtaac tctaagacag aagtaatggc ttctgctgtt aaagatgcta ctaactcaat
     5521 ggatatcaaa ttggttgtaa ctcttactaa gacaggtcat actgcacgtt tgatttctaa
     5581 ataccgtcca aatgctgaca tcttagcatt gacatttgac gaattgacag aacgtggctt
     5641 gatgttgaac tggggtgtta tcccaatgtt gacagatgct ccatcttcaa ctgacgatat
     5701 gttcgaaatc gctgaacgta aagcgataga agcaggtctc gttgagtcag gcgatgatat
     5761 cgttatcgtt gctggtgtgc cagtaggaga agctgttcgc acaaacacaa tgcgtatccg
     5821 cacagtacgt taagaaaaat ataaaaacct atcatatcca gctttagagc ttgtgtgata
     5881 ggttttttgt atagagggta acaaagaggc aaaactttca taatcgattg aaataagata
     5941 tgcacaaatt gattagaaag tcaaatgaat ttctacaaat gttttagcaa tcgtaatgta
     6001 cttgtctaga ttcgatctga tatattttcg atttaatgat atggtattta aaacctccaa
     6061 agtagcttgc tccattcttt tacttacgtg agtgtagatg ttatttactg ttttagcgtt
     6121 tttgtgttcc actctaacca ttatagcatt cttctcagct agtgtactaa ggagtgtgtg
     6181 cctgaaaata tgggaactaa ggggctggtt tatcggtttc tctagtttag tatttgcctt
     6241 ttgcaaagtg atcttaaatg cctttctcta aatttacata tcactattgt ttaacaaaat
     6301 ctaatctatt ttaggtcact tattcttttt ttgaaatgta gaatgaactt tttcaaagtt
     6361 tttcgaatct tttaaaatct gtttgtttta tatcgccatt ctcccccctt ttttaattct
     6421 ccctatatag cctgacaact ttcccgatgg tacgaatatg gttgctttcg tctaggtgga
     6481 tgtcggggta ttcgggattg agtttttttg aggtagcctt ggcggagttt cttgacatag
     6541 ttagcgccgt ctacttggaa gatgccgatg gtattatagt caatctgtgg ggtattcttg
     6601 ataaataggt agtcgctgtt tcttatcttt gcctccatgg acttgctgac gacataagcg
     6661 attgggtcgt agtcgtctgg gataatggaa actccataac taaatcgtcg tcctgcatcg
     6721 agcggctacc tgcagagata aactacctaa cacgagagta agtagtctgt ctgtagtcgt
     6781 ccagtctgat gatttttacg atccttcgtt tttctgctta tacagttgcc tctcggcata
     6841 ggttaggact ttaccttgtc tgggtggtgc ccgttggtcg tagatagatt ggatatcgct
     6901 aggagaatcc ttttgaactg gaggaaagag ggcatcaatc aagctactga atactttaac
     6961 taagtcaaat atagtatttt tcttagtaga cctaaccctt ttttcataat ttctaatggt
     7021 gtttttactt atacctatct tagtacccaa ttcttattga gtccaaccat tactagtcta
     7081 tattgtttta tagttgattg agtttggaat agtacgctgt agctgctaaa acatttctag
     7141 gaattaattt gcctttccta atagagttgt tcatatctta tttcaatcta ttatattttt
     7201 cgcctctaac aatcgcaatc tcttctttat ctacgaacga aatcgctttc tatttttgta
     7261 agtaaagcat cacacgaaat ccacgaaaat gaaaaccttt gttgtgtttt cgtaaaaaat
     7321 ttgttgacag agcacgaaac gcctactata attaaatcaa gtttaaggaa ataacaaaaa
     7381 aacaaatcgg agggaaagac gatgaacaca ttaaatgaga aagtaatcaa tatctgtaaa
     7441 gcagtagtta aagaaacttt aatccaagac atttaggaga aagaaatgaa gaaatcaaga
     7501 aaattagcca cattaggaat ctgttcagcc ttgtttttag gcttggctgc ttgtcaacaa
     7561 caacatgcta cttctgaggg tacgaatcaa aggcaaagca gttcagcgaa agttccatgg
     7621 aaagcttcat acaccaacct aaacaaccag gtaagtacag aagaggtcaa atctctctta
     7681 tcagctcact tggatccaaa tagtgttgat gcatttttta atctcgttaa tgactataat
     7741 accattgtcg gctcaactgg cttatcagga gatttcactt cctttactca caccgaatac
     7801 gatgttgaga aaatcagtca tctctggaat caaaagaagg gcgatttcgt tgggaccaac
     7861 tgccgtatca atagttattg tcttttgaaa aattcagtca ccattccaaa gcttgaaaag
     7921 aatgaccagt tgcttttcct agataatgat gcgattgata aaggaaaggt ctttgattca
     7981 caagataagg aagagtttga tattctattt tcgagagttc caactgaggc aactacagat
     8041 gtcaaggttc acgctgaaaa gatggaaaca ttcttctcac aatttcaatt caatgaaaaa
     8101 gctcgaatgc tgtctgtagt cttgcacgac aatctggatg gcgagtatct gtttgtaggc
     8161 cacgttgggg tcttagtacc tgctgatgac ggtttcttat ttgtagagaa attgactttc
     8221 gaagagccct accaagcgat taaatttgct agtaaggaag attgctacaa gtatttgggc
     8281 accaagtatg cggattatac aggcgaggga ctggctaagc cttttatcat ggataatgat
     8341 aagtgggtta aactttaata gtgttatgat atgacagtaa tcttaggatt tttatttcta
     8401 tttttgataa gttggtgggt tatttcaatc gttaatagcc agaaaccacg caaaaatgaa
     8461 acatttattg gctatgtcca aagatacgat attgatggga atggctatca atcattaccg
     8521 tgtcctcaga actgagagga gttcttgaaa tcgtaaaacc actttgaaca agagttactt
     8581 caacccattg gctccgacgg attaagttgc tttcgtgaat accaaaatca gccgcaattt
     8641 gttcataagt gcggtattct cgcacatatt gaagagtggc cataagaaga tcttctaggc
     8701 ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg ataagctgtt tttaatacag
     8761 ctaatatctc ttcaaaagtc gtgcgctgaa caccaacaag acgtttaaat cgtgcatcag
     8821 ttagttgttt acttgcttca taattcatag aactactata ccatattttg tttcgcagga
     8881 agtctaatgc taaaatctta tcagtgagaa gatttcttaa agtttattct ttgtagtttc
     8941 atctgtatta gtacataatt taacttagag aaaataggag gactaatcga atggaattaa
     9001 aagattttac agaaaaagaa caggaaatga ttaagaaaag gcttacaatg tctaatatta
     9061 gtgacaaaga aactactgag aagattctgg cgctagtccc acaagacttg attaagcgaa
     9121 tcccgttttt tgtcagaaaa catgctacaa cacgtacgat taaacgcatt tcaattgaat
     9181 accctgaact ctacgctgta gctcaaacaa gtggtgagat tccagaaaaa aaaacgcgaa
     9241 gaattgcgtc agattatcac aactatcttt gaacaaaaga tgaataagca tagtattaag
     9301 tagagaatga aaaaatattt tattggcggt ttgggaagca atgcctatca tagcaaggat
     9361 tttcttcaag aactagattc gcaggtctat tttctaaatc catatgaaaa gcatcttcga
     9421 gatgaaacag aattgaaatc atggtttaaa aatgagattg tagaggaaga atctatctgt
     9481 ctgataggtc attctattgg aggcgattta gctcgttatt tcgcatcgga atttgaagaa
     9541 gtaaagaaac tgattctttt ggatggtggc tatctagatt tagataagat tttacctttg
     9601 gatacagagt tagaggaaac taaaaattat atcaaatctc aaattatttc ggacttagat
     9661 gttcttactt ctaaagaaaa atctgaagca aagcattggt cagaaaatat ggagaaagct
     9721 gtaagacagt cctatcactg gaatgttgag tataatagat atgagttggc tataaattat
     9781 gaaaatatag aagcgatact ccgcctacgg agaaaaatac aagcttttaa gagagaagtg
     9841 ggagatacct tgtttattag tcctcgctat cctaatgaag ctacatggag agaggaagcc
     9901 ctaaaagaat tgccagacta ttttgatact atttttttag aaaactttgg ccatgagctt
     9961 tatactcaag cacctaaaga aatcgctagt ctaatgaatg agtggctcgc ttattttcta
    10021 tgagctactt gtattgctgg aaatggtata agatgaaaat ctacaagtca ctaattttca
    10081 tataaccaaa aaacgaacag gctctttttc tgttattcag aagagaactt gttcgttttt
    10141 atcttgattt tcttaaaaag ctctatatac atagggattt tctggtttat cgactttggt
    10201 aaagctgtcg atttccaagg ttggaaggtt ttgtttgagt tctttatggt agtgattgac
    10261 cagttttcct ttggctgaaa tccaagtgtt gttttcatac tgccgggtat tgcccttggt
    10321 cagcaatcca tagacaccag aatctgcgat gcagtgaata atgccgaagc ggaacagaaa
    10381 ttggctatta gcatgactgg ggtcattata gacaaagcct gtgaactgga ttgtcttacc
    10441 ctcaaattca tctggatagt cgtagatagc ctccatgacc tccatatagt tttcattagt
    10501 gatctgaata ctatcttggg ataagtattt atccgccgcc gttcgcattt ccttttcata
    10561 ggctgatttt gaaaaataag aactggtatc tggtttcaaa tattggcttg tcgtcccttc
    10621 gcttgtctga atggctagat ccgttccttc cgataggggg aaatgataac ctttagcaga
    10681 aacggtctga gaatccaagc taacagttgg gaaagttaag ccgatgacaa gtggaatagc
    10741 cagaagagaa atactcgtca tcttggctaa tcggttgttc agatgactgt gggttttgac
    10801 ttgcttcatc cagatataca attgaacgat agccaagata aaagaaagca ccatggagat
    10861 ataggccaga taggaatagt gcatgttgat gtactggttt agtttgcccg acagatggag
    10921 gtaaatagtc agttcaaaat agccagctaa aactaaaaat cgaatcatag gattactcca
    10981 atcaagagag aatagactaa gacgacaaga gttacaattg ttatgaagtg actgataaat
    11041 cgtgctttca agtaattttt catcatgaga atatttttga tatccagcat tggaccaatg
    11101 acgagaaagg ccagaactgg tgccaaaccg aaactcgaga gaagagaagc acctataaag
    11161 gcatccgcct cactacagag cgaaagaaga aaggctaaaa tcatcaagag caggatggca
    11221 aaaagagggg tcgcactgat agaggtcaga atccgagtcg gaacgtagac ctgtattata
    11281 gaagcaaaga ggcagccaaa taccaaataa cgccccgtat caaaaaattc atcaatggcc
    11341 tgcacaaaga cttgaaaaac tttttttgca gaactcaagt aagaaaaatc atgctcatga
    11401 caagccagac gattttcttt ctgaatcggt tcttgccaga aaaatcctag aaaaattcct
    11461 agtattacag ccacaagaat ggaacccaga gctcgtaata gggcgacatg gaaggagttg
    11521 ccaaaggcag aataggtcgc aaaaagaaca atgggattga taacaggtgc tgtcacaaga
    11581 aaaggaacgg ccgtgtaact tggaaccttt ttttccagaa aacgattgat gatggggacg
    11641 attccacatt cacaagaagg gaaaaacata ccgacgaagg tcccaaaaaa gattctcccc
    11701 caacgatttc gagggagaaa atgataaacc ttgtcaggtg tgatataaac ttcaatcagc
    11761 cctgagacaa tgcttcctat cagaacgaag ggaagggctt caatgataat ggagagaaaa
    11821 atggctccag tttgaagtac actagatggg agttgttgaa aagcagtcat ctagtttttc
    11881 ttttctgctt tggctttttt ctctttgtca tcagggaaag taacttgttt caaatcaggg
    11941 agtttagcaa actcttcaaa catcttgtcc aagtcgtctc gttttgtaaa ttttacagcc
    12001 ataggtgtac ctcgattcta aattctacct ctattatact attattttgc ggaaaaggga
    12061 gttttttaat ttgttttcga tagttgatac tcttcgaaaa tctcttcaaa ccacgtcagc
    12121 ttcgccttac tgtagtacgg ttactgactt cgtcagttct atccacaacc tcaaaacagt
    12181 attttgagca gcctacggct agcttcctag tttgcttttt gatttttatt gagtatgaaa
    12241 aggattggag aaagccttcc agcttgtggt ataatagatt tatggataaa aaatatgaaa
    12301 aaatctctca ggatttggga gtgacgttaa agcaaattga taccgttcta agtttgacag
    12361 ctgaaggggc gactattccc tttatcgcgc gttatcgcaa ggacatgact ggtagtctgg
    12421 atgaggtggc gattaaggct attattgatt tggataaaag tctgacaaat ctcaatgacc
    12481 gtaaggaagc tgtcttagct aagattcaag aacaaggtaa gttgaccaag gaattggaag
    12541 aagctatctt agttgccgaa aaattagcag acgttgaaga actctatctt ccttataagg
    12601 aaaagcgtcg taccaaggca accattgccc gtgaagctgg actctttcct cttgctcgct
    12661 tgattttgca gaatatagtt gacttagaga aagaagctga aaagttcgtc tgtgaaggat
    12721 ttgcgactgg caaggaagcc ttgaccggtg cagttgatat tttggtcgaa gccttatcgg
    12781 aagatgtgac cttgcgttct atgacttatc aggaagtgct gagacactct aaactcactt
    12841 ctcaagccaa ggatgaaagt cttgatgaaa agcaggtttt tcagatttat tatgattttt
    12901 cagagacagt tggaactatg caaggctatc gtaccttggc tctcaatcgt ggggagaaac
    12961 ttggtgtctt gaagatcggt tttgaacatg cgacggaccg tattcttgcc ttctttgcta
    13021 ctcgtttcaa ggtgaaaaat gcttatattg atgaagttgt tcagcaatcc gttaagaaaa
    13081 aggtcttgcc tgctattgag cgtcgtattc ggacagaatt aactgagaaa gctgaagagg
    13141 gagctatcca acttttttct gacaatctgc gcaatctcct cttggttgct ccactgaaag
    13201 ggcgcgtggt tcttgggttt gacccagcct ttcgtacagg tgccaagcta gctgtcgtgg
    13261 atgcaacagg aaaaatgctg acaactcagg ttatttatcc tgttaaacca gcatcagctc
    13321 gtcaaatcga agaagccaag aaagatttag cagatttaat tggtcaatac ggtgtagaga
    13381 ttattgccat tggaaatgga acggccagtc gtgaaagtga agcttttgta gcggaagttc
    13441 tgaaagattt ccctgaagtc agctatgtta tcgttaatga aagtggtgct tctgtctatt
    13501 ctgccagcga acttgctcgt caggagtttc cagacttgac cgttgaaaaa cgctctgcca
    13561 tttctatcgc ccgtcgtttg caagatcctc ttgcggaatt ggtcaaaatc gatcctaagt
    13621 caattggtgt cggtcaatac caacacgatg tcagtcagaa gaaactatct gagagtctgg
    13681 actttgttgt cgatacagtg gttaaccaag ttggtgtcaa tgtcaataca gctagcccag
    13741 ctcttctttc acacgtagct ggactcaata aaaccatttc tgaaaatatc gtcaaatacc
    13801 gtgaggaaga gggaaaaatc acttcacgct ctcaaatcaa gaaagttcct cgtttggggg
    13861 ccaaggcttt tgaacaggct gctggtttcc tccgtatccc tgaaagtagc aatatccttg
    13921 ataatacagg agttcaccca gaaaattacg ctgccgttaa ggaactcttc aaatgcttgg
    13981 acattaagga tctgaatgaa gaggcccaaa gtaaactcaa gtcgctttca gtcaaggaaa
    14041 tggcgcaaga gttagacctt ggtccagaaa ctcttaaaga tatcattgca gatcttctca
    14101 aaccaggtcg agatttccgt gattcctttg atgctccagt tctccgtcaa gatgtcctag
    14161 atatcaagga cttagtggtt ggacaaaagc tagaaggagt ggtgcgtaat gttgttgatt
    14221 tcggtgcttt tgttgatatc ggaattcatg aggacggtct catccatatt tcccatatga
    14281 gtcgcaagtt tatcaaacac cctagtcaag tggtggcagt tggagatttg gtgagcgttt
    14341 gggttaagaa aattgatact gaacgtgaaa aagttaatct gtctctccta gctccaaatg
    14401 aaactgactg attatgtcaa gcaggtttca ctagaagact tcggcagacc tttcatccat
    14461 catgtccagt ggaataggcg tctacgttcg acaggtgggc gatttttccc caaagatggg
    14521 catctggatt ttaatcccaa ggtttatcag aaactgggta tggaggtttt taggaaaatt
    14581 gtccgacatg aactctgtca ttatcacctt tatttccaag ggaagggcta tcaacacaag
    14641 gatcgggatt ttaaggaact gttgaaagca gtggatggat taaggtttgt accatccttg
    14701 cccaatagta actctaaacc actcaagctt tatcgttgtc aatcctgcca gcaaaggtat
    14761 cagcgcaagc gtaggattga taccaaacgc tatcgctgtg gactttgtcg aggtaaattg
    14821 cttctgataa atcagcctga ggactgatga aagccgagcc tccccgtgat atactatgtc
    14881 taacctagca gaaagaggaa atatcatgaa cagtaaattt tataaaatga gacgcaatcg
    14941 catgatttca ggagttctgg caggtctgtc agacaagtgg aattttgatg ttaccctagt
    15001 tcgtttcttg tttgccattt ttacagttgc aaactttgga cttggcgtga ttatctatat
    15061 catcctagct tctatcatgc ctaccaagga agaaatcgaa gcagaaatgt acggaacagg
    15121 cccacgtaaa cgcaaagaag cccaagccat tgacgacaat gaaggctggt tttggtgagg
    15181 ttctacagaa gatttggaat aagcaaaaag ctttaaatca atagtctaaa ctgcttgatt
    15241 taaagctttt ttacttttat actcaataaa aatcaaagtg caaactagga agccagccac
    15301 aggctgtaat ttagtcggca atgtgaagtt gacatagttt gcactttgat ttttgaatag
    15361 tattaataat atttcgccag cttcatccac aaactatagt ggattggaac tagaatagga
    15421 cacctctact tctaaaacat tgttagaaat cgatttgact atcctgatcg atttgtcctg
    15481 ttcttatttc attttattat aaaagcagta atttaaactc agttttttaa tctatttttg
    15541 ctgctgagac aatacatttt tggaaaaatg gtggatgctg ataacaactg ataaccagca
    15601 aataacataa gcagtgccgg taaattctgt accgataaag tgtgtgtgtt caactaagag
    15661 ctgaccgatg aatggagaga gcagatagag aagtccgatg agtatatgtt ctttgtgttt
    15721 cattaaagcg caccttcttt ccttataggt tgaacgactt gcaagtgtta aattgaatgg
    15781 tatgttatct aggtaacctt actaaaatgt tagttcccgc ttcctccttt tcaaagtaag
    15841 ccttttcaca cttatatttt acaactttaa aaataggaaa tcaaatcatt ttctaagtaa
    15901 ttttgcttat atttcaccca atcttacaaa aatgtaagat taaagtctct tttgtaaggt
    15961 taggttatgg caagcgatct ttttttgatg taaactagac tcataaagaa caaaggagta
    16021 acatcatgaa acaaatctta cataagaaaa ctagaaaacc aagtcacaaa gaaattgagc
    16081 gcgttcagct gggatgcact atgatgcaag ctacatttca tttgatggga tattaagaaa
    16141 ggagattctc atgacacttt tagatgtaaa acacgttcaa aaaatttata aaacacgttt
    16201 tcagggcaac caagtagaag ccctcaagga tattcacttt accgtcgaaa agggtgacta
    16261 cgttgccatc atgggtgagt ctggttctgg taaatcaact cttctcaata ttctagctat
    16321 gctggataaa ccaagtcgtg gtcaggttta cttgaatgga actgacaccg caaccattaa
    16381 aaattcacag gcctctagct tccgtcgtga aaagttgggc tttgtcttcc aagactttaa
    16441 cttgctagat actctatctg taaaggacaa tatcttgctt ccgcttgtct tgtcaagaag
    16501 acctattaca gagatgatga agaaattggt ggtgacagct gagaatctgg gaataaacca
    16561 attacaagag aagtaccctt acgagatttc tggtggccag aaacagcgtg tagcagtagc
    16621 acgcgccatc atcactgaac ctgaaattct ccttgcggac gaaccgacag gagctcttga
    16681 ctccaagtca tctgcagcct tacttgatgt ctttgatgaa atcaatgagc gcggccaaac
    16741 cattctcatg gtaacccact caacagcagc agctagcaga gctaaacgtg tactctttat
    16801 caaagacggc attctttaca atcaaatcta ccgtggagag aagacagaac gtcagatgtt
    16861 ccaagaaatc tctgatacct tgactgtcat ggcaagcgag gtgaattagt atgtttcgat
    16921 taaccaataa gttagcggta tcgaacttga ttaaaaaccg caaactctac tatccttttg
    16981 cgctggctgt tctcttggca gtcactgtca cctatctctt ttactctcta accttcaatc
    17041 ctaagattgc ggaaatccgt ggaggaacaa ccattcaggc tacacttgga tttggtatgt
    17101 ttgtcgtcac ccttgcgtca gccattatcg ttctctatgc caatagtttt gtcatgaaga
    17161 accgttccaa ggaactagga atttatggca tgttgggctt ggagaagcgt catcttatca
    17221 gtatgacctt taaggagtta gtggtatttg ggattctaac tgttggagcg ggtatcggta
    17281 ttggagcctt gtttgacaag ttaattttcg ctttcctgct caaactaatg aaattgaagg
    17341 ttgagctggt tgctaccttc cagacgaaag ttgtcattac agtgcttgtt gtcttcggtt
    17401 tgattttcct aggcctcatg ttcctgaatg cccttcgaat cgcccgtatg aatgccctcc
    17461 agctctctcg tgagaaagct agtggagaga aaaaaggtcg cttccttcct ctccaaacca
    17521 ttcttggttc cataagttta ggaattggct attatcttgc ccttacggta aaagatcctc
    17581 ttacagcctt aacaaccttc ttcatagctg ttttactggt tatctttggg acttatctct
    17641 tgtttaatgc agggattacc gttttcctcc aaatcttaaa gaaaaataag aaatactatt
    17701 accaaccaaa taacctcata tctgtttcta acttgatttt ccgtatgaag aaaaatgcag
    17761 ttggactagc taccatcgct atcttgtcaa caatggtttt ggtaaccatg tcagcagcga
    17821 caagcatttt caattcctca gaaagcttta aaaaagttct aaatcctcat gattttgggg
    17881 tttcagggca aaatgttgaa aaagaagact tggacaaact cttgagccag tttgcaagtg
    17941 acaaaggtta taagattaaa gaaaaagaag tatttcgtta cacttacttt gctgttgcga
    18001 atcaagaagg aaccaagtta actatttttg aaaaaggaca aaaccgtgtc caaccaaaaa
    18061 cagttttcat ggtatttgac caaaaagatt atgaaaatat gactggtcaa aaactgtctc
    18121 tatcaggaaa tgaggtcgga ctctttgcaa agaatgaggg agttaaagaa cagaaagctc
    18181 taactctaaa tgatcatcaa ttttctgtaa aagaagaatt tactaaagat tttattgtca
    18241 accatgttcc aaatcagttt aatattttga ctgctgatta caattacctt gttgtacctg
    18301 atttacaagc ctttttggat caattcccag attcggctat ctataatcag ttttacggtg
    18361 gtatgaatgt aaatgccagt gaagcagaac aactcaaggt cgctgaggag tatgaaaaat
    18421 acttacaaaa gtttaatgct caattaaaca ctgaaggtaa ctatgtgtat ggtagcactc
    18481 tagcagatgc tagtgctcag atgagtgccc tctttggtgg tgtcttcttt atcggtattt
    18541 tcttatccat tatctttatg gtcggaaccg ttctggtcat ctactacaaa caaatttctg
    18601 aaggatatga agaccgtgag cgctttatta tcttgcagaa agtcggttta gatcaaaagc
    18661 aaatcaagca aaccatcaac aaacaggttt taactgtatt cttccttcct ttgctctttg
    18721 ccttcctaca tctagccttt gcctaccata tgcttagtct gattttaaaa gtgattggtg
    18781 tactggatac gactatgatg ttgattgtga ccttgtctat ctgcgctatc ttcctcatcg
    18841 cctatgtgct gattttcatg attacttcaa gaagttatcg taagattgtc caaatgtaaa
    18901 aaaagatacc tcgacttcaa aatcgaggta tttcttgttt tctaaatgct gaaaagttgt
    18961 ccgagcagga aggtaactcc catagttaag agaccaatag caaggttccg aatcatagct
    19021 gttttggttg gggcttttcc aagtctagca cttgtgtagc cagtgagaag aagggctaca
    19081 ccgacgataa ggacggtagc agggatgcgg taatcacttg gaaaaatggt cactgacagc
    19141 attggaggca aacttctaag gaaaaaggca acgaagctag aaatggcagc gtgccaagga
    19201 ttggtaaatt cttcatactc aatcccatat ttttcctcta ccagagcctt gagtggattt
    19261 ttaagaaaga tcttattagt caagagttgg gcagaagttt tgaattctcc attttggata
    19321 taagaagcat agagggattt tttggctagt tccctatctt ggtctagcaa gagtttttct
    19381 cgcgaaacgg cagcttcctc ggtatctttt ggagttgaaa cggatacata ttctccacca
    19441 gcaatggaaa taattccatc gttagcatca agaacacccg cacgcaggat atttaaacga
    19501 cctgcaaaat ttgaatcaat ttcgtgattt gtttctgacg ctaaatttca agttcaagtt
    19561 agccatccag aagtcttctc tgggtgactt gtagtccaag cattttttag gatagttgtt
    19621 aatccacttt tcgatgaatg cgacttcttt gggagtcatt ttcttggttc ccttaggtaa
    19681 ccatctacga atgagcctgt tgtgattctc attagttccc ctttcccaag aggcataggg
    19741 gtgcgcataa tagatgtgct cctcagaaaa tacatcaaac aagcgattga attccgttcc
    19801 attatctgcc gtgatggaaa gaatcttgtg ttgttttaag atgagtttta gagcctgatt
    19861 gaccacctca gcacttttat ttggaatcaa tcggatgatc tgatgtctac tctttcgatc
    19921 cgtcaagaca agcaagcagt agtttttcgc tctcgtaagt aaccgtatca atctcataat
    19981 gcccattctc caagcgaaga ttgatagctt cagaccgctg ttcgatggat tgaccagc
//
LOCUS       DAVHIT010000033        19323 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVHIT010000033 DAVHIT010000000
VERSION     DAVHIT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.034"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..147)
                     /locus_tag="VVN16_001046"
     CDS             complement(<1..147)
                     /locus_tag="VVN16_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5572452.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSL"
     gene            1324..2583
                     /locus_tag="VVN16_001047"
     CDS             1324..2583
                     /locus_tag="VVN16_001047"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5572453.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2599..3150
                     /locus_tag="VVN16_001048"
     CDS             2599..3150
                     /locus_tag="VVN16_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572454.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            3143..4420
                     /locus_tag="VVN16_001049"
     CDS             3143..4420
                     /locus_tag="VVN16_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5572455.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4436..5296
                     /locus_tag="VVN16_001050"
     CDS             4436..5296
                     /locus_tag="VVN16_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU5572456.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5677..6481
                     /locus_tag="VVN16_001051"
     CDS             join(5677..6045,6047..6481)
                     /locus_tag="VVN16_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU5572457.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMSTLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     SHDMKLFKMSCRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            6673..8964
                     /gene="pcrA"
                     /locus_tag="VVN16_001052"
     CDS             6673..8964
                     /gene="pcrA"
                     /locus_tag="VVN16_001052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU5572458.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            9007..9687
                     /gene="radC"
                     /locus_tag="VVN16_001053"
     CDS             9007..9687
                     /gene="radC"
                     /locus_tag="VVN16_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU5572459.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVESLSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9684..10373)
                     /locus_tag="VVN16_001054"
     CDS             complement(9684..10373)
                     /locus_tag="VVN16_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5572460.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(10391..11032)
                     /locus_tag="VVN16_001055"
     CDS             complement(10391..11032)
                     /locus_tag="VVN16_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU5572461.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(11115..11330)
                     /locus_tag="VVN16_001056"
     CDS             complement(11115..11330)
                     /locus_tag="VVN16_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU5572462.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(11338..11685)
                     /locus_tag="VVN16_001057"
     CDS             complement(11338..11685)
                     /locus_tag="VVN16_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU5572463.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(11690..12805)
                     /locus_tag="VVN16_001058"
     CDS             complement(11690..12805)
                     /locus_tag="VVN16_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5572464.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(12815..13774)
                     /locus_tag="VVN16_001059"
     CDS             complement(12815..13774)
                     /locus_tag="VVN16_001059"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU5572465.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(13887..14456)
                     /locus_tag="VVN16_001060"
     CDS             complement(13887..14456)
                     /locus_tag="VVN16_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU5572466.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            14584..15255
                     /locus_tag="VVN16_001061"
     CDS             14584..15255
                     /locus_tag="VVN16_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU5572467.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            15239..16057
                     /locus_tag="VVN16_001062"
     CDS             15239..16057
                     /locus_tag="VVN16_001062"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU5572468.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            16054..16950
                     /locus_tag="VVN16_001063"
     CDS             16054..16950
                     /locus_tag="VVN16_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5572469.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     DIRLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLDDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            16994..17968
                     /gene="pta"
                     /locus_tag="VVN16_001064"
     CDS             16994..17968
                     /gene="pta"
                     /locus_tag="VVN16_001064"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU5572470.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(18283..19323)
                     /locus_tag="VVN16_001065"
                     /pseudo
     CDS             complement(18283..19323)
                     /locus_tag="VVN16_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5739 a         3506 c         4221 g         5857 t
ORIGIN      
        1 tagacttcca caatcagggc aagatggagc ctcataatcc agcttagcga taatttcttt
       61 gtgggtatcc atattgatga tatctagaat cttgatgttt gggtctttaa tatcgagcag
      121 ttttgtgata aaatgtaatt gttccatatg aatctttcta atgatggttt tgtcgctttt
      181 cattataggt catatgggac tttttttcta caataaaata ggctccataa tatctatagg
      241 ggatttaccc actacaaata ttatagaacc ggattttgtg gtacgacagg catgtatagc
      301 aaactgaatc tggaatagca cagcatatct tctaaaatat tgttataaat tgatttgaat
      361 ttcaaaatta aattgtttga ttcttatttc aatttgttat agtatatctg atgtcaaagt
      421 tctcggcgag tcaaatagcg attcccaagc ctgactatcg tgaggtagcg gattaaaatg
      481 gtctggggat agaccgtttt aagtctgacg ctggaaataa gaattgtcag aagaagggat
      541 agcgaaatcg tggctctacg aacaggaacg tgataataag gcgtatatag cggataaggg
      601 ggcctcaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgcgtaaa tcacgagagt
      661 aattgaattc gtactaagat tttctatttt cactgtaacc ttttaacgtc cttatatctt
      721 gtatacacga ggaaagatgt acgacttatc ccgtgaggtc tatcactaat aaagagaaaa
      781 cgacagatag aagcgatcct gagtcacggt tatctttaaa atagattgtc tgataggatg
      841 ctagcctgaa aacgtcttgt tcatgaaata tagggacggt atgtataaaa cgcttctgtg
      901 aactgagaga agggggagaa gttcttgata aaatttagtt gaacagccgt attccgatac
      961 ttagataaga gatctagtct tagctcctac tcggtttcag aggataaaaa agggcaatag
     1021 cgattcgaga aagattatac tcttcgaaaa tctcttcaaa tcacgtcaat atcgccttgc
     1081 cgtacgtgta ggatactgac tacgtcagtt ccatctacaa cctcaaaaca gtgttttgag
     1141 caacctgcgg ctagtttcct agtttgatct ttgattttta ttgagtatta gtaattcagt
     1201 tactaactcg tcaactctga tttatccaat aaaattgaaa aggatggaaa aaaggataaa
     1261 tttatgatat actttatttt gaagacctta ttagaaatct tgaaagagta ttgaaaactt
     1321 agaatgagaa aaattgttat caatggtgga ttaccactgc aaggtgaaat tactattagt
     1381 ggtgctaaaa atagtgttgt ggccttaatt ccagctatta tattggctga tgatgtggtg
     1441 actttggatt gtgttccaga tatttcggat gtagccagtc ttgtcgaaat catggaattg
     1501 atgggagcta ctgttaagcg ttatgacgat gtcttggaga ttgatccaag aggtgttcaa
     1561 aatattccaa tgccttatgg taaaattaac agtcttcgtg catcttacta tttttatggg
     1621 agcctcttag gccgttttgg tgaagcgaca gttggtctac cgggaggatg tgatcttggt
     1681 cctcgtccga ttgacttaca ccttaaggcg tttgaagcta tgggtgccac tgctagctac
     1741 gagggagata acatgaagtt atctgctaaa gatacaggac ttcatggtgc aagtatttac
     1801 atggatacgg ttagtgtggg agcaacgatt aatacgatga ttgctgcagt taaagcaaat
     1861 ggtcgtacta ttattgaaaa tgcagcccgt gaacctgaga ttattgatgt agctactctc
     1921 ttgaataata tgggtgccca tatccgtggg gcaggaacta atatcatcat tattgatggt
     1981 gttgaaagat tacatgggac acgtcatcag gtgattccag accgcattga agctggaaca
     2041 tatatatctt tagctgctgc agttggtaaa ggaattcgta taaataatgt tctttacgaa
     2101 cacctggaag ggtttattgc taagttggaa gaaatgggag tgagaatgac tgtatctgaa
     2161 gacagcattt ttgtcgagga acagtctaat ttgaaagcaa tcaatattaa gacagctcct
     2221 tacccaggct ttgcaactga tttgcaacaa ccgcttaccc ctcttttact aagagcgaat
     2281 ggtcgtggta caattgtcga tacgatttac gaaaaacgtg taaatcatgt ttttgaacta
     2341 gcaaagatgg atgcggatat ttcgacaaca aatggtcata ttttgtacac gggtggacgt
     2401 gatttacgtg gggccagtgt taaagcgacc gacttaagag ctggggctgc actagtcatt
     2461 gctgggctta tggctgaagg taaaactgaa attaccaata tcgagtttat cttacgtggt
     2521 tattctgata ttatcgaaaa attacgtaat ttaggagcgg atattagact tgttgaggat
     2581 taaaccgtag aggtgtttat gaatatttgg accaaattag caatgttttc tttttttgaa
     2641 acggatcgct tgtatttgcg tcctttcttt tttagtgata gtcaggactt ccgcgagata
     2701 gcttcaaatc cagaaaatct tcaatttatt ttcccaacgc aggcaagtct ggaagaaagt
     2761 caatatgcac tggccaatta ctttatgaag tcccctttgg gagtgtgggc aatttgtgac
     2821 cagaaaaatc aacaaatgat tggttctatt aaatttgaga agttagatga aatcaaaaaa
     2881 gaagctgagc ttggctattt tttgagaaaa gatgcttggt cgcaaggatt tatgacagag
     2941 gttgttagaa aaatttgtca gctttctttt gaggaatttg gcttaaaaca attatctatc
     3001 attacccacc ttgaaaataa agctagccaa agagttgctc ttaagtctgg atttagtttg
     3061 ttccgtcagt ttaagggaag tgatcgttac acaagaaaaa tgcgggatta tcttgaattt
     3121 cggtatgtaa aaggagagtt caatgagtaa gcatcaggaa attctaagct atttggagga
     3181 attaccagta ggtaaaaggg tcagtgttcg tagcatttcg aatcatctag gagttagtga
     3241 tggaacagcc tatcgggcta ttaaagaagc tgaaaaccgt ggaattgtgg agacccgtcc
     3301 tagaagtgga acaattcgtg ttaaatccca gaaagttgct atagagagat taacgtttgc
     3361 tgaaattgca gaagtgactt cttctgaggt tctggctggg caagaaggtt tagagagaga
     3421 atttagtaag ttttcaattg gtgccatgac tgaacaaaat atcttgtctt accttcatga
     3481 tggggggctc ttgattgtcg gagaccgaac ccgtattcag ttgctagcct tggaaaatga
     3541 aaatgcagtt ctggttacag ggggatttca ggttcatgat gatgtgctta aactggccaa
     3601 tcaaaaaggg attcctgttc taagaagtaa gcatgatacc tttaccgtcg cgaccatgat
     3661 caataaagcc ttgtcaaatg tccaaatcaa gactgatatt ctgacagttg agaaacttta
     3721 tcgccctagt catgagtatg gttttctgag agagacagat acagttaaag attatttgga
     3781 cttggttcgt aagaatcgta gcagccgttt ccctgttatc aatcaacatc aggtcgttgt
     3841 tggcgttgta accatgagag acgctggtga taaatcacca agcacgacaa ttgataaggt
     3901 tatgtctcgt agtctatttt tggttggatt atcgacaaat attgccaatg tgagtcaacg
     3961 gatgatcgca gaagactttg aaatggtacc agttgttcga agcaatcaaa ctttgcttgg
     4021 cgttgtgacg cgacgagatg tcatggagaa gatgagccgt tcccaagttt cggctctacc
     4081 aactttttct gagcagattg gacaaaagct ctcttatcac catgatgaag tagtcattac
     4141 agtggaaccc tttatgctag aaaaaaatgg agttttggct aatggtgtat tggcagaaat
     4201 tctgacccac atgacccaag atttagttgt taatagtggt cgcaatctca ttatcgagca
     4261 gatgctaatc tactttttgc aggctgttca gatagatgat atattgcgca ttcaggcacg
     4321 gattattcat catacgagac ggtcagctat aattgattac gatatttatc atggtcacca
     4381 gattgtttca aaagcaaatg tgactgttaa aattaattag aaactaggag aaaagatgat
     4441 aacattaaaa tcagctcgtg aaatcgaagc tatggacaag gctggtgatt ttctagcaag
     4501 tattcatata ggcttacgtg atttgattaa gccaggcgta gatatgtggg aagttgaaga
     4561 atatgtccgc cgtcgttgta aagaagaaaa tttccttcca cttcagattg gggttgacgg
     4621 tgccatgatg gactatcctt atgctacctg ttgctctctt aacgatgaag tggctcacgc
     4681 tttccctcgt cattatatct tgaaagatgg tgatttgctc aaagttgata tggttttggg
     4741 aggtcccatt gctaaatctg acctaaatgt ctcaaaatta aacttcaaca atgttgaaca
     4801 aatgaaaaaa tacactcaga gctattctgg tggtttagca gactcatgtt gggcttatgc
     4861 tgttggtaca ccgtccgaag aagtcaaaaa cttgatggat gtaaccaaag aagctatgta
     4921 caagggtatt gagcaagctg ttgttggaaa tcgtatcggt gatatcggtg cggctattca
     4981 agaatacgct gaaagtcgtg gttacggtgt agtgcgtgat ttggttggtc atggtgttgg
     5041 cccaactatg cacgaagaac caatggttcc taactatggt attgcaggtc gtggactccg
     5101 tcttcgtgaa ggaatggtct taaccattga accaatgatc aatacaggcg attgggaaat
     5161 tgatacagat atgaaaactg gttgggcgca taagaccatt gacggtggat tgtcatgtca
     5221 gtatgaacac caatttgtca ttacgaaaga tggacctgtt atcttgacta gccaaggtga
     5281 agaaggaact tattaataaa aagtgaaaag actactggaa gtttattttg ataaaaaatc
     5341 cagtagatct tttcataata aaacgcattg tatcaagtgt taggggctga tatcatgcgt
     5401 ttttctgctt ttaagatttt ttccaactct gtttgtaagc gcatcataac aaagggtcta
     5461 ggattcaggg ctctcctcct atatactatt agtaaagtaa aactaagaga ggatatttta
     5521 gtgtcgcagt ctattgttcc tgtagagatt ccacaatatt gtcgttttga ttctaaaaag
     5581 agaaatggaa ttctgtttaa cgttcgtatt gccaatctta aatttacctt ttttagatta
     5641 gacttcctgc gaaataaaat atggtatagt agttctatga atgatgaagc aagtaaacaa
     5701 ctaactgatg cacgatttaa gcgtcttgtt ggtgttcagc gcacgacttt tgaagagata
     5761 ttagctgtat taaaaacagc ttatcaactt aaacacgcaa aaggtggacg aaaacctaaa
     5821 ttaagcctag aagaccttct tatgtccact cttcaatatg tgcgagaata tcgaacttat
     5881 gaacaaattg cggctgattt tggtattcac gaaagcaact taatccgtcg gagccaatgg
     5941 gttgaagtaa ctcttgttca aagtggtgtt acgatttcaa gaactcctct cagttctgag
     6001 gacacggtaa tgattgatgc gacggaagta caaatcaatc gccctaaaaa aagaattagc
     6061 gaatcattct ggtaaaaaga aatttcacgc tatgaaggct caagcgattg tcacaagtca
     6121 agggagaatt gtttctttgg atatcgctgt gaactatagt catgatatga agttgttcaa
     6181 aatgagttgc agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg
     6241 gctcatgaag atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagccgct
     6301 aacagctgaa gataaagcct gtaaccatgc gctatccaag gagagaagca aggttgagaa
     6361 catctttgcc aaagtaaaaa cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa
     6421 acgcttcgga ttacgaatga atttgattgc tggtattatc aatcatgaac taggattcta
     6481 gttttgcagg aagtctattg aggtattgag ctagtttatg aaaaaattgg gtgaaaaagt
     6541 cgagtgtttt agaaacccac agtgtagtat tctagtttca atccactata ttttgctact
     6601 ccccgtaaag tttctatttt ccctgatttc tgatataata gaaatattga cttcaagagt
     6661 aaggaagaga agatgaacgc attattaaat ggaatgaatg accgtcaggc tgaggcggtg
     6721 caaacgacag aaggtccctt gctaatcatg gcaggggctg gttctggaaa gactcgtgtt
     6781 ttgacccacc gtatcgctta tttgattgat gaaaagctgg tcaatccttg gaatatcttg
     6841 gccattacct ttaccaacaa ggctgcgcgt gagatgaaag agcgtgctta tagcctcaat
     6901 ccagcgactc aggactgtct gattgcgacc ttccactcca tgtgtgtgcg tattttgcgt
     6961 cgcgatgcgg accatattgg ctacaatcgt aattttacaa ttgtggatcc tggtgaacag
     7021 cgaacgctca tgaaacgtat tctcaaacag ttgaacttgg atcctaaaaa atggaatgaa
     7081 cgaactattt tggggaccat ttccaatgct aagaatgatt tgattgatga tgttgcttat
     7141 gctgcccaag ctggcgatat gtatacgcaa attgtggccc agtgttatac agcctatcaa
     7201 aaagaacttc gtcagtctga atccgttgac tttgatgatt tgattatgct gaccttgcgt
     7261 ctctttgatc aaaatcctga tgttttgacc tactaccagc aaaaattcca atacatccac
     7321 gttgatgagt accaagatac caaccacgct cagtaccaat tggtcaaact cttggcttcc
     7381 cgttttaaaa atatctgtgt ggttggggat gcggaccagt ctatctacgg ttggcgtggt
     7441 gctgatatgc agaatatctt ggactttgaa aaggattacc ccaaagccaa ggttgttttg
     7501 ttggaggaaa attaccgctc aaccaaaacc attctccaag cggccaacga ggttattaaa
     7561 aataataaaa atcgccgtcc taaaaatctc tggactcaaa acgctgatgg ggagcaaatc
     7621 gtttactatc gtgccgatga tgagctggat gaggctgtat ttgtagccag aaccatcgat
     7681 gaacttagtc gcagtcaaaa cttccttcat aaggattttg cagttctcta tcggactaat
     7741 gcccagtccc gtacaattga ggaagccctg ctcaagtcta acattcctta taccatggtt
     7801 ggcggaacca aattctacag ccgtaaggaa attcgcgata ttattgctta tctcaacctt
     7861 attgctaatt tgagtgacaa tattagtttt gagcgtatta tcaacgagcc taaacgtgga
     7921 attggtctag gtacagttga gaaaatccgt gattttgcaa atttgcaaaa tatgtctatg
     7981 ctggatgctt ctgctaatat tatgttgtct ggtatcaagg gtaaggcagc ccaatctatc
     8041 tgggattttg ccaatatgat gcttgatttg cgggagcagc tagaccactt aagcattaca
     8101 gagttggttg agtccgtcct agaaaaaaca ggttatgtcg atattcttaa cgcccaagcg
     8161 actctagaaa gcaaggcacg ggttgaaaat atcgaagagt ttctttctgt tacgaagaac
     8221 tttgatgaca ccacggatgt gacagaagag gaaactggtc tggacaaact gagtcgtttc
     8281 ttaaatgact tggctttgat tgccgacaca gattcaggta gtcaggagac atcagaagtg
     8341 accttgatga ccctgcatgc tgccaaaggt ctcgaatttc cagttgtctt tttgattggg
     8401 atggaagaaa atgtctttcc acttagtcgt gcgactgaag atccagatga attagaagaa
     8461 gagcgccgtc tagcctatgt aggtatcacg cgtgcagaga aaattctcta tctgaccaat
     8521 gccaactcac gcttgctttt tggtcgtacc aattataacc gtccgactcg ttttattaac
     8581 gaaatcagtt cagacttgct tgagtatcaa ggtctggctc gtcctgcaaa tacaagcttt
     8641 aaggcatcat atagcagtgg tagtatttcc tttggtcaag gtatgagttt ggctcaggct
     8701 cttcaagacc gtaaacgcgg tgctgcccca aaatcaatcc agtcaagcgg tcttccattt
     8761 ggtcaattta cagctggcgc aaaatcagca tctagcgagg caaattggtc cattggtgac
     8821 attgctctcc acaagaaatg gggagaggga actgttctgg aagtttcagg tagcggtgct
     8881 aggcaggaat tgaaaatcaa tttcccagaa gtaggtttga aaaaactttt agccagtgtg
     8941 gctccaattg agaaaaaaat ctaattttcc atccttctca cgaataataa agtgaggagg
     9001 atttttatgt acagtatttc attccaagaa gattcactat taccaagaga aaggctggcc
     9061 aaggaaggag ttgaatcgct tagtaaccaa gagttgctag ctattttact caggacagga
     9121 acacgtcaag ctagcgtttt tgaaattgcc caaaaagtct tgaacaatct ttcaagccta
     9181 acggatttga aaaaaatgac cctgcaggaa ttgcagagtt tgtctggtat tgggcgtgtt
     9241 aaggccatag aattacaagc tatgattgaa ctggggcatc gtattcacaa acacgagact
     9301 cttgaaatgg aaagtattct cagcagtcaa aagttggcca agaagatgca gcaggaatta
     9361 ggggataaaa aacaagagca cctggtggca ctctatctca atactcaaaa tcaaatcatc
     9421 catcagcaga ccatttttat cggttctgta actcgtagta tcgctgaacc gcgagagatt
     9481 ctgcactatg caatcaagca tatggcgact tctcttatct tggtccacaa tcatccttca
     9541 ggagcggtag cgcctagcca aaatgatgat catgtcacta aacttgttaa agaagcctgc
     9601 gaactgatgg ggattgttct cttggaccat ttgattgtct ctcattctaa ttactttagt
     9661 tatcgtgaaa agacagattt aatctaaagt tcattaacga catagtcaaa gagtttctta
     9721 tctttgggac gattttcaaa aagaagttct ggatgccatt ggacaccgag aaaggcgaca
     9781 tcatccgtac tcatgacagc ctcaatgata ccatctttag gatcatgagc cacaactttt
     9841 aaatttggtg ctaagtcctt gatgctctgg tggtggaagg agttgatatg agagatttct
     9901 ccatagattt cttggagaac ggtatctggt tctgttacca agcgttgagt tgtgtactca
     9961 acagaagaat cctgccaatg gtcttcgata tcttggtaca aagttccacc catggcaacg
    10021 ttaaagagtt gggtaccacg gcagacagag aaaatgggct ttttctgttt aatagcttcc
    10081 ttgatgaggg ccagttcgaa gatatctctt tgaaggtgat aatcatcgct atcaatggtt
    10141 ttgggttcgc catagaattt tggatcgaca ttttgcccac ctgtcaagat gagcttgtca
    10201 atcaaactga tatagtggca ggccatttct tgatcaccaa tcggtaggat gatgggaatc
    10261 cctccagcat ctttaacgcc ttcaacaaag ccttttgctg cgtagctcat catgatgtca
    10321 tcatctggat gagttttttc gtttcctgta atcccaataa ctggtttttt cataaaatga
    10381 ttttcgcttt ctaatcctct tttcgcatga agtagaggag ggtttggagt tcacttgtca
    10441 aatcgacata ctgaacgacc acgtcttttg gtaaatgcag atggactggt gaaaaactga
    10501 gaattccttt cacaccagca tcaaccaaga gattagcaac ctcttgtgac ttgacgctgg
    10561 gaacagttag gatagcagtc ttcacatcag catccttgat tttatccttg atctgagaaa
    10621 tcccgtaaat gggaatcccg tcaggagttt gggtaccgac ttcaggatgg tcgtctaggt
    10681 caaaggccat gataatcttc atcttgttac gttcgtggaa gcggtagtgg agaagggcat
    10741 ggcccatatt tccaatacca accagcatga cattggtaat agagttgtca ttgagcaaat
    10801 cggcaaaaaa tgtcattagt tttttgacat catagccaaa accacgacga ccaagttcac
    10861 caaaatagga aaaatcacga cgtacggtcg ctgaatcaat accgatagcc tctgcaattt
    10921 gcttagagtt ggcacgttca atcttttctg catgaaatct cttaaaaatt cgatagtaga
    10981 gagagagtct ttttgctgta gcttttggaa tagcaaactg tttatctttc acaaaatcac
    11041 aacctttcta ttcttctatt ttatagaaac attgtgaaaa aatcaacaaa aataagaaaa
    11101 aactaagaaa aatcttagtt ttgatgtaaa aaatctgcat gagatagaaa acggtagagg
    11161 tctccgacca gcccctgata aacttttttg cccctaaaag tcagagaagt cacataaagt
    11221 gtatctggta aggttacaca tcctgacaaa gtcaacatga gagcctcatg atcctcatac
    11281 ttgagagtac gctctacatg atagcagtcc ttataggtca gttcaaacat tttggctcta
    11341 tctttccgat tttgtaaaga caccacgttc taccaagcta tccatgagga agtagaattt
    11401 ttcctgatga atatggtggt cttctgattt gaaaatatca actagacgaa ggccaaactt
    11461 gtcagtgata ttgattttag cccctgtaag ttccttgtta atgatgattt tgagttggaa
    11521 gccttcaccg ctgtttggca ctttttccaa aaggcgagtc agttcatagt taccaacctt
    11581 agtttcaaaa aaggtgttat ctttgagggt gaatttttta acagaagggc taagagtgta
    11641 atcgtaacga caatttttta actgaatgat tttttcaaat gccatatggc taacctccga
    11701 taatttcttt taaggttttt gcgagggttt gtaggtcttc aacggtattt tgtggcgaca
    11761 aactgatgcg aagggattcc ttcaagcgtt ctgaatttgc gccatacatg gctttaagaa
    11821 catggctgga ttggacaacg cctgcagtac aggctgagcc agtagagatt gaaattccag
    11881 ctaaatctag ccgaaggagt aagaggtcat ttttctgacc aggaaatcca atattgagaa
    11941 cataagggag atgatgtttt cctctattca agtaatactg aatgccctcc agctctgcca
    12001 gaaaggcagt ttctagattt tgtacatgtt gaaaatgttc ttcttgtttt tctaggtctt
    12061 cttttagggc tgcaaccatg cctacgatgg caggcagatt ttcagttcct gcacgttttt
    12121 tctgttcctg atctccgcca tgtagatagg aatcaaagtc catgctagat gcgtagagaa
    12181 aaccgattcc cttaggacca tggaatttgt gggcagaagc agtgagaaaa tcaatgccca
    12241 attcttctga atgaattggg attttaccaa tagcctgaac tgcatcaaca tgataggcag
    12301 cagggtgttg cttgagtatt tggccaattt cagcgatggg aagtaggttt cctgtctcat
    12361 tattggcaaa catagtagaa accaaaatcg tatcgtcacg taaagccttt tgaatttgct
    12421 gggctgtgat ttcttgattt tctggctgga taatggttgc ttcaaaccca aagtgttgaa
    12481 ccaagtaatc aattgtttca aggacagcat ggtgctcgat ggcagttgtg atgatatgtt
    12541 ttccttgttc ttggtgacga agacagtagc caatgatggt agtattattg ccttcagtcc
    12601 caccagaagt gaaaaagata tgttgaggtt ttgtccttag taactgggct agttcctgac
    12661 gggcttctcg caagagtttg ccagcttgac gaccatgacc atgaatacta gaaggatttc
    12721 cgtgggtttc ttgcataacc ttggtcatag ctgaaatagc aactgctgac ataggagtcg
    12781 ttgcagcatt gtccaaataa atcaaagaat caccttattt ctttttattg taggcaaaga
    12841 gtgggctgac tggttttctt tcgtgaatac ggacgatagc atcaccaatt aactcactag
    12901 cagtgatgta gcatacattt ttaggagttt tttcttttgt tgctactgaa tcagtcacaa
    12961 gaatttcttt aatattagta ttgtcaagaa gctcagcagc tccctcgacg aagagaccgt
    13021 ggctagaaac agcataaatt tctgtagctc cttcacgttc aacgatttta gaagcttcag
    13081 agaaggtacg tcctgtattt aaaatatcat caatcaagat agctttctta ccttcaacat
    13141 caccaataat ataaccttcg ttacgagttg catcgtcttg agggtagtcg ataatggcga
    13201 taggagcatc aagatattca gccaggctac gcgcacgttt gacacctgaa tttttagggc
    13261 taacgacaac aacatctgaa ccaagcaatc ctttatcgca gtaatgtttt gcgaataggg
    13321 gaacagtgaa aagattatcc actggaatat caaagaaacc ttgaacctga acggcatgca
    13381 aatcaagagt caggatacga tcaactccag ccttaaccag catattggca actagttttg
    13441 ctgtaagtgg ctcacgagga caagcaatgc ggtcttgacg tgcatagcca aaatatggaa
    13501 ggacaacgtt gatactgtgg gcacttgcac gcacacaagc atcgaccatg attaacaatt
    13561 ccattaggtg gttgttgaca gggaaacttg ttgattggat gatgtaaaca tcataaccac
    13621 ggacactttc ttcgatattt acttggattt ctccgtctga aaattgacgt gatgatagtt
    13681 ttccaagtgg gacaccaaca gcttgggcaa ttttttgtgc aatctcttgg ttagagttga
    13741 gtgcgaaaag tttcatgttt tttctatctg acattataga ccgtcctctg taaactttat
    13801 aaatcctagt tatatttacc ttacatatat gaactgggat ttgtgtattt ttatcttttc
    13861 tattttacca aaaaatggag attatttcag ctatttttca tacttttgac aaatcgaacc
    13921 aattttgaag gagctttttg gtaggaaatc tgatttttct ctaaaaattg tcggaaatcc
    13981 tgtttgcctt gctcatgatt ttccacttca agctccaatt cgtaatctgt catatcaaag
    14041 tatcggctct gatccagtgc catgagacca atagctgttt tcatttcata gcgaagcgtt
    14101 gttagacaac caagaacctg ccagttctta ctttggatac catgtttcgc caattcatcc
    14161 agtactagcc cttgaggaag ttcttcctta ctcagatagt tctcagcatc ttttagttgc
    14221 aatttttggt tgtattccat gtttccaaca ctctgcggga ctttgagtgt caactcagcc
    14281 cagtcttcaa aggttcgaat gcgcatagcg actttctttt ctcgcagttc aaaatcaggc
    14341 gtgtcgatgt agtaatttgt ttgaagaaca ggagtgacac ctgtgaactg gtcttttaga
    14401 cgattgtatt catctttttt caatagtgtt ttcaattcaa tttctaaatg tttcattttt
    14461 cttacctttt tttatcgttg aaagcggatt tatggtataa taagcattgt atttattgta
    14521 tatgaatctg gagaaaaaat caaagatatt tttgacggat aatatgagaa caagggagaa
    14581 tatatgacct tagaatggga agaatttcta gatccttaca ttcaagctgt tggtgagtta
    14641 aagattaaac ttcgtggtat tcgtaagcaa tatcgtaagc aaaataagca ttctccaatt
    14701 gagtttgtga ccggtcgagt caagccaatt gagagcatca aagaaaaaat ggctcgtcgt
    14761 ggcattactt atgcgacctt ggaacacgat ttgcaggata ttgctggctt acgtgtgatg
    14821 gttcagtttg tagatgacgt caaggaagta gtggatattt tgcacaagcg tcaggatatg
    14881 cgaatcatac aggagcgaga ttatattact catagaaaag catcaggcta tcgttcctat
    14941 catgtggtag tagaatatac ggttgatacc atcaatggag ctaagactat tttggcagaa
    15001 attcaaattc gtactttggc catgaatttc tgggcaacga tagaacattc tctcaactac
    15061 aagtaccaag gggatttccc agatgagatt aagaagcgac tggaaattac agctagaatc
    15121 gcccatcagt tggatgaaga aatgggtgaa attcgtgatg atatccaaga agcccaggca
    15181 ctttttgatc ctttgagtag aaaattaaat gacggtgtag gaaacagtga cgatacagat
    15241 gaagaataca ggtaaacgaa ttgatctgat agccaataga aaaccgcaga gtcaaagggt
    15301 tttgtatgaa ttgcgagatc gtttgaagag aaatcagttt atactcaatg ataccaatcc
    15361 ggatattgtc atttccattg gcggggatgg tatgctcttg tcggcctttc ataagtacga
    15421 aaatcagctt gacaaggtcc gctttatcgg tcttcatact ggacatttgg gcttctatac
    15481 agattatcgt gattttgagt tggacaagct agtgactaat ttgcagctag atactggggc
    15541 aagggtttct taccctgttc tgaatgtgaa ggtctttctt gaaaatggtg aagttaagat
    15601 tttcagagca ctcaacgaag ccagcatccg caggtctgat cgaaccatgg tggcagatat
    15661 tgtaataaat ggtgttccct ttgaacgttt tcgtggagac gggctaacag tttcgacacc
    15721 gactggtagt actgcctata acaagtctct tggcggtgct gttttacacc ctaccattga
    15781 agctttgcaa ttaacggaaa ttgccagcct taataatcgt gtttatcgaa cactgggctc
    15841 ttccattatt gtgcctaaga aggataagat tgaacttatt ccaacaagaa acgattatca
    15901 tactatttcg gttgacaata gcgtttattc tttccgtaat attgagcgta ttgagtatca
    15961 aatcgaccat cataagattc actttgtcgc gactcctagc cataccagtt tctggaaccg
    16021 tgttaaggat gcctttatcg gtgaggtgga tgaatgaggt ttgaatttat cgcagatgaa
    16081 catgtcaagg ttaagacctt tttaaaaaag cacgaggttt ctaagggatt gctggccaag
    16141 attaagtttc gaggtggagc tattctggtc aataatcaac cgcaaaatgc aacgtatcta
    16201 ttggacgttg gagactacgt taccattgac attcccgctg agaaaggctt tgaaaccttg
    16261 gaggctattg agcttccatt agatattctc tatgaggatg accactttct agtcttgaat
    16321 aaaccctatg gagtggcttc tattcctagt gtcaatcact ctaataccat tgccaatttt
    16381 atcaagggtt actatgtcaa gcaaaattat gaaaatcagc aggttcacat tgttaccaga
    16441 ctagataggg acacttctgg cttgatgctc tttgccaagc acggttatgc ccatgcacga
    16501 ttagacaagc agttgcagaa gaaatctatc gagaaacgct actttgcttt ggttaaggga
    16561 gatggacatt tggagccaga aggggaaatt attgctccga ttgcgcgtga tgaagactcc
    16621 attattacca gacgggtggc taaaggtgga aaatatgccc atacttccta caagattgta
    16681 gcttcttatg gaaatatcca cttggtcgac attcggctgc acactggtcg aacccaccaa
    16741 attcgagtcc acttttctca tattggtttt cctttgttgg gcgatgattt gtacggtggt
    16801 agtctggacg acggcatcca acgtcaggcc ctgcattgcc attacctatc cttttatcat
    16861 ccatttttag agcaagattt gcagttagaa agtcccttgc cggatgattt cagcaacctt
    16921 attacccagt tatcaactaa tactctataa aaactgtttc agagtataat tattatctta
    16981 aaggagaaaa ctcatggaag tttttgaaag tctcaaagcc aaccttgttg gtaaaaatgc
    17041 tcgtatcgtt ctccctgaag gggaagagcc acgtattctt caagcgacta aacgtttggt
    17101 aaaagaaaca gaagtgattc ctgttttgtt aggaaatcct gaaaaaatta aaatttatct
    17161 tgaaattgaa gggatcatgg atggttatga ggtcatcgac cctcaacatt atcctcaatt
    17221 tgaagaaatg gtttctgcct tggtggagcg tcgcaagggc aaaatgactg aagaagatgt
    17281 acgcaaggtt ttggttgaag atgtcaacta ctttggtgtg atgttggttt acttgggctt
    17341 ggttgatgga atggtgtcag gagcgattca ctcaacagct tcaacagttc gtccagctct
    17401 acaaatcatc aaaactcgtc caaatgtaac tcgtacttca ggagccttcc tcatggttcg
    17461 tggtacggaa cgttacctat ttggagactg tgccattaac atcaatccag atgcagaagc
    17521 cttggctgaa attgccatca actcagcaat cacagctaag atgtttggca tcgaacctaa
    17581 aattgccatg ttgagctatt ctactaaagg ttcagggttt ggtgaaagcg ttgataaggt
    17641 cgttgaagca actaaaattg ctcacgactt gcgtcctgac cttgaaatcg atggtgagtt
    17701 gcaatttgat gcagcctttg ttcctgaaac tgcagctctg aaagctcctg gaagtacggt
    17761 agctggtcaa gcaaatgtct tcatcttccc aggtatcgag gcaggaaata tcggttacaa
    17821 gatggctgaa cgcctgggtg gctttgcggc tgtaggacct gttttgcaag gtttaaacaa
    17881 gccagttaat gatctttctc gtggatgtaa tgcagatgat gtttacaagt tgaccctcat
    17941 cacagcagct caagcagttc atcaatagtg aaaactataa agcgatatac tatgctatac
    18001 tgtagttatg aaactatgta cgaaaagcac tgccattaat tcctgagaac taaattactg
    18061 attggtgtca aaaaggaaaa cttccaagcg atgatatcct gtctatacac gacctataga
    18121 aatctgtaat atgtatgtcc gtaaaacgat aatttccctt tttgatttta aatgagtatg
    18181 aaaagagaat tttttcatga gaaaacgcat aatattaagt gtcttgacac ctgctattat
    18241 gcgttttctg gctctttgtc aactgtagtg ggttgaagtc agctaagctc gagaaaggac
    18301 aaatttcgtc ctttcttttt tgatgttcag agcgataaaa atccgttttt tgaagttttc
    18361 aaagtttcga aaaccaaagg cattgcgttt gataagtttg atgagattat tggtcgcttc
    18421 caatttggcg tttgaatagg gtagttgaag ggcgttgacg attttctctt tgtcctttag
    18481 aaaggtttta aagacagtct gaaaaagagg atgaacctgc tttagattgt cctcaataag
    18541 cccgaaaaat ttctctggtt ccttattctg gaagtgaaaa agcaagagct gatagagttg
    18601 atagtggtgt ttcaagtctt ccgaataact caaaagcttg tttaaaatct ctttattggt
    18661 taagtgcata cgaaaagtag gacgataaaa tcgcttatca ctcagtttac ggctatcctg
    18721 ttgaatgagt ttccagtagc gcttgatagc cttgtattca tgggattttc gatgaaactg
    18781 attcatgatt tgaacacgca cacgactcat agcacggcta agatgttgta caatgtgaaa
    18841 gcgatcaaga acgattttag cattcgggag tgaaacagtc tgggagactg tttcagcctg
    18901 agcctagaaa tttgaaagcg aagctgttta gccaagccat agtaagggct aaacatatcc
    18961 atagtaatga ttttgacgcg acatcggacg gctctatcgt agcgaagaaa gtgatttcga
    19021 atgatagctt gtgttcttcc ctcaagaaca gtgatgatat tgagcttgtc aaaatcttgc
    19081 gcaataaagc tcatctccat ctcccgattg aaacagtcac tacccggact gtttcaacgt
    19141 cccaagacat aatctcagga agacaagaaa aatcatgttt aaagtgaaaa tcattgagct
    19201 tacgaataac agttgaagtt gagatgaaaa gctgatgggc aatatcagtc atagaaatct
    19261 tttcaattaa cttttgagca atcttttggt tgatgatacg agggatttgg tgatttttct
    19321 tta
//
LOCUS       DAVHIT010000034        18733 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVHIT010000034 DAVHIT010000000
VERSION     DAVHIT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.035"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(79..1176)
                     /locus_tag="VVN16_001066"
     CDS             complement(79..1176)
                     /locus_tag="VVN16_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU5572471.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARESDLPEGIHVLGFTEKGRQHLKYLKGQVSLVSRIGKEPWDV
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
     gene            complement(1186..1932)
                     /locus_tag="VVN16_001067"
     CDS             complement(1186..1932)
                     /locus_tag="VVN16_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5572472.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFSMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2035..2388)
                     /gene="rsfS"
                     /locus_tag="VVN16_001068"
     CDS             complement(2035..2388)
                     /gene="rsfS"
                     /locus_tag="VVN16_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5572473.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2401..2901)
                     /locus_tag="VVN16_001069"
     CDS             complement(2401..2901)
                     /locus_tag="VVN16_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU5572474.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            complement(2902..3495)
                     /gene="yqeK"
                     /locus_tag="VVN16_001070"
     CDS             complement(2902..3495)
                     /gene="yqeK"
                     /locus_tag="VVN16_001070"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU5572475.1"
                     /translation="MAYQDYINCSREVLLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRHQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(3495..4124)
                     /locus_tag="VVN16_001071"
     CDS             complement(3495..4124)
                     /locus_tag="VVN16_001071"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU5572476.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4176..4487)
                     /gene="yhbY"
                     /locus_tag="VVN16_001072"
     CDS             complement(4176..4487)
                     /gene="yhbY"
                     /locus_tag="VVN16_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU5572477.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(4702..5808)
                     /gene="yqeH"
                     /locus_tag="VVN16_001073"
     CDS             complement(4702..5808)
                     /gene="yqeH"
                     /locus_tag="VVN16_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU5572478.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSNALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(5811..6401)
                     /locus_tag="VVN16_001074"
     CDS             complement(5811..6401)
                     /locus_tag="VVN16_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU5572479.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(6353..7261)
                     /locus_tag="VVN16_001075"
     CDS             complement(6353..7261)
                     /locus_tag="VVN16_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5572480.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7318..7878)
                     /locus_tag="VVN16_001076"
     CDS             complement(7318..7878)
                     /locus_tag="VVN16_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU5572481.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8017..9224
                     /gene="sstT"
                     /locus_tag="VVN16_001077"
                     /pseudo
     CDS             8017..9224
                     /gene="sstT"
                     /locus_tag="VVN16_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9292..10245
                     /locus_tag="VVN16_001078"
     CDS             9292..10245
                     /locus_tag="VVN16_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU5572482.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREKKIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            10388..10768
                     /locus_tag="VVN16_001079"
     CDS             10388..10768
                     /locus_tag="VVN16_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5572483.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            10867..11088
                     /locus_tag="VVN16_001080"
     CDS             10867..11088
                     /locus_tag="VVN16_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU5572484.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            11105..11419
                     /gene="trxA"
                     /locus_tag="VVN16_001081"
     CDS             11105..11419
                     /gene="trxA"
                     /locus_tag="VVN16_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5572485.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            11724..12257
                     /gene="queF"
                     /locus_tag="VVN16_001082"
     CDS             11724..12257
                     /gene="queF"
                     /locus_tag="VVN16_001082"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU5572486.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
     gene            12307..12531
                     /locus_tag="VVN16_001083"
     CDS             12307..12531
                     /locus_tag="VVN16_001083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572487.1"
                     /translation="MLKAVFEVADRTDEVSLKHCFEVADRTDEVSLKHGFEVVDRTDV
                     VSNHTYGKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(12556..13224)
                     /locus_tag="VVN16_001084"
     CDS             complement(12556..13224)
                     /locus_tag="VVN16_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU5572488.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEK"
     gene            complement(13335..14125)
                     /locus_tag="VVN16_001085"
                     /pseudo
     CDS             complement(13335..14125)
                     /locus_tag="VVN16_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
     gene            complement(14371..16167)
                     /gene="pepF"
                     /locus_tag="VVN16_001086"
     CDS             complement(14371..16167)
                     /gene="pepF"
                     /locus_tag="VVN16_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU5572489.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNDLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(16178..16921)
                     /locus_tag="VVN16_001087"
     CDS             complement(16178..16921)
                     /locus_tag="VVN16_001087"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU5572490.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(16923..17873)
                     /locus_tag="VVN16_001088"
     CDS             complement(16923..17873)
                     /locus_tag="VVN16_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5572491.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNMD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(17886..>18733)
                     /locus_tag="VVN16_001089"
     CDS             complement(17886..>18733)
                     /locus_tag="VVN16_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5572492.1"
                     /translation="ENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDGYIWNQIAEQSIRSS
                     VPVIIYHYVPHFDAGNPTRVKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIF
                     NLMER"
BASE COUNT         5279 a         4299 c         3610 g         5545 t
ORIGIN      
        1 tttatcattt tccaaaaact ttatcttcaa tttgttagct aaaatttcta caataaaaaa
       61 gcctaccttt cagtagactt agtttgtttc tattctaatc ggcactctgc caaaattctg
      121 ttctgctata cttggatttc ccaattggta aatctggtct gccttttgag tcatgacatc
      181 ccaaggttcc ttgccaattc tgctgactag actgacctgc cctttcagat atttaagatg
      241 ttgcctgcct ttttcggtaa agccaaggac atgaatccct tctggcaagt cactttctct
      301 agcctgcacc aagatatagg tcaagaggcg tctgacacgc gccttggtgt aacgtttggt
      361 ggtcactaac tcgaccaatt cttccacaga ctgggctgtt ttaatagctt ctttaatgcg
      421 cacagccatt tcttgattga cctgatagat ggtggttagg tcgggatttg acaagatttg
      481 atagcggagc aagggaaaat agtcttccca gatcacctta ctggcctgct caaatagggc
      541 aacagaaggc ataaaacgtt ctaagaaatc ttggtccttc tgatgttgac ggagggctgt
      601 cgccgaggca aagtccacat ctttattcac agaatggtaa cctgccccct gacgctgaat
      661 cggatgcagc ttgatatttc gtcctgcaac cgcctttgca taggccagag caagaacatg
      721 attgggggta ttacctgaaa aatcaagacc agcaaattcc ttccacatag cttgggtttt
      781 ctggggatag gaaagggaat caggcagatt ttccacaaat ttctccatct cagcaccttt
      841 ctctgtgtat aagtcagcga ttttctgata atctcgaact tcctctgtcc caaaaactag
      901 actatcaatg cccaagcgat ccaagatatc cacagctcct tgaccaaaga aatcagctgc
      961 ctgaacactg actaaaaagg gcaattccac taccaagtct gcgccatttt ccagcgccat
     1021 ctgggccctt gtccacttat ccacaatagc aggctctcca cgctgcatga aattccctga
     1081 catggcaaca attttcagtc cctctgcctg atccagcagg tatttatggc cattatgaaa
     1141 aggattgaac tccgcgataa tacctgtgat ggtcatgcta atctcctact tctgcgccac
     1201 aaaaaaccaa cgggtgctag tttctgttgg ctccttatcc tcaaagtctg catagagttt
     1261 gaaggacttg aatccagcct gttccagcaa gatatcatag gtcaagatct cataggtcct
     1321 ctcctcatgt acttcatcgt ggcgactaaa ggaaccgtca gcctccttga taaagaaagt
     1381 cagctcatgc acgatggagt gaggaacttc gtcctcatat gtatcccaaa gcatggaaaa
     1441 atcttccgca ttttcatggt aggaatagcc agggaatact tcatctgtct ggtaggtcga
     1501 gtgcacgtca aagatgaaaa ctccctcttc attcagcgca ttgtacacgt ccttaaagac
     1561 gtcccctact tccacctcat cctgcatata gcagatagag tccgaataac acgtgacaaa
     1621 atcgtatttc cctgccttgg acaaatttag catattacct tctataaaag caatcttttg
     1681 cttggctgaa gttgctctct tctccgcaat cttcaacata tccgcactca agtcaagtcc
     1741 agtcacatca aaaccagcct gagagaagcg cactgattga attcctgtcc cacaagccaa
     1801 ttccaagagt ttctttctct ccttggtctt aggcaaatga cgcagagaaa aattcgtcca
     1861 tttgtcgtat aaactatcgt ccatgaccgc atcatagaca gccgcaaagg tttcataagt
     1921 cgccataatc atgatactaa gagctccatg gtaaacacca ctagcccttt acctttctat
     1981 caatttttct aaaataagca aagaaaccca gttgactaca actgagttca tcttctaagc
     2041 aagagcttct gaaatatcta ctgaattcgc ctcatgccat agcttctcta gattataatg
     2101 ggcacgcatt tcttctgaaa agatatgcac gacaacagca ccgaggtcca gtaagaccca
     2161 gcctccagct gcatcgcctt cgacatggct acctttaaag cctgcttgag ctactttttc
     2221 acggatatta gctgcgatag cgtccaactg acggctattc attgagctag tgatgacaaa
     2281 gtagtccgtc acactagtca aatcttgtac atcaagtgcg aggatatcct ccgcacgttt
     2341 ctcatcagcc gctttcacga ctagttctag taattctttt tcgttcattt aatcctcttt
     2401 ttagaaaata tgtttgtaat ctaggacatc tgcaaagtgc tcctcccaaa tgtcctcata
     2461 aaattcatta atcgtttctg ctggaatgtc atccccatca aaggttgtga cagcaccttc
     2521 tggaacaata agctgatagc catattcaaa ggcaaccttg acagaggtat ccacacaata
     2581 ttctgtctgc ataccacata aaactaattt ttcaatcccc tgtttatcca agtattcttt
     2641 taagccagtt tctttgaaaa tactgttata cttcttctga aagacctttt cagcaggttt
     2701 tcgatttaaa agcgcagata actgccaatc ttctgatgtt tgagcttcag agttctcaat
     2761 atgttgaaca tagataattt caatattctt gcttctagcc tggttttgta aataagaaat
     2821 ttttcccaat agactttttg tctggaaccc agtttctact aaaacattct gaacatcaat
     2881 gattataaaa gccgtcttca tttagttctc tttcaaatag tgcacaaagg cgttataggt
     2941 ttcaagggtt tggggataga tggggaatcc ctgatgagct agatactcca cggtacgagc
     3001 tgtttcgtag gccaccgcct tattgagcga tagacttgca atctcacgcg ccacatctac
     3061 tccaggaaag gctcgattgt gctcgatata gtctgcgacg tagatgacct tatctaggtc
     3121 tgtcatttga ccagcaccga ctgtatggac ttcaatggct cgcagaattt cagaatcatg
     3181 caaatccaaa tcttcctgaa ttttgtaaat tccgaccata ccatgccaga cattattgcc
     3241 ccagtttttg aggtcagggt ctagctggtg acggtcaatc aaggctagaa attcctgatc
     3301 tgacaacttt ttagcatagt catgaagaag acctgctaga cttgctttct caacatctac
     3361 accaaagcgc tgagctaatt ccatagcggc acgctccaca cctagacaat gggttagacg
     3421 tttctcaggt agaagctctg ccattttttc caacaaaacc tcacgagagc agttgatata
     3481 gtcttgatag gccatcagta gagcccttcc ttctcgatat agtctagcac aggctgaggt
     3541 aggagaaagt tgggtttccg accttgggca atgaagtccc gcaccatgct ggacgagata
     3601 tccatgagcg gtacgtccac ccagataact ggataggaag tccctgcctt gtagcgtgga
     3661 cgctgaaccc ccacaaactg aaccatgtca accagttcat caattcggta ccacttaggc
     3721 agatagtcaa ccatgtcggc gccgatgata aagtaataat ccgtatctgg atgttgctct
     3781 gtcaagattt tcatggtgtc gtaggtgtag gaaataccct tgcgctccaa ctcaatggtt
     3841 tcaatgacta ggccgtcaat cccctcaatt gccaactcaa gcatcttgag acgatggtgt
     3901 tcagggatgg tttccttttt atcaacgtga ggaggttggt attcaggcat gagcagaact
     3961 tgatccagtc ccaactgttg ccgtacttga tccgcaacaa tgagatgggc attgtgaaca
     4021 gggttaaaat tcccccctaa aatcccaact tgtttgcgtt ttttctcctt gatttctggc
     4081 tccaactcta ccttggtaaa gggagtcaat agttcgattg tcataggcta gtccccttta
     4141 tttctctata aaaacagtta tttggagttt cggtcttaga tttctttgac tttcttagaa
     4201 atcttgcgat tttctttctt gctagattgt ttaaacaaaa tcaagatgcg tcctattttt
     4261 tggactgtat ccacaccgat ttcttcttcc aaaatttcag ctacttcgtg gatgttttca
     4321 tctgtgtttt gtaagagagt aaccttgatt aattcacgcg catcaagagc ttgacggacg
     4381 ctggttttga tttggtcgtt gagtccattt ttcccgattt ggatgatagg tttgagggtg
     4441 tgtgcctggc tgttgaggaa ggcacgttgt tttgatgtta atgacataat ttctttaaaa
     4501 gagtttcttt ttatactttt ctgaagtggt gacggacgtc agcaaagtcc ttcggacttt
     4561 catgactaaa atttgagcct aaggtctcaa attttccgca gttggtacaa tcacttgtac
     4621 caacttacac cacagcgaaa agtattctct atggggctcg ccttgctcgc ccaaattcag
     4681 acaacccttt cctttctgtg cttaaataat tgcttttcgt gtgacgacgg cgacgccttc
     4741 tggtgcccag acggcgactt ttgctatgcc tgttacacga atccagccta ggcctgagat
     4801 gactaggtct gtcttatctt taatggtaaa gacatgctgg actagccttg ggaaatcttc
     4861 tttttccttg ctatttggtg gtgtcagaag agttcccagg tgcttatcgt agaaagcact
     4921 agctccttca agcttgctac gatggagttt gagttcatta tcaaagaaag cagtaaatcc
     4981 ttgcttttct cctgctataa agtcaaagcg tcccaaacca cctaaaaata gggtttgctc
     5041 aggattaagc tgataggtct taggcttgat ttccttttta ggactgacat acttgaggtt
     5101 tttggccgtc aagtagtgag ccatctggtg gcggtggata attcccggcg tatcgtaaat
     5161 ataagatccg tcgtcaagcg gaatctctat tttgtccaag gttgtccctg ggaagcgtga
     5221 agtagtgatg acattctgat cacccgtgat ttcttggata atagcattga ttagagttga
     5281 ttttccaacg ttggtcacac cgaccacata gacatcgcgg cccttacggt agtgttcaat
     5341 cttgtcaatg acttccttaa tggcatgttt attttgtgct gaagttagga ccacatcgac
     5401 tggacgaaga ccttcttcat gggcacgttt catgagccac tggctaatct taccagactt
     5461 aactgactta ggaaggatat cttttttatt tcctaccaag aggacatcat tgcccgagac
     5521 gaaacgtggt aaacctggga tgacagatcc attaaaatca aagatatcaa tgacattgac
     5581 cactaaggca ttactgtctc ccacctcgtg caagagcttg aggaaatcat cgttcgtcaa
     5641 ctggacatct gtgatttcat tgtagtggcg gagacggaaa cagcgttggc aatagacttc
     5701 gccagtctcc aaaccttttt caagtgccga ctggggggta aaaccaagac cagctttatc
     5761 tgtcgtctga atggttgctc cacaaccaat acagagaatt tcttccatag ttaaattcct
     5821 tttttatatg taatcggtcc gtacttttca gtgatttttc tcataacacg acgctcacga
     5881 gttcggttaa tctgcgtttt gattgagtca tgttggacca agggtttgac taaaattgac
     5941 cgaatccctg cacggtgggc tgctcgtata tctgtcatga gctggtcacc aaccatgacc
     6001 acttcctttt tgtcatagtg gaattccttc atagcacggt caataccaaa tgtgaagggc
     6061 ttcaaggccc agtaaacgta atcaatccca aatttctcaa ctgctcgttg aacgcgtttt
     6121 ttggtgttat ttgacactac gataatgcca atacccgcgt cccgaaggtc atgtagccat
     6181 tgcttcatct ctggcgttcc atcagggttg ttccaagcaa tgagggtatt atccaaatcg
     6241 accaaaacag ccttgattcc ctgcgcctgc aggcttggga ctgtcagatc atagactgct
     6301 tccacagcaa aatctggtat ataattttca atcgccattc tggctccttt tcttaacttt
     6361 ttttcgcaat tttccttagt aacaaggata aaacaatcca caaacctgca ctagccaaac
     6421 tgatatagag ccaagcatgg ggctcatctg ttaaaggcaa gggaacattc attccgaaaa
     6481 agcctgtcac gactgccaaa acagctagca agactgaaat gatagtcaag gttgtcaaat
     6541 tgtcattcag attattgttt agaatattgt tgtaagaggc tgaaagctgc tgtaaaatct
     6601 gagagattag gtctgtcatg gataccagct gatgagcctc aatcatggca tcatcaaact
     6661 gttctctctc aatctcatca aaactacgat acaaggcatg accttgaata tgctctaaca
     6721 aaatccgatt ttgtttggca gctgccgtta gataaaccat accagtctcc aaatcagaca
     6781 gggcaaagag gtttttcttg gtagttcgct ggcgcaagag gtcattgacc tcatccctac
     6841 tcttgtccat ctgctcaatg acaggatagt aggcattgct gatgatttcc agactggcaa
     6901 agagaaactt ataaatcgaa agcgtgtcat ggctctccag ataacgagtc atctgttcaa
     6961 tgacataggc gttcttggta ttactaatgg taatcaggcg acgatgctcg acaataaagg
     7021 tcatgggaaa agtctcatag taggccttgt ccttttttac gtctaggaca ttatagataa
     7081 aggtaaccgt cccactttca cggtggtagt ccatgtgggc gcgctcgttt ctatccagtg
     7141 cgtattcaat ggtttccttg tccaaaccgt aaatttcaga aaggtcttct agtttattca
     7201 actttcctag gtctaggtct atccaggtac aaccattgcc caactgcttt tctaaaacca
     7261 tcttttctcc ttattaaact ccctctattg taccacaaat tacttaaaat aacaggtcta
     7321 gtctgtgcgt gagaaattgc taacaactgt gatatttcgg ttagggacaa agtgaagggc
     7381 ttgctcctcg ctacgaagct gtgtcgtacg aattcccaca ctgacaacct tacccgatac
     7441 agtaatcggt ccatttgtca gaacgacctc atctcccaca tccagttgac gttcaaagag
     7501 gatgaaaaag ccattgatga catcagacag aaagccttgg gctcccatac caatcgctac
     7561 cccagcaata ccagctccag ccagcaaact agaaactggc aaacctaaaa tcgacaaaat
     7621 gcagtagagt aaaaagaaat atagcgtata attaaacaca ttttctagta aacgtgagat
     7681 ggttttttgg cgtccgacat catgacgaga catttttaga gaaggtttga caattctctg
     7741 caccatggta tgaagcattt ttttagcaat ataaaataca atcaaaagca gtaaaagaga
     7801 aatgaccttg gttagaatat tctcgataat ggttgtcaca tctagctttt caatataagc
     7861 ctgaataaat ttttgcatat aaaactcctt tatttcatta taccatattt tcatcagtct
     7921 cattctccat aaatattcaa aaaatagaca gaaaattctt gattttgtct atcatttata
     7981 ctataatcat aatcactaca caaaaaggag attattatga aaagaatcat tcatgcttgg
     8041 aataaggcaa gccttgtcaa gcgtatcttg attggtatgc tttgtgggga atcctaggac
     8101 tgacccttcc taatctctca gggattgggc tactcgggga tttattcgtt ggaggtctaa
     8161 aagctgttgc tcctatccta gtctttgccc tcgttgccaa tgccctttcc aacatcaaaa
     8221 gggacaagat agcaatatga aaactgttgt tttcttgtat atcctatgat agggactttc
     8281 gccgctgctc ttgtagctgt actagcaagt ttcatcgtcc ctattgaaat taccctaaat
     8341 agtgccaata ctgaaattgc accaccagat ggggttgggc aggttctcag caacctcttg
     8401 ctcaaactgg ttgacagccc agtcaacgcc ctgtttactg ctaactatat tagaatctta
     8461 tcttgggcag tcatttttgg aatcgctatg agagaagcca gtaaaaatag taaagaattg
     8521 ctaaaaacta tcgctgacgt gacttctaaa attgtcgaat ggatcatcaa tctggctcca
     8581 tttggaagcc ttggtcttgt ttttaaaacc atttctgaca agggagtcgg aagccttacc
     8641 aactacggta ttttattggt tctattagta acgactatgc tttttgttgc ccttgtggtc
     8701 accctttgat tgccttcttc tttatgagac gcaatcctta ccctctagtt tggaactgcc
     8761 tccgtgtcag cggtgtgaca gcctttttca ctcgtagttc tgcggctaac attcctgtca
     8821 acatgaaact ctgccatgac cttggactca acccagatac ctattctgtt tctatcccac
     8881 tcggttctac tatcaatatg gctggagtag cgattaccat taaccttttg acccttgctg
     8941 cagttaacac tcttggaatt cctgttgact ttgccacagc ctttgtcctc agtgtggtag
     9001 cagctatctc agcctgtggt gcttcaggta ttgccagagg ttccctcctt cttatcccag
     9061 ttgcttgtag ccttttcggt atttctaacg atattgccat acaagttgtt ggggttggtt
     9121 ttgtgattgg tgtcatctaa gactcatgtg aaacagccct taactcttct acagatgtcc
     9181 tctttaccgc cgttgccgaa tacgcagcaa cccgtaaaaa ataactcatc aaggcaagcc
     9241 tgctcatgtc ttgtctttta cgcttttatt ctaacttatt aggaaattct tatgtctatt
     9301 agccaacgta cgaccaagct catcttagct acctgtcttg cctgcctgct tgcttatttt
     9361 ctcaatcttt cgtcagcagt ttcggctgga attatcgctc tcttgagcct atctgatacg
     9421 cgtagaagta ctttaaaact ggctcgcaat cgtctttttt ctatgcttct agctctggct
     9481 atcggtgttc tagcttttca tttgagcgga tttcatatct ggagtctcgg cctctatctg
     9541 gccttctacg ttcctttagc ctacaagatg ggctgggaaa ttggtatcac accaagcact
     9601 gttttggtta gccatctctt ggttcaagag tcaacctctc cagaccttct agtcaatgaa
     9661 ttccttctct ttgctatcgg tacaggattt gctttactgg tcaatctcta catgccttca
     9721 cgagaaaaga aaatccagca ctaccacacg ctggtggaag aaaagttaaa agatatcctc
     9781 cagcgcttca aatactattt atccagagga gacggacgca accgagcaca gctggtagca
     9841 gaattagaca cgcttttgaa agaagccctc agactggtct atttggatca ctctgaccac
     9901 ctctttcacc agaccgacta ccatatccac tactttgaga tgagacaacg acaaagtcgt
     9961 atcctgagaa acatggccca acagattaac acttgtcacc ttgccgccag tgaaagcctg
    10021 atcttagcgc aactcttttc aaaaattgca ggtcaactga gccagaccaa tcctgcttct
    10081 gatttgctag atgaaattga acgttatctg gaagtcttcc ggaaccgcag tctgcccaag
    10141 acaagagaag agtttgaaac ccgcgccacc cttcttcaac tcctacgtga agccaaaacc
    10201 ttcatccaag taaaagttga tttttaccaa aaatatagac agtaaaaaat aaaaccacta
    10261 aaaaacagag cttgccagca ttcttgaaaa gtagtataat tattgcatgc gcaatcatct
    10321 tgtgacgcag agactgcccg ttaatggact ttcttctatt tatgactcta aaaagaaagg
    10381 agacactatg acctatttgg aaaaatggtt tgacttcaat cggcgtcaga aagaaattga
    10441 aagtctcttg gaagagacca ttgcccaaca gagcgagcaa agtctgacct tgaaagagtt
    10501 ctacctgctc tactatctgg acttggctga agaaaaatct ctacgccaga ttgacctgcc
    10561 agataaactt catctgagcc cgagcgcagt ttctaggatg gtggctcgct tggaagctaa
    10621 aaattgcggt ctactcagtc gcatgtgttg ccatcaagat agacgctcta gttttatctg
    10681 cctgacaaat gatgggcaaa agacactggc ctcgctacaa aagactgtcg aagaaagctt
    10741 ggaagcaggt ttggatttct tgatttaaga tgattttaaa aaaagttgcg tgcgcaatca
    10801 tttttcttga cattctcttt tataaggagt aaaataaagt tagcattaaa caaaggagtt
    10861 ttaaatatga ttgaaattac ctatatagat gccagcaaga acgaaagaac tgtgactttc
    10921 gagtcttatg aagactttga acgttcgcaa caagcttgcc ttatcggcgt cgcagactac
    10981 taccctgtcc aaaaattaac ttacaagggt cataatttgg actaccatgg gacttatgga
    11041 gatatcttct tctatctcat gaaacaagat ttaagccaat ataactaaaa aaaggagaaa
    11101 tacaatggca aaagcaatta cagatgcaac attcgaacaa gaaacaaaag atggtttggt
    11161 cttagtagac ttctgggcaa cttggtgtgg tccatgtcgt atgcaaggtc caatcttgga
    11221 caaattgtct gaagaacttt cagaagatgt cttgaaaatc gttaaaatgg acgttgatga
    11281 aaatccaaac acagctcgtg cttttggaat catgtctatt ccaactcttc tcttcaaaaa
    11341 agacggccaa gttgtcaaac aagttgcagg tgttcacaca gcagaacaaa tcaaggccat
    11401 cattgctgaa ttgagctaat caaacgagag accaagtact ttctttggtc tcttttttct
    11461 tgccctttgc catttttcaa aaaatatgct agactatagt agattgaaac tagaatagta
    11521 cacctctact tctaaaacat tgttagaaat cgatttaact gtcctgatcg atttgtcctg
    11581 ttcttatttc attttactat ataagtagaa tattacgatg tttgggatat gccatcgcta
    11641 aaaaaactcg atttggtatt ttttagctcc ctaataggag ctttttgcat gctctgaaca
    11701 tttcccattt tggaaggagt actatgaaac gtcaatcagc cttggtcgtc tttagtggtg
    11761 gtcaagattc aacaacctgc cttttctggg ttatgcaaca ctatgaaaca gtcgaagctg
    11821 tcacctttgc ctacggccaa cgtcatcacc tcgaaattca aattgctaga gaaatcgcta
    11881 aggaacaggg cattcgtcac catatcctcg atatgtctct gctgggacaa atcactgctc
    11941 agccagactt tgcgacgatt catatttcct acattcctga caagctctgt gtcgagtcaa
    12001 aatccctcaa actatatcta tttagctacc gaaatcacgg agatttccac gaaaactgta
    12061 tcaacaccat cgggaaagac ttggtcaact tgctagaccc tcgctattta gaagtctggg
    12121 gaaaattcac tccgcgcggt ggcatttcaa tcgaccccta ctacaactac ggtaagcaag
    12181 gaactaagta tgaggacttg gcagaacaac gcctcttcca acacgacctt tatccagaga
    12241 aaattgacaa ccgctaaagt catactcaat gaaaatcaaa gagcaaacta ggaagttagc
    12301 cgcaggttgc tcaaagcagt gtttgaggtt gcagatagaa ctgacgaagt cagcttaaaa
    12361 cactgttttg aggttgcaga tagaactgac gaagtcagct taaaacacgg ttttgaggtt
    12421 gtggatagaa ctgacgtagt cagtaaccat acctacggca aggtaaagct gacgtggttt
    12481 gaagagattt tcgaagagta tcatacgaaa aagccctgtt cctcaagatg aggagcaagg
    12541 ctttttgagt ttcaattatt tttctgttcc aaggaaattt tttgcaacaa gggctgcaag
    12601 aactccacca gcgattggtg caaggatgaa aatccaaact tgttgaaggg ctgcgccgcc
    12661 taccaagaca gctggtgcca agctacgagc tgggtttact gaaagtccag taatcttcaa
    12721 tccgacaaga atcatcgcca tcaatgacaa accgattacc aaaccagcaa tcgcgccatt
    12781 gcccttactt tctgaagtca cagtcataat aactaagaca aacaagaaag ttgcaatgac
    12841 ttcaaacaag aaaccaccaa agacagtgac accgtttgcc aaggcatttt caccaagact
    12901 agcagttgac atacctgaat tagccaagag gaagaagact gcgccagaag cgatgaaagc
    12961 tccaacaacc tgaccaagga tgtagtttac aagttctgaa gatgacaaac gtttgtttac
    13021 aaacatagca atcgaaacag ccgggttcaa gtgagcacct gaaacagttc cgattgagta
    13081 ggctgccacc acgattgcca aaccaaaggc aaaggcgatt ccaaggtgac caaggccatc
    13141 aagaccattt ccaaaaacaa cagctcctgt cccaacgaac acaagcatga acgtaccgat
    13201 taactcagca acaaattttt tcattttctt tctccttttt tcaaaaacta gatactagtc
    13261 tatcaaaagt aggaaagggt ttcaagaaaa ttgattggaa attttttgaa aatcatagaa
    13321 ctattagcta atctctagta ttgaaaagac tggatagctt ctttcaactc atcttgtaaa
    13381 ctatttctct ggtcaagttg gacatagact tccaccagac aggatctaaa gttggaaaat
    13441 ttgtaaaaat cctccctttc ttctatcgga aaatcaacag tttttatcca agaagctact
    13501 tgttcttgct ccaacttccc ttataaaata ggttcataga tcactcttgc taaacgccaa
    13561 tcctcatcat ctgtaaagcg aatcgacatc cttttaaata gttggccaag tatatcaaat
    13621 acttcatgaa ctctgttttt aggaaagtct ggatgacaaa ccacctctgt cagtaaatcg
    13681 gctccatgtg caaaagcgtg aacccaacca tactgacttg agaaacctgt tgtatccttt
    13741 tcttttgaaa gatagtgcaa gccttgattt gaaaggtcat tacgaaatcc tgcatttaat
    13801 tcctgataaa aaaccgattg ctggttggca tcgtcagaca agagagttgc ataaataagc
    13861 gccctaaaga acgttcaagt gttggcaggc ctatcttatc aatctcttta tctaaccctc
    13921 catcagatga aaccccttca gcaatgaaat gaaattgctc ctgtgtaaat agctcttcct
    13981 gaatccctct agcaaagctt gtaaaaacaa ggtcatcacg aatttctgga gaaggatctc
    14041 ccaaatgatc aagcaaccac tggatttcct cctggttata atttggtttt tcttctgcta
    14101 tttttcttag taaatcttga tacatggtca atacctctac atttctagca actgttcaaa
    14161 aaggcagtct taaatgactc aatattgaat tctcaattaa atacaatctg atataaaatg
    14221 acgtaaataa ctatcaatac cagttctaca gtaagttcaa atttaacatc acgaccttca
    14281 acgacatttt tgaaaatagc tacaactaag acaaatagaa tgacgcttaa caagcccata
    14341 aacatcattc taaaaaattt ttctattccc ctactctccc aactcagcac tataggagat
    14401 aatctggtca actgtgtcag acaagaattg gatggtatca cggagtggtt tgtctgttga
    14461 aatatcagca ccgataatca tggctgactc aagtggtgtc ttgctaccac ctgatttgag
    14521 gagattgagc cagtcttcag ctccagtttc agaatgtttt agatgaaggt aaccagcagt
    14581 cgagataact agtcctgctg agtaagtgta actatacaag cccatatagt agtgagcttg
    14641 gcgcatccaa gtcagagttg catcgtcgtc gatttcaatg gcatcgcccc aaaaatctgt
    14701 caaaacttcc ttcataatgc tgttgagctt gcttgctcca aaggtctccc cttcttcaat
    14761 caatgtatac accttacgct ggaaggcggc ttccaagagg tgggtgatga aattatggaa
    14821 gtaggtgtct gtcaagcgat gagccagagc gaagcgtttt tgacgtgggt cattagactg
    14881 gttctctaag taatcactga gtagcaattc attgaaggtt gacggtgctt caacataata
    14941 ggtcgacatg tgggcgttga aatagctttg gtgattgtct gaaaagatga attgaccaga
    15001 atgtccgatt tcatgaatca aggtatagac atcgctcaaa cggcctgtcc agctcatgag
    15061 tacataaggg tgtacgcgat atgggtccgc cgcataacca ccggaatcct tgccactgtt
    15121 agcagcaaag tccacccagc gctcttcttg gtaacgagca acttcctgac aatattcttg
    15181 ccccaaaggt tctaccgact tcatgaccaa atcataggca tcgtcaatag tcacttcagg
    15241 attcagggcg ctgtccaagt ccaatttcca gtctgcaaag gtcatctttt caagatcatt
    15301 taccttggca acatgcttga ggtatctctg agcgactggt gcaaagtcct tcatgataag
    15361 gtcaatctgg cggtcaaaca tgacacggtc cacttcttgt tcagctagaa gatagtcaaa
    15421 gacagagtca tatcccttca tatcagccaa gagtttttca gacttgacct gagccagata
    15481 ggctgctgca gccgtatttt ggtgcttacg aagtccctct gagaaggaac ggaaggattt
    15541 ctcacgaacc tcagcatcct cgtgattttg gtagaaattc tcataggtga caaagctgtt
    15601 tttgtaggtc ttgccatggg cttcaaagtc agccatttca aaatccccag ctcgcatctt
    15661 agtataaatg tcctgcggac tgtagaaaac ttcaccgaga ttggtcaagg ccttctccac
    15721 atcagcccct agatagtggg cttttttgat tttggcctga cgaatagctg ccgtcaagtg
    15781 aggcaattca cccaaacggt ccaagacttc ctcgtctgct gccaccaagg catcgtcaaa
    15841 gaaggtcaag gctacgctgg catctgtttc aaattccatc ccagcttggg caatattggc
    15901 aaattcgtca ttgctatagt ccgtcgtctg aggcataaaa gcatagttgc caatatggct
    15961 catctgaatg tagatctgtt ccaattccgc aaaggccttc tcgaaatcct caaaagtgtg
    16021 aagattgccc ttgtaatcac ggctaaactg gttgatgtct tcgcgagctt tctcgattgc
    16081 acgcaagaaa tcctcacggt cttggtatag ggctgttaag tcccagagtt ccttctctgg
    16141 aaattctgaa cggtgttttt gttccatttt cttcctctta tttctctaat tctactaaaa
    16201 cactaagggc tgataaggcg taaagcggtg ctgtttctgc tcgcaaaata cgaggaccta
    16261 ggcctaccaa aacggctccc ttagcttcaa aactttcgat ttctgcaggt gacagaccgc
    16321 cttctggacc aaagataaag agcagtttgg ctcctgtttc aagaccagtg actgcttgca
    16381 gaagcgcagc ggcttctcct tctttagctg attcttcata ggctactatg atagagtcaa
    16441 actggtccag ctgagctaga aaatctgctt ttttctcgaa aagtttaata cttggtacaa
    16501 tattacgctt gctttgctcg gctgctccaa gggcaatttt ttctagtttt tcaacttttt
    16561 tacccaattt cttgccatcc cacttggcaa ctgaccagtc tgcaggaaag gcccagattt
    16621 ggctagcacc cagttcggtt actttttgag cgatgaactc cagcttgtct cccttgggaa
    16681 atccagatgc gatggtcact tggactggta gttccacatt gtcatttaat tcttggacca
    16741 actcaaactg acgattttcc atatccagca cgcgcgccaa gcgcttgatg ccatcatcaa
    16801 agactaaggt aacctcatcc tcttctttca agcgcataac ctgaaacata tgcttactgg
    16861 tttccttgtc ctcgatagtg acaggagaga tagcactgcc ttttacaaaa tactgctgca
    16921 tgctagcctc caatcacacc agagatatcc ttggttttct taaagacaca ggtattccat
    16981 tccccttgaa ccatgtgagt ttcgaggaaa aatccagctg attcagccga ctcgcgcacc
    17041 atgtcccact tatccttgat aatgccactc atgatgaggt agccttcgtc cttgaccaaa
    17101 cgataggcat cgtctattag atgaatgagg atatccgcca agatattagc cacaatcaca
    17161 tctgcctcaa tttccacacc cttaagcaaa tctccagccg ctacatggat attttccatg
    17221 ccagggttga gctcaatatt ttcctgagcc acacgaaccg ccacatcatc caggtcatag
    17281 gcgaaaattt ctttagcccc cagaagcgag ctggcaatag agagaacccc tgaaccagtc
    17341 cccacatcta gcaccgtttc gccaccacga agaacctgtt ccaaggcaaa aaggctcatc
    17401 ttggtagttg ggtgggttcc agtaccaaaa gccatgccag gatccagctt gataatcatt
    17461 tcccccgcag tcgcctcata gtctgtccaa gagggaacga tggtcaaatc atgagtgata
    17521 cgagctggct catagtattt cttccagttg tctgcccagt cttcctcagc caaggcagtc
    17581 gtacctattt ttaactctcc caaatccata aaatccgtca attctgctaa gcgagcctgc
    17641 aagtccgcct caaccactgt cacatccacc gtgtcagggt agtaggctgt cactacgatt
    17701 tcttcttgct gctccacctc tgggaaaatc tcgccaaagc ggtccatatt tcccacatag
    17761 tccatgctgt cttcaatcgc aacaccttgc gctcctagct caatcaagag attggaaacc
    17821 aactcctctc cctcacgctt cactgtaact tttaactctt gccatgtttc cattatgtat
    17881 tttgcttacc tttccatcag attaaaaata gtctttccaa taacaacaat aaggttatct
    17941 cttaatttct tctctaactc taaatcaatt ccactatttt gtatgaattt gtcttctaca
    18001 tttctatata agcgttttac tctagtgggg tttcctgcat caaaatgagg tacataatgg
    18061 taaattatga caggaacaga acttctgatt gattgttcag caatctgatt ccagatatac
    18121 ccatcagtct ctcccaaaga aaccccataa agaattatga tgtcagaatt ttttataata
    18181 tcgctatttt taacatccat attttctcta tagttcttca aaactaaatt ttttattaag
    18241 gattcttttt gttcttcatc aaaactattt gaaatctgtg tctcgtcact aactcctaaa
    18301 actgtgcaaa tatctaccgt tccatgtgca tgaattggag gcgcaatgtg aaatttataa
    18361 ttattagttc gtaattgatt gctaaatgat tttgctgact cattatataa tttatcgatt
    18421 acatttgtgt aattaagagt taatatattc acgatatcgt gttgatatag attttcatta
    18481 tattttttat cgatagcagg ttgattaatt acagggagag aggtcctcat attatctaga
    18541 gtgcttataa agtcaattgc attacctttc tcaagatttt tttcttgttg tatgcgtaga
    18601 tattctctta aatcatcaac tacttcacta aaatcatcta taaatttctc ctttatttct
    18661 atacttgagg agatgaggtc gttatctttt gttaatttac caattgacaa ttcaaaatct
    18721 gaccaatttt cat
//
LOCUS       DAVHIT010000035        18710 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVHIT010000035 DAVHIT010000000
VERSION     DAVHIT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.036"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(317..1123)
                     /gene="yidA"
                     /locus_tag="VVN16_001090"
     CDS             complement(317..1123)
                     /gene="yidA"
                     /locus_tag="VVN16_001090"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU5572493.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKSNDESGVAHAIRTWVL"
     gene            complement(1136..2440)
                     /locus_tag="VVN16_001091"
     CDS             complement(1136..2440)
                     /locus_tag="VVN16_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5572494.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            2512..2889
                     /locus_tag="VVN16_001092"
     CDS             2512..2889
                     /locus_tag="VVN16_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU5572495.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKNSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2980..3312
                     /locus_tag="VVN16_001093"
     CDS             2980..3312
                     /locus_tag="VVN16_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU5572496.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3324..4895
                     /gene="ffh"
                     /locus_tag="VVN16_001094"
     CDS             3324..4895
                     /gene="ffh"
                     /locus_tag="VVN16_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5572497.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPNRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5090..6373)
                     /locus_tag="VVN16_001095"
     CDS             complement(5090..6373)
                     /locus_tag="VVN16_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU5572498.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKEKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6627..7340)
                     /gene="rsmG"
                     /locus_tag="VVN16_001096"
     CDS             complement(6627..7340)
                     /gene="rsmG"
                     /locus_tag="VVN16_001096"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU5572499.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7434..7994
                     /locus_tag="VVN16_001097"
     CDS             7434..7994
                     /locus_tag="VVN16_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5572500.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7996..8895
                     /gene="htpX"
                     /locus_tag="VVN16_001098"
     CDS             7996..8895
                     /gene="htpX"
                     /locus_tag="VVN16_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU5572501.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRIFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8939..10465)
                     /locus_tag="VVN16_001099"
     CDS             complement(8939..10465)
                     /locus_tag="VVN16_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5572502.1"
                     /translation="MIKINHLTITQNKDLRDLVSDLSMTIQDGEKVAIIGEEGNGKST
                     LLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFFLDSIDLDYSIL
                     YRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLDEPSNDLDLETV
                     DWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLVEHLDYESYSEQ
                     RKAKFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGRLLAKKMKTVLS
                     QEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLSIDDRVLVQKLQ
                     LTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHKKLQLDLSPIAY
                     LSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDLVLRKPNFLLLD
                     EPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSSIYRLTEHGLELVNLEDV
                     "
     gene            11224..11766
                     /locus_tag="VVN16_001100"
     CDS             11224..11766
                     /locus_tag="VVN16_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5572503.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11766..12395
                     /gene="nth"
                     /locus_tag="VVN16_001101"
     CDS             11766..12395
                     /gene="nth"
                     /locus_tag="VVN16_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5572504.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12606..13127
                     /gene="pyrR"
                     /locus_tag="VVN16_001102"
     CDS             12606..13127
                     /gene="pyrR"
                     /locus_tag="VVN16_001102"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5572505.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13146..14069
                     /locus_tag="VVN16_001103"
     CDS             13146..14069
                     /locus_tag="VVN16_001103"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU5572506.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14119..15198
                     /gene="carA"
                     /locus_tag="VVN16_001104"
     CDS             14119..15198
                     /gene="carA"
                     /locus_tag="VVN16_001104"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5572507.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15511..18687
                     /gene="carB"
                     /locus_tag="VVN16_001105"
     CDS             15511..18687
                     /gene="carB"
                     /locus_tag="VVN16_001105"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5572508.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTCAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAVRKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5451 a         3599 c         4187 g         5473 t
ORIGIN      
        1 ctactttaag atatgataaa gtcttatttt tagtgaagta tcctaatttg tataattgat
       61 tgtgtgtaga ttcagggtca atttgaagag agtatttaaa atatcttggg aaattataat
      121 ttctccctgt tttttgagtc caatctatga gtatgagttg cccaggagta aggccgcact
      181 ctaatttgtt taggtaaaaa ttgttacaat cagaatttgt gtctctatta tgattatttt
      241 gttttaaatt aaatagtttg ctaaaaaaat tcattggatt ttattgctaa tcaattctta
      301 ttgaaaaatg atacttttac agtacccatg tacggatggc atgggcaacg ccagattcat
      361 cattagattt agtgatgtat ttggcgattt ttttgatttc tggatttcca ttttccatga
      421 caacagggtt gccaacgact tccagcatgg cacggtcatt ttcttcgtct ccgatagcca
      481 tggtttcatc tttggttaat cctagttttt cagctaagtg agtaatagct gaacccttgt
      541 ctacattctt tttaaggagt tcgaggtaga aaggagcaga tttgttgata gagtagtgct
      601 cgtaaaattc ggctggtatt ttttcaatcg cagcatcgag aatttctggt tcatcgataa
      661 acatacattt aacaatttct ttgccagcca tttcttcagg ggtacggtag aagataggca
      721 tgctgacgag ggttgattcg tgtacagtgt atgttccgat attgcgattt gcagtataga
      781 taccgtcctt ggtaatggca tgcatgtgga caccgagctt gcgactgagg aattccatat
      841 ccagataatc ctcataggtc aaggattcgc tgataatctc atggccagta gcagtttctt
      901 ggacaagggc accattgaag gtcaccacat agtcaccttg gtctctcaac tgcaagtcgt
      961 ctagaagttt ggcaacacct gcgatggggc ggccagttgc aatcacgact ttgacaccag
     1021 cttctttggc atcttggatg gcagaaaaga cttcaggagt gatttccttt tggctgttga
     1081 caagggttcc gtcgatatca acggcaatta gtttaatact cataaatttc ctcttctagt
     1141 ttttatttgg ggtaaaatga tcgttctcaa tcaagtataa aaattgctgg gtaatgctag
     1201 caaagatact gttttgatct aacatttctt tcggaaaata gaaacgatta tctccgtgac
     1261 gactaccagc aagggattgg acgataggag acaggctaga gagttcggcc agttctccat
     1321 tcttttgtaa aatctcaatc tgtgtccgtg gattttcaga ttcgggacga tagatatcat
     1381 aagggaggtc aaagttctta tgaatggcag tatagtagtc gggatcaaag ccaatgtcct
     1441 caaccaattt tctcatgcta gcgagttggt cttgatcttc ttgtgaaaag gtaatggatt
     1501 taaagacctt gcggttgaca aagcgttgcg ataaatctgc gagaattttg tcaggactgg
     1561 tcatccagag ctggaagtag gtattcatca caccatcatc tagagccaga tagtcagaca
     1621 aggtcacatt tttttcaaag aaaggcagga ggtgtgggga agttcgtgca aagaagtctt
     1681 tgtccttagg gtagagttcc ttagcgcgct tgagaagatt ctgtaggaga acttccatag
     1741 cgcgtgttgc tgggtggaaa tagacctgca tatacatctg gtagcgactg aggacatagt
     1801 cttcgatggc gtgcatgcca ttgcgctgaa aggcgatacc attttcgata ggacgaatga
     1861 ctcggaggat tcgagtcagg tcaaattccc cataggatgc tcctgtaaaa taggagtcac
     1921 gcaagagata gtccatgcga tctgcgtcaa tctgactaga aatgagttgt acgacctgct
     1981 tgttaggata ggtatggtct atgacactgg caactttctc tggaaaatcg ggcgctactt
     2041 gtagcaagac ttggtgaatc tctgtctcag gactttgaat aatctcctga gtaatagctt
     2101 catggtctgt atcaaagaga tgttcaaaag tatgggagta ggcgccatgc ccaaggtcat
     2161 gtaggagagc agcgatcatg gtcaagagag actcggcagg attccattcc tcaggatatt
     2221 tttcttcgaa aatctctgtg atgcgtcgtg caatttcata gactcctaga cagtgagaga
     2281 agcgactgtg ttctccaccg tggaaggtat aactggaagt tcccagttgt ttgatccggc
     2341 gcaaacgctg aaattctttt gtattaatca agtcatagat gatttgatta ttgacatgga
     2401 tgtagttgtg aactgggtca cggaatactt tttcgttcat atgaccatta tagcaaaatc
     2461 ctgctctttt tggtaaaata gtaggacaag agacaagaaa gagggcctga tgtgaagatt
     2521 cggatgcgaa atacaattca gtttgatgag cagttggaag tgattgacca gctttatgat
     2581 gtggaagtgc atgaaaaagg agattatagc tacctgcttt tctataatga ggaaaaggaa
     2641 aaagtagtga ttaaatttca tggtcaagaa cttgtgatga gccgattttc taatcccaag
     2701 accattatgc gctttctaaa gaatagcgat agtttagcct atattcctac acctatgggc
     2761 atgcaggagt ttatcatcca aacgagccat tatcaagttg ataggcaaaa gattgaacta
     2821 gattatcaac tacaaaatca agagggacat ccttttgcca gctatcaatt ggaaattact
     2881 tggggctagg ctcatgtctt tttcaaattt agctatctat cttcttggat cggcttccta
     2941 gtgtggtaga ttaggctaat ttttggtata ataaaagtta tggaaatcga aaaaaccaat
     3001 cgtatgaatg cgctctttga attttatgcg gcgcttttga cagataagca aatgaattat
     3061 atcgagctct actacgctga tgattacagc cttgctgaaa ttgccgagga gttcggtgtc
     3121 agtcgtcagg ctgtctatga caatatcaag cgaacagaaa agattctgga agattatgag
     3181 atgaaattgc acatgtactc ggactatatt gtccgcagtc agatttttga tcagattttg
     3241 gagcgctatc ccaaggataa ctttctgcag gagcagatag aaattttaac aagcattgat
     3301 aatagagaat aagaggaaga aaaatggcat ttgaaagttt aacagaacgt ttgcagaacg
     3361 tctttaaaaa tctacgtaaa aaaggaaaaa tctctgaatc tgatgtccaa gaggcaacca
     3421 aagaaattcg cttggccttg ctcgaggccg acgttgcctt gcctgttgta aaggacttta
     3481 tcaagaaagt tcgtgagcgt gcagtcgggc atgaggtcat tgatacactt aatcctgcgc
     3541 aacagattat taaaatcgtt gatgaggaac tgacagccgt tttaggttct gatacggcag
     3601 aaattatcaa gtcacctaag attccaacca tcatcatgat ggttggttta caaggggctg
     3661 gtaaaacaac ctttgctggt aaattggcca acaaactcaa gaaagaagaa aatgctcgtc
     3721 ctttgatgat tgcggcggat atttatcgtc cagctgccat tgaccagctt aagaccttgg
     3781 gacaacagat tgatgtgcct gtctttgcac ttggaacaga agtaccagct gttgagattg
     3841 tacgtcaagg tttggagcaa gcccaaacta atcataacga ctatgtcttg attgatactg
     3901 cgggtcgttt gcagattgat gagctcctca tgaatgagct tcgtgatgtg aaagcattgg
     3961 ctcaaccaaa tgaaatcttg cttgtcgttg atgctatgat tggtcaggaa gcagccaatg
     4021 ttgcgcgtga gtttaatgct cagttggaag tgactggggt catccttacc aagattgatg
     4081 gcgatactcg tggtggtgcg gctctatcgg ttcgtcacat cactggaaaa ccaatcaagt
     4141 tcactggtac aggtgaaaaa attacagata tcgaaacctt ccacccagac cgtatgtcta
     4201 gccgtatcct tggcatgggg gatatgctca ctttgattga gaaagcttct caggaatacg
     4261 atgaacaaaa agcccttgaa atggctgaga agatgcgcga aaacaccttt gattttaatg
     4321 atttcatcga tcaattagat caggtgcaaa atatggggcc gatggaagac ttgctcaaga
     4381 tgattccagg tatggccaac aatccagcac ttcaaaacat gaaggtggat gaacgccaga
     4441 ttgctcgtaa acgtgccatt gtgtcttcga tgacacctga agaacgtgaa aacccagatt
     4501 tgttaaatcc aaaccgtcgc cgtcgtattg ctgctggttc tggaaataca ttcgtcgaag
     4561 tcaataaatt catcaaggac tttaaccagg ctaaacagct catgcagggt gttatgtctg
     4621 gggatatgaa taaaatgatg aagcaaatgg ggattaatcc aaataacctt cctaaaaata
     4681 tgccaaatat gggaggaatg gatatgtctg cccttgaagg aatgatggga caaggcggta
     4741 tgcctgactt atcagctctc ggaggagcag gaatgccaga tatgagccag atgtttggtg
     4801 gcggtttgaa aggtaaaatt ggtgaatttg ccatgaaaca gtccatgaaa cgtatggcta
     4861 acaaaatgaa gaaagcgaag aagaaacgca agtaagacaa gagattgatg caaatctcat
     4921 ttgatgagaa aataaagtaa aaatttctat gataaaacgt acagtatcaa ggttttagaa
     4981 tatctgatat tatacgtttt ttgattttaa taattttgtt aagacacttt tattatggta
     5041 tacgaaaaat ccactcagct gtctgagtgg attaggtgta tttagactct tagtctttat
     5101 tttcgtatgg caagatcaag ttcaagatga ttcctgtcat ggctgaaagg gcagtacctg
     5161 aaagtgtaac tggaccaagt ttaaggatag ctcctccaag tccaagaacc aacatagcac
     5221 ttgcgatgat gaggtttcgc atttgagcga aatcaacacg ttctttaatc aagactttca
     5281 aaccattgct ggcgataacc ccatagagaa ggattgacat accaccaagt acagcgtttg
     5341 gaatagttga aatcaaggca gtgaatttac caaggaagct gagggcaatc gcgatgaagg
     5401 cagcgttacg gataactgag acagaagcga tacgagtcat accgataacc cctgtatttt
     5461 ctccgtaagt tgtattggct ggtccaccaa ggaaggcaga aacagaagtt gcgataccgt
     5521 caccaataag agtacggtga agacctggtt cttttaagaa ttgacggcca cagatttgac
     5581 ccaaaacagt atggtctccg atatgttcag aaattgttac gatagcgatt ggcaagatag
     5641 cgatggcttc tggaccaaag taaagattgt actctttaaa ggcaccacct gtgctaaatg
     5701 gcaagtagaa accaggaatt tcaaaccagt tagcttctag gacaggcgta aagtcaacca
     5761 aaccaagagt tagtgcgaag aggtaaccac cgataatggc aaagaggaat ggaatgattc
     5821 gtaggaagcc tttttctttt gtattgataa aggcagcaat taggaaagta acaacggcta
     5881 ccagagcatt tttccaattt ccgtctgcta caagacctgc attggtaaca gctgaacctg
     5941 caagtccaag accgataacg atgatcatag gaccgataat gattggtggc aagagtttat
     6001 caatccattt tgttcctaca aatcggatgc tggtagcaac aaggacatag accaaaccag
     6061 tcaagataac ccctgtttgg gcagcagata catccccccc catttctttc atagccagtg
     6121 acatagctgt gataaaggcg aatgaagaac ctagataaac tggaacttta aaaccagtag
     6181 caatcatgta gatgagtgtt ccaacacctg aagcaaaaag ggcaacagat acaggcattc
     6241 ccaaaatcaa tggtaccaag atggtcgcac caaacatggc gaaaacgtgt tggaaactaa
     6301 ggagaattcc ttttccagcc gaaggacgtt ggtcaacgtc tagtaacaaa tcaacagttg
     6361 attcttgttt catataaacc tcacttttgg gcatcaaaaa agagcccatt attttacagc
     6421 aataggccct cagagtaaga cttctttaaa atttagattt taaagagttt taaatatttc
     6481 tgcgtcttcg tcacctcacg ggatgacatt aaaaaccttt gcttaggata gtatagcaga
     6541 aaaaaatgat tttgtaaatc attttttccc gagcctagaa ataaaagagc gaggctgatt
     6601 ttgtaaacct ttgtttacta aaaaatttaa agtgggcgtt tatttggcat accagcctta
     6661 cgtggatatt tatttggtgt ttcttttttc ttttctacca ctgtgatata gcgcggatct
     6721 ctattcggta gggcgtagct gagattgtct tcgaccttac taaaaaggag attgagggca
     6781 ttcttagctt ctaataattc ctcaggcgca ttgctagcct tgagtgctaa tagtttgcca
     6841 ccaaccttaa ggtagggaat agtcaattca gataggacct gcatacgggc aaccgcacga
     6901 gctgttacaa aatcatattg agcacggaag ttcttgtctt gggcaaaatc ttcggcacgt
     6961 ccgtggtaga aatgaactcc gttcaaatcc agttcttgag ccaagagttg taggaagttg
     7021 atgcgcttat tgagtgaatc aataatggtc acatctaact ccggatagag aattttcatt
     7081 ggtagactag gaaatcctgc cccagccccg atatcaagaa gtttgatagt ttcattggga
     7141 atcaaacctt gaagaatggg tgcaatcgaa tcgtaaaaat gtttgagata aacttcttcc
     7201 ttgtccgtaa tcgccgtcaa attaatcttc tcattccact cgaccaagag ctcaaaataa
     7261 cgttcaaatt gttctttttg ctggtccgaa agtggaagat tctgctcggc aagcaagttg
     7321 taaaatgttt ttggtttcat agttatttcc ttctttagta tcatcttatt ttaccacaat
     7381 catagaaaat ttgatatact ggtactaatc taaaagcaga aaggacttat attatgactt
     7441 ggattattct tggagtttta gctcttgttg ttatttttgt gattgttagc tataacggtt
     7501 tggttaaaaa tcgtatgcaa accaaggagg cttggagtca gattgatgtt cagttgaaac
     7561 gtcgcaatga tctcttgcca aacttgattg agactgtaaa aggttatgcc aaatatgaag
     7621 gttctaccct tgaaaaggtg gcagaactac gtaaccaagt ggcggcagcg acttcaccag
     7681 cagaagctat gaaagccagt gatgccctca ctcgtcaggt ttcaggtatt tttgcagttg
     7741 cagaaagcta tccagatttg aaagctagtg ctaactttgt taaattgcaa gaggagttga
     7801 caaacacaga aaataaaatt tcttactctc gtcaactcta taacagtgtt gtcagcaact
     7861 acaatgtaaa attagaaact ttcccgagca atattatcgc tggaatgttt ggatttaaag
     7921 cggcagattt ccttcaaaca cctgaagagg aaaagtcggt tcctaaagtt gattttagcg
     7981 gtttaggtga ctaagatgtt gtttgatcaa attgcaagca ataaacgaaa aacctggatt
     8041 ttgttgctgg tatttttcct actcttagct cttgttggtt atgcggttgg ttatctcttt
     8101 ataagatctg gacttggtgg tttggttatc gcactgatta tcggctttat ctacgctttg
     8161 tctatgattt ttcaatcgac agagattgtc atgtccatga atggagcgcg tgaggtggat
     8221 gagcaaacgg caccagacct ctaccatgta gtggaagata tggctctggt cgctcagatt
     8281 cctatgcccc gtattttcat tattgatgat ccagccttaa atgcctttgc gacaggttct
     8341 aatcctcaaa atgcggctgt tgctgcgact tcaggtctac tagctatcat gaatcgtgaa
     8401 gaactagaag ctgttatggg acatgaagtc agtcatattc gtaattatga tatccgtatt
     8461 tcgactattg cagttgccct tgctagtgct atcaccatgc tttctagtat ggcaggtcgt
     8521 atgatgtggt ggggtggagc aggtcgcaga cgaagtgatg atgaccgaga tggaaatggt
     8581 cttgaaatca ttatgctagt ggtttcccta ctagctattg tactggcacc tctcgctgca
     8641 accttggttc agctcgctat ttctcgtcag agggaatttc tggcagatgc atctagtgtc
     8701 gagctgactc gcaatcccca gggaatgatt aatgccctag ataagttgga caatagcaaa
     8761 cctatgagtc gccacgtcga tgatgctagc agtgctcttt atatcaatga tcccaagaaa
     8821 ggtggggggt tccaaaaact cttttatacc cacccaccta tctcagaacg gattgaacgt
     8881 ttaaaacaga tgtaaaagaa tccagaggtc gtctctggat ttttgctatg ttgcaaattt
     8941 atacatcttc taaattaact aactccaaac cgtgttctgt caagcggtag atgcttgaac
     9001 agacctcttt taagaaacga cggtcatgcg aaacagtgat gagaccgcct ggataggttg
     9061 caaagagttt tctgatttgg ggttgagaag tgggagaaaa gtttcgtgtg ggttcatcca
     9121 gcaggagaaa gtttggtttg cgcaggacta aatccaaaag caggagtttt ccctgttgtc
     9181 cgccagataa ggagcgaatt tgatgctgca tttctggata actgaaattg agactagcta
     9241 ggtgagattg gattttctgt agttcctctt tttccccagt tttgctgagg taggctattg
     9301 gtgataaatc caattgcagt tttttgtggt aatcttgtgg cataaaacca agggaaatct
     9361 ctcttttatc attcagaagt tgctttaatt tggctaacag agttgatttc ccaacaccat
     9421 ttggcccgat aataccgatt ttttcttgcc cacggacagt tagttgtagt ttttgaacca
     9481 aaactcggtc gtcaatggac aaattttctt tttccagttg gactaagact ttagaagctg
     9541 gtaatggttg gatgtctgaa aagaaaagtt ggatttgttc ctcttcaagt ggcttttgag
     9601 tcatggactg agctgccttt tcgtagcgtt tttcttgtga gaggacagtt ttcatctttt
     9661 tagccaatag gcgaccggca gtactgtctt tggtagctcg aagcgcagtt tctacatttt
     9721 gtttgactct ccgatgtttt tccatggttt tatcgtaggc tctttggttg ttagcagctt
     9781 gctgactttg tttggcaaat ttagcctttc tctgctcact atagctttca tagtctaaat
     9841 gctctactag cgtttccgct tctttacggt gtttgaccag tcgcaagtga acaatagtgt
     9901 ctgccgtttc agaaagaaag tcttcatcat gggaaatgaa aataacggtt tgcctggtct
     9961 tttgaatctg gccttttaac caatcaactg tctcaaggtc taggtcattt gaaggttcat
    10021 ctaaaaatag aatctcaaag ggtttggcta actcatggat aagctgaatt ttcaaagctt
    10081 ctccccctga tagattgcca atctcttggt cacttgcgaa acgattgcta tcaaaatgca
    10141 attcctccgc caaacgatag aggatactgt agtctaaatc aatagaatct aaaaagaagt
    10201 agtcgtgtaa agttttcttt ttcagctcct cgggaagttt ttgaggaatg taggccagtg
    10261 actgataatc agactggatg tctcccttga tagtgaaatc agacaaatct tcccccatta
    10321 aagttttaag taaggttgat ttaccatttc cttcttcgcc aataatagct accttttccc
    10381 cgtcttgtat ggtcatgctt aggtcagaga caaggtctcg taaatctttg ttttgtgtga
    10441 tggtcaggtg attgattttt atcataacta ttgccctttc tagaatgttc atagtgcata
    10501 acaaaaagct ctactttacc tagtagagcc caaagtctat gtcaaaaaaa ctctattaac
    10561 atgccagaaa aagggcataa aagagcacac aaaaagagac aaaaacaaga tctactaaat
    10621 aaaggttctt tatttgatag acttagttgt tcatgtctcc cttacctcct agtattagta
    10681 tctttatact atacttttaa ggaaattttg tcaacagaaa actggaaatt ctatgctcta
    10741 aaatccaaaa acaggtctat agagacttag tacgtgtttg acatgctcgt aatggatttt
    10801 atcatagttt cgccaagcag tgcgttcttg attgtttagt tttaggctac ccagtccaat
    10861 cataggactg gtgggttggt aaaatttctc aagatcaatt aagatactgt tgttcaactt
    10921 ttccgttttt tgagttcttg catagtagtg tttagcagct cttgtagtcg aatatcatct
    10981 gccgtaccct gtttacagtt ttggtagctt ttatttaact tggataggag ttgaatgagt
    11041 ttcttgtctt ccgtgtgagc atcctccttg atctgatgta ctctttccct atagtatagc
    11101 actaaaaaat aaaactgtca tagtgaatat gttaaaattt cgaattttaa ttacaaagtt
    11161 tggtgtgctg atttgtagcc ttttcatggt ataatcaaga gagtaaatct ttatggagga
    11221 agtatgaaat taaatattca agaaattcgt aagcagtctg aaggtttgaa ctttgaacaa
    11281 acgttagatt tagttgatga cctgcgtgca cgtaatcaag aaattttaga tgtaaaagat
    11341 atccttgcag ttgggaaagt acaatatgaa gaccgtatgt atttcttaga ttatcaacta
    11401 tcttatacca ttgttcttgc ttcgagtcgc agtatggagc cagttgagtt agttgaatct
    11461 tatccagtca cggaagtttt catggaaggc gcaactaacc agctagatca agaagtttta
    11521 gatgatgact tggtcttgcc catcgaaaat ggggagcttg accttgctga gagtgtatca
    11581 gacaatatcc tgctaaacat tcctatcaag gtcttgacgg ctgaagaaga agctggtcaa
    11641 ggatttgtct caggaaatga ctggcaaatc atgacagagg aagaatacca agctcaacaa
    11701 gcagtaaaga aagaagaaaa cagtcctttt gctggcttac aaggactatt tgacggagat
    11761 gaataatggt cttgtcaaaa aaacgagcac gaaaggtgct agaagaaatc attgccctct
    11821 tcccagatgc caagcctagt cttgatttta ccaatcattt tgaactcctg gttgcggtca
    11881 tgttatcagc ccagacgaca gatgcagcgg taaataaggc cacaccaggt ctctttgttg
    11941 cctttccaac accccaagct atgtctgtag cgacagagag tgagattgct tcacatattt
    12001 ctcgcttggg attgtatcgg aataaagcta aattccttaa aaaatgtgcc caacagttac
    12061 tagacgattt tgatggtcaa gtccctcaga cacgtgaaga attggagagt ttggcaggtg
    12121 ttggtcgcaa gacagccaat gttgtcatga gtgtaggatt tgggattcca gcctttgcag
    12181 tggatactca tgtggagcgt atttgcaaac accacgatat tgtcaaaaaa tcagcgacgc
    12241 cacttgaggt ggaaaagcgg gtcatggata tcttgccgcc tgagcagtgg ttagctgccc
    12301 atcaggccat gatttatttt ggaagagcca tttgccatcc aaaaaatcca gagtgtgacc
    12361 agtacccaca attatatgat tttagcaatt tgtaagatgc tacattgaaa ctttttgctt
    12421 gattttaagc atgctttgtg ctatagtaaa tgataactaa atattccttt aaacctgtcc
    12481 cgtgaggcag gcaaggagcg cgataaagta gaagggtgaa gtctatactg tgtgcagtag
    12541 ggctcgcttg gctataaatt tcaagtctcc ttgtttaagg agacttttct ttttggaggt
    12601 ttgtcatgaa aacaaaagaa gttgtagacg aattgaccgt caaacgagcg attacgcgta
    12661 ttacttatga gattatcgaa cgcaacaaag atttgaataa gattgtcttg gctggtatta
    12721 aaactcgtgg tgtctttatt gcccaccgaa tccaagaacg tttgaagcag ttagaaaatc
    12781 tttcagttcc tgttgtggaa ttggatacta aacctttccg tgatgatgtt aaaagtggag
    12841 aagatacttc tttggtttct gtcgatgtga cagaccgcga agttatcttg gtggatgatg
    12901 tgctctatac aggtcgtacc atccgtgctg ctattgataa tattgtcggt catggtcgtc
    12961 ctgcgcgtgt gagtttagca gttctagtcg atcgtggaca tagagaattg ccaatccgtc
    13021 cagattacgt tggaaaaaat atcccaacca gtcgttctga agaaatcatc gtagagatga
    13081 cagaacttga tgaccaagac agagttctga ttactgaaga agcttagaaa gttaaaggag
    13141 tagccatgtc agaaaatcaa caagcattga accatgtggt gtccatggaa gacctcactg
    13201 tcgatcaagt aatgaaattg atcaagcgag gaattgagtt taaaaatgga gcccagcttc
    13261 cctatgaaga ccatccgatt gtttccaatc tcttctttga ggattctaca cggacacata
    13321 agtcctttga agtcgcagag attaaacttg gattggaacg acttgacttt gatgtgaaga
    13381 ctagttcggt taataagggt gagacacttt atgacaccat tttgactctg tctgctttag
    13441 gagtggatgt ctgtgtgatt cgccatccag aggtcgacta ctatagagaa ttgattgcta
    13501 gtccaacgat tacgacttcc atcatcaatg gtggagatgg ttcgggccaa caccctagcc
    13561 agagcttgct tgatttgatg accatttatg aggaatttgg ccactttgag ggtcttaaag
    13621 ttgctattgc aggtgacttg gaccactcac gcgttgccaa atccaatatg cagattttga
    13681 aacgcttggg atctgaactc tttttcgctg gacctgagga atggagaagt caagagtttg
    13741 cagactatgg acagtttgta accattgatg aaatcattga tcaggttgat gtcatgatgt
    13801 ttctccgtgt gcaacacgaa cgccatgata gtggagctgt attttcaaaa gaagactacc
    13861 atgcccaaca tggcttgact caagaacgtt acgatcgttt gaaagaaaca gcaatcctca
    13921 tgcacccagc tccaatcaat cgtgatgtag aaatagcaga ccacttggtt gaagcaccaa
    13981 aatcacggat tgtccaacaa atgaccaatg gtgtctttgt tcgaatggca atcttagaat
    14041 ccgtactagc gagtagaaac gccaactaaa acacaagacg ttaaaagacg ccagtgagcg
    14101 tccacgagag gtgaaaatat gacaaaaaga attctagtat tagaagatgg cacagttttt
    14161 gaaggcaagg ccttcggagc agatattgat gtaacaggcg aaatcgtctt taatacaggg
    14221 atgaccggct accaagaatc cattacagac cagtcttata atggacaaat cttgaccttt
    14281 acttatcctt tggtaggaaa ttatggtatt aatcgtgatg attatgaatc cattattcca
    14341 acttgtaagg gagtcgttgt ttttgaagaa gcgcgtagag ctagcaactg gcgcaaccaa
    14401 atgaccttgg atgaattttt gaaagccaag aaaattccag ggatttcagg aattgatacg
    14461 cgtgctctta ccaagattat ccgtaagcat ggtactatgc gtgcaacctt gacccatgtt
    14521 ggggacagta tggaccatgt gacggaccag ctccaagcaa cagttttacc gacagacaat
    14581 atcaaacagg tttctactaa aacgtcttat ccagctccag gagttggttt gagcgtggtg
    14641 ctagtggact ttggtctcaa gcactcaatc ctacgtgaac tttctaaacg caactgtaac
    14701 gtgacagttg ttccttattc gacaacggct gaagaaattc tccatctcaa tcctgacgga
    14761 gttatgttgt caaatggtcc aggtaaccca gaagacgttc cacaagcact cgacatgatt
    14821 cgcggtgtac aaggaaaaat tccaatcttt ggtatttgta tgggacacca actctttgca
    14881 atggcaaacg gggctaagac ctataagatg aagtttggcc accgtggatt caaccatgcg
    14941 gtacgtgaaa tcgcaacagg acgagtagac ttcaccagcc agaaccatgg ttatgcagtc
    15001 agccgcgagg atttgccaga gcacttgatt attacccacg aagaaatcaa tgacaagtca
    15061 gtggaaggtg tgcgccacag ataccaacct gctttctctg ttcaatacca cccagatgca
    15121 gctcctgggc cacacgacgc tagctaccta tttgacgagt ttatcgagat gatggaagtt
    15181 tttaaacaat caaactaaga acgagtaaaa gctcgtcgga gaatttatgt aactgaacac
    15241 ggacggaaag ctcagaatgt agagaaacca acttggattg tcaatccttc cttgatttcc
    15301 taaaattcaa tcgctttcta ttcgtccttt gtatcttatt taatgtcgcc ctgagaggcg
    15361 acgaatagaa aggagaaggg tgctccgtat ttgctgaact gaatacgggc tacggacggt
    15421 gctaaaaaga tagttattcc tagaactgac agttcttcgt cataactcct attttagctt
    15481 tgtccgcttg acacccttaa tatcttatat atgcctaaac gtactgatat tcaaaaaatt
    15541 atggtgattg gttctggtcc gattattatt ggtcaggctg ctgagtttga ctacgctggg
    15601 actcaggctt gcttgtcgtt gaaagaggaa ggttatgagg ttgttttggt taactcaaat
    15661 cctgcaacca tcatgacgga caaggaaatt gctgacaagg tttacattga accgattaca
    15721 cttgagtttg tgacacgtat tcttcgtaag gaaggtccag atgccttgct accaacactc
    15781 ggtggtcaga caggtctcaa catggccatg gaattatcta aaaatggtat cctagacgag
    15841 cttggcgttg aacttctggg taccaaatta tctgccattg accaagcgga ggaccgtgac
    15901 ctctttaaac aattgatgga agagcttgaa caacccattc cagaatctga aattgttaac
    15961 acagtagaag aagcagttgc ctttgcagcg acaattggct acccagtcat cgttcgtcca
    16021 gcctttaccc ttggtggtac tggtggtggt atgtgtgcca acgagaaaga attgcgtgaa
    16081 atcactgaaa atggtttgaa attgtcacct gttacccaat gtttgattga gcgttcgatt
    16141 gccggtttca aggaaattga atacgaagtg atgcgtgact cagctgacaa tgccttggtt
    16201 gtttgtaaca tggaaaactt tgacccagtt gggattcaca caggggattc tattgtattt
    16261 gcccctgcgc aaaccatgtc agactatgaa aaccaaatgc ttcgtgatgc gagcttgagc
    16321 attattcgtg ccctcaagat tgaaggtgga tgtaatgttc agctagctct tgatcctaac
    16381 agcttcaagt attatgttat cgaagtaaac cctcgtgtat cacgttcgtc agcccttgct
    16441 tctaaagcga caggatatcc gattgctaag ttggctgcca agattgcagt aggtttgacc
    16501 ttggatgagg ttataaaccc agttacaggt tcaacctgtg ccatgtttga accagccctt
    16561 gactacgtag ttgctaagat tccacgtttc ccatttgaca agtttgaaaa gggtgaacgc
    16621 cgtctcggta cacaaatgaa ggcgactgga gaagtcatgg caatcggtcg taacatcgag
    16681 gaatctcttc tcaaggcctg ccgctccctt gaaattgggg tgcaccacaa tgaaatacct
    16741 gaacttgcag cagtttctga tgatgccttg attgaaaagg ttgtcaaagc ccaagatgac
    16801 cgtctcttct acgtatcaga agccattcgt cgtggctaca cacctgaaga aattgcagag
    16861 cttacaaaaa ttgatatctt ctatcttgat aaactcttgc atatctttga aattgagcaa
    16921 gaattgggtg ctcatccaca agatctagaa gttttgaaaa cagccaaact taatggtttt
    16981 tcagaccgta agattgctga actctgggga acgactgatg ataaagttcg ccaacttcgc
    17041 ttggaaaaca agattgttcc agtttataag atggtagaca cttgtgcggc agagttcgac
    17101 tccgaaacgc catatttcta ttcaacctat ggttgggaaa atgaatctat cagatctgat
    17161 aaagaatctg tacttgtcct aggttcaggt ccaatccgta tcggtcaagg ggttgagttt
    17221 gactacgcaa ccgttcactc tgttaaggct atccaggcag ctggttatga agctatcatc
    17281 atgaactcaa acccagagac cgtttcaaca gacttctctg tatcagataa gctttacttt
    17341 gagccattga cattcgaaga tgttatgaac gtcattgact tggaacaacc aaagggagtt
    17401 atcgttcagt tcggtggtca aacagccatc aaccttgcgg agccattggc aaaagcaggt
    17461 gtgaccatcc ttggtacaca ggttgctgac ctagaccgtg ccgaagaccg tgacctcttc
    17521 gagcaagctc tcaaagaatt ggatattcca cagccaccag gacaaacggc taccaatgaa
    17581 gaagaagcag ctcttgcagt tcgtaagatt ggcttcccag ttcttgtccg cccatcttat
    17641 gtacttggtg gacgtgccat ggaaatcgtt gaaaacgaag aagaccttcg ttcttacatg
    17701 cgaactgctg ttaaggctag tccagaccat ccagttcttg tcgactctta tatcgttggg
    17761 caagagtgcg aagttgatgc catttcagat ggtaaaaatg tactcattcc tggtatcatg
    17821 gagcatatcg aacgtgctgg tgtccactca ggtgactcaa tggctgttta cccaccacaa
    17881 accttgtcgc aaaaggttca ggagaccatc gcagactaca ccaaacgctt agcaatcggt
    17941 cttcactgcc ttggaatgat gaacatccag tttgttatca aggatgaaaa agtctacgtt
    18001 attgaggtca atccacgtgc cagccgtacc gttccattcc tatctaaggt aaccaatatt
    18061 cctatggctc aagtagcgac caagctcatt cttggtcaaa gtttatcaga acttggctac
    18121 caaaatggac tttaccctga aagcacccgt gttcatatca aggcacctgt cttctccttt
    18181 accaaactag ctaaggtaga cagcttgctc ggtcctgaaa tgaagtcaac aggtgaagtt
    18241 atgggttctg atgctacttt ggaaaaagct ctctataaag cctttgaagc ttcttatcta
    18301 cacttgccaa cttttggtaa tgtcgtcttt accatcgctg atgatgccaa agaagaagcc
    18361 ttgaacttgg ctcgtcgttt ccaaaacatt ggttatggaa tccttgcgac agaagggaca
    18421 gcagccttct ttgctagtca tggcttgcaa gcccaacctg ttggtaagat tggtgatgac
    18481 gataaggata tcccaagttt tgtacgcaaa ggaagaattc aagctatcat taacacagta
    18541 ggaacaaaac gaactgctga cgaagatggt gagcaaattc gtcgttcagc cattgaacac
    18601 ggggtgcccc tcttcacagc gctagataca gctaatgcca tgcttaaggt acttgaaagt
    18661 cgtagctttg ttacagaagc gatttaaata gaaaaataag atatagcaga
//
LOCUS       DAVHIT010000036        18120 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVHIT010000036 DAVHIT010000000
VERSION     DAVHIT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.037"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VVN16_001106"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VVN16_001106"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU5572509.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VVN16_001107"
     CDS             1377..1886
                     /locus_tag="VVN16_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572510.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4368
                     /locus_tag="VVN16_001108"
     CDS             1963..4368
                     /locus_tag="VVN16_001108"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5572511.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKTIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYIATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4436..5437)
                     /locus_tag="VVN16_001109"
     CDS             complement(4436..5437)
                     /locus_tag="VVN16_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5572512.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPINSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5593..5996
                     /locus_tag="VVN16_001110"
                     /pseudo
     CDS             5593..5996
                     /locus_tag="VVN16_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6122..6388
                     /locus_tag="VVN16_001111"
     CDS             6122..6388
                     /locus_tag="VVN16_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5572513.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6649..8898
                     /gene="metE"
                     /locus_tag="VVN16_001112"
     CDS             6649..8898
                     /gene="metE"
                     /locus_tag="VVN16_001112"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU5572514.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8962..9828
                     /gene="metF"
                     /locus_tag="VVN16_001113"
     CDS             8962..9828
                     /gene="metF"
                     /locus_tag="VVN16_001113"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU5572515.1"
                     /translation="MSCQTPSLSFEVFPPNPAVGNDNIILALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNEHFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10513..12726
                     /gene="pnp"
                     /locus_tag="VVN16_001114"
     CDS             10513..12726
                     /gene="pnp"
                     /locus_tag="VVN16_001114"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU5572516.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12742..13359
                     /gene="cysE"
                     /locus_tag="VVN16_001115"
     CDS             12742..13359
                     /gene="cysE"
                     /locus_tag="VVN16_001115"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU5572517.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHSFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHAVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13371..14255
                     /locus_tag="VVN16_001116"
     CDS             13371..14255
                     /locus_tag="VVN16_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572518.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14342..15685
                     /locus_tag="VVN16_001117"
     CDS             14342..15685
                     /locus_tag="VVN16_001117"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5572519.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15678..16064
                     /locus_tag="VVN16_001118"
     CDS             15678..16064
                     /locus_tag="VVN16_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU5572520.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16068..16952
                     /locus_tag="VVN16_001119"
     CDS             16068..16952
                     /locus_tag="VVN16_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU5572521.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17089..17688
                     /locus_tag="VVN16_001120"
     CDS             17089..17688
                     /locus_tag="VVN16_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572522.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            17831..18001
                     /locus_tag="VVN16_001121"
                     /pseudo
     CDS             17831..18001
                     /locus_tag="VVN16_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         5465 a         3490 c         4092 g         5073 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagtg gtaattgaat agaaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctgac tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaat gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caaacctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaagctt
     1501 atagtctaaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gttaggtgtt tgggagcata atacaaaagc tcaagcgttt tataatcgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag aggattcttc tattgaaatg ataggaaagg
     1921 aaaagaacta gagtgacaac tgtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggga tcgaggtaga gagtgtcgaa tcaccagctg ctgatctctc aaaaattgtt
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc acctccatgt ttgtcaggtt
     2161 aacgttggcg aagaagaaga gcgtcagatc gtttgtggtg ccccaaatgt gcgtgctggg
     2221 atcaaggtca tggtggctct tccaggagct cgtatcgctg acaattacaa aatcaaaaaa
     2281 ggaaaaatcc gtggcttaga gtcacttgga atgatctgtt cacttggtga attgggtatt
     2341 tctgactcag ttgtacctaa ggaattcgca gatggtattc aaattttgcc agaaaatgcc
     2401 gttccaggtg aggaagtctt ctcatatcta gacttggatg atgaaatcat cgaactttcc
     2461 atcacaccaa accgtgcaga tgccctttct atgtgtggag tggctcacga agtggcagcc
     2521 atctatgaca aggcagtcaa ctttaaagaa tttactctaa cagaaactaa tgaagctgcg
     2581 gcagatgccc tttctgtcag cattgagaca gacaaggcgc cttactatgc agctcgtatc
     2641 ttggacaatg tgaccatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggcatccgtc ccatcaataa cgtagtggac gtgacaaact acatcctgct ctattttggt
     2761 caaccaatgc atgcctttga cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcgt gttgtccttg aagctgctgt tttcaatggc
     3001 aaaactatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcctgatt
     3121 gcggaacttg caggtgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatact
     3181 tcagatgtag aagtttcttc aacccttgct gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtcgaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagagt cccacgtcgt cgttgggata tcacaatcga agctgacctc
     3361 tttgaagaaa ttgctcgtat ctatggttat gaccgcttgc caactagtct accaaaagac
     3421 gatggtacag caggtgaatt gacagccaca caaaaactcc gccgtcaagt tcgtactatt
     3481 gctgaaggag caggtttgac agaaatcatc acctatgctc taacaactcc tgaaaaagca
     3541 gttgagttca cagctcaacc gagtaacctt actgaactca tgtggccaat gactgtggat
     3601 cgttcagtcc tccgtcaaaa tatgatttca gggatccttg ataccgttgc ctacaacgtg
     3661 gctcgtaaga ataaaaactt ggccctttat gagattggaa aagtctttga acaaacaggt
     3721 aatccaaaag aagaacttcc aaatgagatc aacagctttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaggga
     3841 atccttgaag ccctatttac tcgtttgggc ctccaagtaa cctatatagc aacatctgaa
     3901 atcgctagcc ttcatccagg tcgtacagcc gtgatttcac tcggtgacca agttcttggt
     3961 ttccttggcc aagtgcatcc agtcactgcc aaggcttacg atattccaga aacgtatgta
     4021 gctgagctta acctttcagc catcgaaggg gcgctccaac ctgctgttcc atttgtggaa
     4081 atcaccaaat tcccagcagt cagccgtgac gttgcccttc tcctcaaggc agaagtgact
     4141 caccaagaag ttgtagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg tcttctcagg tgaaaaactg ggacttggta tgaagtcaat ggcttatagc
     4261 ttgaccttcc aaaatccaga agacagctta acggacgaag aagtcgcacg ctacatggaa
     4321 aaaatccaag cgtctctcga agaaaaagtc aatgcagaag tgcgttaaac gatagtagga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa aataattaaa
     4441 atgtgatttc tgtaaaaata catctatgat cagagttttg aaaacttaca atgtctaaat
     4501 ctttaacata gtagtggttt ttaggcaata aaatatgatc tattgttgca ttaaaaagtt
     4561 ttggactttg gctattccaa gttcctcttt caaaaggtgg cagtacattt aatgcgtccc
     4621 tatgagagct tatttttgca agtgctccat gacgcatagt tgcattaaaa tcacctgcaa
     4681 taatagcctt tggatatttt gaagccaatt gattatgaat gatgtttaag tcttgcttcc
     4741 agatttccat taaacctggc agaggaggag cagtatgcaa ggcaatgata tctggtatat
     4801 tttgttttct cgaatgtaat acaattgtcc cgaaccgtgt tgtatgaaaa gttttagctt
     4861 ctgtatagaa accataactt ttcttgacaa tcacagtcac aggagctatt ccactattga
     4921 taggtggaga agtgaaaata tcatagttgg ccatagaaag tccaacttga tgaaacaata
     4981 gtttgattct ctggttttct tgctcacctc tgatattggt agctagttca gggaatatag
     5041 ccatatcggc gtcaaaatgg ctaaaaattc gctcaatatg ttgtgcttct atttgattag
     5101 cgacgttcca agttactaat tttaaggttt tagtcttatt tgatgcagaa gaaagggttt
     5161 tatctgttcc gaataatagt aatatgagcg atagtaagag caaaatgcca gagtaaaacc
     5221 gtatttgttt atttttcttg taaaaagata ggaaaaaaaa tacgaaactt aaaatagcag
     5281 ccatagttgc agtagctaag gggaaggcat aaatctgtgc caagataggt tgggtagata
     5341 gccaaatgtt aagattagaa gatgggaaaa aagaataagt tatgataggg atagaaaaga
     5401 gaatgaccga aagggcagag agaaatgttt taatcattgt gtgagtacct caatttgcaa
     5461 tggttgctcg gagctaatgt gactttgtga ctttacttga ttgatattct ctttcttgtc
     5521 ttcctcatag attaagtaca acccaagtaa aaaaagtcca atttaagatt gaaatgatta
     5581 aaaggcacag aaatgcaaga caattatact acaaaaggca aacatttgac aatcgatagc
     5641 cgtcgcttaa tcgaacgatg gaaaaaagaa ggaaaatcaa atagagaaat tgcctctcta
     5701 cttggaaaag ctcctcaaac tatccacact gaaatcaagc gtgggacagt ccgacaatgt
     5761 cttggaaaag ggcgcttcaa agagatttat tctgccgact acgctcaaca gtcttaagaa
     5821 actaaaggaa aagattctcc actatacata accaaaaatt ttcgcctgaa atgatggtga
     5881 tagctaaagg ggttaacgtg ggaatttcaa ccatttacta ttggattcat cgtggaaaat
     5941 tggggttaag caagcaggat ctgctttatc ctagaaaagg aaaatcgctt aagaaatagg
     6001 ctagcaccaa ctttaaaccc actggtcaat ctatcgaatc ataattcgct cattttttta
     6061 aaaatacata gacgtaaaaa aaagagggtt aagtgatgaa gaatagtgct attgggagta
     6121 attggaagga tgtccatctg agctctttag caaggaggaa atccttgaaa gtgatatgcg
     6181 agtggctatc atgagcgagt tgattgaggc taggtatgaa caaggaatca gtcagaaaaa
     6241 gctggaagaa gtcagtggaa taagccagcc tgtcatagct aggatggaga caggaaagac
     6301 tagtcctcag ttggatacag tcttaaaagt cctagccagt ttaggaaaga cactagcaat
     6361 cgtcccactt gaacagggaa aaagttgact agaatcagtt atagtctttt ctaataacag
     6421 gtcatagtca cttataagaa ttactaataa catgttttag tattctaatt ggaatatagc
     6481 tgtaaagtaa gaattacaaa ggatttgaga cgtttgcttc aagtcttttt cttttgtccg
     6541 aaacagatat atagtttcaa actatatcaa accctaattt tttacaatta tacagacgct
     6601 ttcttttctg ttaagatagt ttcaacaaca aattttggag gacacatcat gtcaactaca
     6661 atcatcggtt tccctcgttt gggcgaattc cgtgaattaa aatttacaac tgaaaaatac
     6721 tttagaaaag aaatctcaga agaagaactt ttagcagcag caaaagactt gcgtgctaaa
     6781 cactggaaca ttgtcaaaga aaaaggcatc actgaaattc catcaaatga cttttctcac
     6841 tatgataact tcctagatgc ggctttcctt ttcaacgtgg tgcctgcatc cgttcaaaac
     6901 ttggacttgt ctgaccttga gcgctacttc gctttgggac gtggttacca gggagaaaaa
     6961 ggggacgttc gcgcccttcc aatgaagaaa tggttcaaca ctaactacca ctacatcgtt
     7021 cctaaatttg aaaaagacac tcaagtaaaa ttggctggtc acaagatttt cgatgagttc
     7081 caagaagcaa aagaacttgg attgaacact cgtcctgttc ttgtaggtcc cttcactttc
     7141 cttcaattgt cagactttga agaaggcgtg aaagcagacg acttcgtaga tagcttagta
     7201 gttgcttacc aagaagtttt tgctaaattg gctgaacttg gtgcgactcg catccaattg
     7261 gatgaagcgg ctcttgtaaa agacttgaca gctgaagaaa aagctctctt cttgaacctt
     7321 tacaacaaac tcttggctga caaaaaaggt cttgaagtct tgcttcaaac ttatttcggt
     7381 gatgttcgtg acgtatacgc tgaccttgta aacttaccag tagatgctat cggtcttgac
     7441 tttgttgaag gtaagaaaac tcttgaactc gttaaaggtg gcttcccagc tgacaagact
     7501 ctctatgcag gtattgtcaa tggtaaaaac atctggcgta acaactacga aaagagcttg
     7561 gctgttcttg agcaaatccc agctgaaaac atcgttttga caagctcttg ctcacttctt
     7621 catgtgccat ttacaactgc taatgaagaa tttgaaccag cactcttgaa ccactttgcc
     7681 tttgcagttg aaaaattgga tgaaatccgt gacttggatg ctatccgcaa tggtcaaggt
     7741 tcagaagctc ttgcagccaa caaagaactc tttgcgactg agcgtgttgg tgaaaatgct
     7801 gaacttcgtg cgcgtatcgc tggcttgaca gacgcagact acactcgttt gccagccttt
     7861 gcagaacgtg aagctatcca agaagaagct tttaaacttc cagctcttcc aacaacaacg
     7921 attggttcat tccctcaaac aaaagaagtt cgtgccaaac gtttggctta ccgtaaaggt
     7981 gaattgtctc aaaaagagta cgacgctttc cttgctgaaa cgattgacga atggatcaag
     8041 tggcaagaag atattgactt tgatgtcctt gttcacggtg aatttgagcg taatgacatg
     8101 gttgagtact tcggtcaaaa cttgtcagga tacctcttct ctaaaaatgg atgggtacaa
     8161 tcatacggta tgcgtggggt aaaaccacca atcatctggg gtgatgtcac tcgtcttaac
     8221 cctatcactg ttaaatggtc tagctatgca caaagtcgta caaacaaacc tgttaaaggt
     8281 atgttgactg gacctgttac catcctcaac tggtcattcc cacgtgaaga catctctatc
     8341 aaggattcta ctcttcaaat cgcccttgct atcaaggatg aagtgcttga ccttgaagct
     8401 gctggtgtga aaatcatcca aattgatgag gctgctcttc gtgaaaaatt gccactccgt
     8461 cgtagtgact ggtacgaaga ttaccttgac tgggcaattc ctgccttccg cttggtacac
     8521 tcaacagtag cgtcagacac acaaatccac actcacatgt gttactcaga atttacagat
     8581 atcatcccag ctatcgataa catggatgca gacgttattt cctttgaagc cagccgttca
     8641 aaccttgaaa tcttggacga actcaaagcg aaaaacttcc aaacagaagt gggacctggg
     8701 gtttacgata tccactcacc tcgtgtacca aatgaaggcg aaatcgacaa cacaatcgaa
     8761 gccatccttg ctaaagtgcc aagcaagaaa gtttggatca accctgactg tggtttgaaa
     8821 acacgtggta tcccagaaac aaaagaaagc ttgatccgcc ttgttgaagc agctaaagct
     8881 gcgcgtgaga aattgtaaga ttggatttct ctccatacag tggttgttca aaaagaaatc
     8941 aactagaaaa ggttattaca tatgtcatgc caaacaccgt cactctcatt tgaagtgttc
     9001 cctccaaacc cagccgtggg taatgataac attattttag ccttgcagga tatgcaggag
     9061 ttggctcccc attttatcag tgtaactgcc agcaataata aatttaatat caaggaaacg
     9121 acggttcgtt tggctgactt tatccaaaat gacttggcga ttccgaccat tgcccacttg
     9181 ccagctattt atctaactaa ggacaaggtt gctgaaacta ttgctgactt ggacaaagtt
     9241 ggggtgcaga aaatcttggc tcttcgtggg gatattattc cagacgtgga accacaaaag
     9301 gatttccgct atgcaaccga cttgattgag cttatcaagg aacaagcccc tcactttgat
     9361 attattggag cttgctatcc agaagggcat ccagattcgc caaatcagat ttcagatatt
     9421 caaaatctta agaagaaagt ggatgcaggc tgttcgagcc tcgtaactca gctcttcttt
     9481 gacaatgagc acttctatga ttttcaggac aagtgcatct tggctgggat tgatgttccc
     9541 attcatgcag gaattatgcc aattctgaat cgaaatcagg ctctccgact cttgaagact
     9601 tgtgagaata tccatcttcc acgcaaattt aaagccatct tagacaagta tgagcatgac
     9661 cctgagtcgc tcagagcagc aggacttgcc tatgcagtgg accaaatcgt agacttggta
     9721 actcaggatg ttgccggtgt gcatctctat acgatgaata atgctgatac agcaaaatac
     9781 atccatcaag caacccatgc cttgtttaat caccagtctc taggataata aaagcaaacc
     9841 attcttctca ggtgagggga atggttcctt tttaatggta aagacctcac tttttaagaa
     9901 aaatatgata aaataaattc tgtacgtact tgatacaaag atgaaggtat taaataatac
     9961 aatgcattta aactgtaaga tttaacgaca cgagtgagtc aaatcgatgt tatttgacga
    10021 gagagttagt aaagcattta gctaatcgcc cgatagcgat tagcgtgaaa gctttagata
    10081 ctttagtatt aaacctttct aatgatgtat cttgaaaact gaacttgcct acaactctgt
    10141 ggaaaaagat aaatctccct aggagcagtc gctccgtcgt tcgatttcct atcttccttt
    10201 gagttgcttg acggcttggt atcttgatga cttgaatttt ggtctcattt cttaggaaaa
    10261 aagataagct tcctagcact cgtcgagtgc gaagtcacct tcctattttt ctacagaaat
    10321 gttcggcaag ccgaaccgtc caaaatatct tgtgtaattg aacacggccg aaaagctgtg
    10381 taaaaaagat aaactgtctt gtcttcatcg aagacttcgt cagtttccta tttttacttt
    10441 gcttttgacg tccttggtat cttgatcttt gtaggcaagg cgtataattt catcaatcca
    10501 aaggggatta aaatggcaaa acaagtgttt caaacgactt ttgcgggtcg tgagttaatt
    10561 gtagagactg gtcaggttgc taagcaagca aatggatctg ttgtcgtacg ttacggtgag
    10621 tcaactgtct tgactgctgc cgttatgtct aagaaaatgg caactgggga tttcttccca
    10681 ctccaagtca actacgaaga aaaaatgtat gcggctggga agtttcctgg tggctttatg
    10741 aaacgtgaag gacgtccttc aacagatgcg accttgacag cgcgtttgat tgaccgtccg
    10801 attcgtccta tgtttgcgga aggtttccgt aatgaagtcc aagtcatcaa tacagtgctt
    10861 tcttatgatg aaaatgcatc tgcaccaatg gctgctatgt ttggttcatc tttggcactg
    10921 tctatttcag atattccatt tgacggacca attgctgggg tacaagtggg atatgtagat
    10981 ggccaaatca tcatcaaccc aagtcaagaa caagcagagc aatctcttct tgaattgaca
    11041 gtagctggaa ccaagcacgc tatcaacatg gtagagtctg gtgccaaaga attgtcagaa
    11101 gaaatcatgt tggaagcgct tcttaaaggg cacgaagctg tcaaagaatt gattgccttc
    11161 caagaagaaa tcgttgctgc tgtcggtaaa gaaaaagcag aagtggaatt gcttcacgtg
    11221 gatgctgaat tgcaagctga aatcattgca gcctacaaca gtgacctcca aaaggcagtt
    11281 caagtagaag agaaattggc ccgtgaagct gcaactcaag cagtgaaaga ccaagtgact
    11341 gccgtttacg aagaaaaata tgcggaccac gaagaatttg accgtattat gcgtgatgtg
    11401 gctgaaatct tggaacaaat ggaacacgca gaagtgcgac gtttaattac agaagacaag
    11461 gtgcgtcctg atggtcgtaa ggtcgatgaa atccgtcctt tggatgcggt tgttgacttc
    11521 cttcctcgtg tacatggttc aggtctcttt actcgtgggc aaactcaagc tctttcagtc
    11581 ttgaccttgg ctccgatggg agaaactcaa atcattgatg gtttggatcc agagtacaag
    11641 aaacgcttta tgcaccacta taacttccct caatattctg taggggaaac aggtcgttac
    11701 ggtgcgccag gtcgtcgtga aatcggtcac ggtgctcttg gtgagcgtgc tcttgctcaa
    11761 gtcttgccaa gcttggaaga attcccatac gctatccgtc tagtagcaga agttttggaa
    11821 tcaaacggtt cttcatctca agcttctatc tgtgcgggaa ctcttgccct tatggctggt
    11881 ggtgtgccaa tcaaggcgcc agtagctggt attgctatgg gacttatctc agatggaaat
    11941 aactacacag tattgacaga tatccaaggt ttggaagatc actttggaga tatggacttc
    12001 aaggttgcag gtactcgtga tgggattaca gcccttcaaa tggatatcaa gattcaaggg
    12061 attactgcag aaatcttgac ggaggctctt gctcaagcca agaaagcgcg ttttgaaatc
    12121 cttgatgtca ttgaagcaac cattccagaa gttcgtccag aattggctcc aactgctccg
    12181 aaaattgata cgatcaagat tgatgtagac aagattaaga ttgtcatcgg taagggtgga
    12241 gaaaccatcg acaagattat tgctgaaaca ggtgttaaga ttgatataga cgaagaagga
    12301 aatgtgtcta tctactctag tgaccaagat gctattaacc gtgccaaaga aattattgct
    12361 ggtttggttc gtgaagccaa agtggatgaa gtttaccgtg ctaaagtcgt tcgtatcgag
    12421 aaatttggtg cctttgttaa cctctttgat aagacagatg cccttgttca tatctctgag
    12481 atggcttgga ctcgtaccaa tcgtgtagag gatttggtag aaatcgggga tgaagttgat
    12541 gttaaggtta tcaagattga tgaaaaaggc cgtatcgatg cttctatgaa ggctcttcta
    12601 cctcgtccgc caaaacctga gcatgatgaa aaaggtgaaa agtctgagcg ccctcaccgc
    12661 ccacgtcatc acaaggatca caaacctaag aaagaattta cagaaacacc aaaagattca
    12721 gaataagaaa aggagaaatg tatggggtgg tggcgcgaaa ccattgatat cgtaaaagaa
    12781 aatgatccag cggcccgcac cactttggag gttttgctga cttatccagg tgtcaaggcc
    12841 ttggcggccc accgtctctc gcattttctc tggaagcaca gcttcaaact cttagctcgt
    12901 atgcacagtc agttttggcg cttttggact cagattgaga ttcatccagg agcccagatt
    12961 gattcaggtg tctttattga ccatggttct ggtctggtga ttggagagac agcgattgtt
    13021 gaaaaaggcg ttcttctcta tcacggagtg actcttgggg gaacagggaa agactgtggc
    13081 aaacgccatc cgactgtacg aaagggagcc ctcatatcag cccatgccca agttatcgga
    13141 cctgtggaaa ttggtgaaaa tgccaaggtc ggtgctgcag cagttgtcgt agcagatgta
    13201 cctagtgatg tgacggttgt cggtattccg gccaagattg tccgtcttca tggtaagaaa
    13261 gatgagcctg ttattcacgc agtcgaagaa aaacgagagt attatgtcaa taaactcgag
    13321 caggctaaag atgccagtca cagatcgtct ggtttgtaga ggatacctat atgattcaag
    13381 caagaaacaa gttaagccaa gaggagctat ctgaggcgaa aaaagtaatt aactgttgcc
    13441 aaaactatga cggtacctat cgcgatccct atctctctaa catgcttaat tttgacccaa
    13501 acatgcccgc ctttttcctt tattatgaaa aaggcaaact tgttggttta ttaactgtct
    13561 atgcagatga ccaagatgta gaagtgacga tactggttca tccaggtcat cgccgtcagg
    13621 ggattgcgcg tgcattgttt actagttttg agagagaaac agcttctttc cctattcggt
    13681 cagtcacttt tcagacagaa cgtatttttt tagagaatca tcctgacttt gccagtaact
    13741 ggggactaat cgaggatgaa gagacagaaa cttggttagg taaggataga agaccttatc
    13801 cgttagcaaa tgtttcccat cttgaaattt tgttagcaga tagttcgtat caggatcaaa
    13861 ttagtcaatt aaaatttcag gcattttcag aggaacatga atcgagagaa gttgtggata
    13921 gatatgtcgc tgaagctctg aaagatccag aaagccgact atatatttta ttaaaagctg
    13981 gtcaggttat tgggacttgc acggttgatt tatcgagcga tacgaattac ttctacggtt
    14041 tagcaatatc agaacctgaa cgtgggaaag gctatggaag ctacttagca aaatcccttg
    14101 tcaaccaact aattgagcaa aatgataagg aatttcagat tgcagtggaa gatagcaatg
    14161 taggtgccaa acgtttgtat gaaaaaattg gctttgtcaa acagacacag gtggtttatc
    14221 tgaatgagaa aggagcaagg gattccgaag tgtagagaca ttcggactga aatttacttg
    14281 agctcttagt ttagtgatga aactaacagt tcttggattt tagctttcct gattatgatt
    14341 tatgattaaa atctatgaca ccatgtctcg tgatttgcga gaatttgtcc cgattgagga
    14401 cggcaagatc aagatgtatg tttgtgggcc aacggtgtac aactatatcc acgtgggaaa
    14461 cgcccgttcg acggtagctt ttgatacgat tcgtcgctat tttgagtacc gtgggtataa
    14521 ggttgcctat atttccaatt ttacagatgt ggatgataag attatcaacc gtgccaggga
    14581 agaaggcatc acgcctcagg aggttgcgga taagtacatc gctgcctttc gtgaggatgt
    14641 gacggccttg ggcgtgaaac ctgcgactcg ccatccgcgt gtagtggagt ttatggcaga
    14701 catcatccgt tttgtggaag acttgatcga gaaaggcttt gcctatgaga gtcaagggga
    14761 tgtctatttc cgtgtagaaa aatcccacaa ctatgctaaa ttggctaata aaaccttgga
    14821 agatttggag ctaggtgctt caggtcgtac cgatgaagaa acggctcgta aggaaaatcc
    14881 tgtagacttt gctctatgga aatcttccaa accaggtgag atttcttggg atagtccttg
    14941 gggacctgga cgtccgggtt ggcatattga gtgttcagtc atgtcgacag agattttagg
    15001 tgataccatt gatatccacg gtggtggagc tgacctagag tttccacacc acaccaatga
    15061 aattgcccaa tctgaagcta aaacaggcaa ggcttttgct aactactgga tgcacaatgg
    15121 ctttgtcaat atcgacaatg tcaaaatgtc taagtccttg ggtaacttca ttacggtaca
    15181 cgatgccctt aaaactcttg atgggcaagt acttcgtttc ttctttgcga cccagcacta
    15241 ccgtaagcct atcaacttca cggaaaaggc agttcgcgat gccgagacca atctcaagta
    15301 tctgaagaat acttacgaac aaccatttac aggaaacgtg gatgcccaag agttacaaaa
    15361 ctttaaagat aagtttgtag cagctatgga tgaagatttt aatgctgcca acggtatcac
    15421 agttgtcttt gaaatggcca aatggatcaa ctcagggaac tatgatgcaa gtgtcaagca
    15481 agctcttgca gatatgttag aaatttttgg aattgtcttt gttgaggaag ttttggatgc
    15541 agagattgaa gacttgattc aaaaacgcca agaggcgcgt gccaatcgtg actttgcgac
    15601 agcagaccaa atccgtgacc aattggttac tcaaggaatt aagctccttg ataccaagga
    15661 tggagtgagg tggacacgtg attgatgtca atctcattaa cgggattgcg ctagcctttg
    15721 agggggatgc ggtgtattct atgtatattc gccgtcacct catcctcaaa ggtatgacca
    15781 aacccaataa actccatcaa gaagcaacca agtatgtgtc agccaaggct caggctcgcc
    15841 tgattgctct catgttggag gagcaggtcc taacggaaaa agaagaagaa atctacaaac
    15901 gtggccgcaa taccaatagc cacacaaagg ctaaaaatgc agatgtcgtg acttatcgta
    15961 tgtccacggg atttgaagcg gttatgggct atctccatat gactgagaat ctggaacgtc
    16021 ttgagagttt ggtttcatgg tgcatccaaa aagtggaggg ctagaacatg agggaaaaag
    16081 aactacaaga ctggtttcct gaggctcgga tttcagacca accagtagag aaagagggct
    16141 atctcacgct ccctttagct tctcagcagt ggattttgct ggaggaagct gggctcagcg
    16201 agcgtgaaaa gcagttggtt gcccttttga cccagcagga gcaggctcgt tcgctaaacc
    16261 cttggtattc ctatctggtt gaaggcaagg gacaggcacc gcaagttttt aaaaagattc
    16321 agttggttta ttgccatctt tcttattttc agcaggaaaa tctgacttct tggctagata
    16381 tgatgcggac tctttttccg aattgtcaga cagtgctaca ggtcggagct caggattatg
    16441 ttttcgtgct tcaacaagac aaatacactt ctgtaagaga tattttaagt gatacgattg
    16501 aagcggttga gtatgatttt ggacttcgtc tttctatcat gttgggtcag gtttggtctc
    16561 agacaggaca tcaagcccta tcagacttaa tcaaagctga gcgggatttg ttcaagacat
    16621 ggtggcgtca gggtcaccaa ggtgttcata ctttttctca gctctatctt tggagtatgg
    16681 gagaaagact cgtggacttg aagccaatta aagaatgtct acaccagatg attttggatc
    16741 aagatcagat tcaggaaatc attctctctc tttgggaaaa tagtgctgtt ctcactaaaa
    16801 cagcccagca actctatctg caccgcaatt ctctccaata caagattgat aaatgggaag
    16861 agttgacagg gcttcagttg aaagagttga ccgacctgac cttgtgttat caattgattt
    16921 taggttcttt gtcaactata gttggtttgt aaagaagtta atatttggag aagaggattg
    16981 ccatcttctc catttttatg tgcagagtta tagtggcttg atgctgggaa agtacactgt
    17041 gactgctaaa acattcctag aagctgcttt gatttcccta atctatttat gcaaatttta
    17101 tgttatttta ctataacagt tgttgctaag ccaaataata gtggggaagt tcatttagac
    17161 gtaagcattg aagataatca gggaggtagt gggtataatt tcagttctgt ttcaagtagc
    17221 tcacaaacag ctaaatatga aggaactggt tataataaca attcatcatt atatataacg
    17281 attgataaaa cgtctgatgc aacagctctt ttgaaattaa agttgaataa tgttgataat
    17341 caacctgcta ctgaagttcc tagttcagga attactgtaa aattaaatgc taaagataat
    17401 gctggaaact ggacaagtgc ttcgaataaa aaagaagtaa cagtaaaaat tgtttctgct
    17461 aaaccgacat atccagacaa aatcttagtg aaaaatcctg ataatataaa agatacagaa
    17521 aaaaatgcca ttattgaaaa attgaaagag gcaaataaaa atcatccagc aggagctcca
    17581 acctttgcta aaggtgaagg agagcatgca aatgatattg tagcaactta ttcagatggt
    17641 acaacttatt atgtaccgtt aaatgatgtg acaaaatatg cgaggtagtg gctgtaccac
    17701 tcacttattc acctccccgt gatttgtagt agtgataggc tttctcacta ttattataaa
    17761 acaaaataaa gatcacaaca ctttttcatt ctgtgttgtg ccttgagtga aacgaaagga
    17821 atgaattata aatatgaaaa gtatagtcac tagcatagat gagcgcttgc gtactcgcct
    17881 acgagtgatt atctggaagc aatggaagaa gaaatcgaga cgattatggg gattgcttaa
    17941 gttaggggtt cctaaatgga tagcagataa ggtatctggc tggggcgacc actatcaatt
    18001 atattaaaat gaaataagaa taggacgaat caatcaggga agtcaaatcg atttctaaca
    18061 atattttaga agtagaggtg tactattcta gtttcaatct actatagtag ctcagaagtc
//
LOCUS       DAVHIT010000037        17948 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVHIT010000037 DAVHIT010000000
VERSION     DAVHIT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.038"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            154..1608
                     /locus_tag="VVN16_001122"
     CDS             154..1608
                     /locus_tag="VVN16_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU5572523.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            1589..2554
                     /locus_tag="VVN16_001123"
     CDS             1589..2554
                     /locus_tag="VVN16_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5572524.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(2589..3863)
                     /locus_tag="VVN16_001124"
     CDS             complement(2589..3863)
                     /locus_tag="VVN16_001124"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU5572525.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQANSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3863..5035)
                     /locus_tag="VVN16_001125"
     CDS             complement(3863..5035)
                     /locus_tag="VVN16_001125"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU5572526.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(5166..5414)
                     /locus_tag="VVN16_001126"
     CDS             complement(5166..5414)
                     /locus_tag="VVN16_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572527.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QEFVDEMGRTYSGNLILQELITVNEAYKATYIDELSSN"
     gene            complement(5482..6107)
                     /locus_tag="VVN16_001127"
                     /pseudo
     CDS             complement(5482..6107)
                     /locus_tag="VVN16_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6169..6618)
                     /locus_tag="VVN16_001128"
     CDS             complement(6169..6618)
                     /locus_tag="VVN16_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU5572528.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(6620..7378)
                     /locus_tag="VVN16_001129"
     CDS             complement(6620..7378)
                     /locus_tag="VVN16_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU5572529.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(7493..9472)
                     /gene="pknB"
                     /locus_tag="VVN16_001130"
     CDS             complement(7493..9472)
                     /gene="pknB"
                     /locus_tag="VVN16_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU5572530.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENSSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(9469..10209)
                     /locus_tag="VVN16_001131"
     CDS             complement(9469..10209)
                     /locus_tag="VVN16_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU5572531.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(10224..11537)
                     /gene="rsmB"
                     /locus_tag="VVN16_001132"
     CDS             complement(10224..11537)
                     /gene="rsmB"
                     /locus_tag="VVN16_001132"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU5572532.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(11530..12465)
                     /locus_tag="VVN16_001133"
     CDS             complement(11530..12465)
                     /locus_tag="VVN16_001133"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5572533.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(12478..14874)
                     /locus_tag="VVN16_001134"
     CDS             complement(12478..14874)
                     /locus_tag="VVN16_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5572534.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVFGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(14940..15254)
                     /locus_tag="VVN16_001135"
     CDS             complement(14940..15254)
                     /locus_tag="VVN16_001135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU5572535.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(15279..15905)
                     /gene="gmk"
                     /locus_tag="VVN16_001136"
     CDS             complement(15279..15905)
                     /gene="gmk"
                     /locus_tag="VVN16_001136"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5572536.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(16035..17648)
                     /locus_tag="VVN16_001137"
     CDS             complement(16035..17648)
                     /locus_tag="VVN16_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5572537.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         4892 a         4214 c         3566 g         5276 t
ORIGIN      
        1 ttatctttca cattttcgta aaaatttgat tgatttcctg tatttatttt atgataatcg
       61 tttgacagtt ttttctcttt tttaatataa aaggcttgca ttttttatcg aaaacagtta
      121 ctattaaaag tgataagatt tttggaggaa tgaatggaat ggacaactga gcgtcgttac
      181 agactttatc aagattggac gcaagaagaa attcaacata taaaggaaaa tatggcacaa
      241 tctccatggc atactcatta ccatgttgag ccaaaaacag gacttctcaa cgacccaaat
      301 ggcttttctt actttgatgg caagtggatc ctcttttacc agaattttcc ttttggtgca
      361 gcccacggtt taaaatcttg ggcacagcta gaaagtgatg atttgattca ctttaaagaa
      421 actggaatca aagttttacc agatactcca ttagatagcc acggtgccta ctctggttct
      481 gccatgcaat ttggcgataa cttattccta ttttatacag gaaatgttcg cgataaaaac
      541 tggatccgtc acccatacca gatcggtgct ttgatggaca aggagggtaa gattacaaag
      601 attgacaaga tcttgattga ccagccagca gactctactg accacttccg cgatccacaa
      661 atttttaact ttcagggtca atattatgcc attgtcggcg gacaagactt ggagaaaaaa
      721 ggtttcgttc gtctctacaa ggctgtcaat aacgactaca caaactggca agcagttggc
      781 gaccttgact ttgctaacga ccgtactgcc tacatgatgg aatgtcctaa tttggtcttt
      841 gtagaggaac aacctgtcct tctctactgt ccacaaggat tggataagaa agttctagac
      901 tacgataata tctttccaaa tatgtataag atcggggctt cctttgaccc taaaaatgcc
      961 aaaatggtag atgtgtctca acttcaaaac atggattacg gtttcgaagc ctatgcaact
     1021 caagccttca acgctcctga tgggcgtgct ctagcagtta gctggcttgg tttgccagat
     1081 gtttcttacc catctgaccg ttttgaccac caaggaacct tctctttggt caaggaactc
     1141 actatcaaag acgacaagct ctaccagtat ccagtcgctg ctattaagga ccttcgtgct
     1201 tctgaagaag ccttctcaaa ccgttcccaa accaagaaca cttacgaact tgaactcaac
     1261 ttggaagcta atagccagag cgagattgtc ttacttgctg ataaagaagg taagggactt
     1321 tcaatcaact ttgaccttgt aaacggtcaa gtaacagtgg atcgtagcca ggctggagaa
     1381 cagtatgccc aagaatttgg gacaactcgt tcttgcccta tcgagaatca ggctactact
     1441 gctacaatct tcatcgataa ctctgtcttt gaaattttca tcaataaagg agaaaaagta
     1501 ttttctggtc gtgtcttccc acatgcggac caaaatggta tcctgattaa atctggaaac
     1561 ccaactggaa cttactatga attagattat ggtcgcaaaa ctaactgatg tcgccaaact
     1621 tgcaggcgtc agtcctacta ccgtttctcg ggttatcaat aaaaaagggt atctatctga
     1681 gaaaaccatc caaaaagtca atgaagccat gcgagaattg ggctataaac ccaacaacct
     1741 ggctcgtagt ctgcaaggaa aatcagctaa gttaatcggc ttgattttcc ccaatatttc
     1801 caatgttttc tatgcagaat tgattgataa attggaacac caactcttca aaaatggtta
     1861 caagaccatc atctgcaaca gtgaacatga ttctgagaag gaacgcgaat acatcgaaat
     1921 gttggaagcc aatcaggtgg acggcatcat ttctggtagt cacaacctag gaatcgaaga
     1981 ctacaatcgt gtgacagcgc cgattatttc ctttgaccga aacctatcgc cagacatccc
     2041 tgtcgtctcc tctgacaact atgctggtgg ggttcttgct gcccaaacct tggtcaagac
     2101 aggtgcccag tctatcatca tgattacagg gaatgacaat tccaattcgc caaccggact
     2161 gcgccacgct ggttttgcat ccgtactccc aaaagctcct attatcaatg tttccagtga
     2221 cttttctccc gtcagaaaag aaatggaaat caagaatatc ttgacccggg aaaaaccaga
     2281 tgccattttt gcttcggatg atttgacagc tattctggtc attaaaatcg ctcaagaatt
     2341 gggcatttct gtcccaaaag agctcaaggt catcggctat gatgggacct actttatcga
     2401 aaattactac cctcaattgg ctactatcaa gcaacctttg gaagagattg cttgtctcac
     2461 tattgatctt ctcttgcaaa agattgaagg caaggaagtc gccacaactg gttacttctt
     2521 accagttacg ctattaccag gaaaaagtat ttaaacacaa gaaaactcag accgattcgt
     2581 ctgagttttt atgatcttaa attttcgaga tagcgctggg ctgtctctag gttaaaggtt
     2641 ttatctgaga tgaggcgctc tactagggga gcaacttcag attcactagc cccagctagg
     2701 agagctaggg agttggcctg tagtttcatg tggccttgct ggatacccgt acttaccaag
     2761 gctttgaggg ctgcaaaatt ttgggcaaga ccgatggaca cgataatctg ggctaattct
     2821 ctggcagaag gatttcctag tagatcatga ctgagagcta cacgtgggtt gaggccgata
     2881 gagccaccct tagtcgctac aggcatgggc agggtcatct caccgaccaa ttcttctctt
     2941 tcaaggtcca gcgtccagca actaagacct tgatagcgtc catctcgact ggcaaaggca
     3001 tgggccccag cttcgatggc acgccagtca ttaccagtgg caatcaaaat cgcatcaata
     3061 ccattaaaaa ttcctttatt atgagtagca gctcggtaag gatcagcctg cgcaaactga
     3121 ctagccaacg caattttctc cgcaatctct cgtccttgat ccttttggcg gctcaagtag
     3181 cgaaaggcca tgcgacagct tgcagtcacc agagaatcgg tcgcgtagtt ggacaggatt
     3241 cccatgagac tctgtccctg actgagttct tctaagactg gtttcaaggc ttccagcatg
     3301 gtgttgagca tattggcacc catggcttcc tgggtatcaa catgaatata aacaacgaga
     3361 aagtctggtt cgccttttat ctgctcgaca tgcaggtcac gcgccccacc cccacgttta
     3421 acgatagaag gataggcttg attggcaagc tccaagagct ccgctttctt gctggcaatc
     3481 ttctcttgcg ctagtttagg attagcaact tgataaaggg ctacctgccc aatcatctgg
     3541 cgctgatgga cttgtgcagt aaaaccacct gcacgcttga tgattttgct ggcatagctg
     3601 gccgctgcaa ccacagaggg ttcttctgtc acatagggaa cggtgtattc ctgaccgttg
     3661 acaagtacct ccggaaccag cgaataaggc agagaaaaag ttcccactac attctcactc
     3721 agctggtctg ccacagtcac gctcatctgt tcatccttct ccagactagc ttgtctctca
     3781 ggactaagga gcgcctgagc ttttaacagc tcgaggcgct cttggtatga ttttttagaa
     3841 aatccattcc aacttatctt cattattttt caaccttgct ataacggcgt tggtggtcga
     3901 gaatttcaac caaggcaaaa tcttgatttt catagccagc aaactgggca gagtttgttt
     3961 catccaagtt tacttcctca aaaaagacct tttcatagtc tgcaacggat aaggcagttc
     4021 gttggttgag cttgttcaag cggtctttat ccaaataagc ttcatatcct tcaaccaatt
     4081 caccactgaa gaactcagcc acagctccac ttccgtaact ataaagggcg attttatccc
     4141 cagctttcaa gctatctgta ttttccaaga gagacaaaag tccaaggaaa agtgaacctg
     4201 tgtagatatt ccccaccttt tgactgtaga gaatagactg gtcaaaatgc ttttgtaaga
     4261 ggtctttttt ctcttgaggc aggctcttat ccatgatttt tttcaagcct tttagcgcta
     4321 atttaggata aggcaagtgg aaacaaacag ccgcaaaatc atccagagta agctggtagc
     4381 gtttttgata ttcaagccaa gtcgttttca aactatccaa gtattgttgg gtagaataaa
     4441 caccatttac ataaggagtt gtcgagtaat ttggtcgcca gaaatccatg atgtcacggg
     4501 tctgagctac attgtcatta ttaaaggcca tcatgcgtgg attttgtgta atcaacatag
     4561 ctacacttcc agcaccttga gttggttctc ctggagtttc aataccgtat ttggcaatat
     4621 cactggcaat gaccaagacc ttggactccg gagaattttc cacatgcaat ttggcataat
     4681 gaagggcagc agtcgctcca taacaggctt ctttaatctc gaaactacga gcaaagggct
     4741 ggatgcccag caagccatgc acaaagacgg ccgcagcctt actctggtca attcctgact
     4801 cggtagccac aatgaccatg tcaacttctt gtctttcttg ctcagttaaa atagagtcac
     4861 tagcactggc cgccaaggtc acaatgtcct cagttagggg cgcaatactc aattccttga
     4921 gtaagagtcc tttacttaat ttttcagggt caattcccct cgcttctgct aagtcttgta
     4981 atttcaagac atattgactg gtcgcaaaac caatcttatc aataccgatt gtcatattta
     5041 cctctgtttt atcattcatg taaaaaatcg ttctatacta ttttatcaca aatggcagta
     5101 aaagagagaa aaaagacttg attcaccaaa tcaagcctct tattggtcat cattttaaag
     5161 aatgattagt tgctagaaag ttcatcgata taagtagctt tataagcttc attcacagtt
     5221 atcagctcct ggaggatcaa atttcctgag taagtccttc ccatctcatc tacaaattct
     5281 tgataaaact gactggtcgg aatttctctg acatccttat caaatgtctt gtcaagtgtt
     5341 ttactaacct tctcagcaat caattgatgc tcttgccatc cactttgaaa ctctgagccc
     5401 gaactagaaa ccatgactgg gataaacaac aaggtcagta gacttacaga caataaggaa
     5461 agtagtagac ttcctgcaaa actagaatcc tagttcatga ttgataatgc cagcaatcaa
     5521 attcattcgt aatccgaagc gtttacgatg atttcgatag gttgttgaaa acattttaaa
     5581 cgtttttact ttggcaagaa tgttctcaac cttgcttcta tccttagata gcgcatggtt
     5641 ataggcttta tcttcaactg ttagcggctt gagtttgctg gatttacgtg gagtttgtac
     5701 ttgaggatat atcttcatga gcccttgata accactgtca gccaagattt taccagcttg
     5761 tccgatattt ctgcaactca ttttgaacaa cttcatatca tgacaatagt tcacagtgat
     5821 atccaaagaa acaattctcc cttgacttgt gacaatcgct tgagccttca tagcgtggca
     5881 tttcttttta ccagaatcat tcgctaattc tttttttagg tcgattgatt tgtacttccg
     5941 tcgtatcaat cattaccgtg tcctcagagc tgagaggagt tcttgaaatc gtaacaccac
     6001 ctttgaacaa gagttacttc aacccattag ctccgacgga ttaagttgct ttcgtgaata
     6061 ccaaaatcag ccgcaatttc ttcataagtg cggtattctc gcacatatag aaagcgttgt
     6121 taatttattt atctcatttt tcagaaaatt cttttatttc tgtaaagtct acgatactcg
     6181 atgtgttttt atataatgat acagtctgag aatcactgtt ccgctagcca ttccaataga
     6241 gattaccaaa gccaacatga caaccaaggt cgcacttgcc agtgctttat tatagtcccc
     6301 tgtcacaaaa aaggcagttg ttcggtaaga gagataacct ggaaccagcg gtgccaaaat
     6361 ggccaagata aagaccacag caggtgtctt ataaagaata cttaaaatct ggctgacaca
     6421 agaaccaata atggctgcaa tgaaggtagc tacaatgaca ttggtcggtt ccttgagcaa
     6481 gagatagatt agccagacag tcatgcccaa aatccctcca ggtaagagca tagaccgttg
     6541 cacattgagt acgattaaaa aagtgataat ggcaagaaaa cttgctactg cttgtaataa
     6601 aaaggttgtt agtgtcatat tagttcatca ataccaaggc gacagaagtt cctgccccta
     6661 aagcgagggt aatgagcagg gattcaaaca tcttactcat accagagttt atgtggttgg
     6721 tcataatatc acggaccgca ttggtcaagg caatacctgg tacaaacggc atgaccgcac
     6781 cagctataat caaatctgcc gttgaaggaa aacctgtgta gcgagcccaa aactgggcaa
     6841 ttatcccaaa gacaaaggct ccagcaaagg ctgtcacaaa gggaattcgg ataaattttt
     6901 ccacatagag ggaaaaggca aaaccaaata aggtcgccac tcctgcccca agtgcgtcgt
     6961 agatatttcc gctaaacata actgaaaaga aaggagcact aaaggtcgca gccagagtta
     7021 cctgcaactt agtataggga aggggttgag cttgcaaggc cgtcaattgc ttaaaggctg
     7081 tttctaagtc aatctgcccc ccaactagct gacgagaaat ctggttcaca tcgcagactt
     7141 tttcgatgtt ataagaagag gaggtcacgc gcttcatgcg cgaaatattg gtattttcaa
     7201 tagagaaaaa gatagcggca ggcatggcaa ggacattgca atccacaatc ccctgcgaat
     7261 gcgcgattcg aatcatggta tcttctacac gatggatttc tgagccactt ttaaggagaa
     7321 tagtccccgc tagcataatc acatcaatga cggcatttaa ttcttttgat tcttccatgc
     7381 tttcctcctt ttatcaactc cctctattct atcacaaatc tggactcaaa aaaaatcttt
     7441 gccatgaaat catgacaaag attgattact cattttgatt atccatctgc ttttaaggag
     7501 tagctgaagt tgttttaggt ttgtagattg aaatcttgac tctagtctta tttaggtcta
     7561 ccttttcacc tgctctagga ctttgttcaa caaccatgcc ttctacacta cctgcaggcg
     7621 ctgtcgtcac ttctacaact tctatattag cttccttaat cccaacaatt tgaatcaaat
     7681 tgttcttagt aaactccaag ctggaaccaa tgtaactcgg catggcaaca cttgtaactt
     7741 ttttagctac tgtcaagaca atttgagtag gtttactcac atcataagtc gttccggcac
     7801 ctggactttg tttcataatc gttcctggtt cgctttcgct ggactcttct tcctctatct
     7861 taatcaaatt ctcaggaacc ttcttctgct tgagttctga gattacttct gtagagttcc
     7921 gtccaatata gttccctaat tgaatcgtcg tagctttttt agctactgtc aaaacaattt
     7981 gagttgcctt gctcaagtca taggtcgtac cttctggtag actttgcttc aggaccgttc
     8041 cagcctcact ttcattcgac tcttcttcct caattttaat caaattatct ggaacttttt
     8101 tctcttttaa ttccgcgata acatcagaag atttccggcc gacataatta ctaatttgga
     8161 aggattgttt gcctgatgag acaaccagat taattttcgt tccttctttt cgaccagttc
     8221 cagcgccagg atctgtacgg ataatccgcc cttcttccac cttttcacta gcctctgtct
     8281 tctcctcacc aatctcaaaa ttggcttttt tgagcgttgc cttggcctct gcaactgtct
     8341 gacctgccac atctggaatg gcaatggttg caggagttct ggatagtatc caaataagag
     8401 aagctgccac caatacaagg ctggccaaca aaatcaggta acgcatctta aatctatgtt
     8461 ttttcggtgc ttgtggttgg taagtttcct ctgtcacagc ctggcttggg tttttgattg
     8521 atttgtgttc tgtctgcgct tgaaccttag gaatagatgt caaggtactc tgagaaacct
     8581 tcggcaaggt cttggtatct gccttgctcg tttcatcaaa gattaactta ctttcatttc
     8641 tacgattgta ggacaagcta ctagacaagt ctacatacat ctctgaaacc gagcgatagc
     8701 gattggtcaa ctttttagca gttgccttga taataacatt ttctaaagcc tgaggtacag
     8761 atgaattttc tgcaataacg gacggcaggg gtttctggaa atgctggagg gcaatggtca
     8821 ccgcgctatc cccgtcataa gggatatggc ctgtcaacat ctcatagaaa ataatcccca
     8881 tggcatagat atcactctgc acagtcgcct tcgaaccacg cgcctgctct ggtgacaagt
     8941 aatgaactga gcccaacatc gagttagtct gggtcagact tgtctctgca aaggctacag
     9001 caatcccaaa gtctgtgacc ttggcagtcc catctggtgt caaaaggata ttttgaggtt
     9061 tcaagtccct gtgaacaatt cctcgagtat gggccaagcg catagccaag agaatttgtc
     9121 ccatgatacg gactgcttct tcattagaaa gaggataatg ttccttgata tagcgtttga
     9181 ggtctagtcc agcaacatac tccattgcaa gatactgttg accgtcttcc tcaccaatat
     9241 ctgttatccg aacgatatga ggatggtcta gatctgccat agctctcgct tcacgctgaa
     9301 aacgagctac agctatcggg tccgtctggt agttggtcct cagaaccttc actgccactt
     9361 cttccccatc taagattaag tctttggcta ggtagacatc cgccatgcct cctcgaccaa
     9421 tctgtttgac aatccgatag cgtccggcaa aaatcttgcc gatttggatc attctgcatc
     9481 ctcctcgttc atagaaacaa gggcaaccgt aatgttgtct aaacctcctg cattgttagc
     9541 aaaacgaaca agtgtctccg ttttatctgc taaaggaata tcactggtta caatatcacg
     9601 aatctcactg cctgaaatca tgttggtcaa gccgtcacta ttgagcaaaa gatagtcacc
     9661 tgactcaagg ataactgtcc caaaatcagg ctgaatttca tctttttgcc caatagactg
     9721 ggtgataata tttttttgcg gatgagcttc tgcctcttct ggtgtcaatt gaccagcctt
     9781 gagcaattca ttaaccaagg aatgatcgct cgtcaactga tggtattctt ctccacgaat
     9841 caagccgata cgcgaatcac caatatgagc atagatagcc tgattatcaa taatagcaag
     9901 gacttccaaa gtagttccca tgcctctgta agcttcatcc tgaccaagct ggtgaatctt
     9961 ttgattttca atttctaggt aatgggcgaa ccattcacgc acttcattga ctgtatcaat
    10021 ctgggtatca acccaagcta cacccaggtc tgtgaccgcc atttcactag cgatattccc
    10081 tgcgcgatga cctcccatcc catcagctaa aataatcatg gtacgtccag ctctattgac
    10141 atagtggttg acatagtctt ggttatttgt tcgtttctga ccaacatctg ttaataatga
    10201 aatttccatg tgtcagttcc ttcctaatcc gatatcttgc gaaattgact gatgaagaat
    10261 ccatcacttc catacaattc aggtgtaatg aggatacagc cgtctttcat gatatcctta
    10321 cattcatgtt ctagttttac ctgctcgaac tcgggatgac tctctaaaaa ggccttaacg
    10381 acttgaaaat tctcctctga gacgatagtg caggtgctat aagttattat accacctttg
    10441 cctagtattt gacaaacact acctaatatt tctaactgaa tttcctgcaa ggacgcgaaa
    10501 tctgccgttt ctttattgta tttgatatct ggttttcggc gcaaaagacc gattcctgaa
    10561 caaggagcat ccaccaaaat cttatcaaag gaatcctggt caaaaaactc atgcaccttt
    10621 ctggcatcca atttttgagt ttgaacccga tcttcaactc ccagacgttg ggcattttct
    10681 tgaattaaat ccaacttgtg gtcgtacaag tccagagcag taacctgacc tgtcgtaaga
    10741 taagaggcta tatgggctgt tttcccacct ggagccgcac aggcatcaag cactcgctca
    10801 tcaccttgta aatcaagcgt cggagcaacc agctgactgg actcgtcttg gatggtaatg
    10861 gctccatccg caaacaaatt atgccctgca aaatgcccct gctccttaac cagaccagtg
    10921 gttgctaaaa gggaatcatt cgcctccaac aaggcttgga tttcctcttt tcgacttagg
    10981 tctgttacac gaatactggc tttgtttcgc actaacaggc tttcaaagat ggcttttgct
    11041 ctctcctctc cgtattcttc cttgagtttg gcaactagcc aaactgggag agaataggca
    11101 atggagtcac gcttgttttt tcgcttgatg ctagcaatat ctggccagcc ttcacgcaag
    11161 atacggcgaa ggacagcgtt gaccaatttt tcactgcctt ttttacggag tttggccaat
    11221 tccactgctt cattgaccac agcatgatct ggaatcttgt ccaaatagcg gagttggtag
    11281 gcactcatga gaagaaggac atagagccag ctgtctaact ggtctctgtc ttcgataaag
    11341 tgggataggt accattccag agtcagttta cgggctaccg ttccatagac tagctcggtc
    11401 actaagccct tgtctgctgc agagagctga ctccccttga ggtgtttatt taaggcaata
    11461 tttgaatatg cttgattcac aaaaacatcc tctagcactg ctagagctaa acttctagcc
    11521 gtttctactt tagtcaccaa atcgttctcc tacagtcaat gtacgtccaa ctccattgag
    11581 gaaggaagca atgtccatct taggcttacc agctggctgc acttgtttga gggatagagc
    11641 cccttcagcc gttgcgacaa tcaattcttt cttgccgata gagagaatct cacctggatt
    11701 tccctgacct tctactggta gggcttcata aatcttaaag cggtcgccct taaggaaagt
    11761 atgggcaaca ggccaggggt tcattccacg aatttggtta aagagttgac gattggtttt
    11821 gttccagtcc agtttttctt cctctggctt tatatttgga gagaaggtaa cctgactcgt
    11881 atcctgcggt tcaggtttga tatcaccagc aatataggca ggcagagtgt ccaaaagcaa
    11941 atcacgacca actagcgcca atttttcaaa caaggtgcca acattgtcct catctgtgat
    12001 cggaatgctg cgacgagaaa tcatatctcc tgcatccatt tccttaacca tttccatgat
    12061 ggtcacacca gcttcctcat ccccttgaat caaggcataa tggataggcg caccaccacg
    12121 gtgtctagga aggagggagg catgaacgtt gacagcaaag tccatgctat caaggagttt
    12181 gcttgggaga aactgcccaa aagcagcagt cacaattcca tctgctccta gcttcataag
    12241 ttcttccatc tctggacttc cagataattt ttcaggttgg tagatagata gtcctgcttc
    12301 cttggcagcc tgcttgactg gggtttcttg gataactttt ttacgaccaa cagcacggtc
    12361 tggctgggtc acaacggcta gaatttcgta acggtcatct gtcaaaagtc cttttaagac
    12421 tgttgctgaa aagtcggggg tccccataaa gattagtttt gtcatatctt ctccttctta
    12481 taaaaattgc tgcggctcat ggtcaatgct gagacggagc tcactatttt cccgttcttg
    12541 agtcaaggcc aagacctggt tgagggtcgg ccccagctca tcttctaaac ggtatttaat
    12601 taaaatctgg taatgataga ggttgtgggt acgggcaatc ggttttggcg ttggccccag
    12661 aatgggactg gtctctgaca agcctgaccg caaaatgttc atgacttcat aggcacgttt
    12721 gaaaacctct tcttctttct tgtgagaaag ggtaatacca atcgtgaaat agtaaggtgg
    12781 atagccgagt tgtcgtctga ttcccatttc ataggcataa aagccttcgt aatcttgatc
    12841 cttggcaaat cgaatagcat agtgctgcga attgtaggac tgtatcaaga cttgacctgc
    12901 tttttcagcc cgacctgctc gacctgccac ctgagtcaag agctggaagg ttctctcaga
    12961 agaacggaaa tcaggcagat tcaaggccgt atccgcattt agaactccga ctagggtaac
    13021 attgggaaaa tccaaaccct ttgcaatcat ctgagtacca agtaaaatat ccgcttcccc
    13081 tcgcccaaac tggtcaagca aggcttggtg actgcctttc tttcgagtcg tatccacatc
    13141 catcctcaaa atgcgagctt ggggaaagag ttctgctagc tcatcataag ccttctgagt
    13201 tcccgtccca tagtaacgaa tactgtggct cttacagtta ggacagacct gaggaatatc
    13261 cttcgagaaa ccacaataat ggcagttcat agtcttggta tccatatgca aggtcagaga
    13321 aatatcgcag ttgggacaag tatccaccgt cccacactcc cgacacatga caaagctaga
    13381 ataaccacgg cgattgagca tgagaaccac ctgctctttt ttaaccagac ggtcttggat
    13441 agcctctagc aaaggaggcg taaagtttga cgtctcattt tgtccgatat agtctcgaaa
    13501 gtcaatcact tgaacctcag ggattgtagc caaaggattg gcacgttggg ttagacgtaa
    13561 gtgttgatag acgcctttgc cagcacgtgc ccggctctct aagctcggcg ttgcagatcc
    13621 aagtaccaga gttgcttgat tatactgagc ccgtaaaata gctacctctc tggcatggta
    13681 acggggattg ctgtcctgct tataagccgc ttcatgctct tcatcaataa tcatgacacc
    13741 cagatttttc agaggagcaa agatagcaga tctggcacca acaacaactt gggcatcgcc
    13801 acgctccacc ttgcgccatt catcatactt ttcgccattg gataatcctg agtgaagaat
    13861 ggctaccttg tccccaaaac gtgctataaa acgctcggtc atctgaggag tcaaggaaat
    13921 ctcaggtacc agcaaaatag ctgtcttgcc cttatccagg gcaccttgga taatctgcaa
    13981 gtaaacctcg gtcttcccac ttcctgtaat cccttgaagt agaaagggag gttgagaact
    14041 gccaatagaa ctcacaaccg catcacgcgc ctgtctttgt tctggattta actccaaagg
    14101 tctacttgct tcaattcctt caaaataagc agccgagcgt cgaacttcct tttggactat
    14161 ggtaacagca ccttgatcca caaagaagtt gacttgctct cgcgagtagg actctaacaa
    14221 gctagccaag gaagcgctct ctggatgaga cagcagataa tctctcagtt ccaacttttt
    14281 cttggcacgt gtagaaatct caacaccttc taattgagca tggtcaacct cataccaaga
    14341 ctgggtcttg accttctttt gatcgactgc ctgatattcc agaccaagca ggccttttct
    14401 agtcaaacgc atcatttcag cttgcttacc taaatccaga gaagaaaagg ctagcgaatc
    14461 ttctgaacca aacaggcgca ctcgttcttc ctgactcaag ccttccagag gatagagaat
    14521 cttgtcatag ctagaattca gaaatcccgg aagcatggcc ttgaggatag agattttgta
    14581 ggagaagaca gatttgcgta actcctcagc cagccagagt tgttctggcg tgagaacagg
    14641 agaaaaatcc agcacctctg caatatcttt taaatcttgc tccatctctt ctccatctga
    14701 ttgggactcc aaaccaaaaa caatcccttg aatcaggcga ttacccttac caaaaggcac
    14761 atgaacccgc atcccaactt ccagcattcc ctcaaattcc tccggaatcc tgtaactata
    14821 gggctggtcc gtctgcatca agggcacatc tacgataatc ttagctaggg ccatcttctc
    14881 acctcctcct tgtcagtaca ttcttgcaat agaaaaaata agattgagtc cccccaacct
    14941 taaatttttt caccatcttc tttttcttta gcaatttgct ctttgatttt cttttcttct
    15001 tcttctttgc ggcgtttttc ttcttcgata cggcgacgca ctgcttcacg ttttccttct
    15061 ggatctgggt gaattgtaac gtttcctgat tcgatttctt ctaaagcgcg aagagttgat
    15121 ttttcagact tgaaaccttg agttgctggg gcacctgctt ccaattcgtg ggcacgtttt
    15181 gcttccaaga ttacgagtga atattttgaa ggaaccttgt cgagcaaggt atcaatagag
    15241 ggttttaaca tcatttgctt gtacctattt tctaaatttt atcgggtagt tggagatttt
    15301 ggtaacatct cctgatagtg accaatgaca cgatccacac agaagtgttc tgcttcaatc
    15361 acacatttga cacgttcagc agctaggggc acctgatcgt tgacaatcgc ataatcatac
    15421 tcacgcatga gggcaatttc ttccttggcc ttttcgattc gttgggcaat cacttctgca
    15481 ctatctgttc cacgacctac caagcgatct tgcaattcat ccaaatctgg tggtgtcagg
    15541 aagataaaga cagcatctgg aacctttttc ttgacctgaa gagcaccctg aacttcaatt
    15601 tcaaggaaaa catcgattcc cttgtccaag gtttcattga cataggtcag aggagttcca
    15661 tagtagttgc cgacatattc tgcgtattcc aacatctgtc cttgacgaat cagctcttca
    15721 aattcttcac gagtacggaa gaaatagtca acaccgtcca cttctccagg acgttgtgcg
    15781 cgtgtcgtca tcgatacaga gtattgaaat tggttttcag aactctcaaa aatctctctt
    15841 ctaaccgttc cttttccaac ccctgaagga ccagaaaaaa cgattagtaa gcctcggtct
    15901 gccattgtgt ctccttttag tcaatctgtg aaataacatt tctctagaat aatggcaaaa
    15961 agccagatta tcctttacag tctttctatc tagtgtaaca aaaaagcagt aatttttcaa
    16021 ctgctctttc ttatttattt agcataatct actgcacgaa gctcgcgaat cacggttacc
    16081 ttgatatttc ctggataatc gagattgttt tcaattttct tacgaacttt gtgagccaag
    16141 attgtgactt tgtcgtcctt gatttttcct ggattgacca tgatacgaat ttcacgtcct
    16201 gcttgaaggg caaagctagt ttgcactcct tcaaagccgt tagcaatttc ttccaaatca
    16261 tggagacgct tgatgtagct ttcaagagac tcactacgag cacctggacg ggctgcgctc
    16321 aaggcatctg ctgcagcgac gataactgct atcacgctct cagcttcaac atctccgtgg
    16381 tgactagcaa tcgtattcac cacaactggg ggttccttgt acttacgggc caattccata
    16441 ccgatttcaa cgtggctacc ttcaacctca tggtcaatgg ctttcccgat atcgtgaagg
    16501 aatccagcac gacgggcaag agccgcattt tcaccaagtt cgctcgccat gataccagcc
    16561 aacttagcaa cctcaatcga atggcgcaaa acattttgtc catatgaagt acggaactgc
    16621 aaacgtccca taatcttcat caagtctgga tgaaggtttg gcgcaccaat ttcataagca
    16681 gcagcctcac cgtattcacg aatcttattg tcaatctctt gacggttttt ctcaaccaac
    16741 tcttcgatac gagctggatg tatacgacca tctttgagca acatttccat agtcatacgg
    16801 gcaatctcac gacgaatcgg atcaaatcct gacaaggtca ccacttctgg tgtatcgtcg
    16861 ataatcacat cgacccctgt caaactttca aaggtacgaa tgttacgacc ttcacgacca
    16921 ataatgcgtc ccttcatagt atcgtctggc agatgaaccg ttgagtttgt tgactccgct
    16981 acatattcac cagcgatacg ttgcatagct tgaaccaaga tgtccttggc cattttgtca
    17041 gagcgttcct tgacctcttg ctcagcttcg cgaatgcgac tggcaatctc cctggtcaag
    17101 ttttcctctg tctgagccaa gataatatct cgtgcttctg cctgagacag agcaccaata
    17161 cgctctagtt ctgcttcttt ttgtctttcg acttcctcta attgctcttc acgcgcatca
    17221 aggtttttcg ctctatcaga aatactttgt tctttttgtt caagtgtttg ttctttactc
    17281 gtcaaattgt cgtccttacg gtcaaggcta gtagctctct ctgtcaaacg actttctatt
    17341 tgtttgagtt cttgacgttc tgatttgaat tcagcgtcca cttcttcacg gtattttctg
    17401 gcttcttctt tggcctccaa tagtgcttct tttttaagag acttgctttc acgtttggct
    17461 tcattaacaa gtaaatccgc ttcacgctca gcttgtccac gtaaattagt tgcttcttgt
    17521 tcagcattta aaagcatcaa ctctgcagct tcctgagatg atttcatctt agctgagatg
    17581 ctgacatatc caatgactaa accaatgatg acggcaaaaa cagcaatcgc aagcgacatg
    17641 atttccatgt ttttacctca ttttattgtt attccgaatg acatacattc ttttacattc
    17701 taccataaaa aagtgatttt cacaaaccta aaatagaata tgttttgggg aatttagtct
    17761 gaattttagg attacacttc cttggttttt taaggagata agtcagtgtc gctatcaaaa
    17821 agttccaccg aatttctaca actatggatt ttctattgtt gtgccaaata gtgttccact
    17881 agattcaaat attttgcttt caagtctttg aaaattcact ttaggaaatt acagcttatc
    17941 ttcaagat
//
LOCUS       DAVHIT010000038        17480 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVHIT010000038 DAVHIT010000000
VERSION     DAVHIT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.039"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            24..498
                     /locus_tag="VVN16_001138"
                     /pseudo
     CDS             24..498
                     /locus_tag="VVN16_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
     gene            780..1027
                     /locus_tag="VVN16_001139"
                     /pseudo
     CDS             780..1027
                     /locus_tag="VVN16_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            1132..1434
                     /locus_tag="VVN16_001140"
                     /pseudo
     CDS             1132..1434
                     /locus_tag="VVN16_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1590..2819
                     /locus_tag="VVN16_001141"
     CDS             1590..2819
                     /locus_tag="VVN16_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5572538.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2839..5535
                     /gene="ppc"
                     /locus_tag="VVN16_001142"
     CDS             2839..5535
                     /gene="ppc"
                     /locus_tag="VVN16_001142"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU5572539.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITHKKSDTNTPNRYEAIMDQVVDRS
                     YDVYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5618..5835
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5948..6952
                     /locus_tag="VVN16_001143"
     CDS             5948..6952
                     /locus_tag="VVN16_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5572540.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7021..7887
                     /locus_tag="VVN16_001144"
     CDS             7021..7887
                     /locus_tag="VVN16_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572541.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7884..8642
                     /locus_tag="VVN16_001145"
     CDS             7884..8642
                     /locus_tag="VVN16_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572542.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            8732..10492
                     /gene="dnaG"
                     /locus_tag="VVN16_001146"
     CDS             8732..10492
                     /gene="dnaG"
                     /locus_tag="VVN16_001146"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5572543.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPYQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10495..11604
                     /gene="rpoD"
                     /locus_tag="VVN16_001147"
     CDS             10495..11604
                     /gene="rpoD"
                     /locus_tag="VVN16_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU5572544.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11618..11947
                     /locus_tag="VVN16_001148"
     CDS             11618..11947
                     /locus_tag="VVN16_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5572545.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12062..13105
                     /gene="cpoA"
                     /locus_tag="VVN16_001149"
     CDS             12062..13105
                     /gene="cpoA"
                     /locus_tag="VVN16_001149"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU5572546.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13121..14446
                     /locus_tag="VVN16_001150"
     CDS             13121..14446
                     /locus_tag="VVN16_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5572547.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14477..14653
                     /locus_tag="VVN16_001151"
     CDS             14477..14653
                     /locus_tag="VVN16_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5572548.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14712..14846
                     /locus_tag="VVN16_001152"
     CDS             14712..14846
                     /locus_tag="VVN16_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU5572549.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14910..16220
                     /gene="obgE"
                     /locus_tag="VVN16_001153"
     CDS             14910..16220
                     /gene="obgE"
                     /locus_tag="VVN16_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU5572550.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16230..16388
                     /locus_tag="VVN16_001154"
     CDS             16230..16388
                     /locus_tag="VVN16_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572551.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(16440..17480)
                     /locus_tag="VVN16_001155"
                     /pseudo
     CDS             complement(16440..17480)
                     /locus_tag="VVN16_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5400 a         3000 c         3896 g         5184 t
ORIGIN      
        1 ttcgagaaaa ttgggaaccg tacatgaaaa agtacacgga agtttttcaa agtcaatttc
       61 ctaatctaaa cattgattac ttgctcctgg acactgagca gattgatctt gatagctatc
      121 tagatgctga cataattatc atcggtggtg gaaatacgga aaaatatatt gctaacttat
      181 gttaatcagg agttcaaaag ttatatctat catatgctta ataaagaggc aaaaattata
      241 ggattttctg caggagccct actattagga gaaaaagtct atgtctcacc taatgataat
      301 tcagatcatc agataaagat aaaaaatgga ttaggactct ttagtcagtt tttaattagt
      361 gtccattatg attcctggaa tgataaagca aataaggata gagctgaaga actcgttaat
      421 gttcccataa ttccactaaa tgatcattct tgtcttgtat tggataaact tggaaacatc
      481 atagagaaaa ttgactaact ttcataatgt aaaaatggat ctaggaaata aaagtgtaga
      541 cgaactggat aggttactag tgaaaactgc aaaaagtgtt gtaaagtgaa tcggagtgtg
      601 cttgatggat tttagtagta agatagccat aaataaaggc tggtcagatg ataaaaaata
      661 ttgtgtgaca gatcaaaatc agtaaaaata tttcttgcgt gtttctgata agtcgaagtt
      721 agattataaa aaaatttgaa tttgatatga tggagaaagt agcttctctt ggagttccga
      781 tgtgtaaacc aattagcatt gaactctgcg atgacgaagt acattcttta cacgaatgga
      841 tagacggaag agatgcaata gatagcattt tagcttattc agaaaaccaa caatacacat
      901 atggtgtaga agcaggaaag atacttagaa agattcatac aattcctgct acagaagttt
      961 gtgaagattg ggaaattttt ttaatctaaa aattgatgat aaaatctcca acgaaatgat
     1021 atggtaagtc cgaaacgctc agtgtgtaag cttgttgcta aaagcgtaaa aaccttggaa
     1081 cgaaaggaat aatagacttc ctgcgaaaca aaaatacaat acaataaaac tatggatgat
     1141 gaagcaagta aacaattaag cgatagccgt ttcaagatcc ttgtaggtgt tcagcgcacg
     1201 acttttgaag agatgttagc tgtgttaaaa acagcttatc aacgtaaacg cgcaaaaggt
     1261 ggacgaaaaa gcaaattaag cctagacgat ctccttatgg taactattca atacatgcga
     1321 gaatagagca cttatgaaca aattgcggct gattttggca ttcacgaaag caacttaatc
     1381 cgtcggagtc aatgggttga agcaactctt attcaaaatg gttttacgat ttcaaattct
     1441 gccttaattc tgtaaaaaca gtaaaattcg aaggattgta aggtaagagt ttttttcttt
     1501 ctgaaaaaat ggtataatag caatcaaaac tagaaaataa aacggaattt ggaacagatt
     1561 tgtctgtatc ctagtagagt ggtgatacta tgaagattag taagaggcac ttattaaatt
     1621 attccatctt gattccctac ttgcttttat ctattttggg cttgattgtg gtctattcga
     1681 ccaccagtgc tattttaatt gaagaaggca agagcgcctt gcagttggtt cgaaaccaag
     1741 gaatcttttg gattgttagt ttgatactga ttgccttaat ttataaattg agactagatt
     1801 ttttgagaaa tgagcgacta atcattttag ttatattaat agaaatgctt ttattgttct
     1861 tggctcgttt tattggtatt tcagtaaacg gggcatacgg ttggatttcg gttgcaggag
     1921 taactattca gccagctgag tacttaaaaa tcattattat ttggtattta gctcaccgat
     1981 tctccaaaca gcaagaagaa atagctactt atgattttca agttttgact caaaatcaat
     2041 ggcttccccg tgcttttaat gattggcgat tcgttctcct agttctgatt ggaagtttgg
     2101 gaattttccc tgatttagga aatgcgacta ttttagtctt ggtttccttg attatgtata
     2161 cagttagtgg aatcgcttat cgctggtttt caaccattct ggcgctcgta tctgccactt
     2221 ctgtctttgt cttgaccact atcagcctaa tcggtgttga gaccttttca aaaattccag
     2281 tatttggcta tgtagccaag cgctttagtg ccttttttaa tccttttgcc gatcgtgctg
     2341 atgcaggtca ccagttagct aattcttatt ttgccatggt caatggtggt tggtttggtc
     2401 taggtcttgg aaactcgatt gaaaaacgag gttatttgcc agaagctcat acagactttg
     2461 tcttttctat cgtgattgaa gaatttggct ttgttggtgc cagtcttatt ttagctctct
     2521 tgtttttcat gattttgcgg attatcttgg tcggtatccg agcggagaat cctttcaatg
     2581 ccatggttgc actcggtgtc ggagggatga tgttggttca ggtatttgtc aatatcggag
     2641 ggatttcggg cttgattcca tctacaggag tgactttccc tttcttatcc cagggtggaa
     2701 atagtcttct agtcttatca gtggcagtag cctttgtctt aaatattgat gccagtgaaa
     2761 aacgcgctaa gttgtaccga gaattggaaa atcaaccaat gaaccttctg ttgaagtagg
     2821 ataaagaaag gatagtttat gtctcttcaa aaattagaaa attatagtaa taaaagtgtt
     2881 gtgcaagaag aagtcttgat tctaacagaa ttactggaag atattactaa aaatatgctt
     2941 gccccagaga cctttgaaaa aataatacag ttgaaagaat tatcaacgca ggaagattat
     3001 caaggtctaa accgtctagt gactagctta tcaaatgatg aaatggtcta tatttcacgc
     3061 tatttctcta tcttacctct tttgattaat atttcagagg atgtggattt agcttatgaa
     3121 atcaatcatc aaaataatat tgatcaggac tatttaggta aattatctac aacgattaaa
     3181 ttggtagcag aaaaggaaaa tgccgttgag atcctagaac acttgaatgt tgtccctgtt
     3241 ttgacagccc atccaacaca agtgcaacgc aaaagtatgt tggatttaac aaatcatatt
     3301 catagtcttt tgcgtaaata ccgtgatgtt aagttggggt tgatcaataa agataaatgg
     3361 tacaatgatt tgcgtcgtta catcgaaatt atcatgcaga cagacatgat tcgtgagaaa
     3421 aaattaaaag tgactaacga aatcacgaat gctatggaat attataacag ctcctttttg
     3481 aaagctgtac ctcatttgac gacggagtat aagcgcttag cgcaagcgca tggtctgaat
     3541 ttaaaacagg ctaaaccaat caccatgggt atgtggatag gtggtgaccg tgatggaaat
     3601 ccatttgtta cagcaaagac cttgaagcag tctgcactca ctcagtgtga agtcatcatg
     3661 aactactatg ataaaaagat ttaccaactt tatcgtgaat tttctctttc aactagcatt
     3721 gtcaacgtca gcaagcaagt cagagaaatg gctcgtcaat ccaaggataa ctcgatttac
     3781 cgcgaaaaag agctttaccg tcgtgccttg tttgatattc aatcaaaaat tcaggcaact
     3841 aaaacctatc tgattgagga tgaagaagtt gggactcgtt atgaaaccgc caatgatttc
     3901 tacaaggatt tgattgccat tcgagattct ctactagaaa ataagggcga gtccttgatt
     3961 tcaggtgatt ttgtggaatt attgcaggca gtagagatat ttggttttta cttagcatca
     4021 attgatatgc gacaagactc tagcgtctat gaagcctgtg tggcagaact cttgaaatca
     4081 gcaggaattc attctcgtta tagcgagttg agcgaagaag aaaagtgtga ccttctcttg
     4141 aaagaattag aagaagatcc ccgaattctt tctgcgactc acgcagaaaa atcagaatta
     4201 ttagcaaaag aattagctat ttttaagacg gctcgtgttt tgaaagataa gttgggagat
     4261 gatgtcatcc gtcagaccat catttcacat gcaaccagcc tttctgatat gctagaatta
     4321 gctattctgt taaaagaagt aggactggtg gatacggaaa gggcgcgtgt tcagattgtt
     4381 cccctttttg aaacaattga agacttggat cattcagagg aaacaatgag aaaatatctt
     4441 tctcttagcc ttgccaaaaa atggattgac tcacgaaata actaccaaga aatcatgctt
     4501 ggctactctg acagtaataa agatggcggt tacttgtcat catgttggac cctctacaag
     4561 gctcaacaac aattgactgc tattggagat gaatttggcg ttaaggttac cttcttccat
     4621 ggtcgtggtg gtactgtcgg tcgtggtggt gggccaacct atgaagccat tacatctcaa
     4681 ccgctcaagt ctatcaagga tcgtatccgt ttgacggagc agggtgaagt aattgggaat
     4741 aaatacggta acaaagacgc cgcttactat aaccttgaaa tgctagtatc tgcagctatt
     4801 aaccgtatga ttactcataa gaagagtgat accaataccc caaatcgtta tgaagccatt
     4861 atggatcaag tagtggaccg tagttacgat gtctaccgtg atttggtctt tggtaatgag
     4921 catttctatg attatttctt cgagtcaagt ccaatcaagg ctatttcaag ttttaatatt
     4981 ggttctcgtc cagccgctcg taagactatt actgaaatcg gtggtttgcg tgccatccct
     5041 tgggtattct catggtcaca gagtcgtgtt atgttccctg gatggtacgg ggttggttca
     5101 agcttcaagg aatttatcaa taaaaatcca gagaatattg ctatcttacg agatatgtac
     5161 caaaattggc ctttcttcca atcgcttctt tcaaatgttg atatggtttt gtcaaaatca
     5221 aatatgaata ttgcttttga atatgctaaa ctttgtgaag acgagcaagt taaggccatc
     5281 tatgagacta ttttaaatga atggcaagtt actaagaacg ttatcttggc tattgaagga
     5341 catgacgaac tcttagctga caatccatat ctaaaagcta gtctggatta ccgtatgcct
     5401 tactttaata ttctcaacta tattcagtta gagttgatta aacgccaacg tcgtggagaa
     5461 ttgtccagtg atcaagaacg attgattcat atcaccatca acggaattgc gacaggattg
     5521 cgtaattcag gttgataatt ttcaagagtg aatgctaaaa gtgaatatca aaaaaattct
     5581 aatagactat tgacaagtag tttaaaaatg atataattta accattcaga aaagtaatca
     5641 tacaaacttt ttagagagtc tgtggtagct gaaaacagat aagtggcaat gatgaaaatt
     5701 gggctgaatg ctatttagaa tttgaaatta taaaaattcg gtaagcacac cttacagtgc
     5761 atctcgttat tgcgagactg agcgataggg aaattcccta taattgaggt ggtaccgcgc
     5821 atcgacgtcc tcacacaagt tttttgtgtg aggatttttt tgatggaggt tagtatggaa
     5881 agaaaacaat ggcgtcgctt gtttagataa gtgaaatatg ttaaaggaaa taaaaaggag
     5941 aaacagaatg aaaaataaac gtttaattgg aattattgct gcattagcag tcttagtagc
     6001 aggaagcttg atttattctt caatgaataa atcagaagct cagaataata aggatgagaa
     6061 gaaaataacc aagattggtg tgcttcaatt tgtgagccat ccatcccttg atttgattta
     6121 taaagggatc caagatggac ttgcagaaga aggatataaa gatgatcaag ttaaaattga
     6181 ttttatgaac tcagaaggtg accaaagtaa ggttgcgaca atgagtaaac aattggttgc
     6241 aaatgggaat gaccttgtgg ttggtatcgc aacaccagca gcccaagggt tggctagtgc
     6301 aacaaaagac ctaccggtta tcatggccgc tattacagac ccaattggtg ctaacttggt
     6361 taaagatttg aaaaaaccag gtggcaacgt tacaggggta tctgaccaca atccagctca
     6421 acaacaagtt gaactcatca aggctctgac accgaatgtg aaaacaatcg gagctcttta
     6481 ctcaagtagc gaagacaatt caaaaacaca ggtcgaagaa tttaaggctt atgctgaaaa
     6541 agcaggtctg acagtggaaa catttgcagt tccttcaaca aatgaaattg cctcaactgt
     6601 cactgttatg actagcaagg tagatgctat ttgggttcca attgataaca ccattgcatc
     6661 aggatttcca acggttgtct ctagcaatca aagttctaag aaaccaattt atcccagtgc
     6721 gacagctatg gtagaagtag gtggtttggc atcagttgta attgaccaac atgaccttgg
     6781 tgtggcaaca ggtaaaatga ttgtgcaagt cttgaaaggt gcaaaaccag ccgatacccc
     6841 agtcaatgtc ttttcaactg gtaagtcagt catcaataaa aaaatagcac aagaactagg
     6901 tattactatt cctgagtctg ttctcaaaga agcaggacaa gtcatcgaat aaataaggta
     6961 gggaggagga gactcttccc attttttaaa aagatgaata tagaaaggat taaataaaat
     7021 atgattgttt ccattatttc tcaaggattt gtctgggcta ttctaggtct gggaatcttt
     7081 atgacattta ggattttaaa ctttccagat atgacgacag aaggttcctt ccctcttggg
     7141 ggagctgttg ctgtcacttt gataaccaaa ggcgtgaacc catttttagc gacacttgtt
     7201 gctgtaggag caggttgttt ggctggaatg gcagcaggcc ttctttatac aaaagggaag
     7261 atcccaacct tgctctcagg gattttggtg atgacttctt gtcactcaat catgctcttg
     7321 attatgggac gtgcgaattt aggcctgctt ggaaccaagc aaattcagga tgttttgcct
     7381 tttgattcgg atttgaatca actcttgaca ggtctcatct ttgtgagtat tgttattgct
     7441 ctcatgctct ttttcttgga cactaaactc ggacaagcct atattgctac aggggataat
     7501 cctgatatgg ctagaagttt cgggattcat actggacaca tggagctcat gggcttggtc
     7561 ttatcaaatg gtgtgattgc ccttgcaggt gccctcattg ctcagcaaga aggttatgcc
     7621 gatgtgtctc gagggatcgg ggttatcgtt gtggggcttg caagtttgat tattggagaa
     7681 gttattttca agagtttgag cttggcagag cgtttggtta ctatcgttgt aggttctatc
     7741 gcttatcaat ttttagtgtg ggcagttatc gcacttggct ttaatacaag ttaccttcgt
     7801 ttatacagtg ccttgatttt agcagtctgc ctcatgattc caacatttaa gcaaacaatc
     7861 ttgaaaggag ccaagttaag caaatgacag caattgtaga attaaaaaat gcaaccaaaa
     7921 tcgttaaaaa tggctttgat gaagaaaaga ttattttaaa tgatgtttcc ttagaaattt
     7981 ttgaacagga ctttatcacg attttgggtg gaaatggtgc tggtaaatca accctcttta
     8041 acactattgc aggaaccata tcactaacca gtggaactat ccgtatttta ggtgaggatg
     8101 ttaccaagtt ttcaccagag aagcgcgcta agtatctgtc tcgagtcttc caagatccaa
     8161 agatggggac agctcctcgt atgacagtgg cagagaatct cctgattgcc aagtttcgtg
     8221 gtgaaaagcg tggactattt ccgagacgtc tggctagcta taaggataag tttcaggcga
     8281 ccattgaaaa agtgggaaac ggtcttgaga aacacttgaa tacaccgatt gagttcttat
     8341 ctggtggaca aagacaggct ttgagtctct tgatggcaac cttgaagcga cctgaattac
     8401 tcttgttaga cgagcatact gctgccctgg atccaaagac tagtgttgct ttgatggaat
     8461 tgacagatga atttgttaag aaagatcaat tgacagccct tatgattacc catcatatgg
     8521 aagatgctct caaatatggc aatcgcttga ttgtcatgaa agaaggacgg gttatccaag
     8581 atttgaaaca agaagaaaaa gcaaaaatga aaatctctga ttattatcaa ctctttgaat
     8641 aaagtaggga aaatgagtga aatggtttac tttttttctg aaataaagta tactatataa
     8701 agtaaactat gataacatgg aggtattgtg tatggttgac aaacaagtca ttgaagaaat
     8761 caaaaacaat gccaacattg tggaagtcat aggagatgtg atttctttac aaaaggcagg
     8821 acggaactat ctagggctct gtccttttca tggtgaaaaa acaccttctt tcaacgttgt
     8881 agaggacaag cagttttacc actgttttgg ttgtggtcgc tcaggtgatg tctttaaatt
     8941 catcgaggag taccaagggg ttacctttat ggaggctgtc caaatcttag gtcagcgtgt
     9001 cgggattgag gttgaaaaac cgctttatag tgaacagaag ccagcctcgc cttaccaagc
     9061 tctttatgat atgcacgaag atgcggctaa attttaccat gctattctca tgacaacgac
     9121 tatgggcgaa gaggccagaa attaccttta tcagcggggt ttgacagatg aagtgcttaa
     9181 acatttttgg attggtttag cacctccaga acgaaactat ctctatcaac gtttgtctga
     9241 tcagtatcgt gaagaggatt tactggattc aggcctgttt tatctctcgg atgccaatca
     9301 atttgtagac acctttcaca atcgcattat gtttcccctg acaaatgacc aaggaaaggt
     9361 cattgccttc tcaggtcgta tctggcaaaa aacggattca caaacttcta agtataaaaa
     9421 cagccgatcg actgcaattt ttaacaaaag ttacgaatta tatcatatgg atagggcaaa
     9481 aaaatcttct ggaaaaacta gtgagattta cctgatggaa ggattcatgg atgttattgc
     9541 agcctatcgg gctggaatcg aaaatgctgt ggcgtcgatg ggaacggcct tgagtcgaga
     9601 gcatgttgag catctgaaaa ggttaaccaa gaaattggtt cttgtttacg atggagataa
     9661 ggctgggcaa gccgcgatat tgaaagcatt ggatgaaatt ggtgatatgc ctgtgcaaat
     9721 cgttagcatg cctgataact tggatcctga tgagtatcta caaaaaaatg gtccagaaga
     9781 cttggcctat ctattaacaa aaactcgtat tagtccgatt gagttctaca ttcatcagta
     9841 caaacctgaa aacagtgaaa atctgcaggc tcagattgag tttattgaaa aaatagctcc
     9901 cttgattgtt caagaaaagt ccatcgctgc tcaaaacagc tatattcata ttttagctga
     9961 cagtctggcg tcctttgatt atacccagat tgagcagatt gttaatgaga gtcgtcaggt
    10021 gcaaaggcag aatcgcatgg aaagaatttc cagaccgacg ccaatcacca tgcctgtcac
    10081 caagcagtta tcggctatta tgagggcaga agcccatcta ctctatcgga tgatggaatc
    10141 ccctcttgtt ttgaacgatt accgtttgcg agaagacttt gcatttgcta cacctgaatt
    10201 tcaggtctta tatgacttgc ttggccagta tggaaatctt cctccagaag ttttagcaga
    10261 gcagacagag gaagttgaaa gagcttggta ccaagtttta gctcaggatt tgcctgctga
    10321 gatatcgccg caggaactta gtgaagtaga gatgactcga aacaaggctc tcttgaatca
    10381 ggacaatatg agaatcaaaa agaaggtgca ggaagctagc catgtaggag atacagatac
    10441 agccctagaa gaattggaac gtttaatttc ccaaaagaga agaatggagt aataatggca
    10501 acaaaacaaa aagaagtaac aacatttgac gtacaggtag cagaatttat ccgtaatcat
    10561 aagcaaaaag ggacagcaac agatgatgaa atcaatgcaa gtctggttat tccttttacc
    10621 ttggacgctg atgggattga agatctcttg caacggattc aggatgcagg catttctatc
    10681 acagataacg aaggaaatcc aagtgcgcgt gttcttagca atgaagaaga accagaactc
    10741 agcgatgagg acttgattgg gtcaacttct gctaaggtca atgaccctgt ccgtatgtac
    10801 ttgaaagaaa taggggtcgt tcctctcttg accaatgaag aggagaaaga gttggcactg
    10861 gctgttgaag ctggcgatat cgaagccaaa caacgtcttg cggaagccaa tcttcgtttg
    10921 gttgtttcca ttgccaaacg ctatgtcggt cgtggcatgc agttccttga cttgattcaa
    10981 gaaggaaata tgggcttgat gaaggcggtt gacaagtttg actattctaa agggttcaag
    11041 ttttcaactt atgcaacttg gtggattcgt caggctatca ctcgtgctat tgcggaccaa
    11101 gctcgtacca tccgtatccc agttcacatg gttgaaacta tcaataaatt ggttcgtgaa
    11161 cagcggaatc tccttcaaga attggggcaa gatccgacac cagaacagat tgctgaacga
    11221 atggatatga cacctgataa ggttcgtgaa atcttgaaga ttgcccaaga accagtatct
    11281 cttgaaactc ctatcggtga agaggacgat agccaccttg gagactttat cgaagatgaa
    11341 gtgattgaaa atccagtgga ttatacgact cgtatcgtct tgcgtgagca attggatgaa
    11401 atcttagata ctcttacaga ccgtgaagaa aatgttctgc gtctacgttt tggactagat
    11461 gatggaaaaa tgcgcacact tgaagatgtg gggaaagtct ttaacgtaac tcgtgagcgt
    11521 atccgtcaga ttgaagcaaa ggctttgaga aaactacgcc aaccaagtcg cagcaaaccg
    11581 cttcgtgatt ttattgaaga ctaagagtga ggaaaatatg gcttatacag aagagcaaat
    11641 tgaaaacatc aaaacacgga ttttaacagc cttggaagaa gtcatcgacc ctgagttggg
    11701 aatcgatatt gtcaatcttg gtttgatcta tgagattcgt tttgacggtg acacagggca
    11761 aacagagatt gacatgactt tgacaactat gggttgtccc ttagcagacc ttttgacaga
    11821 ccagatttat gatgctatga tagaggttcc agaagtaacg gatactgagg ttaaattagt
    11881 ctggtatcca gcctggacag ttgaaaaaat gagtcgctat gcccgcattg ccctaggcat
    11941 taagtaaaca tataaaagca tgtgagagac tgttggaaaa gcgaggaaat ttcccctctt
    12001 ttcctctagt ctctcctttc ttttgctgat tttattcaaa gaaaatgata taatagtagt
    12061 tatggagaaa aagaaattac gcatcaatat gttgagttca agtgagaaag tagcaggaca
    12121 gggagtttca ggtgcttacc gtgaattagt tcgtcttctt caccgtgctg ccaaggacca
    12181 attgattgtt acagaaaatc ttccgatcga ggcagatgtg actcactttc atacgattga
    12241 ttttccctat tatttatcaa ccttccaaaa gaaacgctca gggagaaaga ttggctatgt
    12301 gcatttcttg ccagctacac ttgagggaag tttgaaaatt ccatttttct taaagggaat
    12361 tgtgaaacgc tatgtatttt ctttttacaa ccggatggag cacttggttg tggtcaatcc
    12421 tatgtttatt gaggatttgg tagcagctgg tattccacgt gaaaaagtga cctatattcc
    12481 taactttgtc aacaaggaaa aatggcatcc tctaccacaa gaagaggtag tcagactgcg
    12541 cacagatctt ggtcttagtg acaatcagtt tatcgtagta ggtgctgggc aagttcagaa
    12601 acgtaaaggg attgatgact ttatccgtct ggctgaggaa ttgcctcaga ttacctttat
    12661 ctgggctggt ggcttctctt ttggtggtat gacagatggt tatgaacact ataagaaaat
    12721 tatggaaaat ccccctaaaa atttgatttt tccaggcatt gtatcgccag agcggatgcg
    12781 cgaattgtat gctctagcgg atcttttctt gttgcctagt tacaatgagc tctttcctat
    12841 gactatttta gaagctgcga gttgtgaggc tcctattatg ttgcgtgatt tagatctcta
    12901 taaggtgatt ttggagggaa attatcgggc gacagcgggt agagaagaga tgaaagaggc
    12961 tattttggaa tatcaagcaa atcctgctgc cttaaaagat ctcaaagaaa aggctaagaa
    13021 tatttccaga gagtattctg aagagcatct gttacaaatc tggttggact tttatgagaa
    13081 acaagccgct ttagggagaa agtaaaaagt gaggtaatct atgcgaattg gtttatttac
    13141 agatacctat tttcctcagg tttctggtgt tgcgaccagt attcgaacct tgaaaacaga
    13201 acttgaaaag cagggacatg ctgtttttat ctttacgacg acagataagg atgtcaatcg
    13261 ctacgaagat tggcaaatta tccgcattcc aagtgttcct ttctttgctt ttaaggatcg
    13321 tcgctttgcc taccgaggtt ttagcaaggc acttgaaatt gctaaacagt atcagctaga
    13381 tattatccat actcagacag aattttctct tggcctgttg gggatttgga ttgcgcgtga
    13441 attgaaaatt ccagtcatcc atacctatca cacccagtat gaagactatg tccattatat
    13501 tgctaagggg atgttgatcc gaccgagtat ggtcaagtat ctggttagag gtttcctgca
    13561 tgatgtggat ggggttattt gccctagtga gattgtccgt gacttgctat ctgattataa
    13621 ggtcaaggtt gaaaaacggg tcattcctac tgggattgaa ttagccaagt ttgagcgtcc
    13681 ggaaatcaag caggaaaatt tgaaagaact gcgtagtaaa ctagggattc aagatggtga
    13741 aaagacgttg cttagtcttt cgagaatctc ctatgaaaaa aatattcaag cagttttagt
    13801 agcctttgct gatgttctga aagaggaaga caaggttaaa ctggtagtag ctggggatgg
    13861 cccttatctg aatgacctca aagagcaagc ccagaaccta gagattcaag actcagtcat
    13921 ctttacaggg atgattgctc ctagtgagac ggctctttac tataaagcgg cggatttttt
    13981 catttcggca tcgacaagcg aaacgcaagg tttgacctac ttggaaagct tagccagtgg
    14041 aacacctgtc attgctcacg gaaatcctta tttgaacaac ctcatcagtg ataaaatgtt
    14101 tggaaccttg tactatggag aacatgattt ggctggtgct attttggaag ccctgattgc
    14161 aacaccagac atgaacgagc ataccttatc agagaaattg tatgagattt cagctgagaa
    14221 ctttgggaaa cgagtgcatg agttttatct ggatgccatt atttcaaata acttccagaa
    14281 agatttggct aaagatgata cggtcagtca gcgtatcttt aagacagttt tgtatcttca
    14341 gcaacaggtg gttgctgtac ctgtaaaagg atctagacgc atgttgaagg cttcaaaaac
    14401 acagttgatc agtatgagag actattggaa agaccatgaa gaatagaaag aggaacagct
    14461 atgaaaaaaa caattaatga gaagcggtcg tgataaaaag attgcgggtg tttgtgctgg
    14521 ggtggcccat tatctggata tggatccgac tatcgttcaa gtcatttggg gtgttcttac
    14581 ttgctgttac ggagctggaa ttgtagctta cattatttta tggattatcg caccagtagc
    14641 aactgactat tgaaaactag ctcaattcat gctaagataa tagaaaatag aatgtaacga
    14701 aggagagaaa aatggcattt ggagataatg gaaatcgtaa aaaaactatg tttgagaaaa
    14761 taaccttgtt tatcgtgatt atcatgctag tagcaagttt attgggaatt tttgcaactg
    14821 caattggtgc cctcagtaat ctataaaata gattcaagaa aatttagtga ctgggatttc
    14881 ccagcccttt tttaaagtga gaagaaataa tgagtatgtt tttagataca gctaagatta
    14941 aggtcaaggc tggtaatggt ggcgatggta tggttgcctt tcgtcgtgaa aaatatgtcc
    15001 ctaatggagg cccttggggt ggtgatggtg gtcgtggagg caatgtggtc ttcgttgtag
    15061 acgaaggact acgtaccttg atggatttcc gctacaatcg tcatttcaag gctgattctg
    15121 gtgaaaaagg gatgaccaaa gggatgcatg gtcgtggtgc tgaggacctt agagttcgag
    15181 taccacaagg tacgactgtt cgtgatgcgg agactggcaa ggttttaaca gatttgattg
    15241 aacatgggca agaatttatc gttgcccacg gtggtcgtgg tggacgtgga aatattcgtt
    15301 tcgcgacacc aaaaaatcct gcaccggaaa tctctgaaaa tggagaacca ggtcaggaac
    15361 gtgagttaca attggaacta aaaatcttgg cagatgtcgg tttagtagga ttcccatctg
    15421 tagggaagtc aacactttta agtgttatta cctcagctaa gcctaaaatt ggtgcctacc
    15481 actttaccac tattgtacca aatttaggta tggttcgcac ccaatcaggt gaatcctttg
    15541 cagtagccga cttgccaggt ttgattgaag gggctagtca aggtgttggt ttgggaactc
    15601 agtttctccg tcacatcgag cgtacacgtg ttatccttca catcattgat atgtcagcta
    15661 gcgaaggccg tgatccatat gaggattacc tagctatcaa taaagagctg gagtcttaca
    15721 atcttcgcct catggagcgt ccacagatta ttgtagctaa taagatggat atgcctgaga
    15781 gtcaggaaaa tcttgaagaa tttaagaaaa aattgtctga aaattatgat gaatttgaag
    15841 agttaccagc tatcttccca atttctggat tgaccaagca aggtctggca acacttttag
    15901 atgctacagc tgaattgtta gacaagacac cagaattttt gctctacgac gagtccgata
    15961 tggaagaaga agcttactat ggatttgacg aagaagaaaa agcctttgaa attagtcgtg
    16021 atgacgatgc gacatgggta ctttctggtg aaaaactcat gaaactcttt aatatgacca
    16081 actttgatcg tgatgaatct gtcatgaaat ttgcccgtca gcttcgtggt atgggggttg
    16141 atgaagccct tcgtgcgcgt ggagctaaag atggggattt ggtccgcatt ggtaaatttg
    16201 agtttgaatt tgtagactag gagactggta tgggagataa accgatatct ttccgagatg
    16261 cggatggtaa ttttgtttcc gccgcagacg tttggaatga aaagaaattg gaagaactat
    16321 ttaatcgtct caatccaaat cgtgccttga gattggcacg aactaaaaag gaaaatccat
    16381 ctcagtaaag aagctaaaaa atcccggctc tttgtcaact gtagtgggtt gaaaaaaagc
    16441 taagcttgag aaaggacaaa ttttgtcctt tcttttttga tgttcagagc gatgaaaatc
    16501 cgttttttga agttttcaaa gtttcgaaaa ccaaaggcat tgcgcttgat aagtttgatg
    16561 agattattgg tcgcttccag tttggcgtta gaatagtgta gttgaagggt gttgacaagc
    16621 ttttctttat ctttgaggaa ggttttaaag acagtctgaa aaataggatg aacctgctta
    16681 agattgtcct caataagtcc gaaaaatttc tccggttcct tattctgaaa gtgaaacagc
    16741 aagagttgat agagctgata gtggtgtttc aagtcttgtg aataactcaa aagcttgttt
    16801 aaaatctctt tattggttaa gtgcatacga aaagtaggac gataaaatcg cttatcactc
    16861 agtttacggc tatcctgttg aatgagcttc cagtagcgct tgatagcctt gtattcatga
    16921 gactttcgat gaaactgatt catgatttga acacgcacac gactcatagc acggctaaga
    16981 tgttgtacaa tgtgaaagcg atcaagaaca atttttgcat tcgggagtga aacagtctgg
    17041 gagactgttt cagcctgagc ctagaaattt gaaagcgaag ttgtttagcc aagtcatagt
    17101 aaggactaaa catatccatc gtaatgattt tcacttgaca acgaacggct ctatcgtagc
    17161 gaagaaagtg atttcggatg acagcttgtg ttctgccttc aagaacagtg ataatattaa
    17221 gattatcaaa atcttgcgca atgaagctca tctccatctc ccgattgaaa cagtcactcc
    17281 ccggactgtt tcaacctccc aagacataat ctcaggcaga tttctaaaat tacactcaaa
    17341 gtggaagtca ttgagcttgc gaatgacagt tgaagttgaa atggccagct gatgggcaat
    17401 atcagtcata gaaatttttt caattaactt ttgagcaatt ttttggttga tgatacgagg
    17461 gatttggtga tttttcttta
//
LOCUS       DAVHIT010000039        17013 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVHIT010000039 DAVHIT010000000
VERSION     DAVHIT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.040"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..186
                     /locus_tag="VVN16_001156"
     CDS             <1..186
                     /locus_tag="VVN16_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5572552.1"
                     /translation="EDAKNKKSYDRLAICYVRIGICRDNAKLIQKGFSLLELTEETSM
                     LSHLKKEVETHYQPKKL"
     gene            complement(476..946)
                     /locus_tag="VVN16_001157"
     CDS             complement(476..946)
                     /locus_tag="VVN16_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5572553.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(912..1709)
                     /locus_tag="VVN16_001158"
     CDS             complement(912..1709)
                     /locus_tag="VVN16_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5572554.1"
                     /translation="MNKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALNPD
                     FAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLAI
                     SGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNFEKE
                     QDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(1916..2557)
                     /locus_tag="VVN16_001159"
     CDS             complement(1916..2557)
                     /locus_tag="VVN16_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572555.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(2554..4662)
                     /locus_tag="VVN16_001160"
     CDS             complement(2554..4662)
                     /locus_tag="VVN16_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5572556.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(4717..5010)
                     /locus_tag="VVN16_001161"
     CDS             complement(4717..5010)
                     /locus_tag="VVN16_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU5572557.1"
                     /translation="MIGEINHEKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            5417..5737
                     /locus_tag="VVN16_001162"
     CDS             5417..5737
                     /locus_tag="VVN16_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5572558.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLVICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(5789..6376)
                     /locus_tag="VVN16_001163"
     CDS             complement(5789..6376)
                     /locus_tag="VVN16_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5572559.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            6618..7289
                     /gene="sdaAB"
                     /locus_tag="VVN16_001164"
     CDS             6618..7289
                     /gene="sdaAB"
                     /locus_tag="VVN16_001164"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU5572560.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            7298..8170
                     /gene="sdaAA"
                     /locus_tag="VVN16_001165"
     CDS             7298..8170
                     /gene="sdaAA"
                     /locus_tag="VVN16_001165"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU5572561.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            8192..8824
                     /locus_tag="VVN16_001166"
     CDS             8192..8824
                     /locus_tag="VVN16_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5572562.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(9082..10932)
                     /locus_tag="VVN16_001167"
     CDS             complement(9082..10932)
                     /locus_tag="VVN16_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU5572563.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(10974..12131)
                     /locus_tag="VVN16_001168"
     CDS             complement(10974..12131)
                     /locus_tag="VVN16_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5572564.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLSGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            12189..13355
                     /locus_tag="VVN16_001169"
     CDS             12189..13355
                     /locus_tag="VVN16_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU5572565.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            13494..13820
                     /locus_tag="VVN16_001170"
     CDS             13494..13820
                     /locus_tag="VVN16_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5572566.1"
                     /translation="MYFPTSSALIEFLILAVLEKGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSRDFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            13807..14400
                     /locus_tag="VVN16_001171"
     CDS             13807..14400
                     /locus_tag="VVN16_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU5572567.1"
                     /translation="MTRTEYLTQLELYLKKLPEADQIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHVALLALLAAPIGIPLGIAILV
                     SLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGDQHA"
     gene            14393..15304
                     /locus_tag="VVN16_001172"
     CDS             14393..15304
                     /locus_tag="VVN16_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEU5572568.1"
                     /translation="MRKLTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLAMSKEPVY
                     DSRMEELTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSESPFISSGIDGILHIVSSSSNRFNDVIIGLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNGYILRIEGSRIKNSKLTTPNIVNIFDTDLTDSQLESTEHHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDVSSNYGSIFQFTREEPESRGTELSNPYK
                     TEKTDVKDQLIARSDDDIELISTPSRR"
     gene            15366..16430
                     /locus_tag="VVN16_001173"
     CDS             15366..16430
                     /locus_tag="VVN16_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572569.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEERIADLDTPIEK
                     NTQVEEEVSQAEVELESQQEEKMETPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEALK
                     SPTSKLETSSTHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIATINSHFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIYQEKDWTLEKILQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFSALLCVISIGIILLASLHIITRPSQASEADSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGNYLRILAFL"
     gene            complement(16667..16864)
                     /locus_tag="VVN16_001174"
     CDS             complement(16667..16864)
                     /locus_tag="VVN16_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HEU5572570.1"
                     /translation="MRSLSQEKDIYSFDEPTGNLDRNSTELFLNEVEKLVNEEKIVIV
                     VTHDKDVIARASKVINMDEFH"
BASE COUNT         5159 a         3759 c         2984 g         5111 t
ORIGIN      
        1 gaggacgcca agaataagaa aagctacgat aggcttgcta tctgctatgt tcgtattggg
       61 atttgtaggg ataatgctaa acttatccaa aaaggtttct cactcctaga gctgaccgag
      121 gaaacttcta tgctgtctca tctcaaaaaa gaagtagaga cccattatca accaaagaaa
      181 ttataaaaaa agtcgaggga tttcctcgac tctttacata ttatttgttc gttagttctt
      241 aataccagcc attgttaaac cagaagtttt tagctctgcc aagccacata gtgtgtttac
      301 tactatgtgt ccatgtcacc atgaatcgcc gaacgattta acgagaaagt atgactttta
      361 cgtttatccc aactcaatta tgacattttt ttcaaaagtc aatatatctc actttttcaa
      421 cgacaagaaa gaggctgcca attgaccaac ctcttattct gaacccatca ctccatcact
      481 ttttagcttc attcgctttc ttagcgactg caatctggta ttcgacttgg tcattcccct
      541 tacctgtaca accatgagca attgtagtcg ctcctgtctg atgcgctatt tcaaccaatt
      601 ttttagaaat aagagggcgg ctcaaggcag ataccaaggg gtacttttgt tcatagtagg
      661 catgtgactg gagagctact agcacataat ctgtagcaaa ttcgtcctta acatcaatga
      721 cataagattc gactgcccca accttaagag ccttatcatg gatgaaatct aagtctttcc
      781 cttcacccac atccatacaa actgcaacaa catcatagtc tttcttcagc caggtaatag
      841 caactgatgt gtctaaaccg ccagaatagg ccaaaattac tttttcttta ctcatgttct
      901 tttccttctt tctatttttc aatggacgct ttaaaggcat cctcaatgag tttgtctaat
      961 tccccagact ccttcaactt ttgaatggtt ttatcgactg cctctttcaa ttccttgcta
     1021 tcttttttca tggctaccgc ataggaatca tcttgctctt tttcaaaatt gaggtctgcg
     1081 attgctaaat caggattatt ttccacaaat cccttggcaa cgggttcttc aaaaataacg
     1141 gcatccactt gccctgattt taaatctgtg attaaattcc catttttagg cagagatacg
     1201 agggaagaat tttgtagcaa atctttcgcc atcgtctctt gaatcgaacc tttctgcgct
     1261 ccgacctttt tctgcgccaa atcgttgaca gactgataag tggctaaatc agattttttg
     1321 acaatgagtt tattttttgc agtatagtag ggagttgaaa aatcaaacac cttgctccgt
     1381 tcatctgtct tagaaacacc tgatatggca aggtcagctt ttcctgattg aacgctagcc
     1441 agtacattgt caaaactcat aggagatagt tccaattcga cacctagttc tgttgcgata
     1501 gccttggcta attcgatatc tgaacccaca atctgatttt tcccatcaac cactttttga
     1561 tattcaaatg gagcaaaatc tggattgagg gctacaacca attttccttt ggacttgatg
     1621 gcttcaattc cagccgattg attgttacta caagcaaata gtagggggag catcactaaa
     1681 ccaaacatcg tcatcaacac cttattcata ataaagcctc ctttatgttt attctttttt
     1741 attgtataaa taatactcct atactcatcc tttgtcaagc ctaaattgaa aaaattttca
     1801 atttgaaaca aaaaaaacct agaaatacta cataaaatgt cattcctagg cgtatttatg
     1861 ctatttttct ctggaaaaca tgagtattca gccagtcaaa aggagctgaa tgaactcatt
     1921 ttccctcgcc caattcaatg acacgatgac attgttgggc tacataagca tcgtgggtca
     1981 caataatgac tgttttccct tctcgattca tctctaagag aaacttcaag accaaatctc
     2041 tattttcagg atccagcgaa cctgtaggtt catcggctaa aatcagctgg ctgggtttta
     2101 agatggctct agcaactgca attcgttgtt gttcgccccc agacaactcg gagacccttt
     2161 gatgcaaagt agctgacaaa cctactctct ctaaaatctc ttccaccttt ttgagcttgt
     2221 ctttcttagg caatttcaca tatttcagcg ccagcatgag attgtactcg accgtttcat
     2281 catcaatcag ggcaaaattt tgaaacagat aagagatatg ttcacggatt attgtttgcg
     2341 acttagcaga attaaccgct agatttgtct gaccaaaaat ctcataccgt ccgctataat
     2401 caccatctat caaacccaat aaatttaaca aggtcgactt cccactacca ctcttaccaa
     2461 caatagctac caaatccccc tgatcaatcc tgagagataa gttatccaaa atcacttttc
     2521 ccccaatggt tttggtaata tttttcaact caatcataag atgccccctt tcaataactc
     2581 tactagactt cttttctcca tcctagaagc taagcctagc acaaatagta tatccagaca
     2641 tgtaaaacct gcaaacagta gaagtggtaa gaacgcatgg gcaaagaaaa tcaagactag
     2701 aagagggaaa ctatagccca gcaagagcag aacgaggaga ggacggtagc gatcgaccag
     2761 tttccacccc ataaacttct tggtaatgat atccctgcgc ttcaataaga aagttgttac
     2821 tagtaagaag taggaaatca tcatgctaag gagaccaaac aaagcaaaga gtaggttaaa
     2881 attccgaaca gcatctcgat aagaatccac tttctcttgt tgaatggctt gaatagatga
     2941 aaattttaaa taatttccat ctgacaattt ctcaactaac tctgtaatct ctttttgatg
     3001 ttgaaccgta ttttcaattt taatcggatt atttaagcca gttgttgaca gggaggcttt
     3061 ctcatcccac atcatatcag aatcattgac caagctaata attggattgg agagattttc
     3121 ctttcgctta tcactatatg ggaaaaatga ccaatctcct tcataatagg caatctcgac
     3181 atccatctcc tctatcgttc gtttttgctg ctcttcatac ttcatcgaat gaaaggcaat
     3241 taacttcccc aagagctgat ttttatcttc ttcacctttc gtacttgctg gcatcaaaat
     3301 aactttttta ataccggtat ctggtagctt gaatcccttg ctctttagaa aattgcgatt
     3361 ggcatagtaa acatccaccg tatctgttaa ctgatattgc tgaatctgtt ctgattggac
     3421 aaaatttttt acaggaagac tgctactctg cacatagccc gcctgcgttt tttctaccaa
     3481 atcctgataa aatcgataga aataatccaa tgccttccct gagcctgcca actgctcttg
     3541 ccacaagtta tcattgagtt ggaaagtttc taaggtcaga taatttcctt ggctgatcca
     3601 ctgtttctgg taatccaact ctttgttttc ttgctccaaa ctttttccta ccccaactag
     3661 caagactgtc agtaaaatgg tagttccaat tttcatcaaa taattaaaga gtagaccaaa
     3721 tttgaaagat gaaaaacctt tcagcagaga gctgattgtc attttttgga ttaagaggta
     3781 agtcaaccaa ctgataaaga gataaagctg caacagcaaa aaatgagaca accacagcat
     3841 aggaaacaaa tcttttggct tataatcaag caagaaaaac acgcctagat tgatcacaag
     3901 agccccacct aggaggaggt aaaggttgcc ttttacaaca tcagctaaaa cagccctatc
     3961 ttgaaaacca agtaattttt gtaccccaac tcgtttcatc tccatcatcg gttgatacac
     4021 tgtcactaac acaagaagca aaatagccaa gacaaaaaca atggcagata aaagcaaatc
     4081 tcgatttatg acttccactg cacttttgta ggtcggctct agcaaggtag cctggtctat
     4141 cttgaaaaaa tcgctccatt tctgtacaat cctatccttg tccatctctt gtgtagaagt
     4201 tatcgtatag cgaccattta aactacgaga tgtatccttg atataggttt gaaaagtcat
     4261 aagctgaata ggtttggctt ttagaaaggt cggaatcgta ccaagtttat tggaaatttc
     4321 tttattacta tagactcctt caccatctgt ggtaaaatca agagaagaaa tcccaaactc
     4381 ttggtagggg aaggtatctt tatcaaaaac accagacttg accacctcat caccactgtc
     4441 tgttttgatg atggagactt tatactcctt tgatacatcc tcaaaaaatc gaagaacaga
     4501 cgctgcaggt tcgttaatat ctttcaaata caaatccaaa gaatctacag tctttcccat
     4561 ttcttgaatc cgaaccactt ctcgtgtata gtttacatta aaaacaaaaa aagtggtaca
     4621 cagaagcagg agtaacatac aaagatgact gatttttttc atagagatac ctcccgtgaa
     4681 gaaactccct ctccaaaagg ggagggagaa ttttaattaa aatccataga aatattccat
     4741 tcccgttggt gggaatttga agattgaagc ccgagcccat gcctcagctt ctgcacgatc
     4801 cttagagaat ttatttccat ctttaacagt tgctgtatga gagcgagtag aatgtaaata
     4861 atcagaataa ccaaaatttc ctgaccatcc tactccatag ttccattgac caccatcaac
     4921 ccatactgcc aaaacacttg tagaagcaag tatactacat accattaaag atgcaacacc
     4981 aattttctca tgatttattt ctcctattat cgagagctat tccgtagaaa cagctatagt
     5041 aatttgtcaa caagtatcgt agtccattgg actcaccttc cttctatctc atttcatttt
     5101 taaacaacga acttactctt aactagaaga atagttctct tctgtatcag gtctttgaac
     5161 aaccagagta gtattaatca cactctaact attactcaag aatcagccca ataaacaata
     5221 tggatagcgc tttcaaattt ttaaactctt atcccatcct tttatctata taataagtga
     5281 aaatataata actgtcaagt aatcgaagtg aattttataa aaaaaattac aagccaaatt
     5341 tgcaaagttt acaataagcc gctagggcaa tcgtctatca gaatgtccgt ttatttgtca
     5401 ataatccgag aaaatcttgc aacgcttaga agtctataaa aactatcaac atttatatga
     5461 cttgcgaata gcaatcctgc tcaacctttc cacactctat ctatacaatc aagataaaaa
     5521 catgtgtaag caaatctgct acactttact agaggacgcc aagaataaga aaagctacga
     5581 taggctggtt atctgctatg tccgtattgg gatttgtaca gacgattcta aacttatcca
     5641 aaaagggttc tcccttctgg agctaaccga ggaaacttct atgctgtctc atctcaaaaa
     5701 agaagtagag atctattacc aagcgaagga aagataaaaa agccgaggga tttcctcgac
     5761 tctttacata ttatttgttc gttagttctt aataccagcc attgttaagc cagaagtttt
     5821 tagcagcagt ccatgaaccg taacgtcctg caacgtaggc atctgctaca cgttcttggt
     5881 tttcagctga gtagtcaccg ttcaggtatg aatctgttaa ttggtaacgt ccgatataac
     5941 gtccatttgt agctgtatag ctaccacctg attctttttg agcgatccat tctttggctt
     6001 ctgcttcaga tccgcttaca gttgaaacaa ctgtttcact tactactgga gtttctgcta
     6061 ctggagctga aacagtttca tcttgagcgg ctggtgccgc ataagtagct ggcgctggtg
     6121 ttgcaacagg cgctacaggg ccatcgataa ccaactcttg accaacataa atcaaatgaa
     6181 tgttatcaat gtggttgttt tctgccaatt tttcaactgt tgtgttgtga gtttcagcga
     6241 tttctgaaag tgtatcacct tctttaacag tgtaagttga tgattcttga gcagatacaa
     6301 atgatggagc aaatactgca aacaaggcag ctactcctgc aagagttgct ttaatctttt
     6361 tagttattga tttcatattc gaaaattctc cttctttcta tagttctatc atacccttta
     6421 aatattaccg tttcttgaca cttttatgta gaaatattac aaaattattt tttatttccc
     6481 taaataaggt gttttttgtc ataaaagata ctatttttat gctatttgag gtattagaaa
     6541 ggttttcatc cacaattaaa gccaagcccc tcattctttg atataataga gataagaatt
     6601 tttataaggg gaaactgatg aaatcacttc gttttcaatc tgtctttgat atcatcggac
     6661 cagttatgat tggcccatct agtagccata ccgctggtgc tgttcgtatt gggaagattg
     6721 tctcttccat ttttgatgat actccgacag aagttgaatt ccaacttttt aactcatttg
     6781 ccaagaccta tcgtggtcac ggaacagacc tagcccttgt tgcaggtatt ttaggaatgg
     6841 atacagatga tcctgaaatt ccaaatagtc tggaaattgc ccacaagcgt ggtatcaaga
     6901 ttgtctggac cattcagaaa gacagcaatg cccctcaccc taacaccact aaaattactg
     6961 ttaaaaatgc ccacaagacc atcagcgtga ctggtatttc tatcggtgga ggaaatattc
     7021 aggtaaccga actcaatggt tttgccgtct ctctcaatat gaatacaccg actatcatca
     7081 tcgttcatca agatattcca ggtatgattg cccttgttac agaggcgctt tcccgctatg
     7141 gtatcaatat cgcccagatg aatgtcactc gtgaaaaagc tggtgaaaaa gccattatga
     7201 ttatcgaagt tgacagtcgc aactgtgatg aggctatcga agaaattcga aaaatccctc
     7261 atctccacaa tgtcaatttc tttaaatagg aggaagcatg ttttattcta tcaaagaatt
     7321 ggtcgagcaa gcagatctag actttcaagg aaatgtcgca gaactcatga ttacaacaga
     7381 gtttgaattg accggtcgcg aacgtgaaga agtcttcctt ctcatggaac gcaatctgga
     7441 agttatgaaa gcctctgtcc aacttggcct caatgaaaat aaatctcgta gtggcctgac
     7501 aggtggagat gctgccaaat tggatcacta catcgaaaac ggaaaaactc tgtcagatta
     7561 tacgattctc tcggctgccc gaaatgccat cgcagtcaat gaacacaatg ccaaaatggg
     7621 cttggtctgt gccactccaa ccgctggaag tgctggctgt ctgccatccg ttctcactgc
     7681 tgctattgaa aaattagacc tcagccacga gcaacaattg gatttcctct ttgctgctgg
     7741 tgcctttgga ctagtcatcg caaacaatgc ctccatctca ggtgctgagg gtgggtgtca
     7801 agctgaagtt ggttcagcct ctgctatgag tgctgccgcc ttgactctgg ctgcaggtgg
     7861 aacaccttat caggccagtc aagctattgc ctttgtcatt aaaaatatgc taggcctcat
     7921 ctgtgaccct gttgcaggtt tggtcgaagt tccctgtgtc aaacgtaatg ccatgggagc
     7981 tagctttgct ttcatcgcag cagacatggc cttggcaggt atcgaatcta aaatccctgt
     8041 ggatgaagtg atcgatgcca tgtaccaagt aggagcaagc atgccaactg cctttcgtga
     8101 aacagctgaa ggtggactcg ctaccacccc tactggtcgt cgcctccaaa aagaaatttt
     8161 cggagaataa gcttatcaaa taggagaaac catgacctct atcacagcga tttttttcga
     8221 tttggatgga accctcgttg atagttctat cgggattcac aatgccttta cctatacctt
     8281 taaagagctg ggggtgccta gccctgatgc caaaactatt cgtggtttta tgggaccacc
     8341 tctcgaaagt agttttgcga cctgcctgtc caaagaccaa atttctgaag ccgtgcagat
     8401 atatcgttct tactataagg caaaaggcat ctatgaagct caactctttc ctcagattat
     8461 agacttgctt gaggagttat cgagcagtta tccactctac atcaccacga caaaggatac
     8521 atctactgct caagacatgg caaaaaactt ggaaatccat catttctttg atggcattta
     8581 tggttctagc cctgaagcac cccataaggc agatgtcatt caccaagcct tgcagacaca
     8641 tcaactagca ccagaacaag ccatcatcat cggagatacc aagtttgata tgctgggagc
     8701 tcgagaaaca ggtattcaga aattggccat cacttgggga tttggagagc aagcagatct
     8761 actaaactat caacctgatt atatcgctca caaaccctta gaagttttgg cgtattttca
     8821 atagcataga ctgggtaaca attccatttc agaagaaaat gaagtaatac tcaatgaaaa
     8881 tcaaagagca aactaggaaa ctagctgcgg gctgctctaa acactgtttt gaggttgtgg
     8941 atagaactga cgaagtcagt aaccatatac ggcaaggcga cgttgacgtg gtttgaagag
     9001 attttcgaag agtataagtc tctacataat aaaacgcata gtaccaaggt taacaaactt
     9061 gatactatgc gtttttcttt tttattcaat gtgggttgtt tgattagcaa aggcgataat
     9121 agcttgcttc aactcatctc cactgagagt gcggaactct ccaatctttt gattgatgga
     9181 ttctaaaggc atgacattaa ccttaccaac gaaaatctta tccataactt gattgtcaac
     9241 gagttcggtt gataccaaga gttcttcgta gagtttttca cctgggcgga ttccaacttc
     9301 aacgattgga atttcacttt cagtgtggcc acttagaagc accatcttct tggccaagtc
     9361 ataaatcttg actggtttgc ccatatcaag gataaagact tccccatctt tggcataagc
     9421 accagcatgg ataaccagac ggctagcttc tggaatggtc ataaagtaac gggtcatgcg
     9481 gaagtctgtc accgttacag gcccaccttc agcaatctga cgttcaaaga ctggaatgac
     9541 actaccacgg ctaccaagaa cattcccaaa acgaactgca cagtaggttg attggctacg
     9601 ttggttaaag ccagtgacaa tcaactccgc cacgcgcttg gttgctccca taacatttgg
     9661 tggattgact gccttatctg tcgaaatcat aaccatctta gacactttag cttcatcaac
     9721 agccttagca acattgtaag ttccacggat attgtttttg aaggcttctt ttggattgcg
     9781 ctccatcata ggaacatgct tgtgggctgc cgcatgataa acaatagcag gtttgtactg
     9841 ctcaaagact tgcaacaaac gatcatagtc ttgaatgtcc gcaatcacag gtacataatc
     9901 aatcccttgg aacttacgaa tcaattcatg ataaacaagg tagattgagt tttccccatg
     9961 accgagcaag acaatgcgtt caggattgaa gcgactaact tgacgacaga tttcagaacc
    10021 gattgaacct ccagctcctg tgactaagat ggtcttacct gtcagttctg cacccagacg
    10081 cgattcgtca agacggattt cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa
    10141 gccagtacct gcttggtgaa ggccctgaac aacagtttca accttaggca tcttgtaaca
    10201 tttgacaccc agcttattac acatctgcaa gatacgctca tattctgacg gatccagcga
    10261 cggaatcgca acgatgacac gctcgatttg atggcgtttg gctaattcag gcagattgtc
    10321 ataagagccc aaaacaggaa taccaccaag tttttgaccc tttttcttag aatccttatc
    10381 caaaataccg accagttcta attcactggt tggatgttgg taactatcca taaagagagc
    10441 cccaccatca ccggcaccaa tcaagaaggt ccgacggtgt tctccatcac cactaccttt
    10501 tttgcgtctg gagtagatta actgccaagt aatccgtggc aataaaatca agaaggtact
    10561 caacaagata aagagaatga taaaacggat ggagaagagt ggcaagaagg cataacagat
    10621 actatatgac aagacactgc tagcagtcac accaaaaaag attttcatga aatccgtaat
    10681 cttgctgtaa cgactaatgc tcgcgttcaa cccccaaaaa ccaatcatca attgatagaa
    10741 caggaaggcc aaactcgtat agataatgta gtcaacaggt gctggattaa tcagcccata
    10801 aaataaaata taagatacaa tgatggaaac caccatactg aaaatatcaa atattcccca
    10861 gaaaacctgc ttttgttgtt tatttaaaat ttccaccaga tcaatcacat aatctgttag
    10921 ttttttattc atagaaatct actcctcctt aaaagagttt tactatattg ttattatagc
    10981 atatttttat ttatttggct tattttatcc aaataaatta ttgttctctt gtactccttc
    11041 ttccatttaa atcctctaat taaacctaca atcgtcccaa agccataagc aaagtgaatg
    11101 gaaaataaaa gaaagggcat cacaattaga aatccatttt tatgttttaa taaagtcagc
    11161 aaagtgagta gtgacaaaag tagaaaatag gcacctaata gtaaagttat gaatacgaat
    11221 gtgatcggta acaatgctag actaaacaca agactcaaaa caaataaaca aggaacatag
    11281 tgaaataatg ataaacactt aaactgaaca tgacttgtca agccaatcca caaaccattt
    11341 aaatactttt gatgcagcat tttcttgaat gttggtcgaa tatactgata agatagaata
    11401 cttgggctat agcggatttt ataaccatat tctcgaattc tataatgaat atcattatct
    11461 tcagttcggc caagttgctc atttactaaa ccaaccttct gaaaaacctc tcgtttatac
    11521 attccatgaa aaatagaaga aacatatcta tcctcagaac tatttcgata attggcaatg
    11581 ctactgccaa acatattttc ctcaacaaga tgcaaggtct ctgcccattt tccttttcct
    11641 tcgacaatcg tcggtctagg ccccccacag acaaattcac cttgttgaat aatagccaca
    11701 ttgttcatta caaaagtctc agtaactttt gaatgagcat caatttttaa aataaggtcc
    11761 cctacagaat gtttaactcc caggttaaaa ccactagctt gatttttctt aggattgtta
    11821 tacaatctaa ttgagttaaa ctctgtatct tcctttataa attgctgaat gatagctgtg
    11881 gtcccatctg tggacatagc atttataaat agaatttcaa tattctcttt aggataggtt
    11941 tgatttttta agtcttcaat tagaccagac agatattttt cttcattgta tgcactgatt
    12001 acaatggata cgagaagact ttcaacattt tgagtcacta ctactctcct gaatttttcc
    12061 ttccaccatt ctatcataat tttcaagact ttcccatttt tatcttgaaa gccagaaacc
    12121 ttacttgaca ttatagtgaa aaagccttaa aataagctgt tattaaaatc agctagaaga
    12181 ggtattatat gaaaaagcaa tcactctttt ttgttccagg tattatcctg attggtgttt
    12241 ccttgcgaac tccttttact gttttaccca ttattttggg aaatatttcg caaggtctgg
    12301 aggtagaagt tagttcgctt ggtgtcttga ccagcctgcc tctccttatg tttaccctct
    12361 tttcaccatt ttctacccaa ctggctcaga aaatcggctt ggagcatctc ttcacctaca
    12421 gcctcttctt cttgaccatc ggctcactta ttcgactaat caatctgccc ctgctctatc
    12481 taggaacctt gatggttggg gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta
    12541 tccaagccaa tcaaccaaag aaaattggtt ttctgaccac cttatatgta acgtctatgg
    12601 ggattgcaac ggctctggct tcctatctag ctgtgcccat tacacaagcc agttcttgga
    12661 aaggacttat cctcctcctc acgttactct gtctagcaac ttttttggtc tggctcccaa
    12721 atcaccgcta taatcataga ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca
    12781 tgcgtaataa acaggtttgg gcaattatta tcttttcagg ttttcaatcc ttgatctttt
    12841 acaccgtcat gacctggtta cctaccatgt ctatccatgc aggtctatcc agtcacgaag
    12901 ctggcttgct gacttctatc ttatctctga ttagcattcc tttttcaatg accatcccaa
    12961 gcctgacaac cagtttatct actcgcaacc gtcagctcat gctcactctg gtttcactag
    13021 ctggtgtggt cggcatttcc atgctctttt tcccaatcaa taatttcatt tactggcttg
    13081 ccatccatct cctcatcgga accgcaacca gcgccctctt cccttatctc atggtcaact
    13141 tttcactcaa gacaagcgcc cctgaaaaga cagcccaatt gtccggccta tctcaaacag
    13201 gaggctatat cctagcagcc tttgggccaa ccctctttgg ttacagtttt gacctgttcc
    13261 actcttgggt accatctgta gctgccctct tgctcatcga tatcctgatg actgtggccc
    13321 tctttacagt ggacagagcg gataagatcc tttaaacttc tagttttagc tagaagtttt
    13381 ttatatataa aaattttaca catttctctt gacagggctt tcttttagat gtacaatgta
    13441 tgtgtagaaa aattatatat aaaaatccta cacattagaa aaggaggttt cccatgtact
    13501 ttccaacatc ctctgccttg attgaatttc tcatcttggc cgtactggaa aaaggggatt
    13561 cttatggtta tgagattagc caaaccatta agctgatcgc taatatcaaa gaatccacac
    13621 tctatcccat tctcaaaaaa ttggaaggca atagctttct gacaacctat tctagagatt
    13681 tccaaggtcg catgcgcaaa tactactcct tgacaaatgg tggtatagaa cagctcttga
    13741 ccctaaaaga tgaatgggca ctctatacag acaccatcaa tggcatcata gaagggagta
    13801 tccgccatga caagaactga atacctgact cagctagaac tctatctcaa aaaactgcct
    13861 gaagctgacc aaattgaagc catggactat ttcagggaac tctttgatga tgctggagtc
    13921 gaaggagagg aagaactcat cgctagcttg ggaactccca aagaagcagc ccacgaagtt
    13981 ctctctaatc ttcttgataa aaaaatcaat gaagcacctg ctcaaaaaaa taaccgacaa
    14041 attttacatg tcgccttatt agccctcctt gcagcaccca tcggtattcc tctgggaatc
    14101 gccatcctcg tgtccctgtt cgcaatcctt gtggcagcct tgactgtcat cttggctttc
    14161 tttgcggttt ccatactggg tatcatcggt ggattcctat ttttagttga aagtttcact
    14221 gtcctagccc aagccaaatc agcctttatc ttgattttcg gttctggttt actggctatc
    14281 ggtgcttctt cactagtctt actaggtatt tcctatgtag cccgcttctt cggtctactc
    14341 attgttcgct tggtgcaatt tgttcttaaa aaaggaaaga gaggtgacca gcatgcgtaa
    14401 attgacaaaa ggatttctca tttttggtgt cgtgactacc gttatcggct ttatcctgct
    14461 ctttgtaggt atccaatctg acgggattaa gagcctactt gccatgtcca aggagcctgt
    14521 ctatgatagt cggatggaag agttaacctt tggcaaggaa gtcgaaaact tagagatcac
    14581 tcttcaccag cacgcgctga ccatcacaga ctctttcgat gatcaaatcc acatttctta
    14641 ccatccatca ctttctgctc accatgatct tatcaccaat cagaacgata gaactctgag
    14701 tctcactgat aagaaactgt ctgaaagtcc atttatctct tctggaattg atgggattct
    14761 ccatattgta agtagttcat ctaatcgttt taatgatgtc atcatcggac taccgaaagg
    14821 gagaactcta aaagggatca acatctcagc caatcgcgga caaaccacca tcataaatgc
    14881 tagccttgaa aatgcgaccc tcaatacaaa cggctatatc ctccgaattg aaggaagtcg
    14941 tatcaaaaac agtaaactca caacgcccaa tatcgttaat atctttgata cagatcttac
    15001 agatagtcag ctagagtcaa cagaacatca cttccacgct gaaaatatcc aagtccatgg
    15061 taaggttgaa ctgactgcca aagattatct ccgaatcatc ctagaccaga aagagagcca
    15121 acgaattaat tgggatgtct caagtaacta tgggtctatc ttccaattca caagagaaga
    15181 gcctgaatca agaggtacgg aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa
    15241 ggatcaactc attgcgagat ctgatgatga tattgagcta atatccacac caagcagacg
    15301 ttgacaaaaa cgcttacatc tgctactatg gaaacatatt tatctaacca aaaggaggtt
    15361 ctaccatgaa acaagaatgg tttgaaagta atgattttgt aaaaacaaca agcaagaaca
    15421 agcctgaaga gcaagctcaa gaggttgcag acaaggctga agaaaggata gccgatctcg
    15481 atacaccaat tgaaaaaaat actcaagtag aggaggaagt ctctcaggct gaagtcgaac
    15541 tggaaagcca gcaagaagag aaaatggaaa ctcctgaaga cagtgaagcg agaacagaaa
    15601 tagaagaaaa gaaggcatct aattctactg aagaagagcc agacctttct aaagaaacag
    15661 aaaaagtcac tatagctgaa gagagccaag aagctcttcc tcagcaaaaa gcaaccacga
    15721 aagagcccct tcttatcagt aaatctttag aaagtcctta tatccccgac caagctccaa
    15781 aatctaggga taaatggaaa gagcaagtgc ttgatttttg gtcttggcta gtggaggctc
    15841 tcaaatctcc tacaagcaag cttgaaacaa gtagcacaca cagctacaca gcctttctct
    15901 tgctcattct gttttctgca tcttcctttt tctttagtat ctaccacatc aaacatgctt
    15961 actatggaca tatagcaact ataaacagcc acttccctga gcaactagca cctttaactc
    16021 tcttttctat catctctatc ctagtagcga caacactctt cttcttttca ttcctcttgg
    16081 gtagtttcgt cgtgagacga tttatctacc aagaaaagga ctggacgctg gaaaagattc
    16141 tccaacaata tagtcaactc ttggcaattc caatcttcct cactgccatt gctagtttct
    16201 ttgctttctt tgacagccta cgattttcag cccttttatg tgtgattagc attggaatca
    16261 ttctgcttgc tagtctccat atcattacca gaccaagtca agcaagtgaa gccgactcct
    16321 tctatcagtt attcttgtct gtccttgtga acggagttat tatcctcctc ttctttgtag
    16381 ctgaagtggc actgattgga aattatcttc gtatcttggc ctttctttaa acttcaatcc
    16441 tgtcatcctt ggcaggattt tttctatgtc aaaaaagcaa gtcgattgac ctgcttctgc
    16501 tttacttttc ttgtgctaat gttttaaaat cttgtcctag gatgggtttt attcctagag
    16561 ttcaaagtat gatagcgttg ctataaagtt cgttattcca aatattatgc aaactagtta
    16621 tatgtaaaat taagattaga aaccaccaat ctccatttat taacttttaa tgaaattcgt
    16681 ccatattaat aactttactt gctctagcaa taacatcctt gtcatgagtc acgacaataa
    16741 caatcttttc ctcgttcact aatttctcaa cctcgtttag aaataattca gttgagtttc
    16801 tgtccaaatt tcctgtaggc tcatcgaatg aatagatatc cttttcttgc gataaactcc
    16861 tcatgaatag aattttttgt ttctgacctt acgaaatatt ggtggctttc ccatcaatct
    16921 gatgactcag agctatattg agattcaaac tatcaagatt atttgattca tagttttcct
    16981 tatcaaatag ggtgaaatta atattttttg tca
//
LOCUS       DAVHIT010000040        16885 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVHIT010000040 DAVHIT010000000
VERSION     DAVHIT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.041"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(68..1390)
                     /locus_tag="VVN16_001175"
     CDS             complement(68..1390)
                     /locus_tag="VVN16_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU5572571.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1481..1569)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1733..4069)
                     /locus_tag="VVN16_001176"
     CDS             complement(1733..4069)
                     /locus_tag="VVN16_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU5572572.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKVKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQIGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4173..4721)
                     /locus_tag="VVN16_001177"
     CDS             complement(4173..4721)
                     /locus_tag="VVN16_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU5572573.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4718..5020)
                     /gene="zapA"
                     /locus_tag="VVN16_001178"
     CDS             complement(4718..5020)
                     /gene="zapA"
                     /locus_tag="VVN16_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU5572574.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5107..5988
                     /locus_tag="VVN16_001179"
     CDS             5107..5988
                     /locus_tag="VVN16_001179"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU5572575.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQITPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5992..6606
                     /gene="lepB"
                     /locus_tag="VVN16_001180"
     CDS             5992..6606
                     /gene="lepB"
                     /locus_tag="VVN16_001180"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5572576.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6680..6808
                     /locus_tag="VVN16_001181"
                     /pseudo
     CDS             6680..6808
                     /locus_tag="VVN16_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
     gene            6885..9251
                     /locus_tag="VVN16_001182"
     CDS             6885..9251
                     /locus_tag="VVN16_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU5572577.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGEQKRQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDTDFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDTEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDHATQHIGTARKTYLVERFSDLLKN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(9299..10582)
                     /locus_tag="VVN16_001183"
     CDS             complement(9299..10582)
                     /locus_tag="VVN16_001183"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU5572578.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(11381..12517)
                     /locus_tag="VVN16_001184"
     CDS             complement(11381..12517)
                     /locus_tag="VVN16_001184"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU5572579.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIDQIVEYI"
     gene            complement(12579..13016)
                     /locus_tag="VVN16_001185"
     CDS             complement(12579..13016)
                     /locus_tag="VVN16_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5572580.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYESGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(13018..14973)
                     /locus_tag="VVN16_001186"
     CDS             complement(13018..14973)
                     /locus_tag="VVN16_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU5572581.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(14997..16766)
                     /locus_tag="VVN16_001187"
     CDS             complement(14997..16766)
                     /locus_tag="VVN16_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU5572582.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHINVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKILELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT         5043 a         3698 c         3210 g         4934 t
ORIGIN      
        1 tatattatat atcaggctga taaaaattat ttataggcaa aaaaatcaca cgagctgtgt
       61 gattccatta tttgtcaaaa tactttttag tttcagcaat aacgactggc gacaagacca
      121 agagggcaat caagtttggc agagccatca aggcgttaac gatatctgcg ataatccaga
      181 ccatatccaa ctcgataaat cctcctaaca agaccatgag cacaaaaacc acacggtaga
      241 gccagataaa gcgaacccca aagaggaact caaaacagcg ttctccgtaa tagttccaac
      301 ctagaatcgt tgtaaaggca aaaagtacaa ggaagatggt caagagggca ggcccaaagt
      361 gtgaaaagac tgttgagaaa gctgactgag tcaaggcaac cccattcaag tcaccactcc
      421 aaactccagt taccaagatg gtcaaaccag ttagagtaca aatgatgagg gtatcaataa
      481 aggttcctgt catggaaatc aaaccttgct ctactggttc atttgtcttg gcagctgcag
      541 ctgcaatagg agcagaaccc agaccagatt cgtttgagaa cacaccacgc gccacaccat
      601 tttgaatagc catccgaacg ctagcaccag caaatccacc taccgcagca aggggactaa
      661 aagctgaggt aaagactaaa gcgattgtgc cagggatttt tccaatatta aagaaaataa
      721 ctgtaagagt tcctaagata taaatgatgg ccataaaagg aacaacagta gttgaaacct
      781 tagaaataga cttgagtcca ccaaagactg caatcgctac aaagacagac aagacgagag
      841 ctgtgatggc tggcgaaatc gtcgttgtat tttggataga ttctgcaatc gagttgactt
      901 gggtgaaggt tccgattccc aagagagcaa ccaatactcc tgctactgca aacaaaacag
      961 caagtggtcg ccacttttct cccatcccta gaaggatata atgcatggga cctcccgcta
     1021 ctgcaccatg gtcgtccttg gtgcggtatt tgatggccaa gagtccttcc gcatacttgg
     1081 tagccattcc aaagaaagcc gccatccaca tccaaaatag agctcctggt ccaccaacct
     1141 tgatagccgt cgcaactcct atgatatttc ctgttccaac agttgatgcc aaggctgtac
     1201 acagagctgc aaaactggat acatcaccat gtcccttatc ctggataaaa ataagctgaa
     1261 aagccttggg cagacgcaaa acctgcaaga gtcctagccg aatagttagg taaatccctg
     1321 ttccgaccaa taaaatcaag aggggcggtc cccaagcaaa agcatcgatt gatttaagca
     1381 attctaacat ttccttctcc tatcgtttca accccaaaag aaagagcaca tgcaagatac
     1441 atgtactctg gaatgcttag ataaatgcta aaaagcggtc tatcctagct ctgtcctttt
     1501 acctgagagt ttgagcagtt gcctgccttg ccccttcggt gcctttacgg tctctccaga
     1561 gttccgtcca tttacagtca tggaaaatca aacgattccc cacttctatt aaacttcatt
     1621 cggtgttggt atttaattga ttctaatttt acaaaaaatg ttggcttttg tcaatgtgtt
     1681 tattagtaaa aattagttca acagttttta ctttataaag tccagaatac tgctatcctt
     1741 taaaagtgac aatagtcgca ccactgcctc cagcattttg tggggcatag ccgaaactct
     1801 tgacatgttt gtttctttgc aagtatttgg taactccttc acggatgact cctgttccga
     1861 taccatggat gatatcaact tgagccatat tgttaagcaa ggcttggtcg atgaaggtat
     1921 ctagctcatt catggcttct tcatagcgct tgcctcgaag atccagtcta gcttgaggtc
     1981 ctcgcccaga agttcgtttc acaacattga cctgtttctt cttgactggt ttttcttgct
     2041 gggcttgaac aagatcaaac tctttctctt ccaaggtcat cttaatcaag ccaatttggg
     2101 cttcccagcg gccgtccttg agttgactgg tcaaggtacc acgctgacca taactgagaa
     2161 ccacgatatc atctcccacc tttggagctc gttttttctt ggccttttga aggaccttgt
     2221 ttttagacaa gtccactttt tcaggagcca attttttcaa cttgaccttg gcttcaatga
     2281 tttcgtgggg cttgagttgg gatttactgt ggagattttt gagaatctgg tcactttcac
     2341 ttagggccat atccacaatc tcagcagcct gttcacgcgc cttgttaagc tcggtttcct
     2401 tttcacgatt aagctcgttg tagagttttt ttagcgcacg gttcatcttg agattttctt
     2461 gctccacctc acggatattg tccaagcgtt tgcggctttc cagcgtctgc tcttctaatt
     2521 gctcaatgat acgattgacg tcattgtcct gatcgatctg ctgactggca tctcctacga
     2581 taacttcaga taggcctaga cgtttggcaa tttcaaaggc attacttcgg ccaggaacac
     2641 cctgcataaa gcgataggtc gggcgaagag ttgcagtatc aaactccata ctggcatttt
     2701 gcacaaaggc tgtctcaata ccgtaggcct tgagttctgg atagtgggtc gtcgccatgg
     2761 tcttgatttg acgcaggcga aggtcctcca gaatagccat ggcaagggct gctccctctt
     2821 ggggatcagt accagccccc aactcatcca aaagtaagag tgaatgttgg ttgaccttgc
     2881 caagaatatc cacgatattg gtcatatggc tagagaaggt agacaagctc tgctcaatag
     2941 actgctcatc tccaatatca gcaaagattt cttcaaaaat accaacacga cttcccttgt
     3001 ctgctaaaat cggcaatcct gactgggcca tgacctgtgt caagcccaga gttttaagca
     3061 tgatggtctt cccacctgta ttgggacctg taatgacaat agctgttaaa tcttgaccaa
     3121 aatagacatc atttgcgacg gcatttttga ccaaaggatg gcagacatgg agcagttgaa
     3181 tctcttgatt ttctgacagc tgaggcacga ctgcttgtct ttcttgaata aatcgaacct
     3241 tggcacgaat caagtccaga tgaccgataa tccaagcgtc attagcaatc tcagccgcat
     3301 gagggcggac acgctcagaa atttcttgga gaatgcgaag catttcatag cgctcatctg
     3361 ctcgcagact agcaatttct tcgctcagtt tgactacctc acgtggttcg atatagacgg
     3421 tgtttccact agcagaaata tcatgaacga cacctgcaat cttattgcgg taggtgtttt
     3481 tgactggtaa aacctgacgg ccatttctgc tagcaacaat tccttccgtc aacatctgcg
     3541 ctttttgctt gagcaagtct tgtaaaacat cgcgtacctg actctcgcta tcatgtattt
     3601 ttcgacggat tcgcgccaat tcttcactgg caaaattttc aatgaaaccc gcatcattaa
     3661 aggcctgaag atttccttgt aattgcggaa aatcatgtaa tttctcaaac caaagggcta
     3721 attcttccaa gctgacattt tccagattgg cgtaaaaact ttgaagttct cggctggcaa
     3781 gaagcacgcg tttcaagagt aggaactcct cgatattgag atccgctccc atctccaacc
     3841 tcttgcagac tcctgcaatt tccttagttg agagaatagt aaaatgcggt tgctcgacga
     3901 aaagagcctg catttctttc atctcagcaa aagcctgttt gattttatct gctttggcag
     3961 tcggagccag ctgtctcaat tgctccaagc cctgctcggt caacaaatga ggctcaaaca
     4021 aggccttgac cttatcgaac tctaatgttt ctaatatttt cttattcatc tttatttcct
     4081 tgtctttgaa tgtctaggtg tggtagcttt ttacattctg atagattcga gtcaaatctg
     4141 taaacaaagg gctaggaaaa ctcctgccct ttttatccga ttaaatttgt cacccagatt
     4201 tgtttgagcc aactggttgt tatcggtatg ctctggatga tgtgttttgc gacgatactc
     4261 ttttcaagag gattttgtat aactgccata gggatggtcg ccaagattgt caaggccatt
     4321 tgcaagacaa ataaggtcac caacatggac aagatacctg ctgaaacttg gaacaactta
     4381 ccacccagtt ttttgctagg aatcaagtgt aagagaagac caagcaaacg accaatgcta
     4441 tagacaatcc caaatacaag caagtagccg atacctgcat aaaagacctt atccagctga
     4501 aagagttgat ccgatgggaa aaagaaagtc ccctgacctt cctgcgaatt tgcataaggg
     4561 agcaataaat ggaattgctc tccaagcccc ttataaaact ggccagccat aaaagccgat
     4621 gccatggctg aaatcaggta ataaacctgt aagagcaggc ctctccgata gccgatataa
     4681 aatccccaaa ccaagaccaa tagaagaagg aatgaaatca taaggaatcc tcaatcttgc
     4741 tctgttcttg cttacaagtc acaagcttgt gacggagttc ttctagctct tgctccttat
     4801 cgtcaaattc aatctcacgg ctgagctgag ttgataaaca gttgactgcc aacaaaagag
     4861 cgattgtttc atcatctgcg ctaggcattt gttctttaat tgcttggtat ttttctgtcg
     4921 caaccttagc gatttcctcc ataaaaaggt tatcatgttc gcttgtcaag gttaacgttt
     4981 ttttcccgaa tgtaaatttg aatcgattta gatttgccat aaaattcacc tcacgatatt
     5041 ataccaaaat tcgctaattt tgtcagtttt tacaaatttt actgcttttg tggtacaata
     5101 gaaactatgg caagtatcac actcacacca agcgaaaagg atattcaggc ttttcttgaa
     5161 cactatcaaa ccagtctggc tcctagcaag aatccctata tccgctactt tttgaaacta
     5221 cctcaagcaa cggtttctat ctatacttct ggaaaaatct tgcttcaggg tgaaggggct
     5281 gaaaaatacg ccagtttctt tggctatcaa gctgtagagc aaaccagcgg acaaaatctt
     5341 cctttaattg ggacagatga ggtgggaaat ggttcctact ttggtgggct tgcagttgtg
     5401 gctgcctttg tcacacctga ccagcacgac tttttacgaa aactcggtgt gggggattct
     5461 aagactctga ccgaccaaaa gatccgtcag attactccta ttctcaagga aaaaattcag
     5521 caccaggcac tccttctctc acccagcaag tacaacgagg tcatcggaga ccgctacaac
     5581 gctgtttcgg ttaaggttgc cctccataat caggctatct atctcctcct tcaaaaaggt
     5641 gttcagcctg agaaaattgt gattgatgcc tttaccagtg ctaaaaatta tgacaagtac
     5701 ttggcacaag aggccaatcg tttcagcaat cctatcagct tagaagaaaa ggctgagggc
     5761 aaatacttgt ctgtcgcagt ttcttctgtc attgcgcgtg atctctttct ggaaaatctt
     5821 gaaaatttgg gacgagaact gggttatcag ctcccaagtg gagctggaac ggcttctgac
     5881 aaggtggcta gccagatttt gcaagcctat ggtatgcagg gactcagctt ctgcgccaaa
     5941 ttgcacttta aaaacactga aaaagcgaaa aaacgcttag aaaggtaagt tatgaattca
     6001 tttaaaaatt tcttaaaaga gtggggattg ttcctcctaa ttctgtcatt actagcttta
     6061 agtcgtatct ttttttggag caatgttcgc gtagaaggac attccatgga tccgacccta
     6121 gcggatggcg aaattctctt cgttgtaaag cacctcccta ttgaccgttt tgatatcgtg
     6181 gtggcccatg aggaagatgg caataaggac atcgtcaagc gcgtgattgg aatgcctggc
     6241 gacaccattc gttacgaaaa tgataaactc tacatcaatg acaaagaaac ggacgagcct
     6301 tatctagcag actatatcaa acgcttcaag gatgacaaac tccaaagcac ttactcaggc
     6361 aagggctttg aaggaaataa aggaactttc tttagaagta tcgctcaaaa agcccaagcc
     6421 ttcacagttg atgtcaacta caacaccaac tttagcttta ctgttccaga aggagaatac
     6481 cttctcctcg gagatgaccg cttggtttcg agcgacagcc gccacgtagg taccttcaaa
     6541 gcaaaagata tcacagggga agctaaattc cgcttctggc caatcacccg tatcggaaca
     6601 ttttaagaaa cctaagaggc cgagaatcac caatctcagc ctcttcttct atcgtgagaa
     6661 gtgattgttt atttacaaat actcaatgaa aatcaaagaa caaactagga agctagccgt
     6721 aggctgctta aaacagtgtt ttgaggttgt ggatagaact gacgaagtca gctcaaaata
     6781 ctgttttgag gttgcagatg gaagctgacg tggtttgaag atattttcga agagtataaa
     6841 ataaaaacaa tcccaactct cacctattca tgcaaaggaa ttttatggaa gtttattttt
     6901 caggaaccat tgaacggatt atttttgaaa atcccagcaa tttttatcgc atcctcctcc
     6961 tagaaatcga cgatacggac gcagaggatt ttgatgactt tgaaatcatt gtcacaggaa
     7021 ccatggctga tgtaattgag ggcgaagact atactttttg ggggcaaatt gtccagcact
     7081 ccaagtatgg agaacaactg caaatcagtc gttatgatcg cgcaaaacca actagtaagg
     7141 gcttggtcaa gtacttttca agtagccatt tcaaggggat tggtctcaag acagctcaga
     7201 aaatcgtaga tacctatggc gaaaatacca ttgatgaaat tttgcaacac ccagaaaagt
     7261 tagaaggcat cgcaggactc tctgccaaaa atcgcgaggc tttcgtctcc actctccgtc
     7321 tcaactacgg aacggagatg gttttggcca aactagccaa ctacggcatt cccaacaaac
     7381 tagcctttca gattcaagac ttttacaagg aagaaaccct tgatgtggtt gaaaattatc
     7441 cctaccagct agttgaggat atcaagggtt tgggctttac cattgctgac caactagctg
     7501 aggaactagg catcgaaagt caggctcctg aacgcttccg cgccggtcta gttcacagtc
     7561 tttttcaggc ctgtatggaa acaggggaca cctatgttga agcacgggat ttgctggaac
     7621 aaacccttac tctccttgag tcttcccgtc ccgtggaact agaccccagc caagttgccc
     7681 aagagctctc ctacctgatc gaagaagaca aggttcagca gattgatacc aagatctttg
     7741 acaacagcct ctttttcgct gaggaaggta tccgcagtca cttgattcgt atccttgaaa
     7801 aaggagaaca gaagagacaa gatttagaaa ctattcaaaa gcatatcact actgtcgagc
     7861 aagaactggg gattgagtat gataacattc aaaaacaagc tatttgtgac gctatccaga
     7921 acaaggtctt tatcctgaca ggtggacctg gcactggtaa gacaactgtt atcaatggga
     7981 tcatcgctgt ttacgccctg ttagagggac ttgacctcag gaagaaaagc aacctgccca
     8041 ttcttcttgc tgctccaact ggacgagccg ctcgtcgcat gaatgaattg acaggcttgc
     8101 ctagcgcgac catacaccgc cacttgggaa tgacaggtga cgatgatacc agtcatctgg
     8161 aagattacct agatactgac tttatcatcg tggatgaatt ctctatggtg gatacttggc
     8221 tggccaatca actcttctcc aatatctctt ctaacagtaa gatcctcatc gtaggtgaca
     8281 gtgatcagct accttctgtc agtcctggac aggttctagc ggatctgctt catattcctt
     8341 tgattcctca gactcgcttg gaaaaaattt atcgacaaag cgaagaatca accatcgtca
     8401 ccctagctag tcagattcga cagggcatct tgccagctga tttcacccaa aaaaaagctg
     8461 accgttccta ctttgaaatt gctagtggcc atattcctgc caccattgaa aaaatcttag
     8521 gtgccgccct cagaaatggt attcctgccc gtgatatcca agttctggct cctatgtacc
     8581 gagggacggc agggattgat gctatcaatc agctcatgca agacctgctc aatcccccac
     8641 aaaaagatca actcagtttt gaagctcctc agtgccacta tcgtaaggga gacaaggtca
     8701 ttcatttggt taacgatact gaaatcaatg tctttaatgg ggatttagga gctatcacag
     8761 acctgattcc tggtaaatac accgaatcga aacaagacga gattgtcatt gattttgatg
     8821 gtaatgaagt ctcttacccc cgtaacgaat ggtacaagat tcgcttggcc tatgccatga
     8881 gtattcataa gtctcaggga agtgagttcc cagttgtcat cctaccgatt actagtgcta
     8941 gccgacgtat gctggagcga aatctcatct acacagccat tacacgtgcc aaaagcaaac
     9001 tcatcttact aggcgaatta caagccttcg accatgctac ccaacacatc ggaactgccc
     9061 gaaaaaccta tctggttgaa cgcttcagtg atctactgaa gaatgttgaa gaaaagcaac
     9121 aagctgtctc tgaaacagtc acatcaagtg cctctgaaca atcctacatc ctaaccgaag
     9181 aaaactggga ccgcatccca gccatgattg ggattacaga cacagacctc aaagagattt
     9241 ttggaaaata gtgcatcaga aaacccacct gaattggtgg gttttctgtt tattaagatt
     9301 attttactgt tgctgtgctt gtgatcaatt caacagcttt tttgatagtg atatcatgtt
     9361 tcaacatgtc agctgaaagc aagttttgaa cttgtgcaac ttccatgttg tagtctgctg
     9421 ccaattgctc aacttctttt tggatttctt cttctgaagc atcaaatcct tcagctttgg
     9481 caactgcttc gataacaagg ttagtcttag tacgtgactc agcttctgct tggtattggt
     9541 tgtgaaggtc ttcttgagta gttccagtga tttggaagta catgtcaggg ttgatccctt
     9601 gacgttgcaa gttcccaagg aattcattta ctgaacggtg aacttcttca tggatcattt
     9661 cttctggaag ttctacgatt tcagcatttt ctacagctgt atcaattgct gcaccttcaa
     9721 ctgcatcttt gtaagcttct tctttagcag cagccaattc tttgcggtat ttttctttca
     9781 agtcagcaag tgtttcaact tcttcatcaa tgtcttttgc aagttcatcg tcaagagctg
     9841 gaacttcttt agcttttact tcgtggatag ttgtcacgaa tttagcttct ttacctgcaa
     9901 ggtcttctgc ttggtagtct tctgggaatg ttacgataac atcaacggtt tcgccagctg
     9961 agtgacctac caattggtct tcgaaaccag ggatgaattg acctgaacca agtccaagtg
    10021 agaagttttc accttttcca ccgtcaaatt caacaccgtc gatagaacca acgaagtcga
    10081 tcacaacagt gtcgccgttt tcagcagcag cttccttgat aaccaattca gccaggttgt
    10141 tgcgttcgcg ttcgatacgc tcttcaacat cagcgtcagt tacttctttt tctacatcaa
    10201 ctgatacttc aaggtttttg tagtcaccca attttacttc aggttttgta acgacttcag
    10261 cagcgataac ccagtcttga cctttttcca ttgaagttac gtcaattttt ggttgggcaa
    10321 ccacttcaag accagcttct tttacagctg cttcataagc gtttggcaaa agagcgttca
    10381 taacgtcttg gtaaagtgat tcttcaccaa attttttatc gaagatagga cgtggaaggt
    10441 gacctttacg gaaacctgga acattaagag atttcttcac tgacttgaag acacggtcca
    10501 attctggttt gatttggtct tgagagatag tgaaagtcaa gacaccacgg tttgtttctt
    10561 tgttttcaaa tgatacagac attctgtcat ttctccttaa aattttttaa atacagtcta
    10621 ttataacatt ttctataaat aaaatctaga ctaatgcagc caattataga caacaagcct
    10681 ttttcctaac taaaacactc ataaagtata gacaatttta gactactttg gatatctttt
    10741 ggttagtcat tttccaaaac taatttatct aatttttcag ttatatagta gattgaaact
    10801 agaatagtac acctctactt ctaaaacatt tttagaaatc gatttgactg tcctgatcga
    10861 tttgtcctgt tcttgtttca ttttactata tagatcgcat tcgaaagaat tcgttccaaa
    10921 attgcttggt tcgttcttct cttggacgac taaagaattg atgggcatta tttttttcaa
    10981 caattttccc acagtccata aaaataacat gatgggctac atctctagca aaacctatct
    11041 cataaagctg atcaaaaact caatctgagg agaaaatcca ataaggcttt ccataataaa
    11101 ggacatccta tcaagatttt tgaacacccg ataggctgcc tttttccgat tttgaagact
    11161 ttttgtccag cttcttttac cttataaata acaacaaaaa tcaaagacaa gctagaaaga
    11221 ttaccgcaga taactcaaac agtgttttga ggttgcagat aagactgacg gagtcagctc
    11281 aaagcactgc tttgaggttg tagatagaac tgacgaagtc agtaacatat gcctaagaca
    11341 aagcgatgct aacctgattt gaagagattt tcgaaaaata ttaaatatac tctacaattt
    11401 gatcaatcaa ttcttggtcg tctaaacctg taacttgtat aacaacatct ttgactgatt
    11461 gtttagccaa caattcacct aatcgaatac tttcttcatc atttacatcg cgatagtcaa
    11521 aggcatagcc aactgtttta agtaggtttt tatatgacaa actgagttct ttcaattcac
    11581 gtatcggccg gatgaatcgt tcattatagc ctaattttcg gattggagta cgagctacgc
    11641 gactaacctc gtccactatg aaagggtttt caaaccgttc tataatgact ttgtgataat
    11701 tctccaattc ttttttatca aagttccatt tggcaatcaa gagactccga atttcagcta
    11761 atacagattc aatccgagat ttgatattag gattttgaag agcttccaaa attgtcttgg
    11821 caccataatg cgcaccaatg taagctgaag ttgcatgtcc agaattgact gaaaaaagtt
    11881 ttcgctcaat aaagggttct aaatcttctt cataatgcac atcttttaga cgtaaatctg
    11941 gatttttaag acgcttggtt tccacgaccc attcattaaa gggctcgacc acaacaaaaa
    12001 gggaatcttc gtgactttgt gctggaacaa tcctgtctac tgcagcattt ggaaaaccta
    12061 tgtagttatc agcaaatgtc aaaccttccg gacttaaata tttcttgact tcttgataaa
    12121 gaaattgaga cccgccaatc atattttcac aggccataac atccaatacc tgtgtatttc
    12181 ctgcaactcg gcgagcttcg attcctttgg ctagaagttc agcgataaaa gggagtatat
    12241 taggtccgat tgcagtagta ataatatccg tcttttgaat cgcttcaatg acttgctcag
    12301 gatgttcttt gctattaatg ccagccacat tagttacttc tatacgagac tgtcctttct
    12361 gtgcaatttc aatttcatac ttgccctttt catttagagc atgaattatc tgattattga
    12421 catccacaaa atcaatatgg aaaccatttt taaatagaat ttcacctata aaaccacgac
    12481 cgatattacc ggcaccaaaa tgaacagaat gtttcattca atttctcctt ttcctatttg
    12541 agagacatat atactagatg acaaatgacg aaattcaatt attcaacagc atcaaataag
    12601 cgcaatactt cctcttttga ctgagcatct gctagtttaa gaacattatc tacatctgca
    12661 cagaagatag aaattttctg aataatttct aagtgttcat taccaatacc agcaatacca
    12721 aaaagaaccg ttgccacttg agggttagat acattcccaa aatcaacccc tctagggact
    12781 tgaacgactg taataccaga cttgaggaca tccttttttg ctgcatctgt tccatgcggt
    12841 atggcgataa agttacccat gtaaacagat agctctttat ctcgctcaat catggcttca
    12901 atatagtctt cattaacata tccactctca taaagaactt gcccacaata acaaatagct
    12961 tcctccttgt tagaaaattg tttattaagc ttaatcaaat gtttttcaag tttcatatta
    13021 gttctccaat ttcttaattt tttcgttaaa aatagtattt agcatctgat aaataatgga
    13081 ttgatttccc gtcttgtaaa tctctgtata aagatgattt tcaataatcg actgactgat
    13141 agctgtcatt aaatctctcg cttcctcgct agcttcttta gacattagca ttagcagaca
    13201 ccttttgacc tcttcttcct ctcgtttcat tgacaatgcg gagataggta gtttgagttc
    13261 aaacatagta aaactatttg ttgttacttt gctagactga ctatgtaaaa gcaccagacc
    13321 agtattagga atagccatag gactattctg gaagtgagat aacaattttc ttgttaggta
    13381 atctctgtca ctcacattct tctgatattg gatgatttgt tctaccgtgt gctcaaatga
    13441 ttgattattc tccaattgaa ccaactcgaa ttgactcaaa agctgactac tagatattaa
    13501 ataatcttgc aagtcataac ttttctctgg agcgatagca tcacgcgcga caattgtacg
    13561 atgtgcctgt acatactgaa gtcgttcctg taattcaagc aattctttga cagttggaaa
    13621 actagaaatt acatcgattt cctgattagt cagcggcttg gtagataaaa tataatcata
    13681 ctgactgaga tcaatactgt ggtaatctac tagagactga atatctatca actctacaaa
    13741 aggagcaaca ctcttaattt tactgactaa taaatctcta gtgacccgac gttcatccgt
    13801 taaaagcaaa acacggactg gataaatctc tggactacgg cctaagctag agataaaatg
    13861 tagggcaatc atgccatact catactctgt atgaagatat ttcggaaaaa tatcattcac
    13921 taaaagactg atgactgtat aaagaaattt attcctttca actaaaatct ggacagattc
    13981 tggcaaattt tcaccctgaa aaaggatagg gacgcctaaa ctgagccgaa tatgatggaa
    14041 aagaaaattg aataaaacct tgtccttaaa aaagtcaatc ttggtataca tggaaaccgt
    14101 atcaatcaga tttgaaatat tgtagaaaaa ttcaccatca aatttctccg taaagagcgg
    14161 attgtcctga cgtttaatga ttaattcatc caagatgctc gcaaaataga taatttcctg
    14221 aatactataa aattgtgcag tctgcttaga gtaagcttga aaaatttttt gagaaatttc
    14281 caaagcctgc ttactagtat taggtgaatt ttcaatgttt tgctcctgac ctataagaga
    14341 taacaagatc gcaaaaaaca tcttcatcct agcatccata tctggaaaac ctgacagttg
    14401 cttattaaca atctgactgg ccagcctagt tctatctgct tctaaaatat caaagctccc
    14461 aaaattaccg gttgaaaaat ctgctactga gatacagttt gtcagtaaaa tagccaaaaa
    14521 tcttctctta ccaactgaat caccagaaat ccgataacct ttttgtcgtt caattttcag
    14581 atcaaagtct aaaagacgct tatcaatatc tgaaatatcc tgaataatag ttacattact
    14641 gactttcgtg cactcttgca atgcttcatt ggtgacaaaa ccactctcag tcagaaggcg
    14701 ataggtaatc aactcttgtc tttcttgggg actatactca actaacactt caagttctgt
    14761 cggcaaatca tccaactctc cagtcaaaat atagtatttc ccatgcttcg ttatttcgat
    14821 tccatattgc tccatgctat ctgtcaaatc tgataaagtt cgataaattg ttctagatga
    14881 aacctgtaag atttcagtca tatcttgcat tgaaagcttc cctacatgta ggaaagcctt
    14941 caataattgt tcttctcgtt tggttaatag cattcgtaat ttagaaacct ctagttctat
    15001 ttgtacattc tagcagccat tttgtcatat tctggtgttg ttactaaact atccacaata
    15061 agaaattgag aattcggtgc tgcttgctgc acttctgcct gtaaagaaat agttgttaca
    15121 atcattatgt ttttagaatt aaattcacct aattctaaaa ttttggcagt agaaactgga
    15181 atacgaatat tcttgtttct aaagatagcc cgaatcgttt cacagcccat agttgcagtt
    15241 ccctgtgctt taccataagc aactactaca gcatcaatat ggttaaggtc aatttcctga
    15301 ctatctactg gtgctgaagt tggtatatct ccttcaattt ctgctattgg agaagctcct
    15361 gttaatgaag ctacaatttc atcataacga ggggacgcta agaaattatc aacagaaaca
    15421 tgaatagcac ttggactctt gtctttagct cttggtgtca gttcttcctg agtaacaatt
    15481 aatgtttttg gtgtatcaag caaatttgag attgcctgat tagatactgg aatctctaga
    15541 cctgcttttt taaccttatc tcgaagaata ctagctccca tagcagagct tcccatacca
    15601 gcatcgcagg cgaaaatgat tttttccact gagtctgtcg aaacaactgc atcaacagaa
    15661 gttgatacta actgaccttt agactgagcc ttagctgctt gggtagccgc ctgagcagct
    15721 tcgagcgaat cctcagttga cttgtctgca tgaagaataa gggctgctac aaggaaagaa
    15781 acaactgctg ctactaaaac acctagaaga acatttatat ggggccaaac accttttggc
    15841 gccgtagcca taatcgcaat aatagaacct ggtgaagctg gagatttaag accagcgtct
    15901 aagagttgaa aagtaaaagt tccagagata cctcccgcca tagcagctaa aaatagagta
    15961 ggcttcatca taacataagg aaagtaaatt tcatgaatcc ctccgaagaa atgaataacc
    16021 attgcccccc aagaagaaga tttagcagaa cctttaccga atacagcata agctaataga
    16081 attcccaggc ctggtccagg attagcttcc aataggaaga gaattgactt accagcttga
    16141 gctacctgtt ctactcccag aggagtaaaa atgccatgat tgagggcatt attgaggaaa
    16201 aggactttag ccggttcgat gataatatta gccataggaa ggaggcgagc attgacaata
    16261 gcctcaacac cattcccaac agctccagta agagtcgata cgactggacc gattgcgtag
    16321 aaagccaaaa gcaataatgc aaaaccaacg agaccagctg agaagttatt aactaacatt
    16381 tcaaatccgg gacgaatttt ttcttggaac ttctcatcaa atttcttgat agtccatcct
    16441 cccagtgggc ccattaccat agctccgata aacataggaa cactagaacc tgtgattgca
    16501 ccaacagtag caatagctcc tacaacggca ccacgttggc catggatcat atatccacct
    16561 gtgtaaccaa tcaggattgg caataaatac gttaacatag gaccaacaac agtagctaac
    16621 tgttcatttg gcagatagcc atcagcgata aagagggcag tcaatactcc ccaagcaata
    16681 aatgctccaa tattgggcat aaccatattt gaaagcgatg tccctagttt ttgaaccctg
    16741 actttcaatg atactttttc ttccattaga tcatcctcct tttcttaaaa ctagtatata
    16801 caaagttaaa aacctttaaa actaaagttt tgtcacagat tttgtgtcaa aacattactt
    16861 ttgacgaggc tgggcaaaaa ggctt
//
LOCUS       DAVHIT010000041        16693 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVHIT010000041 DAVHIT010000000
VERSION     DAVHIT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.042"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1399
                     /locus_tag="VVN16_001188"
     CDS             <1..1399
                     /locus_tag="VVN16_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5572583.1"
                     /translation="SELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNGIRIINTDD
                     SDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRIISQINKVIKR
                     QFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQLLELVYKET
                     SFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEGVAQSFE
                     SEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQISVK
                     YQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDIKKEL
                     SHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFVA
                     ETLSYYCSNNFELEVWTELELSKESLEDSSYDIIISNFIIPPIENKRLIYSNNINTVS
                     LIYLLNAMMFIRLDE"
     gene            1760..2761
                     /locus_tag="VVN16_001189"
     CDS             1760..2761
                     /locus_tag="VVN16_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5572584.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            2911..3828
                     /locus_tag="VVN16_001190"
     CDS             2911..3828
                     /locus_tag="VVN16_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5572585.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3839..4729
                     /locus_tag="VVN16_001191"
                     /pseudo
     CDS             3839..4729
                     /locus_tag="VVN16_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            4758..6374
                     /locus_tag="VVN16_001192"
     CDS             4758..6374
                     /locus_tag="VVN16_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5572586.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLSLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFTFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6384..7703
                     /locus_tag="VVN16_001193"
     CDS             6384..7703
                     /locus_tag="VVN16_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU5572587.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVAFAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(7911..7983)
                     /locus_tag="VVN16_001194"
     tRNA            complement(7911..7983)
                     /locus_tag="VVN16_001194"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7948..7950),aa:Lys,seq:ctt)
     gene            complement(8248..9519)
                     /locus_tag="VVN16_001195"
     CDS             complement(8248..9519)
                     /locus_tag="VVN16_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU5572588.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFNFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9817..10032
                     /locus_tag="VVN16_001196"
     CDS             9817..10032
                     /locus_tag="VVN16_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572589.1"
                     /translation="MMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEILD
                     LYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10197..10376
                     /locus_tag="VVN16_001197"
     CDS             10197..10376
                     /locus_tag="VVN16_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572590.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     ADDELLDKEAVFTTH"
     gene            10513..10692
                     /locus_tag="VVN16_001198"
     CDS             10513..10692
                     /locus_tag="VVN16_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU5572591.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITILHGK
                     LNKYTERGIRKQAGL"
     gene            10729..11181
                     /locus_tag="VVN16_001199"
     CDS             10729..11181
                     /locus_tag="VVN16_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU5572592.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11472..11942
                     /locus_tag="VVN16_001200"
     CDS             11472..11942
                     /locus_tag="VVN16_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU5572593.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            11961..13049
                     /locus_tag="VVN16_001201"
     CDS             11961..13049
                     /locus_tag="VVN16_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5572594.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13030..13188
                     /locus_tag="VVN16_001202"
     CDS             13030..13188
                     /locus_tag="VVN16_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEU5572595.1"
                     /translation="MELEISDFLGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            13298..14332
                     /locus_tag="VVN16_001203"
     CDS             13298..14332
                     /locus_tag="VVN16_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5572596.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            14334..16577
                     /locus_tag="VVN16_001204"
     CDS             14334..16577
                     /locus_tag="VVN16_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU5572597.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLYKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDRVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            16628..>16693
                     /locus_tag="VVN16_001205"
     CDS             16628..>16693
                     /locus_tag="VVN16_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5572598.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYK"
BASE COUNT         5426 a         2663 c         3419 g         5185 t
ORIGIN      
        1 ttctgaacta gcagagttat taaattgtac agaacgtgca gtcaaagatg atctatccca
       61 tgttaaatct gcttttcctg acttgatttt tcattcttct actaatggta tacgcattat
      121 taataccgat gatagtgata ttgaaatggt ttaccatcat ttctttaaac attcaactca
      181 tttttcgatt ttagaattca tcttctttaa tgaaggttgt caagctgaga gtatttgtaa
      241 agaattttat atcagttcat cttcgctcta tcgtattatt agccaaatca ataaagtgat
      301 taaaaggcaa tttcaatttg aagtcagtct gacccctgtt caaatcattg gaaatgagag
      361 agacattcgt tacttttttg cacaatattt ttcagaaaaa tattatttcc tagaatggcc
      421 atttgaaaat ttttcatcag agccactatc tcaattgtta gaattggttt ataaggaaac
      481 aagctttcca atgaatttgt caacgcatag aatgctaaag ttgctcctag ttacgaacct
      541 atatagaata aagtttggtc attttatgga agtagataaa gattctttta acgaccaaag
      601 tttggatttt ttaatgcagg cagaaggaat agaaggtgtt gctcagagtt ttgaatcaga
      661 atacaatatc tctttagatg aagaagttgt ttgccaatta tttgtgtctt actttcaaaa
      721 aatgtttttc atagatgaaa gtctctttat gaaatgcgta aaaaaggata gttatgttga
      781 aaaatcttac catctattga gtgattttat tgatcagatt tcagtcaagt atcagattga
      841 aatggagaat aaggataatc tgatttggca tctgcataat accgcacatc tgtatcgtca
      901 agagttgttt actgagttta ttttatttga tcaaaaaggg aatacaatca ggaactttca
      961 aaatattttt ccaaaatttg tttcagatat aaaaaaagag ctttctcact atttagagac
     1021 tcttgaggtt tgttctagtt caatgatggt aaatcatcta tcttatacct tcatcactca
     1081 taccaaacat ttggtgatta atttgctaca aaatcagcct aaactaaagg ttttagttat
     1141 gagtaatttt gatcagtatc atgcaaaatt cgttgcagag acactttctt attactgtag
     1201 caataatttt gaactggaag tttggactga attagaatta tcaaaggaat ctttagaaga
     1261 ttcatcttat gatatcatta tttctaattt tattattcct cctattgaaa ataagagact
     1321 catctattca aataatataa acacggtctc actcatatat ttgttaaatg ccatgatgtt
     1381 tattcgatta gatgagtaac caaaaataac aaaaaaacgc tatttcgcgt ttgaactgta
     1441 cctcaaaagt tagacagaaa aaatctaatt tttggggtgt ttttgttaag aaaagacctt
     1501 caatcattta agcaaaatgt acatctagat ttatagccct ctaaaactct tgcttttttt
     1561 gtagaagata caaaaaagcc ataaaaccaa ggcttttcct gttttatgta gatgtttaca
     1621 catgaaaatt gaagtatttt caaaagctaa ttggagtgat tttattcatg atgggataaa
     1681 tgatctggcc aaaaatgatt gagaaatagt tgcttaaaaa gcatttgcat gatactatat
     1741 attgaaagga ggtgataata tggtagaaca aagaaaatca attaccatga aagatgttgc
     1801 tttagaagca ggagttagtg ttggaactgt ttcacgtgta attaataaag aaaaaggcat
     1861 taaagaagta actttgaaaa aagtggaaca agcgattaaa actttgaatt acattccaga
     1921 ttactacgct agaggaatga aaaaaaatcg aacagaaacg attgcaatca ttgtaccaag
     1981 tatctggcat cccttctttt cagaatttgc tatgcatgtg gaaaatgaag tctataagag
     2041 aaataacaaa ttactcttat gttctatcaa tggtacaaat agagagcaag actatctgga
     2101 gatgttgcgt cataataaag ttgatggagt ggttgccatt acctatagtc caattgaaca
     2161 ttacttgacg tcaggaattc cctttgttag tattgaccgc acatactcag atattgccat
     2221 tccttgtgtt tcatcagata atgatgcagg tggaagagaa gcagctaaac aattaattag
     2281 taaaggatgt cagcatttag cctttgtggg cggacataat acaaccatta atgaaaccaa
     2341 acgtcgtaga atttcatttg aaaagtatgt ccaagagcat aaaatacctc ttagtatttt
     2401 tgatttggat gagacagtta ctgactatta tggaaagcta gagagtttct tggaagaaaa
     2461 ttcttctata gatggaattt ttacaatcaa cgattttaca gctttggatg tgattgaaat
     2521 actaaaaaaa aatggcaaac gtattcctca tgatgtccaa attattggat acgatggaat
     2581 taaaatggct ggagatagag attatctact ttcaactatt gaacaaccat tggaagaaat
     2641 ggcaaaagaa gcggttcgta ttttgtttga tataattgat ggaaagactg ttaatttgca
     2701 gacaatccta ccagtaaaat ttgttgaagg aaaaacaaca aaaaatgaaa ataagtcttg
     2761 acagaaagcg ctatcaatga tagaatgaat tcagataaaa agatttattt ttaaaacaaa
     2821 aatgaaacgt ttcaaaaaga gaaatgaaga gacagcgtca agcgctatct tttctagaaa
     2881 aaaatgaaac gtttcaaaaa ggaggttgct atgaatagca aagcgaagca agtttctctt
     2941 tgggaaagaa tcaagaaaca aaaactcttg ttattgatga ctgtccccgg tttagtttta
     3001 acctttatct ttaaatacat ccctatgtat ggggttttaa tcgcatttaa agattacaat
     3061 cctttaaaag gaattttagg gagtgattgg attggttttt ctgagtttac aaaattcata
     3121 tcctctccca actttggtat cttgttagcc aacacattaa aattaagtat ctatggttta
     3181 ttgcttggct ttttaccacc aatcattctt gctattatgc tcaatcaact cttgagtgaa
     3241 aaagtcaaaa aacgaattca gctcatttta tacgcaccaa actttatctc agtcgttgtt
     3301 attgtcggta tgattttcct cttcttttca gtgggaggac caatcaacaa ttttctttct
     3361 atgtttggaa tgaaggctga cttcttgaca aatccagact tctttagacc cttatacatc
     3421 tttagtggta tctggcaagg aatgggctgg gcttcaacgc tctacacggc aacattggta
     3481 aatgtagatc cagccttagt agaagcagcc cgactggatg gagccaatat cttccaacga
     3541 atctggcaca ttgatattcc agctcttaag cctattatgg ttatccaatt tgttttagct
     3601 gcaggtggaa ttatgaatgt cggatatgaa aaagcattct tgatgcagac atcgttaaat
     3661 ttgccaactt ctgaaattat ctcgacatat gtctataaag ttggtcttgt atcaggagac
     3721 tattcttact caacagcggt tggtttgttt aatgcagtga ttaacgtagt attgcttgtt
     3781 gcagttaacc aaatcgttaa acgcatgaat aatggtgaag gaatttaagg agggaagtat
     3841 gaaaaattcg attatggata caaaatttga tagacgtatc ttactcttaa ataaaatcat
     3901 tattgtcttt atcgttttga tgactttgct tcctttactt tatatcgtcg tagcatcctt
     3961 tatggatcct aaggttctgg ttagtagagg gattagcttt aatccagccg attggactgt
     4021 agaaggttat cagcgtgtat tcagtgacta atctattcta agaggtttta tcaattctct
     4081 actatactct tttggatttg cagctttaac agtattgcta tctgtgttta cagcttatcc
     4141 tctttctaag aaagacttgg ttggacgtcg ttggattaac tacttcttga ttgtgactat
     4201 gttctttggt ggtggtttag tcccaactta cttgctcgta aaagaattgg gaatgctcaa
     4261 tactccatgg gctatcattg ttccaggtgc tgtcaacgtt tggaatatta ttcttgctag
     4321 ggcctatttc caaggattgc ctgaagaatt agttgaagct gctgtcattg atggtgcaaa
     4381 tgatttacag attttcttca aaatcatgct tcctcttgca aaaccaatta tgtttgttct
     4441 cttcctttat gcttttgtag gacagtggaa ctcatacttt gatgcaatga tttatatcaa
     4501 ggatccaaac ttggaaccat tgcaacttgt acttcgtaaa attctcattc agagccaacc
     4561 aggtcaagac atgattggag cacaagcggc tatgaatgaa atgaaacgtt tagctgaatt
     4621 gattaaatac gcaactattg tcatttccag cttgccattg attgttatgt atccattctt
     4681 ccaaaaatac tttgataaag gaattatggc tggttcactt aaaggataaa aaaagaaaaa
     4741 ataaaaggag ttttctcatg aaattcaaaa cattctcaaa atcagcagtt ttgttgacag
     4801 ctagtttagc agtacttgca gcctgtggct caaaaaatac agcttcaagt ccagattata
     4861 agttggaagg tgtaacattc ccgcttcaag aaaagaaaac attgaagttt atgacagcca
     4921 gttcaccgtt atctcctaaa gacccaaatg aaaagttaat tttgcaacgt ttggagaagg
     4981 aaactggcgt tcatattgac tggaccaact accaatccga ctttgcagaa aaacgtaact
     5041 tggatatttc tagtggtgat ttaccagatg ctatccacaa cgacggagct tcagatgtgg
     5101 acttgatgaa ctgggctaaa aaaggtgtta ttattccagt tgaagatttg attgataaat
     5161 acatgccaaa tcttaagaaa attttggatg agaaaccaga gtacaaggcc ttgatgacag
     5221 cacctgatgg gcacatttac tcatttccat ggattgaaga gcttggagat ggtaaagagt
     5281 ctattcacag tgtcaacgat atggcttgga ttaacaaaga ttggcttaag aaacttagtc
     5341 ttgaaatgcc aaaaactact gatgatttga ttaaagtcct agaagctttc aaaaacgggg
     5401 atccaaatgg aaatggagag gctgatgaaa ttccatttac atttattagt ggtaacggaa
     5461 atgaagattt taaattccta tttgctgcat ttggtatagg ggataacgat gatcatttag
     5521 tagtaggaaa tgatggcaaa gttgacttca cagcagataa cgataactat aaagaaggtg
     5581 tcaaatttat ccgtcaattg caagaaaaag gcttgattga taaagaagct ttcgaacatg
     5641 attggaatag ttacattgct aaaggtcatg atcagaaatt tggtgtttac tttacatggg
     5701 ataagaataa tgttactgga agtaacgaaa gttatgatgt tttaccagta cttgctggac
     5761 caagtggtca aaaacacgta gctcgtacaa acggtatggg atttgcacgt gacaagatgg
     5821 ttattaccag tgtaaacaaa aacctagaat tgacagctaa atggattgat gcacaatacg
     5881 ctccactcca atctgtgcaa aataactggg gaacttacgg agatgacaaa caacaaaaca
     5941 tctttgaatt ggatcaagcg tcaaatagtc taaaacactt accactaaac ggaactgcac
     6001 cagcagaact tcgtcaaaag actgaagtag gaggaccact agctatccta gattcatact
     6061 atggtaaagt aacaaccatg cctgatgatg ccaaatggcg tttggatctt atcaaagaat
     6121 attatgttcc ttacatgagc aatgacaata actatccaag agtctttatg acacaggaag
     6181 atttggacaa aattgcccat atcgaagcag atatgaatga ctatatctac cgtaaacgtg
     6241 ctgaatggat tgtaaatggc aatattgata ctgagtggga tgattacaag aaagaacttg
     6301 aaaaatacgg actttctgat tacctcgcta ttaaacaaaa atactacgac caataccaag
     6361 caaacaaaaa ctagaggttg attatgggag ataagaaata cacagtagaa aaagccaatc
     6421 gttttatagc agaaaataaa catctcgtta atactcaata taagcctgaa gaacattttt
     6481 cagctgagat tggttggatc aatgatccaa atggatttgt ctattttcgt ggagaatacc
     6541 atctctttta tcaattctat ccatatgata gtgtttgggg gcctatgcac tggggacatg
     6601 ctaaaagtaa ggacttggtg acttgggagc acttgccagt ggcatttgct cctgaccaag
     6661 attatgaccg aaatggttgt ttctcaggct ctgccattgt caaggatgat cgcctctggc
     6721 tcatgtacac tgggcatatc gaagaaaaaa ccggtgtccg ccaagtgcaa aatatggcat
     6781 tttcagacga cgggattcac tttgaaaaga ttgaacaaaa tccagttgca actggagcag
     6841 acttgccaga tgagttgatt gctgctgatt tccgtgatcc aaaactcttt gaaaaagatg
     6901 gacgctatta ctccgtagta gctgccaaac acaaggataa tgtgggctgt atcgttctac
     6961 tagggtccga taacctagta gaatggcagt tcgaatccat ctttttaaaa ggggtagaac
     7021 accaagggtt tatgtgggaa tgcccagatt acttcgagtt agatgggaaa gattgcctta
     7081 ttatgtcacc catgcgttat cagcgtgagg gagactcata tcataacatc aactcatcgc
     7141 ttttgttcac gggtaaggta gattggggag aaaaatgttt tatcccagaa tcagttcaag
     7201 aaattgatca tggccaagac ttctatgcgc ctcaaacatt gttggacgat caaaatcgtc
     7261 gtatcctgat tgcttggatg cagacatggg ggcgtaccct tccaacccat gaccaagaac
     7321 acaaatgggc atgtgccatg actttaccta gaattctaag attggaagat ggcaaactaa
     7381 gacaattccc tgttaaaaaa ggccaatatc aaatccaaat agataaagat tgtcattacc
     7441 acttaggaaa tgatacagat tatcttgaat ttggttatga cagtaatgcg cagcaagttt
     7501 acattgatcg tagccatctt attcaaaaaa ttctaggtga agaagaacag gacactagtc
     7561 gacggtatgt agatattgaa gctaaagaat tggaagttgt tctagataaa aattccatcg
     7621 agatttttgt caatcaaggt gaagcaagct tgactgcaac ttattactta acggtgccag
     7681 ctgagctatc acgaattgat taaaaattaa gttatttctc ctaaagaaaa agttctcttt
     7741 ctaaaatagt ggaaagagga ctttttgtgt tttgggtata taagcttagt ttatggtatt
     7801 tgtaaaattg gtgttggatt atgatttaag ctagttttct aaagaatttg aaaaaaattt
     7861 tatttaagca aaaaaacctt ggttccaagg cttttcctgt tgtatttaga tgccccctac
     7921 agggatcgaa cctgtgaccc acggattaag agtccgctgc tctgccagct gagctaagga
     7981 ggcaaagaaa aaagctgtat tggtgccgaa acttcacggt ttgtattgaa cccgcgcaat
     8041 taagcaggtg ggcaactcgc tctaactgaa gctgtttccg tgtgagacgg cctacatgct
     8101 gttagaagac ttttgtttcc ctaataatac aaaaaatagt cggtcaacac ttaagtgtga
     8161 agtcgtacac cacagcgttt ctatacttat atgataccac tttttcaaaa aaaatcaaga
     8221 ggaaagtgca attttttgaa aaggatttca gatttttcgc aaacggtcga tagcttcctt
     8281 tctttgagcc aaagcccgtt catatttacc agtatcttct gcttggaaat agtgatgatt
     8341 acgaattttt tctggcagat agtcttgctt gacccaattt ccaggatagt tgtgtggata
     8401 gagatagtct tgggcattcc ccagttcctt gcttccactg tagtgcccat cacgtaggtg
     8461 tcgcggaata ggcaagtgcc ctgatgtttt gaggtcagca agtgccttat ccatagctac
     8521 ataggctgag ttggattttg gagaaagggc caaatcaatc acgacattgg caatgagaat
     8581 gcgggcttct gggaacccaa tcttctgggc agcatccaga gcagtcacgg tatgaatctg
     8641 ggcctcagga ttggctaagc cgatatcttc ataggcgata acagtcaagc gacgagcgag
     8701 actaggcaga tccccagcct caatcaagcg ggcagcatag tggagactgg catccacatc
     8761 tgagccacgg atagactttt gcagggcaga tagaacatca tagtgaccgt ctccatcctt
     8821 gtccatagtg atgtagcttc tctgaagact attttccatg atgtctaagg tgatgtggcg
     8881 aatgccctcg tcattctcag gggtagagag aacagccaag tccagtgagt taaaggcaga
     8941 gcgaaggtct ccgtttgtag aggttgcgat gaaatccagc gcatcctcat ctagttctat
     9001 tggaaaatta aaaccacgtt cagggttact tagagctatc tgcagggcct ctttgacgtc
     9061 ttggttagac agaggttcca actcaaaaat ttgaacacgg ctacgaatgg caggagtgac
     9121 ggaaaagaaa ggattttcag tcgttgctcc aatcatgatg accagtccac tttccaagag
     9181 aggcaagagg aagtcttgct tggtcttatc tagtcgatga atttcgtcta gcaataggac
     9241 gagaccacca gaaaatttag cttcttccga gatttcttgc agtcgctttt tactatcaac
     9301 tgtcgcattg aaagttcgaa aggcatactt ggtcgttcca gcgatggcag aggcaatact
     9361 ggttttgccg attccaggag ggccatatag aatcatggag gacaggcggt tggcttccac
     9421 catgcggcgg atgatttttc caggtccgac cagatgctcc tgaccgatga cctggtcgat
     9481 ggttttaggg cgcatgcgaa gcgcgagatt gtctggcata gcagtccttt ctaacgtaga
     9541 tttgagtaga atgactcatc tactatatct tactatgtcc attctagcct attagttgtg
     9601 atatttcaat ggttttgtaa taatcaggga gtgagataga atctttttta ctattcaatt
     9661 taatgaggag aggtatcagc atgagagatt tgattttcct taaagctcaa aataggatag
     9721 ttgagcctaa agaagtggaa gtgctgactt gagaaataat attgctaaat gtcattgtat
     9781 gaactataat gaaaagagag gtgataagca atggaaatga tggaactacc aagtcaggaa
     9841 attttgattt ttacaaaaca aattcgtcat tggattctta gtgatcaagt tatttcaggg
     9901 aaaagaaaac ttttcttccg ggaagatact ccaaaagaga ttttagattt gtacgaaaac
     9961 attaaatcta aacttgattt tgcttaccaa gaagttcatt ctaataatgg gttgaaaaaa
    10021 tatgaaaaat agaataattg atgtttttga agtagtaaat cgcctccttg taataactgt
    10081 tgaaaatcct gatttcgaag acttgagagt taatcatact caatgaaaat caaagagcaa
    10141 actaggaaac tagccgcagg ctgtacttga gtacggtaag gcgacgttga cgtggtttga
    10201 atttgatttt cgaagagtat cagtttgtaa aaattggaga taaaaaatat agagtacgca
    10261 gtgttcctat gattcattca actccgcctc aatctgtctt agatcgagat acttttacaa
    10321 ttgactatgc agatgatgaa ttgctcgata aagaagcagt ttttactact cattagaaaa
    10381 ataggtatga ataaattggc ttcccttaga accacatcta ggggctttta tgtataatag
    10441 aataataagt atcactcctt tacaatacgt gtaacacgtg ttataatggt gttgtcagga
    10501 ggtaaaagcg ctatgcctat gacacaaaaa gagatggtta aactccttac ggcccatggt
    10561 tggataaaga ctagaggcgg taaaggctcc catattaaaa tggaaaagca aggggaaaga
    10621 cttatcacaa tccttcatgg taagctgaat aagtacactg aaagagggat aagaaagcaa
    10681 gctgggttgt aaaattcagc ccctgctttt tgatatggag gtagtgaaat gttagttacg
    10741 tatccagctt tattttatta tgacgacaca gacggaacag aagcgactta ttttgtccat
    10801 ttcccagatt ttgaatactc agctacacaa ggagagggga tttctgaggc tttggctatg
    10861 gggtcggagt ggctagggat aactgttgca gatttgatag agagtgatgg agagttacct
    10921 cagccatcag atatcaatag cttgtcttta attgataatg atccttttaa agatgatgaa
    10981 gatttcgtgt caacctatga ccttgataaa tcttttattt ctatggtatc tgttgatgta
    11041 tcagaatacc taggaagtca ggaacccatt aaaaagactt tgaccatacc aaaatgggca
    11101 gataagttgg gacgagaaat gggacttaac ttttctcaga ctttgacaga cgctattgca
    11161 gataagaaag ttcaagccta agattaagcg tggtatcgtg gtaatagtca ataaaaaagc
    11221 acgtccactt gtgctagtta ctcgcctatt gattttatag atttattagc cctttcgcgg
    11281 gctttttata ttgattttta tgagaaatga agaaatcaga cttttaagaa aatcactgat
    11341 accaagggtt taaatgaggt aatatggtat aattaggaca taaaataatt ttgtggtaag
    11401 atggtagtat ctattttagc atatttccga gcaatcgggg cgattaaaga gtcgcataga
    11461 aagaggacaa aatggcaaca tacggatttt tagatgtttt agaggaagag ttggacaaga
    11521 actttccctt tgactttgag attagctggg acaaacgcaa ccatgcggta gaagtgagtt
    11581 ttctattgga agcgcaaaat gctgcaggtg tggagatggt ggatgaagac ggagaggttt
    11641 catcagatga cattcttttt gaggaagcgg tccttttcta caatcctgct aagtcaacag
    11701 tcaatgagga agactatttg actgttattc cttacctgcc taaaaaagga ttttcccgtg
    11761 aatttttagc ttattttgtg cttttcctta aagatactgc tgaggttggg ctagatgtcc
    11821 tcatggactt tttggaagac ccagaagcag aggaatttgt catggaatgg aaccaagaag
    11881 tctttgaaga aggaaaagtt ggcttggaag agggagaatt ttatccttat ccaagatact
    11941 aggagttggt tggaggtgtt atgaagaaaa ttgggactta tttggtttat gtgctagctg
    12001 ttgtctttat tatgctagct tttgcttgtg gaacaatcgc atttgcagag ttggggtatt
    12061 ccgcagtttt agcctttact tttggttatg ccttcgctct tctaagcatg tatttaatct
    12121 ttattcttca tgagctgggt catgcttttt gtggctactt gacaggctat cggctggtgg
    12181 cttttggatt aggacatttt attttgacca aaaagtcagg caggtttcat cttagtagaa
    12241 cagccattct gaaaaatgtt ggtgctcaat acattggttt aaaagaggat gaaagcgatc
    12301 aaagaatcat cctgatgctt tcaggaggct tgatggttca tctcggcttg atattattgg
    12361 cgatagtgtt tggatttttg acaagaagct ggtattttgc agggacttgg atttttctta
    12421 atttatcttt cttcctaaat aacattttgc cagtcgacat caccgatgga gcaaaaattt
    12481 gggaattact acaacaccct gaaaatacca aatacgccta cctggtattg agacattctg
    12541 ctcaaacttt gctggctcct caagaatatg atttgaaaga ctttgttcaa gcagttcctg
    12601 aggatgcacg agggagtttt gcggatggtg tattaggtat gcaaggtgaa gtttccattt
    12661 tggaagggaa ggaagaagta gctaaacagc agtttcaagc attacttgag aggacaacta
    12721 ctccgatgat gcagacagtt gcccaactat ccctgcttca tatcgccttg ttggaaggag
    12781 attttgagca agcggagcaa tatgctagta ttcgacaggt caaatcgttt ctgtctctca
    12841 aactgtccaa tcttcaagtg atacaagcct ggtatcaatt aaaggtgaag aaagatgtag
    12901 atcgaactcg taaggctctc aagattgcta gacagaaaat ggatagtagt cgaatgttga
    12961 gggacgagaa acgctattat gaaaacttgt tagcagagct ggaaaaggaa atgttagaag
    13021 gaactgaaga tggaactaga aatttctgat ttcctaggct gtaagattgc tttgttttgt
    13081 ggggataagc ttttgactat cttacgcgat gataaggcaa gcattccctg ggccaatatg
    13141 tgggaactgc caggtggggg aaagagtgtc agggaaaatc tgatatagtt ttgcattaaa
    13201 agaatatcct ttcaaggtta ttctttttta attttgtccc aaaataattg actttgtccc
    13261 gaaaatatag tattatttgt ataaggagga atgaatcatg aaaaaatcag atgttcttga
    13321 tttaataaag tatcattatg agggcagaga gacagaattt agaaatcaat ccatagcaat
    13381 tgctcggaac ttcaataaac atggtgatac acaaatagca caatatatta tggggttaat
    13441 gtctcaaagt gatagattca tgccacaaat agaaaatcct agtgagtatt tgactcctgc
    13501 taaactagat attggtcctc tcccattgcc tctttcaatt atgaatgatt taaaaggaat
    13561 aataaacgct gtcaatcatc atattggaat aaataaattt ttatttgtgg gatctccagg
    13621 tactggaaaa actgaaagtg taaagcaggt ggctagactt attggcaagg aattacttgt
    13681 agtagatttc agtcacttag tagatagtaa gctagggcaa acagtcaaaa acttagcaac
    13741 tctttttaat gaaatcaata atcttccttt caagcaaaat tatatcatcc tatttgatga
    13801 aattgattcc atagtgttgg atagggttaa tcagaatgat ttaagagaaa tgggacgagt
    13861 gacttctgcc tttttaaagg agttggatag gctgtcacca gaaattgtat taattgcaac
    13921 aacaaatctt tttgaaaatc ttgataaagc agttacgaga agattcgatg ccataattga
    13981 ctttgaccgt tatactgatg aagataaagt agaagtagct actattattt taaatgagtt
    14041 attaaagcaa tttaagaatg tagctagaga tttaaaatta tttaaaaaaa ttatcaatag
    14101 tgccaatgta ataccgaatc ctggtgattt gagaaactca ataagaacgt cattggcatt
    14161 tagtgaccca tcagatccgc atgattacca aaaacgcctg ctaagaagtt tgcataatgg
    14221 tagaaattta tctatttcta aattatcgaa gctaggtttt actgttagag aaattgagat
    14281 tttgacaggt atttctaaga gtagtgtatc acgagagtta agcgaggatt aaaatgaata
    14341 cattattgac attacgaggt aaaagtttta caccaaagtc agcaaagggg aggggaccga
    14401 taacgattcc cgcagattct attgttacgc taaaccattt aaatgaactt tatacatccc
    14461 ttgaacgagt aaaagaatat tggtctaata acacaatcat tgatggagta cttattagtg
    14521 tgtattataa tcgtgtccta gctaagagca atcgtatcga tggttttttt aatgtgggtg
    14581 gcggaaatgc aattccaaat gaaacgattg tgggtgcaaa atttagtaat gataagagta
    14641 ggcatattat tacacactac atttcaaagg aagtgttata taagacaatt gagcatacta
    14701 aaaaagttat aacaatattc aaagattatt ttgctagtgc tcccatgacg agtatggtgt
    14761 tctccgactc ttcaaaattt aatcaaattg attattctaa gtatggtctg tcgaaatcaa
    14821 aattcaaaca atacttaaga gacgcttgct ttgtcaaagg atttggtatt gaacaggcag
    14881 atatgtctac tatacgtaat tctattgtaa caatctacga tgtaaaaact aatctcttaa
    14941 cattacttaa acaacttgat attgatgttt cagaagctaa catcatggac cagacaactg
    15001 ttttacttga cgaaaaaaat attgagttac tactgctaaa agcaccttat ctagtatcaa
    15061 tgatagtaga agatttttct agcctgtcac cagagaattt ttataatgat gggaacactt
    15121 tttataataa tctccctttt ccaactaatg aacctattat tggtgtcatc gatacgttgt
    15181 ttgatgatag agtttatttc aatgagtggg tagagtatca tgatttaatt tcaccagata
    15241 ttagcaaagg aagtcaagac tataagcacg gtacagcagt ttcctcggta atagtagatg
    15301 gagctagttt gaatccaaaa ctagatgacg gatgtggaag atttagagtc agacattttg
    15361 ggatatcttt acagagtggt ttcaattcct tcacaattat caaacagatt agagaaattg
    15421 tgtctcaaaa tcctgatatt aaggtttgga acctttcgct tggatcaaat gatgaaatta
    15481 gggaaaactt tatttctgtc gagggttcgt tgttagacga gattcaattt gataacgatg
    15541 taatttttat tattgcaggc actaatggga ttgcccctga tggacaacga aaaaagattg
    15601 gagcaccagc agattcactc aattcaatta ttgtaaattc tgttgatttt agcaatcaag
    15661 tcgtttctta ttcacgcgaa ggagttgtcc tgtctttctt tatcaaacct gatgtttcat
    15721 actatggcgg ggggaacgga gacttcataa atgtttgtga accactaggg ctggctcgag
    15781 tagctgggac cagttttgcg gctccattta ttgctagaaa aatggcttac ctcattcata
    15841 ttatgggatt aagtcgtgaa gaagcaaagg ctttattaat tgattcagcg ataccatgga
    15901 atgataaaaa aacattcgca gacatttctt tacttggata tggggttgtg ccaatcaaaa
    15961 tagaagatat tttgtctaca cctgatgatg aaattaaatt tatagtctca gatgtttcta
    16021 gagcatatga aacttataat tatgatttcc cagttccaat ttctaaagaa cactatccat
    16081 atgtagcaaa ggccactatg tgctattttc caaagtgttc tagaaatcaa ggagttgact
    16141 atactaatac ggaaatgcaa cttagtttgg gagttttaga taagaaaggc attaaaacaa
    16201 ttaataacga caatcaatat tctgaaaatg caccaggata tgtgagagaa aagtcggcac
    16261 gaaatatttt taggaaatgg gataatgtca aacatttagg agaaaaattt tcttctcgta
    16321 aaaaagctaa gccgattctg aatccttcta atcctcaatg gggaatgagt gtaaaaacaa
    16381 ttgaacggtt aagaaatgat gatggttatg gtattcgttt tggagtagtt gttactctaa
    16441 aggagttgaa tggtgtcaat agaatcgaag attttattca acaagccgaa ttacgcggtt
    16501 ggctagttaa tagattgcaa gtagaagctc aagtagacct ctttaatagt ttaaatgaag
    16561 aaattgaatt tgaatagtgg atattttatc cttattctag atcttaggaa cacaagaggg
    16621 caaagatatg aatattactt atatagttgg aaatggtttg gatttgcaat atggtttgaa
    16681 aacaagatat aaa
//
LOCUS       DAVHIT010000042        16384 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVHIT010000042 DAVHIT010000000
VERSION     DAVHIT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.043"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            150..1679
                     /gene="rlrA"
                     /locus_tag="VVN16_001206"
                     /pseudo
     CDS             150..1679
                     /gene="rlrA"
                     /locus_tag="VVN16_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
     gene            1944..3206
                     /locus_tag="VVN16_001207"
     CDS             1944..3206
                     /locus_tag="VVN16_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5572599.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTIVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFIRYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(3264..5588)
                     /gene="pflB"
                     /locus_tag="VVN16_001208"
     CDS             complement(3264..5588)
                     /gene="pflB"
                     /locus_tag="VVN16_001208"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU5572600.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5936..6997
                     /gene="dinB"
                     /locus_tag="VVN16_001209"
     CDS             5936..6997
                     /gene="dinB"
                     /locus_tag="VVN16_001209"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5572601.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(7144..7989)
                     /locus_tag="VVN16_001210"
     CDS             complement(7144..7989)
                     /locus_tag="VVN16_001210"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU5572602.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            8245..9113
                     /locus_tag="VVN16_001211"
                     /pseudo
     CDS             8245..9113
                     /locus_tag="VVN16_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9414..11303)
                     /locus_tag="VVN16_001212"
     CDS             complement(9414..11303)
                     /locus_tag="VVN16_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU5572603.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            11441..13006
                     /locus_tag="VVN16_001213"
     CDS             11441..13006
                     /locus_tag="VVN16_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU5572604.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYVSAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            13006..13746
                     /locus_tag="VVN16_001214"
     CDS             13006..13746
                     /locus_tag="VVN16_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5572605.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(13855..14070)
                     /locus_tag="VVN16_001215"
     CDS             complement(13855..14070)
                     /locus_tag="VVN16_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572606.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(14941..16191)
                     /gene="ilvA"
                     /locus_tag="VVN16_001216"
     CDS             complement(14941..16191)
                     /gene="ilvA"
                     /locus_tag="VVN16_001216"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU5572607.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(16241..>16384)
                     /locus_tag="VVN16_001217"
     CDS             complement(16241..>16384)
                     /locus_tag="VVN16_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5572608.1"
                     /translation="HRNDSEESLVEWLYDWISTNRYELPKEFSIRMAHKYHESVTEIF
                     GDE"
BASE COUNT         5151 a         3487 c         2935 g         4811 t
ORIGIN      
        1 caatactcat tactatcttt aaaatgcctt ttattacatg ctgttttatc aataaagtcg
       61 gaaattccac aacctttttg acaagacaat tagtttcttt tgtattataa taattaaaat
      121 tatctatatg caactaagat tcggtaacta tgctaaacaa atacattgaa aaaagaatta
      181 cagataaaat taccatttta aatatattat tggatattcg gtctattgaa ctagatgaac
      241 tgtctacttt aacttcatta caatctaaaa gtctcctaag cattttgcag gaattacaag
      301 aaacattcga ggaagagttg acatttaatt tagatacgca acaagttcaa ttaatcgaac
      361 accattctca tcaaacgaat tattacttcc atcagcttta taaccaatca actattttaa
      421 aaatactccg cttctttcta ctacaaggta atcaaagctt taacgaattt acacaaaaag
      481 agtatatttc catcgcaaca ggctaccgcg tacgacaaaa gtgtggcttg ttactacgtt
      541 cagtaggttt ggaccttgtg aaaaatcaag tagttggtcc tgaatatcgc attcgttttt
      601 taattgcact cctccagttt cattttggta ttgagattta tgatttgaat gatggaagta
      661 tggattgggt cacacatatg attgtgcagt ctaattctca actcagtcat gaactgttag
      721 aaatcacacc tgacgagtat gttcatttta gcatactagt ggcactcact tggaaacgtc
      781 gggaatttcc acttgagttc cctgagtcca aatagtttga aaaattaaaa aacctcttta
      841 tgtatccgat tttgatggaa cattgccaaa cttacttaga gccacatgcc aacatgactt
      901 ttactcagga agaactggat tatatctttt tggtttattg ttctgctaat tcttcgtttt
      961 ccaaggacaa atggaatcaa gaaaaaaaga ctcatactat tcaactgatt cttcagcata
     1021 caagaggaaa gcatctacta tccaagttca agaatatctt aggaaatgac atttcgaatt
     1081 ctctttcttt tttaacagca ctgactttct taacaagaac gtttttgttt ggtttacaaa
     1141 atctagttcc ctattataat tattacgaac attatgggat tgaaagcgac aagccactgt
     1201 atcatatttc gaaagcaatt gttcaagagt ggatgactga gcagaagatt gaaggagtta
     1261 tcgaccagca tcggctgtac cttttctcac tctacttgac agaaaccatc ttttcctcac
     1321 tacctgctat cccgattttt atcattttaa ataatcaagc agatgtaaac ttaataaagt
     1381 ccattatttt acggaatttt actgacaaag ttgcctctgt tacaggatac aatattctaa
     1441 ttagtcctcc tccttcagag gagcatttga cagagccatt gattattatt acaacgaaag
     1501 aatacctgcc ttacgttaaa aagcagtatc ccaaagggaa acatcacttt ttgacgattg
     1561 ccttagattt gcatgttagc caacagcgtc tcatctatca gacaattgta gatatccgaa
     1621 aagaggcttt tgacaaacgg gtcgcaatga ttgctaaaaa ggctcactat ttgttataag
     1681 ttcatcgtac tgtctacaca caaaaagggg cttcctaagc catctctatt cttatcccca
     1741 tatttcccag ttttgggtaa atctgaacag aaaaacactt ctgctctcta gtttctgcta
     1801 gtgaacaaaa gtgttttttc tggctctata atttctgtag tgggtaaatc ccactgtgga
     1861 gattataggg ctttttgagt atcaaaaaag tcccataaga tttataatga aaagcgccta
     1921 aacaactcat tagaaagtat cttatggaac acatcaatca taccacatta ctcatcggaa
     1981 taaaagacaa aaacatcacc ttaaataaag ccattcagca cgatacccat atcgaagtct
     2041 tcgctacact tgattatcac ccacctaaat gtaaacactg taaaggaaaa caaatcaaat
     2101 acgacttcca aaagccttct aaaatccctt ttatcgagat tggtggtttc cctagcctca
     2161 ttcatttgaa aaagagacga tttcaatgca agtcctgtcg gaaagtcact gtagctgaaa
     2221 caactctcgt tcagaaaaat tgccaaatct cggaaatggt gagacagaaa attgcccaac
     2281 tcctactcaa cagagaggct cttacacata tcgcttctaa attagccatc tctacctcta
     2341 cctctaccgt ctatcgtaag ctcaagcaat ttcatttcca agaggattac accactttgc
     2401 ctgaaatcct ctcctgggat gaattctcct accagaaggg gaaattggct ttcattgctc
     2461 aggatttcaa cactaagaaa atcatgacca ttcttgataa cagacgtcaa acaaccatcc
     2521 gaaatcattt cttcaagtac tcgaaagaag ctagaaaaaa agttaaagtc gtcactgttg
     2581 atatgtctgg aagttatatc cctctcatta agaaattatt tcccaatgct aaaattgttc
     2641 tcgatcgttt ccacattgtc caacacatga gcagggctct taatcagact agaatcaaca
     2701 tcatgaagca atttgatgat aaatctctgg aatacagagc tcttaaatat tactggaaat
     2761 ttatcctaaa agatagtcgg aaactctctc ttaagccttt ctatgctaga actttcagag
     2821 agaccttaac tcctagggag tgtctgaaga aaatctttac tatagtacct gaacttaaag
     2881 attactatga cctgtatcaa ctactcctat ttcatctaca agagaagaat actgaccagt
     2941 tttggggctt aattcaagac acgttacctc atctcaaccg cacctttaaa accactttga
     3001 gcacatttat tcgctataaa aactacatca ctaacgccat tgaattgcct tattctaacg
     3061 ctaaacttga ggccactaac aaactcatca aagacatcaa gcgcaatgcc tttggttttc
     3121 ggaactttga aaacttcaaa aaacggattt tcatcgctct gaacatcaaa aaagaaagga
     3181 caaaatttgt cctttctcga gcttagcttt tcttcaaccc actacagttg acaaagagcc
     3241 ctttttatta ttcaagaact tgattagctc aatgcatcca aggcgtcatc cattgaaaga
     3301 acttcgtgga agacacgttg tgtcaattca gttttttgtt ctggagtgag gtatttagtg
     3361 tttacacagt atccagagat acgtacgata acgtcttcgc ctgacatgat tttttcgtaa
     3421 acatcgttca agtccataac gttcaagtta acgtgttgtc caccgttttc gaagtaacca
     3481 tcaaggattg ttaccaagtt atcaacttgt tcatcacgag tcttaccaag agcgcgaggt
     3541 gatacttgtg tagtcaatga gataccgtca gctgcataac taaagtcaag gctagaaagt
     3601 gagttcaagt tttgcaacca accaccttta gctttgttag atgggttagc acctggtgag
     3661 aagaattcaa gtttagacaa gttcacagaa ccatcttcgt tgaggtatac acctttgtga
     3721 actggtgagt taccagtttg tttagagtaa gcaacgttag atgtgattgt caaaagtgat
     3781 actgtagctt ctgcgtcttt gtatagtttg tggctacgta gacgagttgt gtaagcttcg
     3841 atcaaccatt ctgccaattc gtttgaacgt gggtcatctt caccccagcg tgggtagtca
     3901 ccgattgttt cgtaatcgta gatgtagcca tcttcatcac ggattggttt aactgtagcg
     3961 tatttaatcg ctgacaatgt atcaacagtg ttagcaaatc cacaaatacc gaatcccatg
     4021 ttggcacgtt gtttagttgg caagaaggcc atttgaacag cttcgtagtt gtacttatca
     4081 gtcatgtagt ggatgatgtt caaggcatct acgtaagtgt cagtcaacca gtcaagagat
     4141 ttttcaaagt tcgctttaac tgattcaaat tcaagaactt cgtcacgaat aggatcgatg
     4201 tcaaatactt tgtagtcttt gtgaacatcg tcgtaaccac cattcaaacc agtaagaagg
     4261 gctttaagaa cgtttacacg agcaccgaag tactggatgt tgtggcgttg ttcttcattt
     4321 tctggatcaa gtggagacac acagcatgag atacagctca tttcaccata tccgtcttta
     4381 gccattgttg ttacaccttc gtattggata gaagagtgtt tgtggctcat gtgcatacag
     4441 tagcgacgga agttgtatgg caatttgtca gtccaaagaa ctgtcaagtt tggttctggt
     4501 gagttaccga tgttgtcaag agtgttcaag aaacggtagt ccatcttagt aacacggtga
     4561 cgaccgtcgt tacccatacc agccatagaa gttgtgataa aggttgggtc acctgagtac
     4621 aattggtcat aagctttggt acgagcaaat ttaactgtac gaagtttcat aacgaaatca
     4681 tcaacgaatt cttggatttc tgattcagta aatgtaccac gagcaaggtc acgttctgca
     4741 aagatgtcca atacgattgg tacacgacct agagatgtag cagcaccgtt aatcacacgg
     4801 cagacagcca tgaaagcaat gttaacccat tggattgctt ctttcacgtt catcgctggt
     4861 ttgcgaacat caaccccgta aaggtcaccc aggcgaacaa cttgttgcaa tgcttggtat
     4921 tgaaggttta cttcttcacg aagacggatt gtttcttcat cgatttcttt gattgcattc
     4981 cagtcgttta ctttttcttg catcaagtag tctgcaccgt aaagagcaag acgtgcgtaa
     5041 acaccgatga tacgtccgcg tgagtatgca tctggaagac cagttacagt gtgtgcatga
     5101 cgagcgcgac gaatatttga agtgtaggca cggaaaatac cgtcgttaac tgttgttaca
     5161 tatttagtga agatttcgtg aacagctggg tctggttcgt atccattttc ttttaaagta
     5221 gtttcagcca tacggatacc accttttggc atgaagttca atttgaagag ttcatcattt
     5281 tggataccaa agataacttc attttctttg tcgataaatc cagcagggat atcagcgata
     5341 gatgttggac gagtgtccat tgggaaacga gtttcttcgt agtgtgcttt agtttcttct
     5401 acaattttct tgatgtgaag tgaacgctct gttggtcctg caaggaagct ttcgtctcca
     5461 tcataaggtg tgtagttagc ttgtacaaag cgtgatacac ttgctttttc tttccaatct
     5521 acgcctttga agccttccca agctttgtca aaaatatctt gtgcttcaac aactgtctta
     5581 acaaccatat taatgtcctc ttttttctag taacagtcat ctgttacatt catgagacaa
     5641 gtataccata tagtaaccga tttcaacaag tgaaaaatcc ctatttttac actttctttt
     5701 ctaaaacagt ctatatttta tttcaaactg tattatattt ttgaaaaaat aaagtccttt
     5761 tttctttttt tcagaaaaaa gggtataata aaagaaaata agcagtaaca ctcaatggaa
     5821 atcgaaaagc aaactaggaa gctagccgca gattgctcaa aacactgttt tgaggttgca
     5881 gatagagctg acgtggtttg aagagatttt cgaagagtat aaaaaggtgc taggcatgtt
     5941 gatttttcct ttgttaaatg atttgtcaag aaaaatcatc catattgaca tggatgcctt
     6001 ttttgctgcg gtggaaatca aggataatcc taaactcaga ggaaaacctg tcattattgg
     6061 aagcgaccct cggcaaacag gtggacgggg agtcgtttct acctgtagct atgaggctcg
     6121 agcttttggt gtccattctg ccatgagttc caaggaagct tatgaacgtt gtccccaggc
     6181 cgtctttatc tcaggaaatt atgagaaata caaagctgtg ggactccaga ttcgagctat
     6241 ttttaagcgc tatacagatt tgattgaacc catgagcatt gacgaagcct atttggatgt
     6301 gacagaaaat aaactcggta tcaagtcagc ggtcaaaatt gctcgcctca ttcaaaaaga
     6361 tatctggcaa gaactccatc taactgcttc tgccggtatt tcctacaaca agttcttagc
     6421 taaaatggcg agtgattatc aaaaacctca tggtttgaca gtgattctac ctgaacaggc
     6481 tgagaatttt ctcaaacaaa tggatatttc caaatttcat ggagtaggaa aaaagacagt
     6541 agaacgtctt catcaaatgg gcgtttttac tggtgctgat ttacttgaag ttcctgaggt
     6601 gaccctaata gaccgttttg gtagactagg ctatgatctg tatcgaaagg ctcgtggcat
     6661 tcacaactct ccagtcaaat ccaatcgcat ccgtaaatca atcggcaagg agaaaaccta
     6721 cgggaagatt ctccgtgctg aggaagatat caaaaaagag ctgactattc tatcagaaaa
     6781 agtcgctctc aatctacatc aacaagaaaa agctggaaaa attgtcattt tgaaaatccg
     6841 ctacgaggac ttttcaactc ttaccaaacg aaaaagtctt gctcaaaaaa cacaagatgc
     6901 tagtcagata agccaaatag ccctgcaact ctatgaagaa ttaagcgaga aagaaagagg
     6961 tgtccgccta ttggggatta ccctgactgg attttaaagc tcaatgaaaa tcaaagagca
     7021 aactaggaag ctagtcgcag gctgctcaaa acactgtttt gaggttgcag ataaagctga
     7081 cgcggtttga agaaattttc taagagtata aaaccttgaa gagttgcccc ttcaaggttt
     7141 ttcttataca aataaacgga caaaactgta aagtagcaaa acactaccaa gcacgatacg
     7201 gtatttacca aaaagggtga agtcgtgttt tttcacatag ctggtcaaga agcgaatagc
     7261 caccatgctg accgcaaaag ctactcccat cgcgaccaag agcaaaaaca attgcccaaa
     7321 gctcaagagt tctccggctt tcacaaattt gaaaatcttt aaggcactag ctccaaacat
     7381 aacgggaatc ccaagataga aggtaaattc tgtcacaact gaacgactgg ttccatttaa
     7441 caaaccaccg acaatcgttg cacctgaacg gctagtccct ggtaaaagag caagaacttg
     7501 gaagagtccg atatagaaag cggtcgtata aggaagcttg tccaactctg ttacacttgg
     7561 ctcgatagca cgcgctttat tgcgcttttc caaatagatg aaggcaaccc cgtagataat
     7621 caacatgaga gcaactgaaa ccatgttatg gaagtgggta tcaaaccaat catcaaattt
     7681 aaagacacca agtaaaggca aagtggcaat caagaccttc aaccatagtc tccaagtctt
     7741 acgaacttcc tgtttgtcct tagtcggttt aaaaggattg agcttgttaa aataaatcac
     7801 cataactgct aaaatagcac caagctgaat cacgacatta aacatggaca taaaggcttc
     7861 attttgattt tggtattgga taaactcctc tgctaaaatc aagtgacctg tactggaaat
     7921 cggcaaccat tccgtaattc cttcaacaat cccgaagaag atagatttta aaatttcaat
     7981 aagatacata tatttctcct tatattatat ttaacgcagt tagctactgc caaagcccaa
     8041 gtggtatact tggaataagc cactgtggat tagttcattt cctccaatcg cttgacgatt
     8101 tccgaaaggg tctaaccaca gctttttgtt tcaattggta atgagtttga taacgcatag
     8161 acgttttatc tcgtctaagg taacaaggca aaaagttcgt ggataaccaa gtagacttga
     8221 aatactatat ctggaggtac tcccatgctt tatgtgggca ttgatgtcgc taaaaataaa
     8281 cacgatgtta cagccttgaa tgttcgagga aaactgttct taaaccactc actttttcaa
     8341 ataataaagc tggttttgaa ctcttagatc tgtcttttcg acagctcaac caagactgtc
     8401 tcatcgctct taaacttctt tctgacccca atcgtgaaca atttcaacac gataatcggc
     8461 aagtagaact aaaaatactg gctagacata ttcatcgtct caagaaaaaa cagtctgatt
     8521 ggaaagtaca atacactcgt tgtcttgata tcatctttcc tgagttggat aaaatcgttg
     8581 gaaagcattc agaatatacc taccaactct tgacacgcta ccctaatcct cagaaaaggc
     8641 ttgaggcagg atttgataag ctgatagaaa ttaagcgatt gaccgcttct aaaattcagg
     8701 atatcctctc agtcgcacct cgttctatcg gaacaacatc tcctgctcgt gaattcgaaa
     8761 tcatcgaaat catcaaacat tacaagaggc tcattgacaa ggcggaaaca tgtgtcaatg
     8821 acttgatggc tgagttcaac tcggtcatca cgacggttac tgggattggg ggtcgtttag
     8881 gggcggtcat tttagccgag attcgaaata ttcatgcctt tgataatcct gctcaattac
     8941 aagctttcgc tggactggat tcttctattt atcagtcagg tcagattgat ttagctggaa
     9001 gaatgatcaa acggggttcc cctcatctgc ggtgggcact catacaagct gccaaagcat
     9061 gcgctcgctt ttcacctgct tttaaggcct atcttaagac taagttagaa taaggaaaac
     9121 attacaatgt agccatcatc caccttgcaa aaaaacttat ccgaaccctg ttttatatcc
     9181 taaaaaagag ctgccatttg acgaacaaaa agtgatgtaa tttctgaaat taacacagga
     9241 ttacctttga aaaaatggac tttttcaagg gctttttgcc gtgctctaaa atcatcgcca
     9301 aatcatcaaa ttgaaataag aaatagttta gataaaacta ttgacttttc ttatagtttg
     9361 tctttcttct atcttccatt atatcatact ttttcagtct atggtagctc tctttaaaag
     9421 gctccgatac tgccgccacc tccgcctcca gagaagccac caccagaact tccacttcca
     9481 gaagatacag agtaggtact tgctgtattt gcgacactag cataatggct catttgcgcg
     9541 cttgaatgat aaaacatact gtgccagcca taagctacat agagattgat atctggattt
     9601 tcaacttgaa tctgatgaac cttcatcaaa tgactaacct tgtccgcata gccaaataag
     9661 gttgcataga ccaagagacg attccaaaca acaatacttt ccaattcagc cttatccagt
     9721 cgtgcaatct cacgtaacat attttcaaaa ctggtccaga gatagtagac ctcagctccc
     9781 gcttcattta gaacaccatc acgattatct agtcgaagct tccaataata gaaaacagac
     9841 aaaactaacc ctagaaaacc aagtattggc aaagggaggt aaagatagcc atgaacgtct
     9901 aaactgtaca agaacaaacc aaatccgata aataggggca agatagtcaa gacacccata
     9961 cccacttgca aagccttttc caaaccagtt aaaggacgat agtaatctgg gagtccccag
    10021 aagacaactc gctttctcac tccttcttgc atctctttta atactgcttc gaaagaagat
    10081 ttgagctgaa tccctcttgc ttgaatccgt ttctcatcag cagctttagc tccgcgataa
    10141 aggctgtcag ataccttgta atccgcaaac aaattggaaa gagtggcttc ttttttgcct
    10201 gaaaaggcta gatttagaca gtctctctca aatcttgaca aaccatcttc tttcaccaat
    10261 ctcaagccaa ctgcatctcc ttctgaaata atagaaacat tcccacggtc catcacatct
    10321 agcaaggtag cttgaataag ttggtcaaaa gtgaatttgc cagccccctt gactaaggga
    10381 ctcacttcct ccaaggaggt cgagtagaca gcctctgata aaaccatagg ctctaattcc
    10441 attggtggtt catagagacg atgatttttg gcatatttga ctgaaggagt ggtctttctt
    10501 ctataaataa aatagaagca gacactcaat aacaaggaga tagaaaggat cgaagggagg
    10561 acccaagtaa caagttgttt actctgatct ttttctctaa caatcgagtc ttctatctta
    10621 ttaaactctt ctaaacgatt ccctttcaat ccctgatccc tagcgctagc aaaatcggtc
    10681 cgaggccagt aggcatgcaa ctcaactcca cgcttagccg gaagattgtc taaacggata
    10741 gtataatcaa ggttactctt ttcaatcgtt ccctctctaa aaagtttccc tgtatggaaa
    10801 aagagttttt cagccccctt gtctcccctt acatgaaatt caaacttttc aatagactct
    10861 gaactatctg tcagaggttg ccaatttaat tcagcgatat catcataaag gaaaagtaaa
    10921 ttttttaagt tccagacgag gtcaacttca actatgtcgc cctcctgacc tggattatag
    10981 actctcacag tataaccatc cgcttcttct gttacttcgc tagtcacatc tgctagttct
    11041 gcaccgtttt tcgcggcctg aacctttgga tgagggtcaa tgtcaaaccc gctaggcatc
    11101 ttaccagcac gtccaagtcc cacgatttgt cccttaaagt cctcctcaaa ctggtaaact
    11161 atcttctgtc taaactctgc cgtattatct gcatgaatat acaaatcccc ttgataagag
    11221 tttatcttga aatcaatggc aaaaacagag agtggaagaa ggcaaaacaa gcctaacacc
    11281 agtaagaaaa aagttttttt catcaactaa gccccctttt tatctttctt atcattatag
    11341 cattttttcg taagtaaagg cttacttatg ttgaaaaatt acgcattttt cgataaaata
    11401 ggagacagtt attttttaat agatcagaat aggagaaatc atgagaaaaa tatacttatc
    11461 tattttcaca agtctcttgc tgatgctagg acttgtcaat gttgctcaag ccgatgaata
    11521 tttacgcatc gggatggaag cagcatatgc tccctttaac tggacccagg atgatgatag
    11581 caacggagct gtcaaaatcg atgggaccaa tcagtatgcc aacggatacg atgttcaaat
    11641 cgccaagaaa atcgctaagg acttaggtaa agaacctttg gttgttaaaa ccaagtggga
    11701 aggtctagtc cctgccctta cttctggtaa gattgacatg attatcgcag gtatgagtcc
    11761 aactgcagaa cgcaaacaag aaattgcctt ttcgagcagt tactacacta gcgaaccagt
    11821 tttgcttgtc aaaaaagatt ctgcctacgt aagtgctaaa tctttggatg actttaacgg
    11881 tgcaaaaatc acttctcaac aaggggtcta cctctacgac ttgattgcac aaatcccagg
    11941 tgctaaaaaa gaaacagcca tgggagactt cgctcaaatg cgccaagctc ttgaggctgg
    12001 tgtcattgat gcttatgttt ctgaacgtcc agaagcactg actgctgaag ctgcgaactc
    12061 taagttcaag atgattcaag tagaacctgg tttcaaaact ggggaagaag atacagctat
    12121 cgctatcggg cttcgtaaaa atgacaatcg tattagccaa atcaatgcca gcattgaaac
    12181 catttcaaaa gatgaccaag ttgccttgat ggatcgtatg atcaaggaac aacctgccga
    12241 agctacaaca actgaagaga ctagcagtag tttctttagc caagtcgcta aaattctttc
    12301 tgaaaactgg caacaactct tgcgtggtgc tggtatcact cttttaatct ctatcgtcgg
    12361 aaccatcata ggtctcatta ttggacttgc cattggtgtc ttccgtactg ctcctctctc
    12421 tgaaaacaaa gccatttatg gccttcaaaa actagtcggc tgggttctca atgtctacat
    12481 tgaaattttc cgtggtacgc caatgattgt tcaatcgatg gttatctact atggaactgc
    12541 ccaagctttc gggatcaacc ttgaccgtac actagctgct atcttcatcg tttcaatcaa
    12601 taccggtgcc tacatgactg aaatcgtccg tggtggtatc ctagcagttg acaagggaca
    12661 atttgaagct gcgactgctc ttggtatgac ccataaccag accatgcgta agattgtcct
    12721 acctcaggta gtccgtaaca tcctacctgc aactggtaat gaatttgtca tcaatatcaa
    12781 agatacatct gtattgaacg ttatctctgt tgtcgaactt tatttctcag gaaataccgt
    12841 ggcaacacaa acctatcaat acttccagac atttacaatc atcgccgtga tttactttgt
    12901 cctcaccttc accgtaacac gtatcctacg ctttatcgag cgcagaatgg acatggatac
    12961 ctacactaca ggtgctaacc aaatgcaaac ggaggatttg aaataatgac acaagcaatc
    13021 cttgaaatta aacacctcaa aaaatcctat ggacaaaacg aagtgctaaa agacatttca
    13081 ctcactgtcc acaagggaga ggtcatctct atcatcggaa gctctggaag cggaaaatcg
    13141 accttcctac gctccattaa cctacttgaa acaccaactg atggacaaat cctttatcat
    13201 ggacaaaacg tcctcgaaaa aggctatgac ctcacgcaat accgtgaaaa gttggggatg
    13261 gttttccaat cctttaacct ctttgaaaat ctcaatgttc ttgaaaacac aatcgtcgct
    13321 cagacaactg tcctaaaacg cgaacgcaca gaagctgaaa agattgccaa agaaaacctg
    13381 gaaaaggtcg gcatgggaga acgctactgg caagccaaac caaaacaact ctcaggtggt
    13441 caaaaacaac gtgtggccat cgctcgtgcc ctctccatga atccggacgc cattctcttt
    13501 gatgaaccaa catcagctct cgatccagaa atggttggag aagtcctcaa aatcatgcag
    13561 gacctggctc aggaaggctt gaccatgatt gtcgtaaccc atgaaatgga atttgcccgt
    13621 gatgtctctc accgtgttat ctttatggat aagggcgtga tcgctgaaga aggtaaacca
    13681 gaagacctct tcaccaatcc taaagaagac cgaacaaaag agttccttca acgctatctc
    13741 aaataaaaag aaaaggctgc atcaaccgtg cagtcttttt gctgtcctca aaatgaaaag
    13801 gcagattctc tatctctact acttgaaggt caagaatccg ccgttatcca aagattaatc
    13861 ttcaaatttt tcataatttt tttcatagaa atcaattagg cctagggctg tttcaaatga
    13921 aatatttttt acttttgcac gaccctgcgc cagagcaatg atagacattt cacgcgcatt
    13981 cgtttcttta gagatacggt agcctgtgat tttcttatca cgaacccagc caacaactga
    14041 ttctactttt tcaaagtttg acttagccat gtttttctcc tattttcttc tttataatat
    14101 ttaattgtat acgtttttac tacttttgtc aataaaaaaa acatatattc aacgaaaata
    14161 attagttcag aattttctta cttccttttt aaatccgata atatataggt ttcgctagcc
    14221 tctacaccat aaatatttgc taataaaaat actgtattat taccctctta aggtgcgaaa
    14281 ttattcaagt tcttaaaaaa tgcttctcta tttccgtaag atagcttatt tcttcgattt
    14341 aagaactgaa gatgatagtt ctaaccttta gaaatccaag ccttcactag ctcaaaaact
    14401 cctatctttc ttcctcaata aatcatctga gagataggta ttccttatgt ccttcaaatt
    14461 catcactcct ttggatattg aggtgctagc ctagaagcag acacccgatt atctaaacca
    14521 tccttaataa agatgacaag acagataagc tcagttaatt acttatatat ctttttaata
    14581 tggtatattt gtcaacaaaa agaaatcaaa tcatacctta tactcaatga aaatcaaaga
    14641 gcaaactagg aagctagccg caagttgctc aaaacactgt tttgaggttg cagatagagc
    14701 taacgtggtt tgaagagatt ttcgaagagt attaaagaca gtgtgactat gcttgtcaat
    14761 agataggaaa tcaaaatgaa ggatggaaat ctgagccaaa catcataagt gctataatga
    14821 acagaagtaa gaattgcacg ataagttttc taaaaccata ggaatcgtcc cctttcttca
    14881 tcttcattat agcacatata catcagttgt acaaataata tgatattttt ttattagtcc
    14941 tcagacaagc atattataaa gcgtttcatt accatttaag ttaatataag ctggatcaaa
    15001 accttccatt ctacgaatca aacctgcata atcatgctta tctgctaaag cgatcccaat
    15061 taatactggg cctgttccct tgctagctcg tttgatatac tcaaaacgtg tgatatcatc
    15121 atttggcccc aggatatcat ttacaaactc acgcaaagct cctggacgtt gtgggaaatt
    15181 gaccacaaag taatgtttga taccatcata aatcaaggca cgctcttcca tttctggcat
    15241 acggttgata tcattatttc ctccagaaat gatacaacaa atggttttcc ccttaatata
    15301 ttcagctaaa acctctaaag aggcgatact agctgctcca gcaggttctg cgactatccc
    15361 ttgcttagag taaaggtcaa tcaaggtttc agaaatcaat ccctcatcga cacctaccaa
    15421 agtttgaata tgttgacgag ttgcttcata ggtcaactga cctacctttt gcacagcaat
    15481 cccatccgca aatttatcaa tttccttgag tttgactgga cctccagcct caaaggcagc
    15541 tttcatggaa cgcgctccat tcgcctctac tccgataacc tcaatctctg gacttgtttc
    15601 cttgatatag gtagaaaccc cggcaatgag accgccacca ccaacaggaa ccaagacagc
    15661 atcaaaatca atcgattctt ttcgagcttc ttctaaaatc tcataagcaa ctgttccttg
    15721 acctgcttga acatgagcat catcaaaagg atcaataaag gtacgatttt cagagactgt
    15781 aaattcttga gctgctttgg ctgaggcatc aaaggtatct ccaactagtt taatagttac
    15841 aaaatcccca ccaaaaaagc gaacctgacc aatcttttgt tgtggcgtag taatgggcat
    15901 aaagatagta gcaggaattt tcatttcatt acaagtatag gctactccct gcgcatgatt
    15961 tcccgcagaa gcgcaaacta ccccacgttc acgttcttcc ttgctgagct gggaaatggc
    16021 ataataggca ccacgaattt taaaggagcg aacacgctgg gcattttctt ttttcaaata
    16081 aatcttagca ccatacttct ccgataaata atgatcgtaa tccagtggag tattcacaac
    16141 cacaccgttc aagaccttgt gagccttgat tatatctttt gaacttaaca tataaaacct
    16201 ctttttctat aaaaaacagt cactaatgac tgttttttag ttattcatct ccgaaaattt
    16261 cagtaacact ttcatggtat ttatgagcca tacgaatcga aaactcttta ggaagttcat
    16321 aacgatttgt ggaaatccaa tcatataacc attctaccaa actttcttca ctatcattac
    16381 gatg
//
LOCUS       DAVHIT010000043        16252 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVHIT010000043 DAVHIT010000000
VERSION     DAVHIT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.044"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(1..317)
                     /locus_tag="VVN16_001218"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="VVN16_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..900
                     /locus_tag="VVN16_001219"
     CDS             442..900
                     /locus_tag="VVN16_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU5572609.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            913..1521
                     /locus_tag="VVN16_001220"
     CDS             913..1521
                     /locus_tag="VVN16_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572610.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1592..1924
                     /locus_tag="VVN16_001221"
     CDS             1592..1924
                     /locus_tag="VVN16_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572611.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1935..3350
                     /gene="ply"
                     /locus_tag="VVN16_001222"
     CDS             1935..3350
                     /gene="ply"
                     /locus_tag="VVN16_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU5572612.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            4231..4947
                     /locus_tag="VVN16_001223"
     CDS             4231..4947
                     /locus_tag="VVN16_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5572613.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            5071..5520
                     /locus_tag="VVN16_001224"
     CDS             5071..5520
                     /locus_tag="VVN16_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU5572614.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            5559..6380
                     /locus_tag="VVN16_001225"
     CDS             5559..6380
                     /locus_tag="VVN16_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572615.1"
                     /translation="MILLAILFTCFLVYLELEVPTYISKITDLLGSQETNLDELWQPA
                     SMMMGMSFLAFLSVVAVGFFASRVAASYISRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGPIMAIWAIGKILGHSEYWLWAVLVVVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIIFMSYAM"
     gene            6387..7418
                     /locus_tag="VVN16_001226"
     CDS             6387..7418
                     /locus_tag="VVN16_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5572616.1"
                     /translation="MGFLLMGALFIVFPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSVTAHAGQRVAIVGPTGAGKTTIVNLLMKFYEIDKRSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIACALLKDASLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSIIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            7484..7846
                     /locus_tag="VVN16_001227"
     CDS             7484..7846
                     /locus_tag="VVN16_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU5572617.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7843..8346
                     /locus_tag="VVN16_001228"
     CDS             7843..8346
                     /locus_tag="VVN16_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5572618.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8460..8906
                     /locus_tag="VVN16_001229"
     CDS             8460..8906
                     /locus_tag="VVN16_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572619.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8911..9585
                     /locus_tag="VVN16_001230"
     CDS             8911..9585
                     /locus_tag="VVN16_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572620.1"
                     /translation="MKKKAFGIVLLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWCVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            9837..10025
                     /locus_tag="VVN16_001231"
                     /pseudo
     CDS             9837..10025
                     /locus_tag="VVN16_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
     gene            10275..10574
                     /locus_tag="VVN16_001232"
     CDS             10275..10574
                     /locus_tag="VVN16_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU5572621.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10571..10888
                     /locus_tag="VVN16_001233"
     CDS             10571..10888
                     /locus_tag="VVN16_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU5572622.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            10904..11530
                     /locus_tag="VVN16_001234"
     CDS             10904..11530
                     /locus_tag="VVN16_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU5572623.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            11563..12324
                     /locus_tag="VVN16_001235"
     CDS             11563..12324
                     /locus_tag="VVN16_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5572624.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAETFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            12402..12797
                     /locus_tag="VVN16_001236"
     CDS             12402..12797
                     /locus_tag="VVN16_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5572625.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            13067..13351
                     /gene="groES"
                     /locus_tag="VVN16_001237"
     CDS             13067..13351
                     /gene="groES"
                     /locus_tag="VVN16_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5572626.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQS
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            13367..14989
                     /gene="groL"
                     /locus_tag="VVN16_001238"
     CDS             13367..14989
                     /gene="groL"
                     /locus_tag="VVN16_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5572627.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            15127..15882
                     /locus_tag="VVN16_001239"
                     /pseudo
     CDS             15127..15882
                     /locus_tag="VVN16_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4875 a         2959 c         3696 g         4722 t
ORIGIN      
        1 ttgaaaacat tttaaacgtt tttactttgg taaagatgtt ctcaaccttg cttctctcct
       61 tagatagcgc atggttacag gatttatctt caactgttag cggcttgagt ttgctggatt
      121 tacgtggagt ttgtgcttga ggatatatct tcatgagccc ttgataacca ctgtcagcca
      181 agattttacc agcttgtctg acttttttga taagctgttt ttaatacagc taacatctct
      241 tcaaaagtgg tacgctgaac accaacaaga cgcttaaatc gtgcatcagt tagttgttta
      301 cttgcttcat aattcataga actactatac catattttgt ttcgcaggaa gtctaataag
      361 ttatagtcca gtttttacta tactttgata ttcaaatatg ttatactatg gttatcaaag
      421 taaatgaaag gagttacgaa tatgacgcca gaagaaatgt acctgacaga gcgattagac
      481 gtacagatag ctcatttttt aaagaaaagc gttcaacatc gtaggcgcta taaggtatta
      541 aaaataacag aaatcgtggc aggtttcctc atagctgtct tttgtgctat tcctatgcca
      601 ggtgatcgct accgtttgat ttcggttgcc ttatccagtc tcggcttgct gtgtgagggg
      661 attatcaatt tgtataatgc aaaggaaaat tggatttctt accaaaaaac tgcgcaactc
      721 ctggagaaag aaaaattcct ctatcaatgc caaacggaga aatatgcagg aaagaccaag
      781 gcttttgccc tatttgtcaa gacatgcgaa ggtcttatct cagaggagat taaccagtgg
      841 gaaagtatcc agtcaaaaga agtggcagct agtgcagatg ctccagttaa aaaagagtag
      901 gaggtagagg aaatgtctca atccagctac ctgtcgccct tgctctggtt aaaaaaagaa
      961 gccgataagg aaaagatgag cgcgacccag tgccagatat ttttctttta ctatcaaatg
     1021 tttgagctct tatttgctag agaaagcgac atgaaagact tatgtctggg aacgaaaggt
     1081 ttttatttct cgcagttaga gaaaaatttg ctttctggag tttcccgatt tctaaaaaac
     1141 ttggagggga aagtaactct caaggctaac caagaagtat cagctcgcaa agcccttttt
     1201 ctagccttga caactagcca atcagattgg caggagttag ctcctgtttt tgatttttat
     1261 cagactatcg ggaggcttga aaatccttct ctcttgagtt ctcaggacag acaacatctg
     1321 atgtggattt accagtcagc tttggagaag gattatattg tcaaggttat tggtgacaag
     1381 cattttgtat tgaagagaca agatgctact aaattgacag cgcgccaaac tcaaactttg
     1441 gaaattctga gtcaatcaga agacttggtc aatcctgtct atgttacatt aggagaaaag
     1501 ggggtgctct tgcttgatta agagaggaga tgttgtagtt ctttatttgc cttttccgac
     1561 tattagtagc gatttggctg tgaagaatca tatgtatatc tgtattgaca acagcatgac
     1621 taaaaacaaa gagttggtta aaaatcagac cttcaaacca gctctcttga ccagacgttt
     1681 ggtcaagaac tttatgatag aagagccgga tctagctcgt aatcctttta caagaccaac
     1741 cttgattgac ttagataagg tatttatgtt ggataatacg gttattccga cttcttatct
     1801 agccagacgg cgacgcaatg tctcagaaga attgtacgag gaaattttgg atcacttagt
     1861 ccaaccacgg ctgatttcgc tgaacaagtc tgagtttatg caactcaatc caggaactta
     1921 ttaggaggta gaagatggca aataaagcag taaatgactt tatactagct atgaattacg
     1981 ataaaaagaa actcttgacc catcagggag aaagtattga aaatcgtttc atcaaagagg
     2041 gtaatcagct acccgatgag tttgttgtta tcgaaagaaa gaagcggagc ttgtcgacaa
     2101 atacaagtga tatttctgta acagctacca acgacagtcg cctctatcct ggagcacttc
     2161 tcgtagtgga tgagaccttg ttagagaata atcccactct tcttgcggtc gatcgtgctc
     2221 cgatgactta tagtattgat ttgcctggtt tggcaagtag cgatagcttt ctccaagtgg
     2281 aagaccccag caattcaagt gttcgcggag cggtaaacga tttgttggct aagtggcatc
     2341 aagattatgg tcaggtcaat aatgtcccag ctagaatgca gtatgaaaaa atcacggctc
     2401 acagcatgga acaactcaag gtcaagtttg gttctgactt tgaaaagaca gggaattctc
     2461 ttgatattga ttttaactct gtccattcag gcgaaaagca gattcagatt gttaatttta
     2521 agcagattta ttatacagtc agcgtagacg ctgttaaaaa tccaggagat gtgtttcaag
     2581 atactgtaac ggtagaggat ttaaaacaga gaggaatttc tgcagagcgt cctttggtct
     2641 atatttcgag tgttgcttat gggcgccaag tctatctcaa gttggaaacc acgagtaaga
     2701 gtgatgaagt agaggctgct tttgaagctt tgataaaagg agtcaaggta gctcctcaga
     2761 cagagtggaa gcagattttg gacaatacag aagtgaaggc ggttatttta gggggcgacc
     2821 caagttcggg tgcccgagtt gtaacaggca aggtggatat ggtagaggac ttgattcaag
     2881 aaggcagtcg ctttacagca gatcatccag gcttgccgat ttcctataca acttcttttt
     2941 tacgtgacaa tgtagttgcg acctttcaaa atagtacaga ctatgttgag actaaggtta
     3001 cagcttacag aaacggagat ttactgctgg atcatagtgg tgcctatgtt gcccaatatt
     3061 atattacttg gaatgaatta tcctatgatc atcaaggtaa ggaagtcttg actcctaagg
     3121 cttgggacag aaatgggcag gatttaacgg ctcactttac cactagtatt cctttaaaag
     3181 ggaatgttcg taatctctct gtcaaaatta gagagtgtac cgggcttgcc tgggaatggt
     3241 ggcgtacggt ttatgaaaaa accgatttgc cactagtgcg taagcggacg atttctattt
     3301 ggggaacaac tctctatcct caggtagaag ataaggtaga aaatgactag gagaggagaa
     3361 tgcttgcgac aaaaagaggc gatgatctct ctgcagatat ctgaaagttt atctcttgcc
     3421 tagcgatttc cattggagta tgctcgtgat gttaaaatga atctatcaaa cagaatgtgt
     3481 aaaaaagtaa aaagggtagc caattctttg atatacaggg gattgattat cctttacttg
     3541 ttttagatga ttggaaattc aagaacgaca cgtatcggaa atattttgct gtttatatct
     3601 aatacccatt tatggaaaaa atgaaattaa atatgaacaa atcaattgct agcaatggtt
     3661 tagaagtgcc agtgtactag tctaacttct ccatcttggg aacacgtaaa ttatactctt
     3721 cgaaaatctc ttcaaaccat gtcagcccca tccgcaacct caaaacagtg ttttgagcaa
     3781 cctgaggcta gctttctagt ttgctctttg atttttattg agtattagag gtttgcttag
     3841 aaactcttct attgatttct tagaatacaa tagcgcctat aagaactgtt gaatttcaaa
     3901 gtatttactt gaactatgat atgtctggag tagactgtca acaaaaggtg cagtatgatt
     3961 gtttttgtcg cttatatcta caaacttcaa acagtaattt gagctgactt tgtcaaactt
     4021 ggctgaaact ttctatttgc agataatggt ctagtttgtt ttttgaatgt ggatgagtgt
     4081 ctgttgataa agatagaaag gcaagaccat cttagcaggt aaagtgctat cctagcttga
     4141 tttgctaata aaatccattt cctctagtga aaatcaaaaa aacttgtgtt ataataagaa
     4201 agattaaaat gtgaaaaaag gagattccta atgggacgta aatgggccaa tatcgtagcc
     4261 aagaaaacgg ctaaagatgg agctaactct aaagtatatg caaaatttgg tgtagaaatc
     4321 tatgtagcag ctaaaaaagg tgatccagat ccagaatcaa actcagcttt gaaattcgtt
     4381 atcgaccgtg ctaaacaagc ccaagtgcca aaacacatta tcgataaagc gattgataaa
     4441 gccaaaggaa acacagacga aacctttaca gaaggacgtt acgaaggttt tgggccaaat
     4501 ggctctatgc taattgtgga tactttgact tctaacgtta accgtacagc agccaatgtc
     4561 cgtgcagcct ttggtaaaaa cggcggaaac atgggcgctt caggttctgt ttcttacctc
     4621 tttgacaaca aaggtgttat tgtatttgga ggtgaagatg cagacgcagt ctttgagcaa
     4681 ttgctcgaag cggatgtaga tgtggatgac gtagaagcac aagaaggtac aatcacagtt
     4741 tacacagcgc caactgacct tcacaaggct atcgttgccc tccgtgagtc tggtattgaa
     4801 gagttccaag tgactgaatt ggaaatgatt cctcagtcag aagtggaatt gtcaggcgaa
     4861 gaccttgaaa cctttgaaaa actttacagc gttcttgaag acgacgaaga cgtccaaaag
     4921 atttatacga acgtagatgg gttttaattt aacctataga acatgatcct aagtgagaaa
     4981 atcacttggg atttttttaa tcaagaaaat agttctcata gaaatttaat tattctccag
     5041 agaactattt ttatttagaa gggggagatt atggataaac cgatgttagt ctttaagcgt
     5101 tttggtcatc aaattcacct gatggtgcaa aaggaagcca aacgttgcgg cattgaattt
     5161 atgggtgggc cgcaaggtca ggttgtgcat tttttggata atcgcgagaa aaaccaagac
     5221 ttggtcttga ttaaagatat cgagcaggaa ctcaatatta ccaagtctgt tgctagtaat
     5281 ctggttaagc gtatagtgca aaatggtttg gtggaattgg aggcgagtcc tgttgataag
     5341 cgggctaagt ttgttcgtct gacggacaaa gcacgttctc agatgcaaca ggttaaggct
     5401 ttttttgaac gcatagacaa gcagttgatg gaagacattg atgaagatga attactgatt
     5461 tttgagaagg ttctcggtca actacaggca aaatatcaag ggaataggag gagagaataa
     5521 agaaattagc caaaagaatt agtagaaaag aatgggggat gattttacta gccattctct
     5581 ttacttgctt tttggtctat ctagagttgg aagtgccgac ctatatctcg aaaattacgg
     5641 atttgctagg tagtcaagaa actaatttag atgagttgtg gcagccggca agcatgatga
     5701 tgggaatgtc ctttcttgcc ttcttgtccg tagttgcagt tggatttttt gcatcccgag
     5761 tggcggcttc ttatattagt aggctgagaa gtgatatttt taaccgagtt ttggattact
     5821 cgcagacaga gattaagaaa ttttcaattc ctagcctctt gacgcgtact accaatgaca
     5881 ttactcaagt tcaaatgttg attactatgg gcttgcaagt ggtaacgcgt ggtccaatta
     5941 tggctatctg ggctattggg aagattttag gtcattcaga atactggctc tgggccgtac
     6001 ttgtggtagt gattgtcaac gtcctgatga cgaccgtttt gatgacgcta gcctttccaa
     6061 aacagtcctt gattcagggg ttgacagata aactgaacag tatcactcgt gagagtttaa
     6121 caggtattcg tgtcgttcgt gcctacaatg cagaggatta tcaaaatgaa aaatttgcag
     6181 cagtaaatga tgaattgacc cgtttgaatt tgtttgtcaa ccgtcttatg gctattttga
     6241 atcctatcat gatggggatt tcaagtggtt tgagtgtagc gatttactgg attggggcct
     6301 atgtgattaa cgacgctgct ccgatagcgc gtctgcctct ctttagtgac atgattattt
     6361 tcatgtctta tgccatgtag gttgtcatgg gcttccttct catgggagca ctcttcatcg
     6421 tttttccccg aactatggtc tctgctaagc ggattaatca agttttagat ttgcattctt
     6481 ctatccaaaa ccctgttcaa gtgcagctga ctgatgaaaa cttcaaaggt caggtcgagt
     6541 ttaaggatgt gaccttccgc tatgcggcaa attcggaggc agttattgaa catgttagct
     6601 ttaaagcaga aactggtcaa acagtggcct ttattgggtc aacaggttct ggtaaatcaa
     6661 ctctggtcaa tctgattcca cgtttctacg acgtgtcagc aggagaaatt ctggtggacg
     6721 gtgtcaatgt tcaagactat gacttctctg tgacagctca tgctggtcaa agggttgcca
     6781 ttgttgggcc gactggggct ggtaagacaa ccattgtcaa tcttttgatg aaattctatg
     6841 agattgataa gagaagtatt cgcattgatg gtgtggatac caaggctatg acgcgttcag
     6901 aagtgcatga tgccttttca atggtcttgc aggatacctg gctctttgaa ggaactattc
     6961 gagacaatct catctataat caaataggga ttagtgatga acgaatgatg gaagctagta
     7021 aggctgtggg aattcaccac tttattatga ccttgccaga tggctatgat accatcttgg
     7081 atgacaccgt gaccttgtct gtaggacaaa aacaactatt gactattgct tgtgcccttc
     7141 ttaaggatgc atcgcttttg attttggatg aggcgacttc ttctgttgac acacggacag
     7201 aggaattgat ccaaaaagcc atggaccgtt tgatggaagg acgcacatcc tttgtcattg
     7261 cccaccgctt gtcaatcatc cgaaatgcag acttgatctt ggtcatgaaa gatggaaata
     7321 tcatcgagca aggcaactat gaggaactga tggcgcaagg tggcttctac gctgacttgt
     7381 acaatagtca atttacagaa gatgaagcag aagaataaat caaaaaaagg cttgacggcc
     7441 ttctttttct tatttatact agtacacata gaaagagaga agtatgagtc agaaattata
     7501 caatatgaaa tttgctgctg tctacttagc cttgattgca aaagttgagc gaaagggtgg
     7561 aaaggcagag tcagttcacc aagtgaccag ttggttgaca gggtatgaag tgagtgacgt
     7621 tcttgcttgt ctggataggg atgttactta cggagatttt tttctacaag caccatatta
     7681 tgtccctgaa cgaattgcaa ttacagggaa gatttgcggt gtccatattg aggaaattga
     7741 tgatcctctg atgcaggaaa tacgtcgttt ggacaagcta gtggattggc ttgccaaggg
     7801 gaaaacatct caacaagttc tagaaaagta tgagaagcat aaatgaaaaa ttatcaagaa
     7861 tggtatcgaa atatcagctc cagactaact agtcgtccaa ttcttttatt cctattacgc
     7921 agtttcaatc gtttgatgac agtcgccatg cccctggtct atctgatctt gctagtcacc
     7981 acttacctgc aactaggact tggtcagcaa gttggggttt atgtgcttat tcctgcatca
     8041 ggttttgtgg ccttgtccct gtttcgtaag aaaatcaatc acccgagacc ttatgaaact
     8101 tgggatatct gtccccttct tgataaggat agttcgggac ggtcgatgcc cagtcgccat
     8161 gtcttttctg caactatcat ctccatggcc tgtctgcatg ctagtctacc tattggctta
     8221 gtatgcttga tcttttcagc tttgctgggg ttggtgaggg ttttaggggg tgttcattat
     8281 cccaaggatg tcttggttgg ctatgcttgt ggtctggtgt ggggattcct tttcttccta
     8341 ttctgacatg taagttaagc tagtcctaca accacttact gcttcaaatt gaccacttgc
     8401 ttttttgccc ttgtattcct aacttagctt tgatatagta gagtcagaaa ggagatgtga
     8461 tgaagttacg aattgagatt gacggcaatt tagaggagac tgaaattgtc atcaagaccc
     8521 ccactttgac agatgaaatt gcagacttgc aacggctctt gcaagagtca aaggctccga
     8581 ggttgacttt ttacaagggg acaggtgaat attatctaga cctgtcagaa attctcttct
     8641 ttgaaacaga agggagcaag atctacgctc ataaccagaa ggaagcttat gaggttcgcc
     8701 tcaagctcta tgagttggag tctatcttgc ctcgctattt taatcgagtt tccaagtcaa
     8761 cgatcgcaaa catccgtcag atttactcag tggacaagtc cttttcagga acgggcacca
     8821 tttcctttta tcagacgcac aaggaggttc atgtctcacg gcattaccaa tccctcctaa
     8881 aagaaaatct aagaaacatg aggtaataag atgaaaaaga aagcatttgg tattgtgtta
     8941 cttgtactag cagctttaat attagtacaa ggaaattttg gaatcccttc tctaggaggg
     9001 cacatttggc ccttgattgg tattgctttt tttgcctacc aatcagttgg agctttgttg
     9061 cgtcgccact taacttcagc ctcttttaca gctctagtag ccttaatgat tgctaaccac
     9121 ttttatggca ttttaccgat tccaaaccag acactaatct gggcaggagt tctaatcgtt
     9181 ctcggagtaa atatgttgac tcattctaac agaacttgga atgggaaaaa atggtggtat
     9241 gatggtgaaa aaaacattct tatagataag gaagttgctt ttgggacagg gactttctac
     9301 aagcaagacc aagaattggt agaagaccaa gtagaagttg gttttggcaa tgcaaaaatc
     9361 tactatgaca atgcagagat gttaggtgat tttgcaactt taaatattga agtagctttt
     9421 ggtaatgcaa ctgtctatgt tccgcaacac tggtgtgtcg atttgaaagt agaaacatcc
     9481 tttggtgcag ccaaggcgga cgctcctgta gcgccaacaa gcaaaacctt gattattcgt
     9541 ggagacgtgg cctttgggaa gttggagctt gtctacgtta aataaaaaaa tcttcactta
     9601 aaccatcaaa atagacgtac taagagtagg aaattgatgc cttgctctga tttcagttct
     9661 atggttgtta gactttaaaa aatgaaatgt tgcctttaaa agttgtatat ttttcgatat
     9721 tttggctctt acgtttgatg tatctatgtc ctacagcgtg gatgatgtag tgtaaaatgc
     9781 tttaaaaaaa tgaatgatat tggacagttt tttgaacctt taattgctca ggagccatga
     9841 aagcaagcac ttgagtttat gacaagggag aatggtatta tgtaagttct tctggagcca
     9901 tgctagcgaa tgattgggtc aaagataatg gcaagtggta ttatctagct tcatcaggta
     9961 agatgcttcg caatacctac acacctgatg gttactacgt tggtaactca ggcgcctggc
    10021 aataagaata atactcttcg aaaatctctt caaaccacgt cagcttcatc cacaacctca
    10081 aaacagtgtt ttgagctgac ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag
    10141 cagcctgcgg ctagcttcct agtttggtct ttgattttca ttgagtataa gaagcccttt
    10201 tccttaaagg aagaggcttt ttacttgctt ttttgctgtt tcctcatttg tcctaaagcc
    10261 ttttttgtgt taaaatgaat ggtatgaaag ctaaaaaaat gtggatggca ggtctggctc
    10321 tgcttggtat tgggagcctt gcccttgcta cgaaaaaagt tgcagatgat cataagctca
    10381 tgaagactca ggaagagttg acagcgattg tacgagacca tttttcagac atgggggaaa
    10441 ttgcgaccct ctatgttcaa gtctatgaaa gcagtctaga gagcttgctt ggtggcgtca
    10501 tttttgagga tggccgtcat tatacttttg tctatgaaaa tgaagaccta gtctatgagg
    10561 aggaagtctt atgatacaac cagcaagttt agaagaattg gcatctttag tagaaaaaga
    10621 cggtaagaag gtcttccttt ttgtggcgga ctggtgtggc gattgccgtt atatctatcc
    10681 tgccttacca gaaattgagg agactaatcc agagtttacc tttattcgag tggatagaga
    10741 ccagtatata gatttggcca aactctggga tgtatatgga atccctagcc ttgttgttct
    10801 agaaaaggac aaggaaatcg gtcgttttgt caatcgcgac cgtaaaagca agcaacaaat
    10861 caatgacttt ttagcaggac tgaaatagga gaaaaaggaa acaatgattt ttacatataa
    10921 taaagaacat gtcggtgatg tccttatggt catcgtgaaa aatagcggag atgccaaact
    10981 ggatgtggag cgcaaaggca aggtagcccg tgtttttctc aaagataatg gggaaacagt
    11041 ggcttggaat attttcgaag tttcaagttt atttgaaatt gcagagcgcg gtcaagtctt
    11101 tttgacagat gagcaagtcg ctcgtttgaa ccaagagttg caggcggaag gttttacaga
    11161 agaaattgtt aatgataagg aacctaagtt tgttgtcggt gaaattgttg agatggtagc
    11221 ccatccagat agtgaccacc tcaacatctg ccaagttgca gtcgcaagtg ataagactgt
    11281 acaaatcgtt gcaggagctc ccaatgcgcg tgtggggttg aaaaccattg tcgcgcttcc
    11341 tggagctatg atgccaaaag ggaatctcat tttcccaggt gaacttcgtg gtgaaaagag
    11401 ttttggtatg atgtgcagtc ctcgtgaatt gcatctgcca aatgctccac aaaaacgtgg
    11461 ggtgcttgaa ttatcagaag accaagttgt cggaacaccg ttcgacccag cgaaacactg
    11521 gactgcctag gaacttgtca gcatctgata gaaggaaata agatggcaaa aaatgtagtg
    11581 attacgggag caacctcagg aatcggtgaa gcgattgcgc gtgcttatct ggagcagggt
    11641 gaggatgtcg ttctaacagg acgacggata gacagattag aaatcctcaa gtcggagttt
    11701 gcagaaactt ttccaaatca aaccgtctgg acttttccac tagatgtgac ggatatggtc
    11761 atggtgaaga ctgtttgctc tgatattcta gaaacgatag ggaggattga tatcttggtc
    11821 aacaacgccg gactggctct tgacttggct ccctatcaag actatgagga gttggatatg
    11881 ttgaccatgt tggataccaa tgttaaaggt ctgatggcgg ttactcgctg tttcttgcca
    11941 gcaatgataa aagtcaatca aggtcatatt atcaatatgg ggtcaaccgc aggaatctac
    12001 gcctatgctg gtgccgctgt ttactcagct accaaggcag cggttaagac cttttcggat
    12061 ggactgcgaa ttgataccat cgcaacggat atcaaggtga caaccattca gcctgggatt
    12121 gtcgaaacag atttctcaac tgttcgtttt catggtgata aagagcgggc tgcgtccgtt
    12181 taccaaggaa tagaagcctt gcaagctcag gatattgcag acacagtagt ctatgtgacc
    12241 agtcagcctc gccgtgttca gattacagat atgaccatta tggccaatca acaggcgaca
    12301 ggtttcatga ttcataaaaa ataagaaatt tcctcgaaaa gttacaaatt tctgtaactt
    12361 tttttgattt cctacgaata gataagtagg aggaagaaaa tatgtataat aaagttatca
    12421 tgattgggcg tttaacgtct acaccagaat tgcacaaaac caacaatgac aagtcggtag
    12481 cgcgagcaac tatcgctgtg aaccgtcgtt acaaagacca aaacggtgaa cgtgaagctg
    12541 attttgtcaa tatggtccta tggggcagac tagcagaaac tttggcaagc tacgcaacca
    12601 aaggtagtct catttccgtt gatggagaat tgcgtacccg tcgctttgag aaaaatggcc
    12661 aaatgaacta cgtgaccgaa gtacttgtca caggattcca actcttggaa agtcgtgctc
    12721 aacgtgctat gcgtgaaaat aatgcaggcc aagatttggc agatttggtc ttggaagagg
    12781 aagaattgcc attttaatac tcttcgaaaa tctcttcaaa ctacgttagc tttatccaca
    12841 acctcaaagc agtgctttga gcagcctgcg gctagcttcc tagtttgctc tttgattttc
    12901 attgagtata aacattaaaa agtctgagtt ggtctcaggc tttttatctt gagaaagtca
    12961 gacttttttc ttgactattt ctgaccaagt gatacaatag aattatgaat tagcactcaa
    13021 gttcaaagag tgctaataat atctatctca ttatggagga aatcagatgt tgaaaccatt
    13081 aggggaccgt gtggtcttaa aaatagaaga aaaagaacaa accgttgggg gctttgtcct
    13141 tgcaggctca gcccaagaaa aaaccaaaac agcccaagtt gtggctactg gacaaagtgt
    13201 tcgtaccttg aacggtgact tggttgctcc aagtgttaaa actggagacc gtgtcttagt
    13261 tgaagcccac gcaggtcttg atgtcaaaga tggcgatgaa aagtacatca tcgtaggcga
    13321 agctaacatc ttggcaatca ttgaagaata gaaggagaaa gtaagtatgt caaaagaaat
    13381 taaattttca tcagatgccc gttcagctat ggtccgtggt gtcgatatcc ttgcagatac
    13441 tgttaaagta actttgggac caaaaggtcg caatgtcgtt cttgaaaaat catttggctc
    13501 accattgatt accaatgacg gtgtgaccat tgccaaagaa atcgaattag aagaccattt
    13561 tgaaaatatg ggtgccaaat tggtatcaga agtagcttca aaaaccaatg atatcgcagg
    13621 tgatggaact acaactgcaa ctgttttgac ccaagcaatc gtccgtgaag gaatcaaaaa
    13681 cgtcacagca ggcgcaaatc caatcggtat tcgtcgtggg attgaaacag cagttgccgc
    13741 agcagttgaa gctttgaaaa acaatgccat ccctgttgcc aataaagaag ctatcgctca
    13801 agttgcagcc gtatcttctc gttctgaaaa agttggtgag tacatctctg aagcaatgga
    13861 aaaagttggc aaagacggtg tcatcaccat cgaagagtca cgtggtatgg aaacagagct
    13921 tgaagtcgta gaaggaatgc agtttgaccg tggttacctt tcacagtaca tggtgactga
    13981 tagcgaaaaa atggtggctg accttgaaaa tccgtacatt ttgattacag acaagaaaat
    14041 ttccaatatc caagaaatct tgccactttt ggaaagcatt ctccaaagca atcgtccact
    14101 cttgattatt gcggatgatg tggatggcga ggctcttcca actcttgttt tgaacaagat
    14161 tcgtggaacc ttcaacgtag tagcagtcaa ggcacctggt tttggtgacc gtcgcaaagc
    14221 catgcttgaa gatatcgcca tcttaacagg cggaacagtt atcacagaag accttggtct
    14281 tgagttgaaa gatgcgacaa ttgaagctct tggtcaagca gcgagagtga ccgtggacaa
    14341 agatagcacg gttattgtag aaggtgcagg aaatcctgaa gcgatttctc accgtgttgc
    14401 ggttatcaag tctcaaatcg aaactacaac ttctgaattt gaccgtgaaa aattgcaaga
    14461 acgcttggcc aaattgtcag gtggtgtagc ggttatcaag gtcggagccg caactgaaac
    14521 tgagttgaaa gaaatgaaac tccgcattga agatgccctc aacgctactc gtgcagctgt
    14581 tgaagaaggt attgttgcag gtggtggaac agctcttgcc aatgtgattc cagctgttgc
    14641 taccttggaa ttgacaggag atgaagcaac aggacgtaat attgttctcc gtgctttgga
    14701 agaacccgtt cgtcaaattg ctcacaatgc aggatttgaa ggatctatcg ttatcgatcg
    14761 tttgaaaaat gctgagcttg gtataggatt caacgcagca actggcgagt gggttaacat
    14821 gattgatcaa ggtatcattg atccagttaa agtgagtcgt tcagccctac aaaatgcagc
    14881 atctgtagcc agcttgattt tgacaacaga agcagtcgta gccaataaac cagaaccagt
    14941 agccccagct ccagcaatgg atccaagtat gatgggcggg atgatgtaag ctttctatag
    15001 aaaacaactt ataaaaaaca caaaaggagg gaatgactaa cccttctttt tataatattc
    15061 acttctaaat tgatttgagc tctcctaact tatatgataa aataagacta gaaaaaggag
    15121 aagaacctga tcgatgtaga agaaattctg tgcaagatga cccccaatca gaagattaat
    15181 tatgaccgtg tcatgcagaa aatggtacaa gtatgggaga aaaatgagca gcggccaacc
    15241 attcttgtgc atgtttgctg tgccccttgt agtacctata cactagaata tttgaccaag
    15301 tatgcagatg tgaccatcta ttttgccaat tctaatatcc atcccaaggt agaataccat
    15361 aagcgggtct atgtcaccaa gaaatttgtt agtgatttta atgagcggac aggaaatacg
    15421 gttcagtacc tagaagctcc ctacgaaccc aattaatacc gaaaactagt taggggacta
    15481 gaggaggagc ccgaaggtgg cgaccgttgc aaggtttgtt ttgactaccg actggataaa
    15541 acagcgcaag tggctatgga cttgggcttt gactactttg gttcagcctt gaccatcagt
    15601 cctcataaga attctcaaac catcaatagc atcggaatcg atgtgcaaaa aatttacacg
    15661 acccactatc ttcccaacga tttcaagaaa aatcaaggct acaaacgttc agtagagatg
    15721 tgtgaggagt atgatatcta tcgtcaatgt tattgtggct gcgtctatgc agcccaagcc
    15781 cagaatattg acctggttca agttaagaag gacgccacag catgcttgtt ggataaggat
    15841 gttgagaaag actattctca tatcaaattt atagtagatt gaaactagaa tagtacacct
    15901 ttacttctaa aacattgtta gaaatcgatt tgactgtcct tatttcattt tactatacta
    15961 ttacgaaatt agatatataa atgaaaactt gcctcaaggt aggttttttg ctagtagaaa
    16021 agcgatagac agaaattgtt ggactcttaa aaaaatgaag ctttgctcaa aagagcttct
    16081 taaattaaaa cgagcaatta tcagttcaaa tctgtttcaa aagaagaacg gataaattaa
    16141 tcgatgcttt gtttttgagt taggaagtta agcatgccaa actgagtcgt aggaataaat
    16201 ttctttatag aaatgtgtta caactctgct ataacggaat attccttttt tg
//
LOCUS       DAVHIT010000044        16071 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVHIT010000044 DAVHIT010000000
VERSION     DAVHIT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.045"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            95..2260
                     /locus_tag="VVN16_001240"
     CDS             95..2260
                     /locus_tag="VVN16_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU5572628.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2260..3000
                     /locus_tag="VVN16_001241"
     CDS             2260..3000
                     /locus_tag="VVN16_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5572629.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3149..4636
                     /locus_tag="VVN16_001242"
     CDS             3149..4636
                     /locus_tag="VVN16_001242"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5572630.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(4680..5969)
                     /gene="ftsY"
                     /locus_tag="VVN16_001243"
     CDS             complement(4680..5969)
                     /gene="ftsY"
                     /locus_tag="VVN16_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5572631.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEADEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTIEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5973..6791)
                     /locus_tag="VVN16_001244"
     CDS             complement(5973..6791)
                     /locus_tag="VVN16_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5572632.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYADVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6791..7585)
                     /locus_tag="VVN16_001245"
     CDS             complement(6791..7585)
                     /locus_tag="VVN16_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5572633.1"
                     /translation="MTIKLVATDMDGTFLDENGRFDMDRLKSLLVSYKEKGIYFAAAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYESTMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            complement(7582..11121)
                     /gene="smc"
                     /locus_tag="VVN16_001246"
     CDS             complement(7582..11121)
                     /gene="smc"
                     /locus_tag="VVN16_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU5572634.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETFWKLQEEEIDRLSEGDWQADKEKCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHSRLEFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11112..11810)
                     /locus_tag="VVN16_001247"
     CDS             complement(11112..11810)
                     /locus_tag="VVN16_001247"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU5572635.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(11993..12064)
                     /locus_tag="VVN16_001248"
     tRNA            complement(11993..12064)
                     /locus_tag="VVN16_001248"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12029..12031),aa:Arg,seq:ccg)
     gene            complement(12128..13114)
                     /locus_tag="VVN16_001249"
     CDS             complement(12128..13114)
                     /locus_tag="VVN16_001249"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU5572636.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13289..14605)
                     /locus_tag="VVN16_001250"
     CDS             complement(13289..14605)
                     /locus_tag="VVN16_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU5572637.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFFVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKTEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14592..>16071)
                     /locus_tag="VVN16_001251"
     CDS             complement(14592..>16071)
                     /locus_tag="VVN16_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU5572638.1"
                     /translation="IGRSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGP
                     YLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSL
                     LKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSN
                     GDGAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSY
                     LSVNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFG
                     LKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLH
                     ETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHA
                     EEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLR
                     GSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
BASE COUNT         4610 a         3577 c         2910 g         4974 t
ORIGIN      
        1 caatgttata ttttctgaca cgaaaaatat aaaaatttgc ctttttttac tttttctgat
       61 ataatgagag aatattcgga aaaggagact aaaaatgaag aaaaaatttc tagcattttt
      121 gctaatttta ttcccaattt tctcattagg tattgccaaa gcagaaacga ttaagattgt
      181 ttctgatacc gcctatgcac cttttgagtt taaagattca gatcaaactt ataaaggaat
      241 tgatgttgac attattaaca aagtcgctga gattaaaggc tggaacattc agatgtccta
      301 tcctggattt gacgcagcag tcaatgcggt tcaagctggg caagccgacg ctatcatggc
      361 agggatgaca aagactaaag aacgtgaaaa agtcttcacc atgtctgata cttactatga
      421 tacaaaagtt gtcattgcta ctacaaagtc acacaaaatt agcaagtacg accaattaac
      481 tggcaaaacc gttggtgtta aaaacggaac tgccgctcaa cgtttccttg aaacaatcaa
      541 agataaatac ggctttacta ttaaaacatt tgacactggg gatttaatga acaacagctt
      601 gagtgctggt gccatcgatg ccatgatgga tgacaaacct gttatcgaat atgccattaa
      661 ccaaggtcaa gacctccata ttgaaatgga tggtgaagct gtaggaagtt ttgctttcgg
      721 tgtgaaaaaa ggaagtaaat acgaacacct ggttactgaa tttaaccaag ccttggctga
      781 aatgaaaaaa gatggtagtc ttgataaaat tatcaagaaa tggactgctt catcatcttc
      841 agcagtgcca actacaacca ctctcgcagg attaaaagct attcctgtta aggctaaata
      901 tatcattgcc agcgattctt cttttgcacc ttttgttttc caaaattcaa gcaaccaata
      961 cactggtatt gatatggaat tgattaaggc aatcgctaaa gaccaaggtt ttgaaattga
     1021 aatcaccaac cctggttttg atgctgctat cagtgctgtc caagctggtc aagccgatgg
     1081 tatcattgct ggtatgtctg tcacagatgc tcgtaaggca acttttgact tctcagaatc
     1141 atactacact gctaatacca ttcttggtgt caaagaatca agcaatattg cttcttatga
     1201 agatctaaaa ggaaagacag tcggtgttaa aaacggaact gcttctcaaa ccttcctaac
     1261 agaaaatcaa agcaaatacg gttacaaaat caaaaccttt gctgatggtt cttcaatgta
     1321 tgacagttta aacactggtg ccattgatgc cgttatggat gatgaacctg ttctcaaata
     1381 ttctatcagc caaggtcaaa aattgaaaac tccaatctct ggaactccaa tcggtgaaac
     1441 agcctttgcc gttaaaaaag gagcaaatcc agaactgatt gaaatgttca acaacggact
     1501 tgcaaacctt aaagcaaacg gtgaattcca aaagattctt gacaaatacc tagctagcga
     1561 atcttcaact gcttcaacaa gtactgttga cgaaacaacg ctctggggct tgcttcaaaa
     1621 caactacaaa caactcctta gcggtcttgg tatcactctt gctctagctc ttatctcatt
     1681 tgctattgcc attgtcatcg gaattatctt cggtatgttt agcgttagcc catacaaatc
     1741 tcttcgcgtc atctctgaga ttttcgttga cgttattcgt ggtattccat tgatgattct
     1801 tgcagccttc atcttctggg gaattccaaa cttcatcgag tctatcacag gccaacaaag
     1861 cccaattaac gactttgtag ctggaacaat tgccctctca ctcaatgcgg ctgcttatat
     1921 cgctgaaatc gttcgtggtg gtattcaggc cgttccagtt ggccaaatgg aagccagccg
     1981 aagcttgggt atctcttatg gaaaaaccat gcgtaagatt atcttgccac aagcaactaa
     2041 attgatgttg ccaaactttg tcaaccaatt cgttatcgct cttaaagata caactatcgt
     2101 atctgctatc ggtttggttg aactcttcca aactggtaag attatcattg ctcgtaacta
     2161 ccaaagtttc aagatgtatg caatccttgc tatcttctat cttgtaatta tcacactttt
     2221 gactagacta gcgaaacgct tagaaaagag gattcgttaa tggcaaaatt aaaaattgat
     2281 gtaaatgatt tacacaagca ctatggaaaa aatgaagtcc taaaaggaat tacgactaag
     2341 ttctatgaag gagatgttgt ttgtatcatc ggtccttcag gttctggtaa gtcaactttc
     2401 ctccgtagcc tcaatctttt agaagaagtc actagcggtc acatcactgt gaacggctat
     2461 gatttaactg aaaaaacaac caatgttgac cacgtccgtg aaaatatcgg catggtattc
     2521 caacacttca acctcttccc tcatatgtct gtattggaca acatcacctt tgctcctatt
     2581 gagcacaagt tgatgactaa ggaagaagct gaggaattgg gaatggagtt gcttgaaaag
     2641 gttggactag cagataaagc taatgccaat ccagatagcc tatcaggtgg tcaaaaacaa
     2701 cgtgtggcca tcgctcgtgg cctagcaatg aatccagaca tcatgctctt cgatgaacca
     2761 acttctgccc ttgaccctga gatggttgga gacgtactta acgttatgaa ggaattggct
     2821 gagcaaggca tgaccatgat tatcgtaacc catgagatgg gatttgctcg tcaggttgcc
     2881 aaccgcgtta tctttactgc agatggcgag ttccttgaag acggaacacc tgaccaaatc
     2941 tttgataacc cacaacaccc tcgtctgaaa gagttcttag ataaggtctt aaacgtctaa
     3001 actcaaactg taaggatttc cttgcagttt ttctacctcg tattggaatt tttgattttt
     3061 cggaaaatta tgttagaatt aagtttatga aatgaggttt cctcatacct agcaagacta
     3121 ggaataaaaa tagaaattag gtagctagat gtcatctaag gttattgtta caattttcgg
     3181 tgcgagtgga gacctggcta aacgcaagct ctacccttcc ctttttagac tatataaatc
     3241 cggcaatctt tccaagcact ttgccgttat tggaactgcc cgtagacctt ggagtaagga
     3301 atattttgaa tctgtagttg tcgagtccat ccttgatttg gcagatagta ccgagcaagc
     3361 ccaagaattt gctagccact tctactatca aagccatgat gtcaatgatt cggaacatta
     3421 tattgctttg cgtcaattac aagctgagct taatgaaaaa taccaagctg aacacaataa
     3481 gctcttcttc ttgtctatgg cacctcagtt ctttggaacc attgccaaac acctcaaatc
     3541 tgaaaacatt gtcgatggca aaggttttga gcgcttgatc gttgaaaaac catttggtac
     3601 agattacgca actgcaagca agttgaatga cgaactccta gcaacatttg acgaagaaca
     3661 aattttccgt atcgaccatt atcttggtaa ggaaatgatc caaagcatct ttgcagttcg
     3721 ctttgcaaac ttgatttttg aaaacgtttg gaacaaggat tttatcgaca atgttcaaat
     3781 tacctttgcg gagcgcttgg gtgtagaaga acgtggtggc tactatgacc aatccggtgc
     3841 cctccgtgac atggtccaaa accacactct acaacttctt tcgctcctcg ctatggacaa
     3901 accagcaagc ttcacaaaag acgagattcg tgctgaaaag attaaggtct ttaaaaacct
     3961 ctatcatcca actgatgaag aactcaaaga acactttatc cgtgggcaat accgctctgg
     4021 taagattgat ggcatgaaat acatctctta tcgtagcgaa ccaaatgtga atccagaatc
     4081 aacaactgaa acctttacat ctggtgcctt ctttgtagac agcgatcgat tccgtggtgt
     4141 tcctttcttt ttccgtacag gtaaacgact gactgaaaaa ggaactcatg tcaacatcgt
     4201 ctttaaacaa atggattcta tctttggaga accacttgct ccaaatattt tgaccatcta
     4261 tattcaacca acagaaggct tctctcttag cctaaatggg aagcaagtag gagaagaatt
     4321 taacttggct cctaactcac ttgattatcg tacagacgcg actgcaactg gtgcttctcc
     4381 agaaccatac gagaaattga tttatgatgt cctaaataac aactcaacca actttagcca
     4441 ctgggatgaa gttggtgcgt catggaagtt gattgatcgt attgaaaagc tctgggctga
     4501 aaatggtgcc ccacttcatg actataaagc tggaagcatg ggacctcaag ccagctttga
     4561 cctacttgaa aaattcggtg ccaaatggac ttggcaacca gatatcgcct atcgtcaaga
     4621 tggtcgtttc gaataaaaaa atcctgcaag tttgtacctt gcaggatttt tgcttctgat
     4681 tagattaagc cttccaagag acctttcata aagttttctg agttaaactc tccaatatca
     4741 tcgatttttt caccaaaacc aatcaatttt acaggaatat tgagttcttc acgaatggct
     4801 agaaccacac ctcctcgagc agttccatca atcttagtca aaacaattcc cgttaaaggt
     4861 gtgattttcg aaaattcttt ggcctgtact agggcatttt gacctgttga tgcatcaagt
     4921 gccaagaagg tttcatgtgg tgcttctggc acaacacgtt tgataatacg accaatcttt
     4981 tccaactcag ccataaggtt atccttattt tgcagacgac cagcagtatc aatcatgaga
     5041 atatcgatac cttcagccac ggcacgttcc ataccatcaa agaccacgct ggctggatca
     5101 gctttttcag gtccagttac tactggaaca tctactcgtc ggccccattc agctagctga
     5161 gctactgcac ccgcacggaa ggtatctgct gcaaccagca tgaccttctt accagttcgt
     5221 ttgtagcggt gggctagttt tccaatagaa gttgttttcc caacaccatt cacaccaaca
     5281 aagagcataa ctgtcaagtt atcttggaag tggatgcttt catcgtagct accatccttt
     5341 tcataaagct caaccaattt ctcaatgatg acacgacgaa gtgcatcagg tttcttggca
     5401 ttttcaagct tggcttcgta acgtagttcc tccgttaagt tagaagcgac ttggacacca
     5461 acatcactca taatcagcag ttcttccagt tcctcgaaaa attcttcgtc aacagagcgg
     5521 aagttagcaa agaaggcgtt caagcgagca ccgaagcccg tacgagtctt cttgagactg
     5581 cggtcatatt tttcctgaac agtttcttct gtttgaggcc tttctggttc aagaacttca
     5641 gaattatttt cttctatagt tccttcgtgc tcaagcttct cttcctctgg taattcttct
     5701 gagtttggta attcttctat ttcttcttga gaaaccccta cagctggctc tgaatcctga
     5761 ctttcttcaa ctgtgtcttg gatttcctcc tcttggaaca cagcttgttc aacaatttca
     5821 acctctgctt cttcctgagg aacttcatca gcttctgtga aggtaggatc aacatcttca
     5881 gacaaatcaa gattttccag agcttctttt acaacttctt cgattttagg ttcttctttt
     5941 tttccgaata gacggtcaaa caatcccata tcttagttct cctttagcac atattcttcg
     6001 atagcccagg cgacagcttc ctcatcgttg gtcatcggcg ttactacatt tgcggctgcc
     6061 tttacttcag gaacagcgtt ttgcatagca acaccaagac ctgcccattc aatcatagaa
     6121 aggtcattgg cctcgtcacc acaggccata acttggcttt ggttgattcc aagatggctg
     6181 attagttttg ccaaacctgt tgctttatga acattctttg gtgaccactc tagcaacatt
     6241 tcacgtgatt taaagatttc atattggtca aacaattctg gagaaatctt ctgaatggct
     6301 gcatccaagg gttcttgagc aaaggcagtc acgcatttgt tgtaggtcat ttgactagac
     6361 aggtcttcaa agtcaactgg aacaaaagtc aaagctggat tgaatttggc ataaagactt
     6421 tcttggtccg attggatttg ataaactgtt ccttctgaga tggcatcaag tggcagtgat
     6481 aatttctctg ttttctcata caagcgtgcc acatcagcat atgaaaagac tgttttatca
     6541 aggatttcac ctgtattttt ctgaaccaaa ccaccattaa aagtaatggt atactcatct
     6601 tcctgaccgt cagtccctaa ctcatggaga aagaagtcca tggcttttaa gggacgacca
     6661 gttgtcaata cgaccttgat accacgatca cgcgcagctt tcaaagttgc cttggtgcga
     6721 tccgtcagcc ttttatcagt agtcagcaag gtaccgtcca agtccaatgc gattaatttt
     6781 atatctgcca ttataagccc tccatataag ctataaccga ctggtcctta tggtgaccaa
     6841 tcacagtctt tgctaattct aaaatctctg gtcgtgcatt ttcaggagct acaggatgtc
     6901 ccacaacctg catcatatgt aagtcattaa gattgtctcc aaaagccatg acctgatcca
     6961 ttgtgatgcc aagtttttta gctaattcaa caatagctac acccttatcg acatagtcca
     7021 gaacaatatc aatagattca aagccagttg tcatagcctt gacaccaggg acattatcat
     7081 tgacccaagc ttcaccagct tctagcgttt cttctgtgaa gttggttgta aatttgaaaa
     7141 tgtcatctgt gatatcttcc aaactcgcta ctttttggat attttcatta taatgctgac
     7201 tcactttcaa ataggtctca tcaaccgtat ctagaacata tgaacccttc ttacccgtca
     7261 agagcagttt attgatatct atataaggtg acgttttcag cttttcaaaa gttgccagat
     7321 aaaagtcacg agacatagtc gattcataca agtcctgacc ttgatactct accaaactgc
     7381 cattttccgc gatgaaaata atgtcatcac gaacaccagc aaataatttt tccagagaca
     7441 gaaatccccg acccgaagcc gccgcaaagt aaatcccttt ttccttgtag gaaaccaaga
     7501 gagacttgag gcggtccata tcaaagcgcc cattctcatc taggaaggtt ccgtccatat
     7561 ccgttgctac tagtttaatt gtcatccttc aatactttct aaatctttta acttaactga
     7621 aacaatcttt gaaacacccg attcttgcat ggtcactcca tagatggaat cggccgctgc
     7681 catggttccc ttacggtggg ttacgacgat aaactggctg tccttgtcaa agcggttgag
     7741 gtaatcccca aaacgtttaa cattggcttc atccaacgca gcttccacct catccaagat
     7801 gacaaaagga atggtcttga cacgaataat ggagaaaagc aaggcaagag ccgatagggc
     7861 tttttcacca ccactcatga ggttaagcga ctggattttc ttacctggag gttgaacaga
     7921 aatctccaca ccagctgtta aaaggtcgcc ctcagtcaat atcaagtctg cctgacctcc
     7981 gccaaacatc tgcttgaagg tcactttaaa ggactcacga atagcttcaa aggttgattt
     8041 aaagcgttcc ttgacctcat cattcatctc tgtaatggtc tcaaggagca gatttttcgc
     8101 tgacaaaata tcatctcgct gactatttag aaattccaga cggctgtgaa cttcttcgta
     8161 ctggtcaata gcttccaagt tgacaggtcc cagtgagcga atagccttct ctaaatcctg
     8221 cacctcttgc tctgccagat tgagattttc caactcatgc gctttttcta gtgcttcagt
     8281 atagctaatc tggtactggt ctgttaattg actttgtaga tggcgcaagc gctcgctgac
     8341 cttttctttc ttagcttcag cacgtgtttg cttgcgaatc cactcctcat tctgctggcg
     8401 agcctgatcc aaatgactag caatatcatc cagctgacct tctatatcat ccaactcaaa
     8461 ctgtttgcga atcaaacctt gttggaggtt cgttttctga gttttggatt cttccgcctg
     8521 ttgactgagc aattctgtat caaccttctc aagattgtca accttttctt gaagaaggcg
     8581 ctggatttcc tcttgttcaa aatcaagatt gtccaattcc ttgcctaagc gttcaatatc
     8641 agcaacttca taacgttttt gcccttgcag ttctgtctta agcaaacgag cttgcgctag
     8701 ctcttcctgc aagttttgat agcgttcttg gatggcattt ttattagact taatctcttc
     8761 aatctcagct tccagatttt gcttgtcact ggcgattgca gcaagacgct cttggcattt
     8821 ttccttatcc gcttgccaat ctccctcgga aagacgatct atttcctctt cttggagttt
     8881 ccaaaaagtt tccagttctt caacttgctg actagtttgc tgataagcga gggacaagcc
     8941 ttgctcctga atacgtgcct gctctccttg agatttaata gcttctaatg actcggtcaa
     9001 tctggccatc tcatcttgca aggtcttcaa agccacttct tctgaaccca agcttgcttc
     9061 ttcttcagca atttcttttt gtaattgctc cagttctggc ttgatgaaga tgctgttgtt
     9121 ctgacgattg gctccacctg cataggaacc acctgtacga agctctgtcc catccaatgt
     9181 caccatgcga acctgataac gaacttgtcg agcagcttca cgcgcatgtt ctacggtatc
     9241 aaagatagcc gtcgtagcta gcaagttctt gaaaatggct tccagtctag tatcaaaagt
     9301 caccaactca tctgccatcc caaggaaacc tgggcttaca gcgatagcat cttggttctg
     9361 actagaaatc gtacgcgcct taatagtggt caaaggaaga aaggttgcac gaccggctct
     9421 gtttcgtttg aggaaatcaa tagctttggt tgccgactct tcatcttcta cgatgatatg
     9481 ctggctactt gcccctaagg caatctctag ggcagtttga taataaacat caaaggtcag
     9541 atgctcactg actgcaccaa taatcccacc taggcgatct ttttcttgga gaacactctt
     9601 aacacctgca taaaagttac tatgatttct caggatattt tccaaacttt gagctctggc
     9661 ctgcttgttt ttgagattat ccagacggtc aaagagttgg ctttgttgag cttgatagga
     9721 agttttctgc tcctcttgct ccttggcaat agcttggtag tcagccaata atttctgaac
     9781 ctgctccttg gcagtttcaa gctcttcctt ttgctgacta gccttctctt tagctatagc
     9841 taattgctct ttcagctttt ctagttgatc tgcttgtttt tgagaaagct gacgactatt
     9901 ttccaactca ttctcaatac gggtcaactg gtttgagaca tccgcttctt cttgtaaaag
     9961 agctacaaag cgttcacgta agagctcaat catctgatca ggatcgtctg agaaagccag
    10021 caattcagct tctaaacgat tgagtttttg attattttgg actagatttc cctctaacag
    10081 agctaaagag ctttctttat cagacttttc tttgctgagt gaatttctct tatcctccaa
    10141 agcagccaaa cgggcttgtg cctcctgttg attcagggcc acttgctcgg actccagttt
    10201 cgatagggct aattttcttt ctaaatcact aatcagacta gtcaagtcca tcaaactgcc
    10261 ttggtctttg gccatttcag cctgtaaatc ttggcgttgc tttttaagag tttgattttc
    10321 ttcttctaat ttttcacgct tttggtaata actcatcaag agttcttgaa cctgagccaa
    10381 ctcttcttct gtcgactcta gttctgcctt attttccttg atttgagcaa ccagaacgtc
    10441 taaataaata gccttacgtt gtccttccaa gtctaaaaac ttacgggcat tctcagcttg
    10501 cttctcaaga ggcttgattt gattatccaa ctcgtagata atgtcctcta agcggtccag
    10561 attatcctga gtttgttgca gtttactctc agtttctttt ctacgagtct tgtattttaa
    10621 aactcctgca gcttcttcaa aaatagctcg gcgttcctca ggcttagaat taaaaatctc
    10681 ctcaaccttg ccttgggaaa tgatagagaa ggaatctcgt cccaatccag tatccaagaa
    10741 gaggtcatga atatcacgca gacggacttt cttgccgtca atcttgtatt cgctatctcc
    10801 actacgatag atatggcgtt ccaccctgat ttcttgacct gcatccttga taaatccgtc
    10861 atgattatcc agagtcacaa ctacagaagc ataattgagc ggtttgcgac tttcggttcc
    10921 agcaaagatg acatccggca tcttgccccc acggagactc ttgacactag actcccccaa
    10981 agcccaacgc agactttctg taatattgga ctttccagat ccattgggtc caacaactgc
    11041 cgtcacacct tggtcaaaaa cgaccttggt cttatcagca aaagacttga acccctgaat
    11101 ttcgatttcc tttaaataca tgaatccagc cccttctcaa cggcattttt ggcagcttcc
    11161 tgctctgcta atttcttaga acgaccttgg ccttgaccga tgctcttacc ttcaacaaga
    11221 acttctacat caaagacctt atcgtgagca ggccctgttt cagaaatcac ctgataacga
    11281 atagccacat caccattgac ctgaagcaac tcttggagat gggttttata gtctgtaatc
    11341 atctcaaact cgcctgcttc aaccttagga atcatgactt gatagataaa ttccttgacc
    11401 ttggccacat ccttatccaa aagaagggca ccaagaaagg cttcaaaggc atcaccaaga
    11461 atggtgtcac gattgcgacc accagatttt tcttcccctt tacccaactt gataaactgg
    11521 tcaaactggc aatcacgcgc aaaaccagct aaactctcct cacggacaat catagcacgg
    11581 agttttgata ggtcaccttc aggcttttta ggatattttt tatacagata ttctgaaatc
    11641 aataactgta gaacagcgtc tcctaaaaat tccaagcgtt cattgtgtga aatttttaag
    11701 aggcggtgct cattggcata actcgtatga gtaaaggcag tttccagtaa atttttgtct
    11761 gtaaattcga ttgcaaaatg gttctttagt acagtttgta attctttcat accaacctct
    11821 ttctaactga taacagtcct ttttattata tcaaaaaaag ccccctgagt cactctaaaa
    11881 cgggactgga aagcatttgg gaattcttta gacagagatt ctcagtttta gcggcaaatt
    11941 tagatcagtg taaagaaaaa agccctatta aaggcttttt aggatgttta catccaccct
    12001 gagggaatcg aacccccatc tcaagaaccg gaatcttacg tgatatccat tacactaagg
    12061 gtggaaactt gttttattat aacagaaatt tgctctaata acaagttttt tagtcaaagc
    12121 ccccgtctta gtgggaagca tccccattcc agatggagtt tttcacgatc acataatcaa
    12181 cgtgtttaag gtcagcaacc tgacgtccac ctgcataaga aatagcactt tgaaggtctt
    12241 gttccatctc agttaaagtg tcttgcagat gacctttagc aggaagcaag atacgtttgc
    12301 cttccacatt tttgtaagca cctttttgat attgtgaggc tgaaccataa tattctttga
    12361 actgttcacc atcgacttca atcgttttcc ctggactttc aatgtgtcct gcaaagaggg
    12421 aaccaatcat gatcatgcta gcaccgaagc ggatagactt ggcaatatca ccgtgagtac
    12481 gaattcctcc atcagcgata atcggtttac gcgcagcctt ggcacaccag cgtagagcag
    12541 ccaactgcca accacctgta ccaaaaccag tcttaacctt ggtgatacaa accttaccag
    12601 gaccgattcc gaccttagta gcatccgcac cagcattttc caattcacgc acagcttctg
    12661 gtgttcccac atttccagca atgacaaagg tatctggcaa ttctttcttg atgtgttgaa
    12721 tcatagaaat cacgctatcc gcatgaccat gagcaatatc aatagtgata tactcaggag
    12781 catcagtctt gagctgtcta acgaaatcat actcatagtc cttaacaccg acagagatag
    12841 aagcaatgag cccttgatcg tgcattcgtt taataaaagg aatgcgtcct gcctcatcaa
    12901 aacggtgcat aatgtagaag taaccacctt tagccagttg ctctgctaca ttttcatcca
    12961 aaatcgtctg catattcgct ggcacaacag gtagtttaaa ggtgtgattt cctaaagtga
    13021 cacttgtatc cgcttctgca cggcttttaa tgacacattt atttggaatc aattgaatat
    13081 cttcgtaatc aaaaattgga aattcattta acatatcgat gtctcgtttc ttttgtaatg
    13141 acctacctat gctctcgcat cactacgtct tttccgacgt ttccttaatt tattataaac
    13201 caaagtacag tttttgtcaa attattttat gaattaaaat atatcgttcg ggttttactt
    13261 gtgttaaaaa taaaagaaag aggggaaatt attttctccc cacttcttca tcaataatcg
    13321 ctttaaaatt ttcttctgaa ttttccatca ttttacaaaa tgcactatag gatgaggcat
    13381 tctgagggat tcgaatagac cacttcttca attgagctcc atagccagct acttttccta
    13441 tttcactatt tactgactgc tccatactag gaaaaatcag tccagccttc tcagttttta
    13501 aaatataaga ataggaaatc agctggaata agtcctcacg gttgattcct ttttcagtca
    13561 attccagttt tttatatttt gcatctagaa caatctttcg ttctctgtca taaaaatctg
    13621 gatatacttt tcgtttccca acaaaaaata ctgaaattcc atccgtctta tccttatttc
    13681 tgggatgtac aaaacctttt ggcaacaagg tgtaaacata ctcttcccaa agccaggcaa
    13741 catcaaagag aataccatag attttttgat cttgataccc taaaccgtgc ttttcttggt
    13801 ttaggatcat cagacaaagt tcttgtaagt ttctgtactc gtgaaagtat gcatgacgta
    13861 taggtttaga ttgatttccc cgaataatct tagcacgatc agctagttta taagagggcg
    13921 ttacacgcac gatttcagat acgttttcac gactagttga gagattatct agtacccctt
    13981 gaccaatgct tttctgattc ttaatgtatt caatagtgtg acggaccaac tgcatgaggg
    14041 gattatcata ggtgaactct ctcgttgtgt aggcaatatt tcccgtgaaa ggaagatttt
    14101 tcttgagatg gtttcttaca tcaatcactc ccttaacatg actgtcgtta tgagaaaatc
    14161 gatgatattc cttataaaga ccttttcgaa tagcggcttg tagatacttg gggaagagat
    14221 acatcaaaag ttgataaagc ctctcttcac gagacaaagc aacatctaaa ctagtgagat
    14281 tgatataaag aaccttgttt aagagataat gcaaaaaatg gtcattactc tcatcagaaa
    14341 atcgtgagga aatcgttaat ctttcctgac catatccaag aaaaccaatc acgttccctg
    14401 tcttgatttt ctgattgact gtttcaaaaa tcttttggtc cttatccaaa tcaggagtat
    14461 gagtcaaatc atttggaaaa atgaaaatac gttcatcttg ggatagactt tccaaggttc
    14521 tgtctaaaag gagattgctt agtttaggat attcttcaac aaatttttct ttaataatct
    14581 tgtgttgatt atcagtaatc cgcatcatcg ttttcatcac cttcattgtc atcagctact
    14641 gcctgatctt tttgctcatt atttgtcagt tcaaatgctt ttttcaaagt ttccaaagtt
    14701 tcaacctcat cataagaacc tcgcaagtag tcttctagga gaggcttaat ataatcagac
    14761 cagagtaatt catagtcaaa atctacatcc ttcaacttaa gaaaataact tggtccaata
    14821 tgataatgac tgtttaattc ctgaacattt tcgatagcag cgttcaagtt tcttagacga
    14881 atttttgctt cttctgcatg gatattcagc tctttatcca acatgccaac ttgaccctcg
    14941 gcagtaactt caacaaaacg aaaacgacga cgcatagcaa aatcaaaggt atccactgaa
    15001 cgatcaatat cattcatagt tccgatgatg taaacatttt cggggatata gaacttttca
    15061 tcagtttcgt gtagatttgc atattgggta gaaacacttc ctttttcacc acgatagcca
    15121 gggtcgatag agaaaaagag ttcgccaaaa atcttagaaa tctccccacg attgatttca
    15181 tcgatgatga agacgaattt cttatcagta tcaatttctg ttggggaaac atagtctttc
    15241 aaaccaaatc tctttctcaa tgtttctagg acagtttttc caccactttt gtagtattct
    15301 tgcttattat aatttttatc tttatacaac tcgtaaacat atttgctagg tagtgaccat
    15361 cctggaacac cactatcata atttactgac aaattttgtc tactattaac agataagtaa
    15421 gatgtttttg ttatatattc tttttcttca gcaacattta tatattctaa gtaagaatcc
    15481 caagcctcat caaaattatc ttgtcctcca atcaattggg tttcttttgc tttctgacaa
    15541 aaatctttaa aaataccgtc ctgtagccta aactcaatag ctccatcccc atttgatact
    15601 ggtcttaaac cttctacaaa atccgtataa tcatatgatg ggtgaaattg tacaaatccg
    15661 atttggtctt cgttgccatc cgttaattct ttggcaattt ctttagcaag ataagtcttt
    15721 cctgtgccag gagcaccacg aaggataagg tttggagact ttaatagaga ttgagttaat
    15781 tgatgacgaa aattgtttga tgaatccatt gtactattgt cttccttatt ttccataaaa
    15841 agcttagaat actcaaaaag catagttcct aatttcgatg gctccaactc aggataagtt
    15901 ttggacacca gttcaattat tttatgattt tgcagataag gtccaccttg atttgtctga
    15961 gaaacaaagt ggttccaaaa ttctttctga ctattaatat ttacaccaaa gaaaggatct
    16021 ccctcagaat agatacaaag taattttgtc accatagcag aacgaccaat a
//
LOCUS       DAVHIT010000045        15805 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVHIT010000045 DAVHIT010000000
VERSION     DAVHIT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.046"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     regulatory      57..145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            360..1028
                     /locus_tag="VVN16_001252"
     CDS             360..1028
                     /locus_tag="VVN16_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU5572639.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1271..2053
                     /gene="thiM"
                     /locus_tag="VVN16_001253"
     CDS             1271..2053
                     /gene="thiM"
                     /locus_tag="VVN16_001253"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU5572640.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            2055..2684
                     /locus_tag="VVN16_001254"
     CDS             2055..2684
                     /locus_tag="VVN16_001254"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5572641.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      2893..2981
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3243..3803
                     /locus_tag="VVN16_001255"
     CDS             3243..3803
                     /locus_tag="VVN16_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5572642.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3804..5189
                     /locus_tag="VVN16_001256"
     CDS             3804..5189
                     /locus_tag="VVN16_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572643.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLSHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5191..5841
                     /locus_tag="VVN16_001257"
     CDS             5191..5841
                     /locus_tag="VVN16_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU5572644.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5852..6544
                     /gene="tenA"
                     /locus_tag="VVN16_001258"
     CDS             5852..6544
                     /gene="tenA"
                     /locus_tag="VVN16_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU5572645.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFYISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6549..7073
                     /gene="thiW"
                     /locus_tag="VVN16_001259"
     CDS             6549..7073
                     /gene="thiW"
                     /locus_tag="VVN16_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU5572646.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            7073..7876
                     /locus_tag="VVN16_001260"
     CDS             7073..7876
                     /locus_tag="VVN16_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU5572647.1"
                     /translation="MQEFTNPFPIDSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            7869..8501
                     /locus_tag="VVN16_001261"
     CDS             7869..8501
                     /locus_tag="VVN16_001261"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5572648.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8710..9501)
                     /gene="thiD"
                     /locus_tag="VVN16_001262"
     CDS             complement(8710..9501)
                     /gene="thiD"
                     /locus_tag="VVN16_001262"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU5572649.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDSLIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(9585..9683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9867..10262
                     /locus_tag="VVN16_001263"
     CDS             9867..10262
                     /locus_tag="VVN16_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU5572650.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKFFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            10273..10644
                     /locus_tag="VVN16_001264"
     CDS             10273..10644
                     /locus_tag="VVN16_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5572651.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            10654..12897
                     /locus_tag="VVN16_001265"
     CDS             10654..12897
                     /locus_tag="VVN16_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5572652.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLVISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            13104..14879
                     /gene="spxB"
                     /locus_tag="VVN16_001266"
     CDS             13104..14879
                     /gene="spxB"
                     /locus_tag="VVN16_001266"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU5572653.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            14990..15337
                     /locus_tag="VVN16_001267"
     CDS             14990..15337
                     /locus_tag="VVN16_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5572654.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(15454..>15805)
                     /locus_tag="VVN16_001268"
     CDS             complement(15454..>15805)
                     /locus_tag="VVN16_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5572655.1"
                     /translation="AGRCNTEWMNAFLEELSQAYPYDYFLLVIDNAIWHKSSTLKIPT
                     NIGFAFIPPYTPEMNPIDQVWKEIRKRGFKNKAFRTLEDVMNQLQDVIQGLEKEVIKS
                     IVNRRWTRMLFESR"
BASE COUNT         4657 a         2900 c         3480 g         4768 t
ORIGIN      
        1 tcaaatatct tgctagggct ttcatttttt gctatactgg ttcagtaatt aaataataga
       61 gacatttggg gtgctttaag ctgagatgat acccattgaa cctgatacag ttaagactgg
      121 cgaagggaaa tgtgaaacgt tttttcgtat ggcgaaatga acgatctaat cttgtaagtc
      181 aactctaatt cttgattgta attggagttt tttgtttata taaactggat aggcttgttt
      241 agttagctct ctgcaaatgc tctcgatgtg ctttaaaagg atcaagtgga attacgattt
      301 tgcttgatat ttcttggtgt tttattaatt actaacaagt aaagagagga gaagtaaata
      361 tggaaacaca agactatgca tttcagccag gtttgactgt tggagaatta ttaaaaagca
      421 gtcagaagga ttggcaggct gcaatcaatc atcgttttgt taaggaactt tttgcgggga
      481 caattgagaa taaggtctta aaggactatc tgattcaaga ttatcacttc tttgatgcct
      541 tcttatccat gctgggtgcc tgcgtagctc acgcagataa acttgaatcc aagcttcgtt
      601 ttgccaagca actaggcttt cttgaagcag atgaagatgg ttatttccaa aaggctttta
      661 aagagttaaa agtagcagag aatgactatc tagaagtgac tttgcaccct gtaacaaaag
      721 cctttcagga tctaatgtat tcggcggtgg cttcatcaga ctatgcccat cttttggtca
      781 tgctggtcat tgcagaaggt ctctatttag actggggttc taaagatttg gctttacctg
      841 aagtctatat tcattcggaa tggatcaatc tccacagagg tcctttcttt gcagagtggg
      901 ttcaatttct ggttgacgaa ctcaatcgtg tcggtaaaaa tcgagaagat ttgacagaac
      961 ttcagcaacg ctggaatcaa gcagtcgctt tagaattagc tttttttgat attggttacg
     1021 atgtctagag aaggctcatg atatttacaa atttgatgag gtaagactct tcgaaaatct
     1081 cttcaaacca cgtcagttct atccacaacc tcaaaacagt gttttgagca acctgcggtt
     1141 agcctcctag tttgctcttt gattttcatt gagtataaat cattcaatgt aaatgaaaaa
     1201 aattcttaac gaaagataga aataaatcga aatcagtaga tcgtataaag tgaaaaggaa
     1261 ggatttcaat atgacaagtt taaaattatt aaaagaaaaa gcaccattgg tcatttgtat
     1321 aaccaatgat gtagtaaaaa atttcacagc aaatggatta gtagcactgg gcgcctcacc
     1381 agccatgagt gagtttccag cagatttaga ggatttgtta aagtatgctg gtggtttatt
     1441 aataaacata ggaacattga cagatgaaaa ttggaaatta taccaagctg ctctgaaaat
     1501 tgcggagaaa tataatgtcc cagcagtttt agatcctgta gcctgtggag caggagaata
     1561 tagaaaaaaa gtagcggatg atctaatcaa caattataaa ctagcagcga ttagaggaaa
     1621 tgctggcgag attgcctctt tagtaggaat agatgtggca tctaaaggag tagatagtgc
     1681 gggcgtagat aatattgacg aaattgctct agcagcaaat gagaagttca atattccaat
     1741 agtagtaaca ggtgaagtgg atgccattgc tgttaatgga gaagtggtaa cgattcataa
     1801 tggtagtgct atgatgccga aagtcattgg gacaggatgc ttattaggag ctgtagtagc
     1861 aagctttatc ggactagaaa aaggtcaaga attgaaatca ttagaaacag caatgttggt
     1921 ttacaatatc gctggagaaa tggcagaaaa acgtcccaat ggacatcttc ctggaacatt
     1981 taaagctgaa tttataaatg ccttatacga gattacagat gaagatgtaa aggaattcaa
     2041 aagagtgaag taagatgttt cataaagaat tactaaaact atattttatt tgtggaacga
     2101 ctacttgcca aggaaaaaat ctatatacag tcgttgagga agccttaaaa ggtggtataa
     2161 ccttatttca atttcgagaa aaaggtgagg gtgctttaga aggcttagaa aaacttgagc
     2221 tagctatcca aattaaagaa ctttgtaaaa aatacaatgt tccatttatc gttaatgacg
     2281 atatagattt agcaatggaa attgacgccg atggcgtaca tgtaggtcaa gatgatattg
     2341 gtgttgatga aattagaaaa ttgatgccag ataaaataat tggtctttct ataagaaacg
     2401 aagaagaatt tcaacaatca aaagttgaat atgtagatta tgtaggtgtt gggcctgtat
     2461 ttgataccca gtcaaaagat gatgctggtg gtgctatagg ttatgaaggt cttgaattga
     2521 tgagaaaact attgccacaa atgcctttag ttgcaattgg tgggatacag acgaaacata
     2581 ttaaagacat tataaagacc aatgttgacg gcgtttcaat catttcggca atatcgtatg
     2641 caaaaaatat agaaaaaact gttcgggaaa tgagtgaaca atagatatct atccttggat
     2701 tctaagtaag taggattttc cttttttaaa aagctatgtc tagattcagc ttgaatggct
     2761 aattcatcaa acaggtcatt tgttttttgc aaaagaaaga tctgctgtct ttgtgagtgc
     2821 aaggctgtat ttccagttca atttcttgaa ctagtataaa atgtgtgtta taataatcac
     2881 ataattaaat aataaaggca tttggggtgc tttaggctga gatgataccc attgaacctg
     2941 atacagttaa gactggcgaa gggaaatgtg aaatgttttt ttcgtatgtc gaaaagaact
     3001 gtctaatctt gtaagctgaa ctctaattcg tatctgtaat tagagttttt tcttttacga
     3061 aactggatga agatgatata gtagattgaa ataagatgcg aacaaatcaa ttagaaaatt
     3121 caaattaatt tctagaaata ttttagcatt cctgtgtact gttctaaatt caatctgcta
     3181 tacaaagtat ttaggtcggt cttcttgagt gccccacttt tcttttaaaa aaggagtttt
     3241 ttatgttgaa aaaatggcag ttaaaagatg ttatcttgct tgctttcttg tctatctttt
     3301 ttggtggggt tttcgttggt tcaggatatg tgtataatat tctcagtcta ctcttaacac
     3361 ctcttggttt gcaggccttt gccaatgaaa tcctcttcgg tctctggtgt atggctgcgc
     3421 ccattgctgc catctttgtt ccgagagtcg gaagtgcaac gattggagaa gtgctagctg
     3481 cgcttgctga agtcctttat ggtagccaat ttggtctagg agctcttttg tctggcttgg
     3541 ttcaaggttt gggaagtgaa tttggtttta tcgtaactaa gaatcgctat gaaagttggc
     3601 tctctctaac tgctaatagt attgggatta cgcttgttag ctttgtctat gaatacatta
     3661 agttaggtta ctacgccttt tcccttccgt ttgtcctttc cttacttgtg gtacgtttta
     3721 tttctgttta tttcttctgt accatcttgg ttcgtgccat tgtcaaactc tatcatcagt
     3781 ttgcaactgg aggaaaagca tagatgggtc tggaactacg agcgattcag tccccaatct
     3841 tctctgagcc gtttgatttt acttttcatg cgcaagcctt taccttgtta gttgggagca
     3901 gtggttcagg aaaatccagc ctttttcaag tcattgccca agtcagttct cttccctata
     3961 gtggtcaagt cctgatagat gggagcgagg tcagtcagct ttctatcatc gcacgtgtcc
     4021 agaaggttgg cattctcttt caaaatccca atcatcaatt taccatggag aacttgtttg
     4081 aggagctgat tttcaccttg gagaatatcg gctatcacct tcaggaaatt gattctaaaa
     4141 tagcagaggt tgtccagcaa tgtcgttgcg aggcaatctt acaccgtccc attcatcacc
     4201 tatcaggtgg ggaaaagcaa aaagcagcgc tggctgtcct ctttgccatg aatcctaggg
     4261 tctatctctt ggatgagccc ttcgcttcta ttgaccgcaa gagcagaatc gagatattgg
     4321 agattctaaa agagttggct cttgatggga agacagttat tttgtgcgac catgatttat
     4381 ctgactataa agcctatatc gaccatatgg ttgagctaag agacggaaaa ctaagggaag
     4441 tgtttcaaat cccttcctat gagatgacac aggttgcttc aaaggaagtt gcttctagcc
     4501 cggaactatt ccatatgaac cgtgtgactg gtgagcttgg taatcgcccc ctcttttcaa
     4561 ttgctgattt cacattctat caagggattt cctgtatcct gggtgacaat ggtgtcggga
     4621 aatcaaccct ctttcgctct attcttcaat ttcaaaagta taaggggcgc attgcatgga
     4681 aggggacagt cctgaaaaag aaaaagagtt tgtaccgtga tctgacgggt gttgttcagg
     4741 aagctgagaa gcagtttatc cgagtcagtc tgcgagagga gcttcaatta gatggacctg
     4801 attctgaaag aaatcagcgg atttttcaag ctttacgata ttttgatttg gagcaggcag
     4861 tcgataagag tccctatcaa ttaagtggtg gtcagcaaaa aattcttcag ctcctgacca
     4921 tcttgaccag taaggcttcc gtgatcttgc tagatgaacc ttttgcaggt ttggatgata
     4981 gagcctgcca ttatttttgc aagtggattg tggaggagag gaatcaagga agaagttttc
     5041 tgctcattag tcatcgttta gaccctttga tttctgtggt tgattattgg attgagatga
     5101 ctagtcaggg gctcagtcat gtgaaagaag tgaccattac caaaccactt acatctcaga
     5161 gtagcaatac ccaaggggag gtgagatagt atggtcaaag tagcaaccca gacaccgatt
     5221 atcagtctct tcttgctgat tttatccttg gaaacatctt tcattccttc gattgctctg
     5281 aatctttcgg tagtcgcatt ttgtattctc tttatgctct attaccgtcg atttaaaatg
     5341 ttagcttgga tgatcatact tgccatttta ccatcttttg ccaactactg ggcagttcag
     5401 ttacacggag atgcttcaca ggcagtcatg cttggaacga gggcctttgt gacagtttgt
     5461 atcggccttg tctttgtttc ctctatttca ctaaaagagc ttctcttgta cttggctcaa
     5521 aaggggctat cacgctcttg gtcctatgcc ttgattgtgg tattcaattc ctttcctctc
     5581 attcagcaag aaatcaagtc cctcaaagaa gcttgcctat tacgtggtca agaactgcat
     5641 ttttggtcgc ccttgattta cagtaaggtt ctgatgacag tctttaggtg gcgccatctt
     5701 tacctgagag ctctgtctgc tcatggatat gacgaacatg cacagttgaa gaatagctat
     5761 cggacttttt atattcctaa aaaaacaaaa ttaatctacc tgcttttctt tttattgctt
     5821 caaaccagtc tatttttata aaggagttat tatggaattt acagatattg cgatggaatt
     5881 atccaagaaa gcttggcagg cttcctttca tcatcccttt attttacaat tacaagaggg
     5941 gaatttagaa cctgccattt tccgctatta cctgattcag gatgcctact atctgaaggc
     6001 cttctcagaa atctatcatc ttttggctga taagacttca aaccaagaga tgaaaagact
     6061 cttgaaacaa aatgctcagg gtttagtgga gggtgagtta tttattcgcc aacaattttt
     6121 caaggaaatg gaaatcagcg accaggaaat ggagcaacat ccaattgctc caacctgtta
     6181 tcattacatt tctcatattt ataggcaatt tgcagaaccg aacttagcca tcgcttttgc
     6241 aagcttgctt ccttgtcctt ggttatacca tgatataggc aaatcactta atcttaaacc
     6301 atcaccaaat cctctctatc aacaatggat tgaaacttat attacggatg agttagagca
     6361 acagatcaga gaggagggag cactggtcaa tcagctctat cgagaaagtg acgagacaga
     6421 caagcaaaaa atgctagatg ccttctacat cagtgttcat atggaagcca agttttggga
     6481 aatggcctac caacaccaga catggaagag cgatttacag tctttagaaa aaggagaaga
     6541 atagaagcat gagaaagcac caattacaag ttcacaaatt aaccatttta tctatgatga
     6601 ttgcccttga tgtagtcctt acacctatct ttcgaattga gggaatggca ccgatgtcca
     6661 gtgtagtcaa tattctagcg ggaatcatga tgggacctgt ttatgccttg gccatggcta
     6721 cagtcacagc ctttatccgt atgacgactc aagggattcc gcctttagct ctcacaggag
     6781 cgacttttgg agcccttctg gcaggtctct tttataagta cggtcgaaaa tttcactatt
     6841 ctgctctagg agagattttg ggaacaggta ttattggttc tattgtttcc tatcctgtta
     6901 tggtgctctt tacaggatca gctgctaagc ttagctggtt tatctacacg cctcgatttt
     6961 tcggagcaac cttgattggt acagcgattt cctttattgc ctttcgattt ttaatcaagc
     7021 aggaattctt taaaaaagtg cagggatatt tctttagtga aaggatagac tgatgcagga
     7081 atttacaaat ccctttccta tagactctag ttccctcatt cactgtatga ccaatgagat
     7141 ttcttgtgag atgctggcaa atgggatttt ggctctggga tgcaaacctg tcatggcaga
     7201 tgatccccgt gaggttcttg attttactaa gcaaagtcag gctctcttca tcaatttggg
     7261 gcatttgtca gctgagaagg agaaagcaat ccgtatagca gcttcttatg cagctcaagt
     7321 ttgtctccca atggtagtag atgcggttgg cgtaacgact tcatccattc gtaagagctt
     7381 agttaaagac cttttagact atagacctac ggtccttaaa ggaaacatgt cagaaattcg
     7441 aagtcttgtt ggattaaagc accacggcgt tggggtcgat gcgagtgcta aagatcaaga
     7501 aacggaggat ttgcttcaag tcttgaaaga ctggtgtcag acctatcctg gtatgtcatt
     7561 cttagttact ggccccaagg acctcatcgt ttcggagaat caagttgctg tactggagaa
     7621 tggctgtact gaattagact ggataacagg gacaggagat ttggttggag ccttaacagc
     7681 cgtttttctc agccaaggaa agactggttt tgaagcttct tgcttagcag tctcttatct
     7741 caatatcgct gctgagaaaa tagttgttca aggaatggga ttggaagaat ttcgttacca
     7801 agtactcaat cagctttcgc tcctaagaag agatgaaaat tggctagata ccatcaaagg
     7861 agaggcttat gaatagagaa gcacttagac tatatctggt aaccaatcgc tatcaagatt
     7921 ccgtggaaag ctttcttgca aaagttgaga cggcctgccg ttcaggggtt accatagtcc
     7981 aattgcgaga aaaaaatctc acaaccaatc aatattatca actggcaaaa caagtcaagg
     8041 aaataacaga tgcttatcag gtacccttga taatcgatga tcggttggat gtttgtcttg
     8101 cggttgatgc tgctggtctg catattggag atgacgaact cccagtttcg gttgcccgac
     8161 aagtcttggg tcctgaaaaa atcctcggtg tcaccgctaa aacggttaaa agagctctgg
     8221 aagcggagaa atcaggtgca gattacttgg ggacaggagc catttttccg acaaccacca
     8281 aggaaaatgc acccatcacc ctgatttcaa ccttgaaaac aatttgccaa acggttgcca
     8341 ttccaatagt tgctattggc ggcttgactt cggagaatat tgatcagctt atgggcactg
     8401 gcatagctgg tgtagctgtt gtacgtgatt tgatgcaagc agaagatatt gaggcaaaaa
     8461 cgcaagcctt tttaacaaag ttgcatgaca ttctttccta attaatactc aatgaaaatc
     8521 aaagagtaaa ctagaaagct agtcgcaggt tgctcaaagt actgccttga ggttttagat
     8581 aagactgacg aagtcagctc aaaacagtgt tttgaagttg cagatggaag ctggcgtagt
     8641 ttgaagagat tttcgaagag tataaaaact ctctaattcc cttaaagtgg gaactagaga
     8701 gtttttttct taatctttaa aagttgtatg gttgactgga ccagaaccat gaccgagttg
     8761 aggggcatct tgaatagctt ttgtgataaa ggccttggcc ttatcaactg cctggtaaag
     8821 actcttgccc ttggctagtt cagcagtaat cactgcagca aaggtacatc cagtaccatg
     8881 ggtgtgacag gtttgaattc gtgggctttc ccagacaaat tgttcattct tggtaaagag
     8941 gaaatcttta gcaccacctt tgagatgtcc gcctttgata accacagact gaggaccaaa
     9001 ttcttttaaa atcaggcgac cagcacgctg catgtcttcg gggtcatgga ttgaaaaacc
     9061 aacaatctct tctgcttcag gaagatttgg cgtaataata gttgctagag gtagtaagtt
     9121 tgttttgaga tagtctctag catttgagtc aatcaaggaa tctccacttg tagcaaccat
     9181 aacaggatca aggacatagg gacaatccag ttttttaaga tagggttgga tgatttccat
     9241 gatttcagta gtagccaaca ttccagtttt tacagcctga ggtggaatat cagaaaagac
     9301 actctccaat tgggctttca acatttgagg agaaacgtgc tcgattagct gaacacctct
     9361 ggtattttga gcgacaagac tggttacaac agccattcca tagacatctc tcgcttggaa
     9421 tgactttaaa tctgccataa tgccagcacc accactaggg tcagtccctg caatggtcaa
     9481 agcaacgggt aaataagtca tctaaaacct cccaaccaac tgaagtttgt attcttaaga
     9541 acaagctagt ctgttttaga aaggcaatag aagactgcta gtccccatta tcatttccac
     9601 ttccatcagc tagcattacc tagattgagt cagagggatc taacagtcgt taatctcagc
     9661 tttacgcacc cctagtggtt gttttctttt ttctttagta tagcatattt ttattgttta
     9721 tatagtaata tttgactgca aatcgttttt caaatgatta aaattctatt tttcgaagaa
     9781 aatattattt ctataattga caaatgtaga ttttgggagt atactgttga gtgtaattga
     9841 caaatgtaga ttttggaggt atggtaatgc agatttcaga tgcagaatgg caggtcatga
     9901 agattatttg gatgcagggg gagcagacca gtacagattt aattagggtt ttggcagagc
     9961 ggtttgactg gtccaagtcc acggttcaga cacttttggc tcgtctagtt gagaaagagt
    10021 gtttgactcg gaaaaaagaa ggcaagttct ttgtttattc agcgctttta actctagacc
    10081 aaagtcggga tttacttgtc caagatatca aagacaaggt ttgttcccgt aggattagga
    10141 atttgttggc tgacttgatt gtagaatgtg aatttactca gactgacttg gaagacttgg
    10201 aagctgtgat ttcagagaag aaatcaagtg ctgtaacaga agtaagatgt aattgtatgt
    10261 aaaggagacg ccatgttaaa tagtattgta accattattt gtattgccct tatcgcgttt
    10321 atcttgtttt ggtttttcaa aaagcctgaa aaatctggac aaaaagccca gcaaaaaaac
    10381 ggataccaag agattcgagt ggaagtcatg ggaggctata ctcctgagtt gattgtcctc
    10441 aagaaatcag tgccagcccg cattgtcttt gaccgcaagg atccttcacc atgtctggat
    10501 caaattgttt ttccagattt tggtgtacat gcgaacctgc caatggggga agagtatgta
    10561 gtggaaatca cgcctgaaca ggctggagag tttggctttg cttgtggtat gaacatgatg
    10621 cacggcaaga tgattgtaga gtaggtggag actatgacag aaattgtgaa agcaagctta
    10681 gaaaatggca ttcaaaaaat ccgtatccga gctgaaaaag gctatcatcc agcccatatc
    10741 cagcttcaaa agggaattcc agctgagatt acctttcatc gtgctactcc ttcaaactgt
    10801 tataaggaaa ttctgtttga agaagaaggt atcttggaac caatcggcgt agatgaggag
    10861 aaagtcattc gttttacgcc tcaagaatta gggcgacatg aattttcttg tggcatgaag
    10921 atgcaaaagg gaagctatac agtcgttgag aagactcgaa aatctctatc tctcctgcaa
    10981 cgtttttgga ttactagtat ctttactgtg cctcttgtga ttctcatgat tgggatgttg
    11041 acaggtagca ttagtcatca agtcatgcat tggggaacct ttttagcaac aacgcctatt
    11101 atgttagttg caggtaagcc atatatccag agtgcttggg ccagttttaa aaagcacaat
    11161 gccaacatgg ataccttggt tgcgctggga actctagtgg cttatttcta tagcctagtt
    11221 gctctctttg ctggtctccc tgtttacttc gaaagtgctg gatttatcct ctttttcgtt
    11281 cttttgggag cagtttttga ggaaaaaatg agaaaaaata cgtcccaagc tgtggagaaa
    11341 ttactggact tgcaagctaa aaccgcagaa gtcttgagtg atgatagtta tgtccaagtt
    11401 cctttggaac aagtcaaggt aggcgacctg attcgagtgc gtcccggtga aaagattgct
    11461 gttgatggtg tcgtagtaga aggtgtctct agtattgacg aatccatggt gacaggtgag
    11521 agtctgcctg tggacaagac agttggagat actgtcattg gctcaaccat caatcatagt
    11581 ggaacgcttg tctttagagc agaaaaagtt ggctcagaga ctgttttggc tcagattgtg
    11641 gattttgtga agaaagctca gacaagtcgt gcgccgattc aggacttgac ggataagatt
    11701 tcagggattt ttgtcccagt agttgtcatt ttaggaatca tgaccttttg ggtttggttc
    11761 gtcttgctca gggatagtgt ggttgtgctt ggagctagct ttgtgtcctc tcttctctac
    11821 ggagtggcgg ttttgattat cgcctgtcct tgtgccttgg gacttgcaac accgacagcc
    11881 cttatggtgg ggacaggacg tagtgccaag atgggggttc tcctcaaaaa tggaactgtc
    11941 ttacaggaaa tccagaaagt tcaaactctt gtctttgata agaccgggac tttgacggaa
    12001 gggaaacctg tggtaacaga tgtcatcggc gacgaagtag aagtgtttgg gttggcagcc
    12061 tccttggaag atgcttctca acacccactg gctgaggcta tcgttaagcg agcgagtgaa
    12121 gctggacttg agtttcaaac tgttgaaaat tttcagacct tgcacgggaa aggtgtttca
    12181 gggcgaatca atggaaaaca agttttactt ggaaatgcta aaatgctgga tggcatggat
    12241 atttctaata cttatcaaga taaactagaa gaactagaaa aagaagctaa gacagttgtg
    12301 ttcttagctg ttgacaatga aatcaaaggc ttgcttgctt tgcaagatat tcctaaggaa
    12361 aatgctaagc tagtcatcag tcagctaaaa aaacgtggtc tccgaacagt catgctgaca
    12421 ggagacaatg ctggtgtggc gcgtgctatt gcagatcaaa tcggaattga agaggtcatt
    12481 gcaggcgtct tgccagaaga aaaagcccat gaaatccata aactgcaaca gtcagggaaa
    12541 gtagcctttg ttggggacgg tatcaatgac gctcctgccc ttagtgtagc agatgtgggg
    12601 attgctatgg gagctggaac agatatcgcc atcgaatcag cagatttggt gttgacaacc
    12661 aataatcttt taggagtggt tcgtgccttt gatatgagta agaaaacctt tcatcgaatt
    12721 ctactcaatc ttttctgggc ttttatctac aatgttgttg gaattccgat tgcagcagga
    12781 gtcttttcag gtgttggact ggctctcaac ccagaattgg caggtctagc catggccttt
    12841 agttctgtat ctgttctgac cagttccctt ctgttaaatt ttagtaaaat agactaaaag
    12901 taatgactag atttctttgt tataaaacag aaacgacaac actttcaaaa ctgtattata
    12961 atagagaata gagagttttg agcagatttt tagaaaagtc agcataaata tgatacagtg
    13021 gaatagtaaa aatttggaga acgtttccaa ttctatgtaa tcgtattctc caagtctaaa
    13081 aaaattgaag gagagttatc attatgactc aagggaaaat tactgcatct gcagcaatgc
    13141 ttaacgtatt gaaaacatgg ggcgtagata caatctacgg tatcccatca ggaacactca
    13201 gctcattgat ggatgctttg gctgaagaca aagatatccg tttcttacaa gttcgccacg
    13261 aagagacagg tgctcttgca gcggttatgc aagctaaatt cggcggctca atcggggttg
    13321 cagttggttc aggtggtcca ggtgcgactc acttgattaa cggtgtttac gatgcagcta
    13381 tggataacac tccattccta gcgatccttg gatcacgtcc agttaacgaa ttgaacatgg
    13441 atgctttcca agagcttaac caaaacccaa tgtacaacgg tatcgctgtt tacaacaaac
    13501 gtgtagctta cgctgagcaa ttgccaaaag taattgacga agcctgccgt gctgcagttt
    13561 ctaaaaaagg tccagctgtt gttgaaattc cagtaaactt cggtttccaa gaaatcgatg
    13621 aaaactcata ctacggttca ggttcatacg aacgctcatt catcgctcct gctttgaacg
    13681 aagttgaaat cgacaaagct gttgaaatct tgaacaatgc tgaacgccca gttatctatg
    13741 ctggatttgg tggtgttaaa gctggtgaag tgattactga attgtcacgt aaaatcaaag
    13801 caccaatcat cacaactggt aaaaactttg aagctttcga atggaactat gaaggtttga
    13861 caggttctgc ttaccgtgtt ggttggaaac cagccaacga agtggtcttt gaagcagaca
    13921 cagttctttt ccttggttca aacttcccat ttgctgaagt ttacgaagca ttcaagaaca
    13981 ctgaaaaatt catccaagtc gatatcgacc cttacaaact tggtaaacgt catgcccttg
    14041 acgcttcaat ccttggtgat gctggtcaag cagctaaagc tatccttgac aaagtaaacc
    14101 cagttgaatc aactccatgg tggcgtgcaa acgttaagaa caaccaaaac tggcgtgatt
    14161 acatgaacaa actcgaaggt aaaactgagg gtgaattgca attgtatcaa gtttacaatg
    14221 caatcaacaa acatgctgat caagacgcta tctactcaat cgacgtaggt aacactactc
    14281 aaacatctac tcgtcacctt cacatgacac ctaagaacat gtggcgtaca tctccactct
    14341 ttgcgacaat gggtattgcc cttcctggtg gtatcgctgc taagaaagac aatccagatc
    14401 gccaagtatg gaacatcatg ggtgacggag cattcaacat gtgctaccca gacgttatca
    14461 caaacgttca atacgacctt ccagttatca accttgtctt ctcaaatgct gagtacggct
    14521 tcatcaagaa caaatacgaa gatacaaaca aacacttgtt tggtgtagac ttcacaaacg
    14581 ctgactacgc taaaattgcg gaagctcaag gagctgttgg attcacagtt gaccgtatcg
    14641 aagacatcga tgcagttgtt gcagaagctg ttaaattgaa caaagaaggt aaaactgttg
    14701 tcatcgatgc tcgcatcact caacaccgtc cacttccagt agaagtactt gaattggatc
    14761 caaaacttca ctcagaagaa gctatcaaag ccttcaagga aaaatacgaa gcagaagaac
    14821 tcgtaccatt ccgtctcttc ttggaagaag aaggattgca atcacgcgca attaaataat
    14881 tcctctcgcc gaaaatcaaa tatgaaactt gtagacttta ttctttgatt ttcatagagc
    14941 agaactttaa aagatcggag caatccggtc tttttatgga ggagagtaaa tgaatttaaa
    15001 tcaattagat attatcgttt caaatgttcc ccaagtctgt gctgacttgg agcatatctt
    15061 ggataaaaag gcagattatg ctgatgatgg ttttgctcag tttacgattg gtagtcactg
    15121 ccttatgttg tcacaaaatc atttggttcc tttggaaaac tttcagtcag gaatcattat
    15181 tcatatcgag gttgaggatg tagaccagaa ctacaaacgg ttgaacgagc ttggtataaa
    15241 ggttttacac ggaccaactg taaccgattg gggaacagag tccttattag ttcaaggacc
    15301 tgctggtcta gtgcttgatt tttatcgtat gaaataggtc gcgtagtcat ttgacgttaa
    15361 ccttagctat ttttaaaata gaaattgaga atatgaacta ttgaaagatc ggagcaatcc
    15421 ggtcttttta tataaagaaa ttgcatataa tactcatctg ctttcaaaaa gcattctagt
    15481 ccatctccga ttaacgatgg actttatcac ctccttctcc aatccttgta tgacatcttg
    15541 gagttgattc atgacatctt ccaaagttcg aaaggcttta ttcttaaatc cacgtttacg
    15601 aatctctttc cacacttggt caatggggtt catctctggt gtgtatggag gaataaatgc
    15661 aaagccaata ttagtcggaa tctttaaggt acttgattta tgccatatag cattgtctat
    15721 aacgagtaaa aaataatcat atggataagc ttgtgaaagc tcttctaaaa aggcgttcat
    15781 ccactcagta ttacatctac cagct
//
LOCUS       DAVHIT010000046        15564 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVHIT010000046 DAVHIT010000000
VERSION     DAVHIT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.047"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            181..1968
                     /locus_tag="VVN16_001269"
     CDS             181..1968
                     /locus_tag="VVN16_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU5572656.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1965..2441
                     /locus_tag="VVN16_001270"
     CDS             1965..2441
                     /locus_tag="VVN16_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU5572657.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2469..3374
                     /locus_tag="VVN16_001271"
     CDS             2469..3374
                     /locus_tag="VVN16_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU5572658.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWIADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3361..4176
                     /locus_tag="VVN16_001272"
     CDS             3361..4176
                     /locus_tag="VVN16_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU5572659.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4183..4587
                     /locus_tag="VVN16_001273"
     CDS             4183..4587
                     /locus_tag="VVN16_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5572660.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4840..6006
                     /locus_tag="VVN16_001274"
     CDS             4840..6006
                     /locus_tag="VVN16_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5572661.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6122..7159
                     /locus_tag="VVN16_001275"
     CDS             6122..7159
                     /locus_tag="VVN16_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU5572662.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7367..8221
                     /locus_tag="VVN16_001276"
     CDS             7367..8221
                     /locus_tag="VVN16_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5572663.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAG"
     gene            8371..8628
                     /locus_tag="VVN16_001277"
     CDS             8371..8628
                     /locus_tag="VVN16_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU5572664.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8737..9033)
                     /locus_tag="VVN16_001278"
     CDS             complement(8737..9033)
                     /locus_tag="VVN16_001278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572665.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSPHNKTHTIKVFMYCPPKVRQKKSNFWGAVHFNT"
     gene            complement(9087..9370)
                     /locus_tag="VVN16_001279"
                     /pseudo
     CDS             complement(9087..9370)
                     /locus_tag="VVN16_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(9360..9521)
                     /locus_tag="VVN16_001280"
     CDS             complement(9360..9521)
                     /locus_tag="VVN16_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU5572666.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            9591..10355
                     /locus_tag="VVN16_001281"
     CDS             9591..10355
                     /locus_tag="VVN16_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU5572667.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(10595..10747)
                     /locus_tag="VVN16_001282"
     CDS             complement(10595..10747)
                     /locus_tag="VVN16_001282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572668.1"
                     /translation="MKTKASLRKLVTGCSKHCFEVADRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            10965..12344
                     /locus_tag="VVN16_001283"
     CDS             10965..12344
                     /locus_tag="VVN16_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU5572669.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12358..13023
                     /locus_tag="VVN16_001284"
     CDS             12358..13023
                     /locus_tag="VVN16_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5572670.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQFWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13158..13316
                     /locus_tag="VVN16_001285"
     CDS             13158..13316
                     /locus_tag="VVN16_001285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572671.1"
                     /translation="MKIKDQTRKLATGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            13378..13551
                     /locus_tag="VVN16_001286"
     CDS             13378..13551
                     /locus_tag="VVN16_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU5572672.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGWDQVGNFSKGQYIMMHS
                     LIYRTDLLRASQF"
     gene            13618..14028
                     /locus_tag="VVN16_001287"
     CDS             13618..14028
                     /locus_tag="VVN16_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU5572673.1"
                     /translation="MYYLPVDFYRYLIGREGQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14144..>15564
                     /gene="pavB"
                     /locus_tag="VVN16_001288"
     CDS             14144..>15564
                     /gene="pavB"
                     /locus_tag="VVN16_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU5572674.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISK
                     ETVQKAVADNVKDSIDVPAAYLE"
BASE COUNT         4382 a         2961 c         3533 g         4688 t
ORIGIN      
        1 tccgaattta aagagcgttt gaaatgggtc taaaaatgtt tgaaactaat tgaatgtgtg
       61 tataatagta aaaatgcagt aaatatcccg acaatttcta ttgacaattc aaacagattg
      121 gtttataatt aatataacaa caaatgaaag cgcaaacttt cgcggtcgga aggtagtttt
      181 atgacacgat ttgagatacg agatgatttc tatctcgatg gaaaatcatt taagatttta
      241 tctggtgcca ttcattattt tagggttcct ccagaggatt ggtatcattc gctctataac
      301 ttgaaggctc ttggttttaa tacggtagag acttatgttg cttggaattt acacgagcct
      361 cgtgaaggtg agtttcattt tgacggtgat ctggatttag agaaatttct ccaaatagcg
      421 caggatttgg gtctctacgc aattgtgcgt ccgtctccat ttatctgtgc ggaatgggaa
      481 ttcggtggct taccagcttg gctcttgact aagaacatgc gaattcgctc atccgaccca
      541 gcatatatcg aggcagttgg tcgctactat gatcagttat tgccaagact ggtgcctcgt
      601 ttgttggaca atggtggcaa tattctcatg atgcaggttg aaaatgagta tggttcttac
      661 ggagaagata aggcttacct gagagcgatt cgacagctaa tggaagagtg tggcgtaacc
      721 tgtcccctct ttacatcaga tggtccatgg cgagctactc tgaaagctgg aaccttaatt
      781 gaagaggacc tctttgtaac aggaaacttt ggttctaagg caccttacaa cttttcgcag
      841 atgcaggaat tctttgatga acatggtaag aaatggccac tcatgtgtat ggagttctgg
      901 gatggttggt tcaatcgctg gaaagaaccg attatcacac gggatcctaa ggaattggca
      961 gatgcagttc gagaggtttt ggaacaaggc tctatcaatc tttacatgtt ccacggtggt
     1021 acaaactttg gtttcatgaa tggttgctca gctcgaggaa ctttggacct gccacaagtt
     1081 acgtcttatg attacgatgc ccttctggat gaagaaggaa atccaactgc taaatatctt
     1141 gcagtcaaga agatgatggc aacacatttt tcagagtatc cgcagttgga accactctac
     1201 aaagagagta tggagttgga tgctattcca ctagttgaaa aagtttcttt gtttgaaacc
     1261 ttagatagct tgtcaagtcc tgtagaaagt ctctatcctc aaaagatgga ggagctggga
     1321 caaagttatg gctacctact ttatcgaaca gaaacaaact gggatgcaga agaagaaaga
     1381 cttcgtatca ttgatggtcg agatagggcc cagctgtatg tcgatggtca gtgggttaaa
     1441 actcaatatc agacagagat tggggaagat attttttatc aaggtaaaaa gaaagggcta
     1501 tctaggttag atatcttgat agaaaatatg gggcgtgtca actatgggca taagttctta
     1561 gcggatacgc aacgtaaggg aattcggaca ggggtctgta aggatctgca tttcttacta
     1621 aactggaaac actatccact cccactagac aatcctgaga aaattgattt ttcaaaagga
     1681 tggactcaag gacaaccagc cttttacgct tatgacttta cagtcgaaga gccaaaagat
     1741 acttacctag acttgtctga gtttggtaag ggggttgcct ttgtcaatgg gcagaatcta
     1801 ggacgttttt ggaacgttgg cccaactctc tcactttata tccctcatag ctatctcaag
     1861 gaaggtgcca accgcatcat tatctttgaa acagaaggtc aatataaaga agagattcat
     1921 ttaactcgta aacctacact aaaacatata aagggggaaa acttatgaca attgtaggat
     1981 gccgtattga tggacgtttg atccacggac aagtagccaa tctttgggct ggaaaactaa
     2041 atgtttcacg cattatggtt gtagacgacg aagttgtcaa caacgatatt gaaaagagtg
     2101 gtttgaaact tgcgacacca ccaggtgtga aattgagtat tttgccagtt gagaaagctg
     2161 cagccaatat tcttgctggt aaatacgata gccaacgtct ctttatcgtg gctcgtaaac
     2221 cagaccgctt ccttggtttg gtagaagcag gtgtaccact tgaaaccctt aatgttggga
     2281 atatgtctca aacaccagaa actcgttcta ttacacgttc tatcaacgta gtagacaagg
     2341 atgtggaaga cttccacaaa ctggcagaaa aaggtgttaa acttactgct cagatggttc
     2401 caaatgatcc aatttcagac tttttgagct tattaaaata ggaaaaaaat ttttaggagg
     2461 tcattgttat gatacaatgg tggcaaattt tacttctcac tttgtactca gcttatcaaa
     2521 tctgtgatga gttgacgatc gtttcatctg caggttcccc tgtatttgct ggtttcatta
     2581 ctggtttaat catgggagat gtgactactg gtttacttat cggtggtaac ttgcaactgt
     2641 tcgttcttgg ggttggtacc ttcggtggtg cttctcgtat cgacgcaact tctggtgcgg
     2701 ttcttgcgac agccttctct gtttcacaag gaattgatgc accgcttgcc attactacaa
     2761 tcgctgtacc agtagcagct ctcttgactt acttcgacgt tcttggtcgt atgactacta
     2821 ccttcttcgc tcaccgtgtg gatgctgcaa tcgaacgctt tgactataaa ggtattgaac
     2881 gcaactactt gcttggtgcg attccgtggg ctctatctcg tgcccttcca gtcttctttg
     2941 cccttgcttt tggtggtgcc tttgtacaat cagtagtaga cttcgttgaa gcctacaaat
     3001 ggattgcaga tggcttgaca cttgcaggac gtatgcttcc aggtcttgga tttgcaatct
     3061 tgcttcgtta ccttccagtt aaacgtaacc ttcactacct tgctatggga tttggtttga
     3121 cagctatgtt gactgttctt tactcatatg taacaggtct tggtggcgct gttgctggta
     3181 tcgtaggtac tcttcctgct gaagttgctg aaaaaattgg tttcgtgaac aacttcaaag
     3241 gtttgtctat gattggtatt tctatcgtag gtattttcct tgcagtgctt cacttcaaaa
     3301 atagccaaaa agtagctgta gcagcacctt ctacaccatc agaaagtggg gaaatcgaag
     3361 atgacgaatt ctaattacaa acttacaaaa gaagatttta atcaaatcaa caaacgtagc
     3421 ttgtttactt tccaattagg ttggaactac gaacgtatgc aagcttctgg ttacctttac
     3481 atgatcttgc ctcagttgcg taaaatgtat ggtgatggaa ctcctgaatt gaaagaaatg
     3541 atgaaagttc atactcaatt cttcaatact tcaccattct tccataccat tatcgctggt
     3601 tttgaccttg ccatggaaga aaaagatggt gtaggttcaa aagacgccgt taacggtatc
     3661 aagacaggtt tgatgggacc attcgctcct cttggggata caatctttgg ttcacttgta
     3721 cctgctatca tggggtcagt cgcagcaact atggctatcg ctggccaacc ttgggggatc
     3781 ttcctttgga ttgcagttgc agtagcgtat gacatcttcc gttggaaaca gttggaattt
     3841 gcttacaaag aaggggttaa ccttatcaac aacatgcaaa gtaccttgac agctttgatt
     3901 gacgctgcat ctgtacttgg tgtcttcatg atgggtgctc ttgtagcaac agtgattaac
     3961 tttgaaattt cttacaagtt gccaatcggt gaaaagatga ttgatttcca agacatcttg
     4021 aaccaaatct tcccacgttt gcttccagca atctttactg cctttatctt ctggttgctt
     4081 ggtaaaaaag gtatgaactc tactaaagct atcggtatta ttatcgtact tgctttggct
     4141 ctttctgccc ttggtcactt tgcacttgga atgtaattcc ttatgactaa atcattaatt
     4201 ttggtgagcc atggtcgctt ctgtgaggag cttagaggta gcacagaaat gattatgggc
     4261 ccacaagaca acatttacac agtagctctt cttccagaag atggcccaga agaatttact
     4321 gctaaatttg aagctgttat tgaagggttg gatgatttcc tagtctttgc ggatcttctc
     4381 ggtgggacac cttgtaatgt ggtgagtcgc ttgatcatgg aaggtcgtga tattgacctt
     4441 tacgcaggga tgaatcttcc aatggtgatt gaatttatca atgcgagcct tacaggcgca
     4501 gatgcggact acaagagccg tgctgcagaa agcattgtga aagttaatga cctgttagcg
     4561 ggcttcgatg atgacgaaga tgaataatac tcttcgaaaa tctcttcaaa ctacgtcaac
     4621 gtcgccttac cgtaggtata tgttactgac ttcgtcagtc ttatccggca acctcaaagc
     4681 agtgctttga gcagcctgcg gctagtttcc tacagatttt agttggaact cgattcaatt
     4741 catgtgacaa cgtgaaaatc gttagagcat tttatataga atatacatgg gaatgtagct
     4801 tactctcatt cccatattta atagaaaaag aggaactcaa tgctacatta tacaaaagaa
     4861 gacttgctcg aattgggtgc agaaatcact acgcgtgaaa tctaccaaca gcctgatgta
     4921 tggagagaag cttttgaatt ttatcaagca aaacgtgaag aaattgcagc cttcctacaa
     4981 gaaatcgctg ataaacatga ctatattaag gttatcttga caggtgctgg gacttctgct
     5041 tatgtgggag ataccttgct accttatttt aaggaagtct atgacgaacg caaatggaat
     5101 ttcaatgcta ttgcgacaac agatatcgtt gccaatccag caacctattt gaaaaaagat
     5161 gtggcaactg tccttgtgtc ttttgctcgt agtgggaact cgcctgaaag tgtggcgact
     5221 gttgatttgg ccaaatcctt ggtggatgag ctttatcaag tgacgattac ttgtgcagca
     5281 gatggtaaat tggctcttca agctcacggt gatgaccgta atctcttgct cttgcaacca
     5341 gctgtctcta atgatgctgg atttgccatg acttctagct ttacgtctat gatgttgaca
     5401 gctctcttgg tctttgatcc tacagaattt gctgttaagt ctgaacgttt tgaagttgta
     5461 tctagtcttg cccgtaaagt tttagacaag gcagaagatg tcaaagagct cgttgattta
     5521 gactttaacc gtgtcatcta tctaggcgct ggtcctttct ttggacttgc tcatgaagct
     5581 cagctcaaga ttttggaatt aactgctggt caagttgcga ccatgtatga aagcccagtt
     5641 ggcttccgtc acggtccaaa atctcttatc aacgacaata cagttgtttt ggtctttggt
     5701 acaacgacag actacactcg taagtacgac ttggacttgg ttcgtgaagt tgctggtgac
     5761 cagattgctc gtcgtgttgt gcttttgagt gatcaagctt ttggtcttga aaatgtcaaa
     5821 gaagtggccc ttggttgtgg cggtgtcttg aatgatattt accgtgtctt cccttacatc
     5881 gtttatgccc aactctttgc cctattgact tcactcaagg tagaaaataa accagataca
     5941 ccgtctccta caggtacagt aaaccgtgta gtacaaggtg tcataattca cgaatatcaa
     6001 aagtaagaca gtgtttatga attcttgaca agaggatttg taaattatca gataaaccat
     6061 agattgtcag tacgctttct atggtttgtt tgcttgagag aaatagtaaa aggagaacag
     6121 aatgaaagca tacacagagc gtgtatttgg aaatgttgag ggcgaggatg tcttggccta
     6181 tcgatttgag acagacggtg gctaccaact tgaggttatg acttatggcg cgaccatctt
     6241 gcgctatgtc gcacctgaca aggctggaaa ttttgccaat gttatcttgg gatttgatga
     6301 ctttgatagt tatgtaggca atagtcccaa gcatggagca agtgtaggtc ctgtagcggg
     6361 tcgtattgca ggtgcgactt ttgagctcaa tggtaagacc tatgaccttg aggttaataa
     6421 tgctagcaac tgtaatcaca gtggttcaac tggttgggat tccagcttgt ttgaagttga
     6481 agaagtaagc gatcatggct tgactctcta cacagagcgt acagatggga caggagggtt
     6541 ccctggaaat ctcaagattt ggatcagtta tcacttggaa gaaactggtg cctatgaaat
     6601 cagctacaag gtaacgaccg atcaggatac gctggtcaat ccaaccaacc acagctattt
     6661 caacttgtct ggtgatttca cgcagacgat tgaccgtcat gtcttccaac taaacacaga
     6721 gggcatttac tcaatcgctc ctgacggtgt tcctgccaaa actccagaag ccaaccgtga
     6781 tgtggtcaaa cacatctaca atggtacctt gttgaaggat atctttgcag aagaagatga
     6841 gcaaatccag ctggcatcag gtttggatca cccatttgcc cttcctgcag gccatgacaa
     6901 tgctggattc ctttatgacc aaaattcagg tcgcttcctg cttttcaaga cagaagctcc
     6961 ttgctttgtg gtctacacag caaactttgt ggatgaaagt gtcatcatag gaggtcagcc
     7021 aatgctacag cacaatggga ttgctcttga agcgcaagct ttaccagatg ccattcacag
     7081 tgaccttaaa ggccaagtca ttcttaaagc tggtcaaacc ttcaccagta agacacgtta
     7141 tgaacttgtt gtgaaataaa aaaagctggt caaaaagtct ttaaaattaa aataatgcat
     7201 attgtcaggt gatgaaaagt tcgataatat gcgttttatt atgaaatgat ttactttata
     7261 ttatcctgaa atggtgttat ttttgagtta tctggcttga ctttcttgat ggaaattata
     7321 taatagttgc aacaataata attacaacga gaaaagagga ccatttatga cctatgaata
     7381 caagagtcac atttatttgg cagagacagt tttaaatgta aaggatttgg ccagtcaaac
     7441 agccttttat cagcaagtta ttggtttaga aatcttatct caaactgaga cagagtccat
     7501 tctaggcctt ggtggaaaag tcttggtaca gctgattcaa gcacaagaga gcggagaagt
     7561 gagggaacat tatggtcttt accatttggc cattctcttg cccacacgca aggctttggc
     7621 tgatgtcttg aaacacctga cagatttaca aattcctctt gtcggcggtg cagaccatgg
     7681 ttacagtgaa gctatttact tggaagattt ggagggaaat ggcattgaac tctatcgaga
     7741 taagccagtt tccacatggg atattcgaga agatggacgc attatcggag tgacggaggc
     7801 tcttgcggcg caggacattt atgagttggg ggaaagagta gagcccttta tcctggcaga
     7861 aggtacgaga atggggcata ttcatctttc cgtcaaggat agtcgaaagt ccagccagtt
     7921 ttatcaaaag gtgttagggc tagaggataa attcagtgtg cctagcgcta gttggattgc
     7981 ggctggagat taccatcatc atttagcagt taacgaatgg ggaggaaaag gtctggcttc
     8041 gcgtaagcaa ggcttaccag gtttagccta ctatgtcatc gaagtcgcac ataaagaaga
     8101 actgttaacg attgcccaac gagcacaaga agttgacgca ccaatcaaat ggatgacatc
     8161 gagccaattg gaaatcacag actcagatgg catcgtgacc cgtattcgtt tagcagggta
     8221 aaattaggaa tttctaataa gttataagta ttgttagttt gctttttgaa cggtctttgg
     8281 taacagaact gcgtgagctg gatgggcgag aattttgaca aagaagctga ttagagatat
     8341 aatgaagaaa aatcgagcaa ggaaaaagaa atgacaaatt caaagtatat tacttgtttg
     8401 aaacgttcag agggccagtt gtgtgggatt caaaagatga ttgaaggaga tcgtgactgt
     8461 gctgatattg tgacgcaact gacagcagtg agatctagtg tagagcgcgt gattgagatg
     8521 ataattaccg aaaatcttac tgaatgtatc aatcagccac tagatgattc tgaagctcaa
     8581 aaggaacgcc tagaaaaggc tatccgatac ttgattaaac ggaaataaat gaatgttttg
     8641 agacgatatg tgcaaaggtc tattaactat tagaatagtt taatgtgagt caaaagaagt
     8701 tgtaaaaaat ctttaaaatt aaaaaaacgc atagtattag gtattgaaat gtactgcccc
     8761 ccaaaagtta gattttttct gtctaacttt tggggggcag tacataaaaa ccttgatagt
     8821 atgtgtttta ttgtggggag atttatactc ttcgaaaatc tcttcaaacc acgtcagctt
     8881 caccttgccg tagatatggt tactgacttc gtcagttcta tccacaacct caaaacagtg
     8941 ttttgagctg acttcgtcag ttccatccac aaccttaaaa cagtgttttg agcagcctgc
     9001 ggctaacttc ctagtttgca ctttgatttt cattgagtat tacttcatct tttcctgaaa
     9061 ttgagttttt gcccagctcc tcttgattat tctacttgac cgggccaagc attcatacca
     9121 ccttctacgt tggtaacggt gaggtcttgg gagctgagaa attggcaggt agatgcagag
     9181 cggactcctc cgtgacaaat gacatagtat tcatggtcag gtttgagttc tttgtagcct
     9241 tgctctaagg tatttatgga agatttttgg gtcctgcttg gaattcatgt gcttcacgga
     9301 catcgataag ttctagattt tcattttggt attttccata aaagtcagcc atgctgatac
     9361 tagtttccat attctactcc ttctgggtta accattttgt atagtgaata agcgccgtca
     9421 aggttttggg cggtaaatcc tgcttgcttg aggataggct ctgcgatata gctgcgcaaa
     9481 ccactgtggc agctgacgat gtaggcttgt tttttgtcca gttcatcagc ttcctccttg
     9541 acactttgtc agtttttgat acaatagtac aaaattagag gaggcaggct atgattcaga
     9601 aacatgcgat tcctatttta gagtttgatg acaatcctca ggcggttatc atgcccaatc
     9661 acgaggggct ggacttgcag ttgccaaaga agtgtgttta tgcattttta ggtgaggaga
     9721 ttgaccgcta tgcaagggaa gtaggggcgg attgtgtcgg cgaattcgtt tctgctacca
     9781 agacctatcc agtctatgtc atcaactaca agggtgagga ggtctgtctg gctcaggctc
     9841 ctgttggctc cgctccagca gcccagttta tggattggtt gattggctat ggtgtggagc
     9901 agattatctc tactgggacc tgtggtgtcc tagctgatat agaggaaaat gcctttctag
     9961 tccctgttcg cgctttgcga gatgagggag ccagttacca ctatgtggca ccttgtcgtt
    10021 atatggaaat gcagccagag gctattgctg ctattgagga agttttggaa gacagaggga
    10081 ttccttatga agaagtcatg acctggacga cagacggttt ttaccgagaa acggctgaaa
    10141 aggtggctta tcgtaaggaa gaaggctgtg ctgttgtgga gatggagtgt tctgctcttg
    10201 cggcagtagc tcaattgcgt ggggttctct ggggtgaatt gttgttcaca gcagattctc
    10261 tagcggactt ggaccagtac gacagtcgtg actggggctc ggaagctttt aataaggcgc
    10321 tagaactgag tttagcaagt gttcaccacc tttagttgta ctggcaaagg atttgtttta
    10381 tcataaaatg tctagctcat acttttcaaa aatatgttta aacgaggtca ccttcctctt
    10441 gtcctaggca tgttgaggtt gggaaaaatc tttaaaatca gaaaaacgta tcatatcagg
    10501 tgatgaaaac tttgatacta tgcgttttat gttggtaaga tttagagtga gatgaaatga
    10561 tactcttcga aaatcttttc aaaccaggtc agcttcacct tgccgtatag atgttactga
    10621 cttcgtcagt tctatctgca acctcaaaac agtgttttga gctgacttcg tcagttctat
    10681 ctgcaacctc aaaacagtgt tttgagcaac ctgtgactag ctttctaagc gatgccttgg
    10741 ttttcattgc ctataatcaa aaagagaaat tttctcctga aaagcatata gagtagctgg
    10801 cgttaaaagc tcctgtcttg cttttttgac ctatagtcac atctatcaag tattgttctt
    10861 gcctaagcta tcaataaaaa ggtggcattt tttaggcttg gtgttagtga attttgcctt
    10921 atcctatcta agtcatttcg agctttttat ggtacaatgg aaacatgtta ttcaaattat
    10981 ctaaggaaaa aatagagcta ggcttatctc gtttatcgcc agcccgtcgt atttttttga
    11041 gttttgcctt ggtcatttta ctaggctctc ttcttttgag cttgcccttt gtccaagttg
    11101 aaagctcacg agcgacttat tttgatcatc ttttcactgc tgtctctgca gtctgtgtga
    11161 caggtctctc aacccttcca gtagctcaca cctataatat atggggccaa ataatctgtt
    11221 tgctcttgat tcagatcggt ggtctaggtc tcatgacctt tattggggtt ttctatatcc
    11281 agagcaagca aaagcttagt cttcgtagcc gtgcaactat tcaggatagt tttagttatg
    11341 gagaaactcg atctttgaga aagtttgtct attctatttt tctcacgacc tttttggttg
    11401 agagcttggg agctattttg cttagttttc gccttattcc tcaacttggc tggggacgtg
    11461 gtctttttag ttccattttt ctagcgatct cagccttctg taatgccggt tttgataatt
    11521 tagggagcac cagtttattt gcttttcaga ccgatttact ggtcaatctg gtgattgcag
    11581 gcttgattat tacaggcggc cttggtttta tggtctggtt tgatttggct ggtcatgtag
    11641 gaagaaagaa aaaaggacgt ctgcactttc atacgaagct tgtattatta ttgactatag
    11701 gtttgttgtt atttggaacg gcaactactc tctttcttga gtggaacaat gctggaacga
    11761 ttggcaatct ccctgttgcc gataaggttt tagttagctt ttttcaaaca gtgacgatgc
    11821 gaacagctgg cttttctacg atagattata ctcaggctca tcctgtgact cttttgattt
    11881 atatcttaca gatgtttcta ggtggggcac ctggaggaac agctggggga ctcaagatta
    11941 cgacattttt tgtcctcttg gtctttgcac gaagtgagct tctaggcttg cctcatgcca
    12001 atgttgcgag acgaacgatt gcgccgcgaa cggttcaaaa atcctttagt gtctttatta
    12061 tctttttgat gagcttcttg ataggattga ttctgctagg gataacagcc aaaggcaatc
    12121 ctccctttat ccacctcata tttgaaacta tttcagctct tagtacagtt ggtgtaacgg
    12181 caaatctgac tcctgacctt gggaaattgg ctctcagtgt tatcatgcca cttatgttta
    12241 tgggacgaat tggtcccttg accttgtttg ttagcttggc agattaccat ccagaaaaga
    12301 aagatatgat tcactatatg aaagcagata ttagtattgg ttaagaaagg aaagagcatg
    12361 tcagatcgta cgattggaat tttgggcttg ggaatttttg ggagcagtgt cctagctgcc
    12421 ctagccaagc aggatatgaa tattatcgct attgatgacc acgcagagcg catcaatcag
    12481 tttgagccag ttttggcgcg tggagtgatt ggtgacatca cagatgaaga attattgaga
    12541 tcagcaggga ttgatacctg cgataccgtt gtagtcgcga caggtgaaaa tctggagtcg
    12601 agtgtgcttg cggttatgca ctgtaagagt ttgggggtac cgactgttat tgctaaggtc
    12661 aaaagtcaga ccgctaagaa agtgctagaa aagattggag ctgactcggt tatctcgcca
    12721 gagtatgaaa tggggcagtc tctagcacag accattcttt tccataatag tgttgatgtc
    12781 tttcagttgg ataaaaatgt gtctatcgtg gagatgaaaa ttcctcagtt ttgggcaggt
    12841 caaagtctga gtaaattaga cctccgtggc aaatacaatc tgaatatttt gggtttccga
    12901 gagcaggaaa attccccatt ggatgttgaa tttggaccag atgacctctt gaaagcagat
    12961 acctatattt tggcagtcat caacaaccag tatttggata ccctagtagc attgaattcg
    13021 taaagaggga tgacccctct tttttgatgc ccaagatggc aaatagagac agaagcccct
    13081 tgtcttctag taaaagttct tcaaaggctg gactttatgg taaaatagaa ggaagtgaca
    13141 agagagagta atactcaatg aaaatcaaag atcaaactag gaaactagct acgggctgct
    13201 caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagctcaaaa cactgttttg
    13261 aggttgcaga tagaactgac gaagtcagta acatctatac ggcaaggcga cgttgacgcg
    13321 gtttgaagag attttcgaag agtataagaa aaaatcagtc ccctaaagga gcagattatg
    13381 aagttattgt ctatcgccat ttctagctat aatgcagcag cctatcttca ttactgtgtg
    13441 gagtcgctag tgattggctg ggaccaggtc ggaaatttct ccaaaggcca gtatatcatg
    13501 atgcactcgc tgatttatcg gacagatttg ttgcgtgcta gccagttcta attgcctgaa
    13561 catacttttt atgtcgataa tctctttgtc tttacgcccc ttcagcaggt caagaccatg
    13621 tactatctgc ctgtcgattt ctatcgttat ttgattgggc gtgagggcca gtctgtcaat
    13681 gagcaagtga tgattaagtg cattgaccag caactcaagg tcaatcgact cttggtagac
    13741 cagcttgatt tgtcccaagt gagtcatccc aaaatgcgag aatatctgct gaatcatatt
    13801 gaaatcacga cggtgatttc cagtaccctg ctcaaccgat ctgaaacagc ggagcatctg
    13861 gcaaaaaaac gccaattgtg gacctatatt cagcagaaaa atccagaagt ctttcaggct
    13921 attcgtaaga ccatgttgag ccgtttgacc aaacattctg tcttgccaga tcgcaaactg
    13981 tccaatgtcg tctatcaaat caccaaatct gtttatggat ttaattaata taagtgtttt
    14041 ataagaggga tttaagaaaa attttaactt tttcttagtc ctttttaatt tcaggagatt
    14101 atactagagt catcaaataa agaaagactc taaggagaat cctatgaaat tcaatccaaa
    14161 tcaaagatat actcgttggt ctattcgccg tctcagtgtc ggtgttgcct cagttgttgt
    14221 ggctagtggc ttctttgtcc tagttggtca gccaagttct gtacgtgccg atgtggtcaa
    14281 tccgacccca ggtcaagtct tacctgaaga gacatcggga acgaaagagg gtgacttatc
    14341 agaaaaacca ggagacaccg ttctcactca agcgaaacct gagggcgtta ctggaaatac
    14401 gaattcactt ccgacaccta cagaaagaac tgaagtgagc gaggaaacaa actcttctag
    14461 tctggataca ctttttgaaa aagatgaaga agctcaaaaa aatccagagc taacagatgt
    14521 cttaaaagaa actgtagata cagctgatgt ggatgggaca caagcaagtc cagcagaaac
    14581 tactcctgaa caagtaaaag gtggagtgaa agaaaataca aaagacagca tcgatgttcc
    14641 tgctgcttat cttgaaaaag ctgaagggaa aggtcctttc actgccggtg taaaccaagt
    14701 aattccttat gaactattcg ctggtgatgg tatgttaact cgtctattac taaaagcttc
    14761 ggataatgct ccttggtctg acaatggtac tgctaaaaat cctgctttac ctcctcttga
    14821 aggattaaca aaagggaaat acttctatga agtagactta aatggcaata ctgttggtaa
    14881 acaaggtcaa gctttaattg atcaacttcg cgctaatggt actcaaactt ataaagctac
    14941 tgttaaagtt tacggaaata aagacggtaa agctgacttg actaatctag ttgctactaa
    15001 aaatgtagac atcaacatca atggattagt tgctaaagaa actgtcgaaa aagctgttaa
    15061 agataatgtt aaagacagta tcgatgttcc agcagcctac ctagaaaaag ccaagggtga
    15121 aggtccattc acagcaggtg tcaaccatgt gattccatac gaactcttcg caggtgatgg
    15181 tatgttgact cgtctcttgc tcaaggcatc tgataaggca ccatggtcag ataacggtga
    15241 cgctaaaaac ccagccctat ctccactagg tgaaaacgtg aagaccaaag gtcaatactt
    15301 ctatcaagta gccttggacg gaaatgtagc tggcaaagaa aaacaagcgc tcattgacca
    15361 gttccgagca aatggtactc aaacttacag cgctacagtc aatgtctatg gtaacaaaga
    15421 cggtaaaccg gacttggaca acatcgtagc aactaaaaaa gtcactatta acataaacgg
    15481 tttaatttct aaagaaacag ttcaaaaagc cgttgcagac aacgttaagg acagtatcga
    15541 tgttccagca gcctatttag aaaa
//
LOCUS       DAVHIT010000047        15167 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVHIT010000047 DAVHIT010000000
VERSION     DAVHIT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.048"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            9..731
                     /locus_tag="VVN16_001289"
                     /pseudo
     CDS             9..731
                     /locus_tag="VVN16_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            881..1135
                     /gene="blpM"
                     /locus_tag="VVN16_001290"
     CDS             881..1135
                     /gene="blpM"
                     /locus_tag="VVN16_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU5572675.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            1151..1354
                     /gene="blpN"
                     /locus_tag="VVN16_001291"
     CDS             1151..1354
                     /gene="blpN"
                     /locus_tag="VVN16_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU5572676.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            1598..1747
                     /gene="blpO"
                     /locus_tag="VVN16_001292"
     CDS             1598..1747
                     /gene="blpO"
                     /locus_tag="VVN16_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU5572677.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1851..1970
                     /locus_tag="VVN16_001293"
     CDS             1851..1970
                     /locus_tag="VVN16_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU5572678.1"
                     /translation="MDFKSFIIGLVVGIFGPCMDDLIRKIFSKSSKKDTDSTL"
     gene            2438..2809
                     /locus_tag="VVN16_001294"
     CDS             2438..2809
                     /locus_tag="VVN16_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572679.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQIMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLIIILFLIWGLKYYKK"
     gene            2970..3939
                     /locus_tag="VVN16_001295"
                     /pseudo
     CDS             2970..3939
                     /locus_tag="VVN16_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4246..4479
                     /locus_tag="VVN16_001296"
     CDS             4246..4479
                     /locus_tag="VVN16_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HEU5572680.1"
                     /translation="MDTKMMSQFAVMDNEMLARIEGGDVSDIYRGYANQVSPFGSYPP
                     ILKNSGPFPVSGYCPRGYHDRGYIGAGFHLCGI"
     gene            4511..5200
                     /locus_tag="VVN16_001297"
     CDS             4511..5200
                     /locus_tag="VVN16_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5572681.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            5242..5475
                     /locus_tag="VVN16_001298"
     CDS             5242..5475
                     /locus_tag="VVN16_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572682.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            5626..6237
                     /locus_tag="VVN16_001299"
     CDS             5626..6237
                     /locus_tag="VVN16_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5572683.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            6398..7192
                     /locus_tag="VVN16_001300"
     CDS             6398..7192
                     /locus_tag="VVN16_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU5572684.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            7189..7824
                     /gene="trmB"
                     /locus_tag="VVN16_001301"
     CDS             7189..7824
                     /gene="trmB"
                     /locus_tag="VVN16_001301"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU5572685.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            7950..8429
                     /gene="rimP"
                     /locus_tag="VVN16_001302"
     CDS             7950..8429
                     /gene="rimP"
                     /locus_tag="VVN16_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU5572686.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            8473..9609
                     /gene="nusA"
                     /locus_tag="VVN16_001303"
     CDS             8473..9609
                     /gene="nusA"
                     /locus_tag="VVN16_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU5572687.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            9631..9924
                     /locus_tag="VVN16_001304"
     CDS             9631..9924
                     /locus_tag="VVN16_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU5572688.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            9917..10216
                     /locus_tag="VVN16_001305"
     CDS             9917..10216
                     /locus_tag="VVN16_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU5572689.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            10233..13025
                     /gene="infB"
                     /locus_tag="VVN16_001306"
     CDS             10233..13025
                     /gene="infB"
                     /locus_tag="VVN16_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5572690.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKSAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRTLMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            13276..13626
                     /gene="rbfA"
                     /locus_tag="VVN16_001307"
     CDS             13276..13626
                     /gene="rbfA"
                     /locus_tag="VVN16_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5572691.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            13905..14684
                     /locus_tag="VVN16_001308"
     CDS             13905..14684
                     /locus_tag="VVN16_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572692.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEALWTSREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            14686..14868
                     /locus_tag="VVN16_001309"
     CDS             14686..14868
                     /locus_tag="VVN16_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572693.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            14865..15017
                     /locus_tag="VVN16_001310"
     CDS             14865..15017
                     /locus_tag="VVN16_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572694.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKIVFKILD
                     WLFKLI"
BASE COUNT         4766 a         2584 c         3377 g         4440 t
ORIGIN      
        1 ttgaagagat gttagctgta ttaaaaacag cttatcaact taaacacgca aaaggtggac
       61 gaaaacctaa attaagccta gaagaccttc ttatggccac tcttcaatat gtgtgagaac
      121 cgcacttatg aagaaattgc ggctgatctt ggtattcacg aaagcaactt aatccgtcgg
      181 agccaatggg ttgaagtaac tcttgttcaa agtggtgtta cgatttcaaa aactcatctt
      241 agtgctgagg atacggtgat tgtggatgca acagaggtaa aaataaatcg tcctaaaaaa
      301 atcaactagc caattattct ggtaaaaaga aatttcacgc tatgaaggct caagcgattg
      361 tcacaagtca agggagaatt gtttctttgg atatcactgt gaactattgc cacgatatga
      421 agttgttcaa aatgagtcgc agaaatatcg gacaaactgg taaaaccttg gctgacagta
      481 attatcaagg gctcatgaag atatatcctc aagcacaaac tccacgtaaa tccagcaaac
      541 tcaaaccgct aacagttaaa gataaagcct ataaccatgc gttatctaag gagagaagca
      601 aggttgagag catccttgcc aaagtaaaaa cgtttaaaat gttttcaaca acctatcgaa
      661 atcatcgtaa acgcttcgga ttacgaatga atttgattgc tggcattatc aatcatgaac
      721 taggattcta gttttgcagg aagtctaatg taatttttgc aattcaagac gtttcgatga
      781 ctattcaaga tctggatgaa aaattttaaa aaacgatgat atactaaact tgtcaaagtt
      841 acaacaagac aaaaattaaa aataaaaaag agtatttgtc atggatacaa aaattatgga
      901 acaatttcat gaaatggata taactatgtt atctagtatt gaaggaggca agaataattg
      961 gcaaactaat gtcttagaag gtggtggtgc tgcttttggt ggctgggggc tagggacagc
     1021 tatttgtgct gcgagtggtg ttggagcacc atttatggga gcatgtggat acataggagc
     1081 taaatttggt gtggatcttt gggcaggagt aacaggggca acgggtggat tttaataaaa
     1141 ggagacttgt atgaatactt attgtaatat aaatgaaaca atgctgagtg aggtttatgg
     1201 aggtaattcc ggaggagcag ctgtagttgc tgctttaggt tgtgcagcgg gtggagtgaa
     1261 atatgggaga cttctaggac catggggcgc tgcaatagga ggaattggag gagcagtggt
     1321 ttgtggatat ttagcctata ccgctacatc ataaaaatga aagctaaatt ttatgaaggc
     1381 tatattcttt attattctct tcgcttttca aacctatctt atttatctat ccataagtat
     1441 ctccgataaa aaacaaaaga ctattgaatt aacatcattg aattgttttg tcatattatt
     1501 tctaatctat gataaattaa tttttctctt tattgcctat gtttttttga taatttttat
     1561 attaaacttg ttccgtaact gaaaagtgat atactatatg gatacaaaaa tgatgtcaca
     1621 atttgcagtt atggataatg aaatgcttgc ttgcgttgaa ggtggagata ttgattgggg
     1681 aagaaaaatt agttgtgcag caggggttgc atatggcgca attgatgggt gtgcaacaac
     1741 ggtttgacat ttctattggg accatttgct ataggaatag gtgtaactgg tgctgcaggt
     1801 ggagctatac ttggaggtgt agcctatgca gcaacatgtt ggtggtaatt atggatttta
     1861 aaagttttat tattggttta gtagttggta tatttggtcc ttgtatggat gacttaatta
     1921 gaaaaatatt ttcaaaatct tccaaaaagg atacggatag tactctctaa aaaataaatt
     1981 tctcccctgt atttgctagc ttaatttaca ggggagtttc tttaatattg tttcgcctgt
     2041 caaaatgata tgtgatagca ggatgaaaag tctacaaaca agttggtatc aatacaatat
     2101 aaagaggaaa gtatgttttt catgcttgct tttttaattt ttacaattca agaggttttg
     2161 atgaccattt atgatttgag tgatccaagg tcaaaataag tgctatacta gcagtgtaaa
     2221 tgttcttgct caactaagat aaatcgcaca tttaggagga aaacttatga agaaaaaaat
     2281 attgattatt ttcgttctgt atctgatcat gtccatcttt ctttatccgc ttagggagag
     2341 tatatggtat aatctatttt ataccatagc ctatatgatt gcggttatga tctatttttc
     2401 tttaattaaa aagaaagaaa agaaatgaaa gattttcttg attaaaaaga atcatattct
     2461 ttgtgagatt gttcctgggt cagttgcctt tgcttgtttc tacttatcta tttctatttc
     2521 gtcagttttc caatttcttt tagtggttat taacttggct tctattttgg tcactgttta
     2581 cctcactagg gaaatgcgga taaaagagtt tgaagacgat gatttggtta gtcctagaac
     2641 taatcaaatc atgttcatcg gcttgacagg ttttatgtct attatttgtt tgtatagagg
     2701 gatcacagca ggagaatcct accaacaaat aattgcctac attggtgcta ttctctgctt
     2761 gattattata ctttttctca tttggggctt gaagtattat aaaaagtaga gcttaataaa
     2821 gtataaaggg gtaagtgttt ttgaaatact taccctttta taatgaagtt tttgcaattc
     2881 aagatgtttc gatgaccatt taagatttag atgaatttgt aatcttaaat aaggtaagat
     2941 aaagataaca aatgtaaagg agtattctta tgaagaaaca aaagataaaa tcgctattac
     3001 ttgtaccact tgtatttgca tctattactg caggtcttgc atatgcagaa gaacagccta
     3061 ttacttcatc tacccaggcg aatgatagtt ctcttgtggc gccagatttg ccatcggcta
     3121 aggaaaaggt ggagcataag ccttctgttg taaccccaga agagtatgag aagaatgtta
     3181 cggactttaa aaagatagat attgaagctg ttcgtcaatc ttttacaggg gatcaattgg
     3241 aacatattat ttattttggt agaaaaactt gctctcattg tcgtcaattt tctcctgaat
     3301 tgaaagaatt taataacttg attgaaaaga agttagagta ttatgatttg gatggtaagg
     3361 attttgatgg ggaagcgaga gagtttctat ttttaaaaag tcggtattcc aggaacacca
     3421 acaattttgt atttaagaaa tggacatccg acatctggat gggttggtga cggagcaact
     3481 gcacaacagg tgtatgatta tatgtattcg agaaactcac ctaagcaaac ggagacagtg
     3541 aaatcttctg aggaaacagt aactgaaagt gtccgtgatg ataaaacagg agaggtgaag
     3601 aattcaaagg aaaattcgac taataacagt gataacagag agccattgaa ggataatatt
     3661 cctccttcta acgctataac tgttcagtct gaagatttgg taatgataga taagaagaag
     3721 cgaggggagt catctcagac gatttctgaa aatactgaga agttacaaac tcctttatcc
     3781 aaggttgagg ggaatgattc tccctccaaa accagtcatt tgagtaaaca attactacct
     3841 aatacaggag tggaagaaag ttacaatcta ttacgtctag cggttgcact cttaattact
     3901 tcaggtatga ttaagtggag acaaagaatt cataaataaa aatagatggt aaacaggtga
     3961 aatggtacta tttgtttgta ataacatatg gctagaattt gcctatagac aagagtgaga
     4021 aaataggtgt atttatttta tatataaaag attataattg tatatgaaat gaatggtaaa
     4081 aggtaaagta tttctgaaat gctttctttt tcgactattt taacgcctca agatgttttg
     4141 atgacaattc aagatttgat gaaaaaaatt aataaacgat gatatactaa acttgtcaag
     4201 gttgcaacag gacaaaaatt aaacataaaa aaggagatat tcgtcatgga tacaaaaatg
     4261 atgtcacaat ttgcagttat ggataatgaa atgcttgctc gtatagaggg tggggatgta
     4321 tctgatattt atagagggta cgctaatcag gtaagtccat tcggttctta ccctcctata
     4381 ttaaaaaatt caggtccttt tccagtaagt ggttactgcc cgcgcggtta tcatgaccgt
     4441 ggttatatag gagcaggttt tcatttatgt ggaatatagg ctgaaataca agaaatgagg
     4501 aagaatttct atgaaaaagt atcaacttct attcaaaata agtgcagttt tctcttactt
     4561 atttttcgta tttggtcttt ctcagatgac gcttattatt caaaattatt ggcaattttc
     4621 ttcccagatt ggcaatttcg tctggattca aaatttcttg agtttgctat ttagcggagt
     4681 catgatttgg attctggtta agacaggtca tggttatctc tttcacattc caagaaaaaa
     4741 atggctttgg tattcgattt tgacagtatt agtggtagtg ctccagatct cttttaacgt
     4801 tcagacagct aaacatgttc agtcaactgc tgaaggttgg gctgtattga taggttatag
     4861 tgggaccaac tttgctgagc taggtatcta tgtaaccctg tttttcctga ctccactaat
     4921 ggaagagttg atttataggg gattacttca acacgccttc tttaagcatt cgcgatttgg
     4981 ccttgatttg cttcttcctt ccattttgtt tgctcttcct cattttttga gtctgcctag
     5041 tctgttagat atcttcgtct ttgcaacatt tggaatcatc tttgccggtt tgacccgcta
     5101 taccaagagt atttatccat cctatgcggt gcatgtgatc aataatattg tagcgacctt
     5161 cccgtttttg ctcacttttc tacatagggt cttggggtaa aaaaacaaaa ggcttgcttt
     5221 tcagccatag aggaggtcat catgtataaa cacttatttt tcctagattc caaaacttta
     5281 gatcggttga caccctatat tctagtcttg gcttctgaca ccattgcttt taatgttttt
     5341 gtgctaacct ttgtatctgc ggtggttttt aatttcctaa attccatgct agctttaatg
     5401 gctatattca taggggctgg ctatgtggtc ggattttggt tactaatact caatgaaaat
     5461 caaagagcaa actaggaaac tagccgcagg ctgtacttga gtacggcaag gcgacgttga
     5521 cgtggtttga atttgatttt cgaagagtat aaaatggttt gttttggaaa ggctagaact
     5581 aaagaatgac gtgtaggaag tctgtttgtt gaggaggata attttatgga gttttttgat
     5641 aaatttcatg ccttgtgttt tggattttta gtactaataa ttgtcattac agttccttat
     5701 acgattaacc atggggattt ttttcaaaat gaatctgcat tgattattgt aagtcttctt
     5761 gtaacctcgc tgagtgttgc ttatgctaga aagtttgaaa tgatttcttt tgggatgtta
     5821 agcaagaaac aacttttgct tttcattgca atctttcttc taagtgtact tgagacgctg
     5881 gtttatattc atttcttcgc tgtttcttct ggctcagggg tccaacactt ggcggaagtc
     5941 agcagaggaa tttccctgtc tttgattttg actacctcag tttttggccc catccaggag
     6001 gaactcattt tcagaggact tcttcaaggt gcggtttttg acaattcttg gttaggactt
     6061 gtgctaactt cctctctctt ttctttcatg catggacctt ctaatgtccc ttcgtttatt
     6121 ttttatctac ttgggggctt gttgctgggc cttgcttata aaaagagcca aaacttatgg
     6181 gtttctactc tagtccacat gttttacaac agttggccac tcctatatta tttataaaaa
     6241 ttatgaaaag gtaagcagaa gacggtgctt acctttttca agctataatg atacccaagc
     6301 caatccagag gcttttcttt gagaatcggg ttagagtggt tgacgaatag gccaaaaact
     6361 agtagaatag taaggaaact ttatacggag gaaagaaatg gatttgggtg ataatgagct
     6421 aacactgact cccatacctg ggaaaagtgg caaggcttat atgggtagct atcctgatgg
     6481 gaagcgcatc tttgtaaaaa tgaacacctc tccaatccta cctggtctag ctagagaaca
     6541 aattgctcca caattattat ggagtcgtcg tttggcagat gggcgtgata tgtgtgctca
     6601 agaatggttg acaggcaaga tattgacccc ctatgatatg aatcgtaagc aaatcgtcaa
     6661 tattttaacc cgtcttcatc gctcacgtcc gttgatgaca caattgagtc gtttgggcta
     6721 tgccatggaa acacctgtag atttactaca gtcttggcaa gaaacggctc cagatgcttt
     6781 gcgtaaaaat cattttatca gtgaagtgat ggctgattta cgtcagacta ttccaggatt
     6841 tagagaggac catgcgacca ttgtccatgg agatgtacga catagtaatt ggattgagac
     6901 agatagtggc ttgatttatt tagtagattg ggattcggtt cgcttgaccg atcgcatgtt
     6961 tgatgtggcc catatgctct gccattatat ttcagaacat cagtggaagg aatggttgac
     7021 ctactacggt tacaagtaca atcaaacggt attaagtaaa ttgtattggt atggtcaatt
     7081 gtcttatttg agtcagattt ccaagtatta tatgaaccaa gatttagaaa atgtcaatcg
     7141 ggagattcat ggtttgcgtc atttccgaga caagtatgga aagagaagat gagagttaga
     7201 aatcgtaaag gggcaacaga attactagag gcaaatcccc agtatgtggt cctcaatccc
     7261 ttggaagcca aggcaaaatg gcgggacttg tttggcaatg ataatcccat tcatgtggaa
     7321 gttggaagtg gaaagggtgc ctttgtttca ggtatggcca agcaaaaccc tgacatcaac
     7381 tatatcggga ttgatattca aaagtctgtt ttgagctacg ctttggacaa ggtgcttgaa
     7441 gctggagtgt ctaacatcaa gctcttgtgg gtagatggtt ctgacttgac tgactacttt
     7501 gaagacggtg agattgatcg cttgtatctg aacttttcag atccatggcc gaaaaaacgc
     7561 catgaaaagc gtcgtttgac ctacaagacc ttcttggata ccttcaaacg tatcttgcct
     7621 gaaaatggag aaattcattt caagacggat aaccgtggct tgtttgagta cagtttagtg
     7681 agcttttctc aatatggcat gaaactcaat ggtgtctggt tagatttgca tgccagtgat
     7741 tttgaaggca atgtcatgac agaatacgag caaaaattct caaacaaggg gcaagttatc
     7801 taccgagttg aggcagaatt ttaagagata acctaaaatt aggctgtaca agtgcttttg
     7861 ctttacataa gttggcaaac gtgctatact gatagtaaga atatgaaaag tgaggcgggg
     7921 aaatatcttc gcctcttgct tatgaggagg tggacgcaat cgcaacaatc gtagaattag
     7981 tcagagaagt tgtagaacct gtcatagaag ctccttttga actcgtggat atcgagtatg
     8041 gaaagattgg cagtgacatg attctcagta tttttgtaga taaacccgaa ggaattacct
     8101 tgaacgacac ggcagacttg acagaaatga tcagtcctgt cctagacacc atcaagccag
     8161 atcccttccc agaacaatat ttcctagaaa ttaccagtcc aggtttggaa cgtcctttga
     8221 aaaccaagga tgccgtcgct ggagcggttg gaaaatacat ccatgtcggg ctctaccaag
     8281 ccatcgataa gcaaaaggtc tttgaaggaa ccttgttggc cttcgaagag gacgagttga
     8341 ctatggaata tatggacaag acgcgtaaga aaaccgtcca aattccatac agtttagtat
     8401 caaaagcacg tttagcagtt aaattataga aaaagaaagg atagcttttg aggattcaaa
     8461 agtgaagaaa acatgagtaa agaaatgcta gaggccttcc gcattttgga agaagacaag
     8521 ggaatcaaaa aagaagatat catcgacgca gtagtagagt cgcttcgttc cgcttatcgc
     8581 agacgctatg gtcagtcaga cagcgtagct attgacttca acgaaaaaac aggtgacttt
     8641 acagtttata ctgtccgtga agttgttgat gaagtatttg atagccgttt ggaaatcagt
     8701 ttgaaagatg ctcttgccat taattcagct tatgaacttg gagacaaaat caagtttgaa
     8761 gaagcaccag ctgagtttgg tcgtgtagca gcccaatctg ccaaacaaac catcatggaa
     8821 aaaatgcgca agcaaacacg tgccatcact tacaatactt acaaagaaca tgagcaagaa
     8881 atcatgtctg gtacagtaga acgctttgac aatcgcttta tctatgtcaa ccttggtagc
     8941 atcgaagccc aattgtcaaa acaagaccaa attcctggag aagtttttgc ttctcatgat
     9001 cgtatcgaag tttatgttta caaggttgaa gacaaccctc gtggtgtgaa cgtctttgtt
     9061 agccgtagcc atccagaaat gatcaaacgt ttgatggagc aagaaattcc agaagtttat
     9121 gatggaactg ttgaaatcat gagcgtggct cgtgaagcag gtgaccgtac gaaggttgct
     9181 gttcgtagcc acaatccaaa cgtggatgct atcggtacaa tcgttggacg tggtggtgct
     9241 aatatcaaga agattactag caaattccac ccagctcgtt acgatgctaa aaatgaccgc
     9301 atggtaccaa tcgaagaaaa tatcgatgtt atcgagtggg tagcagatcc agctgaattt
     9361 atctacaatg ccatcgctcc tgctgaggtt gaccaagtta tctttgatga aaacgacagc
     9421 aaacgtgcct tggtggttgt tccagataac aagctttctc ttgccattgg tcgtcgtgga
     9481 caaaacgtgc gcttggcggc tcacttgact ggttaccgta tcgatatcaa gtctgctagc
     9541 gaatttgaag ccatggaaga cgctgcttca gtagagttgg aagtagaaaa cgatactgta
     9601 gaagaataaa agctgctaga ggagggaaag atgaaaacaa gaaaaatccc tttgcgcaag
     9661 tctgttgtgt ctaacgaagt gattgataag cgtgatttgc tccgcattgt caagaacaag
     9721 gaaggacaag tctttattga tcctacgggc aaggccaatg gccgcggcgc ttatatcaaa
     9781 ctagacaatg cagaagccct agaggcgaaa aagaagaagg tctttaaccg cagctttagc
     9841 atggaagtgg aagaaagctt ttatgacgag ttgatcgctt atgtggatca caaagtgaaa
     9901 agaagagagt tgggacttga ataagcaaaa gataagtaat ctcttggggc ttgctcagcg
     9961 agcagggcgc atcatatcgg gtgaagaatt ggtggtcaag gccattcaag acggcaaggc
    10021 caagttggtc tttctagctc atgatgctgg acccaatctg accaagaaga ttcaagataa
    10081 aagtcattat tatcaagtag aaattgtaac cgtgttttca acactggaat taagcatagc
    10141 agtcgggaaa tcgagaaagg ttttggctgt aacagatgct ggatttacaa agaaaatgag
    10201 gtctcttatg gaatagaaga ggaggacatg atttgtctaa gaaaagattg tacgaaatcg
    10261 caaaagaact tggaaaagaa agtaaagaag ttgtagcgcg tgcaaaagag ttgggcttgg
    10321 atgtgaaaag ccactcatca agtgtggaag aagctgtcgc tgcaaaaatt gctgccagct
    10381 ttaagtctgc agctgctccg aaagtagaag caaaacctgc agcaccaaaa gtaagtgcag
    10441 aaaagaaagc cgaaaaatct gagccagcta aaccagctgt agctaaggaa gaggcaaaac
    10501 cggctgagcc agttgctccg aaaacagaaa aagtagcagc gaaaccgcaa agccgtaatt
    10561 tcaaggctga gcgtgaagcc cgtgctaaag agcaggcaga acgacgcaag caaaataagg
    10621 gcaataaccg tgaccaacaa caaaacggaa accgtcagaa aaacgacggc cgtaatggtg
    10681 gaaaacaagg tcaaagcaac cgcgacaatc gtcgctttaa tgaccaagct aagaagcagc
    10741 aaggtcagca aaaacgtaga aatgagcgcc gtcagcaaga ggataaacgt tcaaatcaag
    10801 cggctccacg tattgacttt aaagcccgtg cagcagccct aaaagcagag caaaatgcag
    10861 agtacgctcg ttcaagtgag gaacgcttca agcagtatca ggctgctaaa gaagccttgg
    10921 ctcaagctaa caaacgcaag gaaccagagg aaatctttga agaagcggct aagttagctg
    10981 aacaagcaca gcaagttcaa gcagtggttg aagtcgtccc tgagaaaaaa gaacctgcag
    11041 tggatacacg tcgtaaaaaa caagctcgac cagacaaaaa tcgtgacgat tatgatcatg
    11101 aagaagatgg tcctagaaaa caacaaaaga atcgaagtag tcaaaatcaa gtgagaaatc
    11161 aaaagaatag taactggaat aacaacaaaa agaacaaaaa aggcaataac aagaacaacc
    11221 gtaatcagac tccaaaacct gttacggagc gtaaattcca tgaattgcca acagaatttg
    11281 aatatacaga tggtatgacc gttgcggaaa tcgcaaaacg tatcaaacgt gaaccagctg
    11341 aaatcgttaa gaaacttttc atgatgggtg tcatggccac acaaaaccaa tccttagatg
    11401 gggaaacaat tgaactcctc atggtggatt acggtatcga agccaaacaa aaggttgaag
    11461 tggataatgc tgacatcgaa cgtttctttg tcgaagatgg ttatctcaat gaagatgaat
    11521 tggttgagcg tccaccagtt gttactatca tgggacacgt tgaccacggt aaaacaaccc
    11581 ttttggatac tcttcgtaac tcacgtgttg cgacaggtga agcaggtggt attactcagc
    11641 atatcggtgc ctaccaaatc gtggaaaatg gtaagaagat taccttcctt gatacaccag
    11701 gacacgcggc ctttacatca atgcgtgcgc gtggtgcttc tgttaccgat attacgatct
    11761 tggtcgtagc ggcagatgac ggggttatgc ctcagactat cgaagccatt aaccactcaa
    11821 aagcggccaa cgttccaatc atcgtagcca ttaacaagat tgataaacca ggtgctaacc
    11881 cagaacgcgt tatcggtgaa ttggcagagc atggagttat gtcaaccgct tggggtggag
    11941 attctgaatt tgttgaaatc tcagctaaat tcaaccaaaa tatcgaagaa ttgttggaaa
    12001 cagtccttct tgtagctgaa atccaagaac tcaaggcaga cccaacagtg cgtgcaatcg
    12061 gtacggttat cgaagcgcgc ttggataaag gaaaaggtgc ggtcgcaacc cttcttgtac
    12121 aacaaggtac cttgaatgtt caagacccaa tcgttgtcgg aaataccttc ggtcgtgtcc
    12181 gtgctatgac caacgacctt ggtcgtcgtg ttaaagttgc tggaccatca acaccagtct
    12241 ctatcacagg tttgaacgaa gcaccgatgg cgggtgacca ctttgccgtt tacgaggatg
    12301 aaaaatctgc gcgtgcagca ggtgaagagc gtgccaaacg taccctcatg aaacaacgtc
    12361 aagctaccca acgtgttagc cttgaaaacc tctttgatac ccttaaagct ggggaactca
    12421 aatctgttaa tgttatcatc aaggctgatg tacaaggttc tgttgaagcc ctttctgcct
    12481 cacttcaaaa gattgacgtg gaaggtgtca aagtgactat cgtccactca gcggtcggtg
    12541 ctatcaacga atcagacgtg acccttgccg aagcttcaaa tgcctttatc gttggtttca
    12601 acgtacgccc tacaccacaa gctcgtcaac aagcagaagc tgacgatgtg gaaatccgtc
    12661 ttcacagcat tatctacaag gttatcgaag agatggaaga agctatgaaa gggatgcttg
    12721 atccagaatt tgaagaaaaa gttattggtg aagcggttat ccgtgaaacc ttcaaggtgt
    12781 ctaaagtggg aactatcggt ggatttatgg ttatcaacgg taaggttgcc cgtgactcta
    12841 aagtccgtgt tatccgtgat ggtgtcgtta tctatgatgg tgaactcgca agcttgaaac
    12901 actataaaga cgacgtgaaa gaagtgacaa acggtcgtga aggtggattg atgatcgacg
    12961 gctacaatga tattaagatg gatgatgtga ttgaggcgta tgtcatggaa gaaatcaaga
    13021 gataagattt tttgctcctt tcttaggtgg tgagggacgc aagcaaaccg atggtttcat
    13081 tgcttatttt tgagcctagg gtctcaaaaa tcccctgtga tgggactgat aaatcagttc
    13141 catcactttc accacggcga aagaagcaga tgacttcaaa ttgaacttcg tttcaattta
    13201 aactgaaaat caagaagttt aaaatagcta ggtctgctgg cctagctttt ggttcaaagt
    13261 agagaaagga atatcatggc aaatcatttc cgtacagatc gtgtgggcat ggaaatcaag
    13321 cgtgaagtca atgagatttt gcaaaagaaa gtccgtgatc cacgtgtcca aggtgtgacc
    13381 atcacagatg ttcagatgct gggtgacttg tctgttgcca aggtttatta caccattttg
    13441 agtaaccttg cttcggataa ccaaaaagcc caaatcgggc ttgaaaaagc aactggtacc
    13501 atcaaacgtg aacttggtcg caatttgaaa ttgtacaaaa tcccagattt gaccttcgtc
    13561 aaagacgagt ccatcgagta tggaaacaag attgacgaga tgctacgcaa tctggataag
    13621 aactaaagaa gaggggttgc ccctcttttt tggtggagga aaataggttg aatttgaaat
    13681 ggaaaaatat tcttttataa tagattgaaa ctagaatagt acacctctac ttctaaaaca
    13741 ttgttagaaa tcgatttgac tgtcctgatc gatttgtcct gttcttgttt cattttaata
    13801 taaaaaaggg gttctgtatt ttttaatgtt atctaattag aaaatgcttt ttttgtagga
    13861 aatataatat gataaggtgc aaaaaagaaa taaggagttt gtatatggct gaacaagact
    13921 tagctatgca agtattgcaa caagtggtga aactacctgt tgttaaggtt gatcgttcga
    13981 aatttttagt ggataagttt tccaaagaat tggatccaaa agatattcct accttattgg
    14041 aacaaggtcc aacgactctt ctatctcaag aaatattaga tcgtgtagct aatgcttgta
    14101 ttcgggacaa tgtattatta gcgagtggga cttctgtttt ggcaggatta cctggagggc
    14161 ttgctatggc aattaccatt ccagctgatg tggctcaatt ttatgctttc tctctgaaat
    14221 tggctcaaga attaggttat atttatggtt atgaggctct ttggacttca cgtgaggagt
    14281 taagtgaaga tgctcaaaat accctcttgc tttatctagg cgtcatgttg ggggtgaatg
    14341 gaactgctgc tttgctacgt gctggtggta taacaattgc caaacaggta atgaaaacag
    14401 tacctaataa agctttaaca aagacgcttt ggtatcctat tttgaaaaaa gtcttaagaa
    14461 tatttggtgt gaatcttacc aagggagggc tggccaaagg aatggggaaa tttattccta
    14521 tcttgggtgg tatcatttca ggtggtttaa cctttgcaac tatgaaaccg atgggtgaaa
    14581 gcttgcagaa agaattgtcc aagctagtta actatagtga agttcaatat caagaagatg
    14641 ttgaaacaat ccgaaaagag gctgaaatca tcgaaggaga gtaatatggg actcataaaa
    14701 actctagcta aaatttatgg taattacttt ttgaccgtgc aaggtgtaaa agtaatgaaa
    14761 acgataaaga aagatgacca tgtcgttgtt ggtctgggaa aactctttat tgcagataag
    14821 ctaatggata cggctcggtg gctcattaag ccagaggata aaaaatgaaa tttttctggg
    14881 gacttcttgc cattattttt atcaaaccga ttattgggat tgtgaaattc ttttggatga
    14941 tcatctcttt tgcagtccaa ttgctgtttt acaagatagt gtttaagata ttggattggc
    15001 tctttaaact tatctagatg gtaatccaag tcgcagagaa ctagcaggaa ctccactgct
    15061 agttttttat tctctttcca tatggtataa tataagcagt aaaatcattt tatactcttc
    15121 gaaaatctct tcaaaccacg tcagcttcac cttgcagtat atatgtt
//
LOCUS       DAVHIT010000048        15016 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVHIT010000048 DAVHIT010000000
VERSION     DAVHIT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.049"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(119..577)
                     /locus_tag="VVN16_001311"
     CDS             complement(119..577)
                     /locus_tag="VVN16_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU5572695.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMPAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(574..1014)
                     /locus_tag="VVN16_001312"
     CDS             complement(574..1014)
                     /locus_tag="VVN16_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572696.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRPYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1370..3319
                     /gene="mutL"
                     /locus_tag="VVN16_001313"
     CDS             1370..3319
                     /gene="mutL"
                     /locus_tag="VVN16_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU5572697.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVDQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3644..4111)
                     /locus_tag="VVN16_001314"
     CDS             complement(3644..4111)
                     /locus_tag="VVN16_001314"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU5572698.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4112..5317)
                     /locus_tag="VVN16_001315"
     CDS             complement(4112..5317)
                     /locus_tag="VVN16_001315"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU5572699.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRIDDLIQFSKKHNIPLITIKELQEYRKAYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKIIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5337..5972)
                     /gene="ribE"
                     /locus_tag="VVN16_001316"
     CDS             complement(5337..5972)
                     /gene="ribE"
                     /locus_tag="VVN16_001316"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5572700.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5957..7057)
                     /gene="ribD"
                     /locus_tag="VVN16_001317"
     CDS             complement(5957..7057)
                     /gene="ribD"
                     /locus_tag="VVN16_001317"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5572701.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGSHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMTIDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7191..7305)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7462..8055
                     /gene="ruvA"
                     /locus_tag="VVN16_001318"
     CDS             7462..8055
                     /gene="ruvA"
                     /locus_tag="VVN16_001318"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5572702.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKIAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8065..8628
                     /locus_tag="VVN16_001319"
     CDS             8065..8628
                     /locus_tag="VVN16_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5572703.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8625..9302
                     /locus_tag="VVN16_001320"
     CDS             8625..9302
                     /locus_tag="VVN16_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5572704.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9439..10470)
                     /locus_tag="VVN16_001321"
     CDS             complement(9439..10470)
                     /locus_tag="VVN16_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU5572705.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(10725..11408)
                     /locus_tag="VVN16_001322"
     CDS             complement(10725..11408)
                     /locus_tag="VVN16_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU5572706.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11420..12364)
                     /locus_tag="VVN16_001323"
     CDS             complement(11420..12364)
                     /locus_tag="VVN16_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5572707.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            complement(12525..13691)
                     /locus_tag="VVN16_001324"
     CDS             complement(12525..13691)
                     /locus_tag="VVN16_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5572708.1"
                     /translation="MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKEVK
                     QKANQEKIAFQKAGSTRQKAVTIKNYQELATLWLESYKNTVKPNTQGNVKKLIDNHIL
                     PIFGAYKLDKLTTPLIQSIINELADKTNRGEKAAFLHYDKIHALNKRILQYGVTMQAI
                     PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLL
                     ATGCRINEALALSWSDIDLDNAVVHITKTLNRDIEINSPKSKASYRDIDIDQATVSML
                     KQYKLRQTKEAWKIGQRERVVFSDFIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRH
                     THASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL"
     gene            complement(13776..>15016)
                     /locus_tag="VVN16_001325"
     CDS             complement(13776..>15016)
                     /locus_tag="VVN16_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU5572709.1"
                     /translation="SVEVVNIKGKEIVIIPVNKVDYKDKPIYLNDNIASTYFRQGTGD
                     FRCSQEQINSMLRDSAKESFDSTLIQDFSILDLDTETIKLYREKFDNINAEHPFSKLD
                     NEQFLIKINALRRDRKDDKIKPTVAGLLIFGTHNSIKEFIPHYNVEYVLKEFSENNRF
                     KDRVIYDGTWGEDNLFNFFYLVIEKLYLTLNDNSNIQENSMNRIGISKLRIAIREAFI
                     NSLIHSDYKSEKGIMIIRYPDRYIFTNGGTLRIDIKDFFSGAHSDPRNYLIQEIFRFL
                     NLCEKAGTGIPKIMEAVKESHLKYPNLRTELDSVEFTLWDTSLIDNLDIDNEYEKKIL
                     ELIIEHRFVTREALEEKLKIHKNTILKYLKKLVEKQAIDKFKSGRQYVYFITQNQDPE
                     FQKYNHINAMYSLLEEIKRK"
BASE COUNT         4247 a         2884 c         2959 g         4926 t
ORIGIN      
        1 tcgatttgac tgtcctgatc gatttatcct gttcttattt catttcacta tagtaaggtg
       61 cagcaggata aagaaaaacc gagaagtttc tttctcggat ttgcttatta tgaggttcct
      121 attttctatg ctccaagagt tgattgatgt gatctacttt tttagcctct cttttataga
      181 gaatagccca gatgatgaga tagacaatcg caaactcgat aatgagctgg aggtagaaaa
      241 tccagtggaa agggaaccaa ccagcaagag ttgctagtgg gacaaagcca gccggcataa
      301 ggaagaaatg agtcagagtg gcacgaagca tgctccagtc acggctgaat aagcggttgc
      361 caaagttgaa gagcataccg atggctgccc agataagtgt gcagtagagc aagaccaggg
      421 caccgtgaac ctgatgctga tccatcactt ggccgatgag agagtaggga tttagtggtg
      481 cgtaggtatt tggtgcataa atgagtgaaa agaggatgga gaggatgagg ccgataagaa
      541 caccggcagc tgcatcgtga aatacttgtt ttttcatagt tctaatttct ccttgatggt
      601 ttttagataa cggcgtgaag agtaggtgaa gctttcgttt tttaagaaaa tttctaccag
      661 accgtttggc gtgagcttga ggtgagagat ggaatcgata ttgatgattt ctgattggga
      721 aatttggata aaattgcttg gcaagagttt aagaacctga tagggtcgca aatcaatgct
      781 gtaggtctga ctcgcggttt ctgctagaac cttccgattc tctatataga agcgctgaat
      841 cttgccaatc tcaactagat agacctgatc atcgattttc cctttgattt tttctctttg
      901 gtccagattt tctgcgaact cgatgacttt ctggacttta tctgtcggct gaggtgcttg
      961 gacaatcagc ttttcctcct cgtaagtctc actaatctgt agttctactt tcataaattt
     1021 ctctccttgt ttttcataca aaattaatct cacttcttgt acacctttat taatactctt
     1081 caaaaatctc ttcaaaccac gtcagcgtcg tcttgccata ggatagttgt tgactttgtc
     1141 agttttatct acaacctcaa aacagtgttt tgaactgtct gcacctagct ttctagtttg
     1201 ctctttgatt tttattgagt ataccactat tttactccct ctggcaaggg actttgtcta
     1261 tgtggaggga ttgggctcct atgtggtgga gcttttctgt tctttctgaa atatggtata
     1321 atagcactaa ttaatttcta ggaaaataga tacagaaagg ggctgaaaga tgtctcatat
     1381 tattgaattg ccagagatgc tggcaaacca aatcgcggct ggagaggtca ttgaacgtcc
     1441 tgccagtgtg gtcaaagagt tggtagaaaa tgccattgac gcgggctcta gtcagattat
     1501 cattgagatt gaggaagctg gtctcaagaa ggttcaaatc acggataacg gtcatggaat
     1561 tgcccacgat gaggtggagt tggccctgcg tcgccatgcg accagtaaga taaaaaatca
     1621 agcagatctc tttcggattc ggacgcttgg ttttcgtggt gaagccttgc cttctattgc
     1681 gtctgttagt gtcttgactc tgttaacggc ggtggatggt gctagtcatg gaaccaagtt
     1741 agtcgcgcgc gggggtgaag ttgaggaagt catcccagcg actagtcctg tgggaaccaa
     1801 ggtttgtgtg gaggatctct ttttcaacac gcctgcccgt ctcaagtata tgaagagcca
     1861 gcaagcggag ttgtctcata tcattgatat tgtcaaccgt ctgggcttgg cccatcctga
     1921 gatttctttt agcttgatta gtgatggcaa ggaaatgacg cggacagcag ggactggtca
     1981 attgcgccaa gcaatcgcag ggatttacgg tttggtcagt gccaagaaga tgattgaaat
     2041 tgagaactct gacctagatt tcgaaatttc aggttttgtg tccttgcctg agttgactcg
     2101 ggctaaccgc aattatatca gcctcttcat caatggtcgt tatatcaaga acttcctgct
     2161 caatcgtgct attttggatg gtttcggaag caagcttatg gttggacgtt ttccactggc
     2221 tgtcattcac atccatatcg acccttatct agcggatgtc aatgtgcatc caactaagca
     2281 agaggtgcgg atttccaagg aaaaagaact gatgaccctg gtttcagaag ctattgcaaa
     2341 tagtctcaag gaacaaacct tgattccaga tgccttggaa aatcttgcca aatcgaccgt
     2401 gcgcaatcgt gagaaggtgg atcaaactat tctcccactc aaagaaaata cgctctacta
     2461 tgagaaaact gagccgtcaa gacctagtca aactgaagta gctgattatc aggtagaatt
     2521 gactgatgaa gggcaggatt tgaccctgtt tgccaaggaa accttggacc gattgaccaa
     2581 gccagcaaaa ctgcattttg cagagagaaa gcctgctaac tacgaccagc tagaccatcc
     2641 agagttagat cttgctagca tcgataaggc ttatgacaaa ctggagcgag aagaagcatc
     2701 cagcttccca gagttggagt ttttcggaca aatgcacggg acttatctct ttgcccaagg
     2761 gcgagatgga ctttacatca tagatcagca cgctgctcag gaacgggtca agtacgagga
     2821 gtaccgtgaa agcattggca atgttgacca gagccagcag caactcctag tgccctatat
     2881 ctttgaattt cctgcggatg atgccctgcg tctcaaggaa agaatgcctc tcttagagga
     2941 agtgggcgtc tttctagcag agtacggaga aaatcaattt attctacgtg aacatcctat
     3001 ttggatggca gaagaagaga ttgaatcagg catctatgag atgtgcgaca tgctcctttt
     3061 gaccaaggaa gtttctatca agaaataccg agcagagctg gctatcatga tgtcttgcaa
     3121 gcgatctatc aaggccaatc atcgtattga tgatcattca gctagacaac tcctctatca
     3181 gctttctcaa tgtgacaatc cctataactg tcctcacgga cgtcctgttt tggtgcattt
     3241 taccaagtcg gatatggaaa agatgttccg acgtattcag gaaaatcaca ccagtctccg
     3301 tgagttgggg aaatattaaa agtataaaaa agtctgggaa aaattttcaa aatcagaaaa
     3361 acgcataaaa tcaggtgttc aaaaaccttg attttatgcg ttttatcata gaaatattta
     3421 cttcattttt tcctaattct tttcgaaact ctttttaaac gacgtcagtt ttatcagtaa
     3481 tctcaaaaca gtgttttgag ctaattttgc cagttttgtc tgtaacatcg aagttgtgtt
     3541 ttaccactct gcgactggtt tcctagtttg ctctatgatt ttcacagagt attaaattgc
     3601 gattttgcca agtttcttta ttcgtctaaa agtagagtct gttctatgcg tctaatgtac
     3661 gaattaggtt gaccatttca atagctcctt gtgcacactc agaaccctta tttcctgctt
     3721 tagtaccagc tcgttctatg gcttgttcaa ttgtatctgt cgttagcaca ccaaacataa
     3781 caggaatttc gctatttaaa ctgatttggg cgattccctt agatacctcg ctacatacat
     3841 aatcataatg acttgtattc cctctaatga cagctcccaa gcagataatt gcatcatatt
     3901 ttttactttt tgccattttt gatgcaatca gtggtatttc aaaagctcct ggaacccagg
     3961 ctacctctat atctttctcg tttacattct ctcttttgag attatctagt gctccagata
     4021 ataattttga agttataaat tcattaaatc tcgctacaac aatacctatt ttaatattgt
     4081 ttgctactaa attaccttca taagtgttca tttatttttc ctccatattt aaaatgtgac
     4141 ccattcgatt tttctttgtt tctaaataaa aactatcgta aggattggct tctatttcga
     4201 ttgatattct actggaaatg gtaattccat atttttctaa ctgttcaacc ttgtcaggat
     4261 tatttgtcag taaatgaagt gactgaagtc ccagatcttt aagcatttgt gctccaatat
     4321 gatattctct taaatcacct tcaaagccta atgcaagatt ggcatcaagc gtatccatgc
     4381 cttgatcttg taaatgatag gcttttaatt tattgataag tccaattcct cgtccctcct
     4441 gtcgcaagta aagtaagaca cccgaaccat tctcaacaat tattttcata gctttatcga
     4501 attgctgtcc acaatcgcaa cgtaaagagc ctaaaacatc tcctgttaaa cattcggagt
     4561 ggacccgaca taatacattg gcttcatcct ctatgtttcc cataataaga gcaagatgat
     4621 gttccccatt tagtttatct atatagctaa ttgctttgaa attaccgtat ctagtaggca
     4681 tattgacagt tgaaactcgt tctaccagct gatcatatgc ttttctatat tcttgtaatt
     4741 ctttgatggt aattagtgga atgttgtgtt tcttcgagaa ctgaattaaa tcatctattc
     4801 tcatcatttt gccatcatga ttcattattt cacaacatag gccacactct tttagtccag
     4861 ctaattttaa taaatcaaca gttgcttctg tgtgtccatt tctttctaga acaccacctt
     4921 tttttgcaat taaaggaaac atgtgtcctg gcctgcgaaa atcagagggt gttatatctt
     4981 cagctacaca catacgtgcg gtcagtcctc tctcctcggc agaaatacct gtggtcgttt
     5041 ctttataatc aattgaaact gtaaaagcag tcttatgatt atctgtattg ttttcaacca
     5101 taggtgaaag cattaattga ttagctaaac tttcgctcat aggcatacaa attaatcctt
     5161 tggcataagt agccataaaa ttaacatttt ctgttgtagc tgcttgtgca gaacaaatta
     5221 agtctccttc attttctcta tccttgtcgt ctataacaag aacaagtcgt cccttctgca
     5281 atgcttctaa tgcttcttgt atttttcgat attccattga ctgattatcc tttctgctaa
     5341 aatccatttt gatataatag ttccttcgat atttctgatt ttggagagtt atccatcagt
     5401 ttttgcacat atttacctaa gatatcattt tcaagattta ctgtactccc gacttgttta
     5461 ctcttaagaa tggtttgttt caaggtatga gggataacag atactgaaaa gtttactttg
     5521 gagactttag cgacagtcag actaatgccg tcaattgtaa tagatccttt ttcaactatt
     5581 aaatctaaaa tttctttttg tgtgttgatt tgataccata cagcattatc atcttttttt
     5641 attgacgaga tttttcctgt accatcaatg tgtcctgtaa cgacgtgacc cccaagtcga
     5701 ccgttgacag ataaggctct ttctagattc acctcacttc catgttttaa tagagtaaga
     5761 gctgttcgac tccatgtttc attcattaca tcaactgtaa aggattgatg attgaaatga
     5821 gtaactgtaa gacagatacc atttactgct atactatcgc ctaaatggat atccgttaat
     5881 atttttgagg ctttaattga tagtttacaa ttacgagagt ctttctgtat tctttcaact
     5941 tttccgattt cttcaattat tcctgtgaac atggataaat cacttcactt tctatgagat
     6001 agtcatttcc tatttgagaa aaagcataag gtttcaatct aatagcgtca tttggcaaag
     6061 aaatgccttc acctccgaca ggaaacttgg cactgcctcc aaaaattttt ggtgcaatat
     6121 atattttcag ctcatcaaca atttgttgtt ccaaagcact ccaattcatt agactgcccc
     6181 cttctagaac taggctatca atctgcatgt ttcctagatg ttgcattaaa ctcgataagt
     6241 ctatatgatt gccttttttc tttatggaaa gtatttcaca gccatgattt tgatatagct
     6301 tcattttatt tttgtcttca gaggaagtgg caatgtaagt tttaatatca tttgctgttt
     6361 ttacgatttt agaggtaaga ggagttcgta aatgtgtatc gcatatgata cggataggat
     6421 ttttcccttc ctccaatcta catgtcagca aaggatcgtc ttgaataaca gtattgactc
     6481 ccaccataat tgcactaaca tggtgtcgta actgatgcac atgctttctt gcctcttctt
     6541 cagtaatcca tttggattga tttgttttag tggctatttt tccatcaatt gtcattgcat
     6601 atttcataaa aacatagggt acatgctggg taatatactt tctaaaactt tttattaagt
     6661 taagacactc attttctaaa attccaacag taacttgaag attattttcc tcaagtatct
     6721 ttactccttt tccagataca ataggattac agtctaggct tccaatgact actcttgtaa
     6781 taccactatc gattatagca tctatacaag gaggtgtttt cccgaagtga caacagggtt
     6841 caagtgttac ataaagcgtc gctccgacag gggattctct acagttttta agagcatttc
     6901 tctcagcatg tgagccacca aaaaactcat gataaccttg tccgatgatg tgattatctt
     6961 ttacaataac tgcgccgacc ataggattgg gattgacgta accagcccct ttttgtgcca
     7021 gttttattgc taatttcata tattttgaat cgctcatctc actacctcca aaaaaatata
     7081 ccttgaatag gagactactc aaggcataca aaagaaaact tatgcgatta acaaaaatgc
     7141 tctgaaatga caagtaatca tttcagagca cgcaaaaagc acaaatatac ttttatcttc
     7201 tttcatccag actatactgt cggctttgga atttcaccaa atcatgcctt tcggctcgtg
     7261 ggctatacca ccggtaggga attgcaccct gccctgaaga tagttattca attacagatg
     7321 attatagtac ttaattttga atatgtcaac agataaatac cgattgtttt tgatatactg
     7381 tatttgtgat aatcgattct cgctcctcgg ataaagaaaa tatgatatac tagataaacg
     7441 aaataagaga gaaggaacac tatgtacgca tatttaaaag gaatcattac caaaattact
     7501 gccaaataca ttgttcttga aaccaatggt attggttata tcctgcatgt ggccaatcct
     7561 tatgcctatt caggtcaggt taatcaggag gatcagattt atgtgcatca ggttgtgcgt
     7621 gaggacgccc atttgcttta tggatttcgc tcagaggatg agaaaaagct ctttcttagt
     7681 ctgatttcgg tctctgggat tggtcctgta tcagctcttg ctattatcgc tgctgatgac
     7741 aatgctggct tggttcaagc cattgaaacc aagaacatca cctacttgac caagttccct
     7801 aaaattggca agaaaacagc ccagcagatg gtgctggact tggaaggcaa ggtagtagtt
     7861 gcaggagatg accttcctgc caagatcgca gtgcaagcaa gtgctgaaaa ccaagaattg
     7921 gaagaagcta tggaagccat gttggctctg ggctacaagg caacagagct caagaaaatc
     7981 aagaaattct ttgaaggaac gacagataca gctgagaact atatcaagtc ggcccttaaa
     8041 atgttggtca aataggagca gagaatgaca aaacgttgtt cgtgggtcaa gatgaccaac
     8101 ccactctaca tcgcctatca tgatgaggag tggggccagc ccctccatga tgaccaagcg
     8161 ttgtttgagt tgttgtgtat ggaaacctat caggcaggcc tgtcttggga aacggtactc
     8221 aacaaacgcc aagctttccg agaagccttt catagctatc aaattcactc agtcgcagag
     8281 atgactgaca ctgaattgga agccatgctg gagaatccag ctatcattcg aaatagagcc
     8341 aagatttttg ctacacgcgc taacgcccaa gcctttctac agttacaggc agagtacggc
     8401 tcttttgatg cctatctttg gtcttttgtt gaggggaaaa ctgtcgttaa cgatgttcct
     8461 gattatcgcc aagccccagc taaaacaccc ttatctgaga aattagccaa agatctcaaa
     8521 aaacgagact tcaagttcac aggcccagtc gccgtattgt cttttctaca ggctgcaggg
     8581 ctagttgatg accacgagaa tgattgtgag tggaaaggtc ttaaatgatg tctaacaaaa
     8641 ataagggaat tctgattttt gcgattctct atacagtcct ctttgtgttt gatggcgtta
     8701 aattgctggc ttctttaatg ccatctgcca ttgcaaatta tcttgtttat gtagttttag
     8761 ctttatatgg ctccttcttg ttcaaggata gattgatcca acaatggaag gagattagaa
     8821 agactaaaag aaaattcttc tttggagtct taacaggatg gctctttctc attctgatga
     8881 ctgttgtctt tgaatttgta tcagagatgt tgaagcagtt tgtgggacta gatggacaag
     8941 gtctaaatca gtctaatatt caaagtacct ttcaagaaca accactactg atagctgttt
     9001 ttgcttgtgt cattggacct ctggtagaag aattattttt ccgtcaggtc ttattgcatt
     9061 acttgcagga acggttgtca ggtttactaa gcattattct ggtaggactt gtttttgctc
     9121 tgactcatat gcacagtttg gctctatcag agtggattgg tgcagttggt tacttaggtg
     9181 gaggccttgc cttttctatt atttatgtga aagaaaaaga gaatatctac tatcccctac
     9241 ttgttcacat gttaagcaac agcctctcct taatcatttt agctatcagt atagtaaaat
     9301 gaaatgagaa caggacaaat cgatttctaa caatgtttta gaagtagagg tgtactattc
     9361 tagtttcaat atactgtaat atgtgatgaa aatgccagta atgataccga gaaaaaagct
     9421 gagaaacttt tcccagcttt atttgttata gtcaaagaga atgacttgtt cctgtgcatc
     9481 tacatgagca tggaccccaa agggtacaat tgctcttgga gttgcgtggc cgacattcag
     9541 attatagaca atcgggatat tgctgtcaat gatatccaat agtgcctctt tatagtcgtc
     9601 atggaaagtt tcatccatag gttttccgac caagagtcca ttgatgaccg cgaatatgcc
     9661 agtgtccttt aaagttagca acatcttttt gaagtcttct ggcttaggct tttcttcgct
     9721 tgtttcgagc aagaggattt tcccttccca gtctgacaag tcagggaaaa gtttgtattt
     9781 ttggcagagt tccgtgctat ctgcgtatcg agagttgtca aagatatcgt agagggattc
     9841 gaggcaacca ccgaggattt tcccctcgaa ctgagcactt ccttgcaaca agtcaaaacc
     9901 tgtatttgta tgactgacac gaggtgttcc cagggccgtg ggactaaaat cagttcgttc
     9961 ctcataccaa acgtcactag ggcggatttc tgaaattctt cccgtctcaa tcaattcttt
    10021 aaagtagtga aggctatagg ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa
    10081 ggattgacca taaaaagtct tgattcctaa tttatgcaac atgaggtggt tcatggttgt
    10141 atccgagaag ccaagaaaaa ttttttgttt gataaccttt tggagttggt cattttcaaa
    10201 aagataaggt agcaagcgat aggtatcgtc tccaccgatg gcacatagga tcatgtcgat
    10261 gctatcatca gaaaaggcat gaatcaaatc ctctgcacga gcttcaggat ggtccttgat
    10321 aaagtctaat ccttttaacg aatggggcaa aaagatggga ttgagtccca gatccttgag
    10381 acgttggata cccaagtcca cttcgtgttt gacaaaatcc tctccgataa tgccactaga
    10441 taaactaaca ataccaatag tagaaaccat atctcatcct cctagaaata gattgagcct
    10501 attttatcac aaaatataag agaaagctat gtgggatttc agaagaatac ttttaatact
    10561 cttcgaaaat ctcttcaaac cacgtcagtt ttatctgcaa cttcaaagct gtgctttgag
    10621 caacctgcag ctagcttcct agtttgctct ttgattttca ttgagtacaa ctcaaaaaag
    10681 taagtgaaaa agcaaccgca cctgcagttg cttttatcgt tttcttattc ttgatagcgt
    10741 gttggtcctg cacttgcact acggatattc aagcgtttct tgagatagaa gcgaagggct
    10801 aggagggctg ctccaataat agctagtggc aatggatcca gtactgggtt aaggctagct
    10861 ggtaggaagc ttgttgcaaa gaagacaagc aaccaaagga acatagaagc taggataact
    10921 agtacagatt tccagaaagg tggacgttga ctgcgatcca tatctggtcc atagtattgg
    10981 taaacaaagt agtacatcaa gtagaaggca aatccaccaa ccagtccaac taatagaaga
    11041 gtaatcaatc catagccgaa agcttgatct gccgcaaaga aggttgtgag ggcgctgaca
    11101 agggcaaaga ggctagtgat gaaaagagct gagtccataa tcatcagttt tggatcatca
    11161 ttttcttttg gatgctcttt ttcgtactgc tctttgacag tgaagctatg agcccaatga
    11221 gttggtgcgc cgtataggga acgggcagtt gtacctttag attgctcctc aaggatttgg
    11281 ggaatgactt cctcaaaaat agtctggatt tcagcgtctg ttttcccatc tttgatgaat
    11341 tgttgggtag caatgtggac aaactcttgg tttttcttag ttaatttttg tagatcaatt
    11401 tgagacatag gaactcctct tagaaccatt ttttatggat gagatagaga gtgagcgaga
    11461 cactcatagc aaaggcgata aagacgatta accagaaggc atttggctct ccgtttaggg
    11521 gaatttcatt atccttaaag ttcatcccgt aggcagaaaa gaccatggtt gggatggaca
    11581 tgacgatggt cacaagggcc aaggttttca tgatgttgtt ctggttgtta gaaatgatag
    11641 aggcaaaggt ctctgtcata gaatgcaaga cgtttccata aatatctgcc atctcgatgg
    11701 cctgttgggt ttcaatcagg gtgtcttcaa gcaggtcttc gtcctcaagg tatttcttga
    11761 tattgctggt tgaactggtc aatttcttaa tcacgcgctc atttgttttg agggaggcct
    11821 tgaaatagac gatagttttt tccaattcca tgagctcaat caattcttca ttacgagttg
    11881 attgatgcag ttgactttcg atttgttcac tcttgcggtc gattgaacga agggctgtta
    11941 ggtaaagctc tgcattgcga taaagaattt gaaagataaa acgtgaacgc atgaaggtat
    12001 agaaattacg caatcgacgg ttgataaaga catcaaggac aggtagtggt tccaaacacg
    12061 tagtgataat ggtttcctca gtgatgataa taccaagcgg gatggttacg tagtaggtgc
    12121 ggttatttct ttcctccgtg accggcacgt ctacgataat cagggtatac tcgtcttcaa
    12181 tggtaatacg agacatttct tccgcatcga gcggtgctcg aaggtcggca atatcaatat
    12241 cgaaggtgtt ggcgatttcg agtgattcat tttgagtcgg attgacgaga ttgatccaag
    12301 tacccggttc aagcgtatcg atctctttaa attcagttgt tgtagagaga aaaacttgtt
    12361 tcatatccct tatcctttct catttttcag attttttcac actgtactat tatactacaa
    12421 aatcggcttt tttcaaataa aatcttacaa agacaccgtt gttattggat ttttactttg
    12481 tcttgaaatg ttgattttta aatttgctcc cctttttgct ccccctatag tgcttttagt
    12541 gcagtttcgt agaatgatac tgcttttttt gcgttctctt ttgagaggtg actatatata
    12601 tccatggtca ttgataaagt ggaatgacct aggcggtact ggagttcctt gtatggtatt
    12661 ccagagttaa gcaagagact agcgtgagtg tgacggaatc cgtgaaatcc aatgttagga
    12721 acatcagcac gcttaaaatg tgtttgcaat cttcttttaa gtcttgaact gctaggatac
    12781 tcatgaatga aatcagaaaa taccacgcgt tcacgttgcc ctatcttcca ggcctcttta
    12841 gtttggcgta gtttgtactg cttcagcatg ctaacagtcg cttgatctat atctatatcc
    12901 cgataactag ccttagactt tgggctattg atttctatat cccgatttag tgttttggta
    12961 atatgcacaa cggcattatc taagtcaata tcagaccagg aaagagctaa agcttcattg
    13021 atacggcaac cagtagccaa taggaactta tagagtgttg tctcatagta gtaacgatat
    13081 ctatgagtat ctaaattatc gagataacca agaaattttt ttagttcttg attttcaaag
    13141 tgctttactt ttttccgctt tgctttctga gtgttacgag gtaaaacaac atcacgcgca
    13201 ggattggacg gtatagcctg catggttacg ccatactgta agatacgttt gtttaaagcg
    13261 tgtatcttgt cataatgtag aaaagccgct ttttcccctc tattagtctt gtcagcaagt
    13321 tcgttgataa ttgactggat aagtggagta gtgagcttat cgagcttgta agccccaaaa
    13381 ataggcaata tatgattatc gattagcttt ttaacattgc cttgagtgtt tggcttaacc
    13441 gtattcttat aactttccaa ccagagagtt gccaattctt gatagttttt tatggtaaca
    13501 gccttttgtc tggtagatcc tgctttttga aaagctattt tttcttgatt ggctttctgc
    13561 ttaacttcct tttgtgtccg acctgttact ttggttttta ctttctttcc tgttacctgg
    13621 tcaactccta gatatacatt agcacgataa actttgctac cgtctttttt tataacttca
    13681 gttattttca tgatgataaa cctttctaaa atacatcagc aggcaagcca ttatcaaaaa
    13741 ggttttatat catgctagga gctacgagaa cccccttatt ttcgttttat ttcttccagt
    13801 aaagaataca tagcattaat gtggttatat ttttgaaatt caggatcttg gttttgtgta
    13861 ataaagtata cgtattgtct accagatttg aatttatcga tagcttgttt ttccactaat
    13921 ttttttaaat attttaaaat tgtatttttg tggattttta gtttttcttc caaagcctct
    13981 cttgttacga aacggtgttc gattattaac tctaatatct ttttctcata ttcgttatca
    14041 atatccagat tatctataag ggaagtatcc cataatgtaa attcaactga gtctaattca
    14101 gttcgtaaat tgggatattt taaatgactc tctttaactg cttccattat tttgggtata
    14161 ccagttcccg ctttttcaca taaatttaaa aatctaaaaa tttcttgtat taagtagttt
    14221 cttggatcag aatgtgcccc actaaaaaag tcctttatgt caattctaag tgttccgcca
    14281 tttgtgaata tatacctgtc gggatatcta attatcatta tccctttttc gcttttatag
    14341 tcgctatgaa taaggctatt aatgaatgcc tcacgaattg caattcgtaa ttttgaaatt
    14401 ccaattctat tcatagaatt ttcttgaatg ttagagttgt catttaaagt tagatataat
    14461 ttttcaataa ctaggtaaaa gaaattaaaa agattatctt ccccccatgt accatcataa
    14521 atcacacgat ctttaaagcg attgttttca gaaaactctt ttagaacata ttctacgtta
    14581 tagtgtggaa taaactcttt tattgaatta tgtgtaccaa aaattaatag tccagcaact
    14641 gttggcttta ttttatcgtc cttgcgatct cttcgtaaag catttatttt tattaaaaat
    14701 tgttcgttat ccaactttga aaaagggtgc tccgcattta tattatcaaa tttttctcta
    14761 tatagcttta ttgtttctgt atctaagtct aaaatagaaa aatcctgaat aagtgtgcta
    14821 tcaaaacttt ccttagcgga atctcttaac atagagttga tttgctcttg tgaacaccta
    14881 aaatcaccag tcccctgacg aaaataggta gatgctatgt tgtcattaag atatataggt
    14941 ttatccttgt agtctacctt gttaacaggg ataatgacaa tctcttttcc ttttatattt
    15001 acgacttcaa cacttt
//
LOCUS       DAVHIT010000049        14109 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVHIT010000049 DAVHIT010000000
VERSION     DAVHIT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.050"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            61..858
                     /locus_tag="VVN16_001326"
     CDS             61..858
                     /locus_tag="VVN16_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU5572710.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1006..1128
                     /locus_tag="VVN16_001327"
     CDS             1006..1128
                     /locus_tag="VVN16_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU5572711.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1150..2190
                     /locus_tag="VVN16_001328"
     CDS             1150..2190
                     /locus_tag="VVN16_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU5572712.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2361..3635
                     /locus_tag="VVN16_001329"
     CDS             2361..3635
                     /locus_tag="VVN16_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU5572713.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3645..4523
                     /gene="truB"
                     /locus_tag="VVN16_001330"
     CDS             3645..4523
                     /gene="truB"
                     /locus_tag="VVN16_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5572714.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4764..5066
                     /locus_tag="VVN16_001331"
     CDS             4764..5066
                     /locus_tag="VVN16_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU5572715.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5174..5812
                     /gene="udk"
                     /locus_tag="VVN16_001332"
     CDS             5174..5812
                     /gene="udk"
                     /locus_tag="VVN16_001332"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU5572716.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5940..7280
                     /locus_tag="VVN16_001333"
     CDS             5940..7280
                     /locus_tag="VVN16_001333"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5572717.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7258..7470
                     /locus_tag="VVN16_001334"
     CDS             7258..7470
                     /locus_tag="VVN16_001334"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU5572718.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7467..8342
                     /locus_tag="VVN16_001335"
     CDS             7467..8342
                     /locus_tag="VVN16_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5572719.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8335..9150
                     /locus_tag="VVN16_001336"
     CDS             8335..9150
                     /locus_tag="VVN16_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU5572720.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9143..9574
                     /locus_tag="VVN16_001337"
     CDS             9143..9574
                     /locus_tag="VVN16_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5572721.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9581..11248
                     /gene="recN"
                     /locus_tag="VVN16_001338"
     CDS             9581..11248
                     /gene="recN"
                     /locus_tag="VVN16_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU5572722.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11250..11978
                     /locus_tag="VVN16_001339"
     CDS             11250..11978
                     /locus_tag="VVN16_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU5572723.1"
                     /translation="MTDYYVIGDVHGKAEMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12057..13880
                     /gene="lepA"
                     /locus_tag="VVN16_001340"
     CDS             12057..13880
                     /gene="lepA"
                     /locus_tag="VVN16_001340"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU5572724.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13961..>14109)
                     /locus_tag="VVN16_001341"
     CDS             complement(13961..>14109)
                     /locus_tag="VVN16_001341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572725.1"
                     /translation="IKEQTRKLAAGYSKHSFEVVDETDEVSNHTYGKATLTWFEEIFE
                     EYKN"
BASE COUNT         4157 a         2577 c         3366 g         4009 t
ORIGIN      
        1 gcttcctaaa acctaaagtt tgtgatataa ttatcaataa aaaagattta ggagtttatt
       61 atggtttctt cggaatttat ctcaaagatt gaatttgctt gcaataagaa agaaagtctt
      121 tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt tcgcaggtgc atttttaacc
      181 ttcagtactg ctgcaggtgc agttggggct gacttgatta ataaaattgc accaggtagt
      241 ggacgcttcc tctttccatt cgtttttgct tggggcttgg cctacattgt ttttttgaat
      301 gccgagttgg tcacttcaaa catgatgttc ttgactgctg gtagtttctt aaaaaaaatc
      361 tcttggagaa aaacagctga gattttacta tactgtacct tgttcaacct tatcggagcc
      421 ttgatagcag ggtggggctt tgctcattcg gcagcctatg cgaatctgac acacgatagt
      481 ttcatctcag gtgttgttga gatgaagtta ggccgctcaa atgaattggt cttgcttgag
      541 gcgattttgg caaatatttt tgtaaatatt gcgattctgt catttatttt ggtcaaagat
      601 ggtggtgcca aactttggct tgtgttgtca gctatttaca tgtttgtatt cttaacaaac
      661 gagcacattg cggcgaactt tgcttctttc gcgattgtga aattcagtgt tgctgcggat
      721 tcaattgcca acttcggtgt tggaaatatg cttcgccact ggggtgtgac tttcatcgga
      781 aactttatcg gaggaggcct cttgatgggt cttccatatg ccttcctcaa taaaaacgaa
      841 gatacttatg tagattaaga aaatgagcac gattgagtcg tgcttttttc attttcaaaa
      901 taaggtaata gctatttctt atatcaaaat atagaaaact gatatttgta aactataact
      961 caaggtgcta caatatcctt aataaaataa tatggaggtc accttatgac ttgtgatttt
     1021 aaatttgaaa ctctacaact acatgctggt caagttgtgg ctccagctac taagtctcgt
     1081 gcagtgccga tttatcaaac aacatttttt gtttttgatg acacgtagga aggtgccgat
     1141 ctgtttgcct tgaggaaacc agggaacatt tatactcgta tcaccaatcc tacaacagct
     1201 gcccttgaag gtggtgttga agcgctagca acagcatcag gtatgactgc agtgacttat
     1261 actattttgg cgattgccca tgctggtgac catgtagtgg ctgcttcgac tatttacggt
     1321 ggaaccttca atcttttgaa agaacccctt cctcgttatg gtatcacaac aacctttttc
     1381 gatattgata atttggagga agtagaagca gctatcaaag acaataccaa gcttgtcttg
     1441 attgaaacct tgggtaaccc cttgattaat attccagacc tggaaaaact ggcagagatt
     1501 gctcataaac atcaaatccc acttgtgtca gacaatactt ttgcaacacc ttatttgatt
     1561 aacgtcttct ctcatggcgt tgacattgcc attcactctg tgactaagtt tatcggtggg
     1621 catggtacaa ctattggagg aataattgtc gatagtggtc gttttgactg gacggcttca
     1681 gggaaattcc ctcaatttgt tgacgagggt ccaagctgcc acaatttgag ctatactcgt
     1741 gatgtgggtg cagcagcctt tattatagct gttcgagttc aattgcttcg tgatacaggt
     1801 gcagccttgt caccattcaa tgctttcctc ttgctacaaa gacttgaaac ctcttcactt
     1861 cgtgtggaac gccatgtaca aaatgctgag acaattgttg attttcttgt caaccatcct
     1921 aaggtagaaa aggtaaatta tccaaaactt gcagatagtc cttatcatgc cttggctgag
     1981 aaatacttgc caaaaggtgt cggttcaatc tttaccttcc acgtcaaagg tggcgaggaa
     2041 gaagcacgca aggtcattga taatttagaa atcttttctg accttgcaaa cgcggcagat
     2101 gctaaatcgc ttgttgtcca tccagcaaca accactcacg gtcaattgtc agaaaaagac
     2161 ctagaagcag caggtgtcac accaaactaa attcgtttgt caatcggtct tgaaaatgta
     2221 gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa tttaaagtaa aagaagataa
     2281 acagtgggct tcgactcact gtttttgatt ttccctcagg catgatataa tggttaaaga
     2341 agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc caactgtggg gaagtcttta
     2401 cagtaaatga gagtcagtat gccgaactct tgtcccaagt gagaacggca gagtttgata
     2461 aggaactaca cgataggatg aagcaggaac tggccttggc tgagcaaaag gccatgaatg
     2521 agcaacagac taaactggct cagaaggatc aagaaattgc gcaattacag agtcagatcc
     2581 aaaactttga tacagaaaaa gaattggcca agaaagaggt tgaacagaca agccatgagg
     2641 ctctcttggc taaggacaag gaagtacagc tcttagaaaa tcagttggct accttgcgtt
     2701 tggagcatga aaatcaacta caaaagaccc tttctgacct agaaaaagaa cgggatcagg
     2761 ttaaaaacca actacttttg caggaaaagg aaaatgaatt atctttggct tctgttaagc
     2821 aaaactacga agcccagctc aaggcagcta gtgaacaagt cgagttttat aagaatttta
     2881 aggctcaaca atctacaaaa gcgattgggg aaagcctaga acagtatgca gagagtgagt
     2941 ttaacaaggt tcgtagtttc gcctttccaa atgcttactt tgagaaggat aacaaggtct
     3001 cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg tgatgaaaat ggagttgaaa
     3061 tcatttctat catgtttgag atgaaaaacg aagcggacgg aacagagaag aagcacaaga
     3121 atgcagattt ttacaaggaa ttggacaagg accgtcggga gaagaactgt gagtatgccg
     3181 ttttggtgac catgcttgag gctgataatg actactttaa cacagggatt gttgacgtca
     3241 gtcacgagta tgaaaaaatg tatgttgttc gtcctcaatt ctttatccaa ttgattggtc
     3301 tcttacgtaa tgcggcgcta aattccctaa aatacaagca ggagttggcc ttggttcgcg
     3361 agcaaaatat tgacattacg cattttgagg aagatttgga tgcctttaag ctagcttttg
     3421 ctaagaacta taattcagct tcgactaact ttggaaaagc tattgatgaa atcgacaagg
     3481 ccatcaaacg catggaagag gttaagaaat tcctgaccac atctgaaaac caactccgtt
     3541 tagctaacaa caaattggaa gatgtctctg ttaaaaaatt gacccggaaa aatccaacaa
     3601 tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc aaaaatgaac ggtattatta
     3661 acttaaaaaa ggaagcagga atgacctcgc atgatgcggt ttttaaactg cgtaagattt
     3721 tgggaaccaa gaaaattggt catggtggaa ccttggatcc ggatgtggtg ggtgttttgc
     3781 cgattgcggt tggcaaggcg acacgcatgg tcgagtttat gcaggacgag ggtaagatct
     3841 atgaggggga aatcactctg ggctattcca cgaagactga ggatgctagt ggggaagtgg
     3901 tcgcagaaac ccctgttttg tctctcttgg atgaaaagct tgttgatgaa gcgattgcta
     3961 gcttgactgg acctattact cagattcccc ctatgtattc ggcagttaag gttaatggtc
     4021 gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga gcgtccagaa cgtcaggtga
     4081 ccatttatca atttgagcga acaagtccga tttcttatga tggccaactt gcccgattca
     4141 cttttcgtgt aaaatgcagt aaagggacgt acatccgtac tttgtcagtt gatttgggtg
     4201 aaaagcttgg ttatgcggct catatgtccc atttgactcg tactagtgct gctggcttac
     4261 aattagaaga cgctcttgcc ttggaggaaa ttgctgaaaa agtagaggct gggcaattag
     4321 attttctcca tcctttagag attgggacag gtgaccttgt caaagttttc ctaactccag
     4381 aagaggctac agaagttcgc tttggtcgtt ttattgagct agaccaaacg gacaaagaac
     4441 tggctgcctt tgaagatgat aaattgttag ccattctaga aaaacggggc aatctctata
     4501 agccaaggaa ggtttttagc tagatcgttt aggaataaaa atcgggtgat agataacaat
     4561 tgcttgataa aaccccatac taatagtaga atggttttgg gaattataat attccaattg
     4621 ttgcgagttg taggtactca aataatctat atagaaattt agaggtgtga aatgaagcaa
     4681 tttaaaattc tttcagataa atatttagag tccattacag gttctgatgg gaacttaggc
     4741 ccaggatttg gtgtgataat tccatgatgc gaaatgagtt tcgagaaagg gtggagcaac
     4801 ttcttcaaca aaaagaaata aatgaaaata gtgagttgag tcacctgttt cgtcttgcta
     4861 tacaaaattt agacagaaat gaaaaatacc aatcggtcat ggccaatttg agtcaagggt
     4921 tgtcacttta cctcatgacg catcattacc aggcacctaa gtctgtcatt gattttggtt
     4981 tatggattgc caaagctcct agccaggaaa gagggagact ggctttcttg cagatgcttg
     5041 ctcaaaccct acagggcttt cgttaaaact gtcgaacatc agagggaaga ctctggtgtt
     5101 ttttgctgat gagactataa attttgtaaa aaatgtgata gaatagacga cggataaaat
     5161 aacggaggat agcatgcaaa atagaccaat cattattgga gtgacaggtg gttctggtgg
     5221 tggtaaaacc agtgtttcaa gagccatttt atcgcatttt cctgatgaaa agatttccat
     5281 gattgagcat gattcatact acaaggatca gtctcatttg acctttgaag agcgtgtcaa
     5341 aaccaactat gaccatcctt ttgcctttga tacagacttg atgatcgagc agattaagga
     5401 attgttggca gggcgtccgg tggacatccc gacctacgac tatacagagc atacacggag
     5461 tagcaagacc tatcgtcagg aacctcaaga tgtctttatc gttgagggta ttttggtctt
     5521 ggaggacaag cgtctgcgcg atttgatgga tatcaagatt tttgtggata cggatgacga
     5581 tgtgcgcatt attcgtcgta tcaagcgtga tatggaggag cgtggccgta gccttgatag
     5641 cgttattaac cagtacttag gtgtggtcaa accaatgtac caccagttta tcgagtcaac
     5701 taagcgttat gctgatatcg tcattcctga aggggttagc aataccgtgg ctatcgacct
     5761 gttgacgacc aagattgcaa agattttgga agaagctcga aacagcaaat aatcacatgt
     5821 ggaggccttg cctccttttt ctatttttcc ctcataatgg tacataaatg gagattttta
     5881 ggcatatttt tggtataata atacccatga aagagcaagt aaatgaatag taggtggaga
     5941 tggaaaagta tttatcggta acaactttga ccaagtatct gaaaatgaaa ttcgataaag
     6001 acccatactt ggaacgggtc tatttaactg gtcaagtttc caactttcgt aaacgaccta
     6061 ctcaccaata tttctcccta aaggatgacc atgcagttat tcaagcgacc atctggtctg
     6121 ggatttatca gaaattaggg tttgacctgg aagaaggaat gaagatcaat gtgattgggc
     6181 gtgtacaggt ctatgaacca agtggtagct actccatcat cattgaaaaa gctgagcctg
     6241 atggggttgg ggcgcttgcg attcagtttg aacaacttaa gaaaaaattg acagaagaag
     6301 gcctgtttca agaacgcttc aagcaagctc tgccccaatt ttctaagaga attggtgtag
     6361 taaccagccg tagtggagcc gttattcgag atattatcac gaccgtcagc aggcgattcc
     6421 caggtgttga catccttctt tatccgacca aggttcaagg tgaaggggct gcggaggaaa
     6481 ttgctagaaa tattgcgcgt gctaatcaac gggacgattt ggatttgctc attattggtc
     6541 gtggaggtgg ttctattgag gatctctggg cctttaacga agaaattgtg gtacgagcta
     6601 tttttgaatc tcgtttgcca gttatctcta gtgtggggca tgagacggat gtgaccttgg
     6661 cagattttgt ggcagatcga cgcgctgcaa cgccaacagc ggcggctgaa ctggcaacac
     6721 ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca ggaaaaacgg atggtaacgg
     6781 cagtccgaaa tgttctatct aagaaacaag aggctttgaa aaaatgcagt cagtctgtta
     6841 tctttagaca acctgagcgc ttgtatgacg gttatttgca acgcttggac caactgcaac
     6901 tgcgtttgaa acaaagtttg cgaactcgga tttctgataa caaacaatta gttcaagcaa
     6961 gaactcatca attagtacaa ttatcacctg ttaccaaaat ccaacgctat caagaccgtt
     7021 taggacagtt ggacaagctc ttaggtagcc aaatggcgtt agtttatgac gccaaggttg
     7081 ctgaggccaa gcgactttcg gaagctttgc tcatgttgga tactagccga atcgtggcgc
     7141 gtggttatgc tattgtcaaa aaagaagagt ccgttgtaga ttcggttgag agtttgaaga
     7201 aaaaagacca agtaacgctt ttgatgcgag atggtcaagt agaattagag gttaaagatg
     7261 tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg aaaccattgt ccaaagtttg
     7321 gaaaatggtg aaattgctct ggaagatgcg attactgcct ttcaaaaggg catggtcttg
     7381 tcaaaagagc tccaagctac gctggacaag gctgaaaaga ccttggtcaa ggtcatgcaa
     7441 gaagacggaa cagaaagtga ttttgaatga aaaagcaaga aaaattagct cttgtcgagt
     7501 cggctttgga agatttttat ggagaccagc agtttgcctc tagtttacgg gagtctgttc
     7561 tctattctat tcatgctggt ggcaagcgta ttcggccttt tctcttgtta gaagttctgg
     7621 aagccttgca ggttaccatc aaacctgctc acgcgcaggt agctactgcc ttagagatga
     7681 ttcatacagg gagcttgatt cacgatgacc ttcctgctat ggatgatgac gattatcgaa
     7741 gagggcggtt aaccaatcac aagaaattcg gtgaagctat ggccattttg gctggagatg
     7801 ccttattctt agactcatat gccttgattg cgcaggcaga tttgccaagt cagattaagg
     7861 tggacttgat tgccaactta tcccttgctt caggtagtct gggtatggtg gcagggcaag
     7921 ttttggatat ggagggcgaa caccagcact tgtctttgga agaacttcag actattcatg
     7981 ccaataagac tgggaagtta ctagcctatc ccttccaagc ggcagctatt atagctgaat
     8041 tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga attgattgga cttgcttttc
     8101 aagtcagaga tgatgtactg gatgtgacag ctagttttga ggaaatcggc aagacacctc
     8161 aaaaggatct gcaggcagaa aaatcaacct atcctgccct gttgggcttg gaagagtcca
     8221 ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa attagaagaa attgcccagc
     8281 agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga aagtttgaga atcaatggct
     8341 aaggaaagag tggatgtact agcttataaa caggggttgt ttgaaacgag agagcaggcc
     8401 aagcgaggtg tgatggctgg cctagtcgta gcagtcctta atggagaacg gtttgacaag
     8461 ccaggagaga aaattccaga tgacaccgaa ttaaaactca agggggagaa actcaagtat
     8521 gtcagccgtg gtggtttgaa actggaaaag gccttgcagg tctttgattt gtcggtggat
     8581 ggcgcgacta cgattgatat cggggcctct actggaggtt ttaccgatgt catgctacag
     8641 aatagtgcca agttggtctt tgcagtcgat gttggtacca atcagttggc ttggaaatta
     8701 cgccaagacc cacgagttgt cagcatggag cagttcaatt tccgctatgc tgaaaagact
     8761 gatttcgagc aggagccgag ctttgccagt attgatgtga gtttcatttc ccttagtctg
     8821 attttgccag ccttgcaccg tgtcttggct gatcaaggtc aggtggtagc acttgtcaaa
     8881 cctcagtttg aggcaggacg tgagcagatt gggaaaaatg gaattattcg agatgctaag
     8941 gttcatcaga atgtccttga atctgtaaca gctatggcag tagaggtagg tttttcagtc
     9001 cttggcttgg acttttctcc catccaaggt ggacatggaa atattgaatt tttagcgtat
     9061 ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg agattaaaga agcagtagag
     9121 agggcgcata gtcaatttaa aaatgaataa aaaagagaga cttgaaaaaa ttagaagatt
     9181 ggtgacagat tatcaaatcg gcacgcaaga agaaattgta gaacatttga aagaagcagg
     9241 tatcactgcc actcaggcga cggtatcccg agatatcaaa gagttaggta ttgtcaaaat
     9301 tcctttgaga gacaacacct atgtctacga attgccaaaa tcaatcgtaa aaagtctgca
     9361 actggctgaa gacaatatcg aatcggctga attgatggat aagatgatca atctccaagt
     9421 tattccagga aatacggctt ttgtaaaagc tcagttaatc gagacttttg cagacaagat
     9481 ttttagctgt ttgactgatg atagctcgat tttagtcatt gccagaagtg gaagtctagc
     9541 agaggaaatc tttgaacaag taaaaaattg gtaggtctat atgttacttg aaatttcgat
     9601 aaaaaacttt gccattattg aggctatttc cctcaatttt gaaaagggga tgactgtcct
     9661 gactggtgaa acgggtgcag ggaagtcgat tatcattgat gccatgaata tgatgttggg
     9721 agctcgtgcg acgacagatg ttattcgtca tggtgcacca aaggcagaga ttgaggggct
     9781 tttctcagtt gagaatagtc gtcttttaca ggaaattttt gatgagcaag gtttagaatt
     9841 aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat ggtcgtagta tcagccgtgt
     9901 aaatggtcag atggttaatc tgtctgtttt gcgagctatt gggcaacatc ttgtagatat
     9961 tcatggtcag catgaccatg aggagttaat gcgtccccaa ctgcatatcc agatgttgga
    10021 tgaatttggt gatgccgctt tttgggactt gaaagaaacc tatcaaacga gttttgatgc
    10081 ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa aaccaacagg aacacaaggc
    10141 acgtatcgaa atgttggaat ttcaaatggc agagattgag gcagcaaact tgcaggctgg
    10201 agaagacttg gctctcaatc aagagcgaga taaactcctc aaccacaaaa atattgcgga
    10261 tacactgacc aatgcctaca gtatgttgga caatgaagat ttttcaagtc tggccaatgt
    10321 tcgttcagct atgaatgaca tggaaagtgt cgaagagtat gatcctgaat accgtgaaat
    10381 ttcaagctct ctgtctgaga cctactatgt tttagaagac attagcaaac gtttggaagc
    10441 tatcattgag gaccttgatt ttgatggcaa tcgcctgatg caggttgaga atcgtttgga
    10501 cctccttcat actattactc gtaagtatgg tgggactgtt gatgatgttt tgctttattt
    10561 tgccaagatt acggaagaat acaatctctt gacaggcaat aatctttcgt ctgaggacat
    10621 ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat ttggcaggtc aacttgcttc
    10681 tgctcgtcat aatttggctc agcaactcga agctgagatt aaacaagaac tgcaagatct
    10741 ttatatggaa aaagcccagt ttcaggttcg ttttagtaag ggaaaattca gtcgtgaggg
    10801 aaataaaatg gttgagtttt atatttcaac caaccctgga gaagacttta aacccttggt
    10861 taaggttgct tctggagggg aattatctcg tctcatgtta gccattaagt ctgccttttc
    10921 acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg gatacgggag tttcaggtcg
    10981 tgttgctcaa gctattgctc agaaaattca taaaattggt cagcatggtc aggttttggc
    11041 tatctcccat ttgccacaag taattgcgat tgcagattat caattcttta ttgagaagat
    11101 tagtaatgac cattcaacgg tttcgactgt tcgtctcttg acggtcgaag agcgagtgga
    11161 ggaagttgcc aagatgttgg caggtgatga tgtgacagaa gcagccctga cgcaagccag
    11221 agaattgttg agaaacaggg agaaataaga tgacagatta ttatgtaatt ggagatgttc
    11281 acggaaaagc tgagatgctg gaagaccttc tcaaaacctg ggatggtcag ccccagttgc
    11341 tctttctagg tgatttgatt gacagaggtg aggatagtca ccgtgttctt gaaatggtca
    11401 aggacttggt ggacaatcaa ggggctatct gtttgtccgg aaaccacgag tatatgtttt
    11461 tgacttggct agatgaccca gaagaaagtt atgaccatta tagtcgcaat ggtggagata
    11521 caaccattaa ctctatccta ggtcgtccct tggatgcacc agttgatgga gtagaagatg
    11581 ccaagcgggt tgccgctgaa gcgtcagact tggtcgaatt cattcgtcaa atgccatttg
    11641 tagtagagac agacaagtat atatttgttc acgcaggtat tgatttgact ttggacgact
    11701 ggcatgaaac aacagattat aaaaaagtat ggcttagaaa accattccac ggagctgaaa
    11761 atcatactgg aaaaaccatt gtcttcggtc atacaccggt ctatggtttg ttacagcaag
    11821 aacgcggtac atctgagctt tggactacag aagatggcaa gattggaatg gatggaggag
    11881 ctgtttatgg tggtgtcctt cacgggattg tctttacaga ccaaggaatg acagaacacc
    11941 actttatcga aaatgacggc tttgtcgctg aagattagta ctcctagcag ggtatggtct
    12001 tgtcaaaata tcaaaaacaa tttataataa atagataccc tgaaaggaag agaatcatga
    12061 acttagaaga attgaaaaaa cgacagggga agatccgtaa cttctctatt atcgcccata
    12121 ttgaccacgg gaaatcaact ctagcagacc gcattttgga aaaaacagag acggtttcaa
    12181 gtcgtgaaat gcaggcccag cttttggata gcatggatct agagcgggaa cgtggaatta
    12241 ctattaagtt gaatgccatc gagttgaatt acactgcaaa agatggggaa acttatattt
    12301 tccacttgat tgacacacca gggcacgttg actttaccta tgaagtttca cgttcgctag
    12361 ctgcctgtga gggtgctatt ttggtggtcg atgcggctca aggaattgag gctcaaactc
    12421 ttgccaacgt ttatctggct ttggataatg atttggaaat catgcctatc attaataaaa
    12481 ttgacctgcc ggctgcagat ccggagcgcg tgcgtacaga gattgaagat gtgattggtt
    12541 tggatgccag tgaagcagtt ttggcttctg ccaaggctgg tattgggatt gaagaaatcc
    12601 tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga tgtgacggcg ccacttaagg
    12661 ccttgatttt cgactctgtt tacgatgctt accgtggggt tatcctccaa gtgcgtgtca
    12721 tggacggagt ggtcaaacct ggtgataaga ttcagctcat gagcaatagt aagacctttg
    12781 atgtggccga agtcggtatt tttacaccaa aagcggttgg tcgtgatttc cttgcgactg
    12841 gtgatgttgg ttacattgcg gcgtctatca agacggttca ggatactcgt gtgggtgata
    12901 ccgttacctt ggcaaccaat cctgcggcag aaccattaca tggttataag cagatgaatc
    12961 ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa gtacaatgac ctgcgtgaag
    13021 cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt tgaaccagaa acatctcagg
    13081 cacttggatt tggtttccgt tgtggatttc ttggacttct ccatatggat gttattcagg
    13141 aacgtttaga gcgtgagttc aatattgacc tcatcatgac agctccgtct gttatttaca
    13201 aagttaattt gaccgacggt gagtctatgg atgtgtctaa cccatctgag tttccagacc
    13261 caactaagat tgcgaccatt gaagagcctt atgtcaaggc gcaaatcatg gtaccacagg
    13321 agttcgtcgg agcagtaatg gagctagctc agcgtaagcg tggggacttt gtgactatgg
    13381 actatattga tgataaccgt gtcaatgtta tctatcaaat tcctcttgct gaaattgtct
    13441 ttgacttctt tgataaactt aagtcttcga cacgtggtta tgcaagcttt gactacgaat
    13501 tatcagaata tcgcccatct aagctggtga aaatggatat ccttctcaat ggagataagg
    13561 tggatgccct cagctttatc gttcacaagg actttgccta cgaacgtggg aaactcatcg
    13621 ttgataaact caagaaaatc atccctcgcc aacaatttga agttccaatc caagcggcta
    13681 ttggacacaa gattgtcgct cgtactgata tcaaggccct tcgtaagaac gtacttgcta
    13741 aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct tgaaaaacaa aaagctggta
    13801 agaaacgcat gaaatccatc ggatcagttg aagttccgca agaagccttc ctcagcgtct
    13861 tgagtatgga tgaagaataa gttatcagaa atgcctttcc ttgttcacag gaaatttata
    13921 taagaatcaa tagtaaagca ggcttagatg gtctgctttt ttagttttta tactcttcga
    13981 aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt atggttactg acttcgtcag
    14041 tttcatctac aacctcaaaa ctgtgttttg agtaacctgc ggctagcttc ctagtttgct
    14101 ctttgattt
//
LOCUS       DAVHIT010000050        13519 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVHIT010000050 DAVHIT010000000
VERSION     DAVHIT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.051"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(90..167)
                     /locus_tag="VVN16_001342"
                     /pseudo
     CDS             complement(90..167)
                     /locus_tag="VVN16_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            238..894
                     /locus_tag="VVN16_001343"
     CDS             238..894
                     /locus_tag="VVN16_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU5572726.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            884..1207
                     /locus_tag="VVN16_001344"
     CDS             884..1207
                     /locus_tag="VVN16_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU5572727.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1311..2141
                     /locus_tag="VVN16_001345"
     CDS             1311..2141
                     /locus_tag="VVN16_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5572728.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2295..3149
                     /locus_tag="VVN16_001346"
     CDS             2295..3149
                     /locus_tag="VVN16_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5572729.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3249..4622
                     /locus_tag="VVN16_001347"
     CDS             3249..4622
                     /locus_tag="VVN16_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5572730.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4615..5676
                     /locus_tag="VVN16_001348"
     CDS             4615..5676
                     /locus_tag="VVN16_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU5572731.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMAKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVLVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5678..6370
                     /locus_tag="VVN16_001349"
     CDS             5678..6370
                     /locus_tag="VVN16_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572732.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6400..6969)
                     /locus_tag="VVN16_001350"
     CDS             complement(6400..6969)
                     /locus_tag="VVN16_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU5572733.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPTPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7104..7718
                     /locus_tag="VVN16_001351"
     CDS             7104..7718
                     /locus_tag="VVN16_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU5572734.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7720..9366
                     /locus_tag="VVN16_001352"
     CDS             7720..9366
                     /locus_tag="VVN16_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5572735.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQVLVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRNFEHQASYSDQRQ
                     SIGIINVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9378..10664
                     /locus_tag="VVN16_001353"
     CDS             9378..10664
                     /locus_tag="VVN16_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572736.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKELLPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10716..11249
                     /locus_tag="VVN16_001354"
     CDS             10716..11249
                     /locus_tag="VVN16_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572737.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11327..11797
                     /gene="nrdI"
                     /locus_tag="VVN16_001355"
     CDS             11327..11797
                     /gene="nrdI"
                     /locus_tag="VVN16_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU5572738.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKRIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12090..13352)
                     /locus_tag="VVN16_001356"
     CDS             complement(12090..13352)
                     /locus_tag="VVN16_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU5572739.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIEKAG"
BASE COUNT         3956 a         2528 c         3103 g         3932 t
ORIGIN      
        1 tcagttctat ccacaacctc aaaacggtgt tttgagctga cttcgtcagt tctatccaca
       61 acctcaaaac ggtgttttga gctgacttca tcagttctat ctgcaacctc aaaacagtgt
      121 tttgagcaac ctgcggctag tttcctagtt tgctctttga ttttcattga gtattattaa
      181 gacaatatga ggagaaagat gaaagaaaaa ggattttagg agggggcgca ggcagccatg
      241 ccaacggctc ttggctatgt cagtatcggc ctggcctgtg ggactatcgg tgcgccctat
      301 gtgacacctg ttgagatggg cttgatgagt ctctttgttt atgctgggag tgcccagttt
      361 gccatgttgg cactgattgt ggttcaagct cctgtggcag ctattgctat gacggttttt
      421 ctaatcaact tgcgtctctt tttgttgagt ttacacgcat cgacttattt ccgtcatacc
      481 agtctttggt acaatatcgg tatgtctagt atcttgacag atgagaccta tggcgttttg
      541 atgggtgaat tggcccatac agacaaggta aatcctatgt ggatgcacgg aaacaatctt
      601 aacagctatg tggcttggtt tgtggggaca gtagtcggaa cggctctggg tggcctgcta
      661 ccaaatccag aaatctttgg cttggatttt gccctggttg ggatgtttat tggtattttt
      721 gcttcgcaat ttcagattat gcaaagacgg attcctgtcc gcaatctgct cattatccta
      781 gcagttgttg cggtgtcctt ctttttgctc ttgacagtgg tgtctcagtc actagctgtt
      841 ctgtttgcga cgctacttgg ttgtagcatg ggggtggttt tagatggtca gtaagtatct
      901 tttattagca gttattttct ctggcttggt gacttggatt ctccgtatga ttcccttcat
      961 cttagtcaag tataagggct tgcctgcaat cgttgagcgt tttttgaagt tcttgcccgt
     1021 ttccattatc tttgccttga ttctttcaag cgtagtgaca ggtaaggttg ggagccttcc
     1081 tcaaattaaa tggctagact tcttagccgt ctttccaaca gcttgggtag cctttcgcta
     1141 ccgcaatcta gtcggaacag ttctctttgg agtggtcttg attgccatct tgcgtttggt
     1201 cttttaaatt acccataaaa aaaacttatc acagagatag atatcatata atggcgtaaa
     1261 tgctcttttt ctgttaagat tataaggtat tctattttgg aggaaatgac atgaaaaaaa
     1321 tcgttaaata ctcatctctt gctgccctag gacttgttgc tgcaggtgtg cttgcggctt
     1381 gctcaggggg tgctaagaaa gaaggagaag cagctagcaa gaaagaaatc atcgttgcaa
     1441 ccaatggatc accaaggcca tttatctatg aagaaaatgg cgaattgact ggttacgaga
     1501 ttgaagtcgt tcgcgctatc tttaaagatt ctgacaaata tgatgtcaag tttgaaaaga
     1561 cagaatggtc aggtgtcttt gctggtcttg acgctgaccg ttacaatatg gctgtcaaca
     1621 atcttagcta cactaaagaa cgtgcggaga aatacctcta tgccgcacca attgcccaaa
     1681 atcctaatgt ccttgtcgtg aagaaagatg actctagtat caagtctctc gatgatatcg
     1741 gtggaaaatc gacggaagtc gttcaagcca ctacatcagc taagcagtta gaagcataca
     1801 atgctgaaca cacggacaac ccaactatcc ttaactatac taaggcagac ttccaacaaa
     1861 tcatggtacg tttgagcgat ggacaatttg actacaagat ttttgataaa atcggtgttg
     1921 aaacagtgat caagaaccaa ggtttggaca acttgaaagt tatcgaactt ccaagcgacc
     1981 aacaaccgta cgtttaccca cttcttgctc aaggtcaaga tgagttgaaa tcatttgtag
     2041 acaaacgcat caaagaactt tataaagatg gaactcttga aaaattgtct aaacaattct
     2101 tcggagacac ttatctaccg gcagaagctg atattaaata atttcttgaa atcatccatt
     2161 ctgagtaagg tgggtggttt ctcagttttt agggatatag ttttaaacta tatatctgac
     2221 tatctaataa caatttgtga aatcaaacaa attgtgagat actagtacgg tattattttt
     2281 aaggagaaag aatcatgaaa atcaaaaaat ggcttggcct agcagccctt gctacagtcg
     2341 caggtttggc tcttgcagct tgcggaaact cagaaaagaa agcagacaat gcaacaacta
     2401 tcaaaatcgc aactgttaac cgtagcggtt ctgaagaaaa acgttgggac aaaatccaag
     2461 aattggttaa aaaagacgga attaccttgg aatttacaga gttcacagac tactcacaac
     2521 caaacaaagc aactgctgat ggcgaagtag atttgaacgc tttccaacat tataacttct
     2581 tgaacaactg gaacaaagaa aacggaaaag accttgtagc gattgcagat acttacatct
     2641 ctccaatccg cctttactca ggtttgaatg gaagtgccaa caagtacact aaagtagaag
     2701 acatcccagc aaacggagaa atcgctgtac cgaatgacgc tacaaacgaa agccgtgcgc
     2761 tttatttgct tcaatcagct ggcttgatta aattggatgt ttctggaact gctcttgcaa
     2821 cagttgccaa catcaaagaa aatccaaaga acttgaaaat cactgaattg gacgctagcc
     2881 aaacagctcg ttcattgtca tcagttgacg ctgccgttgt aaacaatacc ttcgttacag
     2941 aagcaaaatt ggactacaag aaagcacttt tcaaagaaca agctgatgaa aactcaaaac
     3001 aatggtacaa catcattgtt gcaaaaaaag attgggaaac atcacctaag gctgatgcta
     3061 tcaagaaagt aatcgcagct taccacacag atgacgtgaa aaaagttatc gaagaatcat
     3121 cagatggttt ggatcaacca gtttggtaat aagaaacagg gaggtgggag agaaaattcc
     3181 acctcttgct tttgtataga gtatagattg taaagaagac tattcgttca tagaaaggta
     3241 gagagaatat ggtttttcct agcgaacaag aacagattga aaaatttgaa aaggatcatg
     3301 tagcccagca ttattttgag gttttgcgta ccttgatttc taagaaatca gtctttgccc
     3361 agcaggttgg actcaaggaa gtcgcaaatt atctgggtga gattttcaag cgtgttggag
     3421 ctgaagtgga gattgatgag agctatacag cgccctttgt catggcacat ttcaagagtt
     3481 cgcgtccaga tgccaagacc ttgattttct ataaccacta tgacactgtg ccagcggatg
     3541 gggatcaggt ctggacagag gatcctttta cgctttcggt ccgcaatggc ttcatgtatg
     3601 ggcgtggggt tgatgacgac aagggtcata tcacagctcg cttgagtgct ttgagaaaat
     3661 atatgcagca ccatgatgat ttacctgtca atatcagctt tatcatggag ggagcggagg
     3721 aatcggcttc aacagaccta gataagtatt tggaaaagca tgcagacaaa ctccgtgggg
     3781 cggatttgtt ggtctgggaa caagggacca aaaatgcctt ggaacagctg gaaatttctg
     3841 gtggcaataa ggggattgtg acctttgatg ccaaggtaaa aagcgctgat gtggatatcc
     3901 actcgagtta tggtggtgtt gtggaatcag ctccttggta tctcctccaa gccttacagt
     3961 ctcttcgtgc tgcggatggc cgtatcttgg ttgaaggctt gtacgaagaa gtacaagagc
     4021 ccaatgaacg agaaatggcc ttgctagaaa cttatggtca acgaaaccca gaggaagtta
     4081 gtcggattta tggattggag ttgcctctct tacaggagga gcggatggcc tttctaaaac
     4141 gtttcttttt cgagccagcg cttaatatcg aaggaatcca gtctggttat caaggtcagg
     4201 gtgttaagac tattttacct gcagaagcca gtgccaagct agaggttcgt ctggttccgg
     4261 gcctagaacc gcatgatgtt ctggaaaaaa ttcggaaaca gctagacaaa aatggctttg
     4321 ataaggtaga attatactat accttgggag agatgagcta tcgaagcgat atgagcgcac
     4381 cagccattct caatgtgatc gagttggcca agaaattcta tccacagggc gtttcagtct
     4441 tgccgacgac agcggggaca ggacctatgc atacggtctt tgatgcccta gaggtaccaa
     4501 tggttgcatt cggtctagga aatgccaata gccgagacca cggtggagat gaaaatgtgc
     4561 gaatcgctga ttattacacc catatcgaat tagtagagga gctgattaga agctatgagt
     4621 agagatatta tcaagttaga tcagatcgat gtgacttttc accaaaagaa gagaaccatc
     4681 acagcggtta aggatgtgac cattcacatc caagaagggg atatctacgg aatcgttgga
     4741 tattctggag caggaaaatc aacccttgta cgggtgatta atctcttgca aaaaccatct
     4801 gcagggaaaa ttaccattga cgacgatgtg atttttgacg gcaaggtgac cttgacggca
     4861 gagcagttgc gtcgtaaacg tcaagatatc ggaatgattt tccagcattt taacctgatg
     4921 agccaaaaga cagcagagga gaatgtagcc tttgccctta aacactctgg actcagcaag
     4981 gaagaaaaga aggctaaagt agctaagttg ttggacttgg ttggtttggc agatcgtgct
     5041 gaaaactacc cttcacaact atctggaggg caaaaacagc gtgtggcaat tgcgcgtgcc
     5101 ttggccaatg atccaaaaat cttgatttca gacgagtcaa cttctgccct tgatccgaag
     5161 acaaccaagc agattttggc cttgttgcaa gatttgaacc aaaaattagg cttgactgtt
     5221 gtcttgatta cgcatgaaat gcagattgtc aaagacattg ccaaccgtgt ggcggttatg
     5281 caggatgggc atttgattga agagggtagt gtgcttgaaa tcttctcaga ccctaaacaa
     5341 cctttgactc aagactttat ctcaacagcc acaggtattg acgaagccat ggccaaaatc
     5401 gagaagcaag aaatcgtgga acacttgtct gaaaacagtc tcttggtgca actcaagtac
     5461 gctggagctt caacagacga gccacttttg aatgaattgt acaagcatta ccaagtaatg
     5521 gctaatattc tctatgggaa tatcgaaatt ctcgatggta ctcctgttgg agaattggtg
     5581 ttggtcttgt caggtgaaaa agcagcgttg gcaggtgccc aagaagccat tcgtcaagca
     5641 ggtgtacaac taaaagtatt gaagggagta cagtaagatg gaatcattga ttcaaaccta
     5701 tttaccaaat gtctataaaa tgggttgggc tggtcaggca ggctggggaa cggctatcta
     5761 cttaactctt tatatgacag ttctttcctt cattatcgga ggcttcttgg ggctagtggc
     5821 aggtctcttt ctcgtcttga cagcgccagg tggtgtcttg gagaataaag tcgtattctg
     5881 gattttagac aaaattacct caatttttcg tgcggttccc tttatcatcc tcttggcaat
     5941 cttgtcacca ctttctcact tgattgttaa aacaagtatc gggccaaatg cagcccttgt
     6001 cccactttct tttgcagtct ttgccttctt tgcccgtcag gtgcaggttg tcttggctga
     6061 attggatggc ggtgtcattg aggcggctca agcgagcgga gcgactttct gggacatcgt
     6121 gggtgtttac ctatcagaag gtcttccaga tttgatccgt gtgacgactg tgaccttgat
     6181 ttcccttgtt ggggaaacag ctatggccgg tgcggttgga gctggtggta tcggtaacgt
     6241 agccatcgct tatggattta accgctacaa tcacgatgtg accatcttgg caaccatcat
     6301 tatcattttg attatctttg caatccaatt cttgggagat ttcttgacta agaaattgag
     6361 ccataaataa aaaagagccg tgtggctctt tttaactgat cagattttct gtgcaaattt
     6421 tttactcaag gcttgtccaa tcaaggcacc cactagggct ccgatgacaa tacttgcgat
     6481 aaatagaagg acagttccag gggttggagc gaccatgata cggtcgatat attcttggga
     6541 ttttcctctt gccagaagag tagccatata ggctttgggc gcaatccaca taagcaagat
     6601 tggtcctgtt gtactaaagg cgaaaataat gaaagaaagg aagttctttg ttttgtcctt
     6661 gtattttcct aaatgagcta cttcatctgc taggaggcca cagataattc caggaaggaa
     6721 ggaaccagca ccgtgtttag ttcccaagaa aaagaaggca atgacaaggc cgatagtggt
     6781 aatggctcca aagcgcggaa cttttgcgac taggatcata tagacgctac cgccgacaag
     6841 ggcagtaaag gcaggcgcat aaaacatgtt tccagtttgg tcaaagagat tgcccaaaag
     6901 gacaccaatc cccatgcaga ggaagtaaag aactgcaaaa agcagtgtag ttaagataga
     6961 ttttttcatg aattgtctcc tgataatttt ttcacaattc tcattgtacc acggtttggt
     7021 gggattgtaa atggatacta gaagtttttg aaaacgcttg aaatatgata taatggtttc
     7081 atagttattt ttaggggaat atcatgggta gatttttaga ctttgtcttt aatcgtttct
     7141 ttttagggat gattgcgaca gccttctttt ggctattaac tttagcagga gggattatcc
     7201 ttggtctagc gccggctagt gccaccttga tgagcttata tgcagaacat ggttatagct
     7261 ttcgggaata cagtttgaag gaggcttggt ctctttacaa gcaaaatttt gtctcaagca
     7321 acctgatttt ctatagcttt ttaggtgtgg gtctagtttt gacctatggt ttgtatctct
     7381 tggtgcaatt gcctcatcag accattgttc atttgattgc gacccttttg aatgtcctag
     7441 tagttgccct gatctttttg gcttatacag tatctttaaa attacaagtt tattttgcct
     7501 tgtcctatcg aaatagtctc aaattatcct tgattggcat ctttatgagt ctagcagctg
     7561 tggctaaggt tctccttggg actgtgctac ttgtagcaat tggttattat atgcctgccc
     7621 tgctattttt tgtaggaatt gggatgtggc atttctttat cagtgatatg ttggaacctg
     7681 tctatgaaat catccatgaa aaattggcga caaaatagaa tgaagcagtt ttggctacat
     7741 acgcttctaa gaacctatag ttcagtgatg atcattatca ttgcgagttt tgcaatctta
     7801 ctctcttacg ctgactggga ttcacgtgaa aaggaagccc agagagtagc ccagcgtgta
     7861 actgctagaa cagtgagtga aattgaatat taccatagag agtcaaccca gatagctcag
     7921 gttttagttg aaaatcaagc tcgtattgag ggaatctata aatactttag ccttagcatg
     7981 ccagactatt tttactggca attagagcgg aaagcttcgc cttatatatc agtctctctg
     8041 tatgaaaatg ttgatgacct ctatgttcga aatgattttg taactggggt ggccattgct
     8101 tttcaagatt acaaggaagt ctatgtttct actaaagaca aacgtagtgg agaaaaaatc
     8161 agggctgagg atttcaaacc agcaggaaat agttttgcca ttccggtgtc agatccagtg
     8221 tcagatcaag atttaggagt gatttacatc tccttggatc ctgctgtttt ataccatgcc
     8281 attgataata ctagaggtca tactccgatg gcagtaacag tgacctcacc ttttgatacg
     8341 gagatttttc atattggtga gacagttgat agggagagtg aaaattggct agttggctta
     8401 acttctcatg gatatcaggt tcaggtggca gttcctaaaa actttgtttt acaaggaaca
     8461 gtgactagct ctgctttgat tgtgggtttg agccttctct ttattgtcat tctttatctg
     8521 actttgaggc agacttttgc taattaccaa aagcaggtag tggatttagt ggaatccatt
     8581 caagtcattg ctcaaggcga agaggggcgt cggattgaca tttccgaaaa agatcaggaa
     8641 ttactcctaa tcgcggagac gaccaatgat atgttggatc gattggaaaa gaatatccat
     8701 gatatttacc agttagagct tagtcaaaaa gatgccaata tgcgagcctt gcaggcgcaa
     8761 atcaatcctc attttatgta taatacgctg gagttcttgc gcatgtatgc agttatgcag
     8821 agtcaagatg agttggcaga tatcatttat gaattcagta gtctcttgcg taacaatatt
     8881 tccgacgaaa gagagaccct cctcaaacag gaattagaat tttgccgtaa atacagctat
     8941 ctctgcatgg ttcgctatcc caagtccatt gcctatggtt tcaagataga tccagagtta
     9001 gagaatatga agattcccaa gtttaccttg caaccgctgg tagaaaacta tttcgcgcat
     9061 ggtgttgacc acaggcggac agataatgtg attagcatca aggctcttaa acaggatggt
     9121 tttgtggaaa ttttggtggt cgataatggt agaggaatgt cggctgaaaa gctggcaaat
     9181 atccgagaaa aattaagtca gagaaacttt gaacaccaag ccagctacag tgatcaaagg
     9241 cagtctatcg gaattatcaa tgtacacgag cgttttgtgc tctattttgg agaccgctat
     9301 gccattacta tagagtctgc agagcaagcc ggtgttcagt atcgtattac aattcaagat
     9361 gagtagaaag ggagaaaatg tataaagtat tattagtaga tgatgagtac atggtgacag
     9421 aaggtctgaa gcgtttgatt ccctttgata agtgggatat ggaggtcgtc gcaacagcca
     9481 gtcatgccga tgaagctcta gaatatgttc aggaaaatcc tgttgatgtc atcatttccg
     9541 atgtcaatat gccagacaaa acagggcttg atatgattcg ggagatgaaa gagctcttac
     9601 cagatgctgc ctatatcctg ctctcaggtt accaggagtt tgattatgta aaaagagcaa
     9661 tgaaccttag tgtggtggac tatttggtca agcctgttga taaggtagag ctgggaaatc
     9721 tgctggagaa gattgcaggt cagctcggcg agagagggaa gaaaagtcag actcttagtc
     9781 aagaattaga cgaggctgga tttgttagtt atttagggga taaggagaat tggtggatag
     9841 gtctatccaa ggaaaaacaa ggttccttca ccattcccta ctatgtcttg ggtcaagact
     9901 ggcagatttt catttctgac caacccctag atggtttagt cgttacacct tttgaagctc
     9961 cttatcaaga acactttgaa cgctggaagc tgaatgctga gaaaaccctc ttttacggtt
    10021 ctgtaaatct gcagcagtct gagagtctct ttgcctatta cgaaccgatt tatagggtta
    10081 tcattcaggg aaatctcaat caaatcgtag aagagttaaa tctcttggag aaggtagttc
    10141 ttgaaaatac gccgcgtgtt ccgattacta aacagctttt tattcagttt gtcatggatg
    10201 ttttccattt atttgaacat ctcaaagctg atgacctgac agacatcgtc aaaaccattc
    10261 atgctattca atccttcgat gaattggttc cttatatcaa ggaaactctg agcagctttt
    10321 tcggtcaata ccgtatgaat gaaaatgtgg tcagtgtgct ggaagtcatt ggtcgtgatt
    10381 accaaaaaga gctttccctc aaggatatca gtaaggctct ctttatcaat cctgtctatc
    10441 tagggcagtt gattaagcgt gaaaccgatt cgacttttgc agagttacta aacaaacaac
    10501 gtattaaggc tgcccaacaa cttttgcttt caactagtga cagcatcgaa gatatttgtt
    10561 atgctgttgg ttacagtaac cttggatatt tctataaagt tttccgaaaa ttgtgcggaa
    10621 aatcgccaaa agcctaccga aaacaggtag aaactatact ataaaatttg tattccttta
    10681 caaaaggtgc tataatatta tcaggaggtt ctatcatgaa aaagaaacca atttatctat
    10741 gggtcttgct aatcttgtct gcccttattt cagttccgtc tctgtttgga attgtgagtc
    10801 ccttgcctag taaagaagcc cttcgtgctg ctcagaaaca agttgcaggg gtcaatgctc
    10861 agcaattaga agaccagctt aattatacct atagagtagc agaagcatct cattctattt
    10921 ttaatgttgc tttgattgtg ctatctacta ttttagtggt cgtagcgatt gtcttccttg
    10981 ttcgtaaaaa tttgcaatat gcaaattata cttacgttgg ctatgttttg ttagctatta
    11041 ttggttcgat ttatggctat gttggtttac aagacgctgt gcagttggtt caagatgaga
    11101 gcatgcgttt gacagtgagt attggttcaa aagctgttag cattttctat atcgtcatca
    11161 atgttctctt cctagccctt gtcttctata agatgtggcg acaacagaaa gccttggcag
    11221 aggaagagga aacagaagag cttgcctaag tattgacaaa aaagaactat caagatacaa
    11281 tcttggtagt tgttttttga ttatagataa aatcatggag gtacatatga agacaatttc
    11341 tttagtttat attagtttga gtggcaacac tgaaagtttt gtgacgcgct tgaaagacta
    11401 tctcttgtcc cagtacaaaa ggattgaggt tcaaaagatt catatcaagg atttggtcaa
    11461 ggaaggcaaa aatttctatg aaatggacca tccctatgtc gcttttttgc cgacctatct
    11521 cgaaggtggg aatggcgtgg ataacggaga tgttgagatt ttgacgacac cggtgggaga
    11581 ttttattgcc tatggtaaca atgctagtaa gtgttttggt gtggttgggt caggaaatcg
    11641 taactttaat aaccaatact gcctaacagc caagcaatac agtcaacgct ttggcttccc
    11701 tgtattggct gactttgaaa tgcgaggcat gctggaagat atcaaacatg tcgcagctat
    11761 tatcgcagat ttgtatgagt tggaaaagga gaattaatac tcaatgaaaa tcaaagagca
    11821 aactaggaag ctagtcgcag gttgctcaaa gcacagcttt gaggttgcag atagagctga
    11881 cgtggtttga agagattctc gaatagtata aagttcttat aagaggtggt tgaagttgtt
    11941 caactgtttt ttgagtataa acagtcttta agaacgtaaa aaagcataag ttcaggtctt
    12001 caaaaccttg ttcttatgct ttttatcata gaaatatttg ctgaattttc tcttgaaatg
    12061 aaaccttatc ttcatctcgc gcttgtccct taccctgctt tttcgattgc gagtgcgtag
    12121 ataaagaaga tggcgaagcc aaagaggaaa atcaatcccg caatggcaag gtgtttgagc
    12181 caagaaggaa gatttttgtt ttcacgcgtt accaaggcgt aattcaaaag agcaaagaaa
    12241 ggtgttgtca ggaaagatcc aatcatggca aagcggagca tggttgaaac ctgaccagcg
    12301 aagaacttga taatgacgat accgatgata gcagtgatgg tcatccagat gttcaaagat
    12361 ttacgattgt cctctttttg actgattagc agtcggagag attcctgatt aacgcgagaa
    12421 tagccatcga taacagttat aactgttcca aagatacaga ggaaggcaat aaaggtaatc
    12481 aagtaacggg accattcgcc aagaacagag gcatacatgc ccacgaattg agagatgtat
    12541 ttggctgaag cagcttcaac cgcctgccct gtaggatact gaatcagtgc tcccagtgcc
    12601 acaaagaaga cggctaggat agctgttcca atataaccag tgttaaagtc aaacagagcg
    12661 tcttctgtgt taaagttgac ggtctttctc ttttcagctg accaaagtga attgatggct
    12721 gaaatttcaa taggagccgg catccatcct aggagggaga cgatgaaggg cagagctgcc
    12781 atttgccaag gtgtcttctc gacaaaatca gaactgtatt ctggatgctt gaccgctgct
    12841 atgataactg ccagaacagt cgcaatggtt aaggcagaca taatccattt gaccatgccg
    12901 tctaaaagtt tgtagcctcc aaagagtagc atagcccaaa tgattgcaac gagaatgagg
    12961 gaccactgag taatgctaag tccaatcatt gggaaggcac tggcgatgat agctgagcac
    13021 agaatggcaa caccagccgt gttgaccata gccgaaaaga cattgaggat aaagaaaatc
    13081 cagagataga gttttccttt ttcggcataa ccttcaacca aagtctttcc agtatcagct
    13141 gtgtattcag caccaaaacg gaaaaatgga tatttaaaga cattggctaa gatgaccaag
    13201 agaagtagag accaaccgta agaaccgcca gcttgagttg aggatacaat gtgggaacct
    13261 ccaacagcgg cagttgccat taggattcca ggtcccattg cttttagctt gcttgcccag
    13321 gttgattgat ttaaggaaat agcttgcgac atgataaata ctcctttttg aattttcaga
    13381 ataatctgta tatgtaatct atattctaca aaaaattttg gaaaaatgca agaatatttt
    13441 gaaaaaagat agaaaattta caaagaaaat ctgaaataac tgttatgata tagcagattg
    13501 aaactagaat agtacacct
//
LOCUS       DAVHIT010000051        13439 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVHIT010000051 DAVHIT010000000
VERSION     DAVHIT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.052"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..99
                     /locus_tag="VVN16_001357"
                     /pseudo
     CDS             1..99
                     /locus_tag="VVN16_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841947.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
     gene            195..1073
                     /locus_tag="VVN16_001358"
                     /pseudo
     CDS             195..1073
                     /locus_tag="VVN16_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
     gene            1474..2595
                     /gene="mnmA"
                     /locus_tag="VVN16_001359"
     CDS             1474..2595
                     /gene="mnmA"
                     /locus_tag="VVN16_001359"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5572740.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            2736..3191
                     /locus_tag="VVN16_001360"
     CDS             2736..3191
                     /locus_tag="VVN16_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5572741.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            3201..5114
                     /gene="mnmG"
                     /locus_tag="VVN16_001361"
     CDS             3201..5114
                     /gene="mnmG"
                     /locus_tag="VVN16_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU5572742.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(5321..5413)
                     /locus_tag="VVN16_001362"
                     /pseudo
     CDS             complement(5321..5413)
                     /locus_tag="VVN16_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5467..7146)
                     /locus_tag="VVN16_001363"
     CDS             complement(5467..7146)
                     /locus_tag="VVN16_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5572743.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(7148..7381)
                     /locus_tag="VVN16_001364"
     CDS             complement(7148..7381)
                     /locus_tag="VVN16_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU5572744.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            7661..7876
                     /locus_tag="VVN16_001365"
     CDS             7661..7876
                     /locus_tag="VVN16_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572745.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(8199..8351)
                     /gene="cibB"
                     /locus_tag="VVN16_001366"
     CDS             complement(8199..8351)
                     /gene="cibB"
                     /locus_tag="VVN16_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU5572746.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(8353..8538)
                     /gene="cibA"
                     /locus_tag="VVN16_001367"
     CDS             complement(8353..8538)
                     /gene="cibA"
                     /locus_tag="VVN16_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU5572747.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            8716..9399
                     /gene="tsaB"
                     /locus_tag="VVN16_001368"
     CDS             8716..9399
                     /gene="tsaB"
                     /locus_tag="VVN16_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5572748.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            9396..9833
                     /gene="rimI"
                     /locus_tag="VVN16_001369"
     CDS             9396..9833
                     /gene="rimI"
                     /locus_tag="VVN16_001369"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5572749.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            9823..10833
                     /gene="tsaD"
                     /locus_tag="VVN16_001370"
     CDS             9823..10833
                     /gene="tsaD"
                     /locus_tag="VVN16_001370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5572750.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKTFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10873..12179)
                     /locus_tag="VVN16_001371"
                     /pseudo
     CDS             complement(10873..12179)
                     /locus_tag="VVN16_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(12455..12806)
                     /locus_tag="VVN16_001372"
                     /pseudo
     CDS             complement(12455..12806)
                     /locus_tag="VVN16_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12913..13206)
                     /locus_tag="VVN16_001373"
                     /pseudo
     CDS             complement(12913..13206)
                     /locus_tag="VVN16_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3842 a         2719 c         3138 g         3740 t
ORIGIN      
        1 ataaagttgc tgatttagaa aaagaaatta gtaaccttga aatattactt ggaggggctg
       61 attctgaaga tgatactgct gctcttcaaa ataaattagc tactaaaaaa gctgaattgg
      121 aaaaaactca aaaagaatta gatgcagctc ttaatgagtt aggccctgat ggagatgaag
      181 aagaaactcc agcgccggct cctcaaccag agcaaccagc tcctgcacca aaaccagagc
      241 aaccagctcc tgcaccaaaa ccagagcaac cagctccagc accaaaacca gagcaaccag
      301 ctcctgcacc aaaaccagag caaccagctc cagcaccaaa accagagcaa ccagctaagc
      361 cggagaaacc agctgaagag cctactcaac cagaaaaacc agccactcca aaaacaggct
      421 ggaaacaaga aaacggtatg tggtatttct acaatactga tggttcaatg gcaacaggtt
      481 gggtgaaaga tggagatacc tggtactatc ttgaagcatc aggtgctatg aaagcaagcc
      541 aatggttcaa agtatcagat aaatggtact atgtcaacag caatggcgct atggcgacag
      601 gctggctcca atacaatggc tcatggtact acctcaacgc taatggtgat atggcgacag
      661 gctggctcca atacaatggc tcatggtact acctcaacgc taatggtgat atggcgacag
      721 gctggctcca atacaacggt tcatggtact acctcaacgc taatggtgat atggcgacag
      781 gctgggctaa agtcaacggt tcatggtact acctaaacgc taacggtgct atggctacag
      841 gttgggctaa agtcaacggt tcatggtatt acctcaacgc taacggttca atggcaacag
      901 gttgggtgaa agatggagat acctggtact atcttgaagc atcaggtgct atgaaagcaa
      961 gccaatggtt caaagtatca gataaatggt actatgtcaa tggcttaggt gcccttgcag
     1021 tcaacacaac tgtagatggc tataaagtca atgccaatgg tgaatgggtt taagccgatt
     1081 aaattaaatc atgttaagaa catttgacat tttaattttg aaacaaagat aaggttcgat
     1141 tgaatagatt tatgttcgta ttctttaggt acctatctta tgatttcagg aaatgtcatt
     1201 aaaaaaacga ctccttttct ctaacttgaa aaatagatta gagaaaatgg gttgttttat
     1261 ttattatagt tatttgaatg aagataagaa gaaggtatac tcacatcatt cacataatct
     1321 gtatattgac tataagtttt aaaaaacaat ttttaagctc ttccttgtct tctctaacca
     1381 agcgtgttat aatgaatact gctcaagcga ccttcaatcg tgaagcacac acgaccttca
     1441 atcgtgaata aacgaataga tgggagactt accatgagtg ataactctaa aacacgtgtt
     1501 gtcgtgggga tgagtggtgg tgttgattcg tcggtgacgg ctcttttgct caaggagcag
     1561 ggctacgatg tgatcggtat cttcatgaag aactgggatg acacagatga aaacggcgtc
     1621 tgtacggcga ccgaagatta caaggatgtg gttgcggtgg cagaccagat tggcattccc
     1681 tactactctg tcaattttga aaaagagtac tgggaccgcg tttttgagta tttcctagcg
     1741 gaataccgtg cagggcgcac gccaaatccg gacgttatgt gcaacaagga aatcaagttc
     1801 aaggcctttt tggactatgc cataaccttg ggggcagact atgtagcgac tgggcattat
     1861 gctcgagtgg cgcgtgatga ggatggtacc gttcacatgc ttcgtggcgt ggacaatggc
     1921 aaggatcaga cctatttcct cagccaactt tcgcaagaac aacttcaaaa aaccatgttc
     1981 ccactaggac atttggaaaa gcctgaagta cgcagactag cagaagaagc aggcctttcg
     2041 actgctaaga agaaagactc gacagggatt tgctttatcg gagaaaagaa ctttaaaaac
     2101 tttctcagca actacctgcc agctcagcct ggtcgcatga tgactgtgga tggtcgcgat
     2161 atgggcgagc atgcaggtct tatgtactat acaatcggtc agcgtggcgg actcggtatc
     2221 ggtgggcaac acggcggtga caatgcccct tggttcgttg tcggaaaaga tctaagcaag
     2281 aatattctct atgtaggaca aggattctac catgattcgc tcatgtcaac tagcctagaa
     2341 gccagtcaag tccactttac tcgtgaaatg ccagaagagt ttacgctaga atgtacggct
     2401 aaattccgtt accgtcagcc tgactctaag gtgaccgttc atgtcaaagg agaaaagaca
     2461 gaggtcatct ttgcggaacc acaacgcgcg attacaccag gacaggcagt tgtcttttac
     2521 gatggcgaag agtgtctagg tggcggtttg attgacaatg cttaccgcga tggacaagtt
     2581 tgtcagtaca tttagattga caaattttct caatttgcta caataataac agcaatagaa
     2641 atgatggtca aagctcatgg atgttgcagg cttttttgtc ctgcacttct ttgtagtttt
     2701 gactgttttt gtgtcgttta agggaaagga caagaatgac tcaacaagac tttcggacaa
     2761 aagtagacaa tacggttttt ggtgttcggg ctacagcctt gattgtccaa aatcacaagc
     2821 ttctagttac caaagacaag ggcaagtatt acactattgg aggtgcgatt caagtcaatg
     2881 aaagcacgga agacgcggta gtccgtgaag tgaaggaaga actgggtgtc aaagctcaag
     2941 ctgggcagct agcttttgtg gttgaaaatc gttttgaagt ggacggcgtt tcctatcaca
     3001 atatcgagtt tcattatctg gtggatttgc ttgaagatgc cccgttgacc atgcaggaag
     3061 atgagaaaag gcagccctgt gagtggattg acttggataa gctccagaat atccagctag
     3121 ttccagcctt tttaaaaaca gccctaccag attgggaagg ccaactaaga cacattcatc
     3181 ttgaggaata ggagagaaac atgacttatc attttactga agaatacgat attattgtaa
     3241 ttggtgcggg acacgctggg gttgaggctt ccttggccgc tagccgtatg ggctgtaagg
     3301 tcctgcttgc gaccatcaat attgaaatgc tggctttcat gccttgtaat ccctctatcg
     3361 gtggttctgc caaggggatt gtcgtgcgtg aagtcgatgc cctcggtggc gagatggcca
     3421 aaaccattga caagacttac atccagatga agatgctaaa cacagggaag gggccagctg
     3481 tccgtgccct tcgtgcgcag gctgacaagg aactttactc taaggagatg cgcaagacgg
     3541 ttgaaaacca agaaaatctg acccttcgtc aaaccatgat tgatgagatt ttggtggaag
     3601 atggcaaggt tgtcggtgtg cgtacagcca cccatcaaga atatgctgct aaggctgtta
     3661 ttgtgacgac agggactgct ctccgtgggg aaattatcat cggagacctc aagtactcat
     3721 caggtcctaa ccacagcttg gcttctatta acctagctga caatctcaag gaactgggtc
     3781 tcgaaatcgg tcgtttcaag acaggaaccc ctccacgtgt caaggcttct tctatcaatt
     3841 acgatgtgac agaaattcag ccaggagacg aagtgcctaa tcatttctca tacacttcac
     3901 gtgatgagga ttatgtcaag gaccaagtac catgctggtt gacctatacc aatggtacca
     3961 gtcatgagat tatccaaaac aacctccacc gtgcgcctat gtttacaggt gtggtcaagg
     4021 gagtggggcc tcgttactgt ccgtcgattg aagacaagat tgtgcgcttt gcggacaagg
     4081 aacgtcacca actctttctt gagccagaag ggcgcaatac tgaggaagtc tatgtgcaag
     4141 gactttcaac cagtctgcct gaggatgtcc agcgtgactt ggtgcattcc atcaaaggtt
     4201 tggaaaatgc agagatgatg cggacaggtt atgctattga gtatgatatg gtcttgcctc
     4261 atcagttgcg tgcgactttg gaaaccaaga aaatctcagg tctcttcact gctggtcaga
     4321 caaatggaac atcaggttac gaagaggcag caggccaagg gattatcgcg ggtatcaatg
     4381 cggctctgaa aatccaaggc aagcctgaat tgattttgaa gcgcagtgat ggttatatcg
     4441 gggtgatgat cgacgacttg gtgaccaagg gaaccattga accctaccgt ctcttgacca
     4501 gtcgtgctga ataccgtctc attcttcgtc atgacaatgc tgatatgcgc ttgactgaga
     4561 tgggacgcga gattggcctt gtggacgatg aacgctgggc tcgttttgaa atcaagaaaa
     4621 atcaatttga taatgagatg aagcgcctag acagtatcaa actcaagcca gtcaaggaaa
     4681 ccaatgccaa ggttgaggag atgggcttca aacccttgac cgatgcagtg acagccaagg
     4741 aattccttcg ccgtccagaa gtttcttacc aagatgtggt ggccttcatc ggaccagctg
     4801 cagaagactt ggatgacaag attatcgaat tgattgaaac agaaatcaag tatgaaggct
     4861 atatttccaa agccatggac caggttgcca agatgaaacg catggaagaa aaacgcattc
     4921 cggccaatat cgactgggat gacattgatt ctatcgcaac cgaagcccgt cagaagttca
     4981 aactcatcaa tccagaaacc atcggccaag ccagccgtat ttcgggagta aacccagcag
     5041 atatttctat tttgatggtg tatctggaag gtaaaaatcg tagtatttct aaaactcttc
     5101 aaaaatcaaa atgatacgtc gtcggcttct tacgaatgag ttcaaagctt ggctttgatt
     5161 catctccagc ctcccatagt ttcccgaact atgggagcta actcaagtta tgctttcgct
     5221 tagccttcta gtctgatttc gatttttatt gatactcttc gaaaatctct tcaaaccgcg
     5281 tcaacgtcgc cttgccgtag gtatggctac tgacttcgtc agttctatct acaacctcaa
     5341 agcagtgctt tgagcaacct acggctagct tcctagtttg ctctttgatt ttcattgagt
     5401 atgagtttta atctcaaaag aaagcataga gaaaaacagc tccgaggacg gggctgtttt
     5461 cttgctttat tcttcatctg gtgtgaggat catcgggatg atgatcggtt cacgttcggt
     5521 attttcatag aggaaggggc gaatagcgtt gacaatggca ccattgacag attgcacgct
     5581 agcatcctta tttttcagtg cgatacgaat ggcattgaag aggatacgct ggctttggcg
     5641 aatcaagtct ccagactctc tcatgtagac aaagcctcgg ctgaggatat ctgggccaga
     5701 cagaatcatc tgcgatttga agtcaacagt tgcgactgct agaacgacac cgtcttcaga
     5761 tagatcgcga cgatctttga ggacagctgc gccaatttca ccgatacgat ttccatcgac
     5821 atagatatct tgggcgttga aatgacctgc gatacgagct gagtcagcag taagggcaag
     5881 cacatcgcca ttgctcatga taaagatatt gtccttctca acaccagtat ccactgctag
     5941 tccagcgtgg actttttgca tgcggtattc accgtggaca ggcatgaagt attttggctt
     6001 aatcaagcgg agcatgagtt tttgctcttg ctggccaccg tgtccagatg tatggatatt
     6061 gttcacttta ccgtggataa cttcgacacc agcttcagaa atgatgttaa tcagcttgtt
     6121 gacgctagta gtgtttccag ggatgggact agaagagaag ataacggtat cacctggttg
     6181 taattgtact tgacggtggg ttccgttggc gatacgagag agggctgcca taggctcacc
     6241 ctgactacct gtacagagga taagaacttc tcctgcagga taatctttga tttcatttgg
     6301 ctcgataaag gttcccttag gagctttgat gtagccaaga tcgattccgt tgacaatggc
     6361 cttttccata gaacgaccaa agaccgcaat cttgcgtcca gtcttaacag cagcttctgt
     6421 tgcctgctgg agacggaaga tatttgaggc aaaggatgca aagatgatac gtccttcaat
     6481 accttggata atcttcataa tggactgacc aacgactttt tcagagttgg taaaggttgg
     6541 tacttccgca tttgtcgagt cagacaggag acagagcacg ccttcttcac caagcgcagc
     6601 catacgatgc aagtccgcag gttctccaac tggagtaaag tcgaacttaa agtcacccgt
     6661 acagacgatt ttcccttgag gagtatgaat gacaatcccc aaaggctctg gaatagagtg
     6721 agtcgttcta aagaaagttg ccttgagatt tttaaaggtc aactcggtgt tgtggttgat
     6781 ttcgtaaagt ttggcgttgc gcaagaggcc gtgttcttcg agtttcccac ggatcaaagc
     6841 caaggcaagc ggtccagcgt agatagggac atttgcttgc ttgagtagga acggaatccc
     6901 accaatgtgg tcctcgtgtc cgtgtgtgat taaaacagcc ttgacgcggt cgatattgtc
     6961 cacgatgtaa gagtagtcag gaatgacata gtcgatacca agcaagtcat cttctgggaa
     7021 tttaatccca gcatcgacga tgataatctc gtcttggtat tcaattccgt aagtgttttt
     7081 cccgatttct cctagaccac cgatggcaaa aacgccgact tcttcaggtt taagagtgta
     7141 ggccatatta gaactccgta atttcgaagg cgccagtttc tttttcgtaa tcgagcaatt
     7201 tgtcagacaa gagttcgata tactcgatat tgtactctgg gcgattttct tcgacaagtt
     7261 ggcgagcagt gatacggccc tcaagttctg agctggcatc gatgtctagg taaagcgtgc
     7321 gtgttgtttc acggcgtggg ctacgttctt ttgtttcttg ataaaaaact ttgtaaatca
     7381 tatagttcct ttctatttac aggtcagctt attccaaact tttagcagat atttgcttga
     7441 tttcattcca ttttgtgtct agattgacct tgattcgtta caattatttc aattatacca
     7501 caaaatgaaa aattagtaaa agacggaaac gagaaagtta ggggacgatt tctttggtaa
     7561 gcgtttgcaa gaataaagaa aatgtattat gttgataacg gaagtgaaat ttattgcttg
     7621 tcataattcc atatagattt taagtcaagg aggttgccct atgtataact tatttgcttt
     7681 tgctatcgga gctcgcttgg tagcttttat tgtttttgtt gcttgcgttt atgcggggaa
     7741 tcttctattt gccaaactgg aacgacgaca aaccaagttc ttgtggaaaa tgacctttgt
     7801 gactctctac tcgctttttc tcctcctcgt gacctatgtc gtttggtttg tattttactt
     7861 tgaattaaat gcttagatgc cctgcttatg ctggggtttt ttgtttgggg atgtagagat
     7921 agtagattta cgacacgaaa attccacccc atctatcaag atgaggtggt ctttgcattt
     7981 ttctaacatg acatgacaat ggaattttca aagcttatta tttttatggg agtcattgtt
     8041 ctgatcaaaa atagagttga agcataaaaa taaaacaata caaacagata aatcgaatgg
     8101 taaagggatg gtagtaatgt tcaaatcatt gagaatgctg agcaaactgt aagagatgaa
     8161 gacaataaaa tttatccttc taaaaatgtg tttcttatct agcatgactt accaaacttt
     8221 ttacgaaggt cttttccaat ggtataacct gccataaata aagcgatacc aacaccgaca
     8281 ccaaagccac ccttgatttc ttgcaattcc ttattagaaa tttcacgata attgttcaaa
     8341 tctttcatca tattaatctc ctcctaaaaa ataaccagct gccaaaccag aacctataag
     8401 tgctgttcca cctgcaatag ccttccaaga tactgctact ccaaagataa ccaagggagc
     8461 gagaccgcca tcaatatctg ataattcatt ttcagataag gataaaaatt gattgtcaag
     8521 aatgtcaaaa tttgtcattg aactttcctc cttttttctt gttcactttt atattcggaa
     8581 aagaaaagga aaagtgagca tttttttaaa ttatttttag ttaagtgagg gcactcttgt
     8641 ctgggggcaa agaaaattcc catgtgccag cttttgtagt ataatagtaa gcagacaaaa
     8701 agataggaat gtgttatgaa agtattagct tttgatacgt ccagcaaggc tctttctctg
     8761 gctattttag aggataagca ggttcttgcc gagacgacga ttaatattaa gaaaaatcac
     8821 agtattactc ttatgcctgc catcgatttt ttgatggcaa gtttggattg gacacccaag
     8881 gatttggacc gaatcgtggt agctgaaggg ccgggtagct atacaggctt gcgaattgcg
     8941 gtagcaactg ctaagacctt agcttacacc ctgaacattg agttagttgg gatgtcgagt
     9001 ctcttggctc tggtgcccca ccaacaagaa ggtttgtttg tccccttgat ggatgcgcgt
     9061 cgcaataatg tttatgcagg attttatgaa aatgccaaac ctgtcatggc agaagcgcac
     9121 ctatcttttg aagaggtgct agaaaaagtc aagggtacta gtcaggtaac ctttgtcgga
     9181 gaagttggcc cctttgttga gcagattcaa aaacacttgc caaggactga ttacaaagaa
     9241 acattgccca atgcagctaa tctagctctt ttggcctggg acaaggaagc agactccttg
     9301 catgattttg tgccgaatta cctcaaacga gtcgaggctg aggaaaactg gctcaagaac
     9361 cataccgagt ctggcgagtc ttacattaaa cgcctatgat agaaatcaag cgaattcaac
     9421 aacagcctga cctagctcaa gccatctacg ctgttatggc agctgtttac ctagtcagtc
     9481 cttggactct ggagcaaatc caagcagatc tgtcccaaga ccagacttgg tatgcattgg
     9541 cttatgatgg ggcagaagtg attggatttc tagctgtgca ggagaatctt tttgaagcag
     9601 aagtcctgca aatcgctgtc aaaggagctt atcagggtca gggaattgcg tcagccttgt
     9661 ttgctcaatt gccgacagac aaggaaattt tcctcgaagt cagacagtca aatcaacgag
     9721 cgcaagcatt ttacaagaaa gaaaagatgg cagttatcgc tgagcgaaag gcctactacc
     9781 atgacccagt cgaggacgcc attatcatga agagagaaat agatgaagga tagatatatt
     9841 ttagcatttg agacatcctg tgatgagacc agtgtcgccg tcttgaaaaa cgacgatgag
     9901 ctcttgtcca atgtcattgc tagtcaaatt gagagtcaca aacgttttgg tggcgtagtg
     9961 cccgaagtag ccagtcgtca ccatgtcgag gtcattacag cctgtatcga ggaggcattg
    10021 gcagaagcag ggattaccga agaggacgtg acagctgttg cggttaccta tggaccgggc
    10081 ttagtcggag ccttgctagt tggcttgtca gctgccaaga cctttgcttg ggctcacgga
    10141 cttccgctga tccctgttaa tcacatggct gggcacctca tggccgctca gagtgtggag
    10201 cctttggagt ttcctttgtt ggcactcttg gtcagcggtg gccacacaga gttggtctat
    10261 gtttcggagg caggagatta taagattgtt ggggagacac gagacgatgc agttggggag
    10321 gcttatgaca aggtcggccg tgtcatgggc ttgacctatc ctgcaggtcg tgagattgac
    10381 gagctggctc atcaggggca ggatatttat gatttccccc gtgccatgat taaggaagat
    10441 aatctggagt tctccttctc aggtttgaaa tctgccttta tcaatcttta tcacaatgcc
    10501 gagcaaaagg gagaaagcct gtctacagaa gatttgtgtg cttccttcca agcagcagtt
    10561 atggacattc tcatggcaaa aaccaagaag gctttggaga aatatcctgt taaaaccctg
    10621 gttgtggcag gtggtgtggc agccaataaa ggtctcagag aacgcctagc agccgaaatc
    10681 acagatgtca aggttatcat cccccctctg cgactctgcg gagacaatgc aggtatgatt
    10741 gcctatgcca gcgtcagcga gtggaacaaa gaaaacttcg caggctggga cctcaatgcc
    10801 aaaccaagtc ttgcctttga taccatggaa taaagagtgg ctctttgtca agtgtagtgg
    10861 gtaggcgaaa agctacaatc tggagagtac gaaattcgta ctctcttttt tgatgttcaa
    10921 agtcatcaaa atccgttttt taaaattgtt aaagttccgg aaaccaaagg ctttgcgctt
    10981 gataatctta atcaaattat tggtcgcttc aagtttagcg ttggaataag gcagcttaag
    11041 tgcgttgata atcttatctc tatcccttaa aaaagtccaa aagaccgttt gaaaaagcgg
    11101 atgaaccgtt ggcagttctt gctcaatcaa tccaaagaaa tggtcggcat tcttctcttg
    11161 aaaatgaaag agcaggagtt gatagagttc gtagtgaacc tgtaatccct cagagtaaga
    11221 aagcagctta tctcgtactt cccgatgggt taagtgcatt cgaaacgtgg gctgatagaa
    11281 tcgtttctga cagagcttga agctatactg gtgtagtaaa tcagtccagt ggactgattt
    11341 agcccgagcc tagaaacgaa aaagcgaggg ttccaaaagc gcttgagcgc ccgatactcc
    11401 aaggattttc ggtcaaattg gttcatgatt tggattcgta ctcggttcat agctcggctc
    11461 agatgttgga caatgtggaa gcggtcaaga acaatcttcg cctttggaaa gagttgctta
    11521 gcgagccgat aataagggct gtacatgtcc atggtgatga cctcgaccag ctcccgaacc
    11581 tctctctgat agcgttggaa atggtttcga atcaccgcat gagttcgccc gtctaaaatt
    11641 gcgaggatgg atttggactc aaaatcttgg gcaatgaagc tcattttgct ctttttgaag
    11701 ctatactcat cccaactcat aacttttggc aacttggtcc aatcggtttc aaacttaaat
    11761 tccctcagtt ttcggatgac ggtggaagtt gagaccgcca atctgtgggc aatctcagtc
    11821 atagactgct tttcaaggag gagctgagcg attttttgtc tcaccatgtt ggaaatctgg
    11881 caatttttct tgacaatgga tgtttgagaa acgaccacct taaggcaatt cttgcactga
    11941 aagcggcgct ttttgagatg cagtaccgtg ggtaaaccct gacagtcgag aagcggaatt
    12001 ttagaggctt tctggaagtc gtatttgatc atcttccctt gacaatgagg acaaggagga
    12061 gcgggggaat ccaactttgc ctgaacgact agatgggttt ggtatttcag aacagacaag
    12121 attttgatat ttttgtcttc caatccgagc aaatctgtgg tattcttaag ttgttccata
    12181 tgaacccttt ctaatgagtt ggttagacgc ttttcattat aggtcatatg gggctttttt
    12241 tctacaagaa acgaccctat aattcctggg gtgggattac ccactacaga aattatagag
    12301 ccaaagcatt ccaaagtctt gtctgactct ggaatgtttt ctgttcacag ttgttttgta
    12361 actgcctaat aagtaaccgc gctataacga ggtatctacc gagattgtca attctccagt
    12421 atactgttat aaaaaacaaa actggaggat ttttttatgt ccccccatcg tcccatcgac
    12481 tgttccgtag ctgcccaata acggagtata gtcggctgaa acaagaatag gacaaaagtg
    12541 cctcaagaaa agcatcgtaa tttgacaata ttattctgag atgtttttga tgtaagccca
    12601 tattttctca atgggattat actcgggtga ataggtagga agtggtaaca gtctatgccc
    12661 tgctccttac acatgttcat tctgtgaaac cttgcatggt ccataatgat aagggatggt
    12721 gtatctaaag taggtagtaa gaatttttgg aagcaagctt cgaaaaagtc actcgtcatc
    12781 gtatctttat aggtcatcgg agccatagta tattgaaaca aaaaagggca ttagtacttc
    12841 tataacagta tcgcaatctc tggtgtgcgt tgtctcgatc ttaattcaaa taattatatc
    12901 ttcttccaga gagactaact gcctgctcaa ggctcgatca tattcgagat aaaaataggt
    12961 ctcaacccct gtctcataaa tataaatagg agtcaggtag cttaagtgat tcaattcttt
    13021 aaggaaccga tttacttttt ctgggtcttg ttcgcagtag gtacggctct tttttagact
    13081 atatcccata gcctttagag cgtaatgaat agttgttgga ggacagttga attcagcagc
    13141 tatttcagtc aaataagcat cgggatgagt ttcaatatag atctttaatt tatctctatc
    13201 aactttttca attaatagtg gaagttttct taaagttagt gtgttgaaac gtttttatac
    13261 agtgctaaaa tttgaagtgt agtgcagatg tatttgtaat agtattatag caattctttg
    13321 gagaaggtcg agacaagaga aaataggtat ctttgagata taagcatgat ttactataaa
    13381 attattgagt tattcataaa aatttctcta tcaaatatca taatgcgtcg tttaaggag
//
LOCUS       DAVHIT010000052        13216 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVHIT010000052 DAVHIT010000000
VERSION     DAVHIT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.053"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            210..392
                     /locus_tag="VVN16_001374"
     CDS             210..392
                     /locus_tag="VVN16_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5572751.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            337..1186
                     /locus_tag="VVN16_001375"
                     /pseudo
     CDS             337..1186
                     /locus_tag="VVN16_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1238..1600)
                     /locus_tag="VVN16_001376"
     CDS             complement(1238..1600)
                     /locus_tag="VVN16_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572752.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1891..3771)
                     /locus_tag="VVN16_001377"
     CDS             complement(1891..3771)
                     /locus_tag="VVN16_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU5572753.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLATVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3765..4634)
                     /locus_tag="VVN16_001378"
     CDS             complement(3765..4634)
                     /locus_tag="VVN16_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5572754.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4718..7363)
                     /locus_tag="VVN16_001379"
     CDS             complement(4718..7363)
                     /locus_tag="VVN16_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU5572755.1"
                     /translation="MENIVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQAECAKWGKSTQIGYFPDTFGNMGQAPQILQQSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGADGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTNLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLDPQEIRTEFIKKEEI"
     gene            complement(7454..8734)
                     /locus_tag="VVN16_001380"
     CDS             complement(7454..8734)
                     /locus_tag="VVN16_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU5572756.1"
                     /translation="MVYSKEIVREWLDEVAERAKDYPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8906..10990
                     /locus_tag="VVN16_001381"
     CDS             8906..10990
                     /locus_tag="VVN16_001381"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU5572757.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGAWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAT
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11030..12709)
                     /locus_tag="VVN16_001382"
     CDS             complement(11030..12709)
                     /locus_tag="VVN16_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU5572758.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGE
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIHWIGNERGH
                     AGDPLWQKMNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMIIEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3700 a         3133 c         2671 g         3712 t
ORIGIN      
        1 tgttataaaa agttttattc aatcattatc tagtagaata gagtagtaca ttttataaat
       61 tgtcatctct ccataaacgc agtatatcat ttttaaattt taagtcaagg aaaattaact
      121 gagtaaaaac tctcgaaatt caattcgcta tattatcact caacttgaac aaataccaga
      181 accacaaaaa acagatgtaa aggaaaaata tggacttaaa atttgaaggg gttgatttag
      241 aatacaaaaa agctaaaaac aatcttccag agtctttctg ggaaacctac tcagcatttg
      301 caaatacaaa tggcgggaaa ataattctcg ggatagatga aaaaaacatt gacacctacc
      361 aaagagtcaa tagactccct gcgaaattat gaagcaagta aacaattaac tgatacacga
      421 tttaagcgtc ttgttggtgt tcagcacacg acttttaaag agatgttagc tgtattaaaa
      481 acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac
      541 cttcttatgg gcactcttca atatgtgtga gaataccgca cttatgaaga aattgcggct
      601 gattttggta ttcacgaaag caacttactc cgtcggagcc aatgggttga agtaactctt
      661 gttcaaagtg gtgttacgat ttcaagaact cctctcagct ctgaggacac ggtaatgatt
      721 gatgcgacgg aagtacaaat caatcgccct aaaaaaagaa ttagcgaatg attctggtaa
      781 aaagaaatgc cacgctatga aggctcaagc gattgtcaca agtcaaggga gaattgtttc
      841 tttggatatc actgtgaact attgtcatga tatgaagttg ttcaaaatga gttgcagaaa
      901 tatcggacaa gctggtaaaa tcttggctga cagtggttat caagggctca tgaagatatg
      961 tcctcaagca caaacttcac gtaaatccag caaactcaag ccactaacag ctgaagataa
     1021 agcctataac catgcgctat ccaaggagag aagcaaggtt gagaacatct ttgccaaagt
     1081 aaaaacgttt aaaatgtttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg
     1141 aatgaatttg attgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc
     1201 tattgaaata ctaagcctac acagactgat ttcaatttta ccattcttca tagtcaaaac
     1261 catcataatc tggatattgt tctggcgacg ttccaaccga acgtacgaga taagcttctt
     1321 cgtcctctgt ctcgatttct ctcagcactt ggcattcaaa acgccattca tcaccgaagt
     1381 cgaaaataaa cttaaatttt tgtttgacac ttaggctatc cagatagaca ttttctgtgt
     1441 aacgttcttc aacatcgtca ctaacaaagc taagaaagta agaatctgca tgactccact
     1501 caacattatc catgaaaaat gcgtgagcat gatcatttgc aaaatcaaaa ctccataaaa
     1561 tcacatctgc tagttccgct aacgtttttg tttttggaat ctatatatgt cggtagcaac
     1621 cttttttaaa tttgacggaa atgacccaag cttctacaga tgctggcttt tcagcttcta
     1681 tttcttgttt tactaatttt aactgtagca tttgaaacca gataattctt ctgtttcacg
     1741 cgaaacaccc tgcgccaagc aaggtgttct tgtttttata ctcaatgaaa atcaaagagc
     1801 aaactaggaa gctagctgca ggctgctcaa aacactgttt tgaggttgta gatggaagct
     1861 gacgtggttt gaagagattt tcgaagagta ttaagtcgta taaatcgtcg aagctgtcgc
     1921 aatggtatgc cactggttag ctgtagttgc tgcgaaatcc ttgtctgcgt agaagtcagt
     1981 aaatggtagg atttcaactt ccagctcgtc gatgcggtca agctggccag ccagataaac
     2041 ctcgatacgg ctttctgctc gtttgatgcg ttgcaagagt ccgcccatac ggatgtcaac
     2101 tgtatccaaa ccaaagacct tgttttcttt cagccattgg tgactaaaga gggcatggaa
     2161 gtcttcaatt tggcttctaa gttctggtaa ttcttgtctg gcgatttgtt gtaaactttc
     2221 tttatcatcc gcttggtagg cctgacgaat gcgtcgtccc acatctactt tgctacttaa
     2281 aatagcattc aactgggcct gagtttcaaa gagataagca tagtttccag ctttttcttt
     2341 aatgtcagca atagttcccg ccgcctgagc gaagtgtggc ttgtcctgtt caggtgtcat
     2401 gtgtcggtca agtatcggac agagaacatc ctgataaaag acatatcggt tgggattgat
     2461 gccactgaga ttacctggta ggtctggtaa gaggttggca aggtcaatct gcataaaatc
     2521 ttcaaccgtt agaccagtat tggtcttgaa gtgcgcagac aaactatcta ggtcattgcg
     2581 atagctgagt tctgcccaga tttgcaagct tggtaggata gagaactggg cagtttcacc
     2641 accattgtct ccccaacccg ttacgatgac ttctttaatc tgattggcac ggcaggcttt
     2701 attagcctcg atagccacta gacggctaaa atggttgtga ggtgtaaagc caatccactt
     2761 ccaagctccc cctgcaaatg caaggtcatg gctaatcttg tgatgattgc ggaaattacg
     2821 gttgtatttt tcctcgctat cctgataata atcccagtaa accagagtca cacggtcttt
     2881 gagacggtct aggtagacac gagtttcctc tggaatttcc acatcacgat cgtactggcc
     2941 atccgctgac atgagtttga agaacatatc actccacatc tggcagtgga aaccatattt
     3001 gtcagcaata tccagcacgc gctccaagtg ttggcacatg aggagactac gatccacaac
     3061 accgttcaga atcaggtagc gtcccaaacc aaccaagtgg gcttcgtcca tcccgatatt
     3121 gaccttgcga gtcttcagtt tagacaacgt ggcaaacatg ccatcaatca ggtcataaac
     3181 cttttcttcg ccaatgagaa gaatgtcctc tacatcacgg agctcctgca cttccttgac
     3241 accccatttg acaaaggccg acaagtgggc caaggtctgg atgcatggta caaaggtcat
     3301 gtcaaactgt tgagcatagg cttcgatttc ctgcaactcc tctgctgaat aagctccacg
     3361 gaaatagcca aagtaaggct gtccttcaat ctggtaagtg tcttccatgt aaagctcaaa
     3421 ggttgagtag cccatgagag ccaagacctc aatcatctgc ttggcagaag cgacattcag
     3481 caccgcattt cgagaacagt caaccatgta agccaaatct tcgtaagctg cttgttcctc
     3541 aatctctact ttatcagctt ctgctagaac tgttgccaac aaggacaagg cacgataaag
     3601 ttggtgaggt ttgcggtagg tcaattgata gtgaccttcc tcaccctcga tagagataga
     3661 ggcttggtca gactgagtga cagccacttc cacatctggt agagaaatat gaccttttaa
     3721 aacctcaata gcttgcgttt gtttgggact aagtcctgta aatcttacca ttgcttttcc
     3781 tcctgtagcc agttgacaag agcaccgtag agattggcat ctgcgtgata ggtgcaggcc
     3841 tggataactg gtgcgaccgt gtattcttcg taggcatcga caaagtcttc aacagccttc
     3901 ttgacacctt ggataaaatc tggattttga ctgatagagc cacccagact gatgacatct
     3961 ggatcgatga gatactggat attgagcaag ccttgcgcca gattgcggtt cattcgctca
     4021 atggcttctt gacaaagagc attaccagct gcggcctctt ggtaaatctt gcgaccgtcc
     4081 caatcagtat gaccagattt ttcaatcacg tatcgtacca tattcccagt tgatgctagt
     4141 tgcgaccagt tattaagttt ttcagcaggg gcaagggttg tcatgtagcc aaattctcca
     4201 cccagaccgt ggcgacctcg atgaagtcta ccattgataa tcatggctcc gccaatccct
     4261 gtcccaatca cgacacaggc tgcattttca agctctggat gagctagtag ttcactgagt
     4321 ccaacgcagt tggcatcatt ttctaaatgg acaggtagct gataagagct aagcgcctca
     4381 taccaagaaa agccatggat gtagggcacc gcactgaagc catcaatcac acctgtctct
     4441 tgattgaccg cacccggaac gctcatagca atcccactgt aatcctgttc tgacaagcgt
     4501 tgatctagcc acgctagtaa atcctccaag ttttcaggcg ttgaaatact tgtcttatcc
     4561 agtattttcc catcaggagt cagactggca aacttaatcc cagtccctcc gatatcaatc
     4621 gttgcaatgg tcattgtttt taccataaaa aggggcgaat cagcagttag ttcttacttt
     4681 ttcgcccctc ctttcttttg atgtttactt tttgaaatta aatttcttct tttttgatga
     4741 attctgtacg aatctcttgt ggatccaata gtcctgaatg aactggatat ggtcgctcaa
     4801 gtaagtcaag aatggtttgt tgatgttctg atatgcgcac attttcttga ctcatattat
     4861 agtaacggaa gacatagcct tcttcatttt ccgccacttt aaaggctgtt ggacaaactt
     4921 gcggtatgct gagaacagaa tggctcaaga ggctaccagt cgcaaccacg cttccttcct
     4981 gtctagcaag ctgaaggttg gtaaacggtg tctgcaaggc tttggcacga cgataggctg
     5041 aaaagcgttc ttgggcttgg tggcattcga gagcaaactc gacttcaaac tcacgcaaac
     5101 attgtgcttc tggcgttggg aagtagcccc agtcacctag ctcacctgat gcacgcaaaa
     5161 tggtcacggc aatggtgtta tccccaagga tttcgtattc attcaatccc ttgttggata
     5221 cagtcacacc tttttcatcg tcatacagac taacaaaggc ttgttggtgt tgaggatttt
     5281 caggattttc ccatgaagct gctggtttgt ttggtcgtgt caccacctca tagatgcttt
     5341 cagaatcatt gcttggacgc gtgttatgag tcttgaccaa gagacggata cggtgatcct
     5401 tggcagtgtt ggtaaagcga gtcttgaagc ggatttgtgg attgtcaacg aagacagtca
     5461 actcagtttc cagaggaatg tttgtcaatt cttctgaccg tccagcctca cgcttcataa
     5521 actcgatgat accttgttgc tcttcttcta gcttttcatc cgcactgaca ggcacggtca
     5581 attcatgttt gagcaagatt ttagcatagc gatctgtgtt ttccaagacc tcgtggccct
     5641 taagctctgc aaagattggc tctgttcctt ttggttggaa atagatatac tcgtttccga
     5701 tgtcaccccg gtcttcgaag cggataaaat cttcataggc ttcgtgagtt gtcttgtcat
     5761 agactgtgat gttgtcatcc acactcaccg ttacgaatgg tgtatcaatc actccgtttt
     5821 ggtaaatacc ctcacggtgt tcttgttctc cttccagcaa ttggaaggtt gtccaagaaa
     5881 gcggtgctag gtgaactgga atggtcacgc gcacttgtcg agcaatacga gcttggcgga
     5941 acttatcttt tggtaaatcg tactcaaaat tagctccgag gtcttcgatt gtagcctcta
     6001 caggacgacc atccaagtcc tccacacggt aacttggcaa gataagagca gccatctttt
     6061 tgtagccttc tgttgggtgc aattccttga aatcacaagt cgccacatca atcactgtgc
     6121 tgacagtatc gaccttatca tgcaagcctg tgttaatgac agtaaagaga tagtcacttt
     6181 gagccttatc cgtagcaatt ttacccttcc actcgttgag caaattactt ttaacaaagt
     6241 ttcctacttg gttgaccttg gcaaaacgcg tttccatctc gcggtgaact tcgtccacgc
     6301 tacagccaca gatactatca tgtggcgcat tctgcaaaag tgttttccaa gcataggtca
     6361 actgatcctt gtggttgtgt ccaccagtga taatagtcaa gggttctacc acttgctcta
     6421 ggaggttgct attttcttgg aaggcttgtt ttaggtaaat gcgggatgaa gaagtgttgg
     6481 caagtgtgta ccagccatct gtttcctgac tggtcaactc gcctgtaaca gttgataagt
     6541 gttcaggaag cgcaccttct acagcttgaa catattcatc aaaagaacta tgaacaaaga
     6601 ttacatccgg gaagagttca tttgccacac gaatggcttc gctcagattt ttctgtacag
     6661 gctggtggtc acagccgttc atcatcaacc attggttggt cgaagcgtag gcacgcacat
     6721 ctgacaattt ttgtttccag aaggtcaagg cctcatcttt gtcaactggg atttcattcc
     6781 cattactgta ccagttggca aagagaatac ctaaaacacg actaccatcc gcaccctgcc
     6841 agtacatttc tgaaaactgg gaagtaaact gctcatcttc gaggacttgg ttgtcaaatc
     6901 caatcggctt cacaccacga ccaaaggctg ccacgtgaat gcctgattgt tgaagaattt
     6961 gaggcgcttg tcccatattt ccaaaggtat ctgggaaata accaatctga gtagatttgc
     7021 cccattttgc acattctgct tgaccaatca aggtatttcg gacattggct tcactggaga
     7081 tcaagtagtc atcctgcaag atgtaaaagg gaccaatttt aagtttgccc tcgtcaatgt
     7141 agcgttggac cttgtcacga ttttcagggc gaatttgtaa gtagtcgtca aggacaatag
     7201 tttgtccatc caagtggaaa ctcttgaact cagggtcatt ttcaaagaga tcaaagagat
     7261 tgtcaaacaa ttccaccaac tgcatacgat ggctttcaaa aggcaagtac cactcacggt
     7321 cccagtgact atgtgagata atatgtacaa caatattttc catgaagtaa aacctcattc
     7381 taaatttaaa ttttaacgtt ttaaatgtaa acttgtgatt ctgataagaa tcggttgagc
     7441 taaagcgagc cccttagcga atatccaagt aatccaagac caactcacag aacatcatgt
     7501 tagcccaaga gaaccattca cgagagtaga gggtcggatc atctacgtgg aagctttcgt
     7561 gcatgacacc tgtaccacca tcgcaggcaa ccagctgatc cagcaagaat tttttctctg
     7621 ccttatctct tgttgtcaat ccttggatag aaagggcgat tggccagata tagcgataga
     7681 aggtatgaga acttcctaga ccgctagcgt attctccttg gtagaaatat ggattttcag
     7741 agctcaaaat ggtacgacga gtagcttgat acacttcatc atcgaccgaa cagtagccca
     7801 gatagggcgc agctagtaga cttggtacat ttggatcatc catgatgctg gcatttccta
     7861 ggccatccac ttcaaaagcg taaatctttt cgcccttgct gttggtggtg taagcgtagt
     7921 ttttgattcc ttcttggatt tcatcctgaa gacgcttggc atcagcaata acactctggc
     7981 tatcagctag gtttaatgct gcgaagattt cttgcacata acccaagact actacagcaa
     8041 acatatttga cggaatcaag taactatact ggcaacagtc atcactcgga cgaaaagctg
     8101 accaagtcat acctgtcact gcaaagtcag gtccaaagcc atcatttacc aaggtgtctt
     8161 ccttacggtc cgtatctcgg acaaaacgat aaggagagtt cttgtggtct tgttccaccg
     8221 tccacagatg gagaatttcc ttagtcgctg cgacaaaaat ctcatcaaac tgactagtct
     8281 cgccagtctc tttccagagg agataagcca actgcaaagg atagcaaagc gaatccacct
     8341 catacttgcg ctcccagatc cagccgttaa ggtctgtgtg gtcagtctcg tggtgccctt
     8401 tccagttctc ctcaatgttg aaggagttag catagggatc cttgagtacc aaggtcatct
     8461 gacgtttgac caaacctgca atggtctgac gcaggagggc atctctttta gctacatgaa
     8521 ggtagggtct gagttgggct gtcgaatctc gaagccacat ggcaggaata tccccagtca
     8581 agacaaaagt tgaaccatct tctaagattt caaccgtatt gtccaaggta tcggtgtagc
     8641 aacgctcgaa aacatccacc cactctggat agtccttagc ccgctctgct acttcatcta
     8701 gccattctct aacaatttct ttcgaataaa ccattttaaa ttttacctct ttcaaacaag
     8761 tttcatgttc agtatatagc ttttgagaca gaaaatacat acacaaatga tagtcatttt
     8821 tctacaaaat agttatcttt gaaactcctt ttctaaaggc cctctttttc tgatataatg
     8881 tagaaaaaca gctaaaggaa agactatgaa accactactt gaaaccatcg atacccgctt
     8941 tggaactacc aacaagcatg ccttttctcg gggaaatacc ctgccataca caggcgtgcc
     9001 ttttgggatg aattactttg tgccccagac cagtgaccag gatggagctt ggttttttga
     9061 tccgcatctg cctatctttc aggggattcg attaactcac cagcccagtc cttggattgg
     9121 ggactactct tggctccttc tgacacccgt caccagtcaa ctgggtggag acagcctctt
     9181 tcaccgccag tcttcttatg atatagataa agcttgcttc cagcctcact atttgaagct
     9241 cttttctctg cgttatcaga ttgaaaccca gctcacaccg acttgctatg gtgcttctat
     9301 tcgtttgaat caaaagcaag gcaaagccct ctccctctat cttcacgcag cagatgaact
     9361 gacagtagag caagtagata agcggactct tgccctgcga caagaaggta aaactgaaac
     9421 taacaaaaat tcgctaacga tgttcactgc cctgcaaatg aacacggata ttcttgctat
     9481 cagccaagaa gctggagact ggcgaattga cttagcaagt agtcaaaccg agatgcaact
     9541 agccacttct ttcatctctc cttctcaagc tctaatcaat ctacctcaag aagattttga
     9601 tagctgtaaa gcaagtgccc aagcggattg ggaaaatctc ctccatcgtt ttgacattat
     9661 agagacagga gaggctgacc gaaccttctt tgaccactgt ctctacagac tcttcctctt
     9721 cccacagact ttttatgagg ttgatgagtc agggcaagcc atccacatgg atctggctac
     9781 tggtactgtc aagcctggtg tcctctttag caacaatggt ttctgggata ccttccgcac
     9841 caccttcccc ctctttgccc ttatcatacc ggagcactat caacgctttt tagaaggttt
     9901 cctcaatagc taccgcgata ctggtttcct tccaaaatgg ctggctccag atgaacgggg
     9961 tatgatgcca ggtacacttt tagacggtat tatcgcagat agcgcctgca aggacatggc
    10021 ccctgaccta gaaggagaac tcttccaagc catgctcgaa acagccagca aagccgaccc
    10081 tctcggcatc aatggccgcc acggactagc ccaataccaa gaactaggtt acctctctac
    10141 cgaccaccac gaaagtgtta gccatactct agactatgcc tatagtgact tttgtatcgc
    10201 cagctgtgcc aaaaaactag agaacataga aatcgctgaa acctacaagg ctacttcaca
    10261 aaattaccgc cagctatttg acgctgagac aggttacatg cgagcacgag acaatcaagg
    10321 aaactttcac cctgacttct ctccttatag ttgggggcga gactacgctg aatgctctgc
    10381 cattcaagct actttaggcg ttctccacga catccctggc ttaatccaac tgatgggtgg
    10441 aaaagaaacc tttagcaact atcttttgaa agcctgtcaa gatgctcccc tctttgaaac
    10501 aacaggctat ggttacgaaa ttcacgaaat gagcgagatg gctactgctc cttttggaca
    10561 aatagccatt tccaaccagc ctagtttcca cattccttat ctcttccgct acagcgatta
    10621 ccctgactac actgcccttc ttatcaagac gctccgtcag aaagcttttc acccaagttg
    10681 ggaagcctat cctggagatg aagacaatgg tagtctctct gcttggtaca tctggtcagc
    10741 tctcggattt tatccaacct gtccaggaaa accaagctat gacctcggaa tccctctctt
    10801 tgaccatcta cgagtctacc tagctaaaga agataaatgg ctggatatcc atactaaaca
    10861 aaaccacaac cattttaact ttgtcaaaga atgccgactg gacaaaacac tcgtatcaac
    10921 tattcaacac caagacctct taaaagctga acaactaact ttcaccctca gctggttacc
    10981 aagtcactaa taaccaaaag aacctttgca tcgcgcaaag gttcttcttt tatgaaactt
    11041 ggacttgaag ttgatccaca agctgaccat ccaccgttag gttcagatag aaatccaagg
    11101 ttttatctcc tgcgaaatac aaggttggta gcgtcagcct tttttcagtc tcaccagctt
    11161 gaaacgcaag gacttgaatc tcatcctgat aggcgacacc atggacacct gtccccggtt
    11221 gaatcgaaat cttagcctct aaagaggagc tactttcact acgttcaatc ctaaaatgaa
    11281 gagtctctcc ctttgccaca gctaggcttt tttctgcaaa tgctagttcc tgaacaactt
    11341 ctttttttga taatccagga gttttataaa gggaaatctt ggtcaataca ggaatagatt
    11401 gtgcctcgat aatcatcacg cgcacctttt gtgcctctac tagcggacct cgcaagaggc
    11461 gcttatgacc aactgtgaag cccctgccaa attcctgcca gacaccatcc acctctactt
    11521 gcacatgaaa agcagcgatt cgttgcccta gcttcaaatc ttctcttaac tcaattacat
    11581 caaaagtttt aggagaacct aaatcgagtt ctaactggat gggcaagtct gcatcgcttg
    11641 cccaagagct ggtctcaagg ccgtctgtca aatggcgaca agcaaagtct gcggaaagag
    11701 ctggaccaga tacctcagct cccagagcca aatcttcttt atagagctca ttgcgatagg
    11761 tcgcaaattc ataaagtcgt tcaatatcct ttgcatcaaa gagcccagct tgattcggcg
    11821 gaatattaag caagagtgga gttcctcgcc ctactgagtg aaagtagatt tcgaccaact
    11881 cctcgagaga cttaggatcc tgatcctcat ggtagaacca gcctggacgg atggaaacat
    11941 ctgcctctcc gatcgaaaaa atcgtgcccg agggatcccc gtgctgaaga tagttcagct
    12001 ctgcttctgt tcctagttta tcaggattca tcttttgcca cagtggatca cctgcatgcc
    12061 ctcgttcatt gccaatccag tggatactgg tgccttctgt tgaaaaaatc aaacaatcgc
    12121 cctgcaagtc acgaatggtt tcaaaccatt tttcaaattc ataattaacc ttttgcgcgc
    12181 cctctcctct ggcaccatcc atccaaacct cagcgaactt accagcattc ccatagttag
    12241 gatttgataa gatttccttc aactgagcca gataataggc attgtagtcc gcttctcggt
    12301 ccacatgata gaggggacta tgggcatccc acggtgacag gtagaccccc atatccatat
    12361 caaactctgt ggcagcttgg gatacttcaa ggagcaagtc gccctctcct ctcctccaag
    12421 gactgacctt aaccgaataa tctgtgtgag ctgtcggata aaggacaaag ccatcgtggt
    12481 gcttgaccac caaaatcaac tttttgaagc ccgtttcctt gagcacacga acccactcac
    12541 gcgcatccaa ctgactcggg ttaaagcgct caggatcctc ctgtccagtc ccccattctt
    12601 ggtcataaaa ggtattagga ccgaagtgga taaaagctgc tagttcatct cccagataag
    12661 ctagctgagt ctgacttggt aacggtccat gcggtttgat tttcttcatc gttatatatc
    12721 tccttcttta gcaacttctc ttgttcacaa ggttatccac aggttgtgga taataaacca
    12781 tgatctaaca gccagctctt tccaactcca actggtaggc tcaggatttc tctcctcaat
    12841 attccctctt caaacagctc tgtcatatgg ttctgctaac acctctcaga atgctactct
    12901 ccataattat tctcaggctt ctttcaggtc attgtgtgaa gaccattcac tctccagact
    12961 tttccacaga ttgtgaataa gtgattattc taaactatca cctcgtcgac tataacaaaa
    13021 aataaaaaga gggtcaataa gataattatc tcttcctaaa tactcagaaa agattctata
    13081 atttgtacaa ttgaacaaga aatgcttctc caaaatctat cttcaaaaga atgttgcatc
    13141 tttttatcct ttcacacaaa catttctctt tatacagatc caagtgataa ccagatgata
    13201 attggacact ttttgg
//
LOCUS       DAVHIT010000053        13066 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVHIT010000053 DAVHIT010000000
VERSION     DAVHIT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.054"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(41..376)
                     /locus_tag="VVN16_001383"
     CDS             complement(41..376)
                     /locus_tag="VVN16_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEU5572759.1"
                     /translation="MNYDAEGEWTALDDIKNEAYMLKKLADKSYTTNLTDEFYIVDDY
                     FLVQEQVDGLNFEEFIRETEYSLNIREKSLDNIVNIVNDIHKLGYKIVDIAPTNFIYT
                     KKMYCPSNS"
     gene            complement(483..725)
                     /locus_tag="VVN16_001384"
     CDS             complement(483..725)
                     /locus_tag="VVN16_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572760.1"
                     /translation="MHSPVHYRYGAFLKKQAYDEKNKKVIYLLLDEKRKNYVEDKRQN
                     FPSLPSWKMDLFSEEEKRIYFQTTCEVSSKDSAINK"
     gene            complement(926..1102)
                     /locus_tag="VVN16_001385"
     CDS             complement(926..1102)
                     /locus_tag="VVN16_001385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572761.1"
                     /translation="MDLLEKECLKCDKNFQQDDIWNYYHLSDKMPAQGWKIHISSQIK
                     DAVDILRLCINYPN"
     gene            1651..2328
                     /locus_tag="VVN16_001386"
     CDS             1651..2328
                     /locus_tag="VVN16_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU5572762.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            2315..3112
                     /locus_tag="VVN16_001387"
     CDS             2315..3112
                     /locus_tag="VVN16_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU5572763.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            3129..4232
                     /locus_tag="VVN16_001388"
     CDS             3129..4232
                     /locus_tag="VVN16_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5572764.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELILDPLNYPIENYVKRVKSE"
     gene            4533..5552
                     /gene="galE"
                     /locus_tag="VVN16_001389"
     CDS             4533..5552
                     /gene="galE"
                     /locus_tag="VVN16_001389"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5572765.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            5554..6519
                     /locus_tag="VVN16_001390"
     CDS             5554..6519
                     /locus_tag="VVN16_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5572766.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFLYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(6570..6776)
                     /locus_tag="VVN16_001391"
     CDS             complement(6570..6776)
                     /locus_tag="VVN16_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5572767.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            6828..7304
                     /locus_tag="VVN16_001392"
     CDS             6828..7304
                     /locus_tag="VVN16_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU5572768.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            7313..7984
                     /locus_tag="VVN16_001393"
     CDS             7313..7984
                     /locus_tag="VVN16_001393"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU5572769.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLVVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            8115..8453
                     /locus_tag="VVN16_001394"
     CDS             8115..8453
                     /locus_tag="VVN16_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU5572770.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     ELKSEFVRKI"
     gene            8456..9085
                     /locus_tag="VVN16_001395"
     CDS             8456..9085
                     /locus_tag="VVN16_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU5572771.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            9325..10476
                     /locus_tag="VVN16_001396"
     CDS             9325..10476
                     /locus_tag="VVN16_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5572772.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            10788..11537
                     /gene="truA"
                     /locus_tag="VVN16_001397"
     CDS             10788..11537
                     /gene="truA"
                     /locus_tag="VVN16_001397"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5572773.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            11527..12300
                     /locus_tag="VVN16_001398"
     CDS             11527..12300
                     /locus_tag="VVN16_001398"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU5572774.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNVGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            12278..12739
                     /locus_tag="VVN16_001399"
     CDS             12278..12739
                     /locus_tag="VVN16_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5572775.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNDWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            12970..>13066
                     /locus_tag="VVN16_001400"
     CDS             12970..>13066
                     /locus_tag="VVN16_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5572776.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINN"
BASE COUNT         3748 a         2410 c         2873 g         4035 t
ORIGIN      
        1 tgtgcaatac agaactaaat cctttggata aattatttgt ctaactgttt gaggggcagt
       61 acattttttt ggtgtatata aaatttgtag gagctatatc tacaatttta tatcccagtt
      121 tatggatgtc atttactata ttcacaatgt tgtccagtga tttttctcta atatttaagg
      181 agtattctgt ttctcgaata aattcttcaa agtttaaccc gtcaacttgt tcctgaacaa
      241 gaaaataatc atccacgata taaaattcat cagttaaatt agtagtataa cttttatcgg
      301 ctaatttttt tagcatgtaa gcctcatttt ttatatcatc aagtgctgtc cattctcctt
      361 cggcatcata attcacaaaa ggtcttgact gcttgatgat tactttttgc tcgtccgatt
      421 ttctaattgc ccgataaaca tttcctttat ttgatctctt aataattttt tctattttgt
      481 atttatttat ttattgcaga gtccttactt gaaacttcac atgtggtttg aaaataaatc
      541 cttttttctt cttctgaaaa taaatccatt ttccagctag gaagactagg gaaattttgt
      601 ctcttatctt ctacatagtt cttccttttt tcatctagca ataaatagat gactttttta
      661 tttttttcat cataagcttg tttttttaaa aaagccccat atctataatg aactggagaa
      721 tgcattccac attgatagtc agatagtatt tttggagcct taaattctga cagtttattc
      781 gtaagattac aaatcatact cttggcttca gattctgact taggatatag agttataaat
      841 ttgttagcag tagggttcat ttccctaggg gaatgaattt tttttaattc ctctaaattt
      901 ttaacgactt taaagctaca attatttagt ttggatagtt tatacacaat cttaaaatat
      961 ctacagcatc tttgatttga gaacttatgt gtattttcca gccttgtgca ggcatcttat
     1021 ctgacaaatg atagtaattc caaatatcat cttgttggaa atttttatca cattttaaac
     1081 attctttctc taataaatcc ataacaaact cctaattatt aaaataggct agagacacag
     1141 cactagccta ttccgtaagt aaagctactt attttatata aaatataact agcgacctgt
     1201 aattaaacaa gtaaaagaac aatacttagg gagacctgtg acaatgatat cttcctctgg
     1261 aagtagttct aattcaaaca atgtttgttc catgactgta tcctcctgtt atgataaatt
     1321 ccagaaagca tagagcaaat tcgtggttcg tctgactaaa tttcttatct gaacaccacc
     1381 ttcaaataca aattttagtc aaacttttat tttttctaac tttctaatta tattctacca
     1441 tgtgttttta tatgtcaaga attttctgaa taaactgttt attttttatt ttttgacatt
     1501 tttgcaaatt gtttaaaatc tttcaattgg attcacttat tactttagga cattgaaact
     1561 tctttcgcca gaggtgctta tattactggg aagttgaaga ttgaaatgtg gtataataag
     1621 aggtaataga gttttggaaa gtgagagaag atgatttcaa agagattaga attggtagct
     1681 tcctttgtgt cacagggggc tattttacta gatgtgggaa gtgaccatgc ttatctgcct
     1741 atcgagttgg ttgagagagg ccaaatcaaa agcgctattg caggtgaggt ggtggaaggt
     1801 ccctatcagt ctgcggttaa aaatgttgag gctcacggcc taaaggagaa aatccaagtc
     1861 cgtttagcca atggcttggc agcttttgaa gagactgacc aagtgtctgt cattaccatt
     1921 gctggcatgg gtggtcgttt gattgctagg attttagaag aaggtttggg gaagttagct
     1981 aatgtagagc gtttgatcct ccagcccaat aatcgtgaag acgacttgcg tatctggcta
     2041 caggatcatg gattccagat tgtagcagaa agcatcttag aagaagctgg aaagttttat
     2101 gagattttgg tggtggaagc aggacaaatg aagctatcag ccagtgatgt tcgctttggt
     2161 cccttcttgt ccaaagaagt cagtccagta tttgtccaaa aatggcaaaa agaagctgag
     2221 aagctagagt tcgccctcgg acaaatccca gaaaaaaatc tggaagaacg tcaagttcta
     2281 gtagataaga ttcaagctat caaggaggtg ctccatgtta gcaagtgaag tgatccaagc
     2341 ttatgaagcc ttttgccctc aggaattttc tatggaggga gacagctgtg gtctgcaaat
     2401 tggcacttta gacaagggta tccaaagggt catggttgcc ctcgatattc gtgaagagac
     2461 ggtggcagag gccattgaaa agggtgtgga cttgattatc gtcaagcacg cgcctatctt
     2521 tcgtcctatc aaggacttgc ttgccagccg tccgcaaaat cagatttaca tcgacctgat
     2581 taagcatgat atcgcagttt atgtcagcca taccaatatt gatatcgttg aaaatgggct
     2641 caatgactgg ttttgtcaga tgctaggaat cgaggagacg acttatctgc aggaaacagg
     2701 tccagaacgt ggaattggac gtattgggaa tattcagcct cagacatttt gggaattggc
     2761 ccaacaggtc aagcaagtct ttgacctaga tagccttcga atggtgcatt atcaagagaa
     2821 tgatttgcag aagcctattt caagagtagc aatttgtggt ggaagcgggc agtctttcta
     2881 taaggatgct ttggcaaagg gggcagatgt ctatatcact ggtgatatct actaccatac
     2941 tgctcaggat atgctgtctg atggcttgtt agcattggat ccaggtcact atatcgaagt
     3001 gctttttgtg gaaaaaatcg cagcactcct tactcaatgg aaagagaaaa aaggctggga
     3061 actagagatt ttacctagtc aagcatcgac caatcctttc cgtcatatct agttagaaag
     3121 tgaagacaat gaaaaaagtt gccattattg gagcaggaat tgtgggagca acagctgcct
     3181 actacctctc gagagaaagt gacctagagg tgaccgtttt tgaccatgag caaggtcaag
     3241 ccaccaaggc cgcagcagga attatcagtc cttggttttc caaacgccgt aataaagcct
     3301 ggtacaagat ggcgcgcttg ggggctgact tttatgtgga tttattagct gatttagaga
     3361 aatcaggaca aaaaatcgac ttttaccagc gttcgggagt ctttctcttg aaaaaggatg
     3421 aatccaagtt ggaagaactt tatcaactgg ccctccagcg cagagaagaa tctcccttga
     3481 tagggcaatt agccattctg gaccaagctt cagctaatga attattccct ggtttgcagg
     3541 gatttgaccg cctgctctat gcttctggtg gagcgagagt agatggccaa ctcttggtaa
     3601 ctcgtctgct agaagccagt catgtcaagc tggtcaaaga aaaagtgact ctgacactct
     3661 tatcatcagg ctaccagatt ggcgaagagg tgtttgatca ggttattttg gcaacgggag
     3721 cttggttggg ggacatgtta gagcctttag gttatgaagt ggatgtccgt cctcaaaaag
     3781 gacaactacg ggattatcag cttgcccaag acatggaaga ttaccctgtt gtcatgccag
     3841 aaggggagtg ggatttgatt ccttttgcag gtgggaaatt gtccttaggc gctacccacg
     3901 aaaatgacat gggatttgat ttgacggtag atgaaacctt gctccaacaa atggaggagg
     3961 ccaccttgac tcactatctg attttggctg aagctacttc aaaatctgag cgtgtgggaa
     4021 tccgtgccta taccagtgat ttctctccct tctttgggca ggtgccagaa ttggcaggtg
     4081 tctatgcagc cagtggacta ggttcatcag gcctcacaac tggtcctatc attggttacc
     4141 atctagccca actgatccaa gacaaggagt tgatcttgga ccctctaaat tacccaattg
     4201 aaaactatgt caaacgagta aaaagcgaat aatactcaat gaaaatcaaa gaacaaacta
     4261 ggtaactagc cgcgggttac tcaaagcact gctttgaggt tgtggataga actgacgaag
     4321 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt aaccataccc
     4381 atggcaaggc gacgttgacg tggtttgaat tggattttcg aagagtataa gcattttact
     4441 gaaattttat caggtagttt aggatggcaa atgacattcc ctgtcaaaaa tggtaaaata
     4501 agaaaaaata atctgagaat cgaggaaaaa agatgcaaga aaagattttg gtaactggtg
     4561 gtgccggttt tattggaacc cacactgtta ttgaattgat ccaagcaggc catcaagttg
     4621 ttgtggtgga taatcttgtc aatagcaatc gtaagagttt agaagttgtt gaaagaatca
     4681 caggagttga gattcctttc tatgaggcag atattcgtga cacagctacc ctcagagata
     4741 ttttcaagca ggaagaaccg actggtgtca ttcactttgc tggtttgaag gctgttggcg
     4801 aatcaacacg tatccctctt gcctactatg acaacaatat cgctggaact gtcagccttt
     4861 tgaaagccat ggaagaaaac aactgtaaaa acatcatctt cagttcttct gcgacagttt
     4921 acggggatcc gcacacagtg cccatcttgg aagatttccc actttcagtg accaacccat
     4981 acggtcgtac taagctcatg ctagaggaaa ttttgactga tatttacaaa gcagactcag
     5041 aatggaatgt tgtcttgctt cgttacttta acccaatcgg agcccatgag agtggtgatt
     5101 tgggagaaaa tccaaacggt attccaaaca atctcttgcc atatgtgact caagtagccg
     5161 ttggaaaatt agagcaagtg caagtgtttg gagacgatta cgatacggaa gatggaacag
     5221 gtgttcgtga ctatatccac gttgtcgatt tggctaaggg tcacgttgca gctttgaaaa
     5281 aaatccaaaa aggttcagga ctaaacgttt ataaccttgg aactggtaaa ggttactcag
     5341 ttcttgaaat tatccaaaac atggaaaaag cggtgggacg tcctattcct taccgtatcg
     5401 tagaacgtcg cccaggtgat atcgctgcct gctactcaga cccagcaaaa gctaaagcag
     5461 aactcggttg ggaagcagaa ctcgacatca cccaaatgtg tgaagacgca tggcgttggc
     5521 aaagcaagca tccaaatgga tttgaagact aagatgatga tttcaatcat cgtcccttgt
     5581 ttaaacgaag aggaagtact tcctcttttt tatcaggctc tggaagcttt acttccagat
     5641 ttggaaacag aaatcgagta tgtctttgtc gatgatggat caagtgatgg gaccttggaa
     5701 ctcttaaagg cctatcggga gcaaaatccg gcagtccatt atatttcttt ctctcgaaat
     5761 tttggcaaag aagcagccct ttatgcaggc ttgcaatatg cgacaggaga tttggtggtg
     5821 gtgatggatg cagacctcca agatcctcct agtatgttgt ttgagatgaa aaatgtacta
     5881 gacaaaaatg tagacttgga ctgcgttggg acacggagaa ctagtcggga gggagaaccc
     5941 ttctttcgca gtttctgtgc tgttctcttt tatcgcctca tgcaaaaaat cagcccagtg
     6001 gctctgccgt cgggtgtccg tgattttcgt atgatgagaa ggtctgtagt cgatgccatt
     6061 ttaagcttga ccgagtccaa tcgtttttca aaggggctct tcgcctgggt gggtttcaaa
     6121 acctactatt tggactatcc aaatgtcgaa aggcaggctg gcaagacaag ttggagtttt
     6181 aggcaactct ttttatactc cattgaaggg attattaatt tttcagattt tcccttgatt
     6241 atagcctttg tagctggtct cctatcttgt tttctttctc tgctgatgac cttttttgtt
     6301 gtggttcgga ccctcatttt gggcaatccg acatctggtt ggacctctct gatggctgtt
     6361 attctctttc ttggaggcat tcaactcttg accattggga ttctcggcaa gtatatcagt
     6421 aagatttatt tagagactaa aaaaagacca ctttatctta tcaaagaaaa aagtgacctt
     6481 cctgatttta cagaaaaaaa taaagtgaaa agactataat tttacatgga aatgtgctaa
     6541 actagaggga gtggattatc gccattgatt taggcttcct ggtttccaat cagggcgcga
     6601 gttgggcaat ttttaacagc ctctaagaca tcctgactag gagaaatttc tttttccagt
     6661 tggtcagggt catcgtaaaa acgcacgatt ccattatcgt ggtaatcaaa taaatcagaa
     6721 taagtttggc aaagcccaca ggcgatacat cgttcaggta taagtgtgat tttcatgttc
     6781 atatttatat tgtaataaga aaagtaaaaa aaaacaagga gtaaggtatg gcaaaagaac
     6841 cgtggcaaga agatatctat gatcaagaag aatcaagagc agagcgtcgg catcgaaacc
     6901 acggaggggc tgataggatg gctaatcgta ttttgacgat cctagctagt attttctttg
     6961 taattgtggt ggtgatggtc atcgttctca tctatctatc atcggggggg agtaatcgca
     7021 cagcagcctt aaaagacttt catgattctg atgcaagtgt agtacaaatc tcatcttcaa
     7081 gcagttctca gcctgagcag agttcagagc cagaatctac ttctagtagt tcagaagaag
     7141 ctgctaatcc tgaaggaacg attaaagttc tcgcaggaga aggggaagca gctattgccg
     7201 ctcgtgcagg aatctccatt gctcagttag aggccttgaa tcctgggcac atggctacag
     7261 gatcttggtt tgctaatcca ggtgatgtta taaaaataaa ataggagccg atatgaaaac
     7321 aattcaaatt gctattgatg gtcctgcttc cagcggtaag agtacggtcg caaagattat
     7381 tgctaaggat tttggattca cctaccttga tacaggagct atgtatcgtg cagcgaccta
     7441 tatggctctt aagaaccaat tagtagttga agaagtcgaa gcccttctag ccttgttgga
     7501 ccagcatcca atcagctttg gacgttcaga aactggagac cagcttgttt ttgtaggaga
     7561 tgtggatatt acccatccta tccgtgaaaa tgaagtgacc aatcatgttt ctgctattgc
     7621 agcaattcct gaagtgcgtg agaaactggt ttctctccaa caagaaattg cccagcaagg
     7681 cgggattgtc atggatggtc gcgatattgg aactgttgta ttgccacaag cagaattgaa
     7741 aattttccta gtagcttctg ttgatgagag agcagagcgt cgttacaagg aaaatattgc
     7801 caagggaatt gaaacagacc ttgaaaccct aaaaaaggaa attgctgcgc gtgactacaa
     7861 ggatagtcat cgtgagactt ctcctctcaa acaagcagag gatgctgtct accttgatac
     7921 aactggtttg aacattcaag aagtagttga aaaaatcaaa gcagaagctg aaaaaagaat
     7981 gtaaatgtag aaaagccgat agattgatat cggttttttt ctaaatttgt caaaatgctg
     8041 attttagggt ataatagttg ctgttcgaga aattttgatt ttcaaagaat agtgaatgat
     8101 gataaggaga aaccatgaac aatttaccaa attgtccaaa atgtaactca gagtatgtct
     8161 acgaagacgg tgccctactg gtttgcccag agtgtgctca tgagtggaat cctgctgaag
     8221 ttgcagaagt agaagaaggt cttgtcgcta tcgatgccaa cggaaataaa ttgactgatg
     8281 gtgatacagt aactctcatc aaggacttga aagtaaaagg tgcgccaaaa gatttgaaac
     8341 aagggacgcg cgtgaaaaat atccgcatcg tagaaggcga ccacaatatc gactgtaaga
     8401 ttgatggttt tggtgccatg gaacttaaat cagagtttgt gaggaagatt taagcatgtc
     8461 aaaacactat aaacttgtat tttatagccg tatcttcttg tttctagcgg cttttacggg
     8521 agtttatctt gaaattacta agcatggtgg ttttgggatg cttctctatt acacggttct
     8581 gtccaacctc ttggtaacta tttttaccct ttatcttcta aaggttatga gccgtgtagg
     8641 tgaaaattgg caaaggccaa gtctcttgcg cttaaaaggt ggggtcacca tgagtatcat
     8701 gattacctgt gtgatttacc atttcctctt ggcgcccatt gcgactaatt tctataccct
     8761 agaaaatttc ctttgccact atatcgttcc catctggttt ttagcggata ccctcttttt
     8821 tgacaaacag ggtcaataca agatttggga tccagcagtg tggaccattt taccctttct
     8881 gtatatgatg tttgctcttt ttaatggctt ggttctaaaa ctcaatattc caaatgctaa
     8941 ggataatcct ttcccttact tctttttgaa tgtgaacaag ggttggaatg ttgtgtttaa
     9001 gtggtgtctg attatctttg ttgcctatat ggtagcagga tttattttct actttatcaa
     9061 gcaaatcaag agaaagtcat cctaatactc ttcgaaaatc tcttcaaacc acgtcagttt
     9121 ccatctgcaa cctcaaaaca ctgttttgaa caacctgcgg ctagcttcct agtttgctct
     9181 ttgattttca ttgagtataa gatacaaggg attcattttc gaatttaaga aggagtctac
     9241 aaaccaatga ggcttcttct tttcttgctt atttgataac tatcaaaaaa gtagaagatt
     9301 aagagaaata tagaaagaga tttcatgaaa caatttttag aacgggccag cattttggct
     9361 ctctccctcg ttttgattac ctccttttcc atttcgagtg ccctaccagc catgtttgac
     9421 tattatcagg gttattctaa ggaacaaatt gagctcttgg tgagcttgcc ttcctttgga
     9481 atcatgatga tgttactgct aaatggtttc ttagaaaaaa tatttcctga gcgcttacag
     9541 attagtttgg gcttgctgat tttatcattg agcggtacag ctcccttctg gtaccaagcc
     9601 tatccctttg tctttggaac acggcttctc tttggtttgg gtcttgggat gatcaatgcc
     9661 aaggccattt ctattatcag tgaacgctac caaggaaaaa ggcgaattca gatgttaggg
     9721 ctacgcgctt ctgcagaggt cgttggagct tctctcatta ccttggccgt cggtcagttg
     9781 ttggcctttg gttggacagc tatctttcta gcctatagtg ctggattttt ggtgctgccc
     9841 ctttatctgc tctttgtccc ttatggaaaa tcaaagaaag aagtcaagaa aagagcgaag
     9901 gaagcaagtc gtttaactcg agaaatgaaa ggcttgattt ttaccttagc tatcgaagcg
     9961 gcagttgtag tttgtaccaa tacagctatt accatccgta ttccaagttt gatggtggaa
    10021 agaggattgg gggatgccca gttatctagt tttgttctta gtatcatgca gttgatcggg
    10081 attgtggctg gggtgagttt ttctttcttg atttctatct ttaaagagaa actgctcctc
    10141 tggtctggta ttacctttgg cttggggcaa atcgtgattg ccttgtcttc atccttgtgg
    10201 gtggtagtag caggaagtgt tctggctgga tttgcctata gtgtagtctt gacgacggtc
    10261 tttcaacttg tctctgaacg aattccagct aaactcctca atcaagcaac ttcatttgct
    10321 gtattaggct gtagtttcgg agcctttacg accccattcg ttctaggtgc aattggctta
    10381 ctaactcaca atgggatgtt ggtctttagt atcttaggag gttggttgat tgtaatctct
    10441 atctttgtca tgtacctact tcaaaagaga gcttaggatt gattcctaag tttttctttt
    10501 gatactcaat gaaaatcaaa gagcatagtt gattgagttt ggaatagtac gctgtagctg
    10561 ctaaaatatt tctagaaatt aatttgactt tcctaataga gttgttcata tcttatttca
    10621 atctactata gaaagctagc cgcaggttgc tcaaaatagt gttttgaggt tgcagatgga
    10681 aactgacgtg gtttgaagag attttcaaga gtataagatt tttataccca tacaatattt
    10741 gttttaggtc cttgtttaca gactaatttc ttgataaaat agaggttatg acgagatata
    10801 aagcaactat ttcctatgat ggttatgcct ttgctggctt tcagcgccag cctcatgcgc
    10861 gtagcgttca ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg caaaccatta
    10921 ctgttcacgg tgctggtagg acagatagtg gggttcatgc cctgggacag gtcattcatt
    10981 ttgacctgcc ttatcagatg gatgaggaga aactccgttt tgccttggat acccagtctc
    11041 ctgaagatat tgatgtgatt tcgattgagc ttgtggcagc tgattttcat tgccgttatg
    11101 ccaagcatag caagacctat gagtttattg tggatagagg acgtcccaaa aatccgatgc
    11161 gccgtcacta tgccacccac tttccctacc cactcgatgt ggaacgaatg cagattgcaa
    11221 tcaaaaagct agagggaacc catgatttta ccggttttac agcctctggg actagtgtag
    11281 aggataaggt tcgcaccatc acagaagcta gtttaatagt cgatgagaca ggacaatttt
    11341 tgacctttac cttttccgga aatggtttct tgtataaaca gattcgcaat atggtgggga
    11401 ctcttctcaa aatcggtaac aaccgcatgc cagtagagca gattgatctc atcttggaga
    11461 agaaggacag gcaacttgca ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga
    11521 ttcgttatga agaataatcg tattttagca ctttctggaa atgatatttt tagtggtggt
    11581 ggactgtcag ctgatttggc tacctatacc ttgaacggct tgcatgggtt tgtagcagtg
    11641 acttgtttga cagccttgac agaaaaagga tttgaagtct ttccaactga tgataccatt
    11701 tttcaacatg aattagatag cttgcgtgat gtggaatttg ggggaattaa gattggtctt
    11761 ctccctactg tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg cccaggagta
    11821 cctgtggtgt tggatcctgt cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag
    11881 ctctgccaag agttgattcg ctttttccct tatgtcagtg tgattacgcc taatctccca
    11941 gaagcagaat tattatccgg tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg
    12001 cagaaattgc atgatttagg agcgccagca gtcattatca agggaggcaa tcgtcttagt
    12061 caggacaagg ctgtggatgt cttttatgat ggacagacct ttactatcct agaaaatcca
    12121 gttatccaag gccaaaatgt tggtgcaggt tgtacctttg cctctagcat tgccagtcac
    12181 ttgattaaag gtgataaact tttgccagca gtagaaagct ctaaggcttt cgtttatcgt
    12241 gctattgcac aagcagatca gtatggagta agacaatatg aagcaaacaa aaacaactaa
    12301 aatcgccctt gtatccctat taaccgccct ttctgtggtt ctaggttatt tcttaaaaat
    12361 cccaacacct acaggaattc taactctttt agatgctggt gtcttctttg cggcctttta
    12421 ctttggtagt cgtgaaggag cggtagtcgg aggactagca agtttcttga ttgacctctt
    12481 atcaggctac cctcagtgga tgttctttag cttggtcaac catggcttgc agggattttt
    12541 cgcaggattt aaaggaaaaa gtcagtggtt aggccttatt ttagcaacta ttgccatggt
    12601 aggaggctac gccttgggtt ctactttgat gaatgactgg gcagcagccc tcccagaaat
    12661 tctaccaaat tttatgcaaa atatggtagg gatgattgta ggatttattc ttagtcaaag
    12721 tatcaagaag attaagtaat actcttcgaa aatctcttca aaccacgtca gcttccatct
    12781 gcaacctcaa aacactgttt tgagcagcct gaggctagct ttctagtttg cactttgatt
    12841 ttcattgagt ataaagaggt tgagtaaaaa gtatttcaaa taagaaaaac gcatagtata
    12901 ggatgttgaa atgatgaact gccccccaaa agttagacag aaaaaatcta acttttgggg
    12961 tgttttatta tgaaactaac ttatgatgat aaagttcaga tctatgaact tagaaaacaa
    13021 ggatatagct tagagaagct ttcaaataaa tttgggataa acaatt
//
LOCUS       DAVHIT010000054        12988 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVHIT010000054 DAVHIT010000000
VERSION     DAVHIT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.055"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(39..1379)
                     /locus_tag="VVN16_001401"
     CDS             complement(39..1379)
                     /locus_tag="VVN16_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5572777.1"
                     /translation="MYIFWIILYTLITNGLEIVIFFKVDGIGLTFERIFKAFLLKILL
                     AFVFVMIGYMIGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQEQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKD
                     KLHDRLDQEQVLRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYDLVLKDSSKKLQDNKYDLGRLVNVRDRALKSLLAGKFIKAREKDIVFNVEVPEEI
                     QVEGMRLLDFLTIVSILCDNAIEASAEAGQPHVSIAFLKSGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIPTE"
     gene            complement(1392..2129)
                     /locus_tag="VVN16_001402"
     CDS             complement(1392..2129)
                     /locus_tag="VVN16_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572778.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(2134..2472)
                     /locus_tag="VVN16_001403"
     CDS             complement(2134..2472)
                     /locus_tag="VVN16_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572779.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVRYARRRYRE
                     IRQKWLKEGE"
     gene            2748..3077
                     /locus_tag="VVN16_001404"
     CDS             2748..3077
                     /locus_tag="VVN16_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU5572780.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3245..4294)
                     /locus_tag="VVN16_001405"
     CDS             complement(3245..4294)
                     /locus_tag="VVN16_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572781.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLVMGYGLPVFLVYVLLLGVGKYFLFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKVVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQSWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLISFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4291..5022)
                     /locus_tag="VVN16_001406"
     CDS             complement(4291..5022)
                     /locus_tag="VVN16_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572782.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5090..5500
                     /locus_tag="VVN16_001407"
     CDS             5090..5500
                     /locus_tag="VVN16_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU5572783.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5510..5797
                     /locus_tag="VVN16_001408"
     CDS             5510..5797
                     /locus_tag="VVN16_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572784.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            5959..6153
                     /locus_tag="VVN16_001409"
     CDS             5959..6153
                     /locus_tag="VVN16_001409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572785.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(6475..7611)
                     /gene="dnaJ"
                     /locus_tag="VVN16_001410"
     CDS             complement(6475..7611)
                     /gene="dnaJ"
                     /locus_tag="VVN16_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5572786.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEVKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(7816..8169)
                     /locus_tag="VVN16_001411"
     CDS             complement(7816..8169)
                     /locus_tag="VVN16_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572787.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDYTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(8182..10005)
                     /gene="dnaK"
                     /locus_tag="VVN16_001412"
     CDS             complement(8182..10005)
                     /gene="dnaK"
                     /locus_tag="VVN16_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU5572788.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(10485..11009)
                     /gene="grpE"
                     /locus_tag="VVN16_001413"
     CDS             complement(10485..11009)
                     /gene="grpE"
                     /locus_tag="VVN16_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5572789.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(11036..12070)
                     /gene="hrcA"
                     /locus_tag="VVN16_001414"
     CDS             complement(11036..12070)
                     /gene="hrcA"
                     /locus_tag="VVN16_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5572790.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREEQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(12234..12743)
                     /locus_tag="VVN16_001415"
     CDS             complement(12234..12743)
                     /locus_tag="VVN16_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU5572791.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         3923 a         2897 c         2411 g         3757 t
ORIGIN      
        1 agaccgagta acaagaactc tcggtcttat ttttttcatc attctgtagg tatcatagta
       61 agcacctgac gaaagacttg atcttgacag gtagtattta aactggcatt gggataactt
      121 tccacaatct tcataacggt atagagaccg acacctctct cctccccttt agaactaact
      181 ccaaaggaga agatttcaga aatatctatg ccctcttctt tgatggaatt ttcgatgatg
      241 aaggtctcct gtgctccact ttttaaaaag gcaattgaaa catgaggttg accggcctct
      301 gcactcgctt caatagcatt atcacaaagg atagacacaa tggttagaaa atcaagcagt
      361 ctcatcccct caacctgaat ctcttcagga acttcgacat taaagacaat gtccttttct
      421 ctagctttta taaattttcc agctaggaga cttttgaggg cacgatcccg aacattcacc
      481 aatcggccaa ggtcatattt attgtcctgc aattttttac tagaatcctt taagaccaag
      541 tcatagacct cttttatctg ctccatatcc tcctcttcaa tacccagacg taaacttgtc
      601 aagaggttgg tgtagtcatg gcgaaaactc cttacttcct tgtaaagttc ctctatatgc
      661 cgactatagc gttccatatc tctatagcgc aagacctgct cttggtccag tctatcatgg
      721 agtttgtcct tcaaataggt atccaatttc ttgataaccc ccataaaaaa taggaggtaa
      781 aacactagga tgagatggcg aacagtcgtt gattgaatac cttgctcata ttcaaagtaa
      841 gacagacttt gtatcaccaa atagtaagcc cccattatcc agttaatctt agtcagggac
      901 ttttgaaaac ctgtatctag aatctccctt ctcaaactag taaaatcata gtccaaccat
      961 ttcaaaaagg ctagagaaat gaagaaattg aaaattatta tacataaccc agtaaatgag
     1021 tagccatcat atacttgctc ttgtcccaaa aatggaagca caaaatagga gactcctcta
     1081 taaaagagat tcaccaatat cattggaaag agaccataaa agaaaaggag ttttttagga
     1141 agccctctta acaataagaa agacaagcct atgccataca agggttccat aaaataagat
     1201 aggtaaacat ttcctatcat atagccaatc attacaaaaa caaaggccaa cagtatcttc
     1261 aaaagaaagg ccttaaaaat cctctcaaaa gtgagaccaa ttccatctac cttaaagaaa
     1321 atgacaattt ctagcccatt agtaataagt gtatacaata taatccaaaa aatatacata
     1381 aatttcctag ttcagtgtaa gttattaata gcctcagaca cttccctgac cttataacgc
     1441 gcgatcagac agcttccacc attgggaaag aaaaggagtt tttctttctt atccaaatgc
     1501 accacatttg caggattgat gagaaaagag cgatggcact gcaagagacg gggttcctgc
     1561 ttaaaaacct cctctaaact cgccgtaaat tctagcctgt ccgtcttggt atagagaata
     1621 acacgatggg gtcttgggga tgtttcgaga tagtaaacct ctttgaaagg atattggaat
     1681 tgggcaaatt ttgatttaaa gtaaaagcaa tcttccgcca gacttttact atcttgacta
     1741 ttggcataga ggagggctgt ctcgatacga gattcaaact cctctgccga aagggcctta
     1801 tcaatgtagt ccaaagctga cacttggtag cgaaaggaca gaggcataaa ctccgagtga
     1861 gtcgtcacaa agacgattag ggcataaggg tcttgttccc gaatctttct agccacttcc
     1921 agtcccttca tctcttcatt tcgaatctca atatccaaaa agaatagctg atgggccccc
     1981 ttctcatgca cctctgccag cagttggtcc ggcttgccaa atacctcaaa agagctagga
     2041 atgatatgat gtgctttcaa aagtttctca atcgtcgttt caattctagt ctgttgggaa
     2101 aaatcatctt ctaaaacaaa tattctcatc ttcttactct ccctctttca accatttttg
     2161 acgtatttct ctatagcgac gtctggcata cctgacagca tattgacctt cttctccgag
     2221 aaaaaggatt ctttcttgaa aatcaatcga ttttaattta tcaagattaa ccaaacaatt
     2281 tcgatgacat ctcatcaaag tttccccaca ttggttctca agattactta aattttgaag
     2341 catattaaaa ctagcttttt ctgtaacaat ctgtacggta tgggctttag ttggatgtgt
     2401 ttggatatag tagatatcgt cgatgtttac tttatagact gttttctgtg tctgaataat
     2461 catatgtttc attggattct cctcctaaga aaagaataac agaaaagatc tagcccaagc
     2521 acacccaatc ctaaacagtc tttgaaaact cctaaaagga ttattttcta caacatcatg
     2581 atgagcttac aactatttta tcataaaatt cactcagcgc aattcaagac atttcaatga
     2641 caattaaaga tttgaataaa aaaaaagaat taaaagtgat aaactaacag tatcaaaaca
     2701 cagttgccag aagtaagact ggcacccaga ttaaaggagg cattcccatg acagatacag
     2761 accccatcaa aagagctcat actttgatta ctgacttaaa caaagcctac caagcatgca
     2821 aacaggcaag cgctgacgac gtccgctttc aggagcaatt aaactctatt cttggttttc
     2881 tagccaagac tgaaacagtg gataatcgat tcttgattga actagaaaaa ttttaccaga
     2941 cttccagtct tctcatggga ctcagcgctc ttgatccaga tgctccaact cgcgccgctt
     3001 ggcgggccta tgaccgcttc cactttgacc aagtcaagac caagttaata ctcaatgaga
     3061 atcaaagagc aaactaggaa gctagccgca agctgtactt gagtacggta aggcgacgct
     3121 gacgtggttt gaagagattt tcgaagagta taagtctcta cggaccaacc attatcctgt
     3181 agtaacaaaa aagaagctga gacaaactga actcaacttc ttttttagtg tcgtatcagc
     3241 aatgttagtc ctgcatctga cgttttacct gataaggcaa atacaagact agtaaaacca
     3301 aaccagctcc tagtaaggct agaagggcta gtttttcttg gaaggtaatc aacccaacaa
     3361 ctaattccac aagtaaaaca aaactgatca atcctcgaac tacctcctgt aagccttttt
     3421 ccttttgccc cttgtccagc ggaaagagtt gggtcaaata ctggtagtca aaggcatgat
     3481 agagggccag caactggaaa agcaagaggt agttaaagag aactaccact gctgtcgcaa
     3541 tccaagattg ctcgataaag acctgcgcca gcaaagaaag caagagcaga cggagactaa
     3601 gggcaaaaag gtctccattt cgcaaataag aacgcagata gagattttgc caaatcttcc
     3661 cagacacctt ctgaacaacc tttagaataa agtccagata ggcacgacgc ttgacgctgt
     3721 ttgaaattcc cttgacctgc gtaaagaggg caaagaaacg aagcaagact tgcttacgct
     3781 tgctttcttg ggaaatgaca tagtcccaat ccagtccagt ttcagtgaaa aatttgctgg
     3841 ccttttgacg aaagaggaaa tattttccta cccccaataa aagcacatag accagaaaaa
     3901 caggcaagcc ataacccatg actaaaaaca agggcgcaaa taacagcaag aagagggtct
     3961 gaacaaaaat ccaaaagact agggaaatgc cggtttgacg cttgagatgg agcttgattt
     4021 cctcttctcc aactaagaga aagagcttgt ctggagcctc catataggtg gcaattcctc
     4081 cccaaagtaa aagtaaaaca gacgtaattc ctacaaacaa aaggataggc caatgatttt
     4141 caggaaaatg ttgtaagagt tgactgtact ggtaggctag aaagcccaac aggacaagca
     4201 ggaacaagac aaagtggtca ttgagcacat agcgcagata acaaagacac tccttacgaa
     4261 aggcctgctt tctctttaaa aacaagtctt tcatagatcc tcctctttgg tcagagccaa
     4321 gtaaatatca ttcaaactag cctcaggcat atcaaaggct tcacgtagtt gcaggagatt
     4381 gcctttggca cgcacctctc ccttgtgaag aatgacaaag gcatcacaca tcttttccgc
     4441 cgaatccagc acgtgggtac tcatgagaat agacttgccc ttttgcttct ccacttccaa
     4501 aagctgaatc aaatcagaaa tagccagcgg atcaagacca aggaaaggct catccacgat
     4561 gaaaagactt ggatccacca caaaagcaca gataatcatg accttctgct tcatcccttt
     4621 tgaaaaatga acagggaacc agtctaattt ctgttccaaa cggaacattt ttaacaaggg
     4681 ctctactcgt tcgaaagcca ctttttgctc aataccgtaa gccatagcaa ccgtttcgat
     4741 atgctctctg agggtcaatt cctcatacag actaggcgtc tcaggaatgt agccaatctg
     4801 cttgcggtag ctagtcgcat ctccttgcag agtcaggcca ttgatattga tggagccact
     4861 ataaggtgcc aacagaccga taatctcatt gatcgtcgtt gatttcccag caccattgag
     4921 accaatcaaa ccgaccaact gcccactttc aacagtaaag gacacatctt tcaaaacagg
     4981 aacatgaaca tagccacctg tcaggttttt aatttctaac atattttctc caaatctggt
     5041 ataatgtagc tatattatat caaaattcag tacagtagag gtagatttta tgtcagattg
     5101 cattttttgt aaaatcatcg caggggaaat tcctgcttca aaagtatatg aagatgagca
     5161 ggtccttgcc tttcttgata tctctcaagt aacactagga cacaccttgg tcgtgccaaa
     5221 agaacactat cgcaatcttt tggagatgga tgctacgagc gccagccaac tctttgccca
     5281 agtaccaaaa gtagctcaaa aagtcatgaa agtcactaag gctgctggta tgaatatcat
     5341 ttccaactgt gaagaagtcg ctggtcaaac agtttttcat actcacgttc accttgtgcc
     5401 tcgctacagt gctgacgatg acctcaagat tgattttatc gcccacgaac cagactttga
     5461 caaacttgct caagtcgctg aaaccatcaa aaacgcttaa ggagttgcta tgaaattatc
     5521 caacctactg ctatttgcag gagctgcagc cggaagttat ctggttacaa aaaatcgcca
     5581 aaccatcaca gatgaagtct tgaataccac tgaccgcgtt caagctatca aggacgatgt
     5641 ggatattatc caaaacagcc tgcaaatcat taaccagcaa aaagaactta tcaaggaata
     5701 ccaagaagac ttgacttaca agtttaaggt cttggaaaag gatatccaga ctagactagc
     5761 tgtgataaaa gaaatgcagg gaactgaaga taagtaaaaa gagcccgacg gctctttttt
     5821 aaaacgtaaa caagttatag tgaattgaat ttagaatagc acatcatagt ttctaaagca
     5881 ttgttagaga ttactttaaa atcctcttat caatttgttc atattctatt tcaatctact
     5941 atatatatcc catcaactat gcatcataat ttaggtaact catactcaat aaaaatcaaa
     6001 aagcaaacta gaaagctagg cacagactgc tcaaaacacc gttttaaggt tgtggataga
     6061 actgacgaag tcagtaacca tacccatgac aagccgacgc tgacgtggtt tgaagaaatt
     6121 tttgaagagt atcattcacc atttcacaac taaatcgaag actttctgcc gtaatgaaaa
     6181 cacctgaaac agcttggttt cagctgtccg gaaactttaa gacttaggtt caaagtttag
     6241 gtatggaact tcgaaggagg tcgctaccgt ccgtcattac ttaagaaagt cttaaaaaat
     6301 ctataaagta aaaagagccc gacagctctt tttgtttata ctcaatgaaa atcaaaaagc
     6361 aaactaggaa actagccacg ggctgctcaa aacactgttt tgaggttgta gatacgactc
     6421 acatatatac ggcaaggcaa cgctgacgtg gtttgaagag attttcgaag agtattattc
     6481 tccatcaaag gcatctttta tatggtcaaa gaagcctttt ttctttggat ttactttcaa
     6541 gtcaccagca gtcgcgaatt ctttcaaggc tactttttgg cggtcgttca agcctgtcgg
     6601 tgttacgaca ttaacagtaa cgtattggtc accaactgca ccgccacgaa ggctcggtgc
     6661 ccccttacta cgtaggcgga atttcttacc agtctgagtt ccctctggaa taaccaattc
     6721 aacatcaccg tgaacagttg gaatatctac tgtatcacca agagccgctt ggacaaagtt
     6781 gaggttgaga ttgtagaaga tagtcgttcc ttcacgttca aacttgtcgc tagcttctac
     6841 agaaactact acatacaagt caccataagg tccaccgtta aagcctgctt caccttgacc
     6901 agcgaggcga atttgttgac ctgtttccac accagcaggg attttcacat gtacgctatg
     6961 agcttgtttc tcatgacctg ttccatgaca ggttgtacat ggatatttga cttcttttcc
     7021 tcgaccgtga cagacatcac aggttacttg gcgacgcatc ataccaagag gagtctgcgt
     7081 atcgacgtta atgacaccag cgccatgaca gcgtccacaa gtgactggac ttgtccctgg
     7141 cttagcacca gagccattac atgtacgaca gccagcttca cgatgatact taacttcctt
     7201 ctcagttccg aagatagctt cttcaaatgt caaattgaca cgatactgga gatcatctcc
     7261 ttggcgagga gcgtttggat tgcgcgaaga accgcctccg ccgaagaaac ttgagaaaat
     7321 atcctcaaaa ccaccgaagc cacctgcccc attgaaacca ccgaaaccac cagctccacc
     7381 aaaaccacca ttggcgcctg cagcaccata ctggtcatag gcagcacgtt tttggtcgtc
     7441 actcaaagtc tcataggctt cttgaacttc cttgtacttg tcctcagcac caggctcctt
     7501 gttgatatct gggtgatatt ttttggaaag cttacgataa gcctttttga tctcgtctgc
     7561 cgaagcgttt tttgacaccc ccagacgatc ataaaattca gtattgttca tacaagatac
     7621 caagaccgta aaattcgact gaaaaatagg aaatctgacg ctggagcgac gctcctagac
     7681 agatttctct tttttccgag aatttaggtc gggttcaatt cctttctttt atattgatga
     7741 gataatactc cagccactcg gccctatccc tttttaccga aaaacagagg ctgggttgca
     7801 acctctgtgg ttagattatt catttgaaat aaaaatggtt gtattatctc tagtagtaat
     7861 cttcaaagac cttttagcta aatcaaactc aaatttatta tagtgtgggt cttgtgtttt
     7921 atataggtgt aactcctgac atatatgatc cgaaaagtaa atcataacat ctttaaacac
     7981 agaagtgtag tcatatttac tcttaccaac agctacttct gtaaattctt tataaatgac
     8041 atttccattt ctttccacag tcaccagcat ttctgttgga ttataatcca aattaaataa
     8101 ttcttcaaaa aactttatct tttcatgata atctgcaatg tcagaaagat tatttttatt
     8161 aacactcata acactagccc cttatttttc agtaaactct ccgtctacga cgtcatcgcc
     8221 tgcgtttcct gttgcttgtg cgccttctgc tccttcttga gcttgttgcg ctgctgcggc
     8281 ttgttcgtag agtttcacag caaggccttg agctttttcg ttcaacgctt caagtttcgc
     8341 tttcatgtcg tccaagttgt tgtcttcttg agctttctta aggtcatcaa gggcagcttg
     8401 ggcagcgtca cgttctgcgt cgaagccttt accttcagtt tccttgattg tcttttcagt
     8461 cgcaaagatt gcttggtcta cttcattacg aaggtctact tcttctttac gtttcttatc
     8521 agcttcagcg tttgcttcgg catctttcat catgcggtcg atttcttcgt cagtcaaacc
     8581 tgagttcgat tggatgacaa tagtttgttc tttttgagtt ccaaggtctt tggccttaac
     8641 agacacgata ccgttcttgt cgatgtcaaa tgttacttcg atttgaggaa ttccacgagg
     8701 tgcagctggg atatcagtca attggaagcg tccaagagtc ttgttatctg ctgccattgg
     8761 gcgttcacct tgaagaacgt ggatatcaac ggctggttgg ttgtctgctg ctgttgagaa
     8821 gacttgtgat ttagatgttg ggattgtagt gttgcgatcg ataagttttg taaatactcc
     8881 acccattgtt tcgataccaa gtgacaatgg cgttacatca agaaggacaa cgtccttgac
     8941 atcaccagta atcacaccac cttggatagc cgcacccata gcaactactt catcagggtt
     9001 tactgatttg tttggttctt taccagtttc agctttaaca gcttcaacaa cggcagggat
     9061 acgagttgaa ccaccaacaa ggataacttc gtcgatttct gacaagctca aacctgcatc
     9121 tgaaagggct tgacgaactg gaacttttgt acgttcaaca aggtcacgag tcaaatcgtc
     9181 aaatttcgca cgagtcaaag tcatttccaa gtgaagaggt ccagcctcac ctgcagtgat
     9241 aaatggcaag ctgatttgtg ttgaagttac accagaaagg tctttcttcg ctttttcagc
     9301 cgcatctttc aaacgttgca ttgccatctt gtcagtagac aagtcgatac cgttttcttt
     9361 cttgaattct gctaccaagt ggtcaatgat tttttggtca aagtcgtcac caccaagttt
     9421 gttgtcccct gcagttgaca atacgtcgaa gacaccgtca cccaattcaa ggatagagac
     9481 gtcgaatgta ccaccaccaa ggtcaaatac caagattttt tcttctttgt cagtcttgtc
     9541 caaaccataa gcaagagctg ctgcagttgg ttcgttaaca atacgttcta cttcaagacc
     9601 agcaatttta ccagcgtctt ttgttgcttg acgttgagcg tcgttgaagt aagccggaac
     9661 tgtgataaca gctttggtta ctttctcacc aaggtagtct tcagcgtagc ctttcaagta
     9721 ttgaaggatc atagctgaga tttcttgtgg agtgtattct tttccatttg cagaaacttt
     9781 ttcagaagtt cccatcttag atttgataga gataactgta tctgggtttg tgactgcttg
     9841 acgttttgca gcatcaccaa cgataatttc tccgtttttg aatgagacta cagatggagt
     9901 tgtgcggttt ccttctgggt ttgcgatgat tttgctttca gttccttcaa gaactgcaac
     9961 tgctgagttt gttgtaccta agtcaatacc gataatttta gacatgtgtt tttctccttg
    10021 ttagttaatt ttctattttt ctttttgata ctcttcttaa gtggcgacgc cgtcagagct
    10081 tgactttgtc aatatctttg actaaacttt gagcctaagg tctcaaaatt tgcgcaaata
    10141 gcgccacagc gaagagtatc gtctttggtt cgcttcgctc actattacaa agccaaacat
    10201 attttatctt tcttcatagt ttattgtcgt ttcggacaag ttttctattc tgtaaattgc
    10261 gacacgagga gtcgaaatcg attttatttc gacgacgagt tagtaaggaa gctagacaaa
    10321 cgccatagcg attgccgttt tctgacgagt tgctttagct accgtcagaa ttgccgaatc
    10381 gaacacgccc taagctctga gtaaaaaaga tagattttct tgtgttcatc gaacacttcg
    10441 tcaatctcct atttttactg cgagcttttt acgggctttg tatcttagtt atacaccact
    10501 accattgctg ggcgtaggat gcggtcatgg agtttgtagc ctttttgaaa gacttgggcg
    10561 atggtatcta ctgggtgttc atcgtctgct gggagagttt ggatggccat atggtagtta
    10621 tggtcaaatt cgccatctgc tgcgatttct tcaattcctt cttctttcaa agcgtgaatc
    10681 aagctttctt gcaccatccc caagcccttc ttcacatcat ctgtcaaacc ttcaactgca
    10741 agtgcacgct caaggttgtc aagagatggt aagattgctt ttgccaagtc ctggctacga
    10801 taacgttgca agttttgacg ttcttcattg gcacggcgtt ggatattttg catttctgca
    10861 tgagcgcgaa gatatttgtt ttcgaactca tctgcacgtt catttgccaa gtccaactca
    10921 gacttttcag gagttgtttc ttcagctgtt tccacaactt cctcttcttg aacttcttct
    10981 acttcttcat tttttatatc ttgggccatg ttcgcctcct ttaatgattt caatcttaat
    11041 gtacttcata atgattactg ctgaggtagc ggtaaaaatc tgtcaacttc atagtcaaaa
    11101 cacggttgac cacattgact tgattgatta gctgttggta atccagatta actggaccga
    11161 taatggctag aattccaact ccccgataag gaatgaggaa cttactacta atcaccgcta
    11221 ggtcagctaa acaggactct tgaccgtctg caacacgaac attttgcatc tgttcctcac
    11281 gcaacccctc acgaatctcc aaggccacct tttgcggttg gtcaaagaac tgataggctg
    11341 ctagattggc aaaattcaag agatggacct tgcccgccat cacaatgttt tcgttgaaca
    11401 tttccttaaa gatgtgttca aagagatcga tgacattatc cgttgttgta aagtaacgct
    11461 ggataatctg cggaatctcc gtccgaatct tgtagtgaat atctaaaacg gtgtgaccga
    11521 ggaaacgttc ctgaatgatg ctcttcagtt tcagcaaatc ctcctgcaag aagttccttg
    11581 gaatcagaaa ctgactagta accgttcgcg actcgtctag ggtaaatacc gccaaggctg
    11641 tatgttgccc caaaacaacg atatcaaagg ctgtcaaacg ttgcctgctc ggctcaacat
    11701 ccagtgccac taccgtacag ccactcaggt ctgttagtaa gttagcagcc tcttgcagaa
    11761 tatcctccaa tttgaagaat tcctgatcaa aggctttgac aatctcatat acctcatttt
    11821 cagccagccg gtcaaaatcc agtgagtgtt tcacatagta ctgaaaacca gcaacacttg
    11881 gcatccgacc gcttgaagta tgagccttct caagcaaccc ttgtttttct agttccgcca
    11941 tgtcattacg aatggttgca ctgctagagt taatagactc ttgcaaggct tttgatccga
    12001 caggttcgtg cgttttggta aagatgtcaa taatcagatt taaaatatcc tgctgacgct
    12061 ctgtaaccat ctcatcactc ctctctgtct gatcaattag cactcaatgc atccaagtgc
    12121 taacttatga ttctattata cacccttaga agagaatgtc aagacaaaaa ctcaaaaaat
    12181 tagcactctt ttatcaagag tgctaaaaat caatctgaag acctagaacc ttgctattca
    12241 tctactcggt atttcctttt tagtctgaaa taaccatgaa ttagataaga aatcagaaaa
    12301 gcatataaaa caaaaataag aaagaaagac atagaaagat cctctctaaa caaactcgta
    12361 caaacaacaa gaccacctaa gaaagtagct gtacaggaac gaagtctaga tcgcataact
    12421 tcccatttga gccccttact catatagact tgatcctctg gaagtaaatc agaagacgat
    12481 ttacgaagaa tcgaacaaga acctgctcct atcaattccc aacctctatc tctaaaatct
    12541 tgcagttcat gcttatattt tttaagaaat ctagaatcat agatacggta gatgacatcg
    12601 tctggttgac attggtcaaa atagaacaaa ccaaaacgac tcgttctata cctccaacct
    12661 ttcaaatgca tctcatgtaa atattcttct tccttgtcca aatcaacaat ggtgaaaatc
    12721 cgaaattcta ctctgctatt cattgtctta ccccaaaatt agaaaacatg cctggcgtta
    12781 tttattagat aattctttcc acttttgact caatctccaa aaaatataag aaatctgaat
    12841 cgcaaaaact atcaataaaa cccaatctat tatgaaaatc aaaaacactt tccaactgaa
    12901 agaactacct ccagtgacaa actttgagaa aaacggcagt agagctaaaa agagaagtaa
    12961 aataggaagc atccgcattg ttaaaatc
//
LOCUS       DAVHIT010000055        12859 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVHIT010000055 DAVHIT010000000
VERSION     DAVHIT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.056"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(209..1177)
                     /locus_tag="VVN16_001416"
     CDS             complement(209..1177)
                     /locus_tag="VVN16_001416"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU5572792.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1322..2090)
                     /locus_tag="VVN16_001417"
                     /pseudo
     CDS             complement(1322..2090)
                     /locus_tag="VVN16_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(2115..2612)
                     /locus_tag="VVN16_001418"
     CDS             complement(2115..2612)
                     /locus_tag="VVN16_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5572793.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2685..4046)
                     /gene="radA"
                     /locus_tag="VVN16_001419"
     CDS             complement(2685..4046)
                     /gene="radA"
                     /locus_tag="VVN16_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5572794.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVATIQEVLKK
                     VFA"
     gene            complement(4060..4575)
                     /locus_tag="VVN16_001420"
     CDS             complement(4060..4575)
                     /locus_tag="VVN16_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5572795.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLKVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4577..5020)
                     /locus_tag="VVN16_001421"
     CDS             complement(4577..5020)
                     /locus_tag="VVN16_001421"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU5572796.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5303..5668)
                     /gene="lytA"
                     /locus_tag="VVN16_001422"
                     /pseudo
     CDS             complement(5303..5668)
                     /gene="lytA"
                     /locus_tag="VVN16_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
     gene            5688..5855
                     /locus_tag="VVN16_001423"
     CDS             5688..5855
                     /locus_tag="VVN16_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEU5572797.1"
                     /translation="MANTPIKPGTDNQKPGRYVDVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            complement(6010..6213)
                     /locus_tag="VVN16_001424"
     CDS             complement(6010..6213)
                     /locus_tag="VVN16_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572798.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(6236..6427)
                     /locus_tag="VVN16_001425"
     CDS             complement(6236..6427)
                     /locus_tag="VVN16_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5572799.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            6998..7366
                     /locus_tag="VVN16_001426"
     CDS             6998..7366
                     /locus_tag="VVN16_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5572800.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            7366..7599
                     /locus_tag="VVN16_001427"
     CDS             7366..7599
                     /locus_tag="VVN16_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572801.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            7599..7862
                     /locus_tag="VVN16_001428"
     CDS             7599..7862
                     /locus_tag="VVN16_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU5572802.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            7875..8255
                     /locus_tag="VVN16_001429"
     CDS             7875..8255
                     /locus_tag="VVN16_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU5572803.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            8272..9342
                     /locus_tag="VVN16_001430"
     CDS             8272..9342
                     /locus_tag="VVN16_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEU5572804.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            9397..10539
                     /locus_tag="VVN16_001431"
     CDS             9397..10539
                     /locus_tag="VVN16_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5572805.1"
                     /translation="MWMEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKIKQKLGEKQHSVSNITFEKLYEEFEENWKHGVKNSTVYASKNVKKEILKQIE
                     GDYLVRNIDRRLLQKVIDQLLQDGRSHNYVSKIKFKLNQIMKFAIRMNYIDTNEMLFV
                     ETPRKVITSDELRKKNTKYLDQKEFKLFIQNLKDEALCDYRITKYIRIAKVLFLTGMR
                     YGELAALNYKEDIDFSKKTIHIKHTYDFRQKERTTPKTIKSDRVITAPQKVLDIIKEQ
                     IIENATNGFDTDFIFINTLGEPITNARVICALKRHGQKIGIEKNITTHTFRHSHISLL
                     AELGIPLTAIMDRVGHSDSKTTLEIYSHVTQKMVSDISSKLDKIKF"
     gene            complement(10622..10715)
                     /gene="ffs"
                     /locus_tag="VVN16_001432"
     ncRNA           complement(10622..10715)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VVN16_001432"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10748..11215)
                     /locus_tag="VVN16_001433"
     CDS             complement(10748..11215)
                     /locus_tag="VVN16_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5572806.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(11416..12702)
                     /locus_tag="VVN16_001434"
     CDS             complement(11416..12702)
                     /locus_tag="VVN16_001434"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU5572807.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         4211 a         2924 c         2140 g         3584 t
ORIGIN      
        1 cctactagaa ttctccttag aaagcgtcga ttcaagtgct atcactgttc aaaaatgatg
       61 gtcgctgaaa cttctgatga cgtatagtca tatttcttct ctttttatta tatcacagtt
      121 ttaaatctag ctttactaga ttcaccgcta ctatccattt attcggaaaa aagacgaaaa
      181 aacctgagaa tcatctcagg cttggtcatt aaattttttt ctcaatatcg aaaagtggag
      241 aaagtggtcg tttttcatga atacgtacga tagcatcccc taggagatga gcgattgaaa
      301 tctgctcaat cttatcaatc aaacgctctt ctggcagata gatggtatcc aaaacaacca
      361 atttcttaat agctgatttt tggatattgt ccgtagcagg accagaaaga actgggtgcg
      421 tacagcttgc atagacttca acagcaccag cttccgcaag agcatctgcc gcatgacaaa
      481 tcgttccagc ggtatcaatc atatcatcaa tcaagataca agtcttgcct tcaaccttac
      541 cgatgatgtt cataacttca ctagtattca tcttatcaac gctacgacgt ttatcaataa
      601 tagcgataga tgttttcaaa aattctgcca acttacgagc acgagtcacc cctccatggt
      661 ccgggctgac aaccacatag tcagaaccaa ccataccacg ccgctcaaaa taatctgcaa
      721 ttagaggagc acccatcaaa tgatccacag gaatatcaaa gaatccttga atttgcgcag
      781 catgcaagtc gatggtcaat aaacgatcca ctccagctac ttcaagcata tttgcgacaa
      841 gttttgaagt gattggctca cgcgctctcg cctttctatc ctgacgtgca tacccatagt
      901 aaggcatgac aacattgaca gactctgcac tcgcacgctt caaagcatct accataatca
      961 aaatttcaag cagattgtca tttacaggcg aactagttga ttgtaagata aagacgtgtt
     1021 tcccacggat tgattcttca atgttgacct gaatctctcc atctgaaaat tggcgaacac
     1081 ttgatttccc caactctatc ccaatctcct gcgccacacg ttctgccaat tctttattag
     1141 aagaaagggc aaacagcttt aaatcagaaa aagacatgat ttcctccggt atatatgtat
     1201 cgcttgtgct tttcacaaga ttttccatct accattgtag cgctttttgc actatttttc
     1261 aatcaaaaat aaaagaaggg caccatattt gtacccttgc atcattcttt tgaaaaatat
     1321 tctaggtcat caactcattg tgtttctcaa caaagcaata agcatgataa taaccataga
     1381 gagcaatagc cgtaaccact ggaatcgcta aaggcaactc tgtttccaac tccacaaaag
     1441 gagagttaaa caataagacc ctgtttcttg ccaaccttct gtcctttata ggctctgtaa
     1501 agcaagtaaa cacctactac agctagacct gaaaaagtcc agtgagaggc aattcctgag
     1561 atgatacgct ctaaaattcg cgaaatagta aagtcaaagc cctctggcaa atccgtacga
     1621 atataaccaa tatcctcaat catttggaat cccaaaccgg aggcaattcc aagtaaaaac
     1681 aaaaatttta attttcgcac aggaatcaaa gccaaaacaa aaacaagtgg caataatttc
     1741 aagggttctt ctaccaaagg agccacaata gcactttcaa aggcatttaa aaatgtacta
     1801 tctgggaaaa gaacccccag taaatcatgg atataagtat tagcaaaact agacaaccag
     1861 cctgaaagga acatccctcc caataaagac agaatcaaaa ccttctttgg caattcccat
     1921 tttttcccaa tacggaagag aaaataaaga gccggaatca tgtaaaagag agctagaaag
     1981 atagaaactc ccattagtcc atattccgca cctgacctcg aaccgtccgt atagtagatg
     2041 gtttcatact gtaaaccaat acatagcaat aaaatactgc ttttcttcat acactttctt
     2101 tctaaatgaa gtatttataa ttctacgact gtcatacttc ctgtatcaac attgtaaata
     2161 gcaccagaga taatgacatc gtctggtatt aggggagact cgataagcag ttgcatatcc
     2221 tcgcgtacac tctcttctat atcttggaag ggcaagaagt cctggtctga cacatctaca
     2281 cctaattcct cttttaaata ctcctgaaaa ggttcatttt caaaggtctg agcaccacag
     2341 tctgtatggt gcaataccac aatctctctt gtccccattt gttgctggga aataactagc
     2401 gaacgaatca tgtcttcagt cactcgacca cctgcattcc gcaagatatg agcatcccca
     2461 agtgccaagc ccagagcttg cgcaacgtgc agacgagagt ccatacaggt cacaatagct
     2521 actcttgttt tgggtttaag tggcagattt aactgcccat gtagggcaac ataagcctga
     2581 ttagcttgca taaactgttc aaaatacgac acgattccct ccttgaaaat ttgatagtca
     2641 aatatttctc ctatcttatc atttttaaga gaatttgtca cggattatgc aaagaccttt
     2701 ttcaaaactt cctgaatcgt tgccacgcca atgacctgaa tttccttagg cagagtgatt
     2761 cctgtcaagg aattcttagg tacataaatc ttagtaaagc ccagtttagc agcttcgttg
     2821 atgcgttgct caatacgatt cacgcgccga atctctcctg tcaagcccag ttctccgaca
     2881 aaacattcct gaggattagt tggcttgtct ttgtagctcg aagcaatagc aactgcaaca
     2941 gccaagtcaa tcgcaggttc atccaattta acaccaccag cagatttgag ataggcatcc
     3001 tgattttgca agagaagccc tgcccgtttt tccaaaacag ccataatcaa gctagcacgg
     3061 ttaaaatcaa gtcctgtcgt agtacgcttg gcatttccaa acatggtcgg tgttaccaaa
     3121 gcctgaacct ccgccaaaat cggacgcgtc ccttccatgg ttacaacgat ggaggaacca
     3181 gtcgccccat ccaaacgctc ttctaggaaa acttgactcg gattgagtac ctcaaccaag
     3241 ccgcccgact gcatctcaaa aatcccaatc tcattagtgg aaccaaaacg atttttgacc
     3301 gctctcaaaa tacgaaaggt gtggtgacgc tccccttcaa agtaaagcac cgtatccacc
     3361 atatgctcca acatacgagg cccagccaag gttccttctt tggtcacatg acctacgata
     3421 aagatggcaa tgttattggt cttggccaac tgcatgagtt cagcggtcac ttcacgtacc
     3481 tgagaaacag acccctgcac ccctgaaatc tcaggagaca tgatggtctg gatggaatca
     3541 ataatgagaa agtctggctg gatacgctcc acttctgcac gaacactctg catattggtc
     3601 tctgcataga gataaaactc actatcaata tcacctaagc gctctgcacg tagtttaatc
     3661 tgctgggcag actcctcccc actgacatag agaactgtcc ccacttggga caactgggtt
     3721 gagacttgta ggagaagagt tgatttccca atcccaggat ccccaccgat gaggacgaga
     3781 cttcctggta ccactccgcc tccaagcaca cggttgaatt cctccatctc cgtcttggtt
     3841 cgattgacat tgatggaagt cacctcagct agtttcatgg gcttggtttt ctcacctgtc
     3901 aaggacacac gcgcattctt gacctcggca acctcaacct cttccacaaa agaagaccaa
     3961 gacccacagt tggggcaacg tcccagatat ttaggggaat tatacccaca attttgacat
     4021 acaaatgtcg cttttttctt tgcgatgaca aacctctttc tatatctcta actcacactc
     4081 aatcacttgg caaaaatcaa tcttctcatt tggcacaaac tggcgcatga gcattcgatg
     4141 agcaacaact accacagtct gatgttctcg atacttagac atacattcta gaaaccgaga
     4201 cttcatttcc gtagctgtct catattgaat aggactatta ggaagcaact cccccttgtt
     4261 ttctaaaaac agtcttctag ctgtttcaaa gttttctatt cctgttttat agacctgcca
     4321 ttcatgtaat aaaggctcta cttttaaagg aagacccgta gcacagacca catacgaagc
     4381 cgtttctaaa gctcttgtga ctgcagaaga tacgattatt tcagctgacg agagtaaagg
     4441 atttttgctc aatttctgga cttgctgccg tcccatctca gacaagggtg ccaaatctat
     4501 cccaaatcct atataagaac gctcctctaa ctcacggtaa tctggctccc catgacgtac
     4561 aaagataatc ttcattctag tgccctgtcg atccaaatcc accagttcga acgccatcag
     4621 ccgcatctcc atctgcaatt aagaaagtag caaaaacagc ctggacaata cgctccccaa
     4681 cttcaagaac aacctcttgg tctgtgatat tcttcatctg cgcaaaaata tgcccttcat
     4741 ttccaggatt tccataataa tctccatcaa tgaccccaac tgagttaatt aaaaccaagc
     4801 ccttcttacg aggatttgaa gaacgatcat agaggtagag aacctcagtc ggctgcatat
     4861 aagccttaac ccctgtcgga accaagacaa tctctcctgg cgcaacaact gtacgcacag
     4921 caacctttaa gtcgtaacca gccgcatgcg ctgtctcacg cttgggcaat aaattttcat
     4981 ctgtaaaact cgaaaccaat tcaaaaccac gaattttcat aattttctct tttctattat
     5041 catttattct agattattct atcttattta ttcggaaaaa gcacgaaaaa agagcacaca
     5101 acaattatag gcgatacgat aatttacact gtttcacaat acgttgaaat ttagagcttt
     5161 aaagcgaggg caaagttgat tttttacatc gttttacaga ggtttacgac acttttgccc
     5221 cttttttgcc ccttttttaa aacaaaaaaa ccgcaagcct gagcctgcgg tgaaagaaca
     5281 atttagaaag tttcctttct atttatttaa ctgtaatcaa gccatctggc tctactgtga
     5341 actctggctt atctgccagt gttccgtctg gtttgaggta gtaccagcct gttccgtctg
     5401 ctgactggat aaaggcattt gataccatgg cgccttcttt agcgtctaag tagtaccaag
     5461 tgtccttgta cttgacccag cctgtcttca tggcaccttc tacatcaaaa taataccact
     5521 tgtcagcgat tttcttccag cctgtggcca tttcgcctga ttggtcaaag taataccaat
     5581 taccgtctgt gtgcttcttc cagcggtctg caagcatata gcctgagcca tcgaaataat
     5641 accaggtacc gttgattttc tcaaacttat agaaaggagg agatagtatg gcaaatactc
     5701 caataaaacc tggaacagat aatcagaaac ctggtcgcta tgtagacgta ggacctcgtg
     5761 gcggaaaagt tactaatggt cataccgcaa ctatcggaaa aggtgatcgg ctccctccga
     5821 catctgctaa aggcaacggt tggaaaaaag tctaatcttc gtttgcgtac aatcgttcaa
     5881 tggttgtacg ctttttccat acacaaaagc acattcccaa aaaatcaatt tgaatccatg
     5941 cttcggcgta atctttccca ttgctggcat agtgagttat ataatggtga atcattttat
     6001 tccttcctcc tattgagtta cttgagaagt atcatgaatg gcttcatagc taagacgctt
     6061 aaattcttct gagtctatct gaaaattgat aggctttttt tgtaaacact caagaaaact
     6121 agtgtttctt aaaagttttt caactaactc agggtctgcc tttacaaagg tggactcttt
     6181 ttgtccacta tacggatatc gttttggtct cattttccta ctcctcaaat ctttcctact
     6241 caatcccata atcttcaata acctgaagaa tgaaactgtt cgctcgtgga cctttagtcg
     6301 ttccacttag aatgtttgtc acttcctgtc gtttaaagcc gtaagcaacc gctagagttg
     6361 cttttttaat gcctttctct ttcaagaaag caataactct ttcgcgaccg tttgcgatat
     6421 ctggcatatt ttctcctttc tttttctttc ttctttttct gctataatat aagcagaaag
     6481 gagttaacct tatgactttt aaagaatatt tactcaaagc aagcaaacgc gacatctacg
     6541 atgatggtaa agattttgag aaatcaattt cagattctca ctaatcgagc gtgacttttt
     6601 tgatatggca atctcagaaa tttaccacca gcgctgacta ccttaatgac tttttcaagg
     6661 tggtcttttt cttgttctaa atttttaaga atacttacca tcagcacgtt cctcctcttt
     6721 acttatttgt aaataagaaa caactaaaaa tgtaactatt tttctgcatt atacttgaca
     6781 acttacacca aatcggctaa aattaaagca taataaaaac accaataaat ctataaatac
     6841 cgttcgccaa aacattttta taatttattt cttagttgtt tttttagttg tttatcactt
     6901 acaaaaaaca ttttacacct tttgggataa ttagtcaacc tttttacacc aaatttgtta
     6961 aatttttttg tgatgtctta gaaaggttga tttaacaatg tttgagacat ttgaaaaaat
     7021 aaaagaattg gcaaaaaagc gtggaaaagc tcttggacaa gtagaagaag acttaggtta
     7081 tggtagaaat acactctata agataaaaaa ttctacgcca aacgctgaac gtatcgcaga
     7141 aattgctaat tatttcaacg tatccaccga ctacctgctc ggacgaacag ataaccctgc
     7201 tatcgctggg agtgatgaat ttgcccaagt aaacggacaa atcatagact tacgcaaagc
     7261 agcagccaac accatgttat ttgacgggaa accactaaat gaagatgata tcgacttcat
     7321 tacatccgtc ctatccgccc acttcaaaag caaaggagaa cgctaatgac tatcactatc
     7381 aacttcacag aaaaaaattc ctacattacg gactacctaa acaaacacgg tatcgacaca
     7441 acgaccatgg attttgacga cttcatggca ctcatggaag atatcgaaga cgcacgagca
     7501 gccgaccaag cctatatgga gtatttagcc gacccagcta cttataccat ggatgaggtc
     7561 ttggatgaac taggactaac tcgagaggat attgcttaat gtatcggcta gatattgata
     7621 aaaaagctct caagcaactt aaaaaactag ataccccaac cagaaaacaa atcctatcct
     7681 ggcttgctaa aaacattgaa aacacgacca atccacgaca acacggaaaa gcactaaaag
     7741 ccaaccttgc aggttactgg cgatacagag tagagaatta ccgcatcatc tgtgatatcc
     7801 aagacgataa actagtcgtc ctagccgtgg aaatcgccca ccgcagagat gtctataaat
     7861 aacgaaggag aactatgaca ctcgctaaac tctgcgaaga atatcaagta gaactttgtc
     7921 tcttcgacgg ttcaaactgg cacaatagcg gtttctacaa tccagacaca aacgtactcg
     7981 ctattgacca caacttgact cctgaacaac aaatccaagt cgccctacac gaacttggac
     8041 acaaagacca cacacgctca gagtaccaga acgcccgtct acgctgtgaa aacgaagctg
     8101 ataggaatat gatccatcat ctcgtaaaag acgcactaga aaacttagac gaccccacag
     8161 agtttgatta cctcaaattc atgtcttact acaatctaaa aaccatgaca aatgaaatca
     8221 tggttaaaga agagtattta gcattagtaa attaaaaaaa ggagtatcaa tatggaaatt
     8281 gataaagtaa aagccgattt aaaacaagtc ggcaaacgtg tagcagacct cagccaaagc
     8341 attacgaatg aagaacaaac aaagaatgcc ttcattatgc cgttcttcca agcgcttgga
     8401 tatgatatct tcaatccact tgaatttgtc ccggaattta ctgccgacgt gggcattaaa
     8461 aaaggcgaaa aagtcgacta tgcgatcatt ttggatggcg agcctcaaat cctaattgaa
     8521 tgtaaatcaa ttacagaaaa ccttactaag cacgactctc aactattcag atatttcgta
     8581 actactaaat caaaattcgg tatcttgaca aatggtagag aatataaatt ctttactgat
     8641 ttagatgagc caaataaaat ggacacaact ccattcttaa caatcgatgt gactgatatc
     8701 aaagaaaatc aatttactga aatcatcaaa ttccacaaag agaattttga tattgataat
     8761 attgtttcat cagcttctga attgaaatac ctcaacaatc ttaaagcgtt ccttactgaa
     8821 aatatcacta caccgtcaga tagcttcctc agatatctaa cttctgaaat ctatgaaggg
     8881 cgtgttactc aaaatatctt aacgacattt tctcccatta ttgtgaaagg gtttaatcaa
     8941 ttcatcacag agcgagttaa tgaaaaatta agtgctgcac ttaatacaag cgttgaaacc
     9001 aaagtaacaa ccgatattcc aaaagttgaa gctgaagctg aagagattgt cgaagtaact
     9061 gacgaaatta tcacaactcc tgctgaatta gaagtttata ctgttgttaa aatgcttgct
     9121 agggatgtag tttctccaga acgtgtattt tacagagata accgtagcta tttcaatgtt
     9181 ttggttgacg ataacatcaa aaaatgggta ttacgttatc gttcaaattc aaaaaaaagc
     9241 actatcgaaa tccgtgataa aggcatcttc ccggtgtcta cccctctcga agtcgcaaat
     9301 tatgccaatg aaattttaga agtaatcaag aaattctcat aataaaaaaa gccctcttta
     9361 ctatacccat tttatcaaaa aagtgaggta aaatcaatgt ggatggaaga attgccaaac
     9421 ggcaaatata aattttttga gagatacaaa gatccatata ctgagaaatt aaaaaaagtc
     9481 tcagtaacga tggagaaaaa aactccccaa gcaagaaatc aagctgcgat tttacttcag
     9541 gaaaagataa aacaaaagtt aggagaaaaa caacattctg tttctaatat aacttttgaa
     9601 aaactatatg aagaatttga ggagaattgg aaacacggcg taaaaaactc aaccgtctac
     9661 gcttcaaaga atgtaaaaaa agagatttta aagcagatag agggcgacta cctagttaga
     9721 aatattgata gacgtttatt acaaaaagta atagatcaac tattacaaga tgggagatct
     9781 cataactatg tttctaaaat caaattcaag ctcaatcaga taatgaaatt tgctatcaga
     9841 atgaattata ttgatacaaa tgaaatgcta tttgttgaaa cgcctagaaa agtaattaca
     9901 tccgacgaac tcagaaagaa aaatacaaaa tacttagacc aaaaagagtt taagttattc
     9961 atccaaaatt taaaagacga ggccctatgt gattatcgaa ttacaaagta tatccgaata
    10021 gctaaagttc tttttcttac tggcatgagg tatggagagc tggcagcctt aaactacaag
    10081 gaagatatag atttttctaa aaagactatt cacatcaagc atacatacga tttcagacaa
    10141 aaagagagaa ctacaccaaa gacaatcaag tccgataggg ttataacagc acctcaaaaa
    10201 gtgttagata ttatcaaaga gcaaataata gagaatgcga caaatggatt tgatacagat
    10261 tttattttca tcaatactct aggagaacca ataacaaatg ccagggttat ttgtgcattg
    10321 aaaagacatg gtcaaaaaat cggcatagaa aaaaacataa ctacacatac atttagacat
    10381 tctcacatat ccctacttgc tgagctgggc attcccttga ctgccatcat ggacagagta
    10441 gggcatagtg actcaaagac cacactagag atttattctc acgttactca aaaaatggta
    10501 tcagacatat ctagcaagtt agacaagata aaattttaaa ttgtgcccct cgtctgcccc
    10561 tttttcttat acaagacaaa caaaaacccc ttaaagtgtt gattttaaag ggtttttaaa
    10621 gtgcacgaaa aaagagcaca caattcaaat cgcttagggc tgctggattc ctcccctgac
    10681 ccgcttcacg cagaactgtt gctccactat ttattatatc acattcctat tcattttaaa
    10741 agcaaaatta ttttttccgt ctatttctaa aaaaatcctg catgatagct gcgcattcat
    10801 cttccaaaat tcctatttca acctccacac gatgattgag acgctcatct gtcaagatat
    10861 cgtacaaact cccagcagcg ccaaatttct ggtttttagc cccatagacc acatttggaa
    10921 tgcgggcaag cccaatcgcc ccactacaca tgacacaagg ttcaatggtc acaaaaagtg
    10981 tgcaatccag caagcgccag ctctcctcac tcaagttcgc atcctctata gccataattt
    11041 ccgcatgcat aaccgctcgc tgtaattcct cacgcgcatt atgcccacga ccaatgattt
    11101 ccccatcttt gacaatcaca caaccaattg gaatttcatc gtgttcaaga gcaatctcag
    11161 cctctctcaa agcctccctc ataaagactt ctttttcttc aagcgtataa tacatctctt
    11221 ttctcttttc ctacttatcg attttattat tatatcatga atcccaacac aaaaaaagcc
    11281 accgaatgcg gtgactctat agggagatta ttatgaaaaa gaaaagttta ggatatttgt
    11341 tacaacaagt taggaggtct tcttgtaacc gtctatagta tacccgacct atcttaaaca
    11401 aatcttaaaa atctcttagg accaaacact ttctaaaata tttgtttgtt cacgaccagg
    11461 acctactgag aaagtagaaa tacgaacgcc aaccaattca ctcacacgac gaacatagtt
    11521 acgcgcattc tcaggaagat cttccaaatt gcgaactccg gtaatatctt ctgaccaacc
    11581 tggcaactct tcatagatag gcttgcaacg tttcaattgc tcaagactag ctggatagta
    11641 gtcaatacgt tgaccgtcaa gatcataggc cacacagatt ttcacagtat ccaaaccgct
    11701 caaaacatca atagagttca aagaaaggtt agtaatacca gaaacacgac ggctatgacg
    11761 catcacaact gagtcaaacc aacctacacg acgtggacga ccagttgttg taccatattc
    11821 atgacccact tcacggatac gttctcccac ttcatcaaac aactcagttg ggaaaggacc
    11881 atctcctaca cgactcgtat aagctttaca tacacctaca accttgtcaa tcttgcttgg
    11941 accgatacca gaaccaattg tcacaccacc agctacaggg tttgatgacg taacaaatgg
    12001 ataagtacct tggtcgatat ctagcataac accttgtgca ccttcaaaaa gcacacgttt
    12061 gccattatca agcgcatcat tcaagataac agatgtatct atcacgtatt tcttgatttg
    12121 ttgaccatat tcgtaatatt cttcaaaaat atcatcgaaa acaatcgctt tactgtcata
    12181 caatttttca aaaagacgat tcttttcagc aaggttacgt tctaaacgct cacggaaaat
    12241 atctttatct aaaagatctg caatacgaat tccaacacga gcagccttgt ccatataagc
    12301 tggaccaatt cccttaattg tcgtaccaat cttattgtcg cccttagctt cttcttgcaa
    12361 gcgatccaac tcgatatgat aaggcaaaat aacatgcgca cgatcagaaa tacgcaagtt
    12421 atcagttgtt acaccttcct catgaagata gctcaactct tttacaagag atttaggatt
    12481 tacaaccata ccattcccaa tgacagatat tttttcaggg aagaaaatcc cagatggaat
    12541 caagtgcaac ttaaatttct taccgtcaat cacaatcgtg tgaccagcat tatcaccacc
    12601 ttggtaacgt gcaatcactt ctgcattcgc tgaaaggaag tctgtaatct tccctttacc
    12661 ttcatcaccc cattgggtac ctacaacaac aactgaagtc ataatcttgt ctgagccctc
    12721 aggctcttcc tttctcacat acatggcagg actctcacct gcaattatat cttacaattt
    12781 attataataa aaaatcgcct ttttatcaag aagaaacaat agaaagattt gctatttcca
    12841 actattaaaa aatgattta
//
LOCUS       DAVHIT010000056        12189 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVHIT010000056 DAVHIT010000000
VERSION     DAVHIT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.057"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(363..1556)
                     /locus_tag="VVN16_001435"
     CDS             complement(363..1556)
                     /locus_tag="VVN16_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5572808.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1694..3136)
                     /gene="gtfA"
                     /locus_tag="VVN16_001436"
     CDS             complement(1694..3136)
                     /gene="gtfA"
                     /locus_tag="VVN16_001436"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU5572809.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            complement(3413..3493)
                     /locus_tag="VVN16_001437"
                     /pseudo
     CDS             complement(3413..3493)
                     /locus_tag="VVN16_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3494..4327)
                     /locus_tag="VVN16_001438"
     CDS             complement(3494..4327)
                     /locus_tag="VVN16_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5572810.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4341..5207)
                     /locus_tag="VVN16_001439"
     CDS             complement(4341..5207)
                     /locus_tag="VVN16_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5572811.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNVLHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5221..6480)
                     /locus_tag="VVN16_001440"
     CDS             complement(5221..6480)
                     /locus_tag="VVN16_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5572812.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYCKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6572..8734)
                     /locus_tag="VVN16_001441"
     CDS             complement(6572..8734)
                     /locus_tag="VVN16_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU5572813.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLFYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            8842..9702
                     /locus_tag="VVN16_001442"
     CDS             8842..9702
                     /locus_tag="VVN16_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5572814.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            9699..10634
                     /locus_tag="VVN16_001443"
     CDS             9699..10634
                     /locus_tag="VVN16_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU5572815.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(10760..10847)
                     /locus_tag="VVN16_001444"
     tRNA            complement(10760..10847)
                     /locus_tag="VVN16_001444"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10811..10813),aa:Ser,seq:gct)
     gene            complement(10854..10927)
                     /locus_tag="VVN16_001445"
     tRNA            complement(10854..10927)
                     /locus_tag="VVN16_001445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10891..10893),aa:Ile,seq:gat)
     gene            complement(10963..11033)
                     /locus_tag="VVN16_001446"
     tRNA            complement(10963..11033)
                     /locus_tag="VVN16_001446"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10999..11001),aa:Gly,seq:tcc)
     gene            complement(11053..11125)
                     /locus_tag="VVN16_001447"
     tRNA            complement(11053..11125)
                     /locus_tag="VVN16_001447"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11090..11092),aa:Phe,seq:gaa)
     gene            complement(11129..11202)
                     /locus_tag="VVN16_001448"
     tRNA            complement(11129..11202)
                     /locus_tag="VVN16_001448"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11166..11168),aa:Met,seq:cat)
     gene            complement(11216..11305)
                     /locus_tag="VVN16_001449"
     tRNA            complement(11216..11305)
                     /locus_tag="VVN16_001449"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11267..11269),aa:Ser,seq:tga)
     gene            complement(11316..11389)
                     /locus_tag="VVN16_001450"
     tRNA            complement(11316..11389)
                     /locus_tag="VVN16_001450"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11353..11355),aa:Ile,seq:cat)
     gene            complement(11403..11476)
                     /locus_tag="VVN16_001451"
     tRNA            complement(11403..11476)
                     /locus_tag="VVN16_001451"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11440..11442),aa:Met,seq:cat)
     gene            complement(11482..11555)
                     /locus_tag="VVN16_001452"
     tRNA            complement(11482..11555)
                     /locus_tag="VVN16_001452"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11519..11521),aa:Pro,seq:tgg)
     gene            complement(11571..11644)
                     /locus_tag="VVN16_001453"
     tRNA            complement(11571..11644)
                     /locus_tag="VVN16_001453"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11608..11610),aa:Arg,seq:acg)
     gene            complement(11654..11739)
                     /locus_tag="VVN16_001454"
     tRNA            complement(11654..11739)
                     /locus_tag="VVN16_001454"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11703..11705),aa:Leu,seq:taa)
     gene            complement(11747..11818)
                     /locus_tag="VVN16_001455"
     tRNA            complement(11747..11818)
                     /locus_tag="VVN16_001455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11784..11786),aa:Gly,seq:gcc)
     gene            complement(11841..11913)
                     /locus_tag="VVN16_001456"
     tRNA            complement(11841..11913)
                     /locus_tag="VVN16_001456"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11878..11880),aa:Thr,seq:tgt)
     gene            complement(11932..12013)
                     /locus_tag="VVN16_001457"
     tRNA            complement(11932..12013)
                     /locus_tag="VVN16_001457"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11977..11979),aa:Leu,seq:tag)
     gene            complement(12019..12091)
                     /locus_tag="VVN16_001458"
     tRNA            complement(12019..12091)
                     /locus_tag="VVN16_001458"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12056..12058),aa:Lys,seq:ttt)
     gene            complement(12116..12188)
                     /locus_tag="VVN16_001459"
     tRNA            complement(12116..12188)
                     /locus_tag="VVN16_001459"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12153..12155),aa:Asp,seq:gtc)
BASE COUNT         3869 a         2689 c         2172 g         3459 t
ORIGIN      
        1 cacaccaacc ttattccgaa ttcaattact tttgtgattt acatatatag attaagacta
       61 tcttttatac tttaaaattt ctcgctacct tatccactat atgctcctcg ctatcacgtt
      121 tctattcata gcctacgatt tcactattgc tttctctgac aattcttatt tcctgcgtca
      181 gacttaaaac gatctatccc cagaccattt taatccgcta cctcacgata gtcaggcttg
      241 gggagtgcta ttgtattcac cggtagtgga tccctacaga ggacttacac ctcagatgca
      301 cgacatgccc atcgtataaa aaaatctcct acccaaggta gaagatttca aacttataaa
      361 acttaatccg tcatgtccga taccaacatt cgatgctcca atggaatact gcacataact
      421 agcaagaaaa taaagcctga ctgaatccag aagagagcca agtcaaaaat tccgtgcaca
      481 gcaaccactg taaggaaaga tagataaagg ccgataatcg gacgtttccc cgactcctga
      541 ctcatatcca tcatcaagcg aacaggagca acagaagaca aaactaataa aatggtaccc
      601 acaattccgt aactcagaat cgtatcaata taaagactgt gggcatgttc atgataagga
      661 gcatgtatcc gaggataaga gtgcatatag gtcaatggcc cttcacccca aaaaggattt
      721 tgcttaaaca aggccatccc agcatcccag atagaaatgc gttcttccat agaagagtct
      781 aaagtaccca ttcgaactcc caaatcacta gaaaagagga aactcaaacc aatcgcgaag
      841 accccaatac taagccaaaa ggccttccag tttttaatag tcgtaaagag atagataatt
      901 gctccagcga taatagcagg aaaggcagtt cgattttgag taaagttcaa accaaagaga
      961 ttaacaaagc ctgcaatcac acagaatact ttcaaccaat tcaacttggt cgttgtaaac
     1021 agatagaaag caatcataat acagaaacaa caaataattc cataataatt aggattaaag
     1081 aaggtcactt ctgcccggtt ctgatgccac acctgcatat tgggtgaaag aaaagcatag
     1141 ttaaatttct tcacaatttg gaaatgttct aaactggcaa aagcagctga caagacacta
     1201 ccaaacaaga cgaactgcaa aatcaatcga aagaatttat gggataaaat cgactgatag
     1261 tgcaaaaaga aaatagtaaa tagaaacatt cctactgaag ccacaagacc catccaattt
     1321 tgtgcaagaa tggatataac agtactatag ctaagaaaaa gaagcagcat cggatgctcc
     1381 cccattttct gaagaatact tttcatgtct cctgtaaaaa tcaaactgat aatatataaa
     1441 cagagtacaa ctacaaaaag ataaaagggt aaaaagatac ttaggataat tcccaataaa
     1501 atcagctctt tactagacaa ccccttcagc ttttcaataa agcctattga tttcaaaatg
     1561 aatcctttct ctccaaatca gctgattcag ataatagtaa gctatcctat attgtaccac
     1621 ttttttagca atttgaaaac aaaggaaacg ttttccaaaa taaaaaccct attttatcca
     1681 ccatatcaag gcttcaaaat gttacttcaa ctccattctc aattacccga taagtctgat
     1741 tttgcaaatc aatttctgct actgctgtta cggacttatc tttattttga cgtttgatta
     1801 caatgctgtg agctgttggt gtctctatct cagtagtccc ttctagatca aaggcttctg
     1861 aacggttacg gaaagaaaat agattgagaa gggccttcac aacagatcgt tgcacttctt
     1921 ttgctatttc ctcgttgcta tagtaatgac gattaatatt tcgaccttct ttagtttctt
     1981 ctaataattt caagtcattc ttgcctgcta atagacccac atagtaaacc tgaggaatac
     2041 ctggggcaaa agcttgaatt agacgagcga gaaaatactt gacatcatca tctccaagcg
     2101 ctgaatagta ggttgaattg atttggtaga tatctaagtt gttatactcg gcactagagt
     2161 acttacgttt gacattggct ccaaccttat agagttcatt tgaagcatag tcaatctcct
     2221 catcggtcag gatatccttg acatctacta ctccaatccc atcatgggta tctagcgtcg
     2281 taaattgctt catcgggctc atctttaacc acttagccaa acgctctgtt ctggaactgt
     2341 aaagagtata aagtgtcacc attggaagag caaaatcata aacatagtaa tcatggtctg
     2401 ctattttaaa ctgaatcgaa tagtgttcat gaatctcagg taaaagctct gtcccatact
     2461 cagcagcgat atctcgaact ttgtccaata aatcccaaat atctggttcc acaaagaaat
     2521 cattagtatc caatttcttc actgcataag caaaggcatc tagacgaatc aaatcacacc
     2581 cattacttgc caagtgctga atggtcttac ggataaattc catagttact tctttggtca
     2641 catcaagatc aatctgctcc tcaccaaagg tattccacaa atgttccact gaaccatctt
     2701 caaacacaat ctcttgcttt ggtgcacgat ccttacgctt gtaaattaaa tctacatcag
     2761 actgtgtcgg acggttttct ggccaaaact tatcccagtt taaaaagaga tctttaaatt
     2821 cactggcttc atgtttttct tgatagtcct tataatactt ggattgacga gaaatatgat
     2881 taatcataaa atcaaacata agataatatc tctcacctaa acgcttaaca tcctcccaat
     2941 caccaaaagc tgagtccact tcgtcgtagt caactggcgc aaatccacga tcacctgttg
     3001 atgggaaaaa tggtaaaagg tgaactcctc caatagcatc tccaaaatgc tcttccaaat
     3061 tatcatataa gtctttaaga ttatttccaa ggctatcaga ataggtaatc aacatggttt
     3121 tattttgaat tggcatcatt actctccttt ttctaattga agccaagtct catatgatct
     3181 ggcttcataa ataaaattca ttttaaatct ctatttatca tcaaactcgt actaatatag
     3241 actgtgataa acaaagtact actttcttgt tttctacata gaattatcaa caagctgaac
     3301 tcttcctctg tgtcaaagac tatagattcc atgagctctt cttatactat tcgaaaatct
     3361 cttcaaacca cgtcagcttc accttaccgt aggtatggtt actgacttcg tcagtttcat
     3421 ccacaacctc aaaacagtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga
     3481 ttttcattga gtattacttc actgccccgt tgctcattcc tgaaatgata tggcgttgga
     3541 agaagagata gacaatggtg atactgataa tgccgaccac gtaagaggca aagcttggtc
     3601 cgtagtcgtt gaaatattgg cctgcgtagt tgtattggaa caaaggcaga gtccacattt
     3661 tggaatcccg gttcaagaca aggagtggca acatgaagtc attccagaac caaagggcat
     3721 tgatgatcat ggttgtcgca tgcatcggtt tcatcattgg gaagatgatg cggaaatagg
     3781 ttgtaaattg attagcccca tcgatctctg ctgcttcatc cagactttct ggaatcgaga
     3841 ttttgatata gccaacatag agaaagaggg tctgtggaat cgcataggtc aagtagagta
     3901 agatcaaacc aaaggtatta gccaaaccga gtttactcat cataaccgta atcggaatca
     3961 tgatgacttg gaaaggtacg aagattccga ggattaagag ggtatacatg atggtaaagg
     4021 cttttctttt actcatattg cgagcgatgg agtaggctgc cataggtata aagatcatta
     4081 ctgcaagtaa agacaagaca gtgatgacga cagagttcca ataatagcct ccaatcccat
     4141 cagctaagag acggctaaag ttgtcccatg tgaagttggt tggaaagcca aagaaattat
     4201 ctacaatatc cttagtgggt ttgaaggaac taaagagggt agcaaggagc ggcactaaaa
     4261 tcagaaccga tcctagaatc aatagaatgt atttgccaat cagggctttt ctttcatctt
     4321 gtttcatcat gcttctcctc ttaaatttca aatttcttag atactctcaa ttggatgatc
     4381 gaaatcacta caattaagaa gaacaagatt acggcaatgg cattggcata accgaattgg
     4441 ttgtttttaa aggcatagtt ataaaccaag agcccaagtg aggttgtggc attgtttgga
     4501 ccaccaccgg tcatggcaaa gacttggtca aaggcagtca gcccaccttt tagggctagg
     4561 ataaagacca tagagacact tggtagcaag taaggcaatt caatgttcca gaaaacttgc
     4621 ttgctcgtcg caccatcaat ccttgctgcc tctgtaatct cagttggaat agattgcaaa
     4681 ccagctagga agatgatgat gggcatagcc accccttgcc aaagaaggac aaagacagcc
     4741 gcaaagattg ctccccactt agtccctaaa agactggttt ggagaaattc aatatgaagg
     4801 acattcccaa tcgctggaag accgtagttg aagacttgct tgaagatcaa agccactgtc
     4861 aaaccagata aaacagctgg gaagaagaac caagcacgga agaaggtttg gcctttgatt
     4921 ttagaattca agacacgcgc aatgaagatc ccgagtgcaa tctcaccaac caccatggca
     4981 atcgtaatga ttgcggtaaa gccaatcgca ttcatgaatt ttggatccat gaagaggagc
     5041 ttaaagttgt ttaagccgac aaatttgtag ttataagtca atcctgtcca gttggtaaaa
     5101 ctgtaaaagg ctccttgaaa catcggcaca tagaagaaaa ttgcttgtaa caagaggggg
     5161 atgaccacaa aagcccatgc ccaatatttt tgtaatactt ttttcatagt ctctctactc
     5221 ctaatccaca tccgctttca tcgggttaaa gaaggcattc aaatcattga ccatgccttg
     5281 tttatcaccg gtcaagacat agttcatggt caaggtatgg aagtctgctt cactggtcca
     5341 gtattgttgc aaccagacca agtgacgatc cgtaaaggca tattcggtca taccagcaag
     5401 cggtgaatct tctcctgctt gtttgactcc ttcgatcgct gttggagatc cgtccacatc
     5461 gtagtatttt tgcatgactt ctggacgggt catatattcc acaaaggcat tggcttcttt
     5521 tggatgtttg gtggtggctg agatagacca tgccaagtct cccgcaccaa cggttaagct
     5581 ttgtcctttt tcttttcctg gaatcatgaa ggtcccaatc ttaaagttcg gtttttgttc
     5641 attaatcgct gtgatcgccc aagacccatt tggtgtcatg aggacatccc cacgtgcgaa
     5701 ggctccgata acatcggtat agccagcacc ttcccagttc ttttgcttag agccattgat
     5761 gcgaaggatg tccatgacct tgatatcatc tttcataatc ggatccgaca atttaatggc
     5821 atttggttga gaataacgaa ggtattgatt tgcttctttt cctccacctg ttgctgtcgc
     5881 aaaggctaat tgattgtaac cattgagtgt ccaagcatct gcacctgcaa ttccaaatgg
     5941 tgtttgtcct ttagcaacga tatctttgac tagctgttca aattcatccc aggtttcagg
     6001 aaccttcaag cccagttctt cgaatttatc tttgttgtag taaattccat aagcattagc
     6061 tgtaaaagga acgttgtaaa ctttttcgtt tacagcatat ttttcagcgt agccattttt
     6121 cacgcgtttc aggtagtctt tgttgctcaa atcttcaaaa acacctgctt ttgcccattc
     6181 ttgcagttcg atggactgtg ggtaaatatt gaccacatca ggcacatctc ctgcgagaac
     6241 gcgtgtcttc aatacttcac cagcatttgg tacattgacg actttgacct tgatcttagg
     6301 gttttccttc tcaaaatcac gagtgatttc ttccaaggtt ttggtcattt cttttttctg
     6361 gttgaaatac tcgatggtca ctgtgccatc cgcagattta caatagttgg agcaagcgcc
     6421 gagcccaaac aaagctaaac ctgtagttgc aagaagtccg atttttttat accattccat
     6481 tagaaagcct cctttataaa tttatacacc cttattgaac tgcaccccaa aagttagaca
     6541 gaataaatct aacttttggg gtcagtacat atcatagttt tctaaaaata tactgtctac
     6601 tcaaaaaatc tccttgggat aagataacag ttaagcccgc atacattagt tctgcacctg
     6661 agtaaacttc gccattttcc tgtaatttat atagtccctc ttcatccaaa tcttttaatt
     6721 ttaaagttgt ttccatggtc tctacaacag ataaaacgcg aacgtaggtt acaatcgttt
     6781 gatttccgta attaaattgt acagctgctt cattggatac agtatcagga ttaattagtc
     6841 tatactgctg tcctaactga actactggtc gtaattcttt atacaagttc acctgattag
     6901 caatcgtagc tttctcttca tctgataaat ttgtcaaatc aagttcatag cccaaatttc
     6961 ccatcattgc tacaaggcca cgtgtttcta atggtgtcat tcgtcccatc tgatgattcg
     7021 gtactgctga cacatgagcc cccatagaaa tggttggata gagataggat gaaccgtatt
     7081 gaattggtaa acgtgcaatg gcatcagtat tatcactagc ccagacttgt gggaaatagc
     7141 gcatcatacc aagatcattt cgtccaccac caccagagca ggactcaaag agaatatggc
     7201 tgtgcttctc tgtcagataa gaaacgagtt cataaagccc cagcatgtac tgatgagatt
     7261 gcatctgtgt ctctagataa gttaatccat tccctagctt agtgatattg cggttcatat
     7321 cccatttaat gtaatcaata tcatgataaa ataggagttg atctaagaca cttttcaagt
     7381 attctactac ctgaggattg gcaagattaa gtactaattg attccgagaa taagtatgct
     7441 catagccagg aacctgaata gcccagtcag gatgttgacg atacaaatca ctatctacag
     7501 aaatcatttc gggttctaac caaagtccaa actgcaaacc tctttcatgg atagctgaaa
     7561 tcagactttc tagacttcca cccagttttt cctcattaac aacccaatca cctaaagcac
     7621 gattatcatc aaaacgattg ccaaaccaac catcatctaa tacaaaaagt tcaatgccaa
     7681 ctttcttagc ttcatctgct aactctaaca gtttttctct ctgaaagtca aagtaagtag
     7741 cttcccagtt attgattaga attggacgtt cttttttaga aaattcactt agcataatgt
     7801 gcttcagtac aaaattctga ctttcatgac taataccagt taatccctga tctgaatgag
     7861 tcactaaagc taccggtgtt tcaaagtatt cctcaggagc taacttccaa gaaaagtttt
     7921 ctggattaat gccaatagcc acccgaactt cattcaattg atttttttga acaaaagctt
     7981 caaagttgcc actatacatt agttgaatag caaacacatt cccagcatcc tctgtgactc
     8041 cttgttcgca tagtagaaga gctggtgttt gagcatgacc agaagcacct cggtttgaac
     8101 taatcgaaaa gattccttgt tctacctgtt gacgtctaac agtcttttca cgagcataag
     8161 caccctgcag agttactatt tcgtaatctg cagctggaaa atcagccata aaagaaaaat
     8221 ctttatggat gacaacttcc tgattactat tattatctaa tttactgtag ctagcaatag
     8281 tcgcatcatt attaaaagta gtataataca aagtcagact aagttgagcc ttagaatctt
     8341 ctaacattaa gacaagagtc tctgtatcgt ccatgctatg tggagaaggt aagccctgtg
     8401 gaccattctg accttttaaa atctttgctt ctacaaatcg aaagtctgtt acttcagtta
     8461 cattatgctg aacctgtatg gttggtttcc taaaatctcc taagccatgt tgtccaaaaa
     8521 tttgacgttg agtatctaaa ctaaaggttc gattagtagc tgttggattt cctgaaaagg
     8581 catggtctcg ttcataaaca ctattggaac ctttatagtt cttaatagtc tttcctaaat
     8641 gtttcaaaag taagtagcca tttcgatttt caataatcaa acttagattt ttactctcaa
     8701 cataaaatag attattctct atcctaactc ccatttactt cacctcatca ctttattgat
     8761 tatattttat cacctgaaat cgctttctaa aatagaaaaa tgtctcaaga atatggtaaa
     8821 atgttaggta ggaggtagca catgttagtt ttttcagaat accagactgg aacaatcgac
     8881 cttgccctaa gcttttatgg atatgaggaa tgcacaccta attactcttt tggtccagcc
     8941 attcgtgata catacgttct acattacatt actaaaggac aaggaaaatt ttattacaag
     9001 ggtaaaattg ttgatttaaa agaaggagat ttctttctat taaaaccaga ggaactaacc
     9061 ttttatcaag cagatagtaa agaaccttgg gcctactact ggttaggaat cactggaggg
     9121 aaatcccctg attattttgc tctttctcaa atttctgatc aatcctatct catccaatct
     9181 gaaacttgtc atacccagac tactgcaaaa ctcatctcag acattgtccg cttcgctcag
     9241 attacaaaat caagtgaatt agctcaactc catatcatgg gacaacttca tgaactgatg
     9301 tttcatctgg gaactattgc tcccaatcag aaaaaaaaga atatttcatc aacccaccaa
     9361 ctctatcttg aatgcaaacg attaattgat agccactatc ctcaatcact tacaattcaa
     9421 gatttagcaa aagaactatc cgttcacaga agctacttat caagcgtatt caaagaattt
     9481 aataccttat cacccaaaga atacctactc tacgttcgaa tgcaccgagc tagacaactt
     9541 ctcgaaaata cccaagagtc catcaaggta attgcatact cggtaggttt ttcagatcca
     9601 ctccattttt cgaaagctta taaacaatac tttaatcaga ctccaagtca tacaagaaaa
     9661 gaatactctc aataccaact agtgagaaag gcaacattat gaaatcctac caagctgtct
     9721 accaaatcct atctaaagaa accgactata tcagcggaga aaaaatcgca gaaaaactat
     9781 ccctaagccg aacatcaatt tggaaagcta tcaagcgact agaacaagaa ggcattgaaa
     9841 ttgatagtat caaaaataga ggatataaac tgatgaatgg tgaccttatt cttccagaga
     9901 ttctagaaga aaatcttcca attaaagtca gctttaaacc cgaaacaaaa tcaacacaac
     9961 tagatgcaaa agaagcaatt gatttaggcc atgaagcaaa taccctctat ctagcttcct
    10021 atcaaacagc aggccgaggc cgttttcaac gttccttcta ctcaccacaa ggtggtattt
    10081 atatgacact ccatcttaaa ccaaatctcc cctatgacaa attaccatcc tacacactac
    10141 ttgtagctgg agctgtctac aaagctatta agaacctaac tttaatagat gtcgacataa
    10201 aatgggtcaa tgatatctat ctaaacaatc ataaaattgg aggaatcctt actgaagcaa
    10261 tgacctctgt agaaactggc ttagtcacag atatcattat tggagtaggt atcaatttca
    10321 ctattaaaga cttccctcag gaattaaaag aaaaagctgc cagcttattt aaagctacag
    10381 ctcctataac aaggaatgaa ttgatcatag aaatctggcg tgctttcttc gaaacaccag
    10441 cagaagagct attataccta tacaaaaaac agtcattcat tctaggaaaa gaagtcactt
    10501 tcacactaga gcaaaaagac tacaagggac ttgctaaaga catctcagaa aatggaaaac
    10561 ttttagttca atgtgataac ggaaaagaaa tctggctaaa tagtggcgaa atttctctca
    10621 atagttggaa gtaaaataac acaattataa tataaacgat ataaaaataa cttcagatta
    10681 gtaattcaat taagttttac ggatctgaag ttttattggc tctaaaaaca aaaaagagag
    10741 ttacaaactc tcattaaaac ggagaataag ggattcgaac ccttgcgcca gttacccgac
    10801 ctaacgattt agcaaaccgt cctcttcagc ctcttgagta attctccaat taatgggcac
    10861 gagtggactc gaaccaccga cctcacgctt atcaggcgtg cgctctaacc acctgagcta
    10921 cgcgcccaag ttaaaaaact tggtaatttg aacaaagttc aaagcgggtg acgagaatcg
    10981 aactcgcgac aacagcttgg aaggctgtag ttttaccact aaactacacc cgcataaata
    11041 ctatcaataa aatggcgcga gacggaatcg aaccgccgac acatggagct tcaatccatt
    11101 gctctaccaa ctgagctacc gagccttatt gcgggagcag gatttgaacc tacgaccttc
    11161 gggttatgag cccgacgagc taccgagctg ctccatcccg cgttaataat ataaaaggag
    11221 gatgtgggat tcgaacccac gcacgctttt acacgcctga cggttttcaa gaccgttccc
    11281 ttcagccgga cttgggtaat cctccaatat tcaaatggac cttgtaggac ttgaacctac
    11341 gaccactcgg ttatgagccg agagctctaa ccagctgagc taaaggtccg acaagatcat
    11401 tatagcggcg aaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca
    11461 gctgagctac accgccatga atcgggaaga caggattcga acctgcgaca ccttggtccc
    11521 aaaccaagta ctctaccaag ctgagctact tcccgagtta aatagaaaaa tgcaccctag
    11581 aggagtcgaa cctctaaccg cctgattcgt agtcaggtac tctatccagt tgagctaagg
    11641 gtgctccata ttatgccgag gaccggaatc gaaccggtac gatcgttacc aatcgcagga
    11701 ttttaagtcc tgtgcgtctg ccagttccgc caccccggcc tctctaagcg aacgacggga
    11761 ttcgaacccg cgacccccac cttggcaagg tggtgttcta ccactgaact acgttcgcac
    11821 tgttttcttc tatctaaaaa tgccggctac atgacttgaa cacgcgaccc tctgattaca
    11881 aatcagatgc tctaccaact gagctaagcc ggctcatttg ttatatctta atgcgggtta
    11941 agggacttga acccccacgc cgttaagcgc cagatcctaa atctggtgcg tctgccaatt
    12001 ccgccaaacc cgcatatatg acccgtactg ggctcgaacc agtgacccat tgattaaaag
    12061 tcaattgctc taccaactga gctaacgagt ctaaaataac ttgcgttacc ttaaacggtc
    12121 ccgacgggaa tcgaacccgc gatcttcgcc gtgacagggc gacgtgataa ccgctacact
    12181 acgggacct
//
LOCUS       DAVHIT010000057        12012 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVHIT010000057 DAVHIT010000000
VERSION     DAVHIT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.058"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(121..192)
                     /locus_tag="VVN16_001460"
     tRNA            complement(121..192)
                     /locus_tag="VVN16_001460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(157..159),aa:Arg,seq:cct)
     gene            complement(234..581)
                     /gene="rplS"
                     /locus_tag="VVN16_001461"
     CDS             complement(234..581)
                     /gene="rplS"
                     /locus_tag="VVN16_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5572816.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(700..1029)
                     /gene="crcB"
                     /locus_tag="VVN16_001462"
     CDS             complement(700..1029)
                     /gene="crcB"
                     /locus_tag="VVN16_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5572817.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1023..1397)
                     /gene="crcB"
                     /locus_tag="VVN16_001463"
     CDS             complement(1023..1397)
                     /gene="crcB"
                     /locus_tag="VVN16_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5572818.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1394..1660)
                     /locus_tag="VVN16_001464"
     CDS             complement(1394..1660)
                     /locus_tag="VVN16_001464"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU5572819.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKCSRDYQDQNIK"
     gene            complement(1776..2219)
                     /locus_tag="VVN16_001465"
     CDS             complement(1776..2219)
                     /locus_tag="VVN16_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU5572820.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2323..3258
                     /locus_tag="VVN16_001466"
     CDS             2323..3258
                     /locus_tag="VVN16_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU5572821.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3312..3596
                     /locus_tag="VVN16_001467"
     CDS             3312..3596
                     /locus_tag="VVN16_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU5572822.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3653..3871)
                     /locus_tag="VVN16_001468"
     CDS             complement(3653..3871)
                     /locus_tag="VVN16_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572823.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRYNS
                     IAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3868..4442)
                     /locus_tag="VVN16_001469"
                     /pseudo
     CDS             complement(3868..4442)
                     /locus_tag="VVN16_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4717..4977
                     /locus_tag="VVN16_001470"
     CDS             4717..4977
                     /locus_tag="VVN16_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572824.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            4931..5152
                     /locus_tag="VVN16_001471"
     CDS             4931..5152
                     /locus_tag="VVN16_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572825.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            5471..6400
                     /locus_tag="VVN16_001472"
     CDS             5471..6400
                     /locus_tag="VVN16_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5572826.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            6414..7337
                     /locus_tag="VVN16_001473"
     CDS             6414..7337
                     /locus_tag="VVN16_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5572827.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            7595..9070
                     /locus_tag="VVN16_001474"
     CDS             7595..9070
                     /locus_tag="VVN16_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU5572828.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            9342..10328
                     /locus_tag="VVN16_001475"
     CDS             9342..10328
                     /locus_tag="VVN16_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU5572829.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            10603..11463
                     /locus_tag="VVN16_001476"
     CDS             10603..11463
                     /locus_tag="VVN16_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU5572830.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
BASE COUNT         3743 a         2413 c         2311 g         3545 t
ORIGIN      
        1 gattgcccaa atttttcaat aataaaacgc ataatattat ggagacacac aaaatcacct
       61 atattatgcg ttcttttaat atcaaagact ttttgacaaa cttctttgat gtctaattac
      121 tgccccctgc aggaatcgaa cctgcaacta ctccttagga gggagttgtt atatccattg
      181 aactaaggga gctagataaa aactctgctg aaaagcagag ttttttagtc gaattaacga
      241 cgaatttctt tgatacgagc tgctttacct tgaagagcac gcaagtagta caatttcgca
      301 cgacgtactt taccgtaacg aacaacttcg attttttcaa cacgtggagt gtggattggg
      361 aagatacgct caacacctac accgttagag attttacgaa ctgtgtagtt ttctgagatt
      421 ccagcacctt tacgtgcgat aacaacacct tcaaaaatct ggatacgttc acggttacct
      481 tcgacaactt tcgcatgtac acgaacagtg tcaccaggac ggaatgatgg gatatctgta
      541 cgaagttgac cttcagtcaa gctttggatt aatggattca ttttattctc ctatctttgt
      601 caatcttgag gaatcttcct cagcggataa actgtatttt tgtgcgtcca ttacacacaa
      661 gatatagttt accaaatttc taaaaaaaag taaagaaatt tatagcaaaa ttcctaaaaa
      721 aatcgcaacc aaaccaccta tgtaagtcaa agtcaggtaa gaataaaaga ccttcttatc
      781 acttagcagt ctttgaagtt cgtcattcaa ggtcgaaaaa gttgttaaac cgccacaaaa
      841 tcctgttgct agaatagcat atacttcctt agactctaca tgattgtaga atactccaat
      901 taaaaaacaa cctaaaagat tagctatgag cgttccgagt ggcaatttag aagcttgatt
      961 atagcgagaa aagaaatacc gtacgagtgc tcccaagcca caggcgattg caagatagac
     1021 gattaccatt tcttcctccc caaatagtaa gctaaaagca ggcctccacc gatactcaaa
     1081 agcaaataca gtatcaaact aagataacgc cctgtatcaa gcagcttcac agtatcaagc
     1141 attaggctgg aaaaagttgt taaacctccg caaaaacccg tccccagtgc taaaatcaag
     1201 cccttactag tccccttata gaccagatag cccttgacaa gataaatcaa gcagaaaatt
     1261 cccagatagt tgacaaagag agtgccccaa ggaaagtctg gactggctgg taaccaggtg
     1321 gaaactagat atcggacaag tccgcccaac atagcagcta gaaaaattcc tagcggataa
     1381 aattgttctt ttttcatttg atattttgat cctgataatc acgcgaacat ttgagtatgt
     1441 ccgaaaaagt cgcgacaata gtctcctgat agcgcttgtc ctctacacga cttctaacct
     1501 tttcaaaaat aactgcttct ctcttagtat ctaaaatcgg tttccctgaa gccttcttat
     1561 aagcgacaac tccttcaact aagtgcattc gttcttctaa cagtttgaca atttggtcgt
     1621 cgatttgatc gatttcttga cgaataatat ctaaatccat acagtctcct cctttatttg
     1681 aattattgta tcaaaaagcc atcaattagg ctagtaaaat cccaactctc gataagaaaa
     1741 atgcactgat tgattacatc agcgcacttt tatgcttatc ctactttagc aaccaattct
     1801 tctgcgaatt gttccaagcg ttcaatatct tcttcctcgg cagaaagatc aactttaaca
     1861 cactctgaac ctttttctgc tcctgttgac acaaagacac ggtcaaagtc atcaacagcc
     1921 ttacagaatt catcgtagaa ggtatctcct gagccgacca ctccgtagat tttgccattc
     1981 aagttgagat ctgctaggtc ttcgtagaag tccatcatct catctggcaa ttcaccatca
     2041 ccataagtat aggtcgcaac gatagcgatg tctgcttcca agaagtctga agcgtcaaca
     2101 gttgtacatt catcaacatc aacatccaag cccaagtcac gtaatttgtc tgctacaata
     2161 tctgcaattt cttcggtatt accggtcata ctggcaaata caatttttgc taatgccata
     2221 tcgtcctcct caatttatct ttctccatta tatcacatct ttttcatttt aaaaataaaa
     2281 attcttgtgc tacaatgaaa tgagaaagaa ttgaggttat ttatggagat ttgccaacaa
     2341 attttagaaa aaattaaaga atacgacacc attatcattc atcgtcatat gaaaccagac
     2401 cctgatgcct tgggaagtca ggtgggattg aaagccttgc tagaacatca tttcccagaa
     2461 aaaaccatca aagccgtcgg ttttgatgaa ccaactctta cttggatggc tgagatggat
     2521 cttgttgaag atagagccta ccaaggcgca cttgtcatcg tctgtgatac agctaatact
     2581 gctcgtatcg atgataagcg ctatagtcaa ggtgattttc tcattaagat tgaccaccat
     2641 ccaaatgatg atgtatacgg tgacctgtct tgggtcgata ctagttcaag tagcgctagc
     2701 gagatgatta ccctatttgc ccaaacaacc caactagcct tggcagatcg cgatgctgag
     2761 ttgctctttg caggaattgt cggtgataca ggtcgcttcc tctacccttc taccactgca
     2821 cggactcttc gcctggctgc ttatttgaga gaacataact ttgactttgc ggctctcact
     2881 cgcaaaatgg acactatgag ctacaaaatt gctaaactgc aaggctacat ctacgaccat
     2941 ctggaagtgg atgaaaatgg tgctgctcgc gttatcctga gtcaagaagt cttgaaacaa
     3001 tacaatgtaa ccgatgctga aactgcagcc attgtcggtg cacctggacg cattgacaga
     3061 gtgagtctct ggggaatttt tgtcgaacag gctgatggcc actaccgagt tcgcttacgc
     3121 agtaaaatcc atcctatcaa tgaaatcgcc aaggagcatg atggtggagg ccaccctcta
     3181 gcaagtggtg ctaattccta tagcctagaa gaaaacgaaa tcatctacca aaagttaaaa
     3241 aacttgctta aaaactgata aaatacttgc caaacttttc agaatctgat agactagtat
     3301 ggtaacaatc tatggctcgc aaagagacca tggcagaaag gaaatattgc aaaatgaaaa
     3361 aagatatcca tccagaatat cgcccagttg tcttcatgga cacaactact ggttaccaat
     3421 tccttagcgg ttcaacaaaa cgctctaacg aaacagttga gttcgaaggc gaaacttacc
     3481 cattgatccg tgtggaaatt tcatcagact cacacccatt ctacactgga cgtcaaaagt
     3541 tcactcaagc agatggacgc gtggatcgtt tcaacaaaaa atacggtctc aaataatgat
     3601 aagagaacag tttcggctgt tcttttttgt ttcttgaaat caactgctgt tttcatgttc
     3661 cagactcatc tgtaggttcg gtttccatgc tactaggcag aaaggaaata gctgtttcaa
     3721 cacgtccata atgagctata ctattgtaac gaaccacact ttcattgatg gtccaagtgg
     3781 aattcatttt cttaaaagct tctcggactt tttccaaatc tttggaggca atggcctgct
     3841 ctaaggtttc aaaacgagga cttatactca tctgctttca caaagcattc tagtccatct
     3901 ccgattaacg atggacttta tcacctcctt ctccagtcct tgtatgacat cttgaagttg
     3961 attcatgata tcttccaaag ttcgaaaggc tttattctta aatccacgtt tacgaatctc
     4021 tttccacact tgttcaatgg gttcatctct ggtgtgtatg gaggaataaa ggtaaaatca
     4081 atattagtcg gaatatttaa ggtacttgat ttatgccata tagcattgtc cataacgagt
     4141 aaaaggataa gcttgtgaaa gctcttctaa aaaggcgttc atccacactc ctttttagaa
     4201 gcctgaaata aggcatcaat tgtaacaaat tctcctgcct ctgcagcctt caaatggcgg
     4261 gcaagaaaga ctttctcttc ctcaactgtc atatatgcat ggttacgacc accacgtgtt
     4321 tcttgaagga gagagtcgag tccgaactcc tcatattttt ttacatttcg ccaaatcgtt
     4381 gtttgattac agtctaaaag ctctataatc tctttataag atttgcccat cagacgaaat
     4441 atagtagatt gaaactagaa tagtacacct ctacttctaa aacattgtta gaaatcgatt
     4501 tgactgtcct gatcgatttg tcctgttctt gtttcatttt actatagaac gatttgaagg
     4561 cgtttatgat atttagctgt acgagagtct tttaaaagtg ttttgatggt ttggatttct
     4621 tctttagttg atttcatatc actgttatat aatgcttttt gatttgagtc tagtattagt
     4681 aagcagactc aaaccttaga aaatcttttg agatcattga caggtgtatc gaatcctacc
     4741 acagattcag cacgtctggt tttggcagag gctaagaaag ctcttgcaga tgatagtttg
     4801 acagagcaag gtttacgtga tatcttgcaa acagtcaaag atgccattgc ttccctagag
     4861 tccattaagg aaagccagtc agcaactaag gatggaggac aaacagcggg caaagaaaca
     4921 gcaggagaag atgagtctgc aaatcaaact caagaaatta gccaaggaat taagtaaact
     4981 tcttaaagac tcaaaccttg aaacagtaga taaggatgtc cttgaaaact ctcaagaaga
     5041 acttcaaaaa gcagttttgt tcttggcaga tgaaaaaggc tctgagcata ctgcagcaga
     5101 gttgattgat aatcttaaag aggttattgc taagttgaaa gcaaacgcct aataacatat
     5161 taaaaatctc cagattagga actatcgctg agttccctag tctggagatt tttcagtata
     5221 ccccgttgtt ggatgcttat aacagagtga cgccatcact gggctaatag agataaaata
     5281 aacaattgat ttaggacatt tgtttgatag tggtgataaa gttttaaatc tgctacataa
     5341 tttgtggagt tttttctata attaggagtt taacctaacc tgcaaatagg agtatactaa
     5401 taatgtaatc gttatcaaaa gtctaaaaaa gaatttttag atggatatca aaataaaaag
     5461 ggaggaaatt atgaaaaagt tttcaaaaac attgagagac aactggatct ttctcttgat
     5521 ggttttgcca ggggcactct ggttgattct attcttttac attccagtat ttgggaacgt
     5581 ggttgccttc aaagactacc acatgaccag taatggtttc atagatagta tcataaatag
     5641 taaatgggtc ggactcgata attttagatt cttatttagt tcaagagacg cctttattat
     5701 cacacgaaat actgtcctct acaatcttgg ctttatcttt ctaggtttag ttgtatctgt
     5761 agggattgcc attatcctca gcgagctccg ttctaagaga atggtgaaga tttttcaaac
     5821 ttctatgttg ttcccttact tcttgtcttg ggttatcatc agtttcttta cagatgcctt
     5881 cctaaatatt gataaagggg tgttcaatca tctattggaa agtcttggtc tcaaagaagt
     5941 caatttctac gctgacctgg gcatctggcc ctatctccta cttttcctag gtatttggaa
     6001 aggctttgga tatagcagtg tcatgtacta tgcgacgatc atgggaattg atccaaccta
     6061 ctacgaagca gcgacagtgg acggagctag caagtggcaa cgtattcgca acgtaaccat
     6121 tcctcagttg actccgcttg taactgtatt gaccatcctt gcagtcggaa atatcttccg
     6181 cgcagacttc ggtctcttct atcaaatccc acacaatgct ggtcagcttt acaatgtaac
     6241 caacgttttg gatgtatatg tctttaatgg tttgactcag acagcagata tcggtatggc
     6301 tgcagcagcc ggtctttacc aatccgttgt tggtttgatt ctggttatcc tatcaaactt
     6361 gcttgcaaga cgagtcgatc caaactcagc tttgttctag aaaggaggag aatatggcaa
     6421 aaaagaaaat taaaaaagaa aaaattgata atgtcggcat tcactccttc agtaagaaag
     6481 cagatatctt cttcagtatc atttcgggtt tgattgccct ctcttgtatc ctaccttttg
     6541 tattcgttat cattatttca gtgacagacg agaaaagcct cctccaatac ggctatagct
     6601 tcttcccatc ccaatttggc ttagacggtt ttgagttttt ggcacagttt aaggataaaa
     6661 tcctacaagc cctcttcatc tctgtctttg taaccgtagt tgggacattg acaaatgtct
     6721 ttatcacaac aacctatgcc tacgctattt cacggacaac ctttaagtat cgcagatttt
     6781 ttaccatctt cgtccttctc agtatgttgt tcaacgctgg tttggtacca ggttatatca
     6841 tggtgacccg tgtacttcag cttggtgaca ctgtttgggc cttgattgtg ccaatgcttc
     6901 tctccccctt taacatcatc ttgatgcgtt ccttcttcaa gaagaccatt ccagaagcca
     6961 ttctcgaatc ggctcgtatc gatggtgcca gtgaggcccg gattttcttc caaatctgtt
     7021 tgccattgtc actaccaggt atcgcaacca tcacgctttt gacagctctt ggtttctgga
     7081 atgactggtt caacgccctt ctttacatca agagtgacaa cttgtatcca ttgcaatatt
     7141 tgctcatgca aattcaacaa aatatggact acattgccaa agcggtcggc ttgactggtc
     7201 aactgggagt tgctctaccg aaagaaacag gtcgtatggc catggttgtg gttgcaaccc
     7261 ttccaatcgc gattttatat ccattcttcc aacgctactt tgtaaaaggt ttgactatcg
     7321 gtggtgtgaa agaatagtgc ttattgaaaa agaacatttc tcagttccca acttcccttg
     7381 tgtgaagtta atactcttcg aaaatcaaat tcaaaccgcg tcaacgtcgc cttgccgtag
     7441 atatatgtaa ctgacttcgt cagtcttatc tacaacctca aagcagtgct ttgagcagcc
     7501 tgcggctagt tttctagttt gctctttgat tttcattgag tataagatca ttacattgtt
     7561 tataagttta aaaataaaaa aaggagtttt tatcatgaaa aactggaaaa aatatgcttt
     7621 tgcatctgct agtgtagtcg ctttggctgc aggtcttgct gcttgtggaa atttgacagg
     7681 taacagcaaa aaagctgctg attcaggtga caaacctgtt atcaaaatgt accaaatcgg
     7741 tgacaaacca gacaacttgg atgaattgtt agcaaatgcc aacaaaatca ttgaagaaaa
     7801 agttggtgcc aaattggata tccaatacct tggctggggt gactatggta agaaaatgtc
     7861 agttatcaca tcatctggtg aaaactatga tattgccttt gcagataact atattgtaaa
     7921 tgctcaaaaa ggtgcttacg ctgacttgac agaattgtac aaaaaagaag gtaaagacct
     7981 ttacaaagca cttgacccag cttacatcaa gggtaatact gtaaatggta agatttacgc
     8041 tgttccagtt gcagccaacg ttgcatcatc tcaaaacttt gccttcaacg gaactctcct
     8101 tgctaaatat ggtatcgata tttcaggtgt tacttcttac gaaactcttg agccagtctt
     8161 gaaacaaatt aaagaaaaag ctccagacgt agtaccattt gctattggta aagttttcat
     8221 cccatctgat aactttgact acccagtagc aaacggtctt ccattcgtta tcgaccttga
     8281 aggcgatact actaaagttg taaaccgtta cgaagtgcct cgtttcaaag aacacttgaa
     8341 gactcttcac aaattctatg aagctggcta cattccaaaa gacgtcgcaa caagcgatac
     8401 ttcctttgac cttcaacaag atacttggtt cgttcgtgaa gaaacagtag gaccagctga
     8461 ctacggtaac agcttgcttt cacgtgttgc caacaaagat atccaaatca aaccaattac
     8521 taacttcatc aagaaaaacc aaacaacaca agttgctaac tttgtcatct caaacaactc
     8581 taagaacaaa gaaaaatcaa tggaaatctt gaacctcttg aatacgaatc cagaactctt
     8641 gaacggtctt gtttacggtc cagaaggcaa gaactgggaa aaaattgaag gtaaagaaaa
     8701 ccgtgttcgc gttcttgatg gctacaaagg aaacactcac atgggtggat ggaacactgg
     8761 taacaactgg atcctttaca tcaacgaaaa cgttacagac caacaaatcg aaaattctaa
     8821 gaaagaattg gcagaagcta aagaatctcc agcgcttgga tttatcttca atactgacaa
     8881 tgtgaaatct gaaatctcag ctattgctaa cacaatgcaa caatttgata cagctatcaa
     8941 cactggtact gtagacccag ataaagctat tccagaattg atggaaaaat tgaaatctga
     9001 aggtgcctac gaaaaagtat tgaacgaaat gcaaaaacaa tacgatgaat tcttgaaaaa
     9061 caaaaaataa tcagctaaat tgatttcgtg tattcattcc taactcctaa aataagaatc
     9121 actgctttcc tcaggttggt ctggggaggg cagtggtttt tttggcacaa ggatatagat
     9181 atccatgttt tctcttgtcc ttattttaac cattcaggaa atttcgagaa tcctctacaa
     9241 tttccagtca aataaattgc attcgttttc tcaagcaggt atactagtat ggataaataa
     9301 aaaatttaga aaatttaaga atagaaaaga gaacaaatct tatggcaaaa gatattcgtg
     9361 tcttacttta ctacctttat actccaattg aaaatgcaga gcaatttgct gcagaccact
     9421 tggctttctg taaatcaatc ggccttaaag gccgtatcct agtcgctgac gagggaatta
     9481 acggaacagt ttcaggtgac tatgaaacaa ctcaaaaata catggactac gttcacagcc
     9541 ttccaggaat ggaagatctc tggttcaaga ttgacgaaga aagtgaacaa gccttcaaga
     9601 agatgtttgt tcgctacaag aaagaaattg tccaccttgg tttggaagac aacgactttg
     9661 acaatgacat caacccactt gaaacaacag gtgcttactt gtctccaaaa gagttcaaag
     9721 aagcgcttct tgataaagat accgttgtcc ttgacacacg taacgattat gagtacgacc
     9781 taggacattt ccgtggggct attcgcccag atattcgcaa cttccgtgag ttaccacaat
     9841 gggtccgtga caacaaagaa aaattcatgg acaagcgtgt cgtggtttac tgtacaggtg
     9901 gtgttcgctg tgagaaattc tcaggctgga tggttcgtga aggttacaaa gatgttggcc
     9961 aattgcacgg aggaatcgca acttacggta aagacccaga agttcaaggt gagctttggg
    10021 atgggaaaat gtacgtcttt gacgagcgta ttgccgttga tgtcaaccat gtcaacccaa
    10081 ccatcgtagg gaaagactgg tttgatggaa caccatgtga acgttatgtc aactgtggaa
    10141 atcccttctg taaccgtcgt atcttgacat cagaagaaaa tgaagacaag taccttcgtg
    10201 gatgctcaca cgagtgccgt gttcacccac gtaaccgcta tgtttcaaaa aatgaattga
    10261 cacaagctga agtgattgag cgcctagccg ctatcggtga aagcttggat caggcagcta
    10321 ctgtataaga tcaaacagtc cttaggggct gtttttctat gctttgtact caaaaatcta
    10381 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggctgct caaagcactg
    10441 ctttgaggtt gtagataaga ctgacgaagt cagttacata tatctacggc aaggcgaagc
    10501 tgacgcggtt tgaagagatt ttcgaagaat ataaagtttg tttttgtatc tttcagaaaa
    10561 ataaggtata ctgtatgtaa acgatttcaa aggagtccag ttatggcgaa aacatttttt
    10621 attccaaata aacagagcat tttaggagaa caagagattt tgaatgccaa gtcgatcttg
    10681 gccttgctag acggtttgga gtcacatagc tatgatgtag tctatctccg tcagcctctt
    10741 aatcgtctcg agtatattga gtgtgcgata gtggggcaat cacaatttct ctttaaggtc
    10801 agttatgctg atggtcaaaa ggcttaccgt gtcgatcttc ctgacctact aacaaagaca
    10861 gactggcaga ttatcaagtc atttttagat gctttgcttg cttatacagg gactgatatt
    10921 gaagggctag atggttttga ttttgaagct tatttccaag caagtattca agcctatcta
    10981 gcagaccctg tagctcgttt tacgatttgc caaggaattt ttaatcctat tttctttagt
    11041 cgtgagaact tgaaaagctt tttagaggca gatggcttgg ctcagtttga agcgcgtgtg
    11101 cgtgcggttc aagagacaga tgcctacttt gcgagagttt ccttctatca ggatggagaa
    11161 ggaaaagtgc atggcgttta ccatctagct caaggagtca agacagtttt accgagagaa
    11221 ccgtttgttc ctgcagccta tattgagcaa ttggtggata aggaagtcca gtgggagatt
    11281 gacttggttc aaatcacagg agacggctct aaaccagaag actatgaatc catagctcgc
    11341 ttggactatg caaaattctt agaggtatta cccccatctt tttaccacca actagacgcc
    11401 aatcaaatag aagtgcaacc catattagac aaagatttta aaacattagc acaagaaaag
    11461 taaagcagaa gcaggtcaat cgacttgctt ttttgaaata gaaaaatcct gccaaggatg
    11521 acaggattgc tactcaatga aaatcggctc tataatattt gtagtgggta aatcccctat
    11581 ggatattatg gagcctatta taacataaac gagtgacttt ttaagtaatg actgcgcttt
    11641 tcaatgatgg gagaggtatg gtattggttt taatcacaaa ttatcagaaa attttctatt
    11701 tttttgttga caatattttg aatatagtat acaataattt cattaactcg acaatcacta
    11761 gatatatgca atctaatcgt aattttatag gaggagtccg ctgatgtcaa acgagaatgc
    11821 acagaatttg atgtggagat aatttaggat ggatgaatca aaatatttgt gctatcttga
    11881 aaaatatgat gctttaggct caggacgatt attagctaaa gttaataata cttactatat
    11941 gtttaacaaa aatcagattg aattcttacg atactcaaat aaaaatgcta aaaaagagag
    12001 gaaatatatg ta
//
LOCUS       DAVHIT010000058        11987 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVHIT010000058 DAVHIT010000000
VERSION     DAVHIT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.059"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(133..1764)
                     /locus_tag="VVN16_001477"
     CDS             complement(133..1764)
                     /locus_tag="VVN16_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU5572831.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTAYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2057..7036
                     /locus_tag="VVN16_001478"
     CDS             2057..7036
                     /locus_tag="VVN16_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU5572832.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTVQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQMDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKTAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7126..8322
                     /locus_tag="VVN16_001479"
     CDS             7126..8322
                     /locus_tag="VVN16_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5572833.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8458..8985
                     /locus_tag="VVN16_001480"
     CDS             8458..8985
                     /locus_tag="VVN16_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU5572834.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9062..9418
                     /gene="glnR"
                     /locus_tag="VVN16_001481"
     CDS             9062..9418
                     /gene="glnR"
                     /locus_tag="VVN16_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU5572835.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9455..10801
                     /gene="glnA"
                     /locus_tag="VVN16_001482"
     CDS             9455..10801
                     /gene="glnA"
                     /locus_tag="VVN16_001482"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU5572836.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10897..11016)
                     /locus_tag="VVN16_001483"
     CDS             complement(10897..11016)
                     /locus_tag="VVN16_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU5572837.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11505..11987
                     /locus_tag="VVN16_001484"
                     /pseudo
     CDS             11505..11987
                     /locus_tag="VVN16_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3851 a         2179 c         2572 g         3385 t
ORIGIN      
        1 agaaatagag gtgtactatt ctagtttcaa tatactatat atctgcagaa tactctttca
       61 gtttcattca gcctagtctt gcttaggaaa aagtaaaaag ccatccaatt atgatggata
      121 gcttcttggt tcttagattt gttctgcaaa aaccttttca gcaaggttca tagcgtggtc
      181 tgatacacga gtgtagtgtg agatgatgtc gataaagttg accccagctt gtgttgaaca
      241 ttcgcctttg ttgaggcgtt tgatgtgggt ttttctgaga acacgttcta tcttattgat
      301 tgcttcatga cgttctacaa gactgcgtgc tttttcaata tcattgtttt ccacactatc
      361 cagagcatct ttgataaagt cactagtttg gcggtaaact tcctctaatt ctttcaaggc
      421 ggcatcagaa aattcaacat ttttccgttg aagataggca gtcagattga gtagagcctc
      481 cgtgtggtct ccaatccgtt ccaaatcacg ggaggaatca aggatattgg taagcacttc
      541 actttctttt tggctgagag cttcgcttga aagggcaatg agataacgtg ttaattgctc
      601 atcgatggtg ttaattgctt cttcggtttt atgccctttt tcagccactt tttcatccaa
      661 gtcaatgatg tacttatagg aaaggtcaaa ggctttagca gcgtagtttc ctaagtgcaa
      721 gagctctttt ttagcatttc ctagagcgat agatggggct tgtttgatga aatgttcatc
      781 aagatataag ggttcgtatt tgacaacctc gtcctctcca ggaataatct tggttacaaa
      841 gtaagccaga gctccgataa atggaaattg gacaatggtg ttggtaatat taaaggttcc
      901 gtgagcaaag gcgatggtca tttccggtgc tagatttagc gtagcttcaa accaatgaat
      961 caggacagta aaaggaacta gaaaaataac gcagacgact gttccgataa cgttgaaggc
     1021 aacatgagct cctgctaccc gtttagctgc aatattagcc cctaaagagg caatgatggc
     1081 tgtaatggtt gtcccgatat tgtcaccaaa tagaactggc aaagctccct gtaggtcaat
     1141 tagattgccg gcgtagaggt tttgtaaaat cccaatggta gccgaagaag cttgaattag
     1201 caaggtcaag ccagtaccga caaagacacc caaaacagga tttttactta gctcaatcat
     1261 atagtcctta aagacctgta aatccttgag tggagccatt gcgccgctca tgagattgag
     1321 ggcaaaaaag ataccaccga caccaaagag gatgcgtccg atattattga ctgtccgatt
     1381 tttcgtaaaa aagagacaga cggcaccgat aaagagcata ggtagggcat agttacctaa
     1441 tttaaaaccg atgagaaagg atgtgacagt tgtcccaata ttagcaccca tgacaatccc
     1501 gatagcctga cgtaaggtta agagaccggc actgaccagg ccgactgtaa taactgttac
     1561 accagaacta gactgaatta gagcagtcat cccaatacca accagaactc caaagaaagg
     1621 attactagta tatttgtcaa tgtaaaaacg aaggcgatct ccagcagctt gttgcaaacc
     1681 gtctcccatg gtcttgatgc tatataagaa tagccccaga ccacctaaaa agtgaaataa
     1741 aatttcctgc caattaatgg acatttcttt ttcctccgaa aaataatcgc ggaatatctc
     1801 ctattctatt ttaaaggata aaagtaaatc taacaagtgt taatgtcaag tttgtaaaag
     1861 aaaattataa ttgataagtg tattattttt aagagttttt aaccttattt tagatttgtg
     1921 caaacagtta ttgttttttg tctaaaaatg ttttaggata aaaatgtgat tatcatggtt
     1981 ctagattttg ataaaaaact acagaatata aagcgctttc tttaaaacaa caaaatcaat
     2041 cttttaggag gagaaaatga agaatccatt ttttgaaaga cgttgtcgtt acagtattcg
     2101 taagttatca gtaggagcct gctcgctgat gattggtgct gttttatttg ctggtccagc
     2161 cttggctgaa gaaactgcag ttcctgaaaa tagcggagct aatacagagc ttgtttcagg
     2221 agagagtgag cattcgacca atgaagctga taagcagaat gaaggggaac atgctagaga
     2281 aaacaagcta gaaaaggcag aaggagtagc gacagcatct gaaactgctt cgccagcaag
     2341 caatgaagct gcaactactg aaactgcaga agcagctagc gcagctaaac cagaggaaaa
     2401 agcaagtgag gtggttgcag aaacaccatc tgcagaagca aaacctaagt ctgacaagga
     2461 aacagaagca aagcccgaag caactaacca aggagatgag tctaagccag cagccgaagc
     2521 taataagact gaaaaagaag tccagccaga tgtccctaaa aatacagaaa aaacattaaa
     2581 accaaaggaa atcaaattta attcttggga agaattgtta aaatgggaac caggtgctcg
     2641 tgaagatgat gctattaacc gcggatctgt tgtcctcgct tcacgtcgga caggtcattt
     2701 agtcaatgaa aaagctagca aggaagcaaa agttcaagcc ttatcaaaca ccaattctaa
     2761 agcaaaagac catgcttctg ttggtggaga agagttcaag gcctatgctt ttgactattg
     2821 gcaatatcta gattcaatgg tcttctggga aggtctcgta ccaactcctg acgttattga
     2881 tgcaggtcac cgtaacgggg ttcctgtata cggtacactc ttcttcaact ggtctaatag
     2941 tattgcagat caagaaagat ttgctgaagc tttgaagcaa gacgcagatg gtagctttcc
     3001 aattgcccgt aaattggtag acatggcaaa gtattatggc tatgatggct atttcatcaa
     3061 ccaagaaaca actggagatt tggttaaacc tcttggagaa aagatgcgcc agtttatgct
     3121 ctatagcaag gaatatgctg ctaaggtaaa ccatccaatc aagtattctt ggtatgatgc
     3181 catgacctat aactatggac gttaccatca agatggtttg ggagaataca actaccaatt
     3241 catgcaacca gaaggagata aggttccggc agataacttc tttgctaact ttaactggga
     3301 taaggctaaa aatgattaca ctattgcaac tgccaactgg attggtcgta atccttatga
     3361 tgtatttgca ggtttggaat tgcaacaggg tggttcctac aagacaaagg ttaagtggaa
     3421 tgacatttta gacgaaaatg ggaaattgcg cctttctctt ggtttatttg ccccagatac
     3481 cattacaagt ttaggaaaaa ctggtgaaga ttatcataaa aatgaagata tcttctttac
     3541 aggttatcaa ggagacccta ctggccaaaa accaggtgac aaagattggt atggtattgc
     3601 taacctagtt gcggaccgta cgccagcggt aggtaatact tttactactt cttttaatac
     3661 aggtcatggt aaaaaatggt tcgtagatgg taaggtttct aaggattctg agtggaatta
     3721 tcgttcagta tcaggtgttc ttccaacatg gcgctggtgg cagacttcaa caggggaaaa
     3781 acttcgtgca gaatatgatt ttacagatgc ctataatggc ggaaattccc ttaaattctc
     3841 tggtgatgta gccggtaaga cagttcagga tgtgagactt tattctacta agttagaagt
     3901 aactgagaag accaaacttc gtgttgccca caagggagga aaaggttcta aagtttatat
     3961 ggcattctct acaacttcag actacaaatt cgatgatgca gatgcatgga aagagctaac
     4021 cctttctgac aactggacaa atgaagaatt tgatctcagc tcactagcag gtaaaaccat
     4081 ctatgcagtc aaactatttt tcgagcatga aggtgctgta aaagattatc agttcaacct
     4141 aggacaatta actatctcgg acaatcacca agagccacaa tcgccgacaa gcttttctgt
     4201 agtgaaacaa tctcttaaaa atgcccaaga agcggaagca gttgtgcaat ttaaaggcaa
     4261 caaggatgca gatttctatg aagtttatga aaaagatgga gacagctgga aattactaac
     4321 tggctcatct tctacaacta tttatctacc aaaagttagc cgctcagcaa gtgctcaggg
     4381 tacaactcaa gaactgaagg ttgtagcagt cggtaaaaat ggagttcgtt cagaagctgc
     4441 aaccacaacc tttgattggg gtatgactgt aaaagatacc agcctaccaa aaccactagc
     4501 tgaaaatatc gttccaggtg caacagttat tgatagtact ttccctaaga ctgaaggtgg
     4561 agaaggtatt gaaggtatgt tgaacggtac cattactagc ttgtcagata aatggtcttc
     4621 agctcagttg agtggtagtg tggatattcg tttgaccaag ccacgtaccg ttgttagatg
     4681 ggtcatggat catgcaggag ctggtggtga gtctgttaac gatggcttga tgaacactaa
     4741 agactttgac ctttattata aagatgcaga tggtgagtgg aagctagcta aggaagtccg
     4801 tggtaacaaa gcacacgtga cagatatcac tcttgataaa ccaatcactg ctcaagactg
     4861 gcgcttgaat gttgtcactt ctgacaatgg aactccatgg aaggctattc gtatctataa
     4921 ctggaaaatg tatgaaaagc ttgatactga gagtgtcaat attccgatgg ccaaggctgc
     4981 agcccgttct ctaggcaata acaaggtaca agttggcttt gcagatgtac cggctggagc
     5041 aactattact gtttatgata atccaaattc tcaaactccg cttgcaacct tgaagagcga
     5101 agttggagga gacctagcaa gtgcaccatt ggatttgaca aatcaatctg gtcttcttta
     5161 ttatcgtacc cagttgccag gcaaggaaat tagtaatgtc ctagcagttt ccgttccaaa
     5221 agatgacaga agaatcaagt cagtcagcct agaaacagga cctaagaaaa caagctacgc
     5281 cgaaggggag gatttggacc ttagaggtgg tgttcttcga gttcagtatg aaggaggaac
     5341 tgaggacgaa ctcattcgcc taactcacgc aggtgtatca gtatcaggtt ttgatacgca
     5401 tcataaggga gaacagaatc ttactctcca atatttggga caaccggtaa atgctaattt
     5461 gtcagtgact gtcactggcc aagacgaagc aagtccgaaa actattttgg gaattgaagt
     5521 aagtcaggaa ccgaaaaaag attacctagt tggtgatagc ttagacttgt ctgaaggacg
     5581 ctttgcagtg gcttatagca atgacaccat ggaagaacat tcctttactg atgagggagt
     5641 tgaaatttct ggttacgatg ctcaaaagac tggtcgtcaa accttgacgc ttcattacca
     5701 aggccatgaa gttagctttg atgttttggt atctccaaaa gcagcattga acgatgagta
     5761 cctcaaacaa aaattagcag aagttgaagc tgctaagaac aaggtggtct ataactttgc
     5821 ttcatcagaa gtaaaagaag ccttcttgaa agcaattgaa gcggccgaac aagtgttgaa
     5881 agaccatgaa actagcaccc aagatcaagt caatgaccga cttaataaat tgacagaagc
     5941 tcataaagct ctgaatggtc aagagaaatt taaggaagaa aagacagagc ttgatcgctt
     6001 aacaggtgag gttcaagaac tcttggctgc caaaccaaac catccttcag gttctgccct
     6061 agctccgctt cttgagaaaa acaaggcctt ggttgaaaaa gtagatttga gtccagaaga
     6121 gcttacaaca gcgaaacaga gtctaaaaga tctggttgct ttattgaaag aagacaagcc
     6181 agcagtcttt tctgatagta aaacaggtgt tgaagtacac ttctcaaata aagagaagac
     6241 tgtcatcaag ggtttgaaag tagagcgtgt tcaagcaagt gctgaagaga agaaatactt
     6301 tgctggagaa gatgctcatg tctttgaaat agaaggtttg gatgaaaaag gtcaagatgt
     6361 tgatctctct tatgcttcta ttgtgaaaat cccaattgaa aaagataaga aagttaagaa
     6421 agtatttttc ttacctgaag gcaaagaggc agtagaattg gcttttgaac aaatggatag
     6481 tcatgttatc tttacagcac ctcactttac tcattatgcc tttgtttatg aatctgctga
     6541 aaaaccacaa cctgctaaac cagcaccaca aaacacagtc cttccaaaac ctacttatca
     6601 accggcttct gatcaacaaa aggctcctaa attggaagtt caagaggaaa aggttgcctt
     6661 tcatcgtcaa gagcatgaaa atgctgagat gctagttggg gaacaacgag tcatcataca
     6721 gggacgagat ggactgttaa gacatgtctt tgaagttgat gaaaacggtc agcgtcgtct
     6781 tcgttcaaca gaagtcatcc aagaagcgat tccagaaatt gttgaaattg gaacaaaagt
     6841 aaaaacagta ccagcagtag tagctacaca ggaaaaaaca gctcaaaata cagcagttaa
     6901 atcagaagaa gcaagcaaac aattgccaaa tacaggaaca gctgatgcta atgaagccct
     6961 aatagcaggc ttggccagcc ttggtcttgc tagtttagcc ttgaccttga gacggaaaag
     7021 agaagataaa gattaaatat cgaaaaatct tgtgaaatct ttccttatat ttccaaagtg
     7081 tgatataata gtttcgaata aaataaataa aggagttttt gtaacatggc aaaacttact
     7141 gttaaagacg ttgacttgaa aggtaaaaaa gtcctcgttc gtgttgactt caacgtacca
     7201 ttgaaagatg gcgtaatcac taacgataac cgtatcacag cagctcttcc aactattaag
     7261 tacatcatcg aacaaggtgg acgtgcaatt cttttctctc accttggacg tgtgaaagaa
     7321 gaagctgata aagctggcaa atcacttgct cctgtagcag ctgacttggc agcaaaactt
     7381 ggtcaagatg ttgttttccc aggtgtcact cgtggtgctg aattggaagc ggcaatcaac
     7441 gctcttgaag atggacaagt tctcttggtt gaaaacactc gttacgaaga tgttgacggc
     7501 aagaaagaat ctaaaaacga tcctgaactt ggtaaatact gggcatcact tggagatggt
     7561 atcttcgtaa acgatgcatt cggtacagct caccgtgcac acgcatctaa cgttggtatc
     7621 tcagcaaacg ttgaaaaagc agttgctggt ttccttcttg aaaacgaaat tgcctacatc
     7681 caagaagcag ttgaaactcc agaacgtcca ttcgtggcta tccttggtgg ttcaaaagtt
     7741 tcagacaaga tcggtgttat cgaaaacttg cttgaaaaag ctgataaagt ccttatcggt
     7801 ggtgggatga cttacacatt ctacaaagca caaggtatcg aaatcggtaa ctcacttgta
     7861 gaagaagaca aattggatgt tgcgaaagct cttcttgaaa aagcaaatgg taaattgatc
     7921 ttgccagttg actcaaaaga agctaacgca tttgctggtt acactgaagt gcgtgacact
     7981 gaaggtgaag cagtttctga aggcttcctt ggtcttgaca tcggtccaaa atctatcgcc
     8041 aaatttgacg aagctttgac tggtgccaaa acagttgtat ggaacggacc tatgggtgta
     8101 tttgaaaacc cagatttcca agctggtaca atcggtgtga tggacgctat cgtgaaacaa
     8161 ccaggagtta aatcaatcat cggtggtggt gactcagctg ccgcagcgat taaccttggc
     8221 cgtgcagaca agttctcatg gattagtacg ggtggtggag catcaatgga acttcttgaa
     8281 ggtaaggttc ttccaggact tgcagccttg acagaaaaat aagattttat aaacaaatca
     8341 aagaagagag ggatgaaagt tcctcttttc ttttgcttaa aataaaaacg cttcctctca
     8401 actattactc ataaaaatca ccgatttatg ataaaatgga aatagaaagt tgagattatg
     8461 agttatttta aaaaatataa attcgataaa tcccagttca aacttggtat gcgaaccttt
     8521 aaaacaggta ttgctgtttt tctagttctc ttgatttttg gcttttttgg ctggaaaggt
     8581 cttcaaattg gtgctttgac agccgttttt agcctgaggg agagttttga tgagagtgtt
     8641 cattttggga cttcgcgtat tctaggaaat agtatcggtg gactctatgc cttggtcttc
     8701 ttcttattaa atactttttt ccacgaagcc ttttgggtga ccttggtagt tgttccaatc
     8761 tgcaccatgt taaccattat gacaaatgta gccatgaata acaaagcagg ggttattggt
     8821 ggtgtagcag ctatgttaat cattacccta tcaattccaa gtggtgagac aattttgtac
     8881 gtgtttgtgc gtgtattaga aacgtttatg ggagtttttg tcgcaattat cgtaaattac
     8941 gatattgatc gtattcgtct ctttttagag aaaaaagaaa aataatgtta cattttataa
     9001 cattatcaat tgacgtttgt ctttttttag actataatag acagaaagaa ggaaattgta
     9061 aatgaaggaa aaagaatttc gccgaaatat ggctgttttt cctatcggca gtgttatgaa
     9121 gttgaccgat ttatcggcgc gtcagattcg ttattatgaa gatcaagagt tgatcaagcc
     9181 cgatcgaaac gaaggaaatc gtcgcatgta ttccttgaat gacatggatc gtctgcttga
     9241 aatcaaagat tatatctctg aaggttataa tatcgctgcc attaagaaaa aatatgctga
     9301 acgtgaagcg aaatccaaga aagcggttag tcagactgaa gtacgtcgtg cacttcacaa
     9361 tgaactcctc caacaggggc gttttgcttc agtacagtca ccttttggtc gcggttaggc
     9421 aaccgcaagt agtcatagat taaggagaaa acttatgcca atcacagctg cagatattcg
     9481 tcgtgaagtc aaggaaaaaa atgttacctt tattcgtctt atgttctcag atattttggg
     9541 aaccatgaaa aacgtcgaaa ttcctgctac agatgaacag ttagataagg tcttgtcgaa
     9601 caaggttatg tttgatggat cttctattga aggttttgta cgtatcaatg agtcggatat
     9661 gtacttgtac ccggacttgg atacatggac agtcttccct tggggagatg aaaatggaag
     9721 tgttgcaggt ctgatctgtg atgtttatac aacagaaggt gaaccatttg cgggtgaccc
     9781 tcgtggtaat ttgaaacgag ctcttcgtca catggaagaa gttggattca aatccttcaa
     9841 ccttggtcca gagccagaat tcttcctatt taagttggat gaaaatgggg acccaacact
     9901 tgaagtgaat gacaagggtg gctactttga tttggcacct actgaccttg cggacaacac
     9961 acgtcgtgag attgtgaatg tcttgaccaa aatgggattt gaagtagaag cgagtcacca
    10021 cgaggttgcg gttggacagc atgagattga ctttaagtac gatgaagttc tccgtgcttg
    10081 tgataagatt caaatcttta aacttgttgt taaaaccatt gctcgcaaac acggacttta
    10141 cgcaacattt atggcgaagc caaaatttgg tattgctggc tcaggtatgc actgtaatat
    10201 gtccttgttt gatgcagaag gaaataacgc cttctttgat ccaaatgatc caaaaggaat
    10261 gcagttgtca gaaacagctt accatttcct aggcggtttg atcaagcatg cttacaacta
    10321 tactgccatc atgaacccaa cagttaactc atacaaacgt ttggttccag gttatgaagc
    10381 gcctgtttac attgcttggg ctggtcgtaa ccgttcgcca cttgtgcgcg tacctgcttc
    10441 acgtggtatg ggaactcgtc ttgagttgcg ttcagtggat ccaatggcga acccttacgt
    10501 tgctatggct gttcttttgg aagttggttt gtatggtatt gaaaataaaa tcgaagcacc
    10561 agctcctatc gaagaaaata tctacatcat gacagcagaa gagcgcaagg aagctggtat
    10621 tacagacctt ccatcaactc ttcacaacgc tttgaaagct ttgacagaag atgaagtggt
    10681 taaagctgct ctcggagatc acatctacac tagcttcctt gaagccaaac gaatcgaatg
    10741 ggcaagttat gcaaccttcg tttcacaatg ggaaattgat aattatttag acctttacta
    10801 atactaatat agaagaaaga ttgcctgtgg gtaaacagtc acacagcagt ctaagccatc
    10861 aaatacctat aacccttaaa atagcgattt cggtcgctat ttatttttcg aaaaatcctc
    10921 ttgactcttt tgcgaattag atttatcatc agacgtgtag aagaaaatca aggagtcact
    10981 atggaaaaat tcaatttcaa aaacaatatc gggcaagaaa acaagttact tcaaatcgaa
    11041 atctacaagt ttactaactt ttacaagctt caaaattaca catctgtaaa tattttttcg
    11101 aaagatatat ttgaagccat agtaaattga gactagaata gtacactaca tctgctaaaa
    11161 atttctagaa actaatttaa ctttcctaat cgatttgttt acatcttatt tcaatctact
    11221 atattatctc aataaatgat gatggcgact gttttgtttg tgcttgtcga tttttgaatc
    11281 cctgatcgag cggaagaata tttctgccga ggttgccaag gggaaaatac tgaagatatt
    11341 gaattttatg atgaacaatt acaggcagaa aaggtggagg ttttgaatat tcgattggaa
    11401 gtaaaaaatt gaattatcta caatctcatc aacaataaac ctatttcaac cccattatct
    11461 ataggcgtat ttttacgcct ttttatatac ttgaagcaca taagaaacat tatgggaagt
    11521 ttgctgatgg aagcactcaa gaaattgatg ttccttatga tattcctgat acttgggagt
    11581 gggtgaggat aaaatcaatt tattggaatt ttgggcaaaa taagccagag aaatccttta
    11641 ggtatataga tacgtctagt attgatagaa aaaagaacat aatcaactac aaaaatctac
    11701 aatatctttc acctgaacaa gcgccttccc gtgctagaaa attagtttcg cagaatagtg
    11761 tcttattttc aacagttaga ccatatctaa aaaatattgc tgtagttaga gaacttaaag
    11821 agtatttgat agctagtaca gcatttattg ttttggatac tttacttaac gaaacatatt
    11881 tgaaatatta tttattatca gataatttta tcaatcgtgt taataataaa tctactggaa
    11941 caagttatcc tgcaatcaat gattataatt ttaatctatt attaatt
//
LOCUS       DAVHIT010000059        11665 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVHIT010000059 DAVHIT010000000
VERSION     DAVHIT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.060"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1016
                     /locus_tag="VVN16_001485"
     CDS             <1..1016
                     /locus_tag="VVN16_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5572838.1"
                     /translation="RDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGY
                     SDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQ
                     EQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPH
                     RVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLS
                     KDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKS
                     DSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKK
                     NQD"
     gene            1648..2124
                     /locus_tag="VVN16_001486"
     CDS             1648..2124
                     /locus_tag="VVN16_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5572839.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPGK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            2162..2464
                     /locus_tag="VVN16_001487"
     CDS             2162..2464
                     /locus_tag="VVN16_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5572840.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAKQNMV"
     gene            2472..3275
                     /locus_tag="VVN16_001488"
                     /pseudo
     CDS             2472..3275
                     /locus_tag="VVN16_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            3312..3788
                     /locus_tag="VVN16_001489"
     CDS             3312..3788
                     /locus_tag="VVN16_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU5572841.1"
                     /translation="MPYFIGGHPGFNCPLLDDGVYEDYYLESEKEETCSVPRPFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDKGLKVHFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGEHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            3945..4370
                     /gene="lacA"
                     /locus_tag="VVN16_001490"
     CDS             3945..4370
                     /gene="lacA"
                     /locus_tag="VVN16_001490"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU5572842.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFIVATKIKGMVAAEVSDERSAYMTRSHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            4401..4916
                     /gene="lacB"
                     /locus_tag="VVN16_001491"
     CDS             4401..4916
                     /gene="lacB"
                     /locus_tag="VVN16_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU5572843.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHVETHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            4927..5856
                     /locus_tag="VVN16_001492"
     CDS             4927..5856
                     /locus_tag="VVN16_001492"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU5572844.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGNLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            5858..6838
                     /gene="lacD"
                     /locus_tag="VVN16_001493"
     CDS             5858..6838
                     /gene="lacD"
                     /locus_tag="VVN16_001493"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU5572845.1"
                     /translation="MALTEQKRARLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            7072..7908
                     /locus_tag="VVN16_001494"
     CDS             7072..7908
                     /locus_tag="VVN16_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU5572846.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            7944..8261
                     /locus_tag="VVN16_001495"
     CDS             7944..8261
                     /locus_tag="VVN16_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5572847.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            8261..9952
                     /locus_tag="VVN16_001496"
     CDS             8261..9952
                     /locus_tag="VVN16_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5572848.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALVFVQEQFD"
     gene            10167..11573
                     /gene="lacG"
                     /locus_tag="VVN16_001497"
     CDS             10167..11573
                     /gene="lacG"
                     /locus_tag="VVN16_001497"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU5572849.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLNL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLIDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         3831 a         2014 c         2380 g         3440 t
ORIGIN      
        1 ctagagattt taatattaag ctgaatgtta aagacggtga catcatggac tggggaatga
       61 aagactataa agcaaacgga tttccagata aggtaacaga tatggatgga aatgtttatc
      121 ttcaaactgg ctatagcgat ttgaatgcta aagcagttgg agtccactat cagtttttat
      181 atgataatgt taaacccgaa gtaaacattg atcctaaggg aaatactagt atcgaatatg
      241 ctgatggaaa atctgtagtc tttaacatca atgataaaag aaataatgga ttcgatggtg
      301 agattcaaga acaacatatt tatgtaaatg gaaaagaata tacatcattt gatgatatta
      361 aacaaataac agacaagaca ctaaacatta agattgttgt aaaagatttt gcaagaaata
      421 caaccgtaaa agaattcatt ttaaataaag atacgggaga ggtaagtgaa ttaaaacctc
      481 atagggtaac tgtgaccatt caaaatggaa aagaaatgag ttcaacgata gtgtcggaag
      541 aagattttat tttacctgtt tataagggtg aattagaaaa aggataccaa tttgatggtt
      601 gggaaatttc tggtttcgaa ggtaaaaaag acgctggcta tgttattaat ctatcaaaag
      661 atacctttat aaaacctgta ttcaagaaaa tagaggagaa aaaggaggaa gaaaataaac
      721 ctacttttga tgtatcgaaa aagaaagata acccacaagt aaaccatagt caattaaatg
      781 aaagtcacag aaaagaggat ttacaaagag aagatcattc acaaaaatct gattcaacta
      841 aggatgttac agctacagtt cttgataaaa acaatatcag tagtaaatca actactaaca
      901 atcctaataa gttgccaaaa actggaacag caagcggagc ccagacacta ttagctgccg
      961 gaataatgtt tatagtagga atttttcttg gattgaagaa aaaaaatcaa gattaagaca
     1021 aaagctatag aaaaaaatgg tttatgtact gagattagat agtgaggtga tgacatagtt
     1081 ttgtgaaaac ggccatttat aactcaatta tttagtttac ttcacttcac tattgatact
     1141 atttggagtt attaatggac ttagtttaga taactaatga attgattgaa agggttagta
     1201 ttgacaatat tggtcatatt gattagaaaa tagagtctat caaaatttaa aggctaatag
     1261 aggtgatgag acaatttcaa ggaattaagt agcgtttggt tgcttaattc ctttttattt
     1321 ggaaggaagt ttttcgtaac cgcccaataa cgaagtctat tggacggtta cgatatgatc
     1381 actgacttcg tcagtcttat ctacaacctc aaaactgtgt tttgagcaac ctgcggctag
     1441 cttcctagtt tactctttga ttttcattga atattagaac agcaaaaatg cttggagtat
     1501 ttgtttgtgt gtttattttt atataacaaa ctataaacaa aacaaaaata taaaaaagag
     1561 acaaaaaaag aacagaaagt aattaacaac gatttcatat agtgttatac ttatggcata
     1621 aagttaatgg aaagaaggga aaaccttatg gtattagatt atttctttga caaaaacctt
     1681 gtgttttgct tagaagcgga taatcaagaa caactctttg atcaggtagc aagcttgttg
     1741 gaagaacgag aaattgtcac tcctacttat cgtgaagcct tgatcacgcg tgaaaagtca
     1801 tttccaactg gtttagatat ggaatttcta ggaaaggact tgccaaatgt agcgattcct
     1861 cacacagata ttgttcacaa tctggctgaa aaagttgtgg tcgttcgatt agagaaaccg
     1921 gtgactttcc ataatatgat tgcgccaggt aaggaagtag aagtatcctt gctcttcttt
     1981 atcatcaata attcaagttc aagtcaaacg aacattcttg ctcagttgat ggacttcttc
     2041 acaggaaatg gtcatcttga agacctatca aaaatttctg aaccagaaaa actttatgct
     2101 tacattgctg aagcaaccgc ttaatcttgt ctattaaaaa ataaaatcgg aggaaatcca
     2161 aatgattaaa attcttgctg cctgcggtgc aggtgttaac tcaagtcacc aaattaaaag
     2221 tgctctagaa gaagaacttt caaaccgtgg ttatgatgtt cactgtgatg cagtcatggt
     2281 gaaagatgta aacgaagacc ttatgaaagg ttacgatatc tttacaccaa tcgctgcaac
     2341 agaccttggt tttgaaccag gtatcccagt tatcgaagct gggccaatct tattctgtat
     2401 cccagcaatg agcgttccag tatttgacaa tattagactt cctgcgaaac aaaatatggt
     2461 atagtagttc tatgaatgat gaagcaagta aacaactaac tgatgcacga tttaagcgtc
     2521 ttgttggtgt tcagcgtacc acttttgaag agatgttagc tgtattaaaa acagcttatc
     2581 aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac cttcttatgg
     2641 ccactcttca atagtgcgag aatatcgaac ttatgaacaa attgcggctg attttggtat
     2701 tcacgaaagc aactttatcc gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg
     2761 tgttacggtt tcaagaactc ctctcagctc tgaggacacg gtaatgattg atacgacgga
     2821 agtacaaatc aatcgcccta aaaaaagaat tagcgaatta ttctggtaaa aagaaatgcc
     2881 acgctatgaa ggctcaagcg attgtcacaa gtcaagggag aattgtttct ttggatatca
     2941 ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag ttgcagaaat atcggacaag
     3001 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagtac
     3061 aaactccacg taaatccagc aaactcaagt cgctaatagc tgaagataaa gcctataacc
     3121 atgcgctatc taaggagaga agcaaggttg agaacatctt tgccaaagta aaaacgttta
     3181 aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga
     3241 gtgctggtat tatcaatcat gaactaggat tctagttttg caggaagtct aataaagaaa
     3301 ctagcaaggt catgccttac tttattggtg gacatccagg atttaattgt cctctacttg
     3361 atgatggagt ctatgaagat tattatctag agtctgagaa agaagagact tgctctgttc
     3421 cacgtccttt cccagaaaca ggtatgttgg atttccaaga tagaagtcca tggctagagg
     3481 gacaaaaaga aatagatctt agttatgatc ttttcagcaa agatgcagtg actttggatg
     3541 aattgcaatc tcgaacaatt gcccttcgtt ctcttaaaca tgataaggga ttgaaagtgc
     3601 attttgcaga atttccaaac ctcatcatct ggtcaacttt gaacaaggga cctttcatta
     3661 cctttgaacc atggtctggc ttgtcaacat tccttgaaga aggagaacat ttagaagata
     3721 agaagaatgt ttgtctctta gaagccaatc aggttgaaga attagggttt gagatagaag
     3781 ttttataaaa aagtaaaaaa taaataacaa aaacaaacac aaactattga cttcttcaaa
     3841 caatagtagt atattatgtt tgtgaggact aattgactgt tttttaaaga cagccaatac
     3901 actgctggaa tccagcatag aaaaaataaa aaggagtata caatatgtct attgttatcg
     3961 gtgcagatgc tgcaggtttg agattgaaag aagttgtgaa agacttctta gaaaaagaaa
     4021 acttccacct tgtggatgtt acagctgaag gtcaagactt tgttgatgtg actctcgctg
     4081 ttgctgcaga agtaaacaaa gaagaacaaa accttggtat cgtgattgat gcttatggag
     4141 ctggtccatt tatagttgca actaagatca aaggaatggt tgctgcagaa gtatctgacg
     4201 aacgttcagc ttatatgact cgtagccaca acaactcacg tatgatcact atgggggcac
     4261 aacttgttgg tgatgaattg gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg
     4321 acggtggacg tcatcaaatc cgggttgaca tgttgaacaa aatgggctaa ttagattaca
     4381 gtaagaaagg taagttaaaa atgagaattg caattggatg cgaccacatc gtaacagatg
     4441 aaaaaatggc ggtttcagaa tttttgaaat caaaaggata tgaagtcatt gactttggta
     4501 catatgacca tacacggact cactacccaa tctttggtaa aaaagtaggg gaagctgtaa
     4561 ctagcggcca agctgatctt ggagtatgta tctgtggtac tggtgttggt atcaacaacg
     4621 ctgtaaataa agttccaggt gttcgctctg ccttggttcg tgatatgaca acagcccttt
     4681 atgctaaaga acaattgaac gccaacgtta tcggttttgg tggtaagatt actggtgaat
     4741 tgctcatgtg cgatatcatc gaagctttca tccatgctga atacaaacca tctgaagaaa
     4801 acaaaaaatt gattgcgaaa attgaacatg ttgaaacaca caatgctcaa caaacagatg
     4861 caaacttctt cactgaattc cttgaaaaat gggatcgcgg agaataccac gactaagagg
     4921 tgacctatga ttttaacagt cacaatgaac ccatccattg atatttccta tcccttggat
     4981 gagttgaaga ttgatactgt caatcgtgtg gtagatgtaa ccaaaacggc tggtggtaaa
     5041 gggctcaatg ttacacgcgt actttcagaa tttggcgatt ctgttcttgc tactggttta
     5101 gtaggtggca accttggtga gtttttggtt gaacatatcg ataatcaagt aaagaaagat
     5161 ttcttctcaa ttcagggaga aactcgtaac tgtatcgcta ttctccacgg agacaaccaa
     5221 acagaagttc ttgaaaaagg tcctgaagta ttggaacagg aaggtcaaga ctttttggaa
     5281 catttcaaaa aactcttgga gtcagttgaa gtagtggcca tctcaggtag tctgccagct
     5341 ggccttccag ttgattacta tgcgagcttg gtagaacttg ctaatcaagc tggcaagcct
     5401 gtagtcttgg actgctcagg tgcagcactt caggctgttc ttgaatcacc ccataaacca
     5461 acagtcatca aaccaaataa tgaagaattg tctcagcttc ttggaagaga agtttctgag
     5521 gatttggatg aattaaaaga agtacttcaa gaacctttgt ttgcagggat tgaatggatt
     5581 atcgtttcac ttggtgccaa cggtactttt gccaaacatg gtgacacttt ctacaaggta
     5641 gatattccta gaattcaggt ggtaaatcct gttggatctg gagactctac tgtggcagga
     5701 atttcttcag gacttcttca caaagaatcg gatgcagaat tactcatcaa ggcaaatgtc
     5761 cttggtatgc tcaatgctca agaaaaaatg actggtcatg tcaacatggc caactatcaa
     5821 gttctatatg atcaattaat agtaaaagag gtataaaatg gctttaacag aacaaaaacg
     5881 tgcacgctta gaaaaacttt ctgatgaaaa tggtatcatc tcagctcttg catttgacca
     5941 acgtggtgct ttgaaacgcc tcatggctca acaccaaaca gaagaaccaa ctgtggctca
     6001 aatggaagaa ctgaaagtct tggtagcaga tgaattgact aaatacgctt catcaatgct
     6061 tcttgaccct gagtatggac ttccagcaac taaagctctt gatgaaaaag ctggtcttct
     6121 ccttgcttat gaaaaaacag gttatgacac aacaagtaca aaacgcttgc cagactgctt
     6181 ggatgtttgg tctgcaaaac gtattaaaga agagggtgca gatgcagtta aattcttgct
     6241 ttactatgat gtagatagtt cagacgaact caaccaagaa aaacaagctt atatcgagcg
     6301 tatcggttct gagtgtgtgg ctgaagatat cccattcttc cttgaaatcc ttgcttacga
     6361 tgaaaaaatt gcagacgcag gttctgtaga atatgcgaaa gtaaaaccac acaaagttat
     6421 cggtgctatg aaagtctttt cagacccacg ctttaacatt gatgtcttga aagttgaagt
     6481 tcctgttaac attaaatatg ttgaaggctt cgctgaaggt gaagtggttt acacacgtga
     6541 agaagcagca gccttcttca aagcgcaaga tgaagcaacg aacttgccat acatttactt
     6601 gagtgctggt gtatcagcta aactcttcca agatactctt gtatttgctc atgaatcagg
     6661 tgcaaacttt aacggagttc tttgtggccg tgctacatgg gcaggatcag ttgaagctta
     6721 catcaaagat ggtgaagcag cagctcgcga atggcttcgc acaactggat ttgaaaacat
     6781 tgatgagctc aataaagttc ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga
     6841 aagtcctcct agtttaggaa catgaatcta aaaaaattta aaaaaagttg tatgtaaagg
     6901 tttacaaaat aacttacttg tgctatactt aaatcacaag ttaatacaag gtgagtgtta
     6961 ctaagtaata ttaggcatga tcacaggtga attagaaatc agctgatttt ctagttcatt
     7021 tgtggtcatt ttttgtactc atatactttt aagatataaa aggaggttga catgtatcga
     7081 attctaaatc caatgaatca caatgtctcg cttgtcagaa atgataaggg agaagaggtg
     7141 attgtaattg gtaaggggat tgcattcgga aagaagaagg gggatttgat tgctgaaaat
     7201 caggttgaga aaatctttcg gatgaagacc gaagagtcca gagaaaactt tatggctctt
     7261 ctcaaagatg ttccgcttga ttttatcaca gtgacctatg aaatcattga taagctatca
     7321 aagaaatatc attatccgat tcaagagtat ctctatgtaa ccttgacaga tcatatttac
     7381 tgttcttatc aagctctaac tcaaggaagg tacaaggata gtaatctgcc agatatttcc
     7441 gctaagtatc ctgtcgcttt tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag
     7501 ctagcagatc attttcctga ggacgaaatt attcggattg cttatcattt cattaatgct
     7561 gaaggtgaaa atgaagtgga acttgtggag tcgattgata agaggaaaga aattctcagg
     7621 aatgttgaag aagttttaac ggactatgca attcaacgaa ctaaaaagaa taaccatttc
     7681 tatgatcgct ttatgatcca tttgaattat ttcttggatt atttagacag atctagagat
     7741 gataaccaat cacttctgga tatggaagat catattaaac aatcctatcc aaaagccttc
     7801 gagattggtt ccaagatcta tgatgtgatt acgcaacata cgggtcttga tttgtataaa
     7861 agtgaacgag tttatctagt tctacatatc caacgtttat tgtcataaaa atttatttaa
     7921 aactatataa ggagaattct atcatgaata gagaagaagt aacattgtta ggttttgaaa
     7981 tcgtagccta tgctggcgat gctcgttcaa aactattgga agccttgaag gctgctgaag
     8041 ctggtgattt tgaaaaagcg gacgctctgg tagaggaagc tggtagctgt attgcagagg
     8101 ctcaccacgc gcaaacaagt ctattgacta aggaagcttc aggtgaggac ttggcttata
     8161 gtgtaaccat gatgcatggc caagaccact taatgacaac tatcttgtta aaagatttga
     8221 tgcatcattt aattgaactc tacaagagag gagttaaata atgaataaac taattgcatt
     8281 tatcgagaaa ggaaagcctt tctttgaaaa actatctcgt aatatctatc ttcgtgctat
     8341 tcgtgatggt ttcattgcag gtatgcctgt tattctcttc tcaagtatct ttatcttgat
     8401 tgcctttgta ccaaactcat ggggctttaa atggtctgat gaagttgtag cctttctgat
     8461 gaaaccttat agctattcta tgggtattct ggctctcttg gtagctggta caacagctaa
     8521 gtcattgact gactcagtaa accggagcat ggaaaaaacc aatcaaatca actatatgtc
     8581 aacattgttg gcagcaattg ttggtttgtt gatgttggca gctgatccta tcgaaagtgg
     8641 tctagctact ggattcttgg ggacaaaagg tttgctttca gccttccttg ctgcctttgt
     8701 tactgtagcc atctataagg tttgtgttaa gaacaacgtc actattcgta tgcctgacga
     8761 agttccacca aatatctcac aagtctttaa agatgtgatt ccattcactc tatctgttgt
     8821 ttctctttat gctcttgact tattagcacg ttattttgtt ggttctagtg tggcagaatc
     8881 aatcggtaaa ttcttcgcac cactcttctc agcagcagac ggataccttg gtattaccat
     8941 tatctttggt gcctttgcct tcttctggtt tgttgggatt catggtccat ctatcgttga
     9001 accagctatc gcagctatta cctatgccaa tgccgaagtc aacttgaacc ttctccaaca
     9061 agggatgcat gcagacaaga ttcttacttc tggtacacaa atgtttatcg ttaccatggg
     9121 tggtacaggt gcgacattgg tcgttccatt tatgttcatg tggttgacaa aatcgaaacg
     9181 taaccgtgca atcggacgtg cttcagtagt tcctaccttc ttcggtgtaa atgaaccaat
     9241 cttgtttggt gcacctcttg ttttgaatcc aatcttcttc attccattta tctttgctcc
     9301 aattgcaaac gtatggattt tcaaattctt tattgaaact cttggaatga actcattcac
     9361 tgctaatcta ccatggacaa ctccagctcc actaggtcta gttcttggaa ctaacttcca
     9421 agtgctatca ttcattcttg ctgcccttct aattgtggtt gacgtagtta tttattaccc
     9481 attccttaaa gtctatgatg aacaaattct tgaagaagaa cgttcaggta agtctaatga
     9541 tgaattgaaa gaaaaagttg ctgcaaactt caacactgca aaagcggatg ctattcttga
     9601 aaaagcgggt gtcgatgcag cacaaaatac catcactgaa gaaacaaatg tcctcgttct
     9661 ctgtgctggt ggaggtacaa gtggactcct tgcaaatgct ttgaataagg cagcagcaga
     9721 atacaatgtc cctgtgaaag cagcagcagg cggctatggt gctcaccgtg aaatgttacc
     9781 agagtttaat ctagttatcc ttgcccctca agttgcttca aactttgaag atatgaaggc
     9841 agaaacagat aagctcggta ttaaacttgc taagacagaa ggcgctcaat acatcaaatt
     9901 aactcgtgat ggaaaaggtg ccctagtatt cgtacaagag caattcgatt aaggctagga
     9961 actgtgaaac agtctcccat cgttacggaa atcgctatgg cgaatttcct aatcgcctta
    10021 ttaattcgtc ggtaaaaaga tatcgttttt acctcctcat gtcacaattc agtgacttgg
    10081 tacaagaagt gagatggaga agcatggctc actgactcct ctcctctcac ttttacttta
    10141 tttaaatcaa gaaataggtg aaaaaaatga caaaaacact tccaaaagac tttattttcg
    10201 gtggcgcaac agctgcttat caagcagaag gtgctacaca tactgatggt aaaggaccag
    10261 tagcgtggga taaatatctt gaagataact actggtacac tgcagaacca gccagtgatt
    10321 tttacaatcg atatccagtt gacctcaagc tagcagaaga gtatggtgtc aatggcattc
    10381 gaatttctat tgcctggtca cgtattttcc cgactggtta tggtcaagta aatgctaaag
    10441 gtgttgagtt ttatcataat ctatttgcgg agtgccacaa acgtcatgtt gagccctttg
    10501 taactcttca tcactttgac acgccagaag ctctccactc aaatggcgac ttcttaaacc
    10561 gtgaaaacat tgaacacttt gtagactacg cggctttctg ttttgaagaa tttccagaag
    10621 taaactattg gacaaccttc aatgaaattg gaccaatcgg tgatggtcaa tatttagttg
    10681 gtaaattccc tccaggaatt cagtacgacc ttgccaaagt cttccaatcg caccacaata
    10741 tgatggtgtc tcatgcacgc gcggtaaaat tgtacaaaga gaaaggctat aaaggggaaa
    10801 ttggtgttgt tcacgccctg cctactaaat atcctctaga tcatgaaaat ccagcagatg
    10861 ttcgtgcagc tgagttggaa gatatcatcc acaataaatt catcttagac gcaacttatc
    10921 taggtcgcta ttcagctgaa accatggaag gtgtcaacca tatcttatca gtcaatggtg
    10981 gtagtttgaa tcttcgtgaa gaagatttta cagcattaga agctgcaaaa gacttgaatg
    11041 atttcctagg aatcaactac tatatgagtg actggatgga agcctttgat ggagaaactg
    11101 aaattatcca taatggtaaa ggtgaaaaag gaagctctaa gtatcaaatc aaaggtattg
    11161 gtcgtcgtgt agctcctgac tatgtatcac gcacggattg ggattggatt atctaccctc
    11221 aaggtttgta tgaccaaatc atgcgtgtga agaaagatta tcctaactac aagaaaattt
    11281 atatcactga aaatggtctc ggctataaag atgagttcgt tgataacact gtttacgatg
    11341 atggtcgtat tgattacgtg aaacaacact tggagatctt gtctgatgcg attgcagatg
    11401 gagctaatgt aaaaggttac ttcatttggt cattaataga tgtcttctca tggtcaaacg
    11461 gttatgagaa acgttatggt ctcttctacg tagattttga aactcaagaa cgttatccta
    11521 agaaatcagc tcactggtac aaaaaagtag cggaaactca gattatagac tagtagaatt
    11581 agtcattaga tatagaattt tagtgagtca aaaagatgtt caaagatttt atccaatcta
    11641 tttatgaaaa agtttatatt ataaa
//
LOCUS       DAVHIT010000060        11570 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVHIT010000060 DAVHIT010000000
VERSION     DAVHIT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.061"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(174..254)
                     /locus_tag="VVN16_001498"
                     /pseudo
     CDS             complement(174..254)
                     /locus_tag="VVN16_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(298..2295)
                     /gene="metG"
                     /locus_tag="VVN16_001499"
     CDS             complement(298..2295)
                     /gene="metG"
                     /locus_tag="VVN16_001499"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5572850.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2419..3678)
                     /locus_tag="VVN16_001500"
     CDS             complement(2419..3678)
                     /locus_tag="VVN16_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572851.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3680..4381)
                     /locus_tag="VVN16_001501"
     CDS             complement(3680..4381)
                     /locus_tag="VVN16_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572852.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4365..5564)
                     /locus_tag="VVN16_001502"
     CDS             complement(4365..5564)
                     /locus_tag="VVN16_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5572853.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPATGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KTDEATN"
     gene            5850..7196
                     /gene="gor"
                     /locus_tag="VVN16_001503"
     CDS             5850..7196
                     /gene="gor"
                     /locus_tag="VVN16_001503"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU5572854.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            7393..7929
                     /locus_tag="VVN16_001504"
     CDS             7393..7929
                     /locus_tag="VVN16_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU5572855.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7980..8066
                     /locus_tag="VVN16_001505"
                     /pseudo
     CDS             7980..8066
                     /locus_tag="VVN16_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8135..8350)
                     /locus_tag="VVN16_001506"
     CDS             complement(8135..8350)
                     /locus_tag="VVN16_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU5572856.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8448..8768
                     /locus_tag="VVN16_001507"
     CDS             8448..8768
                     /locus_tag="VVN16_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572857.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8798..9136)
                     /locus_tag="VVN16_001508"
     CDS             complement(8798..9136)
                     /locus_tag="VVN16_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572858.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(9148..9867)
                     /gene="trmD"
                     /locus_tag="VVN16_001509"
     CDS             complement(9148..9867)
                     /gene="trmD"
                     /locus_tag="VVN16_001509"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5572859.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9857..10375)
                     /gene="rimM"
                     /locus_tag="VVN16_001510"
     CDS             complement(9857..10375)
                     /gene="rimM"
                     /locus_tag="VVN16_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU5572860.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10406..11194)
                     /locus_tag="VVN16_001511"
     CDS             complement(10406..11194)
                     /locus_tag="VVN16_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5572861.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(11298..11541)
                     /locus_tag="VVN16_001512"
                     /pseudo
     CDS             complement(11298..11541)
                     /locus_tag="VVN16_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
BASE COUNT         3319 a         2562 c         2101 g         3588 t
ORIGIN      
        1 tctacaacct caaaacagtg ttttgagctg acttcgtcag tttcatctac aacctcaaaa
       61 cagtgttttg agctgacttc gtcagtttca tctacaacct caaaacagtg ttttgagctg
      121 acttcgtcag tttcatctac aacctcaaaa acatgttttg agctgacttc gtcagttcta
      181 tctacaacct caaaacagtg ttttgagcaa cctgcggcta gcttcctagt ttgctctttg
      241 attttcattg agtataaata aaaaaacaga actagcctga actagtcctg tctactttta
      301 cccaatcaca cttccatttg gtacagctgg atcaactgtg agaagggtta atttgccatc
      361 atgttcagct gagagaatca taccctggct gacatatttt ttcatcattt tacgtggttt
      421 gaggttagca acgatttgaa ctttcttgcc gaccaattct tgttcatttg gatagtattt
      481 tgcaattcct gaaagaatct gacgatcttc tccatcacca gcatccaagc ggaattgaag
      541 caacttatct gaaccttcta ctttagacac ttctttgact tctgcgacac ggatttcaac
      601 cttgtcaaag tcttcaaact tgatttcatc cttgtttagt ttgagctcaa cttcgtccgg
      661 attccattct ttttcgactg ctggtttatt gccttccatt tgttccttga tataggcgat
      721 ttcttcttcc atatttagac gtggaaagat aggtgttcct ttggcaacta cagtcacatc
      781 tgctgggaag tcagccaaac tcaagttttc aagactagaa acttcttcca aaccaagttg
      841 agtcaaaact gcacgactag tttccatcat aaatggttca atcaagtgag caactacacg
      901 aatgctggct gccaagtggc tcatgacact tgccaattgg tcacgaagag cttcatcctt
      961 gtccaagacc catggtgcag tctcatcgat gtatttattg gtacgagaga tcagagtcca
     1021 gactgcttca agcgcacgtg gatagtcaac tgcttccatg tgtgtatgga agtctgcgat
     1081 tgattgttct gcaacctcag caagaacatg atcaaattca gtcacacctt ctacataggc
     1141 agggatttgt ccatcaaagt acttattaat catggaaacc gtacggttaa ggaggttccc
     1201 aaggtcatta gccaattcat agttgatacg gccgacatag tcttcaggag taaaggttcc
     1261 gtctgaacca actggaaggt tacgcatgag gtagtaacga agtggatcta gtccataacg
     1321 ctctaccaac atttcagggt aaacgacatt cccttttgac ttagacattt ttccgtcttt
     1381 catgacaaac caaccatggg caatcaaacg atcaggtaat ttaacatcca acatcataag
     1441 aaggattggc cagtagatag agtggaagcg aaggatatct tttcctacca tatggaagac
     1501 tgttccattc cagaacttgt caaagttacc atgttcgtct tgagcgtagc caagagctgt
     1561 cgcatagtta agaagggcat caatccaaac gtagacaacg tgttttggat ttgatgggac
     1621 aggcactccc catgtaaagg ttgtacgaga taccgccaaa tcttccaaac ctggctcgat
     1681 gaagttgcgt agcatttcat taaggcgacc atctggcgtg ataaattcag gatgagcttt
     1741 gaaaaattcg accaaacggt cttggtattt gctaaggcga aggaagtatg attcttcaga
     1801 aacccattca acctcatgac ctgatggagc aataccacca gtcacatttc cagcttcatc
     1861 acggaaaact tctgccagct ggctttctgt aaagaattct tcgtctgata ctgaatacca
     1921 accagagtat tcacccaagt agatatcatc ttgagcaagt aagcgttcaa agacctgtgc
     1981 aacaactttt tcatggtagt catcagttgt acggataaat ttatcgtatg agatatctag
     2041 taattgccag agttctttaa ctccaaccgc cattccatca acataggctt gaggtgtaat
     2101 accagcttct tccgctttct gctggatttt ctgaccatgt tcatcaagac ctgtcagata
     2161 aaatacatcg tagcccatca ggcgtttgta acgtgctagg acatcacatg cgatagttgt
     2221 gtaggcagaa ccgatatgaa gtttcccaga tggatagtaa atcggcgttg taatataaaa
     2281 atttttttca gacataattt ttcctttcca ggcaaatgaa acctgttttt ctaacacttc
     2341 attatatcac atttttaatg aatttcgata gggaaatcca tacaaaaaca agatagacga
     2401 gtgtccatct tgttgatctc attcataacg aagggcttca attggatcaa gtttcgatgc
     2461 cttgttggct ggcaagactc caaaaatcat accaacacta gccgaaactg caagactaaa
     2521 tagggcaact gggattgata ctccaacttc tataccttct attaaacctt gcagtaacaa
     2581 acctgctaag gcagttaaac cacttgcaat tgtcaagcca attaagccac ctaacaaggt
     2641 caaaatcatg gattcaatca aaaactgaat taaaatattg gcacgtgttg cgcccaaagc
     2701 cttacgaaga ccaatctcac gagtgcgctc tgtcaccgaa accagcatga tgttcatgac
     2761 accagttcct ccaacaaaga gagaaatccc tgcgatggaa ctaataatcg tcgtcataaa
     2821 actaaacgat tgttgaattt ctgcaaatac aacggactca tctgccacct ggtattctcc
     2881 ctgttgtaag cctgcaagct ctgtcatttt tcgtgccaat tctggaccca gagttggggt
     2941 taaactggta tcattcactc gaaagacaat attagctatt tcatctacat taaaattcgc
     3001 agcaagggag atattggtag taataggcaa gccaccaaac ccatatattt ttgatctttt
     3061 agcctccgga ctagtataaa ccccaatgac ccggtaacta aatccattga cttctacaac
     3121 cttgttaata gcctcttgag gagattcaaa taaactaatg gacaattcct catctagcaa
     3181 aatgacactt gcaaactctt tgaaatcttg ctctctcaga ctacgacctg caataatttc
     3241 attcttaaca gcgtccatgt aagttctgtt tccacctgtc aaattagcat tctcaacctt
     3301 tttatcttga taggtcaaga tggcattcgt tgaattggtt acatagtaac tatccactcc
     3361 cttcagttta gctgcctctt gaacccagga ttcttgcggt tttggcggtt caacaggaac
     3421 ttcctcttcc tttccagaaa ccgtaaaagc tgattgtttc tgagtaaaag acccatcttt
     3481 acttttttta ggagagaaaa agacgctaat atttttctga gatttagtca tatctttatt
     3541 gacttgacga gatagggaat cacccaaagc cataatcaca acaactgatg aaacaccgat
     3601 aataatccca atcatagtaa gcaaagaacg catcttgtga gccatgatag atgaaaaggc
     3661 aaatttcaga ttctgcatct tagttttcct cctttcctaa ctgagcactg tcagacgaaa
     3721 tgaccccatc ccgaatgaca atctgacgtt tggcataagc agcaatctca ggctcatgcg
     3781 ttaccatgat aatggttttt ccttctttat tcaaatcaac caataattgc ataatttggt
     3841 tacctgtttt ggtatccaag gctcctgtcg gttcatccgc taggataata gaaggattgt
     3901 ttaccaaggc acgcgcaatg gctacacgtt gcttttgacc accagataat tctgaaggta
     3961 aatggtgact acgttctgtc aattcaacct tgtctaaata ttcctcagcc aacttgcgac
     4021 gttttgaaga cgaaactcct gcgtaaatca agggcaattc tacattttgc agagcattga
     4081 gcttcgatag aagaaagaac tgctgaaaga caaaaccgat ttgttggtta cggaccttag
     4141 ccagttgttt ttcaccaagc ccagccactt cttgaccttc aagataatat tctccactgg
     4201 ttggtgtatc caacatgcca atcgtattca tcagagtgga cttaccagac ccagatggtc
     4261 ccatgatggc tacaaattca ccctcattca cttctagatt gatatttttg agaacctgca
     4321 attcttggtc accattacgg tagcttctga agatattttt tagactaatt agttgcttca
     4381 tcagtcttca cctcttttcc ttcttccaag gaagatgttg gattactgat gaccttagca
     4441 ccattcgtta aaccagaagt gatttcttga ttttctgcgt cagcatttcc caatgaaacc
     4501 tcaacttttt tagccttttg ttgttcatcc acaatccaga cataattttt actatcatcc
     4561 attactagac tgctaacagg aacaagaata gccttagttt tgcttttaac ctcaatgttg
     4621 acagaaaaac cctgtttcaa atcaccaacc tcgcctgtca catcaatagt ataagggtat
     4681 ttagaacctg tattattccc ggttgctgga ctagctgctt caccattgtt tttaggatag
     4741 tcagaaatat agcttaattt cccagtccat tttttatcag gatacacttt agaagtaaag
     4801 cttacttctt gacctacaga aaggttggct agattgtact cagacaattc tcccttgact
     4861 tgtaaatttt cattgctgac aatatgaacc ataacttgac tcgcccctgt tggagattta
     4921 gaaacattgc tattgacttc gactacagtt ccctctaggg tactgagaac agttgttgca
     4981 tccaattgac tttgagcctt gcttaattgc gctgcagcat ctgcacgcgc atcacgggca
     5041 tcacccaatt gagcatcaat agaagcaaca gaatttccag ccactggagt tgggctttgc
     5101 accgttgcat cttctcctcc tactggcgct ggtaactgtg gagcctgagc tgaagcggct
     5161 tcatttcgtg cttgattgag ttcattgata tgacgatctg ccttagctac tgctcgacta
     5221 gctgaatcat aggccgcctg cgcttctgaa ctactgtact tgactaaagc ctgcccttcg
     5281 ctgaccttat cgcccacaga aacaaggatt tcatctaaat cacccttact agcatcaaaa
     5341 taaacatatt gttcattttt tgctgttact gtccctgaca ataaaacaga ggatgccacg
     5401 cttccttcct tggcaacaac aagatgagta ggctcatctt ttacagcagt ctgagaaggt
     5461 tgtctaaaaa gtaaaatccc cccggcaccc aatacaacta cactcgcagc accgattgct
     5521 gcatacagtt gccacttttt agctttacca ttctttttct tcataatgaa actccttttc
     5581 ttttttacaa tactttgcta ttataccaaa tttccctcca gcaaacaatg cagttcagat
     5641 ggaaataatg gcagttcagg attaaacaat cgttcggaat tttgcttttc tattgtacta
     5701 gatatataat acactatctt gacttatttg gcaagctttt ttgcaatttt ttgtgcgtag
     5761 cagatttttg caatcaaatc aaaaaaagaa taaaatataa ctatcaaaaa agacactcta
     5821 ctatttaaaa gattacaaag gagttttcaa tgagagaata tgatatcatt gctatcggtg
     5881 gaggtagcgg aggaatcgct accatgaacc gtgctggtga acatggagcc aaagcagccg
     5941 ttattgagga aaagaaatta ggtggaacct gtgtcaacgt cggttgtgtt cctaaaaaaa
     6001 tcatgtggta cggggcgcaa atcgctgaga ctttccatca atttggagaa gactacggct
     6061 ttaagactac tgatcttaac tttgactttg caaccctacg tcgcaatcgt gaagcctaca
     6121 ttgatcgcgc tcgttcttct tatgatggta gttttaaacg caacggtgta gacttgattg
     6181 aaggtcatgc tgaatttgta gattctcata ctgtaagcgt aaatggtgaa ctgattcgtg
     6241 ctaaacatat cgtgattgct acaggtgccc atccaagtat tcctaatatt cctggtgctg
     6301 agctaggtgg ctcttctgat gatgtatttg cctgggaaga acttccagag tcaattgcca
     6361 ttctaggcgc tggttatatc gccgttgaat tagctggcgt actccacact tttggtgtca
     6421 agacagatct ctttgttcgc cgcgatcgtc ctttacgtgg ttttgattcc tacatcgttg
     6481 aaggtttggt caaggaaatg gaaagaacaa acttaccact tcacactcac aaagtccctg
     6541 tcaagttaga aaaaactact gacggcatta ccattcattt cgaagatggt actagtcaca
     6601 cagctagcca agttatctgg gctacaggtc gccgtccaaa cgttaagggc ttgcaacttg
     6661 aaaaagctgg agtgactctg aacgaacgtg gctttatcca agtggatgaa taccaaaata
     6721 ctgttgttga gggaatctat gctctaggtg atgtaacggg cgagaaagaa ctgactccag
     6781 ttgcaatcaa ggccggacgt accctatctg aacgtctctt taacggaaaa actactgcaa
     6841 aaatggatta ctcaactatt ccaactgttg tcttttcaca ccctgctatc ggaactgttg
     6901 gtttgacaga agagcaagct attaaagaat acggtcaaga ccaaatcaag gtttataaat
     6961 caagctttgc atctatgtac tctgcttgca ctcgcaaccg tcaagaatcc cgtttcaaac
     7021 tcataacagc tggttcagaa gaaaaagttg tcggacttca tggaattggc tacggcgttg
     7081 atgaaatgat tcagggattt gccgttgcta tcaaaatggg agcaaccaag gctgactttg
     7141 atgcaactgt ggcaattcac ccaactgcat ctgaagaatt tgtaaccatg cgttaatcga
     7201 aacaaaagaa gctgatataa atatgtctgc ttctttttta atgattttcg aatcaacaaa
     7261 aaaattatcc aacttgacta gtcacgaaaa tcggctgtta cagtttttgt taccttgttt
     7321 ttaaaaaatg gttgacatcg actcctatat gagtataata gttactatac attagaaaag
     7381 aggttaacta ttttgaaaaa agctcacgtt tatgctatcc ctgctattgg ggctgctctc
     7441 attgctgctt tggcacaaat cagtcttcca attggacctg ttcccttcac tctgcaaaac
     7501 tttgcaatcg gcttgattgc tactgtcttt agaccgagag aggctgtact ttctgctgga
     7561 ctctatcttc ttctaggtgc tatcggtctt cctgtctttg caggaggtgg agctggtttt
     7621 caggctttag ttggccctac tgcaggctat ctttggtttt atctcgttta ctctggactt
     7681 acttcctctc taaccaacag caagagtggt gttgttaaga tttttcttgc aaacctcttg
     7741 ggtgatgccc ttgtctttgt cggcgggatt ctcagcttgc atttcctagc tggaatggca
     7801 tttgaaaaag ctcttgctgt gggggttctt ccctttatca ttccagacct tggcaaactt
     7861 ctggctatta gttttattag ccgtccccta cttcaacgcc ttaaaaatca ggcttacttt
     7921 actaactaaa aaaggatatc gagttatcat gactcaatat ccttttcttt tattttgaaa
     7981 acttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc tgctcaaaac
     8041 actgttttga ggttgtggat gaaactgacg aagtcagtaa ccatacatac ggcaaggcaa
     8101 agctgacgcg gtttgaagag attttcgaag agtattagtt gcctttaaag gcatccacca
     8161 tcgtttgaaa ttcttcattt gagagagtaa tccctttgcc cattttagta tggtctggac
     8221 tccaagcacg aatatcaaac tttgcagggg caccattaaa gctcacacgg ttaatttcct
     8281 tggtccaacc tttttcgttt tcagaaagag tcaacaagtg ctcttcgatt tcaaatgtaa
     8341 attctgccat tttcttctcc ttttttagtt tcattagttt attcgtaaaa tcttgtagat
     8401 tttaggaaaa ttttatataa tattgatata aaagaaggga ggccaatatg agacataaat
     8461 tccagcaagt tctaaataaa atacatgatt ttttaaatgg atatgaccaa cctgaccaga
     8521 ctgaaaccaa ctcccttaca gccactattg aagaggctat ccagaaacaa accgctgttc
     8581 accttatctt gtctgagaca agctttacag gtgacatcat caaatatgat cagcaaggcc
     8641 agcaaattat cgtgaaaaat ttttccaaaa atgtgagccg gattatccgt ataagcgata
     8701 ttcaacgcct gcgatttgtc ccctcaactg tccaaacagc ccaaaaaaat agatttaaga
     8761 aagagtgaga tgtagttgct tcatcccact ctttttctta gcgaatttgt tcaaaatgta
     8821 aatgaactgc aatatgatct ccataaccac ttctttccaa gtcacgttgt aaacgatagg
     8881 aaatgtagtg ttctgcaatg gtaatgtaac ctgcgcccaa taaacgatgt tcaaccatag
     8941 attgaatcat actgatagtc gcacgttcca ccttggcttc ttgtaaatcc aaaactacct
     9001 tcttagtgac ttgagcaaga ttttgacgca aatcatctgt caaaacataa acagtttggg
     9061 ctgccttcaa gatggcttgg taaatcttat ctggattaaa ttcagcaatt tcgccattac
     9121 gtttgattac ttgcataggt ttctccttta ttctttgttt tctttgattt ctgccagcat
     9181 tttttcttct tctactgtca gttgataatg ttcaagtaaa tctggtctgc gctcgtaggt
     9241 tttctttaaa ctctcgtaca atcgccactg acgaatcttt tcatggtggc cactcatcaa
     9301 tacatctggc acgaccatgc ctcgataatc atagggacgt gtgtactgag gatattctaa
     9361 aagacctgaa gaaaaactat catcttggtg gctagactcc ttgccaatca cttctggaat
     9421 caggcgaact gtagcatcaa tcatggtcat agctgccaat tctccaccag tgaggacata
     9481 gtcgcctagg gaaatctcat ctgttaccaa ggtcttaatg cgctcatcat aaccctcata
     9541 gtgcccacag ataaagatta gctcttcctc ttgagccaaa tcttcagcat aagcctgatc
     9601 aaactgcttt ccagcaggat cgaggagaat aacgcgcgga tttttctttt caatagcatc
     9661 aaaggaatcg aaaataggtt gtgctctgag caacatgccc tgaccgcctc cgtagggctc
     9721 atcatctaca tgacgggcct tttcagcatt ttctcgaaaa ttatgatact ggatatccaa
     9781 gagccctttt tctcgagcct ttccaacgat tgagtgctcc agtggagaaa acatctctgg
     9841 aaagagggtt aaaatatcaa tcttcatcgt ctaacccttc taagatttcc acatcgaccc
     9901 gtttatttgg aatatcaaca ttgagaacca ctggtgggat ataaggtaaa agcaaatcac
     9961 gtttgccttt tcgtttgacc acccagacat cattagcacc tggttgcagg atttccttga
    10021 tggttccaac caagctatca ccctcataga cttccaaacc gataatctcg tgatagtaaa
    10081 attcaccatc gtctaggtca ttcaaatctt cctcagcgac cttgagactg tatcccttgt
    10141 acttttcgat agtattgata tggtacatat ctttgaattt aataatgtca aagttcttct
    10201 gtttacggtg gctagcgatg gtcactgttt ggacaaactg atctttttca tcaaacaaag
    10261 ccagctcagc tcctttttta aaccgttctt ctgcaaaatc cgtcacagac aagactcgca
    10321 tctcaccctg taatccctgc gtattaacga ttttcccaac attaaagtag ttcatcttgt
    10381 ctcctgtaat ctcctttttt ccatcttatt ctgacaattc tcgaataata gccgcaattt
    10441 tttccgattc tgaccattgt aaataatggt gattccctcc taaaatgagt ttagtattgg
    10501 aagtccaata ttctgattct ctgtactctt tttctctata agtctgacaa aaaataaata
    10561 cagggacatg agattctatc gacaaattct caaaatcttc ctcagtaatc tctccagata
    10621 tctgaaattc tggatcttga ttttccaact ctaagccttt ttcttgcatt aattcccaga
    10681 tttttttatt cgtttcagga ctaaatgttg cttgagttaa gttcttaaaa taaagttcag
    10741 gaccacactc gtcaatcagc ctcatctgct cttccatttc tggataagga ttttctgaaa
    10801 aatcagcaaa catgactttt ttagttgtcg gttcaattgc tactaaagtc tgacacttaa
    10861 ttggtttctc gagtaatttg caagctaaaa ttccactcca actatgtaca caaagtatat
    10921 attcagaaat ccctaattct tcaagtgctt cataaaccac atctgcaaga ttatctagat
    10981 tttttccagc ttggtcatga atcggactcc tacctgtgtt cggaaaatca attgtcaaat
    11041 aaccaattgt aggaggaggt ttttcaagta taagtgaaaa attttcataa cttggtagca
    11101 aacctgctcc gtttagacaa actagcactt tcttttgctt ttgataagca acagagagac
    11161 taccaatttc tgtagatact tcaaacctct tcataaagaa atccactgat tctatataat
    11221 gaattattaa aaatccttat cctttatttt atcacgttcc aaggattttc tcaagttgga
    11281 ggaaggggac aacatctcta ctttcccttc aagaatcctt ccaaatcatg tttatgttgg
    11341 taattaacgg ctgcggtttt gtctttctca aagatggttc tagttaggtc aatatgcttg
    11401 atagcagcaa tagcgacggt gtagtgaatg atatcagcca gttctctggc aagttcctcg
    11461 ttcgaatcct atcccttctt ttcgaccaga gcgcctattc aaaacctcgg ctacttctcc
    11521 gacttcctcc actaacttca taaagagacc ttcatcagtc cgagactgct
//
LOCUS       DAVHIT010000061        11197 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVHIT010000061 DAVHIT010000000
VERSION     DAVHIT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.062"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..981
                     /locus_tag="VVN16_001513"
                     /pseudo
     CDS             1..981
                     /locus_tag="VVN16_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1026..1667)
                     /gene="plsY"
                     /locus_tag="VVN16_001514"
     CDS             complement(1026..1667)
                     /gene="plsY"
                     /locus_tag="VVN16_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU5572862.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1803..3746
                     /gene="parE"
                     /locus_tag="VVN16_001515"
     CDS             1803..3746
                     /gene="parE"
                     /locus_tag="VVN16_001515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU5572863.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            4166..6637
                     /gene="parC"
                     /locus_tag="VVN16_001516"
     CDS             4166..6637
                     /gene="parC"
                     /locus_tag="VVN16_001516"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU5572864.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            6769..7791
                     /locus_tag="VVN16_001517"
     CDS             6769..7791
                     /locus_tag="VVN16_001517"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU5572865.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7847..8551
                     /locus_tag="VVN16_001518"
     CDS             7847..8551
                     /locus_tag="VVN16_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5572866.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            8616..9305
                     /locus_tag="VVN16_001519"
     CDS             8616..9305
                     /locus_tag="VVN16_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU5572867.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            9302..10225
                     /locus_tag="VVN16_001520"
     CDS             9302..10225
                     /locus_tag="VVN16_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU5572868.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            10240..10884
                     /gene="pcp"
                     /locus_tag="VVN16_001521"
     CDS             10240..10884
                     /gene="pcp"
                     /locus_tag="VVN16_001521"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU5572869.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            11052..>11197
                     /locus_tag="VVN16_001522"
     CDS             11052..>11197
                     /locus_tag="VVN16_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 family protein"
                     /protein_id="HEU5572870.1"
                     /translation="MSKKDKKIEIQVADAKVNVSKDSFEGYTLTIGKKVIGEIAELDG
                     QFAI"
BASE COUNT         3519 a         2000 c         2503 g         3175 t
ORIGIN      
        1 gagacctgaa gagaggacag aattagaata tcttcaagca gaaaatgagt acctgagagc
       61 ggagaatgcc atcctaaaaa agttaagaga actccgattg aaggaggaaa aagagaaaga
      121 agaaagacag aaattgttca agaattaatg actgagtttt cgttagttct tcttctaaaa
      181 tctattaaac tagctcgttg gacctactac tatcacttga aacagctaga caaaacagat
      241 aaggaccaag agcttaaagc tgaaattcaa tccatcttta tcgaacacaa aggaaattat
      301 gcttatcgcc gggttcattt agaactaaga aatcgtgctt atctggtaaa tcataaaaga
      361 gttcaaggct tgataaaagt actcaattta caagctaaaa tgcgacagaa acgaaaatat
      421 tcttctcata aaggagacgt tggcaagaag gcagagaatc tcattcaagg ccaatttgaa
      481 ggctctaaaa caatggaaaa gtgctacaca gatgtgacag aatttgccat tccagcaagt
      541 actcaaaagc tttacttacc accagtttta gatggcttta acagtgaaat tattgctttt
      601 aatctttctt gttcgcctaa tttagaacaa gtacaaacaa tgttggaaca ggcattcaaa
      661 gagaagcact acgagaatac gattctccat agtgaccaag gctggcaata tcaacacgat
      721 tcttatcatc ggttcctaga gagtaaggga attcaagcat ccatgtcacg taagggtaac
      781 agcccagaca atggtatgat ggagtccttc tttgggattc tgaaatcgga aatgttttac
      841 ggttatgaga agtcgtttca gtcgtttaag caattggaac aagctattat agactatatt
      901 gattactaca acaataaacg aattaaggta aaactaaaag gacttagccc tgtgcaatac
      961 agaactaaat ccttcggata aattaattgt ctaacttttg gggtgcagtt cacagaactg
     1021 gcattttatt ttttaggatc ttgatgggtt aggttcaatc cccaagggac caaattttca
     1081 gttttatttt tgatacgagc tatattgtcc ttatgacgaa tgataatcaa actagcaagt
     1141 gctaagataa tagcgatgaa gagagagtca tagttactca ggataaaacc aaaaagtgga
     1201 aagagcagaa ccccgataac agccgcgatc gatgctgtga cactagacag tgaaatcata
     1261 ctgccaagat agagagctcc aaagaagata atcgcaaggt agagacagaa gataggcgca
     1321 aatccgaaaa ttactccagc actggttgcg acagccttac cacctttaaa tcctgcaaag
     1381 atagggaagg tatggccgat aacagccaaa agtccaaaga tgagaggaga aacgccttgt
     1441 agatgaaaaa taatcggaag cagcgttgct agggttcctt tgaaaaagtc aatcacaaag
     1501 gttgccatac cagctttctt acctaaaatg cggaaggtat tggtcgttcc agtgttacca
     1561 gaaccatgct cgcgtagatt gatttgaaag aatacttgtc caatccagag accagatgga
     1621 atcgaaccca gcagataggc taggattaat aaaactattg taatcatact cctattatat
     1681 catgaattgg gaaagaaaag cagagaatct ctgctaaaat tgtcacatcg gagaaagaaa
     1741 aattttgcaa aatccttgga aaacctgtag aatagtaaag atgaacgaat aggaggttcc
     1801 ttgtgtcaaa aaaggaaatc aatattaaca attataatga tgatgctatt caggtgctag
     1861 aagggttgga tgcggtccga aaacgtccag ggatgtatat tggatcgacc gatggcgctg
     1921 gtcttcatca cctagtttgg gaaatcgttg ataatgcagt cgatgaagcc ttgtctgggt
     1981 ttggtgatcg tatcgatgta accatcaata aagacggtag tctaacggtt caagaccatg
     2041 gacgtgggat gccgacaggt atgcacgcta tgggaattcc aactgttgag gttatcttta
     2101 ccattcttca tgccggaggg aaattcggtc aaggtggcta taagacatca ggtggacttc
     2161 acggagtggg ttcttccgtt gttaacgccc tttctagctg gttagaagtt gaaattaccc
     2221 gtgatggcgc agtttacaag caacgttttg aaaatggtgg aaaacctgtc acgactttga
     2281 agaaaatcgg tacagcaccc aagtctaaaa caggcaccaa agttactttt atgcctgacg
     2341 cgactatctt ttctacgaca gatttcaagt acaataccat ttcagagcgc cttaatgaat
     2401 cagcctttct cttgaaaaat gtgaccttgt ctttaacgga caagcgaaca gatgaagcga
     2461 ttgagttcca ctatgagaat ggagtacaag attttgtttc ttatctcaac gaagataagg
     2521 aaatcttgac gccagttctt tactttgaag gggaagacaa tggttttcaa gtggaagtag
     2581 ccctccagta caatgacgga ttctcagata acattctatc ctttgtcaat aacgttcgca
     2641 ccaaggacgg tggaacgcac gagacaggac tcaagtctgc cattaccaag gtcatgaatg
     2701 actatgcacg taaaacaggt cttctcaagg aaaaagataa aaaccttgaa ggttcagact
     2761 atcgtgaggg actagcggcc gttctttcta tcttagttcc tgaagaacac ttgcagtttg
     2821 aaggacagac caaggataaa ctaggaagcc ccctagctcg cccagttgtg gatggaatag
     2881 tggctgataa gttgaccttt ttccttatgg aaaatgggga attagcttct aacctcatcc
     2941 gcaaggctat caaggcccgt gatgctcgtg aagcagcacg taaggcgcgt gatgagagcc
     3001 gaaatgggaa gaaaaacaag aaagataagg gcttgttgtc tgggaaattg accccagccc
     3061 aatctaagaa tcctgctaag aatgaactct atctagttga gggggactct gccggtggtt
     3121 ctgccaaaca aggtcgtgac cgcaagttcc aggctattct acctcttcgt ggtaaggttg
     3181 tcaatacagc caaggccaag atggcggata tcctcaaaaa tgaagagatc aataccatga
     3241 tttataccat tggtgcgggt gttggagcag acttctctat tgaagatgcc aactatgata
     3301 agatcattat catgaccgat gcggataccg acggtgccca tatccagacc ttgctcttga
     3361 catttttcta ccgttacatg cgtccgctag tcgaggcagg tcatgtctat attgccctcc
     3421 cacctcttta caagatgtcc aaaggtaaag gcaagaaaga agaagtggcc tacgcttgga
     3481 cggacggaga actagaagaa ctccgtaaac agttcggtaa aggcgctacc ctccaacgat
     3541 acaaaggact tggtgagatg aatgcggacc agctctggga aacaaccatg aacccagaaa
     3601 cacgtaccct catccgtgtc acaattgaag atttagcgcg cgccgaacgc cgcgtcaatg
     3661 ttctcatggg agataaggta gaaccacgcc gtaaatggat tgaagataat gtcaagttta
     3721 cgctagaaga agcgacagtg ttttaatgaa agaaaataat atgttagtaa actggaaatt
     3781 agagtcggat gtgaatgact atgtgaaaaa gcagtttgaa aatcttggtt tgaaaaaatt
     3841 gcaggattat taatgaagaa tctgctatga gtgcttatct caaagaggcg tttaagggag
     3901 cggctaagac acaaactaag actaattttg gaaaaccata ttttcatatt gaaaaataca
     3961 aacaggatag tttggttact tatcgtaact gaacacggga cgaattcccg taaaatatct
     4021 tttactgcat aaaaacctac tctacattct ttgaaaggag ttgaacacgc cctagatact
     4081 gtgtgaaaaa gataaattct cttgtgagtt tgcttacttc aagaattttc tattttcact
     4141 tggtatttta tgggctttgt atcttatgtc taacattcaa aacatgtccc tggaggacat
     4201 catgggagag cgctttggtc gctactccaa gtacattatt caagaccggg ctttgccaga
     4261 tattcgtgat gggttgaagc cggttcagcg ccgtattctt tattctatga ataaggatag
     4321 caatactttt gacaagagct accgtaagtc ggccaagtca gtcgggaaca tcatggggaa
     4381 tttccaccca cacggggatt cttctatcta tgatgccatg gttcgtatgt cacagaactg
     4441 gaaaaatcgt gagattctag ttgaaatgca cggtaataac ggttctatgg acggagatcc
     4501 tcctgcggct atgcgttata ctgaggcacg tttgtctgaa attgcaggct accttcttca
     4561 ggatatcgag aaaaagacag ttccttttgc atggaacttt gacgatacgg agaaagaacc
     4621 aacggtcttg ccagcagcct ttccaaacct cttggtcaat ggttcgactg ggatttcggc
     4681 tggttatgcc acagacattc ctccccataa tttagctgag gtcatagatg ctgcagttta
     4741 catgattgac cacccaactg caaagattga taaactcatg gaattcttgc ctggaccaga
     4801 cttccctaca ggggctatta ttcagggtcg tgatgaaatc aagaaagctt atgagactgg
     4861 gaaagggcgc gtggttgttc gttccaagac tgaaattgaa aagctaaaag gtggtaagga
     4921 acaaatcgtt attactgaga ttccttatga aatcaataag gccaatctag tcaagaaaat
     4981 cgatgatgtt cgtgttaata acaaggtagc tgggattgct gaggttcgtg atgagtctga
     5041 ccgtgatggt cttcgtatcg ctatcgaact taagaaagac gctaatactg agcttgttct
     5101 caactactta tttaagtaca ccgacctaca aatcaactac aactttaata tggtggcgat
     5161 tgacaatttc acacctcgtc aggttgggat tgttccaatc ctgtctagct acatcgctca
     5221 ccgtcgagaa gtgattttgg cgcgttcacg ctttgacaaa gaaaaggctg agaaacgtct
     5281 ccatatcgtc gaaggtttga ttcgtgtgat ttcgattttg gatgaagtca ttgctcttat
     5341 ccgtgcttct gagaataagg cggacgccaa ggaaaacctc aaagttagct atgattttac
     5401 ggaagaacag gctgaggcta tcgtaacttt gcaactgtac cgtttgacca ataccgatgt
     5461 ggttgtcttg caggaagaag aagcagagct tcgtgagaag attgctatgc tggcggctat
     5521 tatcggtgat gaaaggacta tgtacaatct catgaagaaa gaacttcgtg aggtcaagaa
     5581 gaaatttgca actcctcgtt tgagttcttt agaagacact gcgaaagcaa ttgagattga
     5641 tacagctagt cttatcgctg aggaagatac ctacgtcagc gtgaccaagg caggttacat
     5701 caagcgtacc agtccacgtt cctttgcggc ttccaccttg gaagaaattg gcaagcgtga
     5761 tgatgaccgt ttgatttttg ttcaatctgc caagacaacc cagcacctct tgatgttcac
     5821 aagtcttgga aatgtcatct acagaccaat ccatgagttg gcagatattc gttggaagga
     5881 catcggagag catctgagcc aaaccatcac aaactttgaa acgaatgaag aaatccttta
     5941 tgtggaagta ctggatcagt ttgacgatgc gacaacctac tttgcagtga ctcgccttgg
     6001 tcaaatcaaa cgggtagagc gaaaagaatt cactccatgg cggacctata gatctaagtc
     6061 tgtcaagtat gctaagctca aagacgatac agatcagatt gtagcagtgg ctccgattaa
     6121 actagatgat gttgtcttgg ttagtcaaaa tggttatgcc ctgcgtttca atatcgaaga
     6181 ggttccggtt gtcggtgcta aggcagcagg tgtcaaggct atgaatttga aagaagatga
     6241 tgtcctccaa tctggcttta tctgtaatac ttcgtccttc tacctcttga cccagcgtgg
     6301 aagcttgaaa cgtgtttcca ttgaggaaat tctagcaacc agccgtgcca aacgaggatt
     6361 acaagtcttg cgtgagttga aaaacaaacc gcatcgtgtc ttcttggcag gagcagttgc
     6421 agagcaagga tttgttggcg atttcttcag tacggaagtg gatgtgaacg accaaactct
     6481 gcttgtccaa tccaataaag gaacaatcta tgaaagccga ttgcaagact tgaacttgtc
     6541 agaacgcact agcaatggaa gcttcatttc tgacacgatt tcagatgaag aagtttttga
     6601 cgcttatctt caggaagtag ttactgaaga taaataagtg aaatgaagaa tcagttctaa
     6661 gagctggttt ttattggaga aattttctga aaattacaaa atatacttgc tattttagaa
     6721 aaatagtgta gaatataaga aataggtttt tagaataagg agtggaatat gacagtaacg
     6781 attgattggg aaaacctcgg tttttcctat atgaaattac cttatcgcta tcttgctcat
     6841 ttcaaaaatg gacaatggga tcaaggagag cttacagagg atgcaacttt gcatatttca
     6901 gagtcttctc caagtcttca ctatggacaa caagcatttg aaggtttgaa agcttatcgt
     6961 actaaggatg gcagtgttca actgttccgt cctgatgaaa atgctaaacg cctgcaacgt
     7021 acatgtgacc gtctcttgat gccacaagtt ccgacagaca tgtttgtaga agcttgtaaa
     7081 gcagttgtcc gtgcgaatga agaatacgta ccaccatacg gaataggtgg aaccttatat
     7141 cttcgccctc ttttgattgg tgtcggagat attatcgggg taaaaccggc agaagagtac
     7201 attttcacca tctttgctat gccagttgga aattacttta aaggtggttt ggtcccaacc
     7261 aatttcttga ttcaggatga gtacgaccgt gcagcaccaa atggtacagg tgcggctaag
     7321 gttggtggaa actatgctgc aagtctctta ccaggaaaaa tggccaagtc acgccatttc
     7381 tcagatgtta tctatctgga cccatcaact catacaaaga ttgaagaagt cggatcagct
     7441 aacttctttg gaattacagc tgataatgaa tttgtaacac cattgagtcc atctatcttg
     7501 ccatctatta ccaagtattc cttgctttat ttggcagaac atcgcttggg attaactcct
     7561 attgagggtg atgttccaat tgataatctt gaccgttttg tagaggcagg tgcctgtggt
     7621 acagcagcgg ttatttctcc aattggaggt attcaacatg gtgatgattt ccatgtattc
     7681 tatagtgaaa cagaagtagg tcctgtgacg cgtaaattat ataatgaatt gacgggtatt
     7741 cagtttggcg atattgaagc gccagaaggt tggattgtaa aagtagatta aaataaacca
     7801 aaggagattt tttatgaaat agaaaaagtg gctcttaaca gcaggagtgg tcctgagcac
     7861 gtcagctatt ttagtggctt gtggaaaaac tgataaagaa gcagatgcac cgacaacatt
     7921 ttcttatgtc tatgcagtag atccagcatc attgggctac agtatagcga ctcgaacatc
     7981 gaggacagac gttattggaa atgttattga tggtttgatg gaaaatgata aatacggcaa
     8041 tgttgctcct tctcaaaaag actatgattt gaacagtaca ggatgggctc caagctatca
     8101 agatccagcg tcttacttga atattatgga tccaaaatct ggttctgcca tgaaacacct
     8161 tggcattacg aaaggaaaag ataaggatgt tgtagctaaa cctggtttgg ataaatataa
     8221 gaaattgtta gaagatgctg tttctgagac cactgaccta gagaagagat atgaaaaata
     8281 tgccaaagct caagcttggt cgacagatag ttcattattg atgccaacag cttcatctgg
     8341 tggttttcca gttgtaagta acgtagtacc attctcaaaa ccatactcac aagttggtat
     8401 taagggggaa ccatatatct ttaaaggaat gaaattgcaa aaagatattg ttacaacaaa
     8461 agaatataac gaggttttta aaaaatggca aaaagaaaaa ttggaatcca atagcaaata
     8521 ccaaaaagaa ctagaaaaat acattaaata aggaatggta ttgatcttga taaaattttt
     8581 aaaatactgt cattttgaat ataaaggagt ttgatatgga gtggattaga ttaataggaa
     8641 tagcaatcat tgttgtgggt tttattttaa aatttgatac aattgcaaca gtagtcttag
     8701 ctggtttggt tacagcttta gtttcaggtg tttctctcgt tgaatttttg gagattttgg
     8761 gaaaagaatt tagcaatcag cgagtgctca cgatttttat ggttaccttg cctcttgtgg
     8821 ggctgtcaga aacctttgga ctcaagcaac gatcaatcga tttgattcga aagattaaag
     8881 gtctgacagt tggaaacttc tatacagttt atttctttat tcgagagtta gctggtttct
     8941 tttcaattcg tctaggagga caccctcagt ttgtcagacc tttggttcaa cctatgggag
     9001 aagcagctgc agagtctcaa ttaggtagaa agttaacaga ggttgaagat gagacaataa
     9061 aagcgcgtgc ggctgcgaat gaaaattttg gaaatttctt tgctcaaaat acgtttgtag
     9121 gtgctggggg agtcctcttg atagggggaa cattagatca gttaggctat gaaagcaatt
     9181 atgcagggat tgcttctaca tctattattg ttgctgttat aacacttatt gtagtgggga
     9241 tttacaatta tttatttgat aaaaaattga tatcaaaaaa gactagggga ggagaacaaa
     9301 aatgacagag ttggcaaagc aactattaga gttgacctat attgtgattg gttgtcaatt
     9361 tctccataca gcctattgta gttataaaga taaaacaaac ccagttcgac ttgggacatc
     9421 tgcattttgg actctattgt ctattacgtt tataggtggt tcctatatgc caaatatgag
     9481 tattggtatt attgtaatcc tattatcgct gttaacattg tttaagcaag tccgtatcgg
     9541 aaccttgcca tccttagatg aaatgaaagc caatattgaa tctaacaggt tgaaaaataa
     9601 aatttttatt ccagttatgc tgatggcaat acttgcgttg gtcttagcgc aaatgattcc
     9661 agaatttagc aagatttcga ttagccttgc cgccttgttt gctacaattt ctgttcttgt
     9721 gattaccaat agtcacccta agagtctgtt atcagaaaat aatcgaatga ctcagcaagt
     9781 ttcaacaagt gggattgttc ctcaattatt aggggctttg ggggctattt ttactgtagc
     9841 aggtgttggt gatgttatct ctcatctgat tagcggtatt gttccttcag atagtcgctt
     9901 tataggagtt ttggcctatg ttcttggaat ggttctattc acaatgatta tgggaaatgc
     9961 ttttgcagca ttcaccgtta ttacagcagg tgttggagtt ccctttgtat ttgctctggg
    10021 agctaatcca attgtggctg gtgctcttgc catgacagca ggttattgtg ggaccttatt
    10081 gaccccaatg gctgctaatt ttaacgctct accagcagca ttgatggata tgaaagatca
    10141 gaatggcgtt ataaaggctc aagcaggtgt tgctctagta atgattgtta ttcacatatt
    10201 cttaatgtac tttctcgcat tttagtaaag gaaaataaaa tgaaaatatt ggttacaggt
    10261 tttaatcctt ttggaggtga aaagattaat ccagctttgg aggctgtaaa attattacca
    10321 tctgagatta atggggctga agttcgctgg gtagaaattc caacggtttt ttataagtcg
    10381 tcagaagttt tagaggcaga aatattacga tatcaaccag atgctgtact ttgtattgga
    10441 caagcaggcg gcaggaccgg cttaacacct gaacgagtgg ctattaatca agatgatgct
    10501 cgcatacctg ataacgaagg caatcaacca attgatacac cgattcgtat tgatggagca
    10561 tcggcctatt ttagtagttt acctatcaaa gcgatggtac aagctatcaa aaaacaggga
    10621 cttccggcag ttgtatccaa tagtgcagga acctttgttt gcaatcattt gatgtaccaa
    10681 gctctttatt tagtggataa aaaattccct aatatgagag caggtttcat gcatattcca
    10741 tatatgatgg aacaagtagt aaataagccg aatactgcag gaatgagtct atgtgatatt
    10801 gtaagaggca tagaggtagc tattgaagct attgtagact acaaagataa ggatttgcag
    10861 ttagtaggcg gtgagactca ctgataacct aaaaaggata gtcaattatg cttgtttact
    10921 aactattaac tatgctaaat caattgaggt tgtttacata aaactctata tcagagaagc
    10981 ctgatataga gttttttctt gctagtttta ggattttctt gtaaaataga aaaagtgaag
    11041 agaggtatga aatgagcaag aaagataaaa aaatcgaaat tcaagtagcg gatgccaaag
    11101 ttaatgttag taaagacagt tttgaaggtt atacattgac tatcggtaaa aaagttatcg
    11161 gagaaattgc cgaattagac ggacaatttg ccattat
//
LOCUS       DAVHIT010000062        11043 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVHIT010000062 DAVHIT010000000
VERSION     DAVHIT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.063"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            11..733
                     /locus_tag="VVN16_001523"
     CDS             11..733
                     /locus_tag="VVN16_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU5572871.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKTKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VVN16_001524"
     CDS             730..1578
                     /locus_tag="VVN16_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU5572872.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VVN16_001525"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VVN16_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU5572873.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VVN16_001526"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VVN16_001526"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU5572874.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VVN16_001527"
     CDS             complement(3249..5957)
                     /locus_tag="VVN16_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5572875.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMETTANAYLTTAQTLDLAVMSNYGLDQADQEELRQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEEQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VVN16_001528"
     CDS             complement(5959..6660)
                     /locus_tag="VVN16_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5572876.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VVN16_001529"
     CDS             complement(6910..9321)
                     /locus_tag="VVN16_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU5572877.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGRI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDEKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9374..10066)
                     /locus_tag="VVN16_001530"
     CDS             complement(9374..10066)
                     /locus_tag="VVN16_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU5572878.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10601..10963)
                     /locus_tag="VVN16_001531"
     CDS             complement(10601..10963)
                     /locus_tag="VVN16_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572879.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
BASE COUNT         3218 a         2533 c         2061 g         3231 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa gactaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ccgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gcatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattagt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtt ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcacac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatgt caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggatagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccaag aaccttggta
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ctcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcgtcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaaccagc tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttgtcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgttcttctt ttttcaagag ttgcagacgt
     5221 accttgccat tatctgataa aatttgttca agctcttcct gatgttgctt agatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ttgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg tcttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgtctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat atgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tgtcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 gagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tatttagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagcactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct gcaacctcaa aacactgttt tgagcaacct gcggctagct tccttgtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttgaaaactg cttatattct taaatcctta ttaaaaggca gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatgtc tttaactgct ctgccaataa
     7321 tttatgggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggctagca cgcgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 gtgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataggtatt gccaatcgaa atcggcccat gttctggaga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctcc ctgagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttttcctcac ctttctgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgacc ggaaagaccg cctctaggag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgctcatact tttcatcaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttccg ccccttcctt agtaaaacgc tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcac
     8881 aagtgcctta ctaatattca gctgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaac atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagcccct atacggccaa atacttttgc tcctaaagaa ccattaccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga agctggttgt ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aagcctagaa atcaatccta
     9361 ggcttacaat tttttatttt ccaaggtagt attcagaaac aacgttcaat ttttcgtcga
     9421 attcgaatac caatggtggg aagttaggga tttccacgtc catgatctca tcatctgaca
     9481 aacctttgat gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt
     9541 ttttaccatc tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca
     9601 aagtcacttt caagttttca gcatctggga taactgagtc gtcaagtgaa gcgtaacgac
     9661 ggtctgtgtg agctgagtgc tcatcatcac ggtccatgtt tggaggcaat acatcgtatg
     9721 aacgacgcca gatgtgaact tgctcatcac caaattgttc agcagcttca gctttatttt
     9781 taccagtcaa accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc
     9841 acaattggtc agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg
     9901 aagtgtaagc ttggtcaaat ttgataccag cttctttgat caatttacca gcgtcaatcg
     9961 cttgttgtgt acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt
    10021 tgttccattc agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc
    10081 tttaaatttt ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa
    10141 aaagctagtc aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt
    10201 tttggttgtt tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt
    10261 ttgagttgac tcttttagtt tagtaaacga gtcattattt tccatagctg tgcctttgaa
    10321 accttcctca gttttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc
    10381 tgcataagtg cgtattgatt caatcgcatc gtctgttaca accggtgtat caaaagtgat
    10441 tgtttctttt gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatt
    10501 ctttgaactt tatgttccac atgcaactag aagagaaaga gataatagag ctactcctat
    10561 accgataaac ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt
    10621 tcttcaatgt tgcaaggaat agagatcctc cgataatagc aagaatcccc ccaacccatc
    10681 ctaagaatgg aatgagacta acttcaccag aaacaatcat tagtacagac ggagctgcac
    10741 ctacacgctt atctccttta tagtaaacaa tagcaatgat accaagaaca agatttgcaa
    10801 tcttcaaggc attcaagagt aaggcaacac ctgaaaaagc ccctgcagta gctcctccat
    10861 taagagttgt agcagctgta tttaccgctg ttcccaacaa gacaaaagga caaataagaa
    10921 gtaaaattcc tcccacaaga ccgacgatac cacttgattt cataataaaa tctccttttt
    10981 tatttttttc aaataaaatt gtatcatatt tgctttttaa aaaaagtatt ttctgatatt
    11041 ata
//
LOCUS       DAVHIT010000063        10946 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVHIT010000063 DAVHIT010000000
VERSION     DAVHIT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.064"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            165..329
                     /locus_tag="VVN16_001532"
     CDS             165..329
                     /locus_tag="VVN16_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5572880.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPAIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            413..979
                     /gene="amaP"
                     /locus_tag="VVN16_001533"
     CDS             413..979
                     /gene="amaP"
                     /locus_tag="VVN16_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU5572881.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            991..1161
                     /locus_tag="VVN16_001534"
     CDS             991..1161
                     /locus_tag="VVN16_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU5572882.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1200..1808
                     /locus_tag="VVN16_001535"
     CDS             1200..1808
                     /locus_tag="VVN16_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU5572883.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1839..2042
                     /locus_tag="VVN16_001536"
     CDS             1839..2042
                     /locus_tag="VVN16_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5572884.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2152..2424)
                     /locus_tag="VVN16_001537"
                     /pseudo
     CDS             complement(2152..2424)
                     /locus_tag="VVN16_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2628..2915
                     /locus_tag="VVN16_001538"
     CDS             2628..2915
                     /locus_tag="VVN16_001538"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU5572885.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3193..3963)
                     /locus_tag="VVN16_001539"
     CDS             complement(3193..3963)
                     /locus_tag="VVN16_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5572886.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSNIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3978..4826)
                     /locus_tag="VVN16_001540"
     CDS             complement(3978..4826)
                     /locus_tag="VVN16_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU5572887.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4823..6298)
                     /locus_tag="VVN16_001541"
     CDS             complement(4823..6298)
                     /locus_tag="VVN16_001541"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU5572888.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLERQIPSIYALRGKLKEDDQRRMVAAYLGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6621..7976)
                     /locus_tag="VVN16_001542"
     CDS             complement(6621..7976)
                     /locus_tag="VVN16_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5572889.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7986..8303)
                     /locus_tag="VVN16_001543"
     CDS             complement(7986..8303)
                     /locus_tag="VVN16_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5572890.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8320..8637)
                     /locus_tag="VVN16_001544"
     CDS             complement(8320..8637)
                     /locus_tag="VVN16_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5572891.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8652..9548)
                     /locus_tag="VVN16_001545"
     CDS             complement(8652..9548)
                     /locus_tag="VVN16_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU5572892.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9551..10813)
                     /locus_tag="VVN16_001546"
     CDS             complement(9551..10813)
                     /locus_tag="VVN16_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5572893.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNFHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLEHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3767 a         2194 c         1686 g         3299 t
ORIGIN      
        1 cgaaataatt gacttttcta tattaaagtt atataatagt aataatcaaa gaaattgatt
       61 ttttgatatt aaaataaaaa aggagggtag gcagtgttgt gatcaattat tgctggaggt
      121 cttattggtc tcttggcagg taaaatcact aaaaaagtag ttctatggga atcatcgcaa
      181 atgtattcgc tggtttagtc ggggcatatg caggacaatc tcttttaggt agttggggtc
      241 cagcaatcgc tggaatggct ttgctcccat ctattgtagg tgcagcgatt gtgattactg
      301 tagtgtcatt ctttacaggt agaaagtaaa cttttcgcca gtaaagttag caaactattt
      361 ttaaatcaat gacgggaaaa atagtttaaa tgttaaatcg aaaggattgt atatgtcaaa
      421 agcaaagaaa atatgtttca ttattttctg tattttaatc ttgacaattt tccttcctgt
      481 tttgatagat tatcatcaag ttagtgatct aggtattcat ctacttagct ggagacagaa
      541 ctccgtagtt gaattctatc ttgctagata tgtcttttgg gggacagtgg ttctatcaac
      601 tttagtttta ttatccattt tagttgtgat gttttatcct aaacgttact tggaaatcca
      661 acttgaaact aaaaacgata cattaaaatt aaagaattcg gcaatcgaag gttttgttag
      721 aagtttggtg agtgatcata gattgatcaa gaacccaact gttcatgtaa atttacgaaa
      781 aaataaatgt ttcgttcatg tagaaggtaa aattcttcct tcagacaaca tcgctgacag
      841 atgccaaata attcaaaatg aaataactaa tggattgaag cagttttttg gtattgagcg
      901 tcaagtaaaa cttgaagttg cagtaaaaaa ttaccaacca aaacctcaaa acaaaaagac
      961 tgttagtcgt gtgaagtaag gaagtaaaaa atggaatggc ttaaacaata tcgatatcca
     1021 attatcgctg gtctcatagg cgtatttctg gcttgtttga ttgtctcctt tggcttcttc
     1081 aaaacaatat ttgtattgat tttaggagca ctgggagttg cagctggatt atatatcgaa
     1141 aaaaactata tagataaata aaaaaataaa aattactaat ttaattaaag gagtttcata
     1201 tgtcaaacga aaaaaacaca aacactaacg tagaaaagaa agatgctact gttgtagctc
     1261 acgaaatcaa aggggaactt acttacgaag ataaagttat ccaaaaaatc attggtcttt
     1321 cactagaaaa cgtttcaggt cttttgggaa tcgatggtgg tttcttctca aatcttaaag
     1381 aaaaaatcgt taacagcgat gacgtaacaa gtggtgttaa cgtagaagtt ggtaaaacac
     1441 aagttgcagt tgacttaaac gttattgttg agtaccaaaa aaatgttcca gctttatatt
     1501 cagaaatcag agaaatcgta tcttcagaag ttgctaaaat gactgacttg gaaattgttg
     1561 aaatcaacgt aaacgttgtc gacatcaaaa ctaaagaaca gcatgaagca gactcagtaa
     1621 gccttcaaga tcgcgtatct gacgttgctg aatcaacagg agaattcact tcagaacaat
     1681 tcgaaaaagc taaatctggt cttggatctg gtttctcaac tgttcaagaa aaagttagcg
     1741 aaggtgtaga agctgttaaa ggtgcagcaa atggtgtagt atctcacgaa aacactcgtg
     1801 taaactaaga taaaataaat ataacaggag aaattatcat gtcagtagaa gaaaaattaa
     1861 atcaagctaa aggttctatt aaagaaggtg ttggaaaagc catcggtgat gaaaaaatgg
     1921 aaaaagaagg agcggctgaa aaagttgttt ctaaagttaa agaagttgcc gaagacgcta
     1981 aagacgctgt agaaggtgct gtagaaggtg ttaaaaacat gttgagtggc gacgataaat
     2041 aaggttaaaa gttactttat ctttttggta acattagtca aaagagtctg agtcgagatg
     2101 attctcagaa aacaaaaagc tagagattcc caattgcgga actctagctt tttaattttg
     2161 cctctttctc ttattatatt tcagcaggtt gttggccatg agtatgaatc ccatgtcaat
     2221 tctcacttga cgcttacctc tcagatgaca tctcttataa cccaaacaaa cctttatctg
     2281 cccaaagaca gatttcatat caatcttacg tttagcgaaa atttgtctac ccttggaaga
     2341 taaaagtgcc tgatattctt tagtttttaa acactggtaa cgttcattca tatacagtct
     2401 cttttgaggg gctgattcag gttcataatc gcagtcaaca ttgatttcaa ggctgtttgc
     2461 tttctatctc ccctttttca tgatatattt tcgtacaggt gcttcaatag tttctatagt
     2521 aaaatgaacc aaaaatagta cacgttgcgg tataatgttc ttatggcata ttcattagat
     2581 tttcgtaaaa agttctctcc tatttgtgag cgaacaggta tagtcaattg attgtcagtt
     2641 tgaagaccaa gagtcgtaag gcaaaggata tggcagaatc catccaaggg tggctggcac
     2701 aatttctagt gaacctcttt aaatctatta cgtttgactg tggaaaagag ttttcaaaat
     2761 ggaaagatat ttccaatcat cacgacagtg agagcttttt tgcgaatcta ggatgtccaa
     2821 gacagcggtg tctcaacgaa cattcaaata gacttttaag gtgtcatgac ttgccaaagc
     2881 agacagattt caacgaagtc agtcaagaat tttaggctgg acaaaaactc aatttcagga
     2941 gaaaatgaaa taaatattcc cacaataaaa cgcatagtat caaagttttt ccaatacctg
     3001 atattatgca ttttttaatt ttaaagactt tttcccagcc tcaagaaatc acatcccacc
     3061 taaatccttt ggataccaaa tacctcatca ggtattttct gagttggtaa gactgtctag
     3121 cttaatttga caaatggagg gcgttaaatc cagtttttac aggaagtcta tttaaatatt
     3181 ttcacactga ccttacaaaa tatgaaataa attatataaa gtcattccca tgacaaagaa
     3241 taacatccct ttaaaacagt atcttaccca attttcaggt aagacttttg atacaaccgt
     3301 ccccaatacc cctccgcata tagaagcaag aaaaataact ggaacaggtg ctaaatgata
     3361 ctgatttaaa cctgtaacca caacaattgt agccaaatga gataactggg aaaagaaaat
     3421 aattgcaaga gaatacatcg ttgcttcttt tatcgaaata gaaaaaaaca ccatcaataa
     3481 agatacattg agcggaccac caccaatccc taaaaaacta gaaattaagc ccaatagaag
     3541 accacaaatc atatagaatc ctatcttatc aaattgataa gtttttttaa aatctgttaa
     3601 aacaaatgat acaagtagca tcacaaatag taaaatcatt tgaacaatgg atactagccg
     3661 tactgataac tgagataaca aaacttgaaa aataagatga cctagaaatc ctcctaaaac
     3721 agaagaaaag gataccgtaa aaatcaatct ccattttatc tctttacttt gtgaaaatct
     3781 tttgattgtt gaaataatcg ccattataaa aacagagaaa ctagaataaa aagcaatatt
     3841 actgaccgaa tggtatccaa aactatccat aagtggctta attataattc caccacccat
     3901 gcctgatata gaacccaatc cactagctat tataatgatt agaaaataaa taatctcaac
     3961 cactttttaa gtccatccta ttctttcaaa catctaaaac ctagatggct actagtggaa
     4021 gtagaaatac atccatttct agcagctact cggtatcgat tacaataaga acaatgacat
     4081 aaaaaggaac ctcctctgat agcatactcc tcatcttgta tcccatattt tggtaactca
     4141 tagtcatctt cattaaaact agctaataag gtatatctcg gattgcgaca ccattcccaa
     4201 acattcccaa tcatttgata tagaccaaaa tcattaggta aaaattcata gacaggagcc
     4261 gttcctataa atccatctaa ggcagtattt tcataaggaa atcttccttg ccaagtattg
     4321 gcatgataat atccaccttc taaaagagta tctccccaag gatattcaga ttgtcttcct
     4381 cctctagcag catattccca ctgtgcttca gtcggtaaag acattccgct ccaattacag
     4441 aaagccaatg catcttctaa agcaacatga actactggat ggtcttctaa acccactaga
     4501 ttactattct caccgtaggg atgtttccag caagcgttcg gaacttgtaa ccaccacgga
     4561 gccccagcag gatgaggata tccctccctt tctgcctctg gaacgaacaa tataaaaaca
     4621 aaagaccact cttgtcgctc agctagtgtt ttatagcctg tttctttcac aaattgagca
     4681 aattcttgat tcgtaactgg agtatccgca atggaaaaat cctttacact aacagcgact
     4741 tgaggacctt caaaatcatc taaaaagccc tcttcagaat ttgttcccat cataaatgtc
     4801 ccacccttga tttttttcat tttcatgatt gactatcccc cgttttcgtt agagttggcg
     4861 ttattttaga aagttcaaca ggaactaact cttcatccac aacaaaacct tcttctctat
     4921 atctaagaaa gtctactaat tttttcttca tctgacgaac aataggttga tatttttcac
     4981 ttggatacaa atcattcatt tcatgcggat cttttttcat atcaaataac tgataatgat
     5041 ttaaaacagg aaaccagata aatttccact ggtctgtcaa gatatattga ctagaatctt
     5101 ttcctaaagc atgttcacca tgaaattctg ttcgccaccc ctcgtactgt ccaaataata
     5161 aattcttaac actccgtcca tctagttcat ctgtagtagt tcctcctgcc aaatccacga
     5221 gtgaaggaaa aatatcttga attttcacta attgcttgat tgttccccta ttaccagcta
     5281 tcaatccagc cggatcgtaa atgaacgaag gaatgtggat acttccttga taaggatatc
     5341 cctttctaaa cagataatgt tcaccaagct gatctccatg atccgaaaca aaccagataa
     5401 tcgtatcttt atcatgacga aattctttta gggcagtcaa aaagcgacta atttgatggt
     5461 ctatatgagt aatcaatccc aaataagcag caaccattct tctttgatca tcttccttta
     5521 atttccctct taaagcataa atagacggaa tttgcctttc taatacttcc caattcccga
     5581 tatgcaaatc gagaaattga ggcaagcgtt ccatatacat atcaaaatag tattttgggg
     5641 gatttaatgg agcatgtggt ttttcaaaag acattttgag aaaaaaagga accgttggat
     5701 ctcttctttg taaaaatgaa atggactcac tcacaaccca gtttgttgga tgtaacttct
     5761 catctttatc ccaaggtcta gcttcccaag aattacaatc cattccgtca tcaatcaaat
     5821 caacatcata accaacttta ccctttaaaa aggctagata gtcactagca taatcaaatt
     5881 gacttccata agccttatca tactttctat caacatgtag atacccatcg tgaagtaaga
     5941 catgatcaaa cccaagtctc tgtctggaag gaaaaacatg catcttacca atacactctg
     6001 tttgataacc catatcttta aagacttttg gaagagtatt agtaaaattc caaggcacct
     6061 catcttgata accaacacgt ccacttttat cttggtctaa accagtcaat aaagctgccc
     6121 tagcaggaac gcaagaagga actggagaat aggcattttc aaaattataa cctacactag
     6181 ccatcatatc taatgttgga gtgctaacta gcttatcctt actattcaag gataaggcgt
     6241 ctgctctcat ttgatctaca acaatcaaaa taatatttgg ctgttttgtc tgaaccataa
     6301 aatctccttt ctaatatggc aaaagaggca caagaagata tctaccttta ctgcacccct
     6361 ttctatatca atctctctat ataaaacaat aacattcttg ttatgtttta tataaaaatg
     6421 aactaaaata tgactaaatc gattaggaaa ttcaaatcat tttctagtac tgttttagaa
     6481 gttacagtgt actattccaa cttcaataaa ctataaacct ttgtctatta acaattttag
     6541 tggagataag aaatcctaca cctaactcat cttacacgta atctatttct attttatcac
     6601 aaaaaacgca agtaagacca ttaactcaat tcagttttat ctgccatttt cacaaatggg
     6661 aaataagtca agacactaat aatcaaacaa acaactgata agatgatggc acgccaatca
     6721 aatgctgtag agaagaaacc atataaaatt ggaggcatta cccaagtaac attttgtgta
     6781 acaggtgaaa caagacccca gcttgttgcc cagtaagcta ccgttgccat gaaaaccggg
     6841 ctaagtacaa atggtataaa tagcaaagga ttcaagacaa ctggtaaacc ataattgacc
     6901 ggctcaccaa tattaaacag aactggtgct agaccaagtt tagcaacttt tcgataatga
     6961 ctgtttcttg aaaaaattaa aatagcaagt actaatccta atcctccaaa ccagacaaac
     7021 gccccaaaag acccacttgt ccatatataa ggaatcggtt cacctttttg gaaagcatcc
     7081 agattcgcta acatagcaac tccaaatagc ccttccatga tgggagccaa tacatttcct
     7141 ccatggagac caaaaaacca gaataactta ttcaaaaaga tcatcagaat aactgcaaag
     7201 aaactttgag acaaacctag taatggcgtt tgtaacacct tgtaaaccca atcaatcaat
     7261 aagtcattgc taagtaaatg gaaaacataa gtcaagatgg ctactatata catcgccata
     7321 aatcctggaa tgatagaagt gaacggctta gcaatcgcag ggggaactga atctggtaac
     7381 ttgattaccc agttcttttt cattacttta cagaaaataa tagaggctaa aaatccaatc
     7441 atcatggctg taaagtatcc tctggcatta atatggtttc ctggaatcac attcccaata
     7501 gttaccatca gatttttacc atcaaatgct agattatcaa ttccttgtaa gatttgatct
     7561 aatttcacat ctcctacatt tgccaaaggg aaactctttg taactgtact tccaatcgaa
     7621 atgacaaacg aagcaagtga taccaaacca gcagaaactg tatcaacctt gtaaatctta
     7681 gcgatattca ctcccaagca atagatgaac aacaaggaaa caattggtat acttcccttg
     7741 aataccaaat tattgatgtc aacaagccac tgaaaggttt tcgtaatact ttctaggtga
     7801 aattgttgtg gtaaatccac tagaaaagca tttaataaca aagcaatgga acctgtcata
     7861 ataacaggca tagtccccac aaatgaatca cgtaaagcaa ctaaaaactt ctgattccca
     7921 actttcattg caattggaga gagtttttct tgcattttat ccaacattgt attcatacta
     7981 aactcctaat ttttttcaaa tcgttcgtat acttctacca cttctttcgc caagtcaaca
     8041 aaagtcagac tcgtcattaa atgatcttgc gcgtgtacca tcaagaggct aattgatgtt
     8101 tcttccccct gtgcctctct tgtcaacatt tctgtttgag ccttatgcgc ctgtaataag
     8161 gcagcattcg catcagctaa tcttcgattg gctttagaga aatccccttt ttttgctgcc
     8221 tgaatagcct ccatagcatt acttttggct tccccaccat acataattag ccccattgca
     8281 gattctaaat tactttcatt catagcattc tcctcaatct tagtctaata aactttcagc
     8341 taaatctaag actttagaac cattcatcat tccataatct gtcatcggaa taaccgccac
     8401 aggaatctgt ctatctttta gtttttcttg aaagtcccct agtaaatagc gaacttgagg
     8461 gcctaaaagc aacacattta cttcctttgt tgctacaatt tcttctgctt caggagctgg
     8521 aactgcaaaa atttctgcat ccaacccctt atcttctgct gccttttgca tctttgtcac
     8581 tagcagactt gtactcatac ctgctgcaca tgctaacata attgttactt tagccatagc
     8641 ttactccttt atcacctatt tactgaccta gccaagcgag atactgtatg tctcactcct
     8701 ctaattgcct tgctcaatgc ataaatatta tcgactgttg ccagtccccc atatcctgcg
     8761 tcacctatat gttgaatatc aactccacaa attttatttc taagtgcaat ttccttaata
     8821 gtatctgtat cagatgtttc ttggctagta ccaatagcac tcaatacaag accaccctta
     8881 ctatgaacga gatcaacgac ttcacgcaac tcttggtcat gaaaggctgg aacggttcca
     8941 actgctggaa caagtatcac atccgcacct gcttccaaca attgctctgc gacagaaagc
     9001 tctgccacag gctcattcac tcctgccccg tgcatcttac ctgcaataat caaaccagaa
     9061 aagttttcct tagcagtttg aacagcctta atgatttctc gattgctaac tcctgttcca
     9121 ggatttccag tcaaacagac aaagtcaaaa cctaactcct ctattcgatt caatgtttca
     9181 acactagcaa cacgacctgc aacaatttcc tgtgtttcct ctagcatctt tgcagatggg
     9241 tcaatcggtt ccaaatttac accaattgga caagcaacaa gctggtgtaa gcgatgaata
     9301 acatcatcac ctgaactatc caaagcataa attttaggct caaaaacatc aacacaattc
     9361 aataaaatca tatcagctcc aaaagctcta gcaatttcag aattagttat atccccaaca
     9421 aatgtctcac gagtaactac attttcagat agaacaactc taccttcact tgctaaaata
     9481 ctttgtttta attcttcagc attcatctgt aatatctcag atggatttgc actaattaaa
     9541 cgtttcattc tcaatcctcc aataacttaa accgtttttc aatagctatt acaaatggta
     9601 aataaagtaa gacatccaaa acaatcaata caatttgtaa taatgaacca gaaatacttc
     9661 ctgtagcaag taaaccactt ataacaggtg gcatagtcca acctggatct gtataagtta
     9721 gcggaaccaa acctgatgcc attgctcccc acgctactag taagttaaac attggagcta
     9781 agataaatgg aactagtaac aaaatgttca aaacaatagg aacgccaaac attgctggtt
     9841 catttatatt aaataatcca ggtataacag ttataggtgc caaagttttt agttgtttac
     9901 tagctttctt cttatgttct aaatatccta acgcaagaac taaacctatg gttgcaccac
     9961 cgccacctat gaatacaaaa ttatccataa atacgctagt aaaaatattt ggaagagttt
    10021 ctccagtttg ataagctact ttatttgcat ctagattagc taaccataac ggtttcataa
    10081 ctgcattaac aacatttcct ccgtgtaatc ctacaaacca aaaactacta ttaaggaaaa
    10141 ttaagataag aagaccaata acattatttc ctaaaagtcc aagtggacct cccaaagcta
    10201 cttgagcaat ttcatgaaaa ttaggtaaac ttaatttatc taaagttgca taaacaatca
    10261 accaaccaac aatagtcact gcaccaggaa taatggcact aaagctttta gatactgctg
    10321 gtggaacacc atccggcatt tttatctgta tattatgatt tataaaccat tggtagatat
    10381 agccattaat caatcctaaa atcatagcaa caaataaacc ttttgatgcc atgaaagcgg
    10441 taggcattcc tgctccagcc tctcctgtaa tgaaaggcgt aactgtaata aaagatgata
    10501 atgacacaat accagcagga ataccatcaa ctttgtattg tctagccata aagtaagcga
    10561 taccaaaact agctacaaga ccaagtaatc caaaactact atcaacaccc ttccataaat
    10621 agtcagcaac accaatatca cccagatatt tttcccatcc tggaatagga aaacttgcaa
    10681 taaccattag taatgaacct atcaacaata aaggtatagt tagagtaata ccgtctcgaa
    10741 tagaaaccaa cgctttatta ttacccaatc tagccgctac tggaagtaac ttttcttgta
    10801 ataatttttc cataaccttt tcctcttaaa tatattttat tatcaaaagt tatcaattat
    10861 ctttattttt tgacgtcaag ttgttatttg attatctttt gttatttata ttttacaact
    10921 ataagaaagc cttgtcaagg atttgt
//
LOCUS       DAVHIT010000064        10777 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVHIT010000064 DAVHIT010000000
VERSION     DAVHIT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.065"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            47..1975
                     /gene="glgB"
                     /locus_tag="VVN16_001547"
     CDS             47..1975
                     /gene="glgB"
                     /locus_tag="VVN16_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU5572894.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            1965..3107
                     /locus_tag="VVN16_001548"
     CDS             1965..3107
                     /locus_tag="VVN16_001548"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU5572895.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3097..4236
                     /gene="glgD"
                     /locus_tag="VVN16_001549"
     CDS             3097..4236
                     /gene="glgD"
                     /locus_tag="VVN16_001549"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU5572896.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4233..5666
                     /gene="glgA"
                     /locus_tag="VVN16_001550"
     CDS             4233..5666
                     /gene="glgA"
                     /locus_tag="VVN16_001550"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU5572897.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGNEVAVILPYY
                     DMVEAKFGSQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5725..6096
                     /locus_tag="VVN16_001551"
     CDS             5725..6096
                     /locus_tag="VVN16_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU5572898.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            6041..6265
                     /locus_tag="VVN16_001552"
     CDS             6041..6265
                     /locus_tag="VVN16_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5572899.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAE"
     gene            6379..7494
                     /locus_tag="VVN16_001553"
     CDS             6379..7494
                     /locus_tag="VVN16_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU5572900.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(7491..7937)
                     /locus_tag="VVN16_001554"
     CDS             complement(7491..7937)
                     /locus_tag="VVN16_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU5572901.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKIWEVKKEEPAKTSLWKKWFS"
     gene            8101..9405
                     /gene="eno"
                     /locus_tag="VVN16_001555"
     CDS             8101..9405
                     /gene="eno"
                     /locus_tag="VVN16_001555"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5572902.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            complement(9543..9680)
                     /locus_tag="VVN16_001556"
     CDS             complement(9543..9680)
                     /locus_tag="VVN16_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEU5572903.1"
                     /translation="MPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKI
                     F"
     gene            complement(9721..10777)
                     /locus_tag="VVN16_001557"
                     /pseudo
     CDS             complement(9721..10777)
                     /locus_tag="VVN16_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         3225 a         1902 c         2385 g         3265 t
ORIGIN      
        1 tagtataata aaaacataga aaacgcttac aggagaaagg gagtatatgg ataatagaga
       61 agcattaaaa acctttatga cgggtgaaaa tttttatctc caacattatc taggagcaca
      121 tagggaagaa ctaaatggag agtatggcta taccttccgt gtttgggcac ctaatgctca
      181 ggctgttcac ttggttggtg attttaccaa ctggattgaa aatcagattc caatggtaag
      241 aaatgacttt ggggtctggg aagtctttac caatatggct caagaagggc atatttacaa
      301 atatcatgtc acacgtcaaa atggtcatca actgatgaag attgaccctt ttgccgtcag
      361 gtatgaggct cgtccaggaa caggggcaat cttaacagag cttcctgaga agaaatggaa
      421 ggatggactt tggctggcac gaagaaaacg ttggggcttt gaagagcgtc ctgtcaatat
      481 ttatgaagtt cacgctggat catggaaaag aaattctgat ggcagtcctt atagttttgc
      541 ccagctcaag gatgaactca ttccttatct cgttgaaatg aactatactc atattgagtt
      601 tatgcccttg atgtcccatc ctttgggctt gagttggggg tatcagctta tgggttactt
      661 cgctttagag catgcttatg gccgaccaga ggagtttcaa gattttgtcg aggagtgtca
      721 tacccataat attggggtta ttgtggactg ggtaccaggt cactttacca tcaacgatga
      781 tgccttagcc tattatgatg ggacaccgac ttttgaatac caagaccata ataaggctca
      841 taaccatggt tggggtgccc ttaattttga ccttggaaaa aatgaagtcc agtccttctt
      901 aatttcttgc attaagcatt ggattgatgt ctatcatttg gatggtattc gtgtggatgc
      961 tgttagcaac atgctctatt tggactatga tgatgctcca tggacaccta ataaagatgg
     1021 cggaaatctc aactatgaag gttattattt ccttcagcgc ttgaatgagg ttattaagtt
     1081 agaatatcca gatgtgatga tgattgcaga agaaagttcg tctgcgacca agattacggg
     1141 aatgaaagag attggtggtc taggatttga ctacaaatgg aacatgggct ggatgaatga
     1201 tatcctccgt ttctacgaag aagatccgat ctatcgtaaa tatgacttta acctggtgac
     1261 tttcagcttt atgtatgttt tcaaggagaa ttatctcttg ccattctcgc acgatgaagt
     1321 ggttcatggc aagaagagta tgatgcataa gatgtgggga gatcgttaca atcaatttgc
     1381 aggcttgcgc aatctctata cgtaccaaat ttgtcaccct ggtaagaaat tgctcttcat
     1441 gggtagcgaa tacggtcaat tcctagaatg gaaatctgaa gaacagttgg aatggtctaa
     1501 cctagaagac ccaatgaatg ctaagatgaa gtatttcgct tctcagctaa accagtttta
     1561 caaagatcat cgctgtctgt gggaaattga taccagctat gatggtattg aaatcattga
     1621 tgcggataat cgagaccaga gtgttctttc ctttattcgt aagggtaaaa agggagaaat
     1681 gttagtctgt atctttaata tggtacctgt tgagcggaaa gattttacaa tcggactacc
     1741 cgttgcagga atttacgaag aagtatggaa tactgagttg gaagagtggg gaggcgtttg
     1801 gaaagaacat aatcaaactg ttcaaacgca agaaggacta tggaaagatt atgagcagac
     1861 cttaaccttt accctaccgg ctatgggagc aagtgtatgg aaaatcaaac gtcgcttgaa
     1921 atctactaaa accgtcacaa ataaaaacca aaaaggagta gaaaatgaaa aatgaaatgt
     1981 tagctttgat tcttgctggt gggcaaggaa ctcgtctcgg taaactcact caaagcatcg
     2041 caaaaccagc tgtgcaattt ggtgggcgct accgtatcat tgactttgcc ctatcaaact
     2101 gtgccaactc agggattcac aatgttgggg tcgttacaca gtatcaacca cttgctctca
     2161 acaaccatat tgggaatggt tcaagctggg gactagacgg tattaattca ggtgtctcta
     2221 ttcttcaacc ttattctgca agtgaaggaa atcgttggtt tgaggggact agtcacgcta
     2281 tttaccaaaa tatcgactat atcgacagtg tcaatcctga gtatgtcttg attttgtctg
     2341 gggatcacat ctacaaaatg gactatgatg atatgctcca gtctcataag gataataatg
     2401 ccagcttgac agtagcagtt ttagacgtcc ctcttaaaga agcaagccgt tttggtatca
     2461 tgaacacaga tgctaacaat cgtattgttg aatttgaaga aaaaccagct caacctaaat
     2521 ctacaaaagc ttctatggga atctacattt ttgattggca acgccttcgt aatatgttag
     2581 tcgctgctga aaagagcaag gttggcatgt cagactttgg taaaaatgtc attccaaatt
     2641 accttgagtc aggtgaaagt gtttatgctt acgaatttag tggttattgg aaagatgttg
     2701 gtactattga gtcactttgg gaagcgaaca tggagtatat ttctccagaa aatgccttgg
     2761 atagtcgtaa ccgtcaatgg aagatttact caagaaactt gatttcacca ccaaacttcc
     2821 tcggggcaaa tgctcatgtg gaagactcat tagttgtaga cggatgtttc gttgatggaa
     2881 ctgttaaaca ttctatcctt tcaacaggcg cgcaagttcg cgaaggagcg gaagtccttg
     2941 attcagttat catgagtgga gctatcattg gtcaaggagc taagattaaa cgtgccatta
     3001 ttggtgaagg tgcgattatt tctgacggtg tcgaaattga tggaacagat gaagtacaag
     3061 ttgtaggata taatgaagta gtgggggtag caacagatga agattgataa atattctgcc
     3121 attttaggaa atacagttgg ttttcacaat atgtcgacat tgacggacca ccgtccagta
     3181 gcaagtttgc catttggtgg gaaatatcgt ttgattgact tcccactttc aagccttgct
     3241 aatgcaggtg ttcgtagtgt ctttggtatt ttccagcagg ataatatcag ctcagtattt
     3301 gaccatattc gttcaggacg cgagtggggc ttatcaaccc ttcttagcca ttactatcta
     3361 ggaatttaca atacccgtgt agaaagtagt acagttggaa aagaatacta ccaacagctt
     3421 cttacttatt tgaaacgttc tggctcaaac caaacggttg ccctcaactg cgatgttctt
     3481 attaacattg atttgaacca agttttccac ctacatagta caacaaaaga gcctatcact
     3541 gtagtttata aaaaactagc taagaaagat atttcagaag taaatgcaat cttggatgta
     3601 gatgaaacag accatgttct ttctcataaa ctctttgata gcaagtcaac agctgaaacg
     3661 ttcaatatgt ctacagatat ctttgtcgtt gatacacctt ggttgattga acacttggaa
     3721 gaagaagcta aaaaagaaca tccagagaaa ttgcgctatg ttttacggga tttggctgta
     3781 aaagagggag ctttcgccta cgagtacacg ggctatctag ccaatattca ctctgttaaa
     3841 tcttattatc aagcgaatat cgatatgctt gaatcacaaa aattctattc tcttttctca
     3901 ccaaaccaaa agatttatac aaaggtcaaa aacgaagagc caacttacta tgctaataca
     3961 tctaaggtaa gcacttctca gtttgcctct ggtagtatta ttgaaggtca agtagctaat
     4021 tctgttctat cacgtaatat tcatgtccat aaggatagct tggttaaaga tagcctgctc
     4081 ttccctcgtg ttgttattgg agaaggggct caggtcgaat atgctatctt ggacaaaggg
     4141 gttgaagttg agcctggtgt tgtgattcga ggaactgcag aacatccagt tgtggttaag
     4201 aaaggtgcta aagtaacaga ggatattcat tcatgaaaat tttatttgta gcagcagagg
     4261 gtgcaccctt ttcaaaaaca ggtggtttgg gagacgtcat tggcgctctt ccaaaatcac
     4321 tggtaaaagc tgggaacgaa gttgcagtga ttttacccta ctatgatatg gtagaggcta
     4381 aatttggaag tcagattgaa gatgtgcttc attttgaggt gagcgttggt tggcgcagac
     4441 agtattgtgg aattaagaaa acagtattaa atggtgtaac cttctacttt attgacaatc
     4501 aatattattt cttccgtggt catgtttacg gtgattttga tgacggagaa cgctttgcct
     4561 ttttccaact ggctgccatt gaggctatgg aaaggattga ctttattcct gatcttctcc
     4621 atgttcatga ctaccataca gctatgattc ctttcttgtt gaaggaaaaa taccgttgga
     4681 ttcaagccta tgaggacatt gaaacagttt taaccattca taatttagaa ttccaaggac
     4741 aattttcaga aggaatgttg ggtgatttgt ttggagttgg ctttgaacgt tacgctgatg
     4801 gcacccttcg atggaacaac tgtctgaact ggatgaaggc aggtattctc tatgcgaacc
     4861 gtgtttcaac tgtttcacct agctatgctc atgaaattat gactagtcag tttggatgta
     4921 atttggatca gattcttaaa atggagtctg gtaaagtatc tggtatcgtg aatgggattg
     4981 atgctgatct ttataatcct cagacggatg ctcttttaga ctatcatttc aatcaggaag
     5041 atttgtctgg gaaagccaaa aataaggcaa aattgcaaga aagagttggc ttgcctgtta
     5101 gagcagacgt tccactggtg ggaattgttt ctcgtttgac acgtcaaaaa ggttttgatg
     5161 tggtggtcga aagtcttcac catatcttgc aagaagatgt tcagattgtt cttttgggaa
     5221 ctggcgatcc agcctttgaa ggagctttct catggtttgc tcagatttac ccagacaagc
     5281 tatcaacaaa tatcactttt gatgtcaaac ttgctcagga aatctacgct gcttgtgacc
     5341 tcttcctcat gccaagtcgt tttgaaccgt gtggcttgtc tcaaatgatg gctatgcgtt
     5401 atggaacctt gccattggtc catgaagttg gaggcttgcg agatacagtt cgcgctttca
     5461 atccaatcga aggaagcggt actggcttta gctttgacaa tctatctcct tattggttaa
     5521 attggacttt ccaaacagca ttggacttgt atagaaacca tccagacatt tggagaaacc
     5581 tacaaaaaca agctatggag agtgacttct catgggatac agcctgcaag tcataccttg
     5641 acttgtacca tagtttagtt aattaataga taaaatcgta tgatgacttc atacgatttt
     5701 taggctgttt agagaggaga actgatgtct caagtaaaag gcttgtgtgt tttggatgta
     5761 gatggaacct taatcctaga agaagtgatt gatcttttag ggagagaggc aggtcatgag
     5821 gcggaaattt cgcagattac aagtcgagca atgcgaggag agttggtctt tgaaagcagt
     5881 ttaagaaaaa gagtgtcctt gttggaaggt cttcctattt tggtctttga taacgtcttc
     5941 aactcaattc atctatcgct aaatgtccca gagttcatct ctattctcca aaagaatggc
     6001 atcctagttg gtctggtgtc tggtggattt acaccaatag ttggagagat tagcaaaatc
     6061 ccttggtatt gcctatttca ctgccaacca gcttgaagtc aaagaaggtc ttttaacagg
     6121 aaaattagtt ggacaaatta taagtcccca ggtcaaaaaa gaaactctgg aaaaatggag
     6181 aaagaaacta aaactttcta aagaaagaac ggtggcaatc ggtgatgggg tcaataatct
     6241 attaatgttg aagtcggcgg agtgaggaat cgccttttgt tccaaagaaa tgctcaaaaa
     6301 agaaatacca catcatgttg acaagaggga ttttttagaa gttcttcctt tgattgactg
     6361 tttagaatga gggaaaatat gaagattgta attgcaccgg attcgtttaa ggaaagcttg
     6421 acagctcaac aggtagctga agcaataaaa agaggcttcc aacaatcgat agcagatgta
     6481 gaatgtctcc tctgccctgt tggtgatggg ggagaaggta ctgtagatgc tatccgacat
     6541 tctcttgacc taaaagaaaa atggatccaa gtgacagacc cttttggaca aaaagaagcc
     6601 atgcgctatt ttcaaaaagg ggaactggca ctatttgaag tagctgactt ggttggtctt
     6661 ggaaaaattc cgctagagaa acgaaatcca cttcaaatcc aaacttgtgg tattggagag
     6721 ttgattctcc atctcatttc taaagggatt aaagatattt atatcggtgt tggtggcacg
     6781 gccagtaatg atggaggact ggggattgct gctggtttag gttatcaatt ttatgatagg
     6841 gatggaaatg tcttgcctgc ttccggtcaa tccttattaa acttagcttc tgtttcaaca
     6901 gaaaattgct ataaaattcc tgaaggtgtt caaattcata ttctagcaga tgtcgtgagt
     6961 cccttatgtg gtcatcaagg tgcgacttac acttttggca atcaaaaagg tctacatccg
     7021 actatgtttg cagtcgtaga tcaggcgatc caagattttt atgaaaaatt ctcacctgca
     7081 acattagaaa ttaaaggagc aggagcaggt ggaggccttg ctggtggttt gtgtgccttt
     7141 gctcaggcaa gtatcgtgtc tggaattgat acctgcttgg acttaatcaa ctttgataag
     7201 aaagtttcag atgctgactt ggttgttgtt ggagaaggaa gacttgatag tcaaagcttt
     7261 gctgggaaag cgcctatagg tgttgcaaaa agaacccctg ttggagttcc tgttattgct
     7321 atttgcggta gtcttgctga agatttacct cccctaccat ttgaaaatat ccaagctgtc
     7381 ttttctattt tggagaaaag tgaaccttta gaagatagtt tgaaaaatgc cagtctctat
     7441 ctggagcgca cggctgccaa tattgggcac ttattaaata tgtgcaagat ttagctaaac
     7501 catttcttcc ataaggatgt tttggctggt tcttcctttt taacttccca aatttttgga
     7561 aaagcaagtc gaaggtcttt ttcttctact tgaactggtg cattgctatg aataaccagg
     7621 ccgaaaggag aagtaatatg ctcttcagat actatggtgg cttgactatc agtttctttt
     7681 gcttctttta agtagaaaac ttgcttatca aattcgatag ttggtgaaat cttcacaaat
     7741 agtggctctg ctttttcctg aaggttttct aaaataaata aaaagccttt ttctaactga
     7801 gggctatttg ctgtgtcaat atctacatat ccaagaactc tttcctcaaa agtaccaaga
     7861 tagcgtcttt gctcatccgg atttattttt aacccaccat gagctttttc aagtaattgt
     7921 tttgataaat ctgtcatgaa aacagtatat cataaaagtt agaataaaac agacaaaaga
     7981 aagcgattac tttataaagt taaggaaaag ttgcacaaag ataacgtttt ttcttgaaaa
     8041 aggaggggtt ttaaggtatt atagaggtgt aaaaaattta actcataagg agagtaaaaa
     8101 atgtcaatta ttactgatgt ttacgctcgc gaagtcctag actcacgcgg taatccaaca
     8161 cttgaagtag aagtttacac tgaatcaggt gctttcggac gtggtatggt tccatcagga
     8221 gcttctactg gtgaacacga agcagttgaa cttcgcgacg gtgacaaatc tcgttacggt
     8281 ggtcttggta cacaaaaagc tgttgacaac gtaaacaaca tcattgctga agctatcatt
     8341 ggctacgatg tacgtgatca acaagctatt gaccgtgcta tgatcgcact tgacggtact
     8401 cctaacaaag gtaaattggg tgcgaatgca atcctcggtg tgtctatcgc tgtagctcgt
     8461 gctgctgctg actaccttga aatcccactt tacagctatc ttggtggatt caacactaaa
     8521 gttcttccaa ctccaatgat gaacatcatc aacggtggtt ctcactctga cgctccaatc
     8581 gctttccaag agttcatgat cttgccagtt ggtgcgccaa catttaaaga agcccttcgt
     8641 tacggtgctg aaatcttcca cgctcttaag aaaatcctta aatcacgtgg tttggaaact
     8701 gccgtaggtg acgaaggtgg attcgctcct cgtttcgaag gaactgaaga tggtgttgaa
     8761 actatccttg ctgcgattga agctgctgga tatgtaccag gtaaagacgt atttatcgga
     8821 tttgactgtg cttcatcaga attctacgat aaagaacgta aagtttacga ctacactaaa
     8881 tttgaaggtg aaggtgctgc tgttcgtaca tctgcagaac aaatcgacta ccttgaagaa
     8941 ttggttaaca aatacccaat catcactatt gaagatggta tggatgaaaa cgactgggat
     9001 ggttggaaag ctcttactga acgtcttggt aagaaagtac aacttgttgg tgacgacttc
     9061 ttcgtaacaa acactgacta ccttgcacgt ggtatccaag aaggtgctgc taactcaatc
     9121 cttatcaaag ttaaccaaat cggtactctt actgaaactt ttgaagctat cgaaatggct
     9181 aaagaagctg gttacactgc tgttgtatca caccgttcag gtgaaactga agattcaaca
     9241 atcgctgata ttgcagttgc aactaacgca ggacaaatca agactggttc actttcacgt
     9301 acagaccgca tcgctaaata caaccaattg cttcgtatcg aagaccaact tggtgaagta
     9361 gctgaatatc gtggattgaa atcattctac aaccttaaaa aataaaatag ttcagtgaac
     9421 tattttatcc cgaaccatga aattcaaaag ttcggccgtt gatttaacaa cgtttctagc
     9481 ccctcggatt ttatccgaag ggctattttt gtatttagag gacaaaaaag ggacaaaact
     9541 ttttaaaaaa tctttttcat aactttatcc agtacattga ttgcttcatc tttcatgttc
     9601 tttgtgacgt gggtgtagat ggaagtagtg acttcagagt cggaatgacc aaccctgtcc
     9661 gtgattgttt ttaaaggaat tttattggct ctttgtcaac tgtagtgggt tgaagaaaag
     9721 ctaagcttga gaaaggacaa attttgtcct ttcttttttg atattcaggg cgataaaaat
     9781 ccgttttttg aagttttcaa agtttcgaaa accaaaggca ttgcgcttga taagtttgat
     9841 gagattattg gtcgcttcca gtttggcatt agaatagtgt agttgaaggg cgttgacaat
     9901 cttttcttta tctttgagga aggttttaaa gacagtctga aaaataggat gaacctgctt
     9961 aagattgtcc tcaataagtc cgaaaaattt ctctggttcc ttattctgga agtgaaacag
    10021 caagagctga tagagctgat agtggtgttt caagtcttgt gaataactca aaagcttgtt
    10081 taaaatctct ttattggtta agtgcatacg aaaagtagga cgataaaatc acttatcact
    10141 cagtttacgg ctatcctgtt gaatgagttt ccagtagcgc ttgatagcct tgtattcatg
    10201 ggattttcga tgaaactgat tcatgatttg gacacgcaca cgactcatag cacggctaag
    10261 atgttgtaca atgtgaaagc gatccaacac gattttagca catgggaaaa gttgtctagc
    10321 taagtcatag taaggactaa acatatccat agtaatgatt ttgacgcgac atcggacggc
    10381 tctatcatat ttaagaaagt gatttcggat gacagcttgt gttcttccct taagaacagt
    10441 gatgatattg agattgttaa aatcttgcgc aatgaagctc atctttcctt ttgtaaaagc
    10501 atactcatcc caagacataa tctcaggaag acaagaaaaa tcatgtttaa agtggaagtc
    10561 attgagcttt cgaatgacag ttgaagttga gatggaaagc tgatgggcaa tatcagtcat
    10621 agaaatcttt tcaattaact tttgagcaat cttttggttg atgatacgag ggatttggtg
    10681 attcttcttg acgatagaag tttcagcgac catcattttt gaacagtgat agcacttgaa
    10741 tcgacgcttt ctaaggagaa ttctagtagg catacca
//
LOCUS       DAVHIT010000065        10748 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVHIT010000065 DAVHIT010000000
VERSION     DAVHIT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.066"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(104..1528)
                     /locus_tag="VVN16_001558"
     CDS             complement(104..1528)
                     /locus_tag="VVN16_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU5572904.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2072..4351)
                     /gene="pulA"
                     /locus_tag="VVN16_001559"
     CDS             complement(2072..4351)
                     /gene="pulA"
                     /locus_tag="VVN16_001559"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU5572905.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHFLVVVNAKSDPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4470..6428)
                     /gene="ligA"
                     /locus_tag="VVN16_001560"
     CDS             complement(4470..6428)
                     /gene="ligA"
                     /locus_tag="VVN16_001560"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5572906.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6521..7699)
                     /locus_tag="VVN16_001561"
     CDS             complement(6521..7699)
                     /locus_tag="VVN16_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5572907.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7779..8642)
                     /locus_tag="VVN16_001562"
     CDS             complement(7779..8642)
                     /locus_tag="VVN16_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU5572908.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLDHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8645..10546)
                     /locus_tag="VVN16_001563"
     CDS             complement(8645..10546)
                     /locus_tag="VVN16_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5572909.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALLIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQTQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3225 a         2369 c         1912 g         3242 t
ORIGIN      
        1 gtttcaatct attataatac caaaaagttc gtattttttc tataattatt attattttat
       61 agcaaaaaat aaaaccagga aaacaatttc ctgattttac acgttatttc acatcaaaaa
      121 caatggattt gacatttgtc atcgcttcaa tgctatattt aattccttgc actccagctc
      181 cagaaccttt gacaccaagg aatgggaaat tatctggacc acgctgggtt ttattattaa
      241 tgtggactgt acctacttca agtttttcag caatttcaaa ggcttttttg aaatcatttg
      301 taaaaactga tgattgaagg ccgaattcag attcgttggc aaaggcaata gcctcctcta
      361 cactagccac acgaatgatt ggtaaaacag gaccaaatgg ctcttcccat gccactttca
      421 tatcttttgt aacttggtca aaaagcactg gccagaggag attgccctca cgtttgattg
      481 gtgtaagagc ctgagcctct ttttcttgtg catcctcaat caagccccaa atgaagtcgg
      541 ctgaagcatt gtcaataaca ggtgtaatat cagcattgtc aaatggatca ccaactgtta
      601 atttagaaac ttcttcctga agcaaagtag ctaatttatc tgctacactt tcgagaacaa
      661 tgacacgttt aatggccgtg caacgttgtc ctgagtagct aaaggctccc gcaacaattt
      721 gcttggcagc atgttccaaa tctgcatctt ctagtacaag agctgcatct ttcccaccaa
      781 gttccaacat gataggacgc ataccagcta aacgaccaat acgttctcca ataggagttg
      841 aacctgtaaa gttgatgaag ttgacttctt tgtgctcaat gatataatcc ccaatttctg
      901 aaccacgacc tgtaatggtg ttgaaaacac ctgccggaat ccctgcttct tcaaatgctt
      961 tagccaacaa gagtccagaa atggaacctt gtgttggtgg cttaaacatg accacattcc
     1021 ctgcaatcaa ggcaggtgca attttagaag cagataaatt aactggataa ttaaaaggag
     1081 caatcgctag cacgatacca actggttcac gacggacaac agccagtttg tttttacttg
     1141 ctgcctcaaa accaccacct tccattgctt gtccagtgat acggagacct tcctcagcag
     1201 cataacgaat caagtctgct gtacgcacta cttctccaat tgctgcttta atcccttttg
     1261 ctacttcttt ggcaaggata gtaccaattt tttccttatc gcgttctaaa atagctgctg
     1321 ttttatgcaa ataagccgca cgttcaattg ctgataaagc tcgccatgct ggcagggctg
     1381 cacgcgcagc ttgcatagcc tcatcagctt cagtctgagt catggctgga actgtaccca
     1441 attcttcttg attgattggt gagtaaatcg taatttcttg ttcagatgat ttccattttc
     1501 catttactaa attctgatat cttgtcaaat tcttgtcctc ctgaaaatga ttaagatata
     1561 ttttaccaaa ttttcagaaa atcacaaccc tttttgcaaa atttttgaga ttattttcac
     1621 aaacttgatt tttcaaagta tactcaataa aaataaaaaa aatccactac gtcaaggcga
     1681 ggctaatgtg gtttgaagaa attttcgaag agcgtgaatg agtatcatct atagtaaaat
     1741 aaaaaactga acaatttggt tggggacagc caaaccaatt tctcacaatg tttcagaaac
     1801 aagggtgtgc tactccaatt tcggcctact ataactgtca tagattgctg aaacaaagtc
     1861 taggtaaaag tcttcataat aaaaagacct cctatcaaat gttcaaaaac tttgatagga
     1921 ggtcttgttt cgtgaaaata tttatcaaat tttctataca agtgagctgt tagccaggtt
     1981 ctttctattc tttcaatttc aatgaatgga ttttttacca atactcataa ctgggaattt
     2041 ttctgtctaa aaatagcgag atagatggta tttataaaac actcaagaca gctagactaa
     2101 tatcatttaa aacattatct tcttttgagc gactgttggt taccaacata gctaaatttc
     2161 ctgcattttc aaattgatag gggtctgatt tagcattcac aaccaccaag aagtgttctt
     2221 tgccgtgaac ttcatagata aggtagccgc tatgttcaat cgcagaatgc acaaagacat
     2281 gatggtaaat ttcatcatag ctagagtaag aaaaggcacc agtttttgtc ttcaatcgga
     2341 tgacttgacg gataaactca atactgtctt gacgctcatt aatcaagttc cagttcactt
     2401 ggttcacact gtcaggagca ttatagctat tcatcgcacg ctctctatca tcatgggtta
     2461 actcaccatt ttcaccagtc gcaaccagtt tggtacgacc aaattcttga ccgatttcca
     2521 taaaggccat cccctgcatg agcagattca tggctgtggc agtttcgacc ttgcgcatga
     2581 tttgctctga actttggtct ggatgaaggg ttgccaataa atcgtgaaga ttgtaattgt
     2641 catgggcttc tacatagtta agcacctgat ttggatgtgt atagcttcct aattcacgac
     2701 ttcctaggat tgctttagct agaattggct ctgtcgcagc accactgaca aaacctgact
     2761 tgatagcacc ataaacttct ccccctttga cagcatcgcg ctgattgtca ttaaagaaac
     2821 caatatttgg catctggtag gcattgtcct tcttggcctt atcataaggg gcaagacctg
     2881 ttcccatatc ccatccttct ccatagagga taatgttgga gtcgatttca tccaagcttt
     2941 gacgaatcat ctgcatggtc ttgacatcat gaatccccat caagtcaaaa cggaagccgt
     3001 caatattata ttcctgcacc cagtatagaa gagaatcaat catatacttg cgaaacattt
     3061 cgtgttcact ggctgtttca tttccaacac ccgttccatt ctggaaggta ccatctggat
     3121 tcatacgata atagtaatca gggactgttg tttggaatgg tgcatcaaca actgagaagg
     3181 tatggttata gactacatcc ataatgactc caatacccgc atcgtgataa gcttgaacca
     3241 tcaccttcaa atcacgaatg acctgagctg gatcatctgg attagttgaa aaactagttt
     3301 ctggcgcgtt atagttttgt ggatcataac cccagttgta ggttacattt ccatcctcat
     3361 cgtattcttt atgacggtct gcaattggtt gcaattgaac ataattgtag cccagcttct
     3421 tgatgtaatc aaaagcagtt gactggccgt attggttaac tgttccagcc tgagcagcac
     3481 ccaagaaagt tcctcgaaga tgttcatcta cacccgatgt aggtgattta gtcaaatcac
     3541 gaatgtgcat ttcacagata actgccttac atggattttc caagcgccaa gtagcctccg
     3601 aaccgtgctt aacctcgaag ttttcaactt gcttttctac atggctcaga atagctgaac
     3661 gtttgccatc agggctggtc gcgattgtat aaggatcacg tgtcagtgtt tggtgatgag
     3721 ggaattggac ttgatactga taagtcttac ctaccaaatc ttcttcaaca tccaaactcc
     3781 agacaccgat tgtattgtcc ttatgattat aagagtagct attgcctctt ttcatctcaa
     3841 aagtcttcca aacgggtgca tcattagcag ctgattcata aacgacaact tgcacttctg
     3901 tcgctgtagg tgaccagaga gaaaaatgag cctgattgtc ctctacacgg caacccaatt
     3961 ctccttggta accccaatga tgatcaaaac tagcactgtt aatggcctta tcaaaggcaa
     4021 aaggattttg atttttatag aaaggactgg caatagcagg attttcagag taataaatcc
     4081 tatcatcgcc ttccaaaatc cagacctctg ttaatagggg atagtgatta aaacggatag
     4141 aatattcttt actagtttga cctgtatgaa ccacaaaatt caagctttct ataacatgtg
     4201 aacttgggtg ttcaaagcta aataaagctc caaaataatc ttctttgtag gttagcaaat
     4261 caattcgttg atcctgactt tttacaaagg agcaagtgtc atattctcca ttcttacgat
     4321 ggtaatgaat gcgcataggg tagttataca ttttttattt tttatttttc ctttttactt
     4381 tgtttctatt tcactaataa atttttgtca atctcgtctc aattaacaga catagtcata
     4441 ttctctaaac tctgttttta aacgatccat tacaaacttt ctagccatgc ctcatctctg
     4501 acctggatac caagttcttg tgctttttgc agtttacttc cagcgtctgc acctaccacg
     4561 acgaggtcgg tctttttaga aacactacct gtcactttgg cacccagact ttcgagttta
     4621 cttttagctt ctgagcgctt gagtcgttcc aattttcctg tcaataccac ggtcaaacct
     4681 gacaaggccg catccgctac taccgtctgt cctttatagt ccagattgac cccagtttct
     4741 ttcaattctc tgagcagaat ttcagagcct tctgtcgcaa aataagtctg aagacttttg
     4801 gcaatcacgc cacctagact ttcaatacta gccacttcct ctgaatctgc ctgatacaga
     4861 ttttcaattg aatggaaata ttgaagtaaa agctgactag ccttgcttcc gacatgacga
     4921 attcccaaac caaataagag cttctcggca gaattttcct ttgatgcttg gatagcctga
     4981 tacagtttag cagcggactt ttccttaacc ccctctaaaa ggaggaaatc ctcttcttgc
     5041 aaacgataaa tatccgccac atccttgact aaattagcag caaaaagctt ctcaacaata
     5101 gatggaccaa ggcctgtaat attcatagca tcacgagaag caaagtgaat caagccttcc
     5161 atgatttgag cagggcaacg cggattgata caacgtaggg ccacttcatc ttcaaagtgc
     5221 aacaagtcag agttacaact tggacagttt gtagggatat ctagtttttc ttcagaaacc
     5281 cgtttggact ctaccacacg taaaacggca gggatgatgt caccagcctt atatacaatg
     5341 accgtatcgt cttttcggat atctttttca gcaatataat ctacattgtg cagggtcgca
     5401 cggctaacag tcgtaccggc aagttgtact ggtgttagat tagcagttgg agttacaaca
     5461 ccggtacggc caactgtcca gtcaactgat aagagttgag cttctttttc ttcagcaggg
     5521 aacttgtagg ctactgccca ctttggagcc ttaacggtaa aaccaagttc ttcttgactt
     5581 gctaggtcgt tgaccttgat taccactcca tcaatatcgt aaggcagatt ttcccgttcc
     5641 tgtcctactt cttggataaa attccagatt tcatctatgt tttcagccaa gattcgctta
     5701 ggattgacca caaaacctag ttgttctagg tacttcaaac ccttttcttg gctatcacga
     5761 gttgaagggc tggcttcttg atagagaaac gttgcaagat tacgcttggc aactactgct
     5821 gtatccaact gacgcagagt tcctgctgcc gcattacgag gattagcaaa ttcaggctct
     5881 ccattttctt ggcgcacttg gttaacttgg tcaaaggaag cgcgtggcat gtaacattcc
     5941 ccacgaactg tgatatctag ttcttctggc aaagtcaaag ggatgtcctt aacacgcttg
     6001 aggttttctg tgatattttc accaattgaa ccatctccac gtgttacccc agcaaccaaa
     6061 atcccctttt cataagtcag cgagatagat aagccatcga ttttcagctc acaaatatag
     6121 gtcggatgag ccacttcctt acgaacacgc gcatcaaaag catctagctc ctcacgtgaa
     6181 aaagcatcct gcaaactata aagaggatac tgatgactgt atttttcaaa accatctaaa
     6241 accttgccac caacacgatg agtcggactg tctgctagca cttgctctgg ataagcagtt
     6301 tctaactcga ccaactcacg gtaaaggcgg tcatactcac tgtctgaaac cgagggatta
     6361 tcgctggtat agtactcagt cgcatagcga ttgagcaaag cgactaactc attcattctt
     6421 ttattcataa gaccatttta ccataaaaca agccctcctc acaaacgaga aggacggaaa
     6481 aaacacttag tttgaaatta tttttgaaac tcaagcaacc ttatatcaat ttttcaaaat
     6541 gagttcgaac ataaataaac gatatacaag acaagatgat aacaccactt ccaattatca
     6601 ggaaagaaga gagatgtaca cttggcaaga ctgtcataaa tccttttgca ataggcataa
     6661 atagaatagc taaggtaaaa attgtactca gtactctccc aagaaattcg ctctcaacct
     6721 tggtttgtac ttgagtaaaa aagtgaatat taaaaatcgt cataaacaat tcacaaacta
     6781 aatttccaga aaaggaaaga aaagttggaa gtggtaatcc catcataaaa actccgacac
     6841 ctgtcaaagc cagtaaaatc aaaagattat aaatattagc tttaatttta ctagctagaa
     6901 gagccccaat gatggaacca atagccccca tagttaaaat acttgcatag gctccttctg
     6961 acccgtaaag ctgattcgaa aagggaagta gaaattcaaa agctgcaaaa aagaaattaa
     7021 cgctggaagc taccagcaaa aggaagaaaa tttcttgctg atgccagata tagtgtaacc
     7081 catccttgat atctacaaaa atatctctcc cagtaaaagc ctttttctct tgaacttttg
     7141 cttcctcttt tggaaggaaa gccactagaa caaaagcaat gaaaaaagtc agcgagtcta
     7201 gcagtagcgt catatggaga cttgcaaact gtaaaacaag gaaggaaaga acaggagagc
     7261 taacacctac aacctgcaaa accagctcta ggcgagaatt atagatcaca atctcatctt
     7321 tctccaccac ttcagttatg atagctttat tggctgtgcg agaaaaggca aaagcaatag
     7381 cctgcacaat gttagcaaca atcaaagcgc caatcatcca gctatcattc cttatgaaag
     7441 aaatagccag acaaagaatc ccacaaacaa gatctgccgt cattaaaatc ttacgacgag
     7501 aaaaacggtc tgaaataact ccgccaaagg gattgacgag aatagatgtg acgagctcag
     7561 aaatctgata cattcctaaa actgtctgtc ctatagtccc catggaagcc aaccagacac
     7621 tatttccata atcatagagc atatttccca ttttattgat agccccacgg ctaatcaact
     7681 gcactgcata gcgattcata ttaaagctcc tctcaaattt tgaaactatt gtatcaaaac
     7741 cgaaaggagc tttttatttt ttcccttatt tgggaaaatt aacttttgac aaatttttcg
     7801 tagtgttcct gataataggc tacttgctct ggaagaccta acacatcaaa aatatgcatg
     7861 gcctcttgca tctgcttaca gccttcttta cactgtcctt tttgatataa ggcaaaacct
     7921 tttaaataat ggaaaacatt acgctcataa agcttaatac ctttgtcaat aatcttctct
     7981 gtataagcct caaaatagtt ggcattataa aaagaagaat gatctaaaca atgctggtaa
     8041 caattgaggg ccaaaatcaa cactaatctc ttatggcgac taatctcttg gtaaaattcc
     8101 tccctctcca taacttctct accaatccga gtgacatagt ctacatcgta gaaactatag
     8161 aggttaccga aaagaatcaa ctcatacatg gtccattctt ctgttttgaa gagataatct
     8221 gctaccttat ccaaatcatc ctgcttcata tcataactcg catctctttg acaaatcaga
     8281 ccttgtagca aaatccagtt cagctcaaaa taaaggggag tcgtcgaact cttagacttt
     8341 tcaagttgtt ctctttgaag cttttgaaaa cctgcaatat cgtttgaata gtaaagtggg
     8401 ataatctgtg ccatcataga cacatgttca tgattatgaa aattccttgc cttatccatg
     8461 aaattttcga ttgttacatg aatgttatcc aaaatctcaa agaaacggga gactgccagg
     8521 tcagactccc caagctcaaa gcgagataac tgagaggtag agcaggattc gcctgctgct
     8581 tcctttaaag aataatttcc acttgttcga aattcacgaa atacttttcc aagatgttcc
     8641 atctttacac ctgatctgat aattcttccc actcaagcat agcttcttcc tgacgatggc
     8701 tgattttgtc cagctcagcc tgtaattcca tgagtttgtc ggcatcgttt gtttccaaca
     8761 tttgttcaga aatggcttgg ctttgacttt ctagctcttc aatttcagct tctagacttt
     8821 cgatttgtcg catgagtttg cgaacttctt tttgactttc tttctgggtc tgatagtcat
     8881 tgactggact tgcttccttt gcttgattgc tagttgaagc ttcctcagtc tgactcattt
     8941 ctgctgttgc tttcttctca acatagtagt cgtaatctcc aaggtagaga gttgaaccat
     9001 tctcagacaa ttccaaaaca tgagttgcca cacgattgat aaagtaacga tcatgactga
     9061 caaaaagcaa ggtaccatca aagtcaatca aggcattttc tagcacttcc ttactatcaa
     9121 tatccaagtg gttggtcggc tcatccagaa tcaaaaagtt attgttttcc atagacaatt
     9181 tagctaaaag caaacgagct ttttcgccac cagatagcat gccgactgat tttttaacat
     9241 catctcctga gaaaaggaag gctccaagac ggttgcggat ttcaacttct ggtgtcagtt
     9301 tgaaatcatt ccagagttca tccagcaccg tattacttgg tgtcagcttg ctttgggttt
     9361 ggtcatagta acccacctca acattagcgc caaagcgctt ttctcccttg ataaaaggaa
     9421 tctggtccac aatagacttg ataaaggttg acttgccgat accatttgga ccaacgatag
     9481 cgacagcatt catcttacga agatctaggt tgataggttg tgacaagact tccccgtcat
     9541 aaccaatagc agtattttca acagtcaaaa caacattgcc cgacgttttt tcagactgga
     9601 aggtcatgtt ggctgctttc ttgccagctt caggcttgtc caaacgctcc attttttcta
     9661 gttgtttacg gcgagattga gcacgtttag tcgttgaagc acgaactaga ttgcgattga
     9721 caaagtcttc cagagcagcg atttccttct gttgcttttc atagtttttt gcctcagtag
     9781 ctagcttttg ctccttcaat tcgacaaaac gagagtaatt ccccacatag cgatccaagg
     9841 aatgcttggt caaatctagc gtaattgtcg caaccttgtc caagaaataa cggtcgtggc
     9901 tgacgataag gagggcaccg ctatagttta ccaagtaatt ctctagccag gcgatggttt
     9961 caatatccaa gtggttagtt ggctcgtcca agaccaagag attgggcttt tcaaggagca
    10021 ttttggcaag tgccaaacga gtattttgac caccagaaag ctcagcaatt ttcatctgcc
    10081 acatagactc gtcaaacttg aatccattca aaatcgctcg aatatcagct tcataggtaa
    10141 agccacctgc ttggcgaaaa ttctcagata agcggtcata atctgacatc agtttatcca
    10201 aatcctcacc agacttttca cccatctcca gctccatctg acgcagttgt ctctccgtcc
    10261 gacgcaaatt attaaagaca tgaagcattt catcgtagat ggtattttca gactcaaaac
    10321 ggctatcttg ggctaggtaa gacagagaaa tatctttttt cttattgatt tctccgctag
    10381 ttggctcctc ttctccaact aaaatcttca aaagagtaga cttacctgca ccatttttcc
    10441 caacaagggc aatccgatct cgttcatcaa cctgcaggtt gatattatcg aaaagaacct
    10501 ctcctgcaaa agaacgttca attttattag cttgtaaaat aatcataaga gtagtatagc
    10561 atgtttccct aaggcattca agataatcgt aagtctttta gtacaacttt tataacataa
    10621 aataaactaa attatgtata ttttatatta gattcttcac tatcttgttg gattttctaa
    10681 ccagctaatc ttgtttcaaa tagttatcgc acaagcctat tatttaattc ttttcattat
    10741 ttacgtac
//
LOCUS       DAVHIT010000066        10694 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVHIT010000066 DAVHIT010000000
VERSION     DAVHIT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.067"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            97..594
                     /locus_tag="VVN16_001564"
     CDS             97..594
                     /locus_tag="VVN16_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5572910.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDTMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            594..2249
                     /gene="dnaX"
                     /locus_tag="VVN16_001565"
     CDS             594..2249
                     /gene="dnaX"
                     /locus_tag="VVN16_001565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU5572911.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTTISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTNLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2277..2471
                     /locus_tag="VVN16_001566"
     CDS             2277..2471
                     /locus_tag="VVN16_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU5572912.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2621..3391
                     /gene="sufC"
                     /locus_tag="VVN16_001567"
     CDS             2621..3391
                     /gene="sufC"
                     /locus_tag="VVN16_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU5572913.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3419..4681
                     /gene="sufD"
                     /locus_tag="VVN16_001568"
     CDS             3419..4681
                     /gene="sufD"
                     /locus_tag="VVN16_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU5572914.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4692..5918
                     /locus_tag="VVN16_001569"
     CDS             4692..5918
                     /locus_tag="VVN16_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5572915.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5905..6345
                     /locus_tag="VVN16_001570"
     CDS             5905..6345
                     /locus_tag="VVN16_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU5572916.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6399..7811
                     /gene="sufB"
                     /locus_tag="VVN16_001571"
     CDS             6399..7811
                     /gene="sufB"
                     /locus_tag="VVN16_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5572917.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8042..9283)
                     /locus_tag="VVN16_001572"
     CDS             complement(8042..9283)
                     /locus_tag="VVN16_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU5572918.1"
                     /translation="MKKTFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSKASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9386..10495
                     /locus_tag="VVN16_001573"
     CDS             9386..10495
                     /locus_tag="VVN16_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5572919.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3177 a         1952 c         2319 g         3246 t
ORIGIN      
        1 tttacccact acaaatatta tagagcccaa aatcctttgt ttactaaaca agggattttt
       61 cttttgtctc tgctcctttt ttgatataat agttctatgt taaaatcaga aaaacaatca
      121 cgttatcaaa tgttaaatga agaattgtcc ttcctattgg aaggcgaaac caatgttttg
      181 gctaatcttt ccaacgccag tgctctcata aaatcacgtt ttcctaatac cgtatttgca
      241 ggcttttatt tgttcgatgg aaaggaattg gttttaggcc ccttccaagg aggtgtttcc
      301 tgcatccgta ttgcactagg caagggtgtt tgtggtgagg cagctcactt tcaggaaact
      361 gttattgttg gagatgtgac gacctatctc aactatattt cttgtgatag tctagctaaa
      421 agtgaaattg tggtgccgat gatgaagaat ggtcagttac ttggagttct ggatctggat
      481 tcttcagaga ttgaggatta cgatactatg gatcgagatt atttggaaca atttgtcgct
      541 attttgcttg aaaagacagc atgggacttt acgatgtttg aggaaaaatc ttaatgtatc
      601 aagcacttta tcgaaaatat agaagtcaaa acttctccca gttagttggt caagaagttg
      661 tggctaagac tcttaaacaa gcggtggagc aagagaaaat aagtcacgct tatctttttt
      721 ctggtcctcg tggaacggga aaaaccagtg ttgctaaaat ctttgccaag gctatgaact
      781 gtcccaatca agtgggtggc gaaccttgca ataactgcta tatttgtcaa gcagtgacgg
      841 acggtagttt agaagatgtc attgaaatgg atgcagcttc taataatggg gtagatgaaa
      901 ttcgcgaaat tcgtgataaa tctacctatg cgcctagcct tgctcgttat aaggtttata
      961 tcatagatga ggttcacatg ctgtctacag gagcttttaa tgccctccta aagacgctgg
     1021 aagaaccaac acagaatgta gtctttattt tggccactac tgaattgcac aagattcctg
     1081 ctactattct atcccgtgtg caacgttttg agtttaaatc aattaagaca caggatatta
     1141 aggaacatat tcactatatc ttagaaaaag aaaatatcag ttctgaacca gaggctgtgg
     1201 aaatcattgc cagacgggcg gaaggtggaa tgcgggacgc cttgtctatt ttggatcaag
     1261 ccctgagttt gacacaggga aatgagctga cgactactat ctctgaagaa attactggca
     1321 ccattagcct atcagccttg gatgattatg tggcggcctt gtctcaacag gatgttccca
     1381 aagctttgtc ttgcttgaat cttctttttg acaatggtaa gagcatgact cgttttgtga
     1441 ccaatctttt gcactattta agagacttgt taattgttca aacaggggga gaaaatactc
     1501 atcatagttc agtctttgta gaaaatttgg cacttcctca aaaaaatctg tttgaaatga
     1561 ttcgcttagc aacagtgagt ttagcagata ttaagtctag tttgcagccc aagatttatg
     1621 ctgaaatgat gaccgtccgt ttggcggaaa tcaagtccga accagctcta tcaggagcgg
     1681 ttgaaaatga aattgctacg ctgagacagg aagttgcccg tctcaaacaa gagctttcta
     1741 atgtaggtgc ggttcctaaa caagttgcac cagctcctag tcgaccagct acgggcaaaa
     1801 cagtctatcg tgtcgatcgc aataaagtgc aatctatctt acaagaggcc gtcgaaaatc
     1861 ctgatttagc acgtcaaaat ttaattcgtt tgcagaatgc ctggggagag gtaattgaaa
     1921 gtctaggtgg gccggacaag gctctgctag ttggttctca accggttgct gccaatgaac
     1981 accatgctat tcttgctttt gagtctaact tcaatgctgg tcaaactatg aaacgagaca
     2041 atctcaatac catgtttggt aatatcctca gtcaggcggc aggtttttca cctgagattt
     2101 tagctatttc catggaggaa tggaaagaag ttcgcgcagc cttttcagcc aaagccaaat
     2161 cttctcaaac tgaaaaagaa gtagaagaaa gcctgattcc agaaggattt gaatttttgg
     2221 ctgataaagt gaaggtagag gaagactaaa gaaagatttc atgatacaat aagtttatga
     2281 ataaacaaca atttattatt atggcgctgt ttacagctgc tgagacctat tttttcaatg
     2341 aagcctggat gactggccgc tatattatgg cagccttttg ggcaatttta ctatttagaa
     2401 atttccgagt cagttatgtg atgggcaaaa tcgttgatgt catcgatcag cattttaata
     2461 ggaaagacta gccctcagct tccagacaaa atcaaagcct tttaggcttt tttttgttat
     2521 actagaaaag tatatttata gaatttttgc tctatttctg gggaaatcag acgtttttct
     2581 agtaagtact gtaaaagttt tgaaaaagaa aggaactatc atgtcagtat tagagatcaa
     2641 agatcttcac gttgagattg aaggaaaaga aattttaaaa ggggttaacc tgaccctgaa
     2701 aacaggagaa attgccgcta tcatgggacc aaatggtaca ggtaaatcga ctctttctgc
     2761 cgctatcatg ggaaatccaa actatgaagt aactaaaggt gaagttttgt ttgatggcgt
     2821 aaacatcctt gagttggaag tggatgagcg tgcgcgtatg ggacttttcc ttgctatgca
     2881 atacccatca gaaatccctg gaattaccaa tgctgagttt cttcgtgccg ctatgaatgc
     2941 gggtaaagaa gatgatgaga agatttcagt tcgtgagttt attactaagc tagatgaaaa
     3001 aatggaattg ctcaacatga aagaagaaat ggcagagcgt tacctcaacg aaggcttctc
     3061 tggcggtgag aaaaaacgca atgaaattct tcaacttttg atgttggagc caacatttgc
     3121 tcttttggat gagattgact caggtcttga tattgacgct cttaaagttg tgtctaaagg
     3181 tgtcaatgcc atgcgtggtg aaggttttgg tgctatgatc attactcact accaacgtct
     3241 tttgaactat atcacacctg atgtggtaca cgtgatgatg gaaggtcgtg ttgtcctttc
     3301 tggtggtcca gaattggctg cgcgtttgga acgtgaagga tacgcaaaat tagctgaaga
     3361 acttggctac gactacaagg aagaattgta atttcctcgt atcttttagg agaagtaaat
     3421 gactagagaa aatattaaac ttttttcaga aatgcacgct gaaccaagct ggttggctga
     3481 tctccgtcaa aaagcttttg acaagattga gactttggaa ttaccagtta ttgagcgtgt
     3541 caaattccac cgttggaatc tgggtgatgg aacgattaca gaaaatgagc catcagcaaa
     3601 tgttccagat ttcacagctt tagatcatca cttgaagttg gtgcaagtag gaactcaaac
     3661 tgttttcgaa caaactccag ttgagttagc tgaacagggt gttgtcttca cagactttca
     3721 ctcagcttta gaagaaattc cagagctgat cgaagaattc ttcatgtcat ctgttaagta
     3781 tgatgatgac aagttggcgg cttaccacac agcttacttt aacagtggtg ctgtactcta
     3841 tattccagat aacgtagaaa ttacagagcc aattgaagga attttctacc aagatagcga
     3901 tagcaatgtg ccgtttaaca agcatattat gattatcgct ggtaaaaatt ctaagattag
     3961 ttatctggag cgtttagagt cacgcggtga aggaagtgac aaagtaactg ccaatatcac
     4021 agtggaagtg attgcacgtt ctggtgcgca agtcaagttt gctgctatcg accgtctagg
     4081 tgaaaacgtc actgcctaca ttagccgtcg tggtaaatta ggcaacgatg caagtattga
     4141 ctgggctatc ggtgtcatga acgaaggaaa tgtcgttgct gattttgata gtgacttgat
     4201 tggtaatggt agccatgctg acctcaaggt tgtagctctt tcaagtggtc gtcaggtaca
     4261 agggattgat actcgtgtaa ctaactatgg ctgcaactca atcggaaaca ttctacaaca
     4321 tggggttatc cttgaaaaag caactttgac tttcaatggt atcggccaca tcatcaaggg
     4381 tgctaaggga gcagatgcgc aacaagagag ccgtgttctc atgctttcag accaagcgcg
     4441 ttcagatgct aacccaattc ttttgattga tgaaaatgac gtaactgcag gacatgcagc
     4501 ctctattggt caggtagatc cagaagacat gtattacctc atgagtcgtg gattggataa
     4561 ggcaactgca gagcgtttgg ttgttcgtgg tttccttgga tctgttatcg tggagattcc
     4621 agtcaaggaa gttcgtgatg aaatgattgc aactatcgaa gagaaattgt caaaacgcta
     4681 aggggaagcc tatgttagat gtagaagcga ttcgtaagga ttttccaatt ttagatcaga
     4741 ttgtcaatga tgaacctctg gtctatctgg acaatgctgc gacgacacaa aaaccactag
     4801 tagttctgaa agctattaac agctactatg aacaggacaa tgccaatgtt caccgtggtg
     4861 tccatacctt agcggaacga gcgacagctt cttatgaagc tgctcgtgaa accattcgta
     4921 agtttattaa tgcaggctct acaaaggaag ttctctttac cagaggaacg acaaccagcc
     4981 ttaactgggt ggcacgcttt gctgaggaaa ttctcactga gggagaccag gtcttgattt
     5041 cagtaatgga acaccattct aatatcattc catggcagga agcttgtcga aagactggag
     5101 cagagcttgt ctatgtctat cttaaagacg gtgccttgga tatggaggat ttgcgagcta
     5161 aattgactga taaggttaaa tttgtttccc tagctcatgc ctccaatgtt cttggtgtgg
     5221 tcaatccgat caaggaaatc actcaattag cccaccaagt tggggcaatc atggtagtgg
     5281 atggtgctca atctacacct catatgaaga ttgatgtcca ggacttggat ctggactttt
     5341 tcgccttttc gggtcacaag atggctggtc cgactggtat cggtgtcctt tacggcaaag
     5401 aaaagtatct tgagcaaatg tctccagtag aatttggcgg cgagatgatt gattttgtct
     5461 acgagcaatc tgctagttgg aaggaattgc cttggaaatt tgaggctgga acgccaaata
     5521 tggcaggagc tattggactt gcgactgcag ttgattatct ggaaaagatt ggtatggatg
     5581 ccgttgaagc tcatgaacag gaattgattg cgtacgtcta tccaaaactg caggcaattg
     5641 agggattgac catttacggt tctcaggatt tggctcaacg ttcgggtgtt attgccttta
     5701 acctaggtga tctccatcct cacgatcttg cgacggctct ggattatgaa ggagtggctg
     5761 ttcgtgctgg tcaccattgt gcgcaaccct tgcttcagta tttggaagtc ccagcaacag
     5821 ctcgtgcaag tttttatatc tacaatacca aggcagattg cgacaaacta gtcgatgccc
     5881 tacaaaagac aaaggagttt ttcaatggca ctttctaaac tagatagcct ttatatggca
     5941 gtggtagcag accattcgaa aaatccacat caccaaggga agttagaaga cgctgagcaa
     6001 atcagtctca acaatccgac ttgtggggat gtcatcaacc tctctgtcaa gtttgatgca
     6061 gaggaccgtt tggaagatat tgcttttcta aattcaggat gcacgatttc aactgcttct
     6121 gctagtatga tgacagatgc cgttttagga aaaaccaaac aagaaatttt agaactggcg
     6181 actatttttt ctgaaatggt tcaagggcaa aaagatgagc gtcaagacca acttggagat
     6241 gcggcattct tgtcaggtgt tgccaaattc cctcaaagaa tcaagtgtgc aaccctagct
     6301 tggaatgccc ttaagaaaac aattgaaaat caagaaaaac agtaagacaa gtttcttttg
     6361 tcttatgaat tattagaaat gaagaaagaa aggatattat ggctgaagaa agagtagaac
     6421 caaaaccaat tgaccttggt gaatataaat ttggtttcca tgacgatgta gagcctgtct
     6481 tatcgacagg aaaaggactc aacgaaggtg ttattcgtga attatctgct gctaagggtg
     6541 agcctgagtg gatgttggag ttccgtttga agtcttatga aaccttcaaa aaaatgccca
     6601 tgcaaacttg gggagcagac ttgtcagaga ttgactttga tgacttaatc tactaccaaa
     6661 aaccatctga caaaccagcc cgttcttggg atgatgtacc tgaaaagatt aaagaaacct
     6721 ttgaacgtat cgggattcca gaagctgaac gtgcttattt agcaggggct tctgcccagt
     6781 acgagtcaga agtggtttac cacaacatga aggaagagtt ccaaaaatta ggtattatct
     6841 ttacagatac agattccgca ctcaaggaat acccagactt atttaaacaa tactttgcga
     6901 agttggtacc gccgacagat aacaagttgg cagccctcaa ctcagcagta tggtcgggtg
     6961 gaacttttat ctacgtgcca aaaggtgtca aggtagatat tccacttcaa acttatttcc
     7021 gtatcaataa cgaaaatata ggtcagttcg aacgtacctt gattatcgtt gatgagggag
     7081 caagcgtcca ctacgtagaa ggatgtacag caccaacata ttcaagcaat agcttgcacg
     7141 ctgccattgt agaaattttt gctttggacg gagcttatat gcgttataca actatccaaa
     7201 actggtctga taacgtctat aacttggtaa caaagcgtgc taaggctcaa aaggatgcca
     7261 ctgttgagtg gattgatgga aacttgggtg ccaaaacgac tatgaaatat ccatctgttt
     7321 accttgatgg agaaggagcg cgtggtacca tgctctctat cgcctttgct aatgcagggc
     7381 aacaccaaga cacgggtgct aagatgattc acaatgctcc acataccagc tcgtctattg
     7441 tgtctaaatc catcgctaaa ggtggaggaa aggttgacta ccgtggacaa gtcaccttta
     7501 acaagaactc taagaaatct gtttcccaca ttgaatgtga taccattatc atggatgact
     7561 tgtcagcatc agatactatt ccatttaatg aaattcacaa ctcgcaagta gctttggaac
     7621 acgaagccaa agtatctaag atttcagaag agcaattgta ttatctcatg agccgtggat
     7681 tgtcagaatc tgaggcaact gaaatgattg tcatgggatt tgtagaaccc tttacaaaag
     7741 aacttccaat ggaatacgca gttgagctga accgcttgat tagctatgaa atggagggat
     7801 cagttggata aaatttgatt ttatactctt cgaaaatctc ttcaaaccac gtcagcatcg
     7861 ccttaccgta tgtatggtta ctgacttcgt cagtttcatc tacaacctca aaacagtgtt
     7921 ttgagcaacc tgcggctagc ttcctagttt gttctttgat tttgagtatt agatttactc
     7981 aaaatcaagg attttgaaga tgaacttgta tcaaaaaatc gcggtttaaa atcgcgattt
     8041 tttataattt ctcgttaaca aagcggacaa actgattcca ccaaactttt aagaagaagg
     8101 ctttttcaat tttcttgtct gctaccattt cgaaactagg gcgctctgtg gtgatgtaac
     8161 cttgaccaat caagtccttg tcttcataag tcaaatggcc aaccactgtt ccagcttcaa
     8221 gtggtgctgg gattgctttg gaatcaggtg tgaattgaac agattgggaa gattgattcc
     8281 caacacgttc tattagatag atatcctctg gagccactgc agttactgta tcttcttttc
     8341 catcttgtac aggggctttg ctatcttgat aggcatcgcc ttgttgaacg attttgcgaa
     8401 gtgtaaatgt agaagaaata taatccatta gggaagatgt agctgtaaat cgagcataag
     8461 gattattgtc ttgatgatct gcatttaaaa caactgtgat gactctcatg cctttttcga
     8521 cagtagtacc aacaaaagac tctccagcct tatctgttgt tcctgttttt agcccatcaa
     8581 aaccaccacg gtaagcaggc ataccttcta acatgtagtt agttgaagtg attgtcatcc
     8641 cagcaaaagt agaagaaggt ttttttgtga tttctaagac ttgtgggtat tttttgatga
     8701 ggttgcgagc aacgatagcg acatcataag cactaagctt attttcctca tcttttttag
     8761 aacctgggta aatgttatcc cctagagttt cattgttaag acctgtcgta ttgacaacag
     8821 tggcatcctg aattccccat tccaagagtt ttgcccgcat catatcgacg aaatcttttt
     8881 ctgagccagc aattttctca gctagggcaa tagcggcgct gttggcacta gataccagag
     8941 ttgcttcaag caactcttcg acagtataat tacgggcctc cataggaata ttactggctt
     9001 tagaatttgt cgtcaattga taaggataat cagaaatatc tacaggagtg gagagggtaa
     9061 tacttccgtt ttccaaagct tcatagacca gataaacagt aatcaatttt gttatggaag
     9121 caatttcgac aggttgcgtt gcatccttct catagagaat tttaccagta tttgcctcaa
     9181 cagcaatcgc atgtttagcg gcaatggtaa aatcttgagc aacagcagta gaagcacccc
     9241 ctaaaagaga gacagttaat aaagttaaaa atgttttttt catagtagtc ttattctatc
     9301 ataaagaaaa aaaatattct tgctttaata attcatctgt taagcttttt gaaaatatgg
     9361 taaaataaag tgagggaggt aactcatgtt tcgtagaaat aaattatttt tttggaccac
     9421 agaaatttta ctcttaacca tcatctttta cctatggaga cagatgggat ctttgattaa
     9481 cccttttgtt agcgtgctta atacaattat gattccattt ttattagggg gctttcttta
     9541 ttatttgaca aaccctattg ttactttctt aaataaagtc tgtaaactca atcgtttgct
     9601 tggtatttta attaccttgt gtactttggt ctggggaatg gtcataggtg ttgtctatct
     9661 cttacctatt ttgattaatc agttatctag tttgattata tctagtcaaa ctatttatag
     9721 tcgagtacaa gacttaatca tagacttatc taattatcct gcgctccaga atttggatgt
     9781 agaagctaca attcagcagt taaacttatc ctatgttgat attcttcaaa atatcctaaa
     9841 tagcgtatca aatagtgtgg ggagcgtctt gtcagctctt atcagtactg ttttgatttt
     9901 gattatgact ccagtttttt tggtttattt cttattagat ggacataaat tcttgcccat
     9961 gcttgaaaga acgattctaa agagggatcg cttgcatatt gcaggcttat taaagaattt
    10021 aaatgcgacg attgctcgct atattagtgg agtttcgatt gacgcaatca ttataggttg
    10081 tttagcttat attggctata gtattattgg tttaaaatat gctttagttt ttgccatttt
    10141 ttctggtgta gccaatttaa ttccttatgt ggggccaagt attggtttga ttcctatgat
    10201 catcgcaaat atattcactg atccccatag actgctgatt gcagtgattt atatgcttgt
    10261 tgttcagcag gtagatggca atatcttata tcctcgaatc gtaggaagtg ttatgaaggt
    10321 tcatccaatc acgattttag ttttactttt gttgtcaagc aatatctatg gtgtagttgg
    10381 aatgattgtc gcagtgccaa cctattctat cttgaaagaa atttctaagt tcttatcccg
    10441 tttgtatgaa aatcataaaa taatgaaaga acgagaaaga gaattagcta agtaaaagtc
    10501 aggagaaccc tgatttttct ttactggaag tggcctttag attagaagac tgaaaataag
    10561 ttaaagtctt aaactaattt tcacagctaa gaatagtaga agttaatctg ataaaaatcg
    10621 aaaaaaccag tggaattccg tgtcagggta agttccactg gttttcatag tctattaaag
    10681 ttcgaatgaa acct
//
LOCUS       DAVHIT010000067        10550 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVHIT010000067 DAVHIT010000000
VERSION     DAVHIT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.068"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1072
                     /gene="dnaN"
                     /locus_tag="VVN16_001574"
     CDS             <1..1072
                     /gene="dnaN"
                     /locus_tag="VVN16_001574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5572920.1"
                     /translation="SSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDAG
                     LLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYP
                     RIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDS
                     HRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIEAVEIFFANNQILFRSENISF
                     YTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGV
                     VSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRP
                     FTLVPADTDEDFMQLITPVRTN"
     gene            1137..1331
                     /locus_tag="VVN16_001575"
     CDS             1137..1331
                     /locus_tag="VVN16_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU5572921.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1415..2530
                     /gene="ychF"
                     /locus_tag="VVN16_001576"
     CDS             1415..2530
                     /gene="ychF"
                     /locus_tag="VVN16_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5572922.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2601..3170
                     /locus_tag="VVN16_001577"
     CDS             2601..3170
                     /locus_tag="VVN16_001577"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5572923.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            3171..6680
                     /gene="mfd"
                     /locus_tag="VVN16_001578"
     CDS             3171..6680
                     /gene="mfd"
                     /locus_tag="VVN16_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5572924.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNNHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6738..7004
                     /locus_tag="VVN16_001579"
     CDS             6738..7004
                     /locus_tag="VVN16_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU5572925.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            6997..7365
                     /locus_tag="VVN16_001580"
     CDS             6997..7365
                     /locus_tag="VVN16_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5572926.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7485..8753
                     /locus_tag="VVN16_001581"
     CDS             7485..8753
                     /locus_tag="VVN16_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU5572927.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8750..10027
                     /gene="tilS"
                     /locus_tag="VVN16_001582"
     CDS             8750..10027
                     /gene="tilS"
                     /locus_tag="VVN16_001582"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5572928.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPKLEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKTQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10031..>10550
                     /gene="hpt"
                     /locus_tag="VVN16_001583"
     CDS             10031..>10550
                     /gene="hpt"
                     /locus_tag="VVN16_001583"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5572929.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVL"
BASE COUNT         3659 a         1564 c         2107 g         3220 t
ORIGIN      
        1 tagttctaaa aatgccattc ctattttatc aacagtaaaa attgacgtga ccaatgaagg
       61 tattacttta attggttcaa atggtcaaat ttcaattgaa aattttattt ctcaaaaaaa
      121 tgaagatgct ggtttgttaa ttacttcttt aggttcgatc cttcttgaag cttctttctt
      181 tatcaatgta gtatctagtt tacctgatgt aactcttgat tttaaagaaa ttgaacaaaa
      241 tcaaattgtt ttaaccagtg gcaaatcaga aattacccta aaaggaaaag atagcgaaca
      301 atatccacga atccaagaaa tttcagcaag cactccttta atacttgaaa caaaattact
      361 caagaaaatt attaatgaaa cagcctttgc tgcaagtaca caagagagtc gtccgatttt
      421 aacaggtgtc cacttcgtat tgagtcaaca caaagagtta aaaacagttg caacagactc
      481 tcatcgccta agccagaaaa aattgactct tgaaaaaaat agtgatgatt ttgatgtcgt
      541 aattcctagc cgttctctac gcgaattttc agcggtattt acagatgata tcgaagctgt
      601 agagattttc tttgccaata accaaatcct ctttagaagc gaaaatatta gcttctatac
      661 tcgtctccta gaaggaaact atcctgatac agatcgcttg attccaacag actttaacac
      721 tactattact tttaatgtgg taaacttacg ccagtcaatg gagcgtgccc gtcttttatc
      781 aagtgcgact caaaatggta ctgtgaaact tgaaattaag gatggggttg ttagcgccca
      841 tgttcactct ccagaagttg gtaaagtaaa cgaagaaatc gatactgatc aggttactgg
      901 tgaagatttg accattagtt tcaacccaac ttacttgatt gattctctta aagctttaaa
      961 tagcgaaaag gtgaccatta gctttatctc agctgttcgt ccatttactc ttgtgccagc
     1021 agatactgac gaagacttca tgcagctcat tacaccagtt cgtacaaatt aagtgaaaga
     1081 ggttgagcct ggctcgcctc ttttatgata taatcgaaaa agaaaaggag agtagtatgt
     1141 atcaagttgg aaattttgtt gagatgaaaa aatcacacgc ttgtacaatc aagtcgactg
     1201 gtaaaaaggc taatcgttgg gaaattacac gtgtaggagc agatatcaaa ataaaatgca
     1261 gtaattgtga gcatgttgtc atgatggggc gatatgattt tgagcgaaaa atgaataaaa
     1321 ttattgactg agaaccctta gttagagggt tagcacttta tccctttttg tgttataata
     1381 ttagggattg aaatgaaaac ggagaatgag aaatatggct ttgacagcag gtatcgttgg
     1441 tttgccaaac gttggtaaat caacactatt taatgcaatt acaaaagcag gagcagaggc
     1501 agcaaactac ccatttgcga ctattgatcc aaatgttgga atggtggaag ttccagatga
     1561 acgcctacaa aaactaactg aaatgataac tcctaaaaag acagttccca caacatttga
     1621 atttacagat attgcaggga ttgtaaaagg agcttcaaaa ggagaagggc tagggaataa
     1681 attcttggcc aatattcgtg aagtagatgc gattgttcac gtagttcgtg cttttgatga
     1741 tgaaaatgta atgcgcgagc aaggacgtga agacgccttt gtggatccac ttgcagatat
     1801 tgacaccatt aacctagaat tgattcttgc agacttagaa tcagtcaata aacgatatgc
     1861 gcgtgtagaa aagatggcac gtacgcaaaa agataaagaa tcagtagcag aattcaatgt
     1921 tcttcaaaag attaaaccag tcctagaaga cgggaaatca gctcgtacca ttgaatttac
     1981 agatgaggaa caaaaggttg tcaaaggtct tttccttttg acgactaaac cagttcttta
     2041 tgtagctaat gtggacgagg atgtggtttc agaacctgac tctatcgact atgtcaaaca
     2101 aattcgtgaa tttgcagcga cagaaaatgc tgaagtagtc gttatttctg cgcgtgctga
     2161 ggaagaaatt tctgaattgg atgatgaaga taaaaaagag tttcttgaag ccattggttt
     2221 gatagaatca ggtgtagata agttgacgcg tgcagcttac cacttgcttg gattgggaac
     2281 ttacttcaca gctggtgaaa aagaagttcg cgcttggact ttcaaacgtg gtatgaaggc
     2341 tcctcaagca gctggtatta tccactcaga ctttgaaaaa ggctttattc gtgcagtaac
     2401 catgtcatat gaagatctag tgaaatacga atctgaaaag gccgtaaaag aagctggacg
     2461 cttgcgtgaa gaaggaaaag aatatatcgt tcaagatggc gatatcatgg aattccgctt
     2521 taatgtctaa aaattaataa atggtgtcaa ttaggttgga aaaaaattcc aacccttttg
     2581 gcttttgaaa ggaaaaataa atgaccaaat tacttgtagg cttgggaaat ccaggggata
     2641 aatattttga aacaaaacac aatgttggtt ttatgttgat tgatcaacta gcgaagaaac
     2701 agaatgtcac ttttacacac gataagatat ttcaagctga cctagcatcc tttttcctaa
     2761 atggagaaaa aatttatctg gttaaaccaa cgacctttat gaatgaaagt ggaaaagcag
     2821 ttcatgcttt attaacttac tatggtttgg atattgacga tttacttatc atttacgatg
     2881 atcttgacat ggaagttggg aaaattcgtt taagagcaaa aggctcagca ggtggtcata
     2941 atggtatcaa gtctattatt caacatatag gaactcaggt ctttaaccgt gttaagattg
     3001 gaattggaag acctaaaaat ggtatgtcag ttgttcatca tgttttgagt aagtttgaca
     3061 gggatgatta tatcggtatt ttacagtctg ttgacaaagt tgacgattct gtaaaatact
     3121 atttacaaga gaaaaatttt gagaaaacaa tgcagaggta taacggataa atggtgacct
     3181 tattagattt attctcagaa aatgatcaga ttaaaaaatg gcatcaaaat ttaacagata
     3241 agaaaagaca actaatactt ggtttatcaa catctactaa ggctcttgca attgcaagca
     3301 gtttagaaaa agaagatagg attgtgttat tgacgtcaac ttatggagaa gcagaaggac
     3361 ttgttagtga tcttatttct atcttgggtg aggaactcgt ctatccattt ttggtagatg
     3421 atgctcctat ggtggagttt ttgatgtctt cacaggaaaa aattatttca cgggttgaag
     3481 ccttgcgttt tttgactgat tcatctaaga aagggatttt agtttgtaat atcgcagcaa
     3541 gtcgattgat tttaccgtct cccaatgcat tcaaagatag tattgtaaaa atctcagttg
     3601 gtgaagaata tgatcaacac gcgtttatcc atcagttaaa ggaaaatggc tatcgaaaag
     3661 ttactcaagt acaaactcag ggcgaattta gtcttcgagg agatatttta gatatttttg
     3721 aaatatccca gttagaacct tgtcgaattg agttttttgg tgatgaaatt gatggtatca
     3781 ggtcatttga agtagaaaca caattatcga aagaaaataa gacagaactc actatctttc
     3841 cagctagtga tatgcttttg agagaaaagg attatcaacg aggacagtca gctttagaaa
     3901 aacaaatttc aaaaacttta tcacctattt tgaaatcata cctagaagaa attctttcaa
     3961 gttttcacca aaaacaaagt catgcagact ctcggaagtt tttatctttg tgctatgata
     4021 agacatggac tgtctttgat tatattgaaa aagatactcc aatattcttt gatgattatc
     4081 aaaaattgat gaatcagtat gaagtctttg aaagagaatt agcgcagtac tttacagaag
     4141 aattacagaa tagtaaagca ttttctgata tgcagtattt ttctgatatt gaacaaatct
     4201 ataaaaaaca aagtccagtg acctttttct ctaatcttca aaagggttta ggaaatctca
     4261 aatttgacaa aatttatcaa ttcaatcaat atcctatgca ggaatttttc aatcagtttt
     4321 cttttctaaa agaagaaatt gaacgatata aaaaaatgga ttacaccatt attctgcagt
     4381 ctagcaattc aatgggaagt aaaacattgg aggatatgtt agaggaatat cagattaaat
     4441 tggattctag agataagaca agtatctgta aagaatctgt aaacttaata gagggtaatc
     4501 tcagacatgg ttttcatttt gtagatgaaa agattttatt gataactgaa catgagattt
     4561 ttcaaaagaa attaaagcgt cgttttcgaa gacaacatgt ttcaaatgca gagagattaa
     4621 aagattacaa tgaacttgaa aaaggggact atgttgtcca tcatatccat gggattggtc
     4681 aatatctagg aattgaaacc attgaaatca agggaattca tcgcgattat gtcagtgtcc
     4741 aataccaaaa tggtgatcaa atttctatcc ccgtggaaca gattcatcta ctgtccaaat
     4801 atatttcaag tgatggtaaa gctccaaaac tcaataaatt aaatgacggt cattttaaaa
     4861 aggccaagca aaaggttaag aaccaggtag aggatatagc tgatgattta atcaaactct
     4921 actctgaacg tagtcagttg aagggttttg ctttctcagc tgatgatgat gatcaagatg
     4981 cctttgatga tgctttccct tatgttgaaa cggatgatca acttcgtagt attgaggaaa
     5041 tcaagaggga tatgcaggct tctcagccaa tggatcgact tttagttggg gatgttggtt
     5101 ttggaaagac tgaagttgct atgcgtgcag cctttaaagc agtcaataat cacaaacagg
     5161 ttgtcattct agttccgacg acggttttag cgcaacagca ctatacgaat tttaaggaac
     5221 gattccaaaa ttttgcagtt aatgttgatg tgttgagtcg ctttagaagt aaaaaagagc
     5281 agactgcaac acttgaaaaa ttgaaaaacg gtcaagtcga tattttgatt ggaacacatc
     5341 gtgttttgtc aaaagatgtt gtgtttgctg atttgggctt gatgattatt gatgaggaac
     5401 agcgatttgg tgtcaagcat aaggaaactt tgaaagaact gaagaaacaa gtggatgtcc
     5461 taaccttgac cgctacgcca attcctcgta ccctccatat gtctatgctg ggaatcagag
     5521 atttatctgt tattgaaact ccgccgacta atcgctatcc tgttcagacc tatgttttgg
     5581 aaaagaatga tagtgtcatt cgtgatgctg tcttgcgtga aatggagcgt ggaggtcaag
     5641 tttactatct ttacaacaaa gttgacacaa ttgttcagaa ggtttcagaa ttacaggagt
     5701 tgattccgga ggcttcgatt ggatatgttc atggtcgaat gagtgaagtc cagttggaaa
     5761 atactctatt agactttatt gagggacaat acgatatctt ggtgacgact actattattg
     5821 agacaggggt ggacattcca aatgctaata ctttatttat tgaaaatgcg gaccatatgg
     5881 gcttgtcaac cttatatcag ttaagaggaa gagtcggtcg tagtaatcgt attgcttatg
     5941 cttatctcat gtatcgtcca gaaaaatcaa tcagtgaagt ctctgaaaag agattagaag
     6001 cgattaaagg atttacagaa ttgggctctg gctttaagat tgcaatgcga gatctttcga
     6061 ttcgtggagc aggaaatctt ttaggaaaat cccagtctgg tttcattgat tctgttggtt
     6121 ttgaattgta ttcgcagtta ttagaggaag ctattgctaa acgaaacggt aatgctaacg
     6181 ctaacacaag aaccaaaggg aatgctgagt tgattttgca aattgatgcc tatcttcctg
     6241 atacttatat ttctgatcaa cgacataaga ttgaaattta caagaaaatt cgtcaaattg
     6301 acaaccgtgt caattatgaa gagttacaag aggagttgat agaccgtttt ggagaatacc
     6361 cagatgtagt agcctatctg ttagagattg gtttggttaa atcatacttg gacaaggtct
     6421 ttgttcaacg tgtggaaaga aaagataata aaattacaat tcaatttgaa aaagtcactc
     6481 aacgactgtt tttagctcaa gattatttta aagctttatc cgtaacgaac ttaaaagcag
     6541 gcatcgctga gaataaggga ttaatggagc ttgtatttga tgtccaaaat aagaaagatt
     6601 atgaaatttt agaaggtttg ctgatttttg gagaaagttt attagagata aaagagtcta
     6661 aggaagaaaa ttccatttga tatttttctt ctataaaata gataaaaatg gtacaataat
     6721 aaattgaggt aataaggatg agattagata aatatttaaa agtatcgcga attatcaagc
     6781 gtcgtacagt cgcaaaggaa gtagcagata aaggtagaat caaggttaat ggaatcttgg
     6841 ccaaaagttc aacggacttg aaagttaatg accaagttga aattcgcttt ggcaataagt
     6901 tgctgcttgt aaaagtacta gagatgaaag atagtacaaa aaaagaagat gcagcaggaa
     6961 tgtatgaaat tatcagtgaa acacgggtag aagaaaatgt ctaaaaatat tgtacaattg
     7021 aataattctt ttattcaaaa tgaataccaa cgtcgtcgct acctgatgaa agaacgacaa
     7081 aaacggaatc gttttatggg aggggtattg attttgatta tgctattatt tatcttgcca
     7141 acttttaatt tagcgcagag ttatcagcaa ttactccaaa gacgtcagca attagcagac
     7201 ttgcaaactc agtatcaaac tttgagtgat gaaaaggata aggagacagc atttgctacc
     7261 aagttgaaag atgaagatta tgctgctaaa tatacacgag cgaagtacta ttattctaag
     7321 tcgagggaaa aagtttatac gattcctgac ttgcttcaaa ggtgataaaa tggaaaattt
     7381 attagacgta atagagcaat ttttgagttt gtcagatgaa aagctggaag aattggctga
     7441 taaaaatcaa ttattgcgtt tacaagaaga aaaggaaagg aagaatgcgt aaattcttaa
     7501 ttattttgtt gctaccaagt tttttgacca tttcaaaagt cgttagcaca gaaaaagaag
     7561 tcgtctatac ttcgaaagaa atttattacc tttcacaatc tgactttggt atttatttta
     7621 gagaaaaatt aagttctccc atggtttatg gagaggttcc tgtttatgcg aatgaagatt
     7681 tagtagtgga atctgggaaa ttgactccca aaacaagttt tcaaataacc gagtggcgct
     7741 taaataaaca aggaattcca gtatttaagc tatcaaatca tcaatttata gctgcggaca
     7801 aacgattttt atatgatcaa tcagaggtaa ctccaacaat aaaaaaagta tggttagaat
     7861 ctgactttaa actgtacaat agtccttatg atttaaaaga agtgaaatca tccttatcag
     7921 cttattcgca agtatcaatc gacaagacca tgtttgtaga aggaagagaa tttctacata
     7981 ttgatcaggc tggatgggta gctaaagaat caacttctga agaagataat cggatgagta
     8041 aagttcaaga aatgttatct gaaaaatatc agaaagattc tttctctatt tatgttaagc
     8101 aactgactac tggaaaagaa gctggtatca atcaagatga aaagatgtat gcagccagcg
     8161 ttttgaaact ctcttatctc tattatacgc aagaaaaaat aaatgagggt ctttatcagt
     8221 tagatacgac tgtaaaatac gtatctgcag tcaatgattt tccaggttct tataaaccag
     8281 agggaagtgg tagtcttcct aaaaaagaag ataataaaga atattcttta aaggatttaa
     8341 ttacgaaagt atcaaaagaa tctgataatg tagctcataa tctattggga tattacattt
     8401 caaaccaatc tgatgccaca ttcaaatcca agatgtctgc cattatggga gatgattggg
     8461 atccaaaaga aaaattgatt tcttctaaga tggccgggaa gtttatggaa gctatttata
     8521 atcaaaatgg atttgtgcta gagtctttga ctaaaacaga ttttgatagt cagcgaattg
     8581 ccaaaggtgt ttctgttaaa gtagctcata aaattggaga tgcggatgaa tttaagcatg
     8641 atacgggtgt tgtctatgca gattctccat ttattctttc tattttcact aagaattctg
     8701 attatgatac gatttctaag atagccaagg atgtttatga ggttctaaaa tgagggaacc
     8761 agatttttta aatcattttc tcaagaaggg atatttcaaa aagcatgcta aggcggttct
     8821 agctctttct ggtggattag attccatgtt tctatttaag gtattgtcta cttatcaaaa
     8881 agagttagag attgaattga ttctagctca tgtgaatcat aagcagagaa ttgaatcaga
     8941 ttgggaagaa aaggaattaa ggaagttggc tgctgaagca gagcttccta tttatatcag
     9001 caatttttca ggagaatttt cagaagcgcg tgcacgaaat tttcgttatg atttttttca
     9061 agaggtcatg aaaaagacag gtgcgacagc tttagtcact gcccaccatg ctgatgatca
     9121 ggtggaaacg atttttatgc gcttgattcg aggaactcgc ttgcgctatc tatcaggaat
     9181 taaggagaag caagtagtcg gagagataga aatcattcgt cccttcttgc attttcagaa
     9241 aaaagacttt ccatcaattt ttcactttga agatacatca aatcaggaga atcattattt
     9301 tcgaaatcgt attcgaaatt cttacttacc aaaattggaa aaagaaaatc ctcgatttag
     9361 ggatgcaatc ttaggcattg gcaatgaaat tttagattat gatttggcaa tagctgaatt
     9421 atctaacaat attaatgtgg aagatttaca gcagttattt tcttactctg agtctacaca
     9481 aagagtttta cttcaaactt atctgaatcg ttttccagat ttgaatctta caaaaactca
     9541 gtttgctgaa gttcagcaga ttttaaaatc taaaagccag tatcgtcatc cgattaaaaa
     9601 tggctatgaa ttgataaaag agtaccaaca gtttcagatt tgtaaaatca gtccgcaggc
     9661 tgatgaaaag gaagatgaac ttgtgttaca ctatcaaaat caggtagctt atcaaggata
     9721 tttattttct tttggacttc cattagaagg tgaattaatt caacaaatac ctgtttcacg
     9781 tgaaacatcc atacacattc gtcatcgaaa aacaggagat gttttgattc aaaatgggca
     9841 tagaaaaaaa ctcagacgtt tatttattga tttgaaaatc cctatggaaa agagaaactc
     9901 tgctcttatt attgagcaat ttggtgaaat tgtctcaatt ttgggaattg cgaccaataa
     9961 tttgagtaaa aaaacgaaaa atgatataat gaacactgta ctttatatag aaaaaataga
    10021 taggtaaaaa atgttagaaa acgatattaa aaaagtcctc gtttcacacg atgaaattac
    10081 agaagcagct aaaaaactag gtgctcaatt aactaaagac tatgcaggaa aaaatccaat
    10141 cttagttggg attttaaaag gatctattcc ttttatggct gaattggtca aacatattga
    10201 tacacatatt gaaatggact tcatgatggt ttctagctac catggtggaa cagcaagtag
    10261 tggtgttatc aatattaaac aagatgtgac tcaagatatc aaaggaagac atgttctatt
    10321 tgtagaagat atcattgata caggtcaaac tttgaagaat ttgcgagata tgtttaaagc
    10381 aagagaagca gcttctgtta aaattgcaac cttgttggat aaaccagaag gacgtgttgt
    10441 agaaattgag gcagactata cctgctttac tatcccaaat gagtttgtag taggttatgg
    10501 tttagactac aaagaaaatt atcgtaatct tccttatatt ggagtattga
//
LOCUS       DAVHIT010000068        10065 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVHIT010000068 DAVHIT010000000
VERSION     DAVHIT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.069"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(83..1297)
                     /gene="thiI"
                     /locus_tag="VVN16_001584"
     CDS             complement(83..1297)
                     /gene="thiI"
                     /locus_tag="VVN16_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU5572930.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(1306..2448)
                     /locus_tag="VVN16_001585"
     CDS             complement(1306..2448)
                     /locus_tag="VVN16_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5572931.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLVGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            2586..3014
                     /locus_tag="VVN16_001586"
     CDS             2586..3014
                     /locus_tag="VVN16_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572932.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            complement(3058..5283)
                     /locus_tag="VVN16_001587"
     CDS             complement(3058..5283)
                     /locus_tag="VVN16_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5572933.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(5447..7399)
                     /locus_tag="VVN16_001588"
     CDS             complement(5447..7399)
                     /locus_tag="VVN16_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5572934.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(7396..8307)
                     /gene="pfkB"
                     /locus_tag="VVN16_001589"
     CDS             complement(7396..8307)
                     /gene="pfkB"
                     /locus_tag="VVN16_001589"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU5572935.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(8304..9044)
                     /locus_tag="VVN16_001590"
     CDS             complement(8304..9044)
                     /locus_tag="VVN16_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5572936.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPYSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGINGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            9207..9294
                     /locus_tag="VVN16_001591"
     tRNA            9207..9294
                     /locus_tag="VVN16_001591"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9241..9243,aa:Ser,seq:gga)
     gene            complement(9477..9824)
                     /gene="ssrA"
                     /locus_tag="VVN16_001592"
     tmRNA           complement(9477..9824)
                     /gene="ssrA"
                     /locus_tag="VVN16_001592"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2967 a         2292 c         1908 g         2898 t
ORIGIN      
        1 aaaaagttag ttttttacct gtttttagaa aaaaaaacta acttttttta ctgattttcg
       61 ctattctttt ggattttctg aattagagta gattatcaat caagtcatca acttcatctt
      121 tttcggcttg aggtgtgatt tcagtaatca tgattccagc cactgctcgc tcaaccaagc
      181 cttcaacatc catacgcgct tcgtactgct ccgcattctt aattttagga tttgtttttg
      241 gacgatctgg tgcaaaaatg gtacaacagt cttcaaacgg ttggattgaa atgtcaaagg
      301 tatcgatttc ctgggcgatg tcaatgattt ccaacttgtc catggtaacc acaggacgaa
      361 tgatgggagt gttggtaaca gcattgatag ccttcatact ttcaagggtt tggctggcta
      421 cttgacctag actttcccca ttgatgataa ccaaaccatt tcgtacctca cgaatacggt
      481 cagtaatccg catcataaag cgacgagtta gagtcatcaa ataagcttct ggcgctttgg
      541 ctttgatttc ctcttgaatc tctgtgaaag gcacctcgat aaactggata tttccgccaa
      601 acttggtcaa tttacgagtc aagtcctgcg ctttcttgag ggcaccagga ctagtatatg
      661 gtggactagc aaagtgaact gcctcgatat ccaccccacg cttaagagca agataacctg
      721 ctacaggtga gtcaatccct cctgacaaca tgagcatccc tttacctgaa gttccaactg
      781 gcaaaccacc agccccacga atggtttcat aagaaagata ggctgcttct tcacgaatct
      841 ccacctgaag attgatgtca ggacttttca tttgaacttg cacatttgga atggcttcga
      901 atacagcccc tccaagtgtt tggttgagtt cacgactatc aagttcaaag ttgtggtcgc
      961 tacgcttgct agaaatctta aaggtcatac cttccttgta gatgtcccgc ataatctctt
     1021 ggacagaaga cttcaaaact tctacagatt tttcaacctt ataaacagga gaaaagtttt
     1081 gaattccaaa aacttgtttg agagattctg caactgctgt gtaatcagct ccattgaggt
     1141 aagcgtgggc acggtcgcga tctgctgtta ccttaacttg agtatagata gacaaaacgt
     1201 ccgaaatatt attacgaagt ttattgatga aacgcatacg gtttttaccc ttggttgaca
     1261 actctccgta gcgaatcata atttctgaat actgcatgaa tgctcctatc ttacttttct
     1321 agtttgattg taaatcaatt ttaacttggt caaaaactgc tcgacctgac tcatatcatt
     1381 ttccaagtct aggctaagac gcacagctga cttggcctta tctttgtcca ctcccatggc
     1441 aatcaaggta ccagctggtt ttcctgcctt agatgaacaa gctgaggttg ttgagataaa
     1501 aatatcatag tcttcaaagg cgtgaacgat gacttcacct cgaacacctt tgattccaaa
     1561 agtcagaata tgaggtgcaa agtcttcctc atctgaaaag acaaaaatat ccggatagtt
     1621 cagaagagct tggtgaatca ctgccttcat ctgcccagtc ttgctcctaa agatatctag
     1681 cttttccata gataaacgaa gagccttggc tgtcgctgca atccctgcca cattttcagt
     1741 tgtcgaacga taatctcgct cctggccacc acctgtaaga agaggtgtaa tcttcttgcc
     1801 agatttgata tagacaaagc caacaccacg gacaccatga aatttatgac tcgagaaggt
     1861 cgcgcaatcc acccgttctg tcagatactt ttcagtcgga attttggcaa gcgcctgaac
     1921 cgcatcaacg tggaaggaaa tagtcggctt gtctgccaag aattctgaaa tagcctcaat
     1981 aggttggata gagccgattt cattgttcac agccatgatg gaaacgagga ttgtatcagg
     2041 ccgtatcaaa cctgctaacg cctcaacatc gaccaagcct ttcttatcaa ctggagcaaa
     2101 atccacttca aatccttgac ttttcaacca gagggctgac tctttgactg ctggatgttc
     2161 aatggctgaa acaatgatgt gcttgccaaa ctgagctttt tcaaaggcca cacccttgat
     2221 aacccagtta tccccttctg ttccaccaga ggtaaagaag atttcatcgc ttttcttacc
     2281 gactaaatct gcaatctgtt ggcgagaagc atctaagatt cgtgttgctt ggtctcccaa
     2341 acgatggaga ctagatgggt ttcctaaaat ttttgaagcg acctgcatat aggtttcaag
     2401 ggcttcagga taaggcttgg tcgtcgctga attatcaaag taaatcatgt ttctcacgct
     2461 ttctaaaatc actccctcta ttgtatcatg aaacggagct tgcgacaaga aagggcaatt
     2521 tctctttttt taagattttt ttaaagaaac agagtataat aagtcataat caaaggagag
     2581 aatgtatgtc taactatcgt agaacttcaa aaccgaaaac agaacatatc aaaaaaggct
     2641 ttactgtttt tcaaaaaacc attactacta tcggtagtat ccttggtttg attaccgcgg
     2701 gtatcacgat catgaacgcc ttggataata ataaaaatac taaaaaagaa cctacgacaa
     2761 gccagacgac aacctttgtc aaagaaattc aaaaagaatc ccctcaggaa aatactactc
     2821 caaataagga aaataacact tctcaagaaa aaacacaaca agaagaaacg ccaaaatcta
     2881 gcgtcaagga agagaaaaaa gaagatcaga aaacagcaac tcaggactct actacacctg
     2941 ctacaagtaa acctgccact gaaaatgaaa aacagcccaa tactccaact tcagaaaata
     3001 aaagtaatca ataaccagaa aaatagcttc cttccaacct tggaaagaag ctatttttta
     3061 ttgctgcaat acttttcttg gcttggtacc ttcagctgga ccaatgacac ctgccatctc
     3121 aagctcttcc atgagacggg tcgcacggtt aaatccaact gacaaacgac gctgaatcat
     3181 ggatgcactg gctttctgtg tttcgataac caaagactta gcttcttcaa aaagcggatc
     3241 accaccagca tctccatccg aaaattctcc ttcattttca gaaacctcac ctggatcaaa
     3301 actctcatcg tagtctgcat ctgcctgagt cttgatgaag ttcacaatac gctcaacatc
     3361 gtcatccgag ataaaggagc cttggagacg aactggatga ttttcatcaa tcggtttaaa
     3421 gagcatgtct cctcgaccaa gaagtttttc tgctccattt tcatccaaaa tcgtacggga
     3481 gtctgttcct gatgaaaccg caaatgctac acgagatgga acattggcct taatcaaacc
     3541 agagatgaca tcaacagatg ggcgctgagt tgcaagaatc atgtggatac ctgcagcacg
     3601 cgccttctgc ccaagacgga tgatagcatc ttccacttcc ttgctggcca ccatcatgag
     3661 gtcagccaac tcatccacaa tcacgacaat gaatggtagc ggaatttgct tgtactcaga
     3721 ctgggaattg aactcttcta ccttggcatt aaaacctgca atattccgaa ctcccacctt
     3781 ggcaaagagt tcataacggt tttccatttc atccacaacc ttttgcagag ccttgctggc
     3841 tttacgtgga ttggtcacaa ctggaatcaa gaggtgggga atatcattgt aaacagataa
     3901 ctcaaccatc ttgggatcga ccatcataaa tttaacttga tctggtctcg ccttcatgag
     3961 aatgctagca ataatgccgt taactgctac tgacttccct gaacccgttg aacctgcaac
     4021 tagcaagtgg ggcattttag aaaggtcaaa agctcttgcg gttccattaa cagccttccc
     4081 taaaggaatt tccaagaaat tttctgcttt cgtttgcgat tgttcccata gttctcggaa
     4141 agatacagtg gcaatatcgg agttgggcac ttcaattccg attagggatt tcccaggaat
     4201 tggtgcttca atccggacat ctttggcagc caaggctaga gcgaggtcat ctgatagatt
     4261 ggaaatgcgg ttgaccctta caccaacagc cggcttgact tcatacttgg tcactgatgg
     4321 cccaatttcg gcccgttcaa ctgttacctt aataccaaag ctagcaaagg ttgcttctaa
     4381 gattttgata ttttctctga caattttctt ctctttagac tgatcttttg gtttatctgg
     4441 tgcaaagagt tgtaagcttg gaagtttgta ttcaagggct tctttggctg aaaaatcgac
     4501 ctgaacatct tcgtcatctg agtcatcttc ctgttcaggg aatttaagtt cagcttgagg
     4561 caggatgatt tctggttcca cccacttttc ttctggaata ggtggaagat tttgaacagc
     4621 ttcctctgtc agaatttcac ccgtttccat atcaacagga ggcaaatcga gtaaggcttt
     4681 ttcagtctct tcttgttcta atctagcctc tttctcagcc ttttggcgag ctttttcttc
     4741 ttgtttgaca aagcgttcct cttttcgacg ctcgtgccct tcccaccatt tggcaaagcc
     4801 tctactgaaa aattcagcaa tatcgtaaac agaccaaggg ctgactagga gagaacccac
     4861 taaaatcaag atagaaccaa taaagtaagt tccgatattt gaaaagagaa aggctgttgg
     4921 aatataaaga gcgaccccga tcaagccccc tccagcaaag ctagtcgttc gaaaaccagt
     4981 cagatctgtc acaacctgag ccatggtccc ttttagaacg gacttgtcca aaccatattt
     5041 ccaaaccaag taggcttcaa aaatcaagag taagccagca aatatggtga aaaagccaga
     5101 taagagtcct tcctgttttc gtatccactt gaaaaagaag agatagatta ataggccgaa
     5161 tatcgccaga taagctaggc tacccactag caagcgaatt aaattataaa gggttatacc
     5221 tgcagcccct aatttgaagg ctgcgaaaat caataaaatc gcaattccta acgaaatcaa
     5281 cattcgttga atcgcttctt ttctttccag ttctgcttta gacggtctcc gtcttgttgt
     5341 acttgtattc ttgtttgcca ttcttctatt ataccatatt tcacaggcat ttcgggaaga
     5401 gaaaaagact gcaccaaacg gttcaatctt ttcatttttc tattttttat gcttgtggtt
     5461 tgcgtaggta accataaacc acaccactta cgattgctcc taccaagaca gaaacgaggt
     5521 aaaggagagc atttgaagta agggcgataa cgaagattcc tccgtgtggc gccatgagtt
     5581 tgataccagt aagaccaacg agtccacctg ctactgctga accaaggatg aagcttggaa
     5641 tcgcacgagc tgggtcagcg gcaccaaatg gaatcgctcc ctcagtgata aatgacaagc
     5701 ccatgatgat gtttgtcaaa ccagagttac gttcttcctt agtaaattta tctttgaaaa
     5761 gaagagttgc gacaaagatt gcaagtggtg gcaccattcc tccagccata actgctgcca
     5821 tggctacaga accacctgaa gaaacagttg ctgcaagcgt acctgtacca aagacataag
     5881 ctgctttatt aactggtcca cccatatcaa cagccatcat tccaccaagg acgataccaa
     5941 gaaggacagc tgaacctcct ccaagaccgc ctaggaagtc attcatagca gtgttgattg
     6001 cagccattgg gatatttaca gctagcataa caaatcctgt caagattgtt ccaagaagtg
     6061 gcaatagaag gattgattta gcaccttcga gtgaacgagg aactttaacg tatttcttga
     6121 tggcaagaac caaggcacct gcgataaatc caccaacaag ggcacctagg aaaccagatg
     6181 agacacctgc aagagttgaa gttgcttcac cacctgcggc ataaggaatt ttaccaaagg
     6241 caaaaccttc tttggcaata gcaccagcca cgaaacctgc taccaaacct ggtttttcag
     6301 caatagagta ggcaacataa cccgcaaaga ctggaagcat caaaccaaag gcagctccac
     6361 caattttcat gaacatagaa gctaactcat ggtaagaacc aagattgcca aggttttcat
     6421 ttggaacacc caaagcaccg tcaatcaaga aggcaagggc aatcatgata ccaccaccga
     6481 taacgaatgg taacatttga gatacaccac tcatcaagtg tttgtagaag gcaccaccaa
     6541 ggctttgttt ttcgttagag gctgttgcag cttttgcacc attagcggca cggtagactt
     6601 cagtatctcc tgaaagagcc aagttaatta gctcttctgt cttacggata ccgtcagcaa
     6661 ctggacgatt gatcaatggt tttccatcaa atcgatccat ttcaacggcc ttgtctgctg
     6721 caatgataat agctttagcc ttacggatat cttctgcagt tagttgattt ccaacaccgc
     6781 tagcaccgtt ggtttcgacc ttgataccaa cccccatttc agcagctact ttttgaaggg
     6841 cttcttgggc catgtaagtg tgggcaatac ctgttgtaca agctgtaaca gctacgataa
     6901 agtcaccaga gtcattagca ggtgcttgaa caagttcctc agttttttct gaagcttggt
     6961 caaaaagttc gataacttgg tctgcagatg ttgcttgacg aagtttgtct gcaaaaccgt
     7021 ctttcatcaa gtattgagac aattctgcca aggctgccaa gtgagtatca ttggcacctt
     7081 ctggagctgc aatcatgaag aagaggtcag ttgcttgtcc atccaagctc tcgtagtcaa
     7141 cacccttatt tgacttagca aatagaactg tcgcttcttt gacagcagcg tttttgctgt
     7201 gaggcattgc gattccatca cccaaaccag tagaagtcaa agcttcacgc gccaaaattc
     7261 cttctttaaa tgtttcaaaa tctgttacat aaccgtggtc tgtcaaattt ttaatcatct
     7321 cgtcgatgac agctgttttt tcagttgcct gcaaatctag caacatgaca tcttttctca
     7381 ataggtcttg aattttcatc gtttttctac ctcaactttt ccatatgttt ctttaataaa
     7441 ttccgccgtt gccaagtcat ctgagaaggt agttgccgtt ccgcaagcca ctccccattt
     7501 gaaggcttct actgcgtctt ttgatttgac aaattcacct gtgaatccag caaccataga
     7561 atcaccagct ccaactgaat ttttgactgt tcctttgatt ggtttagcga agtaagctcc
     7621 ctcagatgtg acaagaaggg caccatcacc agccatagag ataataacat tttgagcacc
     7681 cttagccagt aactcacgag cgtatttctc aatttcatct aaactttcga gtttaacccc
     7741 aaaaatcgct ccaagttcat gattgtttgg ttttacaaga agaggctggt agtccaaact
     7801 atcaattaag gtctgtcctt caaagtcaca gaccacttgc gcaccagtct ggcgcgtcaa
     7861 ggaaatcaaa tccttataga taacattgcc tagattttta gcacttgaac ctgcaaagac
     7921 aactgtatct tctgctgtca gactagataa aatagctttc aattcttcta gctgaaccga
     7981 ttcaacagtt ggacccgttc cgttgatttc tgtttcttgg tctgctttga ttttaacatt
     8041 gatacgagta tcttctgcca cctggacaaa acgtgtctcg atttcttcct ctgctaaagt
     8101 atctgtgata aatttaccag taaagccacc gataaatccc gtcgctgtat ttggtatatt
     8161 caaacgtttc aagacacggc tgacattgat tcctttccca ccagcaaact tatcatcact
     8221 gtccatacga ttgacactac caactttgac ttggtccaaa cgaacgatat agtcaatgga
     8281 tggattgagt gtgactgtat aaatcatact tctattacct ccgttttctc cttaataacc
     8341 tgcaagagct catgcccttg actagtgata acgatagcgc gtttgagtgg ggctaccttg
     8401 gcaaagcaag tttgtccaat ttttgacgaa tccaccaaga cgtaggtctg cttggcattc
     8461 tccaaaatag ctcttttcac agctccctcc tccatatcag gagtcgtata atagccatcg
     8521 tcaacaccat ttattccgat aaaggcacgg tcaaagtgca attggttaat ctggttaaga
     8581 gcaacgcccc cgatactagc atctgtcgcc gtcttgacgt ttcctccaac catgacagtt
     8641 ggaatctgct tttcaaccaa ctgagcggca tggtgaatgg agttggtcac aactgtaaca
     8701 ttcttattga ccaattcatg aatcaaaaaa gcagttgttg ttccagcatc gataaagatg
     8761 acatcttttt ctttaatgag agaggctgct ttctgagcca gcaatttctt ttcttgaagg
     8821 tttttgacag atttttcttg aatggtttct tcttcctgta aggagtaggg gagttctgct
     8881 ccaccatgca cacgacgaag cttgttttcc gcttccaact catccaagtc tcttcgaacc
     8941 gttgattctg acgtttctag caaactaact aatttttcta gagaaactac atgatgttga
     9001 tttaactcct ctaaaattag ttgtttacgt tctgttttta acactgaatc acctcctgtt
     9061 atcgtttaca ctattcattc tatcatactt tctttcgaag tcaagcattt tttatcattt
     9121 tctttcaaaa tctatcattt tttcttattt ctagaatttt tattgcaaga taagagcctt
     9181 tatggtagac tatttgagta agtattggaa gattactcaa gaggcttaag aggccgtgtt
     9241 ggaaacgcgg taggcgtgta atagcgtgcg tgggttcgaa tcccatgtct tccgtcgaaa
     9301 aaagattatt tctaggatgc gacaaatgtt gtatcctttt ttctatcctt tttttaaaat
     9361 agggtctgtt tgtgactccc tttttagcaa aagaatgcaa aggaatacca aagataaaaa
     9421 taaaaaacgt tgatttaaca acgttttacc aaggaatgca aaagaatgca aaggaataat
     9481 ggagccggtg ggagtcgaac ccacgtccaa acacctgcca acatatttgt ctacaaccat
     9541 aggttatgta ttattttaac agcccctcga cacataactc aagtctagaa actgcgagtc
     9601 tatcaatctc ttatcaagcc gctagacaag acttaatcgt atctcgctaa taataagacc
     9661 tgtcattgaa cacgagcaat ccaaatcggg tcacgcctgc tggtttttaa gcagctagag
     9721 cgtaagaagt gttatttttt gcagttatat ttaactgagc gtttacgtcg ccacacgagt
     9781 cgcaaaatat gcctcataat gcctgtcgaa tccgtaacga ccccaaaaga caataaaacc
     9841 attatacctt atctaactca aaaatgcaaa aagaaatcac atacctaaaa aagatactat
     9901 tcgagaaatt tagcaaactg gaagactaac tacaactttc tcagtgcata actttacgat
     9961 tgtaaataga gcttacgaaa tccaattaac acatacctaa gatgaagcta tctgttttga
    10021 agaaattcac gaagagaatt attctaaaat actttcagat gagaa
//
LOCUS       DAVHIT010000069        10038 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVHIT010000069 DAVHIT010000000
VERSION     DAVHIT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.070"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..2428)
                     /gene="phtA"
                     /locus_tag="VVN16_001593"
     CDS             complement(<1..2428)
                     /gene="phtA"
                     /locus_tag="VVN16_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU5572937.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTETHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEVEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSS"
     gene            complement(2681..4414)
                     /gene="ptsP"
                     /locus_tag="VVN16_001594"
     CDS             complement(2681..4414)
                     /gene="ptsP"
                     /locus_tag="VVN16_001594"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU5572938.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(4420..4683)
                     /locus_tag="VVN16_001595"
     CDS             complement(4420..4683)
                     /locus_tag="VVN16_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU5572939.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            5034..5252
                     /gene="nrdH"
                     /locus_tag="VVN16_001596"
     CDS             5034..5252
                     /gene="nrdH"
                     /locus_tag="VVN16_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU5572940.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            5333..7492
                     /gene="nrdE"
                     /locus_tag="VVN16_001597"
     CDS             5333..7492
                     /gene="nrdE"
                     /locus_tag="VVN16_001597"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU5572941.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            7680..8642
                     /gene="nrdF"
                     /locus_tag="VVN16_001598"
     CDS             7680..8642
                     /gene="nrdF"
                     /locus_tag="VVN16_001598"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU5572942.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADD
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEVMQDDDYNYGLD"
     gene            8824..9585
                     /locus_tag="VVN16_001599"
     CDS             8824..9585
                     /locus_tag="VVN16_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5572943.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9864..>10038)
                     /locus_tag="VVN16_001600"
     CDS             complement(9864..>10038)
                     /locus_tag="VVN16_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU5572944.1"
                     /translation="SEELKSLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEWDT
                     ISSDSDFMPFQQS"
BASE COUNT         2949 a         2070 c         1855 g         3164 t
ORIGIN      
        1 cagatgaagg attacttcct tttaacaacg caagtaattt ttctgcttct gccatgatac
       61 tattgttatc cataatttga agagtcaaat tatttcgtaa accagctaga gtttctgttg
      121 cattggcttt cagactagaa tccgttactt tcgcaagcaa aacttctact tctttgagtt
      181 gggcttctac tttttcagtc tctacttgag ggacttctgg ctcagcaggt gtttcctcta
      241 ctggctcttc atccgctttg aagttcttat ttggatcttc actgtggtct ttcttgccta
      301 acacatgctc actggcattg ccccatccat cattagaatg tggacgttcg tcagggtgtt
      361 ctacgtagta cttaatcgtc gcaaacaaat cttccaaggt atagccattt ggagctttgt
      421 atgtgtgatc atcaaaccaa gcaaatttaa tgttatggta atgatcctta tgaggaataa
      481 tcaaattacc gtttttaacc tcaactgtat gctcaaccat atatggaagt cgaacgagtg
      541 gaattcgttt ttcccctttc acacgattgt aaatagctgc tgcactatct ccagttggat
      601 ttgctttaac atctgcgtct ggagacggag gtaggatacc tttttcttta gtataggctt
      661 gagctgcaac tttttcctta tcagaaaggc tgtcttttcc aatccagtga ctatggccca
      721 tatgaggcgt tacatatgca tctccttcat cactgattat atcatgttca tcaaaaatat
      781 aaccatctga cgttgtatac ttatcagcta attgagcaat acgaacttcg tcttcagtat
      841 actcaatttg agaatttggc ttgccaagtc gctctggatg ggtaattggt gctaggaatg
      901 ccaataaatc atctaccaat ttttctttat tagtcgattc atcattcaag cgttctaata
      961 atttgtctaa ggcttggaaa tcagaattac gacccttatt ttcaaacaag gctttatgag
     1021 tctcagttaa cagattatat gctttatcat aaaattcttg gtcacgagga gcaacatttt
     1081 cttttttagc agttaaagtg tgtgaaacac tctcttgttt tgataacttg ctttcaagat
     1141 ttttaacagt ttcagatggt aaatctttcg caaagacata acgagagatg cccttttctt
     1201 cgaatacata tccttcccca acttttcgta ccagctgact aaccaaagaa gaatttgagt
     1261 ctattttaag atttggtgca ggttgcgggc ctggactagg ttccggagtc ggttgtggac
     1321 ttggttgttc tggcctggaa tctggtaccc aatggtttga acgataacga aggggaataa
     1381 tacgagcgat tcgttcttcc aattcagaca tttgagagta agggatgaag tggtaatgat
     1441 ctccgtgtgg cactgcaaca cctctagctg ttcgacttgt gatttgcgct gggtcgaaaa
     1501 caaggccatc agattctaca tggcgttgac tcaaaggcag tttgtagagc tgtttcaaga
     1561 gactatcaat gtcattactt tgacttgctt gactgttagt gttgctgttg ttgcttgtgt
     1621 tagtatttgt agttcctgga ttgcttacag aaggtaccca gtttgttctt gaagtgttat
     1681 cgctattttg tcggcgatag gttcttgaat ttgacagatt tccccgacca gataggaagg
     1741 cttctgcagc agctaactcg ctagctgata actcattctt aggaatgtaa tggtaatgat
     1801 ctccatgagg aacgatataa gcatcaccag tatcctctat gatatcagaa gcattaaaga
     1861 tataaccatc atctgtagta tagcgtcctt gcgaacgtgc caaggcaaca gcaccatcgt
     1921 ttcttggagt cccaccttca cgatgttgac tatgctcttg tttttgtcga ttgatttcct
     1981 cttttgtacg gacgttatcc gcgtgggcag catccttaag gtaaacatag tattttccat
     2041 ctaccttgat aacatatcca cccttgacct cattaacaat atcctcatct tttagcttat
     2101 agtttggatc tttcatgagt aattcttcac tgaaaatcgc atcataagga accttcccat
     2161 tgtaataatg atagtggtcg ccatgtgaag tgacatagcc ttggtctgtt atcttgatga
     2221 cgatttgctc agcattgatt ccttcacgct tgctaacctc atcaggagtc aaattctccg
     2281 ttttttgcgt cgcttgtttt ccatctatat aggaaacacg attattttcc ttaaccgttc
     2341 tagcttgata cagtcccaac tcgtaagaac aaacacttaa aatcaaagct gccgcagaac
     2401 caacaaggta tttcttatta attttcatcg aaactctctt tctatttgat atttctagtt
     2461 gcaattcatt tctaaattgc agaaactcct ctctattact taactggtta atagtttact
     2521 cctaaatctc caacttgtca agaattttat gaaccattta agtatttttt gttctgacca
     2581 ctactttttc aaggttggac tttctataag aaagattttc ttgaaaattt ttttaaaata
     2641 tcaaaaaaat ccctgtaact gagttgcagg gacttttcga ttaatcaaaa ttaacgtatt
     2701 ctttttgaag ttcaagaact tcttccattg ttgagcattc tgtaagggca cggtttgcgt
     2761 actcttccat cttagctgtg tcgagtttct tcatcaagct gcgtgtacga agtacagatg
     2821 ttgctgacat agagaactca tccaagccca ttccgacaag aagtggaaca gcttgttggt
     2881 caccagccat ctcaccacac ataccagccc atttaccttc agcgtgagct gctttgatca
     2941 cattgttaat caagcgtagg attgatgggt tgtatggttg gtaaaggtat gaaacttgtt
     3001 cgttcatacg gtctgctgcc attgtatatt ggatcaagtc gtttgtacca attgagaaga
     3061 agtcaacttc tttagcaaat tggtctgcaa gcatagccgc tgcaggaatc tcgatcatga
     3121 taccaacttg gatgttatcc gcaactgcaa caccttcagc aagaaggttt gctttttctt
     3181 catcaaagac tgctttcgct gcacggaatt ctttcaagag cgcaaccatt gggaacatga
     3241 tacgcaattg accgtgaaca gacgcacgaa gaagagcacg gatttgtgtg cggaacatag
     3301 catctccagt ctcagagata gagatacgaa gagcacggaa tccaaggaat gggttcattt
     3361 cgtgaggcat atcgaagtaa ggaagttcct tatctccacc gatatccatt gtacgaacga
     3421 caacaggttt accgttcatt ccttcaagaa cagccttgta tgcttcatac tgctcatctt
     3481 cagttgggaa gtcttgagaa tccatgtaca agaactctgt acggtaaagt ccaacagctt
     3541 ctgcaccgtt gttgttaaca ccttcaacgt cttttggagt accaatattg gcagccaact
     3601 cgaagtgttt accgtcagca gtcactgttt gagcatcttt caaaagtgcc cattcagctt
     3661 tttgtttcgc ataggcttca ccagctgctt taaattctgc cgcttgttca tctgttgggt
     3721 tgataatcac ttctccagtg atcccgttaa cagcaaggat gtcaccgtct ttaacgattt
     3781 cagtgatgtt gtttgtacct aatacagcag caatttcaag tgtacgtgcc atgatagctg
     3841 agtggcttgt acgtccaccg atgttggtta caaaagcttt tacaaagttt ttgtccaatt
     3901 gagctgtatc tgaaggagtc aagtcatgcg caatcacaat cacttcttca ttgatagaag
     3961 ctgggtttgg caatttttta ccaagaaggt ttgccaatac acgttttgtc acgtcgcgga
     4021 tatccgctgc gcgttcttgc atgtatgggt tgtcttccat gccttcaaag atagtgataa
     4081 acatatctgt aacttctttc agacctgctt ctgcattcac tttcttcgca cggatagttt
     4141 ccttgatttg gctgatcatt tctgggtcag caagaaccat taagtgagca tcaaaaactt
     4201 gagctgcttc ttcaccgagc gtacctactg ctttctcgcg aataacagaa agctcgtctt
     4261 gtgatgcctg tagagcggca tcaaggcgag cttcttctgc gtttgtatct tcgactgtaa
     4321 tagtctcaaa tgacaaatcc ggctgaacga gtagatatgc ttttgcaact gcaacaccgt
     4381 cagatgctgc gattccttta agcatttctg tcatttccct tatgccaatc cttctttttc
     4441 cattgtttct gagattgcag cgatagcgtc atctgcatct gcaccttcag ctgagatagt
     4501 tacgtcagca ccttggccaa caccaagact cataacaccc ataattgatt taaggttaac
     4561 tgatttacct ttgtactcaa gagtgatatc tgaagcaaat ttgctagcag tttgtaccaa
     4621 caatgttgct ggacgtgcgt gaatacctgt ttctgccact acgtggaaat ctttagaagc
     4681 catagtttga ctctcctttt gttctttctt tttttgagtt atatgtgata acccttacaa
     4741 tttagtatta taccatcttc taggattttt ttcaagcatt ttctggattt tccttcaatt
     4801 tcctttcaga taacttgctg aaaatcttct atcctagata acaaaacaca ggatatagtt
     4861 tctttaaaaa caatttatag gataatcatt gctatttcac aatacaaaca ctatatattg
     4921 tgtttatata taaaaacaac aaaagaacac cactatattt agttcaaatc attgacaaaa
     4981 ttttattttc tgatatacta agatagtatt aaattttgaa gaggagttac acaatggtaa
     5041 ccgtttattc taaaaacaat tgtgtccaat gtaaaatgac caagcgtttc ttggacagta
     5101 ataatgtctc ttatcgtgaa atcaatcttg atgagcaacc tgagtacgtc gatcaagtta
     5161 aagagctcgg ttttagcgca gctcctgtta tccaaacacc aactgaagtc ttttcaggtt
     5221 tccaaccagg aaaactgaaa caattagcat aatcttagta catcatccag aagaaactgc
     5281 ttttagggct aacttagaag cctttctttt gtaattagat aagggaaatt ttatgggatt
     5341 aaaacatctt gaagacgtga cttacttccg tcttaataac gaaattaacc gtcctgttaa
     5401 tggacaaatc atgcttcata aagacaagga agccttggat gctttcttta aagaaaatgt
     5461 agttccaaac actatggttt ttgattcaat caaagacaaa atcaattacc tcattgaaca
     5521 caactacatc gaaacagcct ttatcaagaa ataccgtcca gagttcttgg aagaattggc
     5581 tcaatttatc aaagaccaaa acttccaatt caagtcattc atggcagctt ataaatttta
     5641 caatcaatat gccttaaaga ctaatgacgg tgaatactat cttgaaaata tggaagaccg
     5701 tgtcttcttc aacgcccttt atttcgctga tgggaatgaa gctgttgcaa tcgatattgc
     5761 caatgaaatc atccaccaac gctaccaacc tgctactcct tccttcttga atgctggacg
     5821 tgctcgtcgt ggggagttgg tatcttgttt cctaatccaa gtgacggatg atatgaactc
     5881 tatcggacgt tctatcaact cagctcttca actttcacgt attggtggtg gtgtgggaat
     5941 taccctcagc aaccttcgtg aagctggtgc acctatcaaa ggctatgaag gagctgcttc
     6001 aggtgtcgtt cctgttatga agctttttga agacagcttc tcttactcca accaattggg
     6061 gcaacgtcaa ggtgccggtg ttgtctacct caacgtcttt cacccagata tcatcgcttt
     6121 cctttcaact aagaaagaaa acgctgatga aaaagtacgt gtcaagactc tatcacttgg
     6181 tgttgtagta cctgataaat tctacgaatt ggctcgtaaa aatgaagaaa tgtacctctt
     6241 cagcccttac tctgtagaaa aagaatacgg tgtgccattc aattacatcg acattactga
     6301 aaaatacgat gaattggtcg caaatccaaa tattcgcaag acaaaaatca aggcgcggga
     6361 tttggaaact gaaatctcta aattgcaaca agagtctggc tatccttatg tagtcaacat
     6421 cgatacagct aaccgtgcaa atcctgttga tggaaagatt atcatgagta acttgtgttc
     6481 tgagattctt caagttcaag aaccaagctt gatcaacgat gctcaagaat tccttcaaat
     6541 gggaacagat gtttcatgta atctgggttc aaccaacgtg gttaacatga tgacttcacc
     6601 tgattttggt cgttctatcc gtgctatggt tcgtgccctt actttcgtta cagatagttc
     6661 acatatcgta gctgtaccaa cgattgacca tggaaatagc caagcccaca cctttggtct
     6721 cggtgccatg ggactgcaca gctaccttgc ccaacaactc attgaatatg gttcacctga
     6781 gtctgttgaa tttacaagca tctactttat gcttatgaac tactggacct tggtagagtc
     6841 aaacaatatt gcgcgtgaac gtggtattac cttccacaac tttgaaaaat cagactatgc
     6901 taacggaagt tactttgaca agtatgtaac tggtgaattt gttccaacat cagaccgtgt
     6961 taaagaactc ttcaaaaatg tttttatccc tggtgttgct gattgggctg aacttcgcga
     7021 caaggttcaa gaagatggtc tttaccacca aaatcgcctt gctgtagcgc caaatggttc
     7081 tatcagctat atcaacgacg tttctgcttc tatccacccg attacgcaac gtatcgaaga
     7141 acgccaagag aagaaaattg gtaaaatcta ctaccctgct gctggcttgt ctacagaaac
     7201 cattccttac tacacttctg cctacgatat ggatatgcgt aaagtcatcg atgtttacgc
     7261 tgctgcgact gaacacgtgg atcaaggact ttcactcacc ctcttcatgc gcagtgacat
     7321 tccaaaaggc ctttacgaat ggaagaggga aaataaacaa acgacacgtg acttgtccat
     7381 ccttcgtaac tatgccttta acaagggaat caagtctatc tactacgtcc gtacctttac
     7441 agatgacggt ggagaagtcg gcgccaacca atgtgaaagc tgtgtgattt agtttctagc
     7501 atagaaacac tatttttccc taagcagcac tgataaaatc aaatctacta tgtcataaca
     7561 taatttatgt aacattttaa aagtcgggct tccgactttt tgtgcgatac tcctatagaa
     7621 ttatggtaaa gtagaaataa ttgtgagttc gcaatactaa acacagaaag agattttaaa
     7681 tggaaactta ctacaaagcc attaactgga atgccatcga agatgtcatc gacaaatcaa
     7741 cttgggaaaa actgacggag caattttggc tcgatacacg tattccctta tcaaacgact
     7801 tggatgactg gagaaagcta tctaacaaag agaaagactt ggtcggaaaa gtttttggtg
     7861 gtttaaccct tcttgacact atgcaatctg aaactggggt tcaagccctt cgcgcagaca
     7921 tccgtacacc acatgaggaa gctgttttca ataacatcca atttatggaa tctgtccacg
     7981 ctaaatctta ctcatcaatc ttttctacct tgaatactaa ggctgagatt gaagaaattt
     8041 tcgaatggac caataccaat ccttacctac aaaagaaggc tgagattgtc aacgaaatct
     8101 acctaaacgg cagcccactt gaaaagaaag ttgccagcgt cttcctcgaa accttcctct
     8161 tctactctgg tttcttcact cccctctact atctcggtaa caacaaactt gccaacgttg
     8221 cagaaatcat taaattgatt attcgtgacg agtctgttca cggaacctac attggttaca
     8281 aattccaact tggtttcaat gaattacctg aagaagagca agaaaaactc aaagaatgga
     8341 tgtacgacct actctatact ctttatgaaa atgaagaagg ctacacagag agtctttatg
     8401 acggtgttgg ttggactgaa gaggttaaaa ccttccttcg ctacaatgcc aataaagctc
     8461 tcatgaacat gggacaagat ccactcttcc cagattcagc tgatgatgtc aacccaatcg
     8521 taatgaacgg tatttcaaca ggaacttcca accacgactt cttctctcaa gtcggaaatg
     8581 gttaccttct tggcgaagtt gaagtcatgc aagacgatga ctataactac ggtttagact
     8641 aattctccac cactctcaat catcaaaaag taaactagaa acatcaaagt tataacccaa
     8701 caaaaatatc gaaagttttg aaaacaaact tcgatatttt tgtttttgtt tcattttgtt
     8761 gtttttgtat ttgaatgatg agtttgttta tggtaagata gtagtaggaa acgaggtgat
     8821 aatatgctca aacaagaaaa attaaccaaa attttagaga tagtaaatag caaaggaacc
     8881 ataacggtta aacagattat ggacgaaata gccgtttcag atatgactgc aaggcgctat
     8941 ttacaggaat tagctgataa agatttgctg attcgtgtgc atggtggagc tgaaaaactt
     9001 cgaaccaact cccttttgac taatgagcga tcaaatattg aaaaacaagc cctccaaacg
     9061 gcagaaaaac aagaaatagc ccattttgca ggcagtctag tagaagaaag agaaactatt
     9121 ttcattggac caggaacaac attagagttt tttgcgcgtg agttgcctat tgacaatatc
     9181 cgcgtcgtaa ccaacagtct acctgttttt ctgattttaa gcgaacgaaa attaacagat
     9241 ttgattttaa taggtggaaa ttatcgcgat attacaggtg cttttgttgg cacattgacc
     9301 ctacaaaatc tctctaatct ccaattttct aaagctttcg ttagctgtaa tggtattcaa
     9361 aacggagctc tagctacttt tagcgaggaa gagggagagg ctcaacgcat cgctttaaat
     9421 aattctaata aaaaatattt actcgcagat catagtaagt tcaataagtt tgatttttat
     9481 actttttata atatatcaaa tcttgatact attgtttcag attctaaact aagtgattca
     9541 atccttttta agctatctaa acacattaaa gtcatcaagc cttaataaac tgaaagagcc
     9601 tgggtaacaa ctcaatttca gaagaaaaaa agtacatctt cccacaataa aacgcatagt
     9661 atcaggtgtt gaaacacctg atactatgcg tttttctata tcaaagactt tttgcccaat
     9721 tcctcaaagc tatacattga gaaatctgcg actcgcttcc tagtttaaag ttgctctttg
     9781 attttcattg agtataagca acgtgattag ataagagttt tacagagtac taaatataag
     9841 atttaacttt tttgagaaat tggttacgat tgttgaaacg gcataaaatc tgaatcagag
     9901 gagatagtat cccattcttc aggattgata aaatttaaaa atagattttt aaattcttcc
     9961 agctcatcat ctgaatgaca aatccattct ttaccagtcg agacatacca ttttccaaga
    10021 cttttcaatt cttcactc
//
LOCUS       DAVHIT010000070         9783 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVHIT010000070 DAVHIT010000000
VERSION     DAVHIT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.071"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            607..1599
                     /locus_tag="VVN16_001601"
     CDS             607..1599
                     /locus_tag="VVN16_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5572945.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1601..2419
                     /locus_tag="VVN16_001602"
     CDS             1601..2419
                     /locus_tag="VVN16_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5572946.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2421..3206
                     /locus_tag="VVN16_001603"
     CDS             2421..3206
                     /locus_tag="VVN16_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5572947.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3245..3523
                     /locus_tag="VVN16_001604"
                     /pseudo
     CDS             3245..3523
                     /locus_tag="VVN16_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
     gene            3460..3705
                     /locus_tag="VVN16_001605"
     CDS             3460..3705
                     /locus_tag="VVN16_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572948.1"
                     /translation="MSRRKKAYQGRKIGSQLLATLESEAHKKVGYLQVKTVAEGSNKD
                     YDRTNDFYRGLGFKKLEIFLQLWNPQNPCQILIKKLE"
     gene            4438..>9783
                     /locus_tag="VVN16_001606"
     CDS             4438..>9783
                     /locus_tag="VVN16_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5572949.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVS
                     REHFIRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEG
                     KSLFNIHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAY
                     DDGTDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEI
                     KATKYDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKET
                     KSEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFN"
BASE COUNT         3346 a         1534 c         1980 g         2923 t
ORIGIN      
        1 atgatgaaag ccttaagtgt tattttacaa aggttatttc aagtcgttcc aaggtaacaa
       61 gtctagatca gattgaagct gataaaacga tacaaagaaa atattcaagt gagctaaaaa
      121 aatttattgg attttataat gagattattt gtgaggaaaa tagtttccta catgtacgaa
      181 agaggtggtc gagttgattt aggtagtcgg tgcgtgagtt gataattctc agggtatgga
      241 cttctttttc atgaatgagg taaaagagca ggtattgttt agagacaatc attctgagca
      301 tattttctgg atagagggag tatccgattt tatgatcaaa gttaataccg ccctctggtg
      361 agaagatgag taggttggta atttaaacta ttaaacagaa tttttgatta aaagtattat
      421 ttcatgagag aaatcctaat ttcacaatcc ataggcaaac gcttgcattc tgttttttat
      481 tggactataa taggttggta taaagccttc tgtagtaata aaatgtagaa ggtgtagaaa
      541 gtaaggattt agaatatttg tagttaaaaa cacaatgttg ctattcctta tgatagggag
      601 atagatatgg caatgataga agtggaacat cttcagaaac attttgtgaa gattgttaag
      661 gaaccgggct tgaagggggc tttgcgctcc tttattcatc ctgaaaagca gacctttgaa
      721 gcggtcaagg atttgacctt tgaggttcca aaagggcaga ttttaggatt tatcggggca
      781 aatggtgctg ggaagtcgac aaccattaaa atgctgacag gaattttgaa accaacatct
      841 ggtttttgtc ggattaacgg caagattccc caggacaatc ggcaagatta tgtcaaagat
      901 attggcgtag tctttggaca acgcacccag ctatggtggg atttggctct gcaagagacc
      961 tacactgtct taaaagagat ttatgatgtg ccagactcgc tctttcataa gcgtatggac
     1021 tttttgaatg aagtcttgga tttgaaggac tttatcaagg atcccgtgcg gactctttca
     1081 ctgggacaac ggatgcgggc ggatattgcg gcctccttgc tccacaatcc caaggttctt
     1141 tttttagatg agccgaccat tggtttggac gtttcggtta aggataatat tcgtcgggca
     1201 attactcaga tcaatcaaga ggaagaaact accattcttt tgaccactca cgatttgagt
     1261 gatattgagc aactttgtga tcggattttc atgattgaca aggggcaaga gatttttgat
     1321 ggaacggtga gccaactcaa ggagaccttt ggtaagatga agactctctc ttttgaactg
     1381 ctaccaggtc aaagtcatct cgtctctcac tatgaaggtc tgtctgatat gaccattgat
     1441 agacaaggaa acagcctcaa cattgaattt gatagttctc gctaccagtc agctgacatt
     1501 atcaagcaaa ccctgtctga ttttgaaatc cgcgatttga agatggtgga tacggatatt
     1561 gaggatatta tccgtcgctt ctaccgaaag gagctctagg atgatcaaat tgtggagacg
     1621 ttataaaccc tttatcaatg caggggttca ggagttgatt acttaccgag tcaactttat
     1681 tctctatcgg attggcgatg tcatgggggc ttttgtggcc ttttatctct ggaaggctgt
     1741 ctttgattct tcgcaagagt ctttgattca gggcttcagt atggcggata tcaccctcta
     1801 catcatcatg agttttgtga ccaatcttct gactagatcc gattcgtcct ttatgattgg
     1861 ggaggaggtc aaggatggct ccattatcat gcgtttgttg cgaccagtgc attttgcggc
     1921 ctcctatctt ttcaccgagc ttggttccaa gtggttgatt tttatcagcg ttggccttcc
     1981 atttttaagt gtcattgtct tgatgaaaat catatcgggt caaggtattg tagaggtgct
     2041 aggattaact gtcatttatc tttttagctt aacgctcgcc tatctgatta actttttctt
     2101 taatatttgc tttggatttt cagcctttgt gtttaaaaat ctttggggtt ccaacctact
     2161 taagacttcc atagtggctt ttatgtcggg gagtttgatt cccttggcat tctttccaaa
     2221 ggttgtttca gatattctct cctttttgcc tttttcatcc ttgatttata ctccagttat
     2281 gatcattgtt ggaaaatacg atgccagtca gattcttcag gcactccttt tgcagttctt
     2341 ctggctctta gtgatggtgg gattgtctca gttgatttgg aaacgggtcc agtcctttat
     2401 caccattcaa ggaggttagt atgaaaaaat atcaacgaat gcatctgatt tttatcagac
     2461 aatacatcaa acaaatcatg gaatataagg tggattttgt ggttggtgtt ttgggagtct
     2521 ttctggctca aggcttgaat ctcttgtttc tcaatgtcat ctttcaacat attccattcc
     2581 tagaaggctg gacctttcaa gagatagctt tcatttatgg attttccttg attcccaagg
     2641 gaatggacca tctctttttt gacaatctct gggcactagg gcaacgccta gtccgaaaag
     2701 gggagtttga caagtatctg actcgtccca tcaatcctct ctttcacatc ctagttgaaa
     2761 cctttcagat tgatgccttg ggtgaactct tagtcggtgg tattttattg ggaacaacag
     2821 tgaccagcat tgtttggact cttccaaaat tcctgctttt cctagtttgt attccttttg
     2881 cgaccttgat ttatacttct cttaaaatcg caacagccag tatcgccttt tggactaagc
     2941 agtcaggcgc catgatttac atcttctata tgttcaatga ctttgctaag tatccgattt
     3001 ctatttacaa ttctcttctt cgttggttga ttagctttat cgtgcctttc gcctttacag
     3061 cctactatcc agctagctat ttcttacagg aaaaggatgt gttctttaac gtaggaggtt
     3121 tgatgttgat ttctctggtt ttctttgtta tttcccttaa actttgggat aagggcttag
     3181 attcctacga aagtgcgggt tcgtaaaagc taaagtaaga ctaaaatcaa gaaaggaact
     3241 tatgatgttt gtaattgaag aagtcaagga tgaaaatcaa aaaaaggcag ttgtcgctga
     3301 ggttttgaag gatttgccag aatggtttgg aatcccagaa agcacataag cctatataaa
     3361 aggaaccacg acactgcaag tttggaccgc ctatcaggag agtgatttga ctggatttgt
     3421 aagcttatcc tattcgagtg aagattgtgc agagattgat tgtctcggcg taaaaaagct
     3481 tatcaaggta gaaaaattgg gagccaattg cttgctactt tagagagtga agctcataaa
     3541 aaagttggtt atctgcaggt caaaacagtg gcagaaggtt ctaataaaga ttatgatcga
     3601 acaaatgact tttatcgagg tcttggcttt aaaaagttag agatttttct tcaactatgg
     3661 aatccgcaaa atccttgtca gattttgatt aaaaagcttg aataatatta cttgacatct
     3721 attctcagag tgctatactg taagtgtaat cgccgattta gcttagttgg tagagcaagg
     3781 cactcgtaaa gcctaggtta taggtagata aacgactgag gatttgaaaa aatagatagg
     3841 tagaagataa ccgttaagcc ttactcttag cggttattta tattgtttaa tagcgctgat
     3901 atttcatcaa ttatgcctgt tttcgtgttt ctggtagttg ttcaagttta ttgctactat
     3961 ttttgatggt atgaatgtgc ttataatgta tcccggttaa cgaaagtttt ggacttatac
     4021 tcttcgaaaa tctcttcaaa ccacgtcaac gtcgccttgc cgtgcgtatg gttactgact
     4081 tcgtcagttc tatccacaac ctcaaaacag tgttttgagc tgactacgtc agttccatct
     4141 acaacctcaa aacagtgttt tgagcaacct gcggctagtt tcctagttta ctctttgatt
     4201 ttcattgagt actaatctta ttttttaacg tgtgctaaaa aataaattaa tgcatgttaa
     4261 tttttcttga attaaatttt tttaaatgat atactgtttt tatggagaga taacaattat
     4321 atataaatgt actactctat ttcctagata atacttagta aaacttttta taacaaaggc
     4381 tagcaaagtt aaagtttatt atctattgga agttaaataa atatgtaagg aattaaaatg
     4441 aaaaaaagta cagtattgtc attaaccaca gctgcagtta ttttagcagc ctatgcccct
     4501 aatgaggtag tcttagcaga cacatctaac tctgaagatg ctttaaacat ctctgataaa
     4561 gaaaaagtag tattagataa ggaaacagaa aataaagaga aacatgaaga tattcataat
     4621 gctatagaaa cttcacagga ttttaaagag aagaaaacag cagtcattga gggaaaagaa
     4681 gttgttagta aaaatcctgt gacagacaat aacactagca atgaagaagc aagaatcaaa
     4741 gaagattcca atcaatccca aggagatcat acggactcat ttgtgaataa aaacacagaa
     4801 aatcccaaaa aagaagataa agttgtctat attgctgaat ttaaagataa agaatctgga
     4861 gaaaaagcaa tcaagcaact atccaatctt aagaatacaa aagttttata tacttatgat
     4921 agaattttta acggtagtgc catagaaaca actccagata acttggacaa aattaaacaa
     4981 atagaaggta ttacatcggt tgaaagggca caaaaagtcc aacccatgat gaatcatgcc
     5041 agaaaggaaa ttggagttga ggaagctatt gattacctaa agtctatcaa tgctccgttt
     5101 gggaaaaatt ttgatggtag aggtatggtc atttcaaata tcgatactgg aacagattat
     5161 agacataagg ctatgagaat cgatgatgat gccaaagcct caatgagatt taaaaaagaa
     5221 gacttaaaag gaactgataa aaattattgg ttgagtgata aaatccctca tgcgttcaat
     5281 tattataatg gtggcaaaat cactgtagaa aaatatgatg atggaaggga ttattttgac
     5341 ccacatggga tgcatattgc agggattctt gctggaaatg atactgaaca agatatcaaa
     5401 aactttaacg gcatagatgg aattgcacct aatgcacaaa ttttctctta caaaatgtat
     5461 tctgacgcag gatctgggtt tgcgggtgat gaaacaatgt ttcatgctat tgaagattct
     5521 atcaaacaca acgttgatgt tgtttcggta tcatctggtt ttacaggaac aggtcttgta
     5581 ggtgagaaat attggcaagc tattagggca ttaagaaaag caggcattcc aatggttgtc
     5641 gctacgggta actatgcgac ttctgcttca agttcttcat gggatttagt agcaaataat
     5701 catctgaaaa tgaccgacac tggaaatgta acacgaactg cagcacatga ggatgcgata
     5761 gcggtcgctt ctgctaaaaa tcaaacagtt gagtttgata aagttaacat aggtggagaa
     5821 agttttaaat acagaaatat aggggccttt ttcgataaga ataaaatcac aacaaatgaa
     5881 gatggaacaa aagctcctag taaattaaaa tttgtatata taggcaaggg gcaagaccaa
     5941 gatttgatag gtttggatct taggggcaaa attgcagtaa tggatagaat ttatacaaag
     6001 gatttaaaaa atgcttttaa aaaagctatg gataagggtg cacgcgccat tatggttgta
     6061 aatactgtaa attactacaa tagagataat tggacagagc ttccagctat gggatatgaa
     6121 gcggatgaag gtactaaaag tcaagtgttt tcaatttcag gagatgatgg tgtaaagcta
     6181 tggaacatga ttaatcctga taaaaaaact gaagtcaaaa gaaataataa agaagatttt
     6241 aaagataaat tggagcaata ctatccaatt gatatggaaa gttttaattc caacaaaccg
     6301 aatgtaggtg acgaaaaaga gattgacttt aagtttgcac ctgacacaga caaagaactc
     6361 tataaagaag atatcatcgt tccagcagga tccacatctt gggggccaag aatagattta
     6421 cttttaaaac ccgatgtttc agcacctggt aaaaatatta aatctacgct taatgttatt
     6481 aatggcaaat caacttatgg ctatatgtca ggaactagta tggcgactcc aatcgtggca
     6541 gcttctactg ttttgattag accgaaatta aaggaaatgc ttgaaagacc tgtattgaaa
     6601 aatcttaagg gagatgacaa aatagatctt acaagtctta caaaaattgc cctacaaaat
     6661 actgctcgac ctatgatgga tgcaacttct tggaaagaaa aaagtcaata ctttgcatca
     6721 cctagacaac agggagcagg cctaattaat gtggccaatg ctttgagaaa tgaagttgta
     6781 gcaactttca aaaacactga ttctaaaggt ttggtaaatt catatggttc catttctctt
     6841 aaagaaataa aaggtgataa aaaatacttt acaattaagc ttcacaatac atccaacagg
     6901 cctttaacct ttaaagtttc agcatcagcg gtaactacag attctctaac tgacagacta
     6961 aaactggatg aaacatataa agatgaaaaa tctccagatg gtaagcaaat tgttccagaa
     7021 attcatccag aaaaagtcaa aggggcaaat attacatttg agcatgacac tttcactata
     7081 ggggcaaatt ctagctttga tttaaatgcg gttataaatg ttggggaggc taaaaataaa
     7141 aataaatttg tagaatcatt tattcatttt gagtcagtag aagaaatgga agcattaagc
     7201 tccaatggca agaaaaccga cttccaacca tctttatcaa tgcccctaat gggatttgct
     7261 gggaattgga accacgaacc aatccttgat aaatgggctt gggaagaagg atcaaaatca
     7321 aaaacaatgg aaggttatga tgatgacggt aaaccaaaaa ttccaggaac cttaaataag
     7381 gggattggtg gagaacatgg tatagataaa tttaatccag caggagttat ccaaaataga
     7441 aaagataaaa atagaacatc tctagatcaa gatccagact tatttgcctt taacaaccaa
     7501 ggtgttcatg cagaatccac tagtggttct aagattgcta acatttatcc tttagattca
     7561 aatggaaatc ctcaagatgc tcaactagaa agaggactaa ctccatctcc acttgttcta
     7621 agaagtgcag aagaaggact aatttcaatt gtaaacacca acaaagaagg agaaaaccaa
     7681 aaagacctta aagttgtatc aagagagcac ttcataagag gaattctaaa ttctaaggga
     7741 aatgacgcta agggtatcaa atcatccaaa ctaaaagttt ggggtgactt gaaatgggat
     7801 ggactcatct ataacccaag aggtagagaa gaaaatgccc cagaaagtaa ggataaccaa
     7861 gatccagcta ctaagatacg aggacaattt gaaccgattg cggaaggtca atatttctat
     7921 aaatttaaat atagattaac taaagattac ccatggcagg tttcctatat tcctgtaaaa
     7981 attgataaca cagcccctaa gattgtttcg gttgattttt caaatcctga aaaaattaag
     8041 ctaatcacaa aggatactta tcacaaggta aaagatcaat acaagaatga aactttattt
     8101 gctagagatc aaaaagaaca tcctgaaaaa tttgatgaga ttgcaaatga ggtgtggtat
     8161 gcaggagctg ctcttgttaa tgaagatgga gaggttgaga aaaatcttga agtaacttat
     8221 gcaggtgagg gtcaaggaag aaataggaag cttgataaag atgggaatac tatctatgaa
     8281 attagcggag caggagatct aagaggaaaa atcatcgaag taatcgccct agatggagca
     8341 agcaacttta ccaaaatcca taggattaaa tttgctaatc aagctgatga aaaagggatg
     8401 atttcctatt atctagtaga tcctgatcag gattcatcta aataccaaaa gcttggcgaa
     8461 attcctgaat ctaaatttaa gaatttaaaa aatgttaaag atgatagcct aaacaaagag
     8521 acagctgagg tggaaaataa ccttttagta gataatcagt ctatcgaagg aaaatctctc
     8581 tttaatattc ataaaactat ttcaacaatt agagactttg aaaataaaga cctaaagaaa
     8641 ctcattaaaa agaaatataa gcaagaagac gactttgtaa atggtggaac aagaacagta
     8701 gagcgtgatt ataaatacga tgataaagga aatataatag cctacgatga tgggactgat
     8761 ctagaatatg aaactgagaa acttgacgaa atcaaatcaa aaatttatgg tgttctaagt
     8821 ccgtctaaag atggacactt tgaaattctt ggaaagataa gtaatgtttc taaaaatgcc
     8881 aaggtatatt atgggaataa ctataaatct atagaaatca aagcgaccaa gtatgatttc
     8941 cactcaaaaa cgatgacatt tgatctatac gctaatatta atgatattgt ggatggatta
     9001 gcttttgcag gagatatgag attatttgtt aaagataatg atcagaaaaa agctgaaatt
     9061 aaaattagaa tgcctgaaaa aattaaggaa actaaatcag aatatcccta tgtatcaagt
     9121 tatgggaatg tcatagaatt aggggaagga gatctttcaa aaaacaaacc agacaattta
     9181 actaaaatgg aatctggtaa aatctattct gattcagaaa aacaacaata tctgttaaag
     9241 gataatatca ttctaagaaa aggctatgca ctaaaagtga ctacctataa tcctggaaaa
     9301 acggatatgt tagaaggaaa tggagtctat agcaaggaag atatagcaaa aatacaaaag
     9361 gccaatccta atctaagagt cctttcagaa acaacaattt atgctgatag tagaaatgtt
     9421 gaagatggaa gaagtaccca agctgtatta atgtcggctt tggacggctt taacattata
     9481 aggtatcaag tgtttacatt taaaatgaac gataaagggg aagctatcga taaagacgga
     9541 aatcttgtga cagattcttc taaacttgta ttatttggta aggatgataa agaatacact
     9601 ggagaggata agtccaatgt agaagctata aaagaagatg gctccatgtt atttattgat
     9661 accaaaccag taaacctttc aatggataag aactacttta atccatctaa atctaataaa
     9721 atttatgtac gaaatccaga attttattta agaggtaaga tttctgataa gggtggtttt
     9781 aac
//
LOCUS       DAVHIT010000071         9764 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVHIT010000071 DAVHIT010000000
VERSION     DAVHIT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.072"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..319)
                     /locus_tag="VVN16_001607"
     CDS             complement(<1..319)
                     /locus_tag="VVN16_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU5572950.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSP"
     gene            1026..2501
                     /locus_tag="VVN16_001608"
     CDS             1026..2501
                     /locus_tag="VVN16_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5572951.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(2467..3182)
                     /locus_tag="VVN16_001609"
                     /pseudo
     CDS             complement(2467..3182)
                     /locus_tag="VVN16_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3269..3406
                     /locus_tag="VVN16_001610"
     CDS             3269..3406
                     /locus_tag="VVN16_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572952.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            3437..4297
                     /gene="speE"
                     /locus_tag="VVN16_001611"
     CDS             3437..4297
                     /gene="speE"
                     /locus_tag="VVN16_001611"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU5572953.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            4294..5553
                     /locus_tag="VVN16_001612"
     CDS             4294..5553
                     /locus_tag="VVN16_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU5572954.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            5553..6680
                     /gene="nspC"
                     /locus_tag="VVN16_001613"
     CDS             5553..6680
                     /gene="nspC"
                     /locus_tag="VVN16_001613"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU5572955.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6677..7762
                     /gene="aguA"
                     /locus_tag="VVN16_001614"
     CDS             6677..7762
                     /gene="aguA"
                     /locus_tag="VVN16_001614"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU5572956.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLYEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEVLEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGSAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7772..8647
                     /gene="aguB"
                     /locus_tag="VVN16_001615"
     CDS             7772..8647
                     /gene="aguB"
                     /locus_tag="VVN16_001615"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU5572957.1"
                     /translation="MKNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8828..9637)
                     /locus_tag="VVN16_001616"
     CDS             complement(8828..9637)
                     /locus_tag="VVN16_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5572958.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2841 a         1870 c         2181 g         2872 t
ORIGIN      
        1 caggcgaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc ttggtcaagc
       61 ttgatttctt gaccgagcgc ttgcgattgt tttcataaga ctgttgagca tagtcggcag
      121 aataaacctc tttgaagcgc ccttttccaa gacattgtcg gactgtccca cgcttgattt
      181 cagtgtggat agtttgagga gcttttccaa gtagagaggc aatttctcta tttgatttcc
      241 cttctttttt ccatctttcg attaagcgac ggctatcgat tgtcaaatgt ttggcttttg
      301 tagtataatt gtcttgcatc tctgtgcctt tcttgtgttt gtggttgaac aacaagtata
      361 acacagaggt gttttcttat gcctacaaga gctatcggct agttgaacca tctaattttt
      421 aggagggctg ggtggctaac ttcattatag aactttcatt tacgaacata tagtaaaatg
      481 aaacaagaac agaacaaatc gatcaggaca gtaaaatcta tttctaacaa tgttttagaa
      541 gcagaggtgt actattctag tttcaatcta ctatattttt attttttatc aaaaaatact
      601 ttacaagttc ttaaaaacat gatatagtaa taaagcttag aaaatgagat gatgttttct
      661 agcaaatata aacccgagta aaaaatgcct acggacaggc agggttgaat gccgaagcgt
      721 ggttgaaaag ccacattatt gatagggtta aaagcctact tttataagtt gatgttagga
      781 cacttgttct aattcataaa tttttagtgt ggtgaaagca cacgtcatct tgtgaaacga
      841 tcaataaagt acgtaatatt tgctactaga gagttaggaa acatcgggaa cagacatact
      901 caacagaaac caaaataaac acgtcagaag attgcagagc aggtgaaaac ctgctctttt
      961 ttcatgagtc aacctttagt tccttagttt tcataaggtc ctaaaaatat tgaaaggagt
     1021 atgttttgaa agagttagat caaaaccaag ccccaattta tgaggccttg gtgaagttac
     1081 gcaagaaaag gattgttccc tttgatgttc caggtcacaa gcgtggacgg ggaaatccag
     1141 aacttgtcga actcttagga gaaaaatgtg taggcattga tgtcaattcg atgaaaccct
     1201 tggataattt aggccatcct atttcgatta ttcgtgatgc agaggagctg gctgcagatg
     1261 cttttggagc tagccatgcc tttctaatga ttggtggaac aacttcatcg gtgcagacta
     1321 tgattctggc aacctgcaag gcaggagata agattattct gccacgaaat gtccataaat
     1381 ctgctatcaa tgcgttggtt ctatgtggtg ccattcccat ctatatcgag atgagtgtag
     1441 atcctaagat tggtatcgct ttaggtcttg aaaatgaccg agtagcacag gccataaagg
     1501 accatccaga tgctaaggct atcctaatca acaatcctac ttactacggc atctgttcag
     1561 acctaaaggg gttgacagaa atggctcatg aagctggcat gatggtttta gtagatgaag
     1621 cccatggagc gcatttgcat ttcactggta aacttccaat ttctgctatg gatgcaggag
     1681 ctgatatggc agcagtttcc atgcataagt ctggtgggag tttgacacaa agttcgcttc
     1741 ttttaatcgg ggagcagatg aatcccgagt acgttcgtca gattataaac ctgacccagt
     1801 ctacatctgc ctcttactta ttgatggcta gtttggatat ttcacgtcgc aacttggccc
     1861 ttcgtggtaa agagtcgttt gaggaagtca ttgagctatc cgagtacgct cgtcatgaaa
     1921 tcaatgccat tggcggttac tatgcttact caaaagagtt aatagatggt gtgtcggtct
     1981 gtgattttga tgtaaccaag ttgtcagttt acactcaggg aataggctta acaggtatcg
     2041 aggtttatga cctcttgcga gacgaatacg acattcagat tgagtttggt gatatcggca
     2101 atatcttggc ctatatttcc atcggcgacc gcatccaaga catcgagcgc ttggttggtg
     2161 ctctggctga tattaagaga ctctattcac gagatgggaa ggacttaata gctggagaat
     2221 atattcagcc cgagttggtg ctgtctccac aagaagcctt ctattcagag agaaaaagtt
     2281 tgaccttaga tgagtctgtt ggacaggtct gtggagaatt tgtcatgtgc tatcctccag
     2341 ggattcctat cttggctcct ggtgaacgta ttacacgaga aattgtagac tatatccaat
     2401 tcgccaagga acgtggttgc tccctccaag ggacggaaga tccagaggtc aatcatatca
     2461 acgttattaa gagaaagaca aactataaga aaagtcaata gttttatcta aactatttct
     2521 tatttcaatt tgatgatttg gcgatgattt tagagcacgg caaaaagccc ttgaattaga
     2581 agcggtcaat cgcttaattt ctatcagctt atcaaatcct gcctcaatcc ttttctgagg
     2641 attagggtag cgtgtcaaga gttggtaggt atattctgaa tgctttccaa cgattttatc
     2701 caactcagga aagatgatat caagacaacg agtgtattgt actttccaat cagactgttt
     2761 ttcttgagac gatgaatatg tctagccagt atttttaggt ctacttgccg attatcgtgt
     2821 tgaaattgtt cacgattggg gtcagaaaga agtttaagag cgatgccatg agcgtctttc
     2881 ttatccgttt tagtcttgcg aagtgataat gatttggcaa attccttgat gagcaaagga
     2941 ttgtaggtgt aaactttata tccttgttca tgcaggaagt tcagtagatt aaaggcataa
     3001 tgtccagtat cttcaagagc gatgagacag tcttggttga gctgtcgaag agacagatct
     3061 aagagttcaa aaccagcttt attatttgaa aaagtgagtg gtttaagaac agtttttcct
     3121 tgaacattca aggctgtaac atcgtgttta tttttagcga catcaatgcc cacataaagc
     3181 atgggagtac ctccagatgt agtatttcaa gtctacttgg ttatccacga actttttgcc
     3241 ttgttacctt agacgagata aaacgtctat gcgttatcaa actcattacc aattgaaaca
     3301 aaagctgtgg ttagagcctt tcggaaatcg tcaagcgatt ggaggaaatg aactaatcca
     3361 cagtggctta ttccaagtat accacttggg ctttggcagt agctaactgc gctaaatata
     3421 atataaggag aaatagatgg atttatggtt ttctgaagtt catactccag atgtcaaatt
     3481 gtctctgaga acagccaagc aactttacgc tggaaaaagt gaatggcagg atatcgaagt
     3541 cttggatacg ccagcttttg ggaaaatact gattttaaat ggccatgtct tgttctcaga
     3601 tgcggatgat ttcgtctaca atgaaatgac cgttcacgtt cccatggctg tccacccaaa
     3661 tccaaagaaa gtattggtta ttgggggtgg tgacggcggt gttgcccaag tattaacact
     3721 ctatcctgaa ctggaacaaa tcgatatcgt ggaaccggat gagatgttgg tcgaggtctg
     3781 tcgtgagtat ttcccagact ttgctgcagg gctagatgat cctcgtgtta ccatttacta
     3841 ccaaaatggg ctacgctttt tgcgaaactg cgaagatgat tacgatatta tcatcaacga
     3901 tgcgacagat ccatttggcc atacggaagg actctttacc aaggaattct acggcaatag
     3961 ttatcgagct ttgaaggaag acggcatcat gatttaccag catgggagtc ccttctttga
     4021 cgaggatgag tcggcctgcc gaagcatgca ccgcaaggtc aatcaagcct ttccaatcag
     4081 tcgggtttat caggcccata ttccaactag cccagctggc tattggttgt ttggatttgc
     4141 atcgaaaaaa taccaccctg tcaaagattt tgacaaggaa ggttggaaaa aacgccagct
     4201 tttcacagaa tactacactg caaacttaca cgtgggagcc tttatgttgc ccaagtatgt
     4261 tgaggacatt ttagaagaag aggaaggaaa aaaatgagtc gtttactagt tattggttgt
     4321 gggggcgttg cccaagttgc tatttcaaaa atttgtcaag atagcgaaac atttacagag
     4381 attatgattg ctagccgtac caagtcaaaa tgcgatgact tgaaagcgaa gctagaaggc
     4441 aaaacaagta ctaaaattga aactgcagca cttgatgctg acaaggtaga agaggtcatt
     4501 accttgattg aaagctacaa accagaagct gttttgaatg tagctctgcc ttatcaagat
     4561 ttaaccatta tggatgcttg tttggcaaca ggtgttcact atatcgatac agccaactac
     4621 gaagcagaag acacagaaga ccctgagtgg cgtgctatct acgaaaaacg ttgtaaggaa
     4681 cttggtttta cagcctactt tgactactca tggcagtggg cttatcaaga gaaattcaaa
     4741 gaagcaggct tgactgctct tcttggttct ggttttgacc caggtgtaac tagtgtcttt
     4801 tcagcttatg ccctcaaaca ctattttgat gaaatccatt atatcgacat tttagactgt
     4861 aatggcggtg accacggtta tccatttgca accaacttta atccagaaat taatctccgt
     4921 gaggtttctg cgccaggttc ttactgggaa gatgggaaat gggtcgaagt cgaagctatg
     4981 tctatcaagc gtgagtatga tttccctcaa gttggacaaa aagatatgta tctccttcac
     5041 catgaagaaa tcgaatcatt ggccaagaac attccaggtg tcaaacgcat tcgtttcttt
     5101 atgacttttg gtcaatctta cttgacgcac atgaaatgtc ttgaaaatgt tggactcctt
     5161 cgtacggata ccattaactt taacggccaa gaaattgttc caattcaatt tttgaaagcc
     5221 ttgcttccag atcctgctag ccttggccca cgtactgtag gtaaaaccaa tattggatgt
     5281 atctttacag gtgtcaaaga tggcgttgaa aagactatct acatctacaa tgtttgcgac
     5341 catcaagagt gctatgcaga agtcgggtca caagcaattt catacacgac aggcgttcca
     5401 gccatgattg ggacaaaatt agtcatgaac ggaacttgga aacaagctgg agtgtataac
     5461 cttgaggagt tagatccaga tccattcatg gaagctttga atgagtatgg tttgccatgg
     5521 gttgtggttg aaaatccaca aatggtggac taatgaagtt agaacaagta ccaacaccag
     5581 cctatgttat tgacttggcc aagttagaag ctaattgccg cattctacaa tatgtacaag
     5641 aagaggtcgg ttgcaaggtc ttgcttgccc agaaggcata ttccctctac aaaacttatc
     5701 ccttgattag ccagtatcta tcaggtacga cagctagtgg actctatgag gccaaattgg
     5761 caagggaaga atttcctggt gaagtccatg tatttgcgcc tgctttcaag gatgcagact
     5821 tggaggaatt gcttgggata acagaccata tcgtctttaa ctcagagaga cagttgcgta
     5881 agcatggggc tcgttgtcga gaggctggtg tcagtgttgg tttgcgcctc aaccctcagt
     5941 gttcaactca aggagatcac gcgctctatg acccttgtgc acctggttct cgctttggag
     6001 ttactataga caagattccg agtgatttgc tagatttggt tgacggactt cattttcata
     6061 ccctttgcga gcagggagca gatgatttac aaacaacttt gaaagcagta gaagaacagt
     6121 ttggtcccta cttacatgag gtaaaatggc tcaatatggg tggtggtcat catattacaa
     6181 gagaaggtta cgatgtggat ttgctgattt cagaaatcaa gcgtatccga aaaacttaca
     6241 atcttgaaat ctatatcgag cctggtgaag ccattgcgct taatgcgggt tatttagcaa
     6301 ctgaggtatt agatattgta gaaaacggta tggaaatctt ggttttagac gcctctgcga
     6361 cctgccatat gcctgatgta cttgagatgc cctatcgtcc acctttgaga aatggctttg
     6421 agtcacagga aaaagcccat acctacagac tttcttctaa tacctgtctg acgggcgatg
     6481 tgattggtga ttatagtttt gaaaatccag tccaaatcgg agacagactt tattttcaag
     6541 acatggccat ttattctttt gtcaaaaata atacctttaa tggtattgga ttgccaagtc
     6601 tctatctcat ggacgaacag ggagactgta gcttactcaa agcttttggc tatcaagact
     6661 ttaaagggag attatcatga tggacagtcc aaaaaaatta ggctatcaca tgccagcaga
     6721 gtacgaaccc catcatggta ccctcatgat atggccgact cgaccaggat catggccttt
     6781 tcaaggaaag gctgctaaaa gagcatttac tcagatcatc gagaccatag cagaagggga
     6841 aagagtctat cttttggtgg agcaggccta tctatatgaa gcccaatcct atcttggaga
     6901 caaggttgtt tatttagaca ttcccaccaa tgatgcctgg gcgcgtgata ctggcccaac
     6961 cattctcgtc aatgataaag gtaagaaatt agccgtggat tgggccttca atgcttgggg
     7021 aggcacctat gatggtcttt atcaagatta tgaagaggat gaccaagtag ccagtcgttt
     7081 tgctgaggtc ttggaaaagc ctgtctatga tgctaaacct tttgtactgg aaggaggcgc
     7141 aatccatagc gatggtcaag gaactattct cgtaactgaa agttgcttgc ttagtcctgg
     7201 tcgcaatcct aacctgacta aagaggagat tgaaaacaca ttattagaaa gtcttggtgc
     7261 tgaaaaagtt atttggcttc cttatggtat ttatcaggat gaaaccaatg aacacgtcga
     7321 taatgttgct gcctttgttg gttctgctga gcttgttttg gcttggacag atgacgaaaa
     7381 tgatccccag tatgccatgt caaaagcaga tctcgaactc ttagaacagg aaacagatgc
     7441 aaaaggttgt cacttcacca ttcataaatt gcctatccct gcagttcgac aagttgtgac
     7501 agaagaagat ttgccaggct acatctatga agaaggagaa gaagagcgat acgcaggtga
     7561 acgactagca gcttcctacg taaactttta tatcgccaac aaggctgtct tggttccaca
     7621 gtttgaggat gtaaacgacc aagtggccat agatatcctc agcaagtgtt tcccagaccg
     7681 taaagttgtc ggaataccag ccagagatat tctcttaggt ggtggcaata tccactgtat
     7741 cacccaacaa attccagaat aggagaaaaa gatgaaaaat gtaagagttg caaccattca
     7801 gatgcaatgc gctaaggatg tggcaacaaa tatccaaacc gcagagcgtt tagtacgtca
     7861 ggctgctgag caaggagccc aaattattct cttgcccgag ttgtttgaac atccctattt
     7921 ctgtcaggaa cgtcagtatg actactacca gtatgcccaa tctgtagcgg aaaatactgc
     7981 aattcagcat tttaaggtga ttgctaagga actacaagtt gttttaccaa tcagtttcta
     8041 tgaaaaagat ggtaatgtct tgtataactc tattgccgtc attgatgcag atggggaagt
     8101 gctgggcgtt tatcgaaaga cccacatacc agatgaccat tattatcaag aaaaattcta
     8161 tttcacgcct ggtaacactg gtttcaaggt ctggaatact cgctatgcta agattggtat
     8221 cggtatctgt tgggatcaat ggttccctga aacagcgcgc tgtcttgcat tgaatggtgc
     8281 tgaattgctc ttttatccta cagctatcgg ttcagagcca attttggata cagatagttg
     8341 tggtcactgg caacgtacta tgcaagggca cgcagcagcg aatattgttc cagtcatcgc
     8401 agccaatcgt tatggtttag aggaggttac tcctagtgag gaaaatggcg gacagagttc
     8461 cagtcttgac ttctacggtt cctcctttat gacggatgaa acaggagcta ttctagaacg
     8521 agctgaaaga caagaagaag ctgttctgtt agctacttat gacctagaca agggagcaag
     8581 tgaacgccta aactggggct tgtttcgaga tagaagacca gaaatgtata gacaaattac
     8641 agattagtgt gggagaaatg agagattcat tctgctagac taacttctta ttagcaacta
     8701 taagatacta tggcatctag taaatcgatt tttatgattc gctattcttg tctattgatt
     8761 agtccgtatt ttaaaatatt agcaaaaaag caaatagcag taacttctgt ctatttgctt
     8821 ttctttttta tagaatatat ttctcaatag cacgcgcaac gccgtcttct tcgttgcttg
     8881 aggtaacggc atccgcaaga gatttgacat aatcgctggc atttcccatt gcaatcccaa
     8941 gccctgcaaa ctggagcatt tcgatatcgt tattagcatc gcccatggcc ataatctctg
     9001 aggaatcaat cttcaaaatc tcagctagtc gtgaaagagc agtagccttt gtcgttccaa
     9061 gcggcattgc ttcataaatg acaggctgcg aacgaactcc actgaatcgt tggcaaagct
     9121 cttcagcaaa acgctgctca aaatcgtctg tttgttcttt tgttcctaaa aacatacctt
     9181 ggaacatccg gaactttcca ctagtcgctt cttcaagaga aatttcagtc aggtctgaaa
     9241 atactagttt agcatcattt tcaataactt gattgggttt gccaccgaga acaaaataat
     9301 gtgactcgtc aaaaagtgtc aactgaacat cactcttttc agcaaggtca tagaggtatt
     9361 cgatgtcagc tggactcagt tctttccagt caactagact ccaatcactg gtctggtgag
     9421 ttgaacaacc gttgttaaca ataatatatt cgttctggag gtcaagctcc agttttttgt
     9481 agtaggggag gacaccgaaa agggggcgac ccgtacagag aaccagtttg acaccttttt
     9541 caatggcttt gtgaatagca gtaatgtgag cttgtgggat ttccttggct tcattgagga
     9601 gggtgccgtc catatccaag gctagtagtt taatcatagg tcttccttct ttatcttttg
     9661 ctattattat agcatatttt tgagaagaaa ttgatagaaa gcttgagact aattgatttt
     9721 atagtttaag atgttttgat gacaattcat gatttgaaga ggat
//
LOCUS       DAVHIT010000072         9754 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVHIT010000072 DAVHIT010000000
VERSION     DAVHIT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.073"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            2..865
                     /locus_tag="VVN16_001617"
     CDS             2..865
                     /locus_tag="VVN16_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5572959.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDNLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLYNFY
                     SLVGREQEYEANYSHLMELYQTKNFEHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1248..1868
                     /locus_tag="VVN16_001618"
     CDS             1248..1868
                     /locus_tag="VVN16_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572960.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1954..2397
                     /gene="tsaE"
                     /locus_tag="VVN16_001619"
     CDS             1954..2397
                     /gene="tsaE"
                     /locus_tag="VVN16_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5572961.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2387..2905
                     /locus_tag="VVN16_001620"
     CDS             2387..2905
                     /locus_tag="VVN16_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5572962.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2913..3929
                     /locus_tag="VVN16_001621"
     CDS             2913..3929
                     /locus_tag="VVN16_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5572963.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4013..5269
                     /locus_tag="VVN16_001622"
     CDS             4013..5269
                     /locus_tag="VVN16_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU5572964.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5324..6490
                     /gene="recA"
                     /locus_tag="VVN16_001623"
     CDS             5324..6490
                     /gene="recA"
                     /locus_tag="VVN16_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU5572965.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6797..8167
                     /locus_tag="VVN16_001624"
     CDS             6797..8167
                     /locus_tag="VVN16_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5572966.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIILQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQSLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8543..9499
                     /gene="lytA"
                     /locus_tag="VVN16_001625"
     CDS             8543..9499
                     /gene="lytA"
                     /locus_tag="VVN16_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU5572967.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNSHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
BASE COUNT         2985 a         1664 c         2165 g         2940 t
ORIGIN      
        1 aatgaataca ctcgcagaga aattcagatt gaaaagaaaa gagttgagac tctcccaaca
       61 aactcttgca gaaggaattt gtgaacaaag tcagattagt aaaattgaga gagggcattt
      121 cattccctcc gcagaccttt tgttcaaact ctcacaacga cttgaagtac cattagatta
      181 tttttttaat gaacaaattg aaattaaatc taacctctct aatttcaagc aattgtctgc
      241 tcgactatta gatgacagaa attatgataa tttggaatat atttatagaa ttgagattga
      301 acgaagtact tttctaacac tagaagaccg aacttatctt gaatggatta aagctattat
      361 tgacttctat caatatgaca gtaagtgtga ggcaatttct tcattggaaa atatattatt
      421 aaaagtctct tcaaatactc tgatttattt aaaggcattg aatactctat ataatttcta
      481 ttccttagtg ggtcgtgaac aagaatatga ggcaaactac tctcatttga tggagttata
      541 tcagacaaaa aattttgagc atcaagagtt tttatttggc tacatcagag ttcgttacaa
      601 ctacgctcac tacctagtat caaaggaaaa atataatgaa gccatccaag aagctcttga
      661 aacgattgaa ctctgtaaac aaagacagac aagctaccaa ttggctcctc tacttattct
      721 tgtaggaaat gctggagcca aatttctaga cagagaacaa gtcaaaaatt attatataga
      781 agcaagagag ctatgcaaga tttataacaa tcctttaatg ctgatgaaga tagaaaatta
      841 tttgaaggaa ctagatactg tttagttaat cttgacatta tagtttttct gaaacgttaa
      901 ataacctgta aggctgatag ttagattaat actatcagcc ttataattat gtgactctga
      961 gtccgtctcg ctccgtgagg tgcgagacaa aacaaccacc cgtttgacgg attttcatga
     1021 tttcaaatct atattcttcg aaaatctctt caaatcacgt cagctctatc tgcaatctca
     1081 aaacactgtt ttgagcaacc tgtggctagc tacctagttt gatctttgat tttcattgag
     1141 tattagtctc tagtgtttgt ttagcgtctt aaagagacat ttgagaagat tagaaacagt
     1201 tctctctttt ctcctagtat aatgattggt aggaagggag agagaagatg aaatcaatga
     1261 gaatcttatt tttgttagct ttaattcaaa tcagtttgag tagctgtttc ctatggaagg
     1321 aatgcatctt gtcctttaaa caaagtacag cttttttcat cggaagcatg gttttcgttt
     1381 caggaatctg tgctggagta aattatcttt atacccgtaa gcaagaagtc catagtgtcc
     1441 tagccagtaa gaagtcggtg aagctttttt acagtatgtt actcttaatt aatttgttag
     1501 gagctgttct tgttttgtca gataacttgt tcatcaaaaa tacgctgcag caagaattag
     1561 ttgacttttt attgccatcc ttctttttcc tatttgggct agatttgctg atttttttac
     1621 ccttgaaaaa atacgtgcgc gattttcttg ctatgctaga cagaaaaaag acagtgttgg
     1681 tgactatttt agcaacactt cttttcttaa gaaatccaat gaccattgtc tcacttctga
     1741 tttatattgg actgggcttg ttttttgcag cctatcttgt cccaaattcg gttaagaagg
     1801 aagtttcctt ttatggtcat attttccgag atcttgtatt ggtcattgtt acgctcattt
     1861 tcttttagag aaagcctgtt ttttatggat gttatgatga taagtttagt aggaaaatag
     1921 acacaaaaga aaagtttttg gtataataag agtatgtaca caaaaaatga agaagagttg
     1981 caagccttag gggagcgttt gggccatcta ttagcaaaga atgatgtttt aatcttaact
     2041 ggagaactgg gtgcaggtaa aacgaccttt actaaaggac ttgcaaaagg attacagatt
     2101 tctcaaatga ttaaaagtcc cacctatact atcgtgagag agtatgaagg tcgacttcca
     2161 ctttatcacc tagatgttta tcgtattgaa ggagatgctg attctatcga cttggatgag
     2221 tttatctttg gtggtggcgt gactgttatt gagtggggaa atctcttagg agatgccttg
     2281 ccagatgctt atttagaatt ggaaattcta aaagaagcag atggacgccg tttaaatttt
     2341 caggcaaagg gtttgcgtgc tgagaaattg ttagaggagc ttcaatatgg agtatgaatt
     2401 gctcattagg gaagcagagc ccaaagatgc agctgaatta gtggcctttt taaatcgtgt
     2461 gagtttggag acagacttta ccagcctaga cggagatggt attctcttga ctagtgagga
     2521 gatggaaata ttcctcaaca agcaagctag ttcggacaat cagataacct tacttgcatt
     2581 cttaaatggc aaaattgctg gtattgtaaa tattacagct gatcagcgta agagagtccg
     2641 tcatattgga gatctcttca ttgtgattgg aaaaagatat tggaataatg gcttgggaag
     2701 tttgttgcta gaagaagcga tagagtgggc acaagcaagt ggcattctgc gtcgtctcca
     2761 actgactgtc caaactcgta atcaagcagc agtccatctt tatcaaaagc atggctttgt
     2821 cattgaaggt agccaagagc gtggggcata tatagaagaa gggaaattta tcgatgttta
     2881 cttgatgggt aaactgatag gttagtagaa atatggttaa aaaaattatt ggaatggtgc
     2941 tagctttact ttctgtaact gtagtaggag taggtgtttt tgcttatact atttatcaac
     3001 aagggacaga aaccttagct aaaacctata aaaaaatcgg cgaagaaacc aaggttattg
     3061 aagcgactga acctctaacc attctgttaa tgggagtgga caccggaaat gttgaacgaa
     3121 ctgaaacttg ggtcggtaga agtgatagca tgatcttgat gacagtgaat cctaaaacga
     3181 aaaaaacaac aatgatgagt ttagagcggg atattctgac gcgcattgaa tcagggaatg
     3241 gtcaggctca tgaagcgaaa ctgaactcag catatgcaga tggtggagca gagcttgcta
     3301 tagaaaccat tcaaaaaatg atgaatatcc atattgatcg ctatgtgatg gtcaatatga
     3361 gagggttgca aaaactagtg gatgcagtag gaggtattac agtcaataat atcctaggtt
     3421 tcccaatttc tatcagtgac caagaagaat ttaatactat ttctatcggt gttggggagc
     3481 aacatattgg gggagaagaa gccctagtct atgcacgaat gcgttaccaa gatcctgagg
     3541 gggattatgg tcgtcaaaaa cgtcaacgtg aagttattca aaaagtcatg gaaaaagctc
     3601 tcagtttaaa tagcattggt cattatcaag agattctaaa agctttgagt gacaatatgc
     3661 agaccaatat tgatttgtct gcaaaaagta tccctaactt gctaggctat aaagattcat
     3721 ttaaaaccat tgaaactcag cagttgcagg gtgaaggaga gatacttcaa ggtgtttctt
     3781 accagattgt ttcgagagca catatgttgg aaatgcaaaa tatactccga cgttctttgg
     3841 gacaagaaga agttactcag cttgaaacca atgcggtttt atttgaagat ttatttggca
     3901 gagcacctgt tggtgatgaa gataattaac ttttgataca aataaaaaat caatcgtagg
     3961 aaaattcctg cgattttttc aaaaaaatac gaatagatag gtaggaggaa acatgaaagc
     4021 agaaatcatt gctgttggaa cagagatttt gacaggacag attgtcaaca ccaatgccca
     4081 gtttttgtcg gaaaaactag ctgagattgg ggtagatgta tattttcaga cggctgtagg
     4141 agacaatgaa gttcgtctct tgtctttgct tgagattgcc agtcaacgta gcagtctggt
     4201 gattttgaca ggcggtttgg ggccaactga ggacgaccta accaaacaaa ccctagctaa
     4261 atttttaggg aaagcattag tctttgatcc tcaggctcag gagaagttgg atatcttttt
     4321 tgccctgcga ccagactatg cccgaacacc gaataacgaa agacaagctc aaattgtaga
     4381 aggagcgatt ccactgccaa acgaaacagg actggctgtt ggaggaaaat tagaagtaga
     4441 cggagtgacc tatgtcgtcc ttccaggtcc gccaagtgaa ttgaaaccca tggtcttaaa
     4501 ccaacttcta cccaagttga tgacagggag caagctgtat tcccgagttc ttcgtttctt
     4561 tgggattggc gagagccagt tggttacgat tttggctgat ttaattgata atcagatcga
     4621 tcctaccttg gccccttatg ccaagacagg agaagtcact ctacgtctgt caacaaaggc
     4681 tagcagtcaa gaagaggcga atcaagcgct ggatatcttg gaaaatcaaa tcttggactg
     4741 ccagactttc gaaggaattt ctttacgaga cttttgctat ggttatgggg aagaaactag
     4801 tttagccagc attgtggtag aagaactgaa aaggcaaggg aaaaccatcg cggctgcaga
     4861 gagtttgacg gcaggtcttt tccaagctac cgtggcgaat ttttctggag tttcaagtat
     4921 atttaagggt ggttttgtga cctatagctt ggaggaaaaa tcaaggatgt tggatattcc
     4981 tgccaagaat ttggaagaac atggtgtggt gtctgaattt acagctcaga agatggctga
     5041 gcaggcacga agcaagaccc agtctgattt tggaattagt ttgactggag tggcaggacc
     5101 agatagccta gaaggacacc cagttgggac agtcttcata ggcttggcgc aagatcaagg
     5161 aactgaggtt atcaaggtta atattggagg cagaagccga gcagatgtac gtcacattgc
     5221 ggttatgcat gcctttaacc tagttcgcaa ggctttatta agtgactaac ttttgatata
     5281 atagtagata ggtctgagga tcattagaat gtaggagaat agaatggcga aaaaaccaaa
     5341 aaaattagaa gaaatttcaa aaaaatttgg ggcagaacgt gaaaaggcct tgaatgacgc
     5401 tcttaaattg attgagaaag actttggtaa aggatcaatc atgcgtttgg gtgaacgtgc
     5461 ggagcaaaag gtgcaagtga tgagctcagg ttctttagct cttgacattg cccttggctc
     5521 aggtggttat cctaagggac gtatcatcga aatctatggc ccagagtcat ctggtaagac
     5581 aacggttgcc cttcatgcag ttgcacaagc gcaaaaagaa ggtgggattg ctgcctttat
     5641 cgatgcggaa catgcccttg atccagctta tgctgcggcc cttggtgtca atattgacga
     5701 attgctcttg tctcaaccag actcaggaga gcaaggtctt gagattgcgg gaaaattgat
     5761 tgactcaggt gcagttgatc ttgtcgtagt cgactcagtt gctgcccttg ttcctcgtgc
     5821 ggaaattgat ggagatatcg gagatagcca tgttggtttg caggctcgta tgatgagcca
     5881 ggccatgcgt aaacttggcg cctctatcaa taaaaccaaa acaattgcca tttttatcaa
     5941 ccaattgcgt gaaaaagttg gagtgatgtt tggaaatcca gaaacaacac cgggcggacg
     6001 tgctttgaaa ttctatgctt cagtccgctt ggatgttcgt ggtaatacac aaattaaggg
     6061 aactggtgac caaaaagaaa ccaatgtcgg taaagaaact aagattaagg ttgtaaaaaa
     6121 taaggtagct ccaccgttta aggaagccgt agttgaaatt atgtacggag aaggaatttc
     6181 taagactggt gagcttttga agattgcaag cgatttggat attatcaaaa aagcaggggc
     6241 ttggtattct tacaaagatg aaaaaattgg gcaaggttct gagaatgcta agaaatactt
     6301 ggcagagcac ccagaaatct ttgatgaaat tgataagcaa gtccgttcta aatttggctt
     6361 gattgatgga gaagaagttt cagaacaaga tactgaaaac aaaaaagatg agccaaagaa
     6421 agaagaagca gtgaatgaag aagttacgct tgacttaggc gatgaacttg aaatcgaaat
     6481 tgaagaataa gctgttaaag cagtggaaaa atccgctact ttttcgattt ttgattcaag
     6541 tttttagatt atatatagta gcttgaaata agatatgaac aactttatta ggaaagtcaa
     6601 attaatttct agaaatgttt tagcagctac agcgtactat tccaaactca accaactata
     6661 atagatcgaa actagaatag tacacatcta cttctaaaac attgttaaaa atcgatttga
     6721 ctgtccttat ttcattccgc tatatataag tttgctgttt cttgtcgctc ctctggaaag
     6781 ctgatataat agctttatga ataaaaaacg aacagtggac ctgatacatg gtccgattct
     6841 tccctcgctc ttaagcttca cctttccaat cttgctatca aatatttttc aacagctcta
     6901 taacactgct gatgtcttga ttgttggacg atttcttggt caagaatcct tggctgcagt
     6961 aggagcgacg acagcgattt ttgacctgat tgtaggtttt acacttggtg ttggcaatgg
     7021 catggggatt gtcattgctc gttattatgg ggctcggaat ttcactaaaa tcaaggaagc
     7081 agtagcagcc acttggattt taggtgctct tttgagcatt ctagttatgt tgctgggctt
     7141 tcttggcttg tatcctctct tgcaatactt agatactcct gcagaaattc ttcctcaatc
     7201 ttatcaatat atttctatga ttgtgacctg tgtaggtgtc agctttgctt ataatctttt
     7261 tgcaggcttg ttgcggtcta ttggtgacag tctagcagcc ctgggatttc tgattttctc
     7321 tgccttggtt aatgtggttc tggatcttta ttttattacg caattgcatc tgggagttca
     7381 atccgcagga cttgctacca ttattttgca aggtttatca gcggttctct gcttttatta
     7441 tattcgtaaa agtgtgccag aactcttgcc acagtttaaa catttcaaat gggacaaaag
     7501 cttgtacgcg gatctcttgg agcaaggttt ggctatgggc ttgatgagtt caattgtatc
     7561 tatcggcagt gtgattttac agttttctgt taatacattt ggtgcagtga ttattagtgc
     7621 ccagacggca gctcgacgca ttatgacctt tgcccttctt cctatgaccg ctatttctgc
     7681 atcaatgacg acctttgctt ctcagagtct aggagctaag cgacctgacc gcattgttca
     7741 aggtcttcga atcggcagtc gtttaagtat atcctgggca gtttttgttt gtattttcct
     7801 cttttttgcc agtccagcct tggtttcctt cttggctagt tcgacagatg gttacttgat
     7861 agaaaatgga agtctctatc tgcaaatcag ttcagccttt tatcccattt tgagcctctt
     7921 gttgatttat cgcaattgct tgcagggctt ggggcagaag atccttcctc tagtttctag
     7981 ctttattgaa ctaatcggaa aaatcgtttt tgtggttttg attattcctt gggcaggata
     8041 taagggtgtt atcctttgtg aacctcttat ctgggttgcc atgacagttc aactgtactt
     8101 ctcactattc cgtcatccct tgataaaaga aggaaaggca attttggcaa ccaaagtgca
     8161 atcctagttg gatttactga ataaaattca tttcctctag tgaacatcga aaaaacttgt
     8221 gttctcttct ttagtttggt gttgaaaata gtttaacaga cttttgactt cttttatatg
     8281 atataataaa gtatagtatt tatgaaaagg acatatagag actgtaaaaa tatacttttg
     8341 aaaagctttt tagtctgggg tgttattgta gatagaatgc agaccttgtc agtcctattt
     8401 acagtgtcaa aatagtgcgt tttgaggttc tatctacaag cctaatcgtg actaagattg
     8461 tcttctttgt aaggtagaaa taaaggagtt tctggttctg gattgtaaaa aatgagttgt
     8521 tttaattgat aaggagtaga atatggaaat taatgtgagt aaattaagaa cagatttgcc
     8581 tcaagtcggc gtgcaaccat ataggcaagt acacgcacac tcaactggga attcgcattc
     8641 aaccgtacag aatgaagcgg actatcactg gcggaaagac ccagaattag gttttttctc
     8701 gcacattgtt gggaacggtt gcatcatgca ggtaggacct gttgataatg gtgcctggga
     8761 cgttgggggc ggttggaatg ctgagaccta tgcagcggtt gaactgattg aaagccattc
     8821 aaccaaagaa gagttcatga cggactaccg cctttatatc gaactcttac gcaatctagc
     8881 agatgaagca ggtttgccga aaacgcttga tacagggagt ttagctggaa ttaaaacgca
     8941 cgagtattgc acgaataacc aaccaaacaa ccactcagac cacgttgacc cttatccata
     9001 tcttgctaaa tggggcatta gccgtgagca gtttaagcat gatattgaga acggcttgac
     9061 gattgaaaca ggctggcaga agaatgacac tggctactgg tacgtacatt cagacggctc
     9121 ttatccaaaa gacaagtttg agaaaatcaa tggcacttgg tactactttg acagttcagg
     9181 ctatatgctt gcagaccgct ggaggaagca cacagacggc aactggtact ggttcgacaa
     9241 ctcaggcgaa atggctacag gctggaagaa aatcgctgat aagtggtact atttcaacga
     9301 agaaggtgcc atgaagacag gctgggtcaa gtacaaggac acttggtact acttagacgc
     9361 taaagaaggc gccatggtat caaatgcctt tatccagtca gcggacggaa caggctggta
     9421 ctacctcaaa ccagacggaa cactggcaga caagccagaa ttcacagtag agccagatgg
     9481 cttgattaca gtaaaataat aatggaatat ctttcaaatc agaacagcgc atattattag
     9541 gtcttgaaaa agcttaatag tatgcgtttt cttgtggaga tatttccttc aatttttgtt
     9601 actatattaa acaaaaatca aaaagcaaac tagaaagtta tgctcaaata aaatctaaat
     9661 ttgacaatgt aaaccgagtc ggatagcttt aagtactgtt ttgaggttga agatacgatt
     9721 tttgatagga actcatcaat tttagatttt taag
//
LOCUS       DAVHIT010000073         9707 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVHIT010000073 DAVHIT010000000
VERSION     DAVHIT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.074"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..101
                     /locus_tag="VVN16_001626"
                     /pseudo
     CDS             1..101
                     /locus_tag="VVN16_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            90..626
                     /locus_tag="VVN16_001627"
     CDS             90..626
                     /locus_tag="VVN16_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5572968.1"
                     /translation="MFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGG
                     NGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFL
                     LELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVALVDKSQLAIQK
                     SLEELETLKKSLMQEYFG"
     gene            686..922
                     /locus_tag="VVN16_001628"
     CDS             686..922
                     /locus_tag="VVN16_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU5572969.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            919..1332
                     /locus_tag="VVN16_001629"
     CDS             919..1332
                     /locus_tag="VVN16_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU5572970.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1450..2415
                     /locus_tag="VVN16_001630"
     CDS             1450..2415
                     /locus_tag="VVN16_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5572971.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRWIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            2438..3019
                     /locus_tag="VVN16_001631"
     CDS             2438..3019
                     /locus_tag="VVN16_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5572972.1"
                     /translation="MFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTK
                     NVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRI
                     NSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQ
                     NEFADFVVQIDKSQFACEIAIKVWRNSLKFSII"
     gene            3049..6399
                     /locus_tag="VVN16_001632"
     CDS             3049..6399
                     /locus_tag="VVN16_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5572973.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIEEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(6904..7374)
                     /locus_tag="VVN16_001633"
     CDS             complement(6904..7374)
                     /locus_tag="VVN16_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5572974.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(7391..9664)
                     /locus_tag="VVN16_001634"
     CDS             complement(7391..9664)
                     /locus_tag="VVN16_001634"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU5572975.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         3132 a         1582 c         2016 g         2977 t
ORIGIN      
        1 aaaaactaaa tacgataagt caaatttatg attttcgtaa aattcaatca gaaaaattta
       61 acgagctcgt caaatcccga tttaacgaga tgtttgggga tcctttaaat aataacaaga
      121 aatttgcagt aaaaacggga caacagtgtt ttaaattttc aagtggaaaa tttcttgata
      181 aacatgatag agtttttgaa ggttatcctg cctatggtgg aaatggcatt gcttggaaaa
      241 gtcgaaaata tttaattgat aatcctacta tcatcattgg tcgtgtaggt gcatattgtg
      301 gtaatgtaag aactacccac ggtaaggtat ggataagtga caatgctata tatataaaag
      361 aatttaagaa ttccgatttc aatcttgtat ttttattaga attaatgaaa gtaattgatt
      421 ttagtaaatt tgctgatttc tcaggtcaac caaagataac tcaaaaacca ttagaaaatc
      481 agaaatatat cctcccccct ctagccctcc aaaatgagtt cgcagacttt gtagccctgg
      541 tcgacaaatc acaattggca atccaaaaat ctctggaaga acttgaaact ttgaagaaat
      601 ctctgatgca ggagtatttt ggctgatatt ctgccattgt aattacggta atgatttgtt
      661 ataatacttc aaaggaggaa atcagatggt agtaaaaaca agaaaacaag gaaattcaat
      721 caccattacg attccaagtg aatttaatat tccaagtggt gttaaatacg aagcgaaatt
      781 gttaccaagt ggtgagatta tctttactcc tgaagaattg gggcagcagg tttcttatgt
      841 atctgatgat gcctttgact taaatttaga taaaatattt gacgaatacg acgatgtttt
      901 caaagctttg gtggaaaaat gacaatctat ctgacagaaa agcaaattga aaaaataaat
      961 gctttagcaa ttcaacggta ttctccaaat gagaaaattc aaacagttag ttcttctgcc
     1021 ttaaatatga ttgtgaactt accagaacaa tttgtctttg ggaagtctct ttatccaaca
     1081 atttttgata aagcaacgat actatttgtc caattgataa agaagcatgt ttttgctaat
     1141 gctaataaaa gaactgcttt cttcgttttg gtcaaatttt tacaattaaa cggctatcgt
     1201 ttttctgtaa cggtagaaga agcagtaaaa atgtgtgtaa ccatcgcagt agaagcttta
     1261 actgatgaaa aaatgacaag ctactccaaa tgggtttctg aacattctgt tagagaaaag
     1321 gtcaaaaagt aacctagtat gctggatttg aatgagcaca agaaaataaa tgaacagaca
     1381 atattagaat tctgtaatgc agaaactgat attgtctctt tttattgatg aataagaaag
     1441 tgagaaatta tggaatcaaa agttacaatt atcatgcaag aaatgttacc tcttttaaat
     1501 aatgaacaat tactagcgtt gagagagagt ttagaacatc atctagtaga cggaaaaaag
     1561 cagcagaagt attcgaataa taacctgttg caactattta ttaccgccaa gcaggtagag
     1621 ggctgtagct caaaaacaat tcgttattac cagaggacga ttgaaaactt gtttaatgct
     1681 attaaagagt ctgtgacaca actcacaaca gatgatttaa ggagttattt agcaaattac
     1741 cagtctgaaa aggattgtag taaggcaaat ttagacaata ttaggcgtat attgtcttct
     1801 ttttttgctt ggcttgagca agaggaatat atcattaaaa atcccattcg atggataaag
     1861 aaaattaaga ctgagcaaaa tgtgaaggaa acttatactg atgaacattt ggaaattatg
     1921 cgtgataact gtgaaaattt gagagatttg gcaataatag acctactagc atcgacaggt
     1981 atgcgtgtag gggagcttgt acagttgaat cgttcagata ttgattttga aaacagagag
     2041 tgtgttgtct ttggtaaagg aaagaaggag agaccagtat attttgacgc tcggacgaaa
     2101 attcatttaa gaaattatct taacgacaga aaagatagtc accctgctct ttttgtaacg
     2161 ctagttggaa aagcccagag acttggaatt gctggtgtag agattcgctt aagaaagtta
     2221 ggagacaaac tcggcataca aaaggttcac ccacataagt tcagaagaac tttagcgact
     2281 aaggcaattg ataaaggtat gcctatcgaa caagtccaaa aactgctagg tcatagcaag
     2341 attgacacaa ccctggccta tgccatggtc aatcaaaata atgtcaagca ttcacaccaa
     2401 aaattcatct cttaaaagca aatcccgatt taacgagatg tttggggaaa ataaaatatt
     2461 tgaaagcatt gataacttat ttgatattat agatggtgat aggggcaaaa attatcctaa
     2521 atcagatgag ttgtttagtg aggagtactg tttattttta aatacaaaga atgttactaa
     2581 aaacggattt tcattcgata caaagcaatt tatcactaaa acaaaggata aattacttcg
     2641 aaaaggcaaa cttgagcgtt atgatatagt cttgacaaca agaggtactg ttggaaatgt
     2701 agcgtactac gatgaattaa taaaatataa acatttacgt ataaattcag gtatggtaat
     2761 attacgtccc aagacaccaa atctaaatca gaaatttatt atccatgttt taaggaataa
     2821 taattatagt cgagtgatat caggaagtgc tcagcctcag ttaccaatta caaaattaaa
     2881 aaaaatactt ctccccctcc ccccactagc cctccaaaat gagttcgcag actttgtagt
     2941 tcagatcgac aaatcacaat ttgcttgtga gatagctata aaagtgtgga gaaatagctt
     3001 gaaatttagt ataatatagc taaactattt gtttaaagtg agaaaaaaat gggaaatttt
     3061 agctttcttt taaaaaatga cgaatatgaa tctttttcaa aaccttgcat tgaagctgag
     3121 aatatgattg ctacatcaac tgtggctact gcctttatgg cgcgtcgtgc tttagagcag
     3181 gctgtccatt ggatatatag tcacgattca tatttagaag ctccctatcg tgctactcta
     3241 tcttctttag tatgggatga tgattttagg gatatcgtag attctgaact ccacaagcag
     3301 atagttctgt tgattcggtg gggaaaccat gctgctcatg gtggtgaaat taaggaacga
     3361 gaagcgattt tagctttgca tcatttgtat cagtttgtta attttatcga ttattgttac
     3421 agcaatgagt ttgtggagcg ttattttgat gagaagtgct taccactttc agcaaacatc
     3481 aaataccgag aaactccaca atctatgata aagttacaag acagtttacc agaactgcct
     3541 gattttcatg aacagatggc tgctcagtcc gtagaagttc aagagactta tactgaaaaa
     3601 cgtgagactg cagcgcaacg gcaagatgtg cctttccata ttgatcaatt atctgaggca
     3661 gagacaagaa agctctttat tgatatcgat ctccgtttag caggatggat atttgaagaa
     3721 aactgtcgtg ttgagatagc cgttgatggt ctcaagcacg gttcaggaat tggttactgt
     3781 gactatgtac tttatggtaa aaatgggaaa attttagcga ttgtggaggc taaaaaagcc
     3841 tctgtcaatc cagaagtagg ggaagtacag gtcaaagaat atgctgaagc tttggagaaa
     3901 catatcggct atcagccaat ttgctttatt acaaatgggt tgaagcacta tatacttgat
     3961 ggtccgaacc gccgccagat tgcaggcttt tactctcaag aagaattgca attagtgatg
     4021 gatagacgtc atcttcaaaa accgcttgag gatatttcta gtaaaattag ggacgatatt
     4081 tccgggcgtc actaccaaaa acatgccatt gcaagcgttt gtgaagcttt ctctaatcat
     4141 cgtagacagg cacttttggt tatggcaact ggggcgggga aaactcgtac agcagtttct
     4201 ctagttgata tcttatcacg tcataactgg gtaaaaaacg ttctcttctt agccgataga
     4261 acttccttgg ttaagcaagc ctatgattcg tttagaaaat tactcccaga tctttccgtt
     4321 tgtaacttct tagaagataa agaaggagct caatcaagtc gtatggtctt ttcaacttat
     4381 ccgaccatga ttggagcgat tagtggtcaa gaagaagtaa atcaacgccc ttttactgtt
     4441 gggcattttg accttatcat aattgacgaa tctcaccgtt ctatttatca gaaatacaag
     4501 tccatttttg attattttga tgcaaggatt gtaggcttaa cagctactcc gcgtcaagat
     4561 ttagataaaa acacctatgg attctttaat ttggagaatg gggttccaac atatgcatat
     4621 gatttggaag aggctgttaa agacggatat ttagtagcct atcattctat cgaaaccaaa
     4681 ctgaaactac ctacggatgg tctacattat gatgatttgt ccgaagaaga aaaggaacat
     4741 tttgatagca aatttgaaga cgatagctgt gaaaaagata ttgatgggag tgtatttaat
     4801 tcctttgttt tcaataaaag tacagtagaa attgttttaa atgaactcat gacaagagga
     4861 attcagacag cctcgggtga tgaaattggt aaaactatta tttttgctaa aaatcatgat
     4921 catgcagaat atatcagagg tatttttaac aaccgctatc ctgaaaaagg gagcgactat
     4981 gctcaggtga ttgattatag tattaagcat tatcagacct tgattgatga ttttaaaatt
     5041 gaggagaagt atcctcaaat tgcgatttct gtcgatatgt tagatacagg tattgatgta
     5101 ccagaggttg ttaatttagt cttcttcaag aaagtacgct ctaaaactaa gttttggcag
     5161 atgattggtc gaggaacccg tctatgtaaa gatttatttg gacctgagca ggataaggaa
     5221 aacttcttgg tatttgatta tggggataat tttgattatt ttcgtgcaga tccaagagat
     5281 ggagagggtc gtcacattgt ttcgttgact cagcgtttat ttaatatcaa agtggacttg
     5341 attcgagaac ttcagggact ccaataccaa gaagatcagt ttgcgagagc ataccgtcag
     5401 cagcttgtct cggaacttca aggtcgtata gagagcttaa atgagttgga cttcagggtt
     5461 cgtatggttt tagatacagt ttatagctat aggaaattgg aaagttggca gaatctaact
     5521 gctgttacaa gtgaaaccat tcaaaaaaat ctctctccgc ttttatttga tgaagataaa
     5581 gaagatgaga tggccaggag atttgatttg tggttgcttc atattcagtt ggggcaactg
     5641 acagctaaat cttccactgt tcatatttcc caagtgatga agacggctag agctctttct
     5701 gctattggca atatcccgca ggtttttgag caggctgaaa ttatcaggaa agtacaggag
     5761 cctgaatttt ggaaagaagt taacttgtct gatttggaaa aaattcgtct tgctattcga
     5821 gatttattac agtttttgga taaaacagac cgtaaaccct actatgttaa ctttgaagat
     5881 cgtatactct ccactgttca cgagaccaca gcatttttgc aggtcaacga tcttcggtct
     5941 tacaatgaaa aagttgagca ttatttgaaa actcatctgg atgaggagtc catttctaag
     6001 ctataccata ataaaaagtt gacatctgat gatatgcttg cacttgaaaa attgctttgg
     6061 gaaaaattag gtagtaaagc agactaccaa agtcattatg aaaataaggc aattccgaga
     6121 ttggttcgtg agattattgg cttagataga gagtctgcca atcgtatttt ttctaaattt
     6181 ttgtcggatg agaatcttaa tgccaggcag atttcatttg taaaattgat tgtagactac
     6241 attgtagaaa atggtttttt agagacgaaa gtgttaacgc aagagccgtt taaatcttat
     6301 ggttctgttc aactactctt ccaacaccaa ctaccagtac ttcgtaatat tgttcaaatc
     6361 attgaactta tcaataatcg agctggagaa gcggcttaaa ttctaaagtg attgccatgc
     6421 tgagactcat ttaaaattaa aaagagtaga aatttatgct atatatgaga agttttatta
     6481 ggaagaatgt catcgttttc ctagaataca gtatcagttg ttaagtggtt gataaatttc
     6541 aaagtagata cttgtaccac gatgtttgtt gatcgagtta ttaacaaaag agctactttg
     6601 attttaaaga aatagaaaac aaaaagccga gcaagaattc aattgcagga gaaaatgaaa
     6661 taatactcaa tgaaaatcaa agagcaaact aggaaactag ctgcaggctg ctcaaaacac
     6721 tgttttgagg ttgcagatgg aagctgacgc ggattgaaga gattttcgaa gagtataaat
     6781 cttcctagga taaagcaaaa cgcatagtat caagggtttt caacacttga tactatgcgt
     6841 tttctgatgt taaagacttt ctaccaggtt tttttaagca taattgttag ttgtagtcat
     6901 ttattattct tcaaagaaaa atggtggggc gaattttttc agttcttcaa agcacttttg
     6961 agcagtatct gcatcttcac agatgataag acagacatca ttaccacaaa gggtagcgat
     7021 agcgtcaggg aagctcaaag tatcaatgat agaaccaaag gattgagcca gtccaggaag
     7081 ggtttttagt aggacttggt gttgaactgg gcgcatccag acaagggcgt cttccatgta
     7141 gagttcgaga cgtttttccc attttgagat ggaaccattg ttaagaacat aataagcgct
     7201 atcttcttcg cggacttttg ataggttcat atttttgatg tcgcgtgaga gggttgcctg
     7261 ggttacttga atgtcgttct tagcaagaag ggcttgcaac tcagcctgtg tatgaatctt
     7321 gttttttgtg ataagagcgc gtataagttg gtggcggtgt tctgatttat tcataataat
     7381 gtaactcctt ttagcaaggt aaggtaagca tggactgagc gaggtcgaca gtcaagtggt
     7441 agtctgtatt gtcacggatg gtgatttcaa agtcagtagt atagaggact aaacggagag
     7501 tgtctccttc ttttagcttg taaatagttg gctgcagttc aaattgaacg tccatccatt
     7561 catctgcagt aatatcctct actaacagta aatcatttct attttgtaaa ttaaggtaac
     7621 cttttgtcac gactcgttgt gcctctggtc taaatggcaa ttcacagaga ttttccaaca
     7681 tgtgatagcg accgttgtca atggttctag cacttaaaat agctggataa ggttgtaggt
     7741 atttcttttg cccaaattct agcagttggg cagataagag ccccttgttt gtactggatt
     7801 tgatacgaag attgagctga gcgcgaccgt ttaggtggag atctttagtc acaggaaggt
     7861 taatagtaat ctgattagct ttcccttgat agagctctgt attgaaggtt tggtatgtct
     7921 taccatagcg ctcaaaatcc ttatctgggt actggttttg aatagcttgc tcttcttgac
     7981 caagtgagaa ggtttcacag ttttcttgcc caccgaagtt atcaagtgat aaccaagtct
     8041 gtggagctgt attgtcctgc cagataacag taggaagttg aaagtctgtt tcctgtccta
     8101 gtaatttctt ggtcaataag gcatttatgg actcacggaa gtcaattgat tgccaattgt
     8161 tcatgtaaac atgggcacca ttatggaaaa agagatgctt gtgtatatga gtaggaagag
     8221 catggaacat ctggtaaaca tgaagtggtt tgacattcca atcctgagaa ccatgagtaa
     8281 agacaacctc tgcctttact ttatgggcat tgagcagata attgcggtca tgccaaaact
     8341 gattgtagtc cccagttttt cggtctagct gagctttcac tttttctaag tcagcttggt
     8401 gagcttcatt gccacggata tagtcgccag ctaagagatt acgagaatag gttaactcag
     8461 caagggagtc aaagtcctca cctggataac cacctgggct agtcaccaga ccgttttcac
     8521 ggtagtagtt gtaccatgat gaaattcctg cctcggcaat gataacttct aaaccatcga
     8581 ctcctgtagt cgcaagacca ttggacatgg tacctagata ggaaagtcct gttgtagcaa
     8641 cttttccgtt tgaccaatca gccttgactt gacgctggcg cgtgtgatca gtaaaggcac
     8701 ggcaacgacc gttaagccaa tcgatgacgt ttttataagc ctcgatttgc tggtagtctc
     8761 cattagtcat gaaacctgtc gagtctttgg taccaacacc tgagacatag agattggcaa
     8821 agcctctcgg aaggaagtag tcgtttagtg tatagctaga gttgatgtga gttagctttt
     8881 cctcagcctc tgctataagc tcagctttac cttggggttg gacgagattt agttgaggtt
     8941 tctctagctc aatcttgtga ggaagcttaa cctcaagctc gccctccatc ttgtagagag
     9001 ccttgtcact agccttgtca ttggttccct gatgataagg gctggctgtc atgatggcag
     9061 ggatttttcc atcaaaacga gggcgaataa tgctaacctt tactaggtct gatagccctt
     9121 tttggtcagt atcgacacga gactcaacgt aaacgacttc acgaatgaca tcctggttag
     9181 aaaaagtagc caaactcttg ccgttaaagt agtggtagtc attatcctcc ggaataagac
     9241 catcactaac aagttggtcg ataagagtat ttcctttttt ggtgcgagta ttgagtaact
     9301 gatagagatt ttcaatcaag tcaccatata taatgggaaa tccagtttct ttacgaaaaa
     9361 cgtcactatc ttcgaagtca accaaataag aaaagcctaa aagttgaaaa gcaacagtat
     9421 aaaaaatatc tgctgtcagt tcatcttctg attgaaaaaa tgtcagcagg tctgtttttt
     9481 tatcagctgc taggatagaa agtgggtagt tggtgtcttg ataagtgaaa aagaaacgac
     9541 gtaaaaaggt ttcaagtgag tctttgtgat tggctgtatt ttgtaaatca aagccacatt
     9601 tttttagttc agataagaca ttttcttttg gaaaattgat ataactatat tgattaaaac
     9661 gcatagaacc tccatataga atgacagtta aggttattat atcaaaa
//
LOCUS       DAVHIT010000074         9649 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVHIT010000074 DAVHIT010000000
VERSION     DAVHIT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.075"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..212)
                     /locus_tag="VVN16_001635"
     CDS             complement(<1..212)
                     /locus_tag="VVN16_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5572976.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNIRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEMSNKVL"
     gene            complement(308..1288)
                     /gene="dusB"
                     /locus_tag="VVN16_001636"
     CDS             complement(308..1288)
                     /gene="dusB"
                     /locus_tag="VVN16_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU5572977.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1275..2147)
                     /gene="hslO"
                     /locus_tag="VVN16_001637"
     CDS             complement(1275..2147)
                     /gene="hslO"
                     /locus_tag="VVN16_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU5572978.1"
                     /translation="MDKTIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKIAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2312..3739
                     /locus_tag="VVN16_001638"
     CDS             2312..3739
                     /locus_tag="VVN16_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5572979.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLMQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3897..5405
                     /gene="glpK"
                     /locus_tag="VVN16_001639"
     CDS             3897..5405
                     /gene="glpK"
                     /locus_tag="VVN16_001639"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU5572980.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5447..7273
                     /gene="glpO"
                     /locus_tag="VVN16_001640"
     CDS             5447..7273
                     /gene="glpO"
                     /locus_tag="VVN16_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU5572981.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDGNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEVFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7343..8047
                     /locus_tag="VVN16_001641"
     CDS             7343..8047
                     /locus_tag="VVN16_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU5572982.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     VPVIGAALAVLVFSLF"
     gene            8344..8547
                     /locus_tag="VVN16_001642"
     CDS             8344..8547
                     /locus_tag="VVN16_001642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572983.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(8634..9221)
                     /locus_tag="VVN16_001643"
     CDS             complement(8634..9221)
                     /locus_tag="VVN16_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5572984.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2768 a         1909 c         2173 g         2799 t
ORIGIN      
        1 aggactttat tactcatttc ttgttttaaa tcaggagaat agtaacgatt ttttcctttt
       61 ttgacgaact ctattccgta acgatcaatc aatttaatca tgtacctaat attagaattg
      121 tttatcccaa atttatttga aagcttctct aagctatatc cttgttttct aagttcatag
      181 atctgaactt tatcatcata agttaatttc ataataaaaa caccccaaaa gttagatttt
      241 ttctgtctaa cttttggggt gcagttcatt ttgagctttg ccgtttttct gagttgtaga
      301 tgtaagacta tgccttctcc aattgcaaga gggcttcaat ctctgccagg gtgctagctt
      361 gcgaaatggc tccacggagt ttggcagcgc cagatgttcc acggaggtag tgaggagcga
      421 ggccgcggaa ttcacgaact gcgacgtttt ctcctttgag gttaatcaat cgtttcaagt
      481 gttcgtaggc gatcttcatc ttgtcttcaa aggtcaaatc aggtaggatt tctcctgttt
      541 caaagtaatg gttgatttgg ttgaagaggt aaggatttcc catggcagct cggccaatca
      601 tgactgcgtc agcaccaact tcttcgatgc gttgcttggc ttcttggaca gtacggatat
      661 caccgttggc gatgaatgga atcttggtta gagcttgggc aaccttgtaa agggtctcaa
      721 ggtctgcgtg gccagtatac atttgttcac gggtacggcc atgcatggcg agggcagaaa
      781 cacctgcagc ctcagcagcg agggcatttt ctactgccag agatgggtcc gcccagccgg
      841 tacgcatttt gacagtaagt gggatatcaa ggacagactg gaccttgttg atgatagagt
      901 agatcttgtc aggatccttg agccacatag caccagcttc gttcttcacg attttgttga
      961 cagggcagcc catgttgata tcgacgatat cggtcttagt gttttcttgg ataaattctg
     1021 ctgcgcgtgc taggctgtct tcatcgctac caaaaagttg gatagagaca gggttttcgc
     1081 cctcatcgat atgaagcatg tgcagggttt tttcgttgtt gtattggatt cccttgtcag
     1141 agaccatttc cattacaacg agtccagctc cgagctcttt tgcgatggta cgaaaggctg
     1201 agttggtcac gccagccata ggcgctaaaa cggtacgatt gggaatctca acattgccaa
     1261 tcataaaagg tgtattaaga tttgtcacga atgagttcct ccaggtcctt ttcatcaaag
     1321 ttgtaagtag tttggcagaa ttgacaagtg atttctaccc cgtggtcttc ctctttcatt
     1381 tcctgtaagt ctgagcttgg aaggctggca agagcgttca taaagcgttc atggctacag
     1441 tcacattgga aacggatttc ttcttcagaa agacgcttgt aggcttcgtc cccgtagata
     1501 gccttgagga gggcttcgat atggtcgtcg ctttcgagaa gagtagagat agctggcatt
     1561 tcttggatgc gtttttcaaa gcgagcaatc tcttctttct tggctcctgg caagacttga
     1621 actaggaaac cacctgcaat cttgaccttg tcttcctcgt ccaaaaggac attgaggccg
     1681 accgctgaag gcgtttgttg gctttcagta aggtaaaagg caaggtcttc accgatttct
     1741 ccagagatga ggggagttat agagttgtaa ggatttccag taccgtagtc tgtgataacg
     1801 aggaattgac catttccaac aaaaggtccg actaggactt caccagtcgc agtctttttg
     1861 atgtcaacac caggattttg aacgtagcct ttgacgttcc ccttggtatc agcgacggtg
     1921 ataatagcac ctagagagct agatcccaac accttaactg taagtttggt atttcctttt
     1981 tcattggctg cgagaatctg gctggcgata agagttcgac caagcgctac agttgagcta
     2041 gcttgggttt gatgtttttc ttgagcagtg cggacggttt cagtgctatc aaggacaaaa
     2101 gcacgaaagg ctccgctttc tgatatagtt ttaatagttt tatccatagc tactatttta
     2161 gcataaaaat gcccaaaggg ggagccgtgt gtttactgat tttcaggata atggaccagg
     2221 aaatcagcat gaaaataaaa agagaaacag attattttag catttgtaag atttatgcta
     2281 tgcttaaggt agaaaatgaa aggggtaaca aatgtattta ggagatttga tggagaaagc
     2341 cgagtgtggt caattttcaa tcctttcctt tctattacaa gagtctcaga cgaccgtcaa
     2401 ggctgtaatg gaagaaacag gattttcaaa agcaacccta accaaatatg tcaccctgct
     2461 caatgacaag gctttggata gtggcttaga gctggctatt cactcagaag atgaaaatct
     2521 gcgtctgtct atcggtgcag ctaccaaggg gagagatatt cggagcttgt ttttggagag
     2581 tgctgttaaa taccagattt tggtttatct tctctaccac caacagtttt tagcccatca
     2641 gctggctcaa gaattggtga ttagcgaggc tacgcttggt cgtcacttgg ctggtttaaa
     2701 tcagattttg tcagaatttg atttatccat ccaaaatgga cgttggcgag gtccagagca
     2761 tcagattcgc tatttctatt tctgtctttt ccgaaaggtc tggtcgagtc aggaatggga
     2821 aggtcacatg cagaaaccag agagaaaaca ggagattgcc aatttagagg aaatctgcgg
     2881 tgcaagtttg tctgtggggc agaaattgga cttggttctc tgggctcaca tcagtcaaca
     2941 acgtcttcgg gtcaatgctt gtcagtttca agtcatagaa gagaaaatgc gagggtattt
     3001 tgacaatatt ttctaccttc gtttgctgag aaaggttccg tccttttttg ctgggcaaca
     3061 tattccacta ggagttgagg atggtgagat gatgatattc ttctcttttc tcctatctca
     3121 tcgcattctt cctcttcata ctatggagta tattcttggt tttggagggc agttggcaga
     3181 tttactgatg caattgattc aagaaatgaa gaaggaggaa ctattggggg attatacaga
     3241 ggaccatgtc acctatgaac tcagtcagct ttgtgctcaa gtctatctct ataagggcta
     3301 tattttacag gatcgctaca agtaccagtt agagaatcgt catccatatt tactgatgga
     3361 acatgatttt aaagagacag cagaggagat ttttcatgct ctacctgctt ttcaacaggg
     3421 gacagattta gataagaaga ttctctggga atggctccag ttaatcgaat atatggctga
     3481 aaacggtggc cagcatatgc ggattggtct ggatttgaca tctggttttc ttgtcttttc
     3541 aaggatggca gccattttga aacggtattt ggaatacaat cgttttatta ccattgaagc
     3601 ttatgaccct agtcggcatt atgatttgct ggttaccaat aacccgattc ataagaagga
     3661 acagacccca gtctattatt taaaaaatga cttggatatg gaagatttgg cagggattcg
     3721 tcagttatta ttcacttaaa aggcttggtt aatccaggtc ttttttgtga aattcacaca
     3781 atctcctcac attttttaaa aattaaaaaa agttgataaa caagaaagcg ctttattttg
     3841 tatactagta agtgtaaaga ggaaacacct caagatcttt atcaggagga cagtacatgt
     3901 cacaagaaaa atacatcatg gccattgacc agggaactac aagttctcgt gccatcattt
     3961 tcaacaaaaa aggggaaaag gttagctcga gtcaaaaaga gtttacccag attttccctc
     4021 aagcaggttg ggttgagcac aatgccaatg aaatttggaa ctctgttcag tcagttattg
     4081 cgggtgcttt catcgaaagt ggtgtcaagc caaatcaaat cgaggcaatc gggattacca
     4141 accaacgtga aacaacggtt gtctgggata agaaaacagg acttcctatc tacaatgcta
     4201 tcgtttggca gtcacgccag acagcacctt tggctgagca actaaaaagc caaggttatg
     4261 tggaaaaatt ccatgaaaag actggtttga ttattgatgc ttacttctct gctaccaagg
     4321 ttcgttggat tttggatcat gtagaaggtg ctcaagagcg agcagaaaaa ggggaattgc
     4381 tctttggtac tatcgatact tggttggttt ggaaattgac tgacggtgcg gctcacgtga
     4441 ctgactactc aaatgcagct cgtaccatgc tttataacat taaagaactc aaatgggatg
     4501 atgagatttt ggaaatcctt aacattccga aggctatact tccagaagtt cgttctaact
     4561 ccgaaatcta cggcaagaca gctccattcc atttctacgg tggagaggtg ccaatctcag
     4621 gtatggctgg ggaccaacaa gcagccctct ttggacagtt ggcttttgag ccaggtatgg
     4681 ttaagaatac ttatggaaca ggctctttca tcatcatgaa tactggggaa gagatgcagt
     4741 tgtctgaaaa caacctcttg acaaccattg gttacggaat caacggtaag gtttattatg
     4801 ccttggaagg ttctatcttc atcgcaggaa gtgctattca gtggcttcgt gacggtcttc
     4861 gcatggttga aaattcacca gaatctgaaa aatacgctcg tgattctcac aacaacgatg
     4921 aagtttatgt cgttccagcc tttacaggtc taggcgctcc atactggaac caaaatgctc
     4981 gtggttccgt ctttggtttg actcgtggaa caagcaaaga agactttatc aaggcgactt
     5041 tgcaatctat tgcttatcaa gtgcgtgata tcatcgacac catgcaagtg gatactcaga
     5101 ccgccattca agtactgaag gtggatggtg gtgcagccat gaacaacttc ctcatgcagt
     5161 tccaggcgga tattttaggc attgacattg cacgtgctaa aaacctggaa acaacagctc
     5221 taggagcggc cttcctagca ggtttgtcag tagggtactg gaaagacttg gacgagttga
     5281 aactcttgaa cgagacagga gaactctttg agccatctat gaacgaatct cgcaaggaac
     5341 aactctacaa gggctggaag aaggctgtga aagcaactca agtctttgcg gaagtagacg
     5401 actaatactg gcagaataaa gcgatttatt tagaaagtgt gtaaatatgg aattttcaaa
     5461 gaaaacacgt gaattgtcaa ttaaaaaaat gcaggaacgt accctggatc tcttgattat
     5521 cggtggagga atcacaggag ctggtgtagc cttgcaggcg gcagctagcg gtcttgagac
     5581 tggtttgatt gaaatgcaag actttgcaga aggaacatct agtcgttcaa caaaattggt
     5641 tcacggagga cttcgttacc tcaaacaatt tgacgtagaa gtggtctcag atacggtttc
     5701 tgaacgtgca gtggttcaac aaatcgctcc acacattcca aaaccagacc caatgctctt
     5761 accagtttac gatgaagatg gagcaacctt tagcctcttc cgtcttaaag tagccatgga
     5821 cttgtacgac ctcttggcag gtgttagcaa cacaccaact gcgaacaagg ttttgagcaa
     5881 ggatcaagtc ttggaacgcc agccaaactt gaagaaggaa ggcttggtag gaggtggagt
     5941 gtatcttgac ttccgtaaca acgatgcgcg tctcgtgatt gaaaacatca aacgtgccaa
     6001 ccaagacggt gccctcattg ccaaccacgt gaaggcagaa ggcttcctct ttgacgaaag
     6061 tggcaagatt acaggtgttg tagctcgtga tctcttgaca gaccaagtat ttgaaatcaa
     6121 ggcccgtctg gttattaata caacaggtcc ttggagtgat aaagtacgta atttgtctaa
     6181 taagggaacg caattctcac aaatgcgccc aactaaggga gttcacttgg tagtggattc
     6241 aagcaaaatc aaggtttcac agccagttta ctttgacaca ggtttgggtg acggtcgtat
     6301 ggtctttgtt ctcccacgtg aaaacaagac ttactttggt acaactgata cagactacac
     6361 aggtgatttg gagcatccaa aagtaactca agaagatgta gattatctac ttggcattgt
     6421 caacaaccgc tttccagaat ccaacatcac cattgatgat atcgaaagca gctgggcagg
     6481 tcttcgtcca ttgattgcag ggaacagtgc ctctgactat aatggtggaa ataacggtac
     6541 catcagtgat gaaagctttg acaacttgat tgcgactgtt gaatcttatc tctccaaaga
     6601 aaaaacacgt gaagatgttg agtctgctgt cagcaagctt gaaagtagca catctgagaa
     6661 acatttggat ccatctgcag tttctcgtgg gtctagcttg gaccgtgatg gcaatggtct
     6721 tttgactctt gctggtggta aaatcacaga ctaccgtaag atggctgaag gagctatgga
     6781 gcgcgtggtt gacatcctca aagcagaatt tgaccgtagc tttaaattga tcaattctaa
     6841 aacttaccct gtttcaggtg gagaattgaa cccagcaaat gtggattcag aaatcgaagt
     6901 ctttgcgcaa cttggagttt cacgtggttt ggatagcaag gaagctcact atctggcaaa
     6961 tctttacggt tcaaatgcac cgaaagtctt tgcacttgct cacagcttgg aacaagcgcc
     7021 aggactcagc ttggcagata ctttgtccct tcactatgca atgcgcaacg agttggctct
     7081 tagcccagtt gacttccttc ttcgtcgtac caaccatatg ctctttatgc gtgatagctt
     7141 ggatagcatc gttgagccag ttttggatga aatgggacga ttctatgact ggacagaaga
     7201 agaaaaagca acttaccgtg ctgatgtcga agcagctctc gctaacaacg atttagcaga
     7261 attaaaaaat taagaaaaaa taaaagaggt ggagggcagc attccttgtc gcccgtccct
     7321 tctttttaat ggagacagaa agatgatgaa tgaattattt ggagaatttc tagggacttt
     7381 aatcctgatt cttctaggaa atggtgttgt tgcaggtgtg gttcttccaa aaaccaagag
     7441 caacagtgca ggttggattg tgattactat gggttggggg attgcagttg cggttgcagt
     7501 ctttgtatct ggcaagctca gtccagctca tttaaatcca gctgtgacag ttggtgtagc
     7561 tttaaaaggt gacttgcctt gggcttccgt tttgccttat atcttagccc agttcgcagg
     7621 ggctatgctg ggtcagattt tggtttggtt gcaattcaaa ccgcattatg aggtagaaga
     7681 aaatgcaggc aatatcctgg caaccttcag tactggacca gccatcaagg atactgtatc
     7741 aaacttgatt agcgaaatcc ttggaacctt tgttttggtg ttgacaatct ttgctttggg
     7801 tctttacgat tttcaggcag gtatcggaac ctttgcagtg ggaactttga ttgtcggtat
     7861 cggtctatca ctaggtggga caacaggtta tgccttgaac ccagctcgtg accttggacc
     7921 tcgtatcatg cacagcatct tgccaattcc aaacaaggga gacggagact ggtcttacgc
     7981 ttggattcct gttgtagtcc ctgttatcgg agcagccttg gcagtgcttg tattctcact
     8041 tttctagttt atactcttcg aaaatcaaat tcaaaccacg tcagcgtcgc cttaccgtac
     8101 tcaagtacag cttgcggcta gcttcctagt ttgctctttg attttcattg agtattagaa
     8161 aacaattatg ttgatagagc ttgggcaaga gcccaatttc agcaaaaaat gaagtaaatc
     8221 ttctcataat aaaacgcatc atatcaagca cgaaaattcc acgaggtcaa ctacagtcag
     8281 aaagctgaac aacaagccaa aacgcccaaa aaaggcggca aaaagcaagc acctgcaagc
     8341 aacgtgccga aatggtcaaa tcctgattat gtcaacgaat tagacccaaa aatcgttgat
     8401 atgctagtag aatttcacaa gtcacaaggc actttggaaa ctcccgaggc gcaagcagaa
     8461 atcgcccaaa aacgtgaaga aatcgagcaa aggagagctg agcttgaggg taaaaaacaa
     8521 gagcttttga accgcttgaa caaatagagt ttcgcaagta ttatgcttac aaattacttg
     8581 agcaattaac taaaatataa accctgcctt tatatctagg cagggtttat attttagaaa
     8641 ttcacgtagg ttgttacggt ttttacatac ccagtatagt ttgagtttct atagtattca
     8701 gtgataaact tccattttct ttgagcaaca tggatataag tacttgttat gtagtatgga
     8761 tatgggcttt gtgaatccaa gtaagactga taagcttgta taccaaaata tgctccacca
     8821 attattgcac cccatggacc ccccaataaa gcacctatcc taccaatcat ataactgatt
     8881 ccagcaccag tcatgaagtt agcgaatgtg ttagcttgtt tattcccatg tattgtgttg
     8941 acgtaattcc aaacattagg atcgtatgat ctaaaagata tatttaggtc gatttcattc
     9001 ttttgataag ccatataaaa tgccccattg atatagacgc cgtcagcacg tcgttcaata
     9061 gtgtctacac ttccatctgg attgacaacc tcaagaactt catcgcttaa aatatttact
     9121 tgcgtatctc cgaaccgcac tgatgagcca ttctcaaact gagcctcacc agatacaact
     9181 ttagagtttg ccgataagct atcatcagca aaaacaaaca agcgacgggg aaatgctaga
     9241 catacagaaa acagacataa ctagcaaaca catgcattta aacatcttag acataacgga
     9301 aactcctttg tatttttgat ttttttcaac ttttattata caataaaacc aaataaaaag
     9361 aaagcggtaa caatatgctt aatgcgaaaa ttttttatat atttttatgt ttgatcgtta
     9421 tcgaaactac aggcttgttg ttgttgaaaa gaggtctcga aatgggttat ttagacacag
     9481 aagctattat cctcgcagtt ttttcatttg ctttttacaa cctatgttca ttcgcttggg
     9541 tctgctctac aataaaaaac aataaaaaaa taaatagacg tattttcaaa aaaaaaaaaa
     9601 atgcatattt atcttagcaa aacgacgatt taaatcgtcg tttttttgt
//
LOCUS       DAVHIT010000075         9645 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVHIT010000075 DAVHIT010000000
VERSION     DAVHIT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.076"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..979
                     /locus_tag="VVN16_001644"
                     /pseudo
     CDS             1..979
                     /locus_tag="VVN16_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1122..2036)
                     /locus_tag="VVN16_001645"
     CDS             complement(1122..2036)
                     /locus_tag="VVN16_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU5572985.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LQDSPGTNNFSLPNVSPTYGQYYTNQSEVIIGKNDLVKVKNTLQYPYSTSAYVESEFR
                     GVKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTPARTPNSVPFGRIK
                     VKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLTGITVTITGY
                     PSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASNRVVGVHTLGDGAN
                     QINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(2186..2281)
                     /locus_tag="VVN16_001646"
                     /pseudo
     CDS             complement(2186..2281)
                     /locus_tag="VVN16_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2358..2669)
                     /locus_tag="VVN16_001647"
     CDS             complement(2358..2669)
                     /locus_tag="VVN16_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU5572986.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2594..2836)
                     /locus_tag="VVN16_001648"
     CDS             complement(2594..2836)
                     /locus_tag="VVN16_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU5572987.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(2837..3469)
                     /locus_tag="VVN16_001649"
     CDS             complement(2837..3469)
                     /locus_tag="VVN16_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5572988.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(3483..4478)
                     /locus_tag="VVN16_001650"
     CDS             complement(3483..4478)
                     /locus_tag="VVN16_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5572989.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDQDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4475..5173)
                     /locus_tag="VVN16_001651"
     CDS             complement(4475..5173)
                     /locus_tag="VVN16_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU5572990.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5251..6261)
                     /locus_tag="VVN16_001652"
     CDS             complement(5251..6261)
                     /locus_tag="VVN16_001652"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU5572991.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6245..7252)
                     /locus_tag="VVN16_001653"
     CDS             complement(6245..7252)
                     /locus_tag="VVN16_001653"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU5572992.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAESTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7239..8192)
                     /gene="mvaD"
                     /locus_tag="VVN16_001654"
     CDS             complement(7239..8192)
                     /gene="mvaD"
                     /locus_tag="VVN16_001654"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU5572993.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(8174..9052)
                     /gene="mvk"
                     /locus_tag="VVN16_001655"
     CDS             complement(8174..9052)
                     /gene="mvk"
                     /locus_tag="VVN16_001655"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU5572994.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9227..9505)
                     /locus_tag="VVN16_001656"
     CDS             complement(9227..9505)
                     /locus_tag="VVN16_001656"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5572995.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
BASE COUNT         2898 a         2197 c         1742 g         2808 t
ORIGIN      
        1 gaaaaaatcg ttactattct cctgatttaa aacaagaaat gattaataaa gtctgacatg
       61 aaggctggac taaagataga gtttctcttg aatactgcct cccaagtcgt acgatacttc
      121 ttaactggct agcacaatac aggaaaaacg ggtatactat tgttgagaaa acaagaggga
      181 gagtacctga gagcggagaa tgccatccta aaaaagttaa gagaactccg attgaaggag
      241 gaaaaagaga aagaagaaag atagaaattg ttcaagaatt aatgactgag ttttcgttag
      301 atcttcttct aaaagccatt aaactagctc gttggaccta ctactatcac ttgaaacagc
      361 tagacaaacc agataaggac caagagctta aagctgaaat tcaatccatt tttatcgaac
      421 acaagggaaa ttatacttat cgccgggttc atttagaact aagaaatcgt ggttatctgg
      481 taaatcataa aagagttcaa ggcttgatga aagtactcaa tttacaagct aaaatgcgac
      541 agaaacgaaa ataaaggaga cgttggtaag aaggcagaga atctcattca aggccaattt
      601 gaaggctcta aaacaatgga aaagtgctac acagatgtga cagaatttgc cattccagca
      661 agtactcaaa agctttactt atcaccagtt ttagatggct ttaacagtga aattatcgcc
      721 tataatcttt caacttcacc caacttagaa caagtacaaa caatgttgga acaggcattc
      781 acagagaagc actatggtgg agtccttctt tgggattctg aaatcggaaa tgttttacgg
      841 ttatgagaag tcgtttcagt cgtttaagca attggaacaa gctattgtag actatattga
      901 ttactacaac aataagagaa ttaaggtaaa actaaaagga cttagtcctg tgcagtacag
      961 aactaaatcc tttggataaa ttatttgtct aacttttggg gggcagtaca gaaaaacctt
     1021 gatactatgc gttttttcct attttaaaga cttttatcca cctattatac aaagttaagt
     1081 atccatcaca gtttacaatt agccttcatt acctaaccca ttcaccatcc ttcaagagag
     1141 taacctttaa gaaccgaata aataaatggc aaatttcgtt catttaattt aactgcactg
     1201 ttaatttgat tagctccatc tcctaaagta tgcactccta ctacgcggtt actagcatca
     1261 taaactgtag atccactaga cccctctaaa gtatcaactt ggtagtccaa gaacatgcca
     1321 tcatcactta aaacttgttt tttatctgta tacatttgat gaattttaaa attatatgat
     1381 ggatagcctg tgatagtcac agttattcct gtcaaatttt tttgactagt aggaagaccc
     1441 aaagtcccta attttgcacc aatgggctct tctagaatta ataaagccaa gtcatattcc
     1501 cttgcatcct tagaatttaa atttctaaat tccttcaaat aacgaacttc ttttactttt
     1561 atccgaccaa acggcacact atttggagta cgagcaggag taacatatag ttcttctgca
     1621 tctttaccaa actcatgttt ataaatatta tgagcagcag taataaggac attatccttt
     1681 ataaaattgg cactccctcg ataagttaca tctttaccat ttttcacacc acgaaattct
     1741 gactctacat aagctgatgt agaataagga tattgtaaag tattttttac cttaactaaa
     1801 tcatttttac caataatgac ttctgactga tttgtataat attgaccata agtcggtgaa
     1861 acattgggaa gtgaaaaatt attagttcct ggactatctt gtaatgtctc acttacctca
     1921 tattcttcac ctgtactaga aataacactc aaagcagata ctgtcgataa ctggctagcc
     1981 aataaagtac tcgcaataat tgaaataccc aattttttat aaacagtttt cttcattatt
     2041 gtatcctcct aatgtaatta tagcgtacta ttctaaattt cttaatctac tatagaatca
     2101 agaaatctac caccttcttt aaataccctc cattatcaca taaacaggta aacttttcaa
     2161 ttaatgactg cgcttttcaa tcacgctaga ggtacttact tgcttctttg atactaagtt
     2221 cagccattct ttccttgttt ttctcaataa agcatgttac ccaagtggga ttcgttctgg
     2281 tggtcaatag cgacacgtct cagccagata ttatctgata ggctccattt tatactcaat
     2341 gaaaatcaaa gagcaaacta ggaagctagc cgcagattgc tcaaaacact gttttgaggt
     2401 tgcagataga gctgacgtgg tttgaagaga ttttcgaaga gtattaagat tatttcttct
     2461 agttcagggt gttcatacac caaactccct actactcgat ctaggatatc taccgtgtcc
     2521 caccaggatt ttgtcacgac taactgctct agcttaggca aatcggtttc ctttagataa
     2581 gactgcattg ctttcaaata gttagcagcc acatattggt attttctagg ctccttttcc
     2641 cagcaagtgt ctgcaaaatc ccaatcgata atctttgttt ttttcgcttc tggtaaatat
     2701 ttttatagag tttatttctt tcaggcaccg caatacctag aaaagaaaat tgatggcgca
     2761 tataggcttc catggacctt gcttttttag agtcttttgc tgcttctagc tcctcaagta
     2821 aatctgctaa actcatctaa aactcctcat gcccgaccaa atggtgctga aaggcataga
     2881 cagccgcctg ggtacgatcg ctaacttcaa gtttggcaag aatgttggac acgtgggtct
     2941 tgaccgtctt gagagagata aagaggtcat ctgcgatgcg ctgattttcg tagcccttgg
     3001 cgatgagttg gagaacatct cgctcacgcg cagtcaattc ttcatgaagt tccatatgat
     3061 tgcggtggta ttcaaccttc ttgctaacct cttgctcaat ggccagctcg ccagcagcta
     3121 ccttactgac ggcatgaagc aattcatctg cactagaagt cttgagcata tagcctttgg
     3181 caccagcatc taagactggc atgatttttt cattatccaa ataagaggtc acaatcaaaa
     3241 tcttggcttc aggccattct ttaaggattg ctaaggtcgc gtcaatccca ttcatctcag
     3301 gcatgacaat atccatgaca atgacatctg gacgcagttc caaggccaag tcaatccctt
     3361 gagacccgtt ggacgcctca cccacaactt ctacatcgtc ttggaggtca aagtagcttt
     3421 tcaagcccaa acggaccatt tcatggtcat ctactagtaa aattttcatc tttactcctt
     3481 tatcattcct tatctaacag gggaatacgg atatcaactg ccagcccttg cttgggagct
     3541 gttaataact gaaccgtccc tgccatatct tcaacccgct ccttgatatt tcgcagtcca
     3601 taactcaagt cgtctaagct ccctaactgg aaaccaatcc cattgtccac caccttcagt
     3661 tgcaattcaa catctgtctg atagaggtag acatctaggc aagatgcctg ggcatggcgg
     3721 agcgtattgc taatcaactc ttgcaggata cggaaaatat gctcctcgat tttcttaggc
     3781 aatttcgtca tattctgctt gagactaacc ctaagatcac tcttgtcctc aagctctttt
     3841 aagagaattt gaatcccttc tatcaagctc ttctgctcca gttcaactgg tcgcaaatgc
     3901 aagagcaaaa cccgcaaatc cttctgggct gtttctaaaa tagctgtgac actctgcaac
     3961 tgggtctgca tcttttctct atccaatttc aaagcctgct gactgatacc cgataaaatc
     4021 atgtgggccg caaacaactc ctgactgact gtatcgtgca aatcccgagc aattcgcttc
     4081 cgttccttct cgatgatttc ctcttcctga gcaaggctat gattttcagc tttttgaaga
     4141 gcctctgtca aaaggttaag tttacctgat aaggacttga aactggcatc caaatcttga
     4201 tctacaacct gaactacttc ttgccctgcc aataaacgct tgagattagc ctgcattttt
     4261 cttagagaaa gctcttcgat ccctcgccaa aacagggcta agagacaggt catggacatg
     4321 ctgaaaacca acaataaaaa gacaaatttt tctgtttttt cgacatcgtg caaaaagata
     4381 gaccagtcaa aatcaagtat ttccagcaag ctgtgggaga aaaaaaagac aaataggaag
     4441 gaggtgagag caataatgac ataggcttgt tttttcatcc tctaaccacc tccacatcac
     4501 caatcatagt ggtcaagaaa atcttgacac tcttgttact cttgagatag tcttttgttt
     4561 cttgatgata gtgttcattg cggagggctc gcttgggctg gttgaaaaaa gtcaaatccc
     4621 catagagaca gttaacgctg agactgactt ccacatctac aggtacgatg attttggtcg
     4681 ttcctaccat ctttctgagg ataatgacat tgtcatgatt ggttaagatg accctctcca
     4741 gatgaatagt gtccttaccc atgaagcgaa agagattgat atcatcgaat tggcaagtct
     4801 ggtagcttga aaaatgatga agatttccaa accaacgatt tttctccttc ttaaccgtca
     4861 cgacctcttc aaaaaccaaa ttggtctgct ctttttcctg gttcatcatc gggtaaagaa
     4921 gaaagaggct atagataacc gcaacaaaaa tagctagaat cacaaaagga ttgagcataa
     4981 cgatgaaaaa gaagagaatg gttgccgcta ctaaaagaag attatttccc tctttaccag
     5041 tgtagtagcg aatcaaaagc aaaaagagga atagtatcag cagaaaacgc gaaaaatgct
     5101 ctgataccat caaaatcaga gctcctgtca gaagacaggc ttcgataaat aaaaagattt
     5161 taaattttct cataggttca tcctctccct tctattttat cacaattcaa aaaagtcacc
     5221 tcagtctgag gatggaaaaa aaggcgctgg ttacgccttt ttcatctgat cctttgcttc
     5281 ttttaatttt ccataaagaa gatagtctac tttttgtaga tctgctatgg tggcacagtt
     5341 aagggaacac ataatcaagc gtagatctgc tttccagcct tggacaatgc caatcacttc
     5401 ttcaactgtg taggtttcaa ccaattccag aacggttcgt gacaatccca cagccttagc
     5461 accaaaaacc aagcacttaa tcatatccag cggattccga acccctccac taaccaagag
     5521 ttcgacctta tctttccatt cttgggcatt gagaagggcc tgcatggtag actgacccca
     5581 ttgattgagg taatcacgct ggccactacg acggttttcg atataggcaa agctggtgcc
     5641 accacgaccc gataggtcca ctgtacgaac accgaattca taggctcttt cgattgtctt
     5701 ggcatccatt ccaaagccca cttccttgag gacaatagga acgggaattt gcttgctata
     5761 atctgctaga tgcgattgcc agcttctaaa cttcctttct ccctcgggca tgagtaattc
     5821 ctgcatgaca ttgacatgca cttgcaatag aacaggattc atctcttcta cagtctgaag
     5881 tcctaactcg acaggtttgt ccaatccaat attggttcca aggaggagat tgggatgact
     5941 agacttgaca gaaaaagaat catccgttgg atttttgagg gctgcgctat aagaacccgt
     6001 tacaaataaa ataccacagg attccgccac ctgagccagc ttttgattga tttctcttcc
     6061 cttattactt ccaccagtca tggcattgat ataaaaagga aagtcccact ttcgaccagc
     6121 aaactctgtc gaaagatcga tttcatccag attgtaaaga ggcaaggaag aatgaatcag
     6181 ctccacctca tcaaagctat tataggaact tttctgctca agggcataga ggatatgctc
     6241 gtccttacga tttgtcgtca tgtcctatcc tttcttgata taagagctca atccccagat
     6301 cggcccaacg attttttaag gttttggttg attccgcatc aaaactcagg gcgatgccac
     6361 agtcaccacc accagcacca ctactcttgg caacggcctg caaatcttga ctggcttctt
     6421 tcaactgtct aagcaaaggc gtgtaaatat ctgtactcaa gccttctaaa agcttgctgg
     6481 ctacttctac ttgctcgata actttttcgg ctttcccctg ttccaaggct tcgaccaaag
     6541 aaaccaccgt ttcttttgag gaacttaaaa aattttgatt gatattttgc ttgatttgct
     6601 ggaccatgtg actcgataca gccacttcct tggtccatcc cactaagaaa tcacattcta
     6661 aagttggttt cacttgtgaa attgaaaagc cccaatcacg ctccagaact gtcgccaagt
     6721 tttcttcttc taaccaagca gccaccttct ggcgatcaaa tgactggtag agaaccaaat
     6781 cctctgccac aatacaggca aggtcgccca tggaaccatt gtctcctcgc ttaagcaaga
     6841 cagcgctagt cagcttgaac aagagctcct gatcaacaga aacatcatac agagccagta
     6901 aagccttgac aaccaagaca acgacgctgc cactagaacc tagaccaaac tttttccctt
     6961 ctcgttccat tttgccacag atttctagag aaaaaggtct taaattctga ccacgaacag
     7021 cgaggaagtc tcccatcaaa gcaatcgttt cttgaatcaa gctatagtca ggattaggcc
     7081 ttaagtccac tgcgaaatca aacatatctg aatagatacg gtagctgtca gaaaaagcaa
     7141 tctcagccct catatagatg ggaatatcct ttatcaaagc taactgccct ggctctaaaa
     7201 tagcatattc acctgcccaa tagagttttc cgcaagtttt aacagcaatc atcttgactc
     7261 aaatcctttg tttttgacac aatcaagcga taacgatgac cgaaaatttc tgataaatgc
     7321 tccaagtctt tctcctgaca gaagacctta acattgggac cagcatccat ggtaaagtag
     7381 caggcctctc ctttctcacg aagctggcga acaaagtcca tagcctcata agaggcatcc
     7441 gtcagataag aaaaggctgg actagcagtc tttgtcgtag catgcatagc cagggcattt
     7501 ttctccgtta attctccaat cttggcaaaa tcattttcct tgagataaat cagcatatcc
     7561 tgatagtcct tctcagactg acgaacccag tcgtcgaaag tcgtcgaggt ttccacacaa
     7621 agtttcatcc cgtcacggct agagattggt tttttcttgt cctctagcac caacataatc
     7681 atagctagtt tcaagtctgt ctctacaggg taaatttctc cactatcctt atcccaggct
     7741 cctagtggtc cataaaaact ccgagaagaa gaacctgagg caaatttggc ttcctgtgcc
     7801 aactgacttc tatccaatcc aagcttgaaa taagcattac aagccttgac cagggcggac
     7861 aaaccactag aacttgagga cagacccgct gccgtaggca tattgttttg agtatcgata
     7921 cggacaaagc cctcaccagc tggacgataa cggtcaataa tcttactcat cttggcatgc
     7981 tcgacctcat tttgtagctg accattgatg taaaattcgt cagctgttac attggctggt
     8041 aaaggcgaca aggtcgtctc tgtatacata ttttccaaag ttagagaaat actgctagta
     8101 gcaggcacca tctctttttc ttttttcttt ccccaatatt tgataatagc aatatttgcg
     8161 taggaacgta ctgttacagg ctctctatcc atgtctgaac agctcctttc tcttctaatc
     8221 tttctgctag ttcttgtgcg tgtgtcaaat tggttaccaa ggctatgata caacctccta
     8281 gcccaccacc gctcatcttg gcacccagag caccatggct aagagtcgtt tcaaccaaaa
     8341 agtctgcctc agggctactg actccaattt cttttaaatg taaatgcgct tgactgagga
     8401 tttgtcccag tccttcagca tctttttgtg aaatcgcaac ttctgcttgc tgggttaatt
     8461 ctcccaaggc atgcaaaaac ggtagggcat ccttgccctt attttgaacc acttggatgg
     8521 cttcacgagt atgaccataa acacccgtat cggcaatcac caaataggcg gataaatcca
     8581 tctcaagttc tgtaaatcct acgttcttga taaagcgaat aggttggtca ctaagacagg
     8641 tcttagcatc caaaccacta ggattcatat gggcaatcat ttcagctcga ttgaccaaga
     8701 tttctagtac atcatgaggc agatcagcct gatagtagtc aaatactgca cgaatggccg
     8761 ctatgctgat agccgctgac gaacccatcc cccgtttctc agggatagcc gagtcaatct
     8821 cacaacgaat gcaggcttct gtgatattca aatactccag tgaggcataa accgccatgg
     8881 acaaggtatc ctcctcataa aggcgccaag gactctctgc aggaactacc ttacaggtca
     8941 cctccacctc caaaagaggc agggaaatgg caggataacc gtaaacgacc gcatgttccc
     9001 ctattaaaat tatcttacta tgtgcctgac cgacaccaac tttttttgtc attttttcct
     9061 tttactagac gaaaaaacgt cttatttttc atacaagtat taattctttc ctatctattt
     9121 tattatattt tcacaaaaaa agcgattgtt tccattcaca atcgcttctt tcattattga
     9181 acccattcgc cattatagtt gacagaatag ccatctacgg tcgtattcac tgccaaggca
     9241 cctgagcgct ataagcgtag taccatctgc cattgacctg gaaccaacct gtcgtcatag
     9301 aacgacgaaa gaaactccat accattaagt aaagaggaaa gtcgtgaggg agcatgcgcc
     9361 attgacaacc tgttttagtg acgtacaaag tctcattaac aagtactcgt ttcggccatt
     9421 tataggtgcg gtgtttggag aaatagggtt caatcttcgc ccattcttga tcgtttaaat
     9481 cagtatcata tgctttgcgt atcataactc tagcttaaca tttttttgtg aatacaggtt
     9541 ctaaataatc gaccacgaaa atttcttaag tggaaaacgc cttatgaagt atgctacggg
     9601 aaacttatgc acttaatttg acaattcaag atgtaaaaat atata
//
LOCUS       DAVHIT010000076         9611 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVHIT010000076 DAVHIT010000000
VERSION     DAVHIT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.077"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            229..324
                     /locus_tag="VVN16_001657"
                     /pseudo
     CDS             229..324
                     /locus_tag="VVN16_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(577..1197)
                     /locus_tag="VVN16_001658"
     CDS             complement(577..1197)
                     /locus_tag="VVN16_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5572996.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1197..1679)
                     /locus_tag="VVN16_001659"
     CDS             complement(1197..1679)
                     /locus_tag="VVN16_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU5572997.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1679..2521)
                     /locus_tag="VVN16_001660"
     CDS             complement(1679..2521)
                     /locus_tag="VVN16_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU5572998.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2616..3404)
                     /locus_tag="VVN16_001661"
     CDS             complement(2616..3404)
                     /locus_tag="VVN16_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU5572999.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3548..4927
                     /gene="glmU"
                     /locus_tag="VVN16_001662"
     CDS             3548..4927
                     /gene="glmU"
                     /locus_tag="VVN16_001662"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU5573000.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLTGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4937..5482
                     /locus_tag="VVN16_001663"
     CDS             4937..5482
                     /locus_tag="VVN16_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5573001.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5494..5808
                     /locus_tag="VVN16_001664"
     CDS             5494..5808
                     /locus_tag="VVN16_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU5573002.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5825..6517
                     /locus_tag="VVN16_001665"
     CDS             5825..6517
                     /locus_tag="VVN16_001665"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU5573003.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYSYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHFGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6594..7085
                     /gene="rocS"
                     /locus_tag="VVN16_001666"
     CDS             6594..7085
                     /gene="rocS"
                     /locus_tag="VVN16_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU5573004.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7183..7773
                     /locus_tag="VVN16_001667"
     CDS             7183..7773
                     /locus_tag="VVN16_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5573005.1"
                     /translation="MERLEDYIAFDLEFNQHEGQKHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     YGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7782..9545
                     /locus_tag="VVN16_001668"
     CDS             7782..9545
                     /locus_tag="VVN16_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5573006.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2981 a         1667 c         2050 g         2913 t
ORIGIN      
        1 agttcatgta cacctgatat gatgcgtttt ataattttaa agactttttg accagcctca
       61 tttttttaac ttgatactca gtgaaaagca aagattaaac taggaagcta gctgtaggct
      121 gctcaaagaa cagctttgag gttgcagata aaacttgtga ggtaaccaac atatataatg
      181 tgaagctgac gtggtttgaa tagattttag aagggtatga gtctggaagt tttaatggat
      241 aatgcaagat tccatagaat gggtaagcta gagttcttat gtgaagagtt tgggcataaa
      301 cttttacctt ttcctcccta ctcatcttag tatagaaaag tgaatctaaa atagtacata
      361 actgcttcta aaacattctt ataaattgat ttaaattctc aaatcatatt attcagttct
      421 tatttcattt tgctctacaa tcctgttgag aagacacgtg ttcatatcaa aaaggtattg
      481 gcaagttgca ataccttttt acaaggttct tttgtcttat ttttgtttca actgactata
      541 tctcctatgg ttctagttca gaaggctagg ctataattat gattgataag aagtatcatt
      601 ccaagtattg agagtgaatg tttcaaaatc atgggtttct ataatggtca ggctggcatt
      661 tgctagaccg ccatctttac gaagaagtgg ttctttatag cctaggagag tacgaagact
      721 ggcagtaaga ttggcgccgt gtccgacaat tagaatacgc tcagctggac tatcttttaa
      781 tgatttgata aattggatgg tccgctgagt tgtactatag agggattcgg ctccgaacat
      841 tcgagtgtca aattgagcaa gatttgaacg aaaagcctgg atttgttgcg ggtaaatagc
      901 ttccagggtt gcaattttca aaccttctaa cttcccaagt tgccattcac ggagattagg
      961 aacgatttct aaagaacagg gggtatggag ttgactttgg ataatctcag cagatttgac
     1021 cgctcgaggt aaatcacttg aataaatctg atcaaaagga atttccttga gatactggcc
     1081 aagtcgtttt agggtttcaa tggattcagg aagaagagga gaatcaccgc tagcaccttg
     1141 aaaacgacct tcttggttcc agagggtacg accgtggcgg acaaagtaga gtttcattac
     1201 ttgatgtcct ccaaaatatc tacaaagtct gcctttacaa agctagccaa gtcttgtggc
     1261 gcgacgataa tgctgtgtcc gacttcgcct gcagagacaa tcattcgatc caaatctaga
     1321 gcaattttat cgataaaaat gggataattg tgtttctgac gaattccgac aggattattg
     1381 gctccatgaa tgtaaccagt tgttttttct aagtcctttt gtggaatcat gctcactttt
     1441 ttattgccag aaattttagc tagtttcttt tcagacaagt gctgagtgat agggacaatt
     1501 ccgataatcg gtccggtctt gtctcccaaa agcgccaagg ttttgaaaat ctgatctcgt
     1561 tcataacctt gaggaagctc tccttctagg gcattgattt gaatcccctg atgagggata
     1621 gctgctttag ataggatttg ttccaccaat gtttttttga ttttaacttt ttttgccatt
     1681 atttatattt atcctccaat tgactcatcc aaataccaag ccagattccc agcgcaaaga
     1741 agaaggcgat gatgacataa ccgacaagtg aaagtcctgt gtattggata ctttcagcgt
     1801 ttcctgcatt tggaattaag atcaaaaggg tacttgatag gacgataccg atgatgaaat
     1861 gatagacgcg tgagtggtag ttgtttaagg cataatccat caattttgaa aaaacgatga
     1921 gagttgcacc tgcaccaatt ccaatcggaa agaaggttcc caagaaatca aaagttttaa
     1981 aaccagtcaa cataggagca tatagtccca aaatcaaaag taaatttgat gggctgaggc
     2041 caggaaccaa gacgccaagg gccaatagtg cgcctgctag gacgaagtta agaaagctgg
     2101 cgcttaaggt tccaacgaca aaatttaagg catagagtcc taatccagaa atgataaagg
     2161 ttgtccataa ccaagctaaa tcaatcttgt ctcggtcaga ttctcgagtt gattctttga
     2221 ggaggctagg aactgtaccg ataatagctc ccgcaaagct ccataataca aaaacctgat
     2281 aattttcaag caggtattca atcgggtagg aaaataagcc gattcccaga agcataccga
     2341 tggcaactgg aataaagtac aaaacatttt ctttaaagtc tttaaaggga tgggccagaa
     2401 agccaatcat tcgttcatag attcctaaga ttgctgctag aacccctccg gaaattcccg
     2461 gtaggataaa tccaagagca attacaatcc ctttaataac gcgtgctaac catgagagca
     2521 tatatttcct ccaactattt ctatttcaaa aaatccttac tatattgtat cacaatcaga
     2581 cacaaaaaga aaaagcaaat gataaacaaa tgcttttaag gttttaaaaa aagttttcga
     2641 aaggtttctt ctttattttt taagggagag ataacgttga tatctaaatc gtggtcaaag
     2701 ccggcaattt ttcctttaga tgtgtattgg tgaatatcat aatctaaatc agttttagga
     2761 ctgctctcca aaaatcctga gtctgagccg taggacggaa tccaaacaga ggtaaacttg
     2821 cctgtatcaa tactgtgttc ttccatgaag tagacaccaa cgtagatgcc gatgttttta
     2881 gcacctagtg atgctagttt tgctcgaaag ttttcgacac cttcgttcat attagacatg
     2941 gttttgtctt ccacgtcaag ccaatagtaa ctagggctgt aaggagaggc agcattgtag
     3001 aaaacttcgg cagccttttc catttcttgg acactttttc cagctacata agcgtagaca
     3061 gcaactggga cattccgctt ttgaagttca gtgatatgac tcttataggc cttgtctatt
     3121 ccattgataa atgaagcatc attttctttt gttgtttgag caccactgtg aacacgaaca
     3181 atagcacctg aaatattttg tgagagggca tcgtagttga tttcctcagg acgctgccag
     3241 ccagagaggt caataatcgg tttgtctaag tgtttcaaag cctgtgctat attggatttt
     3301 gttttaaacg attggctgtc attaagtggg cgattgatga ttaaaatgaa catcataatc
     3361 ccaaaaaaac taaataaaat aagtggatga atttgttttc tcatatctta taagtctacc
     3421 ctaaaaatca aaaaaaatca aaaaaatggg ttaaggaaga gactttagag cattttttca
     3481 ttcaagagtg cggaatgatt tgaaatatgg tataataaaa gggaatttct acagaaaaga
     3541 gaagattatg tcaaattttg ccattatttt agcagcgggt aaagggactc gcatgaaatc
     3601 tgatttgcca aaagttttgc acaaggttgc gggtatttct atgttggaac atgttttccg
     3661 tagtgtggga gctatccaac ctgaaaagac agtaacagtt gtaggacaca aggcagaatt
     3721 ggttgaggag gtcttgactg gacagacaga atttgtgact caatctgaac agttgggaac
     3781 tggtcatgca gttatgatga cagaacctat cttagaaggt ttgtcaggac acaccttggt
     3841 cattgcagga gatactcctt taatcactgg tgaaagcttg aaaaacttga ttgatttcca
     3901 tatcaatcat aaaaatgtgg ccactatctt gactgctgaa acggataatc cttttggcta
     3961 tggacgaatt gttcgtaatg acaatgctga ggttcttcgt attgttgagc agaaggatgc
     4021 tacagatttt gaaaagcaaa tcaaggaaat caacactgga acatacgtct ttgacaacga
     4081 gcgtttgttt gaggctttga aaaatatcaa taccaataac gctcaaggcg aatactatat
     4141 tacagacgtc attggtattt tccgtgaaac tggtgaaaaa gttggcgctt atactttgaa
     4201 agattttgat gaaagtcttg gggtaaatga ccgtgtggcg cttgcgacag ctgagtcagt
     4261 tatgcgtcgt cgcatcaatc ataaacacat ggtcaacggt gttagctttg tcaatccaga
     4321 agcaacttat atcgatattg atgttgagat tgctccggaa gttcaaatcg aagccaatgt
     4381 taccttgaaa gggcaaacga aaattggtgc tgagactgtt ttgacaaatg gtacttatgt
     4441 agtggacagc actatcggag caggagcggt cattaccaat tctatgattg aggaaagtag
     4501 tgttgcagac ggtgtgacag tcggtcctta tgctcacatt cgtccaaatt caagtctggg
     4561 tgcccaagtt catattggta actttgttga ggtgaaagga tcttcaatcg gtgagaatac
     4621 caaggctggt catttgactt atatcggaaa ctgtgaagtg ggaagcaacg ttaatttcgg
     4681 tgctggaact attacagtca actatgacgg caaaaacaaa tacaagacag tcattggaga
     4741 caatgtcttt gttggttcaa attcaaccat tattgcacca gtagaacttg gtgacaattc
     4801 cctcgttggt gctggttcaa ctattactaa agacgtgcca gcagatgcta ttgctattgg
     4861 tcgcggtcgt cagatcaata aagacgaata tgcaacacgt cttcctcatc atcctaagaa
     4921 ccagtaggag cctatcatgg agtttgaaga aaaaacgctt agccgaaaag aaatctatca
     4981 aggaccaata tttaaactgg tccaagatca ggttgaatta ccagaaggca agggaactgc
     5041 ccaacgggat ttgattttcc acaatggggc tgtctgtgtt ttagcagtaa cggatgaaca
     5101 aaaacttatc ttggtcaagc agtaccgcaa agctatcgag gcagtctctt acgaaattcc
     5161 agccggaaaa ttggaagtag gagaaaacac agcccctgtg gcagctgccc ttcgtgaatt
     5221 agaggaagaa acagcctata cagggaaatt agaactcttg tacgattttt attcagctat
     5281 tggcttttgt aatgagaagt taaaactata tttagcaagc gatttaacaa aagtggaaaa
     5341 tccgcgtccg caggatgagg atgaaacctt ggaagtcctt gaagtgagct tagaagaagc
     5401 gaaagaatta atccaatcag gtcatatttg tgatgccaag acaattatgg ctgttcagta
     5461 ttgggagttg cagaaaaaat agaggaggtc agtatgggta aatctttatt aacggatgaa
     5521 atgattgaaa gagctaatag aggcgaaaaa atttcaggtc ctcctttgct agatgataat
     5581 gaggaaacta agattttacc aacctcttct tcccgttttg gttatgccaa tcctaaggat
     5641 catggtttta gccaggaaac cttgaagatt caggtcgaac catctattca taaaagccgt
     5701 cgtattgaaa ataccaagag aaatgtcttc aattctaagt tgaataaaat cttatttgcg
     5761 gtcatttttc tcttgatttt gcttgtttta gcaatgaaac ttttgtaata gaaaaggaat
     5821 tgaaatgaaa ataggaatta ttgctgctat gccagaagaa ctggcttatc tggtccagca
     5881 tttagataat gcccaggagc aagttgtttt ggggaatacc tatcatacag gaaccattgc
     5941 ttctcatgaa gtcgttcttg tagaaagtgg aattggtaag gtcatgtctg ctatgagtgt
     6001 ggcgattttg gctgatcatt tccaggtgga tgcccttatt aatacgggtt cagctggggc
     6061 agtagcagaa ggtatcgctg ttggggatgt cgtgattgct gacaaattag cctatcatga
     6121 cgtggatgtc acagcttttg gctattctta tggacaaatg gcgcaacaac cgctttattt
     6181 cgaatcagac aaaacctttg ttgctcaaat ccaagagagt ttatctcaat tggaccaaaa
     6241 ctggcatttt ggtttgattg ctacaggaga tagttttgtt gcaggaaatg acaagataga
     6301 agcgattaag tcccatttcc cagaagtttt agccgtggag atggaggggg cagctattgc
     6361 tcaagcagcg catgccctca atctcccagt cttagtcatc agagctatga gtgacaatgc
     6421 caaccatgaa gcaaacatct tttttgatga gtttattatc gaagctggac gtcgctctgc
     6481 ccaagtcttg ttgacctttt tgaaggcttt agattaagcg gaaatttgac agtttttcta
     6541 gcttatgata agatttaagt aaagaaaagc tagaaaacgt ttcagaggat attatgagta
     6601 ttgaaatgac cgtcagtgag attgcagagg tcttaggatt atctcgccaa gcaatcaata
     6661 accgtgtcaa agaattacca gaagaagaca cagataaaaa tgacaaaggg gtaacagttg
     6721 ttaccagaag tggcttgatt aagctagaag aaatctataa aaaaacgatt tttgaagatg
     6781 agcctgtcag tgaagatgtc aaacaacgtg aactgatgga gattctagtg gatgagaaga
     6841 atgcagaaat tcttcgtctt tatgaacaat taaaagccaa ggatcgtcag ttatcagaaa
     6901 aagacgagca gatgcgtatc aaagaccgtc agattgctga gaaagacaaa caattagatc
     6961 agcaacaaca attgaccctt caagccatga aggatcaaga aaatcttaag ctagagctgg
     7021 accaagcaaa agaagaagtc caatccacta aaaaaggctt ttttgctcgt ttatttggag
     7081 gataatcaag gagctgttta ggttaaatgc taacctgatg gcttcttttg tttgtgaata
     7141 gtatagttga ttagaacaga gacagtagag gagaggtaca gaatggaacg attagaagat
     7201 tacattgctt ttgacttaga atttaatcag cacgaaggac aaaaacactt gattcaagta
     7261 tcggcagtgc gttttagaga tggtcgggaa atcgatgcct atgattccta tgttcatacc
     7321 agcgtccctc taaaaagctt tatcaatggt ttgaccggga ttacagctga gaccttaaaa
     7381 gatgctcctc cagttgagaa agtcttagaa gaattccaag agtttgtggg ttctttgctg
     7441 atgataggct acaatgctgc taaaagtgat ttacctattt tagcagagta tggtttagac
     7501 tatagccaac agtacaaggt ggatttgtat gatgaagctt ttgaacgtcg gagttcagat
     7561 ttacatggta ttgccaatct caaattgcaa actgttgcct catttcttgg atttcaaggt
     7621 cagtctcata atagtttaga ggatgctcgg atgacggcgc gtgtttacga atccttctta
     7681 gagtcggatc aagctaggga attgttagag acagaaagta gcttatcaaa caatcctttt
     7741 ggtggcttgg acttgtctca attatttgac taggagtgcc tatgaaacct gaaaaaccta
     7801 aaaaattggg tcttaaacag atttacctca atcttgataa aatcctcttt ctatttttct
     7861 tgatttttat gatagtggag tatttgtggg taccattcaa ttcatggttg gctagcttct
     7921 tattagatca gacaggttat ctttttattt cctataacaa tgtcttagcc atccttactc
     7981 attctccatt gattagtcta gcttttttag ccttgattgt aattaacctt ctggttgctt
     8041 attttcaaat aggactcctc tttattggtg ctcgtcatct cctctataat gaaaagcgaa
     8101 ggctaattga gtatatccgc aaggttttcc atgaaagttt tggatttatg aaaaagctaa
     8161 cttgcgctaa agccttgttt atctttttct atatagctat gctttttcct tttatacgga
     8221 aaatgctgaa gatttattat cttaataaaa tcgtcattcc agagtttatc caagcttatt
     8281 tagaagataa atattggatg tggtggctga gcatcctact tctatctctt atctttctct
     8341 atgtctctgt cagattgatg tttgcccttc cgaagattgt atatgatcag ctgactgttc
     8401 gagaagcagt gatgtttagc ttggagaaaa ccaagaagag agttactttt tatgcttgga
     8461 atttatttta catcttgctc aaagcacatt tattatttta tcttcctttg attcctttat
     8521 tattggctca aactttggta gatgatatca ctcagagaga gtctctgatt cttggtattt
     8581 tgaattttgt cgtgattaaa aatctctatt atatggctct gacttatttt ttggttaaat
     8641 ttgtttcatt tttgacagga aaggagctgg atatgcttcc taggagagaa aaagatcaca
     8701 tcgtgcgatg gggtgtcatg acttgtgcca gtcttttctt tgccttagaa ggttatattt
     8761 atctggaggc tcccatggtt catctacctc aacttatttc tcaccgaggg gtttccaatg
     8821 caaatgggat tcaaaataca gtagagtcct tggaaactac agcacaactc aaaccagact
     8881 tggtggagac ggacgtgcag gaaacaaaag atgggcagtt tgtcatgatg catgatgcta
     8941 acttgaaaaa tctagcaggt atcaataaaa gtcctcaaga cttaaacttg gaggagctta
     9001 aagggattga tatttttgaa aatggctacc agactaaaat ttcaagcttt gaagattatc
     9061 tcagtcgagc caacgaactt ggtcaaaaat tactaattga aattaaaacc agtaaaaaag
     9121 atagtccaga catgatgaac cgctttttag cccgttatgc tgcaaagctc aagatttatg
     9181 gacatcaaat ccagtcttta gactaccatg ttgtcgaaaa agtaagacag tatgatgccg
     9241 aactgccagt ttatttcatc atgccctaca attctgtctt tcctaaaaca agagcgacag
     9301 gatatactat ggagtactca accttggatg aatattttgt aagcaaacta tggacaacgg
     9361 atcagaaact ttatgtctgg actgtcaatg attcagaagc tatcagcaaa tctcttcact
     9421 taggagtaga tggagtgatt acagatgatt tggaaaaagt tcagcgggaa atagaagtag
     9481 cccaagaaga tccagaatat acggatttgc tcttgaaaaa agcattggaa ttctttgaat
     9541 tttagtaata aaaaagagga acggttgttc ctcccatgat aggtaagtat tcttgtgaat
     9601 gcttgctttt t
//
LOCUS       DAVHIT010000077         9523 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVHIT010000077 DAVHIT010000000
VERSION     DAVHIT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.078"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            44..1717
                     /locus_tag="VVN16_001669"
     CDS             44..1717
                     /locus_tag="VVN16_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU5573007.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            1829..2920
                     /gene="ulaG"
                     /locus_tag="VVN16_001670"
     CDS             1829..2920
                     /gene="ulaG"
                     /locus_tag="VVN16_001670"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU5573008.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            3034..5010
                     /gene="tkt"
                     /locus_tag="VVN16_001671"
     CDS             3034..5010
                     /gene="tkt"
                     /locus_tag="VVN16_001671"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5573009.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNFAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            5127..5426
                     /gene="yajC"
                     /locus_tag="VVN16_001672"
     CDS             5127..5426
                     /gene="yajC"
                     /locus_tag="VVN16_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU5573010.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            5472..5900
                     /locus_tag="VVN16_001673"
     CDS             5472..5900
                     /locus_tag="VVN16_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU5573011.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            5902..6312
                     /locus_tag="VVN16_001674"
     CDS             5902..6312
                     /locus_tag="VVN16_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573012.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            6612..9263
                     /gene="adhE"
                     /locus_tag="VVN16_001675"
                     /gene_synonym="adhC"
     CDS             6612..9263
                     /gene="adhE"
                     /locus_tag="VVN16_001675"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU5573013.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         3036 a         1762 c         1990 g         2735 t
ORIGIN      
        1 aaagaattgc tgtgatatca atagatttgg gggatttttt aatatggtac tggataaggc
       61 aagttgtgat ttgcttcaat atttgatgga tcaagaaacg tccaaaacga ttatggcgat
      121 ttcgaaagat ttgaaagagt caagaaggaa aatttattat cacattgaca aaatcaatgc
      181 tgctctggtt gacgaggcgc ttcacatcat tagtattcca cgaattggta ttcacttaac
      241 ggaagagcag agagatgctt gttgtaaact attatcggaa gtagattcgt acgattatat
      301 catgagtgcg catgaacgta tgatgataat gttactatgg ataggtattt ctaaagaacg
      361 tattacgatt gaaaaattga tagagttaac agaggtatct aggaatactg ttctcaatga
      421 tttgaatagt attcgttatc aactaacttt ggaacaatat caggtgatct tgcaagtgag
      481 caagtcacag ggataccacc ttcatgccca ccctcttaat aaaattcagt atcttcaatc
      541 gcttctatat catattttta tggaagaaaa tgccactttt gtatctattt tagaagataa
      601 gatgaaagag aggttagatg atgagtgttt gctttctgtt gaaatgaacc aattttttaa
      661 ggaacaggtt cctttagttg aacaagattt agggaagaaa ataaaccatc atgaaataac
      721 ttttatgttg caggttctac cttatttgct gttaagctgt cataatgttg aacagtatca
      781 agaaagacat caggatatag agaaagaatt ttctttgata agaaaaagaa tagagtatca
      841 ggtgtctaag aaattaggag aacggttgtt tcaaaagttt gaaatttctt tgtcaggact
      901 tgaagtttct cttgtagctg ttctcctcct ctcctatcgt aaagatttgg atattcatgc
      961 agaaagtgat gattttcggc aattaaaact tgctttagaa gaatttatct ggtattttga
     1021 atcacaaatc cgaatggaga ttgagaacaa ggatgatttg ttacgaaatt tgatgatcca
     1081 ctgtaaagcc ttgttattta gaaagactta cggtattttt tctaaaaatc ctctaacaaa
     1141 acaaattcga tccaagtatg gagaattatt tttagtcact agaaaatctg cggaaatttt
     1201 agaaggagca tggtttattc ggctaacaga cgatgatatt gcctatttga cgattcatat
     1261 tggaggattt ttaaagtata caccatcgtc tcaaaaaaat atgaaaaaag tttatctcgt
     1321 ttgtgatgaa ggtgttgcgg tttcgagact tttgctgaaa caatgcaaac tttattttcc
     1381 aaatgagcaa attgacactg tatttacaac agaacaattt aagagtgtgg aagatattgc
     1441 acaagttgat gtagtgatta ctactaatga tgatttggat agcagatttc cgattttaag
     1501 ggttaatcct atccttgaag cagaagatat tttgaaaatg ctagactatc ttaaacacaa
     1561 tatatttcgt aataagagca aaagtttcag tgaaaatctt tctagtctta tttcgtctta
     1621 tattgtagac agcaagttgg ctagtaagtt ccaagaagag gttcaaacac ttataaatca
     1681 agaaatagta gttcaagctt ttttggaaga tatttgaagg acagtccaat gatgaacaca
     1741 aacctgtgtt tttcttggtc ttttttagtg ctttgaaggg tggtatacta atctcaaaga
     1801 taacaattat atccaaagga ggcaacatat gccaaacgtc aaagaaatta caagagagtc
     1861 atggatttta gccactttcc cagagtgggg aacatggttg aacgaagaaa tcgaagaaga
     1921 agtcgtacct gaaggcaact ttgccatgtg gtggctaggc aactgtggta cttggattaa
     1981 gacaccagct ggtgctaacg ttgtcatgga cctttggtca aaccgtggaa aatcaaccaa
     2041 aaaagtgaaa gatatggttc gtgggcacca aatggcaaat atggcaggtg ttcgtaagtt
     2101 gcaaccaaac ttgcgtgttc agccaatggt tatcgatcca tttgctatca acgaactaga
     2161 ctattactta gtttcacact tccacagtga tcatatcgac ccatacacag ctgcagcaat
     2221 tctcaacaat cctaagttag agcatgttaa gtttatcggt ccttaccact gtggacgaat
     2281 ctgggaagga tggggggttc caaaagaacg tatcatcgtt gttaaaccag gtgacactat
     2341 cgaattaaaa gatatgaaga ttcatgcagt agaatcattt gaccgtactt gcttggtaac
     2401 tctcccagtg aacggtgctg atgagacagg cggtgaactt gctggcttgg ctgttacaga
     2461 tgaagaaatg gctcaaaagg ctgttaacta tatctttgaa acaccaggtg gaaccatcta
     2521 tcatggtgca gattctcact tctcaaacta ttttgcaaaa catggtaaag actttaaaat
     2581 tgatgttgct ttgaataact atggtgaaaa tccggtaggt atccaagaca aaatgacatc
     2641 tatcgacctt cttcgtatgg cagaaaatct gcgtaccaaa gtcattatcc cagttcacta
     2701 tgatatctgg tctaacttca tggcttctac taatgagatt ctagaacttt ggaaaatgcg
     2761 aaaagatcgc ttgcaatacg atttccatcc atttatctgg gaagttggcg gtaagtacac
     2821 ttatcctcaa gatcaacact tagtagaata ccatcatcca cgtggttttg atgattgttt
     2881 tgaacaagac tctaacattc aatttaaagc tttgctataa tataaaaata ttctttggag
     2941 gttatccgta tgttgcatga tacggataat tttcatataa tagtaaaata gaatgtgtga
     3001 ttcaataatc acctcaaata gaaaggaaat tctatgtcaa atctatctgt taatgcaatt
     3061 cgttttctag gtattgacgc cattaataaa gccaactcag gtcatccagg tgtggttatg
     3121 ggagcggctc cgatggctta cagcctcttt acaaaacaac ttcatatcaa tccagctcaa
     3181 ccaaattgga ttaaccgcga ccgctttatt ctttcagcag gtcatggttc aatgctcctt
     3241 tatgctcttc ttcacctttc tggttttgaa gatgtcagca tggatgagat taagagcttc
     3301 cgtcaatggg gttcaaaaac accaggtcac ccagaatttg gtcatacggc agggattgat
     3361 gctacgacag gtcctctagg gcaagggatt tcaactgcta ctggttttgc ccaagcagaa
     3421 cgtttcttgg cagccaaata taaccgtgaa ggctacaata tctttgacca ctatacttac
     3481 gttatctgtg gagacggaga cttgatggaa ggtgtctcaa gcgaggcagc ttcatacgca
     3541 ggtttgcaaa aacttgataa gttggttgtt ctttatgatt caaatgatat caacttggat
     3601 ggtgagacaa aggattcctt tacagaaagt gttcgtgacc gttacaatgc ctacggttgg
     3661 catactgcct tggttgaaaa tggaacagac ttggaagcca tccatgctgc tatcgaaaca
     3721 gcaaaagctt caggcaagcc atctttgatt gaagtgaaga cggttattgg atatggttct
     3781 ccaaacaaac aaggaactaa tgctgtacac ggcgcccctc ttggagcaga tgaaactgca
     3841 tcaactcgcc aagccctcgg ttgggactac gaaccatttg aaattccaga acaagtatat
     3901 gctgatttca aagaacatgt tgcagaccgt ggcgcatcag cttatcaagc ttggactaaa
     3961 ttagttgcag attataaaga agctcatcca gaactggctg cagaagtaga agccatcatc
     4021 gacggacgtg atccagtcga agtgactcca gcagacttcc cagctttaga aaatggtttt
     4081 tctcaagcaa ctcgtaattc aagtcaagat gccttgaatg ttgtggcagc taaattacca
     4141 accttcctag gtggatcagc tgaccttgct cactcaaaca tgacttatat caagacggat
     4201 ggacttcaag acgataccaa tcgcttgaat cgtaacattc agtttggtgt tcgtgaattt
     4261 gcaatgggaa caatcttgaa cgggatggcc cttcatggtg gacttcgtgt atacggtgga
     4321 actttcttcg tcttctctga ctatgtgaag gcagctgtcc gcttgtcagc cttacaagga
     4381 cttcctgtga cttatgtctt tacccatgat tcaatcgcag ttggggaaga tggtccgact
     4441 cacgaaccag ttgagcattt agcaggtctt cgtgctatgc caaatctaaa tgttttccgt
     4501 ccagcagatg cgcgtgaaac gcaagcagct tggtaccttg cagtgacaag tgagaaaaca
     4561 ccaactgccc ttgtcttgac acgtcaaaac ttgactgttg aagatggaac agacttcgac
     4621 aaggttgcta aaggtgctta tgttgtatat gaaaatgcag ccgactttga taccatcttg
     4681 attgcgacag gttcagaggt taattttgct gtctcagctg ctaaagaatt ggctagtcaa
     4741 ggcgaaaaaa tccgcgtagt cagcatgcca tctacagatg tctttgataa acaagatgca
     4801 gcttacaagg aagaaattct tccaaatgca gtccgccgtc gtgttgcagt cgaaatgggt
     4861 gcaagtcaaa actggtacaa atatgttggt ctcgatggtg ccgttctagg tattgatacc
     4921 ttcggagcct ctgccccagc accaaaagta ttggcagaat atggctttac tgtcgaaaat
     4981 cttgtaaaag ttgttcgaaa tttgaaataa tcctaaaaat cagggcgtaa gctctggttt
     5041 ttcttaccag aaaagtaagg tacaatcttg taaaagtagc tgaaatttga tatagtagtc
     5101 ctatgtaaaa gacaaaggag aaaacaatga atccaaatat tactttttta atcatgcttg
     5161 taggtatgat ggccttgatg ttctttatgc aacgttctca aaagaaacaa gctcaaaagc
     5221 gtatggaaag cttaaataaa ctacaaaaag gctatgaagt gattacaatc ggtggacttt
     5281 atggaacagt cgatgaagta gatacggaga agggaacaat agttcttgac gtagatggag
     5341 tttacttgac ttttgaacta gctgctatca agacagtatt accgctgaaa gaaacagctt
     5401 cactagaagg cgcaattgaa aaataagacg ggatcacgaa ctcccgtttt ctataaaaga
     5461 aaggaaatgg gatgaaaaaa ttagtctttg tctgtctggg aaatatttgc cgtagcccta
     5521 tggccgagtt tgttatgaaa tcaatgacag ataactacga aatccaaagt cgagcaactt
     5581 cctcttggga acatggcaat ccgattcata aggggactca gggaattttt caagagtatg
     5641 agattcctta tgacaagaac aagacatcgc ttcagattag taaggaagat tttgaagcct
     5701 ttgattatat tatcggaatg gacgcttcaa atatttctga cttacgtcag atgtgtccag
     5761 tagactgtca agataagatt tactcatttt catctgaaag tgttccagac ccttggtata
     5821 caggagattt tgaagaaacc tatcgacgtg ttcaagaggg ctgtcaagct tggttagaac
     5881 gtttagaaaa ggagagttaa ggtggaaaat cttacgaatt tttacgaaaa gtatcgtgtc
     5941 tatctgactc gtccacgttt agagcttttg gcagtagtta ccattgtttt ctgtgctgta
     6001 ctcgtctttt ttctaaatat tccaggaaaa ggtgtcttaa aactcgataa tggaacgatt
     6061 gtttatgatg gcagtcttgt ccgtggtaaa atgaatggcc aaggtaccat taccttccaa
     6121 aatggagacc aatatacagg tggcttcaac aatggagcct tcaacggaaa aggtaccttt
     6181 caatctaaag aaggctggac ctacgaaggt gattttgtaa atggtcaggc tgaaggaaaa
     6241 gggaaactaa caacagaaca agaagtcgtt tatgaaggaa cttttaaaca aggcgttttt
     6301 caacaaaaat aaagcctcct tatcaaagga ggtattatta gaatcacaag gtaagcgctt
     6361 acctgtaaat ccctttcttt ccaaatccct cttccaagca agtttgtgaa ataaaaaata
     6421 tttgaaataa atttcacaaa cttcaaagat aaaacttgat aagaaaagaa aatgagaaaa
     6481 gtttcacaag agtttaaaaa ttctttgtga aatgtttatg aaactgttta caaagttgaa
     6541 ataatgcgtt ataataaact tgtgaaaaaa ttaacaaagg atatcgttcc ttgaaagcta
     6601 tggaggaaaa tatggctgat aaaaaaactg tgacaccaga ggaaaagaaa ctcgttgctg
     6661 aaaaacacgt agatgagttg gttcaaaaag ctctagttgc ccttgaagaa atgcgtaaat
     6721 tggatcaaga acaagttgac tacatcgttg ccaaagcatc agtagcagct ttggatgccc
     6781 acggagaatt ggctttacat gcctttgaag aaacaggacg tggtgtattt gaagacaaag
     6841 caactaagaa cttgtttgcc tgtgaacacg tagtaaacaa catgcgccac actaagacag
     6901 ttggcgttat cgaagaagac gatgtaacag gattgactct tattgctgaa ccagttggtg
     6961 ttgtttgtgg tattactcca acaacaaacc caacatcaac agcaatcttc aaatcattga
     7021 tttcattgaa gacacgtaac ccaatcgtct ttgccttcca tccatcagca caagaatcat
     7081 ctgctcatgc agctcgtatc gtccgcgatg cagctatcgc agctggtgct cctgaaaact
     7141 gtgtgcaatg gattactcaa ccatctatgg aagcaacaag tgcccttatg aaccacgaag
     7201 gtgttgcgac aatccttgca acaggtggta atgccatggt taaggcggct tattcatgtg
     7261 gtaaaccagc tcttggggta ggtgccggaa acgttccagc ttatgttgaa aaatcagcaa
     7321 acattcgtca agcagcacac gatatcgtca tgtctaaatc atttgataac ggtatggtct
     7381 gtgcatctga acaagcagtt atcattgata aagaaattta cgatgaattt gtagcagagt
     7441 tcaaatctta ccacacttac tttgtaaaca aaaaagaaaa agctcttctt gaagagttct
     7501 gcttcggcgt caaagcaaac agcaaaaact gtgctggtgc aaaattgaac gctgacatcg
     7561 ttggtaaacc agcaacttgg attgcagaac aagcaggatt tacagttcca gaaggaacaa
     7621 acattcttgc tgcagaatgt aaagaagttg gcgaaaatga gccattgact cgtgaaaaat
     7681 tgtcaccagt tattgcagtt ttgaaatctg aaagccgtga agatggtatt actaaggctc
     7741 gtcaaatggt tgaatttaac ggtcttggac actcagcagc tatccacaca gctgacgaag
     7801 aattgactaa agaatttggt aaagctgtta aagctattcg tgttatctgt aactcacctt
     7861 ctacttttgg tggtatcggg gacgtttaca atgccttctt gccatcattg acacttggat
     7921 gtggttctta cggacgcaac tcagttgggg ataacgttag tgccattaac ctcttgaata
     7981 tcaaaaaagt cggaagacgg agaaataaca tgcaatggat gaaacttcct tcaaaaacat
     8041 actttgaacg tgattcaatt caataccttc aaaaatgtcg tgacgttgaa cgtgtcatga
     8101 tcgttactga ccatgccatg gtagagcttg gtttccttga tcgtatcatc gaacaactgg
     8161 accttcgtcg caataaggtt gtttaccaaa tctttgcgga tgtagaaccg gatccagata
     8221 tcacaactgt aaaccgtggt actgagatta tgcgtgcctt caaaccagat accatcatcg
     8281 cactcggtgg tgggtctcca atggatgctg ccaaagtaat gtggctcttc tacgagcaac
     8341 cagaagtgga cttccgtgac cttgtccaaa aattcatgga tatccgtaaa cgtgccttca
     8401 agttcccatt gcttggtaag aagactaaat tcatcgcgat tccaactaca tctggtacag
     8461 gatctgaagt aacaccattt gccgttatct ctgataaagc aaacaaccgt aaatacccaa
     8521 tcgctgacta ctcattgaca ccaactgtgg caatcgtaga tcctgctttg gtattgacag
     8581 ttccaggatt tgttgctgct gatactggta tggacgtatt gactcacgcg acagaagcat
     8641 acgtatcaca aatggctagt gactacactg atggtttagc acttcaagcc attaaattgg
     8701 tctttgaaaa tctcgaaagc tcagttaaga atgcagactt ccactcacgt gagaaaatgc
     8761 ataacgcttc aacaatcgct ggtatggcct ttgccaatgc cttcctaggt atttctcact
     8821 caatggccca taagattggt gcgcaattcc acacaatcca cggtcgtaca aatgccatct
     8881 tgcttccata cgttatccgt tacaacggta cacgtccagc taagacagca acatggccta
     8941 agtacaacta ctaccgtgca gatgaaaaat accaagatat cgcacgcatg cttggacttc
     9001 cagcttctac tccagaagaa ggggttgaat cttacgcaaa agctgtctac gaactcggtg
     9061 aacgtattgg gatccaaatg aattttagag accaaggaat tgacgaaaaa gaatggaaag
     9121 aacattctcg tgaattagcc ttcctggctt atgaagacca atgttcacca gctaacccac
     9181 gtcttccaat ggtagaccat atgcaagaaa tcatcgaaga tgcatactat ggctacaaag
     9241 aaagaccagg acgccgtaaa taattgttta tcagtctaga agcaagacaa aaactcaatt
     9301 tgagggaaag aaccagtaat ttttctatga taaaaggcat cctatcaagg tttttgaata
     9361 cctgatagga tgccttttta tgatgttgag gcctttttgc cctttttgaa aaactagaat
     9421 agaaacaaaa tatatagtag gttgaaacta gaatagtaca catctgcttc taaaacattg
     9481 ttagaattcg atttgactgt cctgatcgat ttgtcctgtt ctt
//
LOCUS       DAVHIT010000078         9323 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVHIT010000078 DAVHIT010000000
VERSION     DAVHIT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.080"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            214..2874
                     /gene="rrgA"
                     /locus_tag="VVN16_001676"
     CDS             214..2874
                     /gene="rrgA"
                     /locus_tag="VVN16_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU5573014.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGWTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYERKDESVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAVGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GAPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVFYDTTEKRIRVTGLYLGTGEKVTLTYNVRLNDQFVSN
                     KFYDTNGRTTLHPKEVEKNTVRDFPIPKIRDVRKYPAITIAKEKKLGEIEFIKINKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3097..5094
                     /gene="rrgB"
                     /locus_tag="VVN16_001677"
     CDS             3097..5094
                     /gene="rrgB"
                     /locus_tag="VVN16_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HEU5573015.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            5141..6322
                     /gene="rrgC"
                     /locus_tag="VVN16_001678"
     CDS             5141..6322
                     /gene="rrgC"
                     /locus_tag="VVN16_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU5573016.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKTN
                     N"
     gene            6666..7562
                     /gene="srtC-1"
                     /locus_tag="VVN16_001679"
     CDS             6666..7562
                     /gene="srtC-1"
                     /locus_tag="VVN16_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU5573017.1"
                     /translation="MAKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPAIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     RQSERALKALKEATKEVKVEDE"
     gene            7555..8448
                     /gene="srtC-2"
                     /locus_tag="VVN16_001680"
     CDS             7555..8448
                     /gene="srtC-2"
                     /locus_tag="VVN16_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU5573018.1"
                     /translation="MSKSRYSRKKSVKKKKNPFILLLIFLVGLAVAMYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTDQEKKQGVSEYAN
                     MLKVHERIGYVEIPAIEQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGQDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            8435..9286
                     /gene="srtC-3"
                     /locus_tag="VVN16_001681"
     CDS             8435..9286
                     /gene="srtC-3"
                     /locus_tag="VVN16_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEU5573019.1"
                     /translation="MSRTKLRVLLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
BASE COUNT         3018 a         1540 c         2185 g         2580 t
ORIGIN      
        1 ttgtatacaa gtgtgtcatt gccaggttga gaagatagct ataacgcact tttatacgct
       61 tttgctacgt ttgttagtga acggattaac tcagtgagat aaattttatc agaacataag
      121 taatccgttt cttcgtgtat acagattgaa agtacctatg aatcatagaa ggattaactt
      181 gttctatgaa taatgcttaa cagggagaca cacatgaaaa aagtaagaaa gatatttcag
      241 aaggcagttg caggactgtg ctgtatatct cagttgacag ctttttcttc gatagttgct
      301 ttagcagaaa cgcctgaaac cagtccagcg ataggaaaag tagtgattaa ggagacaggc
      361 gaaggaggag cgcttctagg agatgccgtc tttgagttga aaaacaatac ggatggcaca
      421 actgtttcgc aaaggacaga ggcgcaaaca ggagaagcga tattttcaaa cataaaacct
      481 gggacataca ccttgacaga agcccaacct ccagttggtt ataaaccctc tactaaacaa
      541 tggactgttg aagttgagaa gaatggttgg acgactgtcc aaggtgaaca ggtagaaaat
      601 cgagaagagg ctctatctga ccagtatcca caaacaggga cttatccaga tgttcaaaca
      661 ccttatcaga ttattaaggt agatggttcg gaaaaaaacg gacagcacaa ggcgttgaat
      721 ccgaatccat atgaacgtgt gattccagaa ggtacacttt caaagagaat ttatcaagtg
      781 aataatttgg atgataacca atatggaatc gaattgacgg ttagtgggaa aacagtgtat
      841 gaacgaaaag atgagtctgt gccgctggat gtcgttatct tgctcgataa ctcaaatagt
      901 atgagtaaca ttcgaaacaa gaatgctcga cgtgcggaaa gagctggtga ggcgacacgt
      961 tctcttattg ataaaattac atctgatcca gaaaataggg tagcgcttgt gacttatgct
     1021 tccactatct ttgatgggac cgagtttaca gtagaaaaag gggtagcaga taaaaacgga
     1081 aaacgattga atgattctct tttttggaat tatgatcaga cgagttttac aaccaatacc
     1141 aaagattata gttatttaaa gctgactaat gataagaatg acattgtaga attaaaaaat
     1201 aaggtaccta ccgaggcaga agaccatgat ggaaatagat tgatgtacca attcggtgcc
     1261 acttttactc agaaagcttt gatgaaggca gatgagattt tgacacaaca agcgagacaa
     1321 aatagtcaaa aagtcatttt ccatattacg gatggtgtcc caactatgtc gtatccgatt
     1381 aattttaatc atgctacgtt tgctccatca tatcaaaatc aactaaatgc attttttagt
     1441 aaatctccta ataaagatgg aatactatta agtgatttta ttacgcaagc aactagtgga
     1501 gaacatacaa ttgtacgcgg agatgggcaa agttaccaga tgtttacaga taagacagtt
     1561 tatgaaaaag gtgctcctgc agctttccca gttaaacctg aaaaatattc tgaaatgaag
     1621 gcggttggtt atgcagttat aggcgatcca attaatggtg gatatatttg gcttaattgg
     1681 agagagagta ttctggctta tccgtttaat tctaatactg ctaaaattac caatcatggt
     1741 gcccctacaa gatggtacta taacgggaat attgctcctg atgggtatga tgtctttacg
     1801 gtaggtattg gtattaacgg agatcctggt acggatgaag caacggctac tagttttatg
     1861 caaagtattt ctagtaaacc tgaaaactat accaatgtta ctgacacgac aaaaatattg
     1921 gaacagttga atcgttattt ccacaccatc gtaactgaaa agaaatcaat tgagaatggt
     1981 acgattacag atccgatggg tgagttaatt gatttgcaat tgggcacaga tggaagattt
     2041 gatccagcag attacacttt aactgcaaac gatggtagtc gcttggagaa tggacaagct
     2101 gtaggtggtc cacaaaatga tggtggcttg ctaaaaaatg caaaagtgtt ctatgatacg
     2161 actgagaaaa ggattcgtgt aacaggtttg taccttggaa cgggtgaaaa agttacattg
     2221 acttataatg ttcgcttgaa tgaccaattt gtaagcaata aattctatga cacgaatggt
     2281 cgaacaaccc tacaccctaa ggaagtagaa aagaacacag tgcgcgactt cccgattcct
     2341 aagattcgtg atgtgcgaaa atatccagca attacgattg caaaagagaa aaaacttggt
     2401 gaaattgagt ttattaagat caataagaat gataaaaaac cactgagaga tgcggtcttt
     2461 agtcttcaaa aacaacatcc ggattatcca gatatttatg gagctattga tcaaaatggc
     2521 acttatcaaa atgtgagaac aggtgaagat ggtaagttga cctttaaaaa tctgtcagat
     2581 gggaaatatc gattatttga aaattctgaa ccagctggtt ataaacccgt tcaaaataag
     2641 cctatcgttg ccttccaaat agtaaatgga gaagtcagag atgtgacttc aatcgttcca
     2701 caagatatac cagcgggtta cgagtttacg aatgataagc actatattac caatgaacct
     2761 attcctccaa agagagaata tcctcgaact ggtggtatcg gaatgttgcc attctatctg
     2821 ataggttgca tgatgatggg aggagttcta ttatacacac ggaaacatcc gtaaagtgta
     2881 gaaatgataa tatctatgtt ctgaacgata cttttaagaa gtagcactca agaagagatt
     2941 taagtttact tggtgaaacc agttttcttc gccaagtaaa ccaccattga aaggggagat
     3001 gttttcgaaa acttgcacag aaaaaggatt attattgtca tgtgtaattc attacattgc
     3061 tcacagttga ttttaagaga taaataagga gaaatcatga aatcaatcaa caaattttta
     3121 acaatgcttg ctgccttatt actgacagcg agtagcctgt tttcagctgc aacagttttt
     3181 gcggctggga cgacaacaac atctgttacc gttcataaac tattggcaac agatggggat
     3241 atggataaaa ttgcaaatga gttagaaaca ggtaactatg ctggtaataa agtgggtgtt
     3301 ctacctgcaa atgcaaaaga aattgccggt gttatgttcg tttggacaaa tactaataat
     3361 gaaattattg atgaaaatgg ccaaactcta ggagtgaata ttgatccaca aacatttaaa
     3421 ctctcagggg caatgccggc aactgcaatg aaaaaattaa cagaagctga aggagctaaa
     3481 tttaacacgg caaatttacc agctgctaag tataaaattt atgaaattca cagtttatca
     3541 acttatgtcg gtgaagatgg agcaacctta acaggttcta aagcagttcc aattgaaatt
     3601 gaattaccat tgaacgatgt tgtggatgcg catgtgtatc caaaaaatac agaagcaaag
     3661 ccaaaaattg ataaagattt caaaggtaaa gcaaatccag atacaccacg tgtagataaa
     3721 gatacacctg tgaaccacca agttggagat gttgtagagt acgaaattgt tacaaaaatt
     3781 ccagcacttg ctaattatgc aacagcaaac tggagcgata gaatgactga aggtttggca
     3841 ttcaacaaag gtacagtgaa agtaactgtt gatgatgttg cacttgaagc aggtgattat
     3901 gctctaacag aagtagcaac tggttttgat ttgaaattaa cagatgctgg tttagctaaa
     3961 gtgaatgacc aaaacgctga aaaaactgtg aaaatcactt attcggcaac attgaatgac
     4021 aaagcaattg tagaagtacc agaatctaat gatgtaacat ttaactatgg taataatcca
     4081 gatcacggga atactccaaa gccgaataag ccaaatgaaa acggcgattt gacattgacc
     4141 aagacatggg ttgatgctac aggtgcacca attccggctg gagctgaagc aacgttcgat
     4201 ttggttaatg ctcagactgg taaagttgta caaactgtaa ctttgacaac agacaaaaat
     4261 acagttactg ttaacggatt ggataaaaat acagaatata aattcgttga acgtagtata
     4321 aaagggtatt cagcagatta tcaagaaatc actacagctg gagaaattgc tgtcaagaac
     4381 tggaaagacg aaaatccaaa accacttgat ccaacagagc caaaagttgt tacatatggt
     4441 aaaaagtttg tcaaagttaa tgataaagat aatcgtttag ctggggcaga atttgtaatt
     4501 gcaaatgctg ataatgctgg tcaatattta gcacgtaaag cagataaagt gagtcaagaa
     4561 gagaagcagt tggttgttac aacaaaggat gctttagata gagcagttgc tgcttataac
     4621 gctcttactg cacaacaaca aactcagcaa gaaaaagaga aagttgacaa agctcaagct
     4681 gcttataatg ctgctgtgat tgctgccaac aatgcatttg aatgggtggc agataaggac
     4741 aatgaaaatg ttgtgaaatt agtttctgat gcacaaggtc gctttgaaat tacaggcctt
     4801 cttgcaggta catattactt agaagaaaca aaacagcctg ctggttatgc attactaact
     4861 agccgtcaga aatttgaagt cactgcaact tcttattcag cgactggaca aggcattgag
     4921 tatactgctg gttcaggtaa agatgacgct acaaaagtag tcaacaaaaa aatcacgatc
     4981 ccacaaacgg gtggtattgg tacaattatc tttgctgtag caggggctgt gattatgggt
     5041 attgcagtgt acgcatatgt taaaaacaac aaagatgagg atcaacttgc ttaagtaaga
     5101 gagaaaggag ccattgatga caatgcagaa aatgcagaaa atgattagtc gtatcttctt
     5161 tgttatggct ctgtgttttt ctcttgtatg gggtgcacat gcagtccaag cgcaagaaga
     5221 tcacacgttg gtcttgcaat tggagaacta tcaggaggtg gttagtcaat tgccatctcg
     5281 tgatggtcat cggttgcagg tatggaagtt ggatgattcg tattcctatg ataatcgggt
     5341 gcaaattgtg agagacttgc attcgtggga tgagaataaa ctttcttctt tcaaaaagac
     5401 ttcgtttgag atgaccttcc ttgagaatca gattgaagta tctcatattc caaatggtct
     5461 ttactatgtt cgctctatta tccagacgga tgcggtttct tatccagctg aatttctttt
     5521 tgaaatgaca gatcagacgg tagagccttt ggtcattgta gcgaaaaaag cagatacggt
     5581 gacaacaaag gtgaagctga taaaggtgga tcaagaccac aatcgcttgg agggtgtcgg
     5641 ctttaaattg gtatcagtag caagagatgg ttctgaaaaa gaggttccct tgattggaga
     5701 ataccgttac agttcttctg gtcaagtagg gagaactctc tatactgata aaaatggaga
     5761 gattgttgtg acgaatcttc ctcttgggac ctatcgtttc aaggaggtgg agccactggc
     5821 aggctatact gttacgacga tggatacgga tgtccagttg gtagatcatc agctggtgac
     5881 gattacggtt gtcaatcaga aattaccacg tggcaatgtt gactttatga aggtggatgg
     5941 taggaccaat acctctcttc aaggggcaat gttcaaagtc atgaaagaag aaaacggaca
     6001 ctatactcct gttcttcaaa atggtaagga agtagttgtg gcatcaggga aagatggtcg
     6061 tttccgagtg gaaggtctag agtatgggac atactattta tgggagctcc aagctccaac
     6121 tggttatgtt caattaacat cgcctgtttc ctttacaatc gggaaagata ctcgtaagga
     6181 actggtaaca gtggttaaaa ataacaagcg accacggatt gatgtgccag atacagggga
     6241 agaaaccttg tatatcttga tgcttgttgc cattttgttg tttggtagtg gctattatct
     6301 tacgaaaaaa acaaataact gatattcaat gtacatcatt atgaaaaaga tagcaggctg
     6361 aagggaagac cagagtactc tgaggtgatg ttaatcagga atcatggtga tttggcatga
     6421 atcataataa cggatatgag gctgggcaga ttgtgccagc ctcattgtgg gttattgttt
     6481 gtaaaacgat aggactggtc tggtaatcat tttaggaatg gaatggacag gactgggatt
     6541 ctgatttaaa atggatggtg aatcagaaag aaatgagatt ttctcgtttc tcttagcaga
     6601 taggattgtc tgttaggaaa agcgataaaa tgatgagttt gaagttaaag gaatgctgat
     6661 aaaaaatggc aaaaacaaaa aagcaaaaac gaaacaatct cctattagga gtggtatttt
     6721 tcattggaat ggcggtaatg gcgtatccgc tggtgtctcg cttgtattat cgagtggaat
     6781 caaatcaaca aattgctgac tttgataagg aaaaagcaac gttggatgag gctgacattg
     6841 atgaacgaat gaaattggca caagccttca atgactcttt gaataatgta gtgagtggcg
     6901 atccttggtc ggaagaaatg aagaaaaaag ggcgagcaga gtatgcacgc atgttagaaa
     6961 tccatgagcg gatggggcat gtggaaatcc ctgctattga tgtagatttg ccggtttatg
     7021 ctggtactgc tgaagaggta ttgcagcaag gcgctggaca tctagaggga acttctctac
     7081 cgatcggagg caattcgacc catgcggtga ttacggcaca tacaggtttg ccaacggcta
     7141 agatgtttac ggatttgacc aaacttaaag ttggggataa gttttatgtg cacaatatca
     7201 aggaagtgat ggcctatcaa gtggatcaag taaaggtgat tgagccgacg aactttgatg
     7261 atttattgat tgtaccaggt catgattatg tgaccttgct gacctgtacg ccatacatga
     7321 tcaataccca tcgtctattg gttcgggggc atcggatacc gtacgtagca gaggttgagg
     7381 aagaatttat tgcggcaaac aaactcagtc atctctatcg ctacctgttt tatgtggcag
     7441 ttggtttgat tgtgattctt ttatggatta ttcgacgctt gcgcaagaag aaacggcaat
     7501 cagaaagagc tttgaaagca ttgaaggaag ctactaagga agtgaaggta gaggatgagt
     7561 aagagtagat attcacggaa aaagagcgtg aaaaagaaga aaaatccgtt cattcttctt
     7621 ctgatttttt tggtggggct tgccgttgcg atgtatccct tggtgtctcg ttattattat
     7681 cgtattgagt caaacgaggt tattaaagag tttgatgaga cggtttccca gatggataag
     7741 gcggaacttg aggagcgttg gcgcttggct caagccttca atgcgacctt gaaaccatct
     7801 gaaatcctcg atccttttac agatcaggaa aagaaacagg gagtttcaga atatgctaac
     7861 atgctaaaag ttcatgagcg tatcggatat gtagaaattc ctgcgattga acaggaaatc
     7921 cccatgtatg ttggcacaag tgaagacatt cttcagaaag gggcagggct gttagaaggg
     7981 gcttcgctgc ctgttggagg tgaaaatacc catacagtga tcactgctca cagaggattg
     8041 ccaacggcag aattgttcag tcaattggat aagatgaaga aaggggatat cttttatctt
     8101 cacgttttag accaggtttt ggcctatcaa gtggatcaga tagtgacggt ggagccgaat
     8161 gattttgagc ctgttctgat tcaacatgga caagattatg cgactttact gacttgtacg
     8221 ccatacatga ttaacagcca ccgtttgttg gtacgtggga aacggattcc atatacagcg
     8281 ccgattgctg agcggaatcg agcggtgaga gagcgtgggc aattctggtt gtggttatta
     8341 ctaggagcga tggcggtcat ccttctcttg ctgtatcgcg tgtatcgtaa tcgacggatt
     8401 gtcaaaggac tagaaaagca attggagggg cgtcatgtca aggactaaac tacgagtctt
     8461 attgggatac ttgttgatgt tggtagcctg tttgattcct atttattgtt ttggacagat
     8521 ggtgttgcag tctcttggac aggtgaaagg tcatgctaca tttgtgaaat ccatgacaac
     8581 tgaaatgtac caagaacaac agaaccattc tctcgcctac aatcaacgct tggcttcgca
     8641 aaatcgcatt gtagatcctt ttttggcgga gggatatgag gtcaattacc aagtgtctga
     8701 cgaccctgat gcagtctatg gttacttgtc tattccaagt ttggaaatca tggagccggt
     8761 ttatttggga gcagattatc atcatttagg gatgggcttg gctcatgtgg atggtacacc
     8821 gctgcctctg gatggtacag ggattcgctc agtgattgct gggcaccgtg cagagccaag
     8881 ccatgtcttt ttccgccatt tggatcagct aaaagttgga gatgctcttt attatgataa
     8941 tggccaggaa attgtagaat atcagatgat ggacacagag attattttac cgtcggaatg
     9001 ggaaaaatta gaatcggtta gctctaaaaa tatcatgacc ttgataacct gcgatccgat
     9061 tcctaccttt aataaacgct tattagtgaa ttttgaacga gtcgctgttt atcaaaaatc
     9121 agatccacaa acagctgcag ttgcgagggt tgcttttacg aaagaaggac aatctgtatc
     9181 gcgtgttgca acctctcaat ggttgtaccg tgggctagtg gtactggcat ttctgggaat
     9241 cctgtttgtt ttgtggaagc tagcacgttt actacgaggg aaataaaaag aaatgaaagg
     9301 aaagctaagg ctgttccttt ttc
//
LOCUS       DAVHIT010000079         9287 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVHIT010000079 DAVHIT010000000
VERSION     DAVHIT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.081"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            452..1495
                     /locus_tag="VVN16_001682"
     CDS             452..1495
                     /locus_tag="VVN16_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU5573020.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1662..3749
                     /gene="topA"
                     /locus_tag="VVN16_001683"
     CDS             1662..3749
                     /gene="topA"
                     /locus_tag="VVN16_001683"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5573021.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEVASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3855..4214
                     /locus_tag="VVN16_001684"
     CDS             3855..4214
                     /locus_tag="VVN16_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU5573022.1"
                     /translation="MRLIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4307..4939
                     /locus_tag="VVN16_001685"
     CDS             4307..4939
                     /locus_tag="VVN16_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU5573023.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPAAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQLGVTQLHGTKVVF"
     gene            4983..5639
                     /locus_tag="VVN16_001686"
     CDS             4983..5639
                     /locus_tag="VVN16_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU5573024.1"
                     /translation="MFEIFKSYQFNQEKVHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5658..6290
                     /locus_tag="VVN16_001687"
     CDS             5658..6290
                     /locus_tag="VVN16_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU5573025.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNICQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6318..6752
                     /locus_tag="VVN16_001688"
     CDS             6318..6752
                     /locus_tag="VVN16_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU5573026.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6764..7801
                     /gene="leuB"
                     /locus_tag="VVN16_001689"
     CDS             6764..7801
                     /gene="leuB"
                     /locus_tag="VVN16_001689"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU5573027.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERIAGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSV
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            7798..8067
                     /locus_tag="VVN16_001690"
     CDS             7798..8067
                     /locus_tag="VVN16_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU5573028.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8071..8430
                     /gene="leuD"
                     /locus_tag="VVN16_001691"
     CDS             8071..8430
                     /gene="leuD"
                     /locus_tag="VVN16_001691"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU5573029.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8592..9215
                     /locus_tag="VVN16_001692"
                     /pseudo
     CDS             8592..9215
                     /locus_tag="VVN16_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2871 a         1635 c         2063 g         2718 t
ORIGIN      
        1 ttagtgtaat tgagaaagga gaaatgattg tgattgatgt tggctaggtt attttcaatg
       61 attcctaccg tctcaaatct tgtcagtaag gaaaaataaa ttcttcaaaa gtagagatta
      121 caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa tggttattat
      181 aagaaatggt ctaaaataga tgatgatact tttcgaaaat ctttttatgt cagctcagct
      241 ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt tcatttacta
      301 gaaatgaaac tgatgagaga tatcagtaga catttgagtc aggatattat ggaaaatgat
      361 aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg ttaggatttt
      421 agcgactagt tagctgggaa aggaagatat tgtgacaaat aataaactgt attcgttgat
      481 agaatttaga aataaaatat atgaagaatt agaactttcc agaagtgatt tagcgatttt
      541 actatgtgcc atgcttatcg cctctatcgg attaaatatg gattcgactc ccgtgattat
      601 tggagccatg ttaatctctc ctttgatgac acctattctg ggagtggggc tctctctagc
      661 tatatttgat tttaaattgt taagaaaatc ttttaaaata ttagctattc aaattcttgc
      721 cagtttaata gcttcaacac tttattttta tctttctccc atttcgtatg ctagttcgga
      781 aattgttgct agaacctctc cgactatttg ggatgttctc attgcttttg taggagggat
      841 agcaggtatt attggtgcta ggaaaaaaga gaccaataat attgttcctg gtgttgctat
      901 tgcaaccgcc ttgatgcctc ctctttgtac agtaggttat gctattgctt ctgctaatct
      961 aaaatttatc ataggctcct cttacctatt cctcatcaat tgtagcttta ttgtcattgc
     1021 gacttatata ggtgttaggt tgatgatggt taagaaacat tattttaaag ataatgaaga
     1081 agactctaaa atgcgtagga ttttgcttct agttgctgtt ttgctgatga ttccgagttt
     1141 catctctgca acgactttag tgagagaaac gttgaaaaaa gagtccctta agaaatttat
     1201 atcagagcag tttcaggggc ataatatttt gaaaaaaacc tattctaaaa agactcatac
     1261 cctaaagcta accatttcag gaaattattt gacagaagaa gaactcgata tgatttcaag
     1321 taagagaggt gactatggtt taagtgatgt ttctgttcaa gtttcacaat tgtctgattc
     1381 agaacaactt agtaaggaag aactggtgga gtatttcttc cagtatatca aggataagga
     1441 agcaaaagaa aaggaaaaag ctaataagtt ttatacagag tctgaggagc aataatttct
     1501 tgagaatagc tggtttttct cgtgagtctt ctatgtatat caaaggaaga ctgaggtttt
     1561 aagtataaaa cttttcttct attatagtag ctaagtcttg acagggttca aaaaatggtt
     1621 tacactttat aaagtttatt actttgaaaa ggtgtgatac tgtggctacg gcaacaaaaa
     1681 agaaaaaatc aacagttaaa aaaaatctag tcatcgtgga gtcgcctgct aaggccaaga
     1741 cgattgaaaa atatctaggc agaaactaca aggttttagc cagtgtcggg catatccgtg
     1801 atttgaagaa atccagtatg tccgtcgata ttgaaaataa ttatgaaccg caatatatta
     1861 atatccgagg aaaaggccct cttatcaatg acttgaaaaa agaagctaaa aaagctaata
     1921 aagtttttct cgcgagtgac ccggaccgtg aaggagaagc gatttcttgg catttggccc
     1981 atattctcaa cttggatgaa aatgatgcca accgtgtggt cttcaatgaa atcaccaagg
     2041 atgcagtcaa aaatgctttt aaagaacctc gtaagatcga tatggacttg gtcgatgccc
     2101 aacaagctcg tcggatcttg gaccgcttgg tagggtattc gatttcgcct attttgtgga
     2161 agaaggtcaa gaagggcttg tcagcaggtc gcgttcagtc cattgccctt aaactcatca
     2221 ttgaccgtga aaatgaaatc aatgccttcc agccagaaga atactggaca gttgatgctg
     2281 tctttaaaaa gggaaccaaa caatttcatg cttccttcta tggagtagat ggtaaaaaga
     2341 tgaaactgac cagcaatgac gaagtcaagg aagtcttgtc tcgtctgacg agtaaagact
     2401 tttcagtaga tcaggtggat aagaaagagc gcaagcgcaa tgctccttta ccctatacca
     2461 cttcatctat gcagatggat gctgccaata aaatcaattt ccgtactcga aaaaccatga
     2521 tggttgccca acagctctat gaaggaatta atatcggttc tggtgttcaa ggtttgatta
     2581 cctatatgcg taccgattcg actcgtatca gtcctgtagc gcaaaatgag gtggcaagct
     2641 tcattacgga tcgttttggt agtaagtatt ctaagcacgg tagcaaggtc aaaaacgcat
     2701 caggtgctca ggatgcccat gaggctattc gtccgtcaag tgtctttaat acaccagaaa
     2761 gcatcgctaa gtatctggac aaggatcagc tcaagctata tacccttatc tggaatcgtt
     2821 ttgtggctag ccagatgaca gcggccgttt ttgataccat ggctgttaaa ttgtctcaaa
     2881 aaggggttca atttgctgcc aatggtagtc aggttaagtt tgatggttat cttgccattt
     2941 ataatgattc tgacaagaat aagatgttac cggacatggt tgttggagat gtggtcaaac
     3001 aggtcaatag caaaccagag caacatttca cccaaccgcc tgcccgttat tctgaagcaa
     3061 cactgattaa aaccttagag gaaaatgggg ttggacgtcc atcaacctac gcgccaacca
     3121 ttgaaaccat tcagaaacgt tattatgttc gcctggcagc caaacgtttt gaaccgacag
     3181 agttgggaga aattgtcaat aagctcatcg ttgaatattt cccagatatc gtaaacgtga
     3241 ccttcacagc tgaaatggaa ggtaaactgg atgatgtcga agttggaaaa gagcagtggc
     3301 gacgggtcat tgatgccttt tacaaaccat tctctaaaga agttgccaag gctgaagaag
     3361 aaatggaaaa aatccagatt aaggatgaac cagctggatt tgactgtgaa gtgtgtggca
     3421 gtccaatggt cattaaactt ggtcgttttg gtaaattcta cgcttgtagc aatttcccag
     3481 attgccgtca tacccaagca atcgtgaaag agattggtgt tgagtgtcca agctgtcatc
     3541 agggacaaat tattgagcga aaaaccaagc gtaatcgcct cttctatggt tgcaatcgct
     3601 atccagaatg tgaatttacc tcttgggaca agcctgttgg tcgtgactgt ccaaaatgtg
     3661 gcaacttcct catggagaaa aaagtccgtg gtggtggcaa gcaggttgtt tgtagcaaag
     3721 gcgactacga ggaagaaaag attaaataag aggaggggcc cgaaagtaaa tttcaggctc
     3781 cttttggtta gaacttgaca aaatacatca ttttatgcga aactagaaga agattatttt
     3841 aactaggaga agttatgcgt cttatctatc tgataattgg ttttttatca ctgaccttgg
     3901 ctattgttgg ggttgtttta cccttgttgc cgacaacgcc tttccttttg ttgtctattg
     3961 cttgtttctc cagaagttcc aagcgtttcg aagactggct ttatcatacc aagctctatc
     4021 aaacatatgt agctgatttt cgcgagacta agtctattac gcgtgaacga aagaaaaaaa
     4081 tcatcgtctc tatctacgtc ttgatgggaa tttctattta ttttgcacct cttttaccag
     4141 tcaaaatcgg tctgggtgct ttgaccatct ttatcactta ttatcttttc aaggtcattc
     4201 cagacaaaga atagttaaaa cagtagttat ttgccttgat aaaattgaaa gcatattcat
     4261 aacaatatga tataataaat ttgaagtaat attcaaggag aatcaaatga tttacgaatt
     4321 ttgtgctgaa aatgtgactt tacttgaaaa agcgatgcag gctggagctc gtcggattga
     4381 actctgtgat aatctagcag ttggtgggac aacacccagc tatggagtga ctaaggcagc
     4441 ggttgaactg gcagctaact acgatacaac catcatgacc atgattcggc cacgtggtgg
     4501 tgactttgtc tataatgacc tagaaattgc tatcatgcta gaagacattc gtttgactgc
     4561 tcaggctgga agtcaagggg ttgtatttgg agctttaact gctgataaaa agttggataa
     4621 gcctaatctg gaaaagttaa ttgctgcatc aaaaggaatg gaaattgtct ttcacatggc
     4681 ctttgatgaa ctaagtgatg aagatcaacc ggcagctatt gactggctca gtcaagccgg
     4741 tgtcactcgt atcctaactc gtgctggtgt gtctggcgac tccttagaaa aacgttttgt
     4801 tcactatcac agaattttgg agtacgctaa aggtaaaatt gaaattctac caggtggggg
     4861 gattgacctt gacaaccgtc aaacctttat cgaccagttg ggggtaacac aattgcatgg
     4921 tactaaggtt gttttttaaa aaatagaaag gaactgctag ctttgggtag cagttttcac
     4981 ttatgtttga aatttttaaa tcctatcaat ttaatcaaga aaaggttcat gattatggtt
     5041 ttatagaaaa tagcgaagtc tggacatata gttgccagat tttgcaaggt gactttgtca
     5101 tgactgtgtc catcactgct gataatgtga actttcaagt ctttgaccaa gagactggtg
     5161 acctctatcc tcacgttcat atggaaagca tgaggggaag ttttgtcgga aatgtccgtg
     5221 aggcttgtct ggagattctt taccagattc ggaaggcttg ttttgatgtg caagatttta
     5281 tctgtcatca gactaagcgt atcatgactc aagttcagga aaagtatgga aaccagttgg
     5341 agtatctgtg ggaaaaatcg cctgatacag ctgtattgcg ccatgaaggc aatcaaaagt
     5401 ggtatgccgt cttgatgaaa atctcttgga ataagctgga aaagggcaga gaaggacaag
     5461 tggaagcagt caacctcaag catgaccaag tagctaattt gctttcacaa aaggggattt
     5521 atccagcctt ccatatgagc aagcgctact ggattagtgt gtcccttgat gatactttat
     5581 cagatgaaga agtactggaa ttgatagaaa aaagttggaa cttaacctct aaaaaatgaa
     5641 atattttaat aattttcatg aactttcaat tagctaaata ttctttactg aagaaatttt
     5701 cagaaaatat aggatttacc acaccagagg aatgtggtgc catcttcaaa tacctgattg
     5761 agaatgtcaa gacggatcgt cagatcatct attcgcctca ctgtcatgat gacctcggaa
     5821 tggcagtggc aaatagcctt gctgctgtca agaatggtgc aggacgtgtt gaagggacta
     5881 tcaatggtat tagggagcga gctgaaaatg ctgctttgga agaaattgca gtggctctca
     5941 atatttgcca agattactac caagtagaaa ccagtattgt cctaaatgag accatcaata
     6001 cgtcagaaat ggtttctcgc ttctctggta ttccagttcc taaaaacaaa gccgtcgttg
     6061 gtggcaatac cttctcccac gaatctggta ttcaccaaga tggagtcctt aaaaatcctc
     6121 tcacttatga gatcatcaca cctgaattgg ttggtgttaa gattccgctt ggaaaattat
     6181 ctggtcgcca tgcttttgtt gagaaactga gagaattggc cctagatttt acagaagagg
     6241 atatcaaacc actctttgct aagttcaagg cactggccga taagaagtaa gaaatcacag
     6301 atgcagatac tcgagctttg gtagctggaa ccatggttga aaatccagaa ggcttccact
     6361 ttgatgattt acaacttcaa actcatgcag ataatgacat tgaagcgctc gttagcctag
     6421 ccaatatgga tggtgagaaa gtcgaattta atgcgacagg gcaaggttcc gttgaagcaa
     6481 tctttaacgc tatcgataag ttctttaacc aatctgttcg tttggtgtcc tacactatca
     6541 atgcggtaac agatggaatc gatgcccagg atcgggtttt ggtcactgtt gaaaacagag
     6601 atacagaaac catctttaat gcagcagggc ttgattttga tgtgttgaag gcttctgcta
     6661 ttgcctatat aaacgctaat acctttgttc aaaaagagaa tgcaggtgag atgggacgca
     6721 gtgtttctta ccacgatatg cctagtgtgt aaaggagaag gctatggcaa agaaaatagt
     6781 agctctagta ggagacggaa ttggcccaga aatcatggag gctggtttag aagttctgga
     6841 ggctctagct gaaaaaacag gttttgacta tgagattgac agacgaccgt tcggaggtgc
     6901 agatattgat gcagcaggac ctcccttacc tgatgaaacc cttaaggcaa gtagggaagc
     6961 agatgctatc ctactagtag ctatcggtag tcctcagtat gatggagtag cggttcgccc
     7021 tgaacaaggc ctgatggctc tccgtaagga actcaatctt tacgctaata ttcgtcctgt
     7081 aaaaatcttt gacagtctca agtatttgtc accactcaaa ccggaacgaa ttgctggtgt
     7141 agactttgtc gtggtgcgtg aattgacagg cgagatttac tttggagatc atattcttga
     7201 agagcgcaaa gcgcgtgata tcaacgacta tagctatgag gaagtggagc ggattattcg
     7261 caaagccttt gaaattgcaa gaaatcgcag aaaaatcctt actagtatcg ataagcaaaa
     7321 tgttctagcg acctcaaaac tctggcggaa agtagctgag gaagtcgcac aggatttctc
     7381 agatgtaacc ttggaacacc agctggtaga ctcagctgct atgcttatga ttaccaatcc
     7441 tgctaagttt gatgttattg taacggagaa tctttttgga gatattttat ctgatgaatc
     7501 aagcgtctta tctggtacac ttggggttat gccatcagcc agtcattctg aaaatggacc
     7561 aagtctctat gaacctattc acggttcagt acctgatatt gcaggtcaag gaattgccaa
     7621 tcctatttcc atgattttat cagttgtcat gatgttgaga gatagtttcg gacgttatga
     7681 ggatacagag cgtatcaaac gtgctgttga gacaagtctg gcggcaggaa ttttaacgag
     7741 agatatagga ggtcaggctt caacaaagga aatgatggaa gctattattg caaggttatg
     7801 aagttagacg aaaaaattac tctagtcctt ttgatttgga atgtcatcat tttcttgatt
     7861 tatggcattg acaaattcaa ggcaagaaga agagcttggc gcatcccaga gaaaatctta
     7921 ctcattttag cccttacttg tggtggtttt ggggcctggt tagctggaat cacttttcac
     7981 cataagactc gaaaatggta ctttaaaaca gtttggtttc ttgggatggt gaccacacta
     8041 gtagccttat attttatttg gaggtaatgg atggcagggt cttcgaggga acacgctgct
     8101 tgggctctag cggactatgg ttttaaggtc gtgattgcag gatctttcgg tgacattcat
     8161 tacaataatg aactcaataa tggcatgttg ccaatcgttc agcctagaga ggttagagag
     8221 aaactagccc agctaaaacc aaccgaccag gtaactgtgg acttggaaca acaaaaaatc
     8281 atctcaccag ttgaagaatt caccttcgag atagatagca agtggaaaca taaactccta
     8341 aatagtttgg atgatatcgg tattaccttg cagtatgaag agttgattgc tgcttatgaa
     8401 aaacaacgac cagcctactg gcaggattag aaaaaataga aaaggaaata tagtaaactg
     8461 aaataagatg taaacaaatg aattggagct taacatccat ttccagcaat tttttagaaa
     8521 ctacagtgga ctattctgga ttcaacacat tataaaatta tgacaaaaca cattcacaag
     8581 aaggctacga cattttaaaa ggtgagggcg gatgtatcgt ttgccctact aaagttggtt
     8641 acattatcat gaccagcaac aaggcaggac ttgagcgtaa gttcgcagcc aaagaacgta
     8701 agcgtaacaa accaggtgtt gttctctgcg gtagcatgga tgaacttcgc gctttagcgc
     8761 aactcaaccc agaaattgaa gcattctact aaaaacattg gatgaagata ttcttcttgg
     8821 ttgtatcctt ccttggaaac cagaagcctt tgaaaaactc aaagcatacg gggatggccg
     8881 tgaagaactt atgactgatg tacgtggtac tagctgtttt gttatcaagt ttggaaaagc
     8941 aggtgaacaa ttggctgcca agctttggga agaaggtaaa atggtctacg cctcatatgc
     9001 ttcaatgaca aaacgattga aactcgctat gagagcaagg tgtaatggtg tctatggtcg
     9061 ataaggacgg caaactcatc ccagaacaag gaggagcacg ttcaacttca ccagcaccag
     9121 ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca gatactttta
     9181 actcatggga ctaccgtcag gttgagtatt attaagataa aaaaagaagt ctagtgttat
     9241 gagatattaa agcttctaac actggacttt ttgtttagaa tttcttt
//
LOCUS       DAVHIT010000080         9286 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVHIT010000080 DAVHIT010000000
VERSION     DAVHIT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.082"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(267..1463)
                     /gene="tuf"
                     /locus_tag="VVN16_001693"
     CDS             complement(267..1463)
                     /gene="tuf"
                     /locus_tag="VVN16_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5573030.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYAAIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2374..3243)
                     /locus_tag="VVN16_001694"
     CDS             complement(2374..3243)
                     /locus_tag="VVN16_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU5573031.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3466..3987)
                     /locus_tag="VVN16_001695"
     CDS             complement(3466..3987)
                     /locus_tag="VVN16_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5573032.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDKK"
     gene            complement(3959..5050)
                     /locus_tag="VVN16_001696"
     CDS             complement(3959..5050)
                     /locus_tag="VVN16_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU5573033.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTIITVRMLRKSGSQNSNLY"
     gene            complement(5161..5415)
                     /locus_tag="VVN16_001697"
     CDS             complement(5161..5415)
                     /locus_tag="VVN16_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573034.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5755..6288)
                     /locus_tag="VVN16_001698"
     CDS             complement(5755..6288)
                     /locus_tag="VVN16_001698"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU5573035.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLERFFKTR"
     gene            complement(6275..6490)
                     /locus_tag="VVN16_001699"
     CDS             complement(6275..6490)
                     /locus_tag="VVN16_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5573036.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(6457..6600)
                     /locus_tag="VVN16_001700"
     CDS             complement(6457..6600)
                     /locus_tag="VVN16_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU5573037.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            6834..8552
                     /locus_tag="VVN16_001701"
     CDS             6834..8552
                     /locus_tag="VVN16_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU5573038.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            8663..9010
                     /locus_tag="VVN16_001702"
     CDS             8663..9010
                     /locus_tag="VVN16_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5573039.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
BASE COUNT         2698 a         1915 c         1714 g         2959 t
ORIGIN      
        1 tacaacctaa aaacagtgtt ttgagcagtc tgcagctaac tttctataac aaaatgaaat
       61 aagaacaaga caaatcaatc aagataatca aatcgatttc taacaatgtt ttagaagtag
      121 atgtgtacta ttctagtttc aatatattat agtttgctct ttgattttca ttgagtatta
      181 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct gacttaaata
      241 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
      301 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
      361 atcaactcaa cgtcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
      421 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
      481 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
      541 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
      601 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
      661 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaaca
      721 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
      781 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
      841 cgttctggtt ctgggatata ctcatcaact gtgttcatca attccataac gatgtcttcg
      901 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
      961 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
     1021 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
     1081 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
     1141 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
     1201 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
     1261 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgatagc cgcatagtct
     1321 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
     1381 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
     1441 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttttatagat
     1501 agacagtagg caatacagtc taactttcct tactatttta tcaaatttca gctaaattgc
     1561 aagtgtttta caacgttttt gtgaattatt cttggtaaaa atttaactaa ttaatagaaa
     1621 gtactaaata gacttatcct tcaaggagtt ttcagcatta tccatccact cacttgctga
     1681 aaatgtaaac agtctctggt ttatatagta aaatgaaaca agaatagggc aaatcgatca
     1741 ggacagtcaa atcgatttct aacaatgttt tagaagtaga tgtgtactat tctagtttca
     1801 atctactata caacctcaaa actgtgtttt gaacaacctg cggctagctt cttagtttgc
     1861 cctttgattt tcattgagta taaaattgca gataaaacga taacaatcat aggactcagc
     1921 agagtcaatg taggtcgaag aatagccccc ctaatcgctt attttatagc aaaatgaaat
     1981 agaaactgaa cattttgttg aggaaggtaa atcaattcct cacaatgttt gagaaagaag
     2041 gggggactat tccaacttca acccactata aagcagactt acaagatagg gaacccatct
     2101 ccgataatat atagtagaat gaaaactgca caatttgatt ggagaattca aaccaatttc
     2161 taacaatgtt ttagaaacaa gggtctatta tttcaactgc aacctactat agcccattga
     2221 agctggaata gcccactgag acttctaaac cactgctgga gaggagtttg tttagagttt
     2281 ctttcatttc cctaaaaaat caaaaagaga gaaccagtct gatcctctct tacttataga
     2341 tggtgctcac tcaaatgtta aaggagctat ttcttagaga taaaggaatt tgaatacagc
     2401 tactgccgca attgctgctg cgataggtgc tactactggt acccaagaat accaccattt
     2461 tgaatcgcct ttatgctcac caagaactga ttttggaagg aaagcatgaa ggagacgtgg
     2521 tcccaagtca cgggctgggt tcaaggcagg tcctgtaggt cctccaagtg atgttaccaa
     2581 agccataacg aggaatccaa gtgccaagtg agccacagaa agtcctgaag cagtgtgtgg
     2641 agccacctgt gctttaatag ccaagtcaga aaaatcaact gtttgtcctg cttctattgc
     2701 cttttgtttc atgaattgaa gcacttcagc accaaagaag tttttagtca aaccaagagc
     2761 tgcaaagaac aaaacaaatg aaccaacaaa ctcattaatc aaaccattga cagttgctgc
     2821 atagcgactt tcttttgtac catggtcaat acttgaaata gttgagaaag ttcccaagat
     2881 gttatttggt ttttcagttt tcaagtagaa tggacggtat gttgccacaa ctaaggcttg
     2941 gccaaagata gcccccaaga cttgcgcgat aatgtaaggt accacttgtg cccaagggaa
     3001 aagaccgcta actgcaagcc ctagagtgaa agcagggttg atgtgattcc cagatacgtt
     3061 accaaacatc aaggctggga tcataacccc cataccataa ccaacagcga tgacgatcca
     3121 gccactttgg tgacctttcg tacctttaag ttcaacgttg gcaactgcac cattcccaag
     3181 aatgatcaaa atggcagttc ccaaaaattc agtggcatac ttcagtgccc atgtgaaatc
     3241 catttgctag attctcctta aaatttttta gatattcctt attttatcaa tttttaccct
     3301 atttagcaac tgatttctgg aaatattttt aaaaaaatag gattcatagt ttcccagttc
     3361 aacgtacttg taagcgtttt tattcttcac gaaagacctc attctcaaaa ccaggaaaaa
     3421 gcccacatct gtgatctttt ccttgttcct agattctaaa atctattatt ttttatcttc
     3481 ttttttcttg aagaatccta atcctagacc agctaacaag gtcatgatac cagcactagc
     3541 caaggtagca ttagcttctg tacctgtgtt tggcaattcc tctctcttac ctgtctcata
     3601 agtcggaact tctgggtctg gattcactgg agtttcagtt tttggagtac ctggttctgg
     3661 agttggttta tctggtgttg ataaacggtc ataccttacc gttatttctt tatcactaga
     3721 gtctgacgta acttcttgtg attcaactgt tggaatatct ggatctttgt acttgtcaat
     3781 cttaccagat ataacctcgt cccagtttcc tgttgtccat tcaccgtagg ttacaactcc
     3841 cgtgaccttg ttctcagttt ttgtacggct taaggttaca ggttgaacaa catcttcttt
     3901 tacattttgg ttcgtaactt tatcaacgta atgaatgata cgcgttatag tcttcgtctc
     3961 agtagaggtt gctgttttgg gaaccacttt tcctcaacat tctcacggta ataatagtca
     4021 actgttgcac cgtcttctgg tacgcatttg caggagttgc taccaaggtg tatgttgttt
     4081 tccttgtgat aactcggtct tctttgtcct cagttgttgt tttcccttca atagtttttg
     4141 attctgtggt atactcagaa cctatcgcta aatcagcttt tataacagac tctgccaact
     4201 tctcttggct accttcttta tagtaattgg ctgttactgt agcagtggtt ggcgcttcgc
     4261 tttctctata aactaaggtc actgttctac cttcgcttac aatattccca gttaaacttg
     4321 cagaatttgt atctgcttct ttaaaagtat aatattttcc gtcagtagta gtcatgctac
     4381 tgagtttttt atctgtgaca ttgtagctgg taccaatcag ttgtttttta ttggtaatgt
     4441 aggttccgtc actttctttt tctccaattc cagtatcatt tttcaatgat agcaaacgcc
     4501 cttgttcatc aacatagcga actttcacat tttctgagat tagttctgcc aattctgagg
     4561 tttttttctt tttcttgatt tcttcggtta ttttcccttt ctcttcttcg ggaatattta
     4621 gttttggaat gattttttca acaacggttc gtgatggttc cacagtatct tggatgactg
     4681 aaaagtcagc tagaattggg agattatact gaacacggtg actttgagtg tttactccta
     4741 ctctttcatt attctctgaa aatacttgta cggtataaga aacaacatct tttcctaata
     4801 gaacatcccc agtagagaaa tagccgcctt ttcctagttt gctatctcca gagcccactt
     4861 ctttcctaat cttatcagat agttttttac cagtcagtac attcgttcgc acaatccctt
     4921 tgtctacccc tacaaagtgg gagaactttt tgaactcttc agaaccagat ctagcccaac
     4981 cattattaag ggcatttgct tttgtatttg tattctctct caaaagtttg gcgattagaa
     5041 ttatattcat cggcacttag agttgctgct atatctgact cttgaatacc aacttcctta
     5101 ctaccatttc tagcggcagt atatgtgtat ttatttgttt ctacttctag ctgattatcc
     5161 ttaacatgat aggtaatttt ctcctggtat aagagtcttc agtagtttat atgctctgtc
     5221 atcatcttta gctttagaag cagctgagtt tagccaagtt ggggtattag gagtatcttt
     5281 atcagcctgt ttcaagacat tattcaagtc atttgctagc ctattggttg tcctcactgt
     5341 gaaagttttc ttccagtctg aaccactttt attagactta gatatgctca tgaaatcctg
     5401 gtcaacattc aacacaatat cgccttgttt tttagcatct tccgttgctt ctgcttcaaa
     5461 ttgatcatgg agtttttgtt gctctggtgt atagtttttt tatttacacg atttaatgta
     5521 ctatttttta ttttagtatt aagaactcca ttaatacgat ttttaaactc tgcttcaaaa
     5581 aatcccttaa tttgttggtg cgcaagttca acattcaaac ctttcttata caagtcttca
     5641 ttctgaacga tttcggctac acggtgaatg gtttgatcgt ataccgtctt ctcttaacca
     5701 gcagtatcct tagttttaat gactactcga taagttcctt tagaaccgtt atagtcatct
     5761 agttttaaaa aagcgttcca aacattcaaa aatagttgga acttccctta ataaatccgt
     5821 cactttcttt ttcaagatag cccaagcttg ctcaatagga ttcaaatccg gtgaataagg
     5881 tggtagaggt aagaaaaagt gtttatcttg gatgcagagt tcatccaaaa tgttcttggg
     5941 atgaaaacta gcattgccca tgacaataac atgaggtgtc ttcaaagcag gtaggagttg
     6001 cgttttgaac cactccacaa agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc
     6061 tataaactct ccgtctactt gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct
     6121 aatcttttca tagactttct cccctctagg agcccctgca taaggacgat agaggtagcg
     6181 gtcgattcct gtttcgtcaa tatatacgat tggtaaatct tttaggttat ccaaaatatc
     6241 aagaaactca gcgactttct ctggctcttg ttccttaaag ctcgtcatct ttttttaaag
     6301 tgaccttaat ctgctttaaa gctgcccata ctgaaggaac agcacaatca aaatgtgccg
     6361 caatttcccg taaaaaagca tctggatgag cctctacaaa ggctttcaat tcctctaaag
     6421 gaatctttcg gtttttgacg actcgctttt tcctctctat gtgtccttgt tcacatcttt
     6481 cttttcccac gtgaagagag ttctgacgcc aacaccaaaa aacttggctg cctcgacatg
     6541 gctgtgcccc tctttgatgt aatctaatgc tcgctgttta aaatcagtac tatatgccat
     6601 agctttatta taatatgctt gtttaaaact aagcaactat atcaatgttt tgttattaga
     6661 agacttactt tttttactga aatcgaattt ttcctaacct caacatcata taaccccaaa
     6721 attcaatctg acctggtttg aagagatgtt cgaagcagat aaggttttac taaaactaaa
     6781 aaaaatgata taatcaaact gattaaatgt tttaaaaata aaggagaatt tgaatgtctt
     6841 accaagaaaa ttaccagaaa tgggttgatt ttgtggagct tcctgactac cttcgtcaag
     6901 atttggaaaa tatggacgaa aaaactaagg aagatgcctt ctatacaaat cttgaatttg
     6961 gtactgcagg tatgcgtggc ttggttggtg ctggtacaaa ccgtatcaac atctacgttg
     7021 ttcgccaagc tactgaagga ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag
     7081 aacgcggtgt agcaattgcc tacgatagcc gtcacttctc acctgagttt gcctttgaat
     7141 ctgcggcagt tcttgctaaa cacggcatca aatcttacgt atttgaaagc cttcgtccga
     7201 ctccagaact atcatttgca gttcgtcatc tcaactgttt cgcaggtatc atggtcacag
     7261 ccagccacaa ccctgctcca tttaacggtt acaaggttta cggtgaagac ggtggacaaa
     7321 tgcctccaca cgatgcggac gctttgacta cttatatccg tgcaatcgaa aacccatttg
     7381 cagttgaagt tgctgatgtg gaaactgaaa aagcttctgg cttgattgaa gttatcggcg
     7441 aagctgttga cgtagaatac cttaaagagg ttaaggacgt aaacatcaac ccagccttga
     7501 ttgaagaatt tggtaaagac atgaagattg tctacacacc acttcatggt actggtgaaa
     7561 tgttggctcg tcgtgctctt gcccaagcag gatttgactc tgttcaagtt gttgaagcgc
     7621 aagcaactgc tgacccagac ttctcaactg taacatctcc aaacccagaa agccaagcag
     7681 cctttgccct tgctgaagaa cttggtcgtc aagttggtgc agatgttctt gtcgcaactg
     7741 acccagacgc tgaccgtgtt ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc
     7801 tttcaggtaa ccaaatcggt gctatcatgg ctaaatacat cttggaagct cacaaaaacg
     7861 ctggaactct tcctgaaaat gccgctctct gcaaatctat cgtttcaact gacttggtaa
     7921 cgaagattgc tgaaagctac ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta
     7981 tcgctgaaaa aatccaagaa ttcgaagaaa aacacaacca cacttacatg atgggatttg
     8041 aagaaagctt cggttacttg attaaaccat ttgtacgtga taaagatgcc atccaagccg
     8101 ttcttgtcgt tgctgaactt gctgcctact accgttctcg tggtttgaca cttgctgacg
     8161 gtatcgaaga aatctataaa gaatatggct actacgcaga aaaaacaatc tctgttactc
     8221 tttcaggtgt cgatggtgct gaacaaatca aagcgattat ggctaaattc cgcaacaatg
     8281 ctccaacaga atggaacgca acagctatca ctgtcgtaga agacttcaag gcacaaactg
     8341 ctactgttgc tgacggtact gttacaaact tgacaactcc tccaagtgat gtgttgaaat
     8401 acacacttgc tgacggttca tggattgccg ttcgcccttc aggtacagaa ccaaaaatca
     8461 agttctacat tgcagttgta ggggaaacca acgaagaatc acaagctaag attactaaca
     8521 tcgaagcaga aatcaatgca tttgtaaaat aaagacctat aaaagatgag accaaccaac
     8581 ctcatctttt ttcgtatcaa atctaagcaa ttttagaaac tttatggcat actagactta
     8641 tcaatgaaag cgaggtaatc tcatggaaca atttttagat aacatcaaag accttgaagt
     8701 cactacagtt gtgcgtgcgc aagaagctct tgataaaaaa gaaactgcaa ccttctttat
     8761 cggtcgcaaa acttgccctt actgccgtaa atttgcaggt acattgtcag gtgtcgtagc
     8821 tgaaaccaaa gctcacattt acttcatcaa tagtgaagaa ccaagccaac tcaatgattt
     8881 gcaagcattc cgctcacgtt atggaatccc aactgtacca ggttttattc atattacaga
     8941 tggacaaatc aacgtccgtt gcgactcttc aatgtcagca caagaaatca aagatttcgc
     9001 aggattgtaa aaaatcagct aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc
     9061 aaaaaccttg atattatgcg ttttattgtt cctaaaacag ttacatcagc atttcattac
     9121 agaacatttt aattttggaa gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg
     9181 gctattccat attcaattat ccagattcaa tcgctattat cgttttccta gattacaaaa
     9241 aaaactacgt tttgctaatc aaaacgtagt ctcagccact atttta
//
LOCUS       DAVHIT010000081         9264 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVHIT010000081 DAVHIT010000000
VERSION     DAVHIT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.083"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            10..1011
                     /locus_tag="VVN16_001703"
     CDS             10..1011
                     /locus_tag="VVN16_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5573040.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGKSHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1516..2694
                     /gene="trpB"
                     /locus_tag="VVN16_001704"
     CDS             1516..2694
                     /gene="trpB"
                     /locus_tag="VVN16_001704"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5573041.1"
                     /translation="MTTKGYFGQFGGSFVPEPIQALLDELEVTFDKYKDDPEFLAEFR
                     HYLKDYSGRETPLYFAESLTEHLGGAKIYLKREDLNHLGSHKLNNVLGQILLAKRMGK
                     KRVIAETGAGQHGVATAAAAAKFGMACDVYMGAEDVERQRLNVFRMEMMGATVHAVET
                     GTRTLKDAVDAAFGAWMNDLEAFYVLGSAVGPHPYPTIVHEFQKVISEESRRQILEKE
                     GRLPDYVIACVGGGSNAIGAFSQYVADEEVKLVGVEAAGHGLDTDKHAATMTKGSIGI
                     VDGMKTYAVFKEDGELAPVYSISAGLDYPGVGPEHAYFKDSGRVEYVAATDEEAVQAL
                     LLLSKTEGIIPAIESSHAIAEAVKRAPKLSKDEIIIINVSGRGDKDVATIADYLEAKK
                     "
     misc_binding    2685..2916
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2988..4349
                     /gene="trpE"
                     /locus_tag="VVN16_001705"
     CDS             2988..4349
                     /gene="trpE"
                     /locus_tag="VVN16_001705"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU5573042.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGILYQNGQVIDRDPLDFLYEVTHKRQHHSDLPFGGGAIGFVGYNMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEDLEEALNQV
                     LEELRIPAPNEFEDLDLSSLDFKPHIAPQKFEEMVETARDLIRNGDMFQCLLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDFAIAIRTMILKNQTAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            4346..4912
                     /locus_tag="VVN16_001706"
     CDS             4346..4912
                     /locus_tag="VVN16_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5573043.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            4923..5927
                     /gene="trpD"
                     /locus_tag="VVN16_001707"
     CDS             4923..5927
                     /gene="trpD"
                     /locus_tag="VVN16_001707"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5573044.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5924..6691
                     /gene="trpC"
                     /locus_tag="VVN16_001708"
     CDS             5924..6691
                     /gene="trpC"
                     /locus_tag="VVN16_001708"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU5573045.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGIALMQAENVVQRIKELQIDKG"
     gene            6678..7277
                     /locus_tag="VVN16_001709"
     CDS             6678..7277
                     /locus_tag="VVN16_001709"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU5573046.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            7255..8478
                     /gene="trpB"
                     /locus_tag="VVN16_001710"
     CDS             7255..8478
                     /gene="trpB"
                     /locus_tag="VVN16_001710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5573047.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            8471..9247
                     /locus_tag="VVN16_001711"
     CDS             8471..9247
                     /locus_tag="VVN16_001711"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU5573048.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
BASE COUNT         2730 a         1744 c         2246 g         2544 t
ORIGIN      
        1 aggactgaaa tgaaaaatat aaatgggaaa aaggtaacta tttatgatat tgcacgttta
       61 tcaggttttt ctccaaaaac agtttcacga gttattaatg gtggagtaaa tgttaaagaa
      121 gaaacttatc aagcgattca aaaagttata gaggaattgt cgtatattcc aaatgcgtat
      181 gcaaaaaatt taactaaaaa agaagctata aatatattga tttctgtaaa aaaaatcgac
      241 tcttttcctt taatttggtt tcatacgctg ttagataaag tgttacggac gtgtaaagaa
      301 tttggtgtta atgctatagt tgagtacttt ggagaggagg atacaattag taattcaatt
      361 atatcaagca caggtagcct agtagatggt gttattgttt tttatgaaag tgtagatgat
      421 atcagaattc agtatttaaa gaagaaccat atgccttttc ttgttttcgg tgaatctcaa
      481 acgtctggag tagtctatgt atctaataat aattttcaag ctacttatga tatgatgaaa
      541 gctgtaactg aagaaaaatt taaaaatatg tggttgctta tgggaggaaa atctcatgtt
      601 aataaggatc gtgaaagagg tgtgcgttct tttctgaatg ataaaaatta ctttatggat
      661 ttaaaagtca tttatggctt atctacaatt gattcagttt attcttatgc aatgcagcac
      721 ttgactaccc aaaattatcc ggacattatt tttgtttcag gagatgaaaa ggttcagggc
      781 ttgattcgtg cgtgctatga gaaaggaatt cttattccag atgatatttc tatcattgga
      841 tttgataata ttcctatttc tcagtattat actccagctt tgtctacaat tgctcccaat
      901 tatgttaaat tggctaaaga aatgatagaa ggtgttttag caattattaa aggggaaagt
      961 gtcacatctg ttgaagtttc tcctaaattt gttaggagac agagtttcta gtattgttta
     1021 attcttgaaa atcctagtgg aaaagatgga gtgttctaaa ataagttctg tttagcctcc
     1081 attcagtttc tattgagttg gatccaattt ctaacaagtg ggacatgttc atgttcgatc
     1141 taaggaagat atggtcacat tcgaatgata tcaaaaaaat cgtaaaaaag tcttgacaaa
     1201 gaaaaaacaa agtattaaaa taagttcaac aaatcagaaa agtaactatt tttgatcttc
     1261 agggagcctg tggtgattgt gaataggtgg ttggaagtag tgaaagtggg ctgattttga
     1321 aaatgaattt gaaacaatga aaattcggtg cgcacacctt acagtgcagc ttgttgttaa
     1381 acaaggcaga gatgtaaagg gatagtccct ttataattga ggtggcaccg cgttaccaac
     1441 gccctcacgc agagtaattt tctgtgtgtg ggcttttttc atatcttgaa atcaatactg
     1501 aaagaggaaa attttatgac aactaaaggt tattttggac aatttggtgg tagttttgta
     1561 ccggagccga ttcaggcttt gttggatgag ttggaagtga catttgataa gtacaaggat
     1621 gatccagagt ttttggcaga atttcgtcat tacttaaaag actattctgg tcgcgaaaca
     1681 ccgctctatt ttgcagaaag tttgacagag cacctaggtg gagctaagat ttatctcaag
     1741 cgtgaagacc ttaaccacct tggttctcac aagctcaata acgttttagg acaaattctt
     1801 ttggctaaac gcatgggcaa aaaacgagtg attgcggaaa caggagctgg tcagcacggt
     1861 gttgcgacag cagcggctgc agccaagttt ggtatggcct gtgatgtcta catgggagca
     1921 gaggatgtgg aacgccaacg tctcaatgtt ttccgtatgg agatgatggg agcaactgtt
     1981 catgcagttg aaacagggac tcgtaccctc aaagatgctg ttgatgcagc ctttggagca
     2041 tggatgaatg atttggaagc cttctacgtt ttgggatctg ctgtgggacc tcacccatat
     2101 ccaaccattg ttcatgagtt ccaaaaggtc atcagtgaag aatctcgccg tcaaatctta
     2161 gaaaaagaag gtcgtttacc agactacgtt attgcctgtg taggtggtgg ttccaatgct
     2221 ataggtgctt tttcacagta tgtggctgat gaagaagtca aattggttgg ggtcgaagct
     2281 gctgggcacg gacttgatac agacaagcac gcagctacta tgacaaaagg tagtatcgga
     2341 attgtcgacg gcatgaagac ttatgcagtc tttaaggaag atggagagct ggctccagtt
     2401 tactctatct cagctggatt ggactatcca ggggttggcc cagaacacgc ctacttcaaa
     2461 gactcaggtc gagtggaata tgtggctgcg acagatgaag aagctgttca agccctgctc
     2521 cttcttagca agactgaagg gattatccca gcgattgaaa gctcacatgc tatcgcagaa
     2581 gcagttaaac gtgcaccgaa actaagtaaa gacgagatta tcatcatcaa tgtctctggt
     2641 cgtggagaca aggacgtagc tacgattgca gactacctag aagctaaaaa ataatagatg
     2701 gaaaaattac agtcctttct ctcacagaga gcttgtggtt gctggaaaca agcagagaaa
     2761 gctgtaaggt tgggtccatc tgacacgaga gaagaaaaca taattctcaa gggagtgccc
     2821 tttatcgcac agcctgttac aggagcttcc tgagacagga atgagagata ggagaaatcc
     2881 tataattgag gtggcaccgc gaatttcgtc ctcacgcaag ttattttgcg tggggatttt
     2941 tcatacagtc gccacgaact agaattagaa ctgaaaggga aattacaatg gaacgaatca
     3001 ttcatggaga tgtcttatca ccaatcttgg cttatatgcg cctaaagggg caacacaagg
     3061 ttatcttaga gagtattccg agagacaagg aaacagctcg tttttctatc ttagcctata
     3121 atcctgtttt tgagattaag tttgaaaatg gaattcttta tcaaaatggt caagtgattg
     3181 atcgggatcc cttggatttc ctttatgaag tgactcataa gagacagcac cattcagacc
     3241 tcccttttgg tggaggagcc attggctttg tgggttacaa tatgatttca ctttatgaag
     3301 aaattggtca aattcctgaa gatacaattg gaacgccaga catgcatttc ttcgtctatg
     3361 agagttacat ggtctttgac cacaagaagg aaaaaatcca tgtcatcgag gatgctcttt
     3421 atagtgagcg tagccaagaa gacttggaag aagccttgaa ccaagtgctt gaggaattac
     3481 gcattcctgc tccaaatgaa tttgaagact tggatctatc ttcactggac ttcaaaccgc
     3541 atatcgctcc tcagaagttc gaggaaatgg tggaaacagc tcgtgacttg attcgtaatg
     3601 gagatatgtt ccaatgttta ctcagtcagc gcttctcagc agaagttact ggaaatccat
     3661 ttgacttcta cagaaatctc cgcgtgacta atccttctaa ttacctttat ttctatgatt
     3721 ttggggatta tcaaatcatc ggagctagtc cagaaagttt ggtttctgtc aaaaatggca
     3781 tcgtgacaac caatccgatt gcaggtacgc gaccaagagg agctacagat gaagaagaca
     3841 aggctttggc gacagacctg ctttcggatg agaaggaaac agcagagcat cggatgttag
     3901 tagacttggg acgaaatgat attggccgca tctctgaaac ggccagtgtc caagtcacta
     3961 agtatatgga agtggagctt ttccgttatg tcatgcattt gaccagtgtg gtcaaggggc
     4021 gtttgcttcc agaactcact gccatggatg ccttgaaagc aacacttcct gctggaaccg
     4081 tttctggagc accaaagatt cgagctatga gacgcatcta tgaactggaa acggaaaaac
     4141 gtggcgtata cgcaggagca atcggctact tgtctgcgac gggtgatatg gattttgcta
     4201 ttgccatccg aactatgatt ctcaaaaatc aaacagccta tgtgcaggct ggggcaggga
     4261 ttgtctacga ttctatcgcc caaaacgaat accaagaaac cattaacaaa gcaaaatcta
     4321 tgactagaat tggagaatta agaccatgat tttattgatt gacaactatg attcttttac
     4381 ttataacttg gcccaataca ttgggaattt tgcagaagtt caggttttga gaaatgatga
     4441 ttccaagctg tatgaagaag ctgaaaaagc agatggtctg gtcttttctc ctggtcctgg
     4501 ttggccagtt gatgctggaa agatggaaga catgattcgt gattttgcag gcaagaagcc
     4561 gattcttggg atttgtttgg gccaccaagc cattgcagaa gtctttggtg gtaagctagg
     4621 tttggctcca aaagtcatgc atgggaaaca gagcaatatc aactttgaag cgccatctgt
     4681 tttgtatcaa ggtattgagg atggccgtgc ggtcatgcgt tatcacagta ttttgattga
     4741 ggaaatgcca gaagactttg aagtgacagc tcgttcgact gatgaccaag ccatcatggg
     4801 gattcaacat aaaaacctac cgatttatgg cttccagtac catccagaga gcattggaac
     4861 gccagacggc ttgtcttcta ttcggaattt tatcgaggag gttgtaaagt gaggaaacta
     4921 ggatgaaaga gattattgaa aaactagcaa aatttgaaaa tttatcaggt gtggaaatga
     4981 cggatgtcat tgagcgtatc gtaactgggc gtgtaactga agcgcagatt gcttctctcc
     5041 tcttagctct taagatgaag ggggaaacac ctgaagaacg cacagccatt gcccaagtca
     5101 tgagaggaca tgcccagcat attccaactg agattcatga tgccatggac aactgtggta
     5161 caggtgggga caagtctttc agttttaata tttccacaac tgcagccttt gtcttggctg
     5221 gtggcggtat tcacatggcc aaacacggta accgctcgat atcttctaaa tctggttccg
     5281 cagatgtcct cgaagccttg ggaatcaatc ttgacctcaa accagctgaa ctaggtaagg
     5341 tctttgataa aactggaatc gtctttctct tcgctaaaaa tatgcaccca gctatgaaat
     5401 acatcatgcc agctcgtttg gaattgggaa ttccaacgat tatgaacttg actggtccac
     5461 tgattcatcc aatggcttta gaaacacagc ttcttggaat tagtcgtcca gaactcctag
     5521 aaagtacagc tcaggttttg aaaaatatgg gtcgcaaacg tgccatcgtg gttgctggac
     5581 cagaagggtt ggatgaagct ggcttgaacg gaacaaccaa gattgcactt cttgaaaatg
     5641 gcgaaatcag cttgtcaagc tttactccag aggatttggg aatggaaggc tatgctatgg
     5701 aagatattcg tggtgggaat gctcaggaaa atgcagaaat tttgcttagc gttctgaaaa
     5761 acgaagcaag tccattcttg gaaacgacag tcttgaatgc tggtcttggt ttctatgcta
     5821 atggtaagat tgatagcatc aaggaaggag ttgccttggc tcgtcaagtg attgctagag
     5881 gcaaggccct tgaaaaactc agactgttac aggagtacca aaaatgagtc aggaattttt
     5941 agcacgaatc ttagagcaga aggcgcgtga ggtggagcag atgaagctgg agcaaatcca
     6001 gcctctgcgc cagacctatc gcttggcaga atttttgaag aatcatcagg accgcttgca
     6061 ggtaatcgct gaggtcaaga aagctagccc tagtttggga gatatcaatc tcgatgtgga
     6121 tattgtgcaa caggcccaga cttatgaaga aaacggagca gtgatgattt cggtgttgac
     6181 agatgaggtt ttctttaaag ggcatttgga ttatctacgg gaaatttcca gtcaggtaga
     6241 gattccgacg ctcaacaaag actttatcat cgatgaaaag caaatcatcc gcgctcgcaa
     6301 tgcaggtgcg acagttatct tgctcattgt ggcagccttg tccgaagaac gcctcaagga
     6361 actgtatgac tacgcgacag agcttggtct ggaagtctta gtggagactc acaatctagc
     6421 tgaactagag gtagcccaca gacttggtgc tgagattatc ggggtcaaca accgcaactt
     6481 gactaccttt gaggtcgact tgcagaccag tgtagattta gccccttact ttgaggaagg
     6541 tcgctattac atttctgaat ctgccatttt cacagggcag gatgcggaac gactagcccc
     6601 atactttaat ggaattttgg tagggatagc tcttatgcag gcagaaaatg tggtccagag
     6661 aatcaaggag ttgcagattg acaaaggtta aaatttgtgg actatcgacc aaagaagcgg
     6721 tggaaacagc cgtttcagca ggagccgact atatcggttt tgtctttgca cctagtaaaa
     6781 gacaggtgac tttagaagag gcagctgagt tggcaaagct tattcctgca gatgtgaaaa
     6841 aggttggagt atttgtttca ccaagtcggg tagaactgct ggaagcgatt gacaaagttg
     6901 gcttggactt ggttcaagtt cacggtcagg tagcagatga tttatttgag aatttgcctt
     6961 gtgccagtat tcaggctgtg caggtagatg gaaatgggca tgtccctaat tctcaggcag
     7021 attatctact ctttgatgcc cctgtggcag gaagtggcca gccctttgat tggggtcaac
     7081 tggatacgac tggactagca cagcccttct ttatcgcagg tggccttaat gaagataatg
     7141 tagtaaaagc aattcaacac tttactccct atgcagtaga tgtatcaagc ggagtggaga
     7201 cagatggaca aaaagatcat gaaaagatta gaagatttat agagagggta aagaatggca
     7261 tatcaagaac caaataaaga tggattttac ggaaaatttg gcggacgttt tgtcccagaa
     7321 acattgatga cagcagtttt ggagttggag aaggcctacc gtgaaagtca ggcagaccca
     7381 agtttccaag aggaattaaa ccaacttttg cgccagtatg tgggacgtga aactcccctt
     7441 tactacgcaa aaaacttgac ccagcatatc ggcggagcca agatttatct caaacgggaa
     7501 gaccttaacc atacaggagc ccacaagatt aacaatgcct taggacaagt ttggcttgcc
     7561 aaacgcatgg gtaaaaagaa aattatcgca gaaactggtg ctggtcagca cggtgtggca
     7621 actgcaactg ctgcggccct ctttaacatg gaatgtacca tctacatggg tgaggaagat
     7681 gtcaaacgcc aagccctcaa tgtcttccgt atggagcttt tgggagctaa ggtcgaggcc
     7741 gtgacagatg gttcgcgcgt gctcaaggat gcggtcaatg cagctcttcg ttcatgggta
     7801 gctaatatcg atgataccca ctatatcctt ggttctgcct tggggcctca tccatttcca
     7861 gaaatcgttc gtgacttcca aagtgtcatc ggtcgagagg ctaaacaaca gtaccgtgac
     7921 ttgacaggtc gagatctgcc agatgcccta gtagcctgtg ttggtggtgg gtcgaatgct
     7981 atcgggctct tccatccctt tgtagaagat gagtcagtag ctatgtatgg aactgaagca
     8041 gcagggcttg gtgtggatac agagcaccac gctgctacct tgaccaaggg tcgtccggga
     8101 gttcttcacg gttctctcat ggatgtgctc caagatgccc atggtcaaat tcttgaagcc
     8161 ttctctatct cagcaggttt ggactatcct ggtattggtc cagaacattc tcactaccac
     8221 gatatcaaac gtgccagcta tgtccctgtg acagacgaag aagccttgga aggattccaa
     8281 ctcttgtctc gtgtggaagg gattatccca gccttggaat ctagccatgc tatcgctttt
     8341 gcggtgaaat tggccaaaga acttggacca gaaaagtcta tgattgtctg cctatcaggt
     8401 cgtggggaca aggatgtggt tcaagtcaaa gaccgcttgg aagcagatgc agcaaagaag
     8461 ggagaagctc atgcctaaga cactaacaga aaaattaaat gctataaaag cagctggaaa
     8521 aggaattttt gttccctata tcatggctgg agaccacgag aaaggtttgg atggactagg
     8581 agaaacaatc cactttctag aagatttggg tgtctctgca attgaagtgg gtattccctt
     8641 ttcagaccct gttgcagatg gacctgttat cgaagaagct ggcttgcgca gtttagccca
     8701 cgggacctct acccaggctt tagttgaaac cttgaaaacc attgaaacag agattccact
     8761 ggtcatcatg acctacttca accccctctt tcagtacggt gtggagaact ttgtcaaaga
     8821 tctggctgat acagctgtga agggcttgat tatcccagac ttgcctcatg aacatgccaa
     8881 ctttgtagaa ccatttttgg cagatacaga tatcgccttg attcctttag taagcttgac
     8941 cacaggaatt gagcgccaga aagagttgat tgaaggggcg gagggattcg tctatgccgt
     9001 tgccatcaat ggagtgacag ggaaatcagg caattaccgt gcagatttgg acaagcactt
     9061 ggcgcaactt catcaagtgg ccgacatccc agtcttgaca ggttttggtg tatctagtca
     9121 agccgatctg gaacgcttca atgcggtgtc agatggtgtt atcgtcggtt caaaaatagt
     9181 aaaagctctc caccaaggag agccgattca ggattttatc agacaagcag tagcttacca
     9241 aaaataatca aacaagtagc aagt
//
LOCUS       DAVHIT010000082         9087 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVHIT010000082 DAVHIT010000000
VERSION     DAVHIT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.084"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            2..835
                     /locus_tag="VVN16_001712"
                     /pseudo
     CDS             2..835
                     /locus_tag="VVN16_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            828..1541
                     /locus_tag="VVN16_001713"
     CDS             828..1541
                     /locus_tag="VVN16_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5573049.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(1863..1955)
                     /locus_tag="VVN16_001714"
                     /pseudo
     CDS             complement(1863..1955)
                     /locus_tag="VVN16_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2276..2977
                     /gene="pyrF"
                     /locus_tag="VVN16_001715"
     CDS             2276..2977
                     /gene="pyrF"
                     /locus_tag="VVN16_001715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU5573050.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIYYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            3011..3643
                     /locus_tag="VVN16_001716"
     CDS             3011..3643
                     /locus_tag="VVN16_001716"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5573051.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLSKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            4321..5085
                     /locus_tag="VVN16_001717"
     CDS             4321..5085
                     /locus_tag="VVN16_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573052.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            5085..5837
                     /locus_tag="VVN16_001718"
     CDS             5085..5837
                     /locus_tag="VVN16_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573053.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTAILFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            5847..6545
                     /locus_tag="VVN16_001719"
     CDS             5847..6545
                     /locus_tag="VVN16_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573054.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            6542..7189
                     /locus_tag="VVN16_001720"
     CDS             6542..7189
                     /locus_tag="VVN16_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573055.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7269..8134)
                     /locus_tag="VVN16_001721"
                     /pseudo
     CDS             complement(7269..8134)
                     /locus_tag="VVN16_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(8146..8903)
                     /locus_tag="VVN16_001722"
                     /pseudo
     CDS             complement(8146..8903)
                     /locus_tag="VVN16_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(8904..>9087)
                     /locus_tag="VVN16_001723"
     CDS             complement(8904..>9087)
                     /locus_tag="VVN16_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HEU5573056.1"
                     /translation="LVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVC
                     YPISKLSTHLEKHWRN"
BASE COUNT         2687 a         1465 c         1842 g         3093 t
ORIGIN      
        1 tatgattgaa gttagccatt tatcaaaaag ttttggtgat aaaatagctt taaataatat
       61 aagcttcact gttaaagaag gttagatttt tggattttta ggaccatctg gttctggaaa
      121 gaccacaacg attaatattc tgactgggca gttccttgcc gataaaggac aatctattat
      181 tttgggacaa aaatctcaaa atttaacaag cggtgaatta aagagaattg gattggttag
      241 cgatacaagt ggattttatg agaaaatgtc tctgtataac aatcttcttt tttatagtaa
      301 attttataat attagtaaat cacgtgttga taatttgtta aagcgagtag gattatatga
      361 tagtcgcaag atggtagcag gaaaattatc cactggaatg aggcaacgaa tgcttttagc
      421 acgagctctt atcaacaaac ccgctgtact ctttctggat gaaccgacct caggtctaga
      481 tcccacaact tctcgaacaa ttcatgagtt aattttagaa ttgaaaacag cagggacaac
      541 gatttttcta acgactcatg atatgaatga agcaactctt ttatgtgatt atgttgcctt
      601 attaaataaa gggaaattag ttgagcaagg agctccttct gaactcattc aaagatataa
      661 taaagataaa aagattaagg ttacagatta taatgggaat cagataactt ttgattttac
      721 atcactagaa caggtatctc aggctgatct ggaaaatatt ttttcaattc attcatgtga
      781 gcctacttta gaagatattt ttatcacatt aacaggagga aagctaaatg cttaaacggt
      841 ttctggcttt ggtatggttg cgttgtcaaa ttatcctttc caataagagt attttattgc
      901 aagttttagt gccttttgct ttcacatatt tttataaata tcttatggaa acacagggga
      961 aggtcaacga tcaacaggca ttagttcttt tgatgatgtg tttacctttt tctttggctt
     1021 tggctgttgg aagtcctata actattatct tgtctgaaga aaaagaaaag tacaatttac
     1081 aaactcttct gttgagtggt gttaaaggat ccgaatacat tttatcaacg atgtttcttc
     1141 cttttttgct aacttttgtg attatgggaa ctactcctct tattttagga gttacaattg
     1201 tacatacttt taattatatt acaatcgttc ttctaacctc tttatccatc attttattct
     1261 atttattgat aggtttaacc gcgaagagcc aagtagtagc tcaggttatc agtcttcctg
     1321 ctatgatttt agttgctttc ttaccgatgc tatctggttt ggataagaca gttgcgaaga
     1381 taacagatta tagttttatg ggactgttta ctaagttttt cacaaaatgg gagggatttt
     1441 catggaatga aactctaatt cctaatctaa cactacttat ttggattgtt cttctattaa
     1501 ctttaattac gataactatt aggaaaaaga aaatttctta attgagttat tttaatgatt
     1561 ataaacacaa gtgggaagga aaaatgaact gatctttttg acagcaattc tacagaatag
     1621 tcttattgct atattttgat ttgagtgtac gaaaaaagaa aaataacaat agtgctcata
     1681 ctaattgcag aagttttggg tgataagata actgataaat tgcaataaaa aatgcaacat
     1741 ttttaaatct cctctataag tgcttcaaaa cctgtcttgt aatccaagta tttttgggga
     1801 cggtgattaa taagctagca aagcaccatt aaggattttt tcggtaattg ttgccaaatc
     1861 ggtttaagaa aatactcacg aagaagtcca ttcgcattct cattacttcc cctttgccaa
     1921 gatgaatagg catccgcaaa ataaaacaga attcccattt gttcaattaa agggtaacaa
     1981 gcaaactctt tttctctgtc ctaagtgaaa gtctttaact attcttttgg aaagagtctt
     2041 gtgaggtgtt caatagcagt caacacatac gtgaccatgt ttttacttga caagtgctag
     2101 tagaaataat agaataatag aaaaccttta aagcagtcca gagaggcagc taaggttaga
     2161 cggtgaaagg gtggagacta cccatttttc gtggaacctt gctgttggca ggttcctttt
     2221 ttcgtggctt ctgttggcca gactctctca ctagtaaagg taaaaggaga aacctatgcg
     2281 agaacatcgt ccaatcattg ctcttgattt tcctagtttt gaggcggtca aggaattttt
     2341 agctcttttc ccagcagaag aaagccttta tctcaaggta gggatggagc tttattacgc
     2401 agcggggcct gagattgtgt cctacttaaa aggtttgggt catagtgtct ttttggatct
     2461 caaacttcat gacattccta atacagtcaa gtcagccatg aaggtcttgt ctcagcttgg
     2521 tgtcgatatg actaatgtcc atgcggctgg tggtgtagag atgatgaagg cggcgcgtga
     2581 aggtcttggg agtcaagcta aattgatcgc tgtaacgcag ctgacatcaa cgtcagaagc
     2641 ccagatgcag gagtttcaaa atatccaaac cagtctgcaa gagtctgtga tttactatgc
     2701 caagaagaca gctgaagctg gcttggatgg tgttgtttgc tcggctcagg aagtacaagt
     2761 catcaagcag gctaccaatc cagattttat ctgtctgaca ccagggattc gtccagctgg
     2821 tgctgcagtt ggagatcaaa aacgagtcat gacacctgct gatgcctatc aaatcggcag
     2881 tgactatatc gtagtgggac gtcccattac ccaagctgag gatcctgttg cagcttatca
     2941 taccatcaag gatgaatgga cacaggactg gaattaaaga actagattag aaaaataaaa
     3001 ggagaatacc atgacacttg ctaaagatat cgctagccac ctcttgaaaa tccaagccgt
     3061 ttacctcaaa ccagaggaac ccttcacttg ggcatctggt atcaagtcac cgatttacac
     3121 tgataatcgt gtgacactag cctatccaga aactcgtacc ctaattgaaa atggttttgt
     3181 ggaagctatc aaagaagcct ttcctgaagt agaagtgatt gcaggaactg caacagcagg
     3241 gattccacac ggagccatta ttgctgataa gatggacttg ccttttgcct acatccgtag
     3301 caaaccgaaa gaccacggag ctggtaatca aatcgaaggt cgcgtagctc aaggtcaaaa
     3361 aatggtagtg gttgaagacc ttatttcaac gggtggttca gttcttgaag ctgtagcagc
     3421 agccaagcga gaaggagcag atgtacttgg agttgtagcg attttcagct accaattgtc
     3481 aaaagcagat aagaactttg cagatgctgg tgttaaactt gtgacgcttt caaactatag
     3541 cgagcttatc catctagccc aagaagaagg ttacatcaca ccagagggcc ttgatcttct
     3601 aaaacgcttt aaagaagacc aagaaaattg gcaagaaggt taggtcagta agataaagag
     3661 agacgaggct accgagtctc ttttaccatt ttatttaaaa tatgacaata ttttaaataa
     3721 aataattgac aacgctttct tttattgata taatgaaaat gtaatatgta tcaaattgat
     3781 caatcaacta tcttgtttat cacttcttaa gaggacgatg tcatgaaaag aagaaaaata
     3841 tattcctttt ttgcgtagtt acaattgcat ctagtttgtt tctccctaca gttttagcta
     3901 gacagattgg aaattatgat ttaacgttgc cgcgttgggg ttcggataca actagtgagc
     3961 tgagaaagaa aacgcctctg ctggaattaa taataatgac agtactggtg gcggtaaaac
     4021 attaaatacc tctattcgta gcgcctatag tgggtcagat attaccccgg tatattcatt
     4081 ggggtctggc tctaggattg tcatatacta taatggaggt ggtgacaatt atattggttc
     4141 tggtactaga ttagctatgg cgccacaatt tggaaatcat gtaagaattc atacttcagg
     4201 ttcttggagt ccagattctt attaacttgc ttgtcagagt aagccttaaa gatggttgat
     4261 tgtgggtgta gcatgaaaaa agaatgctac accctatttt tattataagg aggagtaagg
     4321 atggaatttt tcatttgtaa tcttgtacga gtcgttcaat cacctcgatt ttatatgtct
     4381 ttatttttga cccttctttg catgagttta ggaaatttcc ttgctttcaa tggtatttat
     4441 aaaattgaag gtttatcgat tttttttgcc gcttcttcta ttcgaggatt ttcaccgatt
     4501 agcctagtag ctgcacttat ctgtacactg ccctattcta gtcagataat agaggatgct
     4561 gagagtcatt ttctaacagc acaattgtgc cgaatttcta aaaagaggta tctagctatt
     4621 gtaggtagta ctgcaattat ttcttctttt ctagtctttt ttctccccta tttattatta
     4681 ttaggaatta atcttttagt gactccttat caggaaattt atattggaga ttatagtggt
     4741 gccttaaaag aattatttga ttctaatcag ttgctctata gtcttgtgac gactctctgg
     4801 tatggagttt ggggcgctgt gttctctatt tttggactag ctagtgcttt gctagtgaag
     4861 aaaaaaatag gagctatttt catcccagtt gcctatatga tggttggtgg tattttttgg
     4921 gctattttag ggctatctta cttagaacct gtgacaacat tagctttggg atatcagaaa
     4981 gatatcagtc tttccttagt tagtgggcat cttgctttta ttttatttgt tagttgtttg
     5041 gttgtttatg gtacattttt tctacattca gaggactatg tataatgaaa caatttgttc
     5101 aattttataa aaaagatttc ttagcagtat tggtttattt tatattactg ctatcctgtg
     5161 ttttatctag tacagtatat ttattgcgct gtcgccaata ttcaatccat ccaaatgtat
     5221 tagaatggat cttagtttta cttcaagata tgacgactgg agtatattgc tttccgttca
     5281 catatatatt gttctttttt tatttgatga ataactattt taataggttg gagtgtcgca
     5341 ttcgtctgaa atcaattaag cactttacca gttttagttt caaattagca gctcttagta
     5401 cggggatttg gacggcgatt ttatttttat tgatttttct aattgcattt agtaatggtt
     5461 ttagcttctc tttggagata aaggaggttg attttttaag agaattttat ggcataagta
     5521 ttgcaaacaa tgctagtttc tttataggat tttttttctc ttatatagca tactatttct
     5581 ttttatcctt acttactatt agcagttttt cttggtttaa aaaatcaaac atgagcttag
     5641 tatttctgtt tactttttta tttgtagaat ccttattctg gatttatcag ttggacaatg
     5701 ggataattgg attattgcca atttttcagt atatggtaaa ttccaatccg tatgcattga
     5761 tttattggct tacattacta tctatcataa ttccattgac tgtattttct gttcatagaa
     5821 actggaggag agtgtaaaag ttggaaatgg gaaagttaag tagtcacatg tggaggttga
     5881 atcagataat ctataccaag tacttttggg gttatgttct tttttggata ttgatttgtt
     5941 taggattatg gtattggtta gaaggaaatg atagacttgt tatagaaatt ttaaaagggc
     6001 ctaatctgag tcaaaactct tttttagtct tatctatatg gttgcttcat tggtttatta
     6061 ttcatacatt ttttctagca gttgtatatc gtagaagagc atccgatttc tttatggaag
     6121 tgattcgatt ttcttctatt aagctctgga ttaggtatca gatttggacc tgttttcttt
     6181 atggactcat tttaatcatg gtaaaagttc tagtgattca atttatgtta cagttaccaa
     6241 actgggatat aggagttttg tttatagttg attctttgaa tgcttgtgtg ttagtcttgt
     6301 tttgctttat gttatacgca ctaggagcga atgtacaaat gaactttgct tgcgttagtt
     6361 tctttttact catgattgtg tttggtggtc tatttgtagg aaatcgaact aattatctat
     6421 tttatatttt gaatcgaggg aatggagata taggacgaga tttatttctt cagttgctat
     6481 ttttagtctt tctttttaaa agtattttct atttcactcg tcaaaagagg aggtttatag
     6541 aatgaatgaa ataatcatat taaaaaatat tgagttgaaa ttaaaaaaaa catgtgtttt
     6601 tcaaaacctt aattttagtt gtaaacaggg ggaaattata ggaattactg gtgcgaatgg
     6661 ctcagggaaa agtgtattgt ttaaattaat agctggttta tatagtccgt cttatggaga
     6721 agtgttaatc aatggggaaa atattgttcc tgagagaaaa attccagcta atttgggagc
     6781 tttgattgaa gaacctggtt ttataaatta ttatagtggc tttaagaatt tacaatattt
     6841 ggcaagcata cgaggagtag ttggtaatca ggaaatcaat gatacactga aaatagttgg
     6901 tctatatgag caaaaagacc agaaagttaa aacttattcg ctaggtatga ggaaaaagct
     6961 agggattgct caagcaatta tggagaatcc ctctattctt ttactagatg aacctatgaa
     7021 tgccttggat aaatcaagtg tagaaaatat gagaacattg tttagaaagc tctctagtga
     7081 aaaagggaca acaattttga ttgctagtca cagtgaagag gatattcgta tcttatgtga
     7141 taaagtatat gcaatagaag ataaagtatg tacactgtgt tcagattgaa catcttagat
     7201 agactcagga tgttataggt aaaatcttaa aaagggattg gagcttaaaa tccagtccct
     7261 tttaagagtt aatggccacc ttcaacaacc aggtcatctg ctttagcagc gtcaccgtag
     7321 gttgggtgaa gtgttttttc ataggccttg tggaagaagt tttccttgcc taatttttca
     7381 atatctttat tgatgaagtc tagcaattct tggttgcctt tttgaactgc tgccgcaatg
     7441 gtatcgggat caccgaggga agtaattcct acttcaaatc ctttattttc aagcgcccaa
     7501 gctagaactt ccgtattgtc agttgaaaag gcatctccac gtccgtcaag aagagcttgg
     7561 taagagtcac tgtattggtc gtatttttgg agtttgattt ctggatgatt cttttcaaaa
     7621 taagtctcag cagtcgttcc ttttgtgaca attaaggttt taccttcaag ttgtttgacg
     7681 tctgtaatga gaccagtctt aggtgatacg acacccagag aaactttcat atatggaagg
     7741 gcaaaatcaa cttgtttctt acgttcgtca gttactgtaa agttagcaag agtaatatct
     7801 accttgtttg aaatcaagta ttccgcacgg ttgtcagcat cgactgaaat gtatttaacc
     7861 ttgacaccaa ggtcttgagc tagttggttc cctagttcaa tatcgtagcg tagccttggt
     7921 aagaaccatc attgtcaacg tagccaaacg gttttttatc tccaaacacg gcgattcgca
     7981 gttcaccgct ttttttgatt tcatcgatag tgcgagcttt ggcagttgtt tttccagagg
     8041 atgaaccagc gtttccacct gagctacaag cagtgataaa gataagggca aaggcaagtg
     8101 ctaaaacagt taagagtggt ttgaatagtt tcataagaat ctcctttata gatatgagcc
     8161 gaattggcta aagtcaaaga cgtttaaaaa ttcctgggct cgtttggttt gcggattggt
     8221 aaagaaggct tgagctgttc cttcttcagc gattttccct tggtcgagga agataatctg
     8281 gtcagcaatg gcttgggcaa actgcatttc gtgggttact aaaatcatgg tacggccttc
     8341 ttgggccaaa tcattgataa gttccagcac ctcacgcacc atttctggat ccagcgaagc
     8401 agtcacctcg tcaaaaagga tgatttctgg atgcattagg agggcacgga caattgcaac
     8461 acgttgcttc tgtccaccag ataattgacg ggcaaagcta tgttgtttat ccagcaaacc
     8521 gacacgctct agtaattgca aagcttcttc cgttacttct ttcttgtccc ttccttgagc
     8581 tttgataggg cctaggatga ggttttgtaa gacatccaga tggggaaaga gttcataact
     8641 ttgaaagacc atgccaatct tttggcgaac taggtgaaaa tcttttttat tttcaacgat
     8701 agactgacca tccagaagaa tatctccacc ttgaatactt tctaagccgt tgaggcaacg
     8761 aaggagggta cttttcccac aaccagaggg ccctaggatg acaacaactt cccttttttg
     8821 atttctagag aaagtccttg gaggatggga ttgtctccga aggatttttt tagttccttg
     8881 atttctaaga tagtttcaga catttagttt ctccaatgtt tttctaagtg agtggatagt
     8941 ttggaaatag ggtagcaaac tgcgaaatat aagactagaa tggttccata aatccaaaat
     9001 gaagcagttg ggatggtcag gcgattgcta tcgatgattt gttgtccaac tttggtcact
     9061 tccacaaccc caatcaaaac aactaat
//
LOCUS       DAVHIT010000083         8945 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVHIT010000083 DAVHIT010000000
VERSION     DAVHIT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.085"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(346..1194)
                     /gene="dprA"
                     /locus_tag="VVN16_001724"
     CDS             complement(346..1194)
                     /gene="dprA"
                     /locus_tag="VVN16_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU5573057.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1290..1979)
                     /locus_tag="VVN16_001725"
     CDS             complement(1290..1979)
                     /locus_tag="VVN16_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU5573058.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1991..2869)
                     /locus_tag="VVN16_001726"
     CDS             complement(1991..2869)
                     /locus_tag="VVN16_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5573059.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2859..3728)
                     /gene="licA"
                     /locus_tag="VVN16_001727"
     CDS             complement(2859..3728)
                     /gene="licA"
                     /locus_tag="VVN16_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU5573060.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEYFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3745..4767)
                     /locus_tag="VVN16_001728"
     CDS             complement(3745..4767)
                     /locus_tag="VVN16_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5573061.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4772..5479)
                     /locus_tag="VVN16_001729"
     CDS             complement(4772..5479)
                     /locus_tag="VVN16_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5573062.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5815..7302
                     /gene="tacF"
                     /locus_tag="VVN16_001730"
     CDS             5815..7302
                     /gene="tacF"
                     /locus_tag="VVN16_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU5573063.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7312..8115
                     /locus_tag="VVN16_001731"
     CDS             7312..8115
                     /locus_tag="VVN16_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU5573064.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8117..8926
                     /locus_tag="VVN16_001732"
     CDS             8117..8926
                     /locus_tag="VVN16_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU5573065.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHHRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHNIKAYRKN"
BASE COUNT         2806 a         1807 c         1507 g         2825 t
ORIGIN      
        1 cacatcttat ttcaattcac tatactttcc cttatactca atgaaaatca aagcgcaaac
       61 taggaagcta gccacaggtt gctcaaagca ctgctttgag gttgtagata agactgacga
      121 agtcagctca aaatactgtt ttgaggttgc agatggaagc tgacgtagtt tgaagagatt
      181 ttcgaagagt attatatgaa aaaatcctct actctactaa tccacttaga aaaaacgcat
      241 attatcaagt gttaaaaact tgctaatatg cgttttatta tggaaatgtt gggttcattt
      301 tctcttaaaa ttgatttttt cttagaattc tagcttaggt catttttaaa attcaaattc
      361 cgcaagaaca tcttgcccac tggtgaccaa ttttgctcct tcttgaatca aatgatggca
      421 accgtctgat agtccatcta aaatgctacc aggaatagca aagacatcgc gtccttcttc
      481 cattgctcgc tcacaggtaa tgagactacc tgaacgcatc ttagcctctg ctacaatcac
      541 accacgacaa agtccagcaa tgatgcgatt acgggcagga aaatgaaatt tcagaggttg
      601 ttcgccaggt ccatattcac ttagagccag atggtcattg ccgatgtagt cttgcaagcg
      661 tttattggct ttaggataaa acacatccag tcctgttcca atcactgcaa tggtttttcc
      721 gccattctga agagctgcca tatgagctgc tgtgtcaatg cccttggcca gaccactgac
      781 aataaccagt tcattttcca agccttgaat gactttttca actgacttag ctccctgttt
      841 gctacaagca cgactgccca cgaccgctac cttcgggaat ttcaggaggt caagatttcc
      901 cttgtaaaat aaaagtacag gcgcatcata tatttcactc aaatcccaag gataacagtc
      961 atctaaaata gagaaagatg gaaatttttg aaactctttc gacaaatgcg catcgtctat
     1021 ctgaaaataa cgttccataa aaacggctgg attacggcaa cctgatatat ctgcaatatc
     1081 acccaacaaa agctcctgat caacattttc accgtattct agcactttca aaatctgttg
     1141 attggtcaaa cctgattttt ttaacttata gatttcatag tttgtgattt tcataaataa
     1201 ctccatttct ttttctactc atctatctat tcgtaaaagt tcgtaaaaaa tcaaaaaaca
     1261 tccgactatt ttgtcagatg ttggaatctt taattttcgt ttttaagaat ttcttctaat
     1321 ttacgatagt cttggacact atcgatctca taaatgctat tgccttctaa ttcttcaaca
     1381 tagacatcta gctctttgat attatcctta accatattgt cccaatagag atcaacaaat
     1441 tcaccacttg cataagcctt gtcgataaag ctgacaatct tttctgcagt tggagcatcc
     1501 cagaaggata caccactaag gatgcgacct gccttgctat caacaataat gtcttgaacc
     1561 ttgtagtcat ctccatagac caagaaccat tcgttggtac aatcttcacg ataaacacta
     1621 aaataagtcg aacgtgtcaa atcattgcgg aacatatttt taaagagata attgtcagca
     1681 tcaataacat agctgttggc caattcttct tttacaagat agagagagta aaagttattg
     1741 tagtcagcgt atttatcatt gaaaacgagg cgaacaccgt atttttcttt taaataatcg
     1801 aattgttctt taagataacc aacgatgatg atgatgtcat tgattccttt ttctttgaga
     1861 aactcaattt ggtattcaat caaaggtttt tgattaacct gaaccaaggc tttaggggta
     1921 ttttcagtca taggacgcaa gcgagttccc aatcccgctg ctaagataat ggctttcaca
     1981 cgaatctcct ttattcttta ataataatat aaactccagc aatgacgaca agtgacgtca
     2041 taatggtcag catatctaat gatgttccca agaaaagagc tgcgaacaag acagtccata
     2101 ctacatagct cacgttcaag cctgtagcct tggctggttg caagcgattg atagcgatat
     2161 aataagccaa ataggaaatc atatcaaagg ctacaaagac aatcatgaga cctagcaatt
     2221 gtccattggc tactgcaaca aatgactgat gagagaagag cacaatcaca agataggaca
     2281 agaacgaagt tacttgacgg attaagaggg cttcgatttc actcaattca ctttccatgg
     2341 caaaagagct aagaacactc tcacttcccc atgcaatagc acaaaccaaa gcacaaagaa
     2401 tcccaatgta gaaagaatta acctgttcaa ccttataggt ctgggcaata atccctccaa
     2461 taatcaagac aatcccaaat acagtatttt tcgaaatctt gtgcttcaaa aagaagaaag
     2521 ccaatagaac tgaaatcgca gggtaaatag ccgatacaga tgaagctaaa gaacttccga
     2581 tatacttaac tgcataaaga ttggcctgca taccgatagg gcctgctagc aaggctccta
     2641 tgataacact gacattgcga atatttaaga aaattgagag gcgaactttc ccttctttta
     2701 ccaagagaaa agctagtaag ataaagatgc tcaaaaaatc atgagttgca gccaccacaa
     2761 agggtgacaa atctgtaaaa atcgaaaaga tataagcact aaccgttaga cccaagcccc
     2821 agaaaatacc tgagagaagg ccaaaaggaa ctccgttttt acttttcatc tgaacctcca
     2881 taagaagcca aacctttaat agctctttgg taacgattca caccatagtc accaaaatat
     2941 tcaccttgct cttccttata gacagtccat agactccaaa tagtatcttg taaaatttta
     3001 taaatagcaa tcttttcatg agaaaccggt gtttggtcac tctcatagtg agataagaaa
     3061 gtttcttcct cttgggaagt gaattcagac tctaaaaaga gggcagccaa atcccacatt
     3121 ggatcattca ttgatgaata ttcccagtca atcaaataaa gtcgtccttg aggtgattcg
     3181 ataaagtttt caggcaccaa atcgatatga caagattttc tgtcaacacc taagtcagcc
     3241 agtcttttct ctaaggagaa gactgcattt ctaacagatt catagttggc ataaggaatt
     3301 tgttcttcaa tcaaggattc gtattttttg atttcttcaa aaggagcaaa ttctcctctt
     3361 aattccttag cagacgtatg aatagtttgt aatattggag taattttgtc gaacttggtc
     3421 ttgattgacg ttgaatcaag cgtaatcgca gattcgatat actcatttac tttgatacca
     3481 gcttcaatat caaaaagata attttttaca tctaagccta aatcctttag tagttcaaga
     3541 ttgtactttt catcttgtcg attgataagc ttttctgtcc ctttaccaaa gaatttaaca
     3601 atgtattgct tatttgttgt tttggccaaa tagttttgat tggtcattcc acccagttgt
     3661 tcaacactga ggacttcctc ttcttgacta agtaaggaag aaattttttc tttaatgatt
     3721 ttctccacaa ttaacctcca gtacttatac ttcccactta aacactgttt taaaggctgt
     3781 gtttaaatcg gttgcaaaga cacgatgaat atctttaatt tctcttacag gttcttctag
     3841 ataaaggata tttttaagac gattggcaaa tttcttgact tccatcattt ggatagcatt
     3901 ttcaaaatca atgcgaccag aacgagatga cccaaccaaa atcaagccct tttctaaggc
     3961 atcgcgagta ttgagattga ctttatattc gctaactccc atcatgagaa tcgttccctg
     4021 aggacgaatg tagcgaatca agtcattaat agctggtcca gtaccatcac caccacaaca
     4081 ttcaaaagca tggtcaaagg ccaaatcttc aggaatatta tccgtaatat agcattcttt
     4141 ggcaaatgag aagagttcca acttttccca atgacgacca ataaccacaa tctctgcttc
     4201 tggcaaagta tagttgataa tattggcaac cacaaaagct aaacttccat ctccaataac
     4261 ggcgatccgc tcccgcttgc tatgagcaag agtcaataga cgattcatag cgtgcatgcc
     4321 cacactgaca aactctgtaa tggctgcaac cgtatcttca atagcatcat aagccaccac
     4381 acgatcttta gggagagaaa caaactctct cataaagcca tcaaatccac tagacaagaa
     4441 atgggtccct gtcatgtagt tttcatagaa ttcttcatca ctctgcatag gagactgatt
     4501 gggaatcatg acaacttttt gaccaacctc gtaggttccg gtcgggtcag aaatgacggt
     4561 tccacatgac tcgtgaatca ttgccattgg aagcttttta ttcaaaatct tgggatcacg
     4621 ttttccctga tagtaacgct gatccgcatg acagacagcc atgtagttgg gacggataag
     4681 gatatgattc tcttggtcaa tagcctcttc ctgatatttg acattgataa acttaggctt
     4741 agttagttga taaatttgat taatcatttt actagtcttt ctcaatcata ctttttgcaa
     4801 tcttcaaatc tgttacggtt gtaatcttca gatttgagta ttcacctttg gccaaggcca
     4861 catcttttcc tttgatcaca aagattttac atgcatctgt caagatttcc ttctcttcat
     4921 cagaaagaga tccataaagg tccatgaagt ccttgcaacg gaatgtttga ggtgtttgtc
     4981 cttgataaag gtgagcacga tttggaatat ctgtaataaa ttgaccattg gtactttcaa
     5041 cgatagtatc aaccgcttct accactgtgt ccactgcgtc atgattttgg gcaagttgga
     5101 tattgtcctg aatcatgcga agtgtaataa atggacgaac agaatcgtgg gtaacaacga
     5161 tatcctctgg agtaagcgga cgataagcat caatggcttc aatgattttc ttaatacttg
     5221 tattgcggtc agcaccaccc tttgtaatga tgatacgttc cttataaaga ggaagatatt
     5281 tatctacaag atcttctgca tgagaaaccc agtctccatg aacaccaact acaatttttt
     5341 caatacttgg ctccaagaca aatttttcaa ttgtatgaat caaaataggt cgatcaccta
     5401 gctctaaaaa ttgttttggc aagttactga tccccatgcg tgtgccagtt ccaccggcaa
     5461 gaattcctgc ataaatcatc tattttctcc tttgtcttac taaaaaaact tattctctct
     5521 attataccac aatctagtcc tatcataaaa gaaatactgg tcctcccctt ctagaatagg
     5581 agaattatgc agttctatta ttcaaagaaa ctagtccatt tctagtaatg actaggaatt
     5641 ctgtagcaat tactaataaa tggctgcgaa attttagagt ccttcagaat aatatacttt
     5701 ccttaaagaa aacttaaaac ttaagatttg aaacttcaaa gtgattactt gacttactgc
     5761 ccttcatttc ttattagcta actttatgat ataatgaaaa acgaggtaaa ttgaatgaaa
     5821 agtataaaat taaatgctct atcttacatg ggaattcgtg tcttgaatat tatttttccc
     5881 atcctaactg gaacctatgt cgcgcgtgtc ttggaccgaa ctgactatgg ttacttcaac
     5941 tcagtcgaca ctattttgtc atttttcttg ccctttgcaa cttatggtgt ctataactac
     6001 ggtttaaggg ctatcagtaa tgtcaaggat aacaaaaaag atcttaacag aaccttttct
     6061 agtctttttt atttgtgcat cgcttgtacg attttgacca ctgctgtcta tatcctagcc
     6121 tatcctctct tctttactga taatccaatc gtcaaaaagg tctaccttgt tatggggatt
     6181 caactcattg cccagatttt ttcaatcgaa tgggtcaatg aagctctgga aaattacagt
     6241 tttctctttt acaaaactgc cttcatccgt atcctgatgc tggtctctat tttcttattt
     6301 gttaaaaatg aacacgatat tgttgtctat acacttgtga tgagtttatc gacgctgatt
     6361 aactacctga ttagttattt ttggattaaa agagacatca aacttgttaa aattcaccta
     6421 agtgatttta aaccactctt tctccctctg acagccatgt tagtctttgc caatgccaat
     6481 atgctcttca cttttttaga tcgcctcttc ctcgttaaaa cagggattga tgtcaacgtt
     6541 agttactata ccatagctca gcgaattgtg accgttatag ctggggttgt aacaggtgca
     6601 attggagtga gtgtgcctcg tctcagttac tatctgggga aaggagacaa agaagcctat
     6661 gtttctctgg ttaatagagg tagtcgaatc tttaacttct ttatcattcc actgagtttt
     6721 ggactcatgg ttttaggacc aaatgccatc ctactttacg gtagtgaaaa atatatcgga
     6781 ggcggcatct tgacctctct cttcgctttt cgtacgatta tcctggcctt agataccatt
     6841 cttggttccc aaattctctt taccaatggc tatgaaaaac gtatcacagt ctatacagtc
     6901 tttgctgggc tactcaattt gggcttgaat agtctcctct ttttcaacca tatcgtggct
     6961 cctgaatact acttactgac aactatgtta tcagagactt ctctacttgt tttctatatc
     7021 attttcatcc atagaaaaca actcatccac ttgggacata tctttagcta tactgttcga
     7081 tactctctct tttcactttc ctttgtagca atttatttcc tgattaattt catgtatcct
     7141 gtggatatgg tcattaattt gccatttttg attaatactg gtttgattgt cttgctatca
     7201 gctatctctt atattagtct acttgtcttc acaaaagata gcattttcta tgaattttta
     7261 aaccatgtcc tagccttaaa aaataaattt aaaaaatcat aggagtttaa aatgaaacaa
     7321 ctaaccgttg aagatgccaa acaaattgaa ttagaaattt tggattatat tgatactctc
     7381 tgtaaaaagc acaatatcaa ctatattatt aactacggta ctctgattgg ggcggttcga
     7441 catgagggct ttatcccttg ggacgacgat attgatctgt ccatgcctag agaagactac
     7501 caacgattta ttaacatttt tcaaaaggaa aaaagcaagt ataagctcct atccttagaa
     7561 actgataaga actactttaa caactttatc aagataaccg acagtacgac taaaattatt
     7621 gatactcgaa atacaaaaac ctatgagtct ggtatcttta tcgatatttt ccctatagat
     7681 cgctttgatg atcctaaggt cattgatact tgttataaac tggaaagctt caaactgctg
     7741 tctttcagta aacataaaaa tattgtctat aaggatagcc ttttaaaaga ttggatacga
     7801 acagcctttt ggttgctcct tcgaccagtt tctcctcgtt attttgcaaa taaaatcgag
     7861 aaagaaattc aaaaatatag tcgtgaaaat gggcagtata tggcttttat cccttcaaaa
     7921 tttaaggaaa aggaagtctt cccaagtggt acctttgata aaacaatcga tttacccttt
     7981 gagaatttaa gccttcctgc acctgaaaaa tttgatacta ttttgacaca attttatgga
     8041 gattatatga ccctaccacc agaagaaaaa cgcttctaca gtcatgaatt tcacgcttat
     8101 aaattggagg attaggatgc aatatttaga aaaaaaagaa attaaagaaa ttcaactagc
     8161 cctgctggac tatattgatg agacttgtaa gaaacatgat attccttatt ttctcagtta
     8221 tggaaccatg cttggagcca tccgccacaa aggtatgatt ccttgggatg atgatattga
     8281 tatttccctt tatcgtgagg attatgagcg tttactgaag attattgaag aagaaaatca
     8341 ccatcgctac aaggttcttt cctacgatac atcttcttgg tacttccata atttcgcatc
     8401 gattttggac acttctactg ttatagaaga ccatgttaag tacaagcgtc atgataccag
     8461 ccttttcatc gatgtcttcc caattgatcg atttacagac ttgagcattg tcgacaagag
     8521 ctataagtat gtggcccttc gtcaactagc ttatatcaaa aaatcacgag cagttcacgg
     8581 tgatagcaaa ctaaaagatt ttcttagatt atgtagctgg tacgctctcc gatttgtcaa
     8641 tcctcgctac ttttacaaga aaattgatca actagtcaaa aatgctgtaa ccaacactcc
     8701 tcaatatgaa ggaggagttg ggatcggtaa ggaagggatg aaagaaatct tcccagttga
     8761 tacctttaaa gaactgattt taactgagtt tgagggccgt atgttgcctg ttcccaaaaa
     8821 atatgaccaa tttttaaccc agatgtatgg cgattatatg acaccaccat caaaagaaat
     8881 gcaagagtgg tatagtcata acattaaagc ttatcgcaaa aactgattga gggggattat
     8941 acaaa
//
LOCUS       DAVHIT010000084         8803 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVHIT010000084 DAVHIT010000000
VERSION     DAVHIT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.086"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..389
                     /locus_tag="VVN16_001733"
     CDS             <1..389
                     /locus_tag="VVN16_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5573066.1"
                     /translation="KEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGE
                     YEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQD
                     YGVNLICVEDGIDSSKDAGKLMISVL"
     gene            393..1676
                     /locus_tag="VVN16_001734"
     CDS             393..1676
                     /locus_tag="VVN16_001734"
                     /inference="COORDINATES: protein motif:HMM:NF019149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5573067.1"
                     /translation="MAEIERENIRIQTMEGRIQKAREGKWNGGFAPYGYKLEDGKLFI
                     NEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPLFDAGLIRKILKN
                     PVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDEVWQAAQVKLKSQ
                     AKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKDFYYYGCKHRQ
                     MIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTSEIEKEIDN
                     YQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKIDELESSLIDAK
                     AKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEIQVYEEKQSNGQW
                     LKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            1944..2510
                     /locus_tag="VVN16_001735"
     CDS             1944..2510
                     /locus_tag="VVN16_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573068.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            2832..3020
                     /locus_tag="VVN16_001736"
     CDS             2832..3020
                     /locus_tag="VVN16_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU5573069.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3082..3840
                     /locus_tag="VVN16_001737"
     CDS             3082..3840
                     /locus_tag="VVN16_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5573070.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     ISEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKSYFEKNEIFKIQQDVIF"
     gene            3898..4752
                     /locus_tag="VVN16_001738"
     CDS             3898..4752
                     /locus_tag="VVN16_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5573071.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMCISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            4864..5748
                     /locus_tag="VVN16_001739"
     CDS             4864..5748
                     /locus_tag="VVN16_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU5573072.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            5911..7650
                     /locus_tag="VVN16_001740"
                     /pseudo
     CDS             5911..7650
                     /locus_tag="VVN16_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            7925..8227
                     /locus_tag="VVN16_001741"
     CDS             7925..8227
                     /locus_tag="VVN16_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573073.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            8283..8711
                     /locus_tag="VVN16_001742"
     CDS             8283..8711
                     /locus_tag="VVN16_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573074.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
BASE COUNT         3068 a         1231 c         1879 g         2625 t
ORIGIN      
        1 caaaagaaaa aataaaagta tacctctata cacgagtatc tacgtcaata cagatagagg
       61 ggtattcgtt agaggcacaa aaatcacgaa tgaaagcttt tgctatttac aacgattatg
      121 aaattgttgg agagtatgaa gatgccggga agtctggcaa gtctattgaa ggtagaatac
      181 agttcaatcg aatgatggaa gatataaaat ctggaaaaga tggagtatct tttgttcttg
      241 tgtttaagtt atcaagattt gcaagaaatg ccgctgatgt tctttctaca cttcagataa
      301 tgcaggatta tggagttaat cttatctgcg ttgaagatgg cattgattca tctaaagatg
      361 ctgggaaact aatgatttct gttttataag ctgtggctga aatagaaaga gaaaacatcc
      421 gtattcaaac aatggaaggt cgcattcaaa aagcaaggga aggaaaatgg aatggtggct
      481 ttgctccgta tggatataaa cttgaagacg gcaagctgtt tataaatgag gaagaggcag
      541 ttgcaataag aacgattttc gaccagtatg taaatactac gataggagct aatgggatat
      601 ctaaatactt agagaatcat ggaattagaa aaatcccaag acagaatggt aagaatcctt
      661 tgtttgatgc aggtcttata agaaagatat taaagaatcc tgtatataat gggaaaatag
      721 catttggaag aagaacttta gaaaaagttc atggtacaag aaatgaatat aagcaggttg
      781 aacaagatga atatctaata tctgaaggga tacatgaagc tatagtttcc gatgaagttt
      841 ggcaagctgc tcaggttaag ctaaaatctc aagcaaagaa atatgagcat gtgaataaag
      901 gaaaagatac acgcacacac ttgctttctg gaattgtaaa atgcccgata tgtggagtgg
      961 gaatgtttgg gaacaagtgt atcaagaaaa agaaagatgg cacaaagtat aaagattttt
     1021 attactatgg ttgtaaacat aggcagatga taagaggtca taagtgtaca ttcagtaagc
     1081 aaattagaga agaattgtta gatgatgctg ttgcagaggt gattgtcaag atagtaagta
     1141 atccgaaatt tgcttctatg atgcaagaaa aaatcaacat gaaggtggat acctctgaaa
     1201 tagaaaaaga aatagataat taccaaaagg aattgagaaa gagtcattct acaaagttta
     1261 agctaattga ggaaatagat aatttagatg ttgaagataa gcattataag agaaggaaac
     1321 aggatttaga tgatagacta tatcgtatgt atgacaaaat agatgaatta gaatcatcac
     1381 taattgatgc gaaagcaaag aaacagacta ttgaagctga aaagcttaca ggagataaca
     1441 tatataaggt tctgatctat tttgataagc tctataaagt catgaatgat gtagagcgta
     1501 ggcagttaat ttcagctttg atttctgaaa ttcaagttta tgaagaaaaa caatctaacg
     1561 gacaatggct aaaatccatt acttttaagc taccaattat agaagaaaat ctcaacatag
     1621 gtttggacaa tgatgaacaa gttgaatgtg taagtttatt agagaaacgt agttaatcct
     1681 caaaaggttc cccgaacctt ttgagtccca ctgacgcatc acgctgaggc ggtatcactg
     1741 cttgtacgag ttgaagcatc agcaaagtaa aagtagatgt tcagccaatg cgacgaggtg
     1801 agaccgaagg tcgataaggt gtgttctggg tataggactc gtaaagtaag taggaaatat
     1861 gatgagatga tacagagagc ctgaattggt caggtgacgt gcttatttac attagcctga
     1921 taatgaaaat ggagagatta tcgatggtta tattggaatt gtaccaaaat gattattcta
     1981 aagatttagt agcatttgat tccatagaag atgggaaagc ctttgtagct caaatcccgg
     2041 gatacactct ggaaacagaa gatggttttg aagtggagta ttttaatcct aaaaacatac
     2101 cggattatat ggaaattatt tttaacggaa atattgttcc tttatctaga tttatgttcg
     2161 atcctgggga aaatgtaaac attatttgga aagagatttc aaatttatcc ctcaaaaatg
     2221 atagagtgat cgaaggatat tcaaaaattg atgcatatgt cgttaacaac catgaagtta
     2281 aagcctatgt cgaaacaaga gaaacgaact atcgtaaagc aaaagacttc ttagaaagcc
     2341 gcggatatga aatcgacaga agctttttcg gaagtgaaga cggtgaagcg attctttaca
     2401 gaaaaaaagg gatcgaagtt tggcatttct tgtgtcactt agacccaatg tttgtagaga
     2461 ttgaagatgt agaaggatat gttaaagaag aagttggaga gattcaatag tcaaccaaag
     2521 ttccttgtga gtatatacct ttgctagtaa aagagtcaaa acgttataac gaaggggggg
     2581 aagaaaaccc ttgagtgtaa gtctttttct tgtttagagg gaaagtatta gagtagccgc
     2641 tcataattta tactagattt gataactgat ttgagacaga ttttatttct ataaatattg
     2701 tgaaaatttt ttaataccaa agatttaaag ttactgtgtt aaaaatcctc tccactttta
     2761 aactaatgta ttataaaagc cagttgacac aagtgttaca cctgtgatag aattaacttg
     2821 aggtgatgaa aatggcaacc gataaaaata gaataatgat tagcttggat gataaaaatc
     2881 tagagaaact tgaaaattta gtagaagatg ctagagatag aaggggaatg cgactaacaa
     2941 agtcccaggt tattgaatta ctcctaaata ccgttgatta tttcgatgat attatgggag
     3001 ctatttactc aaaaaaataa gagcaaaatt gctattattt tttacaaatt ggtgtaacac
     3061 tagtgatata ggagtgatga aatggttgtt tatattagac aaagtaaatt accaagtgag
     3121 gtttcaatta ataaatacaa tgctcaagta ggtgcctact tacaaggaga agaagttatt
     3181 ttatatcaat ctttttctga aataaaagag ttaataagtg aagatatagt tgtagattat
     3241 ataatggaga ctagagcatt actaaaaatg atgggcttaa acgttccggt ttatgactat
     3301 cctattgagc ttaaagagtt ttatggtcga aagatttatg ctggtatttt aggagagatt
     3361 gtgaatatac ctgataattg gggaaaattt attaagccta aagctggttc aaaagtcttt
     3421 actggaagag ttgttaatgg aacccatgat ttaataggta ttggtctacc tttcgactat
     3481 cctatatgga ttagtgaggt tgtagaattc atagctgaat ggcgttgttt tgtgttagat
     3541 ggtcgcgtat tagatgttcg accctataca ggtgattatc atgcacaatt tgatgcaagt
     3601 gtaattgatg aagcgatatc atgttggaaa gatgcgccaa tagcttatgg actagatatc
     3661 ggtgttactc gcgatggcag aacacttgtt gttgaagtaa atgatggtta tgcattggga
     3721 aattatggcc tatctccttt aaaatcaatc aattttcata gagctaggtg gaaagaaatg
     3781 gtaaaatcct attttgaaaa aaatgaaatt tttaaaatcc aacaggatgt tattttctaa
     3841 aataaatatg gtagataacc aatataaaaa gcatcaaaat gaacaaggag gaatctgatg
     3901 cttggagcaa ttattggaga tattgtcggt tctgtttacg aatggaacaa tatcaaaaca
     3961 aaggactttc ctttatttcg gaaggactgc ttttttacag atgacacggt tatgacctgt
     4021 gctgttgcag aagcaattat gaatggtgga caaaaagacg atttcattga cgcgatgaag
     4081 aaatatggca gaatgtatcc gaatgctgat tacggtgctc ggtttaatgc atggctaaac
     4141 agcgataacc gtgagcctta taatagcttt gggaatggat cagctatgtg tatttctcca
     4201 tgtgcttgga tcatggactg tggtttttat gcgaaaactg gtatgtggcc atcatctaga
     4261 ggacttacga gtctttctgc agaggtaact cataatcatc cagaaggtgt caaaggcgct
     4321 atggctacag ctgatgctat ctttctatgt cgtttttact ttggtggtta ttgtagagag
     4381 tacgagcaat caattaacga caatcctaca gagtgtaaaa gacgaattaa ggattatata
     4441 gaaaaagaat acgattacaa tctatctcaa actttagatg aaatccgccc taactatcgt
     4501 tttaacgaaa catgtcagga aactgtccct caagccatta tcgcctttct tgaaagtaga
     4561 gacttcgaag atgcgataag aaatgccatc tcacttggtg gcgacagtga tactctcgca
     4621 gcaatcactt gtaacatagc agaggcagct tacggtattc ctgattggat caaggataag
     4681 gcctattctt acttggatga acccttaaag gatgtagtta ggcgatggga aaatagaata
     4741 aaagcgtatt aatctccagt acgtcagatt tctcttttct gtattttcat tgtataatag
     4801 gattacattt acttgcatac gacgaaatgt aaacatttct gtttaaaatt aaggataaaa
     4861 tcaatgaaaa aagcaatggt aattatcaac cctacttctg gtggcgagaa ggctttggat
     4921 tacaaagaaa agctggagaa taaagcaaaa gaatactttg aatatgttga aaccaaaatt
     4981 accgaaaaag cgctggatgc aacacatttt gctgaagaag cttctcgtga gcagtatgat
     5041 gcagtggttg tgtttggtgg agatggaact gtcaatgaag tcatttcagg tattgatgag
     5101 agagactaca ttcctaagtt agggattatc ccaggcggta cgggtaacct cattacaaaa
     5161 cttttggaaa tcaatcaaga catcgatggc gcaattgacg aactggattt tgatttaacc
     5221 aataagattg atatcggtaa agcaaatgac aactattttg gttatatctt tagtatcggt
     5281 tctctgcctg aggcgattca caatgttgaa atagaggaca aaacaaaatt cggtatttta
     5341 acctatgctg taaataccat gaagtctgtc atgacagatc aggtctttaa cattaaggtt
     5401 gagacagaaa atggaaatta tgttggtgaa gctagccatg ttttggtcct tttgacaaat
     5461 tacttcgctg ataagaaaat ctttgaagaa aacaaggacg gctatgccaa cattttgatt
     5521 ctgaaagatg cctctatatt ctccaaatta tccgtcattc ctgatttatt aaaaggggat
     5581 gttatcgcaa atgataatat cgagtatatc aaagcgcgta atattaaaat ctcttcagat
     5641 agtgaattgg agtcagatgt tgacggagat aaatcagata acctacctgt agaaatcaaa
     5701 gtcctagctc agcgagtaga agtattttca aaaccgaaag aggattagta tatagagaaa
     5761 gcctttttta aggctttttg tatactttaa aagatagttc ctttaacaac ggacattcct
     5821 tgcaaatagt ttacaaaaat agtatactgg attcattgag tttgaaaacg tttgcgtaaa
     5881 atttgaatga atactttagg agacaaattg atggaattga gtgctattta ccataggcct
     5941 gagtcggagt atgcctatct ttataaggat aagaaactcc atattcgaat tcgaactaag
     6001 aaaggggaca ttgaaagcat caacttgcac tatggggacc cttttatctt tatggaggag
     6061 ttttatcagg atacaaaaga aatggtcaag ataacttctg gtaccttatt tgaccattgg
     6121 caggttgaag tgtcagttga ctttgcacgt atccagtatc tctttgagct cagagataca
     6181 gaaggtcaaa atattttgta tggcgataaa gggtgtgtgg aaaattctct agaaaatctt
     6241 catgcaattg ggaatggatt taagttgcct tatcttcatg agattgatgc ctgcaaggtt
     6301 cctgactggg tttcaaatac ggtatggtat cagatatttc ctgaaagatt tgccaatggc
     6361 aatgctctat taaacccaga agggacttta gactgggatt catctgtcac acctaagagc
     6421 gatgatttct ttggtggtga tttacagggg attattgatc atatggatta cttgcaagac
     6481 ttgggtatta ctggactata tctttgtccc atctttgaat ctacaagcaa tcacaagtac
     6541 aatacgacag attactttga aattgaccgt cattttggag acaaggagac ctttcgggaa
     6601 ctggtggatc aagcgcatca tcgtggcatg aaagtcatgc tggatgcggt atttaatcat
     6661 attggttcgc aatctcttca atggaaaaat gtcgtcaaaa atggtgaaca gtctgcttat
     6721 aaggattggt tccatattca acaattccca gtgacaactg aaaagctagt taataagaga
     6781 gacttaccct atcatgtttt tggtttcgag gactatatgc ctaagctaaa tacagccaat
     6841 ccagaggtca agaattatct tttaaaggtt gcgacttatt ggattgaaga gtttaatatc
     6901 gatgcttggc gtttggatgt ggctagtgag attgaccatc agttctggaa ggattttcgt
     6961 aaggcagttt tagctaaaaa tcctgatctt tatatcctag gagaagtctg gcatacatct
     7021 cagccttggc taaatggaga tgagttccat gccgtcatga attatccttt atctgatagt
     7081 atcaaggact atttcttacg aggaattaag aagacagacc agttcatcga tgaaatcaat
     7141 tgagagtcta tgtattacaa gcagcagatt tcagaggtca tgtttaatct cttggattca
     7201 catgatacag agcgaatcct gtggacggcc aatgaagatg ttcaactggt taaatcagcc
     7261 ttagcctttc tcttttaaca aaaaggaaca ccgtgcattt attacggaac cgagctagcc
     7321 ttgactggag gaccagatcc agattgtcgt cgttgtatgc cttgggaacg tgtatcaagt
     7381 gacaatgata tgctgaactt tatgaagagg ctgattaaga ttcggaaata cgcgtcagta
     7441 atcatttcgc atggcaagta tagccttcaa gaaatcaaat ctgatctagt agctctggaa
     7501 tggaaatacg aaggatggat cctcaaagca atattcaacc aatcaacaga agattatctt
     7561 ttagagaaag aagcagtagc actagcaagc aattgccaag aattggagaa tcagcttgtc
     7621 atctctccag atggatttgt gattttctaa aaactagttg atgaagatta tggtacattt
     7681 catatcttat atagtataat aaggctagtt actaaacttg taaaggagaa cttaaatgaa
     7741 ttgtagagga catgaaacaa gacaaagaat tgttagagat tttgaagttt agcctaaagc
     7801 acatattaag ctgttagcaa atcaacaaaa acatagtgat gcaggagcaa ctattgaaga
     7861 tgaatattat gtatttatcg ctgagagtaa aattgatggc aagaaggaag ttattcagtg
     7921 ttgcatgggt gcggcaaggg attttttaga actaattaat cacaaagggc tacctctttt
     7981 taatccgctt gtaggtgatt ctcatgtaaa taatagacaa gaatatgaca atacagggag
     8041 tggaaattta caacctgaaa agtggaatga aactgcaaag cagctttata atgttataat
     8101 gtggttgatt attttatgga atgctaagcc ggatacacct ttatttaatt ttaaagacga
     8161 agtaattaag tataaaacat atgagccttt tgaaagcagt ataaaattgg tatcaatcaa
     8221 tgagtaacaa agtatcagca tgattcgaaa catttacttg taatttgcgg aggaacatca
     8281 atatggatgg aataacaaaa gattttataa aaacggctaa actaatgaaa caattatggc
     8341 cccaattgac cgataaagaa gctattgatg aagtaaaaaa atatacgaat ggcaaaaata
     8401 ctgcaatctt tactgaagtt gaaggtgaca caattgtagg tctagcacta tgttcactca
     8461 gatttgatta tgttgaaggt tgtaaatata gtcctgttgg attcttagaa gggattattg
     8521 ttgacgagga atatcgttta aaggatattg ctaaaaatct ctgtacaaaa tgtgaggaat
     8581 gggcgaaaaa taaaggatgt aaggaatttg caagtgactg tactttaacg aatacggatt
     8641 ctataagatt tcatctcaat attggatttc aggaggcaaa tagaattatt cattttaaga
     8701 aaaaattata atttagaaac gtgaataaac ttgtttaaac tttatgtatc tttagaaagt
     8761 gtgttaactt gaaagaacaa atttttttga tgggtgggaa tcc
//
LOCUS       DAVHIT010000085         8513 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVHIT010000085 DAVHIT010000000
VERSION     DAVHIT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.087"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..174
                     /locus_tag="VVN16_001743"
                     /pseudo
     CDS             1..174
                     /locus_tag="VVN16_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(302..505)
                     /locus_tag="VVN16_001744"
     CDS             complement(302..505)
                     /locus_tag="VVN16_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573075.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(498..1580)
                     /locus_tag="VVN16_001745"
     CDS             complement(498..1580)
                     /locus_tag="VVN16_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU5573076.1"
                     /translation="MSKLQQILIYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFENAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1706..2488)
                     /locus_tag="VVN16_001746"
     CDS             complement(1706..2488)
                     /locus_tag="VVN16_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU5573077.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2488..3831)
                     /locus_tag="VVN16_001747"
     CDS             complement(2488..3831)
                     /locus_tag="VVN16_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5573078.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3930..5246)
                     /locus_tag="VVN16_001748"
     CDS             complement(3930..5246)
                     /locus_tag="VVN16_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU5573079.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERNKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQNLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5439..6665
                     /locus_tag="VVN16_001749"
     CDS             5439..6665
                     /locus_tag="VVN16_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU5573080.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6864..8438
                     /locus_tag="VVN16_001750"
     CDS             6864..8438
                     /locus_tag="VVN16_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5573081.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LNEKQTGFVIRNKGDK"
BASE COUNT         2435 a         1638 c         1888 g         2552 t
ORIGIN      
        1 gatggagtcc ttctttggga ttctgaaatt tatggctatg agaaaacatt taaatcactt
       61 aatcaattgg aacaagctat tgtagactat attgattact acaacaataa acgaattaag
      121 gtaaaactaa aaggacttag tcctgtgcag tacagaacta aatcctttgg ataaattaat
      181 tgtctaactt ttggggggca gtacatttta acacttgata atatgcgttt tattgtggga
      241 agattttcct cattttctag tgaaattgaa ctttttctaa catcttttga tagatgactt
      301 cttaatcaat tatcatttcc attgttgttt cttggggttt catcccttgt ctttgataaa
      361 agcggagagc accctcgtta tcattccaga cacttagggt tagattattg cagccaattt
      421 ctttagcata atagcagaaa aatgacaata aaatagcccc tagatatttt gaaaggaaag
      481 cataggggct atatagttta atcaaaataa agcaaatctt tgctggtgct tgtaaagagg
      541 tcgaagccat ccttggtaac aacaccgcag tcttcaatac gaacaccgac tttaccaggg
      601 atatagatac ctggttcaac agagaagcac atgccttctt cgatgaccat gtcgtttcct
      661 tccatgatag atgggaattc atggacatcc ataccgatac catgcccgag acggtggttg
      721 aagtactcac cataaccagc tttttcgatg acctcacggg cagcgcggtc cacttcatga
      781 gcagtcacac ctggcttgat aaagtcaaga gcagcttgtt gggcttcaag agtcaagttg
      841 taaatatctt tcttgaattg gtctggtttg ccgacagcga ctgtacgagt catatctgac
      901 gcatagccat tgaccagaac acccaggtca aagaggagaa gagcatcatt ttcaacctta
      961 ttagctgctg gaatgccgtg tggattcgca gcattatcac cagtcaagac catggtatca
     1021 aagctcattt cgtaaccttc acgtttcatg gcaaagtcaa tttgtgcgat gatatctgtc
     1081 tcagtcttat caagagaaat attgtcaaaa ccaacatgaa cagccttgtc agcataaaga
     1141 cctgcaacca tcattttttg cacttcatca gctgatttga tgaggcgcat gcgttggata
     1201 cgaggagtga ggttttcaaa ctcagcattt tcaaaaactg ttttcaaacc atggtatttg
     1261 gtcaagatga gattgtcaaa ctcaacagcg acacgtttga agtcaagttg tggaagagca
     1321 tgtttgattt tttgccatgg attttcagaa tcgacatagc ccactactgg gaaggaaacg
     1381 gtgctacttg cacgttctac ttcaagagct gggacaaaga ggagaggttc ctgatccgct
     1441 aggacaaaga ggaacatttg gcgttcatgg ggatcactgt aaaaaccagt gaggtaattg
     1501 attgtgacgg ggtcagatac gacagcgacg tctagttttt ctgattcaag atatattagg
     1561 atttgttgta atttagacat atgctacctt ctttctaccc ctctattttg gcaaaaaatg
     1621 agagaaaatg caagggagaa gggtaaaaga aaagacaaaa aactccgaca agataacgga
     1681 gttctttgat atcattttcc tttgtttaag aaaagtcagc cttgcttttg acgtcactgt
     1741 actcttcagc gatttcttgg ctgagaatgt cctcataggc agggagttgg atgtcctgac
     1801 catatttctt cttgagggca gtagtaacta ggcgataagc cagattggca ttacgagaga
     1861 ggataggccc gtggaagtag gaaccaaaga cattcttata atgaacccct tcaccgacct
     1921 tttcttcgtt gtttccattt ccatagacaa cctgtcccag cggtttttgg tcatcagaga
     1981 ggaaggtacg accttggtga ttttcaaatc catagtaggt ttcatcgaaa tcttcattgt
     2041 gaatcttgat gtcaccgata aaacggttat tggtctggtt gagcgtgtag tgtcccatga
     2101 cccctagccc ttcgatacgt tttcctgaag cttcaacata atattgaccc aatagttgga
     2161 aaccaccgca gatagccaga actacaccgt cgttttggat gtagttgtca atgctctctt
     2221 ttttagcagg taggtcgtct gcaatgatac tttgttcaaa gtcttgacca ccaccgaaaa
     2281 aggctatgtc gtagtgattt tcatcaaagt catcatggag agaaacgatg tcaacggtca
     2341 catgggctcc cagtttttca gccacatact tgagcatgag gatgtttcca ttgtccccgt
     2401 aggtattcat gagatttccg tagaggtggg caatgttgag ctgatagggg taattgccat
     2461 cttttgagga aagtgaagta taaaccatta gttcatctcc tttctaacaa tctgacgact
     2521 agccagcagt tcacgaaatt ccagcatggc agtataagtt gccagaatat aggcatgctt
     2581 gcagtcttga ttctcaatgg tcttgagaac ttgctccaga ttactcgttt cagtgatttt
     2641 ctcagctgga tagccagtca ctcggaggcg acgagcgatt tcagaatgac gaacaccgcc
     2701 agcgttgatt tcaggaatgt ccatgtcagt gatttgctca aagtctgcat cccagatcca
     2761 gctagtgtca attccatctg catagttggc attaaggagg acagataggc taaatggata
     2821 aggtgctagt ttgatcattt cgatagcttg ggttgcaccg actggatttt taatcaagac
     2881 aagggtacat tccttgtcac cgatatgaaa ggtttcttgg cgtccaaaga cagcacggct
     2941 cttgtcaaat ccctgtttga tgagttgcga atcggcacct aggaaacggg cgatggccac
     3001 agcagctagg gcgttataga tattatagag cccgccaatt tggataccgt attcttggcc
     3061 gtctatgaca aagcgagagc gattgttggt caactcaacc agttttgtca aacgatagtc
     3121 gagatcagga cgtttgcatc cacaaccttc acagatatag gcacccaagt ttgcataggt
     3181 attatgctca tatttgagga tgccttggca gtcaggacag agaatccctt cggtattgta
     3241 gtgagccagt tgggctggtc ctttttccaa gtcaaaacca aaatactcta tagggtttgg
     3301 aatagttggc ttgtagaaaa gtggactgtc tccgttaagg agaacagtag cagttggaac
     3361 tttccgaatg gcttccaata tcatgttata ggtagtatag atttcaccga aacggtccat
     3421 ctggtcacgg aagatattag taatgacaaa aagactaggc tggatatagt cacagatacg
     3481 agatagactg gcttcgtcaa tttcgaggac ggcaatattt ttcccagttt ttgaagattt
     3541 ggctgttagg aaggttgttg caatccctgt aatcatgttg gcaccgcttg ggttggttag
     3601 aacttgacca taaacctctt ttaaaatgcc gacagtgagg gcagttgtca gggtttttcc
     3661 atttgttcca gtgacaacga caatctcgta gttcttagct aggttttgta aaatatcttt
     3721 atcaaattga agggcgactt tacctgggag cgtacttcca cggccaagac ggcttaaaat
     3781 gaagtgggaa gaacgcccag caagaaggcc caaagtagtt tttaagttca tgtttctatt
     3841 atatcataaa agaggagaaa gacagatttg agattccgca gaagtaaaaa cagcccaaag
     3901 agggctgttc gatacgatta aatctcaact taaatcgcaa acaagtcatt caagttctca
     3961 gccaaggttg ggtgagtgaa gatttgtttt gtgaagtaag tgtaaggaat cttgttgtcc
     4021 atagcaacag tgatgatgtt gatgatttct tgagaacctt ctgagaagat gcttgctcca
     4081 agaatttctt ttgtttcagt attgacaaca gctttgaagg caccgcgaag gtctccattt
     4141 acgtgaccac gaggcattgc tgcaacgggg atttccttaa cagcgtatgg aagtttcaaa
     4201 tcagctgctt ggctttcagt caaaccaact tgtgaaagtg caggtgtgat gaacatagta
     4261 tttggcacat tgagacggtc ttcaagtgtg tagctgccat ctccagcaag gtagctgtaa
     4321 acaacacgga agtcatcaag tgaaatgtaa gtaaattgaa ggccaccgtt gacatctcca
     4381 actgcaaaga caccaggaac gtttgtttga cagtgtttgt ctactttaat agcaccacgt
     4441 tcagttagtt caatatctgt attttcaagt tgaagtggtt ctacatttgg tttgcgtcca
     4501 gttgcgtaga gaagggcgtc gaaacggtaa gtttcgtctt cagttacgac aagcacttgg
     4561 tcaccatcgt ttttgatttc agtagtatgg atattttgaa gcaattcaat gccatcttct
     4621 tccatgtatt gtttagcaag agctgcgatg gaaggttctg cacgaggtag gaatgtatcc
     4681 aaggcatcta ggactgtgac cttgcttcca agtttgttgt aaaggccggc aaattcaaga
     4741 ccgatatttc cgccaccaag gattccaagt ttttcaggta atttgtccaa gttttggata
     4801 cctgttgagt caaagatgtt tttgcttgta gcaagtccag ggattggcaa gacgtttgaa
     4861 acagcaccag tgttgatgac gattgtttca gcagtcagtt ctttcttttc atcaccagct
     4921 tggatttcga tgactttatt tgaaaggaag tgagcttccg catcaaagat atctacgcct
     4981 gtaccagcaa cagtcgcata gtttttaccg ttgaggcgac cagtgatcgt gtttttagta
     5041 gcaatgactt cttcaaaaga caagtccttt tcagcagcaa ctagcaaggt tttagttggg
     5101 atacaaccaa tgttgataca agttccaccg tacatagcct tgttgcgttc aacgagggca
     5161 acttttttgc cagctgaagc caatttacct gctagtgttt taccagcttt accaaatcca
     5221 ataacaatta aatcgtatgt taacatttat cttcctccta ttcgaatttc attctagcac
     5281 aagaaaaaaa cttttgcaca attctgctac ggaaaagaca gaaaaagata aaaacgcttt
     5341 ccagacaaaa aactagtaat tttttgacaa gcttgataaa ctaaaatcct atctattttt
     5401 atataagata gaataaatgt tcgaaaatag gaggttttat gaaatcgaat cgttatatta
     5461 ttgcctttgc tggggtcatt ttacacttaa tgctgggttc gacttatgct tggagtgttt
     5521 atcgtaaccc tattattgaa aaaacgggat gggatcaggc ttctgttgcc ttcgccttta
     5581 gtctagcaat cttttgtttg ggtttatcgg ctgcatttat ggggcgtttg gtagaaaaat
     5641 ttggtccgaa agtcatgggg agtctatctg cttttctata cgcaggtgga aatatcttaa
     5701 caggatttgc aatagaccgt caggaactgt ggttgttgta tctagcttat ggcattttag
     5761 gtgggcttgg tttgggagca ggctatatta cccctgtgtc aacgattata aaatggtttc
     5821 ctgataaacg tggtctcgca acaggtttag cgattatggg gtttggtttt gcttctttat
     5881 tgactagtcc catagcgcaa caccttatcg caggggtagg gattgtagaa actttttata
     5941 ttttaggagc aagttacttt attatcatgc tcctagcttc acaattcatt aagcgtccaa
     6001 atgagcaaga gcttgcaatt ttatcttctt cagggaaaga aaaaacagcc tctttgacgc
     6061 aaggaatggc tgcaaatcag gctctaaaaa gcaatcggtt ttatatgctt tggattattt
     6121 tctttatcaa catagcttgt ggtttaggct taatttcagc ggcatcgcca atggcacagg
     6181 agatggctgg cttgtctaca agtcatgcag cagtaatggt gggtgttttg gggattttca
     6241 atggatttgg tcgcttgctc tgggcgagtt tgtctgacta tatcggtcgc cctctaacct
     6301 ttagtatatt actgcttgtc aatcttttct tttctctctc actttggctc tttacagatt
     6361 ccgttttatt tgtagttgct atgtctattt tgatgacttg ctatggagct ggtttttctt
     6421 tgattccagc ttatctcagt gatatttttg gaaccaagga attggccgct ctgcatggtt
     6481 atattttaac agcttgggca atggctggtt tagcgggacc tattttatta gcagagactt
     6541 ataaaatggc tcattcgtac acacaaacct tgttcgtttt tctcatttta tacagtatcg
     6601 ccttggcttt gtcttattat ctaggtcgtt caatcaaaaa agaaagtcaa aaaccgctta
     6661 catgattttc tgtagaaatg gggctatctt ttgctctggt cttgtgttat actagatagg
     6721 ttgcaaagaa aacagtactt ttcttttgtg gaaaagaagc aacacgattt tatatctagt
     6781 tatatgaaaa tacgtcataa aaagaaaagt ataaactaag ccctataggg tggcttcgca
     6841 ccacctttag aaagaagaat aacgtgaaat ttaatgaatt aaacttgtct gctgatttgc
     6901 tagcagaaat tgaaaaagct ggttttgtag aagctagtcc tatccaagaa caaactattc
     6961 ctttggccct tgaaggcaag gatgttatcg gtcaagctca gactggtaca ggaaaaactg
     7021 cagcctttgg cttgcctacc cttgaaaaaa tccgtacaga agaagcgact atccaagctt
     7081 tggtcatcgc tccaactcgt gaactagctg tccaaagtca agaagaactc ttccgctttg
     7141 gtcgtagtaa gggagtcaaa gtccgttcag tatatggcgg atcaagcatt gaaaaacaaa
     7201 ttaaggctct taaatctggt gcccatatcg tggtgggaac tccaggtcgc ctcttggact
     7261 tgattaaacg caaggccttg aaattacaag acattgaaac ccttatcctt gacgaagcgg
     7321 atgaaatgct taacatgggc ttccttgaag acatcgaagc cattatttcc cgtgtacctg
     7381 agaaccgtca aactttgctt ttctcagcaa ctatgccaga tgccatcaaa cgtatcggtg
     7441 ttcagtttat gaaagcccct gaacatgtca agattgcggc taaggaattg acaacagaat
     7501 tggttgacca gtactatatc cgtgttaagg aacaagaaaa atttgacacc atgactcgtc
     7561 tcatggatgt ggcacaacca gaactcgcta ttgtatttgg tcgtaccaaa cgccgtgtgg
     7621 atgaattgac tcgtggtttg aaaattcgtg gcttccgtgc agaaggaatt catggcgacc
     7681 tagaccaaaa caaacgtctt cgtgtccttc gtgactttaa aaatggcaat cttgatgttt
     7741 tggttgcgac agacgttgca gcgcgtggtt tggatatttc aggtgtgacc catgtctaca
     7801 actacgatat tccacaagat cctgagagtt atgttcaccg tatcggtcgt acaggtcgtg
     7861 ctggtaagtc aggtcaatct attacttttg ttgctccaaa cgaaatgggt taccttcaaa
     7921 tcattgaaaa cttgactaag aaacgcatga aaggtctcaa acctgcaagt gtagaagaat
     7981 ccttccaatc aaaaaaacag gtagctctca agaaaatcga acgtgatttt gcagatgaaa
     8041 ccattcgtgc caactttgag aaatttggta aggatgctcg caaattggct gctgagttta
     8101 ctccagaaga attggcaatg tatatcttga gtctgacagt ccaagaccca gatagccttc
     8161 cagaagtgga gattgcacgt gaaaaaccac taccgtttaa accatcaggt aatggtttcg
     8221 gtggtaaagc taagggaggt cgtggaggcc gtcgtgggga tgaccgtcga gagcgtgatc
     8281 gccgtggcaa tggtcgccgt gatgagttca aaaaaggaag tcgtggcaac gatcgttttg
     8341 ataaggaaaa acgttaccgt aaggataata aaaaaccacg caatacttta aacgaaaagc
     8401 aaacaggctt tgttattcgt aacaaaggtg ataaataaga aaaagcggtt caaaatgaat
     8461 cgctttttta tataaggaga ccgaatgtga aaaagacaaa taaaattatt ata
//
LOCUS       DAVHIT010000086         8150 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVHIT010000086 DAVHIT010000000
VERSION     DAVHIT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.088"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..120
                     /locus_tag="VVN16_001751"
     CDS             <1..120
                     /locus_tag="VVN16_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU5573082.1"
                     /translation="GNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            133..1608
                     /gene="galT"
                     /locus_tag="VVN16_001752"
     CDS             133..1608
                     /gene="galT"
                     /locus_tag="VVN16_001752"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU5573083.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMSEDIPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKTELEEVGKYLLGEDNAIAAYHLEWADQLKEKYSGINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1621..2631
                     /gene="galE"
                     /locus_tag="VVN16_001753"
     CDS             1621..2631
                     /gene="galE"
                     /locus_tag="VVN16_001753"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5573084.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVVIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2798..3457
                     /locus_tag="VVN16_001754"
     CDS             2798..3457
                     /locus_tag="VVN16_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU5573085.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3482..4549
                     /locus_tag="VVN16_001755"
     CDS             3482..4549
                     /locus_tag="VVN16_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5573086.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALTELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            4652..5662
                     /locus_tag="VVN16_001756"
     CDS             4652..5662
                     /locus_tag="VVN16_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573087.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5674..7365
                     /locus_tag="VVN16_001757"
     CDS             5674..7365
                     /locus_tag="VVN16_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5573088.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7368..8078
                     /locus_tag="VVN16_001758"
     CDS             7368..8078
                     /locus_tag="VVN16_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5573089.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2739 a         1130 c         1607 g         2674 t
ORIGIN      
        1 gggaacgttt ctgaagtaat tggtgctttg gatattattc atcaccttga taaaattgct
       61 catactacgc aatcaattta taaatggata atgtatagaa aatatgggaa cataaattag
      121 aaagaaatat atatgaaaac aattattgat aattttgttg atagagtaat tgaatacgga
      181 atgtataatg aaattgacaa aatatatgtg aaaaatagag tactagcgtt aataggtgag
      241 gagggaactg atcgcatttc cgatgaaaat gacctgaaac aaataaaaga ttacttagtg
      301 gaaatcgcct taaaaaacgg taaaattaaa gatttaatcg aggaaaagga atgtttaggg
      361 gcagagttaa tgaatttcat agttccttta cctagtcgat taaatgatat tttttggagt
      421 tcatatgata tatcacctca agaagcagtt gaagagtttt ataagttaag taaagatagt
      481 gattatataa aaacttctgc tattgctaag aatattgagt ttcgagcatc aactaaatac
      541 ggagagctag agattacgat aaatttgtca aaacccgaaa aagatccaaa aacaattgct
      601 gctgaaaaat tagtaaaagc aactaattat cccaaatgtt tactatgtat ggaaaatgaa
      661 ggatatcagg ggcgaataaa ttatccagca cgttcgaatc atagaattat tcggttgaaa
      721 cttggtgacg aagtatgggg attccagtat tctccctact catactttaa tgagcatgcg
      781 atatttttga atagtcaaca tgtaccaatg gctattacct ctaaaacatt tgaacaatta
      841 ttggagattg ttgatatttt gccaggatat tttgctggtt caaattctga tcttcctatc
      901 tcaggaggct ccattcttag tcataaccac taccagggag ggaaacatat tttcccaatg
      961 gaaaaagcaa aatttgagag tgaatttcgt tttaaagact ttgaggatgt taatgctggt
     1021 attgtaaaat ggccaatgtc agtaattagg ttacaaagtg aaaataaaaa tcgcttgtta
     1081 gatttagcta ctaaaattct aaataagtgg agagaatatt cagatttaga agtagacatc
     1141 attgctatgt ctgaagatat tccacaccat acggtgactc ctattgctcg taaagtagat
     1201 gggagatatg aattggatat tgttctgaga gataatcata ctactgaaca atacccagat
     1261 ggtgttttcc atcctcatca agatgtacaa catattaaaa aggaaaatat tgggttaatc
     1321 gaagtgatgg gacttgcgat attaccacct cgtttgaaaa cagagttaga agaagttgga
     1381 aaatatcttt taggagaaga taatgcaatt gcggcttatc atttagaatg ggccgaccaa
     1441 ttgaaagaaa aatattctgg tattaacaaa gaagaggtta atagcgtagt tcaacacgaa
     1501 gcaggacaag tgtttgcgag agttcttgaa gatgcaggtg tttataagaa tactccgtct
     1561 ggacatgaag cgtttatgag atttgttaaa tcagttggaa ttaattagga ggaaacccaa
     1621 atggcaatat tggtaacagg cggtgctggt tatattggta gccataccgt agtagaatta
     1681 ctaaatttag gaaaggaagt cgtcattgtt gacaaccttt ctaactctag catcttggtg
     1741 ttagaccgta ttgaagcaat tacaggaata cgtcccgtgt tttacgaatt agatgtttgt
     1801 gataaacaag cattgagaaa ggtatttgaa caagaatcga ttgatgctgc aattcatttt
     1861 gcaggttata aagctgtcgg agaatccgtg caaaagcctg tgatgtacta caaaaataat
     1921 attatgagta cattggcact tgttgaagtg atgtcagaat ttaatgttaa aaagattgtc
     1981 ttttcttcaa gtgcgactgt atatggaatt aacaatcagt cacctctaat tgagacgatg
     2041 caaacaagtg cgacaaatcc ttatgggtat acgaaagtga tgcttgagca aattttaaaa
     2101 gatgttcatg tggcagattc agaatggagt attgcgttgc ttcgttattt caatccaatt
     2161 ggtgctcatg agtctggttt gattggggaa gatccctcag gaattcctaa caacttgatg
     2221 ccctatattg cgcaagtagc ggtaggtaag ctgtcagagc taagtgtgtt cggaaatgat
     2281 tatgatacgc ttgatggtac tggtgtgcgt gattatatcc atgtagtaga tttagcaata
     2341 ggacatataa aagctttaga aaaagtatct gaaaaaacag atgtttatat ctataaccta
     2401 ggctcgggag aaggcactag tgtattacaa cttgtaaata catttgaaag tgttaataag
     2461 atccctattc cttataaaat agttccaaga cgttcaggag acgttgcaac ttgttatgca
     2521 aatgcagaca aagcatataa ggaattaaat tggaggacaa caaaatcgat tgaagacatg
     2581 tgtagagata catggaattg gcaatcaaaa aatcccaatg gctataattg atgatactgt
     2641 ctatatgagt gttgtatttt gggaaaatta cttaaccttt taatgaaaga taatttttta
     2701 aaaaaatgtt tgaaagcgct tgacaaagta aacggtttcg ttatataata taagcataga
     2761 tgacaccggt gtcaaatata aaaaagaggg tctttatatg aaaaacctaa caaaaattaa
     2821 gttcaaagaa aatggagaat ttaatcattt tccaggaaat actgttgtag caaatcttta
     2881 tactaaacaa gatttgatgg aagttgttga tattattcaa tcacgttata gagaattacc
     2941 atttatcgat aagtttactt taactccaag gaattctatt cacatgactg taattgaatt
     3001 gttgtgccat gaaaatcgtg aaacggagtt ttggagcagt aatcttcctc ttgatacacc
     3061 tttacaggaa atacatgatt actttgctaa acaacttgaa atttttccat tgttggatga
     3121 agaaattcat atgcgtgtga ctgaaatggg aaaacaaaac atactagttg aacctgcaga
     3181 tgaagcttct gcaaagagat tagaagaaat tcgtacttat gtttcagaaa aagcaggtgt
     3241 tcgtttccct aatcatgata gataccaatt ccatatttca attggatatc ttcggattcc
     3301 tctaaccgaa gaggaggaag aagagtttac taaagtcaga gcagaattaa ctgaaatttt
     3361 attagagaag attccaacta ttactgtaaa ccgtattgac tatactgtat ttgaagatat
     3421 gagacaattt gttccatatc acgaaaaatt taaataaaat aataaaaaag gagaacttat
     3481 catgaaaaaa atgaaagttt ggtctactgt acttgcaacg ggagttgctc ttactacact
     3541 tgctgcttgc tctggaggtt caaattctac gactgcttct tcatctgaag aaaaagctga
     3601 taaaagtcaa gaattagtaa tctattcgaa ctcagtctca aatggtcgtg gtgattggtt
     3661 aactgctaaa gcaaaagaag ctggttttaa tatcaaaatg gttgatatcc ctggcgctca
     3721 attagcagac cgtgttattg ctgagaagaa taatgcagtt gcagatatgg tatttggaat
     3781 tggtgctgtt gattcaaata aaattagaga tcaaaaatta ctagtacagt acaagcctaa
     3841 atggttagat aaaattgatc aatctttatc agataaagat aattattata atcctgtgat
     3901 tgttcaacca ttagttttaa ttggggcgcc tgatgtaaaa gaaatgccta aagattggac
     3961 agaattaggt agtaagtata aaggtaaata ttcaatttct ggtcttcaag gtggtacagg
     4021 acgtgcaatt ctagcaagta tcttagttcg ataccttgat gataaaggtg aattaggtgt
     4081 ttccgaaaaa ggttgggaag tagcaaaaga atatttgaaa aatgcataca ctcttcaaaa
     4141 gggagaaagt tcaattgtta agatgttaga caaagaagat ccaatacaat atggaatgat
     4201 gtggggttct ggtgcattag ttggacaaaa agaacaaaat gttgttttca aagttatgac
     4261 tcctgagatt ggtgtaccat ttgtaactga acaaactatg gttttaagca ctagtaaaaa
     4321 acaagcgtta gctaaagaat ttattgattg gtttggtcaa tcagaaattc aagtagaata
     4381 tagtaagaac tttggatcta ttcctgcaaa taaagatgcc ctaacagagt tacctgaaga
     4441 tacgaaaaaa tttgttgatc aagtgaaacc acaaaatatt gactgggaag ctgttggaaa
     4501 acatttggat gaatgggtag aaaaagctga attagaatac gtacaataag aaataagagt
     4561 taatttttat gtccaagtct ctctattatt tctaaattta tagaagaaat aaagagaggc
     4621 ttttggttta gtaatagagg gagttagtga gatgattaaa tttgataata ttcaaattaa
     4681 atatggtgat tttgttgcaa ttgataatct gaatttagat atacatgaag gggaattctt
     4741 tacatttctt gggccttcag gatgtggtaa atcaactact ttgagagcat tggtaggttt
     4801 tttagatcca tcatcaggaa gtattgaagt taatggaaca gatgtcactc atttggaacc
     4861 tgaaaagcgt gggattggta ttgtatttca atcttatgcg ctatttccaa ctatgactgt
     4921 ttttgataat attgcatttg gtttaaaagt taagaaggta gctccagatg ttattaaagc
     4981 taaagtatca gcagtggcag caaaaattaa gatctctgat caacagttac agcgtaatgt
     5041 atcagaatta tctgggggtc aacaacaaag ggtagcattg gctcgtgctc tggttcttga
     5101 acctaaaatt ctttgtctag atgaaccatt gtcaaacctt gacgcaaaat tacgtgtaga
     5161 tttgagaaaa gagttgaaaa gacttcaaaa agagttaggt attactactt tatatgttac
     5221 tcatgatcaa gaggaagcct tgactttatc tgatagaatt gcagtcttta acaatggata
     5281 catcgaacag gtcggtacac cagtagagat ttatcataat tctcaaactg aatttgtatg
     5341 tgattttatt ggagatatta atgttttgac cgatgaaaca gtccacgaag tattattgaa
     5401 aaatacaagc gttttcttag aggataaaaa aggatacatt cgattagaga aagttcgatt
     5461 caatcgtgaa actgaacaag attttattct aaaagggaca attattgatg ttgagttttc
     5521 tggagttaca attcactata caataaaagt ttctgaaagt cagattctta atgtaacaag
     5581 tattgatagt caggctgcta ttagatctgt cggagaaagt gtggaattat ttatcacacc
     5641 atcagacgtt ctgcaatttt aaggaggtgc agtatgcgtc ataaattaaa tttaaaagat
     5701 tggcttattc gtttagggtt aatctggttc ttagtaacat ttattattta tccaaacttt
     5761 gatctagtag tgaatgtatt tgtaaaagga ggagaatttt cccttgatgc tgtacatcgt
     5821 gttctaaaat ctcagagggc acttcagagt attatgaaca gttttaagtt agcattttca
     5881 ctcattatta cagttaatgt cgtaggtatt ctttgtgttc tatttacaga gtactttgat
     5941 attaaaggtg ctaaaatttt aaaattaggt tatatgacct ctttaattta tggaggagtg
     6001 gttttagcga ctggatataa atttgtctat ggtccttatg gattgattac aaaattttta
     6061 caaaatgtta tcccttcttt agactctaac tggtttattg ggtatggtgc agtcttattc
     6121 attatgacat tttcaggaac tgctaatcat acattgtttt taacaaatac aattcgaagc
     6181 gttgactatc acactattga ggctgctcga aatatgggag caaaaccatt tactgttttc
     6241 cgaaaagtag tgttaccaac cttaattcca actctatttg cacttactat tatggttttt
     6301 cttagtggtt tatctgcagt agcagcaccc atgattgttg gtggtaaaga atttcaaact
     6361 ataaatccaa tgattattac atttgcaggg atggggaatt ctcgtgattt agctgcctta
     6421 cttgcaatta ttttaggtat tgcaactaca attttgctta ctatcatgaa taagatagaa
     6481 aaaggtggaa attatatttc tatctctaag actaaagcgc ctcttaaaaa acaaaaaatt
     6541 gcgtctaagc cttggaatat cattgctcac attgtagcat atggattgtt cacagttttc
     6601 atgcttccac taatttttat agtattatac tcatttacag atccagttgc aattcaaaca
     6661 ggtaacttaa cattatcaaa ctttacttta gaaaattatc gcttattctt tagtaatagt
     6721 gcggcattct ctccattctt ggtcagcttt atttattcta ttattgctgc gacaacagca
     6781 acaattctcg cagttgtatt tgctcgtgtt gtcagaaaac ataaatctcg ttttgatttc
     6841 ttatttgaat atggtgctct acttccttgg ttactaccaa gtacactttt agcagtaagt
     6901 ttattattta cttttaatca gccacaattt cttgttttga atcagatttt ggtaggtagt
     6961 ttggtaattc tacttattgc atatatagtt gtaaaaatcc cattttctta tagaatggta
     7021 cgtgctattt tatttagtgt tgatgatgag atggaagatg cagcaagaag tatgggtgct
     7081 tcaccttttt atactatgat gaaggttatc attccattta ttttaccggt tgttctctct
     7141 gttattgctt taaactttaa ctctttatta actgacttcg acttatctgt attcctttac
     7201 catcccctag ctcaaccatt aggtattacg attcgatctg caggtgatga aacagcaaca
     7261 tctaatgcac aagctctggt atttgtttat acaattgttc tgatgattat ttctggaacg
     7321 gtattatact tcacacaaag accggggcgt aaagtaagga aataatcatg acagccacta
     7381 gtcttgggtt atcaaatatt gaaatagttg tcaggattgt tttatcagta gtcattggta
     7441 gtataattgg tttagagaga gggagcaaat cccagcctgc aggcatccga acttatagta
     7501 ttgtttgtct agctgcatgt ttgattatga tgacgaatga atacgtatct tataaatttg
     7561 ggacaggaga tcctacacga ttaggagctc aagttatatc aggtgtgggt tttctaggcg
     7621 ctggaacgat tcttattaca gataagaaga aaattacagg tctgacaact gcagcaggca
     7681 tttgggcttc ggcaggaatt ggattagcta ttggagtagg tttttatgag ggagctcttt
     7741 tagtagccat ttctgtttgg ggtgtgatat ccatgttcca accactaaaa aaatatctgc
     7801 aaaatcgttc taaaatgatt gaattgtata tagtagttaa atctacagaa gcctataatc
     7861 gtgtattggt ttattgtgca gaaaatggta tcagaatgac cgattcaaga acagcgtttg
     7921 gagatgttaa ttctgacaga attgaatact ttgatgttcc ggacaaaaaa atagcgtcat
     7981 ttattactct ggaattatca ggtagatttg aacatcttcg actgatggaa gaaattgcta
     8041 atattgtagg tgttatttat gtcgaagaaa ttagctgaaa tttagtaaac aaagggacag
     8101 atgtttttgt ccttttgttt tttagatgtt ttaatcatct aatttttgag
//
LOCUS       DAVHIT010000087         8126 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVHIT010000087 DAVHIT010000000
VERSION     DAVHIT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.089"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(109..183)
                     /locus_tag="VVN16_001759"
                     /pseudo
     CDS             complement(109..183)
                     /locus_tag="VVN16_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            214..447
                     /locus_tag="VVN16_001760"
     CDS             214..447
                     /locus_tag="VVN16_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU5573090.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            447..1781
                     /locus_tag="VVN16_001761"
     CDS             447..1781
                     /locus_tag="VVN16_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU5573091.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1791..2045
                     /locus_tag="VVN16_001762"
     CDS             1791..2045
                     /locus_tag="VVN16_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU5573092.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            2141..2536
                     /locus_tag="VVN16_001763"
     CDS             2141..2536
                     /locus_tag="VVN16_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU5573093.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            2949..3398
                     /locus_tag="VVN16_001764"
     CDS             2949..3398
                     /locus_tag="VVN16_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU5573094.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3385..3945
                     /locus_tag="VVN16_001765"
     CDS             3385..3945
                     /locus_tag="VVN16_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573095.1"
                     /translation="MARTELPDKIETECLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYAFLKSEWEVI"
     gene            3945..4523
                     /locus_tag="VVN16_001766"
     CDS             3945..4523
                     /locus_tag="VVN16_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5573096.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            4516..7167
                     /locus_tag="VVN16_001767"
     CDS             4516..7167
                     /locus_tag="VVN16_001767"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU5573097.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVVHDNVTKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            7248..7412
                     /locus_tag="VVN16_001768"
     CDS             7248..7412
                     /locus_tag="VVN16_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU5573098.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            7463..>8126
                     /locus_tag="VVN16_001769"
     CDS             7463..>8126
                     /locus_tag="VVN16_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5573099.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVN"
BASE COUNT         2446 a         1577 c         1849 g         2254 t
ORIGIN      
        1 ttttgagctg acttcgtcag ttctatccac aacctcaaaa cggtgttttg agctgacttc
       61 gtcagttcta tccacaacct caaaacggtg ttttgagctg acttcgtcag ttctatctac
      121 aacctcaaag cactgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
      181 cattgagtat tagaacatac aatggaggtc gtcatggaca atatcatcga tgtgtcaatt
      241 cctgttgcag aagtggtgga caagcatcca gaagtcttgg aaattctagt ggagttgggt
      301 tttaaacccc ttgccaatcc cttaatgcgc aatacagttg gtcgtaaagt atcacttaaa
      361 cagggttcta agctagcagg aactcctatg gacaagattg tacgcacact ggaagcgaat
      421 ggctacgaag tgattggatt agactaatgg cagatgaacg gattcatgtc ctacgggata
      481 ttttgttaga attgcacaat ggcgcctctc ctgagtcggt tcaagatcgc tttgatgcga
      541 cctttacggg cgtgtcagcc atcgagattt cccttatgga gcacgagctg atgaactcgg
      601 attcgggcgt cacttttgaa gatgttatgg aactctgtga tgtccatgcc aatcttttta
      661 aaaatgctat caaaggtgtc gaagtttcag atactgagca tccaggtcac ccagttcgtg
      721 tcttcaaaga agaaaatctg gctctccgtg cagctttgat tcgcattcgt agattgttag
      781 atacctatga gttgatggaa gacgaggaaa tgcttgcgga gatgcgcaag ggcttggtgc
      841 gtcagatggg gcttgtgggg caatttgaca tccattacca acgtaaggaa gaactcttct
      901 ttcctatcat ggagcgctat ggacacgatt cacctcccaa agttatgtgg ggagtggatg
      961 atcagattag ggaactcttt caaacagctc taacgacagc caagtcacta ccagaagtgt
     1021 caattagcag tgtaaaggaa gcttttgaag cttttgcgac agagtttgaa agtatgattt
     1081 tcaaggaaga gtccatcctc ctcatgattc tccttgagtc ttttactcag gatgactggc
     1141 ttcagattgc ggaggagagc gatgcctatg gctatgccat catccgtccg tcagagaaat
     1201 gggtgccaga acgacagagc tttattgagg aaaagattgc agaggaacct gtacagctag
     1261 atacggcaga aggtcaagtt caacaagtca tagatacgcc agaaggccat tttaccatta
     1321 cctttacccc taaggaaaag gaagctgtgc tggaccgcca tagtcaacag gcttttggta
     1381 atggctatct ttcagtcgag caggccaatc tcatcctcaa tcatctccct atggagatta
     1441 cctttgtcaa taaagaagat attttccagt attacaatga caatacgcca gctgatgaga
     1501 tgattttcaa acggacgccg tcccaagtcg ggcgcaatgt cgaactctgc catccgccta
     1561 agtacttgga caaggtcaaa actatcatga aggggcttcg tgagggaagc aaagacaagt
     1621 atgaaatgtg gttcaagtct gagtcgcgag gtaagtttgt ccacatcacc tatgctgcag
     1681 tacacgatga agacggagaa ttccaaggag tgttggagta tgttcaggat atccagccct
     1741 accgtgagat tgatacggac tattttcgtg gattagaata aggagaaaaa atgagttacg
     1801 aacaagaatt tatgaaggaa tttgaagctt gggtcaatac ccaaatcatg attaacgata
     1861 tggcgcacaa ggaaagtcaa aaagtttacg aagaagacca ggacgagcgt gccaaagatg
     1921 ccatgattcg ctacgagagt cgcttggatg cttatcagtt cttgcttggt aagtttgaaa
     1981 acttcaaagc aggcaaggga ttccatgatt tgccagaagg cttgtttggt gagcgaaatt
     2041 attaaacgag aaagattctt gatttttcac taaaatcttg atagaatgtt tatgttaaat
     2101 ccttgtcaga gcagggattt tttattgaaa ggattttatc atgtcaaaga aactcaatcg
     2161 taaaaaacaa ttacgaaatg gcctccgtcg cgcaggtgcc ttttcaagta ctgtgactaa
     2221 ggttgtagat gagacaaaaa aagtcgtgaa gcgtgcagaa cagtcagcaa gcgcagctgg
     2281 taaggctgtt tctaaaaaag ttgaacaagc agtagaagct accaaagagc aagctcaaaa
     2341 agtagctaat tctgtagaag attttgcagc aaatttgggt ggacttccac ttgatcgtgc
     2401 caagactttc tatgatgaag gaatcaagtc tgcttcagat ttcaaaaact ggactgaaaa
     2461 agaactcctt gccttgaaag gaatcggccc agctaccatc aagaaattga aagaaaatgg
     2521 catcaagttc aagtaatttt tcttgagcct tgcatttccg aaaaaatctt gctacaatag
     2581 agccattaga ggtgttttga atcccacatt ttacagaaag tggcggcgct gagaagtcca
     2641 caaatgtgtc aaaactggtt gctaatggat gaaaaattga aataaaagtg tctttttgct
     2701 ttaaagacga gagttgcggg caactgcccg aaattgggtg gtaccgcgga taaacacatt
     2761 cgtccctgtc atgtagatgg cagggctttt cttttacgtc ttagtcaaag ggggttgtta
     2821 tgaagcaatc ttttaacaca agtaaactat attatggttt tcctgttttt attttaggat
     2881 atcaagatta gaattttggc tataacatca cgacctgtag ttcctcttat agtctaggag
     2941 attgggttgt gattggagtc gttgcgagag agaatgccgc agagcagatc aaacagtatc
     3001 aaaaatttac tgtgaatatt tctgatgaaa cttctatgct tgcgatggag caggctggtt
     3061 ttatcagtca tcaggagaaa ttggaacgtt tgggagtgca ttatgaaatt tctgaacgaa
     3121 ctcagactcc tattttagac gcctgtccac ttgttttaga ttgtcgggta gataggattg
     3181 ttgaggaaga cggtatttgc cacatctttg ccaagattct tgagcgactt gttgttccag
     3241 aactcctgga tgaaaaggga cattttaaaa atcaactgtt tgccccaacc tatttcatgg
     3301 gagatggata tcagcgcgtt tatcgctatc tggacaagcg tgtagatatg aagggcagtt
     3361 tcatcaaaaa agcgaggaag aaggatggca agaactgagc taccagataa aatcgaaaca
     3421 gaatgtctcg ttttacgagt ccgtactgtg gcggatgctg aggatatctt tgactatgct
     3481 agtttgccag aggtcgccta tccagcaggt tttcctccag tcaagacctt ggaagatgag
     3541 atttattatc tggagcacat tcttccggag cgtaatcaaa aggaaaatct cccagcaggc
     3601 tacgggattg tcgtcaaagg aacggataag atcgttggct ctgtcgattt caaccatcgc
     3661 catgaagatg atgtgctgga aatcggctat accttacacc cagactattg ggggcgaggt
     3721 tatgtgccag aagctgcgcg tgccttgatt gacttagcct ttaaagattt gggtcttcac
     3781 aagattgaac taacttgctt tggatataac cttcaaagtc aacgagtcgc ggaaaaactt
     3841 ggctttaccc tcgaagctcg cataagagac cgcaaagatg ctcaaggcaa ctgctgtgat
     3901 gatttgagat atgcctttct gaagagtgag tgggaggtga tttgatgcat cttttcatca
     3961 ttggtgctcc agcatcagga aaaatgacca ttggtcaaga attatctcga ctgacggatg
     4021 ctaccctctt ttataaccat caagccattg attttgcact agaaatctat caggactata
     4081 cagaggaaat gtgggaattt gttcgtggaa tcactttttc attccttgga gcgagtgcaa
     4141 gaaatcagcg atcagtaatt ttaacagatg taattgattt ttcaaatcag tatcatctga
     4201 tatatttaaa gcaaattcag agtttgttgg atgattatca tcaagagatt ctttttgttg
     4261 agttggaaac aagtcttgaa gagtgcatac gtcgaaatcg aacggagaat cgattaaagt
     4321 acaaaccctt aaaacgacat attgaggtat ctgaaagaga aattatagag accgctgaaa
     4381 cacttcaatt aaattcccaa catcaaccga atgagttgaa ccactacttt aaaataaata
     4441 atacgaattt gtctgcagaa gaagctgcta agcaaattca aaataaaata aacaaaatag
     4501 agaaaggaca cacacatgtc taaagaactt tcacctaaat acaatccagc cgaggttgag
     4561 gctggtcgtt atcaaaaatg gcttgatgct gatgttttca agccttcagg cgatcaaaag
     4621 gctaagcctt attcaatcgt tattccacca ccaaacgtta caggtaaact tcaccttggt
     4681 cacgcttggg atacaacttt gcaagatatt atcatccgtc aaaaacgcat gcaaggtttt
     4741 gatacccttt ggcttccagg aatggaccac gctggtattg cgactcaggc taaggttgag
     4801 gagcgcttgc gtggtgaggg catttcccgc tatgaccttg gtcgtgagtc tttcttgacg
     4861 aaagtctggg aatggaaaga cgaatatgcc actaccatca aggaacaatg gggcaagatg
     4921 gggctctctg tagactattc tcgtgagcgt ttcactcttg acgaaggttt gtcaaaagct
     4981 gttcgtaagg tctttgtgga cctttacaag aaaggctgga tctaccgtgg tgagtttatc
     5041 atcaactggg acccagcagc tcgcacagcc ctttctgata ttgaggtgat tcacaaggat
     5101 gtggaaggtg ccttctacca catgaattac atgctggaag atggttcacg cgcccttgaa
     5161 gttgctacaa ctcgtcctga gactatgttt ggggacgttg cggttgcggt taatccagaa
     5221 gacccgcgct acaaggactt gattggtaaa aacgtcatcc ttccaatcgc taataaactc
     5281 atcccaatcg ttggagatga gcacgcagat cctgagtttg gtactggtgt cgtgaaaatc
     5341 acacctgccc acgatccaaa tgacttcttg gttggccaac gtcataactt gccacaagtc
     5401 aacgtcatga acgacgacgg aactatgaat gagcttgcct ttgaattttc aggcatggat
     5461 cgttttgaag ctcgtaaggc agtcgttgct aagttggaag aaatcggtgc ccttgtcaaa
     5521 atcgaaaaac gtgtccattc agtcggtcac tcagaacgga caggtgtcgt agttgagcca
     5581 cgcttgtcta ctcaatggtt cgtcaagatg gaccaattgg ctaagaacgc cattgccaac
     5641 caagacacag aggacaaggt cgaattctac ccacctcgtt tcaacgatac cttccttcaa
     5701 tggatggaaa atgtccacga ctgggttatc tctcgtcagc tttggtgggg tcaccaaatc
     5761 cctgcctggt acaatgctga tggtgaaatg tatgtcggcg aagaagctcc agaaggtgac
     5821 ggatggactc aggacgaaga cgtcttggat acttggttca gttctgccct ctggccattt
     5881 tcaaccatgg gctggcctga agtcgactca gaagacttta aacgttattt cccaacttca
     5941 accttggtaa caggttacga catcatcttc ttctgggtgt ctcgtatgat cttccagtca
     6001 ttggaattca caggccgtca accattccaa aacgtcctta tccacggtct tattcgtgac
     6061 gagcaaggac gcaagatgtc taagtctctc ggtaacggga ttgacccaat ggatgttatc
     6121 gagaaatacg gtgccgatgc ccttcgatgg ttcctttcaa acggttctgc gccaggacaa
     6181 gacgtgcgtt tctcttatga gaaaatggat gcttcatgga acttcattaa caagatctgg
     6241 aacatctctc gctatatcct tatgaacaat gaaggtttga cgctggatgt ggtgcatgac
     6301 aatgtcacaa aagttgcaac aggtgaggct ggtaatgtga cggaccgctg gattctccac
     6361 aacctcaatg aaacgattgg aaaagcaact gctaactttg ataagtttga gtttggtgtt
     6421 gctggccaca tcctctacaa cttcatttgg gaagaatttg ccaactggta tgttgagttg
     6481 accaaggaag ttctttacag cgacaatgaa gacgataagg tcattactcg atctgttctc
     6541 ctctatacgc tggataaaat cctccgtctt cttcacccaa tcatgccatt cgtgacagaa
     6601 gaaatctttg gtcagtacgc tgaaggctct atcgtgacag cagcataccc aactgtcaac
     6661 ccagcctttg aggaccttgc tgcccacact ggtgtggaaa gtctcaaaga cttgatccgt
     6721 gccgttcgta atgcgcgtgc ggaagtaaat gtagcaccaa gcaaacctat caccatcctt
     6781 gttaagacaa gcgatagcga cttggaagcc ttctttaaca gcaatgtcaa ctacatcaaa
     6841 cgctttacaa atccagaaca tttggaaatc gcatcaacca tccctgcacc tgaactggct
     6901 atgtcaagtg tcatcacagg agcagaaatc tacctgccac tggccgatct cctcaatgtt
     6961 gaagaagaac tggcccgtct cgacaaggaa cttgctaaat ggcaaaaaga actggacatg
     7021 gttggtaaga aactctctaa cgaacgcttc gtagccaacg ccaaaccaga agtcgtccaa
     7081 aaagaatgcg acaaacaagc tgactaccaa gctaaatatg acgcgaccgt cgcacgtatt
     7141 gatgagatga agaagttggt gaaatgatgt taaaaaccta accctctcgg gttgggtttt
     7201 acatttgata atgttataat aaaaaggaga aactttagga gaatactatg gggaaaattg
     7261 ttgctattga tttatttagc ggtgcaggtg gcactacaag tggccttaaa aaatcaggca
     7321 tagatgttca agttgctgtt gaaattgatt ccgtagctgt taaaacttat aagttaaata
     7381 atcctgaggt gtctgtaata gatatggagt aaataaaact tcaattttac aagctttata
     7441 tggttctcca gccaataaaa gtgtgtctga atattggttt agtacaaatg ttgatcaaat
     7501 agatgaagtg gatgggaaac aatgtttaat ctattcatat tataacgtta aagcttctag
     7561 aaatgtagaa gttcttaaag gccgttctgg aacaaaaaaa ggattagatt attgggaacc
     7621 atatgctcct caaaaacaat atgagatgga aagattaccc aaaaataaat atattggaag
     7681 ttcttcgaca gacagatggg atggcattga aaaaaatgtt gtcttttgtg attgtaaaga
     7741 atatgtttct gcttttgatt tatttttcta tcactataat tttaaaaaaa tttctactca
     7801 acgtagtaaa caagatttta taagattacg atctaaacca gttgctgata ttttaaagaa
     7861 taatacaagt agctatacgc gttataaaaa ggaaatggtt atagataatg ttaaagttga
     7921 tgataaagtt tgtgaaatta ttagtgaaat aatggatgaa tcttatacag acattcaaat
     7981 tttaacacat aagttgtatt caaaaggtga tgatataaaa gcttccaaaa ctatttggat
     8041 gaaaaaatct ggaaaagaat attctgaggc ttttgcagga acaggtgagg ctagaattat
     8101 tttgttagta aatgatatag taaatg
//
LOCUS       DAVHIT010000088         8071 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVHIT010000088 DAVHIT010000000
VERSION     DAVHIT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.090"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..692
                     /locus_tag="VVN16_001770"
                     /pseudo
     CDS             1..692
                     /locus_tag="VVN16_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            753..1715
                     /locus_tag="VVN16_001771"
     CDS             753..1715
                     /locus_tag="VVN16_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5573100.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYEDTSISVSSNETDKV
                     LAGSLYTDTNEQGLMIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1892..2899
                     /gene="gap"
                     /locus_tag="VVN16_001772"
     CDS             1892..2899
                     /gene="gap"
                     /locus_tag="VVN16_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5573101.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2967..3803)
                     /locus_tag="VVN16_001773"
     CDS             complement(2967..3803)
                     /locus_tag="VVN16_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5573102.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3929..6124
                     /locus_tag="VVN16_001774"
     CDS             3929..6124
                     /locus_tag="VVN16_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU5573103.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWCRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            6177..6329
                     /gene="rpmG"
                     /locus_tag="VVN16_001775"
     CDS             6177..6329
                     /gene="rpmG"
                     /locus_tag="VVN16_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5573104.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6339..6515
                     /gene="secE"
                     /locus_tag="VVN16_001776"
     CDS             6339..6515
                     /gene="secE"
                     /locus_tag="VVN16_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU5573105.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6570..7106
                     /gene="nusG"
                     /locus_tag="VVN16_001777"
     CDS             6570..7106
                     /gene="nusG"
                     /locus_tag="VVN16_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU5573106.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            7228..7707
                     /locus_tag="VVN16_001778"
     CDS             7228..7707
                     /locus_tag="VVN16_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5573107.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            7801..7872
                     /locus_tag="VVN16_001779"
     tRNA            7801..7872
                     /locus_tag="VVN16_001779"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7834..7836,aa:Glu,seq:ttc)
BASE COUNT         2582 a         1383 c         1748 g         2358 t
ORIGIN      
        1 aaaggaacaa aaccaagaaa agttgataga gatagactta aaaactatct tactgacaat
       61 ccagatgctt atttgactga aatagcttct gaatttggct gtcatccaac taccatccac
      121 tatgcgctca aagctatggg ctacactcga aaaaaaagaa ccacacctac tatgaacaag
      181 acccagaaaa agtagcctta tttcttaaga attttaatag tttaaagcac ctagcacctg
      241 tttagattga cgaaacagga ttcgatactt atttttatcg agaatatggt cgctcattaa
      301 aaggtcagtt aataagaggc aaagtatctg gaagaagata tcagaggatt tctttggttg
      361 caggtctaac aaatggtgaa ttaatcgctc caatgactta cgaagagacg atgacgagcg
      421 acttttttga agcttggttt cagaattttc tcttaccaac attaaccaca ccatcggtta
      481 ttattatgga taatgcaaga ttccatagaa tgggtaagtt agaactttta tgcgatgagt
      541 ttgggcataa acttttacct cttcctccct actcacctga gtacaatcct attgagaaaa
      601 catgggctta tatcaaaaag cacctcaaaa aggtattacc aagttgcaat accttttacg
      661 aggctctttt gtcttgttct tgtttcaatt gactatagtt cacggataca gttgggaaag
      721 aagctattaa ttatttggag agtccttttg ggatgaacat gtcaaatatt actatagatg
      781 ggcaagaaat ggatctgagt caaacgatgc cttcttatgc tcaagtagtg agtctttatg
      841 aggatacaag catctctgtt agtagtaatg agacggacaa agtgttggct ggatccctct
      901 atactgatac aaatgaacag ggattaatga ttccaagcag tttactaaaa aattggaatg
      961 aacagacagg aaaaaatttg acagctaatg atcttattgg caaatcagtc tcagccagca
     1021 ttgtagaaag tgctgccgaa gctagtaaga ttgctcaatt tcaaacgaag attgtacgtg
     1081 taatcaatga tgaagatgac atggaggaca gcaacagttt catgctgtct catcaaatgg
     1141 aaacgatttt gaaagaggct ggatttacga aagctgtatc ttattttatc ttggaactca
     1201 aagatccatc acagacaaaa gtagtaacag aagaattaca gaaaaataag aagtatactg
     1261 tgctttctca acagagagtt cttgatattg tgattacctt tattcgtgtt attcagggat
     1321 tattgattgt gctttcatca caagctattg tggtagcagc ggttatgatt ggtatcatta
     1381 tttacatcaa tatcatgcaa cgttccaagg aaataggtgt catgaaagca gttggttatc
     1441 agaatcgtgg tgtcaaagga atttttattt acgaggctat ctggattgta ggcatcgcct
     1501 tgctgctggc atttttggtt gcacaagggg tgggaagttt ggcgaatgcg attgtaagtc
     1561 acttttaccc atccatcact aaggtttttg aattaaatct tttatctgtt ttaggaactc
     1621 tagttttcgc tctattactt ggttatgtct cagcctactt cccggcgcgt aagattagta
     1681 aaatggatcc tgtagaatcg ttacgctatg aataataagg gattccttga tttacagtct
     1741 taaaaagaaa ctcacataga aaaacaatgg ccatacttgc aattcaactc aaatagttgt
     1801 ataataggtt tgttgaaagg tgatttgtag tgattcaagt tactcttttc acaataaaaa
     1861 tttcatgatt ttcataagga ggaaatcact aatggtagtt aaagttggta ttaacggttt
     1921 cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac
     1981 acgcatcaac gaccttacag atccagttat gcttgcacac ttgttgaaat acgacacaac
     2041 tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt ggatttgaag ttaacggtaa
     2101 attcatcaaa gtttctgctg aacgtgatcc agaacaaatc gactgggcta ctgacggtgt
     2161 agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa gaagcagctg aaaaacacct
     2221 taaaggtgga gctaaaaaag ttgttatcac tgctcctggt ggaaacgacg ttaaaacagt
     2281 tgtattcaac actaaccacg acgttcttga cggtactgaa acagttatct caggtgcttc
     2341 atgtactaca aactgcttgg ctccaatggc taaagctctt caagacaact ttggtgttgt
     2401 tgaaggattg atgactacta tccacgctta cactggtgac caaatgatcc ttgacggacc
     2461 acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct gcaaacatcg ttcctaactc
     2521 aactggtgct gcaaaagcta tcggtcttgt aatcccagaa ttgaatggta aacttgatgg
     2581 atctgcacaa cgcgttccaa ctccaactgg atcagttact gaattggtag cagttcttga
     2641 aaagaacgtt actgttgatg aagtgaacgc agctatgaaa gcagcttcaa acgaatcata
     2701 cggttacaca gaagatccaa tcgtatcttc agatatcgta ggtatgtctt acggttcatt
     2761 gtttgacgca actcaaacta aagttcttga cgttgacggt aaacaattgg ttaaagttgt
     2821 atcatggtac gacaacgaaa tgtcatacac tgcacaactt gttcgtactc ttgaatactt
     2881 cgcaaagatt gctaaataat tcttgagttg atagaaagca aggctttgtg gtcttgcttt
     2941 tttatatgga aaaatggatg acacgatcat ccattctttt ttaattcttt ttcaaatgta
     3001 tttgaaaggg tagtgaaagt tagcctctct aaagtaagtg ggtgggtaaa ggaaagtcgg
     3061 aaggcatgaa gcataagccg gcttgtcttt gatttactat tatagagagg gtctcccagg
     3121 ataggaagat tatgatgcaa aaggtgcaca cgaatctgat gggttcgccc tgtctttagc
     3181 ttgcaatgag ccaaggaagt cttgtttgag aattgcttta atctgcttac atgcgtttca
     3241 gcatatttcc cattttttgc atcaactatt ctttttctac gatcatggcg atcacgtcca
     3301 attttgtctc tgaaaacaag ttcttttctg ttgatatttc catcaactag agcccaatat
     3361 tctctagaaa tctctttttt ctccaataag cgattgagaa tgggcaggat aaaaggattt
     3421 ttggcaaaga gaactaagcc actggtttcc atgtccagac gatgaacgac atagcaggtt
     3481 tggccaacat aggtactgac atggttaaga agggcaattt cgtttggttg attaccatgc
     3541 gttttcatcc cctctggttt gtttacaata atcaagtgtt gatcttgata aacttcctgc
     3601 actaagtctg ggttgcccca agggatcgtc ttttggggat aatcttcctc gtcaaaagtc
     3661 aactggcaaa catctccagg atttacgatt tcgttccagc ggacttcttc ttgatttatc
     3721 aaaatatgtt tcttgattct caaaaaatga cggatttttc tagggatgag gagttgttcc
     3781 tcaagtaatt gctttaccgt catttgaggt agagaggcgg gtaatgtaaa tgtgaattgc
     3841 atacagatat tgtaacaaaa aaagccctat ttggatagga aatagctaaa ttcttgtctt
     3901 cctatgatga agatgataaa ataaacgcat gaaattagat aaattatttg agaaatttct
     3961 ttctcttttt aaaaaagaaa caagtgaact agaggactct gattctacta tcttacgtcg
     4021 ctctcgtagt gatcgaaaaa aattagccca agtaggtccg attcgaaaat tctggtgtcg
     4081 ttatcatcta acaaagatta tccttatact aggtttgagt gcaggcttgc tagttggaat
     4141 ctatttgttt gctgtagcca agtcgaccaa tgtcaatgat ttgcaaaatg ccttgaaaac
     4201 tcggactctt atttttgacc gtgaagaaaa agaggctggt gccttgtctg gtcaaaaggg
     4261 aacctatgtt gagctgactg acatcagtaa aaacttgcag aatgctgtta ttgcgacaga
     4321 agaccgttct ttctataaaa atgacgggat taactatggc cgtttcttct tggctattgt
     4381 cactgctgga cgttcaggtg gtggctctac cattacccaa cagctggcta aaaacgccta
     4441 tttatcgcag gatcaaactg ttgagagaaa agcgaaagaa tttttccttg ccttagaatt
     4501 aagcaaaaaa tatagtaagg agcaaattct aaccatgtac cttaacaacg cttattttgg
     4561 aaatggtgtg tggggtgtag aagatgcgag taagaaatac tttggagttt ctgcatcaga
     4621 agtgagtctg gatcaagctg cgactctggc agggatgctc aaggggccgg aactgtataa
     4681 tcccttgaat tccgtagaag attctactaa tcggcgcgat actgtcttgc agaatatggt
     4741 tgcagcagga tatattgata aaaaccaaga aaccgaagct gctgaagttg atatgacttc
     4801 gcaattgcac gataagtatg aaggaaaaat ctcagattac cgttacccct cttattttga
     4861 tgcggtggtt aatgaagctg tttccaagta taatctaaca gaggaagaga ttgtcaataa
     4921 tggctaccgc atttacacag agctggacca aaactaccaa gcaaatatgc agattgttta
     4981 tgaaaacaca tcgctatttc cgagggcaga ggatggaacg tttgctcaat caggaagtgt
     5041 agctctcgaa ccgaaaacag ggggagttcg tggagttgtc ggtcaagttg ctgacaatga
     5101 taaaactgga ttccggaatt tcaactatgc aacccaatca aagcgtagtc ctggttctac
     5161 aattaagcct ttagttgttt atacaccagc agttgaagca ggctgggctt tgaataagca
     5221 gttggataac cataccatgc agtatgatag ctataaggtt gataactatg cagggatcaa
     5281 aacaagtcga gaagttccta tgtatcaatc cttggcagaa tcgcttaatc tacctgctgt
     5341 tgccactgtt aatgatttgg gtgttgacaa ggcttttgag gcaggcgaaa aattcggact
     5401 caacatggaa aaggtcgacc gtgttcttgg tgtcgccttg ggaagcggtg ttgaaaccaa
     5461 ccctcttcaa atggctcaag catacgctgc ctttgcaaat gaaggtttaa tgcctgaagc
     5521 tcattttatt agtagaattg aaaatgctag tggacaagtt attgcgagtc ataaaaattc
     5581 acaaaaacgg gtgattgata agtctgtagc tgacaagatg accagtatga tgttggggac
     5641 tttcaccaac ggtaccggta ttagttcatc gcctgcagac tatgtcatgg cagggaaaac
     5701 tggaacaact gaagcagttt tcaatccgga gtacacaagt gaccagtggg taattggtta
     5761 tactccggat gtagtgatta gccactggct tggctttccg accactgatg aaaatcacta
     5821 tctagctggc tctacttcaa acggtgcagc tcatgtcttt agaaacattg ccaataccat
     5881 tttaccttat acgccaggaa gtacctttac ggttgaaaat gcttataagc aaaatggaat
     5941 tgcaccagcc aatacaaaaa gacaagtaca aaccaatgat aatagccaga cagatgataa
     6001 tttgtctgat attcgagggc gtgcgcaaag tctagtagat gaggctagcc gggctatctc
     6061 agatgcgaag attaaggaaa aggctcaaac aatatgggat tcgatagtca atctatttcg
     6121 ctaagatgct tgtcaaagcc tagctttctt gttataatgg ataagatgga ggcgttatgg
     6181 cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc gagaaactat tcaatcaaga
     6241 tcagcggaaa ccccaagcct acacgactag aagtaaataa attttgtaag cattgtggca
     6301 agtacactac acacagagaa acgagatagg agagagcgat gcgttttatt ggagatattt
     6361 ttagacttct taaagacaca acatggccaa ctcgcaagga aagctggaga gattttcgtt
     6421 ctatcatgga atacacagct ttctttgtag taattattta catttttgac cagttgattg
     6481 tttcaggttt gattcgattt attaacattt tttagaagat tggtggagtt aattacacta
     6541 gaaatcttct atttatgaaa ggaaatatca tggatagttt tgataaagga tggtttgttt
     6601 tacaaactta ttctggttat gaaaataagg tgaaagaaaa tctattacaa cgtgcacaaa
     6661 cctacaatat gttggataat attctacgcg ttgaaattcc aacacaaaca gtgcaagttg
     6721 aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt tccaggttat gttcttgtag
     6781 aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa cacaccaaat gttacaggat
     6841 ttgtcggatc acacgggaac agatcaaaac caactccatt attggaacaa gaaattcgtg
     6901 acattttggt atctatggga caaactgttc aagaatttga tttcgatgtt gagattggtc
     6961 aaactgtacg tattattgat ggtgcttttg cagactacac tggtaagatt acagaaattg
     7021 ataataacaa agtgaaaatg attatctcta tgtttggtaa tgacacagtt gcagaagtaa
     7081 acctaaacca aatcgcagaa ttataaccct gagagaggct ggagcctctc ttttttgtgc
     7141 agtttaggag ctaaagggaa cagaatggag aaaatggaac aaatgtgttt tctaatctgt
     7201 tagactgtat ctagaaaggg gaaaattatg attaaagaat tgtatgaaga agtccaaggg
     7261 actgtttata agtgtagaaa tgaatattac cttcatttat gggaattgtc ggattgggac
     7321 caagaaggca tgctctgctt acatgaattg attagtagag aagaaggact ggtagacgat
     7381 attccacgtt taaggaaata tttcaaaacc aagtttcgaa atcgaatttt agactatatc
     7441 cgtaagcagg aaagtcagaa gcgtagatac gataaagaac cctatgaaga agtgggagag
     7501 atcagtcatc gtataagtga gggaggtctc tggctagatg attattatct ctttcatgaa
     7561 acactaagag attatagaaa caaacaaagt aaagagaaac aagaagaact agaacgcgtc
     7621 ttaagcaatg aacgatttcg agggcgtcaa agagtattaa gagacttacg cattgtgttt
     7681 aaggagttta ctatccgtac ccactaggaa gtcatgcaaa aaaaatggaa aaaattagaa
     7741 aaagtagttg acaaagtttg aaaagactgt ataatagtaa gagttgaaaa taacaactca
     7801 ggtccgttgg tcaaggggtt aagacaccgc cttttcacgg cggtaacacg ggttcgaatc
     7861 ccgtacggac tatggtatgt tgcggttgga acacttgatg aaaaaaagtt taaaaaagct
     7921 taaaaatctt aaaaaaagtg ttgacaagtg aaagtggctg tgatatacta atatagttgt
     7981 cgcttgagag aagcaagtga caaagacctt tgaaaactga acaagacgaa ccaatgtgca
     8041 gggcgctaca acgtaagttg tagtactgaa c
//
LOCUS       DAVHIT010000089         7762 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVHIT010000089 DAVHIT010000000
VERSION     DAVHIT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.091"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            36..725
                     /gene="coaB"
                     /locus_tag="VVN16_001780"
     CDS             36..725
                     /gene="coaB"
                     /locus_tag="VVN16_001780"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU5573108.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISTDQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            737..1288
                     /gene="coaC"
                     /locus_tag="VVN16_001781"
     CDS             737..1288
                     /gene="coaC"
                     /locus_tag="VVN16_001781"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU5573109.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLITPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1272..1835
                     /locus_tag="VVN16_001782"
     CDS             1272..1835
                     /locus_tag="VVN16_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5573110.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2117..2644
                     /locus_tag="VVN16_001783"
     CDS             2117..2644
                     /locus_tag="VVN16_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5573111.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2656..3171
                     /locus_tag="VVN16_001784"
     CDS             2656..3171
                     /locus_tag="VVN16_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU5573112.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3168..3635)
                     /locus_tag="VVN16_001785"
     CDS             complement(3168..3635)
                     /locus_tag="VVN16_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU5573113.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPDTNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWIQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKEIF"
     gene            complement(3708..4196)
                     /locus_tag="VVN16_001786"
     CDS             complement(3708..4196)
                     /locus_tag="VVN16_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573114.1"
                     /translation="MVITVKQMETPEEIEGKSLVHWKAWRESYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAMDGLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALDHFSEIFLWVLKDNKRAIAFYQKMGYTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(4162..4725)
                     /locus_tag="VVN16_001787"
     CDS             complement(4162..4725)
                     /locus_tag="VVN16_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573115.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNFGMMKLAEKLRMKKEAHIPKVRYYQGVY
                     YDSIKYGILREEWEEANGHHCQTDGNS"
     gene            complement(4735..6723)
                     /gene="uvrB"
                     /locus_tag="VVN16_001788"
     CDS             complement(4735..6723)
                     /gene="uvrB"
                     /locus_tag="VVN16_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU5573116.1"
                     /translation="MINRITDNKFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVSKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(6800..7753)
                     /locus_tag="VVN16_001789"
     CDS             complement(6800..7753)
                     /locus_tag="VVN16_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5573117.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVALGQYRLDSIHLDTYSLAF
                     TLFTIPFWILQGTAEELVTRAWLIPQLAKRTNLKVAIIISSSLFTLLHLGNPGITFLS
                     AIDLFLFGVAMSLYLLKTDTIWGIGGIHGAWNFAQGNLFGVLVSGQSSGTSIMKFTPQ
                     GNQDWLSGGSFGIEGSIVSSIILFLLILYLAYQLKKENERM"
BASE COUNT         2306 a         1733 c         1385 g         2338 t
ORIGIN      
        1 tttacttacc gcttattttt gtgtacaata gtgctatgaa aattttagtt acatcgggcg
       61 gtaccagtga agctatcgat agcgtccgct ctatcactaa ccattctaca ggtcacttgg
      121 ggaaaattat cacagagact ttgctttttg cagggcatga agtttgttta attacgacaa
      181 aacgagctct gaagccagag cctcatccta acctaagtat tcgagaaatt accaatacca
      241 aggaccttct aatagaaatg caagaacgtg ttcaggatta tcaggtcttg atccactcaa
      301 tggctgtttc tgactacact cctgtttata tgacagggct tgaggaagtt caggctagct
      361 ccaatctaaa agaattttta agcaagcaga atcatcaggc caagatttct tcaactgatg
      421 aggttcaggt tttgttcctt aaaaagacac ccaaaatcat atccctagtc aaggaatgga
      481 atcctactat tcatctgatt ggtttcaaac tgctggttga tgttaccgaa gatcatctgg
      541 ttgacattgc acgaaaaagt cttatcaaga atcaagcaga tttaatcatc gcgaatgacc
      601 tgactcaaat ttcaacagat cagcaccgag ctatatttgt tgagaaaaat cagcttcaaa
      661 cagtccagac taaagaagaa attgcagaac tcctccttga aaaaattcaa gcctatcatt
      721 cttagaaagg aaaactatgg caaacattct cttggctgta acgggttcaa tcgcctctta
      781 taagtcggca gatttagtca gttctctaaa aaaacaaggc catcaagtca ctgtcttaat
      841 gactcaggct gctacagagt ttatccaacc tttgacacta caggtactct cacagaatcc
      901 tgtccacttg gatgtcatga aggaacccta tcctgatcag gtcaatcata tcgaacttgg
      961 aaaaaaagca gatttattta tcgtggtacc tgcaactgct aacactattg caaaactagc
     1021 tcacggattt gcggacaaca tggtaaccag tacagctcta gccctaccaa gtcatattcc
     1081 caaactaata actcctgcta tgaatacaaa aatgtatgac catccagtaa ctcagaataa
     1141 tctgaaaaca ttagaaacct acggctatca gctgattgct cctaaggaat ccctactagc
     1201 ttgtggagac cacggacgag gagctttagc tgacctcaca attattttag aaagaataaa
     1261 ggaaactatc gatgaaaaaa cgctctaata ttgcacccat tgctatcttt tttgctacca
     1321 tgctcgtgat acactttctg agctcactta tctttaacct ttttccattt ccaatcaaac
     1381 cgaccattgt tcatattcct gtcattattg ccagcattat ttatggtcca cgagttgggg
     1441 ttacacttgg atttttgatg ggattactta gcttgacggt taacacgatt acgattctac
     1501 cgacaagcta cctcttctct cccttcgtac caaacggaaa catctactca gctatcattg
     1561 ccattgtccc acgtattttg attggtttaa ctccttactt agtctataaa ctgatgaaaa
     1621 acaagactgg tctgatttta gctggggccc ttggttcctt gacaaatact atctttgtcc
     1681 ttggaggaat cttcttccta tttggaaatg tttataatgg aaatatccaa cttcttctgg
     1741 caaccgttat ctcaacaaat tcaattgctg aattggtcat ttctgcaatt ctaaccctag
     1801 ccattgttcc acgactacaa accttgaaaa aataaaaaca atctccgctt tcaaacctga
     1861 aggtggagat tttgctctat acagtttctt tttaatgaaa tctttactat agtaaaacga
     1921 aataagaaca ggacaaatcg atcaggacag ccaaatcgat ttctaacaat gttttagaag
     1981 tagaggtgaa ctattctagt ttcaatctac tataactttt aactaaaaaa tgatctataa
     2041 acccaagtaa attcttgctt tattgtctgg tttattgtac catactagtg tatatgcagt
     2101 taaaaaggag attcttgtga atacacggaa aaagacacaa tttatgacaa tgacagccct
     2161 tttaacggct attgcgattt tgattccaat tgttatgcct ttcaagattg tcattccacc
     2221 tgcttcctat actttgggga gccacatcgc tatttttata gccatgttct tgtcgccctt
     2281 gatggcagtt tttgtcatcc tagcctctag ttttggattt ttgatggctg gctatcccat
     2341 ggttatcgtt tttagggctt tttcccatat atcttttggt gctttaggag ctctttacct
     2401 acaaaaattc cccgataccc tagataaacc aaaatcttcc tgggttttca actttgtttt
     2461 ggctgttgtt catgcccttg ctgaagtatt agcctgtgtc cttttttacg caacttcggg
     2521 taccaatgta gaaaacatgt tctatgttct atttgtacta gttggatttg gcacaattat
     2581 ccatagtatg gtagactata cattagcact agctgtctat aaagtgcttc gaaaacgccg
     2641 ttaaaaggaa aagctatgac aaaagatcgc aaacaagccc tgctccaact cttgaaagaa
     2701 gctcctaaag cccttaatgg ccaaagtttg gcggaacatt ttcatgtcac acgtcaggtc
     2761 attgtgcagg acattgcaat tttgagagcc gatggcgctc ctatcctatc caccaatcgt
     2821 ggctacatct ataagcaaat tgataccaat ccttatgttc acaaactttt caaagtgaaa
     2881 catgaagttg aagaaatcgg tcaggaactc cttgctattg tcgataatgg cgggcatgtc
     2941 cagaatacct tgattgacca tcccgtttat ggagaaattg aaaccttgct taaactgtct
     3001 tgtcgcagag atgtgcaaca ttttctagaa caagtcgagc attctgactt tagacctcta
     3061 tctgaattga cagatggcgt ccattaccac ctagtcgaag ccgaaacaca acaagacctc
     3121 cactatatcg aggaggcctt ggatcagcta ggttatttag taaaagacta aaaaatttct
     3181 ttctcccaat cgtcttctgt acggcgaggg tagaaaaatt cagatttgtc cggagcttcc
     3241 atcatctcca tcaaaggtag catatcatag gccagatcca agtttggaat ctggtctttt
     3301 tgaatccaag aaacctctcc ttcatctgaa gagcgaaggg taccagagaa ctcagtcgcc
     3361 ttataacaaa cgacaatata gcgcccacct gtatctagtg gccaattttt aatgccgaca
     3421 agttgaggat tttggatagt caaccctgtt tcttcataga tttcacgaat gacagactct
     3481 gcgaaagatt cgccattttc tacatgacct cctggaaagg cataacctga ccagcgattg
     3541 gtgtcaggag agcgatactg catgactacg cgctgagttt cgaggtcttc aatcagacag
     3601 atatttgtta aaattgttaa ttgggaacgg gacataaatt tactcgcttt ctaagttatt
     3661 aaatttttag acttggtacc agtgccatga gcttctactt tttaaaatta tttagaatag
     3721 aataccatcc gtttttcctt tataggcttt ccaagttcaa gtattttttc ttgtccatca
     3781 aaagtataac ccattttttg atagaaagca atggcgcgct tattatcttt caatacccat
     3841 aagaaaattt cagaaaaatg atcaagagca gtcaaagctg ctttcattaa cttttgtgcg
     3901 atgccttttc cgtaatagtc ttttaaaaca tataaagcaa taatttcacc agcttgaata
     3961 gtctcatcac gaaagttgcc ataactgata aaaccaacta ccttcaggcc atccatcgca
     4021 atcaatgtat tttctggata cttttgacta aagagtcgac acctttctaa tgtcatcgtc
     4081 tcttgaaatt ctgcaggcaa aaggtcgtca taagattctc tccacgcttt ccagtgaaca
     4141 agggatttac cttctatctc ttcaggagtt tccatctgtt tgacagtgat gaccatttgc
     4201 ttcctcccat tcttctctca aaatgccgta tttaatacta tcataataaa caccttgata
     4261 ataacgaact tttggaatat gagcttcttt tttcattctt aatttttcag ccaatttcat
     4321 cataccaaaa tttcctgacc aagttgtcaa acccagatgc tccaactcca agtaatcctg
     4381 aaacgtccta tctatccact gcaacatagc aactttccca ataccagtgt tccagaattt
     4441 tttatcataa ataccaattc ccaattccat ccatcttgtt tgtttacata cccaataacg
     4501 cgaaacagtc ccaactagtt tatcatcaac aaaaatacca aggcgatttg agttgcttaa
     4561 aatggattct gatttttgta gttcgaattc tttaaaattt gaaaaatact gataatcatc
     4621 ataataggga gcatcatact gtttccaaac tggattaagt tgtgaataag ccatttccca
     4681 taaagacatc aaatcttctt tctttaactt tcttaaataa atcatactat tcccctaatc
     4741 caacgccttc acttccagca tcatatcacg aatctgagct gctagttcaa agtcaagcac
     4801 ttcgacggct tcttgcattt gtttttcgag tttcttaaca agttctttgc gctcttgttt
     4861 gttgaggcta ttgatatcaa cttccttgtc ttcttcctta gaaactgcct tggtcacagc
     4921 aatcaggtca cggatttctt tcttgattgt ctgtggtacg ataccatgct cttcattata
     4981 ggccatctgg attttgcgac ggcgggcagt ttcatcgata gcacgttgca tagactgggt
     5041 aaccgtgtcc gcatacatga taacatgacc ttcactgtta cgggcggcac gtccaatggt
     5101 ctggatgagt ccacgttcgt tacgaaggaa accttccttg tcagcatcaa gaatcgctac
     5161 gaggctcact tcaggaacgt caatcccttc acgaaggagg ttgattccga ccaagacatc
     5221 aaagacaccc aagcgcaggt cacggataat ctcagtccgc tccaatgtct tgatatccga
     5281 gtgcatgtac ttgaccttga tacccatttc cttgaagtag tcggtcaagt cttctgccat
     5341 tttcttggtc aacgtggtga taaaggttcg ttcgttcttc tcaacacgag cattaatttc
     5401 acctaagagg tcatcaatct gtcccatagt cggacggact tccacctctg gatccaagag
     5461 tcccgttgga cgaatgattt gctcaatcac tgtctcagtc tgttcatttt catagtcacc
     5521 aggtgtcgct gaaacgtaaa caatctggtg cacatggctt tcaaactcct cccgacggag
     5581 aggacgattg tccagagcag acggcaaacg gaaaccgtaa tttactaaca tttccttacg
     5641 agaacggtct ccattgtaca tgcccttgat ttgtcccatg gtcatgtggc tctcgtcaat
     5701 catgattaag aaatcatctg gaaagaaatc aagaagtgtg taaggaggct ctccttcgct
     5761 ccgtccatcc atgtgacgtg aatagttctc aaccccgttg gtatagccca tctcacgcag
     5821 catttcgata tcgtactctg tccgttgttt caagcgctgg gcttcaagca gtttgccttc
     5881 cttttcaaag acagctagtt gctcttctaa ttctgcttga atcttggcaa tagcaacttc
     5941 catgtggtcg tcattggtca caaagtgagt cgcagggaaa atcgctaaat gatccacttc
     6001 tccaaggact tgacctgtca aagcttcaac ctcacgaata cggtcaattt cgtctccaaa
     6061 aaattctact cgaaaagcgt gttcatcacg ggaagcaggg aaaatctcca ccacatcccc
     6121 acgaacgcga aatcttcccc gttggaaatc aatatcattt cgctcaaatt ggatatccac
     6181 caagtcattc aagagtttat cacgggaaat ttcaagacct ggacggagac taacgacact
     6241 atcagcgtat tccttgggcg aacccagacc atagatacaa gagaccgagg ccacgacaat
     6301 aacatcattg cgctccaata gggctgaagt cgctgagtga cgaagcttgt caatctcgtc
     6361 attgacagaa ctatccttct caatataggt atcactagaa gggacatagg cttctggctg
     6421 gtaatagtca tagtaggata cgaagtactc aactgcattt tcagggaaaa attccttgaa
     6481 ctccccatag agctgaccag ccagagtttt attgtgggca atgactagag tgggtttatt
     6541 gactttggaa atgacctgac tcatggtata ggtcttccct gttccggtcg cccccatcag
     6601 aatctgagct ttttctccac cctcgatatt atctaccaac tgctcgatag cttggggttg
     6661 atctcctgat ggttgatatt ttgatactag tttaaattta ttatctgtaa tgcggttaat
     6721 cataagaatc ctctctccat gtgctattcc tattttagca tacttgtatc attttcacga
     6781 agaaaaggac gaaccgaagt cacatccttt cattttcttt ctttaattgg taagcgagat
     6841 aaagaatcag cagaaacaaa ataatactgg acacgatgga accctcaata ccaaaagacc
     6901 caccagatag ccagtcttga ttgccttgtg gagtaaattt catgattgag gttcctgaag
     6961 actgaccact aactaatact ccaaagaggt tgccctgtgc gaaattccaa gctccatgaa
     7021 taccacctat accccatata gtatctgttt taagaagata tagggacatg gcaaccccaa
     7081 ataaaaagag atcaattgca gataagaaag ttatgcctgg atttcctaaa tgaagtaagg
     7141 taaagagact actagatatg ataatagcga ctttaagatt ggttctctta gccaattgtg
     7201 gaatcagcca agcacgagta accaattctt ctgccgttcc ttgtaaaatc caaaaaggta
     7261 ttgtaaataa agtaaaagct agcgaataag tatccaagtg aatggagtcc aaacgatatt
     7321 gccctaaggc caccaaacct aacaaggtca gaagaaaaag tgccagacct agactaaatc
     7381 ctttcagaag attgctgagg aaattctctc tataaaatcc caaggttcga ataggtcttt
     7441 tctcaacttt tcttgtccat ctgaacacag tgttaagaat aaaaccaaag gccagtaatc
     7501 ctaaaattaa acgaaagtca cttgttttat ccgtcaagct ctgatgcaag gtctgcaagt
     7561 agcttgcaag attttgacca gcctctccaa aatttctagc aagtccgatg agagctaaca
     7621 agccaatacc atacaaagta aaagccacaa actctcctat gaagacaaaa acaaaggcta
     7681 acaaggggct tgtgtaaatg tttaaactca tttttgaagc ttggaagtct ttaaatattc
     7741 ttttgttctt catatccccg tc
//
LOCUS       DAVHIT010000090         7731 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVHIT010000090 DAVHIT010000000
VERSION     DAVHIT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.092"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            213..2192
                     /locus_tag="VVN16_001790"
     CDS             213..2192
                     /locus_tag="VVN16_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5573118.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2259..3755
                     /locus_tag="VVN16_001791"
     CDS             2259..3755
                     /locus_tag="VVN16_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5573119.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3755..4681
                     /locus_tag="VVN16_001792"
     CDS             3755..4681
                     /locus_tag="VVN16_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5573120.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINVVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4690..5757
                     /locus_tag="VVN16_001793"
     CDS             4690..5757
                     /locus_tag="VVN16_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573121.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5768..6694
                     /locus_tag="VVN16_001794"
     CDS             5768..6694
                     /locus_tag="VVN16_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573122.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(6769..7731)
                     /locus_tag="VVN16_001795"
                     /pseudo
     CDS             complement(6769..7731)
                     /locus_tag="VVN16_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2302 a         1433 c         1612 g         2384 t
ORIGIN      
        1 tatgtcttcc taaactccaa agtttctgcg tcaaatttcc tattttcact gtagtctttg
       61 acacttcgaa cgacaatttg aatcatatat tcaaaaaaaa tgaaaaattt gtgccaacaa
      121 gcctaaatat tctgaaaatt tgtttgattt ttaatggata atgtgatata atggttgtca
      181 atctataatt cacataaaca gaggagtaga aaatgaaaaa aaatcgtgta tttgctacag
      241 caggtcttgt tttattagca gcaggtgtac ttgcagcatg cagttcttca aaatcatctg
      301 attcatcagc ccctaaagct tatggctatg tttatacagc agacccagaa accttggact
      361 acctgatttc aagtaaaaat agtacaacag tagtgacttc aaatgggatt gatggtttat
      421 tcactaacga taattacggt aatcttgctc ctgcagttgc agaggattgg gaagtctcta
      481 aggatggttt gacctacact tataagattc gtaaaggggt taaatggttt acctctgatg
      541 gagaagaata tgcagaggtg acggctaaag atttcgtgaa cggtttaaaa cacgcagcag
      601 ataaaaaatc agaagctatg tatttagctg aaaattcggt taaaggcttg gcagattatc
      661 tatcaggaac ttcaacagat ttttcaacag ttggtgtcaa ggcggttgat gattatacgt
      721 tacaatacac tttgaaccag cctgaaccgt tctggaactc taagttgacc tattctattt
      781 tctggcctct gaatgaagaa ttcgaaacat caaaaggaag cgattttgct aaaccaacag
      841 atccgacatc cttgctttat aatggtccat tcttgttgaa agggttgact gcaaaatctt
      901 ctgtagagtt tgtaaaaaat gagcaatatt gggataaaga aaatgtccac ctagatacta
      961 tcaatctagc ttactatgat ggatcagatc aggagtcgct agagcgtaac ttcactagtg
     1021 gagcttatag ttatgcccgt ctttacccta ccagctccaa ctattctaag gttgcagaag
     1081 aatacaagga caatatctat tacacacaat caggctctgg gattgctggt ctgggtgtga
     1141 atattgatcg ccaaagttac aactatactt ctaaaactac agattcagag aaagtagcta
     1201 ctaagaaggc attgcttaac aaagatttcc gtcaagcctt gaattttgct cttgatcgct
     1261 cagcttactc agctcaaatc aatggtaaag atggagcagc tttagcagtt cgtaatttat
     1321 ttgtaaaacc agactttgtt tcagctggtg agaagacctt tggtgattta gtcgctgctc
     1381 aacttcctgc ttatggtgat gagtggaaag gtgtgaattt agctgatggg caggatggtt
     1441 tattcaatgc tgacaaggcc aaggcagagt ttgcgaaagc taagaaagct ttagaagcag
     1501 acggcgttca gtttcctatt catctggacg ttccagtaga ccaagcatca aaaaactaca
     1561 tatctcgtat tcagtccttt aaacaatctg tagaaacagt tcttggtgtt gaaaatgtcg
     1621 ttgttgatat tcaacaaatg acaagtgatg aattccttaa tattacttac tatgctgcca
     1681 atgcttcatc tgaggattgg gatgtatcag gaggagtttc atgggggcca gactatcaag
     1741 acccatctac ttacctggat attttaaaaa caactagcag tgaaactaca aaaacatatt
     1801 taggatttga taatccaaat agcccttcag tagttcaagt tggtttgaaa gaatacgata
     1861 aattagttga tgaagctgcc agagagacaa gcgacttgaa tgtccgttat gaaaaatatg
     1921 cagcggctca agcatggttg acagatagtt cactctttat tcctgctatg gcttcttctg
     1981 gtgcagcacc agtgctttca cgaattgttc catttactgg agcttctgcg caaacaggct
     2041 ctaaggggtc agatgtttac ttcaaatatt tgaaattaca agataaagtg gtgactaagg
     2101 aagagtatga aaaagctcgt gaaaaatggt tgaaagaaaa agctgaatca aatgagaaag
     2161 ctcaaaaaga attggcaagt catgtgaagt aaataatttc taatagaact ttctctccat
     2221 gaggagaagg ttcttttggg atttttaaag gaaataatat gaaaaaatat atttttatgc
     2281 gtgttttgcg gtcattggtt tcgattttct tagtaacgac tttgacctac acgattatct
     2341 ataccttggt tcctcgaaaa ttgattttca agcaggatcc taactataat aaaattgcga
     2401 caacggctga taaacgtgat aactatgaaa atactgtgtt tgagcgtatg ggctacattg
     2461 agtattacga tactaaagag ttgcaagaaa aggcaagtag catggattct tctgtaacag
     2521 tagaagcaaa tgcgaccaat aaagctattt atgaaaagta catcaatcaa ttaggtcatg
     2581 gttggacttt gggagaattt actgaaagtg gtcaattcta tgctactcgt gaaattccaa
     2641 tttttgaacg tgtttttcac ttctatgcta acttgattga cattgatcat acaaataaaa
     2701 tccaagaccc tgaaaatcca gacttgaaac gctaccttcg ttttgaaaat gatccagcta
     2761 tcggatggtc attggtcggt tcaggaacta aacataaata tctcttgtac tttaacagtc
     2821 agttcccatt tgttcatcaa aactttgtga acttgaattt aggtgactct tacccaacct
     2881 atgctaatac accagttctt caggttatta ctcaaggtca aggacaaacc aaaactgccc
     2941 aagttcagtt cccaacaggt aagaaaacgt cttctgtaaa tatttactca agaacctaca
     3001 agtcacctag tcaggctgac tctcgtgaag tagctagcta tgggaaagat gatccttata
     3061 cagcgactga aagtaattac caatatccat ctatgattgt cagctctgct attactggtt
     3121 tgattggttt ggttcttgcc tatgctcttg ccgtgccact tggttcagcc atggctcgtt
     3181 tcaagaacac ttggattgat agcctctcaa cagggacttt gaccttcttg cttgctcttc
     3241 caacgattgc cttggtttac atcgttcgat tgattggatc atctattgcc cttccagatt
     3301 cattccctat cttgggagct ggagattggc gttcttacgt tttaccagca gtcatccttg
     3361 gtttgttggg tgctcctgtt acagccattt ggattcgtcg ttacatgatt gacttgcaat
     3421 ctcaagactt tgttcgtttc gctcgtgcaa aaggtttgtc tgaaaaagaa atttcaaaca
     3481 aacacatctt taaaaatgcc atggttccgc tggtttcagg aattcctggt gccattattg
     3541 gggttatcgg tggtgcaacc cttactgaaa cagtcttcgc cttcccaggt atgggtaaaa
     3601 tgttgattga ctctgtaaaa gcatctaata actctatggt cgttggtctt gtcttcatct
     3661 ttacatgtat ttctatcttc tcacgtcttt tgggagatat ttggatgact attattgacc
     3721 cacgtattaa attgactgag aaaggaggca aataatgtct acaatcgata aagaaaaatt
     3781 tcagtttgta aaacgtgacg attttgcctc tgaaactatt gatgcgccag catattctta
     3841 ctggaaatca gtgtttaaac aatttatgaa gaaaaaatca actgtagtca tgttgggaat
     3901 cttggtagcc atcattttga taagtttcat ctacccaatg ttttctaagt ttgatttcaa
     3961 tgatgtcagc aaggtaaacg actttagtgt tcgttatatc aagccaaatg cggagcattg
     4021 gttcggtact gacagtaacg gtaaatcgct ctttgacggt gtctggttcg gagctcgtaa
     4081 ctccatcctc atttctgtga ttgcgacagt gattaacgtg gttatcggtg tttttgtcgg
     4141 tggtatttgg ggtatttcaa aatcagttga ccgtgtcatg atggaagttt acaacgtcat
     4201 ctcaaacatc ccacctcttt tgattgttat tgtcttgact tactcaatcg gagctggatt
     4261 ctggaatctg atttttgcca tgagcgtaac aacatggatt ggtattgcct tcatgatccg
     4321 tgtgcaaatc ttgcgctatc gtgacttgga atacaacttg gcgtcacgta ctttgggaac
     4381 accaaccttg aagattgttg ccaaaaatat catgcctcaa ttggtatctg ttattgtgac
     4441 aaccatgact caaatgcttc caagctttat ctcatacgaa gccttcttgt ctttcttcgg
     4501 tcttggatta ccgattacag tgccaagttt gggtcgtttg atttcggatt attcacaaaa
     4561 cgtaacaacc aatgcttact tgttctggat tccattgaca acccttgtct tggtatcctt
     4621 gtcccttttc gtagttggtc aaaacttagc ggatgctagt gatccacgta cacatagata
     4681 ggagtagaaa tgacaaaaga aaaaaatgta attttgactg ctcgcgatat tgtcgtggaa
     4741 tttgacgttc gtgacaaagt attgacagcc attcgcggcg tttcccttga actagtcgaa
     4801 ggagaagtat tagccttggt aggtgagtca ggatcaggta aatctgtttt gacaaagacc
     4861 ttcacaggta tgctcgaaga aaatggtcgt attgcccaag gtagtattga ctaccgtggt
     4921 caggacttga cagctttatc ttctcacaag gattgggaac aaattcgtgg tgctaagatt
     4981 gcgactatct tccaggaccc aatgactagt ttggacccca ttaaaacaat tggtagtcag
     5041 attacagaag ttattgtaaa acaccaagga aaaacagcta aagaagcgaa agaattggcc
     5101 attgactaca tgaataaggt tggcattcca gacgcagata gacgttttaa tgaataccca
     5161 ttccaatatt ctggaggaat gcgtcaacgt atcgttattg cgattgccct tgcctgccga
     5221 cctgatgtct tgatctgtga tgagccaaca actgccttgg atgtaactat tcaagctcag
     5281 attattgatt tgctaaaatc tttacaaaac gagtatcatt tcacaacaat ctttattacc
     5341 cacgaccttg gtgtggtggc aagtattgcg gataaggtag cggttatgta tgcaggagaa
     5401 atcgttgagt atggaacggt tgaggaagtc ttctatgacc cttgccatcc atatacatgg
     5461 agtctcttgt ctagcttgcc tcagcttgct gatgataaag gggatcttta ctcaatccca
     5521 ggaacacctc cgtcacttta tactgacctg aaaggggatg cttttgcctt gcgttctgac
     5581 tacgcaatgc agattgactt cgaacaaaaa gctcctcaat tctcagtatc agagacacat
     5641 tgggctaaaa cttggcttct tcatgaggat gctccaaaag tagaaaaacc agctgtgatt
     5701 gcaaatctcc atgataagat ccgtgaaaaa atgggatttg cccatctggc tgactaggag
     5761 gaaggaaatg tctgaaaaat tagtagaaat taaagattta gaaatttcct tcggtgaagg
     5821 aagtaagaag tttgtcgcgg ttaaaaatgc taacttcttt atcaacaagg gagaaacttt
     5881 ctcgcttgta ggtgagtccg gtagtgggaa aacaactatt ggtcgtgcta tcatcggtct
     5941 aaatgataca agtaatggag atatcatttt tgatggtcaa aagattaatg gtaagaaatc
     6001 gcgtgaacaa gctgcggaat tgattcgtcg aatccagatg attttccaag accctgccgc
     6061 aagtttgaat gaacgtgcga ctgttgatta tattatttct gaaggtcttt acaatcaccg
     6121 tttatttaag gatgaagaag aacgtaaaga gaaagttcaa aatattatcc gtgaagtagg
     6181 tcttcttgct gagcacttga ctcgttaccc tcatgaattc tcaggcggtc aacgtcaacg
     6241 tatcggtatt gcccgtgcct tggtcatgca accagacttt gttattgcag atgagccaat
     6301 ttcagccttg gacgtttctg tacgtgccca agtcttgaac ttgctcaaaa aattccaaaa
     6361 agagctcggc ctgacctatc tcttcatcgc ccatgacttg tcggttgttc gctttatttc
     6421 agatcgtatc gcagttattt acaagggtgt tattgtagag gttgcagaaa cagaagaatt
     6481 gtttaacaat ccaattcacc catatactca agccttgctt tcagcggtac caatcccaga
     6541 tccaatcttg gaacgtaaga aggtcttgaa ggtttacgat ccaagtcaac acgactatga
     6601 gactgataag ccatctatgg tagaaatccg tccaggtcac tatgtttggg cgaaccaagc
     6661 cgaattggca cgttatcaaa aaggactaaa ctagtaatgg ttttataatt tccatgtcaa
     6721 tttttatgga aattaggctc tttgtcaact gtagtgggtt gaagtcagct aagctcgaga
     6781 aaggacaaat ttcgtccttt cttttttgat gttcaaagcg ataaaaatcc gttttttgaa
     6841 gttttcaaag tttcgaaaac caaaggcatt gcgcttgata agtttgatga gattattggt
     6901 cgcttccagt ttggcattag aatagtgtag ttgaagggcg ttgataatct tttctttatc
     6961 tttgaggaag gttttaaaga cagtctgaaa aataggatga acctgcttaa gattgtcctc
     7021 gataagttcg aaaaatttct ctggttcctt attctggaag tgaaaaagca agagctgata
     7081 gagctgatag tggtgtttca agtcttccga ataactcaaa agcttgttta aaatctcttt
     7141 attggttaag tgcatacgaa aagtaggacg ataaaaacgt ttatcactca gtttacggct
     7201 atcctgttgt atgagcttcc agtagtgctt gatagccttg tattcatggg attttcgatg
     7261 aaactgattc atgatttgga cacgcacacg actcatagca cggctaagat gttgtacaat
     7321 gtgaaagcga tcaagaacga ttttagcatt cgggagtgaa acagtctagg agactgtttc
     7381 agcctgagcc tagaaatttg aaagcgaagc tgtttagcca agtcatagta agggctaaac
     7441 atatccatag taatgatttt gacgcgacat cggacggctc tatcatattt aagaaagtga
     7501 tctcggatga cagcttgtgt tctaccttca agaacagtga tgatatcgag cttttcaaaa
     7561 tcttgcgcaa taaagctcat ctccatctcc cgattgaaac agtcactccc cggactgttt
     7621 caacgtccca agacataatc ttaggcagat ttctaaaatt acactcaaag tggaagtcat
     7681 tgagctttca aatgacagtt gaagttgaga tggaaagctg atgggcaata t
//
LOCUS       DAVHIT010000091         7637 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVHIT010000091 DAVHIT010000000
VERSION     DAVHIT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.093"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            147..446
                     /locus_tag="VVN16_001796"
     CDS             147..446
                     /locus_tag="VVN16_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU5573123.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWNGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            517..2538
                     /locus_tag="VVN16_001797"
     CDS             517..2538
                     /locus_tag="VVN16_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5573124.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFIYSTYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDIENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNILYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2540..3181
                     /locus_tag="VVN16_001798"
     CDS             2540..3181
                     /locus_tag="VVN16_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573125.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3285..4637
                     /locus_tag="VVN16_001799"
     CDS             3285..4637
                     /locus_tag="VVN16_001799"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5573126.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4641..5699
                     /locus_tag="VVN16_001800"
     CDS             4641..5699
                     /locus_tag="VVN16_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU5573127.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5709..6863
                     /gene="divIB"
                     /locus_tag="VVN16_001801"
     CDS             5709..6863
                     /gene="divIB"
                     /locus_tag="VVN16_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU5573128.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEAALAEEKE
                     KERQTRMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEESESKEKE
                     EQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKDIRVE
                     GTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTKFTIK
                     VKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFVSELA
                     QISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKPQLSE
                     PSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESN"
BASE COUNT         2349 a         1318 c         1672 g         2298 t
ORIGIN      
        1 tctaaaaaat tcgatttttt ccttgacaac aaataataaa aatgttattc ttatcacata
       61 agaaagcggt ttcaacaaaa gagagaggag gatttatttt gttgcattgt tgctcagggt
      121 gtgctttttt tgaaagaaaa aataaaatga aaacgaaaaa acatagatta cttgctctag
      181 ctcttatttc aagttttaca ttattgggag ctgcatcagc tgctgtacaa tatccagatg
      241 gaggagtatg gacatatgga gaaggttcag gaggtggttg ggctttttca aattactatc
      301 atggtaaaaa atatcattat tcatccatcg tgagcagatg gaatggtcat tctgataaag
      361 gagaagctcc tgctggaaaa acctcctatg catggatttg gactaaatgg ggagaacaag
      421 tagcatttta ctgtgattat gactaatcgt tcattgattt aaatcgttcc ccttgtaaga
      481 aattacaggg ggaattttgt ttacaaggag tctactatga agaaactatt catattatta
      541 tcaacttttt ttctcagctt cttccttgct tggattattg tcttacgtgc gccacaatat
      601 ttatatgcaa gctatgattc cgtttcttta cttcgtgtca aaaaagatac tcaggaaccg
      661 acgcgtgagg tatttgaaca ggaattggag aattttgcaa attcagaaca gagtttaata
      721 gctagaagaa ttgtagagcc gagtaaggat gggacgactc actttattta ttcaacttat
      781 ggtcagggaa ctttaccaaa agaattccaa gaagctagcc aagaaagtcg tgaacgtagt
      841 gatccgctaa atagttatct ccttttgtca ggttccttga cgaaagaaaa gcttgccgat
      901 aaattaggag atttgggtta taaagcaagt gctgaccgaa agataccgcc ctattttctt
      961 gcttttcgaa tattactaaa tccccttatt ttaattagtt tagcaatatt tggcttatct
     1021 ttctttgctt tagtgattat cactcggatt aaggaaatga gagcagcagg tataaaactc
     1081 ttttctggtc agactctctt atccatcatg gggcattctt tatctactga tatcaaatgg
     1141 ctccttctat cagccctcct ttccttccta ggtgggggag tcgttctttt tagtcaaggt
     1201 ttgttttatc ctatcttgtt agccacctat ggttttggga ttagtttcta tctgttgttt
     1261 ttattggcga tttcaatttt actaatgctc ctttatctaa tgagtttgaa ttacaaagca
     1321 ttagttcccg ttattaaggg gagattaccc cttaaacgcc tgatgatttt aaccctattg
     1381 tgtcagttgg ttgctgtctt tacagtagga tacgctgtta agacaggttt gacgtcttac
     1441 caacgattga aagaacttga aatttcaaaa caagcatggc aggatagagc agactattat
     1501 caaatttctt ttggcttagg tgatagagga aaagatatag aaaatcagag caagtggtat
     1561 gcctttgcca aggaagcaat cgaagaagaa caagctcttt atgtaaagga taatctgctc
     1621 cattttgcca atccacaagg aaaaaatgaa cagggagaga cactggatac ctatagtcca
     1681 gatgctaata cgctctatgt tagtcccagt tatttggaca aggaaaaggt cgtggtagat
     1741 gctgagacca aacagaagtt agcccatctc caaaaaggtg agtttatcct cttgctccca
     1801 gaacatttgc gctctcgaga agcagaactt aagaaagttt ttgaagaaag attgagttat
     1861 tatggaaaat ctggtgagga ggcaagtgct cctttggatt atgagatgaa agcgcacgtt
     1921 agttatcttt caatgggaga aaagcggttt gtttataata acggtgagaa tcccgtatcc
     1981 actcagtatt tgactgatcc gattttagtg gtattcacgc cgacttctac aggtgatagt
     2041 tttatatcct tatctagttg gtctatcaat gctggaaaac aactctttat caaaggatat
     2101 gagagtgggc tagaactctt gaagaaagct ggaatttatg agcaagtatc ctatcttaaa
     2161 gaaggaagaa gtgtttatct aactcgttat aatgaagttc aaactgaaac agcaacttta
     2221 atcttaggag ctattgtggg gatagctagt tccttgttac tattttattc tgtcaatatt
     2281 ctatatttcg agcaattccg ccgagatatc ttgattaaac gaatttcagg tttacgattt
     2341 tttgaaacac atgctcagta tatggttagc caatttgcca gttttgtatt tggtgctagt
     2401 ctctttattt taagcagtcg agacttggtg attggcttgc tcactttatt ggtctttcta
     2461 gctagtgcag ttttgacgct ttaccgtcaa gcgcagaaag aatctcgtgt ttctatgaca
     2521 attatgaaag gaaaatagga tgattgaact aaagaatata tctaaaaaat ttggaagccg
     2581 tcagctattt tcagatacga atcttcattt tgaaggtggg aaaatttatg ccttaatcgg
     2641 tacaagtggc tgtggtaaga caacactctt gaatatgatt ggacgattgg agccatatga
     2701 caaagggcaa atcatctatg atggcacttc tcttaaggac atcaagcctt ctgttttctt
     2761 tagagattac ttgggatact tatttcaaga ttttggctta attgaaagcc aaaccgtcaa
     2821 agagaatctc aatctgggtt tagttggtaa aaagttgaag gaaaaagaga aaatctcttt
     2881 gatgaaacaa gctctaaacc gtgtaaacct ctcttatttg gatttgaagc aacctatatt
     2941 tgagttatca ggaggagaag cacaacgtgt tgcactagcg aagataattt taaaggatcc
     3001 gcctttgatt cttgcagatg aaccaaccgc ttccttagac cccaaaaact ctgaggaatt
     3061 actttccatc ctagaatctt taaaaaatcc gaatcggacc attattattg cgacccacaa
     3121 tcctctgatt tgggagcaag tggatcaggt cattcgagtt accgatttat cacatagatg
     3181 atatggtaag attcagttag aagaaagagt cacaaacaca ctttgtggct tttttatttc
     3241 cataaaaatg gtaaaatagt aggagtagaa atggagttcg agacatgaaa gtaatagatc
     3301 aatttaaaaa taagaaagtt cttgttttag gtttggccaa gtctggtgaa tctgcggctc
     3361 gtttgttgga caagttaggt gccattgtga cagtaaatga tgggaaacca ttcgaggaca
     3421 atccagctgc ccaatgtttg ctggaagaag ggatcaaggt cattacaggt ggccatcctt
     3481 tggaactctt ggatgaagag tttgccctca tggtaaaaaa tccaggtatc ccctacagca
     3541 atcccatgat tgaaaaggct ttggccaagg gaattccagt cttgactgag gtggaattgg
     3601 cttatttgat ttcagaagca ccgattattg gtatcacagg atcgaacggt aagacaacca
     3661 caacgactat gattggggaa gttttgactg ctgctgggca acatggtctt ttatcaggga
     3721 atatcggcta tcctgccagt caggttgctc aaatagcatc agataaggac acgcttgtta
     3781 tggaactttc ttctttccaa ctcatgggtg ttcaagaatt ccatccagag attgcggtta
     3841 ttaccaacct catgccaact catatcgatt atcatggatt atttgaggaa tatgtagcag
     3901 ccaagtggaa tatccagaac aagatgacag cagctgattt ccttgtcttg aactttaatc
     3961 aagacttggt aaaagacttg gcttccaaga cagaagccac tgttgtacca ttttcaacac
     4021 ttgaaaaggt tgatggagct tatctggaag atggtcaact ctacttccgt ggtgaagtag
     4081 tcatggcagc gaatgaaatc ggtgttccag gtagccacaa tgtggaaaat gcccttgcga
     4141 ctattgctgt agccaagctt cgtggtgtgg acaatcaaac catcaaggaa actctttcag
     4201 ccttcggtgg tgtcaaacac cgtctccagt ttgtggatga catcaagggt gttaaattct
     4261 ataacgacag taaatcaact aatatcttgg ctactcaaaa agccttgtca ggatttgaca
     4321 acagcaaggt cgtcttgatt gcaggtggtt tggaccgtgg caatgagttt gacgaattgg
     4381 tgccagatat tactggactc aagaagatgg tcatcctggg tcaatctgca gaacgtgtca
     4441 aacgggctgc agacaaggct ggtgtcgctt atgtggaggc gacagatatt gcagatgcga
     4501 cccgcaaggc ctatgagctt gcgactcaag gagatgtggt tcttcttagt cctgccaatg
     4561 ccagctggga tatgtatgct aactttgaag tacgtggcga cctctttatc gacacagtag
     4621 cggagttaaa agaataaaat atgaaaaaaa ttgtctttac aggtgggggg acggttggac
     4681 acgttaccct caatcttttg ttaatgccca agttcatcga agatggttgg gaagtccact
     4741 atatcgggga caagcgtggt atcgaacacc aagaaatcct taagtcaggt ttggatgtca
     4801 ctttccattc tattgcgact ggaaaattgc gtcgctattt ctcttggcaa aatatgctgg
     4861 acgtcttcaa agttggctgg ggaatcgtcc aatcgctctt tatcatgttg cgactgcgtc
     4921 cacagaccct tttttcaaag gggggctttg tctcagtacc gcctgttatc gctgcgcgtg
     4981 tgtcaggagt gcctgtcttt attcacgaat ctgacctgtc tatgggcttg gccaataaaa
     5041 tcgcttataa atttgcgact aagatgtatt caacctttga gcaagcttcg agtttggcta
     5101 aggtcgagca tgtgggagcg gtgaccaagg tttcggacaa aaacactcca gaaccagatg
     5161 aattggtgga tattcaaacc cactttaatc ccaaattgcc aactgtattg tttgtcggcg
     5221 gttctgcagg cgctcgtgtc tttaaccaat tggtgacaga ccataagaaa gaactaacag
     5281 agcgctacaa tattatcaat ctaactggag attctagcct gaatgagttg agccaaaatc
     5341 tttttcgtgt tgactatgtg accgatctct atcaaccctt gatggaattg gctgatattg
     5401 ttgtgacacg tggtggtgcc aatacgattt ttgagctctt ggcgatagca aaattgcatg
     5461 tcattgtgcc acttggtcgt gaagctagtc gtggtgacca gattgaaaat gcagcttact
     5521 ttgttaagaa aggctatgca gaagaccttc aagaaagcga tttgaccttg gatagtttgg
     5581 aagagaagct ttctcactta ctaagtcaca aggaagatta ccaagctaag atgaaagctt
     5641 ctaaggaatt gaaatctcta gcagattttt atcaattgtt gaaaaaagat ttatcataag
     5701 gaaagtaaat gtcaaaagat aagaaaaatg aggacaaaga aatcctcgaa gaattgaaag
     5761 agttatcaga atggcagaaa cgaaaccaag aatatctaaa aaagaaggct gaagaagcgg
     5821 ctctagctga ggagaaggaa aaggaaagac aaactcggat gggagaagaa tctgagaagt
     5881 cagaggacaa acaggaccag gagagtgaaa cagaccagga agattcagaa tcagctaagg
     5941 aagagtctga agaaaaagta gcatcctcag aggctgacaa agagaaagaa gaatcagagt
     6001 ctaaagagaa ggaggaacag gataaaaagc ttgctaaaaa ggctacaaag gaaaaaccag
     6061 ccaaagcaaa gattcctggt atccatatct tgcgagcctt cacgatttta tttccaagtc
     6121 tgcttttatt gattgtctct gcctacttgc tcagtcctta tgcgaccatg aaagatattc
     6181 gtgttgaggg aacggtgcaa actacagctg atgatattcg acaggcttca ggcattcagg
     6241 attcggatta tacgattaac cttctgctag acaaggcaaa atatgaaaag cagattaagt
     6301 ctaactattg ggttgaatca gctcaacttg tctatcaatt tccaactaag ttcactatta
     6361 aggtcaagga atatgatatt gtggcctact atatttctgg tgaaaatcat tatcctattc
     6421 tttccagtgg tcagcttgag actagttctg tgagtctgaa cagtttacca gaaacttatt
     6481 tatcagttct ctttaatgat agtgaacaaa tcaaggtttt tgtctcagaa cttgctcaaa
     6541 ttagcccaga actcaaggcg gctatccaaa aggtggaatt agccccaagc aaggtgacat
     6601 ccgatttaat tcgattgacc atgaatgatt cggacgaagt cttggttcct ctatctgaaa
     6661 tgagtaagaa actgccatat tacagtaaga ttaagccaca attgtcagaa ccgagtgtga
     6721 ttgatatgga agctggaatt tacagttaca ctgtggcgga taaattaatt atggaggctg
     6781 aggaaaaagc caaacaagag gccaaggaag ctgagaaaaa acaggaagaa gaacagaaaa
     6841 aacaagagga agagagcaat tgaaatcaaa cgacccagcg ctcatcgcgt cgctaggttt
     6901 accttttctc ttatagttct ttagtgacca tgttcttacg tttaatattt gacatctgtt
     6961 tttctttatg ttacatttgc aatgtaatcg attacaaaat aattttttat gaagaaggta
     7021 acacatatgt cagatgaagc attcttattt gaagaaattg aagaaatcgt agcctctaca
     7081 gatggtgaat ttttggggga gttttacttg gaactggggt agttctctta attggaatag
     7141 cctgttgtta aaaagatagg gagtgataat catgcaagat aactttttat ttgaggaaat
     7201 tgaagaaatt tcagtaccag ttagtgattt ttcagctgga cttgcaacag gtatcggatt
     7261 tggtttagca atccttgctc ttgctggttg ttgaagtttg ttcatttact aacatcaagc
     7321 tttttcaatt tcattttaga cagtcatttg aattttccgt attagtcttg cagcaagaga
     7381 ttaatagaat tagtcattat tttattgatt gcggactgag ggactagagt atgttttact
     7441 tagcccctct tttatttatt aaaggttagg tttgttatga gaattgttga taagattaag
     7501 atattaccta ctccttatga gggacagtat catctatata taccatccag taagaaacat
     7561 gtattagttg ggaaacagga aaaaaatggt tagagcaact aataggtcaa gaatttacca
     7621 tatcggactt attagtg
//
LOCUS       DAVHIT010000092         7565 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVHIT010000092 DAVHIT010000000
VERSION     DAVHIT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.094"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            110..310
                     /locus_tag="VVN16_001802"
     CDS             110..310
                     /locus_tag="VVN16_001802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU5573129.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            345..1061
                     /gene="atpB"
                     /locus_tag="VVN16_001803"
     CDS             345..1061
                     /gene="atpB"
                     /locus_tag="VVN16_001803"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU5573130.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1075..1569
                     /gene="atpF"
                     /locus_tag="VVN16_001804"
     CDS             1075..1569
                     /gene="atpF"
                     /locus_tag="VVN16_001804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU5573131.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            1569..2105
                     /locus_tag="VVN16_001805"
     CDS             1569..2105
                     /locus_tag="VVN16_001805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU5573132.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2120..3625
                     /locus_tag="VVN16_001806"
     CDS             2120..3625
                     /locus_tag="VVN16_001806"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5573133.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            3641..4519
                     /locus_tag="VVN16_001807"
     CDS             3641..4519
                     /locus_tag="VVN16_001807"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU5573134.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            4605..6011
                     /gene="atpD"
                     /locus_tag="VVN16_001808"
     CDS             4605..6011
                     /gene="atpD"
                     /locus_tag="VVN16_001808"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5573135.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTISMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6022..6441
                     /locus_tag="VVN16_001809"
     CDS             6022..6441
                     /locus_tag="VVN16_001809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU5573136.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(6486..7499)
                     /locus_tag="VVN16_001810"
     CDS             complement(6486..7499)
                     /locus_tag="VVN16_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU5573137.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         2244 a         1418 c         1677 g         2226 t
ORIGIN      
        1 aaatggaaat aatttttatt aaacgcttcc aatagcggtt aaaagttgac aaatttaaat
       61 tttaggacta cactaagtaa gtaaatttta ttttaaagga gaatttgtta tgaatttaac
      121 atttttaggc ttatgtattg cctgtatggg cgtatctgtc ggtgaaggtt tattgatgaa
      181 tggactgttt aaatcagtag cacgccaacc agatatgctt tctgagtttc gtagtttgat
      241 gtttttaggt gttgccttta ttgaaggaac tttctttgta actcttgtct tctcatttat
      301 tatcaaataa atacatggaa cgagaagaaa agggaggatt ttagatggaa gaaagtatta
      361 atccaatcat ctctattggt cctgttatct tcaatctgac tatgttagcc atgactttgt
      421 tgattgtggg agttattttt gtctttattt attgggcaag ccgcaatatg accttgaaac
      481 ccaaaggaaa gcaaaatgta cttgagtatg tctatgactt tgttattgga tttacagaac
      541 ctaacattgg ttcgcgctac atgaaagatt actcactctt tttcctttgt ttattccttt
      601 tcatggtgat tgccaataac cttggcttaa tgacaaagct tcaaacgatc gatgggacta
      661 actggtggag ttcgccaacc gctaatttac agtatgactt aaccttatct tttcttgtca
      721 ttttgttgac acatatagaa agcgttcgtc gtcgtggatt taaaaaaagt ataaaatctt
      781 ttatgagtcc tgtttttgtc ataccgatga atatcttgga agaatttaca aacttcttat
      841 ctttggcttt gcggattttt gggaatatct ttgcaggaga ggtcatgacg agtttgttac
      901 ttcttctttc ccaccaagct atttattggt atccagtagc ctttggagct aatttggctt
      961 ggactgcatt ttctgtcttt atttcctgca tccaagctta tgtttttact cttttgacat
     1021 ctgtgtattt agggaataag attaatattg aagaggaata gaaaggagta actgatgcac
     1081 gtaacagtag gtgaattaat tggtaatttt attttaatca ctggctcttt tattcttttg
     1141 ctagtcttga ttaaaaaatt tgcatggtct aatattacag gcattttcga agaaagagct
     1201 gaaaaaattg cttcagatat tgacagagct gaagaagccc gtcaaaaagc agaagtattg
     1261 gctcaaaaac gcgaagatga attggctggt agccgtaaag aagctaagac aatcattgaa
     1321 aatgcaaagg aaacagctga gcaaagtaag gctaatatct tagcagatgc taaactagaa
     1381 gcaggacact taaaagaaaa agccaatcaa gaaattgctc aaaataaagt agaagcttta
     1441 cagagtgtta agggtgaggt cgcagatttg accatcagct tagctggtaa aatcatctca
     1501 caaaaccttg acagtcatgc ccataaagca ctcattgatc agtatatcga tcagctagga
     1561 gaagcttaat ggacaagaaa acagtaaagg taattgaaaa atacagcatg ccttttgtcc
     1621 aattggtact tgaaaaagga gaagaagacc gtatcttttc agacttgact caaatcaagc
     1681 aagttgttga aaaaacaggt ctgccttctt ttttaaaaca agtggcagta gacgagtcgg
     1741 ataaggaaaa aacaattgct tttttccaag attctgtgtc acctttatta caaaacttta
     1801 tccaggttct ggcctacaat cacagagcaa atctttttta tgatgtgctt gtagattgct
     1861 tgaaccgact tgaaaaagaa acaaatcgat ttgaagtgac gattacgtct gctcatcctc
     1921 taactgatga acagaagact cgtttgctcc ctttgattga gaaaaaaatg tctctgaaag
     1981 taaggagtgt aaaagaacaa atcgatgaaa gtctcattgg tggttttgtc atttttgcca
     2041 atcacaagac aattgatgtg agtattaaac aacaacttaa agttgttaaa gaaaatttga
     2101 aatagaaagt ggtgttcttt tggcaattaa cgcacaagaa atcagcgctt taattaagca
     2161 acaaattgaa aatttcaaac ccaattttga tgtgactgaa acaggtgttg taacctatat
     2221 cggggacggt atcgcgcgtg ctcatggcct tgaaaatgtc atgagtggag agttgttgaa
     2281 ttttgaaaac ggctcttatg gtatggctca aaacttggag tcaacagacg ttggtattat
     2341 catcctaggt gactttacag atatccgtga aggcgataca atccgccgta cagggaaaat
     2401 catggaagtc cctgtaggtg aaagtctgat tggtcgtgtt gtggatccgc ttggtcgtcc
     2461 agttgacggt cttggagaaa tccacactga taaaactcgt ccagtagaag caccagctcc
     2521 tggtgttatg caacgtaagt ctgtttcaga accattgcaa actggtttga aagctattga
     2581 cgcccttgta ccgattggtc gtggtcaacg tgagttgatt atcggtgacc gtcagacagg
     2641 gaaaacaacc attgcgattg atacaatctt gaaccaaaaa gatcaagata tgatctgtat
     2701 ctacgtcgcg attggacaaa aagaatcaac agttcgtacg caagtagaaa cacttcgtca
     2761 gtacggtgcc ttggactaca caatcgttgt gacagcctct gcttcacaac catctccatt
     2821 gctcttccta gctccttatg ctggggttgc tatggcggaa gaatttatgt atcaaggtaa
     2881 gcatgttttg attgtatacg atgatctatc aaaacaagcg gtagcttatc gtgaactgtc
     2941 gctcttgctt cgtcgtcctc caggtcgtga agccttccca ggggatgttt tctatctcca
     3001 cagccgtttg cttgagcgct cagctaaagt ttctgatgaa cttggtggtg gatcaattac
     3061 agccctacca tttatcgaga cacaagcagg agatatctca gcctatatcg caaccaacgt
     3121 gatttctatc actgatggac aaatcttcct tggcgatggc ctcttcaatg caggtattcg
     3181 tccagccatc gatgcgggtt catctgtatc tcgtgtaggt ggttctgcac aaatcaaagc
     3241 catgaagaag gttgctggta cacttcgtat cgaccttgct tcataccgtg agttggaagc
     3301 ctttactaag tttggttctg acttggacgc agcaacacag gctaagttga accgtggacg
     3361 tcgtaccgtt gaggtcttga aacaacctgt tcacaaacca ttacctgttg agaaacaagt
     3421 aaccattctt tatgctttga cacatggttt cttggatact gttccagtag atgatattgt
     3481 tcgtttcgag gaagagttcc atgccttctt tgatgctcaa catccagaga ttttggaaac
     3541 cattcgtgat acaaaagact tgccagaaga agcagtcttg gatgctgcga ttacagagtt
     3601 tctcaatcaa tctagcttcc aataagaata gaggtgtcag atggcagtgt ctctaaatga
     3661 tattaaaaca aaaatcgcct caacaaaaaa tacgagtcaa atcactaatg ctatgcaaat
     3721 ggtatcggct gctaagttag gtcgctctga agaagctgct cgcaacttcc aagtttacgc
     3781 tcagaaagtg cgtaaacttt tgacagatat ccttcatggt aatggagctg gtgcttcaac
     3841 taatccgatg ttgattagcc gttctgtgaa gaagacaggc tatatcgtta tcacttcaga
     3901 ccgcggtttg gttggaggtt ataattcctc tattttgaaa gctgttatgg agttgaaaga
     3961 agaataccac ccagacggta aaggttttga aatgatctgt atcggtggga tgggagctga
     4021 tttctttaag gctcgcggta ttcaaccact ttatgaatta cgtggcttgg cagaccaacc
     4081 tagctttgat caagttcgta agattatttc aaaaactgtt gaaatgtacc aaaatgaact
     4141 ctttgatgag ctttatgttt gctacaacca ccatgtcaat acgctaacca gtcaaatgcg
     4201 tgtggaacaa atgcttccga ttgttgactt ggatccaaat gaggcggatg aagagtacag
     4261 cttgactttt gaattggaaa ccagccgaga agaaattctg gagcagttgt tgcctcagtt
     4321 tgcagaaagt atgatttacg gtgccattat cgatgccaag acagctgaga atgctgcggg
     4381 tatgacagcc atgcaaacag cgacagataa tgctaagaaa gtcatcaatg atttgacaat
     4441 tcagtataac cgtgccagac aggcggcgat tacacaagaa attacagaaa tcgtagcagg
     4501 tgctagtgcc ttagaatagg ctctagtcca gctcgtatga aaatgaactt aggacctagt
     4561 tgagctagga accgacagta tcttatatag aataggagaa ggagatgagt tcaggtaaaa
     4621 ttgctcaggt tatcggtccc gttgtagacg ttttgtttgc agcaggggaa aaacttcctg
     4681 agattaacaa tgcacttgtc gtctacaaaa atgacgaaag aaaaacaaaa atcgtccttg
     4741 aagtagcctt ggagttagga gatggtatgg ttcgtactat ctccatggaa tcaacagatg
     4801 ggttgactcg tggaatggaa gtattggaca caggtcgtcc aatctctgta ccagtaggta
     4861 aagaaacttt gggacgtgtc ttcaacgttt tgggagatac cattgatttg gaagctcctt
     4921 ttacagaaga cgcagagcgt cagccaattc ataaaaaagc tccaactttt gatgagttgt
     4981 ctacctcttc tgaaatcctt gaaacaggga tcaaggttat tgaccttctt gccccttacc
     5041 ttaaaggtgg taaagttgga cttttcggtg gtgccggagt tggtaaaacc gtcttaatcc
     5101 aagaattgat tcacaacatt gcccaagagc acggtggtat ttcagtattt actggtgttg
     5161 gggaacgtac tcgtgagggg aatgaccttt actgggaaat gaaagaatca ggcgttatcg
     5221 agaaaacagc catggtcttt ggtcagatga atgagccacc aggagcacgt atgcgtgttg
     5281 cccttactgg tttgacaatc gctgaatact tccgtgatgt ggaaggccaa gacgtgcttc
     5341 tctttatcga taatatcttc cgtttcactc aggctggttc agaagtatct gcccttttgg
     5401 gtcgtatgcc atcagccgtt ggttaccaac caacacttgc tacggaaatg ggtcaattgc
     5461 aagaacgtat cacatcaacc aagaagggtt ctgtaacctc tatccaggct atctatgtgc
     5521 cagcggatga ctatactgac ccagcgccag caacagcctt cgctcacttg gattcaacaa
     5581 caaacttgga acgtaaattg gtacaattgg gtatctaccc agccgttgac ccacttgctt
     5641 caagctcacg tgccttggca cctgaaatcg ttggagaaga gcactatgca gttgctgctg
     5701 aagtaaaacg tgtccttcaa cgttaccatg aattgcaaga tatcattgct atccttggta
     5761 tggatgagct ttctgatgaa gaaaagacct tggttgctcg cgcccgtcgt atccagttct
     5821 tcttgtcaca aaacttcaac gttgcggaac aatttactgg tcagccaggt tcttatgttc
     5881 cagttgctga aactgtacgt ggctttaagg aaatccttga tggtaaatac gaccacttgc
     5941 cagaagatgc cttccgtggt gtaggttcta tcgaagatgt gattgcaaaa gctgaaaaaa
     6001 tgggatttta agaggtgacc tatggctcag ttaactgtcc agatcgtgac accagatggt
     6061 ctcgtctatg atcaccatgc cagctatgta tcggttcgaa ctctggatgg tgagatgggg
     6121 atcttgccac gacatgaaaa tatgattgcg gttttagcag ttgatgaagt aaaggtaaaa
     6181 cgtatcgatg ataaagatca cgtgaactgg attgcagtaa acggtggcgt tattgaaatt
     6241 gccaatgata tgatcacaat cgtcgctgac tctgcagaac gtgctcgtga tatcgatatc
     6301 agtcgtgcag aacgtgccaa acttcgtgca gaacgtgcaa ttgaagaagc acaagacaaa
     6361 catttgattg accaagaacg tcgtgctaag attgctttgc aacgtgccat taaccgtatt
     6421 aatgtcggaa atagactata agaaaaaatg aacttgaaaa taccaagttc attttttatg
     6481 gtgttttaag gagcaaaacg gatgcagact gcttcgggaa catggaagtc gttggagagt
     6541 tctgctagac gaccattgtc acaattacgt ttaaagacag ttgcattgtc agagtcttga
     6601 tggacaacaa tgagaaattt ttggtcgggt gtcaaatcaa aatcacgtgg agtctgacca
     6661 tgcgttggaa cgatttctaa taactctaag ctaccgtccg caaggatggt atatactgcg
     6721 atagaatcat ggccacggtt agaagcgtag aggtatttac cgtctttaga gagatgaata
     6781 gcagcggttc cattaaagcc ttcgtaagct tctggtaaag ttgaaataac ctgcatacgt
     6841 acaaattcgc caacgccatc gtagattaaa acttcgatag tactattgag ttcacaaatg
     6901 agataagcga ttttatagtg gttatggaaa atgatatggc gtgagcctgc tcctggcttg
     6961 ctgtgatagg tatagagctt agataatttt ccttcttgat cgagatcata ggtgatgact
     7021 tggtcagttc ccaagtcgca ggtcactaga tagtggtcag gtgttaaatc tgtatagtga
     7081 acatgggggg aagcttgatt ttcatgtgga ccttggccac tgtgttgatc catatcacta
     7141 agtagaagac taccatcttc ctggcgttta taaacaagga cttgtccctt gtgatagttg
     7201 gctgcgtaaa ccaaatcacg cttttcatcg acagcaacat aacagtgggg agctccttct
     7261 tcaacaacat gatttaacac agtcccgtca gtttgatagg ctgcaattcc ccccttatcg
     7321 tcttggctac caacagtgta taaatgttgg tgctggtcaa aggcaaggta ggttggactt
     7381 ggctcagctg caaaaagttc tagatttgaa agctgaccag tttctgtatc aaagtctgcc
     7441 ttgtaaatcc cttgagaagt acgacgtgta taagttccaa aataaacagt ttctttcatt
     7501 actatacctc tatgtaaaga taagactatt atatcacaaa aacaagcaaa ttaaagacat
     7561 ccaat
//
LOCUS       DAVHIT010000093         7436 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVHIT010000093 DAVHIT010000000
VERSION     DAVHIT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.095"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            23..1693
                     /locus_tag="VVN16_001811"
     CDS             23..1693
                     /locus_tag="VVN16_001811"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU5573138.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2022..3197
                     /gene="mutY"
                     /locus_tag="VVN16_001812"
     CDS             2022..3197
                     /gene="mutY"
                     /locus_tag="VVN16_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU5573139.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            3252..3956
                     /locus_tag="VVN16_001813"
     CDS             3252..3956
                     /locus_tag="VVN16_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5573140.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            3949..5298
                     /gene="vicK"
                     /locus_tag="VVN16_001814"
     CDS             3949..5298
                     /gene="vicK"
                     /locus_tag="VVN16_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU5573141.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            5300..6109
                     /locus_tag="VVN16_001815"
     CDS             5300..6109
                     /locus_tag="VVN16_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5573142.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            6200..6442
                     /locus_tag="VVN16_001816"
     CDS             6200..6442
                     /locus_tag="VVN16_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU5573143.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            6444..6698
                     /locus_tag="VVN16_001817"
     CDS             6444..6698
                     /locus_tag="VVN16_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU5573144.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            6745..>7436
                     /locus_tag="VVN16_001818"
     CDS             6745..>7436
                     /locus_tag="VVN16_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU5573145.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENVFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIILVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLLGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLQDELS"
BASE COUNT         2224 a         1303 c         1678 g         2231 t
ORIGIN      
        1 taagaggtta gaggaatttt gaatgaaaac agatattgaa atcgcacaga gtattgagtt
       61 gaagccaatt gttgatgttg tagagaaact tggtatttct tacgacgatt tggagttgta
      121 cggaaagtac aaggctaaac tcagctttga taaaattcgt gcagttgaga gcaatccagt
      181 cggtaaattg atcttggtta ctgccatcaa cccaacacct gcaggtgaag gaaagtcgac
      241 gcttaccatt ggtcttgcgg atgccttgaa caagattggc aagaaaacca tgattgctat
      301 ccgcgaaccg tctcttggtc cagtcatggg gatcaagggt ggtgctgctg gtggtgggta
      361 tgcacaagtt ctgccaatgg aagacatcaa cctccacttt actggagata tgcatgctat
      421 tacaactgcc aacaatgccc tttctgcctt gattgacaac cacttgcacc aagggaatga
      481 gctgggaatt gatcaacgtc gtatcctctg gaaacgcgtt gtggacttga acgaccgtgc
      541 gctccgccat gtgactgttg gtcttggtgg tcctctaaac ggtattccac gtgaggatgg
      601 ttttgatatt acagttgctt cagaaatcat ggcaattctt tgcttggcaa cggacatcga
      661 ggacttgaaa cgtcgtttgg cgaatatcgt tattggttat cgctatgacc gtacgcctgt
      721 ttctgtaggt gatttgcagg ttgagggtgc cttggctttg attttgaagg atgctattaa
      781 gccaaacttg gttcagacaa tttacggtac acctgccttt gtacacggtg gtccatttgc
      841 caatatcgct catggctgta actctgtttt ggcgacgaca acagcccttc acttggctga
      901 ttacactgtt actgaagctg gttttggtgc ggaccttggt gctgagaaat tccttgatat
      961 caagacacca aacttgccaa catctccaga tgcagttgtt attgtcgcaa cccttcgtgc
     1021 ccttaagatg aatggtggtg tggctaaaga cgctctgact gaagaaaatg tagaggcagt
     1081 tcgtgcaggt tttgctaact tgaaacgcca cgttgaaaat atccgtaagt tcggtattcc
     1141 agcagttgta gctattaacg aatttgtatc tgatacagaa gctgaaattg cagccttaaa
     1201 agaactctgt gcctcaatcg atgtaccagt tgaattggct agtgtctggg ctgatggagc
     1261 agaaggtgga gtagcacttg ccgaaacagt tgttaagaca attgctgaaa atccagctaa
     1321 ctataaacgt ttgtacgata atgacctttc tgtccaagaa aagattgaaa aaattgtcac
     1381 tgaaatctat cgtggtagca aagtgaactt tgagaagaaa gctcaaacac aaattgctca
     1441 aatcgttcaa aacggttggg acaaattgcc aatttgtatg gctaaaactc aatacagttt
     1501 ctcagacaat ccaaatgcgc ttggagcacc agaaaacttt gaaattacca ttcgtgaatt
     1561 ggtaccaaaa ttaggtgcag gcttcatcgt tgccttaact ggtgatgtta tgaccatgcc
     1621 aggtcttcca aaacgtccag cagctctcaa catggatgtt gaaagcgatg gaactgtact
     1681 aggcttgttc tagtggattg aaattgactt taagtgagtt tggaaataat atccaagctc
     1741 attttttctc agactgcaag cagttcatca ataagattta tactcttcga aaatcttttc
     1801 aaactacgtc agcttcacct tgccgtaggt aatatgatac tgactctgtc agttctatcc
     1861 acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt
     1921 ttcattgagt ataagtagat agaaaagtta tatactatct attgaaaata ttataaatca
     1981 gatacaaaga caggtgaaac cttctgtgtt atactagaga tatgttagat ttgaaagaat
     2041 acggtatcgt catgtggccg gaggagaaag tcatttcttt ccgtgagaaa cttctcgctt
     2101 ggtatgatga aaacaaaaga gatttgcctt ggaggagaag taaaaatcct tatcacatct
     2161 gggtatctga aatcatgctt cagcagacca gggtggatac agttatccct tactacgaaa
     2221 gatttttgga ctggtttcca actgtcgaaa gtctggcaac tgcgcctgag gagagtttac
     2281 tgaaagcttg ggagggcttg ggttattatt ctcgagttcg caatatgcag gctgcagccc
     2341 agcaaattat gactgacttt ggtggccaat ttccaaatac ctatgaagga atttccagct
     2401 tgaaagggat tggaccttac acagcaggag ccatttccag tattgctttt aacttgcctg
     2461 agccagctgt agatggtaat gtcatgcggg tcttggcgcg tctgtttgaa gtcaaccacg
     2521 atattgggat tccaagtaat cgaaaaattt ttcaggcaat gatggagatc ttgattaatc
     2581 cagatcgtcc gggtgatttt aatcaagcct tgatggactt agggtctgat attgagtctc
     2641 ctgtaaatcc cagacctgaa gaaagcccag tcaaggactt tagtgcggca tatcagaatg
     2701 gaaccatgga ccgttatcca attaaatctc ctaagaaaaa gccggttcct atttatctta
     2761 aagccttagt tgtgaaaaat tctcaaggac aatttttact tgaaaaaaat gaaagcgaaa
     2821 agctcttggc aggtttttgg catttcccct ttatagaagt tgataacttt tcgcaagaag
     2881 agcagtttga cctctttcat caggttgcgg aagaaagtgt gaactctggc cccagtccag
     2941 aagagagttt ccagcaggac tatgacctag atgttgattg gcttgatgtt tgttttgata
     3001 cagtccagca tgtttttagt catcgaaaat ggcatgtaca gattgtagca ggtcaggtga
     3061 gtgacttcca tgatttttca gatagggaag ttcgttggct ttcaccagaa gaattcaaga
     3121 attacccact tgccaaaccc caacaaaaaa tctggcaggc ttatgcacaa gccaacttag
     3181 acagtagcca agactagcta ttgtgtcttc tgactatttt ttggtataat agtagagaaa
     3241 aaggtgaaca tatgaaaaaa atactaattg tagatgatga gaaaccaatc tcggatatta
     3301 tcaagtttaa tatgaccaag gaaggttacg aagttgtaac tgcttttaat ggtcgtgaag
     3361 cgctagagca atttgaagca gagcaaccag atattattat tctggatttg atgcttccag
     3421 aaattgatgg tttagaagtt gctaagacca ttcgtaagac aagcagtgtg cccattctta
     3481 tgctttcagc caaagatagt gaatttgata aggttatcgg tttggaactt ggggcagatg
     3541 actatgtaac gaaacccttc tccaatcgtg agttgcaggc gcgtgttaaa gctcttctgc
     3601 gtcgttctca acctatgcca gtagatggtc aggaagcaga tagtaaacct caacctatcc
     3661 aaattgggga tttagaaatt gttccagacg cctacgtggc taaaaaatat ggcgaagaac
     3721 tagacttaac ccatcgtgaa tttgagcttt tgtatcattt agcatcgcat acaggtcaag
     3781 tcatcacgcg cgaacacttg cttgagactg tctggggtta tgactatttt ggtgatgtcc
     3841 gtacagttga tgtgactgta cgacgtctgc gtgagaagat tgaagatacg cccagccgac
     3901 cagagtatat cttgacgcgc cgtggtgtag ggtattacat gagaaataat gcttgattta
     3961 ctgaaacaaa ccatttttac cagagatttt atctttatcc tgattttgtt aggtttcatc
     4021 cttgttgtga ccctcttatt actggaaaat agacgtgata atattcagtt gaagcaaatc
     4081 aatcaaaagg ttaaagattt gattgcagga gattattcca aggttcttga tatgcaaggt
     4141 gggtctgaaa tcaccaatat taccaataat ttgaatgact tgtcggaggt tattcgtctc
     4201 actcaggaaa atctagaaca agagagtaag aggctaaata gtattctgtt ttatatgaca
     4261 gatggggttc ttgcgaccaa ccgtcggggt cagattatca tgattaacga tacagccaag
     4321 aagcaactgg ggttggttaa ggaagatgtt ctgaatagaa gcattttgga attgctcaag
     4381 atagaagaaa actatgaatt gcgtgatttg attacccaaa gtccagaatt gttgctagat
     4441 tcccaagata tcaatggcga atatttgaac cttcgagttc gctttgcctt gatacgtcga
     4501 gagtctggct ttatttcagg tttggtggct gttttgcatg atacgacgga gcaggagaag
     4561 gaagaacgcg aacgaagact ctttgtttcc aatgttagcc atgagttacg gactcctctg
     4621 actagcgtaa aatcctatct tgaagccttg gatgaggggg ctttgtgtga aactgtagca
     4681 ccagacttta tcaaggtttc tcttgatgag accaaccgta tgatgcgcat ggtgacggat
     4741 ctcctccatc tttcacgtat tgataatgct accagtcacc tagatgtgga actgattaac
     4801 ttcactgctt ttattacctt tatcctcaat cgttttgaca agatgaaagg acaggaaaag
     4861 gagaaaaaat atgagttggt gagagattat cccatcaatt ctatctggat ggaaattgat
     4921 acagataaga tgacgcaggt tgtcgacaat attttaaata atgctattaa gtattcgcca
     4981 gatgggggta aaatcactgt cagaatgaag acaactgaag accagatgat tttatccatt
     5041 tctgaccacg gtttggggat tcctaagcag gatttaccac gtatctttga ccgtttctat
     5101 cgtgtggatc gtgctagaag tcgtgcacaa ggtggtacag gtctaggact gtctatcgct
     5161 aaagaaatta tcaaacaaca taagggcttt atttgggcca agagtgaata cggcaagggt
     5221 tcaaccttta ccattgtgct cccttatgat aaggatgcag tgaaagaaga agtatgggag
     5281 gatgaagtag aagactagaa tgagtgaaat aggctttaaa tacagtattt tagcgtcggg
     5341 ttccagtgga aattcttttt atctggaaac ctcaaaaaag aagcttttag tagatgcagg
     5401 cttgtctggc aagaaaatta ccagtctgct agctgaaatt aaccgtaagc cagaagacct
     5461 ggatgccatc ttgattaccc atgagcattc agatcatatc catggagtag gcgttttggc
     5521 tcgcaagtat ggtatggatc tttatgccaa tgaaaagacc tggcaagcta tggaaaatag
     5581 taaatatctt ggcaaggtgg attcttcgca aaagcatatt tttgaaatgg gtaaaaccaa
     5641 aacctttgga gatatcgaca tcgagagttt tggtgtaagc catgatgcag tcgcaccgca
     5701 gttctatcgc tttatgaagg atgataagag ttttgtcctt ttgacagata caggttatgt
     5761 cagtgaccgt atggcgggca ttgtcgaaaa tgcggatggc tatcttatcg aggccaacca
     5821 tgatgtagag attttgcgat caggttctta cgcttggcga ctcaaacaac gaatcctatc
     5881 tgaccttggt cacctttcta acgaggacgg tgctgaagct atgattcgga cgctaggaaa
     5941 tcgtactaag aagatttacc ttgggcattt atctaaggaa aacaatatca aggaactggc
     6001 tcatatgacc atggtcaatc agctggctca agctgatctg ggagtcggag tagactttaa
     6061 ggtttatgat acctcaccag ataccgcaac accattgaca gagatataga aagaacgctg
     6121 agaaggtgtt ctttttatat tgactgaaca cctaaaaagt aatacaatgg tgttaccatt
     6181 aaaaaaggga gcacaaaaga tgactactat tacattaaaa gtttctgaag ctgataaaac
     6241 atttatgaaa gcaatggcta agtttgaagg agtttccctt tcggaactta ttcgcaccaa
     6301 aactcttgaa gccctagaag atgaatacga tgctcgtgtg gcagatttag cctatcaaga
     6361 gtatttagaa gacttggaaa aaggagttga acccattact tgggaagaaa tgatgcatga
     6421 tttaggcttg aaggatgaat aatttgtata aattagttcc aacaagacgt ttcatcaagc
     6481 aattgaaaaa attggaccgt tatacgcaga agctaattac aaactattta caaatcaatg
     6541 ttttggaaga cccaagacga cacggaaagg ctttggttgg taatcgcgtt ggtcaatggc
     6601 gctatagaat tggtaattat cgagttatcg tacaaattgt agatgatgaa ttagttgttg
     6661 ctactctaga agttggtcat cggagagata tttattgaat taaagctgag taataacaat
     6721 atagaaagta aggagagtaa aaatttgaag aaaaaatggt tttttgcaga ttattatgat
     6781 acaacaatta ttttacttgc tttaatctct gttattctgg tattacttgg atttgctgag
     6841 atgattgatt tggacaatcc gccttatagt attattgatt tagtaatctg gggtgttttt
     6901 gtaattgatt atagttggcg cttttttatt actaaaagaa aatggcgctt tattcttgaa
     6961 aatgtttttg atttattggc tattctccct ttaaatgcta ttttcacagt ttttcgatta
     7021 gggcgtatct ttcgtttagt taaactaaca aaattactga aactgactcg tttacttaga
     7081 ataattggac ttacaggtaa attagaaaga aaaattagta gattcttacg gacaaatggc
     7141 ctgatttata tcttatatgt taatatcttt attattctag taggaagttc aattttatct
     7201 gtagttgaag aaaaatcctt ctcagatagt ctttggtggg ctcttgtgac agtaactact
     7261 gttggttatg gtgatattgt tccagtttcc cttttgggga aatggctagc agttttactg
     7321 atgcttgtcg gtattggaac aatagggatg ttaacaagtg ctttgacgaa cttttttgta
     7381 aaggataatc cagatgaaca gataaaactt gataaactcc aagatgaatt atctag
//
LOCUS       DAVHIT010000094         7398 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVHIT010000094 DAVHIT010000000
VERSION     DAVHIT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.096"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(76..1164)
                     /locus_tag="VVN16_001819"
     CDS             complement(76..1164)
                     /locus_tag="VVN16_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU5573146.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1182..1850)
                     /locus_tag="VVN16_001820"
     CDS             complement(1182..1850)
                     /locus_tag="VVN16_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU5573147.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(1869..4307)
                     /locus_tag="VVN16_001821"
     CDS             complement(1869..4307)
                     /locus_tag="VVN16_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU5573148.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(4458..5756)
                     /locus_tag="VVN16_001822"
     CDS             complement(4458..5756)
                     /locus_tag="VVN16_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5573149.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLACQNEIKQS"
     gene            complement(5773..6081)
                     /locus_tag="VVN16_001823"
     CDS             complement(5773..6081)
                     /locus_tag="VVN16_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5573150.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(6127..6447)
                     /locus_tag="VVN16_001824"
     CDS             complement(6127..6447)
                     /locus_tag="VVN16_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5573151.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(6642..>7398)
                     /locus_tag="VVN16_001825"
     CDS             complement(6642..>7398)
                     /locus_tag="VVN16_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEU5573152.1"
                     /translation="SLEIIPNEFDDNPTILSERISQVAAGVLRNLIDDNMKIGFSWGK
                     SLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYEMSRKFHGECTFMNATIV
                     QENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGKHQWLDMLTEDDFKELTK
                     VKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQSLAVAYGDTKVSSILSVL
                     RANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT         2422 a         1530 c         1062 g         2384 t
ORIGIN      
        1 tatttcaatc ttctatagaa ttcaatacag ggatcaataa aagactacca tttggaaaac
       61 agtagtcctt attgtttatt ttgaatttac ataggcatct acagcgataa tagcttgagc
      121 aacatctgaa ggcgaaatct taaacggcat ctgatgaatt gtctcaccct ccatagttgc
      181 ttgtttacca acttttatta aatcatcata tccaacttga tccaaatgca tttcttttag
      241 agttgttggc ataccaattt ttttgtaaaa ctcaatatac ttatcaagtt cttctttagg
      301 tctattttcc aataatagtt gtactaaagt tccataagct actttttcac catgtgttaa
      361 atgatgaatg tcacctgtca atgcagtaaa accattatga attgcatgcg ccgcagctaa
      421 tcctccactt tcaaaaccta gaccactcaa taaagtatta gcttcaacaa tattttctaa
      481 tgctggtgtc accactttag cttcacaagc tgccatagcc tgtaaaccat ctgcaaacag
      541 cgtttcttca catttcttcg caattgcaac tccagccaat gtttgctgtt gtcccaacat
      601 agtttttcca tttgcctgca taaccgcacg cgcctcaacc caagttgcta aaccatctgc
      661 aataccagac gctaataaac gcttaggggc ttgtgaaata acttttgtat caaccaaaac
      721 taaatctgga tttttagaat aaaatagata atgatcaaat gcaccttcat ctgtataaat
      781 aacagataaa gcagatacag gtgcgtcggt cgatgcaatt gttggagcaa taataacagg
      841 cttttcaatc aaatctgcaa tagcttttgc gctatcaatc gtctttcccc caccaagacc
      901 gataatacta tcacaatttt ctttctcagc caaggcaaca actcgattga tttcattgtc
      961 agaagcttca ccattaaata gcgccagaac aatatggaaa ccatacctat gtagataatc
     1021 ttcaaatcgt tttccaacaa tatcataaac caactgatcg cataatagaa tcgggtaatt
     1081 tcccaaatcc aaaattgatt tggcattttc aaacaaggca ttttccccct gaatatatct
     1141 agaaggacta gcaaaaattc tcatatgtat ttcctctcta tctaaatgga acgactattt
     1201 tgagtaacaa accaatcgtc agaaaaatca tcaaccgcct tttggataga tggcatggcg
     1261 aaagctgatt caaaaacatc tgctcctgct gtaacagcat gcgcacctgc agctaaagca
     1321 ttatttactt gtgctacatt tttaaaggat gcagctaaaa tcttactagg agagttctgt
     1381 ctatcaatag caagagctaa ttgacgaatg acagaatttg aatcaatgtt cagattttcc
     1441 attctattat aatatggagc taggtaatcc gctcctgctt cgatagctaa taatccctga
     1501 ataactgtat aaatagctgt tgcagtgata tggtagccct ctttttttag cacctttatt
     1561 gcacgtaatc cagctggagt aacaggtact ttgataaata tatcatctcc tgcttgtctt
     1621 cgaatttcat gagcatcctt taagatgcct tcaaaatctt gagaaatcac ctgaacatga
     1681 atagagggtg tagagccaat caattctctt acatctttga ttcgttcaaa aaaattaata
     1741 gaaccctctc tttttgcaat agtgggattt gaagttaccc cagctagcgg caaaatttca
     1801 gaccactttt taatctcatc taaatttaat gtgtcaagca taaattccat aaaaagaacc
     1861 tctttatttt acaaagtatg ctcagtacgt cctataatgt catcttgggt tgccttagaa
     1921 agaacattga agaatgcaga gtatcctgca acacgaacaa ttaagtctct gtgtttttca
     1981 ggatgtttct gagcgtcaat cagcgtctct ctggaaacaa cattgtattg aatatggtac
     2041 ccatgtaaac gattaaagaa tgttcgtaac aaagcaatta gttttaattt atcttcttct
     2101 ttggctaacg tttgaggatt tactttctga tttaagagaa ccccacctac gatttcatct
     2161 gttggtaatt ttgaaacaga ttttaaaaca gatgtagggc cgtgttgatc catattatgt
     2221 gatggtgaac aaccctctgc taacggtgta cccgcgttgc gtccatctgg agttgctaat
     2281 gttccacgtc cctgccctac gttggctgag atagaagatg ttcctgaata acgaattcct
     2341 ccaattggac ctcttccata acgtgtatta ggatatttag caatttcatc aacataaatg
     2401 tcataagcag cagtaaccaa tttgtcagca taatcatcat cattaccata cttaggtgca
     2461 tcatgaatca acatttcttg aatgacctta ccttcttctc cggcataatc tgtttccagt
     2521 gcatgccaaa gctgacttgg gcttatacgt tcttcctcaa acaccaattt tttaattgca
     2581 gctaatgaat ccgacaaatt tgcaattcca acttgcaatc ctgatatata atcatatact
     2641 gctccacctt ctttaaggtg ttttccacga ccaatacaat catcagtcaa tgctgaacat
     2701 agaatatcag gaacttctcg ttccaatgat aaatcaatag aattttcaac aataacactc
     2761 attcgtgtca aatatcttag tgttttatcc caagcatttt ctaattcaga aaagttcttc
     2821 atatccttaa aatgaccaaa gcttggtgca aaccgtttac ccgaagccgg atcaattcca
     2881 tcattcatcg tgataagtag aaccttaggg aagttcatat aactcatacc tgtgcaacga
     2941 tagccccatt tccccggaac tgccgtttca acacatccaa tggcactgta atcataagca
     3001 tcatcttcca atactccttt tgcaataaaa gaaggaataa taatctcatc attattaaat
     3061 gcaggcatac caaaaccaag tttcatcact tcaatacact cattcatgaa acgagcatct
     3121 aaacctgcat ggtaacgtac agttagatta ggttgcggta gatgggtttg tgcaactgat
     3181 tttaatacca aataagataa tgggttaaca gcatccttct tatctcgagt ctgtccacca
     3241 attgtaacat tttgatataa aggacttcct gctgaagaaa atgtatgtga ttgactgcga
     3301 accttattaa ttgtaattgt cttaatccaa agatttgtca gacgttcaac aatgctatct
     3361 tctgtttctt taccactttc taaatcagcc ttcatatatg gatacatata ttgatcaaaa
     3421 cggccatatg aaagagagtg gccattagat tcaatttgta aaatacattg aataaaccaa
     3481 actgattgaa tagcttctgc aaaagtagtt gccggttcat atgggactct agagcaaata
     3541 tctgcaatct caagtaattc tttcttacgt ttaggatttg cattttcggc taaactttta
     3601 gcaagagcaa caaagcgctt tgcatatact ttaatagcat cgattacgat aaatatagag
     3661 tcgtaaaaat gatatttatc aatacttgct ggatctgtta aatctagagc taccttcgct
     3721 ttacgagccc gctcttcaaa acctcttaaa ccaaattgca aaagtttctg atagttaact
     3781 gctaagtgag catctccaga attcatctta ccttccatac cgaagaatcc tgtttccata
     3841 taaacagaca cttcttcagg taataaggca ccagctctag cacgtaaatt attattttcc
     3901 caaaacggag caatacttct aagttgttct tttgtttctt ctgtaatata gaaaacatct
     3961 ccatcacgct tttcaaaaag atccaactca ttgagaacaa attctagcgt atattccgga
     4021 aaaataggag catctttatt ggaagaagct tgatttcccg caatcataga ttcttcttca
     4081 atatagatag acatattttc caaaatttct ttcagcatat atgcacgttt taggacatta
     4141 ggttgttcct tgtatcgttc ataggcgcgt gttgctagaa ctgctctctc agcatcaata
     4201 taaggttttt tatttaaaac atcttctcga tatttgttca tcctttcagt aaggctgcca
     4261 aaatattctg tcttgattgt tgttcttgct acttctgtat ttaccatcgt aagcaccttt
     4321 ctttcgaaaa tgtttttatt tctttcgaaa atataatacc gatttttata gaaattttca
     4381 agcttttaac tagaaaaatt ttaactctct aatttttagc acaaatgtgt gagtttaaca
     4441 gtaacacaca aatacctcta agattgtttg atttcatttt ggcaagctaa acgatcttgt
     4501 atcttaaaga atggaaagta aaccattgta gacatcgcca atactaataa ctgagtcaca
     4561 acaccttgcc atccacccac caaaaatcct gatagaatag ctggtgtact ccaaggcaat
     4621 gttacccctg agaatggttg catgaaacct gttgcaatag ctccatatac tatcacagct
     4681 gcaagtacag gaacaagaat gaaaggtaca aacataactg gattcatgac aatcggaaat
     4741 ccaaatacaa ctggctcatt tacgttaaat attgctggaa aagctgcaac ttttcctaag
     4801 gcttggtatt gttttgattt tgctgcaaaa agcatggcaa ctacaagacc aaacgtaatc
     4861 cctgaacctg atagaattaa aaatgaatct aaaaattgtt gagtaacaat atgtgcacca
     4921 ttttctaatg atagattacc agaggctaac atagctttat tagcatcaag attagataaa
     4981 agcagagctg tcactactcc atttactacc gattgcccat gaacaccaaa ccaccacaaa
     5041 aatgatataa agaatgcaat tccaatagca ccatacaaag atccagttaa accttgcaac
     5101 ggaacttgaa tagcagaata aatcatttct atgaatgttc cgccattagt caatgacttc
     5161 gctaaaatat atacaatcat agaagataag aaaattacaa atgctggaat cattgcttca
     5221 aactgtttgg caatagcttg tggaacttgt tctggcatct taataacaat ttttctcttt
     5281 ataaagaagg tataaatact tcctactacc aaacctataa tgatagcacc gataattcct
     5341 tggcctccaa accaaacttt actaatagcg tccccaatcg cctcaccttg tttagggata
     5401 taagatgatc ttagcaaaat aaagaatgca gatacagata gaactccagc tggtaaagcc
     5461 tctactccgc tattcttagc ataagaatag gcaattgaaa aacaagaaat tagacccata
     5521 atagcaaaag ttcctgaata tacttgcata aacggctctg tccaattagc tccaaaaaca
     5581 ctagcaatgc tcttatttaa tccttcgaac ggcaattgtc ccataatcaa gaacaaactg
     5641 ccaactactg tcaatggcaa aattgctaac atcccatctt ttagagctat aatgccacgc
     5701 atattcacaa acttcatcat cggtgcaatg attttctgaa catccatctt tgacataata
     5761 aatctccttt tcttacccac taatcaaaga tagggccaaa tctaatactt ttttcccatc
     5821 taacatacca tagtccatca tcggaataac agctatcgga acatcacact tatcacaaat
     5881 ttcttttgat ttatctaatg tataagcaac ttgtggaccc aatagtgcaa catctatatt
     5941 tggcgcataa tccgctaatt tagactgaga aaacgcctct atttctgcct caactccact
     6001 agattgcgct gcaattttca tattatttac aagcatacca gtagaaaaac ctgctgcaca
     6061 aaacaaacca atcttcacca ttatgttttc ctcctctatg ttaataacaa tgataatact
     6121 ctagtattat tttttatgaa gttcttttct caaactaata atttctttga ttaaattaat
     6181 ctccgtcata ctagtcatga gatgatcttg tgaatgaaca aagagagctg taatttctgt
     6241 ctttgtacca gacgcttcct gtgccaagaa ttttgtttgt agattatgag cttctaataa
     6301 ggctgcatca gctaactgga tttcttcttc acatcgttca catgtaccgt tctttatgta
     6361 atctaatgct ttataaatat gttgtttcgc atcgccagca tataaaatta aacccatgat
     6421 aatttgatct ggaacaataa tttccataaa ttcctcctca tttcactatg taaaaagctt
     6481 ttttcatttg aaattttaaa attttctttt gtaactatag aatacaacta cataataaag
     6541 ttgtcaatcc aaaaacaaca gataaactat ttttgtttat ataagcacaa atgtgcaatc
     6601 aactaaaaag aaaaacgata aactgaatca gtctatcatt ttcactcacc tagaatctct
     6661 ctaaaagtca aatccccatc ttcttccaaa acttttaaaa ttgtattttt gtctgtaatc
     6721 aaatgattta ctaaattagc acgcaagaca gaaagaatcg aagatacttt cgtatcaccg
     6781 taagcaacag ctaaactttg aggaatattt tttaaatctt ccaaagagat tgctatcgtt
     6841 ctttcctgta agttttcata gacttcttta ccttttgaat caaaaaatcg acaacaaatt
     6901 tctcctacag ttttaacttt ggtcaattct ttaaagtcat cctctgtaag catgtctaac
     6961 cattgatgct ttcctttatt gctaaaatca ccaattccga ctacagctat atctaaatct
     7021 ttccaactat ttttcaaatt ttcaaaatat cttgattgca aaataccatc tgctaacaat
     7081 ttattttctt gcacaatcgt tgcattcata aatgtacact ctccatgaaa ttttctagac
     7141 atttcataaa tcagtgtatt cacatggtat ttagcgtgta tgtgactagg accacctgct
     7201 agaggataga agtgaacatt tcggacactt ttactgtgaa ttaaatctac taaattactt
     7261 aaacttttcc cccaagaaaa gccaattttc atattatcat caattagatt cctaaggaca
     7321 cctgctgcaa cttgagaaat tctttcagat aaaattgttg gattatcatc aaattcattt
     7381 ggaataattt ctaaactt
//
LOCUS       DAVHIT010000095         7168 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVHIT010000095 DAVHIT010000000
VERSION     DAVHIT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.097"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            31..342
                     /locus_tag="VVN16_001826"
     CDS             31..342
                     /locus_tag="VVN16_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573153.1"
                     /translation="MLSGLIFFLAQGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGV
                     LTIDFLFFVLLAFISSQRFSSLMSIITACHSTFYYMTADYLRENYPDFYDKHISLWEC
                     L"
     gene            361..544
                     /locus_tag="VVN16_001827"
                     /pseudo
     CDS             361..544
                     /locus_tag="VVN16_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(757..1200)
                     /locus_tag="VVN16_001828"
     CDS             complement(757..1200)
                     /locus_tag="VVN16_001828"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU5573154.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(1230..3452)
                     /locus_tag="VVN16_001829"
     CDS             complement(1230..3452)
                     /locus_tag="VVN16_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5573155.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IVANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            4411..5040
                     /locus_tag="VVN16_001830"
     CDS             4411..5040
                     /locus_tag="VVN16_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5573156.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKYLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFHFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGSATTIAHEKAFNPFF"
     gene            complement(5163..7055)
                     /gene="pepO"
                     /locus_tag="VVN16_001831"
     CDS             complement(5163..7055)
                     /gene="pepO"
                     /locus_tag="VVN16_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU5573157.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEESWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         1931 a         1692 c         1371 g         2174 t
ORIGIN      
        1 attagactcg agacatcttt tgagtggctc ttgctctctg gtttaatttt cttccttgct
       61 caaggactcc tgctatttct cttgatcgtc cgactcaaac atcaattcgc tgagatttat
      121 cctcaaatca ataaaaagat tcgcttctac tatttagggg ttctcaccat tgattttcta
      181 ttttttgttc tcttagcctt cattagttct cagcgttttt catctcttat gtcaattatc
      241 actgcttgcc attctacttt ttattatatg acagctgact acctaagaga aaactatcca
      301 gacttttacg acaaacacat ctctttatgg gagtgtctct aaggaaaagg aggttttagc
      361 atgaaaaaaa tcatcttcat caaaaccatt caactccttg tcattgatgg aatcatgctg
      421 gcatttttga catttaaaag gggggcttac ttgggactgg attttgattt atagtggttg
      481 gctcattttc tttcatcctg tgctattgac ctatctttca aaccaacttt gtgaccactt
      541 tagtcaactc tattcccaga ttagaccgag attctggcgt tttgctttac aaattctcct
      601 atgggatagc ctgatgattc tctccttggt gtctttaagt gatattccac ttttccttca
      661 gggaactctc ctcatcctag gacatctcat cccttcctat cgcatctgcc aaagcctgaa
      721 aagagacttc ccccaagcat atcaagaacc gatttcttat ctctttttag tatctaggat
      781 aatggtaaca ggtccgttat taaccagctc aacctgcata tctgctccaa agatacctgt
      841 ctgaacgggc acttcttgcg ctaatttttg attgaaagca tcatagaagt ctgatgccat
      901 atcaggttta gctgcccctg taaaggctgg acgattgcct ttcttagtat ccgcaaagag
      961 ggtaaactga gaaatagaga ggatttctcc ttcaatatct ttgacagaca ggttcatctt
     1021 gccttctgcg tctgaaaaaa tccgcatatt gaccagtttt ctcacagcat agtccaaatc
     1081 ttcctcttgg tcctctggtc caacaccaac cagcaataaa agtccctgat tgatttttcc
     1141 ctgaatctgg ccttctatac tcacttgggc ttttttaacc cgttggataa tgattttcat
     1201 aatagccttt ctagtaagag ctaggacaac tagccgttgg tccgtttgac agagtaaact
     1261 tctggcacac tcttaatttt atcgacaacc gtggtcagtg tagagaggtt ggcaataccg
     1321 aaggacacat ggatattagc aaacttcata tccttggttg gttgggcatt gaccgttgaa
     1381 atattcttgg ttgtatttga aagaacttgc agtacatcgt tcaacagtcc tgtacggttg
     1441 agaccgtaga tatcgatatg ggccatatac tccttatttg agctagagta ctggtcttcc
     1501 cattccacat caaggagacg ttgctcgtag ttttcttggg cacgcaggtt catacagtcc
     1561 acacggtgaa tagccacacc acgacccttg gtaatgtagc caacaatatc gtcaccaggt
     1621 acggggttac aacacttagc aatccgcact aggagaccag aagcaccttc aataaccact
     1681 cccccctcat gcttgacctt gagagtttct ttattttcaa ccttgacctc gccacctttg
     1741 acaagctcct ctgcctcagc cttggccttg gcacgctctt cctcacggcg ctccttttca
     1801 gtcagacggt taaagacggt aatcgcaccg atttccccaa aaccaatggc cgcaaagagg
     1861 gagtcttctg tcttgtaact ggtcttttgc agaacttgat ccatgtggcg cttgtccata
     1921 aatttatttg ccacatagcc attttcttgg aactgagcca tcagcatctc acgacccttg
     1981 ttgacagaca attccttatc ttggttttta aagaactggc gaatcttatt gcgcgccttg
     2041 ctagtcttga ccatattgag ccagtcacgg ctaggtccaa aggagttcgg gttggcgaca
     2101 atttcaacct gatcccctgt ctttaacttg gttgtcagtg gaaccatgcg gccattgacc
     2161 ttggcaccag ttgctttttc accgaccttg gtatggattt cgtaggcaaa atcaatcggt
     2221 cctgaatctt tgggaaggga acggacagct ccatctgggg taaaaacgta aatctcctca
     2281 gccaaatagt tttccttaac agagtccaca aattccttag catcatcagc ctggtcttgg
     2341 agctccatca tctccttgat ccagttcatt ccaatagctg attccttgct gttaacttgc
     2401 ccctttatac ctttcttata agcccagtga gccgcaaccc cgtactcagc cacctcgtgc
     2461 atttccttgg ttcgaatctg gaattcaatc ggcccttttg gtccataaac agtcgtatgg
     2521 atagactgat aaccattggc cttgcggttg gcgatatagt ctttgaagcg acctggcatc
     2581 ggtttccaaa attcatgcac gtaaccaagc atggcataaa catcactttg ggtatctaaa
     2641 atacaacgaa tagcaatcag atcatagatt tcctcaaacc gttttctctt gtcctgcatt
     2701 ttgcggaaaa ttgagtaaat atgcttggga cgaccataaa tcttcccttt caagtgacgt
     2761 tctgtcgtat actcctctaa ttttgtgact acctcatcca ccaaggcctc acgctccctg
     2821 cgcttttcct tcatcatatg ggtaatcttg taaaactccg ttggattgag ataacggaaa
     2881 gacaagtctt ctaattccca tttgacactg gaaatcccca aacgatgggc aagcggggca
     2941 tagatttcca tggtttcttt ggaaatacgc tcctgcttgt cttttcgaag atgtttcagg
     3001 gtccgcatat tgtgcaagcg gtcagacagt ttgaccaaaa taacgcggat gtcctcagac
     3061 atggccatga gcatcttgcg atgattttcc gctaattgct cctcgatcga tttgtactcg
     3121 accttgccaa gcttggtaac tccgtcaaca atcacccgca catcaggacc aaactctctt
     3181 tccaaatcgt ccaaggtcgc atctgtatct tccaccacat catgcaagaa tccacaagct
     3241 actgttacag catccagctt tagcttagct aaaatacctg ccacttggat agggtgaatg
     3301 atataaggct cgcctgattt gcgatattga ccactgtggc attcaacagc atagaccaag
     3361 gccttatgga caaaatgaac atcctcttcc gttaaatatt ctttggttaa agcgacaact
     3421 tcttcgcctg ttaaattcac ttctttcggc atctctactc tccaattctt cctaccattt
     3481 tatcactttt ttaagaatat gaaaactaga ttggaacaga ataagaaaaa aataattcaa
     3541 aattgcttga taattctgaa ttatcggccc gtaatatact acgaagttag attttaaact
     3601 taggtgatag aaggagagat agaagaacgg aaaccatatt gtaacccaaa gactttctga
     3661 cttccccaat tccattgaag atacgaaaga taaacggtgg aactcgtatc acatacactg
     3721 gtaccttgac tggattttgg aattaatact aaatgaaaat caaagagcaa actaggaaac
     3781 tagccgcagg ttactcaaag caccgctttg aggttgcaga taaagttgac gcggtttgaa
     3841 gagatttttg aagagtataa aaatcctcaa gatactttct tctatccttt agtttataag
     3901 gagaatacct atgaaaaaaa ctgctatttc tatctttgct ctcctaatgt taggagtttg
     3961 ctgcctgttc ctattcagcc agcaaagcta taaaaaacag tcgttcaata ctatgctaac
     4021 gaccagaacc tgcccagtag gataacttat agtgaatata gcgacaaatg agaagccaac
     4081 tacggtagca ctctaaacat cacgtctatc aaacaagcta atgacggagt ttatgcaacc
     4141 tatgaagggc aattgacacc tttccaatat tgataaattg ataaccagcc tgtcttcatc
     4201 tagtcatgct ggtttttaag ttcattttaa atccttacct attctcccta actgtgctat
     4261 acttaattta tactcaatga aaatcaaaga gcaaactaga aagctagccg caggctgttc
     4321 aaagcactgc tttgaggttg cagataaagt tgacgcagtt tgaagagatt ttcgaagagt
     4381 attagtacat tctttgagat tggagctagt atgaaaatcc ataaaaccgt gaatcctgtt
     4441 gcctatgaaa atacctatta tctagaaggc gaaaagtacc tcatcgtcgt cgatcctggt
     4501 agtcattggg aagccattcg tcagacaatc gagaagatca acaaaccgat ctgtgctatt
     4561 ctcttgaccc acgcccatta tgaccatatc atgagtctgg acttggttcg cgagacgttt
     4621 ggcaatcctc ctgtctatat cgcagagagc gaagccagct ggctctacac tcctgtcgat
     4681 aatctctccg gtctccctcg ccacgatgat atggcagatg tggtcacaaa acctgcagaa
     4741 cacacctttg tctttcacga agaataccaa ctagaggaat ttcattttaa ggttctgccg
     4801 accccagggc actctatcgg tggtgtttcc ctagtctttc ctgatgctca cctagtcttg
     4861 acgggagatg ctctattccg cgaaactatc ggacggaccg accttccgac tggtagcatg
     4921 gagcaactcc ttcatagtat ccagacccaa ctcttcaccc taccaaacta cgatgtctat
     4981 ccaggacatg gttcagctac tactatcgct cacgaaaagg ccttcaatcc ctttttttag
     5041 caagatgatg acaatcgaaa tttaagtaaa ctatccagca aatctttcta ttataaaagg
     5101 catcctatca aggttttcac acgtgattgg atgccttttt tctgatgact agattttttg
     5161 tattaccaaa taatcacgcg ctcctctggt gaacgccaca ttccgtctcc ttctttgaca
     5221 tcataggttg taaagaaatc gtcgaagttt ggtacttgca cattgacacg gagtttggct
     5281 ggtgcgtgca catcgacgct agccaaaagt ttcataaatt ctggacgacc tttcatgcgc
     5341 cagatgcgac cgaagttgta gaagaactct tctgctgaga agtctgcttc tctcttagct
     5401 gcttcaagcg ctgctgcgat tcctcccaag tcagccacgt tttctgatac agtcaattta
     5461 ccgttaatgg ttgctccata agaatcctgt ccatcaaatt ggtcaatgac tttttgtgtt
     5521 ttctccttga aggcagcata gtcgctctct gtccaccaat ccttgaggct accattttcg
     5581 tcaaaggaag ccccgttagt atcaaaggcg tgggaaattt catgggcaat cactgcccca
     5641 ataccaccgt agttagcaga agatgactga tgcaagtcat agaaaggcgc ctgtaaaatg
     5701 gccgctggaa agacaatcag gttcttctga ggattgtagt aggcattgac catatgagca
     5761 ggcatgcccc attccttata gtctacaggc tggttccact tactccaact gtgcttgatt
     5821 tccacacgcg caaaggctag agcattctca aaaagactgg cagtttcatt cactacctta
     5881 tccttgtaac gtgcaggcaa ttcttctgga tagccaatat aaggtttgat cacattgagc
     5941 ttcacgatag cctgtttaca ggtttctgga gtgagccagt cattcttaag cagacgctcc
     6001 ttatagacat caatcatggt tgccactttt ttctccacat ccgccttggc ttctggagag
     6061 aacttctcac gggcgtacca aagccccagg gcttgcttga aaggttcttg tgctagatga
     6121 taagctgctt tgaccttatc ttttgcctct ggaactccag aaagggcacg gctgtaggca
     6181 ccagacaaaa cacggatatc ctctgttaaa tagctggttg aaagattgac aacactcaaa
     6241 atcaaggttg ctttaaggag agaccaggat tcctcactgt agaattgctc tgctgcttgc
     6301 cagaaacgtt cctcgtctac aataaccttg tctggtaatt gcccaataac tgctttgaag
     6361 aagtcatcca aaggtagggc aggcgcgaat ttcttgaaat cttcgtaaga atatggatga
     6421 tagagtttag catattctga actttcttca ttagagagca ccactgccgc aactcggcgg
     6481 tccaattcaa gtcttttttc tagcaagtct tcaatttctt catcagagaa atcataagcc
     6541 ttgaggagat ttgcgctgct ttctttccaa agagtcaaga gctcttcgcg ctgaggatgt
     6601 tcttctgcat agtaggtcgt atctggcaag attgtgcttg gagcgctagc ccatagaaca
     6661 ttgattctag catccataaa gtctggcgat acaccaaaag gaaggaagtt tggttttcct
     6721 gcaagctcaa actctgctag tttagctgta aaatccgcaa aagtttccaa ttcttggaat
     6781 tctttaagga gtggtaagac aggtgtgata ccgtcagctt ctctcttgtc aaaatcacga
     6841 actaggcggt ggtatttgac aaagttttcc aagatagcat cctcaggcac ttcttcacct
     6901 gctaaccact tgtctgttgt cgccaacatc aggtcttcaa tttcctggtc taaatcaaca
     6961 aaacctcctg tttgagactt atctgctggg atttcagctg tctgttgcca ttctccattg
     7021 atagcatcat aaaaatcatc ttgataacgt gtcatcttgt tctcgctttc atttgtattt
     7081 gcatttatct taacaaaaat cactagggaa tgcaattaat aaccatttaa ctattatttt
     7141 ttagctatta taaaaaatct ttccatga
//
LOCUS       DAVHIT010000096         6222 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVHIT010000096 DAVHIT010000000
VERSION     DAVHIT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.098"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(121..396)
                     /locus_tag="VVN16_001832"
     CDS             complement(121..396)
                     /locus_tag="VVN16_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5573158.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(503..1342)
                     /locus_tag="VVN16_001833"
     CDS             complement(503..1342)
                     /locus_tag="VVN16_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5573159.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILCHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1484..2332
                     /locus_tag="VVN16_001834"
     CDS             1484..2332
                     /locus_tag="VVN16_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU5573160.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2425..3342)
                     /locus_tag="VVN16_001835"
     CDS             complement(2425..3342)
                     /locus_tag="VVN16_001835"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU5573161.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3780..3985
                     /locus_tag="VVN16_001836"
                     /pseudo
     CDS             3780..3985
                     /locus_tag="VVN16_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4190..4483)
                     /gene="rpmA"
                     /locus_tag="VVN16_001837"
     CDS             complement(4190..4483)
                     /gene="rpmA"
                     /locus_tag="VVN16_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5573162.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4500..4844)
                     /locus_tag="VVN16_001838"
     CDS             complement(4500..4844)
                     /locus_tag="VVN16_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU5573163.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4860..5174)
                     /gene="rplU"
                     /locus_tag="VVN16_001839"
     CDS             complement(4860..5174)
                     /gene="rplU"
                     /locus_tag="VVN16_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5573164.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5210..5297)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5362..6060)
                     /locus_tag="VVN16_001840"
     CDS             complement(5362..6060)
                     /locus_tag="VVN16_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU5573165.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
BASE COUNT         1927 a         1280 c         1019 g         1996 t
ORIGIN      
        1 tatatataca atattttcgg aacattcaac tttttaactc tatttattac tagatttcat
       61 aattaaaaag cctactgacc aagctagaaa gcttgataca ataggctttt taaagactga
      121 ttatttaaca gcgtctttaa gagctttacc agctttgaat gctggtactt tagaagctgc
      181 aattgtcatt tctttaccag tttgtgggtt gcgaccttta cgttctgcgc gctcacgaac
      241 ttcaaagtta ccaaaaccga tcaactgaac tttttcacca gctgcaagat agtcagctac
      301 tgctgcaaat acagcttcaa ctgctgctgc tgagtctttc ttagtcaatt ctgtagcttc
      361 tgctacttta gcgatcaaat cttgtttgtt tgccatgtta atgattcctc caaataattt
      421 ctaattaaca aatataatca tatcctaaaa tcccttttag gtcaagttaa aaacgctatt
      481 tcttcccatt ttatttattt ttttaggagt ggtaacgtat caaaatagcc caagcgttct
      541 cacccgtgtg agtttgaata atggaacccg tttccaaaac agaaattggc ttttcaacat
      601 aagcttgtaa gctttctttc atctcttttg cccaatcatc actaccagaa tatgaaattc
      661 caatctctgc tacagcacgt tcagaaagcg atgttatcaa ctcatctaac cattttttaa
      721 atgttttagt tccacgacct ttaaccattg gctgcaattc atggtctttc atttgcatga
      781 caacacggat attgagaaga gagctcaaca agccagttac acggctaatt cgtccacctt
      841 tgacaagatt ttccaaagtt gaaacaccaa tataaagctc tgtatggttt ttaacctctt
      901 ctacatgaca taaaattgcc tccatatctt taccttcttg agctaacttc gcagcctcaa
      961 caacttggaa tttcagggct tggtcagtga aggaactatc aacaacagtc acatctgcag
     1021 tagataggct agcaccttgg cgtgctgctt ctaccgtacc cgaaagagca tgggacatat
     1081 gaatagcaag aatctggcca ccatctttgc ataggtcttc aaaaatctca gcaaagacac
     1141 ctacaggtgg ctgacttgtt ttcggaagat tcttactttc ttgcatcaac tgaagaaatt
     1201 taccttcttc tttcaaatcc gcatcagaat aaacaacatt atcaatcatt acagataatg
     1261 gaacaattgt aatatctaat tgctttacta gttcaggttc aatagtaaca gatgaatcgg
     1321 ttacaatctt aatttttgtc atagtatcaa tctttctatt ttaggattca gattggtttc
     1381 cttacttcta attatatcaa aaaaaagatt aaaaatccta atggagtcaa tcaaattttc
     1441 cgtaaaattt gatataatca acttataaga aaagaggtgt cctatgatta aaaaaattta
     1501 ccccattttt accattttac taggtgctgc tatttatgct tttggactga cttattttgt
     1561 agttccccat catctctttg aaggaggggc gacaggcatt accctcatca ccttttatct
     1621 ttttaaaatc cctgtttccc tcatgaacct gctgattaat attccccttt tcatcctagc
     1681 ttggaagatc tttggagcca aatccctcta ttctagttta ctaggaacct tagctttgtc
     1741 cgcctggtta gctttttttg agcatattcc ccttcatatt gatcttcaag gtgatttact
     1801 aatcacagcc cttatagcgg gaatcctatt gggaattggc cttggaatta tttttaatgc
     1861 tggaggtaca actggcggaa ctgatattct agctcgtatt ctcaacaaat acactcatat
     1921 atccatagga aaactgctct ttatcttaga tttttgtatt ctcatgttga ttctcctaat
     1981 cttcaaggat ttgagattgg tttcctacac gcttttgttt gattttattg tttctcgtgt
     2041 tattgatttg attggtgaag gaggatatgc cggcaaaggc tttatgatta tcacaaaacg
     2101 tcctgaccaa cttgctaagg cgattaatga tgacctcgga agaggtgtta cttttatttc
     2161 tggtcaaggc tactatagta aagaaaattt gaaaatcatc tactgtattg tcggaagaaa
     2221 tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat cctcaagcct ttataactat
     2281 tacagaagcc catgaaatcc taggagaagg cttcaccttt gaaaaagaat aaaaagaggt
     2341 aatgtcgtga cctcaaaagt tagactaaat catctatctt ttgggttaca gacaacctct
     2401 tttttatttt atttactcaa gctcttaaga ccaattccga gttacttctt catcagcctt
     2461 taactgatcc actaattggt caactgagtc aaatttggtc atatcacgaa tgcgatcaag
     2521 ccaataaacc atgacggttt ccccataaat atcttgatta aaatcaaaaa tattgacttc
     2581 aaaacgtgct tcttctccat caaaggtcac atttttcccg acactagcca tagcacgata
     2641 cttctgtctt tgaatctcaa catcaacgac ataaacgcca tctgctggca tataagtacg
     2701 gtctaaaagc actaaattcg ctgtcggata accaattgta cgaccacgag cattaccatg
     2761 aaccaccata cctcttgatg gaagcggtgc ccccaaaagt tttcctgctt ctttcacatt
     2821 tccatctaaa atagcttgac ggatacgagt tgaactaatc tttcctttct catcttctac
     2881 aggtggaaca atgataactt ctccatcaaa gtaatccttt aaatcttctg ctgttttttt
     2941 gtcagaacca aatgtataat caaaacctgc aacaataatt ttggcattca tagccttgat
     3001 ataagttgca aagaattctt gtgcagtgag actagcgaat tgactactaa aatcaaggag
     3061 atataattct tctacacctt cgcgctttaa ttttctttca cgttcagcag ggttcaaaat
     3121 atgcaaaaac aaatctggat gataaggctc taaagcgatc tttggagatt cattaaaggt
     3181 cataacgacg ataggtaata aatcctttct cgcagccttg ttggcaacac gaaataattc
     3241 ttgatgcccc ttatgtatgc catcaaaata gccgagaaca acgactgaat cagatggtgt
     3301 gccaatatct ttttggtttt ttataggaat agtaataatc ataaaataat tatatcatag
     3361 cgatagctat ttctggaaca gaaaatctga aatgttgttt ttttcacatg aagtgtacct
     3421 gttttcaaaa agcactttat tctatcgttg cttaactatg aactttgcaa tattcttctc
     3481 aaaaacttgt aggacatctt caaaattttg caaggagtga ttagacttgt tcggtaacca
     3541 taaagtgtca tactatgctt atgtatgaaa aagcaatgca actaactcct gagaacttta
     3601 aattactaat tggtgccgaa aaggtagaat ttagaatcga ggtacaccta tggctgtaaa
     3661 atttacaaaa tgagacaact tgggcaagat gtttgaagaa tttcctaaac tccctgattt
     3721 gaagcaagtc actttccctg atgacaaaga aaaaagccaa aacagcaaag aaaaactaga
     3781 tgactgcttt ccaacaactc ccatctagtg tgcttcagac tgggctattt ttctctccat
     3841 ctgttagctt ggattctcag acagtttcag ctaaagaata tcttttccct tatcagaagg
     3901 aacggctcaa accattcaga caagtgaagg gacgacaagc caatatttga aaccagatag
     3961 cagttcttat agtcaattga aataaaatct gaagaaatcg agtaggaaac tcatatcaat
     4021 ttttaacagt gttctattcc agattcaatc cactatacat attaaaaagt atgggtacca
     4081 aaacaaaaaa acaggctctc cgaaaactcg gaaagcctta ttttaagctg tttctagctt
     4141 cctcaccttt acatttcaag gttcgggata aaaggttcaa tggacctttt tatttagcga
     4201 ttgggtaaac agaaacttgt tttttatcgc gtcctttacg ttcaaagcgt actacgcctt
     4261 caactttagc gaacaaagta tcgtctccac cacgaccaac gtttacacct ggatagatgt
     4321 gtgtaccacg ttgacggtaa aggattgatc cacctgttac agtttgtccg tcagctgctt
     4381 tagctccaag acgttttgct tgtgaatcac gtccgtttga tgtagaacct ccaccttttt
     4441 tgtgggcgaa aagttgcaag ttgttaagag tcatttttaa cataatgttt tcctccgtgt
     4501 tagttttctg tgataactct ggtttggacg aactcagaat agttctccga taagtttgcc
     4561 atacctaaga aaaatgattc aaagaataac tgggtcattt ctctctggtg tgaaggaaga
     4621 tcttttggta tttcaaccat cagatagcca ccttcatctt cgtttaattc taggattggt
     4681 tcatagcctg caaatttctc aatagaattg ataaagttaa tggcaagcgt agaaaccgat
     4741 gcacacacga catctaagcc gtattcgcca ctctcggcgt gtccagtaat ttccgcactc
     4801 ctcagctcgc catcttcggc tctctcaaag actgcctgta tcatgtgttc tccttaaaat
     4861 taagcgttga ttgcgttgat gacaactttt gtatatggtt gacggtgacc ttgtttacgg
     4921 tggctacctt ttttaggttt gtacttgtaa gtaaccactt tcttttgttt tccttgtttt
     4981 tcaacagttc caactacagt agctccagca acaagtggag ttccgacaac agtattttca
     5041 ccaccaacaa gaacaacttc gttaaaagta acttcttgac cagcttcaac gttcaatttt
     5101 tcaacgtaaa ctgcttgacc aacttcaact ttaacttgtt ttccgccagt tttgataatt
     5161 gcgtatgtgc tcattatgca cctcctatga tttttatggg tttccccgat tttttgtgaa
     5221 gactcgccta gcatcgtggg acgaaccact taaacttgaa taatcaagca acgatgttcg
     5281 tgcggttgca caggatcgtg catagtcaac tcttcaagta tagcatatct cctattttct
     5341 tacaagtaat aacacctaaa atcaagcttt ttcttttact tttttctgcc aagaggcaaa
     5401 aagcatgctg aggtaaaaaa cgctcatcat aataggaaca ccaagaatgg tcttttcatg
     5461 atagaaaatc gtcaaatagg ctgaaaagac aacgccaagg acaaaactac taagcaggct
     5521 aacaaatatg aatccttcac gcaaaaaagg agtgtgcttg gttcggaaat aatctccaaa
     5581 agccagcatg gtccgtttga tattccctgt cataaaagcg ttattatagg caatacccga
     5641 cacttctcca aaagcagttg tcaccagtcc catacagaag gccaagggcg gcactagata
     5701 gatattatcc acagtttgcg gcacaaaagc aataatgatt gataagattg ccaagggaat
     5761 caaggacaga ataggttttt tcacaattct caatttttcc ttataaatcg ttaataaaaa
     5821 gactcccatc ataaacgcta gcaaggtgag aaccttgtcc ctaacatccg aaacattatt
     5881 tttaattaat tctactgaaa gaaagacaac atttccagtt tgtccagcta caagggtatt
     5941 cccgcgaaca ataaaagtgt aagcatccac atatccagca caaaacgtca aaaaaagtgc
     6001 taacctttta gactgacgtg atatttttct tataggtaat aacctcattt tccctcccat
     6061 tgtattttct cttagaaata ttgtaccatt tctttctaaa aaatcgtaga ctaccattta
     6121 gattttacta ttagcataaa aataataata gacaactatt tatccaaaaa tagatagagg
     6181 taacatgttt gcaaacaaag catacgaatc tttagtaaaa tc
//
LOCUS       DAVHIT010000097         6173 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVHIT010000097 DAVHIT010000000
VERSION     DAVHIT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.099"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1019
                     /locus_tag="VVN16_001841"
     CDS             <1..1019
                     /locus_tag="VVN16_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5573166.1"
                     /translation="LPSRTILLNWLAQYRKNGYTIVEKTRGRVPESGKCHPKKVKRTP
                     IEGGKRERRKTEIVQELMTEFSLDILLKSIKLARSTYYYHLKQLDKTDKDQELKAEIQ
                     SIFIEHKGNYAYRRIYLELRNRGYLVNHKRVQGLIKVLNLQAKMRQKRKYSSHKGDVG
                     KKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLLPVLDGFNSEIIAYNLSTSP
                     NLEQVQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSPDN
                     GMMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRT
                     KSFG"
     gene            complement(1128..1427)
                     /locus_tag="VVN16_001842"
     CDS             complement(1128..1427)
                     /locus_tag="VVN16_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU5573167.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1592..1999)
                     /locus_tag="VVN16_001843"
     CDS             complement(1592..1999)
                     /locus_tag="VVN16_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5573168.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            2125..2767
                     /locus_tag="VVN16_001844"
                     /pseudo
     CDS             2125..2767
                     /locus_tag="VVN16_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2867..3076
                     /locus_tag="VVN16_001845"
                     /pseudo
     CDS             2867..3076
                     /locus_tag="VVN16_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3469..5970
                     /locus_tag="VVN16_001846"
     CDS             3469..5970
                     /locus_tag="VVN16_001846"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU5573169.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAYLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVTTDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYIDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
BASE COUNT         1978 a         1145 c         1270 g         1780 t
ORIGIN      
        1 gtctcccaag tcgtacgata cttcttaact ggctagcaca atacaggaaa aacgggtata
       61 ctattgttga gaaaacaaga gggagagtac ctgagagcgg aaaatgccat cctaaaaaag
      121 ttaagagaac tccgattgaa ggaggaaaaa gagaaagaag aaagacagaa attgttcaag
      181 aattaatgac tgagttttcg ttagatattc ttctaaaatc cattaaacta gctcgttcga
      241 cctactacta tcacttgaaa cagctagata aaacagataa ggaccaagag cttaaagctg
      301 aaattcaatc catttttatc gaacacaagg gaaattatgc ttatcgtcgg atttatttag
      361 aactaagaaa tcgtggttat ctggtaaatc ataaaagagt tcaaggcttg ataaaagtac
      421 tcaatttaca agctaaaatg cgacaaaaac gaaaatattc ttctcataaa ggagacgttg
      481 gtaagaaagc agagaatctc attcaaggcc aatttgaagg ctctaaaaca atggaaaagt
      541 gctacacaga tgtgacagaa tttgccattc cagcaagtac tcaaaagctt tacttattac
      601 cagttttaga tggctttaat agcgaaatta tcgcctataa tctttcaact tcacccaact
      661 tagaacaagt acaaacaatg ttggaacagg cattcaaaga gaagcactac gagaatacga
      721 ttctccatag tgaccaaggc tggcaatatc aacacgattc ttatcatcag tttcttgagg
      781 gtaagggaat tcaagcatcc atgtcacgta agggtaacag cccagacaat ggtatgatgg
      841 agtccttctt tgggattctg aaatcggaaa tgttttacgg ttatgagaag tcgtttcagt
      901 cgcttaagca attggaacaa gccattgtag actatattga ttactacaac aataaacgaa
      961 ttaaggtaaa actaaaagga cttagccctg tgcaatacag aactaaatcc ttcggataaa
     1021 ttaattgtct aactttttgg ggtcagtaca aaactcttgc tactatgcgt tttattattg
     1081 aaagacttat tggactttct ctcaaatcga gtttttactc aattttctta cttgattggg
     1141 attgaaattc caattaattt ctctgagtag agtgtcttga tattggcttc atcaacagag
     1201 gccttatcaa ttttacgttt caagaaaaat tcttgaatgg tttcgatttc aggctcacga
     1261 atagcacggt gtttgtttga gatgaggatt tcatagtgaa gcggagcttg ggtaaaaata
     1321 acatctgtat tccctgcaga ataaacctca acaagggttg catcggtact ttctagttga
     1381 ctttttacaa gttgcgagtg tgagtttgtc gtattgataa gcttcataat atttcctccg
     1441 attttctaat tctattatag cactttttga ataaagtcgc ttgatttata ctcaatgaaa
     1501 atcaaagagc aaactaggaa gctagccgca ggctatactt gagtacggta aggcgacgct
     1561 gacgtggttt gaattttatt ttcgaagagt attagccaat cttatgctgt tttttccaag
     1621 attcaatggc ccatttatgg ctaccacgtt taaggttttg gatagcctcg tcaataggga
     1681 accaggcaat atgattaaag ttttctagtg gcttttgtac ttctttgaaa gaagttgctt
     1741 catagaggta ggcaggattg tagtagtagg tatcacgatg acgagaatag aaatattcgt
     1801 cagcttgtcc gtaataggta ccaatttctg ctgtgaaacc aagctcttca atcaactcac
     1861 gttttagggc ttcctgatga ttttcacctg cttcaatttc tccacatggt aggaaccaag
     1921 caccatttgg ttcttgaaca agaacaattt gtttttgttc aggattaggg ataactgcat
     1981 atacgccata gcgagcaata tagtctgtat tcgctttttt tctccgagag ttgggtttgc
     2041 cattgcattt tcctcattat ctagtatcgt tattattata gtaaaatgaa ccaaaaatag
     2101 tacacaatga ggtataatct tcttatggca tattcaatag attttcgtaa aaaagttctc
     2161 tcttattgtg agcgaatagg tagtataaca gaagcatcac acgttttcca aatctcacgt
     2221 aataccattt atggctggtt aaagctaaaa gagaaaacag gagagttaaa ccaccaagta
     2281 aaaggaacaa aaccaagaaa ggttgataga gatagactta aaaactatct tactgacaat
     2341 ccagacgctt atttgactga aatagcttct gaatttggct gtcatccaac taccatccac
     2401 tatgcgttca aagctatggg ctacactcga aaaaaagaac cacacctact atgaacaaga
     2461 cccagaaaaa gtagccttat ttcttaagaa ttttaataga ttaaagcacc tagcacctgt
     2521 ttagattgac gaaacaggat tcgatactta tttttatcga gaatatggtc gctctttaaa
     2581 aggtcagtta agaagaggca aagtatctgg aagaagatat cagaggattt ctttggttgc
     2641 aggtctaaca aatggtgagt taatcgttcc aatgacttac gaagagacga tgacgagcga
     2701 cttttttgaa gcttggtttc agaagttttt cttaccaaca ttaaccatac catcggttat
     2761 tatagtaaaa tgaaataaga ataagataaa tcgatcagga cagtaaaatc gaattctaac
     2821 aatgttttag aagtagaggt gtactattct agtttcaatc tactatatta tggataatgc
     2881 aagattccat agaatgggga agctagaact tttatgcgaa gagtctgggc ataaactttt
     2941 acctcttcct ccctactcac ctgagtacaa tcctattgag aaaacatggg cttatatcaa
     3001 aaagcacctc aaaaaggtat taccaagttg ccataccttt tacgaggctc ttttgtcctg
     3061 ttcttgtttc aattgactat agcagattga aatgagaaca aatcgattgg gaaagtaaaa
     3121 ttaatttcta taaatgtttt agcaattgtt tcgtactatt tcagattcag tctactatac
     3181 gttcttcata aatcaaaaag cgacataggt agtcggctat ttattgtgaa tacattaatt
     3241 agcattccag ttttatcttc ggtctaaaat aagtattttg tgctatacga gataagcttc
     3301 ttgacttact ccttgattta ctgcataaca atgggataaa aagtgggaga tagagcaatt
     3361 catagtcatc aaaattaatg agatacagta tacagttttt tctttaaaca catttcaaat
     3421 tccctcaaaa atggtataat agtaacatca caaaattgga gagagaccat gagtttttat
     3481 aatcataaag aaattgagcc taagtggcag ggctactggg cagaacatca tacatttaag
     3541 acaggaacag atgcatcaaa acctaagttt tatgcgcttg atatgttccc ttatccgtct
     3601 ggagctggtc tgcacgtagg acacccagaa ggttatactg caaccgatat cctcagtcgt
     3661 tacaaacgtg cgcaaggcta caatgtcctt cacccaatgg gttgggatgc ttttggtttg
     3721 cctgcagagc aatacgctat ggatactggt aatgacccag cagaatttac agcggaaaac
     3781 attgccaact tcaaacgtca gatcaatgcg cttggatttt cttatgactg ggatcgtgaa
     3841 gtcaacacaa cagatccaaa ctactacaag tggactcaat ggattttcac caagctttac
     3901 gaaaaaggct tggcctatga agctgaagtg ccagtaaact gggttgagga attgggaact
     3961 gccattgcca atgaagaagt gcttcctgac ggaacttctg agcgtggagg ctatccagtt
     4021 gtccgaaaac caatgcgcca atggatgctc aaaatcacgg cttacgcaga gcgcttgctc
     4081 aatgacttag atgaactaga ttggtcagag tctatcaagg atatgcaacg caactggatt
     4141 ggtaaatcaa ctggtgccaa tgtaactttc aaagtaaaag gaacagacaa ggaatttaca
     4201 gtctttacta ctcgtccgga cacacttttc ggtgcgactt tcactgtctt ggctcctgaa
     4261 catgaattag tagacgctat cacaagttca gagcaagcag aagctgtagc agactataaa
     4321 caccaagcca gccttaagtc tgacttggtt cgtacagacc ttgctaagga aaaaactggt
     4381 gtttggactg gtgcttatgc catcaaccct gtcaatggta aggaaatgcc aatctggatt
     4441 gcggactatg ttcttgctag ttatggaaca ggtgcggtta tggctgtgcc tgcccacgac
     4501 caacgtgact gggaatttgc caaacaattt gaccttccaa tcgtcgaagt acttgaaggt
     4561 ggaaatgtcg aagaagctgc ctacacagag gatggcctgc atgtcaattc agacttccta
     4621 gatggattga acaaagaaga cgctattgcc aagattgtgg cttacttgga agaaaaaggc
     4681 tgtggtcagg agaaggttac ctaccgtctc cgcgactggc tctttagccg tcaacgttac
     4741 tggggtgaac caattccaat tattcattgg gaagatggaa cttcaacagc tgttcctgaa
     4801 actgaattgc cgcttgtctt gcctgtcacc aaggacatcc gtccttcagg taccggtgaa
     4861 agtccactag ctaacttgac agactggctt gaagtgactc gtgaagatgg tgtcaaaggt
     4921 cgtcgtgaaa ctaacaccat gccacaatgg gctggttcaa gctggtacta ccttcgctat
     4981 attgacccgc acaatactga gaaattggct gatgaggacc tcctcaaaca atggttgcca
     5041 gtagatatct acgtgggtgg tgcggaacat gctgtacttc acttgcttta tgctcgtttc
     5101 tggcataaat tcctctatga cctcggtgtt gttccgacta aggaaccatt ccaaaaactc
     5161 tttaaccaag ggatgatttt gggaacaagc taccgtgacc accgtggtgc tcttgtgaca
     5221 accgacaagg ttgaaaaacg tgatggttcc ttcttccatg tagaaacagg ggaagagttg
     5281 gagcaagcgc cagccaagat gtctaaatcg ctcaagaacg ttgttaaccc agacgatgtg
     5341 gtggaacaat acggtgccga tacccttcgt gtttatgaaa tgtttatggg accactcgat
     5401 gcttcgattg cttggtcaga agaaggtttg gaaggaagcc gtaagttcct tgaccgagtt
     5461 taccgtttga ttacaagtaa agaaatcctt gcggaaaaca atggtgctct tgacaaggct
     5521 tacaacgaaa cagtcaaagc tgttactgag caaattgagt ctctcaaatt caacacagct
     5581 attgcccaac ttatggtctt tgtcaatgct gctaacaagg aagataagct ttatattgac
     5641 tatgccaaag gctttattca attgattgca ccatttgcac ctcacttggc agaagaactc
     5701 tggcaaacag tcgcagaaac aggtgagtca atctcttatg tagcttggcc aacttgggac
     5761 gaaagcaaat tggttgaaga tgaaattgaa attgtcgtcc aaatcaaagg aaaagttcgt
     5821 gccaaactca tggttgctaa agatctatca cgtgaagaat tacaagaaat cgctttagct
     5881 gatgaaaaag tcaaagcaga aattgacggt aaggaaatcg tgaaagtaat tgcggtaccg
     5941 aataaactcg ttaatatcgt cgttaaataa cgagtttatt agctctatct gccaccttca
     6001 atagtccact ggactattga agccaactaa attagttaac attgttgtga aataaaaaga
     6061 agtaactcta gagtatgaac tttagagtta cttttagaat aggtcagaat gaatagatta
     6121 gtgaacgata gcaggaagtg tgaaatttta ttatttaaga tttctattat tgt
//
LOCUS       DAVHIT010000098         6149 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVHIT010000098 DAVHIT010000000
VERSION     DAVHIT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.100"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            333..2666
                     /locus_tag="VVN16_001847"
     CDS             333..2666
                     /locus_tag="VVN16_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5573170.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSIRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2679..4142
                     /locus_tag="VVN16_001848"
     CDS             2679..4142
                     /locus_tag="VVN16_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU5573171.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILDPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4142..5710
                     /locus_tag="VVN16_001849"
     CDS             4142..5710
                     /locus_tag="VVN16_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5573172.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLAEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMP
                     ISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIF
                     PYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIIS
                     KVDLLFQKVSQLFE"
     gene            complement(5658..>6149)
                     /locus_tag="VVN16_001850"
     CDS             complement(5658..>6149)
                     /locus_tag="VVN16_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5573173.1"
                     /translation="SYKKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSE
                     QVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFN
                     LSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQ
                     LWK"
BASE COUNT         2181 a          920 c         1196 g         1852 t
ORIGIN      
        1 gaaaatatta tagcaaaatt tacgccagtt tttgaacggc tgatgtagat attttatact
       61 ttcaaaatgt ttaaatgtga ttatttattt ttgaaaaata gctcaccagc ccgactgaaa
      121 gtgcttatag aatgataata agtcgcctgc cgaaaacagc ggaaaatagc ggtgttatgc
      181 ggagataatc tgacgcgatg cgaaagtata ttgcatactt attttcaaca atttagcaga
      241 gtatttttat aagtgtgata taatagaagt ataatttgtt ctgatagttt attttatgga
      301 gaagtagatt tttagaatgc ggagggttca atatggttga gtttataaag tctaagaaag
      361 aaatgagtga ggaggatatt aaagcaaatt tcatcactcc tgctattgta tccaaaggat
      421 ggaaaaatgg tgagcatatc gcttacgaag aatacttcac tgatggtcga attgaggtta
      481 gaggagataa ggctcgtcgt aaagaaggaa aaaaatcaga ctattcactg tattaccaat
      541 ttggaactcg aattgcaatt gttgaggcaa aggataataa acacagcatt cgagcaggat
      601 tacaacaagc tattgaatat ggagagattt tagatgttcc atttgtttat tcttcaaatg
      661 gtgatggctt tattgaacac gaccgtatca cgagagaaga acgtgagctg gagttagacg
      721 aattccctac tcgtgaagaa ttattttctc gtatgacgaa ggaaaaagga ttgacgtacg
      781 aaattacaga agctatctca actccatact atacagacgc cttctcaatg aaaacgccac
      841 gctattatca gcaaatagct atcaaccgta ctattgaaac agttgccaga ggacaaaaac
      901 gagtaatgtt tgtgatggca acaggaacgg ggaaaacgtt catggctttt caaattattc
      961 atcgccttcg aaaagctggt ttggctaaac gagttttatt cttagcggat agaaacatct
     1021 tagtagacca aacgatggct gaagacttta agccattcga aaaggtaatg acgaaaatta
     1081 caccaaaact tttgactgct cctgaaaaat taaattcttt tgaaatttat ctagggcttt
     1141 atcagcaact aactggtgaa gatggaactg aaacacatta tcaaaaattt gacaaagact
     1201 tctttgattt aatcgtaatt gatgaagcgc accgtggttc agctaaggaa aacagtaact
     1261 ggcgtaagat aattgattat ttcagttctg cgacacagat tgggatgacc gctactccta
     1321 aagaaaccaa gaatgcttcc aatacggaat actttggtga gccaatctat acttatagtt
     1381 taaaacaggg aatcgaggat ggttttttgg ctccatatcg tgttatgagg gttaatttag
     1441 atgtggatgt ggatggttat cgtccagaaa ctggaaaagt tgatactaac ggacaattaa
     1501 tagaagatag gtactacggc aggaaagatt ttgataaaac cattgtcatt gatgatagaa
     1561 cgcaaagagt tgccaagttt gtttctgatt atatgaagca aagcaatgca cgatttgata
     1621 aaacaattgt tttttgtgtt gatattgacc atgccgagcg aatgcgtgct gcacttgtaa
     1681 aagagaatct agacttagtc caagaagact atcgttatgt catgcaagta actggtgaca
     1741 acgctgaagg aaaagctcaa ctggataact ttatggatgt caattctaat tttcccgcta
     1801 ttgtaacaac gtctaaatta ttaacgacag gagttaatgc taaaacatgt cgtttgattg
     1861 ttttagactc taatatccaa tccatgactg aatttaaaca aattattggt cgtggcacac
     1921 gtctttatcc tcaaaagggg aaagaatttt ttacgattat tgattttcga aatgttacca
     1981 atttgtttgc tgaccctgat tttgatggtg atcctgtgaa ggtgctggaa acaggtgcga
     2041 aaacagtcag tggttctacg cccggtttcg tagatgagga aggtgaccca gtagaaaaat
     2101 atatcgttac agacaagcag gttaccattc ttaattctac tgttcaagta ttagatgaaa
     2161 acgggaaact gattaccgaa agcctgaccg actacactcg gaagaatatc ttaggtagct
     2221 acgccacttt gaacgatttt atcacagttt ggcatacggc ggataaaaag aagcttatct
     2281 tagacgaact ttataaaaaa ggagtttatc tagatgctat tcgagagtcg gagggaatat
     2341 cagaacaaga aatcgatgat tttgatttac tcctaaaact tgcctatggt caaaaagaat
     2401 taaccaaaac ggaacgtatc aataaactca aacaaagcgg atatttatat aaatatagtg
     2461 aggaagcgcg tgctgttttg gaaattttac tgaacaaata catggataaa ggtattggag
     2521 aactcgaaag cattgaaaca ttaaaacttc cagaatttca gatatatggt ggaaccttca
     2581 aaatcatcaa tacttatttt ggagataaaa aacgatattt acaagcaatt aaagaattgg
     2641 agcaagagct atttacagta gcttaatgaa aggaaagtat gtcaattaca tcatttgtaa
     2701 aaagaattca agatatcact cgaaacgatg ctggtgttaa tggtgatgct caacgtattg
     2761 agcaaatgtc ttggttatta ttcttaaaaa tttatgatag ccgtgaaatg gtttgggaat
     2821 tagaagaaga cgagtatgag tcaattatcc cagaggaatt aaaatggcga aattgggctc
     2881 atgctcaaaa tggggaacgg gtattgacag gcgatgaatt acttgatttt gtcaataaca
     2941 agttattcaa agagttgaaa gagcttgaaa taacttcaaa tatgcctatt cgaaaaacga
     3001 ttgttaaatc agcttttgaa gatgcgaaca actatatgaa aaatggcgtc ttgttacgcc
     3061 aagtcatcaa tgttattgat gaagttgatt tcaatagccc tgaagatcgt cattcgttta
     3121 atgatattta cgaaaaaatt cttaaagata ttcaaaatgc tgggaactca ggagaatttt
     3181 atacgccacg tgcagcgact gattttattg ccgaagttct taacccaaaa cttggagaat
     3241 caatggcaga ccttgcttgc ggaacaggag gcttcttgac ttcgactctg aaccgtttaa
     3301 gtagtcaacg taaaactagt gaagatacca aaaaatataa tacagctgtt tttggtattg
     3361 aaaagaaagc atttcctcat cttttagcag ttacaaatct gtttcttcac gaaattgatg
     3421 accctaaaat tgttcatgga aatactttgg agaaaaatgt tcgtgaatat acggatgatg
     3481 aaaaatttga cattattatg atgaatccac cttttggagg gtcagaatta gaaacaataa
     3541 aaaataactt tccagcagaa ttacggagtt ctgaaacagc tgatttattt atggctgtca
     3601 ttatgtatcg tttgaaagaa aatggtcgtg ttggagttat tttacctgat ggttttctat
     3661 ttggtgaagg tgtaaaaact cgcttgaaac aaaaactggt agatgagttc aacttgcata
     3721 cgattattag gttgcctcat agtgtctttg caccgtatac aggaatccat acgaacattc
     3781 ttttctttga taaaacaaag aaaacagaag aaacttggtt ttatcgttta gatatgccag
     3841 atggttataa aaatttctcg aaaactaagc cgatgaagtc agaacacttc aatcctgttc
     3901 gtgactggtg ggaaaatcgt gaagagattc tggaaggtaa gttctacaaa tctaaatcat
     3961 ttacacctag tgaattggct gagttgaatt ataatttaga ccagtgtggc tttccaaaag
     4021 aggaagagga aatcttagat ccctttgagt tgattcagaa ttatcaagcg gaaagagcaa
     4081 ctttaaatca taagattgat aatgtattag ctgatatttt gcagttgttg gaggacaaat
     4141 aatgacacca gaacaactta aagcaagtat tctccaaaga gcgatggaag ggaaattagt
     4201 gccgcaaaat cccaatgacg aacctgcaag tgaattatta aagagaatta aagctgaaaa
     4261 agaaaaactt atcagtgaag gaaaaatcaa acgagataaa aaggaaactg agatatttcg
     4321 tggtgatgat gggaaacatt atgggaagtt tgctgatgga agcactcaag aaattgatgt
     4381 tccttatgat attcctgata cttgggagtg ggtgaggttt tctacattgg ttgaaattgt
     4441 cagaggtggc tctccacgac caatcaaaga ttatcttact tctgaagtag atggaataaa
     4501 ttggataaaa ataggtgata ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa
     4561 aatcaaaaaa tcagggctta acaaaactag atttgtaaaa aaaggtacat ttttgttaac
     4621 taattctatg agttttggta gaccttatat tttgaatgtt gatggtgcaa tacacgatgg
     4681 atggttggct atttcgaact atgaaaactc attaaataaa gattacctat tctatattct
     4741 ttcatcaaat gtagtttatt ctcaatttct atctctaatt agtggagctg ttgtgaaaaa
     4801 cttgaatagt gataaagttg cttctattct tatccctctc cctccgctag ctgaacaaca
     4861 acgcataata gaagcaatcg aatcagcttt agaaaaagta gatgaatatg ctgaaagtta
     4921 taatagacta gaacagctag ataaagaatt tccagataaa ctaaaaaaat ctattcttca
     4981 atatgctatg caaggaaaat tagttgaaca agacccaaat gatgaatcag tcgaagtttt
     5041 acttgaaaaa atacgagcag aaaaacaaaa actctttgaa gaaggcaaga ttaaaaagaa
     5101 agatttggac atttctattg tttcccaagg agatgataac tcttattatg ggaacaaaga
     5161 cgaaacaacc tcttatccta tatatgaaat cccagaagca tggagatata ttaaatttgc
     5221 tagcctagtt aacttcagaa taggaaaaac tcctccacgc agtgaagcta ctttttgggg
     5281 aactgaaata ccctgggtat ctatatcaga tatgcctata tctggttatg taactaatac
     5341 gagagaatct atttctaaat tagcattaaa atcaaagaaa atagacattt cacctaaagg
     5401 aactttatta atgagtttta aattatctat tggaaaagtt gccatattgg atattcctgc
     5461 cactcataat gaagccatta tatcgatatt cccctatgct aacaaagaaa atattatcag
     5521 agattactta atgatatttt tgccacttat ctctacttta ggtgattcaa aagatgctat
     5581 caaagggaaa acattaaata gtaccagtat ctccgaatta ttaatcccta tttctaatca
     5641 tgaagagatg aaaagaatca tttccaaagt tgatttactt tttcaaaaag tttctcaact
     5701 ttttgagtaa taagttcctg ttcctcaaaa ggagctaacg gaattaatag ctcgcttagt
     5761 gtagttttag gaatattata taaagcttga cctgatagtt tagttattgc tttcaattgt
     5821 ttataaaata acggagagga caagttaaat aatagaaatt ttgaaataat ctctgaactt
     5881 tcgaatggtg ttaattggaa aataaatcca ccagccacaa caccatcata gtctttatcg
     5941 attcttgcaa actttccaat atgttctaaa gaggttgata caggtgttat tagctgatta
     6001 tgttttaaat aaacttgctc agaggagatg aattgtgtat caatgtagta atcgttatcc
     6061 aacagagaaa attctaaagg cttaatatta ccaccacgta taattctaac acctttatta
     6121 ttaatgctta aatcgccctt cttgtaaga
//
LOCUS       DAVHIT010000099         5895 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVHIT010000099 DAVHIT010000000
VERSION     DAVHIT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.101"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            62..1150
                     /gene="wecB"
                     /locus_tag="VVN16_001851"
     CDS             62..1150
                     /gene="wecB"
                     /locus_tag="VVN16_001851"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU5573174.1"
                     /translation="MKKIMLVFGTRPEAIKMCSLVNELRKQEDMKTVVCVTGQHKEMV
                     SPVLDLFGVQPDYDLEIMKANQNLFSITISILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIANYHFAPTELAKENLIKE
                     GRNNIYVTGNTVIDALTTTVQKDYTHPDLDLNDGNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYDDVKVIYPIHKNPLVRETATEIFGDTERIQIIEPLDVLDFHNFMNHSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKMLLDDPE
                     EYKKMSQASNPYGNGDASKQIVRILRGI"
     gene            1189..2058
                     /gene="rfbA"
                     /locus_tag="VVN16_001852"
     CDS             1189..2058
                     /gene="rfbA"
                     /locus_tag="VVN16_001852"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU5573175.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            2059..2655
                     /locus_tag="VVN16_001853"
     CDS             2059..2655
                     /locus_tag="VVN16_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU5573176.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHRDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            2665..3714
                     /gene="rfbB"
                     /locus_tag="VVN16_001854"
     CDS             2665..3714
                     /gene="rfbB"
                     /locus_tag="VVN16_001854"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU5573177.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKTEKE
                     AVEANYAKTQEIITV"
     gene            3780..4631
                     /gene="rfbD"
                     /locus_tag="VVN16_001855"
     CDS             3780..4631
                     /gene="rfbD"
                     /locus_tag="VVN16_001855"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU5573178.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDIIDAEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFITYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            4694..5791
                     /gene="glf"
                     /locus_tag="VVN16_001856"
                     /pseudo
     CDS             4694..5791
                     /gene="glf"
                     /locus_tag="VVN16_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         1908 a          993 c         1308 g         1686 t
ORIGIN      
        1 attatatatc aatgaaatgg tagattacat ttctaccttt ttatccattt aggaggaaac
       61 gatgaaaaag ataatgctag ttttcggtac acgtccagaa gcaataaaaa tgtgttcatt
      121 agtcaatgag ttgagaaaac aggaagatat gaaaacagtt gtttgtgtaa ctggtcaaca
      181 caaggagatg gttagccctg ttttagattt atttggagtt caaccagact atgatttaga
      241 aattatgaag gctaatcaaa acttgttctc tataacaata agtattttag aaaaaataaa
      301 acctgtgtta gaaaaagaac aaccagatat tgttttggtt cacggtgata ctactacgac
      361 atatgcagca gctttagcgg cattttatct aggaattaaa gttggtcatg ttgaagctgg
      421 attacgaact tacaacctgc aaagtccttt cccagaagag ttcaacagac aatcgacttc
      481 tattattgca aattatcatt ttgctcctac agaattagct aaagaaaatc taataaaaga
      541 gggtagaaat aatatctatg ttactggaaa tacagtgatt gatgcactta caactacagt
      601 acaaaaggat tatacgcacc ccgatctaga tttaaacgat ggaaatcgcc tcatcttatt
      661 gactgctcat agacgcgaaa atctcggaga acctatgaga catatgttta gagctgttaa
      721 acgagtttta aatgaatatg acgatgttaa agtaatttat ccaattcata aaaatccatt
      781 ggtacgggaa acagctacag aaatttttgg agatacagaa cgtattcaaa tcattgaacc
      841 tttagatgtt cttgattttc acaactttat gaatcatagt tatatgattc taactgactc
      901 aggaggggtt caagaagagg caccttcttt aggaaagcct gtattggtca tgcgagatac
      961 gacagaaaga cctgaaggag tagctgctgg aacgttgaaa ttggttggga ctgatgagga
     1021 gactatttat caaaacttta agatgctttt agacgatccc gaagaatata aaaaaatgag
     1081 tcaagctagt aatccttatg gaaatggtga tgctagtaaa cagattgttc gaattttacg
     1141 tggaatttga gtgtgttcag ataaagtaat atagaaaggt accctactat gaaaggtatt
     1201 attctagcag gtggttcggg gacacgttta tatcctttga ctcgcgctgc atcaaaacaa
     1261 cttatgccgg tttatgataa accgatgatt tactacccac tttcaacatt gatgttggct
     1321 gggattaagg atattttgat tatttccact ccacaggatt tacatcgatt ccaagagctt
     1381 cttcaagacg gatctgagtt tgggctcaaa ctttcttatg cagagcaacc aagtccagat
     1441 ggtttggcac aagcctttat cattggggaa gagtttattt ctgatgatag cgttgcgctg
     1501 atcttaggtg acaatatcta tcacggttct gggctttcca agatgctaca aaaggcagcg
     1561 agtaaggagt cgggagcaac tgtttttggc taccatgtca aggatccaga gcgctttggt
     1621 gtggttgagt ttgatcagga tatgaaggct atttctattg aagaaaagcc agagcaacct
     1681 cgttcaaact atgcagttac aggtctctat ttctatgata atgatgtagt agagattgcc
     1741 aagagtatta aaccaagtcc tcgtggtgaa ctggaaatta cagatgtaaa caaggcttac
     1801 ctagatcgtg gtgatttgtc tgttgagctt atgggacgtg gctttgcttg gctggatact
     1861 ggaactcatg aaagtttact agaggcttca cagtacatcg aaacagtgca acggatgcaa
     1921 aatgttcagg tagcaaactt agaagaaatt gcttaccgta tgggctatat cagtcgagaa
     1981 gatgtattgg ccttagccca accacttaag aaaaatgaat acggacagta tctgctccgt
     2041 ttgattggag aagcatagat gacagataat tttttcggta agacgcttgc ggcacgcaag
     2101 gttgaagcta ttccaggcat gttggagttt gatatccccg ttcatagaga taatcgtggc
     2161 tggtttaaag aaaatttcca aaaggaaaaa atgcttccac ttggatttcc agagtctttc
     2221 tttgcagaag gaaaattgca aaacaatgta tccttctcac gtaaaaatgt ccttcgaggt
     2281 ctccacgcag agccttggga taagtacatc tctgtagcag atggagggaa agttctgggt
     2341 tcttgggttg atctacgcga gggtgaaacc tttgggaata cctatcagac agtgattgat
     2401 gcgagtaagg gaatctttgt tcctcgaggc gtagctaatg gcttccaagt tttatcagat
     2461 acagtgtcat atagctatct ggtcaatgat tactgggctc ttgaactcaa acccaagtat
     2521 gcctttgtga actacgctga tccaagcctt ggtattgaat gggaaaatat tgcagaagca
     2581 gaggtttcag aagcagacaa acatcatcca ctacttaagg atgtaaaacc tttgaaaaaa
     2641 gaagatttgg aataaggaaa gaatatgact gaatacaaaa atattatcgt gacaggtgga
     2701 gctggcttta tcggttctaa ctttgtccat tatgtttacg agaactttcc agatgttcat
     2761 gtgacagtcc tagataagtt gacttatgct ggaaaccgcg cgaatattga ggaaatttta
     2821 ggtaatcgtg ttgagttagt tgttggtgac attgctgatg cggagttggt agacaagttg
     2881 gctgctcaag cagatgctat cgttcattat gcagcggaaa gccacaatga taattcgctc
     2941 aatgatccat cgccatttat tcatactaac ttcatcggaa cctatactct tttagaagct
     3001 gctcgtaagt atgatattcg cttccaccat gtatcgacag atgaagttta tggggatctc
     3061 cctttacgcg aagatttgcc aggtcatggt gaaggaccag gtgagaaatt tactgctgaa
     3121 acaaaataca acccaagctc tccgtactca tcaaccaagg cagcctcaga tttgattgtc
     3181 aaagcctggg tgcgttcttt tggagtcaag gcaacgattt ccaactgttc aaataactac
     3241 ggtccttatc aacatatcga aaaattcatc ccacgtcaga ttactaacat cctaagtggt
     3301 atcaagccaa aactttacgg tgaaggtaag aacgttcgtg attggattca taccaatgac
     3361 cattcttcag gagtttggac aatcttgaca aaagggcaaa tcggtgaaac ctacttgatt
     3421 ggggctgatg gtgagaagaa caataaggaa gttttggaac ttatccttaa ggaaatggga
     3481 caagctgcgg atgcctatga tcatgtgact gaccgtgcag gacatgacct tcgctatgcg
     3541 attgatgcta gcaagctccg tgatgagttg gggtggaaac ctgaatttac caactttgaa
     3601 gctgggctca aggcaacaat caagtggtat acagataacc aagaatggtg gaaaacagag
     3661 aaagaagctg ttgaagccaa ttatgctaag actcaggaga ttattacagt ataaaaagca
     3721 ggaaatagct gctttttatt gctatattgg gaagagttac atattagaaa ggtctagaga
     3781 tgattttaat tacaggggca aatggccaat taggaacgga acttcgctat ttattggatg
     3841 aacgtaatga agaatacgtg gcagtagatg tggctgagat ggacattatc gatgcagaaa
     3901 tggttgagaa agtttttgaa gaggtgaaac cgactttagt ctaccactgt gcagcctaca
     3961 ccgctgttga tgcagcagag gatgaaggaa aagagttgga cttcgccatc aatgtgacgg
     4021 ggacaaaaaa tgtcgcaaaa gcatctgaaa agcatggtgc aactctagtt tatatttcta
     4081 cggactatgt ctttgacggt aagaaaccag ttggacaaga gtgggaagtt gatgaccgac
     4141 cagatccaca gacagaatat gggcgtacta agcgtatggg ggaagagtta gttgagaagc
     4201 atgtgtctaa tttctatatt atccgtactg cctgggtatt tggaaattat ggcaaaaact
     4261 tcgtttttac catgcaaaat cttgcgaaaa ctcataagac tttaacagtt gtaaatgacc
     4321 agtacggtcg tccgacttgg actcgtacct tggctgagtt cataacctac ctagctgaaa
     4381 atcgtaagga atttggttat tatcatttgt caaatgatgc gacagaagac acaacatggt
     4441 atgattttgc agttgaaatt ttgaaagata cagatgtcga agtcaagcca gtagattcca
     4501 gtcaatttcc agccaaagct aaacgtccgc taaactcaac gatgagcctg gccaaagcca
     4561 aagctactgg atttgttatt ccaacttggc aagatgcatt gcaagaattt tacaaacaag
     4621 aagtgagata agtagtagaa tgattttcta gtctaataaa agaggcagat aatgaactcc
     4681 aaaggagctt aagatgtacg attatcttgt tgtggtgctg gtctctttgg cgcagtcttt
     4741 gcccatgaag cagccttaaa aggaaaaaaa gtaaaagtta ttgaaaaacg aaatcatatc
     4801 gcgggtaata tctatactcg tgaagaggaa ggaattcaag tttatcaata tggtgctcat
     4861 atcttccata cttctgataa ggagatctgg gattatgtaa atcagtttgc agggtttaac
     4921 cgttacacca attctcctgt tgcaaactat aagggtgaga tttataacct tccttttaat
     4981 atgaatacct tcaataaact ctggggagtt gtgacgccag cagaagcaca agctaagatt
     5041 gatgaacaac gtgctatttt aaatggtaaa actcctgaaa atttggaaga acaggcgatt
     5101 tctcttgtag gtacagacat ctacgaaaaa ttaatcaaag actatacaga gaaacagtgg
     5161 ggcaaaccaa ctactgaact tccagccttt attattgttt gccagtacgc ctgacctatg
     5221 ataacaacta ttttaacgat acctatcaag ggattccaat tggtggatac actcaaatag
     5281 ttgaaaaatg ttggatcatg aaaatattga tgtagaaaca aatgttgatt tctttgtgaa
     5341 taaagagcaa tatctgaaag attttcctaa gattgtcttt actggtatga ttgatgaatt
     5401 ctttgactat aagttgggcg aactagagta ccgtagtctt cgttttgaaa atgagacctt
     5461 ggatatggaa aattaccaag gaaatgcagt tgtgaactat acggatgcag aaatcccata
     5521 tactcgcatt attgaacaca aacattttga gtttgggagt caagcaaaga ctatcattac
     5581 taaagaacat tctaaaacat gggaaaaagg tgatgagcct tattatccag ttaataatga
     5641 tcgtaataat catttgtata aatcgtataa aaaacttgct gatgagcaag ggaatgttat
     5701 ctttggtggc cgcttaggac actatcgtta ttacgatatg caccaagtaa ttggagcagc
     5761 tttgcagtgc gtgagaaatg agttagatta atactcaatg aaaatcaaag agcaaactag
     5821 gaagctagcc acaggttgct caaaatactg ttttgaggtt gcagatggaa gctgacgcgg
     5881 tttgaagaga ttttc
//
LOCUS       DAVHIT010000100         5855 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVHIT010000100 DAVHIT010000000
VERSION     DAVHIT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.102"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..341
                     /locus_tag="VVN16_001857"
                     /pseudo
     CDS             1..341
                     /locus_tag="VVN16_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            508..708
                     /locus_tag="VVN16_001858"
     CDS             508..708
                     /locus_tag="VVN16_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573179.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            733..1149
                     /locus_tag="VVN16_001859"
     CDS             733..1149
                     /locus_tag="VVN16_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573180.1"
                     /translation="MKAALESLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1380..2087
                     /gene="ccdA2"
                     /locus_tag="VVN16_001860"
     CDS             1380..2087
                     /gene="ccdA2"
                     /locus_tag="VVN16_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU5573181.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2098..2655
                     /gene="sdbB"
                     /locus_tag="VVN16_001861"
     CDS             2098..2655
                     /gene="sdbB"
                     /locus_tag="VVN16_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU5573182.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3029..4420
                     /locus_tag="VVN16_001862"
     CDS             3029..4420
                     /locus_tag="VVN16_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5573183.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGIFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4563..5480
                     /gene="adcAII"
                     /locus_tag="VVN16_001863"
     CDS             4563..5480
                     /gene="adcAII"
                     /locus_tag="VVN16_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU5573184.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5488..>5855
                     /locus_tag="VVN16_001864"
     CDS             5488..>5855
                     /locus_tag="VVN16_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU5573185.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEI"
BASE COUNT         1795 a         1033 c         1240 g         1787 t
ORIGIN      
        1 gctatgggct acactcgaaa aaaagaacca cacctactat gaacaagagc cagaaaaagt
       61 agccttattt cttaagaatt ttaatagttt aaagcaccta gcacctgttt agattgacga
      121 aacaggattt gatacttatt tttatcgaga atatggtcgc tctttaaaag gtcagttaag
      181 aagaagatat cagaggattt ctttggttgc aggtctaaca aatgaatggt gagttaatcg
      241 ctccaatgac ttacgaagag acgatgacga gcgacttttt tgaagcatgg tttcagaagt
      301 ttctcttacc aacattaacc acaccatcgg ttattatagt aaaatgaaat aagaacagga
      361 caaatcgatc aggacagtaa aatcgaattc taacaatgtt ttagaagtct atatgtacta
      421 ttctagtttc aatataccat aactgctcct agtcaacctg caagtcctgt cccagctcca
      481 agctcagctc cgactcaaga tcctagagtg gtagcaagcg cttcggctag ctcaacgagc
      541 acacaagctc aagagcaagt tgacaagtct gaacttcgtg ccttgagtca agagttagac
      601 caacgcttga aagctttggc aacagtatct gatccaaaaa tcgatgcaac caaggctgtc
      661 ctcctagatg ctcaaaaagc tccggaagat agtgccttga cagagtaagg tctgcgtact
      721 gcagtagagt ctgtgaaggc tgccttggag tctctcaaag atgtgaaagc aaatgctagc
      781 gatagcaagc ctgcacagga caagaaggat gcaaaacaag gaacggaaga tagtaaggat
      841 tcagataaga tgactgaaac aaactcagtt ccagcaggag tgattgtggt cagtctactt
      901 gccctcctag gcgtgattgc cttctggctg attcgccgta agaaagagtc agaaatccag
      961 caattaagca cggaattgat caaggttcta ggacagctag atgcagaaaa agcggataaa
     1021 aaagtccttg ccaaagccca aaaccttctc caagaaaccc ttgatttcgt gaaagaagaa
     1081 aatggctcag cagagacaga aactaaacta gtagaggagc ttaaagcaat ccttgacaaa
     1141 ctcaagtaaa acatgattta tgaaatgtca gacctaaaag acattttcgt tcacaaaagc
     1201 agcgatgagt aatccttgtc gttgcttttt ggctattttg ggggataaga tccgccttga
     1261 ctagcaaaag aaaagattgg tccaatagtg cccgatacga ttttgcgtag cagaaagctt
     1321 acaaataaaa ttgaatatag taaaatagga ttagaattat taagaaagtt ggttctttat
     1381 tggaaacgat agtattttta atctctgttt ttctagcagg tgttttatcc tttttttctc
     1441 cttgtatttt tcctcttctg ccagtctatg ctgggatttt attggatgat caagaaagtg
     1501 caaaaagctt ttctttgttt gggagaaagg ttctctggtc aggcttgatt cgaacacttt
     1561 gctttatcgc tggtatctct ctcattttct ttattctagg ctttggtgct ggttactttg
     1621 gtcatattct ctatgcaaat tggtttcgat atggcatggg agctattatt atcattttgg
     1681 gtcttcacca gatggaaatt tttcatttga agaaattaga agttcaaaaa agttttacct
     1741 ttaaaaaatc agattctaat cgttattggt cagctttttt acttggtatt acctttagct
     1801 ttggttggac accttgtatt ggtccagttt taagttctgt tttagcactt gcggcttctg
     1861 gaggcaatgg cgcttggcaa ggagcgattt atactctcat ttacactctg ggcatggccc
     1921 ttcctttctt ggtattggca ctagcttcag gtctagtcat gccatatttt agtaaaatca
     1981 agcgtcatat gatgctacta aagaaaattg gtggtttcct cattgtttta atgggaattt
     2041 tgttactatt aggacaagta aatgttctag ctggaatttt tgaataaagg aggacaaatg
     2101 aaaaaagtaa tgtttgctgg cttaagtctc ttgtcattag ttgtattgat ggcctgtggt
     2161 gaggaagaaa ctaaaaagac tcaagcagca caacagccaa aacaacaaac gactgtacaa
     2221 caaattgctg ttggaaaaga tgctccagac ttcacattgc aatccatgga tggcaaagaa
     2281 gttaagttat ctgattttaa gggtaaaaag gtttacttga agttttgggc ttcatggtgt
     2341 ggtccatgca agaaaagtat gccagagttg atggaactag cggcgaaacc agatcgtgat
     2401 ttcgaaattc ttactgtcat tgcaccagga attcaaggtg aaaaaactgt tgagcaattc
     2461 ccacaatggt tccaggaaca aggatataag gatatcccag ttctttatga taccaaagca
     2521 accaccttcc aagcttatca aattcgaagc attcctacag aatatttaat tgatagccaa
     2581 ggaaagattg gaaagattca atttggtgct atcagtaatg cggatgcaga agcagcattt
     2641 aaagaaatga actagaatcg ataaaatctg attacaagat gtagtcagat tttggtaact
     2701 tctaaaacta acttccagtt tcccacaacc tagcttttag tatgagaaaa acgcgtgaca
     2761 tcagtggaaa tccgtcttag actaacttcc actggttttc tttttcttga tatataaggg
     2821 ttcaaaagcg aaaagactca gaaaaagtgc acgacaaata gccctaaaac agagtcgtct
     2881 tagagtaact tccagttttg ggagagagat ggaagttact ttgagaagtt acggtagtca
     2941 gattttttta gaaaaacatt ttataaatat tcacatatct cctatattta tggtaaaata
     3001 gaattatcag tttattttgg agtcaaagat gaatatattt agaacaaaga atgttagttt
     3061 agataaaaca gagatgcata ggcatttgaa gttatgggat ttgattttgc tgggtatcgg
     3121 agccatggta gggacaggcg tctttacaat cacaggtact gcagctgcaa cacttgctgg
     3181 cccagcccta gtgatttcaa tcgttatttc tgccttgtgt gtgggattat cagccctctt
     3241 ttttgcagaa tttgcctcgc gagtacccgc tacaggaggt gcctatagtt acctctatgc
     3301 tatcttagga gaattccctg cctggttggc tggttggtta accatgatgg agttcatgac
     3361 agccatatca ggcgtagctt cgggttgggc agcttatttt aaaggcttgc tctctcaata
     3421 cgggatagcc cttcctcagg ctttaaatgg tacctttaat cctcaagcag ggatatttgt
     3481 tgatttattg cctattcttg tcttggtctt ggtgacttcg cttgttttac taaatgccaa
     3541 ggcagcctta cgctttaatt cgattctagt tattttgaaa ttttccgctt tagctctctt
     3601 tgttttagta ggaatttgga atattaagtt tgataattgg agcaattttg ctccttacgg
     3661 ttttggacaa atctatggag ctagtactgg tattatggct ggtgcgtctt tgatgttctt
     3721 cggttttttg ggatttgaat ccatctctat ggctgtagat gaagtcaaga ctcctcaaaa
     3781 aaatattcct agagggattg tcttatcgct ttctatcgta accattctct atgccttggt
     3841 gacccttgtt ttgactggtg tggttcacta tagtcattta aatgtcgatg atgccgttgc
     3901 ctttgccctt cgtagtgttg ggattagttg ggcagccaac tatgtgtcat tagtggctat
     3961 cttgaccttg attacagttt gtatttcgat gacctatgcc ctatcgcgta tgatttacag
     4021 tttagcacgt gacggcttag tgcctgctgc ctttaaggaa ctgacgaaga ctagcaagat
     4081 accaaagaat gctactatct tgacaggtct agcttcagca gtagcagcag gaatgttccc
     4141 actagccagt attgcagcct ttttaaatat ttgtacctta gcctacttga tcatgttggc
     4201 ttatggtctg attcgcttac ggaaagaaaa aggaatgccc aaagctgggg aatttaaaac
     4261 accattggta cctctgttac caattttatc gatcatcatt tgtttatcct tcatgttaca
     4321 gtataatatg aatacctgga ttgctttcct agttgcgttg ttagtaggaa gtattattta
     4381 cttcacttat ggctataagc attctaccat agaagaataa agtgaaagtc tattgagttg
     4441 ttgacactca gcagattttt atatgtgaaa gaaatttcaa attttttcta aaaatatctt
     4501 gacagattaa attttcagga gtagaatatt tactagttaa ttaaatggtt aaggagttgt
     4561 tcatgaagaa acaaaattta tttttagtcc tgttaagtgt ctttcttttg tgcttggggg
     4621 cttgtggtca aaaggaaagt cagacaggaa aggggatgaa aattgtgacc agtttttatc
     4681 ctatctacgc tatggttaag gaagtatctg gtgacttgaa tgatgttcgg atgattcagt
     4741 caagtagtgg tattcactcc tttgaacctt cggcaaatga tatcgcagcc atctatgatg
     4801 cagatgtctt tgtttaccat tctcatacac tcgaatcttg ggcaggaagt ctggatccaa
     4861 atctaaaaaa atccaaagtg aaggtcttag aggcttctga gggaatgacc ttggaacgtg
     4921 tccctggact agaggatgtg gaagcagggg atggagttga tgaaaaaacg ctctatgacc
     4981 ctcacacatg gctagatcct gaaaaagctg gagaagaagc ccaaattatc gctgataaac
     5041 tttcagaggt ggatagtgag cataaagaga cttatcaaaa aaatgcgcaa gcctttatca
     5101 aaaaagctca ggaattgact aagaaattcc aaccaaaatt tgaaaaagcg actcagaaaa
     5161 catttgtaac acaacataca gccttttctt atctagcgaa gagatttggg cttaatcaac
     5221 ttggtattgc aggtatctct cctgaacaag aaccaagtcc acgacaacta acagaaattc
     5281 aggaatttgt taagacctat aaggttaaaa cgatttttac agaaagtaat gcttcttcaa
     5341 aagtagctga aactcttgtc aaatcaacag gtgtgggtct taaaactctg aatcctttag
     5401 agtcagaccc acaaaatgac aagacctatt tagaaaatct tgaagaaaat atgagtattc
     5461 tagcagaaga attaaagtga ggaaagaatg aaaattaata aaaaatatct agcaggttca
     5521 gtggcagtcc ttgccctaag tgtttgttcc tatgagcttg gacgttacca agctggtcag
     5581 gataagaaag agtctaatcg agttgcttat atagatggtg atcaggctgg tcaaaaggca
     5641 gaaaacttga caccagatga agtcagtaag agggagggga tcaacgccga acaaattgtt
     5701 atcaagatta cggatcaagg ttatgtgacc tctcatggag accattatca ttactataat
     5761 ggcaaggttc cttatgatgc catcatcagt gaagagctcc tcatgaaaga tccgaattat
     5821 cagttgaagg attcagacat tgtcaatgaa atcaa
//
LOCUS       DAVHIT010000101         5462 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVHIT010000101 DAVHIT010000000
VERSION     DAVHIT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.103"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            65..1169
                     /locus_tag="VVN16_001865"
                     /pseudo
     CDS             65..1169
                     /locus_tag="VVN16_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472064.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(1199..3751)
                     /locus_tag="VVN16_001866"
     CDS             complement(1199..3751)
                     /locus_tag="VVN16_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU5573186.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(3813..5435)
                     /locus_tag="VVN16_001867"
     CDS             complement(3813..5435)
                     /locus_tag="VVN16_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5573187.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         1823 a          900 c         1006 g         1733 t
ORIGIN      
        1 aaaggagaaa ctaagtttaa agaatggaaa gcaattttaa aaaaaaccaa cctttattat
       61 tgtcatgatc gggatttctc ttattccaga tctgtacaat atcatatttt tgtcatcaat
      121 gtgggatcca tatgggcaat tgtctgactt acctgtggca gttgtaaata atgataaaga
      181 ggcttcctat aatggtaata ctatggcaat aggaaaagac atggtgtcca atttaaaaga
      241 aaataaaacc ttggattttc attttgtaga tgaagaggaa ggaaagaagg gattggaaga
      301 tggcgattac tatatggtag tgactttacc aagtgattta tctgaaaaaa caactacatt
      361 atccaatatt caatcgacag cagcttatca atcattgaca agtgagcaac aaactgagat
      421 aagtgattct gtatctcaaa atttaactga tagtattcaa tcggctcagt caattgtagc
      481 tttagtacaa gatttacagg gaagtttaga aaacttacaa aatcaatctt ctaatctttc
      541 gactttaaaa aatcaatcta atcaagtatc acctattact tctacttctt tgataggatt
      601 gtcaagtgga ttaacagaga tacaaggaga tgttactagc aaattagttc ctgccagtca
      661 gtcgattgca tcaggtgtaa acgcatatac tacaggtgtt gataaagttt ctcaggacgt
      721 catccagaga gccgttgggc ttggttgaaa tctcgagctg aaataaatgg tattatagct
      781 gttttggcag gaattttggt atatggagga gttcatctta ttggtttaac tgctaatcat
      841 gagatgagaa tatttattct catcatccta acaagtttag tattcatgtc tatggtgacc
      901 actttaacaa cgtggaatag ccgtatagga gctttcttct cacttatttt gcttttacta
      961 cagttagcat caagtgcagg tacttatcca cttgctttga caaatgattt ctttagatct
     1021 attaatccct ggttaccaat gagctattca gtttcgggat tacgacaaac aatctccatc
     1081 aacaagtcat tttcctagct gtcatactag ctctatttac tggtttaggt atgctagcct
     1141 atcaacataa gaaaatggaa gaagattaaa aaaatcgacc gattaactgg tcgatttttt
     1201 atgccttaga tgactttcgt ctgtgattat agattccaaa tagtaagaga gaagtaaagg
     1261 aacagattgc tccagtaata aaaccattgg gaatgaagga aagtgtaata gttcctttcc
     1321 ccttgggaat gtcaactttc ataaatccag tttgagcttg tttaatttct attttcttac
     1381 catcttggta ggcagaccaa cctttgtcat aaggaatggt gaagaaaata gatgtatctt
     1441 gttggacatc atatgtagca aaaaccttgt ttttagaagt tgatactgtg acaggttgtt
     1501 ctttaatttt ttgaattgcc tcggtgaaag ttttggtatc taaacgatag aaggtaggag
     1561 attcaaatga tacttgtgaa tttccaggga aactaacatt gatattgaaa gtttttttct
     1621 ctttagtata tcctagatta aagaaggaga agacattatc agttgtaaaa gtcttttttt
     1681 caccatttac aaggatgtca accttctttt gtttatcgtt agaaaagtga aggtttatga
     1741 aagagagata aacttggctg ttttctggaa cttcaatttg atactggatt gctgcatctt
     1801 catttgaaga acttgtgaca ctaatcaaat cattagtatt ttctattttt tctgtttttt
     1861 cataaggtat tggagaaaaa taatcaaaat tgacgttagc aagttgattt aaaaatgagg
     1921 cctgattatc caaggtatgt tcattgaact tgacatcatt gtaaacagat tgactagcaa
     1981 ctgcaatcgg aagagagtat tgattttcat atagggtaag attatctttt tgatagatat
     2041 ctttaaaacc atacttatca ataggactgt ctgagatatt gtactggata ccaaataaac
     2101 tatcagccaa aatactatta tttgcatatc ggagattgag attagtccca gaggatttaa
     2161 aaccaagttt atctaaagta gagcttgatg aacgatttcg aacagatgaa aattgagaga
     2221 ttccattgta gttgaatttc atactgtcat ttcctgtctg agtttgtagt ttttcagtac
     2281 gagtaaattg atttccaata tatgttgaga aagattccat agctgggata tctcgactat
     2341 aagcacttcg agaagcaaat ccccattcct tagcaattcc gtccatttga gatgaagcat
     2401 ttaaactcat ttcaaccagt ataaataaag agattagaat ggcaaataga ttcacagata
     2461 taaacttttt gataactgca aggagtaaaa gagaatagac aaccaaaaat tcaagagtaa
     2521 gcagaatatt caaatctgtt aaaaaagaat aatgcgattt tagatagatg gtagctaaaa
     2581 atcctgctac tacaagaaaa agcgaaacta aaaaattcca gactttaagt tctttcagac
     2641 gctttaagac ttctgctgct gtgtaaatta acaaggtaga gaaaatccaa gcatagcgat
     2701 gtaaaaacat gtttggagta tgcatgcctt gccaaaataa gtcaagagct tctatgtaaa
     2761 agcttgcaat tagaaatgca aagaatatta catatatgag tttcacgtga aacttaatag
     2821 atttcagcgt aaaaaataaa atggtcaaaa taaagggaag tagtccaaca aaaatcattg
     2881 ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa ttgcttagca aagagatcaa
     2941 gataccagct actttcagtt tgaaactttg taacttcagt caatttttcc ccatgtgtct
     3001 gtaaatcaaa tagagtggga agagtcataa tcaaactagc cataccagct aaaaaggaga
     3061 taactatgaa atcaagaaca gatgattttc gagtcttaaa gtcccacgaa atttgacaga
     3121 gataccagaa aataagaaac aatactgtca tatatccaaa ataataattt tgaataaata
     3181 agattgacag acttgtaaag tacaatagga gtttcttttc agttatcagt agatgtaaac
     3241 cagttataat taaaggaatc aagataaaaa catctagcca ggtttttatc tctaattgac
     3301 tgacagtgaa actcatcaga gcataggaag tagataaggc tagttttaaa gtctgaggga
     3361 tagattgaaa caatttattc aaactaaaaa aggttgacag accaatcaat ccaaatttta
     3421 agagagtggt cagatagaca gcatctggca tattcgttag atcaaaaaag taaactagag
     3481 gcgcgagaaa actacccaag taataactag atagggcata gaagtttagc cctagaccac
     3541 ttgtaaaggt gtaaaacaga ctactatttc catgtaggat atttcgtaag gctacatcaa
     3601 aaataacgta ttgatgaaag ccatctccta atagaggaga gttgtcgcta ttccagtaga
     3661 taccttgaga tagatatact ccagacataa tcactacagg aatgatgaaa gaaataaaat
     3721 aggttcgata tgtttttaaa aatgatttca tgttacctcg tagaatgata gaaaactcag
     3781 ttggttaacc caactgagtt ttgaagtttt atttagtctt tccaaagttc tttaactttt
     3841 gcttgtactt ctgcattttc taggaattca tcgtaggttt catcgatacg gtcaatgacg
     3901 ccatttttag ataagacaat gatatggtta gccaaagttt gaataaactc gtggtcatgg
     3961 ctggcaaaga tgattgattc tttaaagttt ttcaatccat cattcaagct tgagatagat
     4021 tccaagtcca agtgatttgt tggatcatca agtacaagga catttgattt taagagcatg
     4081 agttttgaaa gcatgacacg aactttttct ccccctgaca agacatttac aggtttgtta
     4141 acttcatctc cagagaagag catacggccg aggaagccac gtaggaaagt attgtcatct
     4201 tcttctttac ttgcgaattg acgcaaccag tcaagaattg attctcctcc tgcaaaatca
     4261 gctgagttat cttttggtag gtaagattga ctagttgtaa ctccccactt gacagttcct
     4321 tcatagtcaa tatctcccat gattgcacga attaatgcag tcgtttgaat atcattttgt
     4381 ccaataagtg ctgtcttatc atctggacgc aagatgaaac taatattatc caagatagtt
     4441 tcaccatcaa tctttacagt tagattttct actgtcaaga gatcattacc aatctcacgt
     4501 tccgctttaa agttgataaa tggatattta cgactagatg gcacaatctc ttctagctca
     4561 atcttatcaa gcattttctt acgtgatgtt gcctgccttg acttagaagc attggcagag
     4621 aaacgagcaa caaattcttg caattgttta attttttctt ctgctttagc attacggtct
     4681 gctagcaatt tagcagcaag ctcagaagat tccttccaga agtcgtagtt tccgacatag
     4741 agtttgattt ttccaaagtc aaggtcggcc atgtgagtac aaactttgtt taagaagtga
     4801 cggtcgtggg atactacgat aactgtgtta tcaaagtcaa ttaagaagtc ttctaaccaa
     4861 gtaatcgatt ggatatccaa accgttagta ggctcgtcca agagaagaac atctggttta
     4921 ccaaaaagtg ctttggcgag gagaaccttt actttttcac cgttggccaa ttcgctcatg
     4981 ttttggtagt gtaattcttc tggaatgttt aggttttgaa gtagttgaga ggcttcactc
     5041 tctgcttccc aacctccaag ttcggcaaac tctccttcga gttcggcagc acgaaccccg
     5101 tcctcgtctg agaaatcttc cttcatgtag atagcatctt tctctttcat gatgctataa
     5161 agtttttcat ttcccatgat aacgacatca atggcacgtt catcttcgta gtcaaagtga
     5221 ttttgacgaa gaacagagag acgttcatct ggaccaagag agatgtgacc agtagtaggt
     5281 tcgatatctc cagctaaaat ttttaaaaag gttgattttc cggcaccatt agcaccgatt
     5341 aatccgtaag tatttccttc tgtaaatttg atattgacat catcaaaaag tttgcgatca
     5401 ctaaaacgta gtgaaacatc agatactgta agcaatattt ttctcctata tgtgtaatat
     5461 at
//
LOCUS       DAVHIT010000102         4920 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVHIT010000102 DAVHIT010000000
VERSION     DAVHIT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.104"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            55..1221
                     /locus_tag="VVN16_001868"
     CDS             55..1221
                     /locus_tag="VVN16_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5573188.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            1211..2440
                     /locus_tag="VVN16_001869"
     CDS             1211..2440
                     /locus_tag="VVN16_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5573189.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            2586..3227
                     /locus_tag="VVN16_001870"
     CDS             2586..3227
                     /locus_tag="VVN16_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5573190.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            3237..3866
                     /locus_tag="VVN16_001871"
     CDS             3237..3866
                     /locus_tag="VVN16_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5573191.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            3879..4715
                     /locus_tag="VVN16_001872"
     CDS             3879..4715
                     /locus_tag="VVN16_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5573192.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            4725..4802
                     /locus_tag="VVN16_001873"
                     /pseudo
     CDS             4725..4802
                     /locus_tag="VVN16_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
BASE COUNT         1429 a          807 c         1102 g         1582 t
ORIGIN      
        1 agttattttg tttcaaaaat ggtataatga gagaacaata gaaaggaagt atttatggag
       61 caaaaagaga aacattttag cctatcttgg tttttcaagt ggtttttaga taacaaggca
      121 attacggtat ttttagtaac cttattattg ggattgaatc tttttatttt aagtaagatt
      181 agttttctat tttcacctgt tttagacttt ttagcagttg tgatgttgcc agtcattttg
      241 tctggtttgt tatattattt gttgaatcct attgttgatt ggatggagaa gcataaggtt
      301 aatcgtgtta tagctatcac tattgtcttt gttatcatcg ctctctttat catttggggc
      361 ttggcagtcg ccattccaaa tctgcaacgt caggttttga cctttgcaag aaacgttcct
      421 gtttacttag aagatataga taggattgtt aatggattgg tagcccagca tctgccagat
      481 gatttcagac ctcaattaga gcaagttttg accaattttt ctagccaggc tacagttttg
      541 gcaagtaagg tttcatctca ggcagtcaac tgggtgagtg cctttattag cggggcttct
      601 caagtgattg ttgccttgat tatcgttcct ttcatgctct tttatctctt gcgtgatggg
      661 aaaggcttgc gtaactattt gacccaattc attccaagaa aattgaagga acctgttgga
      721 caagttctat cagatgtgaa tcaacagttg tccaactatg ttcgagggca agtgacagtg
      781 gctattattg tagcagtaat gtttatcatc ttcttcaaga ttattggtct acgctatgcg
      841 gttacgctgg gggttactgc tggtatttta aatctggtcc cttatcttgg tagctttcta
      901 gccatgcttc ctgccctagt attgggtttg attgctggtc cagtcatgct tttgaaagta
      961 gtgattgtct ttattgtaga acaaactatt gaaggccgtt ttgtctctcc attgattttg
     1021 ggaagtcaat taaacatcca ccctattaat gttctctttg ttttgttaac ttcaggatct
     1081 atgtttggta tctggggagt tttacttggt attccagttt atgcctctgc taaggttgtc
     1141 atttcagcca ttttcgaatg gtataaggta gtcagtggtc tatatgaatt agagggtgag
     1201 gaagtcaaga gtgaacaata gtcaacagat gttacaggct ttggaggagc aagatttaac
     1261 taaggctgag cattatttcg ccaaagcttt agaaaatgat tcaagtgatc ttctgtatga
     1321 gttggcaact tatcttgaag ggattggttt ctatcctcag gccaaggaaa tttacctgaa
     1381 aattgtagag gattttccag aggttcatct taatctagct gcaattgcta gcgaggatgg
     1441 tcaaatagaa gaagccttta cctatcttga ggaaatccaa gctgacagtg actggtatgt
     1501 ctcgtctttg gttttgaagg cagaccttta ccagctggaa ggtttgacag atgtggcacg
     1561 tgagaaatta ttggaggcct tgacctactc agaggattct ctcttgatat tgggtttggc
     1621 agagttggat agtgagttgg aaaattacca agcggctatt caagcctatg cccagttaga
     1681 taatcgctcg atttatgagc aaacgggcat ttccacctat caacgaattg gctttgccta
     1741 tgctcagtta gggaaatttg aaacggctac tgagttttta gaaaaagccc tggagttaga
     1801 atacgatgac ttaacagctt ttgagttggc cagtctttat tttgatcaag aagaatatca
     1861 aaaagccacc ctctacttta agcagcttga taccatttct cctgactttg aaggctatga
     1921 gtatgggtac agtcaggctt tacataagga acatcaagtt caagaagccc tgcgtatcgc
     1981 taagcaagga ttagagaaaa atccctttga aactcgcctc ttgctagctg cttcacaatt
     2041 ttcttatgaa ttgcatgatg ctagtggtgc agaaaattat ctccttactg caaaagaaga
     2101 cgctgaggat acagaagaaa tcttgcttcg tttagccact atttatctgg agcaggagcg
     2161 ttatgaggat attctagact tgcagagtga ggagccagaa aatcttttga ccaagtggat
     2221 gattgctcgt tcttatcaag aaatggacga tttggatact gcttatgagc attatcaaga
     2281 gttgacagga gatttgaagg acaatccaga atttctggaa cactatatct atctcttgcg
     2341 tgaattggga cattttgaag aagcaaaagt ccatgctcac acttacttaa aactggttcc
     2401 agatgatgtg caaatgcaag aactgtttga gagattgtaa gaatgtttaa acatatagaa
     2461 ctgtagttta tctcttttga tagctacggt ttttatttgt atatggtaga atctttttac
     2521 aaaaatactt ggtaatcttg tttattcatg ctataatagg aacaattact tttaggaggt
     2581 gcagtatgtc ttatttattt gagatattac cgagtttact gaatggtgcg agcacgactg
     2641 tacaggtctt tgcactggtc ttgctatttt cgattccgtt gggcgttttg attgcctttg
     2701 ccttgcaagt ccattggaag cccctccatt atctgattaa catttacatc tgggttatgc
     2761 gaggaacccc cttactcttg caactgattt ttatctatta tgtgctccca agtattggga
     2821 ttcgtttaga ccgccttcct gcagctatta ttgcctttgt tctcaactat gcagcttact
     2881 ttgcagaaat tttccgtggg ggaattgaca ctattccaag aggacagtat gaggctgcca
     2941 aggtcttgaa gtttagccct tttgacagag tgcgctatat tatcttgccc caagtgacca
     3001 agatcgttct tcctagtgtc tttaatgaag ttatgagttt ggtcaaggat acttctttgg
     3061 tctatgctct cggaatttca gaccttatct tggctagtcg aacagctgct aaccgcgatg
     3121 ctagtctggt tcctatgttc ttggcaggag ccatttactt gattttaatt gggattgtga
     3181 caattatttc caaaaaagtt gagaagaagt atagttatta tagatagggg gcagctatgt
     3241 tagaattacg aaatatcaat aaagtctttg gagacaaaca aatcctgtct gatttcagtc
     3301 taagtattcc tgaaaagcaa atcctggcta tcgttggacc ttctggtgga ggtaagacaa
     3361 ctcttttacg tatgcttgca ggtcttgaaa ccattgattc agggcaaatc ttttataatg
     3421 gacaaccttt agagctggat gaattgcaga agcgcaacct actgggattt gtcttccaag
     3481 attttcaact atttcctcat ctatcagttc tggaaaattt gactttatcg cctgtgaaga
     3541 ccatgggaat gaagcaggaa gaggctgaga agaaggcgag tgggctcttg gaacagttag
     3601 gactaggagg acacgcagag gcctatcctt tctcactatc tggtgggcaa aagcagcggg
     3661 tggctttggc gcgtgctatg atgattgacc cagaaatcat tggctacgat gaaccaactt
     3721 ctgccctgga tccagaatta cgtttggaag tggagaagct aatcttgcaa aatagggaac
     3781 ttgggatgac ccagattgtg gttacccatg atttgcagtt tgctgaaaat atcgcagatg
     3841 tattattgaa agtagaacct aaataggagg aaaaatggat gaaaaaatgg atgcttgtat
     3901 tagtcagtct gatgactgct ttgttcttag tagcttgtgg gaaaaattct agcgaaacta
     3961 gtggagataa ttggtcaaag taccagtcta acaagtctat tactattgga tttgatagta
     4021 cttttgttcc aatgggattt gctcagaaag atggttctta tgcaggattt gatattgatt
     4081 tagctacagc tgtttttgaa aaatacggaa tcacggtaaa ttggcaaccg attgattggg
     4141 atttgaaaga agctgaattg acaaaaggaa cgattgatct gatttggaat ggctattccg
     4201 ctacagacga acgccgtgaa aaggtggctt tcagtaactc atatatgaag aatgagcagg
     4261 tattggttac gaagaaatca tctggtatca cgactgcaaa ggatatgact ggaaagacat
     4321 taggagctca agctggttca tctggttatg cggactttga agcaaatcca gaaattttga
     4381 agaatattgt cgctaataag gaagcgaatc aataccaaac ctttaatgaa gccttgattg
     4441 atttgaaaaa cgatcgaatt gatggtctat tgattgaccg tgtctatgca aactattatt
     4501 tagaagcaga aggtgtttta aacgattata atgtctttac agttggacta gaaacagaag
     4561 cttttgcggt tggatcccgt aaggaagata caaccttggt taagaagata aatgaagctt
     4621 tttctagtct ttacaaggac ggcaagttcc aagaaatcag ccaaaaatgg tttggagaag
     4681 atgtagcaac caaagaagta aaagaaggac agtaagataa accgagaata ttactagaga
     4741 aacaatctaa aaagatagaa gaactacata aaatgataca agatttgatt gaaaaaacat
     4801 aataaaaata aagatgagaa aaagtttgaa ctgaggagaa atcaagtaag ttttgacaca
     4861 atagaacgca tattatcagg tataaaaact tgatactatg cgttttattg tgggaagatg
//
LOCUS       DAVHIT010000103         4844 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVHIT010000103 DAVHIT010000000
VERSION     DAVHIT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.105"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            63..659
                     /locus_tag="VVN16_001874"
                     /pseudo
     CDS             63..659
                     /locus_tag="VVN16_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            735..1184
                     /locus_tag="VVN16_001875"
     CDS             735..1184
                     /locus_tag="VVN16_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5573193.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYKDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1197..2288
                     /locus_tag="VVN16_001876"
     CDS             1197..2288
                     /locus_tag="VVN16_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573194.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLGLMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPDNLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            2278..3923
                     /locus_tag="VVN16_001877"
                     /pseudo
     CDS             2278..3923
                     /locus_tag="VVN16_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4026..4718)
                     /locus_tag="VVN16_001878"
     CDS             complement(4026..4718)
                     /locus_tag="VVN16_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5573195.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1512 a         1035 c          842 g         1455 t
ORIGIN      
        1 ggttttaaaa acgcttacat tgttttaaaa taaacttaaa taacacaacg gaggtatcca
       61 tcatgaaaaa gaaatggatg tattatgctg cttgttcttc taatgaatct gccgatgaca
      121 gttcatctga taaaggagac ggtggttcgc tagtcgttta ttgaccaaac tcagagggct
      181 taattggagc aactattcct gcctttgaag aaaaatatgg tatcaaaata gaactgattc
      241 aagctggtac tggagaactt ttcaaaaaac tagagtcaga aaaagaagtt cctgtagctg
      301 atgttatctt tggtggttct tatacacaat atgctaccca cggagaactc tttgaaaact
      361 atatttcaaa agaaaatgat aatgttatca aagaatatca aaacacaact ggcttctcta
      421 ctccttatac actagatggt agtgttttaa tcgtccaccc tgatttaact aaaggcatga
      481 acatcgaagg atatagcgac cttctcaaac ctgaactaaa aggaaaaatc gcaactgctg
      541 acccagcaaa ctcttctagc gcctttgctc aattaacaaa tatgctacaa gctcaaggtg
      601 gttacaaaga tgataaggct tggtcttatg taaaagatct tttcacactt attgatggta
      661 tagtaaaatg aaataagaac aggacaaatt aatcaggaca gtcaaatcga tttctaacaa
      721 tgttttagaa gtagatgtgt actattctag tttcaatata ctataaaatc ggtttaagtt
      781 catctggtgt ctataaagca gtcgctgatg gagaaatgac tgttggtctc tcttataaag
      841 atccagcagt taaactctta aatgacggag ctaacattaa ggtagtctat ccaaaagaag
      901 gaaccgtctt cctacctgct agtgctgcta tcgttaaaaa atctaaaaat atggaaaatg
      961 ccaagaaatt tatcgatttt attatctctc aagaagtaca agatacactt ggtacaacca
     1021 ctactaaccg tcctgttcgt aaaaatgcta aaacaagcga aaacatgaaa ccaattgaca
     1081 aaatcaaaac actcactgaa gattatgatt atgtcatcaa gaataaatca gatatcgtta
     1141 agaaatacaa cgaagtcttt acagatatcc aatctaaaca gtaaaagagg ttcactatga
     1201 gtgagatcga aattattaac gccaaaaaaa tctaccacga tgtccctgtt attgagaatt
     1261 tgaacattac aattccaaaa ggaagtctct ttacccttct tggagcttca ggatgtggga
     1321 aaacgaccct tcttcgtatg attgcaggtt tcaacagtat caaagatgga gaattttact
     1381 tcgatgatac aaaaatcaat aatatggaac ccagcaaacg caatatcggg ctggttttcc
     1441 aaaactacgc tattttccca catttgactg tccgagacaa cgttgctttt ggtcttatgc
     1501 aaaagaaggt tccaaaagaa gaattgattc aacagaccaa caagtatctt ggactcatgc
     1561 aaattgctca atatgcggat cgaaagcccg ataaactcag tggtggacaa caacaacgtg
     1621 tcaccttggc atgcgcctta gcggttaatc caagtgttct cctcatggac gagccactta
     1681 gtaatctgga ggccaaactt cgcttggata tgcgtcaagc catccgagaa atccaacacg
     1741 aagtgggaat tacaactgtt tatgtaaccc acgaccaaga agaagccatg gctatttcag
     1801 accaaattgc tgttatgaaa gatggggtga tccaacaaat cggccgacca aaagaactct
     1861 atcataaacc agctaatgag tttgtggcaa cctttatcgg acgcacaaat attatccctg
     1921 acaatcttga aaaacggagc gacggcgctt atatcgtctt ttcagatggc tatgcccttc
     1981 gaatgctagc tcttgatcag gttgaggagc aagctattca tgtaagcatt cgtcccgaag
     2041 agtttatcaa agatgaatct ggagatattg aaggaactat tagcgatagc gtctatcttg
     2101 gactaaatac ggagtatttc attgagacag gttttgcctc aaaaattcaa gttagtgaag
     2161 aatcaacttt tgaagaagaa ctacaaaaag gcaatcgtat tcgtctacga atcaatacgc
     2221 aaaaattaaa catcttttct gcagatggtt cccaaaacct gataaaagga gtcaaccatg
     2281 gaacgtaaaa aactaaatat ttggacagcc tcctctttct tcatctttct tacctatctt
     2341 gtctttctcg tttatcctat cgttaccgtg ctcaagtaag cacttataca tgaaggataa
     2401 ttctcactag ctaattttgt cactttcttt agtaaagcct actactctga gacactagtc
     2461 aacagtttca gggtttccat taccgctact gtcacttcct tagttgtagg aaccctatta
     2521 gcttatctct tctctatgta tgacttcaag gggaagaaat ttctacaaat attgattatc
     2581 attgcttcca tgtcagctcc tttcgtagga gcctactcct ggattctctt gctgggacga
     2641 aatgaggtca tcactaaatt tttgacaaat gccctttatc ttccagctat cgatatttat
     2701 ggattcaaag gaattatact tgtctttaca ctgcaactat tcccactggt atttctatat
     2761 gttgctggaa caatgaacag tattgacaat tctctacttg aagcagctga aagcatgggg
     2821 tccttcggat ttaaacctat cgtaacggtt gttttacctc tcctagttcc aaccttacta
     2881 gcagctcctt gcttgtattt atgagagcat tctcagactt tggaacgcct atgttgattg
     2941 gcgaaggata tcggactttc cctgtcctga tttataccca atttattagc gaggttggag
     3001 gaaattctgc ttttgcaatt atggcgatta tcattgcctt ggcaattttc cttatccaaa
     3061 aacacattgc aaaccgctac agtttcagca tgaatctgct ccatccaatt gagcctaaaa
     3121 aaactacaaa aggaaaaatg actgccattt atgcaacagt ctacggaatt atctttatct
     3181 ctgttttacc tcaaatctac ttaatttata cctctttcct aaaaacatca ggtatggtat
     3241 tttttaaagg ttattctcca aacagttaca agctagcttt caatcgtatg ggagctgcta
     3301 ttttcaatac cattcgtatc cctttgattg ccttagttct agttgttcta tttgcgacat
     3361 ttatctccta cctagccgtt agaaaacgga atttgtttac aaacttaatt gacagcctca
     3421 gtatggtacc ttatattgta ccaggaaccg ttctagggat tgccttcatt tcttccttca
     3481 atactggtct atttggaagt ggatttctta tgattacagg gactgctttc atcttgatta
     3541 tgtctctatc tgtcagaaga ttaccgtata ctattcgctc atctgttgct agcttacaac
     3601 aaatagcacc aagtattgaa gaagctgctg aaagcttagg aagtagtcgt ctcaatacct
     3661 ttgctaagat tacaactcca atgatgctat ctggtatcat ttctggagcc atcttatctt
     3721 gggtcacaat gatttcaaaa ctctctactt ctatcctcct ctacaatgtc aaaacaagaa
     3781 caatgactgt agctatttat acagaggttc tcagaggaaa ttacggtgta gccgcagcct
     3841 tgtcaactat cctgactgtt ctaacagtag gttccttgct cttgtttatg aaaatctcta
     3901 aaagcaatag cattacactt tagttttcca catcaaaaac agccgaaagg agtaccctcg
     3961 gctgtttttt cttatcttga tattcttatc aaatccctag ctcatggcaa gcaagtctag
     4021 actgattaaa tagagccttc ttccatcttc tcacgtccta gctctcggta actacggtca
     4081 ccgacaactt caaccctaaa ctgaccgtcc tcatattcaa gaatcgtcac gctaccatta
     4141 tctagaccat gcggatgcat gccattaatc agataaacaa tggttccaat agtcattcca
     4201 tggctgacaa caagggcgtt acctccacct tgatcttcca tttcttttgc aatcatttca
     4261 aagccttcct tgattcggcc actgagtttt tcccagcctt cagcccaacc agctgtatcg
     4321 acctctacca agccctcagc cagttcagca taagacaatt ggtgaacgtg gtccacatta
     4381 aagatacgag gaataatgcc catgaaaaga tcgccatcat aggctccatc aaaactaccg
     4441 aaacaccatt ctctgatacg cttgtccatg cgataaggga ttttcccctg caagccaagt
     4501 tcttcaagga taattcccat ggtctgaatg gtacgaccag aatcactcga ataagcgcgc
     4561 tcaaactgta gatcagattc tcgcaaaccg attcctaact cttgaatccc tcgttcacct
     4621 tcagcagtta agggagtatc gctccaacct tgcgcgcgac caatcgtgtt aaacatggtc
     4681 ttgccatgac gtaccaaata caatcgtact tttaccattt tccgtcctcc gtttgcttct
     4741 attatatcat gaatgataaa ataaaagcca cccatacagg cgacttttga aggagattat
     4801 tatgaaaaag tttaggagtt cattaaataa agatattaga tgaa
//
LOCUS       DAVHIT010000104         4804 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVHIT010000104 DAVHIT010000000
VERSION     DAVHIT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.106"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            12..1823
                     /locus_tag="VVN16_001879"
     CDS             12..1823
                     /locus_tag="VVN16_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU5573196.1"
                     /translation="MISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRL
                     VFILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYV
                     SGEERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLS
                     DFVLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPV
                     QEIDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGL
                     ITHLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAY
                     VSLHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELN
                     QEKLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKI
                     NKFENNGFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREEL
                     SSVVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGL
                     ATVTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            1833..2147
                     /locus_tag="VVN16_001880"
     CDS             1833..2147
                     /locus_tag="VVN16_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU5573197.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            2178..2684
                     /locus_tag="VVN16_001881"
     CDS             2178..2684
                     /locus_tag="VVN16_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573198.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            2764..3927
                     /locus_tag="VVN16_001882"
     CDS             2764..3927
                     /locus_tag="VVN16_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5573199.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQLKAI
                     GRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPIIAQ
                     VPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            complement(4299..4448)
                     /locus_tag="VVN16_001883"
                     /pseudo
     CDS             complement(4299..4448)
                     /locus_tag="VVN16_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1608 a          688 c          924 g         1584 t
ORIGIN      
        1 aaggagtccg gttgatttca aaacaaggtc aagggtatca aatatttttt gagaatcaag
       61 gcgcttacca ggagtttaga caaacttatg aactggaaga agactatacg aaaactgcgg
      121 tttcaaaagg tgatgatcgt ttagtattta tcttaaataa attattattt gagcaagttc
      181 cagttctttt tgatgattta gctgatgaac tttacgttag ccgttcaaca ttatctcatg
      241 atttcagaaa gatacgagtg atgttatcag agtataattt gtccattgag agtagggcga
      301 ataaaggagt ttatgtctca ggcgaggaac gtgataaacg acgttttatc ataaattatt
      361 tcctagagaa tcaatttttt aaaacaatac attgctatgt aaaatttaac ttttttgatc
      421 aaactgttcc attagaagaa tttgctagaa ttgttttgga tgaatgccaa gaggccaacc
      481 taaaactatc tgattttgta ctacagaact tagtagttca catagcattg tctgtgattc
      541 gtttaaaatc cggatttgaa ataaaaaata tagactgtca gatgactgat gatgcaatag
      601 agagaaaggt tgcccaaaga attctttcta aagtaagaga agtaacaaat caggaatttc
      661 cagttcagga aatagactac attactttgc atttgttagc taagagtcaa caatgtcaaa
      721 aaaatcagaa gaatatttct gaagaagttt taaaaaaatc tttatttaaa acatttcaga
      781 atttaggatt agatgatatg tataactttt cttcagattt ccaactaatt gaaggattga
      841 taactcatct aatgacacta caagtacgtt tagaaagccg gattacctta aataaccctt
      901 tggtagatga aattaaacag aattatagtg atatattctt tatgactaga gaaattcttg
      961 caaatatgga caagttcttg gagtggacaa tatcagatga tgaagtagca tatgtaagtc
     1021 ttcacttttt agctgctatg gaacgtagca aagagagtac taagtttaat attcttgcta
     1081 tttgtgcaac aggatttggt gcagcccaaa tgttaagaaa tcgtttggaa acggagtttg
     1141 ggaagcgagt agaagttgtt gatgtaatag gctactatga attgaatcaa gaaaaactta
     1201 aaggaataga ttttatagtg tcagctgttg atttatcaaa tctatatttt caaatcccag
     1261 tttttaaagt tagtgtgttt cttaaaagtg atgagatgga aatgattaga aaagctatgg
     1321 atcagatgca agtttctagt tatgtacaat catcaaagat taacaaattt gaaaacaacg
     1381 gctttagaca gtattttagt aaagaaaatt ttttaatatg tactgaatca gacaaggtaa
     1441 atttacttga aaagatggtt gagagtttat ctgttggtga atcaaatgag tttgaacaat
     1501 ctttacttta tggaattaaa cagcgcgaag aattaagttc agttgttttt tctgaaaaaa
     1561 ttgcagttcc gcatcctatt cagccctttg gtacagaggg aaaggtttcg gttgcaattt
     1621 gtaaagattc gttactctgg gataatcaaa gttcacatgt ccagctagta tttcttctat
     1681 ctccatcaat atatgggaac gaaggtttgg caactgtgac taaaaagata gtctctctga
     1741 cagagaatga tgaattgcaa aatcagctaa tatcttgcaa taactttgaa gattttatta
     1801 acatatttga gaagataaaa taggaggttc atatgaatca agaggaactg caggttgcag
     1861 catttgaaat tatcctgcat tcgggaaatg caaggtcaga aatacatgaa gcatttgcaa
     1921 aaatgagaga aggtagtttt gatgatgcag aaagtaaatt aaatcaatca aatgagataa
     1981 ttcttgaagc gcatcatgca caaaccaaac tcttgcaaga atacgcaagt ggagtggaaa
     2041 ttaaaattga aattatcatg gtacatgctc aagatcattt aatgaccact atgacattat
     2101 tagaagtagc aaaggaaatg ttagctcttt ataagaaagt taactaaata tgtgttgtta
     2161 tataaggagg aaatgatatg gataaaaaag aacgacaaaa gattgaacaa caacgtcgag
     2221 agatggctct aactaatact tttttcaatc gatatcttct tctacgttat tcaatagcac
     2281 tcttcttttt tgggaatatt tattggcttt tgaatcagtt tattagtccc tcgcctatta
     2341 ttattttccc cattatgctc atagtatttt ctattttagc aactgttgaa caatttaaac
     2401 tctatggaaa taggaaagaa aagttgggaa taacactaat gtttgttaga atacaaatgc
     2461 ttatttcaat aggattactt gttttaactt ggacaagttg gtttaagaat ctttttccga
     2521 tttttgaaaa taaccaagta gcacgtttat ttgtttttgt agttcttcta ttaggtttag
     2581 ttctgagttt gttagacatt agaagaatta aaaaaattta taaacgaaca gataaggctt
     2641 atcaacaatt tgtccaattg gaaaagaact cacttagttt ataatcctga tctagtataa
     2701 tttgtatttg aaaccagtct ttagaaagta aaaattatta tgttttatta aggaggtcta
     2761 tttatggatc aacaaaacgg gttgtttggt tttcttgaaa accatgttat gggaccaatg
     2821 ggcaaacttg ctcagtttaa agtagtacgt gctatcacgg ctgcaggtat ggctgctgta
     2881 ccatttacta ttgtaggatc aatgtttttg gtattcagta ttttgccaca agctttctca
     2941 ttttggccaa ttgtggcaga tattttctct gcttcatttg ataaattcac atcactttac
     3001 atggttgcaa actatgcgac tatgggttct ctatctcttt atttcgttct atcacttgca
     3061 tatgaattga caaaaattta tgcagaggaa gaagaactca atatgaatcc tcttaatggt
     3121 gccttgcttg ccttgatggc ttttgtcatg acagtaccgc aaatcatttt tgatggtgga
     3181 atgatgaaga ctgtgacaag tctaaaagaa ggtgcagtaa ttgcagatgg atgggcaatg
     3241 ggaaatggag tcgcacgttt tgggacaaca gggattttta ccgcaatcat tatggcaatt
     3301 gtgactgttc ttatttatcg tatgtgtgtt aaacataatt gggttattaa aatgcctgaa
     3361 gctgttccag aaggagtttc tcgtggattt accgctttgg ttccgggatt tgttgttgca
     3421 tttgttagtc caattgctct tgctaacatg gctgaaaatg ctgctggcgg gcacttcgct
     3481 gttgcaggtg aattttctaa tatgtttgta attgcaggtg gttctggtgc aactttagga
     3541 ctatgtttat atattgcttt tgcctctaaa tctgaacagc ttaaagcaat aggacgagca
     3601 tctgtagttc cagccttatt taatattaat gaaccattaa tttttggatt acctattatc
     3661 tataatccag ccttggctat accatttatt ttagcaccaa tggttactgc tactatttat
     3721 tacgtagcga attctctaaa ctttattaag ccaattatcg cacaggttcc atggccaact
     3781 ccagtaggga ttggagcttt cttagggaca gcagatcttc gagctgtatt agttgctcta
     3841 gtatgtgcat ttgcagcatt cctagtctat cttccattca tccgtgtata tgatcaaaaa
     3901 ttggtgaaag aagagcaagg tatctaataa gtaaaaaagt atactcgatg atagaatcca
     3961 tatagtttaa ataatgagtg ttaatattag cagtgaagag gtctttttaa gatattcttc
     4021 tctgcttttt catatttttt cgctatgtag ggtatgaagc aaaatagaaa gtagttcgaa
     4081 tagttgataa acagggaact gctgttattt taaaaaaaca ataaagtaaa gtatgttatc
     4141 gtagaatacg atacaatagt aaaaaagcaa aaagaatgcg ttttatattt gggtttgagg
     4201 cttataaaat aggataagca tgctgaaaac ttagatatga caaacaaaat aaaattttta
     4261 aaagctacat agaaacatgg tataatgtat ctaaaggcat gattcaaatc aagaatatga
     4321 ttcttaatct ctgttgcccc agaaaggtca ttagagaggg aagctgtgac aggggtgcta
     4381 gtatcactca acatgataca cacatctaat tttgtagagc tcacgtctaa acagacaaaa
     4441 catttcatta aggaaggtct cctttctatt agatttggaa gttttcttga ctattgtaag
     4501 gtttcccaga aacatcttgt tccaacatcc tcgcataaga gaatccttat cacagcctga
     4561 cagagccgcc tgcatcctac tagatgcaga agtcaagctg tggaaacagc tagtgtgttg
     4621 gaagtttgaa caaggcttgg gtaacagact ttttaatgaa gtccaagcaa caaggagaaa
     4681 aaccatgagt ccattgttct agaaaacctt gcaatagtca agtgataaca aataaaactt
     4741 gggattgaaa tatccccatg attatatatt acgaggaggt gtattatgac gcaacgtata
     4801 aaaa
//
LOCUS       DAVHIT010000105         4797 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVHIT010000105 DAVHIT010000000
VERSION     DAVHIT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.107"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..418
                     /locus_tag="VVN16_001884"
     CDS             <1..418
                     /locus_tag="VVN16_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU5573200.1"
                     /translation="KNKVDQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VVN16_001885"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VVN16_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU5573201.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKINSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(3871..4797)
                     /locus_tag="VVN16_001886"
                     /pseudo
     CDS             complement(3871..4797)
                     /locus_tag="VVN16_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         1742 a          778 c          953 g         1324 t
ORIGIN      
        1 taaaaataag gtagaccaag atagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggctccga tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaattcagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtagatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggccga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaagattaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttaacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa attaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 aaagttctct caaaagttag aaataaaact ctgactttgg gagaatttca ttttattagg
     3841 ctctttgtca actgtagtgg gttgaagaaa agctaagctt gagaaaggac aaatttcgtc
     3901 ctttcttttt tgatgttcag agcgatgaaa atccgttttt ttgaagtttt caaagtttcg
     3961 aaaaccaaag gcattgcgct tgataagttt gatgagatta ttggtcgctt ccagtttggc
     4021 attagaatag tgtagttgaa gggcgttgac aatcttttct ttatctttga ggaaggtttt
     4081 aaagacagtc tgaaaaatag gatgaacctg cttaagattg tcctcaataa gcccgaaaaa
     4141 tttctctggt tccttattct ggaagtgaaa cagcaagagt tgatagagtt gatagtggtg
     4201 tttcaagtct tccgaatagc tcaaaagctt gtttaaaatc tctttattgg ttaagtgcat
     4261 acgaaaagta ggacgataaa aatgtttatc gctgagttta cgactatcct gttgaatgag
     4321 tttccagtag cgcttgatag ccttgtattc atgggatttt cgatgaaact gattcatgat
     4381 ttggacacgc acacgactca tagcacggct aagatgttgt acaatgtgaa agcgatccaa
     4441 cacgatttta gcacatggga aaagttgtct agctaagtca tagtaaggac taaacatatc
     4501 catagtaatg attttgacgc gacatcggac aactctatcg tagcgaagaa agtgatttcg
     4561 aatgatagct tgtgttctac cctcaagaac agtgatgata ttgagattgt taaaatcttg
     4621 cgcaatgaag ctcatctttc cctttgtaaa agcatactca tcccaagaca taatctcagg
     4681 aagacaagaa aaatcatgtt taaagtgaaa atcattgagc ttacgaataa cagttgaagt
     4741 tgagatggaa agctgatggg caatatcagt catagaaatc ttttcaatta acttttg
//
LOCUS       DAVHIT010000106         4679 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVHIT010000106 DAVHIT010000000
VERSION     DAVHIT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.108"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            3..185
                     /locus_tag="VVN16_001887"
     CDS             3..185
                     /locus_tag="VVN16_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU5573202.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIASELVAGLR"
     gene            485..1324
                     /locus_tag="VVN16_001888"
     CDS             485..1324
                     /locus_tag="VVN16_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU5573203.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1342..3180
                     /locus_tag="VVN16_001889"
     CDS             1342..3180
                     /locus_tag="VVN16_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU5573204.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVNQGYKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKVYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3193..4608
                     /locus_tag="VVN16_001890"
     CDS             3193..4608
                     /locus_tag="VVN16_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU5573205.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVSERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
BASE COUNT         1592 a          609 c          915 g         1563 t
ORIGIN      
        1 agttgaagag ccagaaaatt aaagtgtctt ttactcaatc aaaaatagta acgcctattc
       61 ttttggtgaa taagactatt gtttggtatg gtgcaatgcc attattaggg aaggtagatg
      121 agatgaccat attacgtttg gaatcagcta gtatagcttc tgaactagtg gcaggtttac
      181 gatagagaaa atttttaaaa atttctatgt atgattttca tttctttagt gagactgttg
      241 tcattatcac attcgaatca cacaaaataa aaaaatttct ataagtactt gacaaataga
      301 ttgaaatatc ttaaaatgaa aatggttaca gagttattaa tgatttaagc ttcatgtcac
      361 cattaaaaat tgaaataaaa ggatgttatc actaatacaa gtgagcagga acctatttaa
      421 tcacatcgga agaagtttct tgatgttttt aagtaggttc cttttatttt aaaagggaaa
      481 ttttatgatc ataaaacgaa tactaaacca caatgccgta attgcgcaaa gtaaaaaaga
      541 tatcgatatt cttctttttg gaaggggaat agcttttgga agaaaaactg gagataaagt
      601 aaatccaatt gatattgaga aaagtttttt tctcaaaaat agagataata tgacccgttt
      661 tacagagatg tttattaacg ttcctttgga gttggtgtac atcaccgaaa aaataattaa
      721 cctaggtaaa ataacattgg gtaataattt tgatgaaatt atctatatta atttaacgga
      781 tcatatttct tcgagcatag aacgttataa agaaggagtt attatttcga atcccctacg
      841 ctgggaaatc tcgaaatatt ataaagaaga atttgaactt gggaaaaggg ctttacaaat
      901 aataaaaaaa gagttaggta ttgaacttcc aattgacgaa gctgcattca tagcgctaca
      961 ttttgttaat gctaatttag aaaataattt tcaagagtcg tataaaatca ctgaaataat
     1021 tatgggaatt gagaaaatca ttcaagattt ctattgtact gagtttaacc aagattctat
     1081 tgattattat agattcataa ctcatataaa attatttgcc catcgcttgg ttgaaaatac
     1141 aacttattgt gacgatgatg atgaagactt gttagcatta atgaaaaata aatatcctag
     1201 agaatatgaa tgtggtgaac aggtggcaat gtttatacaa actgagtaca attatttgct
     1261 gacttctagt gaattagttt atttgatggc tcatattcgt cgcttaacaa aaaatttaga
     1321 ttaatagaat aggagaatac catgagttat aaagatacgg tacaaaaaat cctcgatgta
     1381 attggaggtg aaaaaaatgt caatagagtt acccattgtg taacacgttt aagattagaa
     1441 ttaaaagatg aaaatttagt caatgatgat gatgtgaaga agataccagg tgtaataggt
     1501 attatgaaaa aaaatggaca atatcaaatt atacttggta atgatgtagc taattattat
     1561 aaagaattcg ttaaacttgg caattttgaa tccgattcag ttaatcaagg gtacaaaggg
     1621 aatattttag aaagaatcat tgagtatatc gctggttcca tgactccaat cattccagca
     1681 atgttagggg gaggtatgct gaaagtcttg gtaattattt taccaatgct tggtatattg
     1741 caatcagatt ctcagactat tgcttttttg acattttttg gggatgctcc atattatttc
     1801 ttaccgctgt tattagctta ttctgcatca caaaaattaa aagtaacatc tacattagct
     1861 atgtctgtag caggtgtact tctccatcca aattttgttc aaatggtgca atcagggaat
     1921 cctcttagtt tatttggtgc acctgtgaca ccagctagtt atggttcatc agtcgttcca
     1981 attcttatta tggtttggtt gatgaaatat attgaaaaaa taattgctaa attaacacca
     2041 gctattacta agagtttttt gcaacctacg ctagtattat tagtatcaag ctgtattgcc
     2101 ttagttgtag tcggacctat tggagtaatt gttggtgaag gattatcaaa tctagttggg
     2161 caaatgtatg gtgtagctgg atggcttaca ttagctattc ttggtgctat tatgccattt
     2221 attgttatga ctggaatgca ttgggctttt gcacctattt ttttggcggc atctattgct
     2281 actccagacg tattaattct tccagcaatg ttagggtcaa acttagctca aggggctgct
     2341 tcgatggctg ttgcattaaa gagtaaaaat aataatacaa aacaaattgc ttttgcagca
     2401 ggtttctcag ccttacttgc agggattacc gaacctgcat tatatggtgt gactttaaaa
     2461 tataaaaaac cgctttatgc agctatgatt ggtggtggat tagcgggatt atttgcaggt
     2521 cttactagtg ttaaagtata tctatttgct gtcccatctt tgatagcgtt gcctcaattt
     2581 atttattctg atgtgccatc aaatattgta aatgctttaa ttgtggcggt catttcggtt
     2641 gttattacct ttgtattagc ttatatattt ggaatcgatg aagaagagag ttctagcaat
     2701 ttagaagttg aagctggagt ttcaaataaa aaaatgatat tttctcctat atcaggagaa
     2761 atcattccgt taagcgatgt ccaggataaa acattttcag ataaactaat tggagacgga
     2821 gtagcgatta tcccaagtga aggtaaggtt tatgcaccat ttgatgggaa aattacaaat
     2881 atttttccga ctaagcacgc aattggattg aagagtgatg agggtgttga gttactaatt
     2941 catattggat tagatactgt tgagctaaaa ggtcaaggtt ttattagtca tgtagaagaa
     3001 ggagacagag ttttcaaaaa tcagttgatt tttgaaatgg acttgaattt aatcaagact
     3061 aaaggctacg aaacagttac accagtaatt gtaacgaata ccaatgattt tctagatgta
     3121 ttagtattac ctaataatca gacaatcgag cattctaagg aattactggt aatattataa
     3181 tggaggaaag ttatgaccat ttttccagat gattttcttt ggggtggagc tgttgcagct
     3241 aatcaagtag agggagcata taatgaagat ggtaagggct tatcagttca ggatgtgtta
     3301 cccaaaggtg gattaggaga agcaacagaa aatcctacag aagataactt aaaattgata
     3361 ggtattgatt tttatcataa atataaggaa gatatatcct tgttttctga aatgggcttt
     3421 aatgtttttc gtacttctat tgcatggagt agaatttttc caaaaggaga tgaggaagaa
     3481 cctaacgaag ctgggttgaa atattatgat gaattgtttg atgaactaca tgctcacggg
     3541 atagaaccac ttgtaactct ttcacactat gagactccat tatatttagc aagaaaatat
     3601 catggatgga ttgataggag aatgattcat ttttatgaaa aatttgctcg aacagtttca
     3661 gaaaggtata aagataaagt taaatattgg cttacattta acgaagtaaa ctctgttttg
     3721 gaattaccgt ttactagtgg aggaatagat atacctaagg agaatctttc gaaacaagaa
     3781 ttatatcaag ctatacatca tgaattagtt gcctcgagtt tggttacaaa aattgctcgt
     3841 gagattaatt cagagtttaa ggtggggtgt atggtattag ctatgccagc ttatccaatg
     3901 actccaaatc caaaagatgt atgggcgact catgagtatg agaatctaaa ttatctattt
     3961 tcagatgtgc atgttagagg ttattatccg aattatgcaa aaagatattt taaggaaaat
     4021 gacattaaca tagagtttgc agctgaagat gcagagttat taaaaaatta tactgtagat
     4081 tttttatcct ttagctatta catgagtgtg actcaatctg ctcttccaac acagtataat
     4141 tcaggagaag ggaatattat tggtggttta gtaaatcctt atttagagtc ttctgagtgg
     4201 ggatggcaaa ttgatccaat tggactacgt ataattttaa atagatatta tgaccgttat
     4261 caaattccat tatttatagt ggaaaacgga ttaggtgcta aagatcaact aataaaagat
     4321 gaatttaata acttaacagt ccaagatgat tatagaattc agtatatgaa agaacattta
     4381 ttgcaagtag ctgaagctct acaagatggt gtggaaatta tgggctatac gtcttgggga
     4441 tgtattgact gcgtttctat gtccactgca cagctttcta agaggtacgg tctcatttat
     4501 gttgatcgaa atgatgatgg aaatggcaca ttcaatcgat ataagaaaat gtcctttact
     4561 tggtataaag gagtgattga atcaaatgga gaatccttat tcaaatagtt tgtttattga
     4621 tgattagata ttatcacggt taaggcagca tctcattaaa tatgaaataa agaggtttg
//
LOCUS       DAVHIT010000107         4645 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVHIT010000107 DAVHIT010000000
VERSION     DAVHIT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.109"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1323
                     /locus_tag="VVN16_001891"
     rRNA            <1..1323
                     /locus_tag="VVN16_001891"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1375..1447
                     /locus_tag="VVN16_001892"
     tRNA            1375..1447
                     /locus_tag="VVN16_001892"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:Ala,seq:tgc)
     gene            1572..4473
                     /locus_tag="VVN16_001893"
     rRNA            1572..4473
                     /locus_tag="VVN16_001893"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4550..>4645
                     /gene="rrf"
                     /locus_tag="VVN16_001894"
     rRNA            4550..>4645
                     /gene="rrf"
                     /locus_tag="VVN16_001894"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1288 a          957 c         1378 g         1022 t
ORIGIN      
        1 actaccagat ggacctgcgt tgtattagct agttggtggg gtaacggctc accaaggcga
       61 cgatacatag ccgacctgag agggtgatcg gccacactgg gactgagaca cggcccagac
      121 tcctacggga ggcagcagta gggaatcttc ggcaatggac ggaagtctga ccgagcaacg
      181 ccgcgtgagt gaagaaggtt ttcggatcgt aaagctctgt tgtaagagaa gaacgagtgt
      241 gagagtggaa agttcacact gtgacggtat cttaccagaa agggacggct aactacgtgc
      301 cagcagccgc ggtaatacgt aggtcccgag cgttgtccgg atttattggg cgtaaagcga
      361 gcgcaggcgg ttagataagt ctgaagttaa aggctgtggc ttaaccatag taggctttgg
      421 aaactgttta acttgagtgc aagaggggag agtggaattc catgtgtagc ggtgaaatgc
      481 gtagatatat ggaggaacac cggtggcgaa agcggctctc tggcttgtaa ctgacgctga
      541 ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ctgtaaacga
      601 tgagtgctag gtgttagacc ctttccgggg tttagtgccg tagctaacgc attaagcact
      661 ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa
      721 gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc
      781 cctctgaccg ctctagagat agagttttcc ttcgggacag aggtgacagg tggtgcatgg
      841 ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccctat
      901 tgttagttgc catcatttag ttgggcactc tagcgagact gccggtaata aaccggagga
      961 aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa
     1021 tggctggtac aacgagtcgc aagccggtga cggcaagcta atctcttaaa gccagtctca
     1081 gttcggattg taggctgcaa ctcgcctaca tgaagtcgga atcgctagta atcgcggatc
     1141 agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag
     1201 tttgtaacac ccgaagtcgg tgaggtaacc gtaaggagcc agccgcctaa ggtgggatag
     1261 atgattgggg tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg gatcacctcc
     1321 tttctaagga taaggaactg cgcattggtc ttgtttagtc ttgagaggtc ttgtggggcc
     1381 ttagctcagc tgggagagcg cctgctttgc acgcaggagg tcagcggttc gatcccgcta
     1441 ggctccattg gtgagagatc accaagtaat gcacattgaa aattgaatat ctatatcaaa
     1501 tagtaacaag aaaataaacc gaaaacgctg tagtattaat aagagtttat gactgaaagg
     1561 tcagaaaata aggttaagtt aataagggcg cacggtggat gccttggcac taggagccga
     1621 cgaaggacgt gacaaacgac gatatgcctt gggtagctgt aagtaagcga tgatccaggg
     1681 atttccgaat gggggaaccc aacaggtagt acctgttacc cacatctgtt aaggatgtga
     1741 ggaggaagac gcagtgaact gaaacatcta agtagctgca ggaagagaaa gcaaaagcga
     1801 ttgccttagt agcggcgagc gaaacggcag aagggcaaac cgaagagttt actcttcggg
     1861 gttgtaggac tgcaatgtgg actcaaagat tatagaagaa tgatttggga agatcagcca
     1921 aagagagtaa tagcctcgta tttaaaatag tctttgtacc tagcagtatc ctgagtacgg
     1981 cgggacacgt gaaatcccgt cggaatctgg gaggaccatc tcccaaccct aaatactccc
     2041 tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc gggaggggag
     2101 tgaaatagaa cctgaaaccg tgtgcctaca acaagttcga gcccgttaat gggtgagagc
     2161 gtgccttttg tagaatgaac cggcgagtta cgttatgatg cgaggttaag ttgaagagac
     2221 ggagccgtag ggaaaccgag tctgaacagg gcgaattagt atcatgacgt agacccgaaa
     2281 ccatgtgacc tacccatgag caggttgaag gtgcggtaag acgcactgga ggaccgaacc
     2341 agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc aaacgaactt
     2401 ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgacatta gagattcttg
     2461 gaggtagagc actgtttggg tgaggggtcc atcccggatt accaatctca gataaactcc
     2521 gaatgccaat gaattatggt cggcagtcag actgcgagtg ctaagatccg tagtcgaaag
     2581 ggaaacagcc cagaccacca gctaaggtcc caaaataatt gttaagtgga aaaggatgtg
     2641 gggttgcaca gacaactagg atgttagctt agaagcagct attcattcaa agagtgcgta
     2701 atagctcact agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa caatttaccg
     2761 aagctgtgga tacctttata ggtatggtag gagagcgttc tatgtgtgat gaaggtatac
     2821 cgtgaggagt gctggaacgc atagaagtga gaatgccggt atgagtagcg aaagacaggt
     2881 gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg ccctgggtta
     2941 gtcgggacct aaggagagac cgaaaggtgt atccgatgga caacaggttg atattcctgt
     3001 actagagtat gtagtgatgg agggacgcag taggctaact aaagcagacg attggaagag
     3061 tctgtctaag cagtgaggtg tgaattgagt caaatgctta attctataac attgagctgt
     3121 gatggggagc gaagtttagt agcgaagtta gtgacgtcac actgccaaga aaagcttcta
     3181 gcgtttaaac atactctacc cgtaccgcaa accgacacag gtagtcgagg cgagtagcct
     3241 caggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta acttcgggag
     3301 aaggggtgct gactttaagt cagccgcagt gaataggccc aagcaactgt ttatcaaaaa
     3361 cacagctctc tgctaaatcg taagatgatg tatagggggt gacgcctgcc cggtgctgga
     3421 aggttaagag gagtgcttag cgtaagcgaa ggtatgaatt gaagccccag taaacggcgg
     3481 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac
     3541 gaaaggcgta atgatttggg cactgtctca acgagagact cggtgaaatt ttagtacctg
     3601 tgaagatgca ggttacccgc gacaggacgg aaagacccca tggagcttta ctgcagtttg
     3661 atattgagtg tctgtaccac atgtacagga taggtaggag tctaagagat cgggacgcca
     3721 gtttcgaagg agacgctgtt gggatactac ccttgtgtta tggccactct aacccagata
     3781 ggtgatccct atcggagaca gtgtctgacg ggcagtttga ctggggcggt cgcctcctaa
     3841 aaggtaacgg aggcgcccaa aggttccctc agaatggttg gaaatcattc gcagagtgta
     3901 aaggtataag ggagcttgac tgcgagagct acaactcgag cagggacgaa agtcgggctt
     3961 agtgatccgg tggttccgta tggaagggcc atcgctcaac ggataaaagc taccctgggg
     4021 ataacaggct tatctccccc aagagttcac atcgacgggg aggtttggca cctcgatgtc
     4081 ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg cccattaaag
     4141 cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg
     4201 taggaaattt gagaggatct gctcctagta cgagaggacc agagtggact taccgctggt
     4261 gtaccagttg tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg ataaacgctg
     4321 aaagcatcta agtgtgaaac ccacctcaag atgagatttc ccatgattat atatcagtaa
     4381 gagccctgag agatgatcag gtagataggt tagaagtgga agtgtggcga cacatgtagc
     4441 ggactaatac taatagctcg aggacttatc caaagtaact gagaatatga aagcgaacgg
     4501 ttttcttgaa ttgaatagat attcaatttt gagtaggtat tactcagagt taagtgacga
     4561 tagcctagga gatacacctg tacccatgcc gaacacagaa gttaagccct agaacgccgg
     4621 aagtagttgg gggttgcccc ctgtg
//
LOCUS       DAVHIT010000108         4498 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVHIT010000108 DAVHIT010000000
VERSION     DAVHIT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.110"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(138..1124)
                     /locus_tag="VVN16_001895"
     CDS             complement(138..1124)
                     /locus_tag="VVN16_001895"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU5573206.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1318..3786
                     /gene="gyrA"
                     /locus_tag="VVN16_001896"
     CDS             1318..3786
                     /gene="gyrA"
                     /locus_tag="VVN16_001896"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5573207.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3786..>4498
                     /gene="srtA"
                     /locus_tag="VVN16_001897"
     CDS             3786..>4498
                     /gene="srtA"
                     /locus_tag="VVN16_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU5573208.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTA"
BASE COUNT         1369 a          794 c         1019 g         1316 t
ORIGIN      
        1 gattgaaata agatatgaac aaatcgatta ggaatttaaa gcattttata acaacgtttt
       61 agagtaacgg ggtactattt catataatac aggaaaaaac aactccctga tgattcaagg
      121 agttgtctat agttaaatta gtttttagaa gcttcttgga attctggatt tttccatgct
      181 tcgtcaataa tagcttgcaa ttctttagca gatgcttgca ttttttgagt ttctgcatcg
      241 ttcaatggaa tgtttactgg acgaacgata ccatgtgcac caacaacagc tggttgaccg
      301 ataaagacat tctcaactcc gtattgacct tcttggaata ctgaaagtgg aagtactgcg
      361 ttttcatcgt caaggattgc tttagtgata cgagcaaggg ctactgcgat accgtagtat
      421 gttgcacctt ttttgttgat gattgtgtag gctgcatcac gaacaccttc gaacaattca
      481 atcaattcag cttcttgaac attttgagtg tctttaagaa attcttcaag gtttacacca
      541 gcgatgttag cgtgtgacca aacagcgaac tcagagtcac cgtgttcacc catgatgtag
      601 gcgtgcactg aacgagcatc cacatccaat ttttcagcaa gtgcttgacg gaaacgagct
      661 gagtcaagtg aagtacctga accgataacg cgttctttag ggaaaccaga gaatttccaa
      721 gttgagtaag tcaaaacgtc aactgggtta gcagcaacaa ggaagatacc tttgaaacca
      781 gattcaacaa cttgagttac gattgatttg ttgatagcaa ggtttttacc tacaaggtca
      841 agacgagttt cacctggttt ttgaggtgca cctgcagtga tcacaacaag gtcagcgtct
      901 gcacagtcag agtattgagc tgcatagatt tttttaggtg aagtgaaggc aagggcgtga
      961 ctaaggtcaa gcgcatcacc aacagctttt tcatgcaatt gtggaatttc gataattcca
     1021 agctcttgtg caattccttg gttaacaagt gcaaaagcgt aagatgaacc tacagcacca
     1081 tcaccgacaa ggataacttt tttgtgttgt ttagttgaag tcattgtttt aaacatctcc
     1141 ttaattttat taggggattt tccctagaca acttcattct atcactttta aaaaactttg
     1201 tcacgaatat gccttatagt tctcgatgta aacgttttag tggtttagag gctgaaatag
     1261 atgggaattt atggtataat gttgttactt actaattgtg aaatgaggca tttattaatg
     1321 caggataaaa atttagtgaa tgtcaatctg acaaaggaga tgaaggcaag ttttatcgac
     1381 tacgccatga gtgttatcgt agcgcgagct cttcctgatg ttcgagatgg cttaaaacct
     1441 gttcaccgtc gcattctcta cggaatgaat gaattgggtg tgaccccaga caaaccccat
     1501 aaaaaatctg ctcgtattac aggggatgtc atgggtaaat atcacccaca cggggattcc
     1561 tctatttatg aagccatggt ccgtatggct caatggtgga gctaccgtta catgcttgta
     1621 gatggtcatg ggaattttgg ttccatggat ggagatagtg ctgccgctca acgttatacc
     1681 gaggcacgta tgagcaagat tgctctggaa atgcttcgtg atatcaacaa aaatacagtt
     1741 gatttcgttg ataactatga tgccaatgaa cgggaaccct tggtcttgcc agcgcgtttt
     1801 ccaaaccttt tggttaatgg agcaactggt atcgcggttg ggatggcaac caatattcca
     1861 cctcataatc tgggtgaaac cattgatgca gtgaagttgg tcatggataa tcctgaagtg
     1921 actaccaagg acttgatgga agtcttgcct ggaccagatt ttccaactgg tgctcttgtc
     1981 atggggaaat caggtatcca taaggcttat gaaacaggta aaggttcgat tgtcctacgt
     2041 tctcgtacag agattgaaac gactaagact ggtcgtgagc gtatcgttgt aacagaattt
     2101 ccttacatgg tcaataaaac caaggtgcat gagcatattg ttcgcttggt tcaggaaaaa
     2161 cgcattgagg gtatcacagc agtacgtgat gagtcaaacc gtgaaggtgt tcgatttgtt
     2221 attgaagtca agcgcgacgc ctcagccaat gttattctca ataacctctt caaaatgacc
     2281 caaatgcaaa ccaattttgg tttcaatatg ctcgctatcc aaaatggtat accgaaaatt
     2341 ttgtctcttc gtcagatttt ggatgcttat atcgagcacc aaaaagaagt ggttgttcgt
     2401 cgtacacgtt ttgataagga aaaagcggaa gcgcgcgctc atatcttaga aggtctcttg
     2461 attgcgctag accatatcga cgaagtgatt cgtatcatcc gtgctagtga aacggatgcg
     2521 gaagctcaag ctgagttgat gagcaagttt aagctttctg aacgtcaaag tcaagctatc
     2581 cttgatatgc gtcttcgtcg tttgacaggt ttggaacgcg ataagattca atctgagtat
     2641 gatgacctct tggctctgat tgcggattta gcagatattc ttgctaagcc tgaacgtgtt
     2701 tctcaaatta tcaaagacga attggatgaa gttaaacgta aattttctga taagcgccgt
     2761 acagagttga tgattggaca ggtcttgagt ctcgaggatg aggacttgat tgaagaatcg
     2821 gatgtcttga ttaccctttc taacagaggc tacattaagc gtttggatca ggacgagttc
     2881 actgctcaaa aacgtggggg tcgtggtgtc caaggaacgg gagtgaaaga tgatgacttt
     2941 gttcgtgagt tagtgtcaac tagcacccat gatcatctgc tcttcttcac aaacaaggga
     3001 cgtgtctatc gtcttaaagg ttatgaaatt cctgagtatg gtcggactgc caaagggcta
     3061 ccagtagtca atctcttgaa attggatgaa gacgaaagta ttcagacggt tatcaatgtt
     3121 gagtctgatc gcagtgatga tgcttatctc ttctttacaa cccgtcacgg tattgtgaag
     3181 agaaccagtg ttaaggagtt tgccaatatt cgtcaaaatg gtctcaaagc gctgaattta
     3241 aaggatgaag atgagttaat caatgtcttg ttggcagaag gagatatgga tattatcatt
     3301 ggtaccaagt ttggttatgc agttcgcttt aatcaatcag ccgttcgtgg tatgagtcgt
     3361 atcgccactg gtgtgaaagg tgttaacctt cgtgaaggag acacagttgt tggtgccagc
     3421 ttgattactg atcaagatga ggttcttatt atcacagaaa aaggatatgg taagcgtaca
     3481 gtcgctactg aatacccaac aaaaggtcgt ggtggtaagg gaatgcagac agctaaaatt
     3541 accgaaaaaa atggcttgct ggccggtctt atgactgttc aaggggatga ggatttgatg
     3601 attatcactg atacaggtgt catgattcga accaatcttg ccaatatttc acaaacagga
     3661 cgtgcaacta tgggagttaa agtaatgcgc ctggatcaag atgctcagat agtgactttc
     3721 acaacggttg cggtggcaga aaaagaagaa gttgggacag aaaacgaaac agaaggtgaa
     3781 gcataatgtc tcaaaaaaat aataaaaaga aaaacaagcg aaaaaatctg ctgacaaata
     3841 tcctagcagg atttctgata ttactgtcac tggctttgat ttttaatact caaattcgaa
     3901 atattttcat agtctggaat accaataagt atcaagttag ccaggtatca aaagaaaaat
     3961 tagaagaaaa tcaggataca gaaggcaatt ttgactttga ttctgtcaaa gctatctctt
     4021 cggaagctgt tctaacttct caatgggatg ctcaaaaatt accagttatt gggggaattg
     4081 caattcctga attggaaatg aatttgccga tttttaaagg acttgataat gttaatctct
     4141 tctacggagc tggtacaatg aaacgcgagc aagtaatggg agaaggaaat tatagtctag
     4201 ctagtcacca tatctttggt gttgataatg ctaataaaat gttattttct cctttagata
     4261 atgctaaaaa tggcatgaag atttatctaa ccgataaaaa taaagtttat acttatgaaa
     4321 tacgtgaagt caaacgtgtg acaccggatc gtgttgatga agttgatgat agagatgggg
     4381 tcaatgaaat cacattagta acctgtgaag accttgctgc tacagaacgt attattgtca
     4441 aaggtgattt gaaagaaaca aaagattatt cacaaacatc taatgaaatc ctaacagc
//
LOCUS       DAVHIT010000109         4485 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVHIT010000109 DAVHIT010000000
VERSION     DAVHIT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.111"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(358..909)
                     /locus_tag="VVN16_001898"
     CDS             complement(358..909)
                     /locus_tag="VVN16_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573209.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKESPEQVIGDVSIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1259..2083)
                     /gene="nadE"
                     /locus_tag="VVN16_001899"
     CDS             complement(1259..2083)
                     /gene="nadE"
                     /locus_tag="VVN16_001899"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU5573210.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2080..3540)
                     /locus_tag="VVN16_001900"
     CDS             complement(2080..3540)
                     /locus_tag="VVN16_001900"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5573211.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMHNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3656..4144)
                     /locus_tag="VVN16_001901"
     CDS             complement(3656..4144)
                     /locus_tag="VVN16_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573212.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4134..4343)
                     /locus_tag="VVN16_001902"
     CDS             complement(4134..4343)
                     /locus_tag="VVN16_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5573213.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1289 a         1051 c          832 g         1313 t
ORIGIN      
        1 catcagaatg agactatttt aatattagat attttgatag aaaaaaacat aaataaaatt
       61 ggttgaattt aattccctaa ctcgatagag gattctattt taaatttaac aacagctagg
      121 attacactat aaaaagcaca ttaccacctg ttcggctgga gaatctagtc gattagctcc
      181 ctcttagtta caagcttcaa gcgatttttt aactaatact aaaccagatt aaagaagaaa
      241 ctagaacatc tgtcttgtac taatcaatat gctattttaa agttgcaaat aaacttggaa
      301 tgctaattat tatatttaca ataaatagtt gacttcgata gaaacagaaa atagaattca
      361 acattcttcc ctacttatac cataataaat aagatccgca agatagcctt ttctctctac
      421 accattaaca atggtttgta gataggacat tccagccttt tccatgacac gacctgaagc
      481 tgggttgaga ctggcataac gtgctctgac cttttgaaaa cctgcttgag taaaacaaaa
      541 gtccaagaca gctttcaagg cctctgtcat cataccatgt ccccagtaag ccttgcctag
      601 cacatagcca atttcacagc ttaaatcagc ctcgtctatc ttaacaatgc tgacatctcc
      661 tatcacttgc tctgggcttt cttttagaca aatggcccat ttgtaatagt tgagattagt
      721 ataggaagca acccaattgc gaatcgagtt tcgagtgatt tcgacatcag gatggggatc
      781 ccaggtaaca taggtcaaat tctcagcgga tgaagcccaa ttttgaaaca tggcttctgc
      841 atcactctcc acaaatcttc ttaaaatcaa acgttctgtc tgtaatattt gcgtaccgat
      901 agatttcatg atgctacctc acttccttat ttctatgctc gtagtaaaaa gactcaactc
      961 agtaacagct agaaaacgag tttgtcttct aatttcttga cgcagtcgct gcctggtttg
     1021 aaatgcgctc tagcaagctt tttcaaacct agtcatcgtt gcaggggtaa gacgatgaaa
     1081 tcgaactctt cgagttactg aatctgcctc attttcttat ttctaaactc ggggttcaag
     1141 atcccccaga ccttctctca ctttcttatt tcaagatgag aggctgaaaa cctccagtgg
     1201 aggtttttct tgctttttaa tttcagggca agaagctaaa aaggtccccc ggaccttttt
     1261 actcccaaaa gtcatcaaat acggtgatgg gtaagtggcg tttgtgttgg cctttgtgcc
     1321 accagttttc aatggtcgct tgagcttctg ggctgattgt tttgccttct aggtagtcgt
     1381 caatctctgc gtaggtgact ccaagtgcga cttcgtcagc taggcctggt ttatcttctt
     1441 ctaggtctgc cgttgggatt ttttcataaa gggctggctc tgcgccaagt ttctgcaaga
     1501 gctgttttcc ttggcgttta ttgaggcggt aaagagggag aatatccgca ccgccgtcac
     1561 caaacttggt aaagaaacct gtgatatttt ccgcggcgtg gtctgttcca atgaccgctc
     1621 cgctatggga accagcaagg gcatactgag caatcatacg gcaacgtgcc ttgatattcc
     1681 ccttgttgaa gtctgaaaca ggactacctg tcgcttcaac tgcagctgtc atggcatcag
     1741 ctgattcctt gatattcaca accaagctga catctggctg gatgaaggct agggcttttt
     1801 gagcatctgc ttcatcagct tgcactccgt atggcaggcg gacagcgata aatttgtagc
     1861 tatcgtctcc cgtttcagct cgcagttctt ccatagctaa ttgcgccaaa cgtcctgcca
     1921 aggttgagtc ttgtccccca gaaatcccta gtacaaaggt ttttaggaag ggatgttttt
     1981 tcagatatct ttttaagaaa tcaatagaac gacggatttc ttcctgggca tcaatcactg
     2041 gtttgacacc cagctcttgg ataatcgttt cttgcaaact cattcttctt ctccttcacc
     2101 aagggcttcc ttgcgcatct tatcaatcaa gtccatctta tcttgccata catcacgcgc
     2161 caaatccact ggatagtgct gcggattgag cacacgctta tactcatccc acaacttgtc
     2221 aaattcctta cgggcataat cctgaatgtc agtcaaacta ggcaagttgt aaactaatat
     2281 tccttctttg aagatatcca ccaagagagg aacggcatca aaattacgaa ccgtcttctt
     2341 gatgtatgta taggtcggat ggaacatctt gatttctgtc atgtcgctaa tatccacacc
     2401 atcataagtg atgtagtcac cttctgactt gcctttttca cgactggtaa tgcgccacac
     2461 ctgcttctta cctggcgtcg acactttttc cgcattatta gacagcttaa tcgtattgtg
     2521 catctgacca gtttcatctt caattgcaac aatcttgtaa accgccccaa gagccggctg
     2581 gtcataggct gtaatcagct tggtacccac accccagaca tcaatcttgg ccttttgcat
     2641 cttgaggtta aggatggtat tttcatctag atcattagaa gcataaatct tagcctctgt
     2701 aaatccagcc tcgtccagtt gctgacggac tttcttagaa atgtaggcaa tatccccaga
     2761 gtcaatccgc acacccataa agttaatctg atcacccagc tcacgcgcca cctgaatggc
     2821 agctggtaca ccgatgcgaa gggtatcata ggtatccaca agaaagacac aatttttgtg
     2881 ggtcgcagcg taagccttga aagcctcata gtcattgcca taaacctgta ccaaggcatg
     2941 ggcatgggtt cccaaaacag gaatgtcaaa gagcttaccc gcacgcacgt tgctggttcc
     3001 attggcgcca ccaatcaccg ctgcgcgtgt tccccagatg gccgcatcca tttcttgagc
     3061 ccgacgtgtc ccaaactcca tcaagggttc atcttcgata accgaacgaa tacgagctgc
     3121 cttcgtcgcc accaaggtct ggtagttgac gatgttcaaa agagccgttt cgaccaactg
     3181 acattgggct agaggtcctt ccacctgcac aatcggttca ttagcaaaaa ccaaatcccc
     3241 ttcttgggca gaacgaacgg tcaactccaa cttgaaattg cgaaggtaat ccaagaacgc
     3301 cccatgataa ccaagcgact ccaaataggc tatatcacta tctgaaaaac gcaagtcttc
     3361 aagatagttc acaattcttt ccaaacctgc aaaaaccgca tagccgttct taaaaggctg
     3421 ttggcggaaa tacacctcaa agaccgcctt cttattgtga atcccttggt caaagtaaac
     3481 ctgcatcatg ttgatctggt acaagtccgt gtgcaatgtc aaactatcat ctggatacat
     3541 acttttccta cttccttagc tagaaaccca tgaaaatttt caagaacttt catgtattcc
     3601 aataaattag tactattata tcacatttta gctggattga gaaaagagta acaagctatt
     3661 ctccactctc caattcatcc atatcttgtt caaatttttt ctgagcccat tcgccatagc
     3721 tcttaagacc aagattgcca ataaagaccc acggaaggta aatgacataa gtaatgaccc
     3781 aagcagacag gtatttaaaa ttcaaaggat tgtgctgata aatttctatg ttgaattgat
     3841 aattctgcaa catcaaaaga gccgtaatag ccaaggttag gaaaaaacaa cccaaaatcg
     3901 taaaatgaaa acgactatag taggtcactc ccagataacg ggcacggtta aaaaagtaaa
     3961 atgtccctat gatgataacg attagcagca tattagaatt aaaaaggctt ggtgctaata
     4021 ctgaaatgat ataagatagg agcgacaaag cgatgcagat atagaaactt tcagagcccg
     4081 ctttattgat cagttgttct tctctttcgt ctagtaattg ataataataa aatctatttt
     4141 tcatcttctt cctcccaaaa tagttggtct agggttttcc ctaaacatct gcaaatagac
     4201 tggcagagcg agagactggg attgtatttt cccgcctcta tcaaaccaat agtctggcgt
     4261 gtcaccccga cagcctctgc cagttgacct tgtgttaaat cacgctctac ccgagctaat
     4321 tttaatttta aatttttagc caccttcgtc ctccttatag ttttaatact catctactct
     4381 taaaaaatcc aaaaccaaca caagctatca gctatttatt ataaccccat taatttgcac
     4441 tccagtatta tcttcagccg aaaatcagta ttttaggttg tttga
//
LOCUS       DAVHIT010000110         4316 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVHIT010000110 DAVHIT010000000
VERSION     DAVHIT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.112"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(285..1511)
                     /locus_tag="VVN16_001903"
     CDS             complement(285..1511)
                     /locus_tag="VVN16_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5573214.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1583..2248)
                     /locus_tag="VVN16_001904"
     CDS             complement(1583..2248)
                     /locus_tag="VVN16_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5573215.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFVVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2316..2513)
                     /locus_tag="VVN16_001905"
     CDS             complement(2316..2513)
                     /locus_tag="VVN16_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU5573216.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            complement(2804..3667)
                     /locus_tag="VVN16_001906"
     CDS             complement(2804..3667)
                     /locus_tag="VVN16_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU5573217.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
     gene            complement(4062..4310)
                     /locus_tag="VVN16_001907"
     CDS             complement(4062..4310)
                     /locus_tag="VVN16_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU5573218.1"
                     /translation="MKYNSDNYKESAILSIINELLIVGIKILVYEPNLNVSIDNVIIE
                     KNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
BASE COUNT         1499 a          816 c          754 g         1247 t
ORIGIN      
        1 gcaaagtgaa gctgacgtgg tttgaagaga gtttcgaaga gtataagtag gctgaaaaga
       61 atccagccac agcatggact attatatagc agattgaaat aagatgagaa caaatcgatt
      121 gggaaagtaa aattaatttc tataaatgtt ttagcaattg tttcgtacta ttttagattc
      181 agtctattat aacacattca gaaaagagaa aaaagtctgt tgattttgac catcataaaa
      241 agactggcaa tccagtctca aacatatatt atagaaattc tccactaaat actttcacga
      301 atattcagaa gcataacaaa ggcaactaga agaaatagca ataaaacaaa gctaactgcc
      361 agagttccaa agctagtagc aatggttacc aaagctattg taaataagct aggtaaaaca
      421 accgtaatgg caccgataga ggattgaact gctcccattg actcctcagg tatttgttta
      481 aagacgagtt cttgcaatct aggagagaga acacctgcga aaaaggcatc caaggtacta
      541 aagatgagaa tccagtcaaa acgaactgtg gcaaatccta ctagaagaag caactggatg
      601 acaagtgagg catagagagc tgtttttatg gaaatggtat gttgcagata gccacttaca
      661 aggcttccga caatcagggc tgacagttca aaagtggata gcaaggcaag agattgacct
      721 gtttgtaaat tcaaaaaggg ttgattcctc aaaaatagag tagagacagg aactgtaaca
      781 tttatcactg cttgactagt agagataata aacaaaacca agagcacctt attcatattc
      841 catatcaatt tcgatgattg gagcaaatgc tggcaaaagg attttacaga gagtccttct
      901 tgatagttaa tcgttttttc tactttcaag aggtcagttt ttatgaagag gatacctaaa
      961 aatgcgatta aaaaggtaag agcgttcagc aaggaaatga actggatgga gagaatccct
     1021 agcaagactc ctcctagaat attactgatt gttttcacta aactaacagt tgactgttta
     1081 aatccaatag cttctgccag atggtcttgc ccaataattc taatgaaaat cggagtgagc
     1141 atggctcctg aaaagtaact caatgtgtca gacaagaggt taatcagaca aataaaggtt
     1201 actagcaaca aggaaaaaga ctgccctgaa agcgataggg acactataga gtaaagcaaa
     1261 aattttgtaa aactaatgac tgtgtatttc aagacacgat gatgttgaaa atccgccaaa
     1321 actcccagaa agatttgtag aacttgggga agggtttctg aaatcgtgat aagtaaaatc
     1381 gccaaagggg caaaagacgc atctgccaca taattcagga aggccagata aaaaatcgta
     1441 tccccaagcg ttgaaatcca ctggttgata gttaattgcc taaaatctct attttgaaga
     1501 aatactttca ttacaactcc tttttaaatt caaatgggaa tctttcccca agaatagacc
     1561 gcgatactac taacaaccaa aattacagta acatcaaaag ctgaccaatg ccgttgtaga
     1621 ctatatgcag cccaataggc caataaatgg actttgtcat tctaaataag actacaaata
     1681 taagtccgcc acccatatag aagacaaagt ctgtcaagac ccaaccgtga ttactaatgt
     1741 gcgagacccc aaataaaata gcagaaccaa gtacatctag cccccatttc tttccttttt
     1801 ccagagctgt catcaccaat ccccgatgga ttatctcttc aaaaatggga cctgcaatca
     1861 caggatagaa aaaatacatc aaaaatgctg tagcccctgt aaaagtcgga gcagcatgtt
     1921 gataagaaat ttcatttcga gtaggtggga aaagaaaaaa ggtaacgaaa ttccaaacaa
     1981 caaaagcaag cagagctagg aaggaataga aaagatagga tcctttaaac tttctactat
     2041 tgattttctg ccatttcccc gaccaaatca tagcaataag agcaaataaa accacaagaa
     2101 aattcaacat catatccgac agataatagg caaagtcaga tagcccagta acaaggtcgc
     2161 tgcgtaaaac tagaacactg aacttctggt cagcaataac tagtagaaaa actataataa
     2221 agtagcggtg tgagattatc tttttcattc tattttctcc tatactatga aataaagaca
     2281 gtccctaccc aagaaaaagg caaggactct gaatatcaat aatggcgacg attaacagtg
     2341 gaagaatcat ggttaaaata tttcttccag aaattagtga ttgaccaaga cgttcctaaa
     2401 cctccccaag caccggctgg gataacagca ttattcccaa attcccctcc ataaactgtc
     2461 tccaactcag cttctgtcaa ttccattgtt tctgcaaatt gtaaatttaa catcttttac
     2521 actccttcaa ttatcttcat ttgtaaacca cttctgcgac ctaggatttg cttcaagtgc
     2581 tttacaagta cagtataaca cgaaaattgg cttattttaa aaaatcgcat atttgatatt
     2641 ttttcttata gaaatttctt atttgcgatt ttgatagatt tgattatttc cctggtataa
     2701 taaagttatt actaacaagg aggaatataa tagatgaaga aaagaaaaat ccaaccgata
     2761 ctcctactta tttcggagtg ggttattgtc attccatttt taactaatct ataagttctt
     2821 tatattgctg aaaacgcaat tcaaaaaggg atgttaattg tggattttct aatacctgca
     2881 gagattggat aaagcgttca atctcttttt gattgcttcc ctttgtttga agaaagacac
     2941 tcatcttctt taaaaattgc cacgatactt tttcaaaaac atcatacggt cgtaacatcc
     3001 tctccaactc ggcttcgaag attgggatgt aggagaaaag ttttcgctcc atgagttctg
     3061 ataagatatt taagagtcct tgcttcatat acaatcgatt gtgtactaac tctttaaatt
     3121 ctttggattt ttcgagtaag gaggttgata aaaaaatcag atcttgattg ctcaagaagg
     3181 gcatggtatt gcaaaagaga tagagttcaa accaggtcca agactcgata gcatagagat
     3241 aggtggtcaa aaactcgcta tcctcctctg ctagtgggta gcttttattt agtgaatgga
     3301 tggcatcttt aatcacgatg gcattcaaac gacgataggt ctgcgccatc tgttcttgat
     3361 cgacttcctc caatagctgc tctaaagcag ctatatcctg atgggcaaag cgattcacaa
     3421 cctttcgacc gattcgcata tgtggagatt cttgatagtt gttgagcttg tgcccaaact
     3481 catcaaaggt cacatttata ccttggatag ctagaatcaa cttatccgca gacagcatag
     3541 actgccctag ttcaaacttg gacaactgag aagctgttag accctcacaa gccacatctg
     3601 tctgcttaag ctttctcgcc aaacgtaatt ccttgtaaaa ttcccccagt tccattctct
     3661 caatcatctg accacctcct agcttttgca tattatatca ttatttacaa tcatttgtca
     3721 aaatattctg atatttaaga gggcaaaaaa gccagcctta agctgactct aaaaggaccg
     3781 agccttgtac aatacagaac tcaatcctcg ttctaaatag aatgtctaac ttttttgggc
     3841 agtacacgag cttggggaac agccactgga acgatgaggt gtgagctcaa aatatcctcc
     3901 agttatgttt ttcctaatag tataccggaa gagtgaaagg attttataat ggagcggtta
     3961 caaagaacct actttctatt aaacagtata ctatgaaaat gtgaaaattt aacatttttt
     4021 tgtacaaatt ttataaatta ttgccttttt aatatcaata gttaatctct tatccagatc
     4081 ccccttgtgt aaactttatc tttataagct tcaaggcccc tatcccatct atttgcaaca
     4141 attagatcac tttgttttgt aaatagttca aaattctttt caataattac gttatctata
     4201 ctaacgttta aatttggttc atatactaaa atttttatac cgacaatcaa tagttcatta
     4261 attatactta aaatagctga ctctttgtaa ttatctgaat tatatttcat ccccaa
//
LOCUS       DAVHIT010000111         4296 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVHIT010000111 DAVHIT010000000
VERSION     DAVHIT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.113"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            2..730
                     /locus_tag="VVN16_001908"
     CDS             2..730
                     /locus_tag="VVN16_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5573219.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1190..1384
                     /locus_tag="VVN16_001909"
     CDS             1190..1384
                     /locus_tag="VVN16_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5573220.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(1750..3060)
                     /locus_tag="VVN16_001910"
     CDS             complement(1750..3060)
                     /locus_tag="VVN16_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5573221.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            3297..4169
                     /locus_tag="VVN16_001911"
     CDS             3297..4169
                     /locus_tag="VVN16_001911"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU5573222.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         1356 a          697 c          909 g         1334 t
ORIGIN      
        1 aatgagtaca aaatatttat ttatttacaa tgagattcgt gaaaagattc tttgtaataa
       61 atataccatg aacgaacaat tacctgatga aatgacatta gctaaacagt ttgcctgtag
      121 tcgaatgacg atcaaaaaag ctttagactt gttagtttct gagggcttaa tttttagaaa
      181 acgtgggcag ggaacctttg ttctctctcg tggcagctca aaaagaaaat taatcgttcc
      241 agaaagagat atccggggac tgacaaaaat atctgaagat gctcattcta caattgactc
      301 gaggattatt cacttcaaat tagaatttgc aaatgaattt ttagcagaaa aactacaggt
      361 cgctttgcag agtccagttt ataatattta ccgcctgcgt attattgacg gtaaacctta
      421 tgttctggaa caaacttata tgagtaccga tgttattcca ggtattactg aagatatttt
      481 acaaaaatcg atttacaatt acattgaagg aaagttagga ttgcatattg ccagtgctac
      541 aaaaatctta cgagcttctt ctagttcaga aaatgagcaa cattacttgc agctccttcc
      601 aacggaaccg gtatttgaag tagaacaagt ggcttatttg gataacggaa ctccgtttga
      661 gtactcgatt agtcgtcatc gctatgattt atttgaattt aattcttttg cattacgaca
      721 ttcctcctag gagaaaatgt gaaaatgaag ccaatctttt acagactcta gtttaagaaa
      781 aatttaaaac agggcaagaa ggtcccatct atgcttaaat ggtttctctt ttctaaataa
      841 gatggcttta aaagagtgat cgttttatcc atcatgttga aaaatatctt cgtatagctt
      901 atagagtagg tactgaaatt gttcacctga tctacttctt atagttattt agttttaaat
      961 agtgtttcaa atattcttac actgacgaga agtttttgag tcttttcttg taacacatat
     1021 agtatactga ggttagaata gtagactgtg acttctaaca aattgctaga aatgaatttc
     1081 aatctcccaa tttatttgtt caaatattgt tttaatatat tgaataaatt ctgaatcata
     1141 atcatttaaa aaaattttat ttttttattt ttcattacga ataatataga tgaaggggaa
     1201 agagtatgaa aacagaactg tttcttttgc tattagttca aaaggagaaa aaatgaaagt
     1261 agaaaatatt tcgtataggg tggatcatcg tatattgttt gataatattt cttttgatac
     1321 ttcgagttca ggcgtgacat taattactgg taaaaatggt acaggaaagt caactttact
     1381 atagtagatt gaaactagaa tagtacacat ctacttctaa aatattgtta gaaatcgatt
     1441 tgactatcct gatctatttg tcctgttctt atttcatttc actatatctc aaattgagta
     1501 tgacgaagtg cgctcccatg tcctgggaac gcactttctt catatttttc atattcttga
     1561 atccatcgat aaagactatt gggatgaatt tttaaagttg aactaatcat ttttacagga
     1621 tgagatttac agcagagagt ttgaaggctt tatcaaaggt ttttcttggc ataatgactt
     1681 ttcctcgttt ccacttaatt ttgtgtctac tttattatac caagtccacc cttaagttag
     1741 ataataaatc taacttaagg aagctagaag gatgagaatc caggtggtca agagtcccaa
     1801 acttaagctg atggggacac ccagaataat ttgctttttg aaggcaaggc cacgttcctc
     1861 tatattggga agtgagagtt gaatgagaga accagctgat gaaaagggtg agatattagt
     1921 agatagagcg ccaataacgg tggctgttgt gagtaagtga atatcaatct gaggattttg
     1981 agcactgatg atagcaatga tgggaaagag ggctggagct acaacggata gggtggaact
     2041 aaagagtgac atcactccgg ctatcacaca aaagaacaga ggtaaccaga aatgaggaat
     2101 ggttgttgtc atgaggtgcc ctatcagtgt gactaaacct gacttgaccg ctagagacat
     2161 tagtaagctc atgccgcaga gcatgataat tgtagcccag ggaaccttag ctaaaatggc
     2221 ttcttgcttc cctaatttga gccttaaggc gaggcagacc atgagtattg agacaaagcc
     2281 aatatcaaat gttttttgat aagtagctat ccaggcgatg tttgggaaaa tgagatgcaa
     2341 caagggaaaa agccaaacca aaaccatgct gctgatcatg agcaaggtgg tttgtctttg
     2401 aaccttgctg aggagtggtg gttggtcaat agtcaaggat gagtttgttc ttcccttact
     2461 atagtgactg taacaggata ataaaagcaa gacgatgagt gggtagataa tgctgacgat
     2521 aaagatatga ttgccaagtg aaaaagcttg ctcttcccat cccatttgct taaacaggcc
     2581 ttgaaagaca atgcctgagc tactggttat caaattagcc cctcctgaag ctccccaatt
     2641 gacggcttga gctccaatca aagggtgttt gtccgctttt tgacagaggg taatcgctag
     2701 aggacagcaa acggccatag tagtgaaaaa tccagcacct aaagcagaca aaagggttgc
     2761 catcaggtat aaaatcatgt agagggcgtt agggtgggtg cgtgtgcggt agagaatgtg
     2821 ttgagccaaa acatcaagag taccgttagt tgttgcaacg ttataaaaga gagagacgct
     2881 aaaaatggta aaaaagagtg aggttggcca aaaatgaaga agttctttgg ggcttaatcc
     2941 catgagagtg gttgcgatga ggtaagaaaa agcaatagcc agcaggccaa tattgatttt
     3001 ggtgcggtaa ccaattccaa tggctagagc aatggcgcta atcattatta aatgaatcat
     3061 tgaattgtcc tttcagttag aatatggaaa taggttggca attttggcgc tcaaaagacc
     3121 acctgtttcc tattggtact tgactattat acacaaaaaa aatacaattg tcttgacaat
     3181 tgtattgaca cttgttagaa ttaaaaatga ggtattcagg cttgtgattt cctatggaag
     3241 ttaatagtga ttgcctctaa tgcttacaag tgatattaaa aatagaggac ctagtgatgt
     3301 caatcatttc aactgattta accccttttc aaatagatga tacattgaaa gcagccttgc
     3361 gagaagatgt tcattccgaa gattacagta ccaatgccat ttttgatcat catggccaag
     3421 ccaaggtgtc gctttttgcc aaggaagctg gtgttttagc ggggctaacc gtttttcaaa
     3481 gggtttttac cctatttgat gccgaggtga ccttccagaa tcctcatcaa tttaaggatg
     3541 gggatcgttt gactagtggc gatttggttt tagaaatcat aggctcggtg agaagtctct
     3601 taacatgtga acgcgttgcc ttgaattttt tacaacattt atcagggatt gcttcgatga
     3661 cagctgctta tgtagaagcc ttaggcgatg attgcattaa ggtatttgat actcgaaaaa
     3721 ctactcctaa tttacgtctt tttgagaaat atgccgtgag agttggcggt ggctataatc
     3781 atcgctttaa tttatcagat gctatcctgc taaaagacaa tcacattgcg gcagtaggta
     3841 gtgttcaaaa ggcaattgct caagcgcgtg cctatgcccc ttttgtgaaa atggtcgagg
     3901 tggaagtgga aagccttgct gctgccgaag aagctgcggc ggcgggtgct gatattatta
     3961 tgttggataa tatgtcattg gaacagattg aacaggccat taccctaatt gcaggacgtt
     4021 ctcggattga atgttctgga aatattgata tgaccactat tagccgtttt cgtggtttag
     4081 cgattgatta cgtctccagt ggtagtttaa cccatagtgc taagagtctt gatttttcca
     4141 tgaaggggtt aacctacctt gatgtctaag ttgtaaaata aactaacttt ttaaaggatg
     4201 tctttcctct agaacgagtt ttatgtcaga tagtttaaac gcctcttcaa atatagtaaa
     4261 atgaatcaaa aatagtacac aatgtggtat aatctt
//
LOCUS       DAVHIT010000112         4292 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVHIT010000112 DAVHIT010000000
VERSION     DAVHIT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.114"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            15..1076
                     /locus_tag="VVN16_001912"
     CDS             15..1076
                     /locus_tag="VVN16_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5573223.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNDLKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            1093..2097
                     /locus_tag="VVN16_001913"
     CDS             1093..2097
                     /locus_tag="VVN16_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573224.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSATQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIKEKVTMYINPRDIMQF
                     "
     gene            2109..3803
                     /locus_tag="VVN16_001914"
     CDS             2109..3803
                     /locus_tag="VVN16_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5573225.1"
                     /translation="MRKSLNWKDCLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFRPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNIPHLILFNLVLVGTVIILLIAYTIQKLPFSYRMIR
                     AVFFSIDNDMEEAARSMGASSFYTMVRVIIPYILPVVLSVVVLNFNSLLSDYDLSVFL
                     YHPLFQPLGIVIKQSTDETATLNAQAMMFVYSVILMIMSSAALYLSSLFQGKRGKR"
     gene            3805..>4292
                     /locus_tag="VVN16_001915"
     CDS             3805..>4292
                     /locus_tag="VVN16_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5573226.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSLEG
                     YNR"
BASE COUNT         1417 a          620 c          805 g         1450 t
ORIGIN      
        1 aaaggagaac tatcatgttc aaaaacttta aaaagattgc attcacttct tcactaattt
       61 tacttgctgc ttgctcaaac aatgcttcaa atacaaatca gtctactgat gaaggaagtg
      121 ttgataaatc tcaagaaatt gtaatctata caaattcggc ttctaatgga cgtgcagatt
      181 ggttacaaac taaggcatca gagcaaggat acaatcttcg tttggtagat atttcaggtg
      241 gagaactagc tgaccgtttg attgcagaaa agaataatgc tgtggctgat atggtaattg
      301 gattaaataa attagaattt aaccgtatta aagctgaaaa acttcttgta aaatactcac
      361 caaaatgggc tgatgaagta gatagctctt tgggagattc tgaagggtat tattcaccaa
      421 ttgtaaatca accattagtt ttgattggta ataaagatgc taaaatgcca tccgactgga
      481 cagattttac tgattcatct tataaaggta aatatggtat ttcaaaatta agtacaggta
      541 catctaagaa catctttgca agtattgttt cgagatataa agatgaaaaa ggtgagttag
      601 gaatatctga tgaaggttgg aaagttgcaa aagcttacct taaaaatgca cacgtttatg
      661 cagaaggtga agactatatc tcttcaatta tggataataa taatgatcta aaatatagta
      721 tgatgtgggg atctggtgtt ctacaaaatc aaaaagaacg tgaatataaa ttccaagtaa
      781 tgtcaccaaa agttggtgtt ccttatgtaa ctgaacaagt tgggatttta aatacaagta
      841 agaagcaagc attattaaaa gaatttgtag attggtttgg ctcttcagaa cttcaaaaag
      901 aatggtctga taaatttggt tcaattccag ctaataaaaa agctctagag caagcaaaag
      961 atgaactcaa acagtttgct aatagtgtta aaccacaaga acttgactgg gagtttatcg
     1021 ggaaaaacat cgattcttgg attgaaaaag cacagcttga atttgttgaa caataatagt
     1081 taggattaag tgatgattac atttaaggat attcaaatta agtataatga ttttattgcc
     1141 atcgataatc tcaatttaac aatcaatgag ggagagtttt tcacattttt aggaccaagt
     1201 ggttgtggga aatcaacaac tcttcgtgca ctagttggat ttttggagcc ttcacagggg
     1261 caaatactag ttgataatca agatattacg aatttagatc cggaaaaacg cgaaattggt
     1321 attgtgtttc aaagttatgc attattccca acaatgactg tttttgataa tattgctttt
     1381 ggtctaaaag ttaaaaaagt ttctcctgaa attattaaag ctaaagttca tgaaattgcg
     1441 aaaaaaatta atatctcaga cgagcaacta aaccgaaata tttctgaatt atcaggggga
     1501 caacaacagc gtgtagcttt agcacgtgca ttagtattag aaccaaaaat tctttgtttg
     1561 gatgaaccgt tatcaaacct agatgccaaa ctacgagttt cgttgagaaa agaattgaaa
     1621 cgattgcaaa aagaactagg aatcactact ctttatgtca cacatgatca agaagaagca
     1681 ttaacattat cagataggat tgctgtgttt aataatggat ttattgagca agttggcacg
     1741 ccgtatgata tttataatca atccgcaact caatttgtct gtgattttat tggctcaatc
     1801 aataaaatta gccaagaagt tcttaaaaaa ttacaaggcg agaataaata ctttaaagaa
     1861 actgaaggat acatacgctt agagcgtgtt aagttagaga aaacttcacc tcaagattta
     1921 tctataacag gggtaattag tgatattgag tttaatggtg tacttattaa gtataaagta
     1981 gacttgtcga atagtgaaag tattcatatt atcgaaaaga atgatggact atcacgtcat
     2041 ataatcaaag aaaaagtcac tatgtacatt aatccgcgtg atataatgca attttaagga
     2101 gattgtgcat gagaaagagt ctaaactgga aagattgtct cataagatta ggagttttat
     2161 ggctaatctt tgccctgatt atttatccaa atattgaact tttgtctgga gtgttttata
     2221 aaaatggttc atttactcta gaggcattca ataagattat acactctcag agagcgatga
     2281 gtagtatatt taatagcttt attttggcga tttcaatggt ctttactgta aatattgttg
     2341 ggacattgat tgttctactc acagagtact gggatattag gggttcaaaa atacttaaat
     2401 taggttatat gagctctcta gtatatggag gaattgtatt agcgacagga tataaatttg
     2461 tttatggcgc taatggtctt gtaactaaaa cgctctcaag tataattcca ggtcttgccc
     2521 ctaactggtt tcaaggattt ggtgcagtat tgtttattat gacatttgct tgtacctcaa
     2581 atcacataat gtttttaaca aatgcagttc gtagtgttga ttatcataca attgaggcag
     2641 ctaaaaatat gggtgcaagt cctttaaaag ttttctttag tgtggttttt ccaactttta
     2701 aacctacgtt atttgcctta accatcttaa cttttttgac aggtctttca gcagtttctg
     2761 ctcctttgat agtagggggg aaagattttc aaactattaa cccattgatt atcagtttcg
     2821 ctaaaactac aagttcgaga gatttagcag ctttactagc tatcatgtta ggtttagcaa
     2881 ctattctatt attgacactt ttgaatcatg tcgaaaaagg aggtaattat gtatctgttt
     2941 cgaaaaccaa ggctagtatt aagaagcaga taatttcttc tccattatgg aatactcttg
     3001 ctcacattgt tgcgtatgta ttgtttgcaa tctatatgtt accaatagtt ttgataattc
     3061 tatactcgtt tacagactcg ttgacgatca agacgggaac tttagatcta tctaaattta
     3121 cgttagcgaa ctatgctaat ttatttacag atgctcaatc atttcgtcct tacctagtca
     3181 gcgttgttta tgcaatttta gctgcggtaa ttgctacaat tatagcaata gtaatttcac
     3241 gtattgtaca taaaaacaag agtaagttcg ataaattctt tgagtatggt gcactgatac
     3301 catggatatt gccaggtaca ttgattgcac taggattaat gttcacttat aatatacctc
     3361 atttaatact atttaattta gttttagtag gaacagtaat tattttactt attgcatata
     3421 ctattcaaaa attaccattt tcttatagaa tgattcgggc tgtatttttc agtatagata
     3481 atgatatgga agaagctgca cgtagtatgg gtgcatctag tttctacact atggtgcgag
     3541 ttattatacc gtatatttta ccagtcgtat tatcagtagt tgtgttaaac tttaattctt
     3601 tattatctga ctatgactta tctgtattct tgtaccatcc acttttccaa ccattgggaa
     3661 tcgttattaa acagtctact gatgaaacag caactttaaa tgcacaggca atgatgtttg
     3721 tttattctgt aattttaatg attatgtcta gtgcagctct ttatttatca agcctattcc
     3781 aaggtaaacg aggtaagaga taatatgaat ctttcatacg aactgagtat ttgggaaata
     3841 cttcttaggt gctttttagc gattcttttc ggtggcattg ttggctatga gcgaggagtc
     3901 aaaaaccaag cagcagggat tagaacctac ttactagttt gtttgggaac ttgtgttgta
     3961 atgatgacaa atcagttcat cactagtcgt tatggtagcg gtgatcctag tcgaatggga
     4021 gcccaagtaa ttagtggctt gggtttccta ggggcaggaa caatcatggt aactaatcaa
     4081 aaagttaaag gtttaacaac agcagctgga ttatggtctt cagcaactat tggtttagct
     4141 ataggcattg gtttttatga aggtgctatt ttagccggag ttgtactcat agcagtttta
     4201 actctattta ctcctcttaa agattattta aactcgaaaa ctaaaattgc tgattactac
     4261 ttggttttta gttctttaga aggttataat cg
//
LOCUS       DAVHIT010000113         3743 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVHIT010000113 DAVHIT010000000
VERSION     DAVHIT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.115"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1111
                     /locus_tag="VVN16_001916"
     CDS             <1..1111
                     /locus_tag="VVN16_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5573227.1"
                     /translation="FIAERFSISRRGMVYFLTLEGISLYLLNFLVKKYWKHVFFNLKN
                     SKKILLLTVTKNMEKVLDKLLESDELSWKLVAVSVLDKSDFQHDKIPVIEKEKIIEFA
                     THEVVDEVFVNLPGESYDIGEIISRFETMGIDVTVNLKAFDKNLGRNKQIHEMVGLNV
                     VTFSTNFYKTSHVIAKRIIDVMGALVGLILCGLVSIVLVPLIRKDGGSAIFAQTRIGK
                     NGRQFTFYKFRSMCVDAEAKKRELMEQNTMQGGMFKVDDDPRITKIGRFIRKTSLDEL
                     PQFYNVLKGDMSLVGTRPPTVDEYVQYTSEQKRRLSFKPGITGLWQVSGRSKITDFDD
                     VVKLDVAYIDNWTIWKDIEILLKTVKVVFMRDGAK"
     gene            1115..1858
                     /locus_tag="VVN16_001917"
     CDS             1115..1858
                     /locus_tag="VVN16_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU5573228.1"
                     /translation="MRDRIQLLGVTIDLLTMNETIDIVEQYVLEKRPLHLMGVNADKI
                     NQCHTDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEVLQDMLKVFKRDYPNLIVIGHRNGYFSEEDEQAIQEDIREKNPDFVF
                     IGITSPKKEYIIQKFMDSGVNSVFMGVGGSFDVLSGHIQRAPLWMQKSNLEWLFRVAN
                     EPKRLFKRYFVGNISFIGKVLKAKRGVKY"
     gene            1855..2679
                     /locus_tag="VVN16_001918"
     CDS             1855..2679
                     /locus_tag="VVN16_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU5573229.1"
                     /translation="MNQTEMIRLIQKVELDAIKEFKKICEENDIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREAYDKLPSVFKDRIIAGKYQVLTYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSVLRKIYFGKVYWYRFLASLGTTYVGDH
                     VDMHSTKQKLIIGFFKKLGFAKLFPQNSVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDTFFKVPTEYDRYLKRLYGENYLYEEPSDDEKKSHLGGH"
     gene            2681..3559
                     /locus_tag="VVN16_001919"
     CDS             2681..3559
                     /locus_tag="VVN16_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5573230.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNYNAKQIVIIDNFSNNGTG
                     EKLQELYESDLEIDVLINHENAGFARGNNVAYQFAKEKYNPDFMVIMNNDIEIETENF
                     EKIVTDIYREEKFHLLGPDIFSTTYQLHQNPKRLTHYTYGEVKALNEKFKKGSQVSLA
                     LKIKCWLKASKVLRTAIYQNRRKKGSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIRVLHHQNVATNQVYTNLLEKTLFSNKCNF
                     KSTSYFLKLMKENEDV"
BASE COUNT         1275 a          499 c          759 g         1210 t
ORIGIN      
        1 ttttatagcg gaacggttta gtatctctag aagaggaatg gtatacttct taactttaga
       61 aggaatatcc ttatacttgt taaatttctt agtaaagaaa tattggaagc atgtgttttt
      121 taatctaaaa aatagcaaga aaattttact gttaacagta acgaaaaata tggaaaaagt
      181 tcttgataaa ttgctagaat ctgatgaact ttcatggaaa ttggtagcag taagtgtttt
      241 ggataaatct gattttcaac atgataaaat acctgtaatt gaaaaggaaa aaattattga
      301 atttgcaacg catgaagttg tggatgaggt gtttgtcaat cttccaggag agagctacga
      361 tattggagaa attatctcta ggtttgagac aatggggata gatgtaactg taaatcttaa
      421 agcatttgat aagaatttgg gtcgcaataa acaaattcat gagatggtag gattgaatgt
      481 agtcactttc tctacaaatt tttataaaac tagtcatgta attgctaagc ggattattga
      541 tgtcatgggt gcattggtag ggctgatact atgtggttta gtcagtattg tattggttcc
      601 tttgattcga aaggatgggg gctctgctat ttttgctcag acgcgtatag gaaaaaatgg
      661 tcgtcagttc actttttata agtttcgctc tatgtgtgta gatgccgagg cgaaaaaaag
      721 agaactcatg gaacaaaata ccatgcaggg tggaatgttt aaggtggacg atgatcctcg
      781 tatcacgaaa attggtcgtt ttatacggaa gactagcttg gacgagctac cacagtttta
      841 taatgttcta aagggagata tgagtttggt tggcacacgg ccacctacag tggacgagta
      901 tgttcagtat acttcagaac agaaacgtcg actcagcttt aaacctggta ttacaggttt
      961 atggcaggtt agcggccgta gtaaaataac cgattttgac gatgttgtaa aattagatgt
     1021 ggcttatatt gataattgga caatctggaa agatattgaa attttgctta aaactgttaa
     1081 agttgtattt atgagagatg gagcgaagtg aggaatgagg gatagaatcc aacttttagg
     1141 tgtaacaatt gatttgctta cgatgaatga aacgatagat attgtagaac aatatgtatt
     1201 agaaaaaaga ccactacact tgatgggggt gaatgctgat aaaattaatc agtgtcatac
     1261 agatgagaaa atcaaaaaaa tcgttaatga gtcaggaatc attaatgcgg atggagcatc
     1321 agttgttctt gcaagtaagt ttttaggaac gcctgttcct gaacgagtag cgggtattga
     1381 tttgatgcaa tgtcttttag agttgtcaaa taaaaaagga tattcagttt acttttttgg
     1441 agcaaaagaa gaagttttgc aagatatgct caaagtattt aagagagatt atccaaattt
     1501 gatagttatt ggacacagaa atggctattt ttctgaagag gatgaacaag ctattcaaga
     1561 agatattcgt gaaaagaacc ctgattttgt gtttattgga attacgtctc ctaaaaaaga
     1621 atatattatt caaaaattta tggatagtgg cgtcaattcg gtatttatgg gagttggcgg
     1681 tagttttgat gtcttgtctg gtcatatcca acgagcacct ctatggatgc aaaagtcaaa
     1741 tttagagtgg ttattccgtg tagctaatga gcctaaacgt ctctttaaac gttattttgt
     1801 agggaatatt tcattcatag gaaaagtttt aaaagcaaaa agaggtgtaa aatattgaac
     1861 cagacagaga tgattcgctt aattcaaaaa gttgaattag atgctataaa agagtttaaa
     1921 aaaatctgtg aagagaatga tatagatttt ttcctccgcg gtggtagtgt acttggtgcg
     1981 gtcaaatacg acggctttat tccatgggat gatgatatgg atatcgctgt ccctcgtgaa
     2041 gcatacgaca aacttccaag tgttttcaaa gatagaatta tcgctgggaa atatcaggtt
     2101 cttacttatc aatactgtga tacgttgcat tgctactttc ctcgactatt ccttttagaa
     2161 gatgaaagaa aacgtttggg cttgccacga aataccaatc taggattgca tttgattgat
     2221 atcattcctt tagatggagc accaaatcat tcggttttaa gaaagattta ctttggcaaa
     2281 gtatactggt atcgtttttt agcaagttta ggaacaactt atgttggcga ccatgtggat
     2341 atgcattcca ctaagcaaaa actaattatt ggtttcttta aaaaactagg atttgcaaaa
     2401 ctatttcctc aaaattctgt atacagacgc ttggataatc tctataaaaa gtatgattgg
     2461 aaaaagcaga agtatgcggg gactatcaat gcttctttat ttgctaaaga agttatgcca
     2521 gtagagattt ggggagaagg agtagagaag ccttttgagg atactttctt taaagttcca
     2581 acggagtatg atcgctacct gaaaagactt tacggagaaa actatcttta cgaagagcct
     2641 agtgatgatg aaaagaaatc gcatttagga ggacactaat ttgttttgtt atattatttt
     2701 gcattacaaa gtcttagaag aaactatttc ttgtgttaaa tctataaaag aaggcaatta
     2761 taatgcaaag caaatcgtta ttattgataa tttttctaat aatggtactg gtgaaaaact
     2821 acaagagctt tatgaatcag atttagaaat tgatgttttg attaaccatg aaaatgctgg
     2881 ttttgctcgt ggaaataatg tggcttatca atttgctaag gaaaagtata accccgattt
     2941 catggttatc atgaataacg atattgagat agaaacagaa aattttgaaa aaattgtgac
     3001 agatatctat cgtgaggaaa aattccattt gctcgggcca gatatcttct cgactactta
     3061 ccaacttcac caaaacccaa aacggttgac acattatact tatggagaag ttaaagctct
     3121 aaatgaaaaa tttaaaaaag ggagccaagt tagtctagct ttaaaaatca aatgttggtt
     3181 gaaagctagt aaagttcttc gaacagcaat ctatcaaaat agacgtaaaa aaggatcagt
     3241 agactataga aaacaggtag aaaacccaat tcttcatggt tcttttattg tatattcgag
     3301 agattttatc gaaaaagagg agtatgcttt taaccctaac accttcttct actatgaaac
     3361 agagatatta gattatgaag ctgaattaaa aggatacaag agaatttata cacctaaaat
     3421 tagagttttg caccatcaaa atgttgcaac taatcaagtt tacacgaact tgttagaaaa
     3481 aaccttgttt tcaaataaat gcaactttaa atccaccagt tattttttga agttgatgaa
     3541 agaaaacgag gatgtttaaa tgagttattt atttttactt tgccttacat tattcttatt
     3601 gactatattc tatttctttg cttttattca agatttaatt gctcctccag tagttatgtc
     3661 tgtaatgttt ctaattagtt cagtatttgc actggttaat tcaaaaaact ggaatattga
     3721 atatagtgga atagcctata ttc
//
LOCUS       DAVHIT010000114         3724 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVHIT010000114 DAVHIT010000000
VERSION     DAVHIT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.116"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            90..1271
                     /locus_tag="VVN16_001920"
     CDS             90..1271
                     /locus_tag="VVN16_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5573231.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1329..2087
                     /locus_tag="VVN16_001921"
     CDS             1329..2087
                     /locus_tag="VVN16_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5573232.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEACAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            2300..3661
                     /gene="dnaA"
                     /locus_tag="VVN16_001922"
     CDS             2300..3661
                     /gene="dnaA"
                     /locus_tag="VVN16_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5573233.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHVKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1327 a          645 c          664 g         1088 t
ORIGIN      
        1 atccacagtt tgtgtaaaac ttttgtgttt aagttataat taagctagtc agtttatact
       61 ttcagtaatt caaacatatg gaggcaaata tgaaacatct aaaaacattt tacaaaaaat
      121 ggtttcaatt attagtcgtt atcgtcatta gcttttttag tggagccttg ggtagttttt
      181 caataactca actaactcaa aaaagtagtg taaacaactc taacaacaat agtactatta
      241 cacaaactgc ctataagaac gaaaattcaa caacacaggc tgttaacaaa gtaaaagatg
      301 ctgttgtttc tgttattact tattcggcaa acagacaaaa tagcgtattt ggcaatgatg
      361 atactgacac agattctcag cgaatctcta gtgaaggatc tggagttatt tataaaaaga
      421 atgataaaga agcttacatc gtcaccaaca atcacgttat aaatggcgcc agcaaagtag
      481 atattcgatt gtcagatggg actaaagtac ctggagaaat tgtcggagct gacactttct
      541 ctgatattgc tgtcgtcaaa atctcttcag aaaaagtgac aacagtagct gagtttggtg
      601 attctagtaa gttaactgta ggagaaactg ctattgccat cggtagcccg ttaggttctg
      661 aatatgcaaa tactgtcact caaggtatcg tatccagtct caatagaaat gtatccttaa
      721 aatcggaaga tggacaagct atttctacaa aagccatcca aactgatact gctattaacc
      781 caggtaactc tggcggccca ctgatcaata ttcaagggca ggttatcgga attacctcaa
      841 gtaaaattgc tacaaatgga ggaacatctg tagaaggtct tggtttcgca attcctgcaa
      901 atgatgctat caatattatt gaacagttag aaaaaaacgg aaaagtgacg cgtccagctt
      961 tgggaatcca gatggttaat ttatctaatg tgagtacaag cgacatcaga agactcaata
     1021 ttccaagtaa tgttacatct ggtgtaattg ttcgttcggt acaaagtaat atgcctgcca
     1081 atggtcacct tgaaaaatac gatgtaatta caaaagtaga tgacaaagag attgcttcat
     1141 caacagactt acaaagtgct ctttacaacc attctatcgg agacaccatt aagataacct
     1201 actatcgtaa cgggaaagaa gaaactacct ctatcaaact taacaagagt tcaggtgatt
     1261 tagaatctta attgacatct atgtaaagaa agctttacat aagagaaaag atgtgttagt
     1321 gtagaatcat ggaaaaattt gaaatgattt ctatcacaga tatacaaaaa aatccctatc
     1381 aaccccgaaa agaatttgat agagaaaaac tagatgaact agcacagtct atcaaagaaa
     1441 atggggtcat tcaaccgatt attgttcgtc aatctcctgt tattggttat gaaatccttg
     1501 caggagagag acgctatcgg gcttcacttt tagctggtct acggtctatc ccagctgttg
     1561 ttaaacagat ttcagaccaa gagatgatgg tccagtccat tattgaaaat ttacagagag
     1621 aaaatttaaa cccaatagaa gaagcatgcg cctatgaatc tctcgtagag aaaggattca
     1681 cccatgctga aattgcagat aaaatgggca agtctcgtcc atatatcagc aactccattc
     1741 gtttactttc cttgccagaa cagattctct cagaagtaga aaatggcaaa ctatcacaag
     1801 cccatgcgcg ttccctagtt gggttaaata aggaacaaca agactatttc tttcaacgga
     1861 ttatagaaga agatatttct gtaaggaagt tagaaactct tctgacagag aaaaaacaaa
     1921 agaaacagca aaaaactaat catttcatac aaaatgaaga aaaacagtta agaaaactac
     1981 tcggattaga tgtagaaatt aaactatcta aaaaagacag tggaaaaatc attatttctt
     2041 tttcaaatca agaagaatat agtagaatta tcaacagcct gaaataaggc tgttctttta
     2101 tttttttatc tcacaaggtt atccactatg tttttcgata aaaagcttaa taaatcaata
     2161 atttcttctt ttatccccaa cctgtggata aagtttggta acattgtgga ttatttttca
     2221 cagcttgtgg aaaattcttg ctatctatgg taaaatatct ctagtattaa acttttaaat
     2281 agtaaaggag gagaaaggat tgaaagaaaa acaattttgg aatcgtatat tagaatttgc
     2341 acaagaaaga ctgactcgat ccatgtatga tttctatgct attcaagctg aactcatcaa
     2401 ggtagaggaa aatgttgcca ctatatttct acctcgctct gaaatggaaa tggtctggga
     2461 aaaacaacta aaagatatta ttgtagtagc tggttttgaa atttatgacg ctgaaataac
     2521 tccccactat attttcacca aacctcaaga tacgactagc tcacaagttg aagaagctac
     2581 aaatttaact ctttatgact atagtccaaa gttagtatct attccttatt cagatacggg
     2641 attaaaagaa aagtatacct ttgataactt tattcaaggg gatggaaatg tttgggctgt
     2701 atcagccgct ttagctgtct ctgaagattt ggctctgacc tataaccctc tttttatcta
     2761 tggaggacca ggccttggta agactcactt attaaacgct attggaaatg aaattctaaa
     2821 aaatattcct aatgcgcgtg ttaaatatat ccctgccgaa agctttatta atgactttct
     2881 tgatcaccta agacttgggg aaatggaaaa gtttaaaaag acctatcgta gtcttgatct
     2941 tttgttaatc gatgatatac agtcactcag cggaaaaaaa gtcgcaactc aggaagaatt
     3001 tttcaatacc tttaacgccc ttcatgacaa gcaaaaacag attgtcctaa cgagtgatcg
     3061 tagtccaaaa catctagaag ggctcgagga gaggcttgtc acgcgtttta gttggggatt
     3121 gacacaaact atcacacccc ctgactttga aacacgtatt gccattttac aaagtaagac
     3181 ggaacattta ggctacaatt tccaaagtga tactctagaa tacctagctg ggcaatttga
     3241 ttcaaatgtt cgagatcttg agggagccat caacgacatc actttaattg ccagagtaaa
     3301 aaaaatcaag gatatcacta ttgatattgc tgcagaagcc attagagccc gcaaacaaga
     3361 tgttagccaa atgctcgtca tcccaattga taaaatccaa actgaagttg gtaactttta
     3421 tggtgttagt atcaaagaaa tgaagggaag tagacgcctt caaaatattg ttttggcccg
     3481 tcaagtagcc atgtatttat ctagagaact aacagataat agtcttccaa aaattgggaa
     3541 ggaatttggg ggaaaagatc ataccacagt cattcatgcc catgtcaaaa taaaatcttt
     3601 gattgatcaa gacgataatt tacgtttaga aattgaatca atcaaaaaga aaatcaaata
     3661 atttgtggat aacttttagt tttttatctt ttttatccac attttttaaa caagctaaaa
     3721 aact
//
LOCUS       DAVHIT010000115         3669 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVHIT010000115 DAVHIT010000000
VERSION     DAVHIT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.117"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            68..295
                     /locus_tag="VVN16_001923"
     CDS             68..295
                     /locus_tag="VVN16_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573234.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            583..3528
                     /gene="nanA"
                     /locus_tag="VVN16_001924"
     CDS             583..3528
                     /gene="nanA"
                     /locus_tag="VVN16_001924"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU5573235.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSEEGENITLPAEHVESVSETELSGNKQEQERKDK
                     QEEKIPRDYYARDLENVETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFK
                     PDAKAPAFYNLFSVSSATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKP
                     GQWNSVTFTVEKPTAELPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRA
                     NNTVWGSNLQIRNLTVYNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESG
                     RNGKPNKDGIKSYRIPALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWG
                     DRVTITNLRDNPKASDPSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQK
                     EEAYKKIDGKTYQILYREGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGD
                     LYKGNQLLGNIYFTTNKTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKF
                     LGVGPGTGIVLRNGPHKGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVN
                     DNRQVDGQKIHSSTMNNRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTW
                     EKDIKRYPQVKDVYVQMSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTW
                     LKHNPIQKGEFAYNSLQELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLIFPT
                     EAKVKRTREMGKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFA
                     VDKEDIGQEIIGIAKGSIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTE
                     PAVHEIAEYKGSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGL
                     FTLGKKREQ"
BASE COUNT         1240 a          618 c          842 g          969 t
ORIGIN      
        1 aaatagtagt agctatgcga aataacagat agagagaagg gattgaagct tagaaaaggg
       61 gaataatatg atatttaagg cattcaagac aaaaaagcag agaaaaagac aagttgaact
      121 acttttgaca gtttttttcg acagttttct gattgattta tttcttcact tatttgggat
      181 tgtccccttt aagctggata agattctgat tgtgagcttg attatatttc ccattatttc
      241 tacaagtatt tatgcttatg aaaagctatt tgaaaaagtg ttcgataagg attgagcagg
      301 aagtatggtg taaatagcat aggctgatgt ccatcatttg cttataaaga gatattttag
      361 tttaattgca gcggtgtcct ggtagataaa ctagattggc aggagtctga ttggagaaag
      421 gagaggggaa atttggcacc aatttgagat agtttgttta gttcattttt gtcatttaaa
      481 tgaactgtag taaaagaaag ttaataaaag acaaactaag tgcattttct ggagtaaatg
      541 tcttatttca gaaatcggga tatagatata gagaggatca gtatgaatcg gagtgttcaa
      601 gaacgtaagt gtcgttatag cattaggaaa ctatcggtag gagcggtttc tatgattgta
      661 ggagcagtgg tatttggaac gtctcctgtt ttagctcaag aaggggcaag tgagcaacct
      721 ctggcaaatg aaactcaact ttcgggggga agctcaactc taactgatac agaaaagagc
      781 cagccttctg aggaaggaga aaatataact cttcctgcag agcatgtaga gtctgtttca
      841 gagactgaac tttctggcaa taagcaagaa caagaaagga aagataagca agaagaaaaa
      901 attccaagag attactatgc acgagatttg gaaaatgtcg aaacagtgat agaaaaagaa
      961 gatgttgaaa ccaatgcttc aaatggtcag agagttgatt tatcaagtga actagataaa
     1021 ctaaagaaac ttgaaaacgc aacagttcac atggagttta agccagatgc caaggcccca
     1081 gcattctata atctcttttc tgtgtcaagt gctactaaaa aagatgagta cttcactatg
     1141 gcagtttaca ataatactgc tactctagag gggcgtggtt cggatgggaa acagttttac
     1201 aataattaca acgatgcacc cttaaaagtt aaaccaggtc agtggaattc tgtgactttc
     1261 acagttgaaa aaccgacagc agaattgcca aatggtcgag ttcgcctgta tgttaatggt
     1321 gttttatctc gaacaagtct gaaatctggc aatttcatta aagatatgcc agatgtaacg
     1381 catgtgcaaa tcggagcaac caagcgtgcc aacaatacgg tttgggggtc aaatctacag
     1441 attcggaatc tcactgtgta taatcgtgct ttaacaccag aagaggtaca aaaacgtagt
     1501 caacttttta aacgctcaga tttagaaaaa aaactacctg aaggagcggc tttaacagag
     1561 aaaacggaca tattcgaaag cgggcgtaac ggtaaaccaa ataaagatgg aatcaagagt
     1621 tatcgtattc cagcacttct caagacagat aaaggaactt tgatcgcagg tgcagatgaa
     1681 cgccgtctcc attcgagtga ctggggtgat atcggtatgg tcatcagacg tagtgaagat
     1741 aatggtaaaa cttggggtga ccgagtaacc attaccaact tacgtgacaa tccaaaagct
     1801 tctgacccat cgatcggttc accagtgaat atcgatatgg tgttggttca agatcctgaa
     1861 accaaacgaa tcttttctat ctatgacatg ttcccagaag ggaagggaat ctttggaatg
     1921 tcttcacaaa aagaagaagc ctacaaaaaa atcgatggaa aaacctatca aatcctctat
     1981 cgtgaaggag aaaagggagc ttataccatt cgagaaaatg gtactgtcta tacaccagat
     2041 ggtaaggcga cagactatcg cgttgttgta gatcctgtta aaccagccta tagcgacaag
     2101 ggtgatctat acaagggtaa ccaattacta ggcaatatct acttcacaac aaacaaaact
     2161 tctccattta gaattgccaa ggatagctat ctatggatgt cctacagtga tgacgacggg
     2221 aagacatggt cagcgcctca agatattact ccgatggtca aagccgattg gatgaaattc
     2281 ttgggtgtag gtcctggaac aggaattgta cttcggaatg ggcctcacaa gggacggatt
     2341 ttgataccgg tttatacgac taataatgta tctcacttaa atggctcgca atcttctcgt
     2401 gtcatctatt cagatgatca tggaaaaact tggcatgctg gagaagcggt caacgataac
     2461 cgtcaggtag acggtcaaaa gatccactct tctacgatga acaatagacg tgcgcaaaat
     2521 acagaatcaa cggtggtaca actaaacaat ggagatgtta aactctttat gcgtggtttg
     2581 actggagatc ttcaggttgc tacaagtaaa gacggaggag tgacttggga gaaggatatc
     2641 aaacgttatc cacaggttaa agatgtctat gttcaaatgt ctgctatcca tacgatgcat
     2701 aatggaaaag aatacattat cctcagcaat gcaggtggac cgaatcgtga aaatgggatg
     2761 gtccacttgg cacgtgtcga agaaaatggt gagttgactt ggctcaaaca caatccaatt
     2821 caaaaaggag agtttgccta taattcgctc caagaattag gaaatgggga gtatggcatc
     2881 ttgtatgaac atactgaaaa aggacaaaat gcctataccc tatcatttag aaaatttaat
     2941 tgggaatttt tgagcaaaga tctgattttt cctaccgaag cgaaagtgaa gcgaactaga
     3001 gagatgggca aaggagagat gggcaaagga gttattggct tggagttcga ctcagaagta
     3061 ttggtcaaca aggctccaac ccttcaattg gcaaatggta aaacagcgac tttcctaacc
     3121 cagtatgata gcaagacctt gttgtttgca gtagataagg aagatatcgg acaggaaatt
     3181 attggtatag ctaaaggaag catcgaaagt atgcataatc ttcctgtaaa tctagcaggt
     3241 gccagagttc ctggcggagt aaatggtagc aaagcagcgg tgcatgaagt tccagaattt
     3301 acagggggag ttaatggtac agagccagct gttcatgaaa tcgcagagta taagggatct
     3361 gattcgcttg taactcttac tacaaaagaa gattatactt acaaagctcc tcttgctcag
     3421 caggcacttc ctgaaacagg aaacaaggag agtgacctcc tagcttcact aggactaaca
     3481 gctttcttcc ttggtctgtt tacgctaggg aaaaagagag aacaataaga gaagaattct
     3541 aaacatttga ttttgtaaaa atagaaggag atagcaggtt ttcaagcctg ctatcttttt
     3601 ttgatgacat tcaggctgat acgaaatcat aagaggtctg aaactacttt cagagtagtc
     3661 tgttctata
//
LOCUS       DAVHIT010000116         3633 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVHIT010000116 DAVHIT010000000
VERSION     DAVHIT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.118"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            119..1528
                     /locus_tag="VVN16_001925"
     CDS             119..1528
                     /locus_tag="VVN16_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU5573236.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1546..2841
                     /locus_tag="VVN16_001926"
     CDS             1546..2841
                     /locus_tag="VVN16_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5573237.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2846..3154
                     /locus_tag="VVN16_001927"
     CDS             2846..3154
                     /locus_tag="VVN16_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5573238.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3151..3459
                     /locus_tag="VVN16_001928"
     CDS             3151..3459
                     /locus_tag="VVN16_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5573239.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1232 a          615 c          700 g         1086 t
ORIGIN      
        1 acaaagttca agaaatgagg caaacacaag atatgtatca acaattttta aaaatgtcta
       61 gacaaatttt aaaattatgc tatactttat gtatagaata atagaaagga ggttgagcat
      121 gaaaaaaaag ttagtatttc ctaatctgtt ttggtgggga gcagcttcta gcggacctca
      181 gacagaaggt caatatggaa aagtacatga aaatgtgatg gactactggt tcaaaacgca
      241 tccagaagat tttttcgata atgtcggacc tcttgtagcc agtaactttt ttcatactta
      301 caccgaagat ttccacttga tgaaggaaat tggagttaat tctttccgca cttccatcca
      361 atggagtcga ctcatcaaga atttagagac aggtgagcct gatccaaaag gtattgcttt
      421 ctacaatgcc atcattgaag aagctaaaaa gaaccagatg gatcttgtga tgaatttaca
      481 tcattttgat ttaccagtgg aacttcttca aaaatacggt ggttgggaaa gcaaacatgt
      541 agtggagtta ttcgtgaagt ttgccaagac tgctttcaca tgctttggag ataaggttca
      601 ttactggaca actttcaatg agccaatggt cattccagaa gcaggatact tatatgcttt
      661 ccattatcca aatctaaaag gaaagggaaa agaggccgta caagtcatct ataatctaaa
      721 ccttgctagt gcaaaagtga ttcaactata tcgctcatta gaacttgatg gaaagattgg
      781 gattatttta aacttgacac ctgcttatcc aagaagtaat tctccagaag acttagaagc
      841 aagtcgattt acagatgact tctttaacaa agtcttcttg aatccagctg ttaaaggaac
      901 tttcccagaa agattggtaa aacagctaga gagagatggc gtgttatgga gtcataccga
      961 aaaagagctt caactgatga aatcaaatac ggttgatttt cttggagtaa actactacca
     1021 tccaaaacgt gttcaagcac aagcaaatcc tgaggaatat cagacgccct ggatgccaga
     1081 ccaatacttc aaagagtatg aatggccgga gcgtcgcatg aatccatatc gtggttggga
     1141 aatttttccg aaatccattt atgatattgc tatgattgtg aaggaagaat atggtaatat
     1201 cccatggttt atcagtgaaa acggaatggg tgttgaaaac gaagcacggt ttatcgatga
     1261 aaatggagtt atcgatgacg tgtatcgtat tgaattttat gaagaacatt taagatggct
     1321 acataaagcc attgaagagg gaagtcactg ttttggatac cacgcttgga ccgcatttga
     1381 ttgctggtct tggaataatg catataagaa tcgttacgga tttatctccg ttgatttaga
     1441 aacgcaaaag agaaccatca agagctcagg aagatggtat cgcaaagtaa gtgacaataa
     1501 cggttttgaa gtagaaattg aggagtaaag agaggagata ttcatatgaa aaactcaaaa
     1561 tttatagacc aatttgccac ctttgctggt aaactaggga accaaattca tttaaaaacc
     1621 ctaagagatg cattcgtaac agtaatgcca ttatatattt tggcaggttt aatcgttctt
     1681 ttgaacaaca cggtatttaa gtggattttc caaggggata cattaacaag attccaatat
     1741 tggggaataa caattgcaaa cggtacttta agtatttcag gtatgattat tgctgtaatg
     1801 gttggttatt tcttagctaa aaacagagat ttcgaaaacc cgttagcagc atcaatgcta
     1861 tcattagttt ctttaattgt gatgatgcca aatacagttt ctgtagttcc tgacggagca
     1921 aaagatgctg taaacatttc aggtgttctt tcattcaaca acacaggtac aggcgcaatg
     1981 ttcgccgggg ttatcgtagc gattattgca acagaattat tcattgaatt atcaaatgtt
     2041 aaagctttac aaatgaacct tggtgaaaat attccaccag ctgttagtag atcatttagc
     2101 gtattacttc cagtcatgac cgtcatctcc ttatttgggg ttgtttcagc attattattc
     2161 aatataactg gaatgaactt aatctcaatc attacaatct ttattcaaga accaattcgt
     2221 catattggta caagcttaat cggggtcatt attatttact ctttaggaaa tatgttatgg
     2281 ctatttggta ttcaccaagc agttatttac agtgccatcc tagaaccatt actattaatt
     2341 aacattactg aaaacatcac tgcagcaaat aatggacaag ccattccaca catcatcaac
     2401 ctatcacaaa tacaaacatt cgctttaatg ggtggtagtg gatctacatt atgtttatta
     2461 atagcaacat tcttagtgag tcgcaatgct gtctctaaaa acgtggctaa attatctttt
     2521 ggacctggta tcttcaatat caatgaacca gtattattcg gttacccaat cgtttataac
     2581 atttcattag ctattccatt tatcacagtt ccagtccttg gtattttaat cagctactta
     2641 gcaacagtta caggattcat gagtcctgca tttatacaag ttccttggac tacaccagta
     2701 ttcttaaatg catggttagc aacagcaggg gacgtgagag cagttctagt tcaattcatc
     2761 atctttgcac ttggagttct tctatacatt ccatttatca aagttaatga caaagttgtt
     2821 gaacaagaaa tggaaggtta agattatgaa acaaatttta ttagtatgta atgcgggaat
     2881 gtcgacaagt atgcttgtta aaaaaatgca acaaagtgcg actgaacgtg ggattgaaat
     2941 ctctatccaa gcaaaatcaa tgacagaagc taaaaagaat atccatgaag cagatgtcat
     3001 tctaattggg ccacaaattc gttatgagtt acttgctgtg aaagaaattg caggaaatat
     3061 ccctgtagat accattgata tgcgggatta cggaatgatg aatggagcaa aagttctgga
     3121 acaagcactt gcatggatag gagaaataag atgacagaag aaatggaata tatctgtttt
     3181 cagctcattg ccaatagtgg agcagccaaa tcatccttta ttgaagccat ccaactagct
     3241 aaggcaggaa atttaaaaga agcgaaaatt aaagttgaag aagcggaaga ctccttagta
     3301 gaagcgcaca agattcactc taatctcata caaaaagaag ctactggaga aaaaatagga
     3361 ttctccttgc tatttatgca tgcagaagac caaatggctt caacagagat tattcagttg
     3421 ctatctaaag aattcatcga tctctatcaa aataaatagg aacactgtca ttcccatctg
     3481 tctttattaa tgacgactac attgtaggaa cctccaaaag ttggacaaaa tgattgaaag
     3541 gattggatcc tgcattacac aagatccaat ccttttcttt tactctagtt tttataatat
     3601 gttgaatcta gaatagtcca ctgtagtttc taa
//
LOCUS       DAVHIT010000117         3607 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVHIT010000117 DAVHIT010000000
VERSION     DAVHIT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.119"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            485..781
                     /locus_tag="VVN16_001929"
     CDS             485..781
                     /locus_tag="VVN16_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU5573240.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPYNW
                     GAFSNYYHGSQYHWAYVGSNERDNQKTVYAGAHSAAYAFVNTNLGERITFDAGW"
     gene            884..2899
                     /locus_tag="VVN16_001930"
     CDS             884..2899
                     /locus_tag="VVN16_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5573241.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFGALTLISQISQLRSSGIRLISGEKRWSIFLRPVGDDLKGIAVGFSLAGVLTI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNFITSTEWHDY
                     SPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSELQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEILVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            2901..3533
                     /locus_tag="VVN16_001931"
     CDS             2901..3533
                     /locus_tag="VVN16_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573242.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLESYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
BASE COUNT         1097 a          632 c          751 g         1127 t
ORIGIN      
        1 atgaagaaaa atcgtggaat tcaaaaatta gctatattag tattactagg tgtttttatg
       61 tttagtaaca caattcctta ccaacagtta ttcagaagaa tagacaattg gagattcgag
      121 tgcaatcaca aaagaagtcc aatggtcttg atgttgggaa ggctgattga tttcgtatta
      181 ctttaaaatt caactattta gagaaaggta ttctgatgag agcttgtaac aataccagat
      241 agatataaga atggagatgt ttgataaaat gctttcatag ctaaccaact gttgaaaatt
      301 atgagttaca aaatctaaag tatttaagat tggtttataa aattaggtct ctgcctgtag
      361 aaaggaacgt gcagttagaa aggaggaaaa aataggatac tataattcag actgagattt
      421 ctaattaaaa tatgtagttg catttcgctc attttcataa tatttcattt agaggagtaa
      481 taatatgtta aaatttagaa aagtagttgc atcatccctt gccgttttgg catttagttt
      541 ggctcttcct gttttggcag tttctcaccg tggtggcgag tggacctatg gaggccatca
      601 tgatccatat aattggggag cattttcgaa ttattatcat ggttcacagt atcactgggc
      661 ctatgttgga agtaatgaac gtgacaatca aaaaacagtt tatgcaggag cacatagtgc
      721 ggcctatgcg tttgttaata caaatttggg ggagcgaatt acatttgatg cgggatggta
      781 aagaataatt gaagaagatt cttttatcaa tagtttagta agcttgttag tttatagagg
      841 gcaggggtat ttctgccctc tgttttagaa taaggaatgc actatgaaaa aattatttat
      901 actcatatcc aacctgcttg ctagtttgtt ctttgtttgg gtatttacca tttggactga
      961 tacatatgtc tcccattatt accccaacgt tgttgtacat gattcttccc ctgaaacaac
     1021 ttttcagcat gttgctacac gcttggaaaa actagcagaa gaaacagata gctttattgc
     1081 gattcaacac caagatccta actcagaagg tactacagtt ttttcttata cgacctttgg
     1141 agacggaaag ttgcctgacg ggctacagga aaaaaaacta gaagatgctc aaagtagtag
     1201 tgttgaaaca aactattttg tattcgatgg acacctagat attcacttgc taagggagga
     1261 gctaagtcaa cttggcttga ctaatatgca cctgacaatt ccatctaaac tatctacgtt
     1321 aatggctatc tttagtaacg gatttcagtt aatcagtcta ttgattttca tcttaacctt
     1381 tggagcactg actttaatta gtcaaattag ccaattacgg tcgtcgggca ttcgcctcat
     1441 ttcaggagag aaacggtggt ccatttttct tagaccagtt ggtgatgatt tgaaagggat
     1501 agctgttggt ttcagtttag ctggcgtgct caccattctt atgcaaaaaa ttctgtcgct
     1561 tccaacgcaa tccctaatga ccataggagc aggcttgctc agttataatc tcatcttgtt
     1621 gtctatctcc ctgtttttcg ctcaactctt cgcagttggg ataaaaaaga tacacctgat
     1681 gcagattata aaagggcaag tgcctgtcag aggaatcatt agtttgattt taatcggtca
     1741 actattagcg attattattg taacgctggg aatagggagt agcttaaagt attcccaagc
     1801 ctggcaacag catcggattg gacaagaggc ttggagccag gaaaggcaac tgattaccct
     1861 atcaatcagc cgtgaaggaa cgagtcctgg ttttgatgaa caagctcaaa ggaaactcag
     1921 aacttggtat caattgatgg atctggctgt ttcagaacaa aaggctttct tatctagaca
     1981 ccagttaatt gaccgtactt tgcaaaatgg catggcttcc tccaaaaact ttataacctc
     2041 tacagaatgg cacgattaca gcccgaatgg caatgtcctt atcgtcacac cgcaatactt
     2101 ggagcgtcaa aacattcctg tagatacaac tattgagcaa aagatgaatc accttgatgt
     2161 aggggagttt gtcttattgc tgcctgaaca cctccgttca gaggaagaac attataaatc
     2221 tgtttttgaa gacgacttaa ccagtcgcat gtctagtcaa gatgaacgac agcaaatgac
     2281 tgctacggta ggttatttag aatcaggtca ggatcgattt gtgtataata cgacccctat
     2341 ttcttaccaa caatttttga aagatccaat catcattgtt ataacacccc aatcaactgg
     2401 tccacagtcc attttgtttt ggatagacgc agtacagaac tacgttctct ttaatcaatt
     2461 gtctgatgcc caggagctta tccagagaca aggcattgaa aattgggtct cagaattgca
     2521 aacaggttac cacaactaca tcacattatt ggataatatc cagagggaac gttgggtaat
     2581 gctagcagga gctgtgcttg ggattgcaac ttcaatcttg ttgtttaaca ctatgaatag
     2641 gctctacttt gaagaattta gacgtgccat ttttatcaaa cgcattgcag gtctcaggtt
     2701 cttagaaatc catcgcactt atctctttgc tcaactgggt gtgtttttac tgggatttgt
     2761 tgcgagtgta tttcttcagg tagagatact agttgctttc ttagtcttgt tactctttac
     2821 tggtctatct cttttacagt tacatgtcca aatgcagaaa gaaaacaaga tgtccatgct
     2881 tgttttgaag ggaggttaat atgattgaac ttaaacaggt gagtaaatct tttggagaac
     2941 gagagttatt ttcgaatctt tcaatgacat ttgaggctgg aaaagtctat gccttaattg
     3001 gttcaagtgg tagcggaaaa acaaccttga tgaacatgat tggaaaatta gaatcttatg
     3061 atgggacgat tttttaccga ggtaaagact tggccaatta taaatcgagt gattttttcc
     3121 gtcacgaatt gggctacctc ttccagaact ttggcttaat tgaaaaccaa agtattgaag
     3181 aaaaccttaa gctaggtctc attggtcaaa agttgagtcg gtcggaacag cggttgaggc
     3241 agaagcaggc tttagaacag gtcggcctgg cttatcttga cctagataag cgcatctttg
     3301 agttatcggg cggagaatcg caacgggttg ccttggcaaa aattatctta aagaatccac
     3361 cctttattct ggcagatgag ccaacagctt caatagaccc agcaacctct cagttgatta
     3421 tggagatttt gctatctctt cgagatgata ataggctaat cattatcgca acacataatc
     3481 cggcaatttg ggagatggct gatgaagtgt tcacgatgga tcatctgaaa taaaaatcct
     3541 tgtttttaat tgtacgatga gttactgaaa tattatcatg aatcaataat tggagttaat
     3601 ttagaat
//
LOCUS       DAVHIT010000118         3569 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVHIT010000118 DAVHIT010000000
VERSION     DAVHIT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.120"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(50..3439)
                     /locus_tag="VVN16_001932"
     CDS             complement(50..3439)
                     /locus_tag="VVN16_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEU5573243.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENGQAAIAEEKPKLEIPSQPAPSTAPAEESKALPQN
                     PAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
BASE COUNT          851 a          696 c          787 g         1235 t
ORIGIN      
        1 aaataaacag cgagcttcaa tctcgctgtt tattttttat cgaaaagact tagtcttctt
       61 ttcttttagt gagtccatag gctgctagtg tggccatgag tcctgcgact actagtcctg
      121 cagaatcgtg agttcctgtt tcaggaagtt ttttctctat taccacagga gctggatttt
      181 gaggaagagc tttgctttcc tcagcaggag cagttgatgg agctggttgg cttgggattt
      241 ctagttttgg tttttcttca gcaatagcgg cttgtccgtt ttcatccagg cttcctgaat
      301 gagttgcagc tggtttgagg ccttgaagag cggcttttag gttggctaca agtgtgtcaa
      361 gctcagcttg tttattacgg ttgaggttgt aatttagagc tgttttagct gcgtcaaggg
      421 cctcaagact ttctttacta tatccttcta agtttgtagg aattttagct aattcttcgc
      481 ggagagcatt ataattagca cggaagtagt ctttgttgtg gtctgcaaag gcagtcatga
      541 gttcaaagat ttcctcttcc ttgtactcag cgcttggtct atctgcccag attgaaagca
      601 tacttccgac tgttggaaga tctacttcag gatatttggt agaagctagt tgattgaatg
      661 gaactttacc agaattttca actgcttttg aaagagggta agcttcatct tgtttgtgat
      721 tgcctataac ataataccag tcaccgttgg tattcaagaa tttatagcct ttgcttgcta
      781 ggtattgagg tgatgcgagg ttatatcccc accagccttt agaccagtaa gaaatcaaga
      841 catctttgtc aaactgaaca tcgtccttgt cttcatagta gaagccatcg ttgaaggcca
      901 ttggttgaag ccctctttct ttggccatag ctgcgagggt gttggcatat tcggcaaatt
      961 tgccatagag ttgataccac ttgaggtagt accagccttg ggcactagtc gcatcgttgg
     1021 cgtattcgtc agtaccaaag ttgaaaatct ttgttttacc tgcaaagaag tccatgtatt
     1081 taccgatgag ggcttttaca aagttcatcg cttcttcgtt tttcaagtcc atagttgttt
     1141 ttgaaacttt atcaaagtgg gcttgaggat ttttaatacc taatttttcc atggcaacca
     1201 gcatagcatc catgtgacct ggactgttaa tagctgggat gagaccaatg tccttagatt
     1261 tagcgtattc aattagctct gtcacttctg cctgtgttag tgcagtaccg tttggatcgt
     1321 cgtagtaagc tttagttcct tcgataatag cttttttaac gtcatcacta gcataggttt
     1381 ttccgttggc agtaatggtc atatcatcga gtagaaagcg aagtccgtca tttcctagaa
     1441 ggagatggac atcagaatat ccgagctcac tggccttgtc tacgatgcgt ttgagctggt
     1501 tcagagtaaa gtatttgcgt ccagcatcga ttgagattac cttgtttttg gcaagttttt
     1561 caacctcacg tttagcttct tcttcttttt gagcttcagg cgtgagggtc aagttgttga
     1621 cagtttcttg aagtttagca atggcttgat caatcgtatc ttgttgggca cggctaaggt
     1681 tgctatcgag agagcgaata gctttttcag cttcttttac ggccgtgacg ctttctgcag
     1741 tataacggtt caggtctttt ggtacctcgt taagtgcttg ctctgcagat tcataatcag
     1801 ctgcgaagta ttcagcgttg gcatttgcaa aatgacgcat gagtttgaag aggcgtgatg
     1861 gtgaataacg tgcagatgga gtgtcagccc aagcagctac cataccaccg atgattggga
     1921 tatcagctcc ttctgttttt ggtacagaag tgattggtgt gtttttaata ccattgagcc
     1981 cctgatcgag attgtaccag ccttggccat cagcgtttcg tccaagaacg tagtaccaag
     2041 catcattggt attaaggatt tggtgacctt tttcagctag tagtttagaa gaagcgacat
     2101 cgtagcctcc ccaaccacca gtccacatag aaacgatgat gtctttgtca aaactaccaa
     2161 agcttgtgtc gctattgtag tagataccgt cgttaaaggc cattggtttg agaccgtgcg
     2221 attttacaat acgagcgagg tcattggcgt aggcaataaa tttttcatag ccttttacag
     2281 ggtagccttc gtttggatag tatttatcag cttgaagcac actccaacct ttagcatctg
     2341 tcgcatcatt ggcatattca tcaagtccga tgttgaagat ttcagtcttt ttcgcgaaat
     2401 aagcagcata tttgtcgata agggcttttg taaaagcgac agcttgttcg ttgtcaagat
     2461 cgacagtacg ggctgatttc ttcccaaaat agctaaagtt agggttttgg attcccaatt
     2521 ctttcatggc attgagaatc gcatccatgt gtccaggact atttactgtc ggaatgagac
     2581 cgataccttt atctttggca tagttaatca gatctgtcat ttgactttct gttaagtgat
     2641 tgccgtttgg atcgttgtaa taatcatttg tacctttttc aatggcgcgt ttgacatcgt
     2701 cactggcata ggtcttgccg ttagctgtga tgctcatatc gtccaacatg aaacggagtc
     2761 catcatttcc gactaatagg tgtaaatcag tgtagccata atgtttcgct ttatcgatga
     2821 tttccttgag ctgttctggt gagaaatatt tacgtccagc atcaatagaa acaattttct
     2881 ttttcgctag tttttcattt acagttgcag cacgttcctt tcctgcctct gtttccggtt
     2941 tgtcagcctc tgctttcgct tcatcttttt tagctggttt atccttgtca gtcttgtctg
     3001 tatttgactc tttagaatca acctctttcg cttcttcctt tttagggcta gcttcttctg
     3061 cctttttatt agcagtttct ttttcagcag aagttggagt taccacttct gctttatcac
     3121 taggagttga actaacttcc tcttgtggtt tttcttctgt ttttggaaga ctagctacct
     3181 tatcagtagc tggagtttct gtttctacag tttttggagc ttctggttga agctctgctt
     3241 taggtgtttc ctcagtccga ttttcggatg attgagggga atcagaaacc gtatggatgg
     3301 tcggttggtt ttctgtagta gtaggagtaa ctccatcggc tgcaacagtc tgtgcttgga
     3361 aggcaaatcc aattagaaca gaagctgctc ctacagcgta tttacgaata gaaaaacgct
     3421 gttgtttttc atgtttcatt gcaaaacctc ctgattgcat tgttatattg atagcgatta
     3481 tataaatcaa cgcctttatt ttatttctta tattaacgag agtcaagagg agatgacaaa
     3541 aaactataat aagtataaaa aaatataaa
//
LOCUS       DAVHIT010000119         3479 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVHIT010000119 DAVHIT010000000
VERSION     DAVHIT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.121"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            172..420
                     /locus_tag="VVN16_001933"
     CDS             172..420
                     /locus_tag="VVN16_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU5573244.1"
                     /translation="MNTKMMSQFSVMDNEMLASIEGGTDWGTVGKGAVYGAGIGVAMC
                     AVGGLLTGGSTWAMTAGCAWAGAKLGGSFTAIADNLWP"
     gene            436..645
                     /locus_tag="VVN16_001934"
     CDS             436..645
                     /locus_tag="VVN16_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU5573245.1"
                     /translation="MFNYKIVDNQELSNISGGGLGGDVVVGALSGAFQAGQSCIAGGP
                     QAYLICATGGAIVGGILAYGLRPPK"
     gene            1723..1827
                     /locus_tag="VVN16_001935"
                     /pseudo
     CDS             1723..1827
                     /locus_tag="VVN16_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037603748.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1847..2422
                     /locus_tag="VVN16_001936"
     CDS             1847..2422
                     /locus_tag="VVN16_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5573246.1"
                     /translation="MFVGFLVYVIVATVGYTINQGDYFQNEHLFIIAKTFLISLSVAY
                     AKWFDMISLRRLTKKEVLLFIASFLLCVLVNIGYHSLFTVSSGAGYQHLEAASTGISL
                     SFIASATVFGPILEEFVFRGILQGVVFENSWLGLVLTASLFSFLHAPYDFPSFIYYLF
                     GGFMLGFAYKKSQKLSVAILVYICYNCLSFL"
     gene            2649..2837
                     /locus_tag="VVN16_001937"
     CDS             2649..2837
                     /locus_tag="VVN16_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573247.1"
                     /translation="MAALNTSLTTNIKFGLVGTVGGRFAGYGMYVLGYPGDYLDITNH
                     RVVSAKTSHKSYQILEND"
     gene            2867..3271
                     /locus_tag="VVN16_001938"
     CDS             2867..3271
                     /locus_tag="VVN16_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU5573248.1"
                     /translation="MKKIFATKRNVFLVRLLLGQIPLVVSTYLFLSRQFLNFSLVFQF
                     LLVIINLASILATVYLTREMRIRKFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     AESYQQIIAYIGAVLCLLIMLLLIWGLKYYKK"
BASE COUNT         1080 a          500 c          739 g         1160 t
ORIGIN      
        1 aagtgattag aaattatttt tttttaaaca tttagaggtg gtttcaaatg aaaagctaat
       61 tcaagacgtt tcgatgacaa ttcaagattt ggatgaaaaa tttaaaaaaa aaatgatata
      121 ctaaacttgt caaagttgca acaagacaaa aataaaaaag gagtatttgt catgaataca
      181 aaaatgatgt cacaattttc tgttatggat aatgaaatgc tggctagcat tgagggagga
      241 actgattggg gaacagtagg aaaaggagct gtctatggag caggtatcgg agttgctatg
      301 tgtgcggtag gaggactcct tactggtggt tccacatggg ctatgactgc tggctgtgct
      361 tgggctggag ctaaacttgg tggttctttt actgcaattg ctgataatct ttggccatag
      421 aagggaatag aaattatgtt taattacaaa atagtagata atcaagaatt aagtaatatt
      481 tctggaggtg gattgggagg agatgtagtt gtaggagcct tgtcgggtgc atttcaggca
      541 ggacaatctt gtattgctgg aggccctcaa gcttacttga tttgcgcaac agggggcgct
      601 atagtaggtg gcattttagc ttatggattg agaccaccaa aatagtttta aaaaggaatg
      661 agtgttaata tgttaaatga aactatgatt cagcttgtac tcattgcaat ttgttgtgtg
      721 tacattattt ttaacaccaa ggctgataaa aaccaaaaga gaggatatcg tacagctttg
      781 taccttttcg ttatggctgg tattatatcc tatattatga attatctgaa ctggttagat
      841 tttttcttac tgataactcc gataatgtgc ctatttaaat ttgaagataa atggagttag
      901 caacgtttga cacttcaata ggttattagc tatcctttgg ctcattgtca actgtagtga
      961 gttgaagaaa atctaagatc gagaaaggac gaatttcgtc ctttcttttt tgatgtacga
     1021 cgggcatgtc gtatatctga ggtgtaagtc ctcggtgggc acctgctacc ggtgaaccca
     1081 atagcgattc ccaagcctga ctatcgtgag gtagcaggga gaggaaggga tagcgaaatc
     1141 gtggctctac gaacaggaac gtgatagtaa ggcgtatata gcggataagg gggcctcaaa
     1201 ctctaaagtc caaaaaggta gtcgtaacct atatgcgtaa atcacgagag taaacgagga
     1261 aagatgtacg acttatcccg tgaggtctca tgagcgctaa aagcgtagta acaacgaatc
     1321 atgagaagtc agccgaaccc atagtagtga ggaaacttct gtagtggaag tggagcgaag
     1381 gggtgaacaa ttgaaagtag aattgtctgt tttgattatt tgtttgatag gatggtagta
     1441 tgaaaatata aacggtgact attgttcata aaaaaaagga cacacgaata gaacgcttat
     1501 gtagcctgag agaggagtta gttaagaaat ctttgtaaca aataaatatg attgtgttaa
     1561 tcattatttt gaaagcaaat gatatttcta ttctgcagag cgaaaaatat cataattggt
     1621 acttttacaa ctaaatatat tcgctctgtt gtaaaatgaa gtgcaacaca aaagacatgt
     1681 tcatctgata tactagagtt gcgaaaaaca gtataaagga agatgaacat gtccacaaac
     1741 tattctacca caaatcaata atacaatcac ttatctgaag ctgaaagagg agaaattgaa
     1801 gcttatttaa gcgtaggact caaacctgtt taataaatat catgccgtgt ttgttggatt
     1861 tttagtctat gtaatagtcg ctacagtagg ctataccatc aatcaaggtg attattttca
     1921 aaacgagcac ctatttatta ttgcaaagac tttcttgatt tcactgagtg ttgcctatgc
     1981 gaaatggttt gatatgattt cgctaagaag gttaacgaaa aaagaagttc tactttttat
     2041 tgcaagcttt cttctttgcg tgctggtaaa tataggttat catagtcttt tcacagtttc
     2101 ttctggtgca ggatatcaac accttgaggc tgctagtaca ggaatttcct tatcctttat
     2161 tgcaagcgcg acagtttttg gccctattct ggaggaattt gttttcagag gaatccttca
     2221 aggtgtggtt tttgaaaatt cttggttggg gcttgtactg actgcctctc tcttctcttt
     2281 cctgcatgct ccttatgatt ttccttcgtt tatctattat ctatttggag gtttcatgct
     2341 gggctttgct tataaaaaga gtcaaaaact atcggtggct attttagttt atatctgtta
     2401 taactgttta tcattcctgt agtatatgta agtaatcaat atgacataat attggacaaa
     2461 aaatcttaaa aaatagggaa atggatacta ttactgttta gattaacaac attaaatgaa
     2521 atgatgtcac aatttgagat tatggatacc gaaatttttg cgaaagttga aggaggttta
     2581 ggaggttggg aagaggtagc ttttcaaaaa attatatggt ttggttggat aatcatgtgc
     2641 gcattcttat ggcggctctt aatacttctc tcacaactaa tatcaagttt ggtctggttg
     2701 gaacagttgg aggaagattc gctggttatg gtatgtatgt tcttggttac ccaggtgatt
     2761 atctagatat caccaatcat agagttgttt ctgctaaaac atctcacaaa agttatcaaa
     2821 ttttagaaaa tgattagata tcatttaatt gtaatggagt ttaggtatga aaaaaatatt
     2881 tgcaacaaaa cgcaatgttt tcttagtgag gttgttgtta ggacagatac ctttggttgt
     2941 ttctacttat ctatttctat ctcgtcagtt tttaaatttt tccttggttt tccaatttct
     3001 tttagtgatt attaacttgg cttctatttt ggctactgtt tatctcacta gggaaatgag
     3061 gataagaaag tttgaagatg atgatttggt tagtcctaga accaatcaac tcatgtttat
     3121 cggcttgaca ggttttatgt ctattatatg tttgtataga gggatcacag cagcagaatc
     3181 ctatcaacaa ataatcgctt atattggcgc ggttctctgc ttgcttatca tgcttttgct
     3241 catttggggt ttgaagtatt ataaaaagta gagcttaatg aagtataaaa gaaaagggta
     3301 agtgttttcg caatacttac ccttttataa tagattccct gcgaaacaaa atatggtata
     3361 gtatagtata ttgaaactag aatagtacgc ctctacttct aaaacattgt tagaaatcga
     3421 tttgactgtc ctgatcgatt tatcctgttc ttatttcatt ttactatagt tctatgaat
//
LOCUS       DAVHIT010000120         3418 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVHIT010000120 DAVHIT010000000
VERSION     DAVHIT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.122"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..540
                     /locus_tag="VVN16_001939"
                     /pseudo
     CDS             1..540
                     /locus_tag="VVN16_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            644..1185
                     /locus_tag="VVN16_001940"
                     /pseudo
     CDS             644..1185
                     /locus_tag="VVN16_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1262..1483)
                     /locus_tag="VVN16_001941"
     CDS             complement(1262..1483)
                     /locus_tag="VVN16_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573249.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1448..2077
                     /locus_tag="VVN16_001942"
     CDS             1448..2077
                     /locus_tag="VVN16_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573250.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKASASEETSPAKNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2113..3336)
                     /gene="pepT"
                     /locus_tag="VVN16_001943"
     CDS             complement(2113..3336)
                     /gene="pepT"
                     /locus_tag="VVN16_001943"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU5573251.1"
                     /translation="MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEET
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
BASE COUNT         1141 a          652 c          658 g          967 t
ORIGIN      
        1 atagcatctg gatctgttgt tatcctttcc ttaagtctgt gtgtttatgc tctgaaccaa
       61 catagctaac aggccaatac agataaaaat cgtgtttcat atgtaaacag taataaagac
      121 actaagaaga ctgaaaattt gactccagac taggttagtc aaaaagaagg aattcaagca
      181 aaacaaattg ttattaagat tacagatcaa ggttatgtaa cttctcatgg cgatcattat
      241 cactactata atggtaaagt tccttttgat gcgctcttta gtgaagagct attaatgaaa
      301 gcccccaact atcagccgaa agatcaagat atcgtcagtt aggttaaggg aggatatatt
      361 attaaggtag atggaaagta ttatgtttac cttaaagatc aagctcatgc agaaaatgta
      421 cgaacaaaag atgaaatcaa tcgccaaaaa caagaacatg gtaaagacga taaaggagct
      481 agtgcagaag tatctgtagc aaaattacag ggacgttata ctaccgatga tggttatata
      541 tagtgagttg aatttagaat agcacatcat agtttctaaa tcattgttag aaattacttt
      601 aaaatcccct tatcaatttg ctcatattct atttcaacct actatagtac cacatggtgg
      661 tcactatcat ttcattcctg agagtgactt gtcggcaggt gaattagcag ccacatatgt
      721 ctttaaccct aatgatattg tcagagatat aggtgatgct tatatagtaa gacatgggga
      781 tcactatcac tatattccta agtcatcttt taacaatccc ccatcacact ctaacacgga
      841 agaggtagga agttcttcta gttctgtatt aagtaatccg tcactacatg tccaccatga
      901 ggaagaagat ggtcatggtt ttgatgccaa tcgaattata agtgaagatt cagaaggttt
      961 tgtgattcct catggcgacc acaatcacta catcaaggtt caaactaagg gatatgaagc
     1021 ggctttgaaa aacaagattc catccctgca atccaactat caacctggaa cttttgatga
     1081 gaaggcagtc ttggcaaaag tagatcaact tctagctgat agcagaagca tctacaaaga
     1141 cagattgagt tagctttaga tcagtttact gaaagtttga aaaaaataaa agttagttga
     1201 ttgattactt ctctaaattt tgggcacact aaaaagaggt cttgacacct aaactttaaa
     1261 attaaagcat attgaatccg tgctgctgtg cgaagcgaat agcgtcttgg acgaacattg
     1321 tcttatctgg atttcttatt tctgtaaggc cttgtaatct tggattataa ctctcctgtt
     1381 ttaggagtac atggtagata gcaaccaata atctgcgaca gatagcaagg aagaagtaga
     1441 aagtcttttg aacaaggcaa atcaactctt ggaacaaatc catgaagaag gaatcagaca
     1501 atccttggca gaagaagtag aaaatctaaa agctgccaca aacaaggctg atgcagactt
     1561 ggatgaagta aacagtcagg taaaagatgt tttgactcgt atcgctagcg cccttcaaca
     1621 agaaaaggaa aatgctgagc aagattctca gacacttgta ctctatcaaa aactctacga
     1681 tattctcatg tcgcttcact cttatttaga aagtaataat ggatctgatg cggactttga
     1741 taaggtggat gcattattag atcaactttc tgctaatagt aaagacaaaa cagctttact
     1801 tgaatcgaca aaagctattc tagttttgaa tcatcaaatc cagtcaaaag ctagcgcaag
     1861 tgaagaaaca agtccagcaa aaaatgctga agcaaatggt gataacacca gtgctgagaa
     1921 ccaaccaaac gcaactgcag aatctaacat tgaaactgct agcgatgaaa acaaaccaag
     1981 caatacaaga gattctaaac ctgctgaatc aacttcagaa aatgaaacaa cagaatcttc
     2041 tacaactact ggaaatcaag aaaaaccagt agaataaaaa gaaaggcgaa gtagctgagc
     2101 cacctcgtct ttttagcctt tataagctac gatgccaatg atggtatcaa ctgcacgttc
     2161 catagtctga aggctaacgt attcaaaacg tccgtgcata ttttcgccac ctgcaaagat
     2221 attcggagtt gggattccca taaaggaaat cttagagccg tctgttccac cccggattgg
     2281 ttcgataata ggcgtgatac ctagatcttc cataacggct ttagcaatgg taattggagt
     2341 catatctttt tcaatgactt ctttcatatt gtagtactgg tctgtcaagt tgagagtgac
     2401 acggtcgctc ccaagttctt cattcatctt atcagcgata gattgcatgg atgctttacg
     2461 cgcttcaaag gcatcttttt caaaatcacg aatgatgtag cttgcacgcg tctcctcaac
     2521 actacctgtc acatccatta gatggtaaaa accttggtaa ccttcagtta actcaggtcg
     2581 gtcattttct ggaagttgat tatgaaaatc aattgctagc tgaagggcat tgaccatctg
     2641 ccctttggca gtaccagggt ggacattacg gccttggaaa tgcaattcag caccagcggc
     2701 tgagaaagtc tcgtactgaa gttcacctag tggaccaccg tcaacagtgt aggcaaaatc
     2761 cacatcaaaa tcttctgcat caaatttatt ggcaccaaca ccgatttctt catctggacc
     2821 aaaaccaaca cgaatctcac agtgcttaat ttcaggatga gcagttagat attcaatggc
     2881 tgtcataatt tcagcaattc ctgacttgtc atcagcacct agcaaggttg ttccatctgt
     2941 tgtgatgagc gtttgtcctg gatatttttc aagactcttg aagtcagctg gatcgagttt
     3001 gaaaccagaa ttccctagtt caatcacacc accatcgtag ttttcaatta cctgtggatt
     3061 gactccttca gcattaaaat cagcagtatc catgtgcgat ataaaaccaa tcttacgtgt
     3121 taaagacgga tcgttggctg gcaaggttcc aatagcaaaa ccattcggta gatagtaaac
     3181 attttgcagt ccaacacgtt tcatttcagg aattaggaca tttgttgcga agtcaacctg
     3241 actctgtgta cttggagtag tagtagagtg ttcatcagag cgcgtgttga ccttaacgta
     3301 ggttaagaag tggtccaaga gatttggata agtcataaaa aaactccttt tttatcataa
     3361 aatagaatac aaaacttcgg aaaaatatga tttgagaaat tttatgaatt ttgaaaaa
//
LOCUS       DAVHIT010000121         3314 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVHIT010000121 DAVHIT010000000
VERSION     DAVHIT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.123"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(223..834)
                     /gene="rpsD"
                     /locus_tag="VVN16_001944"
     CDS             complement(223..834)
                     /gene="rpsD"
                     /locus_tag="VVN16_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5573252.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1029..2081)
                     /locus_tag="VVN16_001945"
     CDS             complement(1029..2081)
                     /locus_tag="VVN16_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU5573253.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2078..2776)
                     /locus_tag="VVN16_001946"
     CDS             complement(2078..2776)
                     /locus_tag="VVN16_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5573254.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2943..>3314)
                     /locus_tag="VVN16_001947"
     CDS             complement(2943..>3314)
                     /locus_tag="VVN16_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5573255.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT          929 a          741 c          634 g         1010 t
ORIGIN      
        1 agagaacttt tttacgaaaa tctattgaat atgccataaa aagattatac cacattgtgt
       61 actatttttg gttcacttta ctatatcact ttggactata tcggtctagt tatgtctaaa
      121 cgattatagc tggatactaa gagaaatcaa aaaagcacta agggagaacg tcccaaagtg
      181 cttaatatca aggcctcaaa gcctatctta ttcaataaaa tattacaaca ttttgttgta
      241 gaattcaacg acaagtgctt cgttgatttc tgggttgatt tcgtcgcgtt ctggcaagcg
      301 agtcaatgaa ccttccaatt tttcagcgtc gaatgatacg aatgctggac gtccaagagt
      361 agcttctact gcttcaagga ttgctggaac tttcaatgat ttttcacgaa ctgagatcac
      421 ttgacctgga gttacgcggt atgatgggat atcaacgcgt ttcccgtcaa caaggatgtg
      481 accgtggttt acgaattgac gagcttgacg acgagtagtc gcgagaccaa gacggtaaac
      541 aacgttatcc aaacgacgtt ccaaaagaag cataaagttg aaacctagga ttccaccttt
      601 gatttttgta gcttgtacga acaagttacg gaattgtttt tcacctacac cgtaagtgaa
      661 acgaagtttt tgtttttcag ccaattgcaa accgtattct gacaatttag aacggttgtt
      721 tggtccgtgt tgtcctggta cgtagttacg acgtgccaat tctttacctg tacctgtaag
      781 tgaaaggcca aggcgacgag cttgtttcca agatggtcct gtataacgtg acatatgaat
      841 gtcctcctga tataaataat atttcggtgg aaatagtcac ttagaaagcc ctgattcgtg
      901 cagatgccct tcgcctaaac agccaaggtt acttgtcata agacacctgt tgacgagctt
      961 catgctttcc tgctgatatt tcacacgaaa tccattttac catgaatagc tggatttgta
     1021 aaggggtttt aggctttatt ttcactacca gagaggttga gaacgagggt aaaggtactt
     1081 cctagaccgt actggctgct gactgtgatt tccccaccca attgatgggc caattcacgc
     1141 gcaatcgcaa gtcctaatcc atgaccacct gtcttcatgt tacgcgaagt ttcgacacga
     1201 taaaggcgtt tgaaaatatt ttccaaatcc tctggggcaa taccctgccc ttcatcggtc
     1261 acactgattg aaagctggtc cttctccagc ttagccacca cttccagctt ggttcctgga
     1321 gcagaatatt taaaagcgtt atcgaccaga ttcaccaaga tacgagaaag cttagcataa
     1381 tctccctcaa tccgggcaga ctctgggatt acctgcaagt ggacatctct tctctcctgc
     1441 tcaatcaaaa actgaaattc actcatgcac tcaattaaga gcttgtccag aaaaatactg
     1501 tctttactgg tagtttccac ctgatttcta gctgtgttta gggtcaaaaa attcaactcc
     1561 tcaaccagtt tattgagcct ctccgtctgg cgtccaatgg ttgctagata atgagcttgc
     1621 tccgactcct tgataatacc atccaaaatc ccttctaccg tcgcttggat cgaagtgata
     1681 ggagtcttaa tatcatgcga caactgggca atcatcaagc ccttttctcg ttcgctttct
     1741 tccaaggaat caaagcttac ctgcaaatca tgggacatct cattaaaagt ttgccctaat
     1801 tgctgaaatt ctacaggacc ttgaacctcc aaatttgaag gaaaatcctt ggccgctacc
     1861 cgcttggcat gctccttgag tttgcccaac gacgtaaaga ctggcaatag gagaaagaga
     1921 ctaatcccag caccgacaag gctggcaacg atggtcatcc caagcaaaaa gtaaatctcg
     1981 cctttcgcaa tcagcatttt ttgaacagcc caaaaaacaa ccaaaatggt taagagggtt
     2041 gaaataatat atccaaccaa aatataactt tttagtttca tgtttgtcct ctcggtttct
     2101 ctatcttata tcccaacccc caaactgtct taatagtggg cgtttggtca ctactatatt
     2161 ttgccagctc ctgtcgaaga gcatggatat gcacattcaa ggtattggtg tcatccacgt
     2221 agtcttcttt ccagatcttt tcatagaggt ctgtcttgga gaaaactcgc tctggattgc
     2281 tagctaaaat ccacagcaat tcaaatgatt taacagttaa atccagcatt tcttctccta
     2341 tttgaacttc atgactacta tgattcattt ttagattgcc aagggaaatc agctctgttt
     2401 cgcccccacg atgaaggcgg cgcaaaatat tgtggacacg caaaaccagc tcacgtgggc
     2461 taaaaggctt agcaataaaa tcatctgctc ccaagctcag gccgtaaatc ttgtcctgtt
     2521 cactggtctt agcagtaata aataggaaag gctgctctgg tgataagtat tgaacctcac
     2581 tgattaaatc gtaaccatcc atccgaggca tcatgacatc tgtgataatc aaatcaatcg
     2641 gttttttctt gaacagctct agcgcttcca gtccatcatg ggctaccaag acctgataac
     2701 ctgcctgaat taagtatctc tgatgaatat ctgtgatttc tacctcgtcg tcaacgagta
     2761 aaattgtctt tcccatctgt ctctcctttg ataaaaacag tgctatactg ctttaagtat
     2821 aacactattt ttattgtttt acgatgattt taatctgcta ttgaaagaaa gtcctgattg
     2881 ctgattgagt tgttaaacta atcttagtca gcccactatc catcatttat ttccttagct
     2941 gattagtttt cttctttttt gtttttcaaa cctagaccac cgagtaaacc tgcaagcgca
     3001 agcccaagga aaccaatact tgccattgat gtttgagtct caccagtatt tggtagcata
     3061 gctttatcct ctgacatcat cgtatcagac atcttgttag cagaagcagc catgttttca
     3121 cctgccatcg tgttggtaga acttgtcatg gtgtcagcag gcatgctatc tgtagtacct
     3181 gtagcatgat tgtgattcat cggagtcacg ccagaaccag agttagaagg tgataatgaa
     3241 ccatttgctg tgtctgatgt ttctttaaca tttatcttaa tagtgacttt tttagttgct
     3301 acgatgttgt ccaa
//
LOCUS       DAVHIT010000122         3215 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVHIT010000122 DAVHIT010000000
VERSION     DAVHIT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.124"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(136..390)
                     /locus_tag="VVN16_001948"
     CDS             complement(136..390)
                     /locus_tag="VVN16_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU5573256.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(410..2251)
                     /gene="typA"
                     /locus_tag="VVN16_001949"
     CDS             complement(410..2251)
                     /gene="typA"
                     /locus_tag="VVN16_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5573257.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2413..3138
                     /locus_tag="VVN16_001950"
     CDS             2413..3138
                     /locus_tag="VVN16_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU5573258.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDSGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
BASE COUNT          947 a          712 c          613 g          943 t
ORIGIN      
        1 aaatagattc cattaaagaa ttttattcaa gtttttccca ttcatattat ccaaacaaaa
       61 gagatggtga taactcaaaa ataaattgca atcttgtaaa attcctagca aaaaagagag
      121 ccaaaactct cttttttatc ttcttttact ttttttgact ggcatgagtg tgatgtctct
      181 cacactaaag taagctagga tcaacatggc tattgctagg aatatttctg ttggtaattg
      241 aaaaattttc agaaaagata gaaccaataa aatcaagagt gccactaaaa tacataccat
      301 agcgacgata ttgactgtcc ctttaatgct ttctggtgtc gcaaatacat agagtaggag
      361 cagtaaaatt cctaggacta aatagaccat ctttctctct ttctagctct tattcagctg
      421 attttttctt cttgttagct ttctcacgct ctgctttgtt aaggatttgt ttacgcaaac
      481 ggatagactc aggcgttact tccatgtact catcgtcgtt caagaactca agagactctt
      541 caagtgtcaa gatacgaggc gtcttgataa cagctgtttg gtccttagta gctgaacgaa
      601 cgttggtcat ttgttttgcc ttcgtgatgt taactgtcaa gtcattttca cgagagtttt
      661 cgccgatgat cattccttca taaacctcag tacctgggtt gacaaagatc gtaccacgtt
      721 cttcgataga catgattgag taagttgtag ccttaccagc atcgatagaa acaagggcac
      781 cacggtgacg tccaccaatt tcccctggaa tcaatggcaa gtactggtcg aaggtatggt
      841 tcatgatacc gtaaccacga gtcattgaca agaactcagt tgagtatcca atcaaaccac
      901 gcgctggaac aaggaagacc aaacgagttt gaccattacc agttgaaatc atatccaaca
      961 tttcaccttt acgttcagaa aggctttgga taacagaccc ttggtattct tctggagtgt
     1021 cgatttgtac acgttcaaat ggttcacatt taacaccgtc gatttctttt acgataactt
     1081 ctggacgaga tacttgaagt tcatagccct cacgacgcat tgtttcgata aggattgaca
     1141 agtgcaattc tccacgtcct gaaacagtcc atttatctgg tgaatcagtt gggtcaacac
     1201 gaagggaaac gtctgtttgc aattctgcct gcaagcgttc ttccacctta cgagaagtta
     1261 cccatttacc ttctttacca gcaaatggtg agttgttgac caagaaagtc atttgaagag
     1321 ttggctcatc gatgtgtagg attggaagag cttctactgc atctgtcgga gtgatggttt
     1381 caccgacaaa gatgtcttcc atacctgaaa cggcaatcaa gtcacccgct ttggcttctt
     1441 ggatttcacg acgttccaaa ccaaagaaac cgaagagttt tgtaacacgg aagtttttag
     1501 ttgtaccgtc aagtttagaa agggtaactt ggtccccaac cttaactgta ccacggaaga
     1561 cacgaccgat accgatacgt ccaacgaagt cattgtagtc caaaagtgac acttggaact
     1621 gcaaaggctc atctgagtta tctactggag ctgggatatg gtcgataatc gtgtcaaaga
     1681 ttggtgccat agtcgcttct tggtcagctg gatcatctga caatgaagaa gttccgttga
     1741 tcgctgaagc ataaaccact gggaaatcaa gctggtcgtc atctgcacca agctcgatga
     1801 aaagttccaa gacttcatcc actacttctg ctggacgagc tgatggctta tcgattttgt
     1861 taacaaccac gattgggaca aggtcttgtt ccaaggcttt tttcaatacg aaacgagttt
     1921 gtggcatggt tccttcatag gcatctacga ccaagacaac accgtcaacc attttcatga
     1981 tacgctcaac ttctccacca aagtccgcgt gtcctggtgt gtccataatg ttgatacgag
     2041 ttccgttgta agcaacggca gtatttttag caaggatggt aatcccacgc tctttttcga
     2101 tatcgtttga gtccatagca cgctctgcca attcagtacg tgcatcaagc gtttctgatt
     2161 gtttcaataa ttcgtcaacc agggttgttt taccgtggtc aacgtgggcg ataatcgcaa
     2221 tgttacggat atcttctctt aattttgtca tgatttcctc tataatattc aaaatttatt
     2281 ttctaactga acgattatac cataatttca agtaaataac ataactcaag caagtgtaaa
     2341 tgttttcact ccacttttct tttcacgtca agccttttca aagcaagcga cttatgataa
     2401 gataggcaca gtatgcgttt agataattta ttagctcaag aaaaaatcag ccgaaaggcc
     2461 atgaaacaag ccttactcaa aggggaaatt ctagtcgatg gttgcccagc ccgatcccta
     2521 gctcaaaata tcgatacagg actacaagaa ctcctttttc aggatcgaat cattcaaggc
     2581 tatgaacaca cctatcttat gcttcataag cctgctggtg tcgttacagc caacaaagac
     2641 aaggaacttc cgaccgtcat ggacctgctt ccatctgaca tccagtctga caagctctat
     2701 gccgttggcc gactggaccg agatacaacg ggactcctcc tcttgaccga tagcggtccc
     2761 ttgggctttc agctcctcca tccccaatat catgtcgata agacttacca agtccaggtt
     2821 aatggacttc taacacctga ccatatccaa gcctttcaaa agggaattgt ctttttagat
     2881 ggtactgtct gtaaacccgc aaaactagag attctatctg caagtccctc cctcagtcaa
     2941 gcctctatca ccatttcaga aggaaaattt catcaaatca agaaaatgtt cctctcggtt
     3001 ggtgttaagg tgactagcct caaaagaatc caatttgggg acttcacatt gaacccagat
     3061 ttagcagaaa gtaactaccg ccctttgaac caaaaagagt tacaaatcat taaaaactat
     3121 ttagagatga gtcgataaaa caaaaaaagc tttaaaacta aagctttttt cttttattta
     3181 ccgaaaaatt aaggcgattg ctacaatcca gttaa
//
LOCUS       DAVHIT010000123         3112 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVHIT010000123 DAVHIT010000000
VERSION     DAVHIT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.125"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(356..1522)
                     /locus_tag="VVN16_001951"
     CDS             complement(356..1522)
                     /locus_tag="VVN16_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5573259.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(1535..2274)
                     /locus_tag="VVN16_001952"
                     /pseudo
     CDS             complement(1535..2274)
                     /locus_tag="VVN16_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2296..3093)
                     /locus_tag="VVN16_001953"
     CDS             complement(2296..3093)
                     /locus_tag="VVN16_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009444206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5573260.1"
                     /translation="MKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPVVYFHDGQNV
                     FYSKESFIGYSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQESPIPEQQF
                     GGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGLEYQDQIGCL
                     GVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGNIKQAYIDSS
                     LCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
BASE COUNT          903 a          683 c          610 g          916 t
ORIGIN      
        1 gtatatgata ctgactctgt cagttctatc cacaacctca aaacagtgtt ttgagcaacc
       61 tgcggctagc ttcctagttt gctctttgat tttcattgag tattaataga cttgtttggt
      121 atcctgaaaa ctatatcgta taggtgtcgt catggatcct tttagctgat ttaaggatac
      181 gaaaacacaa tactatgact aaattgaaat gttaaaattc aatttctagc cacctaatcc
      241 tgcttcaaat agttatcgaa caagttttat gatagctacc ttgttaggat gctcttattt
      301 tttaccagac aaaacaaaag gattaaggga gtgaatttcc ttaatccgat agttcttatt
      361 cttgacgttg tccgaaatca gcaatcatct gatccatttc ttgtcgactc ggagttgtca
      421 gcatatacag gtaatctcct tgaagttccg cgaaggcagc tggcatgact tttttaacct
      481 tgaactgctg gctatatttg gcaagcaaat cctcttctga ataaacatag tgagcatttg
      541 cacggcgaga agtagccaaa caatactgag tttcaaagtc tgacgccggg aactcctctc
      601 ctgcgacaat agctgcatag ccacgataaa ggtccaagga atgagcaaag ttataaacat
      661 caatggtaaa accacctgca gggcggttat tgtactcgat ggtaatataa tcgtccccct
      721 cacggaagaa ctcaatatgg aaaaaccgtt ctttcatacc aaattctttg acaattgctt
      781 ccccatactt gcgcagttta ggatccatat ccttgagcac ataataagaa ttgtccatct
      841 tataaatcat gaggtcaagc ggtgtatagg cgtagtcaaa ggttgttgag aagacaatct
      901 ttccatcctt gtccacgagc ccgtcaaagg tacagatttc gctggaagtg acaaattttt
      961 caaagaaata aagggttgaa tggtcccatt cttgcttgaa gtgattgata tcgtcttctg
     1021 tctcaagttt aaaggttgcg gctgctccca ctccattatc aggtttggca atcattggaa
     1081 gaccgatttc tttcactgct tgatcaacat ctgcttccgt cttgataaca gctccaggta
     1141 ccacaggaac acctgctttt ttgaaaagtt tcttcatttc agacttatat ttcgtctttt
     1201 tgagatcctc tggtttggca ccaaaaacat tgaattgttc tctgagtgtt gcgtctagct
     1261 caagccagta ttcattgtga gactcgatgc ggtcaattgg accatgttta taaaagagaa
     1321 aagcaactgc acgtttgact tcatctatgt tctcaagatt atcaacacga aaatactcgg
     1381 tcaagctatt gcgcaagggc tcatccaatt gctcgtaaga ctcttgacca attcccaaga
     1441 ctgtgatgcc tttattagct agttcgatgg taaactgttg aaagttttgt ggatagtagg
     1501 gagaaataac aaggtaattc ataggtaact ccttttataa atagagatta ccgaggaaat
     1561 aaggcatttg tttacgccac cattcccagt catgggcgac atcatgtccc cattcagcaa
     1621 accaggctgg aatttgtttc ttgtcaaagg cttctttgag cttgtaaaag gatggcaaac
     1681 catcttgttc ccaggctcca agacccgtac acagcacaat ctctgcctga cggtaacggt
     1741 caataaacca gccgtcgttt tggttccaaa tataatctac tggcgagttt tggtaaatag
     1801 catcatcgtt gtagtaatca ccgacaaaga aacgtgcgtc gtaaacacca ctgagagcaa
     1861 tcactttggt aaagacatct ggatgttgga ggaagaaatt gagtgcatga taggctccca
     1921 tagagcaacc tgtcgtcatc atgccatcaa accaacctga cttgtgcttg ataaaagaat
     1981 ggcctcctca atcacataac attcgtaggc acggtgcatt tccgcttggt catgagcatt
     2041 tttccaagta gccaaccagc tctcactatc caaactagat agggtaaaga actggacacg
     2101 gccttcctcg ataaaggaag cacaggcatc aatcatgcca aaatcatagt attcgttgtg
     2161 actaccacct gatgaagcaa agaccacaac tggaatccca ccatgtccat aacggttaag
     2221 gtacatttca cggttaggat gaccactcca gtggctaaga tgttcaatat gcatttcgtt
     2281 ttttcctttc ttaacttacc atttttctgc aaaaaatctc agacaatctg gtagattttc
     2341 tgaccaaggg atttcactat ggatggcacc agactgaact tttagcacaa gattatccag
     2401 atgtactccc cctgctatca aatcatggta atagcaaagc gacgagtcga tataggcttg
     2461 tttgatattg ccatccatca aggtcttgtc tgtatcatct gcttcttctg ttcctacata
     2521 gatgaagatg cgctggtcag gcgatagttt ctggcactcg atatagcggt taaaggcttc
     2581 ttggtggagc cagtttgcag atgaaaaaac gcccaagcaa ccaatttggt cttggtattc
     2641 caaaccgata aactgggtaa tattgcctcc tagtgaggaa ccaatcatag ccgtatgctg
     2701 gcagtctgct tttgtacgat aggtctcatc gataaaaggc ttgaccacct ccatgacaaa
     2761 ctcagcatac tccacaccct taccaccaaa ctgctgctct gggataggag attcttggaa
     2821 cttccaagcc gcatactcat tcattcgccc cataccatca ttgtcaatag caacgacaat
     2881 catgcgactg atatccggat ttcgtttgat agctgggata atcttccatg aatatccaat
     2941 gaaagactct ttgctataaa aaacattttg cccgtcatga aagtatacaa caggatagga
     3001 acggtctgta tctttctcat aatctttagg aagaagaata cgtacacggc gctccttacc
     3061 tgtataagga accttgagtt tgtgttcttt catttttaga taaaagtagg at
//
LOCUS       DAVHIT010000124         3085 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVHIT010000124 DAVHIT010000000
VERSION     DAVHIT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.126"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(191..919)
                     /locus_tag="VVN16_001954"
                     /pseudo
     CDS             complement(191..919)
                     /locus_tag="VVN16_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1179..1490)
                     /locus_tag="VVN16_001955"
     CDS             complement(1179..1490)
                     /locus_tag="VVN16_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573261.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1505..2791)
                     /locus_tag="VVN16_001956"
     CDS             complement(1505..2791)
                     /locus_tag="VVN16_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU5573262.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          924 a          676 c          541 g          944 t
ORIGIN      
        1 ttcaaaatat ctaagacatc gcttctttgt ttaatttgat aggctttaat acctaagctc
       61 cgactttatc acttaaaaaa taaaaaagga cagctaatct cttgcgcatc aagggatttc
      121 gccattcttt aatctcgttt aagtgttgta tatggagctt tgcataattt tttaaaacta
      181 aacaactaca ttaagcacga actgtgacgc tggttccatc ttccttataa agattgataa
      241 gcccctcttt aagagctcga accatgtctc cagcttgaac aggttgtggg actttaatag
      301 tcaatagttc cattggattt ggagcgcggt cgattttatt gcctttggca tcatgcaaat
      361 cttcaatata ggtttcaaaa tgacggaaac ctggaccata aaactcaact tggtcccctt
      421 cgttaatgac gtttcgttga cgaatagttg ctgtttgtgc cgcatcatca taagaaacca
      481 cttcagcgac aaacttgtac tcagggattt tacgacgagc accaaacaac tgctcatttt
      541 cagatggtgt accatagtaa aatcctgtag ccagttcacg ttgggcaacc ttccacatct
      601 cgtccaccaa gtcttgtttg atagcttcaa acttttcagg actttcaaga taggcatcca
      661 cagccgcctt gtaacagttg gttactgttg atacatagtg aatagactcc atacgtcctt
      721 cgatttttag actgtccaca ccattttcaa tcatatctga gatatggtca atcatagaca
      781 tatcaacggc tgacattgaa aattcttctg gaatctcacc ctgcaaactc ttacgttctt
      841 tcccaaatgg catatcgtaa aggtcgtact tccaacggca tgactgagaa catccaccac
      901 ggttggcatc acgcatactc tagacggatt gttttgacaa ttctaacctt ttagactttg
      961 cataataact gttaaacagt cgaaaattac cccagtttta acaagtagtc aaaataccag
     1021 ataaagtaaa aaggggctag gaaaaaagtc tttaaaatct taaaaacgca tagtatcagg
     1081 tattgaacaa tcttgatatt atgcgtttta ttgtgggaag atttacttca ttttctcttg
     1141 gaattgagtt ttctcctact tcaaaactag atgatttgct atttaaaata gcgttcctta
     1201 tgattttggc aacctggatt aaagcgagaa aaacaaaaga ggcaacttaa gctttcttta
     1261 tattgcttat atgttaatag ttttagacaa acaccacata aaataggctt atcctgtatc
     1321 aaagataagg gataaggctc aaaagggtga ttttctaatg aatcatgaca ccgataacaa
     1381 gcataatact ttttacaatc atagcattgt aaagcaataa tatccagctt gctatggtaa
     1441 tgaatgcatc ttgtttgctt atccactaag aaaccctgtg cctctatcat ttccttttcc
     1501 tttcttaagc tcgaactgtg acgctggttc catcttcctt ataaaaattg atgagtcctt
     1561 cttttaatgc acgaaccatg tctcctgatt gaacgggttg tgggacttta atagtcaata
     1621 gttccattgg atttggagcg cggtcgattt tattgccttt ggcatcatgc aaatcttcaa
     1681 tataggtttc aaaatgacgg aaacctggac cataaaactc aacttggtcc ccttcgttaa
     1741 tgacgtttcg ttgacgaatg gttgctgttt gtaccgcatc atcataagaa accacttcag
     1801 cgacaaactt gtactcagga attttacggc gagcaccaaa caactgctca ttttcagatg
     1861 gtataccgta gtagaaacct gttgccaatt cacgttgggc aaccttccac atctcgtcca
     1921 ccaagtcttg tttgatagct tcaaactttt caggactttc aagataggca tccacagccg
     1981 ccttgtagca gttggttact gttgatacgt agtgaataga cttcatacgt ccttcgattt
     2041 ttagactgtc cacaccattt tcaatcatat ctggaatgtg gtcaatcata gacatgtcaa
     2101 cggctgacat tgaaaattct tctggaatct caccctgcaa actcttacgt tctttcccaa
     2161 atggcatatc gtaaaggtcg tatttccaac ggcatgactg agaacatcca ccacggttgg
     2221 catcacgcat actcatgtgg tttgaaagag tacaacgtcc agagtatgaa atacacatag
     2281 ctccatggac aaaggcttca atttcaacat ctgtacgttt gcggatctca gctaattctt
     2341 ccattgaaac ctcacgcgct aaaacgacac gagtcaagcc tagctctttc cagaactcaa
     2401 gggtttcata gttagtggca ctggcttggg tagaaaggtg gatttcaagg cctggtgctt
     2461 cagtcgctgc aatcataatc aaggctgggt cagatacgat aactgctgca atcccgatat
     2521 cacgcagttt acggaaccac tcaccagcac cagcttcatt tccttcgtgc ataaccatat
     2581 tagccgctac atagaccttg gcaccatact tggccgcaaa ctgcacgcct tcttccatct
     2641 gttcgaaagt aaagtttccc gcacggctac gaagaccata ggcctgacca ccgataaaga
     2701 cagcatctgc tccatactga acagctacct ttagcttctc taaagtccct gcaggtgata
     2761 aaacctcagg acgttttaat gtttttgtca tgatttctcc tatttgcaat ataaaagttt
     2821 gacatttttc cttctcattt tatagtaaat aaaggataaa atcaagccta gacggattgt
     2881 tttgacaatt ctaatctttt agactttgca taataactgt taaacagtcg aaaattaccc
     2941 cagttttaac aagtagtcaa aaagtctaaa aaagtaaaaa ccagtatccc aagatactga
     3001 ctcgtaaaaa tacataaatt agtccttaga tgactactcc cactctgttg ctcctataat
     3061 ggaaaaggat tgatacagtc tagaa
//
LOCUS       DAVHIT010000125         3042 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVHIT010000125 DAVHIT010000000
VERSION     DAVHIT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.127"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(154..309)
                     /gene="blpC"
                     /locus_tag="VVN16_001957"
     CDS             complement(154..309)
                     /gene="blpC"
                     /locus_tag="VVN16_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU5573263.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDILYSLNIIKHNNTKG
                     LHHPIQL"
     gene            complement(366..1727)
                     /locus_tag="VVN16_001958"
     CDS             complement(366..1727)
                     /locus_tag="VVN16_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU5573264.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDTIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLGNNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            complement(1738..2996)
                     /locus_tag="VVN16_001959"
                     /pseudo
     CDS             complement(1738..2996)
                     /locus_tag="VVN16_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          861 a          503 c          618 g         1060 t
ORIGIN      
        1 ctttttgtgt ttttttctac acttatacca taaaggggaa actctttttt gtctagtaaa
       61 aaacacccat tgggtgaaaa aagaaaccat ccaggatcta agctaaggca aggattctgg
      121 atggttttta gattaggggt gaataattgg ggtttatagt tgtattggat gatgaagtcc
      181 tttcgtattg ttatgcttaa ttatattaag gctgtataag atatcttccc acagtcctcc
      241 acctgtaatt tggttgagtt cggtagttgt tagttcttga aatgaagtta ggttttgttt
      301 cttatccatg ttatgattct cctttttgat aagataataa atagttatag agtgttatct
      361 gaaaattaat cagaatgggt taaaatttta tctttgaaat aatcaaaata tgttttcttt
      421 gcagttacac tagtgacgcg accttgtaag ccatattgga tgagtttact atcctcatta
      481 gatagttttg caagagcggt taatttaaag agattacctt gctctgttct ggtaggagtt
      541 tgatcaattg tctgaagttg gccgatgatg gtggtgccgt gatttccaat cttctccagt
      601 tttaatctta cagtttgtcc tttatctagt agaggtagat agtcagaaga tacgtagtaa
      661 gtgattagta cttctcttgt atctgtgatg acagggaata tttgagcaat ttctgtacca
      721 gttggaattc tatttttacc ttcaaattcg ctgttcagat gaacgatacc tttacttgga
      781 gcggttaagg tattgtttcc taagcgctgc gttgcttgat ctagttgtac ttttaattct
      841 gttaattgat tctccacggt tagttgttgc tgtgaggctg tttgtaaaaa ctgagtgcgg
      901 agtacttcaa ttttggttgc taaactatta tcataagttg ctacacttcc gataccagct
      961 tgctgaattt tgaggcttgc gatagatgat tccagacctg caatactttg attaatttga
     1021 gataaaaatg gctcctctgc cgttccttgt gtttgttctt gagaggctat aagataacga
     1081 tttaaaattg actggtgcgg attgccagtt ggtaagcgag ctcttttatt tacgatagta
     1141 tctctcagct cttgatattc tccaatttgt tgttgaactt ttgtaatttc ttgttcgatg
     1201 gctgatgaac tgctattggt aagattagct tgatttgaaa cttcagtgtt agtctttgag
     1261 atacccagtt caatatcatg ggattgttta gtaaaattca taaaagtatt atggtagcca
     1321 aactcatcct cgctagaaaa aagatcagtc gctttttcta agctttgttt caagattcca
     1381 agtccttctt tttgcttctc aagtctttgt aattgagttt ctaaggcagt tttctgactc
     1441 tcttccattg tttcagagta tttgatgagt aagtcacctt tctcaactac ttgatttgcc
     1501 actaaatgat tagcaaggat aggattatca ctggtcgact gaatagatgc aatgacactt
     1561 gtaggggtga tttccccttg ggaagtaaca gtaatttctt ttgtggcaac aagggagaaa
     1621 atcaagatga aagtaaataa taatgaaaga ggtatgatta atactgtcgc atagttatgg
     1681 taacgtctct gataaaactc gacgcttcta aaaagattag gattcatgac attctcctta
     1741 tttattgaac agatgatggt agaagccttg cgcctgcatt aactcttggt gactaccaac
     1801 ttcaatgatt ttcccctggt caagaacaat gacacggtta gttcgttcgg ctatactgag
     1861 acgatgggct acaaagagaa tggttttatc agttagggac ataagattat ctataacttt
     1921 tttctcagtc aagacatcaa gaccgctagt agcttcgtct agtattaaaa caggagattt
     1981 agttaaaaga gcacgagcga gggctattcg ttgcttctgt cctcctgata gaccagctcc
     2041 atcagagagc tgagtttgat agcccatagg cattctttca atgtcttgac ggatttcagc
     2101 taattcacaa gctcttagaa tatcttcttg gctaatcata tgattaccgc ccaaggttaa
     2161 attttccaag atagagccat taaagatata ggcttgttgg ggtaggtaat taatatgacg
     2221 gcgcaagact tttttatcaa tgtttttaat atcctgatga ttgatggaaa tatgcccttt
     2281 gtagggttca aagaaattga caatcatttt ggctaaagtt gttttaccag aaccactaac
     2341 tccaactagg ctaaccttat ctccttgttt aatcgtgaga ttaatatctg ttaaggtatc
     2401 tcgtccaaaa ccatacttat aagaaaggtc atcaaattca atatcgccca tcaaaaaatg
     2461 tgaatgaaca gggttttctt gaacttgaaa ttcagattcg actagataga cttcgttcaa
     2521 acggttatta gcgaccttcg cagattggag tttggttggg gttgatagct gggtttggga
     2581 gctagaaaaa tagctactcc agtccattca gagaaaaagc gttcttttga cattttggtg
     2641 atttttacag aagggtcagg atcaccaata accagatagt ctttctttgt ttgatagaca
     2701 acatagtaat gttggagttt ttcttctttg ttaacgtgaa cgataaatgg ataggggaca
     2761 tcactcatgt caaagagagt tttatctgct tgaacaggtc ttgtttcaaa gcccatttca
     2821 tcagcggctt ttacaatgcc aagagcagtc gttccttctt tattggtctt tgcaagttct
     2881 ctcaagtgag ctagagaaaa atctgaacca tagaatttag caatcgaggc taaggcagca
     2941 acaccgcagt ctctcgcatc tatttgagga acaaatgtac gtttataaga agtcattggc
     3001 aattcctttc atatagtaga taggtatatt atgccataaa aa
//
LOCUS       DAVHIT010000126         2919 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVHIT010000126 DAVHIT010000000
VERSION     DAVHIT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.128"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1950
                     /gene="ftsH"
                     /locus_tag="VVN16_001960"
     CDS             <1..1950
                     /gene="ftsH"
                     /locus_tag="VVN16_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5573265.1"
                     /translation="QNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYTELV
                     QEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTILPA
                     DTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGGGNG
                     RNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGARIPA
                     GVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAA
                     PAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNRSDV
                     LDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFVGAD
                     LENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHEAGH
                     TIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGRVAE
                     EIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKSISE
                     QTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYETGK
                     MPEAVEEESHALSYDEVKSKMNDEK"
     gene            2072..2551
                     /locus_tag="VVN16_001961"
     CDS             2072..2551
                     /locus_tag="VVN16_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5573266.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLADDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            2645..2716
                     /locus_tag="VVN16_001962"
     tRNA            2645..2716
                     /locus_tag="VVN16_001962"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2678..2680,aa:Glu,seq:ttc)
BASE COUNT          991 a          464 c          670 g          794 t
ORIGIN      
        1 caaaataatg gtttaattaa aaatcctttt ctatggttat tatttatctt tttccttgtg
       61 acaggattcc agtatttcta ttctgggaat aactcaggag gaagtcagca aatcaactat
      121 actgagttgg tacaagaaat taccgatggt aatgtaaaag aattaactta ccaaccaaat
      181 ggtagtgtta tcgaagtttc tggtgtctat aaaaatccta aaacaagtaa agaaggaaca
      241 ggtattcagt ttttcacgcc atctgttact aaggtagaga aatttaccag cactattctt
      301 cctgcagata ctaccgtatc agaattgcaa aaacttgcta ctgaccataa agcagaagta
      361 actgttaagc atgaaagttc aagtggtata tggattaatc tactcgtatc cattgtgcca
      421 tttggaattc tattcttctt cctattctct atgatgggaa atatgggagg aggcaatggc
      481 cgtaatccaa tgagttttgg acgtagtaag gctaaagcag caaataaaga agatattaaa
      541 gtaagatttt cagatgttgc tggagctgaa gaagaaaaac aagaactagt tgaagttgtt
      601 gagttcttaa aagatccaaa acgattcaca aaacttggag cccgtattcc agcaggtgtt
      661 cttttggagg gacctccggg gacaggtaaa actttgcttg ctaaggcagt cgctggagaa
      721 gcaggtgttc cattctttag tatctcaggt tctgactttg tagaaatgtt tgtcggagtt
      781 ggagctagtc gtgttcgctc tctttttgag gatgccaaaa aagcagcacc agctatcatc
      841 tttatcgatg aaattgatgc tgttggacgt caacgtggag tcggtctcgg cggaggtaat
      901 gacgaacgtg aacaaacctt gaaccaactt ttgattgaga tggatggttt tgagggaaat
      961 gaagggatta tcgtcatcgc tgcgacaaac cgttcagatg tacttgatcc tgcccttttg
     1021 cgtccaggac gttttgatag aaaagtattg gttggccgtc ctgatgttaa aggtcgtgaa
     1081 gcaatcttga aagttcacgc taagaacaag cctttagcag aagatgttga tttgaaatta
     1141 gtggctcaac aaactccagg ctttgttggt gctgatttag agaatgtctt gaatgaagca
     1201 gctttagttg ctgctcgtcg caataaatcg ataattgatg cttcagatat tgatgaagca
     1261 gaagatagag ttattgctgg accttctaag aaagataaga cagtttcaca aaaagaacga
     1321 gaattggttg cttaccatga ggcaggacat accattgttg gtctagtctt gtcgaatgct
     1381 cgcgttgtcc ataaggttac aattgtacca cgcggccgtg caggcggata catgattgca
     1441 cttcctaaag aggatcaaat gcttctatct aaagaagata tgaaagagca attggctggc
     1501 ttaatgggtg gacgtgtagc tgaagaaatt atctttaatg tccaaactac aggagcttca
     1561 aacgactttg aacaagcgac acaaatggca cgtgcaatgg ttacagagta cggtatgagt
     1621 gaaaaacttg gcccagtaca atatgaagga aaccatgcta tgcttggtgc acagagtcct
     1681 caaaaatcaa tttcagaaca aacagcttat gaaattgatg aagaggttcg ttcattatta
     1741 aatgaggcac gaaataaagc tgctgaaatt attcagtcaa atcgtgaaac tcacaagtta
     1801 attgcagaag cattattgaa atacgaaaca ttggatagta cacaaattaa agctctttac
     1861 gaaacaggaa agatgcctga agcagtagaa gaggaatctc atgcactatc ctatgatgaa
     1921 gtaaagtcaa aaatgaatga cgaaaaataa ccctgagaga ggctggagcc tctctttttt
     1981 gtgcagttta ggagctaaag ggaacagaat ggagaaaatg gaacaaatgt gttttctaat
     2041 ctgttagact gtatctagaa aggggaaaat tatgattaaa gaattgtatg aagaagtcca
     2101 agggactgtg tataagtgta gaaatgaata ttaccttcat ttatgggaat tgtcggattg
     2161 ggaccaagaa ggcatgctct gcttacatga attgattagt agagaagaag gactggcaga
     2221 cgatattcca cgtttaagga aatatttcaa aaccaagttt cgaaatcgaa ttttagacta
     2281 tatccgtaag caggaaagtc agaagcgtag atacgataaa gaaccctatg aagaagtggg
     2341 tgagatcagt catcgtataa gtgagggggg tctctggcta gatgattatt atctctttca
     2401 tgaaacacta agagattata gaaacaaaca aagtaaagag aaacaagaag aactagaacg
     2461 cgtcttaagc aatgaacgat ttcgagggcg tcaaagagta ttaagagact tacgcattgt
     2521 gtttaaggag tttactatcc gtacccatta gtaagtcatg caaaaaaaat gaaaaaaatt
     2581 agaaaaagta gttgacaaag tttgaaaagg ctgtataata gtaagagttg aaaatagcaa
     2641 ctcaggtccg ttggtcaagg ggttaagaca ccgccttttc acggcggtaa cacgggttcg
     2701 aatcccgtac ggactatggt atgttgcggt tggaacactt gatgaaaaaa agtttaaaaa
     2761 agcttaaaaa tcttcaaaaa agtgttgaca agcgaaagcg gctgtgatat actaatatag
     2821 ttgtcacttg agagaagcaa gtgacaaaga cctttgaaaa ctgaacaaga cgaaccaatg
     2881 tgcagggcgc tacaacataa gttgtagtac tgaacaatg
//
LOCUS       DAVHIT010000127         2898 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVHIT010000127 DAVHIT010000000
VERSION     DAVHIT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.129"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VVN16_001963"
     CDS             complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VVN16_001963"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU5573267.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYYQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1400..2176
                     /gene="recX"
                     /locus_tag="VVN16_001964"
     CDS             1400..2176
                     /gene="recX"
                     /locus_tag="VVN16_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU5573268.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDENIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2265..2798
                     /locus_tag="VVN16_001965"
     CDS             2265..2798
                     /locus_tag="VVN16_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU5573269.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          925 a          532 c          526 g          915 t
ORIGIN      
        1 ccttttttaa acttttttta ttaattttac aacagcttca gttcgagctg tatgtgggaa
       61 catatcgacc gactggatat aatgaagatc atagacttct actaagcgta ccaaatcacg
      121 agccaaggtc gaaacattac aagaaatata aaccattttt tctggtacat aagtaagaat
      181 agtatctaat aacttatcat ccagacctgt acgtggtggg tcaacaatca aagcatctgc
      241 tcggtagcct tccttgtacc aacgaggaat aatctcttct gccgttccag cttcataatg
      301 agtattgtca aatcccattc ttttagcatt tcgcttggca tcttcaatag cttctggaat
      361 aatatccata cctctgagtg tttttacttt ctttgcaaag gcaaatccaa tcgttccaac
      421 tccacaataa gcgtcaatca aatggtcttc tttatcaaca tccagcgctt ttactgcttc
      481 gctatagagg acttctgttt gctcaggatt tagttgataa aaagctcgag gggatagtga
      541 aaattcataa ttgagtacac cttcttgaat actctcttgc ccccagataa tctctgtctt
      601 ttcaccatat atctcactgg ttttagctgt atttgtatta acagctactg tcacaacttc
      661 tgggaaatct ttaaccaact cttttaccaa ttgagttaaa ttaagctggc ggtttgtaac
      721 aataataatc tgaacctgtc cggtctttct cgcgcgtcgg accataatag tacggacacc
      781 tagaactttt ctctcatccg tgattggaat ctggtaataa gtaagtaatt ctgctaagcg
      841 attagcaatc acttgggttt ccttatcttg taccaggcag tctttcaact ctactaaata
      901 gtgagagttt tgtgcatata agcccgcctt gacctgattt ttaaattttc gagtctgaaa
      961 ttgtaactta gctctgtaat attttggttc ctgcattcca atagttggac gaatttcata
     1021 attttcatat cctgcaggag caaatttttt cagcgcttga tgaagtaagt ccgtcttgaa
     1081 ctccagctgc ttatcataat gcaggtgcat gatttggcag cctccgcatt cattataaat
     1141 agtacaagat ggcacaattc gaaatttaga cttcttgttg accttcagta attttgcttc
     1201 aacaaagttg cgtctaatag aagtaatctg acaatagata tcttcgcctt tgagagctcc
     1261 tggtacaaag actaatgttt tttggtaaaa gccgattccc tcaccgttaa ttcccatgcg
     1321 cttgattttt aatggtattt tttgtttcac tttcagattc atacccctat cttatcacat
     1381 tttgagttat aatagagcta tgaaaatcac aaaacttgaa aagaaaaaaa gactctatct
     1441 gatggagctt gataatggcg acaaatgcta tatcaccgaa gatacaattg ttcgttttat
     1501 gttatcgaga gataaggtga taagcgaaga ggaattgaaa gagattcaag actttgctca
     1561 attttcttat ggtaagaatc tggccctcta ccacttatcc tttaaagcac gcactgaaaa
     1621 agaagtcaga gaatatctga aaaaatacga tattgatgaa aacatcgttt ctcaagttat
     1681 tgctaatctt aaagaagata agtggattaa tgatggtcag tacgcttatg ctatcatcaa
     1741 taccaatcaa ctttcaggag acaagggtcc ttatgtgctg actcagaaac tagctcaaaa
     1801 agggatttca aaatctacta tagaagagaa cttgaaagaa tttgattttt ctgaagttgc
     1861 tcaacgtgta gctaataaac tattgaaaaa atatgaggga aaacttccag ctcgtgccct
     1921 gcaagataag attatccaga acttgactaa caagggcttc tcttattctg atgctaaaat
     1981 tgcctttgac gacttagata gccaagttga ccaagaaacg actcaagaac tcatcttcaa
     2041 ggaacttgat aagcaatata ctaagtatgc tcgaaagtat gaaggatacg aactgaagca
     2101 gcgtttaact caagttttag cacgaaaggg ctacgatttt tcggatatag caagcgctct
     2161 tagagaatat ctttaatatt tccaagtgaa attcacagat tttaggtaat tttatggtac
     2221 aatagtaaat gataaactta taaattgtag aaagttggtt agttatgaag cttccaaaag
     2281 aaggcgactt tattacaatt caaagttata agcatgatgg gagtctccac cgaacttggc
     2341 gggacaccat ggtactaaaa acaacagaaa acgccattat tggtgtcaac gatcatacac
     2401 ttgttaccga aagtgatggt cgtcgttggg tcactcgaga accggctatt gtttactttc
     2461 acaagaaata ttggtttaat atcattgcca tgattcgtga taatggaact tcttactatt
     2521 gcaatatggc tagcccctac tatctggatg aagaagcact gaagtatatt gattacgatt
     2581 tggatgttaa aatttttaca gatggggaaa aacgtctctt ggacgttgaa gagtacgagc
     2641 gtcacaaacg caaaatgaat tattctgatg acttggacta tattttaaaa gaacatgtca
     2701 aaattcttgt tgattggatt aacaatggac gtggtccttt ctcagaagcc tatgtaaaca
     2761 tttggtacaa gcgctatgta gaactaaaga atcggtaaag ttgtcaaagg tcaggaacaa
     2821 aatcctgacc tttttttggt agtataaaat aaattgtgta aacacaaaaa ggaataaatc
     2881 cattatagca gagttaca
//
LOCUS       DAVHIT010000128         2765 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVHIT010000128 DAVHIT010000000
VERSION     DAVHIT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.130"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..865
                     /locus_tag="VVN16_001966"
     CDS             <1..865
                     /locus_tag="VVN16_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5573270.1"
                     /translation="DSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDI
                     DLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQIL
                     IDGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCT
                     DFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKEAPILILDEITASLDVNNEKK
                     IQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLI
                     EKTKMAEEFIY"
     gene            876..1460
                     /locus_tag="VVN16_001967"
     CDS             876..1460
                     /locus_tag="VVN16_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU5573271.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLIIYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1460..2155
                     /locus_tag="VVN16_001968"
     CDS             1460..2155
                     /locus_tag="VVN16_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU5573272.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2143..>2765
                     /locus_tag="VVN16_001969"
     CDS             2143..>2765
                     /locus_tag="VVN16_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573273.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDR"
BASE COUNT          987 a          358 c          539 g          881 t
ORIGIN      
        1 agactcttta gatgcatcta aagagggctt gatggaaata ttttatttat cgcccaaaat
       61 agaaagatta aaagaaattc aaaatcaaga tttacaagaa ggcgatgact atagcttaaa
      121 aaaatttgat attgatctaa aagatgttga gtttgcctac aataaagacg caaaagtttt
      181 aaacggtgta agttttaaag ctaagcaggg agaggtcact gctttggtag gtgcaagtgg
      241 ctgcggtaaa acaactatct tgaaacttat atcaagactt tatgattatg acaagggaca
      301 aatcttaatt gatggcaaag atataaagga aatatcaaca gaatcccttt ttgataaggt
      361 gtctattgtt ttccaagatg tggttctctt taatcaaagc gttatggaaa atattagaat
      421 cggtaagcaa gatgcaagtg acgaagaggt taaaagagca gcaaaacttg caaattgcac
      481 agattttata gaaaaaatgg ataaaggttt cgatacagtt attggtgaga acggagctga
      541 gctatcagga ggagaaagac aaagattatc aatagccaga gccttcttaa aagaagcgcc
      601 gatattgatc ttagatgaga taacagcaag ccttgatgtt aacaacgaga aaaagattca
      661 agagtcttta aataatttag ttaaagataa aactgttgta atcatttcac atagaatgaa
      721 atccatagaa aatgcagaca agatagtagt tcttcaaaac ggaagagtag aaagcgaagg
      781 taagcatgaa gagcttttac aaaaatcaaa aatttacaaa aatttaatag aaaagacaaa
      841 aatggcagaa gaatttattt attaggagga ctacaatgga taataaaaaa ttaaaagtaa
      901 aagatttagt aagtatcggt gtttttggcg taatttattt tgccttcatg tttggagttg
      961 gtatgatggg cttgattcca atattgttct taatataccc gacagtatta gccatagttg
     1021 caggaactgt tgttatgtta tttatggcta aggttcaaaa gccatgggca ctatttatat
     1081 ttggtatgat atcaccactt gtgatgtttg cagctggtca tacctacgta gttgtggttt
     1141 tatcacttat agtaatgata atagcagaat taattagaaa gattggtaat tataattcat
     1201 ttaaatacaa tatgctttct tatgcaatct tcagcacatg gatatgtagc tctttaatgc
     1261 aaatgctttt agcaaaagaa aaatatatgg agtggtcttt gatgacgatg ggaaaagatt
     1321 atgttgatgt attagaaaag ttaataattt atcctcacat ggctttagta gccttaggtg
     1381 ctttcttagg aggaattctt ggagcatata taggcaaggc tctattgaaa aaacactttg
     1441 aaaaagcagg catcgtataa tgcctacttt ttcttcaaat gctcccttta atcccaatcc
     1501 gatctctaag atattagtcg tatttatcac aggcttaact gttatgcata gcataaacat
     1561 ccgttttgag ctggcaattg tttgtcttat tggtattttg ctttatttga atggatacaa
     1621 aaaaacactt ttcaaatgga ttatattgtg tgggatactt tactccttgc ctaattttat
     1681 ggtgttatct gaattaaacc ctatagttaa gatgtttttg agtataccta ttgttattag
     1741 aatgtttatt ttaccattta tggcagcaag ctttatgata aagacctcgg atgtaggcgc
     1801 aataatttca tcgatggata agcttaagat ttcaaagaat gtatccatac ctattgcggt
     1861 tatgtttaga ttcttcccat cttttaagga ggagaagaaa aacatcaaaa tggctatgag
     1921 agtaagaggg ataaatttta aaaacccagt caaatatctt gaatatgttt ctgtgccact
     1981 actcattata tcatctaata tatcagatga cattgcaaaa gcggcagaaa caaaggcaat
     2041 agaaaatcca attgccaaga ccagatacat tcgcgtaaag atacagctaa ttgattttgt
     2101 ttatgtttta gcggttgctg gacttattgt gggaggctta atatggttga aataaaaaat
     2161 ttaagtcttg attatggtga agagcatata ttagatgata tatcactatc catagccgag
     2221 ggagagtgcg tgctatttac aggaaaaagt ggaagtggta agtcatcttt aataaattca
     2281 atcaatggac tagctgtaag gtatgataac gcaaagacaa agggcgaaat aattattgat
     2341 ggtaagaata taaaaaattt ggaactttat caaatctcaa tgcttgtttc aactgttttt
     2401 caaaatccta agacatattt ttttaatgtc aatacgacat tagaattatt attttatttg
     2461 gaaaatatcg gtcttgcaag agaagagatg gacaggcgtt tgaaggatat gcttgagata
     2521 ttcccgataa aaaatctttt gaacagaaat atatttaatc tatccggcgg tgaaaaacaa
     2581 attctttgca ttgcagcttc ttatatagca ggtacaaaga ttatagttat ggatgagtct
     2641 tcatcgaatt tagatattaa aagcataagt gttttggcaa agatgctaaa gatattaaaa
     2701 gagaaaggca taagcataat tgttgcagag catagaattt attatttgat ggacatagtt
     2761 gaccg
//
LOCUS       DAVHIT010000129         2765 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVHIT010000129 DAVHIT010000000
VERSION     DAVHIT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.131"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            88..630
                     /locus_tag="VVN16_001970"
     CDS             88..630
                     /locus_tag="VVN16_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU5573274.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(671..744)
                     /locus_tag="VVN16_001971"
     tRNA            complement(671..744)
                     /locus_tag="VVN16_001971"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(708..710),aa:Asn,seq:gtt)
     gene            complement(750..821)
                     /locus_tag="VVN16_001972"
     tRNA            complement(750..821)
                     /locus_tag="VVN16_001972"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(786..788),aa:Glu,seq:ttc)
     gene            complement(873..1625)
                     /locus_tag="VVN16_001973"
     CDS             complement(873..1625)
                     /locus_tag="VVN16_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5573275.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1622..2563)
                     /locus_tag="VVN16_001974"
     CDS             complement(1622..2563)
                     /locus_tag="VVN16_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5573276.1"
                     /translation="MLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLLLHIVS
                     FVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQ
                     NYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLRSDK
                     YTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLL
                     NNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGV
                     GLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
BASE COUNT          951 a          510 c          402 g          902 t
ORIGIN      
        1 taatagatta tacattaaaa aatctaaatt acaagataaa aaatctattt ttagacatat
       61 agtctaattt gtttacaagg aggaaatatg caagaaagta acaaacgctt aaaaacaaag
      121 cgaactattg aaaatgctat ggtacaatta ctgatggaac agccatttga taaaatttct
      181 actgtcaaat tagtagaaaa agccggaatt agtcgttcca gcttctatac tcactataag
      241 gataagtatg atatgattga gcactatcaa agcaagctat tccatacatt tgaatatatt
      301 tttcaaaaac atgctcatca caaaagagac gctattttag aagtttttga atatctagag
      361 tcagaaccac ttctggctgc ccttctttct gaaaatggga ctaaagaaat ccaaaatttc
      421 ttacgaaata aacttcatat catgcttagt acagatttac aaaagcgatt tatgcaactg
      481 aatctaaata ccaccgaatt agaatatagt agcatctatc taactcatgc actttttggt
      541 gtctgccaaa cttggattgc acatggaaaa aaagaaagtt ctcaagaaat aacagacttc
      601 cttatgaaaa tgcttggtga tacaaattga tacaaaaaga ggaacacagc tgtgttccct
      661 tttatctata ctccgccagt aggactcgaa cctacgacat catgattaac agtcatgcgc
      721 tactaccaac tgagctatgg cggataaaat agtccgtacg ggattcgaac ccgtgttacc
      781 gccgtgaaaa ggcggtgtct taaccccttg accaacggac cttctatctg tagcagatat
      841 aaccattata tcaatttctt gctaattgtc aatcactttt gagatttttt ctctaaaata
      901 tcttttaatt ttctaatttt taatcttgaa ataggacaac gatggtcttc atagaaaaca
      961 atttctaagt tttttcgatc aatttctctg atattgccta tatttaccaa aaatgacttg
     1021 tgaggagaat aaaatcgctg agtatgtttg tccttttcct gaatatctgt catggtacca
     1081 taaaactctt ttgcaaaatt cttaccaata atgcgcaatt tatgagagac ccctgttgtt
     1141 tcaatataca aaatatcatg gtaaggaatt tttaaatcat ttcccttgta attgtagtcg
     1201 aaataatcta caacatcttc attttcaagt aacatactct tcgtgtagaa gatattttgc
     1261 tcaattctct tcttaaacat ctcatcattg atatccttat caacaaaatc tagggctgat
     1321 acctggtatt tataggttag agtcgcaaac tctgatcgac tagtgataaa gacgataata
     1381 gcgtaaggat tgtaatgacg aatgagctga gccacttcaa atcccttttt ctcaattcca
     1441 tgaatatcga tatctaggaa ataaagctga tttacttcat cattttcaat gtattcttca
     1501 aattcacgga cttttcccgt tgtcttgtat gatattggaa tattcgattc tttcgaaatt
     1561 tcatccaata ttctctctag tctcacttga tgttcaataa catcttctaa aattaaaact
     1621 ttcattcaaa ttccctctta aatctaatga tttgtctaaa tgtactgcct tccatctctg
     1681 tttctaaaat aatattgttg tacttatcta gtagttcttt cacattattt aatccgactc
     1741 cgcgatttct tcccttagtg gagaatccta aggcaaatag atctcctgaa ggagtcatcg
     1801 tcattttaca tgaattctga atcacaataa ctgtttcagt ttccatctta ataactgcta
     1861 cttccatctg ctttttatag ctatcagccg atccttcgac agcattattc aataaaacgc
     1921 tcatgatacg aaccaaatcc aatagttcaa ttggaagctt ggtaatcgta tcttttactt
     1981 ccagtgtaaa ctctacacca ttatttcgag catagacaat tgactgagca accaaacttc
     2041 gtaaagctga gtcttctatg ttgttcaaat caaagtaagt gtacttatct gaacgcaatt
     2101 tatgatttgc tttgactaaa acttcattgt aaattctgtc aatttcctgt aaattaccac
     2161 tgtcaattgc catctgcatg ctgacaagca ttccagcata atcatgtcga aaaccacgga
     2221 tttcattata cagaccaaca atttcatctg tgtaattctg taaatgtttc tgttcaaatt
     2281 tcttctgctt caaagcaatc tctttctcca tttgaacttt atgagaattc attgcaaaga
     2341 aggtcaaaag gagagagata aagacaatag atgacaaaat acttccaaaa ctattcaaat
     2401 gtttaatcgt acttaccata tctgaaacga aagatacaat atgtagcaat agtaaagcaa
     2461 aaaatacttt tttcaagaaa ggataaaggt agtcctcgtc aaaataggct agttccaaat
     2521 ggaaatagta aatgattttt aatgtaacaa aataggttaa caccgtcaca acgaaaaaga
     2581 atgggaaatg atattgtaaa acaaaattat ctcctgttat agaggagaaa gttacggaca
     2641 gaaagttatg agtgctctca tataaaagag atagtagtaa acttaggaat agtcctctat
     2701 ccctctcata ctgtttcatc catcgaaaat aggaatataa tcccaaagga aataaaaatc
     2761 tttca
//
LOCUS       DAVHIT010000130         2646 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVHIT010000130 DAVHIT010000000
VERSION     DAVHIT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.132"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            30..101
                     /locus_tag="VVN16_001975"
     tRNA            30..101
                     /locus_tag="VVN16_001975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62..64,aa:Gln,seq:ttg)
     gene            263..1465
                     /gene="rpsA"
                     /locus_tag="VVN16_001976"
     CDS             263..1465
                     /gene="rpsA"
                     /locus_tag="VVN16_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU5573277.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1551..2646)
                     /locus_tag="VVN16_001977"
                     /pseudo
     CDS             complement(1551..2646)
                     /locus_tag="VVN16_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          817 a          437 c          590 g          802 t
ORIGIN      
        1 ttcgaatccc tctctctcca tttcattaat ggggtatagc caagcggtaa ggcaagggac
       61 tttgactccc tcatgcgttg gttcgaatcc agctacccca gttcttaggt aataatcaag
      121 ataaaaagca aaatatctta gggtatttta tttttataat tgaaagacgt gaatgatatg
      181 aacatgtcct tgcgggtgct taggaaaaaa attataagta tgtcaagttt aagaaaaact
      241 tgattgttgg aggatttttt agatgaacga atttgaagat ttgctaaata gcgttagtca
      301 agttgagact ggtgatgttg ttagtgctga agtattgaca gttgatgcga ctcaagctaa
      361 cgttgcaatc tctggaactg gtgttgaagg tgtcttgact cttcgcgaat tgacaaacga
      421 tcgtgatgca gatatcaatg actttgttaa agtaggagaa gtattggatg ttcttgtact
      481 tcgtcaagta gttggtaaag atactgatac agttacatac cttgtatcta aaaaacgcct
      541 tgaagctcgc aaagcatggg acaaacttgt tggtcgcgaa gaagaagttg ttactgttaa
      601 aggaacgcgt gccgttaaag gtggactttc agtagaattt gaaggtgttc gtggatttat
      661 cccagcttca atgttggata ctcgtttcgt acgtaacgct gagcgttttg taggtcaaga
      721 atttgatact aaaatcaaag aagttaacgc taaagaaaac cgtttcatcc tttcacgtcg
      781 tgaagttgtt gaagcagcta ctgcagcagc tcgcgctgaa gtattcggta aattggctgt
      841 tggtgatgtt gtaactggta aagttgctcg tatcacaagc ttcggcgctt tcatcgacct
      901 tggtggtgtt gacggattgg ttcacttgac tgaattgtca catgaacgta acgtatcacc
      961 aaaatcagtt gtaactgttg gtgaagaaat tgaagtgaaa atccttgatc ttaacgaaga
     1021 agaaggacgt gtatcacttt cacttaaagc aacagtacca ggaccatggg atggcgttga
     1081 gcaaaaattg gctaaaggtg atgtagtaga aggaacagtt aaacgtttga ctgacttcgg
     1141 tgcatttgtt gaagtattgc caggtatcga tggacttgtt cacgtatcac aaatttcaca
     1201 caaacggatt gaaaatccaa aagaagctct taaagttggt caagaagttc aagttaaagt
     1261 tcttgaagtt aacgcagatg cagaacgcgt gtcactttct attaaagctc ttgaagaacg
     1321 tccagcccaa gaagaaggac aaaaagaaga aaaacgtgct gctcgtccac gtcgtccaag
     1381 acgtcaagaa aagcgtgatt tcgaacttcc agaaacacaa acaggatttt caatggctga
     1441 tttgtttggt gatatcgaac tttaatcaaa ttgaaaattc acaaaatcct ttgtttacta
     1501 aacaagggat ttttctggct ctttgtcaac tgtagtgggt tgaagaaaag ctaaggttga
     1561 gaaaggacaa atttcgtcct ttcttttttg atgttcagag cgataaaaat ccgttttttg
     1621 aagttttcaa agttccgaaa accaaaggca ttgcgcttga taagtttgat gagattattg
     1681 gtcgcttcca atttggcgtt tgaatagggt agttgaaggg cgttgacgat tttctctttg
     1741 tcctttagaa aggttttaaa gacagtctga aaaagaggat gaacctgctt aagattgtcc
     1801 tcaataagtc cgaaaaattt ctctggttcc ttattataga gctgatagtg gtgtttcaag
     1861 tcttctgaat agctcaaaag cttgtttaaa atctctttat tggttaagta catacgaaaa
     1921 gtaggacgat aaaatcgctt atcactcagt ttacggctat cctgttgaat gagtttccag
     1981 tagcgcttga tagccttgta ttcatgggat tttcgatgaa actgattcat gatttggaca
     2041 cgcacacgac tcatagcacg gctaagatgt tgtacaatat gaaagcgatc aagaacgatt
     2101 ttagcattcg ggagtgaaac agtctgggag actgtttcag cctgagccta gaaatttaaa
     2161 agcgaagctg tttagccaag tcatagtaag agctaaacat atccatagta atgattttga
     2221 cgcgacatcg gacggctcta tcatatttaa gaaagtgatc tcggatgaca gcttgtgttc
     2281 taccctcaag aacagtgatg atatcgagct tttcaaaatc ttgcgcaatg aagctcatct
     2341 ttccctttgt aaaagcatac tcatcccaag acataatctc aggaagacaa gaaaaatcat
     2401 gtttaaagtg aaaatcattg agcttacgaa taacagttga agttgagatg gaaagctgat
     2461 gggcaatatc agtcatagaa atcttttcaa ttaacttttg agcaatcttt tggttgataa
     2521 tacgaggaat ttggtgattt ttcttgacga tagaagtttc agcgaccatc attttagaac
     2581 agtgatagca cttgaatcga cgctttctaa ggagaattct agtaggcata ccagtcgttt
     2641 caagat
//
LOCUS       DAVHIT010000131         2602 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVHIT010000131 DAVHIT010000000
VERSION     DAVHIT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.133"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..1002
                     /locus_tag="VVN16_001978"
     CDS             <1..1002
                     /locus_tag="VVN16_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5573278.1"
                     /translation="ILLNWLAQYRKNGYTIVEKTKGRVPESGECHPKKVKRTPIEGGK
                     RERRKTEIVQELMTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIE
                     HKGNYAYRRIYLELRNRAYLVNHKRVQGLMKVLNLQAKMRQKRKYSSHKGDVGKKAEN
                     LIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSCSPNLEQV
                     QTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMES
                     FFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG"
     gene            complement(1118..1921)
                     /locus_tag="VVN16_001979"
     CDS             complement(1118..1921)
                     /locus_tag="VVN16_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU5573279.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          894 a          465 c          453 g          790 t
ORIGIN      
        1 atacttctta actggctagc acaatacagg aaaaacgggt atactattgt tgagaaaaca
       61 aaagggagag tacctgagag cggagaatgc catcctaaaa aagttaagag aactccgatt
      121 gaaggaggaa aaagagaaag aagaaagaca gaaattgttc aagaattaat gactgagttt
      181 tcgttagttc ttcttctaaa atccattaaa ctagctcgtt ggacctacta ctatcacttg
      241 aaacagctag acaaaacaga taaggaccaa gagcttaaag ctgaaattca atccattttt
      301 atcgaacaca agggaaatta tgcttatcgt cggatttatt tagaactaag aaatcgtgct
      361 tatctggtaa atcataaaag agttcaaggc ttgatgaaag tactcaattt acaagctaaa
      421 atgcgacaga aacgaaaata ttcttctcat aaaggagacg ttggcaagaa ggcagagaat
      481 ctcattcaag gccaatttga aggctctaaa acaatggaaa agtgctatac agatgtgaca
      541 gaatttgcca ttccagcaag tactcaaaag ctttacttat caccagtttt agatggcttt
      601 aacagcgaaa ttattgcttt taatctttct tgttcgccta atttagaaca agtacaaaca
      661 atgttggaac aggcattcac agagaagcac tacgagaata cgattctcca tagtgaccaa
      721 ggctggcaat accaacacga ttcttatcat cagtttcttg agggtaaggg aattcaagca
      781 tccatgtcac gtaagggaaa cagccaagac aacggtatga tggagtcctt ctttgggatt
      841 ctgaaatcgg aaatgtttta tggctatgag aaaacattta aatcacttaa tcaattggaa
      901 caagctattg tagactatat tgattactac aacaataaac gaattaaggt aaaactaaaa
      961 ggacttagcc ttgtgcaata cagaactaaa tccttcggat aaattatttg tctaactttt
     1021 tggggtcagt acacctaaaa ctttgatgat atacgttttc ttgtgagaat atttacttca
     1081 tttttgccta aaattcaatg tttactcagt atttggatta tgaaaaatcg aggtctaaat
     1141 ctagatacat tttttctgaa gacaaatcat tttgaccacc gagcaagaga ttttcaaaaa
     1201 aagctgttaa aaactcagaa cgtcgctgta aaatctttgc attatctaat accaaggcat
     1261 cacgaaaata tttggaatgt tgctgaaatg gtgtattatc aatatcaaaa ccaaactcac
     1321 gaagatactg aatcaaaaag accgttactg tccgagtgtt tccttcgcga aatggatgaa
     1381 tctgccagat tcctgaaata aaatgctgga tttgtttaac cacatccgcc tgagttagtg
     1441 tcgcatatgc aacttgtttt tcctgattaa aatcataatc taaggtcatt tgaatcatgg
     1501 agtaatcaga gtacacaaca ctttcaccat tcaaaacagg ttcattcttt gtgatattgg
     1561 tctgacgaaa ttgccccacc ggaatagagg gttcaaatat atcttgaaac aactccttat
     1621 gaatagcaag taaggtcgca ggactaaagc taaagcctct tcgagacaat agttctacaa
     1681 tacgtagaga aaccaagtct gcctcttccg tacttgcatc aatagtatgg tgataagccg
     1741 ttgcatcctc ataaacctgc tcataagtca gttctccccg ggactgtttc tcagccaaag
     1801 attccatata cgctgatggc actagattgt caactttctg cagaccaaaa cctatccgcc
     1861 ataaatcacg cttcgcttca taagacaagt ttggattgtc aatgttgtaa gttgattgca
     1921 tagaaatatc ctttcaattg tttttctgtg tcatcggtaa attatgaaat aggtatggaa
     1981 ttggcgaaaa ttataaaaat ccttcacaat aggttctatg aaatatatat acttcatctt
     2041 aatagtgagc aagctaaact tagcatttca tgccctcata tgggatgttc tttaactaaa
     2101 taatatgatt atcgagatat atctggataa atgaactaat aagtctgacg cgtagactta
     2161 tcaaagtcat tggcatacac cactatgaac tcgttgatct gttcaaatcc caacacatta
     2221 cctgagaaga aagttgcaat gttgtttttg gtgcgggttt gaatttaaaa atttgttatg
     2281 tagtacctaa tctaaggaat tagaacaatg cctctaattt ttctttaata cactgaaaca
     2341 ttgatgattc tggctgtatt tttgaaacag ctcttctttg ctcctggaaa atatcttcag
     2401 aagttatatt ctctattcct aacgctactt gaattttttt ctaaaatatt cttttccgtt
     2461 gccatcttta gaaaaatcat aaccttccct atctacgctg ttacataaat tagctaaaaa
     2521 aaactatggg gttgggaaag gaagataaga aacgtattta gcccaataat ctataaagct
     2581 tctttgtttc gcaattaatt ca
//
LOCUS       DAVHIT010000132         2547 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVHIT010000132 DAVHIT010000000
VERSION     DAVHIT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.134"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            11..>2547
                     /locus_tag="VVN16_001980"
     CDS             11..>2547
                     /locus_tag="VVN16_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5573280.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKLAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDLDK
                     GYSGFRAAPAPTNKLVTNLGNNTVFTDISRGSHTFRGNGNSRGGNPIHFDVTTTRVGN
                     RVEFSIAYSGPGEFVNNNFLLDKGNGFGEPSRATISTNRLRDQTKDIRKGANFVSHSS
                     YSLTSAVETNSNQTIRFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDGAKGEKGVQGERGLTGAQGQAGRDGVTPTVTVKD
                     NKNDGTHTITINDGRGNVASTVVRDGFDGASPLVATQRNEADKTTTVIFYYDQNGNNE
                     LDASDKKLKEVIIADGAKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPG
                     KDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSV
                     QNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHT"
BASE COUNT          919 a          457 c          536 g          635 t
ORIGIN      
        1 ggagtttaaa atgaaacatt cacataaaaa atcatttgac tggtatagca tgcaacaacg
       61 ttattctatt cgtaagtatt actttggtgc agttagtgtc ttgctcggta ccgctttggt
      121 attaggtgca gcagctagtg tccaaacagt acaagcggaa gaaaacaaac aagaaactac
      181 caatagtatt tctgttggta ggggagaagc agctactaaa ctagcagagg tttctgcgtc
      241 taataaagag aaaacctatg cagctccaac tgtagctaat ccagtagaaa cgactccagt
      301 taaaactgga gaagttacta aaccagcaga aaaagttgaa gaagcaaaag acaaaaaaga
      361 ggaagtaacg catcaagatg ctattgacaa gtcaaaattg ttaacggctc tttcgcgtgc
      421 taaaaagtta gaaagcaagt tatatacaga agcaagtgct gctaacttgc aaacaagtat
      481 ccaagctggt caaagcttgc ttgggaaagc agatgcatct gaagctgaat tatcagcagc
      541 agagtcatct attcaatcat ctattattgg tctagaactt cgttctaact ctaacaaagg
      601 aactgtatca gaaactcctg tagcgaagaa agctaatata gttgaagcga aggaagagac
      661 taatccagct gcaacaactg atcgttcagc tgttgatagt gctgttttgc caactagcac
      721 agctgccaaa gtagaaacaa cttcagctcc agcatctatt aatgaaatct tgaaaccagg
      781 tttgagcctt tctgatgctc gccaaaatcc agctatccgt aaagaagact tggataaggg
      841 gtatagtggt tttagagcag cacctgctcc aacgaacaaa ctagtaacta atctaggaaa
      901 taatactgtt ttcactgata ttagcagagg gtctcatact ttccgtggta atgggaattc
      961 acgtggtgga aacccgattc attttgatgt aacaacaact agagttggaa atagagtaga
     1021 attctcaatt gcatattctg gtccaggtga gttcgttaat aataactttc tcttagacaa
     1081 agggaatgga tttggagaac catctcgtgc aactatttca acaaacagat taagagatca
     1141 aactaaagat ataagaaagg gtgcaaattt tgtatctcat tcaagctaca gtttaacatc
     1201 tgctgttgaa acaaacagta atcaaactat tagatttagt ttacctatag ccaaccctaa
     1261 tggtgatctg tctgttcgat taagacctgt tacttttaat gtaggtcaag gggaatcagg
     1321 atctgcaact agtaatgatc catatagtaa ctctaactat tacttcagag aaaaaccact
     1381 cctcttggat gcaaatccaa atggtggtac taataataag actgtttcag aagacattga
     1441 cttccaaact gtctatcttc caactagtaa gttaccagaa ggtcaaacta gattagttcg
     1501 agaaggtgaa aaaggacaac gtcaaatcat ctataaagtt catcaattgg gtaacgaaac
     1561 aatcctagga ttgccaatta gtaattcagt tattaaagaa gttaagccac gtattatgca
     1621 aattggtgtg gctaaagatc taatcgatac agtaaaacca cgtgttgatc aaaataaagt
     1681 cggtgataca aataacctca ctttctatct tgataacgat ggaaacggtg tttatactga
     1741 gggcgtagac gaacttgttc aaaaaattgc tattaaagat ggtgctaaag gtgaaaaagg
     1801 agtccaaggc gaacgcggcc tgactggagc gcaaggtcag gcaggtcgtg acggtgtaac
     1861 tccaaccgta accgttaaag ataataaaaa tgacggcact catactatca ctattaacga
     1921 cggtagaggt aatgttgcaa gtactgttgt aagagatggt ttcgatggcg caagtccatt
     1981 agttgcgact caacgaaatg aagcagataa gacaacaact gttatcttct attatgatca
     2041 aaatggaaat aatgaattag atgcttctga taagaaatta aaagaagtta ttattgcaga
     2101 tggtgccaaa ggtgaacaag gtcttcaagg tcgtgatgga gctcaaggac caaaaggcgc
     2161 agatggacaa agaggaccag cgggaccaca aggaccaaaa ggagaacaag gtaatccagg
     2221 aactccaggt aaagatggaa aatctctaat tgctgttaaa aatggaactg aaacaaaagt
     2281 ctatgtagag gatccggcta gaccaggaca accacttaat ccaaaccaac cattagcaac
     2341 tatcactgat ggaagaaatg gtactaatgg acaatcacca acaattacag cgacacgaag
     2401 tgttcaaaat ggtaaaaatg gtgtattagt aacaattaca ccagtaggag gtcgtccaca
     2461 aacaacattt gtagaggatg gacaaaaagg tgctgatggg aaaaccccaa cagtaacaat
     2521 aactgagggg caaaacggca cacatac
//
LOCUS       DAVHIT010000133         2408 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVHIT010000133 DAVHIT010000000
VERSION     DAVHIT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.135"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            43..327
                     /locus_tag="VVN16_001981"
                     /pseudo
     CDS             43..327
                     /locus_tag="VVN16_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1065..1325)
                     /locus_tag="VVN16_001982"
     CDS             complement(1065..1325)
                     /locus_tag="VVN16_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573281.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELELVKSTILQNDDISLDQNEIFEILKNFFDG"
     gene            complement(1384..1935)
                     /locus_tag="VVN16_001983"
     CDS             complement(1384..1935)
                     /locus_tag="VVN16_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573282.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNLEFPSMIELFSIL
                     PEYPLKKDGREISLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSTI
                     SSNHVSFHLQPQNSVLSSLRLAS"
     gene            complement(1925..2122)
                     /locus_tag="VVN16_001984"
     CDS             complement(1925..2122)
                     /locus_tag="VVN16_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573283.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(2126..>2408)
                     /locus_tag="VVN16_001985"
     CDS             complement(2126..>2408)
                     /locus_tag="VVN16_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573284.1"
                     /translation="WLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQI
                     KSVSNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQYVLK"
BASE COUNT          819 a          448 c          385 g          756 t
ORIGIN      
        1 ttgaagagat attagctgta ttaaaaacag cttatcaaaa aagtcggaca agctggtaaa
       61 atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc acaaactcca
      121 cgtaaatcca gcaaactcaa gccgctaaca gttgaagata aaacctataa ccatgcgcta
      181 tctaaggaga gaagcaaggt tgagaacatc tttgccaaag tagaaacgtt taaaatgttt
      241 tcaacaacct atcgaaatca tcgtaaacgc tttggattac gaatgaattt gattgctggt
      301 attatcaatc atgaactagg attctagttt tgcaggaagt ctattatata tgcacagaca
      361 tatgaaatct ttttgagata gcagttatga tatatttatc cattcacaac atcttctgta
      421 ccaatactac aatattggta cattttgatt acacaatcgt ttcaaaaaat atgatggaag
      481 cctgagtatt ttatagtaac tcccttccta tcaactagat caaccagttg agtgttgaat
      541 gttgaaacac cctagcctca gcaaaggact atgagcagca tttcctagcc aataggacgc
      601 tccaaatcac ctattctaaa tccatcacta gcttggtcag taagatacag actgcaaggc
      661 aagactgacg tggttgatga gattttggaa gagtattaca atcatagatt caccagtaga
      721 cttgaccgca gatttaacta tagtagattg aaactagaat agtacacttc tgcttctaaa
      781 acattgttag aaatcgattt gactgtcctg atcaatttgt cctgttctcg tttcatttta
      841 ctatactaaa aaaccacctg aaacaaatca agtgactaga ggataaaaac aaaaactggg
      901 caaaaagttt tgaaaagcaa aaaacgcata atatcaggtc ttgaaaaacc ttgatactat
      961 acgtttgatt gtgggaagat tgacttcatt ttctcctgaa attgaagttt tttctagttt
     1021 ccagatatag acgaattcaa tatagccatc tcgctacaat tattttaacc atcgaaaaaa
     1081 tttttcagaa tttcaaaaat ttcattttga tctaatgaaa tgtcatcatt ttgaagaata
     1141 gttgacttca caagctctaa ttctatcaca aagcggtgca tgtctgcttt taccgcacta
     1201 cttgatgtta tagccgataa cttttcatct cctagcaagg aagctgtcaa acgggtaagg
     1261 tcattcaaat gctttttaat ggacttactt aaaccttgag cacctgtagc agagcgttcc
     1321 ctcatatcca gccaagcttt agccttaaaa ataattaata ctcaatgaaa atcaaagagc
     1381 aaactaggaa gctagccgca ggctgctcaa aacactgttt tgaggttgta gatggaagct
     1441 gacgtggttt gaagagattg tcgaagagta tagaccacaa ttactcaata ccgaataccc
     1501 ctgaatggtt tcttttacat gcaccaatat attataataa tcttcatcca ataataaggc
     1561 tgataaacta gcatcttggt caaaatataa ggaaatttct cgaccgtcct tctttaatgg
     1621 atattctggt aagatactaa atagttcaat catagaagga aactcaagat tagttgttgt
     1681 aaatcgaaaa agctgcgctt tctcatcttt ctgacttcct tgatactctc ccaattctaa
     1741 aaaatgattc aaggtagtat aaaattcctt attttttact tcatcaatga tgaccatatc
     1801 ataatctttt gttgtgcgac ttttaaatcc ttgcgaatcc aatacgatag aggtagcagt
     1861 tcccccaatc agaacataat agttctgaaa atccgcaaac atttcttgaa aaataacttt
     1921 cgtattagct ggcatcatct tctcccagat actgtaatat catattttct agtgcttcac
     1981 tctcttcctc tatacgtggg tcatcatcat cttttaaggt caaataaaga gaaatcggat
     2041 ctacaaattg tttatcatga ttatttttaa aatcattcca aaactcagat acaaaaggac
     2101 gatatttcca tatctctagc atctttcatt ttaaaacata ctgagaaagt ggcaaggata
     2161 actgattgaa ttttctctgc catatgacat agctagtatt ttcatccgtt tcagctaaaa
     2221 aagttgaatg cgacaaagca taagcaccac catatagaag gttagaaaca gattttatct
     2281 gctttatatc gccatctggc aataaaatcc gttttttgat gggattaaat aaacatgaca
     2341 cggattttaa gaataattct ttctttgaca ccgtatatgt gtaaagctta ttttgcttgt
     2401 ttaaccaa
//
LOCUS       DAVHIT010000134         2398 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVHIT010000134 DAVHIT010000000
VERSION     DAVHIT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.136"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..1296)
                     /locus_tag="VVN16_001986"
     CDS             complement(<1..1296)
                     /locus_tag="VVN16_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573285.1"
                     /translation="MNTKIKNIITSFSYVISSNLLIVLTSSLVVLIFPKIMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYTNLDRKQFYSQMILFSSFLMLISLVLF
                     TLNLITVRDENARYIYNMAIISMIVTNLRTLYVYILQMTNRLKDSSVILISDRVLYVL
                     LLFMFIVFGWHEYKVMIWADILGRTFSLMLSFWICKDIVFQSLSKFILDFKESLDNIR
                     VGINLMLSNIASSMIIGIVRMGIQWNWNIETFGKVSLTLSISNLLMTFINAIGLVIFP
                     LIKRTKTENLPKIYSNLRNALMLVMFAILLFYYPLKFILDIWLPAYKDALVFMALIFP
                     MSVYEGKMALVINTYLKAMRMEKDILKINALVMLTSMVVTLVTTLLLNNLGLTVVSIV
                     ILLALRSIIAELILSKKLKISVKQDIALELLMTIIFISSS"
     gene            complement(1319..>2398)
                     /locus_tag="VVN16_001987"
     CDS             complement(1319..>2398)
                     /locus_tag="VVN16_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU5573286.1"
                     /translation="KIADRLIDIQFWKIALTIIIDLFILYLYRKEIYNLVLSNGYTGS
                     NIQWFFRNATSYEGELTVRTFIRVLIRVIDVSAYIFGYTFINNFLIYRHKRPKDILLL
                     VPLLIFISKTLISGGRQDIIKILIAYVIMMYIQQKRKVGWNRVISHKYIHLGFVGLIA
                     GIPAFYYSLFLAGRSTTRTLFESVSTYLGGSIQHFNQYIENPLDPGEVFGSETLVPIL
                     NILGEMGLVNYRSTIHLEFRTLGVTVGNVYTFFRRPLHDFGLVGMYVFVFAVGAFFAI
                     YYLVLRKKQVGFNLDIHTIIYSYVFYWIFLSSIEQYSFTMISLYTLVFIVLVYFMAIF
                     YWCTDFKRGKLIFKISDSSIKLKEE"
BASE COUNT          942 a          415 c          320 g          721 t
ORIGIN      
        1 acttgaagaa ataaatataa tcgtcataag taactctaaa gcaatgtctt gcttgactga
       61 tatcttcagt tttttggata aaattaattc agctattata cttcttaaag caagtaaaat
      121 aactatagat acaactgtca gccccaaatt atttagtagt agagtagtca ctaatgtcac
      181 taccatacta gttaacataa ccaaagcatt aattttgaga atgtcttttt ccattctcat
      241 tgcttttaaa tatgtattta tcaccaaagc cattttccct tcataaactg acataggaaa
      301 aattagggcc atgaaaacta aggcatcctt ataagcagga agccaaatat caagaataaa
      361 ttttaaagga tagtagaaga gcaagattgc gaacataacc aacatcaaag catttcttaa
      421 attagaataa attttaggta aattctcagt ctttgttcgc tttatcaaag gaaagataac
      481 taatccaatg gcattaataa aagtcattaa taaattagat atactcaaag ttaatgacac
      541 tttcccgaat gtttcaatat tccaattcca ttgaattccc atacgaacaa tgcctataat
      601 catgctactc gcaatgttag atagcattaa attgattcca acacggatat tatcaaggga
      661 ctccttgaaa tctaagataa attttgacaa agactgaaac acaatatctt tacaaatcca
      721 gaaggaaagc atgagagaaa atgttcgacc tagaatatca gcccaaatca tgaccttgta
      781 ctcatgccat ccaaatacaa taaacatgaa taaaaggagt acatataaaa cgcgatcact
      841 aattagaatg actgaactat ccttcaagcg atttgtcatc tgcaagatat aaacatagag
      901 tgttcttaag tttgtgacta tcatgctaat gatagccata ttataaatat atcttgcgtt
      961 ttcatccctt acagttatta ggttcaaagt aaataatacc agcgagatta gcattaagaa
     1021 actagaaaat agaatcatct gagaataaaa ctgttttcta tctaaatttg tatattctaa
     1081 gccaccatat ttgagataaa tcccatcaat ccaacccaag tggaaaaaac cgatataggt
     1141 cagataaaaa atataaagtt gccagtaact gtactcagtt acccccatta ttttaggaaa
     1201 aatcaaaaca actagtgatg aggttaaaac tatgagcaga tttgaagaaa taacataaga
     1261 aaaactagtt attatatttt taattttagt attcatctac cctctcctat acattctgtt
     1321 attcttcttt taatttgata cttgagtcag aaattttaaa aatcagtttt cctcttttaa
     1381 aatctgtaca ccagtaaaag atagccataa agtaaaccaa cacaataaat acaagtgtat
     1441 atagactaat cattgtgaac gagtattgct cgattgatga taaaaaaatc caataaaaga
     1501 cataagaata aataatggta tgaatatcca aattaaaacc aacctgtttc tttctcagaa
     1561 ctaaataata aatagcaaaa aaagcaccta cagcaaagac aaacacatac ataccaacta
     1621 gaccaaaatc atgcaagggt cttctaaaaa aagtataaac atttcctaca gtaactccta
     1681 gtgtccgaaa ttctaaatga attgtactac gataattaac taggcccatt tcccctaata
     1741 tatttaatat aggcaccaat gtttcactgc caaaaacttc accaggatct aatggatttt
     1801 caatatactg attaaaatgc tgaattgagc ctcctagata ggtcgaaaca ctctcaaata
     1861 gcgtcctagt cgttgaacga ccggctaaaa acaaagagta gtaaaatgct ggaatacctg
     1921 ctattaaacc aacaaatcca aggtgaatat atttatgaga tatgactcta ttccatccaa
     1981 ctttccgttt ttgttggata tacatcatga ttacataggc aatcagaatt ttaataatat
     2041 cttgccggcc tcctgatatt aaagtttttg aaataaatat taataaaggt actaaaagta
     2101 atatgtcttt agggcgttta tggcgataga taagaaaatt attaataaaa gtatatccaa
     2161 aaatataagc agatacgtca ataacacgaa tgagaactcg aataaaagtt cgcactgtca
     2221 attcaccttc ataactcgtt gcatttctaa aaaaccactg aatatttgac cccgtatatc
     2281 cattactaag aacaaggttg tatatttcct tcctgtaaag atacaaaata aagagatcaa
     2341 ttataatagt tagagcaatt ttccaaaatt ggatatcaat taatcgatca gcaatctt
//
LOCUS       DAVHIT010000135         2209 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVHIT010000135 DAVHIT010000000
VERSION     DAVHIT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.137"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..145
                     /locus_tag="VVN16_001988"
     CDS             <1..145
                     /locus_tag="VVN16_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5573287.1"
                     /translation="CDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKGERNITN
                     GFK"
     gene            132..338
                     /locus_tag="VVN16_001989"
     CDS             132..338
                     /locus_tag="VVN16_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573288.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            384..743
                     /locus_tag="VVN16_001990"
     CDS             384..743
                     /locus_tag="VVN16_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573289.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLG"
     gene            872..1297
                     /locus_tag="VVN16_001991"
     CDS             872..1297
                     /locus_tag="VVN16_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573290.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKV"
     gene            1705..>2209
                     /locus_tag="VVN16_001992"
     CDS             1705..>2209
                     /locus_tag="VVN16_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573291.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFK"
BASE COUNT          790 a          265 c          466 g          688 t
ORIGIN      
        1 atgtgatgtt ggaaaaagaa taaaagaact tagaatatct tcaaatctta ctcaagataa
       61 gattgctgag tatttgtctt tgaatcaaag catgattgcc aaaatggaaa aaggtgaaag
      121 gaatatcacg aatggattta agtaataaag ctccaaatct tagaaaaaag ttgggagctg
      181 atggtgaatc gccgatagat atttttaaat tggtacaaaa gatagaaaat ttgacgctgg
      241 tattttatgg actcggaaag aatattagcg gagtctgtta taaaggaact cagttcagtc
      301 tcattgcagt caattcagat atgccattag gaaggtaaag attttcttta gcacatggac
      361 tgtatcatct ttattatgat gaggtgaaga agagttcagt cagtcttatc ttgattggtg
      421 aaggagatga aactgaaaga aaagcggatc agtttgcttc ttatttttta attttcccat
      481 cttcactgta taggatggtt gaggaaatca gagaaaatgc caatagaact catcttgaag
      541 tagaagatat tataaaattg ggtcagtttt atggtatcag tcataaagtt atgttatata
      601 gattgaggaa tgatggatac cttgatgcag aagaaattaa aaatatggat attagtgtta
      661 tagagacagc ttcaagatta ggctatgata caagtttata tcgtcctttg tcagaaagta
      721 aaaaggaaat ggtattagga taatatatta attcaactga acaactttta gaaaataaca
      781 gaatttcgca agggaagtat gaggaactgt tactagatgc tttcagatat gatattgtat
      841 atgggctaga tgaagagggg ggagttgtcg tttgactagt cgtgtattta ttgatgcaga
      901 ttgtatttca gtatttttat gggttggcac tgaacatctt ttagaaaagc tctattcggg
      961 taaaattgtt attccacaag aggtgtatga tgaaatcaat atacctacaa ttccccattt
     1021 aaaatctagg atagaccagt tggtagctaa gggttcagct gagattgtga gcatagacat
     1081 tggaactgaa gaatacgcat tatatagaga tttaacaaga aatcatgata gtaacaagat
     1141 tattggtaag ggagaagcgg cttctatttc cttagcgaaa aagcataatg ggatattagg
     1201 aagtaataac ctaagagatg ttaaatcata tgtagaagaa ttttctttag aacatatgac
     1261 aacaggagat atactgattg aagcgtttaa agtgtaattt attactgaac aagagggcaa
     1321 tcatatctgg aataatatgc ttaaaaagag aaggaaaatt gttgcaaatt cattttcaga
     1381 ctatcttcgt ggaagtattc atcaaaatag acaaaaataa atttggataa atcgaactca
     1441 ctattcagga ggcatatgag caattcgaaa aagaaaagtg tcaaattgag cctataggag
     1501 tagaagtgaa atagtaagtc ctgcatagtg gatgagagaa aagttctcct tgaagttttc
     1561 ctgaactatc agtcgcatgt caaacgatat gtagggtaat gtgagagggg atagcgagta
     1621 gtttttggtt attttatcaa aaaacttata ttttattata ctgaatgata aaatataata
     1681 aaaatgatag aataaggaaa aaacatgaat gtcaaaaaga taatgtcaat ttttcaatcc
     1741 ttttatgttg atgtcagtat tgaggaactg actttgactt taccaatcag ttttgtaaaa
     1801 aggtttgagt atactcaaat gacttttcat aaggaatcat ttttattgat taaagaaaag
     1861 agaaggggga gtttgagttc atttgttact caggctcgca ctatgggtga aaaagccaat
     1921 atggatgttg ttttggtgtt ttcgaagtta tcagacagtg aaaaaaagca attacttcaa
     1981 gctagagttc cgtttgtaga ctttaaggga aacctcttct tccctccatt gggactagta
     2041 ctgaatgcga atgatactga agtccctaag gaattaacac ctagcgaaca attaacgtgg
     2101 attgcctttt tattgacaaa aggtcaaaaa gtagtagatg ttgatttgct ttcacaagtc
     2161 actggacttc caaactcaac aatttatagg tgtttgagga cttttaaag
//
LOCUS       DAVHIT010000136         2201 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVHIT010000136 DAVHIT010000000
VERSION     DAVHIT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.138"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            78..1601
                     /locus_tag="VVN16_001993"
     CDS             78..1601
                     /locus_tag="VVN16_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573292.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGITLLALTIIGGLILGAMMGE
                     QEFMKIYQEALSKLSAETVEYVRGMQVVKIFKANVESFKIFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHSNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1567..>2201
                     /locus_tag="VVN16_001994"
     CDS             1567..>2201
                     /locus_tag="VVN16_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573293.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMPAG
                     NVKMGLAVIIPSILNFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQ"
BASE COUNT          776 a          305 c          414 g          706 t
ORIGIN      
        1 tatccggtgc agagagcata gcattaaaat ctgttattac actaacaagt ggagcgatat
       61 tttattttgt ctcaggaatg ttttcacata tcttgggatt caggcttgaa acaaatttaa
      121 gaaaaagggg aatcgatggt ctggaaaaag caagttttag gttctttgac ttaaatccat
      181 ctggtcaaat aagaaagatc atagatgata atgcagcaca aactcatcag gtggtagctc
      241 acatgattcc cgatagctct caggcaatag tcacacctat acttgtcctt gtactaggct
      301 ttatagtaag tataagggtt ggtataactt tgcttgctct tactataatt ggtggcttaa
      361 ttttaggggc gatgatgggc gagcaagaat ttatgaagat ataccaagaa gctctatcta
      421 aactaagtgc tgaaactgtt gagtacgtga gaggaatgca agttgtaaaa atatttaaag
      481 caaatgtaga gtcttttaaa atcttttata aggcgataaa agattactca aagtatgctt
      541 atgattattc cctatcttgt aaaaggcctt atgttttgta tcaatggtta ttttttggac
      601 tgattgcaat tttaattatt cctatagttt attttatgac tagcttagct agcgcaaagg
      661 tgattttact tgagcttatc atgattttat ttttatcagg agttctcttt gtttcattca
      721 tgagaatgat gtggtactcc atgtatattt ctcaaggaaa ttatgcagta gatactttag
      781 aggcgcttta cgaagatatg caaaaagaca aattagtgca tagtaatgtc aataatttta
      841 aaaactataa tatagaattt gagaatgtta gctttgctta taatgataaa gctgtcattg
      901 aaaatttatc ctttaattta gaagaaggaa agtcctacgc acttgtcggt tcatctggat
      961 caggcaaatc aacagtagca aaacttatat caggttttta caatgttaat aaaggaagca
     1021 taaagatagg cgggatagca ataagtgaat attctgacga agccttaatt aaagccattt
     1081 cctttgtttt tcaagattca aaattattca agaagagcat ttatgataat gtagctttag
     1141 ctaataaaga tgcgacgaaa gatgacgtta tgagagcctt aaaattagca ggatgcgatt
     1201 taatattaga caaattccca gaaagagaaa atacaatcat aggctcaaaa ggtgtttatt
     1261 tatccggtgg agaaaaacaa agaattgcaa ttgctagagc aattttaaag gattccaaaa
     1321 ttattattat ggatgaagca tcagcatcta ttgacccaga taacgagttt gaattgcaaa
     1381 aagcttttaa aaatcttatg aaggataaaa cagttatcat gattgcacac aggctatcta
     1441 caattaaaga ccttgatgaa attattgtca tggatagtgg aaaaattata gaaagagggt
     1501 ctgacaaaga attaatgtca aaagatacaa ggtataagag cctgcaagag atgtttaaca
     1561 gtgcgaatga atggagggtt tcaaatgaaa gagttttata aaaaaagatt tgctcttaca
     1621 gatggaggag caagaaattt aagtaaagca acactggctt catttttcgt ttattgtata
     1681 aacatgcttc ctgccatatt acttatgatt tttgctcagg aagttttgga aaatatgggc
     1741 aaaagcaatg gcttttatat agtattctca gttttgactt tgatagcaat gtatattttg
     1801 ctttctatcg aatacgataa attatataac acaacctatc aagaaagtgc agatttaaga
     1861 ataaggacag cggagaattt atcaaaatta cctctatctt acttttctaa acatgacatt
     1921 tccgacattt cacaaacaat catggctgat attgaaggca tagagcatgc aatgagccac
     1981 tcaataccaa aggtgggcgg catggtactg tttttcccat taatatctgt aatgatgcca
     2041 gcgggcaatg tcaagatggg tttagctgta attattccat ctattttaaa ctttatattt
     2101 atacctttat ctaaaaaata tcaggttaat ggacagaata gatattatga tgtcttaaga
     2161 aaaaactcag aaagctttca agaaaatatc gaaatgcaaa t
//
LOCUS       DAVHIT010000137         2158 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVHIT010000137 DAVHIT010000000
VERSION     DAVHIT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.139"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            107..1114
                     /locus_tag="VVN16_001995"
     CDS             107..1114
                     /locus_tag="VVN16_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5573294.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLSVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1117..1317
                     /locus_tag="VVN16_001996"
     CDS             1117..1317
                     /locus_tag="VVN16_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU5573295.1"
                     /translation="MSEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1469..1651
                     /gene="rpmF"
                     /locus_tag="VVN16_001997"
     CDS             1469..1651
                     /gene="rpmF"
                     /locus_tag="VVN16_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5573296.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1667..1816
                     /gene="rpmG"
                     /locus_tag="VVN16_001998"
     CDS             1667..1816
                     /gene="rpmG"
                     /locus_tag="VVN16_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5573297.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
BASE COUNT          717 a          412 c          407 g          622 t
ORIGIN      
        1 aagaaattat tgggctgaaa gtttttagtg attattttca actttctatt gacttttaaa
       61 aacaaagtga tatcatttag atatcaaata atgggaggtc attcttatga ctgaaaaact
      121 aatcaattca aaaccaaatg gtgtattcgc attgattctc attgagttga caatcgtact
      181 tagtgtcttt atatttataa tgggtgttgg ttcggaaaac atttttggaa ttattatcgg
      241 acctttacta atcgtaattg caggtctagc tcatgctggt ttaaaagttg tcaaacctca
      301 ggaagctctg gttctgacac tctttggtaa ttatacaggt accatcaaag aacctggttt
      361 ttactttgtc aatcccttca gcgtagcagt caaccctgca aaccacactc gacttggaca
      421 aagtggtgat gttagcacaa aatctccttt tttaggagct aaatcatcaa atgacaatga
      481 tgtaaatctt gaaattggca agaaacacat ttccctcaaa gtcatgacct tgagcaattc
      541 tcgtcaaaaa atcaatgatt gcttaggaaa ccctgtagaa atcggtatcg cggtaacttg
      601 gagagttgtc gataccgcta aggcagtctt caacgttgat aactacaaag aatatctttc
      661 attgcaatgt gatagcgccc tccgtaatat tgtccgcatc tatccttacg atgtgtctcc
      721 taatgtggat actacgggtg acgggcaagc agatgaaggt agtctccgtg gctctagcga
      781 aattgttgct aaccgtattc gtgaggaaat tcaaagtcgc gtagaagatg ctggcttgga
      841 aatccttgaa gcacgtatca cttacctagc ttatgctcca gaaattgctg ccgttatgct
      901 tcaacgccaa caagcatctg ccattattga tgcacggaag atgattgtag atggtgctgt
      961 aggaatggtt gaaatggcac tagaacggct caatgaaggg gaattagtag aacttgacga
     1021 agaacgaaaa gctgccatgg tttcaaatct ccttgtcgtt ctttgtggca atcatgatgc
     1081 acaaccaatt gtcaacacag gaagtcttta ctaaagatgt ctgaagctaa aaaaaagcag
     1141 atcccccttc gactctcaac aaagttatac gctgcactcg catcatgggc agaagacgac
     1201 tttcgttcag tcaatgggca aatcgaatat ctcctcacag agtgtgtcaa gcaacggaag
     1261 aaagacggaa aatacgtatc agaaaccatt gacgaaccat ttgagattga tatttaacac
     1321 cttctcctgt ctgccgtgtc ggataggaga attttttcat ctttttttgt caagtaactt
     1381 tactttacaa aaaaaatgtg ttatcctaat atggttgatg aaaatcagta gattgaatcg
     1441 aatataaata cctaaaggag aaatcaaaat ggcagtacct gcacgtcgca cttcaaaagc
     1501 gaagaaaaac aaacgtcgta cacactacaa agtaacagct ccatctgtaa actttgacga
     1561 aactactgga gattactcac gttctcaccg tgtatcactt aaaggatact acaaaggacg
     1621 taaaatcgct aaagctgcat cagctgaata atagaaggga gataccatgc gcgtaaatat
     1681 tacacttgaa cacaaagaat ctggtgaacg cttgtacctt acttctaaaa acaaacgtaa
     1741 cactccagac cgccttcaat tgaagaaata ctcaccaaaa cttcgcaaac acgttgtgtt
     1801 tacagaagtg aagtaagcat tcaatacgaa tcaatacaga agaaaaacgc ttgatcagcg
     1861 ttttttcttt ttgttaattt cactatattg caagggaaat cattcgaatg actacatttt
     1921 ttgaaataag aatacggatt cggacgatta aaagtaagag aaactcaaat gtaagttggg
     1981 gttccttttt tgtatatttt tcaaataacg cctctacact atttgtagca aattcaccaa
     2041 ctacagttgc atcttagtta aaataagtta gaatatgtaa gtgagtacca gatataccaa
     2101 gacatcgtca ccatctaagg tatattcaaa atacaaaagt tgaccaacta gatttctg
//
LOCUS       DAVHIT010000138         2034 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVHIT010000138 DAVHIT010000000
VERSION     DAVHIT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.140"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..594
                     /locus_tag="VVN16_001999"
                     /pseudo
     CDS             1..594
                     /locus_tag="VVN16_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(828..1280)
                     /locus_tag="VVN16_002000"
     CDS             complement(828..1280)
                     /locus_tag="VVN16_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573298.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVRSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     TKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDAIDEVKSQITFK"
     gene            complement(1270..1569)
                     /locus_tag="VVN16_002001"
     CDS             complement(1270..1569)
                     /locus_tag="VVN16_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573299.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIFDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
BASE COUNT          661 a          388 c          353 g          632 t
ORIGIN      
        1 gtaatcaaac aacgatttgg cgaaatgtaa aaaaatatga ggagttcgga ctcgactccc
       61 tccttcaaga aacacgtggt ggtcgtaacc atgcatatat gacagttgag gaagagaaag
      121 tctttcttgc ccgccatttg aaggctgcag aggcaggaga atttgttaca attgatgcct
      181 tatttcaggc ttataaaaag gagttaggtc gttcctacac acgtgatgcc ttctatcaac
      241 tgttgaagcg ccatggttgg cgaaatatta cgccacgtcc agaacatcct atcaagaaga
      301 aaagaaagcg ctttaaaacc agtagatgtt ttcataaggt tcgcttgatg taccaagatg
      361 aagctggttt cggtagaatc agtaaactgg gatcttgttg ggctccaata ggagtaggtc
      421 cacatgtcca tagtcactat atacgagaat ttcgctattg ttatggagct gttgatgccc
      481 atattggaag atgtcatgaa tcaactccaa gatgtcatac aaggactgga gaaggaggtg
      541 ataaagtcca tcgttaatcg gagatggact agaatgcttt ttgaaaacag atgagtatta
      601 gaaataaatg aaaagtcaat atcaaaacaa tcagaatctt gatgatgcta aattcaaaaa
      661 aactacataa taattttaat aagggtaagt cgtaccaaga aaccccacca agataaaaac
      721 ttagtggggt ttggcgggga atataattat tctcccaatt attatcgcct ttatattatc
      781 tcaactctca ttaaacaaga gttccgcaat caaattgcga tactctatta tttaaaggta
      841 atttgggatt taacctcatc aattgcatcg tctaatgact tatccatgga aaaatcagat
      901 aatttctcag gatcattcac cattttttct aacagatggt ctaaatgatt gtctagaatc
      961 gaacggtctt tcgtgtagaa atgcagggaa tccccttgcc aagcgatggc taaaggaatc
     1021 tcttcttttt ctaaaaaagc tttaaatttc tctatatcaa ttggtttgtc taaaaaatct
     1081 tttttcagat taatcgtgtc aatcgaataa ggagattgta gcaattcttc taatttctgc
     1141 acccctatct tataggcgga atcctgtgct aaagcctgac gtctagacca ttctagaatc
     1201 tttaagagac ttcttacagt aaataaaaga ctacgctctg catattgaac tgccattcgt
     1261 tcctgttgtt cagaggacat ctgaggcctc cttcttcaca aatagcagct ttccttcttt
     1321 ataacgataa gctatcaatt tctgacgttg cttaatcgac ttgacaacct gcagtagatc
     1381 tctcgaataa ggttctcgaa gagtaacttc tactgctttt ccatcaaaga taccaacacg
     1441 tttaaattca atgtagtctt ttttgagatg tcctaagccc ataccaaaag gaaagtgatg
     1501 aatcaattgg atggtacaac cacctttatt tcccatttgt ttcaaatcat acaagtttac
     1561 taccttcatg attaactcct ttatctagtt tgtcgcatcg tttaagtctg gtaacgataa
     1621 actccgtgtc tgttagaaaa ttctcacaca cgtcttgtgc cagttgccct tcacagggaa
     1681 atactctcag tccctactta cacaggcacg ctaatcaaga cggagtgaat tcaattttca
     1741 aagaacaggt agctttatta tagataagag tagttgaaat ttttatcaca ttttttattt
     1801 tcctgtagag ctaaattcat actaaacaaa gattttgaaa taaaagacta acattgttac
     1861 ataacgttag catttcattt ccctaaaata agctaatttt taccagaaaa aatagcataa
     1921 aaatctagtt atccgcataa aaactggact tatcacactt tatcaaggtc aaaaccactc
     1981 aatttactac taatttacta cttatgaatg agctttgata cgacgattta tcct
//
LOCUS       DAVHIT010000139         2010 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVHIT010000139 DAVHIT010000000
VERSION     DAVHIT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.141"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(34..897)
                     /locus_tag="VVN16_002002"
     CDS             complement(34..897)
                     /locus_tag="VVN16_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5573300.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            1363..1701
                     /locus_tag="VVN16_002003"
     CDS             1363..1701
                     /locus_tag="VVN16_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU5573301.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKIKGTRITK"
BASE COUNT          631 a          293 c          427 g          659 t
ORIGIN      
        1 ctatgaaaag gtcgaggagc tccctcggct tttttatctt tccttcgctt ggtaatagat
       61 ctctacttct tttttgagat gagacagcat agaagtttcc tcggtcagct ccagaaggga
      121 gaaccctttt tggataagtt tagaatcgtc tgtacaaatc ccaatgcgga catagcagat
      181 agccagccta tcgtagcttt tcttattctt ggcgtcctcc agtaaagtgt agcagatttg
      241 cttacacatg tttttatctt gattgtatag atagagtgtg gaaaggttga gcaggattgc
      301 tattcgcaag tcatataaat gttgatagtt tttatagact tctaggcgtt gcaatatttt
      361 tccagtgatg agatggaggt attcaatggg aaaactgaaa agaatggtat tgaggatttt
      421 tagatcactc tcataccatg tatcttgttt ttcaattttt ctccaaagtt ttttgacaac
      481 ctgttctgcg tgatctgata gttcccctgc cccgtgttga cggatataaa tgacaacttc
      541 aagcatatct ctgatttctt ctatagggag atcgtggtgg gtcttgaggt agtcttggca
      601 tttttgaaat aaattgacaa gatcgctggt cccgataata gaacgcatat tgagataggt
      661 ttgcataatc tctgtgcgtt gacttggttg ataaagttga cagatatatt caaactcttc
      721 aaaactcata ttgatttgac ggagaagaaa ttccatattt tcatattttg gggttgtctt
      781 gccactttcg atttttgata agctggttct tgagagaaca cccccacaga cctcttcttg
      841 ggtcagcccc tttgactcac gtatttcttt atagaccttt ccgaaatcat aacgcatgat
      901 tttctccttt ctgtgaaaaa agttaacaaa taataaaatc ttgttaaaaa tattgtatca
      961 tagcaatgta aaaagtaaaa aagaaagtcg aaataaattt gtgaaaaaag ttaacgtaat
     1021 tttaaaatga gcttaataag aggtatgatg taggtagaaa ataaagatag gagaaaaagt
     1081 atgaataaaa aaatattttg tattctagta tgtattatat cattgattag tctggccata
     1141 atctttccat ggggctggtc gatataatgt aagggagctt gagttgttaa aggaggctta
     1201 gtccttggac aaaaatctgc tgtactacaa tgcttggaaa gatatcaaga acaaagaatg
     1261 atacgattga aactaaactg attcaagaac tactgggcag tgattgaggt ttgggtgaca
     1321 tcaaagaaaa gttagattaa ttctaaagaa ttgagtattt ctatgttaaa aaaatatcct
     1381 tgtacgatgc aacatgatca gtcagattgt gctgcggcag ttgtttcaac agttctttta
     1441 tcttacaaaa aggaactatc aatcatgaag attcgggaaa ttattggtac agacatgtat
     1501 ggaacgactg tcagtggtat tgtttcaggt ctgaataagt tgaattttac agtaaaagct
     1561 gttcgagtag cactggaaga tttgactcca aaattaacat ttcctgcgat tcttcaagtt
     1621 aagaatgatt taggtcaaaa tcattttgtg gtattacata gtataaagga gaaaataaag
     1681 ggaactcgta ttaccaaata attatgttgc tcttgagcaa gaggagatga tgtatcttga
     1741 tggggggggg cttatttaag caagagtgct tgtcaaggaa tttgcgcagc tttagctatg
     1801 agtccaggaa cttttatagc attgactgga gctgcagttt taacaaaaaa actaataaac
     1861 tatattaagg ttggaggcct tggaggttgg cttattggtg cagcagcagg tgtattggct
     1921 cttatctttt tgataaaaat ttgtgtagat actacgataa aggttctatt aaacagaatc
     1981 tttaaggcaa gtaaggtaat gaaaaggagg
//
LOCUS       DAVHIT010000140         2009 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVHIT010000140 DAVHIT010000000
VERSION     DAVHIT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.142"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..980
                     /locus_tag="VVN16_002004"
     CDS             <1..980
                     /locus_tag="VVN16_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5573302.1"
                     /translation="FSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVAT
                     IAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNF
                     EAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGME
                     KEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANY
                     LTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLG
                     KINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            970..1977
                     /locus_tag="VVN16_002005"
     CDS             970..1977
                     /locus_tag="VVN16_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5573303.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIALSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
BASE COUNT          710 a          288 c          360 g          651 t
ORIGIN      
        1 tttttagcgc ttgttcttct aattctgtta aaaatgaaga aaatacttct aaagagcatg
       61 cgcctgataa aatagtttta gatcatgctt tcggtcaaac tatattagat aaaaaacctg
      121 aaagagttgc aactattgct tggggaaatc atgatgtagc attagcttta ggaatagttc
      181 ctgttggatt ttcaaaagca aattacggtg taagtgctga taaaggagtt ttaccatgga
      241 cagaagaaaa aatcaaagaa ctaaatggta aagctaacct atttgacgat ttggatggac
      301 ttaactttga agcaatatca aattctaaac cagatgttat cttagcaggt tattctggta
      361 taactaaaga agattatgac actctatcaa aaattgctcc tgtagcagca tacaaatcta
      421 aaccttggca aactttatgg agagatatga ttaaaattga ttcaaaagcc ttaggtatgg
      481 aaaaagaagg tgatgagtta atcaaaaata ctgaagctcg tatatccaaa gaattagaaa
      541 aacatccaga aatcaaagga aaaatcaaag gaaaaaaagt attatttact atgattaatg
      601 ctgcagatac atcaaaattc tggatttata ctagcaaaga tccaagagca aattatttaa
      661 cagatttagg tctagttttc cctgaatcat taaaagaatt tgagagtgaa gatagttttg
      721 caaaggaaat ttctgcagaa gaagcaaata agataaatga tgctgatgta atcataactt
      781 atggtgatga taaaactctt gaagctttac aaaaagatcc tcttttaggt aaaataaatg
      841 caattaaaaa tggtgccgtt gctgtaattc cagataatac accgttagca gcctcatgca
      901 ctccaacacc actttcaata aactatacta ttgaagaata cctaaatctt ttaggaaatg
      961 catgcaaaaa tgcgaaataa aaaacaaata aacctaggca taatttttgt aatctgccta
     1021 ggtcttctta ttacaatatt tttgtcatta aagcttggaa caaaagaaat taatatcaga
     1081 gattttttag cagcttttgg aatgggtaat acaaatgatg attttattaa atcaattata
     1141 tataaaagaa tacctagaac tatttttgca attttagcag gttctagtct tgccataagc
     1201 ggtgtgttga tgcaatcagt tactagaaac ccaatagctg atccaggtat actcggtata
     1261 aacacaggag caagtcttag tgtagtaatt gctctttctt ttttaggaat ttcatcaagc
     1321 ataagccata taagttttgc aatcattggt ggcttagtaa gtgcaatttt tgtatacgcg
     1381 attgctgtaa gcggaaaagc aggccttacc cctataaaac tagccttatc aggaacttgt
     1441 gttagtatgg ctttaagcag ttttgtaagt tttttaattt taccgaataa taacgtctta
     1501 gacaaattta gattttggca aataggtagc cttggagcag ctacattatc ttctatatct
     1561 acactactac cttttataat tttaggtcac ttgatagcta tatttatttc atcagattta
     1621 aacgctttag ctatgggtga tgaaatggct gttggtcttg gagttaatgt taataggata
     1681 agatcacttg caataattgc aagtgtgctt ttatgttcaa gtattactgc aattggtgga
     1741 cctattggct tcgtaggtct tatagttcct cacttttgtg gcttatttat aagcaaagat
     1801 atacgcacaa tgaccatttc ttcatctttt ataggtgcag agctcttgct tatatgtgat
     1861 ataatcggcc gtatgttagg taaaccaggt gaaattgaag tagggataat tactgcaata
     1921 atcgggggtc cagtacttat ttatgtaact atgaaaaata gaggggttaa taactaatgc
     1981 aaaatttaat tataggtatt caaaaaaga
//
LOCUS       DAVHIT010000141         1990 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVHIT010000141 DAVHIT010000000
VERSION     DAVHIT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.143"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            51..782
                     /locus_tag="VVN16_002006"
     CDS             51..782
                     /locus_tag="VVN16_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573304.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHASYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            797..1948
                     /locus_tag="VVN16_002007"
     CDS             797..1948
                     /locus_tag="VVN16_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU5573305.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDNYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
BASE COUNT          727 a          254 c          379 g          630 t
ORIGIN      
        1 tttaagaaaa tgatataatt cattaaaaag tatttggagg aggaagaatt atgttgaatc
       61 aggatctctt tgattcgctt gaggcacaaa aaattgtaga tactttgatg aaagggcaaa
      121 aagattatgt agatgaacgt ctagaaaaaa gagagacaat gatagtgtcg aatggttatg
      181 catggacgcg acctaatcat attgatactg catttgcatc agcagatttg tttgagtata
      241 aattacaatt agcaggacag acttggggat atttagaatt tgaaacaaat acagaaaaat
      301 atgggaaagt attgttaatt ataaagggta agaagcgact tacgaaccaa tttcctttgg
      361 tacaaaaaaa taagagtggc tacttatttg aatatgctca gatgaataca ctttatctta
      421 atcaacatgc ttcctacaaa aatgatgaag atagtcattc ctttccaatt cagatggagt
      481 tagtttctga tgaaatgatt caagaaattg aacaagctac taaaaattcg aatatcgaaa
      541 aatttatgat tttaacttat gaggcggact cagaaaacaa tattatatct gtagatgttg
      601 ttatgcctga tgcacgaact ggtcagttac acttgattca agatttgtct gagtatattc
      661 aatcaagttc gtaccatttc gaagaagcta aatatcaaga tattcctaat ttttcagaat
      721 tatcggaaac agaagatttt gaaattattc caagaataga aaaacaagaa ggtcaaaagt
      781 agtaaggagt atgtaaatgt ttaatggtcg ggtattgaaa gaattacggc tgttaaatgg
      841 tttaagtaga gcagaattag ctcagagaat taatttaacg gaacaagcca tttggcagtt
      901 tgagtccaac gaaacgaaac ctaaattatc aaccaaaatg catttggcca accaatttca
      961 tgttgattta acttattttg aacaggaaga agagagcatt cgatttgatt cttctgtaat
     1021 tgcctttaga aatgcagacc tagcaacacg gaaaacaata gatattcaaa ctatgtattt
     1081 acataaggta gatagtttga ttgattattt tgaaagtttt gtaattatac ctaatattac
     1141 aattcatgac ctaagtaatg tagtgagtga atcttatcat aagggagaat ccattgagga
     1201 attggctctt tatgccaggg aaaaattagg tatttcaaaa gataatcatg atttgcttta
     1261 taaattagaa cgttcaggca tctatatcgt ggaacgatta attaatggcc aagctgatgc
     1321 ttatagcgca tggtcaaaat tgggaagacc ttatattgtg ttgggaacga ataaatcatc
     1381 tgtacgtcga aattttgact tagctcatga gctaggacat attcttttac ataaatataa
     1441 agatatgaat gaagatggcg atcgtttgga gcaagaagca aattattttg catcatgttt
     1501 tttattgcca aaagaagagt ttttagtcaa atttgaagag agggttggca agcgtgtcag
     1561 caatcctgat aattatattt tattgaagtc ggatttgaat gtttcgatac aggctttaga
     1621 gtatcgagct tttaagttag gattattgac tccaaagcaa cattcttact tttatcgtca
     1681 aattgcgcaa aaaggttaca aaatgattga acctttggat gatcaaattt ttgttaaaaa
     1741 accaagcaaa gtaaagagta ttctggacgt cgttttgagt aatcatctag tcagtctagc
     1801 gactataatg tctaaacaaa gtattcgttt acagtttata agcgaaatat tttcagtcga
     1861 aatgaaattt tttgatcagt atcaagaaga tagaagaaca gatcgatttg ataacatcat
     1921 ccctttgtac aaaagaaata atttataatt taactgtgaa ataatttcat gaaggatata
     1981 tggcagaaat
//
LOCUS       DAVHIT010000142         1959 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVHIT010000142 DAVHIT010000000
VERSION     DAVHIT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.144"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(248..1738)
                     /gene="lysS"
                     /locus_tag="VVN16_002008"
     CDS             complement(248..1738)
                     /gene="lysS"
                     /locus_tag="VVN16_002008"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU5573306.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINELFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(1866..>1959)
                     /locus_tag="VVN16_002009"
     CDS             complement(1866..>1959)
                     /locus_tag="VVN16_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU5573307.1"
                     /translation="DLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT          552 a          398 c          408 g          601 t
ORIGIN      
        1 tgaatctgaa caaatcaatt ggggaatcca agtcaatttc tagtactttt tgaacagttt
       61 cagcgtacta tcgtaaaatg aaataaaaca tgcacaaatt gattagggaa tttaaaccaa
      121 tttctaacaa tgtcttagaa atcaaagtgt actatttcaa cttcaatata ctacacttca
      181 ttttctcctt aaattcagtc tctttttgaa tcaaaaaatc ctctggtaag agccagagga
      241 taagaattta tttcattgtt gggaagagca atacatcacg gatagttgtt gtatcagtga
      301 ggagcatgca gagacggtcg ataccgattc ccaaaccacc tgttggtggc ataccgtatt
      361 caagagcttc aatgtagtca tagtcgattc ctgtcgcttc atcatcacca agttctttgg
      421 ctttagcttg ggcttcaaaa cggctaagtt ggtcgattgg gtcgttgagt tcagtaaagg
      481 cattaccgta ctccttagtc atgataaaga gctcgaaacg gtcagtaaag cgttggtctt
      541 caggattttt cttagcgagt ggagatacag ctactggatg tccatagaca aaggttggtt
      601 ggattaaagt ttcttcaaca aactcttcaa agaaggcatt gatgatgtga ccaacctcag
      661 tgtagtgttt ctcaactgga actttcttct cagcagcgat agctttagct tcttctaaag
      721 tcatgtcttg ccagaaatcg acaccagtaa tttctctgat agcatccacc atatgaacac
      781 gcttaaatag ttcgttaatc ttgatttcag taccttggta gttgactggg ccatcgccct
      841 taactgattt agcagcgtgt tggataatgc cttccgtcaa gtccatgatg tcttggaagt
      901 ctgcataagc ttggtaaact tcgatagaag taaactcagg gttatgagta gcgtccattc
      961 cttcgttacg gaagatacgg ccaatttcat agacacgttc cataccaccc acgataaggc
     1021 gttttaagtg aagctcagtc gcgatacgaa gcaccatgtc aatgttttgg gcattgtggt
     1081 gggtgataaa tggacgggca gcagcaccac cggcttcatt atgaagaaca ggtgtttcca
     1141 cttcaaggaa tcctttttgg tcaaggtaac gacggatttc agagatgatt tttgaacgag
     1201 tgacaaagcg ttcaaagctt tcacggttag aaatcaagtc aaggtaacgt ttacggtaaa
     1261 ttgtttcaac gtctgtcaaa ccatggaatt tctcaggaag aggacgaaga gccttagaca
     1321 agtgtgtgat gtgggttgcc ttgatagaga gttctcccat atccgtacgc atcacttcac
     1381 cttcgacacc aaggaagtca ccaaggtctg cttttttgaa gatttcgtag ttttcttcac
     1441 cgacagcatc cttacgaacg tagatctgaa tctggccttc gcggtcttga aggtgggcaa
     1501 aaccaacttt tcctttacca cgtttggtta ccaagcgtcc tgcgatagta gctgtttcgt
     1561 ttttatcgtg taattgttct ttatcgaggt tggcatattt atcttttaat tcttgtgaat
     1621 ttgcagtacg ttcaaaacgt tttccgaaag gatcgattcc ttgttcgcgg agcgcagcca
     1681 ttttttcacg gcgaacgatc tgctggtcat ttagttcttc catatgttct gtagacatgg
     1741 gatcctccta gttttactca aaattgaata cgatgagtca ttttttgcga tactcccatt
     1801 ttatcataaa taagcaagaa attcacggat aatctttaaa aactaaaaag aacctgacgc
     1861 taaaatcact ggaaagaagt gaaagtatct cccgccccac tagcgatata gccaaatgct
     1921 gctttaggaa taacttgttg cgccattggc tccaaatca
//
LOCUS       DAVHIT010000143         1908 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVHIT010000143 DAVHIT010000000
VERSION     DAVHIT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.145"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            8..1501
                     /locus_tag="VVN16_002010"
     CDS             8..1501
                     /locus_tag="VVN16_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU5573308.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1514..>1908
                     /locus_tag="VVN16_002011"
     CDS             1514..>1908
                     /locus_tag="VVN16_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5573309.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKD"
BASE COUNT          649 a          290 c          384 g          585 t
ORIGIN      
        1 ggaagatatg attacaggcg aattaaaaaa taaaatcgat cagctgtggg aaattctttg
       61 gacagaagga aacgcaaatc ctttaacaaa tattgaacag ttgacttatc tcttatttat
      121 gaaagatttg gatagtgtcg agcttggacg tgaaagtgat gctgaatttc tagggattcc
      181 ttatgaggga gtttttccaa aagataaacc tgaataccgt tggtcaactt ttaaaaatat
      241 aggagatgct caggaagttt atcgtttaat gactcaggag atttttccgt ttattaaaaa
      301 tctcaagggg gatacagatg atacagcctt ttcacgatat atgcgagaag ctatttttca
      361 aataaataaa cctgctacgc ttcaaaaggc aatttctatc ttagatgttt ttccaactag
      421 gggattagat gtagattttg ataatgacaa acaaagtatt actgatatcg gagatatcta
      481 tgaatatctg ttatcaaaat tgtcgaccgc aggtaaaaat ggacagttcc gtacacctcg
      541 tcacatcatc gatatgatgg ttgagttgat gcaaccgact atcaaagata tcatctcaga
      601 tcccgctatg ggttctgctg gcttcttagt atctgctagc cgttacttaa agcgtaagaa
      661 agatgaatgg gaaaccaata cagataatat caatcatttt cataatcaga tgtttcatgg
      721 aaatgatacg gatacgacta tgttgagact tggggcgatg aacatgatgc tacatggagt
      781 agaaaatcca caaatcagtt accttgactc gctgtctcaa gataatgaag aagccgataa
      841 atatactttg gttttagcaa atcctccttt taagggctca cttgactaca attcaacctc
      901 taatgacctt cttgcaaccg taaaaaccaa aaaaacagaa ttactctttc tttctctttt
      961 cttgcgaact ttaaaaccag gtggacgagc agcagttatc gtacctgatg gtgtcctttt
     1021 tggttcgtct aaagctcata aaggaattcg tcaggaaatt gtagagaatc ataagcttga
     1081 tgctgtaatc tcaatgccta gtggtgtgtt caagccttat gctggagttt caactgccat
     1141 tctcatcttt acaaaaactg gtaatggtgg tactgacaaa gtctggtttt acgatatgaa
     1201 agcggatggt ttaagtttgg atgataagcg acaaccgatt agcgacaatg atattccaga
     1261 tattatcgaa cgctttcatc atcttgaaaa agaagcagaa cgtcagagaa cggatcaatc
     1321 tttctttgtt ccagttgctg agataaagga aaatgattat gatttgtcta tcaataaata
     1381 taaagagatt gagtatgaaa aagttgagta tgaaccaaca gaagtcatat taaagaaaat
     1441 caatgattta gaaaaagaaa ttcaagctgg cttggctgaa ttggaaaaat tactcaagta
     1501 gggaggtggc tgtatgaaaa aagtgaagtt gggggaagtt tgtgagattt tatctggtta
     1561 cgcttttaaa tcatcacaat ttaatgataa caaaattggt ctccccttaa tacgaattcg
     1621 agatgtagaa agaggttttt ctgataccta ctttgaagga acttatcccg aagaatatct
     1681 tattaaaaat ggagacttat tgattacaat ggatggcagt tttatattga aaaaatggga
     1741 gggggattta gcgctactta atcaacgtgt ctgtaaaatt aaaattacgg ataaaagtgt
     1801 agatgaaggt tatatatctt ggttgattcc taaattttta aaagaaattg aagataagac
     1861 cccttttgtt acagtgaaac atctttcagt agctaaaata aaagatat
//
LOCUS       DAVHIT010000144         1857 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVHIT010000144 DAVHIT010000000
VERSION     DAVHIT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.146"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            266..1669
                     /locus_tag="VVN16_002012"
                     /pseudo
     CDS             266..1669
                     /locus_tag="VVN16_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT          666 a          236 c          373 g          582 t
ORIGIN      
        1 gattctctgg agttatggat ctcgttccaa tatgtgcacg ttggaatgtc agtgcttata
       61 tggggggaat ccaaaggatt ctcccttttt attttaataa gtctgtctag ctatagatat
      121 attaagtttg atagaatttc ttggagattt tgttatggat tataatttca atttggagca
      181 tcctttcttt ttcactaata atgattatag tacagatact agcataaagt actaagtaag
      241 tctgcctttt aattggcacg aagtaatgaa taatgatgag tgggtttatc aataccctat
      301 tggaaaattt gttgaaagac aagggtggaa aatacacatt agttcagagt ataatagttc
      361 acacgaattg ttacaagatg tagcaaaaat ttgccatgaa atgcgaattc catttaaaca
      421 tcttagtaca gaagacaaat ttatcatgcg aaacgggaaa ttggttagtc gaggattttc
      481 tggtaaattt attacatgtt atccaaatca aaatgaatta gagtcggttt tgcaaaggtt
      541 agaaagtgcc ttaaagcaat ataatgggcc atatatttta agcgataaac gttgggatga
      601 agcaccaata tatctgaggt atggtgtgtt taggccgtca agggatgatg aaaagaaggt
      661 tgttattgat gaattaattg ttggcgatga agttgttaaa gatgaacgtt taccagtatt
      721 taaaattcca aaaggtatag tgccccctga ctttttgaat aaatggcttg acaagaaaga
      781 taaaaaacag ggagactttc cctttattat agataatgca attagatttt caaacagtgg
      841 tgggatatat aatgctagac taaaagaaga tgggaaaaaa ataattttaa aagaggctag
      901 accgtataca ggtctaggat ttgatggtac ttattcttca gagagattag cttcggagtg
      961 caaagcacta aaaattctaa atgaatggag tgagatgcct aaaatatact ggtaaggtaa
     1021 gatctgggag cacaccttct taggaattga acatatgaag ggcgttccac taaatagatg
     1081 ggttaccaat aattttcctt tatatgaagt tgttgataag accaaagatt atctattgag
     1141 agtttcaaag atagtagaaa aactcatcga tttaacgaat aagtttcatt ctgaaaatgt
     1201 atatcatcaa gatttacatt taggaaatat cttggttaag gatgaagatg agattagtat
     1261 aatagactgg gaacaggctg tcttttcgaa tgatgaaaaa gttgtacata aagttgcagc
     1321 ccctggtttt agagcatggc gagaaacact tcccagtgag atagattggt atggtattag
     1381 acaaatagct cattatttat acatgccact agtaactaca tcggatttga catacaacta
     1441 tgtaagtcaa accaggatag aaggaaaaaa actatttgag tctttgggtt atacaagaga
     1501 acatatagat tatgtagaaa gtttattgtc atatctagat tctaaatgtc cacagattga
     1561 gaatatttcc aggaaaaaag ttttaaaacc catgcatgag attagaacta tcgagagtga
     1621 gcaagatata caagatttta ttataaaagc ttttctttta gtgaactgat aggtattttc
     1681 ttagcaaacg ataaagtttt agggccgata caatcaattg cctattcatt aaataagata
     1741 aatacaacca aagatttaag gaaaccgttt ttaaaatact taagtggaga gaagaatttt
     1801 atagacgctg aacatgataa taacggactg tatacttcat caatagatgt gatacac
//
LOCUS       DAVHIT010000145         1799 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVHIT010000145 DAVHIT010000000
VERSION     DAVHIT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.147"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            33..905
                     /locus_tag="VVN16_002013"
     CDS             33..905
                     /locus_tag="VVN16_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5573310.1"
                     /translation="MIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQT
                     MLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKD
                     GFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLI
                     VSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPI
                     ACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSP
                     YLIYLLIKMNRRNI"
     gene            905..1699
                     /locus_tag="VVN16_002014"
     CDS             905..1699
                     /locus_tag="VVN16_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573311.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          620 a          282 c          306 g          591 t
ORIGIN      
        1 ttatcggaga tgaaagttat tctttttcaa ctttgattaa agtcttaaat agtgaaactg
       61 ttcctggagc tagtttttcg attatggaaa ttagattacc aaaattatta gcaggaatta
      121 tagctggctg gtcttttgga ttggcaggat ttatctttca aactatgtta agaaatcctc
      181 ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca
      241 tattggtatt aaaaacaaat agtttaacta ctggaattat ttcaataact tgtggactaa
      301 catcatcttt aatattattt ttactagcta aaaaagatgg tttttcagca gcaagactga
      361 taatattagg tattggtttt caagctgtca caagagcagg cacctcattt ttattgttga
      421 aagtagcaag atatgaatta caagaagtta tgagatggct cagtggctct ttatctttta
      481 caaagttaga tgacatacct cttgttctaa tagtaagtat tattgctact atattagttt
      541 tattttttaa taaaagacta gaaattattg aacttggtga agaaatagca atcggacttg
      601 gagcaaatcc cgagctttca aggcttgttt taattttttg cgctgtatct ttaactgctt
      661 tttctacttc aattacagga ccaatagctt gtatatcttt tttagctggt cccatagcct
      721 taaatattgg caagaaaaga agcccaatat tagctggatt ggttggaatt ttactagttt
      781 tgttatcaga catattctct caaaatattt taccagctag atatccagta ggtgttgtaa
      841 ctggcttgtt aggttcacca tacttaatat acttactaat aaaaatgaac aggaggaata
      901 tataatgaaa ggtctgtggt caaataatct tacctgcggt tatgatgaaa aaataatctt
      961 ggaaaatata aatataaaaa tacctgaaga aaaaatatca gttattattg ggtcaaatgg
     1021 ttgtgggaaa tcaacactca ttaaaacctt gtctcgactt ataaagccat tagagggaga
     1081 agtattgctc gataataaat caattaattc ttataaagaa aaagatttag caaaacacat
     1141 agctatatta cctcaatctc caataatccc tgaatcaata acagtagctg atcttgtaag
     1201 ccgtggtcgt ttcccctaca gaaagccttt taagagtctt ggaaaagatg accttgaaat
     1261 aataaacaga tcaatggtta aggccaatgt tgaagatcta gcaaataacc tagttgaaga
     1321 actttctggg ggtcaaaggc aaagagtatg gatagctcta gccctagccc aagatacaag
     1381 tatcctactt ttagatgagc caactactta cttggatatc tcatatcaaa tagaactatt
     1441 agacctcttg actgatctaa accaaaaata taagacaacc atttgcatga ttttgcacga
     1501 tataaatcta acagcaagat acgctgatta cctatttgca attaaagaag gtaaacttgt
     1561 tgcagaggga aagcctgaag atatactaaa tgataaacta gttaaagata tctttaatct
     1621 tgaagcaaaa attatacgtg accctatttc caattcgcct ctaatgattc ctattggcaa
     1681 gcaccatgtt agctcttaat aattgaatat tggaattcat ttaaaagtcg caaattttga
     1741 actgaccctc aaaagttagg ccaaaaaact aactaaagga ggtcagtttt ttaatggca
//
LOCUS       DAVHIT010000146         1786 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVHIT010000146 DAVHIT010000000
VERSION     DAVHIT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.148"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            185..694
                     /locus_tag="VVN16_002015"
     CDS             185..694
                     /locus_tag="VVN16_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU5573312.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKEKVKP
                     NKKDMTREI"
     gene            821..1543
                     /locus_tag="VVN16_002016"
     CDS             821..1543
                     /locus_tag="VVN16_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU5573313.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIDIEKDIEIDKEREIDKKSTP
                     IFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEE
                     DKDKLRQKGLNKKMNYDVGESL"
BASE COUNT          872 a          240 c          298 g          376 t
ORIGIN      
        1 ggtggaaagg tagtgtaagc tatttttaaa agtataaaag gtacaaagac ctaagaaata
       61 tataaaaaca tcaaaataaa gtattccacc accaagaaca ataaaataaa aacaagcaaa
      121 gggaagtata gaaaaattaa aacctatact tccctttttt aattcaaaca aaggagagaa
      181 aagcatgata aacgaagaaa taagcaagga agcaggtcaa gcagcacaaa ccataatatc
      241 atacacaata aaggcaacaa aagaatcaat caatttagaa aaagaaataa gaaaaaagat
      301 gaatgaaact ttagaaaaag caaatggaaa cttaaaaagt cttatgggcg atgaaatgaa
      361 aataaaagac ctctacaaga aaggacaact agaaaatata agcatagatc aaatcgacct
      421 caaagactta aaaaaagaac taaacaaact tggagtaagt ttctcagtaa tgaaaaacaa
      481 agaaagcaaa aactatgaaa tattcttcca agccaaagac ataaaagtaa tggaatatgc
      541 ctttaagcaa gtcatagcca aggaaaataa aaaagaaaaa gaaagtatcc taaaacaaat
      601 aaagaaatac aaagacctat ccaaaaacaa agataagaca aaagaaaaag aaaaagtaaa
      661 accaaacaaa aaagatatga ccagagaaat ctaaagggag taacgaaaag ttacaccctt
      721 aaataaccat aataacaaga cttccgaaaa acagaagtcc agaattccga aaatcggaaa
      781 tcaagaattt cgattatcga aaatcaagaa aggagcagat atggcaacaa acaaaagata
      841 ctattggata aaattaaaag aagaattttt cacagacaaa aggataaaaa ggttaaggag
      901 aatatctgga ggagatacct atacgataat ctacctaaaa cttctactac taagcctaaa
      961 agatgagggc aaactctatt atgacggagt agaaagtgat tttataaagg agttagcact
     1021 aaccatagat gaaacagacg atgatgtaat ggtaacagta aattatttaa tcaatcaagg
     1081 cttattagaa gttgtaacag aaaatgatga atactaccta actgaaatac caaacttaat
     1141 cggatcagaa acagcctggg cagagaaaaa aagaagatac agacaaaata aacagaggac
     1201 attgtcctta atgagtccaa cccatgtccg acaagagata gatatagaga aagatataga
     1261 gatagataaa gagagagaga tagataaaaa gtccaccccc attttttatg gagaattcaa
     1321 aaatgtaagg ttaagcaaag aagaatataa aaatctaaaa gaaaaattaa actcacacac
     1381 agatataatg ataaataaac tatccagata catggaaagc agtggtaaga cctatcaaaa
     1441 ccactatgtg acaatcttaa aatggtatga agaagataaa gacaaactaa gacagaaagg
     1501 tttaaataaa aaaatgaatt atgatgtagg agaatcttta tagataaaaa gaggtggaaa
     1561 ggcactatta aagactttaa gtctaaaaag gtatcaaagt atgccagaga taaaaaaata
     1621 taaatatgac actttaaagg cgattagaaa attaaagaaa tgctagaaga cttttagatg
     1681 acagttacca agaggatcta attttcactg ctacttatgc agtgggaatt ggaccttttt
     1741 atataagcaa attctatttc aaaattggtt accaagaaag acttct
//
LOCUS       DAVHIT010000147         1671 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVHIT010000147 DAVHIT010000000
VERSION     DAVHIT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.149"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..>1671
                     /gene="phtD"
                     /locus_tag="VVN16_002017"
     CDS             <1..>1671
                     /gene="phtD"
                     /locus_tag="VVN16_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU5573314.1"
                     /translation="YLKDAAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAARAQGR
                     YTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELAAAEAYWNGKQGSRP
                     SSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSLLRELYAKPLSERHVESDGLI
                     FDPAQITSRTARGVAVPHGNHYHFIPYSQMSELEKRIARIIPLRYRSNHWVPDSRPEQ
                     PSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKD
                     LSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGR
                     QVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQVA
                     KLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEK
                     GLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHY
                     DHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          530 a          363 c          351 g          427 t
ORIGIN      
        1 tttaccttaa ggatgcagct catgcggata atattcggac aaaagaagag attaaacgtc
       61 agaagcagga acgcagtcat aatcataact caagagcaga taatgctgtt gctgcagcca
      121 gagcccaagg acgttataca acggatgatg ggtatatctt caatgcatct gatatcattg
      181 aggacacggg tgatgcttat atcgttcctc acggcgacca ttaccattac attcctaaga
      241 atgagttatc agctagcgag ttagctgctg cagaagccta ttggaatggg aagcagggat
      301 ctcgtccttc ttcaagttct agttataatg caaatccagc tcaaccaaga ttgtcagaga
      361 accacaatct gactgtcact ccaacttatc atcaaaatca aggggaaaac atttcaagcc
      421 ttttacgtga attgtatgct aaacccttat cagaacgcca tgtggaatct gatggcctta
      481 ttttcgaccc agcgcaaatc acaagtcgaa ccgccagagg tgtagctgtc cctcatggta
      541 accattacca ctttatccct tactctcaaa tgtctgaatt ggaaaaacga attgctcgta
      601 ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaagacca gaacaaccaa
      661 gtccacaatc gactccggaa cctagtccaa gtctgcaacc tgcaccaaat cctcaaccag
      721 ctccaagcaa tccaattgat gagaaattgg tcaaagaagc tgttcgaaaa gtaggcgatg
      781 gttatgtctt tgaggagaat ggagtttctc gttatatccc agccaaggat ctttcagcag
      841 aaacagcagc aggcattgat agcaaactgg ccaagcagga aagtttatct cataagctag
      901 gagctaagaa aactgacctc ccatctagtg atcgagaatt ttacaataag gcttatgact
      961 tactagcaag aattcaccaa gatttacttg ataataaagg tcgacaagtt gattttgagg
     1021 ctttggataa cctgttggaa cgactcaagg atgtctcaag tgataaagtc aagttagtgg
     1081 atgatattct tgccttctta gctccgattc gtcatccaga acgtttagga aaaccaaatg
     1141 cgcaaattac ctacactgat gatgagattc aagtagccaa gttggcaggc aagtacacaa
     1201 cagaagacgg ttatatcttt gatcctcgtg atataaccag tgatgagggg gatgcctatg
     1261 taactccaca tatgacccat agccactgga ttaaaaaaga tagtttgtct gaagctgaga
     1321 gagcggcagc ccaggcttat gctaaagaga aaggtttgac ccctccttcg acagaccatc
     1381 aggattcagg aaatactgag gcaaaaggag cagaagctat ctacaaccgc gtgaaagcag
     1441 ctaagaaggt gccacttgat cgcatgcctt acaatcttca atatactgta gaagtcaaaa
     1501 acggtagttt aatcatacct cattatgacc attaccataa catcaaattt gagtggtttg
     1561 acgaaggcct ttatgaggca cctaaggggt atactcttga ggatcttttg gcgactgtca
     1621 agtactatgt cgaacatcca aacgaacgtc cgcattcaga taatggtttt g
//
LOCUS       DAVHIT010000148         1624 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVHIT010000148 DAVHIT010000000
VERSION     DAVHIT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.150"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..321
                     /locus_tag="VVN16_002018"
     CDS             <1..321
                     /locus_tag="VVN16_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573315.1"
                     /translation="YFYSQVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQN
                     SDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKKL
                     EISR"
     gene            377..1072
                     /locus_tag="VVN16_002019"
     CDS             377..1072
                     /locus_tag="VVN16_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573316.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1084..1500
                     /locus_tag="VVN16_002020"
     CDS             1084..1500
                     /locus_tag="VVN16_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573317.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT          506 a          252 c          381 g          485 t
ORIGIN      
        1 tatttttatt cgcaggtaaa agttgtagat aaacttactg attctgaatc gaaaaaatac
       61 ggtgaatata ttgatttgta cgaatcgtgt cgtcagaaca atttaagtgt tctacttaac
      121 tttcttcaaa attcggatga taaacgagga gttattgcta gtaactcagt gagaattatg
      181 tttgaatctt tggataaagc agaccaaatt gcggccctga atatttgtag aaactatgta
      241 gcacgagaaa atgttttaaa taaagaagat gcagaaaaat tgttaaatga aattgataaa
      301 aaacttgaaa ttagcagata ggacaattca aatagagttg agtaaaaagt ttgaaaagtg
      361 aaacaaggag aaagctatgc cagtaaatga atatggtcag atgattggtg agtcaatgga
      421 aggttataca cccggtgcac tgccttctat tgatttctta gaagggcgtt atgctaggat
      481 agaggctctc tcggtagaaa aacatgcgga ggatttgcta gctgtttatg gcccggatac
      541 tcctcgggag atgtggacct atctttttca ggagccagta gcagatatgg gggagctggt
      601 tagcctttta aatcagatgt tggctcgtaa ggaccgtttt tactatgcaa tcatagacaa
      661 ggcgactggt aaggctttgg gaactttttc tctcatgcgc attgaccaga ataaccgagt
      721 aatagaagtc ggagcagtca ctttttctcc agagctaagg ggtacacgga taggaactga
      781 ggctcagtat ctcctagctc gctatatttt tgaggagcta aactatcgtc gctacgagtg
      841 gaaatgcgat gccttaaatc tgccatccag acgagttgcg gagcgtttgg gatttgtcta
      901 tgaaggaacc ttccgccagg cggtggttta taaggggcgt acgagagata cggattggtt
      961 gtctatgatt gatatggact ggcctcaagt caaagatcgt ttggaaacat ggctgcgtcc
     1021 tgaaaatttt gataaaaatg gacagcagta caagagcttg agagaacttt aagaggtgtt
     1081 gagatgatta ctattaaaaa gcaagaaatt gtcaagctag aggatgtttt gcatctctat
     1141 caggctgtcg gttggacaaa ttatacccat caaccagaga tgctggagca tgccttatcg
     1201 cattcattag taatttatct ggcacttgat ggtgatgctg tggtgggctt gattcgtttg
     1261 gttggagatg gattctcatc agtttttgta caggatttga ttgttttacc aatctatcag
     1321 cgtcaaggga ttggtagtgc cctaatgaaa gaggccttaa aagattacaa agatgcttat
     1381 caagtccagc tggtgacaga agagacagaa aaaaacgtgg gattttatcg ttctatgggc
     1441 tttgaaatct tatccaccta taattgtata gggatgactt gggcgaatcg agaaaaataa
     1501 caaaacttgt tttttcttaa gcaaaattta aggatggtct agtattatat agtcattaaa
     1561 taaagacctc ctaactttat ttaataaaat cctaaacttt tttcatcaca atctcctaat
     1621 gaag
//
LOCUS       DAVHIT010000149         1610 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVHIT010000149 DAVHIT010000000
VERSION     DAVHIT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.151"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(105..1241)
                     /gene="lctO"
                     /locus_tag="VVN16_002021"
     CDS             complement(105..1241)
                     /gene="lctO"
                     /locus_tag="VVN16_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU5573318.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT          468 a          370 c          294 g          478 t
ORIGIN      
        1 gatttgtcct attcttattt cattttacta tagttttcga tagcaattta ttcttccaat
       61 acacgaagaa aaacctccac attcagtgga ggcaatctgt tttatcaata caattttaag
      121 tcacgagggt caactgggaa ggttgggttg tatggattgt gacggagctt aaagtgtttg
      181 acatcttcaa tggtctgagc tccagacaat tgcataactg tcttcaattc cgcattcaag
      241 tgttcaaaga cttgacgcac accgacacta ccaccgagag ccaagccata gataacaggg
      301 cgaccaatag ctaccaagtc tgctcctgaa gccaaggctt taaagacgtg ttgaccacga
      361 cgaacaccag agtcaaagac aatcggcaca cgtctatcaa ctgcttctgc cacttcttga
      421 agcgagtcaa aggcagctgg tccaccgtcg atttgacgac caccgtggtt ggttacccag
      481 ataccagaag ctcctgcagc aagcgaacgt tcaacgtcct cacggcattg tggtcccttg
      541 acatacacag gaagaccaga gtattcagcg ataaattcta catcgcgtgg agacaagcgt
      601 tgtttagctg atttataaac aaagtccatt gatttaccag caccttctgg caggtattct
      661 tcaacaatcg gcatgccaac tgggaagaca aaaccattac gcttatccac ttcacgattc
      721 ccccctacag tagcatctgc cgtcaagaca atcgctttat aaccttcagc cttcacacgg
      781 tccatgatgt ggcggttgat accgtcatcc ttactaaagt aaaattgaaa ccaatgaggt
      841 gtcccttgaa gggcttcaga aatctctgga aggtcaacag tagagtaaga actggttgta
      901 taaagagaac caaactcatg cacaccacgc gcagtcgcca cttccccctg ttcattcgct
      961 aatttatggg ccgcaacagg cgccataatg attggtgaag atagtttttc acctgcaaat
     1021 tcaatctctg tacttggatt ttcaacatcg caaagtgtat gaggaacgat gagtttgtgg
     1081 ttaaaggcgc ggatattctc tcttaaagtg aaagtatctt ccgccccact agcgatatag
     1141 ccaaatgctg ctttaggaat aacttgttgc gccattggct ccaaatcata ggtattgatg
     1201 aaatctacat ggccttctgc attgcttgtt ttgtatgaca taaactgtcc tccttgatta
     1261 agtaagcgtt tgctttttac tatacaacta taataactct tttttcaatc attttcaaga
     1321 gttttgctta aaaaccatct tattttttca tacaatatac tagccctact ttccttaaac
     1381 tctaaacttt agtctatatt aagtgaatcg ttgagatttt ttgctgccag gcatttgaac
     1441 tactttttta tttgctgatt ctatctacta atactataaa aaaacaagtg aataagacac
     1501 cagaacatcc tctaatagga cctatggact tatatcagtc ccatatatac tgtttaagca
     1561 agatactatc ctagttgacg ctaacttcct atcccactta ttaatttttt
//
LOCUS       DAVHIT010000150         1589 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVHIT010000150 DAVHIT010000000
VERSION     DAVHIT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.152"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            37..720
                     /locus_tag="VVN16_002022"
     CDS             37..720
                     /locus_tag="VVN16_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000507009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573319.1"
                     /translation="MGFSPGVAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYD
                     KPLLLPLIPFSIIIALVQKLYRIENQQLFLLKSKVLTILESKKDCEFNLQDYHEIWKL
                     QSKSELPCVALSYISLIKPYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLY
                     LRHYNEKNKYESMLKKILEVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYM
                     GIKHVSIRR"
     gene            1097..1471
                     /locus_tag="VVN16_002023"
     CDS             1097..1471
                     /locus_tag="VVN16_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU5573320.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          543 a          207 c          288 g          551 t
ORIGIN      
        1 gctaggaaat actatcgaat tctctttcac caactaatgg gatttagtcc aggagtagca
       61 gtgttttata aggagagtca aacaactaag aatctgttta aattttacta cttcttgtac
      121 tttacaacac taatttcata ctattttttc tttacttttg tgtatgacaa accgttgctt
      181 ttgcctctta ttccattttc tattatcata gcattagtac aaaaacttta tagaattgag
      241 aatcaacaat tatttctgtt aaaatcaaag gtactaacaa tactggaatc taagaaggat
      301 tgtgagttta atcttcagga ttatcatgaa atttggaaat tgcaatcaaa atctgagttg
      361 ccatgcgtgg ctttatctta tatcagtttg atcaagccat atctttcaga gagcgtgcgt
      421 gaacaaattg atttattaga agttaagcga tttaaaaaaa taaatcatcc aatatcactt
      481 tatggtatgt tggatgttat aaaattaaat ctatatttac ggcattataa tgaaaaaaat
      541 aaatatgaat caatgttaaa gaaaatttta gaggtaagac cagactttgt tttaatcgag
      601 cagaatattg atgatagttt aaatagctcg caaccattat ctttatcttt agccatttca
      661 gaaatccaac ttttgcttga agtgtatatg gggatcaaac atgtttcaat taggagataa
      721 aactagaata atatttgcta ttataggggc aagttgcata ttagtcagta tgtatcttat
      781 acctctcatt aacacggata ataaattttg ggaaatactt ttatttataa taggtgtatt
      841 gctaattgga attgcgttac taagtcaaaa aaaataaatt ttgaaatagt attagtgagt
      901 tcgcaatcat ttattgctag tggtttagaa ctgttaagtt tcaagcaata attggtttta
      961 aaccttaggt aacctataaa aacaaataag gtagagaaag gatatcttat ctaggtagct
     1021 aagtgataaa aacacagtcg tctatcactt gttaggaaac catcctaact atgcttaata
     1081 aaaaaggaga aaataaatgg aactcgtatt accaaataat tatgttgttc ttgagcaaga
     1141 agagatgatg tatcttgatg ggggattttc tattccgaga tggcctgttg caacagccat
     1201 taatatagct tttaatggtg ttttaggtgg aggagcaatc agtctagtta gaaattatat
     1261 tcgtaattat ggtttgcggc gagttacaag cgcaattgct ggagcagctg caagatatgt
     1321 tggggtacga gttgcaaata gagtggcagg atttgcactg tctgctatta atggatttgc
     1381 agcttggatg tcaattggcg atgctattac aacaatctgg gccaacaatg atgtaaatag
     1441 gagagaccca aatttaaacg ccttgtggta actagatatg ggtattaaaa tggaaaaaat
     1501 ttttgttatt attttttttg tatgtttatt tatatcatcc ataacttttt tagcctatga
     1561 ttttgttagc gaagaaataa aaaagttga
//
LOCUS       DAVHIT010000151         1577 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVHIT010000151 DAVHIT010000000
VERSION     DAVHIT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.153"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            27..1373
                     /gene="gdhA"
                     /locus_tag="VVN16_002024"
     CDS             27..1373
                     /gene="gdhA"
                     /locus_tag="VVN16_002024"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU5573321.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHGGSIWTYAGNYDIALPCATQNEINGEAAKR
                     LVDQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          474 a          303 c          329 g          471 t
ORIGIN      
        1 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
       61 ttgaaactgt gaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      121 ttttcaacac tttagaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      181 tggcacgtat tactgagcct gagcgcgtgg tttctttccg tgttccttgg gttgaccgtg
      241 atggaaaaat tcaagtaaac cgtggttacc gtgttcaatt caactcagct gttggaccat
      301 acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat
      361 ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt aaaggtggat
      421 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      481 tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg
      541 ttggtggacg tgaaattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg
      601 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      661 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
      721 ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatatgct cttcaaaaag
      781 caactgaact cggtgcaact gttatttctg tttctgactc aaatggttat gtcatcgatg
      841 aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga
      901 ctgagtatgc agctgaaaaa gcaactgcta cttatcatgg aggttctata tggacttacg
      961 ctggtaacta tgatatcgct cttccatgtg cgactcaaaa cgaaatcaac ggtgaagcag
     1021 ctaaacgttt ggttgatcaa ggcgttatct gtgtatctga aggtgccaac atgccaagcg
     1081 accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct gccaaagctg
     1141 ccaacgctgg tggtgtagcc gtttcagccc ttgaaatgag ccaaaatagt cttcgcctct
     1201 catggactcg tgaagaagtt gatggccgtc tcaaagacat catgacaaac atcttcaaca
     1261 cagctaaaac gacttcagaa acatatggtc ttgataaaga ctaccttgca ggagcaaaca
     1321 ttgctgcctt tgaaaatgta gcaaacgcta tgattgccca aggtattgtt taagatttat
     1381 ttgcttaatc ctcaatcact acgattgggg atttttagtt gagttagaca gttgggaaat
     1441 tctaattaaa tgaaataaaa actgaacaaa ttgattgcgg aattcaaacc aatttctaac
     1501 atatttctag aaattaattt gacttttcta atcgatttgt tcatatctta tttcaattta
     1561 ctatacttac ttaaccc
//
LOCUS       DAVHIT010000152         1543 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVHIT010000152 DAVHIT010000000
VERSION     DAVHIT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.154"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            25..342
                     /locus_tag="VVN16_002025"
     CDS             25..342
                     /locus_tag="VVN16_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEU5573322.1"
                     /translation="MMASKRLSIEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNK
                     RLIEKGAVFESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKD
                     EIT"
     gene            656..>1543
                     /locus_tag="VVN16_002026"
     CDS             656..>1543
                     /locus_tag="VVN16_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU5573323.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDI"
BASE COUNT          724 a          186 c          274 g          359 t
ORIGIN      
        1 gttttttatt ttaagaaagg atacatgatg gcaagtaaaa gattaagtat tgaagaacag
       61 attgaaaaga aagaagaaag cattaagcaa ttacaaaatc aaaaaagaca gttgaagaaa
      121 aaacttaatg aacaagaaag aaaagcaaga aacaaaagac ttatagaaaa aggtgcagtg
      181 tttgaaagta tttttgaaga aagtattgat ttaacaaaag atgaatttta taagttaata
      241 aaaacgttaa atgatgaaga aataagatta aacataatgg aaattttaga agaaagaata
      301 gatgataatg tagaaaaatc atctaaagat gaaataacat aattgtccct tggttacaag
      361 ggcgcaatta tacaccctaa agggtgcttg cgtcctgcgg agtcaaaccc ttgcagggag
      421 caacaaccat tcgcttttta aaagtcaagg gtaaataaac tcgctaaggc tcgcccttga
      481 cttttaaaat ttgaaatgaa agtaacaatt aagccgacat actgaaacaa caaaaattaa
      541 ttcagtagtc ggctttttta attctatcat ttcaaaacgc tcattcacaa agaggatata
      601 aaaaaactat taagcctcca cctaaaacaa caaagaaaga aaggaagtga taaaaatggc
      661 agacagtttt catttttcag taaacataat atcaagagga aaaggcaaaa gtgcagtagc
      721 aagtgcagca tatataagtg gagaaaaaat aaaaaatgaa tgggacggag taacccatga
      781 ctacacaaga aaagaaaaaa tattagtaaa aaatataata ttgcctgatc atataccaaa
      841 agaatttaat gataggtcta ccttatggaa taaagtagaa atggcagaaa aaaattctaa
      901 tgcacaacta gcaagacaat tcataatagg actaccaaaa gagttatctt taagtgaaaa
      961 taaaaacctg gttgaaagat atataaaaga aaatttaaca tcacaaggaa tgatagtaga
     1021 ttatgcaatt catgatgaga gtcaagataa aaatggaaat atccattgtc atataatgac
     1081 cataatgcga ccaataaacg aaaaaggaga gttcttagcc aagtcaaaaa aagaatatat
     1141 cctagatgaa aaaggagaaa aagtattaaa caaaaatgga aaaccaaaga caagaaaagt
     1201 agaactaaca acctggaacg atacaggcaa tgtagaaaaa tggagagaaa atttttcaga
     1261 tctttgtaac aaatatttag aaagagcagg agcagaaaaa agagtagacc ataggagttt
     1321 taaaagacaa aattcagatt atctaccgac aatccattta ggatcagcag caagtgcaat
     1381 ggaaagaaag ggaatagaaa ctgacaaggg aaactataat agagaaataa gaaaatataa
     1441 tcaacttgta aaaacaataa aagaagagat aaaaacatta aagggttgga taggaaattt
     1501 attagataac ttatctactg cttatgaaaa atttaaggat atc
//
LOCUS       DAVHIT010000153         1492 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVHIT010000153 DAVHIT010000000
VERSION     DAVHIT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.155"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..439)
                     /locus_tag="VVN16_002027"
     CDS             complement(<1..439)
                     /locus_tag="VVN16_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU5573324.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQ
                     KFSPEMMVMAKGINVGISTIYYWIHHGKLGLSKQDLLYPRKGKA"
     gene            complement(897..1295)
                     /locus_tag="VVN16_002028"
     CDS             complement(897..1295)
                     /locus_tag="VVN16_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573325.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          431 a          294 c          238 g          529 t
ORIGIN      
        1 gcgcttttcc ttttctaggg taaagcagat cctgtttgct taaccccaat tttccatgat
       61 gaatccaata gtaaatggtt gaaattccca cgttaatccc tttagccata accatcattt
      121 caggcgaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc ttggtcacgc
      181 ttgatttcct gaccgagcgc ttgcgattgt tttcataaga ctgttgagca tagtcggcag
      241 aataaacctc tttgaagcgc ccttttccaa gacattgtcg gactgtccca cgcttgattt
      301 cagtgtggat agtttgagga gcttttccaa gtagagaggc aatttctcta tttgattttc
      361 cttctttttt ccatcgttcg attaagcgac ggctatcgat tgtcaaatgt ttaccttttg
      421 tagtataatt gtcttgcatt tctgtgcctt tcttgtgttt gtggttgaac aacaagtata
      481 acacagaggt gttttcttat gcctacaaga gttttcatta tttccatttt ctttcggatt
      541 tcactctatt ctgaaaaact tgtgtatatt tactgaagct agcaagtctt acctgtaaat
      601 ttaatgaaag caacacaaaa tcctagaggg gaatctcgga ttaatgtcaa tttaaaaaga
      661 tagagagttt ttagtttaaa taaattgttt taaaatatca acaacatcac ttcttttctt
      721 aacctgataa gccttgattc ctaattttgg agctacgatt atattgtcct caatatcgtc
      781 tagaaagaca caatttctag gttataactg gtatttatcg atagttactc atatacatca
      841 gtacacctcc atacttatgt gcgagcctct ctttgtatta tacctccata ctcaccttac
      901 agattctttt ggtaataata tctttgccta atgtagagat agtcttgcaa agaaaaaact
      961 tccttgtagc catgtttctg ataaaagtcc ggtgcctgga actgataagt attgacaaag
     1021 gcaaaacaac aatttcgatt cttagcttca ctttctgcct gttgcaatag ttttgaaccg
     1081 attccttgcc ctcgcagttc ctcttttaca aacaaatact cgatttctag ccaatttcca
     1141 aaagtctctg ctatcaaacc cgcaaggaga ttgccctttt catcttcgac ataaagatta
     1201 agtggctcac tttcagcctc ttcccttttt gaacggttat aaacacgaat cagattccct
     1261 atttcttgcg atttatgtga ttccttattt tccaatctaa agtataatga aatgaaataa
     1321 aacatgcgca aatcgattaa ggaatttaat ctaatttcta ataatgtctt agaaatcaaa
     1381 gtttactatt ttaacttcaa tgcactatac atctaatact caatgaaaat caaagagcaa
     1441 actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgtaga ta
//
LOCUS       DAVHIT010000154         1474 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVHIT010000154 DAVHIT010000000
VERSION     DAVHIT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.156"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            252..592
                     /gene="tnpB"
                     /locus_tag="VVN16_002029"
                     /pseudo
     CDS             252..592
                     /gene="tnpB"
                     /locus_tag="VVN16_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(625..1474)
                     /locus_tag="VVN16_002030"
                     /pseudo
     CDS             complement(625..1474)
                     /locus_tag="VVN16_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          442 a          302 c          271 g          459 t
ORIGIN      
        1 ttaatttcat aataaaaaca ccccaaaaag ttagattttt tctgtctaac tttttggggg
       61 gcagtacact acacctacta ttatacgttt ttatcatttt gaaatctact gctaatcaat
      121 gaagaagcaa agatcaaact aggcagctag ttacaagtta cgtaagcgcg tcataacaag
      181 gtatctatcc taattcccca acttccagta tactagataa aaaataatta tcggaggaaa
      241 ggtattgtac tatgataatt caactaagtg atttaggtca agttcacctt gtttgtagca
      301 agatagatat gaggcaggga atagactcat tagcctatgt agttaaaacc cactttgaat
      361 tggatccttt ctccggtcaa gtctttctct tttgtggtgg acgtaaagac cgctttaaag
      421 ccctttactg ggatggtcaa ggattttggc tactatataa acgctttgag aacggcagac
      481 tgacttggcc cagtacagaa aaggatgtca aagctctcac acctgaacaa gtagactggc
      541 ttatgaaggg cttttctatc actccaaaaa taaatccatc agaaagtcgt gatttctatt
      601 gaaatgagga ctttcttttt atactcatct gctttcaaaa agcattctag tccatctccg
      661 attaacgatg ggctttatca cctccttctc cagtccttgt atgatatctt aaagttgatt
      721 catgacatct tccaaagttt gaaaggcttt atttttaaat ccacgtttac gaatctcttt
      781 ccacacttgt tcaatggggt tcatctctgg tgtgtacgga ggaataaatg caaagccaat
      841 attagtcgga atctttaagg tacttgattt atgccatata gcattgtcca taacgaataa
      901 aagataatca tctggataag cttgtgaaag ctcttctaaa aagacgttca tccactcagt
      961 attacatcca ccagctatta agaaaaatga ttcgcctgta tgggtccata acaatagcga
     1021 aattctcgta tatagtgact atggacatgt ggacctactc ctattggaga ccaacaagat
     1081 cccagtttac tgattctacc gaagccagcc tcatcttggt acatcaagcg aaccttatga
     1141 aaacgtctac tggttttaaa acgctttctt ttcttcttga attgagattt tatttttaga
     1201 cgcgacaatg gtttgagcgt ctgctttctt aggatgttct ggacgtggca taatatttcg
     1261 ccaaccatgg cgcttcaaca gttgatagaa ggcatcacgt gtgtaggaac gacctaactc
     1321 ctttttataa gcctgaaata aggcatcaat tgtaacaaat tctcctgcct ctgcagcctt
     1381 caaatgacgg gcaagaaagg ctttctcttc cccaaccgtc atatatgcat ggttacgacc
     1441 accacgtgtt tcttgaagga gagagtcgag tccg
//
LOCUS       DAVHIT010000155         1440 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVHIT010000155 DAVHIT010000000
VERSION     DAVHIT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.157"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..416)
                     /locus_tag="VVN16_002031"
     CDS             complement(<1..416)
                     /locus_tag="VVN16_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5573326.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
     gene            538..1284
                     /locus_tag="VVN16_002032"
     CDS             538..1284
                     /locus_tag="VVN16_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5573327.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1297..>1440
                     /locus_tag="VVN16_002033"
     CDS             1297..>1440
                     /locus_tag="VVN16_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU5573328.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAG"
BASE COUNT          520 a          190 c          271 g          459 t
ORIGIN      
        1 aaatccacat attgaattaa atcgataaat ttttcatgat caacaaaggc agtagtttca
       61 atggcagtgt gtatgtgatg ttctttagct gattttaaga tggctttagc aaattcaaac
      121 tgagcaaata tttcacctcc tgataaagtt aaacctccac cggattcttc gtaaaattct
      181 ttgtctttta atacctctgt aataatttct tctacagtct tttcttcacc gactaaggtg
      241 aatttctctc gttgagcatc tttcattttt tcaggtttca ttctttgaga ttcaggatta
      301 gaacaccatg gacagcgcag aggacatcct tttaaaaaaa cagttgtacg aatacccggg
      361 ccgtcatgaa ttgaaaagtg ttgaatatta aaaataattc ctttagatat ttccataaga
      421 gttcctcctc ttcataagtc tatcctatca caaaaacgaa agaaaaacaa ttacgaacga
      481 aaggttaatt tgttgttttt tgctttttat tttggtagaa taaaacggag gtgtaaaatg
      541 aaaagattgg aacaaattat taaattagta tcagaatatg aaaagatcga cgttaataca
      601 ttatcggaaa aattaaatgt atcgaaagta acgattagaa aagatttaga taaattagag
      661 tcaaaaggtt tattacacag agagcatgga tatgctgtat taaatagtgg agatgactta
      721 aatgtacgtt tgtcaattaa ttatgaaatt aagagaaaaa ttgttcagga agcagtaaaa
      781 ttggtgtcag ataatgaaac aataatgata gaatctggat cgacctgtgc tttacttgct
      841 gaggaaattt gcaagcaaaa aagaaatgtt acgattgtaa caaattcatt ttttatagca
      901 aattttgtga gagcttatga ttcatgtcgt attattgttc ttggtggaga gtttcagaag
      961 gattcacagg ttactgtggg acctttatta aaagaaatga tacagacttt tcatgtgcat
     1021 caagcttttg ttgggacaga tggctacgat aaagagatgg gctttacagg aaaagattta
     1081 atgcgcagtg aggtagttca atatatttca gcagcgtcgg ataaagtcat tgtactaact
     1141 gactcaagta aatttgataa aaggggtaca gtaagaagat ttgctttaag tcaagtctat
     1201 gaagtaataa cagacgaaaa actttctaaa caaaatatag ctacattaga aaatgctggg
     1261 ataatggtta aggtagtttc gtaagaggtt aagtgtatga atcaagatag gaataaactg
     1321 ctctctaaaa ttgcttatct gtattatatt gaaaacttaa atcagtcaca aatagcagca
     1381 aaattaggaa tttatagaac ctctattagt agaatgttaa cagaagcaag gaatgcagga
//
LOCUS       DAVHIT010000156         1434 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVHIT010000156 DAVHIT010000000
VERSION     DAVHIT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.158"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            76..1254
                     /locus_tag="VVN16_002034"
     CDS             76..1254
                     /locus_tag="VVN16_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU5573329.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NELIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          528 a          262 c          210 g          434 t
ORIGIN      
        1 agattaacat ctacatttat ccatagcaaa aaatcatagg attttaagaa tctgacaact
       61 aggaagaaaa ctaatatgac cttatctaag aaacaattac aactacgagc taaaatactg
      121 gaaacagttt acacattagg tcccatttca cgaattgaaa tcgctactaa aacaggaata
      181 acacctgcaa caactagcag tatcacaaat gagctgatta aagaaaacat tctcttagaa
      241 cttggtgaag acgaacacga tacaagcgta gggagaaaaa aaatcctgct agacattcaa
      301 gctaaacgat tttactacat tggttgtgaa ctatctgaaa aacatttcac ttttgcttta
      361 ggagataatc taggaaatat cttaaaggaa gagaaagaaa ttgttactaa acagttaatc
      421 caagaaaagg gaaatcaact cattaaccag acactaaagc aattcttaaa taactgctct
      481 gattatgaaa tagaagcaat tggaatcgcg ctacctggtc gctatttaga tgattataaa
      541 ataacaacaa acaatccttt atggcaacat atcgacttag aaatgattca atcccacttt
      601 gataagcctt tgtttttctc taacaatgtt aactgtatgg ctataggtaa acgcctattt
      661 agtcgccaac agaatgaccc taattttgct tattttcatt ttgctagagg tatgcactgt
      721 tcttatatct acgacggaaa catttatggg aaaggaaatc taatgattgg agaaataggg
      781 catacagttg tttcatctga gggagaagaa tgtagctgcg ggcgcaaggg atgcttacaa
      841 acttttgctg gagaatcttg gttaatcaaa aaatcaaaaa ttctatatca tcaatctccc
      901 tattctctac ttcctagttt agtcaaaaat gccgatgata ttgatatcca agttatttta
      961 accgcttatc aattaggtga cactggaata atcacactta tccatcaagc cttactctat
     1021 cttagtcaaa ccattttaaa tatcagtatg atgattgact ctcaaaaaat ctacctacat
     1081 agtcctttac taacaaatca acacatcatc caaaaactat atagtgaaat gaactacaaa
     1141 cccaaactgc tctataatcg cctacctgaa gtcattattg aaccttacaa tgattttaca
     1201 gccgcacact cagctattgc actttgtttg tatcatacaa ttctacattc ttaattttct
     1261 attacattta ataaataggc taaaaagagg gagtatatca taaaaagcga ataaacctgt
     1321 tttctgaaaa tcagaattct gttttgttcg ctttctttat atttggaatt atactcctat
     1381 ctcccatttt tcataatgta tagtaaaatg aaataataac aggacgaatc gatc
//
LOCUS       DAVHIT010000157         1429 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVHIT010000157 DAVHIT010000000
VERSION     DAVHIT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.159"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..705
                     /locus_tag="VVN16_002035"
     CDS             <1..705
                     /locus_tag="VVN16_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573330.1"
                     /translation="YHQFLLGNNKTLQVLKNVSLSASRGEFISILGISGSGKSTLLKC
                     ISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIPSLPVIENIALPL
                     RLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAVIADSDIIFADEP
                     TGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILKDGKIFKELHRPS
                     GEELYKILEVQSTTEE"
     gene            708..>1429
                     /locus_tag="VVN16_002036"
     CDS             708..>1429
                     /locus_tag="VVN16_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5573331.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLS"
BASE COUNT          458 a          190 c          278 g          503 t
ORIGIN      
        1 tatcatcaat ttttactagg aaataataaa acgttgcaag tgctgaaaaa tgtttctctt
       61 tctgcttcga gaggagaatt tataagtatt ctaggaatta gtggttctgg aaagtcaact
      121 ttattaaaat gtatttctag tttgcttgaa ccaacaagtg gggaagtaat tttaaatgga
      181 atcaacccct ataaaatcag aaatgcaaaa ttgtcaagta taagacgtaa cgaagtatct
      241 tttatatttc aagcatacaa tttaatacct tccctgccgg taatagaaaa tatagcactt
      301 cctttgcgat tatcacaaaa aaaattaact attaaaaatg tagaaaactt actcaaaaga
      361 atgaagttta atgctggctt aaacgatttt gttggaactc tgtctggtgg agaacaacag
      421 aaggttgcta tagctagagc ggttattgct gatagtgata taatatttgc tgatgagcca
      481 actggggctt tagacagcgt ttctcgtgaa gtaatttttg aattattgag agagttagta
      541 ggggcgggta agtgtgtaat tatggtaacg cacgatatag aattggcctc gaaaactgat
      601 cgtgcattaa tattgaaaga tggaaaaatt ttcaaagaac ttcatagacc tagcggggaa
      661 gagttgtata aaatcttaga ggtacaatca actacggagg aatagagatg ctcagattag
      721 tttactatca atttttacat aataagaaac aatggttagg cgtttcacca gtaatttttg
      781 tttcgagttt agttatggga ttggctgtta atggggtaat taatgttgag aataattcgc
      841 aagtatttgt cggacttcca gatcctaaac caatttttat gtttccgata gtattcggag
      901 gagtaacatt attctttgtg ctctctaaca taataaatat gttggttgaa atttttagag
      961 atgattacga acttctggag gtgttgggag caagtcgcct tcaactatca tttcttgtgg
     1021 ggggacagat ttttatcatt tcctcgatta ttagttttat tgcatatcta tgctctatat
     1081 ttgttacgtc aaattattat tattttttac aatacttttt cggggaaaat atattacctg
     1141 atatccaatt tcaaacgagt gctgttggat gtatcattac agtagtgttg atttcatttt
     1201 tagcctttct aagtggttgc ttttatacgt tcaagaagat tagaaatagg aagagttcta
     1261 aaataaggca tgttctttca atagtaaaaa gaatactgct attggctggt tttagtgtta
     1321 tctggttgct ttctttgcaa caaattttcc aagatagcac aattttagcg aaggcccaga
     1381 taatattcaa tattgtaatc ttagatattg tgattattta tcaattatc
//
LOCUS       DAVHIT010000158         1415 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVHIT010000158 DAVHIT010000000
VERSION     DAVHIT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.160"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..1402)
                     /locus_tag="VVN16_002037"
     CDS             complement(<1..1402)
                     /locus_tag="VVN16_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU5573332.1"
                     /translation="MKSFQQNEVFSIRKSKVGVCSVLLAVLFIGTQSVLANEVVGETS
                     SSKDNMQMNSLLSSTSTSLSAEPHSDTLSLDNNSSLSTDNNGGVSINQNKPDITNDTM
                     MGNISGMEVNTILSTNLNEHVALNVKDYGAIGDGVNDDRQAIQDAIDAAAQGLGGGNV
                     YFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIVFMTGLFTDDGAQVEWG
                     PTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNSNNVTIKNVTFKDSYQG
                     HAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEPLTRKGFPYALNDDGKK
                     SENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKILNNHFDNMMYAGVRFTG
                     FTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLLDLNKQVVIAENIFNIA
                     DPKTKAIRVAKDSAEYL"
BASE COUNT          430 a          282 c          196 g          507 t
ORIGIN      
        1 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
       61 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
      121 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
      181 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
      241 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
      301 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
      361 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
      421 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
      481 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
      541 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
      601 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
      661 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatagtac
      721 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
      781 ataaacctgt cataaaaaca atagaagggt gattcttaat atttatacca tttagaatgg
      841 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
      901 aataagttcc ttccggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
      961 ttgcatcttg aattgcttgt cgatcatcat taaccccgtc acctattgct ccataatctt
     1021 taacattcaa agctacgtgt tcgttcagat tagtacttaa aatagtattc acttccatcc
     1081 cagagatgtt acccatcata gtatcatttg ttatatcagg tttattttga ttaatactca
     1141 ctcctccatt attatcagta cttaaacttg aattgttatc aagactgaga gtatcagaat
     1201 ggggttccgc agataatgat gtagatgtgc tactcaacaa agagttcatt tgcatattat
     1261 ctttagacga agaagtttca ccaactactt cattagctaa aacactttgt gttccaataa
     1321 ataaaaccgc taagagaact gagcaaactc ccaccttaga cttacgaata gagaaaacct
     1381 cattttgttg aaaagatttc atattagcct ccaat
//
LOCUS       DAVHIT010000159         1400 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVHIT010000159 DAVHIT010000000
VERSION     DAVHIT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.161"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..970
                     /locus_tag="VVN16_002038"
                     /pseudo
     CDS             1..970
                     /locus_tag="VVN16_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1149..>1400)
                     /locus_tag="VVN16_002039"
     CDS             complement(1149..>1400)
                     /locus_tag="VVN16_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5573333.1"
                     /translation="VPAQPGTEDKKPSAPKPDMQPSPQPEGKKPSVPEINQEKEKAKL
                     AVATEKKLPSTGVASNLVLEIIGLLGLIGTSFIAMKRRK"
BASE COUNT          485 a          252 c          259 g          404 t
ORIGIN      
        1 gagacctgaa gagagaacag aattagaacg tcttcaagca gaaaatgagt acctgagagc
       61 ggagaatgcc atcctaaaaa agttaagaga actccgattg aaggaggaaa aagagaaaga
      121 agaaagacag aaattgttca agaattaatg actgagtttt cgttagatct tcttctaaaa
      181 gccattaaac tagctcgttc gacctactac tatcacttga aacagctaga caaaccagat
      241 aaggaccaag agcttaaagc tgaaattcaa tccattttta tcaaacacaa gggaaattat
      301 gcttatcgcc gggttcattt agaactaaga aatcgtggtt atctggtaaa tcataaaaga
      361 gttcaaggct tgatgaaagt actcaattta caagctaaaa tgcgacagaa acgaaaataa
      421 aggagacgtt ggtaagaaag cagagaatct cattcaaggc caatttgaag gctctaaaac
      481 aatggaaaag tgctacacag atgtgacaga atttgccatt ccagcaagta ctcaaaagct
      541 ttacttatca ccagttttag atggctttaa tagcgaaatt atcgcctata atctttcaac
      601 ttcacccaat ttagaacaag tacaaacaat gttggaacag gcattcacag agaagcacta
      661 cgagaatacg attctccata gtgaccaagg ctggcaatat caacacgatt cttatcatca
      721 gtttcttgag ggtaagggaa ttcaagcatc catttcacgt aagggtaaca gcccagacaa
      781 tggtatgatg gagtcctttt ttgggattct gaaatcggaa atgttttatg gctatgagaa
      841 aacatttaaa tcacttaatc gattggaaca agctattgta gactatattg attactacaa
      901 caataaacga attaaggtaa aactaaaagg acttagccct gtgcaataca gaactaaatc
      961 cttcggataa attaattgtc taacttttgc ggttcactag ccttctcttt ttatatctta
     1021 gattcatctc tatatgaaat gacttataat tattagatac caattataag taaaatcttt
     1081 gggatcttca atggttaacc aatcaaatgc taaatatcga aagtggatat ttttaaaaaa
     1141 ctgaatcatt attttcttct tttcatcgcg atgaatgaag ttccaatcaa accaaggaga
     1201 ccaatgatct caagaactag attagacgcc actcctgtag atggcaattt cttctctgtt
     1261 gctacagcaa gcttagcttt ttctttctcc tgattaatct ctggtacact tggtttctta
     1321 ccctctggtt gagggcttgg ttgcatatct ggttttggag cagatggctt tttatcctca
     1381 gtaccaggtt gtgccggtac
//
LOCUS       DAVHIT010000160         1362 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVHIT010000160 DAVHIT010000000
VERSION     DAVHIT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.162"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..212)
                     /locus_tag="VVN16_002040"
     CDS             complement(<1..212)
                     /locus_tag="VVN16_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573334.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTISVIQTGVFILED"
BASE COUNT          485 a          251 c          182 g          444 t
ORIGIN      
        1 aaatcttcta aaataaaaac accagtttga ataacagata tcgtatgact agcaaaatct
       61 cgtgtgattt tgtgtaagcc aaataaatga tgtaaatcag tcaagcaaaa attcactttg
      121 aaagtctcta aatacttata attcgttgat acttgacatg tttttccatt gaatctttct
      181 tgataatcca aaatacaatc aagtaatttc aacgacaaac tccatatttt ctcaaaataa
      241 aaagcacggg gcagttctca ctactgccca aagtgattga ttagagtcgg cttctctaac
      301 tccctatgtt gtgcttatta ggcttatcgc aagggaacag tttttagctc tgccaagcca
      361 cataatgtgc ttactactat gtgtccatgt caccatgaat tgccgaacgg tttaacgaga
      421 aagtatagta gattgaaaca agatgtgaac aaatcgatta ggaaagtcaa attaatttct
      481 agaaatattt taacagtcat aacggactat tccagattca atctactata ttacttttag
      541 gtttatccca actcaattat gacatctttt tttcaaaagt caatatatct cactcttttc
      601 aacgacaaga aagaggctga taatcgacca acctctgttt ttagatacag ttcaagtcca
      661 aactattctt tatagaaaag tccgataaat tcgagcaata gttaaaatac aaacaaatat
      721 aattcccaaa atagataaca ctattttcac aaaattcatt tcagaaccta tgacatatat
      781 atgaaaaaga ggggctagtc ctacacatat atacaataag tagccaacaa gttttatctc
      841 accaattcct gcaaaaaaat gtttcatgtg acgattctcc atttactcca ttaaatcagt
      901 tttattttag actatcataa aacaatcacc tcttcaataa aaaccatttg cgaaatacta
      961 ttctgaagca ttgataggtc agatttctca gaatagagct gtcctaatta tcgagcagct
     1021 caaatacata tatatgagca attctaagcc tagaattgtt ataaaataaa tgttttcagg
     1081 ctattctaag tctttgactg cttaaaattt tacgttctga aggattctaa agctagaata
     1141 gctcaaaatc acgagaacta aggtattcaa aacttcagag taatacaatc cccataggaa
     1201 ttctccattc ttattaagca tgattaaaat gtaatcctaa caagtgatcc tcgactgtgt
     1261 ttgtagcact tagttaccta gataagacta tttcccttta tcatatctga ttttaagccg
     1321 ttctaagatt taacctatgt ttctattact tgctattttt aa
//
LOCUS       DAVHIT010000161         1353 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVHIT010000161 DAVHIT010000000
VERSION     DAVHIT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.163"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(473..610)
                     /locus_tag="VVN16_002041"
     CDS             complement(473..610)
                     /locus_tag="VVN16_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU5573335.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            complement(812..1333)
                     /locus_tag="VVN16_002042"
     CDS             complement(812..1333)
                     /locus_tag="VVN16_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5573336.1"
                     /translation="MKFLCIGELTILQILYGSSLISFLWMYGQRKQAHKVNMKSRMKW
                     LGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQVPWFSFLLFLINASMVEEFLYRE
                     ILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYVSLGMFLGLVRYKLDLWGSMGLH
                     LVWSLLVYSLLLF"
BASE COUNT          487 a          289 c          185 g          392 t
ORIGIN      
        1 ggggtgaata attggggttt aaaattgtct aaattcgaca gaaggaagta gcaagcattg
       61 tcgctaaaaa aacgcatcag gttatcctgt gcattttttt gatatcaaaa taatcaatct
      121 cctttatgag ctaattcttc tctacaagct ctttggagca gaacaactgt aagtataaaa
      181 accacacata aaatcaagat acttctctca gccccaaaaa caccactagt atataaatct
      241 tgacccgtta aatttgtact gaataaacta aatgaaactt ttatcccact cactaatact
      301 attatatcaa cccactacag ttgacaaaaa gacaaaccag cctaatgact agcttgtctt
      361 caatcaagag aggattctgt tccccccata taagcactaa cattccaacg tgcacatatt
      421 ggaacgacat ccataactcc agagaatctc taaagtttac aatttaaatg aattaacaat
      481 tttcccaact aaaagcactc cagttaccgc aacggtttgt actgaatgta ctaaatcgca
      541 ttccatcaac ttcatctgtt tcgtcaactt gaacagatac taattgaaga tttaatactt
      601 cttctgccat agctaactcc tcctatttaa atttttggga ttaagtgctt tatccacctt
      661 cattatactc tctccagcag taaaatgcaa gcaatgatac aatgttgtca cgtagaaaat
      721 aatgtttccg taacttttca aagtaacttc catctctctc ccaaaactgg aagttagttt
      781 tagaagttac ctaaaaatca ggttacctat tttaaaaaag cagcaaacta taaactagta
      841 ggctccacac caaatgtagc cccatactgc cccataagtc caatttgtag cgtacaagcc
      901 ctaaaaacat ccccagcgat acatacaaac accaagctat aatggttcct ggatgatgtg
      961 ctaaggcaaa taaaacactt gtcaaagcaa ctcgaatatc taattttcta accaagttcc
     1021 ataaaatttc tctatacaga aattcttcaa ccatactcgc attgattaaa aacaataaaa
     1081 atgaaaacca aggaacttga tgttgaagtc caattaagtt tgcttgattc gtgctttctt
     1141 gcgcatggat taggctaaaa catagactta taatcagtag gctaacgaat ccaataccaa
     1201 gccatttcat cctagatttc atatttacct tatgagcttg tttgcgttgg ccatacatcc
     1261 ataaaaaaga aatgagtgac gaaccataga gaatctgtag tatagttaac tcaccgatac
     1321 aaagaaattt caataagtat agagatacca ata
//
LOCUS       DAVHIT010000162         1333 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVHIT010000162 DAVHIT010000000
VERSION     DAVHIT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.164"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            143..835
                     /locus_tag="VVN16_002043"
     CDS             143..835
                     /locus_tag="VVN16_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5573337.1"
                     /translation="MNTSFVHAVDGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVIKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTRTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGSTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            889..1278
                     /locus_tag="VVN16_002044"
     CDS             889..1278
                     /locus_tag="VVN16_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU5573338.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT          487 a          194 c          300 g          352 t
ORIGIN      
        1 tttaacgttt gatagaaaag atgctatact agaagtagga tgtaatggca aaatctaata
       61 ctaataagcg ctatgcatta cgtaaactcg gaaagatttt tggttctgcg gtaatcgggc
      121 taactttatt tttaggagga atctgaatac aagttttgtt catgctgttg atgggattca
      181 atatgtcaga gatgatacta gagataaaga agagggaata gagtatgatg acgctgacaa
      241 tggggatatt attgtaaaag tagcgactaa acctaaggta gtaatcaaga aaatttcaag
      301 tacgcgaatt cgttatgaaa aagatgaaac aaaagaccgt agtgaaaatc ctgttacaat
      361 tgatggagag gatggctatg taactaggac aaggacctac gatgttaatc cagagactgg
      421 tcatgttacc gaacaggtta ctgttgatag aaaagaagcc acggatacag ttatcaaagt
      481 tccagctaaa agcaaggttg aagaagttct tgttccatgt gctactaaat atgaagcaga
      541 caatgacctt tctgcaggac aggagcaaga gattactcta ggaaagaatg ggaaaacagt
      601 tacaacgata acttatgatg tagatggaaa gagtggacaa gtaactgaga gtactttaag
      661 tcaaaaagaa gactctcaaa caagagttgt taaaaaagga accaagcccc aagttcttgt
      721 ccaagaaatt ccaatcgaaa cagaatatct cgatggctca actcttgata aaagtcaaga
      781 agtagaagaa gtaggagaaa ttggtaaatt actcttacta caatctatac tgtagatgaa
      841 cgtgatggaa caattgaaga aactacttct cgtcaaatta ctaaagagat ggtaaaaaga
      901 cgtataagga gagggacgag agaacctgaa aaagttgttg ttcctgagca atcatctatt
      961 ccttcgtatc ctgtatctgt tacatctaac caaggaacag atgtagcagt agaaccagct
     1021 aaagcagttg ctccaacaac aggctggaaa caagaaaatg gtatgtggta tttttataat
     1081 actgatggtt ccatggcaac aggttgggta caagttaatg gttcatggta ctacctcaac
     1141 agcaacggtt ctatgaaagt caatcaatgg ttccaagttg gtggtaaatg gtattatgta
     1201 aatacatcgg gtgagttagc ggtcaataca agtatagatg gctatagagt caatgataat
     1261 ggtgaatggg tgcgttaaca gattcctccc tgcgacaaaa ttgataatag tatgtctgaa
     1321 tatataatta tat
//
LOCUS       DAVHIT010000163         1325 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVHIT010000163 DAVHIT010000000
VERSION     DAVHIT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.165"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..214)
                     /locus_tag="VVN16_002045"
     CDS             complement(<1..214)
                     /locus_tag="VVN16_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573339.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGD"
     gene            complement(228..941)
                     /locus_tag="VVN16_002046"
     CDS             complement(228..941)
                     /locus_tag="VVN16_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU5573340.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          435 a          254 c          215 g          421 t
ORIGIN      
        1 aatccccttc tttgactaat gatggagatt tccaagtaaa aattaaatcg ccagagaaat
       61 ttttcttatc aaaaaactta aaaagagatt gataaatact atttgctaat tgacaagtgt
      121 cagatgtaaa ttcataatta acagcttcat taaaatcaac ttcagacatt tcaatgtact
      181 cttcaatagt aatattagct ttttttgtag tcattagcta atctccttta aaacgtgatc
      241 ttttcaagaa tatttttatc taaacaatcc agcaagtctt ggtaagaata gacttcgtaa
      301 gtcggctggg cttgtgtgtg attttcgagg tgatgaggat tataccagat agtgtcaatc
      361 cccgcattat tgccaccttg aatgtcggcg gttagagaat ctccaatcat cagcgtcttt
      421 tctttactaa atccagcaat ttgctggcca atcttttcat aaaaaagagc atccggcttt
      481 tgagtttgca actgttctga gataaagact tgattgaaat aaggtgctag accagattga
      541 gccaaacgtc ctgtctgaat ggcagtaatg ccatttgtcg cagcatacaa gttataatca
      601 cgctcaatga ggctgtccaa gagatcatga gcgcccgata gtgtttgtcc ctgctgggcg
      661 aggtaaaatt ggtaacgctg ggcaagaaaa ctaccgtctt tttcctgtcc aaaatgagca
      721 aataaacgag aaaagcgcgt gttaaccagc tcttgtttac tgattttctt cagctccaag
      781 tctttccaga gagccttgtt cataggaacg taataatctt tataagcctg aatatccgca
      841 actccttctt cttttagaag ttgggtcaaa gccacatcct cagcagcatc aaaatcaaga
      901 agagtgtggt cgaggtcgaa gagtagaaat ttgtagaaca atttgaggtt ttcctttctg
      961 aaaattcatt aagaacatta tatcataaag cacctcatac aattaactaa tttaatcact
     1021 taaaaaaaat tcgaacactt tctatacaac tgacagctca aatctttcag aatagaacaa
     1081 tactaactat ccagcacccc atcttcataa atatatgtgt aattctagac ctagaattcc
     1141 tataaactaa atgctttcat actcttccaa gtaattgatt tccttaaatt ttaatttttg
     1201 aaggattcta aagctagaat agccccatca caatcagttt tgattgattc acaatttaga
     1261 aacactatag tttcactcct gttaaaataa aaaggaattg cataaagcaa tccctttctg
     1321 atttt
//
LOCUS       DAVHIT010000164         1310 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVHIT010000164 DAVHIT010000000
VERSION     DAVHIT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.166"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            85..435
                     /locus_tag="VVN16_002047"
     CDS             85..435
                     /locus_tag="VVN16_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU5573341.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSNIHVLLSMPYSVQDFYTNIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            758..991
                     /locus_tag="VVN16_002048"
     CDS             758..991
                     /locus_tag="VVN16_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573342.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            1118..>1310
                     /locus_tag="VVN16_002049"
     CDS             1118..>1310
                     /locus_tag="VVN16_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5573343.1"
                     /translation="MEQLHFITKLLDIKDPNVQILNIINKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYL"
BASE COUNT          432 a          262 c          265 g          351 t
ORIGIN      
        1 aaatatttct ataaattaat ttgactttcc tgatagagtt attcacatct tatttcaact
       61 cactatagaa ggaggaatag ggggattctc agacatccgg gcatcagccc aactaatgat
      121 ttgattgcta agaaaatatt cagcaatcca gaaatcactt gtcaatttat tcgcgatatg
      181 ctggatttgc cagccaaaaa tgtgaccatt ttggagggta gcaatattca cgtattactc
      241 tccatgcctt actcggtgca ggatttttat accaatatag acgtcttggc ggagttggat
      301 aacggtactc aagtaattat tgagattcaa gtccatcatc agaatttttt catcaatcac
      361 ttgtgggctt acctgtgcag tcaggttaat caaaatcttg aaaaaattcg tcagcgagaa
      421 ggtgatactc actagagcta caaacacatc gctcctgttt acgccattgc tatcgtggat
      481 agtaattatt tctcagatga cctggctttt catagcttta gtatgcgcga agacacaaca
      541 ggtgaggtat tggcgattac caacaatgga caggaaaacc atctggttaa gatggcattc
      601 ttggaattaa aaaatacaga gaaaccagca aagacaaggt tcgcaagcca tggttggagt
      661 ttttcgggaa caagcccttt acccagcaac cgcaacgagc cattacccaa gcaaatcaac
      721 tgctggacta caagagctgg tccgagggga caggaaaatg tttagtcaac tacatatgcg
      781 agaagaacaa gtcttgttag cacaggacta tgccttggaa accgctaggg ctgaaggcct
      841 tgaacaagga ctagagcgtg ggaaagttga aggaagggca gaaaggaaac tttttgcctt
      901 cctagacata gtacgccaag gtcttctgac ttctgaggtt gccagccagc aattaggtat
      961 gtcagtatct gaatttgagg cactgttgta aaatggctcc ataatatcca tagtgggtaa
     1021 atcccctatg gatattatgg agcctatttt gtgtagaaaa aaagtcccat atgacctata
     1081 atgaaaagcg acaaaacaac tcattagaaa gaatcatatg gaacaattac attttatcac
     1141 aaaattacta gacattaaag accctaatgt ccagatttta aacatcatca ataaggatac
     1201 acacaaggaa atcatcgcca aactggacta cgacgcccca tcttgccctg agtgcggaaa
     1261 ccaattgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg
//
LOCUS       DAVHIT010000165         1308 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVHIT010000165 DAVHIT010000000
VERSION     DAVHIT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.167"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..559
                     /locus_tag="VVN16_002050"
                     /pseudo
     CDS             1..559
                     /locus_tag="VVN16_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(594..1308)
                     /locus_tag="VVN16_002051"
                     /pseudo
     CDS             complement(594..1308)
                     /locus_tag="VVN16_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          407 a          217 c          271 g          413 t
ORIGIN      
        1 gctatgggct acactcgaaa aaaagaacca cacctactat gaacaagacc cagaaaaagt
       61 agccttattt cttaagaatt ttaatagttt aaagcaccta gcacctgttt agattgacga
      121 aacaggattc gatacttatt tttatcgaga atatggtcgc tcattaaaag gtcagttaag
      181 aagaggcaaa gtatctggaa gaagatatca gaggatttct ttggttgcag gtctaacaaa
      241 tggtgaatta atcgctccaa tgacttacga agagacgatg acgagcgact tttttgaagt
      301 atggtttcag aaatttctct taccaacatt aaccacacca tcggttatta ttatggataa
      361 tgcaagattc catagaatgg gtaagctaga actcttgtgt gaagagtttg ggcataaact
      421 tttacctctt cctccctact cacctgagta caatcctatt gagaaaacat gggttcatat
      481 caaaaagaac ctcaaaaagg tattaccaag ttgcaatacc ttttatgagg ctcttttgtc
      541 ttgttcttgt ttcaattgac tatattagag gcgagacatt tttcggttct ttgtcaactg
      601 tagtgggttg aagaaagcga agatctagaa aggacaaatt tcgtcctttc ttttttgaag
      661 ttttcaaagt tcctaaaacc aaaggcattg tgcttgataa gtttgatgag attattggtg
      721 gcttccagtt tggcgttgga ataaggtaat tgaagggcgt tgacgatttt ctctttatct
      781 ttgaggaagg ttttaaacaa agtctgaaac agaggtggaa aagcaagagc tgatagagat
      841 tatagtggtg tttaaagtct tcggaatagc tcaaaagttt atctagaatt tctttattag
      901 tcaagtgcat acgaaaagta gggcgataaa atcgtttatc actcagtttc tgactatctt
      961 gttgaatgag cttccagtag cgcttgatag ccttgtattc atgggatttc ggatgatggc
     1021 ttgtgttctg ctctcaagaa cagttatgat attgagttta tcaaagtcct gagcaataaa
     1081 gctcatctcc atctcccgat tgaaacagtc actccccgga ctgtttcaac gtcctaggac
     1141 ataatctcag gaagacgcga aaaatcatgc tcaaagtgaa aatcattgat cttgcgaatg
     1201 acagttgaag ttgaaataga caactgatga tcaatgtcgg tcatagaaat cttttcaatt
     1261 aacttttgag caatcttttg gttgataata cgagggattt ggtgattc
//
LOCUS       DAVHIT010000166         1295 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVHIT010000166 DAVHIT010000000
VERSION     DAVHIT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.168"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            84..>1295
                     /locus_tag="VVN16_002052"
     CDS             84..>1295
                     /locus_tag="VVN16_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU5573344.1"
                     /translation="MNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPN
                     ELHKLIEKIAPKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGD
                     FSKKNIVLKEDDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNT
                     FKLDAKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLE
                     VLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQ
                     LRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKA
                     KFRELANIAFRQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQILN
                     SVTGDEKKIIEF"
BASE COUNT          485 a          153 c          238 g          419 t
ORIGIN      
        1 tagttttgtt tgttgatgca ggaatatcag ctaattctaa tctaccaact tggggagaat
       61 tgattcaatc attaaaaaaa gaattgaaca taccagaaga aagaacagat agtccactga
      121 gaattgctca gtattattat gatacttttg ggaaaaatca gtacacaaag aagatagaag
      181 aaattttctt taagaaggga ttgagtaaac caaatgagtt acataaatta atagaaaaaa
      241 ttgcaccaaa acatattatt acgacaaact atgattcact tctggagagt caatttgaaa
      301 gtggcttgtt aaagtacaat gtagtcgctg aggataaaga catcccttat acgagttcgg
      361 aaagatactt gattaaaatg catggtgact tcagtaagaa aaatattgtt ttgaaagaag
      421 atgattattt agactatcat ttgaacttcc caatgatttc tactttaatc cagtcattga
      481 taatgaatca tactctattg tttgtggggt attctctaag tgattcgact tttaattcaa
      541 tttttagaat gattcaaaat acatttaaat tggatgctaa aaatgcttat ttttatactc
      601 ccgaggaacc gtctatgatc attcgagagt attataagaa gcaaggaatt ttcattatct
      661 ctaatgaaga gaatctaggt caggagacat cagaaaaaca gaacaaattg tattgcagga
      721 caaaagattt tttagaggtt ctatcagaaa accgaagtca agatgtaaat aatgctgatg
      781 atttatggaa tcaactggct tttttagata gacttagttt tattgatgcg aaagattttt
      841 ctagatattc tgatttaaag aaaagggctt taaactggga tgatgagtat tgctggtttg
      901 gaaatgatca attaagattt gaaattgatg gtcatgaaga attacgaatt atggtatcta
      961 aaaaatcttt attaaatcgc tttctagata tggagattgg tgagcctagg gatttaaagg
     1021 ggaatcgatt cttaagtaag gcttttaagt tgtatgaaga aaaacaatat tctttagcaa
     1081 aagcaaaatt tagagaacta gctaatatag catttcgtca gaaagactat tttaattttt
     1141 tagtttgtga atttaatttt cagcaaattc aaataattga tagatacgaa aaaacaccta
     1201 gttatgcaga gccactttat gatggagaat taagcgaatt aacagaacaa attcttaact
     1261 cggttacagg agatgagaaa aagattatag aattt
//
LOCUS       DAVHIT010000167         1224 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVHIT010000167 DAVHIT010000000
VERSION     DAVHIT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.169"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(5..664)
                     /locus_tag="VVN16_002053"
     CDS             complement(5..664)
                     /locus_tag="VVN16_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU5573345.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            734..1132
                     /locus_tag="VVN16_002054"
     CDS             734..1132
                     /locus_tag="VVN16_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573346.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIV"
BASE COUNT          449 a          220 c          283 g          272 t
ORIGIN      
        1 tttatcagac aagcagtagc ttaccaaaaa taatcaaaca agtagcaagt aagaggaaag
       61 gcacgaaagg aagtctttcc tttttctttt gcaggagaaa ggccaggata cccgtcgcag
      121 aagcaaactg aatcaagatc agtaactccg ttacgctaaa gacgagagca caagaagcta
      181 aaaagaggaa atctcctgcg cccatgcgga tatcgataaa atgagccaaa attccaagag
      241 caaggaagga gaccatgacc agattccagc cagaggaagc tattaggatt aggtggaaag
      301 tcatccagac cagtaaggga tattcctgat ggtgaaagtc gtagataccc aaggtcaaac
      361 cagcggtgat taggacgact tgccccaagg aaagcaattc ccaagagtaa agcagaaaga
      421 ggagtcctaa gcctagttca aagagggcat accagacagg atagcgaact ttgcagtagc
      481 gacagcgaaa gcgattgaag acctgtgaga gaatcggaat caaatctaag ggacgcaagc
      541 gagtctgaca ggaatcgcag tgactggctg aactgataat ggattgctct ggaaaacggt
      601 caatgaccaa accaagaaag gaagcgagaa tgctcccgac gagaaaaaaa taaaaatcaa
      661 tcatacttat ctattcgtaa aaaatgggag aaatagtata atggagaaac atagataaga
      721 tggtgaggaa aagatgacaa ttcgttttga agaaaaggtg agtatagaaa acgctcaatt
      781 tgtatgccag tggtccaact ccctaggcaa atcctttcaa gaacaatgga tgggaccaag
      841 gattcctttt ctactgaccc ttcaagcctt ggaaggagtc ttttcaatct ttgatgaaca
      901 agagtttgtg ggacttatcc agaaaatcag gctagaagac agcaatcttc atatcgggag
      961 attttttatc aacccccaga aacaggagca aggcttaggt agccaggctt taaggaaatt
     1021 tgttagtttg gcctttgaaa atgaagatat agatagtatt tctctaaatg tcttcgaggc
     1081 aaatcaaaga gctcagaatc tttaccaaaa agaaggattt gaaatcgttt aaatggttga
     1141 agcacctgta cgaaaatata gtagattgaa actagaatag tacacctcta cttctaaaac
     1201 attgttagaa atcgatttga ctgt
//
LOCUS       DAVHIT010000168         1191 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVHIT010000168 DAVHIT010000000
VERSION     DAVHIT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.170"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(8..388)
                     /locus_tag="VVN16_002055"
     CDS             complement(8..388)
                     /locus_tag="VVN16_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU5573347.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLVFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
     gene            complement(810..>1191)
                     /locus_tag="VVN16_002056"
     CDS             complement(810..>1191)
                     /locus_tag="VVN16_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU5573348.1"
                     /translation="RNKNGQADTNQTEKPNEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLL
                     FGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          323 a          250 c          187 g          431 t
ORIGIN      
        1 aaattcctta tttattgatt tcaagctccg ctaactgtat ttgaataact gacagttctg
       61 caccagcctg aagaagagca gctgcattat aggcaccttc tacaattgga accctgttga
      121 tgatgatact tttatcactg aaatcagtca ccatttttaa gttcatttta gcagaaccta
      181 ggtcaaaaaa tacaagtaaa gtatctgctg gattttcgga aacaacccta tctacttgat
      241 caaaactcgt tccaattcct ccgccctcgg ttcctcctac ataagtaatc ggaacatctt
      301 tagctacttt actaatcagt tcaacaacac cttccgcaat gtgtttggaa tgtgaaacga
      361 taacaagacc aataccaata ctttccatca aaccacccca gtttctaaaa tagcagtaaa
      421 gagtaatcct gatgagaatg atccaggatc aatatgtcca agaaaccaca tgctcctaag
      481 acaagagcta acagactggc catcaataat agtattgttc tttttttcat cattactcct
      541 taactagtgt ttaactgatt aattagccag taaatagttt atctttattt acactatctg
      601 ttaagatata gtaaaatgaa ataagaacag gacagtcaaa tcgatttcta acaatgtttt
      661 agaagtagag gtatactatt ctagtttcaa tctactatat tttgcacatt ttcatgaaaa
      721 aaatgagaac tagaactcac attctgctct catttttcgt tttcccgttc tcctatcctg
      781 tttttaggag ttagaaaatg ctgctacctt tacttactct cctttaataa agccaatagt
      841 ttttcagctt ctgccataat agtattgttg tcctgggtgc caaatagtaa attatttttt
      901 aatcctgtga gagtctcttt ggcattggac ttgataattg gatcctggat ttttccaagt
      961 aaatcttcag cctctctcag tttttcttca accttttcag tctcgacctg aggttcttct
     1021 gattcctctg gtgattcttc ttctggttcc tctgttggtt ttggagactc tggtttctcg
     1081 ctttgcggtt tctcttctcg aggggtttct tcctcaggtt tttctgtctg aggtttctcc
     1141 tcgtttggtt tctccgtttg attggtatca gcttgaccat ttttgtttct t
//
LOCUS       DAVHIT010000169         1180 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVHIT010000169 DAVHIT010000000
VERSION     DAVHIT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.171"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..495)
                     /locus_tag="VVN16_002057"
     CDS             complement(<1..495)
                     /locus_tag="VVN16_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5573349.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTS"
     gene            complement(455..1135)
                     /locus_tag="VVN16_002058"
     CDS             complement(455..1135)
                     /locus_tag="VVN16_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5573350.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANIIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSRK"
BASE COUNT          379 a          242 c          280 g          279 t
ORIGIN      
        1 tgaagtggtt ttaatcatcc gagtgacaag attgatagcc tgcggtagca gtcttcttaa
       61 gacttgtggg atgatgatgt ggtagtaaag ttgaacatta gtcaagccga gtgcctgtcc
      121 actttcaaac tgatgtttag ggagagaagt gatagctcca cgtaccaagt ctcccatttc
      181 agctgttccc cagagggtaa aaacgataat agctgaagtc tcacctgaga tattgatatt
      241 aaagtttcga gccaagccaa agtaaacgat gaagagtagc accagctggg gcatgatacg
      301 gataaattcc agatacaatc gtgttaaaaa tcgtatgatt ctagaatggg aggtcatgat
      361 gattcccatg actgttccga acatcatgga taagaggaca gacaggatgg atatcccaat
      421 cgtaacgccc aatccctgta agattctcag gagattattt ccttgaaaga gtacttggat
      481 tcccgaatcc tgcatggcgg agcctccttt ctatccagct aaagaccagt gagatgggta
      541 gcagcatgat cagataagca actaccaaca tagctagcgc aatgtctgtc tcatagtaga
      601 gaccaatcaa atccttggcg acatacatga ggtcggccaa agccactgct gagaaaacag
      661 aggtttcctt gataaggaaa atgatattgg cactaaagga ggggagtgcc accgctgttg
      721 cttgcggaag aaccacatag cgaaagacct gtagaggtgt cagaccaata gcgaggccaa
      781 tctcctgctg ggtttgactg atggcttcca gcccacttcg gaaagattct gccatatagg
      841 agcctcctaa aaagacaagc ccaagcgttg cacagacttc tgaagatagg acaatcccga
      901 ttcggggaag accgaagtag agaaagaaga gttgaatcaa aaggggcgta ttacgtgaca
      961 attcaatgta ggctgtcgct acttgcgcca aaacagggat tcgataatgt cggatgatac
     1021 taacgattaa accgagcaga aaggatccta aaattcccca aactgcaata tgcaaggtca
     1081 gaaagaatgc cttttgatat agtggtagat attgttcaac aatggaccaa tccaaaaata
     1141 gaacctccca tctagaaata atacagttat tgtagcactt
//
LOCUS       DAVHIT010000170         1073 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVHIT010000170 DAVHIT010000000
VERSION     DAVHIT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.172"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            33..425
                     /locus_tag="VVN16_002059"
     CDS             33..425
                     /locus_tag="VVN16_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HEU5573351.1"
                     /translation="MVVPRIVVSENYQNPWFREWLEENYKKDLIDHKSYAREWSEVIQ
                     YIQDSPLYKDTALLSEKGWANERIADPWLIAIAKKENYTIVTDEIKNVNLNDVNPSRN
                     AKVPDVCEKLNVQCISMNQFFKEISLSI"
     gene            657..833
                     /locus_tag="VVN16_002060"
     CDS             657..833
                     /locus_tag="VVN16_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573352.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
BASE COUNT          398 a          135 c          210 g          330 t
ORIGIN      
        1 ggcttaagtt gggcagtatt ttaaattcaa atgtggtagt tcctagaata gtggtttcag
       61 agaattatca aaatccatgg tttagggagt ggttagagga aaactacaaa aaagatttaa
      121 tagatcataa atcttatgct agagagtgga gtgaagtaat tcaatacatt caagatagtc
      181 ctttatataa agatactgct cttttatcgg aaaaaggctg ggcaaatgaa agaattgctg
      241 atccatggtt gattgctatt gctaaaaaag agaattatac aatcgtaact gatgaaatta
      301 aaaatgtcaa tcttaatgat gtgaacccta gcaggaatgc taaagttcca gatgtatgtg
      361 aaaaacttaa tgttcaatgt atttcaatga accaattttt caaagaaatt agcctatcta
      421 tctgatgtta atactagact aaaatcaaaa aacatcttac attagtagtt attaatctta
      481 caaacgaaga aatacagaaa attgaaaaac tcctaagaga ttcgtcgtag gccaaatatc
      541 acaaacgtct acagattata tttttgttca aaagagttat aaggaaatca tggatctgct
      601 agattgcaat aaaacaactg tttggaggaa tttgaagaaa tataaggagg tagcatatgt
      661 ccttagatat agataaagaa aagatgacaa tcatgggaat agcttttgaa aaccgatccg
      721 tttttaagag tgtttggtat gctttaagta caaatatgat tgaaggatgg cgacctacgg
      781 ttagtgatgt tgaaaaatta cgagatgaag ctcttgctct ggggatgact taatgaagcg
      841 caactgttat gagtcttatg actatgttga tccggataat ttatataccg atccgggttc
      901 ttcagttcta agaaataagc aggaaggaaa aaagcacgcg agcttgaata tcgtatgatt
      961 gctagtaaga gtttgaaatt atttatcaat cctatcttag ttaatagttt aaaagtagtt
     1021 tgtttcacaa ctgcataagc ttagaaaagt tgaaaagtga taactcttta ata
//
LOCUS       DAVHIT010000171         1036 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVHIT010000171 DAVHIT010000000
VERSION     DAVHIT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.173"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..616
                     /gene="pfbA"
                     /locus_tag="VVN16_002061"
     CDS             <1..616
                     /gene="pfbA"
                     /locus_tag="VVN16_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU5573353.1"
                     /translation="QPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFY
                     NLSGKYISFIKSNANEKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQ
                     SNSNNVIDSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKS
                     KEIVDVKQKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            600..>1036
                     /locus_tag="VVN16_002062"
     CDS             600..>1036
                     /locus_tag="VVN16_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU5573354.1"
                     /translation="MVKINKICSIQGSSAENEDTVGSQNQYFWIIDGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYYRLPTFAF
                     IFAKLSEKKLEYMMLGDCVMLVNEMEITDHRVDNLFEKGKNEI"
BASE COUNT          438 a          110 c          191 g          297 t
ORIGIN      
        1 acaaccaaat attgaattat tacgagttag tgataattta gtagtctcag agaatagtat
       61 attcggtggt aaagaaggaa ttgttattga ggattcaaag ggtaaaataa ccgttttaaa
      121 taaccaattt tataatttat ccggtaagta tatatcattc atcaaatcta atgcaaatga
      181 gaaagagcct gttatacgtg atagcgatgg taatttcaat attgtaacgg agaatgggct
      241 ttacaaaatt gtaacaaata acttaagtga taaaaacgaa aaagaaaaaa acaaagagga
      301 aaaacaatct aattcaaata atgtaattga tagtaaccag aagaacggag agtttaactc
      361 aagtaaagat aatagacaaa tgaatgacaa gatcgacaat aaacaagata ataagacaga
      421 agaagtaaac tataaaatag ttggagatgg cagagaaact gaaaatcata ttaataaatc
      481 taaagaaata gtagatgtaa aacaaaaatt accaaagact ggttcgaaca agattatgga
      541 actattctta acagtgacag gaattggttt acttttgaca ctaaaagggt tgaagtatta
      601 tggtaaagat aaataaaatt tgttcgatac aaggaagttc cgcagaaaat gaggatactg
      661 taggttcaca aaatcagtat ttttggatta ttgatggagc tacagattta tataattcta
      721 aagaggagat aggttattca gtctcagaag ttgtacatat tttatcagaa agtttgtcgg
      781 ttaattgtaa agaatcaaaa actcttaaac aaatatttga aactgcttta ctcgaggtta
      841 aagatgaaat cggtttaaac tcatatgaat taacagagta ttatcgctta ccaacatttg
      901 cttttatatt tgctaaactt tcagaaaaaa agttggagta tatgatgcta ggtgactgcg
      961 tcatgttagt aaacgaaatg gaaataactg atcatagagt agataactta tttgaaaaag
     1021 ggaaaaatga gataaa
//
LOCUS       DAVHIT010000172         1029 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVHIT010000172 DAVHIT010000000
VERSION     DAVHIT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.174"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            21..>1029
                     /gene="pcpA"
                     /locus_tag="VVN16_002063"
     CDS             21..>1029
                     /gene="pcpA"
                     /locus_tag="VVN16_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU5573355.1"
                     /translation="MKKTTILSLTTVAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AF"
BASE COUNT          396 a          147 c          171 g          315 t
ORIGIN      
        1 aaatatgtaa ggaaatttaa atgaaaaaaa ctacaatatt atcattaact acagttgcgg
       61 ttattttagc agcatatgtc cctaatgaac caatcctagc agatactcct agttcggaag
      121 taatcaaaga gactaaagtt ggaagtatta ttcaacaaaa taatatcaaa tataaggttc
      181 taactgtaga aggtaacata ggaactgttc aagtgggtaa tggagttact cctgtagagt
      241 ttgaagctgg tcaagatgga aaaccattca cgattcctac aaaaatcaca gtaggtgata
      301 aagtatttac cgttactgaa gtagctagtc aagcttttag ttattatcca gatgaaacag
      361 gtagaattgt ctactatcct agctctatta ctatcccatc aagcataaaa aaaatacaaa
      421 aaaaaggctt ccatggaagt aaagctaaaa ctattatttt tgacaaaggc agtcagctgg
      481 agaaaattga agatagagct tttgattttt ctgaattaga agagattgaa ttgcctgcat
      541 ctctagaata tattggaaca agtgcatttt cttttagtca aaaattgaaa aagctaacct
      601 tttcctcaag ttcaaaatta gaattaatat cacatgaggc ttttgctaat ttatcaaatt
      661 tagagaaact aacattacca aaatcggtta aaacattagg aagtaatcta tttagactca
      721 ctactagctt aaaacatgtt gatgttgaag aaggaaatga atcgtttgcc tcagttgatg
      781 gtgttttgtt ttcaaaagat aaaactcaat taatttatta tccaagtcaa aaaaatgacg
      841 aaagttataa aacgcctaag gagacaaaag aacttgcatc atattcgttt aataaaaatt
      901 cttacttgaa aaaactcgaa ttgaatgaag gtttagaaaa aatcggtact tttgcatttg
      961 cggatgcgat taaacttgaa gaaattagct taccaaatag tttagaaact attgaacgtt
     1021 tagcctttt
//
LOCUS       DAVHIT010000173          978 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVHIT010000173 DAVHIT010000000
VERSION     DAVHIT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.175"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            41..814
                     /locus_tag="VVN16_002064"
     CDS             41..814
                     /locus_tag="VVN16_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5573356.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRDNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTEGIFYCATFGENGVVNYLAN
                     LFKDEVNQDLENKTFTLQNGKRYLSRCFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            827..>978
                     /locus_tag="VVN16_002065"
     CDS             827..>978
                     /locus_tag="VVN16_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5573357.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFEL"
BASE COUNT          367 a           98 c          190 g          323 t
ORIGIN      
        1 tgtgatataa tgccaaatta tagagtggag gtcatttaaa atgattgata aagtaattga
       61 acaatataaa agtcatgata atcttgatat tcgagtagaa ttgcataaga agtattcaaa
      121 aaataaattg ggatttaata attggatatt ttctaactac caaatcactg atgaagtaaa
      181 ggttctagaa ttgggatgtg gtacaggaga actgtggaaa agtaatagtg attctataga
      241 taaaatgaag cagttgattg ttacagattt ttctaaagat atggtaaaat caacgaagtc
      301 agtgattgga aatagagaca atgtcaacta tgagataatg gatattcaaa agatttcttt
      361 tgagaatgaa acttttgata ttgttattgc taatatgctt ctacatcatg tgaatgatat
      421 tcctaaagct ctctctgagg tgaatagagt cttaaaaacc gaaggcattt tttactgtgc
      481 tacatttggt gaaaatggag ttgtaaatta tttggcaaat ttatttaaag atgaggttaa
      541 tcaagattta gaaaataaaa cgtttacttt acaaaatggt aaaaggtacc tgagtaggtg
      601 ttttaactct gtcgatacat tactctatga tgatgagtta caggtaacta gtatagatga
      661 cctagttaaa tacatccaat cgtttaaagg aatttcagaa ataggttcac tagaagagga
      721 aataatacgt aaaagattgg aaagtgagtt taataatggt atgttgatca ttcctaaaga
      781 atatggtatg tttatcgctc gaaaagaaag ttaagggaat gaaagtgtga aaaattacgg
      841 agaagctttt cgatatttta gaaaattaaa cggatattct ttagagtatg ctgcagctga
      901 ttctatatca aaatctcaac tttctagatt tgaaagggga gaaaatgaaa tttctctttc
      961 aacatttttt gaattatt
//
LOCUS       DAVHIT010000174          932 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVHIT010000174 DAVHIT010000000
VERSION     DAVHIT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.176"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(322..684)
                     /locus_tag="VVN16_002066"
     CDS             complement(322..684)
                     /locus_tag="VVN16_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5573358.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGGVGCNWWNKRDNIAAAAVSGG
                     MSVYQTYAIKKLIANRGMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGA
                     IAYGLDIVDGKFDGYLWA"
BASE COUNT          329 a          193 c          111 g          299 t
ORIGIN      
        1 tatttgataa agtaaagctt taaacccttc cccagacgtt agcttttatt caacccacta
       61 cagttgacaa ggagcccaaa ttattgataa aaactttagt catcatcgtc tctataaaaa
      121 tattttctat acactcttga taataccaaa attaatgcca atatcatact aagagctata
      181 ataatagaat ttattagatt tattttagta tatccaatca ctagtccaat aattggactg
      241 gtagacatgc ttagatataa taaaaataac aaaaaatgaa tttcaccaat tcctgcaaaa
      301 aaatgtttca tagctatagt tttacgccca taaataccca tcaaatttac cgtctacgat
      361 atctaaacca tatgcaatgg ctcctcctaa tgagaagact tcagcaacag tagccgcaat
      421 attaagagca ttagtaatag ttgccatact aactccacca atatatcttg ttatagcact
      481 tctaacgact cggctcatca taccacgatt agcaattaac tttttaatgg catatgtctg
      541 gtatactgac atccctccag aaactgcagc tgcagcaata ttatcacgtt tgttccacca
      601 gttacaacca acaccacccc catcaagata catcatctct tcttgctcaa gaacaacata
      661 attatttggt aatacgagtt ccatttattt tctccttttt tattaagcat agttaggata
      721 gtttcctaac aagtgatcgt tgactttgtt tatagcactt agttacctag ataagatatc
      781 ctttagcttg cttccaaaat ttaagtctgc acctataata tgagttcata ttagtactgc
      841 taaatcctat ctatagtaat gtcaaacacc taaataaagt catctctccg attcattcaa
      901 gaaatgaaga aaggcaaaaa atctttaaaa ta
//
LOCUS       DAVHIT010000175          923 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVHIT010000175 DAVHIT010000000
VERSION     DAVHIT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.177"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(276..446)
                     /locus_tag="VVN16_002067"
     CDS             complement(276..446)
                     /locus_tag="VVN16_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573359.1"
                     /translation="MKKENEYVILTTALLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGLLIVYKNNKN"
     gene            complement(459..904)
                     /locus_tag="VVN16_002068"
                     /pseudo
     CDS             complement(459..904)
                     /locus_tag="VVN16_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          301 a          179 c          121 g          322 t
ORIGIN      
        1 aaatcaaaga gcaaactagg aagcgagccg caggctgtac ttgagtacgg caaggcaaag
       61 ctgacgtggt ttgaagagat tttcaaagag tattaaacaa aaagaaaaac cgctactcct
      121 aagaatagcg atttaatcat gtttttaagc tactactgta gttgctctat tatttaagtg
      181 ttatgaataa cttcaatatc tttaaagctt tttgttagat ttataatttc aactatagcc
      241 atctctactc cttagtttac agcaaaaaat tatgcttaat tcttattgtt tttgtaaaca
      301 atcagcaaac ccaatactat tccattcaac aagcctaaag aaataccata ttcaactgga
      361 aaatctaaaa aaatagcaaa cactattcca atcatcaccc ctagtaaggc tgttgttaaa
      421 attacatatt cattttcttt cttcattttc tttacacctc atttcctttt tgtatataag
      481 ctgtagtact ctccaccaag agtcattaag tacttatgat ttcctgactc aacaatctta
      541 ccacctttca ttacaaaaat aatatccgca tccttaatcg ttgacaatct atgagctata
      601 attatttgag tacaaccctg actttgtata tactttgtta ttctttcctc attaatagtg
      661 tctaatgcac tagttgcttc atctaaaatt acaatactag gattatttat taatgcacgt
      721 gccagtgcta tccgttgcct ttgcccacct gaaatatttg accccatctc tgagatgata
      781 gtattaaatt tcatcggcat agccatgatt tcatcataga tttgaactgc tttacaaact
      841 tcctctatct tttgtgaagt aactagtgct ttaaagttat gttatcaaga atacttcggt
      901 tcaataaaaa tgaatcctgt gga
//
LOCUS       DAVHIT010000176          902 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVHIT010000176 DAVHIT010000000
VERSION     DAVHIT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.178"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(92..808)
                     /locus_tag="VVN16_002069"
     CDS             complement(92..808)
                     /locus_tag="VVN16_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5573360.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT          263 a          145 c          183 g          311 t
ORIGIN      
        1 atagaatctt ccaaaaatag gtaagcgctc cctttttggt gtggtataat aagcatagaa
       61 aaagcccaag cgattagctc aggctttctt cttagtgatc acggtcacat gagataaatt
      121 taatcttgta gtaatcagat cgtttgtaag tttcactgta ttctaaaact tggccagttg
      181 attcgagttt ggtgatttta gtttgtagga cagtagggaa ttgttcatcg actccgagga
      241 ctgaagccgc atgttctgga gttggaaaga ctatttcgtt gatttcttca aagtgttcat
      301 cattcatgtg aatgtggtag tctaacttga aacgattata gatagaacta tagtattcaa
      361 ggtttggata atttgcgttg atatattgtt ctgggatgta ggatgtatgg tagatataaa
      421 cgacaccgtt tgattcgcgg atacgttcaa tcttgtagta gaattgatcg ccgcgtagac
      481 ccaatttttc caagtaaaca agcttgtttc cgcgttcaat tgaaagaaca gttaccttat
      541 catctttagc attgaagagt tcaatatctg aaaactctac aagcttgtgt ttgcgcgcac
      601 gtgaaacgaa ggttcctttt ccttgttggc ggacaatata gccatctttg gcaaggtcgt
      661 ttaaggcgcg aacaactgtg atagagctga catcgtacat tgaaatgagt tctgcttcag
      721 tgtaaaattt atctccactg ctaaactgcc cagagatgat tttatttttt aattcgtctt
      781 ttatgtattg atatttggga attgccatat ttttacctct attttccttc tccatagaaa
      841 attttaccat aaaagcgaaa aaaatagtag tcttttgaat aaaaaattag aataatagtt
      901 tg
//
LOCUS       DAVHIT010000177          836 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVHIT010000177 DAVHIT010000000
VERSION     DAVHIT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.179"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            145..836
                     /locus_tag="VVN16_002070"
                     /pseudo
     CDS             145..836
                     /locus_tag="VVN16_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          291 a          151 c          176 g          218 t
ORIGIN      
        1 tttagaggct tatttaccat ggactaaagt tgtacaagaa aagtgcaaat aagaaatctc
       61 ctgattagga actatccgtg agttcactaa tctggagatt tttcaataga cttcctgcga
      121 aacaaaatat ggtataatag ttctatgaat gatgaagcaa gtaaacaact aactgatgca
      181 cgatttaagc gtcttgttgg tgttcaacgc acgacttttg aagagatgtt agctgtatta
      241 aaaacagctt atcaacttaa acacgcaaaa ggtggacgaa aacctaaatt aagcctagaa
      301 gaccttctta tggccactct tcaatatgtg cgagaatatc gaacttatga agaaattgcg
      361 gctgatcttg gtattcacga aagcaactta atccgtcgga gccaatgggt tgaagtaact
      421 cttgttcaaa gtggttttac gatttcaaga actcctctca gttctgagga cacggtaatg
      481 attgatgcga cggaagtaca aatcaatcgc cctaaaaaaa gaattagcga atgattctgg
      541 taaaaagaaa tgccacgcta tgaaggctca agcgattgtc acaagtcaag ggagaattgt
      601 ttctttggat atcactgtga actatagtca tgatatgaag ttgttcaaaa tgagttgcag
      661 aaatatcgga caagctggta aaatcttggc cgacagtggt tatcaagggc tcatgaagat
      721 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagcaaactc aagccgctaa
      781 cagctgaaga taaagcctgt aaccatgcgc tatctaagga gagaagcaag gttgag
//
LOCUS       DAVHIT010000178          835 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVHIT010000178 DAVHIT010000000
VERSION     DAVHIT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.180"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..778)
                     /locus_tag="VVN16_002071"
     CDS             complement(<1..778)
                     /locus_tag="VVN16_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573361.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLK"
BASE COUNT          298 a          151 c          116 g          270 t
ORIGIN      
        1 attttaaaaa aataggcttc gtaccaataa tagaggataa ttgacctcct aattcaccta
       61 gcgctgtaaa aataacacct gttagtgctc ctattgcttc aatagtacca attttcacta
      121 ttccttttat tgcaagatag cctgttaaaa aaacgagaga tatctgaaaa aaaatattga
      181 gaaagaagct aatagcgcct gctaacgttt ctacagttgt ctttctttgt ataaccatct
      241 ttaataaaat tcctgcttct ttaattttct taggcaatac atataaaaga ttcaaggacg
      301 ctaacacatc aaatccattc aatatagtct cactagattt taaaaaagct tcattttggc
      361 tagttaaatt tagactaact tctcgcattt tcgatgcaaa gatttttggt acaagtagca
      421 taatcattaa tgaaaacaag gtggctacag tcaatgacca atgatagtga ttaagagtca
      481 caactgcaaa tatagtacca gaaattcctt ttattactaa aaaaagttgt ttaaaagcct
      541 gatcatttaa agtctgaaca tcattattta gccacgaaag atatgttcct gatgatttac
      601 tatgaaattc ttgataggta gagttagaga tgtctgtggc aactctattt cgaatctcta
      661 gattaaactc ttggatcact tcaacctgat aatttttcac tacccagtca aggaatatta
      721 tcccacacca gacaatcatt tggtagattg acaatttcaa aaaccgctct aaattcatcg
      781 caattaattc attcaacacc agagcattaa tagttgctgc ataaattagc aataa
//
LOCUS       DAVHIT010000179          798 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVHIT010000179 DAVHIT010000000
VERSION     DAVHIT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.181"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..417
                     /locus_tag="VVN16_002072"
     CDS             <1..417
                     /locus_tag="VVN16_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5573362.1"
                     /translation="LVKNVSKDKIFQKVKEREKRDKEIIELAKSKNFPISPEEFLTTI
                     TESLNEYNPKDNYEVLNALALKTSNILFWLDIVFQKTSSEFYIVFEDKEFNQEYYNKL
                     YKKFENLFEEIKSLALQEAKKDKENLDDFALLWGID"
     gene            578..>798
                     /locus_tag="VVN16_002073"
     CDS             578..>798
                     /locus_tag="VVN16_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5573363.1"
                     /translation="MRKYDIPKLLLSGENQGVEFKEAKNSFPKDGMKTICSFANTNNG
                     LLILGVSENTKNKDFYISGVNDPDKVLDE"
BASE COUNT          315 a          120 c          137 g          226 t
ORIGIN      
        1 ctcgtaaaaa atgtttcaaa agataaaatt tttcagaaag taaaagagag agagaaaaga
       61 gacaaggaaa taattgaact agcaaagagt aaaaatttcc caatcagtcc tgaagagttt
      121 ctcactacta tcactgaatc attaaatgaa tacaatccca aagacaacta tgaagtttta
      181 aatgcccttg cgctaaagac aagtaatatc ctattctggc tagatattgt atttcaaaaa
      241 acaagtagtg aattctacat agtttttgaa gataaggaat tcaaccaaga atattacaac
      301 aaattatata aaaagtttga aaatctattt gaagaaatta agtctttagc tcttcaagaa
      361 gctaagaaag ataaagaaaa tctagatgat tttgcgttac tttggggtat agactaaccc
      421 acgcgccaca atgttcaaca atcctgaagc gcgtgtgcga atgcgtgtgc gaataatcta
      481 ctttcaaaaa aacctttaat agcactgtta gctaactaaa aataaactat ttttcccgcg
      541 cgtgtgcgaa tgcgtgtgca aattgattgg agaaaaaatg agaaaatacg atataccaaa
      601 attgttatta tcaggggaga accaaggtgt tgaattcaaa gaagctaaaa attctttccc
      661 taaagacggt atgaaaacaa tttgttcctt cgctaataca aataatggtc tgttaattct
      721 aggtgtctca gagaatacaa agaataaaga tttttatatt tctggagtta atgatcccga
      781 taaagtactg gacgaatt
//
LOCUS       DAVHIT010000180          786 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVHIT010000180 DAVHIT010000000
VERSION     DAVHIT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.182"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(35..403)
                     /locus_tag="VVN16_002074"
     CDS             complement(35..403)
                     /locus_tag="VVN16_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU5573364.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGCNWWNSRGSFATVLDVDL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALTVTGFSLG
                     GAIAYGVDWADGSLDGYIFA"
BASE COUNT          264 a          178 c           91 g          253 t
ORIGIN      
        1 tatttcacca attcctgcaa aaaaatgttt cattttaagc aaaaatataa ccatctaagc
       61 taccgtcagc ccaatcaacc ccatatgcga ttgctccacc tagtgaaaat ccagtaacag
      121 ttagtgcagt attgactaaa tggccagctg cactacctac atgtttaatt attaaactac
      181 gtaatgttct cgtaatagcc cctctattag ctgagatagc tttttttatc gcataagcag
      241 aataaattgt tgcaccacca ctatagatgg ccaaatctac atccagaact gttgcaaaac
      301 tacctctact attccaccag ttacaaccaa caccaccccc atcaaggtac atcatctctt
      361 cttgctcaag agcaacataa ttatttggta atacgagttc catttatttt ctcctttttt
      421 tattaagcat ggttaggatg gtttcctaac aagtgatcgc aggctttgtt tagttaccta
      481 aattagactt ccttccttta tcttatttta aatatttaaa accagttact gcttgaagct
      541 taacagttta cttaattcaa ataacagcta atcactgatt ccctgatttt atcaaatata
      601 ctaataacaa caattgtgat acgatcatca ttataagaac aacatttaac gaatcaaagt
      661 ttttccgaga taataataaa gcaacagcaa gcagtattat tactaaagat tgaataacta
      721 ctattactct taacatttta tcctccacta acaactcaag ctcccttaca ttatatcgac
      781 cagccc
//
LOCUS       DAVHIT010000181          770 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVHIT010000181 DAVHIT010000000
VERSION     DAVHIT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.183"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            30..>770
                     /locus_tag="VVN16_002075"
     CDS             30..>770
                     /locus_tag="VVN16_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573365.1"
                     /translation="MLPIWGLWIELHLLVSNLQLNLEIPFDFVVSTSLTFFVLILSKI
                     VLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSPDDLRSRIIWS
                     FILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWQKEDSFES
                     DLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCT
                     GLVELLLYEMSISNNNNWQEKVDKIRIQL"
BASE COUNT          247 a          108 c          130 g          285 t
ORIGIN      
        1 tgatacgtaa actaatcatt tttttattaa tgctccctat ttggggcttg tggatagagt
       61 tgcatttgtt agtgagtaac ttgcaattga atctagaaat tccctttgat tttgtggtta
      121 gtacgagtct tacttttttt gttttgatat tatcaaaaat agttttagac atactatacg
      181 cattgaaaga tttgtacaag aaagaagctc ttattacgat ttttcccttt atttttatag
      241 gaaggaagaa agtaaatgtg aggttctcgc cctatttttc attccatcgt aaaagtttgt
      301 cgcctgatga tttaagatct aggattatat ggagttttat tcttgaaatt gctattatcc
      361 tagtatttat tttaaaaatt ccttttgcaa tcatcatgct tactacaata tttttctgga
      421 ctataatgga tatcaatcat ctcgttttta ataagacgga atttcttttt aatcaaaaca
      481 aatggcaaaa agaagattcg tttgaaagtg atttgacgaa aacgttaaag gataagatac
      541 aaaaatcaga gctaagttat tctgacttaa tgtctcttca gctgtatgat gcgatgaacc
      601 aatccacctt tttaacggat agtgaacttt ttgaggatat tttgaaaaaa atcgaagatt
      661 ctcataacac ccttctgtgt acaggtcttg tcgagttact gctgtatgaa atgtctatta
      721 gtaacaataa caactggcaa gaaaaggttg ataaaattag aatacagctc
//
LOCUS       DAVHIT010000182          725 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVHIT010000182 DAVHIT010000000
VERSION     DAVHIT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.184"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..>725
                     /locus_tag="VVN16_002076"
     CDS             <1..>725
                     /locus_tag="VVN16_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5573366.1"
                     /translation="LQRRYFEKTKRKLTVPKSYQEFDELSEFFTLTENPFSPTLYGHS
                     LALSSSVRAACEFIPRFRERLAQSRMNLAVLPQVLTEYEKSYQFTEKSFNSSWNDFVT
                     NLNSGKTSMEIIFSNYTSPLFDGLNVSAQFEFATATIPGNTPVIGGGSIGISKYSNRV
                     EECLNFINWLYSEEISILLTSLGGFLPSKYVMQNRMLQFQYPWLSSLEDTFSIGSRTK
                     WYGFNTDFRFEQMLGQELIESVK"
BASE COUNT          240 a          101 c          133 g          251 t
ORIGIN      
        1 ttacaaagac gatactttga aaaaacaaaa agaaaattaa ctgtaccaaa aagttatcaa
       61 gagtttgatg agttaagtga attttttaca ttaacagaaa atccattttc acctacactt
      121 tatggccata gtttagcttt aagttcttca gtgagagcag catgtgaatt tattcctcgg
      181 tttcgtgaga gattagcaca atctagaatg aatcttgctg tacttcctca ggttttaacc
      241 gagtatgaga aaagttatca gtttaccgaa aaatcattta attcttcgtg gaacgatttc
      301 gttacgaact taaacagtgg taaaacttca atggaaataa ttttttcaaa ttatactagt
      361 ccattatttg atggtctcaa tgtttctgct cagtttgagt ttgcaactgc cactatacct
      421 ggtaatactc ctgtgatagg tggtggctca attggaattt ctaaatattc aaatagagtg
      481 gaagaatgtc taaactttat taattggtta tattctgaag agatttctat tttactaaca
      541 tctctgggtg gttttttacc atcaaagtat gtcatgcaaa ataggatgtt acaatttcaa
      601 tatccatggt tatctagttt agaagataca tttagtattg ggagtagaac aaaatggtac
      661 ggctttaata ctgattttag atttgagcag atgttagggc aagagttaat agaaagtgtg
      721 aagca
//
LOCUS       DAVHIT010000183          692 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVHIT010000183 DAVHIT010000000
VERSION     DAVHIT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.185"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..479
                     /gene="rlmH"
                     /locus_tag="VVN16_002077"
     CDS             <1..479
                     /gene="rlmH"
                     /locus_tag="VVN16_002077"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU5573367.1"
                     /translation="KIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPD
                     KASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFSTL
                     TFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            523..596
                     /locus_tag="VVN16_002078"
     tRNA            523..596
                     /locus_tag="VVN16_002078"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Arg,seq:tct)
BASE COUNT          236 a          100 c          138 g          218 t
ORIGIN      
        1 tgaaaattaa agttgtaaca gttgggaaac tgaaagaaaa gtatttaaaa gatggtatcg
       61 cagagtattc aaaacgaatt tctagatttg ctaagtttga aatgattgag ttatcagatg
      121 aaaaaacacc agataaggcc agtgaatcag aaaatcaaaa gattttagaa atagaaggtc
      181 agagaatttt atcaaaaatt gctgaccgtg atttcgttat tgtgttagcc attgaaggga
      241 aaactttctt ctcagaagaa tttagtaagc agttagaaga aacttctata aaaggatttt
      301 ctactcttac ttttattatt gggggaagtt taggattgtc atcatctgta aaaaatagag
      361 ccaatctttc tgtcagtttt ggtcgcctaa ccttgcctca tcagttaatg agactagttc
      421 ttgttgaaca aatctatcgc gcttttacga ttcagcaggg attcccctac cataaataga
      481 aaattgactt ttaattgaat ttttggtaga ataattgtgt taggtctcat agctcagctg
      541 gatagagcat tcgccttcta agcgaacggt cgcaggttcg aatcctgctg ggatcaatat
      601 aatagcaaag ctgggaattt tcccggcttt tttcttaaaa aagtacactt tgggagaaaa
      661 aaatgacagt tgagagaatt ttatctaaaa cg
//
LOCUS       DAVHIT010000184          671 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVHIT010000184 DAVHIT010000000
VERSION     DAVHIT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.186"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..200)
                     /locus_tag="VVN16_002079"
     CDS             complement(<1..200)
                     /locus_tag="VVN16_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573368.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSS"
BASE COUNT          237 a          126 c           93 g          215 t
ORIGIN      
        1 gaggaaacta gtaatagcaa agtatgtaag cattgagaca aaatatagaa taacggtatc
       61 gccaatacaa aggttaagat ggcatgagag ggggttccta agatgtccaa ttttataaat
      121 actgggctga cacaaaatat cgtgcttata agcagtaaaa ttattaaaat tgtgtcaaaa
      181 aaataacgtt tatttttcat ttagtctact ttccattgtt aaataatact actgctaaca
      241 ccaagccaac ttagcacatt gcatcaccta atcctgcgct acttgtccat aatcctttaa
      301 tgtttctact aaaattattc caatctcccc catcaagata gcttatttat cctagacaat
      361 gccctactgc aacagtataa ataagattca tcagccatct tttctggcaa tcatatgagt
      421 agcctaatga tatttctagt tactcaaaaa tatctagcta tactagagtt atttctaatc
      481 cctaggacac ttgttaatcc aaatgtaaca aattttcacc ccttaatagg actctttctg
      541 gaaaaaatat aaggtctatc tttataaaaa aatctatcat attgggatgg cacgcatctg
      601 gaatttaaac aagctataaa ttcatttata actactattt gtttagaggc aacagcatct
      661 tgtataccgt c
//
LOCUS       DAVHIT010000185          669 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVHIT010000185 DAVHIT010000000
VERSION     DAVHIT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.187"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(114..>669)
                     /locus_tag="VVN16_002080"
     CDS             complement(114..>669)
                     /locus_tag="VVN16_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU5573369.1"
                     /translation="VMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEF
                     SVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSN
                     QGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWFTVSGKWYYTYN
                     SGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT          214 a          119 c           93 g          243 t
ORIGIN      
        1 aaattggttt aaatagcgta gtcttttaaa ctagttttga gaatccaaaa aatcttccta
       61 catatgtaag aagatttttt agttcagaat gattagattt agctaatgga tacctatcct
      121 acccactcac cgttagcatt gactcgatag ccatcgggtg tagtcgtgtt tactaataaa
      181 tctcctgaat tataggtata gtaccattta ccagaaactg taaaccaacc agtagccatt
      241 gaacctgatt catttaagta ataccataag cctttgtctt taacccaacc agtagccatt
      301 gaacctgatt cgtttaagta ataccataag cctttgtctt taacccaacc aactactccc
      361 tgattagaat tctttttagg tttagctaca tcagtttctt gttttatatt atctactttt
      421 tcaacaatga tatcattact agtagataaa tttgatttaa gaacatcttt tatatgttct
      481 gatgttacat agaacttaac agtttcagga attgcaaatg tatctttttt cacagaaaac
      541 tctgtacttt tattcttaat atgaatttcc tttaatgaat ctaagacgcc acttaggaag
      601 aaggacggca atgagttgat attattacca attgttaaac tttttaattt tggtaaaccg
      661 ttcataacg
//
LOCUS       DAVHIT010000186          664 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVHIT010000186 DAVHIT010000000
VERSION     DAVHIT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.188"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            1..645
                     /locus_tag="VVN16_002081"
                     /pseudo
     CDS             1..645
                     /locus_tag="VVN16_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          242 a           57 c          144 g          221 t
ORIGIN      
        1 ttatttgtgt atctgaaact atgaaaaaaa gattggagag tataataaaa aaataagaag
       61 atagttgcaa taaataacgg aattaatgta tcaaatagcg atttagatgt agtaggtgtt
      121 caagacttta aaaaagaatt ttgtattcca aataacaaaa aaatcatttg ttatgttgaa
      181 aggttggatc cagaaaaaag gccggataga ttccttgaat ttgcagaaaa attattttta
      241 gtcagagaag atgtgatttt tattatggct ggaaatggta gtatgtgggc tgctttgaaa
      301 gaaaaaattt gccatttgaa atgtagagat aatttcaggt tacttggtga aatctatcca
      361 gctactatag tttaccaaat atcggattta ttgtatattc cttctgatac agaaggaata
      421 cctatgtgtg ttttagagtc aatgtcacaa ggaactccgg ttttagctag taatgttggc
      481 gggttaagtg aaattattga acatagggtt gatggatttt tatttgagaa ggaagatgtt
      541 gagggagtgt gtgcttgtgc taatttttta ctcaatgatt ctgagtattt gaaatatata
      601 ggtgagaata gtaaatcaaa aataagaaaa catgagagta tatgatgaat tattagagaa
      661 gagt
//
LOCUS       DAVHIT010000187          633 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVHIT010000187 DAVHIT010000000
VERSION     DAVHIT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.189"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..617)
                     /locus_tag="VVN16_002082"
     CDS             complement(<1..617)
                     /locus_tag="VVN16_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5573370.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYTEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNR"
BASE COUNT          215 a          108 c           78 g          232 t
ORIGIN      
        1 tttctattta agtatgtgtc aattactata cttatccatt catttgcctt tgctttaaag
       61 aaaaaatcag cggcaggagc gtccatctta caatttaata tttccattgc cactctttct
      121 aaggcctttg taagtattat ttgattcggt tgaagcaagg ttgaataaaa agattctgga
      181 ttaatgttaa tagatgctaa atgtttttct attagctctt ttttaaaacc aatggaaaca
      241 gcaagataac aacaattctc gtgtaataaa aaaacaaaat catcttttat attatcaaaa
      301 tcaaaagtac ataaagagtt tgcggtaata gtttgatacg gattaaactt ttctccgttt
      361 gcactgacaa tgtaacttga ataaattgaa acatagtctg acatactata agtgctattt
      421 tgaactactt cctctttgat ataaaaatca tgtatatcga taatgaagct gcctccttca
      481 taaaaccaat ataggccttc tgtataagtt gaattctctt ttttccaaca aaatgtatga
      541 cctgcacttg aatacttctc attgttttta cattcatcta ctttcatata atcttttaca
      601 aaatcataat atgacataac acactatccc ttt
//
LOCUS       DAVHIT010000188          632 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVHIT010000188 DAVHIT010000000
VERSION     DAVHIT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.190"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
BASE COUNT          196 a           73 c          109 g          254 t
ORIGIN      
        1 caaaaaaata tgctattatg ttaaaaaaat atttttcaaa atataaatgg acggatttat
       61 tttggatttt atttgttatt ttgacctgcc tctatattgg taaccatgat ttgtttactc
      121 tcaatcatca agaattctct tttcgtggta gcgtttgggg tctggtactg gccttatatc
      181 acttattgtt cattgataag tttgtaattt cgaatcgaaa ataaagatta gaactatgat
      241 tgactggcta cttttaatat tgattttgga ggaagatttt gtctctatta tttattattt
      301 taaatttatt tattttgtat aagatctatt ctttgaggcg ggaaaaatcg aaatacttga
      361 tttatacggt ctatatcata tttggggtaa atgtagtata tggtattcaa tggttattaa
      421 aagaactgat ttcaaatatt tccacttaat atgtatggaa actataacaa aacgcataag
      481 attaaggttt gtgtacctgt tcttatgcgt ttttgtaata ttgaagattt tcggtaagtt
      541 ttctactttt gcgaagtcta gtttatactc aatgaaaatc aaagagtaaa ctagccgtag
      601 gttgctcaaa gtacagcttt gaggttgcag at
//
LOCUS       DAVHIT010000189          628 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVHIT010000189 DAVHIT010000000
VERSION     DAVHIT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.191"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            46..>628
                     /locus_tag="VVN16_002083"
     CDS             46..>628
                     /locus_tag="VVN16_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEU5573371.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTFVRAEESPQAVEKSSSE
                     KKYDEAKAKSDAAKKNYDDAKKKAAEAQKKYEDDQKRTEAKAEKERKASEKIAEATKE
                     VQQAYLAYLQASNESQRKEADKKIKEATQRKDEAEAAFATIRTTIVVPEPSELAETKK
                     KAEEAKAEEKVAKRKYDYPQLFTPNLKTIQNPCL"
BASE COUNT          266 a          104 c          133 g          125 t
ORIGIN      
        1 ataagtatag tctaaaaatg actatcagaa aagaggtaaa tttagatgaa taagaaaaaa
       61 atgattttaa caagtctagc cagcgtcgct atcttagggg ctggttttgt tgcgtctcag
      121 cctacttttg taagagcaga agaatctcca caagctgttg agaaatcttc atcagagaag
      181 aaatatgatg aagcaaaagc aaaatctgac gctgctaaga agaattacga cgacgctaaa
      241 aagaaagcag cagaagctca gaaaaagtat gaagatgatc agaagagaac tgaggcaaaa
      301 gcggaaaaag aaagaaaagc ttctgaaaag atagctgagg caacaaaaga agttcaacaa
      361 gcgtacctag cttatctaca agctagcaac gaaagtcaga gaaaagaggc agataagaag
      421 ataaaagaag ctacgcaacg caaagatgag gcggaagctg catttgctac tattcgaaca
      481 acaattgtag ttcctgaacc aagtgagtta gctgagacta agaaaaaagc agaagaagct
      541 aaagcagaag aaaaagtagc taagagaaaa tatgattatc cccaattatt cacccctaat
      601 ctaaaaacca tccagaatcc ttgcctta
//
LOCUS       DAVHIT010000190          595 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVHIT010000190 DAVHIT010000000
VERSION     DAVHIT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.192"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            365..526
                     /locus_tag="VVN16_002084"
     CDS             365..526
                     /locus_tag="VVN16_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573372.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT          239 a           81 c          113 g          162 t
ORIGIN      
        1 taaaaagatt aataactctc gataaatttt gaaccttaac aattgaatag accaagacaa
       61 ggcgttaatt atttttggag cgtaataacc tatccgtcaa gatctttcgg ctgaataatt
      121 cggcaaaaaa agttctgcta agcaaaaaga aagtgagcga taagtaagag tttgaatata
      181 gggagggata ccctatccgc tatgaagaaa atgaggataa tgatacttct aaggttgaag
      241 ccggctcatc cggaatagag gcggaggtga gattcctatg ttgctaatcc aaagcacttg
      301 tcaatgtcaa aaaacttaat taaacaaata attattagat ataaaacttt agaggaggta
      361 tatattggac aatgactgga atggattggc tgatttaata gcaaatctga ttgcaaaata
      421 tgctggtgct ttagatttag ataatctacc cgatccaacg ccagcaaaaa atcaagagat
      481 gaaaaatagt tttgacatgg caaaaacaca aattgagacg gactaataaa agtgttataa
      541 tatacttgaa atgatagtcc aaacttaaat taaaaaaatt atatatgata tagaa
//
LOCUS       DAVHIT010000191          584 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVHIT010000191 DAVHIT010000000
VERSION     DAVHIT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.193"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            22..300
                     /locus_tag="VVN16_002085"
     CDS             22..300
                     /locus_tag="VVN16_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEU5573373.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIESLVDDILQFSLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWDLLEFQKFRAFLA"
BASE COUNT          224 a           67 c          109 g          184 t
ORIGIN      
        1 aatagtggag aggaagttag aatgaaaaaa ttcgacaatt atattattga gaagccttgc
       61 gattctaatt cagataaact gcaaaaaatc ttaataattg aaagtttggt agatgatatt
      121 ttgcaatttt ctctcagaat caataatagt gtaggagaga ttttcctcct acaaccgttt
      181 caaaagaaaa ctatctttat tccatgttat tttgaggaag atattgtgaa agtcaaagat
      241 gatgataaag ttgagtggga tttgttagaa tttcaaaaat ttagagcatt tttggcttag
      301 taatatgtgt tgaaggctca aaacctatgg taaaaaagta gctttgaaaa cgtattacct
      361 ccaaagattt agttaaataa tgatttaaca caaaaagaaa ttattgaagt tctggaaaga
      421 tgttgtttca gtattgagaa aaggtgggaa aaacttgcga ttttcacaga gaaaggaagg
      481 aagaaaaagt atagaaatat agtcaattga aacaagaaca gaacaaaaga accttttgtg
      541 ccatattttc tcctttcgct ttacaattgg cttgaacacc ttta
//
LOCUS       DAVHIT010000192          578 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVHIT010000192 DAVHIT010000000
VERSION     DAVHIT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.194"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(159..512)
                     /locus_tag="VVN16_002086"
     CDS             complement(159..512)
                     /locus_tag="VVN16_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5573374.1"
                     /translation="MELVLPNNYVVIDEEEMMYFDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYYIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
BASE COUNT          194 a          127 c           73 g          184 t
ORIGIN      
        1 atttttcaca gaatatagag gtagtgatac tattgagtag ataaaagcta aaaaaataag
       61 caaaaaatac tccgtatcat ttataatcgc cctgaatgat aaaaatatat acacccaaca
      121 cacgatgatg tgtatttttc gaatcttatc tacattactc atcttactgt tgcagatatg
      181 aatccatccc aaggataagg gttcccgtta atatcacaac ctctattaag aacaccatat
      241 ccaatatagt aagctatttt ccctgctgct gtagccaata cacctgctgc tgcaccaata
      301 agccagcctc caagacctcc aaccttaata tagtttatta gttttttggt taaaactgca
      361 gctccagcta atgctataaa agttcctgaa ctcatagcta aagctgcgca aattccttga
      421 caagcacgct tgcttaaata agctccccca tcaaaataca tcatttcttc ttcatcaatc
      481 acaacataat tatttggtaa tacaagttcc atttgttttc tcctccttag ttgaaattgt
      541 ttcatttgtt ttacaaatag gacgaactta aactatct
//
LOCUS       DAVHIT010000193          575 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVHIT010000193 DAVHIT010000000
VERSION     DAVHIT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.195"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..248
                     /locus_tag="VVN16_002087"
     CDS             <1..248
                     /locus_tag="VVN16_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU5573375.1"
                     /translation="VLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFGENK
                     YRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            323..520
                     /locus_tag="VVN16_002088"
     CDS             323..520
                     /locus_tag="VVN16_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5573376.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          201 a          103 c           77 g          194 t
ORIGIN      
        1 tcgttttaag aatcccagtt attcctaatt ttaacaatag tttagaggat gcagaaaaat
       61 tcgctactct atttaactca ttaaatatcg accaagttca actactccct tttcatcaat
      121 ttggtgaaaa caaatatcgt ttattaaatc ggaaatatga aatggatgga atcaacgcac
      181 ttcatcctga agatcttatt gattatcaaa aggtatttct gaaccaccat attaattgtt
      241 atttctagtt tatttccttg aaatgctcta gctatttgca gataacaagc atctatataa
      301 ggttatcaaa gaagacttcc ccatgactct aaatgaatat atcttacaat atcgcctgaa
      361 acaagctata gataagatgg ctgaatctcc caactcccct ttaagcgcta tctctgacca
      421 agttggattt tcagactata aatattttgc caaagtattt aaaaagtatc tccatatttc
      481 cccaaagaaa ttgaaatcac tcggaagaat agtcaaatag gctattcttc tttttgtaga
      541 gaattttact ctaatatagt gaattgaaat aagat
//
LOCUS       DAVHIT010000194          569 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVHIT010000194 DAVHIT010000000
VERSION     DAVHIT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.196"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..>569)
                     /locus_tag="VVN16_002089"
     CDS             complement(<1..>569)
                     /locus_tag="VVN16_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5573377.1"
                     /translation="LSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGYPLSQQLQPS
                     AKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNGNFLYRHIYS
                     ELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALEFMRYKVIYY
                     EDFPYVSDSSMVSYMGKTKELKMYQEELD"
BASE COUNT          192 a          112 c           74 g          191 t
ORIGIN      
        1 catcgagttc ctcttgatac atttttaatt ctttagtttt tcccatataa ctaaccatac
       61 tactatcact tacatatggg aaatcctcat aatatattac tttataacgc ataaattcaa
      121 gcgcccttcc aatactattc acaaaaacat gatcaacatg gtcaccaagt gaaagcggac
      181 aatatacgac acatttgtcg tctaaatgca ttaacagctc ttttatgata tcattcttta
      241 atgtgtcctc attttttaat tcactataga tatgacggta tagaaaattg ccatttctat
      301 ctttcctata gagacattca tagtacgata agtgtctaaa atcacattgt agacgttcac
      361 aagctaacct atcttctttc ttcctttctt caatcggata tttcccaagg ttacacaact
      421 tatgaaattg cttagcagag ggctgtagct gttgactcaa agggtaacca gaaaatatag
      481 taataacaag tacaatttct ccttctgaag ttaattttga aatataatca ccacaggaaa
      541 aaattgcgtc atctaaatgt ggagataaa
//
LOCUS       DAVHIT010000195          560 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVHIT010000195 DAVHIT010000000
VERSION     DAVHIT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.197"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..482)
                     /locus_tag="VVN16_002090"
     CDS             complement(<1..482)
                     /locus_tag="VVN16_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5573378.1"
                     /translation="MGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKNL
                     ALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRD
                     IFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNR
                     S"
BASE COUNT          189 a          100 c           66 g          205 t
ORIGIN      
        1 gaacgattta ggtaaatatc tttaagcgtc tcttttttag tttcaaaatc aactacttta
       61 atagagtttt cgttaaaaaa tttaaaaagc tcactacttt gaatattccc agacatagta
      121 attctcaatc cctcttcctg aacaatatct ccaaattctt gtttggtaat gaaaatgtct
      181 ctatctgtag ctgatgaaaa agctatctca aacaagaaat tatgactatc ttttcctact
      241 ttttgaaatg tcaaaagccc gttctccaag aaaagaactc tctcacaaat ttcttcaatg
      301 tcttctaatt tatgactcga tattaaaatt cccacatttt catgtaaagc taaatttttt
      361 agaaccgcta aaactatttg tgatgattca atatctaaac cattagtcgg ttcatctaaa
      421 atcaatatat caggttccgt aacgagagtt agaagcaaag ctaatttttg ttttgtaccc
      481 aaagaatagg tctttacttt tttattaata gactgagtca aatctaactc ttggatcaaa
      541 cttctaaata tttcttgatt
//
LOCUS       DAVHIT010000196          556 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVHIT010000196 DAVHIT010000000
VERSION     DAVHIT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.198"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            138..437
                     /locus_tag="VVN16_002091"
     CDS             138..437
                     /locus_tag="VVN16_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5573379.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          200 a          101 c          106 g          149 t
ORIGIN      
        1 tgtcttaaaa aaatggtata ataaaatcaa tacttgggct tgatggctat gctactaata
       61 acaattagga gagaaaatca ggcacttgtt aacaacaagg attatcccct tgagatgaaa
      121 ggaactttag aaatcttatg atgaacatgc aaaacatgat gcgtcaagca caaaaacttc
      181 aaaaacaaat ggaacaaagc caagctgaac ttgctgctat gcaatttgtt ggcaaatctg
      241 ctcaagatct tgtccaagcg accttaactg gcgataagaa agttgtcagc attgatttca
      301 atccagctgt cgttgaccca gaggaccttg agactctttc tgatatgacc gttcaagcca
      361 tcaactctgc tcttgaacaa atcgatgaaa ctaccaagaa aaaactgggt gctttcgctg
      421 ggaaattacc tttctaaaaa caaggagcta gaacaatgct tgtcgataac aaaggctaag
      481 aaaggtgcaa aaatgactct ataatatttg tagtgggtaa atccactata gatattatgg
      541 agcctatttt attgta
//
LOCUS       DAVHIT010000197          524 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVHIT010000197 DAVHIT010000000
VERSION     DAVHIT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.199"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..492)
                     /locus_tag="VVN16_002092"
     CDS             complement(<1..492)
                     /locus_tag="VVN16_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573380.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQSYLGGNTV
                     WIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAIFENLLDSY
                     LIQN"
BASE COUNT          188 a           81 c           81 g          174 t
ORIGIN      
        1 attctgaatc aagtaggagt caagcaaatt ttcaaaaata gctaacaata tgattggtgt
       61 aaggtttttg tattcacatg tcaagtattc cgagtatcgt gcagatgata tttcttttat
      121 ataacttttt gaaagcaaga ttttttcaaa ttcatcatct attattaatc ttctatttcc
      181 aatccaaacg gtattaccac ccaaataaga ttgtgcaaat agcaatatgt aagaaaatat
      241 gaacgtaaat actgaaagag gtagaattct atgcgtaaaa aaatagacga taatagtagc
      301 caggagtaaa gatatctctc gtgacaatcg aattcttttc agcatctttt ttaacagtcc
      361 gtaaggttgt ccttggcaat attcttgaac taaattaatc ggaatgacat ctctcaccat
      421 ttctggaaag tagagtaagt ttataggctg aaatctccag ctcccatcgt aagtaaatgg
      481 atatagaacc aaatacttaa aagatttttt tcttataaac ttaa
//
LOCUS       DAVHIT010000198          518 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVHIT010000198 DAVHIT010000000
VERSION     DAVHIT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.200"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..404
                     /locus_tag="VVN16_002093"
     CDS             <1..404
                     /locus_tag="VVN16_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU5573381.1"
                     /translation="DYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFSNKL
                     KRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTANI
                     KNVMLVSPTEAEAIKLFSNTFLALRVAFLMN"
     gene            368..>518
                     /locus_tag="VVN16_002094"
     CDS             368..>518
                     /locus_tag="VVN16_002094"
                     /inference="COORDINATES: protein motif:HMM:NF013175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573382.1"
                     /translation="MLSSPCCFFNELDSFAERRSLNAEVVIKGVCLDPRIGNFYNNPS
                     FGFGGY"
BASE COUNT          172 a           64 c           99 g          183 t
ORIGIN      
        1 cagattatga tgagaaaatt ggtagtttta atacatatga aatcgaacaa acggtatcga
       61 agattctgag ggtaaaacct aatggaaaga ttattttaaa gtcaacagtt ccgatcggct
      121 tttcaaacaa attaaaaagg ctgtttgata caaaaaatat catttttgtc cctgaatttt
      181 tgagagaagg ttgttctata tatgataatt tatatccaag tcgcatagtt gttggagatg
      241 agacagttga aggaagaaaa attgcagagt tgttcctttc gattagtact catagtactg
      301 ccaatattaa aaatgttatg ttagtttctc ctactgaagc agaagcaatt aagctttttt
      361 ctaacacatt cttagctctc cgtgttgctt ttttaatgaa ctagattctt ttgctgagag
      421 gagaagctta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa gaattggaaa
      481 tttttataat aatccttctt ttggatttgg aggatatt
//
LOCUS       DAVHIT010000199          498 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVHIT010000199 DAVHIT010000000
VERSION     DAVHIT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.201"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..>498
                     /locus_tag="VVN16_002095"
     CDS             <1..>498
                     /locus_tag="VVN16_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5573383.1"
                     /translation="EGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKS
                     TLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGIK
                     NFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKTSPFIFFDEPSSGMD
                     YSNMI"
BASE COUNT          192 a           62 c          100 g          144 t
ORIGIN      
        1 agaaggtgga gagtatcaga taaaaaatct tagctacaaa tttactgatg atgagtgttt
       61 aagcttaaaa gatatttcat tcaagcttgg gaaaatttat ggcataatag gatccaacgg
      121 acgaggaaaa tcaacgcttt taagatgttt aataggtctt gagaaaaaat caaaagaaga
      181 aatttatttt aagggagaga agctatctaa aaaagaaaga ctcaaaaact cttcacttgt
      241 tatgcaagat gtaaatcatc aattattcac agatgaggta ttcaacgagc ttagattagg
      301 aataaagaat tttgatgaag aaaaggcgaa aatcatttta aaagatttag gcctggacga
      361 atttattgaa aggcatccga tgagtttatc aggagggcaa aagcaaaggc ttgcaatagc
      421 atctgttatg tgcaagactt ctccatttat cttttttgac gaaccttcaa gtggtatgga
      481 ttattccaat atgataaa
//
LOCUS       DAVHIT010000200          491 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVHIT010000200 DAVHIT010000000
VERSION     DAVHIT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.202"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            23..307
                     /locus_tag="VVN16_002096"
     CDS             23..307
                     /locus_tag="VVN16_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573384.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            309..449
                     /locus_tag="VVN16_002097"
     CDS             309..449
                     /locus_tag="VVN16_002097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573385.1"
                     /translation="MKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVDAFM
                     AL"
BASE COUNT          175 a           80 c           85 g          151 t
ORIGIN      
        1 taaattttat aggaggaaga caatggattg gtacgattat atgatacagg catccaaaca
       61 atcacaattc aacgcaagcc attggtttcg ctatttgcga aaagttattt ttgaagacta
      121 ttcttattta acaaaccaag atgtagaaaa gttgctagac tccaaagaac taacccgttt
      181 tcaaaaaatt agcttgaagt atgcctttca agagcatact ccaactcata aatatgtgat
      241 ttcattaaat aaacctgcta agttaaccaa tgttcaaaaa ttgatggaga aatacaaaca
      301 tggataaaat gaaaccggtc ttccaagccc taaataagga attaattcag gaaaatctga
      361 ctttaacaat tatctgtgtc ggtggttatg tcttagaata tcatggttta cgtgccacac
      421 aagatgttga tgcttttatg gctctataat atttgtagtg ggtaaatccc ctatggatat
      481 tatggagcct a
//
LOCUS       DAVHIT010000201          468 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVHIT010000201 DAVHIT010000000
VERSION     DAVHIT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.203"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..262)
                     /locus_tag="VVN16_002098"
     CDS             complement(<1..262)
                     /locus_tag="VVN16_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573386.1"
                     /translation="MFYNQQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGIL"
BASE COUNT          197 a           80 c           66 g          125 t
ORIGIN      
        1 ctaaaatacc aaataccaag ccacttttca atttgtataa atagaaaatg taagataata
       61 gaaaataaaa caggactaac aaaattgtat tgactacaat agctactata aaatctaatc
      121 ctaaatcacc tggaataccc aatttactta ctatgactag taacacaatg ccattcaaag
      181 cataaataat ggaaactgag atggcatata gaaaattaga caagacgtaa atcatccgat
      241 tcttctgttg attataaaac aaacttaccg tattgtggga aaaatctcta ctaatgactc
      301 gattagcatg tataacagcc attaacatac cgagcatagc ataaaagttt gaaatatgct
      361 taataccaag cgatgtccct tgagtcttta gaataaagaa tgtaaccaat atcggcggta
      421 taaacgagat aagcaaaccg agatatatta cgcctgatga atataaat
//
LOCUS       DAVHIT010000202          465 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVHIT010000202 DAVHIT010000000
VERSION     DAVHIT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.204"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            22..>465
                     /locus_tag="VVN16_002099"
     CDS             22..>465
                     /locus_tag="VVN16_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5573387.1"
                     /translation="MGIKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVY
                     SYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQ
                     DGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADE"
BASE COUNT          170 a           54 c           98 g          143 t
ORIGIN      
        1 ggtgcaacta atgccagaaa cataggtatc aaaaattcta gaggtaagta tatatcattt
       61 ttagacgatg atgatgaata tatgccagat cgaattttaa agttgatggc ttgttttaaa
      121 aagagtagaa tgaagaattt agctttagtt tatagttatg gaataataat ttatccaaat
      181 ggtacacgag aagaggagaa gaccgatttt gttggaaatc ccttgtttgt tcaaatggtt
      241 cacaatatag caggtacgtc attttggttg tgtaaaaaag aggtgctaga attaattaat
      301 ggttttgaga aaatagattc acatcaggac ggtgttgttt tattaaaact acttgctcaa
      361 ggataccaaa ttgatatagt gcgagaattc ttggtgaatt actacgctca cagtaaagaa
      421 aacggtatca ctggagtgac acaaaaaaca attaatgcag atgaa
//
LOCUS       DAVHIT010000203          462 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVHIT010000203 DAVHIT010000000
VERSION     DAVHIT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.205"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..>462)
                     /locus_tag="VVN16_002100"
     CDS             complement(<1..>462)
                     /locus_tag="VVN16_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5573388.1"
                     /translation="CRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLER"
BASE COUNT          150 a           92 c          106 g          114 t
ORIGIN      
        1 aaccgttcta aaacaggtgt gataaagctg tctgcctctt cagaacaata acgattacca
       61 ggacgaagct gggcattgaa acaataacct gtcttcccct cgaaagcata aagaggatga
      121 tagccatgag cacgatagtg ggcgttataa gcaacacctt cttgcttgcc ataagttgtg
      181 aaatgggtag aatcgatatc tacaatgagt tggtttagct ggtgaaactg taaaaagaat
      241 tcgaccaatt caaggttgag gcatcgcaaa ctatggactg tttcctcgtc agttctggaa
      301 agaaaacggg ataaggttgg ctgtgaagca agctgccctc cttccaacaa ttttggaaag
      361 taggcatcag ctgacaattc tttacaagca tagtccgttc cataacctgt taacagttga
      421 aagaggaact ggacaaggat atctgaatcc gaataacgac ag
//
LOCUS       DAVHIT010000204          441 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVHIT010000204 DAVHIT010000000
VERSION     DAVHIT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.206"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(<1..361)
                     /locus_tag="VVN16_002101"
     CDS             complement(<1..361)
                     /locus_tag="VVN16_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5573389.1"
                     /translation="MVTIKDIARVSGVSHGTVSNVLNKKGNVSAAKIKLVEETAKKLG
                     YNMNSQAQFLRKGASSKCFFLLFNNARKKYAEYFAEIDQLSLDVEYVYLHKFSEIKNK
                     ISAILSENPQFIVCLGFS"
BASE COUNT          149 a           76 c           53 g          163 t
ORIGIN      
        1 gagaaaaacc taagcaaact ataaattgag gattttcact taaaattgca gatatcttat
       61 ttttaatctc tgagaattta tgtaagtaaa catattctac atctaaagat aactgatcaa
      121 tttctgcaaa atactcagca tactttttgc gtgcattatt aaataacaag aaaaaacatt
      181 tactagaagc gccttttcga agaaactgag cttgagaatt catattataa cccaattttt
      241 tcgcagtctc ttctactaac tttattttag ctgctgagac attacctttt ttatttaaaa
      301 cgtttgaaac tgtcccatgt gagactccag acactcttgc aatatctttt attgtaacca
      361 taattaacct ctagtttaag agatctttta gtatatttga gatctcttta cgctctttaa
      421 tttgaaatgc tttaatacct a
//
LOCUS       DAVHIT010000205          409 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVHIT010000205 DAVHIT010000000
VERSION     DAVHIT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.207"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
BASE COUNT          131 a           81 c           44 g          153 t
ORIGIN      
        1 tttctatatc ataagcttct actccaaaaa gataaagaca ctacttttgc ccttttcgat
       61 tctcttttta aacatagcat catcttcctt cattcctata aaaacatttc cgatgagaga
      121 gcttattttt taatttcgtt aaacaacaaa aaacgcaagc ttaagcctgc ggacaatctt
      181 tatagccatt tcatttcttt cacatctgtt ttacgaactt tagcatattt cttagcgctt
      241 gtaaaatgta gagtaactgg tttaaatact ccgcttttat ctcttcgtaa acctaaaact
      301 gcggatcttt tatttttatc ctcaaaaaat atgacgtcca atttcatagt atttttagat
      361 aaatccctag ctacgatgca acaacttatt attttagaat agaaaatat
//
LOCUS       DAVHIT010000206          392 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVHIT010000206 DAVHIT010000000
VERSION     DAVHIT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.208"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            28..>392
                     /locus_tag="VVN16_002102"
     CDS             28..>392
                     /locus_tag="VVN16_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU5573390.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QAAQKTENLTPDEVSKKEGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEI"
BASE COUNT          153 a           64 c           79 g           96 t
ORIGIN      
        1 caaatatact taagaaaaga ggaaagaatg aaaatcaata aaaaatatct agctgggtca
       61 gtagctacac ttgttttaag tgtctgtgct tatgaactag gtttgcatca agctcaaact
      121 gtaaaagaaa ataatcgtgt ttcctatata gatggtaaac aagcagcaca aaaaacggag
      181 aatctgactc ctgatgaggt cagtaaaaaa gaaggtatca atgccgaaca aatcgtcatc
      241 aagataacag accaaggcta tgtgacttca catggggatc actatcatta ttacaatggg
      301 aaggttcctt atgacgctat catcagtgaa gaattgctga tgaaggatcc aaactaccag
      361 ctaaaggacg aggatattat cagtgaaatc aa
//
LOCUS       DAVHIT010000207          355 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVHIT010000207 DAVHIT010000000
VERSION     DAVHIT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.209"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            188..>355
                     /locus_tag="VVN16_002103"
     CDS             188..>355
                     /locus_tag="VVN16_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573391.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSYKRTHIN"
BASE COUNT          185 a           50 c           57 g           63 t
ORIGIN      
        1 cagggagaaa aaccaagtgt aatagcacag attaaaaagt tccaagacca agtcaataaa
       61 gataatgaat acagagaaat gatgaaaaac aagaaaatgg atcaagaaag ataagacctg
      121 gtaaaaatat cttatccaag ctataatatg accaaacaac taaagtcaga tctaggaggt
      181 aatagagatg aataaaaggc aagagctaat agatgaactc ataaaagcaa atgaagatgg
      241 aacatacaaa acctataaaa gcacagaaga aataaaagca atgaacaatg aagaaataca
      301 gattatatat tccaacatga aaaactatct atcatacaaa agaacacaca taaac
//
LOCUS       DAVHIT010000208          322 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVHIT010000208 DAVHIT010000000
VERSION     DAVHIT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.denovo.210"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            <1..>322
                     /locus_tag="VVN16_002104"
     CDS             <1..>322
                     /locus_tag="VVN16_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5573392.1"
                     /translation="FDQVASLLEEREIVTPTYREALITREKSFPTGLDMEFLGKDLPN
                     VAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNILA
                     QLMD"
BASE COUNT          101 a           58 c           64 g           99 t
ORIGIN      
        1 tctttgatca ggtagcaagc ttgttggaag aacgagaaat tgtcactcca acttatcgtg
       61 aagccttgat cacgcgtgaa aagtcatttc caactggttt agatatggaa tttctaggaa
      121 aggacttgcc aaatgtagcg attcctcaca cagatattgt tcataatcta gctgagaaag
      181 tggtggttgt tcgattagaa aaaccagtaa cttttcacaa tatgatagct cctgataagg
      241 aagtagaagt atctctactc ttctttatca ttaacaattc aagttcaagt caaacgaaca
      301 ttctggctca gttgatggac tt
//
LOCUS       DAVHIT010000209        19459 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.guided.033, whole genome shotgun sequence.
ACCESSION   DAVHIT010000209 DAVHIT010000000
VERSION     DAVHIT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.guided.033"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            complement(282..734)
                     /locus_tag="VVN16_002105"
     CDS             complement(282..734)
                     /locus_tag="VVN16_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573393.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVRSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     TKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDAIDEVKSQITFK"
     gene            complement(724..1023)
                     /locus_tag="VVN16_002106"
     CDS             complement(724..1023)
                     /locus_tag="VVN16_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573394.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIFDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            1698..2012
                     /locus_tag="VVN16_002107"
     CDS             1698..2012
                     /locus_tag="VVN16_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5573395.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2028..2414
                     /locus_tag="VVN16_002108"
     CDS             2028..2414
                     /locus_tag="VVN16_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5573396.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            2443..3828
                     /locus_tag="VVN16_002109"
     CDS             2443..3828
                     /locus_tag="VVN16_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5573397.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3831..3983
                     /locus_tag="VVN16_002110"
     CDS             3831..3983
                     /locus_tag="VVN16_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5573398.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4006..5210
                     /locus_tag="VVN16_002111"
                     /pseudo
     CDS             4006..5210
                     /locus_tag="VVN16_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            5253..5474
                     /locus_tag="VVN16_002112"
     CDS             5253..5474
                     /locus_tag="VVN16_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573399.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            5591..6088
                     /locus_tag="VVN16_002113"
     CDS             5591..6088
                     /locus_tag="VVN16_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU5573400.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            6063..6569
                     /locus_tag="VVN16_002114"
     CDS             6063..6569
                     /locus_tag="VVN16_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5573401.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            6553..9000
                     /locus_tag="VVN16_002115"
     CDS             6553..9000
                     /locus_tag="VVN16_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5573402.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            9003..11180
                     /locus_tag="VVN16_002116"
     CDS             9003..11180
                     /locus_tag="VVN16_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5573403.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDRVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            11177..12178
                     /locus_tag="VVN16_002117"
     CDS             11177..12178
                     /locus_tag="VVN16_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU5573404.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            12175..13107
                     /locus_tag="VVN16_002118"
     CDS             12175..13107
                     /locus_tag="VVN16_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5573405.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            13359..15278
                     /gene="tet(M)"
                     /locus_tag="VVN16_002119"
     CDS             13359..15278
                     /gene="tet(M)"
                     /locus_tag="VVN16_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU5573406.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEILDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            15397..15564
                     /locus_tag="VVN16_002120"
     CDS             15397..15564
                     /locus_tag="VVN16_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU5573407.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(15624..15977)
                     /locus_tag="VVN16_002121"
     CDS             complement(15624..15977)
                     /locus_tag="VVN16_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5573408.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            16482..16904
                     /locus_tag="VVN16_002122"
     CDS             16482..16904
                     /locus_tag="VVN16_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5573409.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            16901..17131
                     /locus_tag="VVN16_002123"
     CDS             16901..17131
                     /locus_tag="VVN16_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5573410.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            17592..17795
                     /locus_tag="VVN16_002124"
     CDS             17592..17795
                     /locus_tag="VVN16_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU5573411.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            17877..19094
                     /locus_tag="VVN16_002125"
     CDS             17877..19094
                     /locus_tag="VVN16_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5573412.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(19271..>19459)
                     /locus_tag="VVN16_002126"
     CDS             complement(19271..>19459)
                     /locus_tag="VVN16_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5573413.1"
                     /translation="DIRVALTSVLFALVHHPGTILAWCLYVSLGIFLGMVRYKSDLWG
                     SMGLHLVWNLLVYSLLLF"
BASE COUNT         6397 a         3519 c         3962 g         5581 t
ORIGIN      
        1 tccatcgtta atcggagatg gactagaatg ctttttgaaa acagatgagt attagaaata
       61 aatgaaaagt caatatcaaa acaatcagaa tcttgatgat gctaaattca aaaaaactac
      121 ataataattt taataagggt aagtcgtacc aagaaacccc accaagataa aaacttagtg
      181 gggtttggcg gggaatataa ttattctccc aattattatc gcctttatat tatctcaact
      241 ctcattaaac aagagttccg caatcaaatt gcgatactct attatttaaa ggtaatttgg
      301 gatttaacct catcaattgc atcgtctaat gacttatcca tggaaaaatc agataatttc
      361 tcaggatcat tcaccatttt ttctaacaga tggtctaaat gattgtctag aatcgaacgg
      421 tctttcgtgt agaaatgcag ggaatcccct tgccaagcga tggctaaagg aatctcttct
      481 ttttctaaaa aagctttaaa tttctctata tcaattggtt tgtctaaaaa atcttttttc
      541 agattaatcg tgtcaatcga ataaggagat tgtagcaatt cttctaattt ctgcacccct
      601 atcttatagg cggaatcctg tgctaaagcc tgacgtctag accattctag aatctttaag
      661 agacttctta cagtaaataa aagactacgc tctgcatatt gaactgccat tcgttcctgt
      721 tgttcagagg acatctgagg cctccttctt cacaaatagc agctttcctt ctttataacg
      781 ataagctatc aatttctgac gttgcttaat cgacttgaca acctgcagta gatctctcga
      841 ataaggttct cgaagagtaa cttctactgc ttttccatca aagataccaa cacgtttaaa
      901 ttcaatgtag tcttttttga gatgtcctaa gcccatacca aaaggaaagt gatgaatcaa
      961 ttggatggta caaccacctt tatttcccat ttgtttcaaa tcatacaagt ttactacctt
     1021 catgattaac tcctttatct agtttgtcgc atcgtttaag tctggtaacg ataaactccg
     1081 tgtctgttag aaaattctca cacacgtctt gtgccagttg cccttcacag ggaaatactc
     1141 tcagtcccta cttacacagg cacgctaatc aagacggagt gaattcaatt ttcaaagaac
     1201 aggtagcttt attatagata agagtagttg aaatttttat cacatttttt attttcctgt
     1261 agagctaaat tcatactaaa caaagatttt gaaataaaag actaacattg ttacataacg
     1321 ttagcatttc atttccctaa aataagctaa tttttaccag aaaaaatagc ataaaaatct
     1381 agttatccgc ataaaaactg gacttatcac actttatcaa ggtcaaaacc actcaattta
     1441 ctactaattt actacttatg aatgagcttt gatacgacga tttatccttg aaaagtgaag
     1501 atataaagat acttccaata aaatttgaat atttaatagg tagacacttc aaaaaatgag
     1561 gtgtctattt ttttacccga ttttgaaagg aagtgaactt atgaaaacaa aaaatcaaga
     1621 atcaaaaggt cgttccccac tctttaagac catcaaacat tcattcagcc aataaaaaag
     1681 aaaggatagg taaaaatatg gaacttaaat ttgtgattcc caacatggaa aaaacattcg
     1741 gcaatttaga atttgctggc gaggataaag tcgttcagcg aagaatcaac ggacggctaa
     1801 ctgtcttatc aagaagctat aatctctatt ctgatgttca aagagcagat gatattgtgg
     1861 tggtgcttcc tgctgaagct ggcgaaaaac atttcggctt tgaggaacgt gtgaagttag
     1921 tcaatccacg tattaccgca gagggctaca aaatcggcac tcgtggtttt acaaattacc
     1981 ttttacatgc tgacgacatg ataaaagaat aaagaaagag aggaaaaatg atgagattag
     2041 caaatggcat tgtattagat aaagacacga cttttggaga attgaaattc tctgctctac
     2101 gtcgtgaagt gagaatccaa aatgaagacg ggtcggtttc agatgaaatc aaggaacgta
     2161 cctatgactt aaaatccaaa ggacaaggac gcatgattca agtaagtatt cctgccagcg
     2221 tgcctttgaa agagtttgat tataacgcac gggtggaact tatcaatccc attgcggaca
     2281 ccgttgctac tgccacctat caaggagcag atgttgactg gtatatcaag gcagacgata
     2341 ttgtgctgac aaaggattct agttcattca aagctcaacc acaagcaaag aaagaaccga
     2401 cacaagacaa atagtcgcta ggtagaaagg agactttttc gcatgaaaca gcgtggtaaa
     2461 aggattcgcc catctggtaa agatttagtc tttcatttta cgatagcgtc actcctgcct
     2521 gttttcctgc tggttgtcgg actgtttcat gtgaagacaa tccagcagat caactggcag
     2581 gattttaacc tatcacaagc agataagatt gacattccct atttaattat cagtttcagt
     2641 gtcgcaattc ttatctgctt gctggtagcg tttgtattca aacgggttcg ctatgatacg
     2701 gttaaacaac tttaccaccg tcaaaaactg gcaaagatga tacttgaaaa caagtggtat
     2761 gaatctgaac aggtcaaaac agagggtttc tttaaagata gtgctggtcg tacaaaggaa
     2821 aagataacct acttccctaa aatgtattat cgacttaaaa atggcttgat acagatacgg
     2881 gtggaaatca cgctgggaaa atatcaagac caactcttac acttggaaaa gaaattagag
     2941 agtggcttgt actgtgagct gacggataaa gagttaaagg attcctatgt ggaatatact
     3001 ttgctctatg acaccatagc cagtcgtatt tctattgatg aagtagaagc taaagatggt
     3061 aaacttcgct taatgaaaaa cgtatggtgg gaatatgata agctccctca tatgttgatt
     3121 gctggtggta caggtggcgg taaaacttac tttatactga cactgattga agccttgctt
     3181 catacagatt caaaactgta tattcttgac ccgaaaaatg ctgatcttgc ggacttaggt
     3241 tctgtgatgg caaatgtcta ctatagaaaa gaagacttgc tttcttgcat tgaaacattc
     3301 tatgaagaaa tgatgaaacg tagtgaggaa atgaagcaga tgaagaacta taagactggc
     3361 aaaaattatg cttacttagg tctcccggca cacttcttaa tctttgatga atacgtcgct
     3421 ttcatggaaa tgctgggaac aaaagaaaac accgcagtta tgaataagct gaaacagatt
     3481 gtcatgttag gtcgtcaagc tggcttcttt ctaatactgg cttgtcaacg tccagacgca
     3541 aaatatttag gcgacggaat ccgtgatcag tttaatttca gagtggcttt aggtcgtatg
     3601 tctgaaatgg gctatggcat gatgtttggc agtgacgtac aaaaggattt cttcttaaag
     3661 cgaatcaaag gtcgtggcta tgttgatgta ggaacaagtg tcatatcaga gttttatact
     3721 ccccttgtac caaaaggata tgatttcttg gaggaaatta aaaagttatc caacagcaga
     3781 cagtccacgc aggcgacgtg cgaagcggaa gtcgcaggtg tggactgatc ttgctggctg
     3841 gtgtggcaat agccacgcca gcacttaacc ccccgtatct aacagggggg tacaaatcga
     3901 caggaaacag tcaaaaaaac attagaaaat cctttggtta caagggattt acaaaatttc
     3961 agcgtatgtc aaatgggctt taaaagttga catacgcctt tttgattgga gggattttta
     4021 ctgaatgaac aaacttggtt acagcattta aaagaaaaac gcttggctta tggactatct
     4081 caaaaccgtt tagctgttgc gactggtatt acaaggcagt atctaagcga tattgaaaca
     4141 ggaaaagtca agccatcaga ggatttacag cagtcccttt gggaagctct ggaacgcttc
     4201 aatcccgacg ctccccttga aatgctgttt gattatgtaa gaattcgctt tccgacaaca
     4261 gacgtacagc aggtggtcga aaacatctta caactgaaac tgtcctattt tcttcatgag
     4321 gactatggtt tctattctta ttcagagcat tatgctttag gcgacatatt cgtcctttgc
     4381 tcccatgaac tggacaaagg agttctggtg gaattgaaag gtcgtgggtg cagacaattt
     4441 gaaagctatc ttctggcaca acaaagaagc tggtatgagt tctttatgga cgttttggtg
     4501 gctggcggtg tgatgaaacg ccttgacctt gccattaacg ataagacagg gattttaaat
     4561 atccctgtac tcactgaaaa gtgccaacag gaagaatgta tctccgtctt ccgcagtttt
     4621 aaaagctatc gcagtggcga actggtacgc aaagaggaaa aggaatgtat gggaaacacc
     4681 ctctatatcg gttcattaca aagtgaagtt tatttctgta tctatgaaaa ggactacgag
     4741 cagtacaaga aaaatgatat tcccattgaa gacgcagaag taaaaaaccg ttttgagatt
     4801 cgattgaaaa atgagcgtgc tattatgcag tccgtgattt actcgtctat gacaatccag
     4861 agcataccgc ctttaaaatt atcaatcggt atatccgttt tgtagataaa gacgattcca
     4921 aacctcgttc tgattggaaa ctgaatgaag aatgggcttg gtttattggg aacaatcgtg
     4981 aacgattaaa actaaccaca aaaccagagc cttactcctt ccaaaggacg ctgaactggc
     5041 tatctcatca agttgccccg accttaaagg ttgcgattaa acttgatgaa atcaaccaga
     5101 cgcaggttgt aaaagacatt ctcgaccatg cgaaactgac agaccgacac aagcagattt
     5161 tgaagcaaca gtcagtaaaa gaacaggacg tgataacaac aaaaaaataa ctcaaataca
     5221 aattcattga atatagagag gagaacattt ttatgaattt tggacaaaac ctttataact
     5281 ggtttctatc aaacgctcaa tcactggtgc ttttagcaat cgttgtgatt ggcttgtatc
     5341 ttggcttcaa gcgtgagttt agcaaactga ttggcttttt aattattgcg attattgcgg
     5401 ttggcttagt cttcaacgct gctggagtaa aagacatttt actagagcta ttcaatcgca
     5461 ttattggtgc ttaaataaaa ccgttctttt gtggaatata agtggttttc ttatgttccg
     5521 caaaggaatg gtacaccaaa cgaagtgcgg tagggatttt tgaatctcta caaagaaagg
     5581 acgtgaatat atggacgata tgcaagtcta tattgcgaat ttaggcaaat acaatgaggg
     5641 cgaattggtc ggtgcgtggt ttacctttcc cattgacttt gaggaagtca aagagaaaat
     5701 cggcttgaat gatgaatatg aggaatacgc cattcatgac tacgagttac cctttacggt
     5761 tgacgaatac acttccattg gcgaactcaa tcgactatgg gaaatggtat cggaattacc
     5821 cgaagaatta caatcggagc tatctgctct gctcactcat ttttcaagca ttgaagaact
     5881 aagcgaacat caagaggata ttatcattca ttccgattgt gatgatatgt atgacgtggc
     5941 acgctactac attgaagaaa cgggtgcttt aggcgaagta ccagctagtc ttcaaaacta
     6001 tattgattat caagcctatg gtcgggattt agacctttca ggaacgttta tctcaaccaa
     6061 tcatgggatt tttgaaatcg tctattaaat ctgtcggtac attactactg gcagattttc
     6121 tattttacgg ggtggctcaa tcagctaccc ctatttttta tgaaaggatt gattacatga
     6181 agaaaatacg aagctatacc agtatctggt ctgtggaaaa ggtactgtat tctatcaatg
     6241 attttagact tccgtttccc ataaccttta cgcaaatgac atggtttgtc gtgtcactct
     6301 ttgcagtgat gatacttggc aacttgcccc ctctttccat gatagaggga gcatttctca
     6361 aatactttgg gattcctgtg gctttcacat ggtttatgtc tacaaaaact tttgatggta
     6421 aaaagcctta tggatttttg aagtctgtca ttgcttatgc actgcgacca aagctgacct
     6481 atgcaggaaa aaaagtaacg cttggcagaa accagccaca agaagccatt acagcagtta
     6541 ggagtgaatt ttatggcata tccaattaaa tacattgaaa acaatctcgt ctggaataaa
     6601 gacggggaat gttatgctta ctatgagctt gttccttaca attactcatt tctaagtcca
     6661 gaacagaaaa tacaagtgca tgattctttc agacagctta tcgcacaaaa tcgtgatggc
     6721 aaaattcatg ctttacaaat cagtacagaa tccagcatac gttctgcaca agagcgttcc
     6781 aaaaatgaag tcactggcaa gctcaaagcg gttgcctatg acaaaatcga ccaacagaca
     6841 gacgctttaa tatccatgat tggcgaaaat caagtgaact accgtttctt tatcggcttt
     6901 aagttgcttc tcaacgatca ggagttttct atgaaaagtc ttaccgttga agcaaaaaat
     6961 gctttgtctg attttgtcta tgatgtgaac cataagctga tgggcgattt tgttagtatg
     7021 agtaatgatg aaatcctgcg ttttcagaag atggaaaagc tcttagaaaa taaaatctct
     7081 cgtcgtttca aaatccgcag gttagataag gacgacttcg gctatctgat tgaacacctt
     7141 tacggacaga caggcactgc ctatgaagag tatgagtacc atctatcaaa gaaaaagctg
     7201 gataatgaaa cgctgattaa atactatgac ttgattaagc ctactcgctg tttggtggaa
     7261 gaaaaacagc gatatttgaa aatccagcag gaagatgaaa ccgtctatgt agcttacttt
     7321 accattaaca gcattgtcgg agaactggac ttcccgtcct ctgaaatctt ctactaccag
     7381 caacagcaat ttacattccc gattgatacg tcaatgaatg tggaaattgt agcgaatcgt
     7441 aaagccctat ctactgtccg caataaaaag aaagaactga aagacttgga taaccacgct
     7501 tggcaaagtg ataatgaaac cagctccaat gtggcggaag ctctggaaag tgtgaatgag
     7561 ctggaaacca atttagacca aagcaaggaa tctatgtaca agctgtctta tgtggtaagg
     7621 gtatcagcaa atgatcttga cgaactcaaa cgtcgttgta atgaagtgaa agatttttat
     7681 gacgatttaa gcgtaaaact ggtacgacca tttggggata tgctcggctt acatgaagaa
     7741 tttttacctg ccagcaagcg ttatatgaat gattatattc aatacgtgac ctctgatttc
     7801 ctcgctggtt taggttttgg tgctactcaa atgctggggg aaaatgaggg gatttatgtt
     7861 ggctacagct tagatactgg acgcaatgtc tatctgaaac ctgctcttgc cagtcaaggg
     7921 gttaagggtt cagtaaccaa tgcgttagcg tcggcttttg ttggttcgct gggtggtggt
     7981 aaatcctttg cgaataacct tatcgtctat tatgcggtgc tttatggggc acaagcagtg
     8041 attgtagacc caaaagcaga acgtggcaga tggaaagaaa ccttgccaga gatttcccat
     8101 gaaatcaata tcgtcactct gacttctgat gagaaaaaca aaggcttact tgacccttat
     8161 gtgattatga aaaatcccaa agattctgaa tcactggcta ttgatattct gacattcctt
     8221 acggggattt cctctcgtga tggggaacgc ttcccaatcc ttagaaaagc cattcgtgca
     8281 gtaaccaata gtgaagtacg agggttgatg aaagtgattg aggaattacg ggttgagaat
     8341 acgccactaa gtaccagtat agccgaccat atcgaaagtt ttacagacta tgactttgca
     8401 catttattat tcagtaatgg ttatgtggag cagtctatca gcttagaaaa acaactgaac
     8461 attatacagg ttgcggactt ggtacttccc gacaaggaaa cttcctttga ggaatatacc
     8521 actatggagc ttttatccgt tgctatgctg attgtcatta gtacctttgc tttagacttt
     8581 atccatacag accgaagcat tttcaagatt gtagatttag acgaagcatg gagcttttta
     8641 caggtagcac aaggaaaaac actatctatg aagctggttc gggctggtcg tgctatgaac
     8701 gctggggtat atttcgtgac ccaaaataca gacgacctct tagatgaaaa actgaaaaat
     8761 aacctcggct taaaatttgc atttcgttcc actgacctta acgagattaa aaagacctta
     8821 gccttttttg gtgtagaccc agaggacgaa aacaatcaga agcgattgcg tgatttggaa
     8881 aacgggcaat gccttatcag tgatttatat ggtcgtgtcg gtgtgataca gttccaccct
     8941 gtatttgaag aactgctcca tgcctttgat accagaccac ctgtgcgaaa agaggtgtaa
     9001 atgtgaaacc atcaatagta aacagaataa aatcaaactg gacgctgaaa cgtctaggta
     9061 aagtggcaat gacagtggct ttcacacttg tgattgccat ttttctttta gccatgctgg
     9121 gaacggtggt tcaagctgcg ggcttggtag atgatacggt caatgtggca aatgaataca
     9181 gccgataccc acttgaaaac tatcaactgg atttttatgt ggataatagc tggggctggc
     9241 ttccgtggaa ctggtcggac gggattggaa aacaggtcat gtatggacta tatgccatta
     9301 ccaattttat ttggacaatc agtttgtatg tttccaatgc gacaggttac ttagtacagg
     9361 aagcctattc cttagacttc atttccgcta cagcagattc cattggtaag aatatgcaga
     9421 ccttagctgg tgtgagtgca aacggatttt caacagaggg tttctatgtt ggattcctct
     9481 tactcttgat tttggttctt ggggtttatg ttgcctatac gggactgata aagagagaaa
     9541 ccacaaaggc aattcatgcc attatgaatt ttgtgctggt gtttatccta tcggcttcct
     9601 ttattgccta cgctcccgac tacattaaaa aaatcaatga cttttcatca gacatcagta
     9661 atgccagttt atcacttggc acgaagattg tcatgcccca ttccgatagt caaggcaagg
     9721 acagagtgga cttaatcaga gatagcctgt tttccataca ggttcagcaa ccgtggctac
     9781 tgcttcaata caacagttca gacattgaaa gtatcggtat tgaccgtgtg gaaagcctgc
     9841 tctccaccag cccagattcc aacaatggcg aagacagaga aaaaattgtt gcggaagaaa
     9901 ttgaagacag aagcaatacc aatctaacca ttacaaagac cattaaccgt ttaggtacag
     9961 tcttcttcct atttgtcttc aatattggga tttccatatt tgtattccta ttaacaggaa
    10021 tcatgatttt ctcgcaggta ctttttatca tctatgctat gtttctgcct gtgagcttta
    10081 ttttaagcat gattccatca tttgatggta tgtcaaaacg agccataaca aagctcttta
    10141 ataccatttt gacacgagct ggaatcacat tgattattac gacagcattt agtatttcaa
    10201 ccatgctcta taccttatcg gctggttatc cgttcttttt gattgctttt ctacagattg
    10261 tgacctttgc aggaatctac ttcaagctgg gcgatttaat gagtatgttt tctctacaga
    10321 gtaacgattc tcaaagtgtg ggaagtcgtg tgatgagaaa acctcgtatg cttatgcacg
    10381 ctcacatgca ccgtctacag cggaaacttg gacgttccat gactactcta ggggctgggt
    10441 ctgccattgt tacaggtaaa aaaggacagt cgggttcggg gagttctgca aggacacaag
    10501 cagatcactc ccgaccagac ggaaaggaaa aatcaacact tggaaaacgt atcggtcaaa
    10561 ccatcggtac agtagctgat accaaagaca gaatggtaga cactgctagt ggtttgaaag
    10621 aacaggttaa agatttgccg accaatgcaa gatatgcagt atatcaagga aaatccaaag
    10681 taaaagagaa tgtccgtgat ttaaccagta gtatttctca aaccaaagcg gacagagcca
    10741 gtggacgcaa ggaacagcag gaacaaaggc gaaaaaccat tgcgaagcgt cgctctgaaa
    10801 tggaacaggt caaacagaaa aaacagcctg cttcttctgt tcatgaaaga ccgactacaa
    10861 gacaagaaca atatcatgat gaacagacct caaaacagtc taatattcag acttcatata
    10921 aggaatctca acaagccaaa caagagcgtc cagcagttaa gtccgatttt tcaagtccaa
    10981 aagtggaacg ccaaggcaat accgttcaag aaaaaaccgt tcaaaagcca gcaacttcaa
    11041 ccactacagc agatagaact tcacaacgtc caatcacaaa agaacgtccg tctactgttc
    11101 aaagagtacc actacaaaat acaagaagta gaccaccaat caaaaccgcc accattaaga
    11161 aagtcggtaa gaaaccatga agttgaaaac tttagtgatt ggtggttctg gattattctt
    11221 gatggtcttc tcactgcttc tgtttgttgc cattttattt tcagatgaac aggacagcgg
    11281 aatttccaat attcattatg gaggtgtgaa tgtttccgca gaagtgctgg ctcataagcc
    11341 tatggtagaa aaatatgcca aagaatatgg cgttgaagaa tatgtcaaca tacttcttgc
    11401 gattatacag gtggaatcgg gcggtactgc ggaagatgtt atgcagtcct cggaatccct
    11461 cggtcttcca cctaattcat tgagtacaga agaatccatt aagcaaggtg tgaagtattt
    11521 cagtgaatta ttagccagta gcgaaaggct cagtgtagat ttagaatcgg ttatccagtc
    11581 ctacaattat ggtggtggtt tcttagggta tgtggctaat cgtggaaata aatatacctt
    11641 tgaactggct caaagtttct caaaagagta ttcaggtggc gaaaaagtgt cttaccccaa
    11701 tcccatagcc atacctatca atgggggctg gcgatacaac tatggcaata tgttttatgt
    11761 gcaacttgta acgcagtatc ttgtcacaac agagtttgat gatgatacgg tacaagccat
    11821 catggacgaa gcactgaaat atgagggctg gcgatacgtt tacggtggag cttccccgac
    11881 tacttctttt gattgtagcg gactgacaca atggacgtat ggaaaagctg gaattaactt
    11941 accacgaacc gcacaacagc aatatgatgt gacccagcat atcccactat cggaagcaca
    12001 agctggcgat ttggttttct ttcattctac ctataacgct ggctcttata ttactcatgt
    12061 tgggatatac cttggcaata accgtatgtt tcatgcaggc gacccaatcg gttatgccga
    12121 cttaacaagc ccctactggc aacagcattt agtgggagca ggacgaatca aacaatgaga
    12181 aaggaagatt taatgatgaa atttagaaaa aatcagaata aagaaaaaca gataccaaag
    12241 gaaaagaaac ctcgtgtcta taaggtcaat cctcataaaa aggttgtgat tgccttgtgg
    12301 gtacttttag ggcttagttt cagctttgcg atattcaagc actttacagc tatagatact
    12361 catactattc acgaaacaac tatcatagaa aaggaatacg ttgatactca tcatgtagaa
    12421 aattttgtag agaactttgc gaaagtctac tattcatggg agcaatccga taagtccatt
    12481 gataatcgaa tggaaagtct aaaaggctat ctgacagatg aacttcaagc tctcaatgtt
    12541 gatacagtac gcaaagatat tcctgtatcg tcttctgtaa gaggatttca gatatggacg
    12601 gtagagccaa ctggcgacaa tgagtttaat gtaacctaca gtgtagacca gctcattaca
    12661 gagggagaaa atacaaagac cgtccactct gcttatatag tgagtgtcta tgtagatggt
    12721 tctggaaata tggtactggt taagaatccg accattacta acatacctaa gaaatcaagt
    12781 tataaaccaa aagccattga aagtgagggg acggttgatt ccattacaac caatgaaatc
    12841 aatgagtttt taacgacgtt cttcaagctc tatcctacag cgacagccag tgaactttcc
    12901 tactatgtga atgacgggat attaaaacca atcggaaaag agtacatctt tcaagaactg
    12961 gtaaatccta ttcacaatcg taaggataat caagtcacgg tatcgctgac agtggagtat
    13021 atcgaccagc agaccaaagc aacgcaggta tctcaatttg atttggtact tgaaaagaac
    13081 gggagtaatt ggaagattgt aaaataacaa atattggtac atgattacag atactttgta
    13141 atcatgtact ctttttgata aaaaattgga gattccttta caaatatgct cttacgtgct
    13201 attatttaag tgactattta aaaggagtta ataaatatgc ggcaaggtat tcttaaataa
    13261 actgtcaatt tgatagcggg aacaaataat tagatgtcct tttttaggag ggcttagttt
    13321 tttatgccct tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag
    13381 ttttagctca tgttgatgca ggaaaaacta ccttaacaga aagcttatta tataacagtg
    13441 gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt
    13501 tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata
    13561 cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt
    13621 cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac
    13681 aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca
    13741 ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac
    13801 tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga
    13861 actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga
    13921 aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa agcataagat
    13981 ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaagtaat atagggattg
    14041 ataaccttat agaagttatt actaataaat tttattcatc aacacatcga ggtccgtctg
    14101 aactttgcgg aaatgttttc aaaattgaat atacaaaaaa aagacaacgt cttgcatata
    14161 tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa
    14221 aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag
    14281 cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg
    14341 gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa
    14401 caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa
    14461 tcttagatag tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac
    14521 tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc
    14581 atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg
    14641 cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat
    14701 ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat
    14761 acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggc tgtgaacaag
    14821 gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc ttatactata
    14881 gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct
    14941 taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac
    15001 aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca
    15061 ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaag
    15121 aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta acagagttaa
    15181 aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga
    15241 tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt tgttatataa
    15301 atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa ttaaagtgta
    15361 aaggaggaga tagttattat aaactacaag tggatattgt gtcctgtatg tggaaataaa
    15421 acacgattaa agataaggga agatactgaa ttaaaaaaat tccccctcta ttgtccgaaa
    15481 tgcagacaag aaaatttaat tgaaataaag cagttcaaag taactgtgat tacagagcca
    15541 gacgcaaaga cgcagagccg ataaaatgag attaatacaa tctcatttta tcggctcttt
    15601 ccgttatgta tggattcttt taattagtct tcgatgtttc ttgcttcgtt gataccgctg
    15661 gctaaagatt ccattaagga tagttctttg tctgtaaagc tatccatgta tttctctatc
    15721 tgtaatcgtc gggtgctttt taccaagtta ttagcaggta agaaaaattc atcaacggaa
    15781 acatgaagta acgatacaag gtcataaaga acttgtatgc tggggtgttg ccctttattt
    15841 tcaatattag ttaagtaccg tgggtcaatt tcaatcaatg ctcccacttg ttcacgagtt
    15901 aaacctcgtt tcaatcgagc ttctttaatg gctaaaccaa aggctctaaa atcatattta
    15961 tcttcttttt tacgcatagt agaccacctc tatacatttt attgttccta ctgaattaaa
    16021 aacaggtata gaaaaacgtg ttatatggtt tataggttta tatttaataa aaagcactac
    16081 taaacgccaa taaaaaaaac cgttatatgg tagtgctatt tacgctgtta aaatattgta
    16141 tattacttcc aaatggcggt ttgttggagg tcaacgtcgc catgaagtac atcatataca
    16201 ataaatttcc ttacattggg ttcttgtcaa aaaaagtcgt ctatctgcaa tagataagta
    16261 cgtccaccaa tgtggtttta taaatcatat agatagaata acagaagcat gtaaacagag
    16321 aaataaatct gtttatatgc ttttttggct attcagaact tttttacaaa gtttatttat
    16381 cagtaatgca acaaatcccc ctttcacatt gggactaaga gtgaaaggag ataaacgagc
    16441 aaggctcact tcctttccta gacagaaagg gggtgagaaa catgaaacca tcttcttttc
    16501 agaccacaat agaaaatcag tttgactata tctgtaaacg tgctatggaa gacgagcgaa
    16561 agaattatat gctttatctt tcaaggattg caaagcgtga ggtgtccttt tcggatgttg
    16621 gcgattatct tgttagccag tttgcgacaa cagataacta ttcaactgac tttcagattt
    16681 ttacactcaa tgggttatca gtaggcgttg aaaatgattt gttgagtgaa gcattacgtg
    16741 agttgccaga caagaaacgt gaaattctac tgctgtttta ctttatggac atgagcgatt
    16801 cagaaattgc agacctgttg aaattgaacc gttctactgt ctatcggcat agaaccagtg
    16861 gactagcctt aattaaaaag tttatggagg aatttgaaga atgaaaacac aatatcctat
    16921 gattcccttt cctctcattg taaaggcaac agatggcgat accgaagcga ttaaccagat
    16981 tctacatcat tacagagggt acataacgaa gcgttcccta cgacttatga aagatgaata
    17041 tggcaatcaa agtatggtcg ttgatgaagt cttacgtgga agaatggaaa ccagactgat
    17101 tacaaagatt ttgtcatttg aaattaagta atatcctctc tcctttcgtg gaagcgtgct
    17161 aaaccattcc acgcttcccg aacagggagg tttgttattc caccaaagca tattgagctt
    17221 tcaatgtgtt ttgataggct aacgagccat tgttctttga aaactgaata aaagtaatcg
    17281 aatacgtttc gataagaaaa gagccaacgg aactaaccgc catgacctat cttataaaga
    17341 tagcgagcga ttcatgttag tgatccgaga agcaatcttt agcaggattg cctgcaacga
    17401 cattcttatc gtgataatga tactcccata cagtcaatag tccgagcgtg ataaaaccgt
    17461 cgcaggcaat gagtatggct acatgagaac catgcagggg tggaactccc gtgagctttg
    17521 ctaaagctgt tcgattgctg gtaaaacaac ttttatgaaa tccaaataag tgatttggaa
    17581 aggaggattt tatgaagcag actgacattc ctatttggga acgttatacc ctaaccattg
    17641 aagaagcgtc aaaatatttt cgtattggcg aaaacaagct acgacgcttg gcagaggaaa
    17701 ataaaaatgc aaattggctg attatgaatg gcaatcgtat tcagattaaa cgaaaacaat
    17761 ttgaaaaaat tatagataca ttggacgcaa tctagcgtcg ccaaagggtc ttgtatatga
    17821 taaaatagta ttaagtcgta tcaaggctct ttccataaag gaaaggagca aatgccatgt
    17881 cagaaaaaag acgtgacaat aaaggtcgaa tcttaaagac tggagagagc caacgaaaag
    17941 acggaagata cttatacaaa tatatagatt catttggaga accgcaattt gtttactcgt
    18001 ggaaacttgt ggctacagac cgagtaccag caggaaagcg tgattgtatc tcacttagag
    18061 agaaaatcgc agagttacag aaagacattc atgatggtat tgatgttgta ggaaagaaaa
    18121 tgacactctg ccagctttac gcaaaacaga acgctcaaag accaaaggtt agaaaaaaca
    18181 ctgaaactgg acgcaaatat cttatggata ttttgaagaa agacaagtta ggtgtaagaa
    18241 gtattgacag tattaagcca tcagacgcta aagaatgggc tattagaatg agtgaaaatg
    18301 gttatgctta tcaaaccatc aataactaca aacgttcttt aaaggcttca ttctatattg
    18361 ctatacaaga tgattgtgtt cggaagaatc catttgactt tcaactgaaa gcagttcttg
    18421 atgatgatac tgtccctaag accgtactaa cagaagaaca ggaagaaaaa ctgttagcct
    18481 ttgcaaaagc tgataaaacc tacagcaaaa attatgatga aattctgata ctcttaaaaa
    18541 caggtcttcg tatttcagag tttggtggtt tgacacttcc agatttagat tttgagaatc
    18601 gtcttgtcaa tatagaccat cagctattga gagatactga aattgggtac tacattgaaa
    18661 caccaaagac caaaagtggc gaacgtcaag ttcctatggt tgaagaagcc tatcaagcat
    18721 ttaagcgagt gttagcgaat cgaaagaatg ataagcgtgt tgagattgat ggatatagtg
    18781 atttcctctt tcttaataga aagaactatc caaaagtggc aagtgattac aacggcatga
    18841 tgaaaggtct tgttaagaaa tacaataagt ataacgagga taaattgcca cacatcactc
    18901 cacatagttt gcgacataca ttctgtacca actatgcaaa tgcaggaatg aatccaaagg
    18961 cattacagta cattatggga catgctaata tagccatgac gctgaactat tacgcacatg
    19021 caacattcga ttctgcaatg gcagaaatga aacgcttgaa taaagagaag caacaggagc
    19081 gtcttgttgc ttagtagtac aaatgaattt actacttatt taccacttct gacagctaag
    19141 acatgaggaa atatgcaaag aaacgtgaag tatcttccta cagtaaaaat actcgaaagc
    19201 acatagaata aggctttacg agcatttaag aaaatataaa aagataatta gaaatttata
    19261 ctttgtttgt ctaaaaaagc agcaaactat aaactagtag gttccacacc aaatgtagac
    19321 ccatacttcc ccataagtcc gatttatagc gcaccatccc taaaaagatc ccaagtgaaa
    19381 catataaaca ccaagctaga atggttcctg gatgatgtac taaggcaaat aaaacacttg
    19441 tcaaagcaac tcgaatatc
//
LOCUS       DAVHIT010000210         9517 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 935-3147
            SAMN08166725-rid19298383.guided.079, whole genome shotgun sequence.
ACCESSION   DAVHIT010000210 DAVHIT010000000
VERSION     DAVHIT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166725
            Sequence Read Archive: SRR6382475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869600.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869600.1
            Genome Coverage       :: 247x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:50:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 71 of 132
            Pseudo Genes (incomplete)         :: 53 of 132
            Pseudo Genes (internal stop)      :: 42 of 132
            Pseudo Genes (multiple problems)  :: 30 of 132
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166725-rid19298383.guided.079"
                     /serotype="19 F"
                     /isolate="935-3147"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2016-03-24"
                     /collected_by="Pediatric Research and Clinical Center for
                     Infectious Diseases"
                     /note="genotype: ST 1834"
     gene            437..598
                     /locus_tag="VVN16_002127"
     CDS             437..598
                     /locus_tag="VVN16_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573414.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            690..2369
                     /locus_tag="VVN16_002128"
                     /pseudo
     CDS             690..2369
                     /locus_tag="VVN16_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2637..3203
                     /locus_tag="VVN16_002129"
     CDS             2637..3203
                     /locus_tag="VVN16_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573415.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3525..3713
                     /locus_tag="VVN16_002130"
     CDS             3525..3713
                     /locus_tag="VVN16_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU5573416.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3775..4533
                     /locus_tag="VVN16_002131"
     CDS             3775..4533
                     /locus_tag="VVN16_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5573417.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     ISEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKSYFEKNEIFKIQQDVIF"
     gene            4591..5445
                     /locus_tag="VVN16_002132"
     CDS             4591..5445
                     /locus_tag="VVN16_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5573418.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMCISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            5557..6441
                     /locus_tag="VVN16_002133"
     CDS             5557..6441
                     /locus_tag="VVN16_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU5573419.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            6604..8343
                     /locus_tag="VVN16_002134"
                     /pseudo
     CDS             6604..8343
                     /locus_tag="VVN16_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            8618..8920
                     /locus_tag="VVN16_002135"
     CDS             8618..8920
                     /locus_tag="VVN16_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5573420.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            8976..9404
                     /locus_tag="VVN16_002136"
     CDS             8976..9404
                     /locus_tag="VVN16_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5573421.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
BASE COUNT         3353 a         1321 c         2011 g         2832 t
ORIGIN      
        1 actgttaagt ataagagggt aatttataga attttgttac atcaagataa atattttctg
       61 atggtcaaat tttaaaaaga ttaataactc tcgataaatt ttgaacctta acaattgaat
      121 agaccaagac aaggcgttaa ttatttttgg agcgtaataa cctatccgtc aagatctttc
      181 ggctgaataa ttcggcaaaa aaagttctgc taagcaaaaa gaaagtgagc gataagtaag
      241 agtttgaata tagggaggga taccctatcc gctatgaaga aaatgaggat aatgatactt
      301 ctaaggttga agccggctca tccggaatag aggcggaggt gagattccta tgttgctaat
      361 ccaaagcact tgtcaatgtc aaaaaactta attaaacaaa taattattag atataaaact
      421 ttagaggagg tatatattgg acaatgactg gaatggattg gctgatttaa tagcaaatct
      481 gattgcaaaa tatgctggtg ctttagattt agataatcta cccgatccaa cgccagcaaa
      541 aaatcaagag atgaaaaata gttttgacat ggcaaaaaca caaattgaga cggactaata
      601 aaagtgttat aatatacttg aaatgatagt ccaaacttaa attaaaaaaa ttatatatga
      661 tatagaaaaa ttatattgag gttaatacta tgtcaaaaga aaaaataaaa gtatacctct
      721 atacacgagt atctacgtca atacagatag aggggtattc gttagaggca caaaaatcac
      781 gaatgaaagc ttttgctatt tacaacgatt atgaaattgt tggagagtat gaagatgccg
      841 ggaagtctgg caagtctatt gaaggtagaa tacagttcaa tcgaatgatg gaagatataa
      901 aatctggaaa agatggagta tcttttgttc ttgtgtttaa gttatcaaga tttgcaagaa
      961 atgccgctga tgttctttct acacttcaga taatgcagga ttatggagtt aatcttatct
     1021 gcgttgaaga tggcattgat tcatctaaag atgctgggaa actaatgatt tctgttttat
     1081 aagctgtggc tgaaatagaa agagaaaaca tccgtattca aacaatggaa ggtcgcattc
     1141 aaaaagcaag ggaaggaaaa tggaatggtg gctttgctcc gtatggatat aaacttgaag
     1201 acggcaagct gtttataaat gaggaagagg cagttgcaat aagaacgatt ttcgaccagt
     1261 atgtaaatac tacgatagga gctaatggga tatctaaata cttagagaat catggaatta
     1321 gaaaaatccc aagacagaat ggtaagaatc ctttgtttga tgcaggtctt ataagaaaga
     1381 tattaaagaa tcctgtatat aatgggaaaa tagcatttgg aagaagaact ttagaaaaag
     1441 ttcatggtac aagaaatgaa tataagcagg ttgaacaaga tgaatatcta atatctgaag
     1501 ggatacatga agctatagtt tccgatgaag tttggcaagc tgctcaggtt aagctaaaat
     1561 ctcaagcaaa gaaatatgag catgtgaata aaggaaaaga tacacgcaca cacttgcttt
     1621 ctggaattgt aaaatgcccg atatgtggag tgggaatgtt tgggaacaag tgtatcaaga
     1681 aaaagaaaga tggcacaaag tataaagatt tttattacta tggttgtaaa cataggcaga
     1741 tgataagagg tcataagtgt acattcagta agcaaattag agaagaattg ttagatgatg
     1801 ctgttgcaga ggtgattgtc aagatagtaa gtaatccgaa atttgcttct atgatgcaag
     1861 aaaaaatcaa catgaaggtg gatacctctg aaatagaaaa agaaatagat aattaccaaa
     1921 aggaattgag aaagagtcat tctacaaagt ttaagctaat tgaggaaata gataatttag
     1981 atgttgaaga taagcattat aagagaagga aacaggattt agatgataga ctatatcgta
     2041 tgtatgacaa aatagatgaa ttagaatcat cactaattga tgcgaaagca aagaaacaga
     2101 ctattgaagc tgaaaagctt acaggagata acatatataa ggttctgatc tattttgata
     2161 agctctataa agtcatgaat gatgtagagc gtaggcagtt aatttcagct ttgatttctg
     2221 aaattcaagt ttatgaagaa aaacaatcta acggacaatg gctaaaatcc attactttta
     2281 agctaccaat tatagaagaa aatctcaaca taggtttgga caatgatgaa caagttgaat
     2341 gtgtaagttt attagagaaa cgtagttaat cctcaaaagg ttccccgaac cttttgagtc
     2401 ccactgacgc atcacgctga ggcggtatca ctgcttgtac gagttgaagc atcagcaaag
     2461 taaaagtaga tgttcagcca atgcgacgag gtgagaccga aggtcgataa ggtgtgttct
     2521 gggtatagga ctcgtaaagt aagtaggaaa tatgatgaga tgatacagag agcctgaatt
     2581 ggtcaggtga cgtgcttatt tacattagcc tgataatgaa aatggagaga ttatcgatgg
     2641 ttatattgga attgtaccaa aatgattatt ctaaagattt agtagcattt gattccatag
     2701 aagatgggaa agcctttgta gctcaaatcc cgggatacac tctggaaaca gaagatggtt
     2761 ttgaagtgga gtattttaat cctaaaaaca taccggatta tatggaaatt atttttaacg
     2821 gaaatattgt tcctttatct agatttatgt tcgatcctgg ggaaaatgta aacattattt
     2881 ggaaagagat ttcaaattta tccctcaaaa atgatagagt gatcgaagga tattcaaaaa
     2941 ttgatgcata tgtcgttaac aaccatgaag ttaaagccta tgtcgaaaca agagaaacga
     3001 actatcgtaa agcaaaagac ttcttagaaa gccgcggata tgaaatcgac agaagctttt
     3061 tcggaagtga agacggtgaa gcgattcttt acagaaaaaa agggatcgaa gtttggcatt
     3121 tcttgtgtca cttagaccca atgtttgtag agattgaaga tgtagaagga tatgttaaag
     3181 aagaagttgg agagattcaa tagtcaacca aagttccttg tgagtatata cctttgctag
     3241 taaaagagtc aaaacgttat aacgaagggg gggaagaaaa cccttgagtg taagtctttt
     3301 tcttgtttag agggaaagta ttagagtagc cgctcataat ttatactaga tttgataact
     3361 gatttgagac agattttatt tctataaata ttgtgaaaat tttttaatac caaagattta
     3421 aagttactgt gttaaaaatc ctctccactt ttaaactaat gtattataaa agccagttga
     3481 cacaagtgtt acacctgtga tagaattaac ttgaggtgat gaaaatggca accgataaaa
     3541 atagaataat gattagcttg gatgataaaa atctagagaa acttgaaaat ttagtagaag
     3601 atgctagaga tagaagggga atgcgactaa caaagtccca ggttattgaa ttactcctaa
     3661 ataccgttga ttatttcgat gatattatgg gagctattta ctcaaaaaaa taagagcaaa
     3721 attgctatta ttttttacaa attggtgtaa cactagtgat ataggagtga tgaaatggtt
     3781 gtttatatta gacaaagtaa attaccaagt gaggtttcaa ttaataaata caatgctcaa
     3841 gtaggtgcct acttacaagg agaagaagtt attttatatc aatctttttc tgaaataaaa
     3901 gagttaataa gtgaagatat agttgtagat tatataatgg agactagagc attactaaaa
     3961 atgatgggct taaacgttcc ggtttatgac tatcctattg agcttaaaga gttttatggt
     4021 cgaaagattt atgctggtat tttaggagag attgtgaata tacctgataa ttggggaaaa
     4081 tttattaagc ctaaagctgg ttcaaaagtc tttactggaa gagttgttaa tggaacccat
     4141 gatttaatag gtattggtct acctttcgac tatcctatat ggattagtga ggttgtagaa
     4201 ttcatagctg aatggcgttg ttttgtgtta gatggtcgcg tattagatgt tcgaccctat
     4261 acaggtgatt atcatgcaca atttgatgca agtgtaattg atgaagcgat atcatgttgg
     4321 aaagatgcgc caatagctta tggactagat atcggtgtta ctcgcgatgg cagaacactt
     4381 gttgttgaag taaatgatgg ttatgcattg ggaaattatg gcctatctcc tttaaaatca
     4441 atcaattttc atagagctag gtggaaagaa atggtaaaat cctattttga aaaaaatgaa
     4501 atttttaaaa tccaacagga tgttattttc taaaataaat atggtagata accaatataa
     4561 aaagcatcaa aatgaacaag gaggaatctg atgcttggag caattattgg agatattgtc
     4621 ggttctgttt acgaatggaa caatatcaaa acaaaggact ttcctttatt tcggaaggac
     4681 tgctttttta cagatgacac ggttatgacc tgtgctgttg cagaagcaat tatgaatggt
     4741 ggacaaaaag acgatttcat tgacgcgatg aagaaatatg gcagaatgta tccgaatgct
     4801 gattacggtg ctcggtttaa tgcatggcta aacagcgata accgtgagcc ttataatagc
     4861 tttgggaatg gatcagctat gtgtatttct ccatgtgctt ggatcatgga ctgtggtttt
     4921 tatgcgaaaa ctggtatgtg gccatcatct agaggactta cgagtctttc tgcagaggta
     4981 actcataatc atccagaagg tgtcaaaggc gctatggcta cagctgatgc tatctttcta
     5041 tgtcgttttt actttggtgg ttattgtaga gagtacgagc aatcaattaa cgacaatcct
     5101 acagagtgta aaagacgaat taaggattat atagaaaaag aatacgatta caatctatct
     5161 caaactttag atgaaatccg ccctaactat cgttttaacg aaacatgtca ggaaactgtc
     5221 cctcaagcca ttatcgcctt tcttgaaagt agagacttcg aagatgcgat aagaaatgcc
     5281 atctcacttg gtggcgacag tgatactctc gcagcaatca cttgtaacat agcagaggca
     5341 gcttacggta ttcctgattg gatcaaggat aaggcctatt cttacttgga tgaaccctta
     5401 aaggatgtag ttaggcgatg ggaaaataga ataaaagcgt attaatctcc agtacgtcag
     5461 atttctcttt tctgtatttt cattgtataa taggattaca tttacttgca tacgacgaaa
     5521 tgtaaacatt tctgtttaaa attaaggata aaatcaatga aaaaagcaat ggtaattatc
     5581 aaccctactt ctggtggcga gaaggctttg gattacaaag aaaagctgga gaataaagca
     5641 aaagaatact ttgaatatgt tgaaaccaaa attaccgaaa aagcgctgga tgcaacacat
     5701 tttgctgaag aagcttctcg tgagcagtat gatgcagtgg ttgtgtttgg tggagatgga
     5761 actgtcaatg aagtcatttc aggtattgat gagagagact acattcctaa gttagggatt
     5821 atcccaggcg gtacgggtaa cctcattaca aaacttttgg aaatcaatca agacatcgat
     5881 ggcgcaattg acgaactgga ttttgattta accaataaga ttgatatcgg taaagcaaat
     5941 gacaactatt ttggttatat ctttagtatc ggttctctgc ctgaggcgat tcacaatgtt
     6001 gaaatagagg acaaaacaaa attcggtatt ttaacctatg ctgtaaatac catgaagtct
     6061 gtcatgacag atcaggtctt taacattaag gttgagacag aaaatggaaa ttatgttggt
     6121 gaagctagcc atgttttggt ccttttgaca aattacttcg ctgataagaa aatctttgaa
     6181 gaaaacaagg acggctatgc caacattttg attctgaaag atgcctctat attctccaaa
     6241 ttatccgtca ttcctgattt attaaaaggg gatgttatcg caaatgataa tatcgagtat
     6301 atcaaagcgc gtaatattaa aatctcttca gatagtgaat tggagtcaga tgttgacgga
     6361 gataaatcag ataacctacc tgtagaaatc aaagtcctag ctcagcgagt agaagtattt
     6421 tcaaaaccga aagaggatta gtatatagag aaagcctttt ttaaggcttt ttgtatactt
     6481 taaaagatag ttcctttaac aacggacatt ccttgcaaat agtttacaaa aatagtatac
     6541 tggattcatt gagtttgaaa acgtttgcgt aaaatttgaa tgaatacttt aggagacaaa
     6601 ttgatggaat tgagtgctat ttaccatagg cctgagtcgg agtatgccta tctttataag
     6661 gataagaaac tccatattcg aattcgaact aagaaagggg acattgaaag catcaacttg
     6721 cactatgggg acccttttat ctttatggag gagttttatc aggatacaaa agaaatggtc
     6781 aagataactt ctggtacctt atttgaccat tggcaggttg aagtgtcagt tgactttgca
     6841 cgtatccagt atctctttga gctcagagat acagaaggtc aaaatatttt gtatggcgat
     6901 aaagggtgtg tggaaaattc tctagaaaat cttcatgcaa ttgggaatgg atttaagttg
     6961 ccttatcttc atgagattga tgcctgcaag gttcctgact gggtttcaaa tacggtatgg
     7021 tatcagatat ttcctgaaag atttgccaat ggcaatgctc tattaaaccc agaagggact
     7081 ttagactggg attcatctgt cacacctaag agcgatgatt tctttggtgg tgatttacag
     7141 gggattattg atcatatgga ttacttgcaa gacttgggta ttactggact atatctttgt
     7201 cccatctttg aatctacaag caatcacaag tacaatacga cagattactt tgaaattgac
     7261 cgtcattttg gagacaagga gacctttcgg gaactggtgg atcaagcgca tcatcgtggc
     7321 atgaaagtca tgctggatgc ggtatttaat catattggtt cgcaatctct tcaatggaaa
     7381 aatgtcgtca aaaatggtga acagtctgct tataaggatt ggttccatat tcaacaattc
     7441 ccagtgacaa ctgaaaagct agttaataag agagacttac cctatcatgt ttttggtttc
     7501 gaggactata tgcctaagct aaatacagcc aatccagagg tcaagaatta tcttttaaag
     7561 gttgcgactt attggattga agagtttaat atcgatgctt ggcgtttgga tgtggctagt
     7621 gagattgacc atcagttctg gaaggatttt cgtaaggcag ttttagctaa aaatcctgat
     7681 ctttatatcc taggagaagt ctggcataca tctcagcctt ggctaaatgg agatgagttc
     7741 catgccgtca tgaattatcc tttatctgat agtatcaagg actatttctt acgaggaatt
     7801 aagaagacag accagttcat cgatgaaatc aattgagagt ctatgtatta caagcagcag
     7861 atttcagagg tcatgtttaa tctcttggat tcacatgata cagagcgaat cctgtggacg
     7921 gccaatgaag atgttcaact ggttaaatca gccttagcct ttctctttta acaaaaagga
     7981 acaccgtgca tttattacgg aaccgagcta gccttgactg gaggaccaga tccagattgt
     8041 cgtcgttgta tgccttggga acgtgtatca agtgacaatg atatgctgaa ctttatgaag
     8101 aggctgatta agattcggaa atacgcgtca gtaatcattt cgcatggcaa gtatagcctt
     8161 caagaaatca aatctgatct agtagctctg gaatggaaat acgaaggatg gatcctcaaa
     8221 gcaatattca accaatcaac agaagattat cttttagaga aagaagcagt agcactagca
     8281 agcaattgcc aagaattgga gaatcagctt gtcatctctc cagatggatt tgtgattttc
     8341 taaaaactag ttgatgaaga ttatggtaca tttcatatct tatatagtat aataaggcta
     8401 gttactaaac ttgtaaagga gaacttaaat gaattgtaga ggacatgaaa caagacaaag
     8461 aattgttaga gattttgaag tttagcctaa agcacatatt aagctgttag caaatcaaca
     8521 aaaacatagt gatgcaggag caactattga agatgaatat tatgtattta tcgctgagag
     8581 taaaattgat ggcaagaagg aagttattca gtgttgcatg ggtgcggcaa gggatttttt
     8641 agaactaatt aatcacaaag ggctacctct ttttaatccg cttgtaggtg attctcatgt
     8701 aaataataga caagaatatg acaatacagg gagtggaaat ttacaacctg aaaagtggaa
     8761 tgaaactgca aagcagcttt ataatgttat aatgtggttg attattttat ggaatgctaa
     8821 gccggataca cctttattta attttaaaga cgaagtaatt aagtataaaa catatgagcc
     8881 ttttgaaagc agtataaaat tggtatcaat caatgagtaa caaagtatca gcatgattcg
     8941 aaacatttac ttgtaatttg cggaggaaca tcaatatgga tggaataaca aaagatttta
     9001 taaaaacggc taaactaatg aaacaattat ggccccaatt gaccgataaa gaagctattg
     9061 atgaagtaaa aaaatatacg aatggcaaaa atactgcaat ctttactgaa gttgaaggtg
     9121 acacaattgt aggtctagca ctatgttcac tcagatttga ttatgttgaa ggttgtaaat
     9181 atagtcctgt tggattctta gaagggatta ttgttgacga ggaatatcgt ttaaaggata
     9241 ttgctaaaaa tctctgtaca aaatgtgagg aatgggcgaa aaataaagga tgtaaggaat
     9301 ttgcaagtga ctgtacttta acgaatacgg attctataag atttcatctc aatattggat
     9361 ttcaggaggc aaatagaatt attcatttta agaaaaaatt ataatttaga aacgtgaata
     9421 aacttgttta aactttatgt atctttagaa agtgtgttaa cttgaaagaa caaatttttt
     9481 tgatgggtgg gaatccccca atgaagaaat atagtat
//
